LOCUS       DAWKST010000001        39784 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.001, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000001 DAWKST010000000
VERSION     DAWKST010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 39784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39784
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.001"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..636
                     /locus_tag="WJL87_000001"
     CDS             <1..636
                     /locus_tag="WJL87_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ7798502.1"
                     /translation="NRQLTEKNLKIEKASAAVIDATIIQTAGSKQRQAIEVDEEGKVS
                     GQTTPSKDKDARRTKKNGLYRLSYKQHTRTDEEGYIEKLHITPANTHECNHLSPLLEG
                     IAEDTTVYADKGYDSKENRQHLKEHRLLDGIMRKAQRNRPLTEAQTKRNRYLSKTRYV
                     VEQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMCLNLLKAANRLSVPVAA"
     gene            1180..1548
                     /locus_tag="WJL87_000002"
     CDS             1180..1548
                     /locus_tag="WJL87_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEZ7798503.1"
                     /translation="MIDGELVALIHQTVLADEAGLKGRADMACLDGALSRIANWRQYE
                     NLEDIYEIAALYAQAIAKAHAFPDGNKRTALLTMLTYLDLQGISIAADQGLDDLIVSL
                     AAGETDFKQLAETLRRLDKE"
     gene            1986..2477
                     /locus_tag="WJL87_000003"
     CDS             1986..2477
                     /locus_tag="WJL87_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HEZ7798504.1"
                     /translation="MFWQLTVVSVTAVIALGTIFINKKTSKQKATLDVILNDYQDAQF
                     VEADNHISPYIRGTAVDDNNARIDLYEIYQNKGGQWEKERGHLLTVINRHEFYACAIN
                     SGVLDEDLFKRLHCTNFIKLWNAVSPLVMKIREEERKDTIFRELEILVALWKANPLKA
                     SDL"
     gene            2836..3072
                     /locus_tag="WJL87_000004"
     CDS             2836..3072
                     /locus_tag="WJL87_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEZ7798505.1"
                     /translation="MLRVQKWGNSAAVRLPADMLKQLDFKIGDALVAEVHNGELRVRA
                     ARRFRLADLLAEMEETPPRVEGWEILDDVGNEVV"
     gene            3074..3421
                     /locus_tag="WJL87_000005"
     CDS             3074..3421
                     /locus_tag="WJL87_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ7798506.1"
                     /translation="MYIPDKGDIFHLNFDPSSGKEIKGGRFALALSPKAFNRATGLVF
                     ACPISQGNAAAARSSGMISTLLGAGTETQGNVHCHQLKSLDWQIRKASFKETVPDYVL
                     DDVLARIGAVLFD"
     gene            complement(3474..3983)
                     /locus_tag="WJL87_000006"
     CDS             complement(3474..3983)
                     /locus_tag="WJL87_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798507.1"
                     /translation="MDILLKYWKPVGVLLLIVLIFTAWHFDRAEKYRMGREAAAAEIS
                     NRLKDGYIEQAKQARSAEQKAAAAFAERQTKLEEEKQNAEKTVAAMRLELNRLRHYAA
                     AQNRNRNLPATTTAATASDGASDSQGWLLFGQCAEKYAGLAETADGHAADLREWQAYG
                     HAVSSQRAE"
     gene            complement(3995..4468)
                     /locus_tag="WJL87_000007"
     CDS             complement(3995..4468)
                     /locus_tag="WJL87_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ7798508.1"
                     /translation="MQVTKNFSLRELTRSETARRAGVPNEPSPADMDNIYYTAQQLEK
                     IREYVGRPIIVTSCFRSERVNKLVGGSPTSAHRHGLAADCDASGMTSPAFAKLLIKMR
                     DEGALKFDQLILEFPERGDGAWVHIGFRRNSPQRNQILTATKKNGKTVYLPGLHP"
     gene            complement(4607..4882)
                     /locus_tag="WJL87_000008"
     CDS             complement(4607..4882)
                     /locus_tag="WJL87_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798509.1"
                     /translation="MKLLLILSAAPSLISAYLMICRLNGRKWSALSPEAWAYLAFLGG
                     AIYTFYIVFAYGLTPSLGKFMMDIAACVYFGSRSVRVVKWRHRHGLK"
     gene            complement(4879..5304)
                     /locus_tag="WJL87_000009"
     CDS             complement(4879..5304)
                     /locus_tag="WJL87_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798510.1"
                     /translation="MSEPIITPAAVYIFGGVVGAGYLLGMPVDAVVLGAVASAAVTMI
                     SEPKSRWMVVAYIVIGGLLGGAFAPPLTHFLIGEVVGSHPSLGEQRIQFAHAFAPVVV
                     GLLWQVLLRFARAVWPSFEKHADDIVEWLLNLLPWRPKK"
     gene            complement(5368..5778)
                     /locus_tag="WJL87_000010"
     CDS             complement(5368..5778)
                     /locus_tag="WJL87_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798511.1"
                     /translation="MVQTVFFYPKETIMSKDKNQITVGINREIIDTETDSSAEFHVIR
                     HVSLDYATGFNVVLLDSYVRKSTFERRGRSVGSMQLNIPGVPPRGTDVLDWAYQAVVA
                     AVEGGAVDAYGQPISANSLTGAELVYADLPEAAI"
     gene            complement(6030..6476)
                     /locus_tag="WJL87_000011"
     CDS             complement(6030..6476)
                     /locus_tag="WJL87_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798512.1"
                     /translation="MDQAKRTAYVKIINALFDGAVKVQPSQITETVVDMVDKMFDEIA
                     TCHERIRPMAVLADGIAGSIVKLAPNEIPLIFGNSSFGSYLKSKTRDEVFKVLKQSKV
                     NNAGKVLEAFYESWLKYTAKDRRLKVCIVTAQSRWRSRFEMELLGI"
     gene            complement(6547..10812)
                     /locus_tag="WJL87_000012"
     CDS             complement(6547..10812)
                     /locus_tag="WJL87_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ7798513.1"
                     /translation="MGGKSGGGQRTPYEAPNSLSSAQSLRIIDAVSEGVVSGFANGDD
                     APFKSVYFDDTPVQNSDGSYNFNGVTGYFQRGEQDQSYVPGFDASERTVAVSAAVKQN
                     QPMVRAVTDELVSRLRITVAVERNAQVEDNGDTVPADTLMRVELINSNGVQASNQVRF
                     NEKSSGTYYQDVEFDVVPQVPFNIRVFRISPDSKTDKVSNNTYFSSYVEIVDAKLSYP
                     HTAFAALSIDSAQFGNQIPRRNYLMKGRLVKVPSNYNPETRQYTGGTWDGSFKTAWTN
                     NPAWVFYDVLTQPRFSTLARRLNVADIDKWSLYQIGKYCDEPVDDGFGGKEPRFVCNA
                     YITDLMQAGEFLNNLASVFTGLPVWNGQQVSVVMDADADPVAQYTNANVKDGLFNYAG
                     AALKSIHTAVHVQYIDKYDGYRAKTEYVADNEAIARYGLNIKQITAFGCDSRGQAARF
                     GAWTLQTELRQQNAVTFEIGREGLKHLPYDIVQIMDNQYAGAELSGRVAAISGNVLTL
                     DRPVSDAVGALVFYSEGGSLKSAKITAAAGNKVTLEREVPLQAGDTWVLSGKVKPRLY
                     RAIGIKENTDAGTYTVTALLHDPKKYAAVDSFAHFDHEITTLHNTAPVLTDAAVNTDG
                     GTVVVTWDNLAANGQVLTYDIKIYRNNSLYRHIPDAQTAEIRLENLPNGNYRAEIRGR
                     NARGVLSAPLVKAWSIDYTVTGLRTTPKTLSVTVDWVLPQTVVSDLVFELWYSKTNNL
                     QTATKLASLAYPQNSYTLTGVGVADVYYFWVRIRDIAGNTGEFTAAVQGRADNNPAPI
                     VQQIQGAIGKSALSRELIDTLNRDMAAAAGLKVSAEAAERAKALQEEARARTAAIQAE
                     SSARTAELARKSSEIGTRIAATEAVNEQQAQQIQTVTSAQGNTAAGLEAEKKARTDGD
                     RAEAQARETLAVRLGNAESGISALRETVTRQDAARSSEINTLTAKLENVEVGGRNYAL
                     STGTAGKVLTASENNQTKTVTIDVSSALDLKQGDSLIISCDIELVNATSPHGKPYPRI
                     GAEFSVIYADNSVGYFAAWYGEAVSGTTKTLKQRIVAKHTVAKEVKALRSFIVQARYQ
                     TSESIKVSNVKLERGTVATDWSPAPEDSNADTSALTTLSAKVQQASSAVAEIGGKIQS
                     LYTLKTEAIAGGRRAVAGVAVGADGKTGSGEILLMADKVAYVDPKDKTVTPAFVTVIE
                     NGKAKQALNGDLFATGSILAKHIAANQTIEAPTLNGATVNAGLIKGGTIRGARIEAVD
                     LEAANIIGDVVAVRTFELKQSPLGYQELICQIPDSRKTRRTVIVPPVFAVAENGAVVS
                     LQLHMDGVIQREVTIQSPEKRIARNEKFSVWVPGPPPLYIRTPQEVPLSFSVPFHLPK
                     TGSISFQKGIDGAAHSLHILCRISGNFDYSPLGDLSGIVCLIV"
     gene            complement(10948..11670)
                     /locus_tag="WJL87_000013"
     CDS             complement(10948..11670)
                     /locus_tag="WJL87_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HEZ7798514.1"
                     /translation="MITVCLYGGLREYGRRFVLHVETPAEALHALFTQIKGLRQRIRD
                     GVYQVRFDGKDQSEETIGSVFRRPADGVLHIVPRVQGAGKNGGLIQTIVGVVLIVVGA
                     YTSWASGAQLIAVGIGMVAGGVAQMLTKQPKLDADGKGVEESKNSAFSNLANTTAQGR
                     PVPLAYGLCYCGSRVVSQGVQSRRIEPSSTAASSGAVLNLGLSKTFVQGVAATAPNGQ
                     KYRTDFSDDSVRARNYKAAFSG"
     gene            complement(11667..12422)
                     /locus_tag="WJL87_000014"
     CDS             complement(11667..12422)
                     /locus_tag="WJL87_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ7798515.1"
                     /translation="MMDLDLLSEEARREMLACAEEAVPSEMCGVLVFSYEGYEFLPLS
                     NCAENPHETFEISADDWMAAERVGEIVAVVHSHPRGEPFLSGADRWMQVETGLPWILV
                     TQGRLKLFRPVPHLRGRVFEYGKTDCGALVRDAFMLMGLVFPDHPRGDMDEDAAAGFW
                     EKHLENCGFSRVSDGLCAGDVVLTATGGHASHAVLYLGNDWMLHHAYNQLSCRVPYTR
                     YWADVTHSVWRHPDFEPAMMQALENDFTHMEQA"
     gene            complement(12419..13120)
                     /locus_tag="WJL87_000015"
     CDS             complement(12419..13120)
                     /locus_tag="WJL87_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015971142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HEZ7798516.1"
                     /translation="MSGVMLQALSAAQQDVLVDLWQVDLRPLGGRVFYFCNQVNERGT
                     AVVWQGQAYEPYPIKADGFESTSQGAGNRPTLTVSNLLGLITGAADQFGQLVGVLVVR
                     RQTYAKFLDAVNFQSGRNPTADPMQEVVGKYLVERMTALNAETATFELAAPSESDGSV
                     IPARIMLANTCCWQYRGEGCGYTGRAVADRFDMPTDDASKDVCSKTLTGCRARFGATA
                     VLPFGGFPSSDKVTT"
     gene            complement(13201..13470)
                     /locus_tag="WJL87_000016"
     CDS             complement(13201..13470)
                     /locus_tag="WJL87_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ7798517.1"
                     /translation="MTETFRWRVASDNKAVHKFDVRSVRFGDGYEQRQPKSLKPKLRS
                     WEIKIVGQKALMGEIKAFFDARRGVEPFNWRPPDGVPVLVKVSEY"
     gene            complement(13492..16710)
                     /locus_tag="WJL87_000017"
     CDS             complement(13492..16710)
                     /locus_tag="WJL87_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HEZ7798518.1"
                     /translation="MNNETKIYITAETGGVVSGVEKAKQSIKSLGDVASSQGRRISDG
                     LVHSGDGAEKSSTVVSAASKRTERSLASLENAIRRDIAVKIAGGKANREYYEELARQR
                     GIDIARLNPLLSQLDRHNTQTNRATQSVKQFNNALRQTPAQITDIVTQLAGGQSPFLI
                     MIQQGGQLRDMYGGFGGMLKGLATVISPMRLAVAGLGGGIAALGYAMYQGAEESCEYR
                     KALILAGDAAGITADRMQEIAVSVGAATGGYADARAAITALVSSGKVATDNYEQFARS
                     ITLQSQATGQSIDDLVEKYTEIAKDPLKAVVSLSATYRTMTADVYQQVKALQAQGREQ
                     DAVALVQRKFSEESEDMAKRVLGNLGLIERAWKDIKESASEAWDAVKSIGRDKTKLDE
                     IAALDSFISQIETSKKHPVTQLFWGEEGERKLQEAYARRARLRAEIDKEAAAAETLRQ
                     KQKQNQNRTEGIAELEAVSERYATREQQRQRELLKNDKARKKALDGITDAKKRQEIIN
                     KHAANAKLINESFAEKKHERQKEKTDKTSYPTVSNGLRIKPGAEAGGKALGGTYAFAH
                     AMQQMLGDRLFRFGAFRDRYHIGKNSLHNSGLAFDATPSPGMGMKQKQEVVAQMTRYL
                     ESLGFKNGQDFNLKLELAGQRNKNGTVATGDHWHFNWRSREAAARFAGGVEGQVKNMA
                     HLGLFKGVEKEPLSGYEKWHEDFNRRAAEAGAKRVLEILNGNRAIGEQLKLLSDPTFG
                     DWTLKQQADARELAEKADAQDSLTAASKKYSDIVKQMTDDSKEKLDDRLFEISLIGKT
                     REEIEKLTLARLWDRQIAKAREEGAPLESIDLLERGKAEGMSNLSQMQKARADSDNDW
                     RGGIESGLKSYIDSFGTMRQAMENATVQTFDKMGDALADFVATGKLDFRSLTVSILQD
                     LSKMLIKMAIVNAMKSALGYADGGIVGGGSTQFDALFSGGGYTGYGGKYEPAGIVHKG
                     EVVFSQRDVRNHGGVAAVERLRLNGYAGGGAVGLPSVLTGVRSAGAGSMQVNITINRD
                     GSTESDSSADTEMAKHLAAAIPGMVEQWYVKNVYRENGTYHK"
     gene            complement(16703..16975)
                     /locus_tag="WJL87_000018"
     CDS             complement(16703..16975)
                     /locus_tag="WJL87_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1799 domain-containing protein"
                     /protein_id="HEZ7798519.1"
                     /translation="MNVFGFSADDFSEKETTFGVWPCNWQAVQLFIGVSTQWRIGMSG
                     ATGLDYSAVAAVMECGNIKSKKRKALLEKVRLMELEVLSMWAGEHE"
     gene            complement(17023..17334)
                     /locus_tag="WJL87_000019"
     CDS             complement(17023..17334)
                     /locus_tag="WJL87_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HEZ7798520.1"
                     /translation="MAKLTLKPDATFRHTVKIPVPGAEPADVEFEFKARGRKAMKEFT
                     EKHKDGWTADTVLDCVQGWDLEEAFDRQNVEILLDSYPMAVFAIVNGYVEEVFNAREG
                     N"
     gene            complement(17398..18039)
                     /locus_tag="WJL87_000020"
     CDS             complement(17398..18039)
                     /locus_tag="WJL87_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ7798521.1"
                     /translation="MAVKLPNGATVHIATALAAEKKATVATNAAECVLTVAGHGFANG
                     DLVLFKSGWGKLNERVFQIGDVKADTFKLTGIDTSNADEFPAGSGIGAVQKITDWVQV
                     SQIVEFSTSGGEQQYVDFGFLEDDFDQQIPSTKSAMSMSIKIADDTSLSGYKAAAKCS
                     DKGGKWPLKVVLKGGGLICYNGYPSMNKTPELVRNQVMAVTLSYAISGEVNRY"
     gene            complement(18071..18490)
                     /locus_tag="WJL87_000021"
     CDS             complement(18071..18490)
                     /locus_tag="WJL87_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798522.1"
                     /translation="MTSRLTEIRNAATDVLKTKFQRVYSGRGFAPAQSQLPCVVVYVD
                     RRRTEQETFDFPPTYRHTVRLVTLVCVQAASGADELAEEMLSLIGQLFAEHSDLGVGD
                     LENLVPDLLNIDSDESGEAVTVYYQQGWQAIYFDTAV"
     gene            complement(18471..18773)
                     /locus_tag="WJL87_000022"
     CDS             complement(18471..18773)
                     /locus_tag="WJL87_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798523.1"
                     /translation="MFNEPLNVFTNPADFGETVMIGGKEVNAIFDREFITDSGFGVAV
                     ANADPQIIVTEDDLPEDVKSVVVTVRGKRYAVAETDFDGCGMVVVQLRAIHDKPTY"
     gene            complement(18766..18993)
                     /locus_tag="WJL87_000023"
     CDS             complement(18766..18993)
                     /locus_tag="WJL87_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798524.1"
                     /translation="MAKIKIIPTRSFFDGEQTFATGKKYSVDESAAAVYIREGWAAEA
                     PAKEAESPVADGGEAEDSGNASGEGGEDGSV"
     gene            complement(19051..20943)
                     /locus_tag="WJL87_000024"
                     /pseudo
     CDS             complement(19051..20943)
                     /locus_tag="WJL87_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245550.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(20924..22486)
                     /locus_tag="WJL87_000025"
     CDS             complement(20924..22486)
                     /locus_tag="WJL87_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEZ7798525.1"
                     /translation="MAKQSNAAPKKRGFFARLFGRREAVPKTARRNFAGARPVGSLAS
                     WQPQNWSADALARSDLDRLRTRARSLARDNDYMRKFLNMVESNVIGRDGFALQMRVLL
                     DNADKPDALANKAIEAAFSRWARRGVCDVTGQMSFTDLQRLLIRSVARDGEVLIRHIS
                     GFDNDYGYALQVLDIDRLDTGYNVPQQNGRNAVRMGVELNSYSRPVAYWLRTAHPGES
                     YGQTNTGNLRERVPAEQISHIFLHDRPEQRRGFPWVASAIIGLQNLSGYQEAAIIAAR
                     VGASKMGFFKQTEDADNFMPPIDGQEVDNGRGSIDLIDSVEPGTFHELPQGYDFTPFD
                     PDYPHANYDAFVKASLRGIASGLNVAYHSLANDLEGVNFSSIRSGTLEERDTWMTLQN
                     WFADAFLYDVFDRWIEAALLMGAIKMPSGKSLPAGKLDKFKACNWQGRRWSWVDPLKD
                     INAHKEAVALAVKSRRDICAEMGLDFEDVITQIEQENQMLAGKGIIADVKPAASAAEP
                     ESEDNPNEENEA"
     gene            complement(22495..22749)
                     /locus_tag="WJL87_000026"
     CDS             complement(22495..22749)
                     /locus_tag="WJL87_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798526.1"
                     /translation="MSGHDYAVELEEAREILNSLRKAYKEHAAGRGMTKRYKIKDREM
                     EFSSLADLLKQIRFWQQEIKRLEAAAGVSPRRSGRIITRF"
     gene            complement(22736..23122)
                     /locus_tag="WJL87_000027"
     CDS             complement(22736..23122)
                     /locus_tag="WJL87_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HEZ7798527.1"
                     /translation="MEKQDNITPPHYRSRAVECIEFTERLNFCMGNAFKYVWRHREKN
                     GAEDLKKARWYLQRQLDSCAVMHLLEPEEYAELMDGLEKCELDDLMQHVLEEILYHAF
                     FESEDSLLAAMCGVSELLKGYGDERT"
     gene            complement(23125..23334)
                     /locus_tag="WJL87_000028"
     CDS             complement(23125..23334)
                     /locus_tag="WJL87_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798528.1"
                     /translation="MYDVFKTRAAELSGAFCSLDFWLCHKFVPLCHALCHFLTRRDID
                     TLIEEGLVEERFEQGVSKFYAKEAV"
     gene            complement(23351..26197)
                     /locus_tag="WJL87_000029"
     CDS             complement(23351..26197)
                     /locus_tag="WJL87_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ7798529.1"
                     /translation="MNKKYATKAEINAAAHDRWREVLEACGIPSEILDKKHHPCPSCG
                     GSDRFRFTDGSGSSRGSGVWICNQCKPEGGSPFDLLMDVCGYSFNEARDKVASLLGLA
                     HGDLVDKAPKLLPPPAPVKEEERDLWKPIVPVPEYALNSMSFGNGFRKSDDLIFKSVF
                     RDSSSAVLGGVARFRKSDGGKIDLPYTFCEHKKTGEKMWRWRCWDNPRPLYGLDSLVA
                     DPSLPVLVVEGEKCKNAADAQGYNYAVLTWHGGCNNWDKSDWSAVVDRDVVLWPDCDS
                     LRQKLTKKDRDDGVDPESKPFLPRNEQGGMKAMLGIAEVLTKQNCRVWLVNIPAPGIW
                     PHGFDIADAIADGGRICDPSEVLSWMGAADWLVEYVPEAEMPSENSPAPIQDMEPESE
                     QLHESLEATGNQGGAGDVDDEYLAKLQKLKSEFGLVEGKDHGVNRKTGVTYTRKAMTV
                     HFGKDVVDAWYEWGRAPVLTMYEINRLKKDYALIQAVKDDDTQDMMERFIYLDGSASI
                     WDNKLWRVVPEKAARLSMTPDGFKIWVNSPSRIVKRFDRLVFEPGRVLSDDYINIFRG
                     MPIEPKFPIPKEDMPETNYELYRLFPEIKPILNLINHLCNGDYQQVEYILNWLAYPLQ
                     NVGAKMTTAVVMHGHIHGAGKSFFFEEIVKPMYGEYGATYGQEDLESNYTANRSGKMF
                     GIFEEVYTNQQKYNKTGSQKHMITGKTMRVERKFQDAYEEANHMNCVFLRNESHPFKI
                     EENDRRYFVVWPEKKLDKKLRDEVLDCIGNDGVRLFYSFLLCWNFYLTYGQQGAENEK
                     GYEVIKLEEPVRFDPNTPPPMTAAKQNVISYGRYAWQTFYSEWAGGGIKDLPFCCCIT
                     DDLWLVYKEWCRQNGERETGKTKFLQHIAEKMPRARRWWRTKNKDGDVSCQNWIFKPA
                     GWQAADGLPEMDALGNEVLRFRSAGWRYRGGEEV"
     gene            complement(26312..26659)
                     /locus_tag="WJL87_000030"
     CDS             complement(26312..26659)
                     /locus_tag="WJL87_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798530.1"
                     /translation="MNRTKTCKECGEIKPITAFPKNGFDRKTGLQKYAPRCRRCHNVY
                     QQDLLYKRREKEFEQKQAVMAALMDAEREKEEAAKARSIAQSLVLAKAACPILHIGLW
                     TEPLFIPQGMLQI"
     gene            27059..27775
                     /locus_tag="WJL87_000031"
     CDS             27059..27775
                     /locus_tag="WJL87_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7798531.1"
                     /translation="MTMHETTDRLFEIAKEQGVLKPADIAERLNISQQALKNWESRGI
                     AAKALPEVAKAFGVSETWLRTGEGSRTAPVLIDPDLPHEVKDIHRPMTWSSNDPLPDD
                     DYVFVPYLKESCFKGGVGTYEIPDYNGYRLPFGKSTLKRKGINPDNVFCCTLTGDSME
                     EKIAEDAAIAVDTGETAIRDGKIYAFAQDGMFRVKYLIRQPGNSVLIRSHNSGFYGDE
                     NAPLDSLTVIGRVFWWSVLD"
     gene            27878..28303
                     /locus_tag="WJL87_000032"
     CDS             27878..28303
                     /locus_tag="WJL87_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798532.1"
                     /translation="MNFLGVQGELKVHELHHRTATLCFEWLGEVSAPRRKENYKDLKP
                     NVLYDFDGSGKHFANPDARYLLPVGSSGLILKHIQIDDEDTLLRLWCARNIPMPHRLS
                     KIPMLRQYYLSKAWHEIYAINQHLRKTKLIVDVAYGPTD"
     gene            28534..28734
                     /locus_tag="WJL87_000033"
     CDS             28534..28734
                     /locus_tag="WJL87_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798533.1"
                     /translation="METITIPKENFDALLNLARTAVALNENFIPKINYGASTIPAAGF
                     AALNEFGIARSTVESLEILKIK"
     gene            28802..29161
                     /locus_tag="WJL87_000034"
     CDS             28802..29161
                     /locus_tag="WJL87_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798534.1"
                     /translation="METQEKKFVPASKQIPTVNSGWLDRELGEAMSEAVRACLAHGKQ
                     SEITVKLKIQPQNIQSGTVKISHDVATKLPKEKREGGIVFATPDGNIQADDPAQGKLK
                     LKQVPNTSSTLKMVRSN"
     gene            29186..30040
                     /locus_tag="WJL87_000035"
     CDS             29186..30040
                     /locus_tag="WJL87_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HEZ7798535.1"
                     /translation="METQENMIKTALQAAQKPFFEFAPNNTLLVFTPDQDGGWRYKSH
                     PELMQNPYRKCGKFLMHDTASLIKFVQKHKQDGTQIYIDADFKSGRIDVTAVINGHTQ
                     QAPGWLDFYAVYTPQHTTSASNWLNNNAHRMNQMEFSHFLTNNARNIVSKNPGNENSV
                     YPTAAEVLDFALNLEYTEKTTFKQGYREQDGRINFTFQSEDSGQTEKNLKMFERFGIE
                     FTPHQGGASYFVEALLKFRIDKNSGALVLWYELQQIDAVIEQAAKDISEAVQKAFPEI
                     DIYFGIIA"
     gene            30112..30327
                     /locus_tag="WJL87_000036"
     CDS             30112..30327
                     /locus_tag="WJL87_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798536.1"
                     /translation="MTITEEYSIDIRVTAEQGKNDYGYPTERYGCDIINTDGELLVGI
                     EPEYKTPFAAVRKALICLAKDNLKNAA"
     gene            30363..30623
                     /locus_tag="WJL87_000037"
     CDS             30363..30623
                     /locus_tag="WJL87_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798537.1"
                     /translation="MKYLIRTALLAVVAAGIYACQPQSEAAVQVKAENSLTAMRLAVA
                     DKQAEIDGLNAQIDAEIRQREAEELKDYRWIHGDAEVPELEK"
     gene            30620..30913
                     /locus_tag="WJL87_000038"
     CDS             30620..30913
                     /locus_tag="WJL87_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798538.1"
                     /translation="MNFQEENENIKNDIEINLLHSAEADSVEAMMDLAVYGLAAMVAK
                     HNKTRGEQLVLQAIAGHMRKLLAALPETESLVQTGKVYRRLEDLIFKTYLPEQ"
     gene            30916..31107
                     /locus_tag="WJL87_000039"
     CDS             30916..31107
                     /locus_tag="WJL87_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798539.1"
                     /translation="MNTNQDTIPFGGNLVICCSTGNGGDGRFSCLITDQIPDLNLVRS
                     GQAFPMFIYEYETREKRKA"
     gene            31107..31247
                     /locus_tag="WJL87_000040"
     CDS             31107..31247
                     /locus_tag="WJL87_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEZ7798540.1"
                     /translation="MDTLLKIIIALSFSGAEALAVWLFITAADAVLRRFPKPSHYRRV
                     KK"
     gene            31244..31453
                     /locus_tag="WJL87_000041"
     CDS             31244..31453
                     /locus_tag="WJL87_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEZ7798541.1"
                     /translation="MNINELGARIDRPTIRELIAYATCRNRPISNSTLLRMEKDGRIP
                     CRLKTPLTSPVWDTREVLEALGLQQ"
     gene            complement(31511..32428)
                     /locus_tag="WJL87_000042"
     CDS             complement(31511..32428)
                     /locus_tag="WJL87_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HEZ7798542.1"
                     /translation="MNVSVLNFGNTPVSFRQDGFLNATAIASHFGKLPKDYLKSEQTQ
                     QYISALAENLSVRRKILTEANQIVIVKRGGSEQGTWLHPKLAIHFARWLNPKFAVWCD
                     EQIEILLNGKISDGIKTVTPKPTRALPDGLTGEQIEAVKKLHNALTKSAPKEAQARIA
                     ITLWSAVKSKFGCSYKEVPAEQFPEVLSVMSRVAVENGVLYGEVLDREPLPAPQPALP
                     ISGNALYDLAVAVRYGAWAIQMGRDVSLPLKQLGCKQAVTMWTVWAETRSRLKAAANA
                     LEALNAHADAEHAAKIRPMLPEIRNLSSV"
     gene            complement(33294..33773)
                     /locus_tag="WJL87_000043"
     CDS             complement(33294..33773)
                     /locus_tag="WJL87_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HEZ7798543.1"
                     /translation="MTDNLIQIATPILTVIGVFVAAYGIMRNTENAKKRATIDMIMAE
                     RNNAALQEAITIVNGLAKTDGCILATYTSDTPDKKKDREAILTVLNQREFVCAGVLGG
                     ALHEKMYKDFEYSMLLRDWDNLSSFIFEIRRIRSAPTAFQEFEAVARKWKKKPLKTK"
     gene            complement(34209..34589)
                     /locus_tag="WJL87_000044"
     CDS             complement(34209..34589)
                     /locus_tag="WJL87_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ7798544.1"
                     /translation="MPLKFQPRERSVIMCDFRGYEEPEMVKKRPVVVIARNRHNGKLV
                     TVVPLSSTEPVPLADYHHKMSGNPLPDKPHIQCWAKCDMTATVGLARLDRYKPKGRDR
                     CIPIISEEDFQAIKTAVAKAFKLY"
     gene            complement(34744..35940)
                     /locus_tag="WJL87_000045"
     CDS             complement(34744..35940)
                     /locus_tag="WJL87_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEZ7798545.1"
                     /translation="MAKIITPLSANQVKNAKPRDKLYKLSDGGGLALWVYPTGGRSWK
                     LSFVQDGRQQTISLGRYPDFSLADAREWREEVRRKRAHGENVVNKKVRADFAFEKVAR
                     DWFVRWSKGRSEKYAGQVMRNFERWVFPAIGNLDIRQVRTADVVGCLRVMEARGIVDT
                     LRKTKNSLKMVFAFAVGSGMMEINPVAQIGSGVFERAKTGNMAALSPSELPRLIDFLE
                     QRNEFAVYAGRVRIHPVTRLCIYWLLLTMTRIQEAALMEWSELDGEVWRIPAERKKER
                     RGHDVPLSRAMQWVLDQARALNVNGRFVFESVNFQGHINKESPRVAMQRAGLDTTAHG
                     LRLLARTYLREVLKVDNDVAEKLLAHSLGTRTQTAYNRSELWEERKDALERWGNDVLR
                     LAKNGK"
     gene            complement(36132..36221)
                     /locus_tag="WJL87_000046"
     tRNA            complement(36132..36221)
                     /locus_tag="WJL87_000046"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36185..36187),aa:Ser,seq:cga)
     gene            complement(36299..36389)
                     /locus_tag="WJL87_000047"
     tRNA            complement(36299..36389)
                     /locus_tag="WJL87_000047"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36353..36355),aa:Ser,seq:tga)
     gene            complement(36472..37251)
                     /gene="yaaA"
                     /locus_tag="WJL87_000048"
     CDS             complement(36472..37251)
                     /gene="yaaA"
                     /locus_tag="WJL87_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ7798546.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWNTPFTPENAKQAVFMFNGDVYEGMDANTLNTDQIQ
                     YLQGRVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKTLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHNITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            complement(37513..38700)
                     /gene="dapC"
                     /locus_tag="WJL87_000049"
     CDS             complement(37513..38700)
                     /gene="dapC"
                     /locus_tag="WJL87_000049"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ7798547.1"
                     /translation="MNTLLNQLKPYPFARLREAMQGISAPEGLEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTLNSDTEILPVLGSREA
                     LFSFVQTVLNPVSDGLKPVIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWNDEQHVIDNRRLYQEKFEWVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAEDIVS
                     LYR"
     gene            complement(38776..39228)
                     /gene="dut"
                     /locus_tag="WJL87_000050"
     CDS             complement(38776..39228)
                     /gene="dut"
                     /locus_tag="WJL87_000050"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ7798548.1"
                     /translation="MNIEVEMKVLDERMADVVPVYATKGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVVVPIVQARFKRVEEFVGSSRGEGGFGSTGLH"
     gene            39371..>39784
                     /locus_tag="WJL87_000051"
     CDS             39371..>39784
                     /locus_tag="WJL87_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEZ7798549.1"
                     /translation="MTHISSPRNEFIRGIKESSPMLIGLLPWALILGMQGGQKGMSWL
                     EMLLMTGMNFAGGSEFATVNLWAEPLPILLIATVTFMINSRHILMGAALAPHLKEIPL
                     KKAVPALFFMCDESWAMAFSEIQKRKAAGLPAFNMP"
BASE COUNT         9949 a        10936 c         9183 g         9716 t
ORIGIN      
        1 aaccgacaac tgaccgaaaa aaacctaaaa atagagaaag catccgccgc cgtcattgac
       61 gccaccatta ttcagaccgc cggcagcaaa cagcgtcagg ctatagaagt cgatgaagaa
      121 ggaaaagtca gcggccaaac cacaccgagt aaagataaag atgcccgccg gacaaagaaa
      181 aacggcctct acagactcag ttacaaacaa catacccgta ccgatgagga aggctatatc
      241 gagaaactgc acatcactcc cgccaatacc catgagtgca accacctgtc gcctttgttg
      301 gagggcattg ccgaagatac gaccgtctat gccgacaaag gctacgacag taaggaaaac
      361 cggcaacacc tgaaagaaca tcggttgctg gacggcatta tgcgcaaagc ccagcgtaac
      421 cgtccgctga cggaagcgca aaccaaacgt aaccgatatt tgtcgaaaac ccgttatgtg
      481 gtcgaacaaa gcttcggtac gctgcaccgt aaattccgct acgcccgggc agcctatttc
      541 gggctgatta aagtgagtgc gcaaagccat ctgaaggcga tgtgtttaaa cctgttgaaa
      601 gcagctaaca ggctaagtgt gcctgttgcc gcctaaaagg cggcccggat gcctgattat
      661 ggggcgtccg gggaagatta agggggtatt tgggtaaaat caggaggtat ttggggcgga
      721 aatagctgaa aacctgtgtt tgggtttcgg ctgtcgggag ggaaaggaat tttgcaaagg
      781 tctcaacttg aacaaaaaag aaccgccctg attcagggcg gttatgtttt ctggcggaaa
      841 cggtgggatt cgactaaatt ttatttcatt gatttaaata tatttatttc ttatgaaaat
      901 ttaatttacc ataaaaacag ccatatacaa aaatcttgga gtaactattg cattacacta
      961 ttagaagaat gccgtctgaa gtgttttcgg acggcatttc ccaagctgcc ggaaaaaagc
     1021 taaaatgccc gcaaacagac aaggagcagc gatgcaaaac caaaatacac gccccgtcaa
     1081 aatcgagctt aaaggcgaag caggcaaacg cgtactgctt gccgccgccc gccgcattgc
     1141 caaaacccat caaaaagccg tcaaggcact tgccgacaaa tgatagacgg cgaactggtc
     1201 gcgcttatcc atcaaaccgt attggcggat gaagcgggtt tgaaagggcg ggcggatatg
     1261 gcgtgcttgg acggcgcatt gtcgcggatt gccaactggc ggcagtatga aaaccttgag
     1321 gacatctacg aaatcgccgc cctctatgca caagccatag ccaaagccca cgcctttccc
     1381 gacggcaaca agcgcaccgc gcttttaaca atgttgacct atctggattt gcagggcatc
     1441 agcattgccg ccgaccaagg gcttgacgac ttgattgtca gtttggcggc gggagaaacc
     1501 gacttcaaac agctcgccga aaccctgcgc cggctggata aggaataggc atatccgaca
     1561 acaatgccgt ctgaaattca gacggcattt ttattgaaag gcttttttgc aaccgcttta
     1621 cacaaaggcg gtttttttat gccgtctgaa agcccttcag acggcatttg tttacacggc
     1681 aggagtcccc ccgcctttaa gcaggagagg atgtcaagga tgcctgattt ttaaatcacc
     1741 ccttgaaaga acgggcgcac ggcattaata tacagatatc gacaagcaag gttacaacca
     1801 ttaaggaaat acgatgaaat acaaaaatga gggttttaat ggctggtaag gtgcggaaac
     1861 aaataaaaca aggaggttgg ttgtcagtaa ttgccctaac ctccttgttt gtatctgtat
     1921 tcactttatt ttacatattc aggcacagcg tacagtttaa cctatggggg acggccgaat
     1981 ggctgatgtt ttggcaactg accgttgttt cagtaaccgc cgtcattgca ctggggacaa
     2041 tattcatcaa taagaaaact tcaaagcaaa aggcgacatt agatgttatt ttgaatgatt
     2101 accaagatgc acaatttgta gaagccgaca atcatatttc gccttatatt cgcggcacgg
     2161 cagttgacga caacaacgcg cggatcgacc tgtatgaaat ttatcaaaat aagggcggac
     2221 aatgggaaaa agagagaggg catttactta ccgtaatcaa tcggcacgag ttttatgcgt
     2281 gcgcaatcaa ctcgggagta ttggatgagg atttgtttaa acggctgcat tgcaccaact
     2341 tcataaaatt gtggaatgca gtttcgcctc ttgttatgaa aatacgcgaa gaagaacgca
     2401 aagacacaat atttagagag ttggaaattt tggttgcatt atggaaagca aaccccctaa
     2461 aggcatctga tttgtgaata aacagtcaaa actgttctga aatatgggca gccacgcaat
     2521 cgccgaaata cgccaaagca gccttataaa gtgatttttt gaacataatt ttctccttgc
     2581 ggagcatttt ccaatcaaac agttttagtt tacttggttt tgtatcccta aacaaccgaa
     2641 atccgacatc aagcaattag aaagcttttg catcttgaaa atggataaca aaatattgcc
     2701 tgaaggcgca aatacagtac aaaagccgtc cgaaacagtt cggacggctt ttggcgtatt
     2761 ctgcacaagt atttgaacaa ctgaaatttt atggtaatat gtatctactt tgtagataca
     2821 ataaaggtga agattatgtt gcgtgtccaa aaatggggga actcggcggc cgtccgactg
     2881 cctgccgaca tgctgaaaca attggatttt aaaatcggcg acgctttggt agcggaagta
     2941 cataacggcg aactccgtgt gcgtgctgcc cgacgtttcc gcttggcaga cttgcttgcc
     3001 gaaatggaag aaaccccgcc gcgcgtagaa ggctgggaaa tcttggatga tgtcggcaac
     3061 gaggtcgtct gaaatgtata ttcccgacaa aggcgatatt ttccatttga atttcgaccc
     3121 ttccagcggc aaggaaatca agggcgggcg gtttgcgctg gctctgtctc caaaagcatt
     3181 caaccgcgca acgggattgg tttttgcctg ccccatttca caggggaatg cagcggctgc
     3241 acgaagcagc ggcatgattt caaccttact cggtgcagga acggaaacgc agggcaatgt
     3301 ccactgccac cagctcaaat ctctggactg gcaaatccgc aaggcttctt ttaaagaaac
     3361 tgtacccgat tatgtattgg acgatgtgct ggcgcgcatc ggcgccgtct tattcgatta
     3421 aatgctgaaa ccgcccgaac ctgtaatctt ttcttacagg ttcgggcggt tgcttattcg
     3481 gcacgctgac tgcttactgc atgaccatat gcctgccatt cccttaaatc ggctgcatgt
     3541 ccgtcggctg tttctgccag tcctgcatat ttttcagcgc actgtccgaa taatagccag
     3601 ccttgggaat ctgacgcgcc atcagatgcg gtggcggcgg tagtggttgc gggcaggttt
     3661 ctgttgcgat tttgggcggc ggcgtagtgg cgcaggcggt taagctcaag acgcatagcg
     3721 gcaacagttt tttcagcatt ttgtttttcc tcttctaatt tggtttgtcg ttcggcaaac
     3781 gcggcagcgg ccttctgctc ggcagaacgc gcctgctttg cctgctcgat atagccgtct
     3841 ttcagacgat tcgaaatttc ggcagcagca gcctcccgtc ccatgcggta tttttcggca
     3901 cggtcgaaat gccatgcggt aaaaatcagg acgattagca gcaatacgcc taccggcttc
     3961 cagtatttca ataaaatatc catttcagac gacctcaagg atgcagcccg ggcagataca
     4021 cggttttgcc gtttttcttg gttgcggtca gtatctggtt gcgttgcggg ctgttgcgtc
     4081 ggaaaccgat gtgtacccat gcgccgtccc cgcgctcggg aaattcgagt atcaactggt
     4141 cgaatttcag cgcgccttca tcacgcattt tgattaacag tttggcaaat gccggcgagg
     4201 tcatacccga tgcgtcacaa tcggcggcaa gtccgtgccg gtgcgccgat gtcgggctgc
     4261 caccgaccaa tttgtttacc cgctcgctgc ggaagcagct tgttacgata atcgggcgac
     4321 cgacgtattc gcggattttt tcaagctgct gtgcggtgta ataaatgttg tccatgtcgg
     4381 cgggcgacgg ttcgttcggt acacctgcgc gacgcgcggt ttcactgcgt gtcaactcgc
     4441 gcagactgaa atttttggta acttgcattt cggttctcca tgttcttttc aaataaaaaa
     4501 ccgcctaaac gacaggcggc tatctcaata attctgcgta taaagttttg atacgaaaac
     4561 gccgcgaagc tgcatacttt gcggcgtttt gcttttttca ggcaaactat ttcaaaccat
     4621 gccgatgccg ccatttgaca acccgtaccg aacggctgcc gaaatataca caggcggcaa
     4681 tatccatcat aaatttgcct aagctcggcg tcagtccgta agcaaagact atgtaaaagg
     4741 tatagatcgc tccacctaaa aaggcgagat acgcccacgc ctccggcgac aatgcactcc
     4801 atttgcgccc gttcagacga caaatcatca agtacgccga aatcagtgat ggggcggcag
     4861 aaagaatcaa aagtaatttc attttttcgg tctccacggc agaagattca gcagccactc
     4921 cacaatatcg tctgcatgct tttcgaacga tggccagaca gcgcgggcaa atctcaacaa
     4981 tacctgccac aacaagccga caacgacggg ggcaaaggca tgtgcaaact gtatgcgctg
     5041 ctcacccagc gacggatggc tgccaactac ttcgccgatt aagaaatgcg tcagcggtgg
     5101 ggcaaaagcg ccacccaaaa gcccgccgat aacgatatag gccactacca tccagcggct
     5161 tttcggctcg cttatcatcg ttaccgccgc cgaagccact gcgcccagca cgactgcatc
     5221 gacaggcatc cccaacagat aacctgcgcc gaccacgccg ccaaaaatat aaacggcggc
     5281 aggggtaata atcggttcag acattttctt ttcccttatg cgaaggtcgt ctgaaactgt
     5341 ttttcagccg accttccgtg ttatgaatca gatggctgct tcaggcaaat cggcatagac
     5401 caactccgcg ccggtcaggc tgttggcgga aataggctga ccgtaggcat cgaccgcgcc
     5461 cccttcaacc gctgctacaa cagcctggta tgcccagtcc aatacatccg taccgcgagg
     5521 cggcacgccc ggaatattca actgcatcga accgaccgaa cgtccgcgac gttcaaatgt
     5581 cgatttgcgc acatagctgt ctaacaacac cacattaaag ccggttgcat aatccagcga
     5641 cacatgtcgg atgacgtgaa actcggcaga actgtcggtt tcggtatcga tgatttcacg
     5701 gttgattccg acggtgattt gatttttgtc ttttgacatg atggtttcct ttggataaaa
     5761 aaagaccgtc tgaaccattc agacggtttc ggttttgcag ccgtatctat ttccgtttcc
     5821 gaaaatgacg gtagaaaagg atacaggcta aaaataaagg cagcagaaag cgcgcatagg
     5881 gataccacgg atggtctgca tgccagtatc cgtgccgtcc ctgtggaacg gtatcatagc
     5941 ggtaactgtc ggggaaaaag ttgatccaaa ccgttactgc cagccataag gctatatccc
     6001 tgactgcttt catttacttc tgctcctgtt taaattccca gcaattccat ttcaaagcgc
     6061 gaacgccaac gggattgcgc ggttacgatg cagactttca gacggcggtc ttttgcagtg
     6121 tactttaacc agctttcgta aaatgcctca aggactttac cggcattatt aaccttgctt
     6181 tgcttcaaca ctttaaacac ttcatcacgg gttttacttt tcagatagct tccaaacgac
     6241 gaattcccaa aaataagcgg aatttcgttt ggcgcaagtt tgacaatact tcctgcaata
     6301 ccgtctgcca acaccgccat cggcctgatg cgctcatggc aggtcgcaat ttcgtcaaac
     6361 atcttgtcca ccatatcaac gacggtttcg gtaatttgac tgggctgcac cttgacagct
     6421 ccgtcgaata aagcgttaat aattttaaca taggctgtac gttttgcctg atccataaat
     6481 aaacctccat aaaaaagccc accctcaggc aggcagaaaa aagccgtcct ttcggacggc
     6541 taaaatttaa acaataagac agacaatacc cgataaatcg cccaagggtg aataatcaaa
     6601 attgccgcta atgcggcaga ggatatgcaa cgaatgtgct gcaccatcga tacccttctg
     6661 aaaagatata ctgcctgtct ttggtagatg gaatgggacg gaaaaactaa gaggcacctc
     6721 ctgcggagtc cttatataca gcggcggagg gccaggaacc catacggaaa acttttcgtt
     6781 acgcgctatc ctcttttcag gtgactgaat cgtaacctcc cgctgaatca caccatccat
     6841 gtgcagttgt agactgacaa ctgcaccatt ttctgcaaca gcaaaaaccg gcggcacgat
     6901 aaccgtccgg cgtgtttttc ggctatcagg gatttggcat atcaattcct gataacctaa
     6961 tggcgactgc ttcagctcaa acgtcctgac cgccacgaca tctccgatga tattagcggc
     7021 ctccaaatcc acggcttcaa ttcgcgcgcc acgtatcgta ccacccttaa ttagacctgc
     7081 attgacggtt gcaccattca gcgttggggc ttcgatagtc tggttggcag cgatgtgctt
     7141 tgccagtatc gaaccagtcg cgaacaggtc gccgttcaaa gcctgcttcg ccttgccgtt
     7201 ttcgataacg gtaacgaaag cgggcgtaac cgtcttatct ttcggatcga cataagcaac
     7261 tttatctgcc atcaggagga tttcgccact tcccgtcttt ccatcggctc ccactgcaac
     7321 ccctgcaact gcccgacgac cgcctgctat tgcctcagtc ttcagcgtgt acaaagactg
     7381 tattttccct cctatctctg caacagcaga actggcctgc tgaactttag ccgacaaggt
     7441 agtcaaagca gacgtatcgg cgttactgtc ctccggtgca ggactccagt cagttgctac
     7501 cgtgccgcgc tccagtttta catttgaaac cttgatggat tctgatgtct gataccgcgc
     7561 ctgaacgatg aagcttcgaa gtgctttaac ctctttggca accgtgtgtt tggcgacaat
     7621 acgctgtttc agcgttttgg tcgtaccgct gacggcttcg ccgtaccacg cagcaaaata
     7681 acctaccgag ttgtccgcat agattacgga aaattccgca ccgattcgcg ggtatggttt
     7741 gccatgaggc gatgtggcgt ttaccagttc gatatcgcac gagataatca aactatcgcc
     7801 ttgcttcaga tccaaagcag acgaaacgtc gatagtgacg gttttggtct gattattctc
     7861 gctagcggtc aacactttgc cggctgttcc tgttgataag gcataattcc gaccgccgac
     7921 ttcaacattc tccagcttcg ccgtcaacgt gttgatctca gacgacctgg cagcatcctg
     7981 acgtgttacg gtttcccgca atgccgaaat accgctttct gcattaccaa gtctgacagc
     8041 cagtgtttca cgcgcctgcg cctcggctct gtcgccatcg gtacgggctt ttttttcggc
     8101 ttccagcccg gcggcggtat tgccttgcgc agacgtaaca gtttggattt gctgcgcctg
     8161 ctgctcattg acagcttctg ttgcggcaat acgcgtaccg atttcggatg actttctggc
     8221 aagctcggca gttcgggcag atgattccgc ctgtattgcc gccgttcgcg cccgtgcttc
     8281 ctcctgtaag gctttagcac gttccgctgc ctctgcagac actttcaacc ccgcagccgc
     8341 agccatatca cgattcagcg tgtctatcag ctccctgctt aatgcagact tcccgattgc
     8401 accctgaatc tgctgcacta tcggcgcagg attgttatct gccctaccct gtacggcagc
     8461 ggtaaattcc cccgtatttc ctgcaatatc acgtatgcgc acccagaaat aataaacgtc
     8521 ggcaacgccg acgccggtta aggtataact gttttgcgga tacgccagcg acgcgagttt
     8581 cgttgccgtc tgaagattgt tggtcttgct ataccacagc tcgaaaacca aatcggaaac
     8641 aacggtttgc ggcaataccc aatccacggt cacagacagt gttttcggcg tcgtcctcaa
     8701 tccggtaacg gtatagtcga tactccaagc cttaaccaac ggtgcggaca gtacaccccg
     8761 tgcattacgg ccgcgtattt ccgcccgata attgccgttc ggcagatttt ccaaacgaat
     8821 ttccgctgtc tgtgcatcag ggatatggcg gtataggctg ttgttacggt aaatcttaat
     8881 atcgtaagtt aaaacctgac cgtttgcggc caggttgtcc catgtcacca caactgtccc
     8941 accgtcggta ttgacagccg catcggtcaa cacaggggca gtgttgtgca gcgtggtaat
     9001 ttcatggtca aaatgcgcaa aactatccac ggcagcatat tttttcgggt catgcagcaa
     9061 cgcggtaacg gtataagtac ccgcatccgt attctctttg atgccgatgg cacggtagag
     9121 gcgcggcttg actttgccgg ataaaaccca cgtatcgcca gcctgcaacg gcacctcacg
     9181 ctccaaagta accttattgc ctgctgccgc agttatcttc gctgatttca gactaccgcc
     9241 ttcagaataa aacaccaagg ctccgaccgc atcggataca ggtcgatcca gcgtcagcac
     9301 attgcccgaa atggcggcaa cacgtccaga caactctgca cctgcgtatt gattgtccat
     9361 aatctgcacg atgtcgtaag gcagatgctt caacccctca cgcccgattt caaacgttac
     9421 ggcgttctgc tgccgcaact cagtttgcag cgtccatgcg ccgaaacgtg ccgcctgccc
     9481 gcgcgaatcg cagccgaacg cagtaatctg cttgatattc aaaccatagc gggcaatggc
     9541 ttcgttgtcg gctacatatt ctgttttagc gcgatagcca tcgtatttgt ctatatactg
     9601 cacatgaaca gcagtatgaa tggacttcaa cgctgcaccc gcataattaa acaggccgtc
     9661 tttaacattg gcattggtat attgcgccac cggatcggcg tcggcatcca tgacgacgga
     9721 tacttgctgt ccgttccaaa ccggtaaacc ggtaaagacg ctggcaagat tgttcaaaaa
     9781 ttcacctgcc tgcatcaggt cggtaatgta ggcgttgcag acgaaacgcg gctctttgcc
     9841 gccgaagccg tcatcgaccg gctcgtcgca atatttcccg atttggtaca ggctccattt
     9901 gtctatgtct gccacattca gacgacgtgc cagcgtggaa aatctcggct gggtcagcac
     9961 atcgtaaaaa acccatgccg ggttgtttgt ccatgcggtt ttgaaactgc catcccatgt
    10021 tccacccgta tattggcggg tttcaggatt gtaattggac ggcactttga ccaagcggcc
    10081 tttcatcaaa taattgcgac gcggaatctg attcccgaac tgcgccgaat caatcgacaa
    10141 tgccgcaaac gcggtatgcg gatagctcaa tttcgcatca acgatttcaa cgtaggacga
    10201 aaaataggta ttgttgctga ccttgtcggt cttggaatca ggcgaaattc ggaaaacgcg
    10261 gatattgaac ggcacttgcg gcaccacatc aaattcgaca tcctgataat atgtcccgct
    10321 ggatttttca ttgaaacgca cctgatttga tgcctgcaca ccattgctgt ttatcaactc
    10381 aacgcgcatt aaggtatcag caggcacggt gtcgccgttg tcttcaacct gtgcattccg
    10441 ctcgacagcg accgtaatcc gcaaacggct gaccaattcg tcggtaacgg cgcgaaccat
    10501 cggctgattc tgcttcaccg cagccgaaac ggctacggtg cgctcggaag catcgaaacc
    10561 cggaacatag gactggtctt gctcgccacg ctggaaataa cccgtaactc cattaaaatt
    10621 gtagctgccg tccgaatttt ggacaggagt gtcatcaaaa tacacgcttt tgaacggcgc
    10681 atcatcgccg ttggcaaacc ccgaaaccac accttcggat accgcatcaa taatccgcag
    10741 cgactgggcg ctggataaac tgtttggggc ttcataaggt gtccgttgtc ctccgcccga
    10801 tttgccgccc atattgtttc ctttcctgat tcaaaacctt acttcacggt attcagaata
    10861 ccgatcaaag cccaaaaata cacaaacaaa aacagacacg ccgcgaagtt tgctccttcg
    10921 cggcgtttcg atttttcaga cgacatttca gcccgaaaac gccgccttat aattgcgcgc
    10981 ccgcacgctg tcgtctgaaa agtcggtacg gtatttctgc ccgttcggcg cggttgctgc
    11041 tacgccttga acgaatgtct tggacaaacc caaattcaac accgcacctg acgaagccgc
    11101 cgtcgatgac ggttcgatac ggcgggactg cacaccctgc gatacaacac ggctgccgca
    11161 gtagcaaagc ccgtaagcca acggcacagg gcgaccctgt gccgttgtat tggcaaggtt
    11221 ggaaaaagca gaattcttac tctcctccac ccccttgccg tcggcatcca atttgggctg
    11281 tttggtcaac atttgggcga caccgccagc caccataccg ataccgacgg cgataagctg
    11341 cgcaccactg gcccaactcg tatacgcacc gactacaatc aaaaccactc cgacaatggt
    11401 ttggatcaac ccgccatttt tacctgcacc ctgtacgcgc ggaacaatgt gcaatacacc
    11461 gtcagcaggt cgtctgaaaa cgctacctat cgtttcttcc gactggtctt taccgtcaaa
    11521 ccgaacctga tacaccccgt cccgaatccg ctgacgcaag cctttgattt gggtaaacag
    11581 agcgtgcagg gcttcggctg gcgtctcgac gtgcaaaaca aaacggcggc cgtattcgcg
    11641 aaggccgccg tacagacaaa cagtaatcat gcttgctcca tatgggtaaa atcgttctca
    11701 agtgcctgca tcatggccgg ctcgaaatcg ggatgccgcc aaacgctgtg cgtaacatcc
    11761 gcccaatagc gggtataagg tacacggcag ctcaattgat tgtaggcatg gtgcagcatc
    11821 cagtcgttgc ccaaatacag gacggcatgg gacgcatgcc cgcccgttgc cgtcaagacc
    11881 acatcgccgg cgcaaaggcc gtctgaaaca cgggaaaacc cgcagttttc caaatgcttt
    11941 tcccaaaagc ccgccgctgc gtcctcatcc atatcaccgc gtggatggtc gggaaaaacc
    12001 agccccatca gcatgaaggc atcgcgcacc agcgcgccgc aatccgtctt gccgtattcg
    12061 aatacacgcc cgcgcaaatg cggaacgggg cggaacagtt tcagacggcc ttgtgtaacc
    12121 aaaatccaag gcaagcccgt ttcgacctgc atccatctgt ctgcacccga taaaaacggc
    12181 tcgccgcgcg gatgggaatg cacgactgcc acaatctcgc cgacgcgctc cgccgccatc
    12241 cagtcgtcag cggaaatctc aaaggtttca tgcggatttt cggcgcaatt ggacaaaggc
    12301 agaaactcat agccttcata actgaaaacc aaaaccccgc acatttcaga cggtacggct
    12361 tcttcagcac acgccagcat ttcgcggcgg gcttcttcac ttaacaaatc caaatccatc
    12421 atgtcgttac cttatcacta gatgggaaac cgccgaacgg caaaactgcc gtcgcgccaa
    12481 accgcgccct acatcctgtc agtgttttgc tgcatacgtc tttggacgca tcgtccgtgg
    12541 gcatatcgaa acggtcggca accgcacgcc ccgtataacc gcacccttcg ccgcgatact
    12601 gccagcagca ggtatttgcc agcataatac gggcgggaat gaccgaacca tccgattcgg
    12661 acggcgcggc aagttcgaag gttgccgttt ctgcattcaa tgccgtcatc cgttcaacca
    12721 aatatttgcc gacgacttcc tgcatcgggt ctgcggtcgg attgcgcccg gactggaaat
    12781 tgaccgcatc taaaaacttg gcataggttt gacggcgcac aaccagcacg ccgacgagct
    12841 gaccgaactg atccgccgcg cccgtaatca aacccaagag attcgatacc gtcagcgtcg
    12901 gacggttacc cgcgccttgc gaagttgatt caaagccgtc agccttaatc ggataaggct
    12961 catatgcctg tccctgccag acaaccgccg taccccgctc gttgacctga ttgcagaaat
    13021 agaaaacccg accgcccagc ggccgcaagt ccacttgcca caaatcgacc aaaacatcct
    13081 gctgcgcggc agacaaggct tgcagcatca cacccgacat ggcctttata cgcggattca
    13141 tgaaagcacc tcctcaaacg tacagctcaa ttcgtaggct ttgccaccct tcgccgtttc
    13201 ctaatattcc gataccttga ccaatacagg cacgccgtcg ggcggtctcc aattaaacgg
    13261 ctcaacaccg cgccgggcat caaaaaaggc tttgatttct cccatcaaag ccttttgtcc
    13321 gactatctta atctcccagc ttcgcagctt cggtttcaaa gatttaggct gccgttgctc
    13381 gtagccgtcg ccaaagcgga cggaacggac atcgaattta tgtacagcct tattgtccga
    13441 agcaacacgc catcggaatg tttcagtcat gatcggttcc tttcagacga cctatttatg
    13501 gtaagtaccg ttttcacggt acacattctt gacataccac tgttccacca tgcccggtat
    13561 ggcagcggca agatgcttcg ccatctccgt atcggcactg ctgtccgatt cggtgctacc
    13621 gtcccgattt atagtgatgt tgacctgcat gcttccagcc ccggccgaac ggactccggt
    13681 caaaacggac ggcagtccta ctgcgccgcc accggcataa ccattcaacc gcagccgctc
    13741 gacagccgca acgccgccgt gattacgtac gtcccgctgg gaaaatacga cttcgccctt
    13801 gtgaacaatg ccggcaggct catacttccc gccgtagccg gtatagccgc cgccggaaaa
    13861 caacgcgtcg aactgcgtac ttcctccacc gacaataccg ccgtcggcat aacccaacgc
    13921 cgacttcatt gcattgacaa tcgccatttt aatcagcatt tttgacaaat cctgcaaaat
    13981 ggaaacagtc aggcttcgaa aatcaagttt gcctgtggca acaaagtctg ccagcgcatc
    14041 ccccattttg tcgaaggttt gtacggtggc attttccatc gcctgcctca tcgttccgaa
    14101 tgaatcgatg tagcttttca agccggactc gataccgccg cgccaatcat tgtcgctgtc
    14161 tgcacgcgcc ttctgcattt gagaaagatt gctcatccct tccgccttgc cgcgctccaa
    14221 taaatcgata ctttccaacg gcgcaccttc ttcacgggct ttcgctatct gtctgtccca
    14281 cagtctcgcc agcgtcagtt tctcgatttc ttcacgggtt ttgccgataa gcgaaatttc
    14341 aaacagcctg tcatccaact tttctttcga atcgtcggtc atttgcttca ctatgtcgga
    14401 atattttttg gaggcggccg tcaaactatc ctgcgcatcg gccttctccg ccagctcgcg
    14461 ggcatcggcc tgctgcttca gcgtccaatc gccgaaagtc gggtcggaca acaacttcaa
    14521 ctgttcgccg atggcgcggt tgccgtttaa aatttccagc acccgcttcg ctcccgcctc
    14581 cgccgcccga cggttaaaat cctcgtgcca tttttcgtag ccggaaagag gttctttctc
    14641 cactccctta aacaacccga ggtgcgccat atttttcacc tggccctcaa caccgccggc
    14701 aaatcgcgca gccgcctctc gagaacgcca gttgaaatgc cagtggtcgc cggttgctac
    14761 cgtaccgttt ttattgcgct gtccggcaag ttccagttta aggttaaaat cctgcccgtt
    14821 tttaaacccc aacgactcaa gataacgggt catttgggcg acaacttcct gtttctgctt
    14881 catccccata ccgggagaag gagtggcatc aaatgccaat ccactgttat gcaggctgtt
    14941 tttcccaatg tggtatcgat ctcggaaggc gccgaaacgg aacagacggt ctcccagcat
    15001 ctgctgcatg gcatgcgcga aggcatatgt tccgcctagc gctttgccgc ccgcttcggc
    15061 tcccggttta atcctcaagc cgttcgaaac agtggggtag cttgttttat ccgttttttc
    15121 tttctgtcgt tcgtgttttt tctcggcaaa actttcatta atcagcttgg catttgccgc
    15181 gtgcttatta atgatttctt gccgcttttt cgcgtcagtg atgccgtcca acgccttttt
    15241 ccgtgctttg tcgtttttaa gcagttcgcg ctgccgttgc tgctctcgag tggcataacg
    15301 ttccgaaaca gcctccaatt cggctatacc ttcagttcga ttttggttct gcttctgttt
    15361 ctgccgcaaa gtttcagcag cggctgcctc cttgtcgatt tcggcacgca accgggcacg
    15421 ccgcgcatag gcttcctgca gcttacgctc accctcttca ccccaaaaca attgtgtaac
    15481 cggatgtttc ttgctggttt cgatttggga aataaacgaa tctagcgcgg caatttcatc
    15541 cagcttggtc ttgtccctac ctatggattt aacagcatcc catgcctccg acgcactttc
    15601 tttaatatcc ttccacgccc gctcaatcaa cccaagatta cccagtacgc gttttgccat
    15661 atcctccgac tcttcggaga atttccgctg caccaatgca accgcgtcct gttcccgacc
    15721 ttgtgcctgc aaagccttga cctgctggta aacatcggca gtcattgtcc ggtaggtggc
    15781 tgatagggat accacagctt tcagcgggtc tttggcgatt tcagtatatt tctcaaccaa
    15841 atcgtcaata ctttgccccg ttgcctgcga ctggagggta atactccggg cgaactgttc
    15901 gtaattgtcc gttgcaacct tgccgcttga gaccaaggcc gtaattgcag cacgcgcatc
    15961 agcatagccg cccgtcgctg ccccgaccga aacagcgatt tcctgcatcc tgtctgcagt
    16021 aatccctgct gcatctccgg caagtatcaa ggctttacgg tattcgcacg attcctctgc
    16081 cccctgatac atcgcataac ccaatgccgc aatgccgccg cccaaccccg cgacagcaag
    16141 gcgcataggg gaaatgaccg tggccagtcc cttaagcata ccaccgaagc cgccatacat
    16201 atcacgcaac tgcccaccct gttgaatcat gatcagaaac gggctttgcc cgcctgcaag
    16261 ctgggtaacg atgtcggtaa tttgtgccgg ggtttgccgc aatgcgttat tgaactgctt
    16321 gacagactgc gtagcgcgat tggtctgcgt attatgcctg tccaattggg acagtagcgg
    16381 attaagccgt gcaatatcga taccgcgctg gcgcgcaagc tcttcgtaat actctcggtt
    16441 tgccttaccg cctgcaatct taacggctat atcgcggcgg atggcattct ccagagaagc
    16501 caaagaccgt tccgtccgct tcgatgccgc cgacactacc gtcgatgatt tctctgcgcc
    16561 gtcgccacta tgtaccaatc cgtcagaaat acgccgcccc tgggaagatg ccacatcccc
    16621 caaagacttg atggattgtt ttgccttttc tacgccgctg acgacaccgc cagtttcggc
    16681 ggtaatataa atcttggttt cgttattcat gttcgcctgc ccacattgac aacacttcaa
    16741 gctccatcag gcggactttc tccaacagtg ctttccgttt cttcgacttg atattgccgc
    16801 attccataac tgctgcgact gcggaataat ccaaccccgt cgcacccgac attccgatac
    16861 gccactgcgt cgatactcca ataaacagtt gcaccgcctg ccagttgcac ggccatacgc
    16921 caaaagtggt ctccttttct gaaaaatcgt ccgccgaaaa tccgaataca ttcaattccg
    16981 ccgcgtcagg ctgcttctca taaagcgcac gcgcggcggc aatcagtttc cctcgcgggc
    17041 attgaagact tcctcaacat agccgttgac gatggcaaac accgccatcg gatagctatc
    17101 cagcaggatt tcgacattct gccggtcgaa tgcttcttcc aaatcccagc cttgaacaca
    17161 atctaagacg gtatctgccg tccagccgtc tttatgcttt tcggtaaatt ctttcattgc
    17221 cttgcggccg cgcgccttaa attcaaattc gacgtccgca ggttctgcac cgggaacagg
    17281 gatttttacg gtatgtcgga aagttgcatc aggcttcaaa gtgagttttg ccatttcaat
    17341 actccaaata aataaggttg cccgagtaac tcggacaacc ttgatatgcc gataaaatca
    17401 ataacggttt acttcgccgg aaatggcgta ggacaacgtt accgccatta cttggttacg
    17461 aaccaattcg ggggttttat tcatgctggg ataaccgtta tagcaaatca gcccaccacc
    17521 tttcaaaacc actttcaaag gccacttgcc gcctttatcg ctgcattttg cagccgcctt
    17581 gtagccggac aacgaagtat cgtcagcgat cttaatcgac attgacatag ctgatttcgt
    17641 ggatgggatt tgctggtcaa aatcgtcctc caggaaacca aaatccacat attgctgctc
    17701 gccgccactg gtcgaaaatt ctacaatttg cgaaacttgc acccaatcgg taattttctg
    17761 aacagcacca ataccgctgc ctgccggaaa ctcatctgca ttggaagtat caatgccggt
    17821 cagcttgaat gtatcggctt tgacatcgcc aatctggaaa acgcgttcat tcaactttcc
    17881 ccagccgctt ttaaacaaaa ccaaatcgcc gttggcaaac ccgtgtcctg ctaccgtcag
    17941 gacgcattcg gcagcattag ttgccaccgt agcttttttc tccgccgcca aagcggtcgc
    18001 aatgtgaacg gtcgcaccgt tcggtaattt cactgccata ctttaactcc aaattaaagg
    18061 caatctgaaa tcagactgcc gtatcaaaat aaattgcctg ccatccttgc tgataataga
    18121 cggtaacggc ctcgccgctt tcgtcggaat cgatattcag caaatcaggc accagatttt
    18181 ccaaatcacc aacacccaaa tcggaatgtt cggcaaacaa ctgcccaatc aaagacagca
    18241 tctcttccgc cagttcgtcc gcaccggaag cagcctgcac gcaaaccaac gtaaccagcc
    18301 gcaccgtatg ccggtaggta ggcggaaaat caaacgtttc ctgttctgtt cggcgacggt
    18361 caacgtacac cacaacacac ggcaactgcg attgcgcagg cgcgaaacca cgcccggaat
    18421 acacgcgctg gaacttcgtt ttcagcacat ctgtcgccgc attgcggatt tcagtaagtc
    18481 ggcttgtcat gtatcgccct caattgcaca acaaccattc cgcagccgtc aaaatcggtt
    18541 tccgccaccg cataacgttt gccacgcacg gtaacaacca ccgacttcac gtcttccggc
    18601 aggtcgtctt cagtaacgat gatttgcggg tcggcattgg caacggcaac gccaaacccg
    18661 ctatccgtta taaattcacg gtcaaatatc gcgtttacct ctttaccgcc aatcatcacg
    18721 gtttcaccga aatcggcagg attggtaaac acgttcagcg gctcgttaaa cactgccgtc
    18781 ctcgccgccc tcaccggatg cgttaccgct atcttcagct tcgccgccat cggcaacagg
    18841 ggattcggcc tctttggcag gcgcttctgc cgcccaacct tcgcggatat agacagccgc
    18901 cgcagattcg tccaccgaat attttttccc tgtggcaaac gtctgttcac catcgaaaaa
    18961 actacgggtc gggataattt tgatttttgc catttttaat acctcaatta ataaggtcgt
    19021 ctgaaattca gacgaccttg ggtttcacaa tcaagcagcc gcgatgtctt taatggcggc
    19081 aaaggattcg acatgacgga cggcaatatc cacatcttgc aacgtggtga tgcgtaccgc
    19141 gcccgtagta gacttggtgt acggatcaac aatcacatcc aaaacgcccc agtgcgcaat
    19201 catcaaatca gaccagttac caaaaatcag cgggctgcat ttgttggccg ccgtgccttt
    19261 ggtcaggttg gacggaattt ggtttgatac cgcgcaacgg tagccgttca acggcgttgc
    19321 gccgtcctgc tagatgtagc cggacacgcc gtcggccttc agcttggttt tcagcagacc
    19381 gcggacgcgg gcattggtga tgtatgccaa atcgccgatg tcggcattgg cggcggcaat
    19441 ggcactttcc aaagcgacga tatgcttcca ttcgggcgca ccgccgttcg caccgatttc
    19501 caccgcgccg atgccggcgg tattcaggat gccggtcggt tcgttgccgg tacctttgcc
    19561 gttgatggcc gccaaatcga tacccagcat catcgctttc aataattcgc tgcgggcgaa
    19621 ctgttcggca gacagcgagg attgcaaaat gaatttgcgg ctcaattcag tattggcggt
    19681 aacggttttc ggtttcaggc tcatttgtcc gaaagtggca ttcgattcgg tcgcgctgcc
    19741 gttttcatcc acccattgaa cggtgtttcc ggtcaggtgt ttcggaatag tgatgtcgcc
    19801 gaccaagccg tccagtacgg tggcgcccaa ctgggcaacg gcaagtcggt tgcgcagcag
    19861 ttcgatgaac aagtcttcgc ggaagtcgtt ttcgatgacg ttgccgccgt ttgccgcatt
    19921 gcctttgctg taagcgcggg caatcaaatc agtcggcaca aagaagcctg ccgctgcgcg
    19981 accatgtcgt ttttccaact cttccgacac ttcgcgttcc aagcccgctt tgtcccattt
    20041 gccggttgcg gcggcagaca tggcgcggag aagggaaaat tcacgctgtt ctttatcagt
    20101 catgccgatt tcaccggcgg taacggtcgg cttcgtctgc atgtttgcca tgatggcggc
    20161 gcgtaattgt gcttcactgc cgccctcttt aataaccttt tcggctgctt cgataccgcc
    20221 gtgggcggcg taactgcgac caatggccag cagttcggaa acgcgcgcgc gttcgttctg
    20281 cataccgcgt tcggcggtgt tgttggtatc ggttgctgcg gcggcaggga ttgcagcggc
    20341 ggaagtttcc accgtagtgg gcgtttgatt tttatccatg tttcgatttc ctttttcagg
    20401 ggttgggttt acagggtttt caataaatgg ttccaccgac ctgcctacac ctactgtcgg
    20461 gtctgcagga acggttacaa aactgatttc atacggcatc cagcgggtaa cgatgtaacg
    20521 gtagccctca tcgtcagtat cgggattgtc caataccata tcctccactc ggtagccgac
    20581 gctgatatgg cgtaggatgc cgtcttgcac gtcttggaat ttttccgttg ccttcgtacc
    20641 gttgccaaaa cgtaccaagg cacggccgcg cttgtcggca tcaatccaag cacgttcgat
    20701 gatgccgatt tggtcgtccc agtcatgatt gaacaacacc gcgccgccgt cgttcaggcg
    20761 gcttaagtca acagcaccct gcgcatgact taatacttca tcaccgaacc aacggcgtac
    20821 cgattcttcg ctggaaaacg ccacttcgac cgtccgtttt tcaacatcaa cactttcgcg
    20881 ctgaaatacg gcaaagcggc tcatttgctg catttgcgcc ttatcaggct tcattttctt
    20941 cattcgggtt atcctccgat tccggttctg ccgccgatgc ggccggtttt acgtcggcaa
    21001 tgattccttt tcctgccaac atctggtttt cctgttcgat ttgggtaata acatcttcaa
    21061 aatccaaacc catttccgcg caaatatcgc ggcgggattt gactgccagc gccaccgcct
    21121 ctttatgcgc attaatatct ttcagcgggt caacccacga ccagcggcgc ccctgccagt
    21181 tgcaggcctt gaacttgtcc agcttgccgg caggcagcga tttgccggac ggcatcttga
    21241 ttgcgcccat tagcaacgcc gcctcaatcc agcgatcgaa tacgtcatac aagaacgcgt
    21301 cggcaaacca gttttgcaac gtcatccatg tgtcgcgttc ctccagcgtc ccgctgcgga
    21361 tactggaaaa gttcacgccc tcaaggtcgt tcgccaagct gtgataagcc acgttcaaac
    21421 cgctggcgat accgcgcagg ctggctttga cgaatgcgtc gtaattggcg tgcgggtaat
    21481 ccggatcaaa cggcgtaaag tcgtaaccct gcggcagttc gtgaaacgta cccggctcga
    21541 ccgaatcgat taaatcgatg ctgccgcgcc cgttatcgac ctcttgcccg tcaatcggcg
    21601 gcatgaagtt gtcggcgtct tccgtctgtt tgaaaaagcc cattttcgat gcaccgacac
    21661 gggcagcgat aatggccgct tcctgatagc ccgacaggtt ttgcaggccg atgatggccg
    21721 aagccaccca cggaaagccg cgccgctgct ctgggcggtc atgcaggaaa atatggctga
    21781 tttgctctgc cggcacccgt tcgcgtaaat tgcccgtatt cgtctgtccg tatgattcgc
    21841 ccggatgtgc cgtccgcaac cagtacgcta caggacggga atagctgttt aactcaacac
    21901 ccatgcgcac ggcgttacgc ccgttttgct gcggaacgtt gtatcccgta tccaaacggt
    21961 caatatccag cacctgcaac gcatagccgt aatcattgtc aaaccctgaa atatgacgaa
    22021 tcagcacttc gccgtcccgc gccacgctgc gaatcagcag ccgttgcagg tcggtaaaag
    22081 acatctggcc ggtaacgtcg cacacgccgc gccgcgccca gcgggaaaac gctgcttcaa
    22141 tggccttgtt cgccaaggca tcgggcttgt cggcgttatc cagcaaaaca cgcatttgca
    22201 gggcaaaacc gtcgcgcccg ataacgttgc tttcgaccat attcaaaaac ttgcgcatgt
    22261 agtcgttgtc gcgtgcaagg ctgcgggcgc gagtgcgcag gcggtcaagg tctgaccgcg
    22321 ccaaagcgtc cgccgaccag ttttgcggct gccacgaagc cagcgacccg accggacgcg
    22381 cccctgcaaa gttgcggcgg gctgttttcg ggacggcttc ccgcctgccg aacaaacgcg
    22441 caaaaaaacc gcgctttttc ggcgcggcat ttgattgttt tgccatagct catcctaaaa
    22501 tcgcgtgatg atacggcctg aacggcgcgg ggaaacaccc gccgccgctt ccagccgctt
    22561 gatttcctgc tgccagaacc gtatctgctt caacaaatcg gcaaggctgg aaaactccat
    22621 ttcgcggtct ttgattttgt accgcttggt catgcccctg cctgccgcat gttccttgta
    22681 ggcttttcga aggctgttca gaatctctcg cgcttcttca agttctaccg catagtcatg
    22741 tccgctcatc gccgtatccc ttcaacagtt cggaaacacc gcacattgcc gcaagcagac
    22801 tgtcttcaga ttcaaaaaat gcatgataga gaatttcttc cagtacatgc tgcatcaggt
    22861 catccaattc gcatttttcc agaccgtcca ttaactccgc atattcttcg ggttccaaca
    22921 gatgcattac ggcacaagaa tcaagctgcc gctgcaaata ccatcgcgcc tttttcaaat
    22981 cctccgcgcc gttcttttcc cgatgacgcc agacatactt aaacgcatta cccatgcaga
    23041 aattcagccg ttcggtaaat tcaatgcatt ccacggcgcg gcttcgatag tgcggcgggg
    23101 tgatgttgtc ttgcttttcc attttcagac ggcctctttt gcataaaatt tactgacgcc
    23161 ttgctcaaaa cgctcttcca ccaagccttc ctcaatcagc gtatcaatat cgcggcgggt
    23221 taaaaaatgg cacagagcat ggcacaatgg cacaaattta tggcacaacc aaaaatccaa
    23281 actacaaaac gcgcccgaca actctgccgc acgcgttttg aaaacatcat acatatgtca
    23341 gactcaatcg tcaaacttct tcaccgccgc gatacctcca accagcgctg cggaatcgta
    23401 aaacctcatt gcccagggca tccatttccg gcaatccgtc cgccgcttgc cagccagccg
    23461 gcttaaaaat ccagttttga cacgatacgt cgccatcttt atttttcgtc cgccaccagc
    23521 gccgcgcccg cggcatcttc tcagcaatgt gttggagaaa ctttgtttta ccagtttccc
    23581 gctcgccgtt ttgcctgcac cactctttgt aaaccaacca aaggtcgtct gtaatgcaac
    23641 agcaaaacgg caggtcttta ataccaccgc cagcccactc cgaataaaag gtttgccaag
    23701 catatcggcc ataggaaatg acgttttgtt tcgcggcagt catcggcggc ggcgtgtttg
    23761 gatcgaatct tacaggctct tccagtttga ttacctcgta tcctttttcg ttttctgctc
    23821 cctgttgccc gtaagtcaga taaaaattcc aacacagcag gaaactgtaa aacaaacgga
    23881 caccatcgtt gccgatacag tctaaaactt catcgcgcaa ttttttgtcc aactttttct
    23941 caggccatac aacgaaataa cgccggtcgt tttcctcaat tttgaaagga tgagattcat
    24001 tacgcaaaaa cacacaattc atatggtttg cttcttcgta agcatcctgg aatttccgct
    24061 cgacgcgcat cgtcttgccg gtaatcatat gtttttggct gcctgtttta ttgtattttt
    24121 gctgatttgt gtaaacctct tcaaaaatac cgaacatttt gcctgatcgg tttgccgtat
    24181 agttgctttc caagtcctct tgaccatatg ttgcgccgta ttcgccgtac atcggcttca
    24241 cgatttcctc gaaaaagaaa gatttacccg caccgtgtat atgaccgtgc atgacaacgg
    24301 cggtcgtcat cttcgcgcct acgttttgta acggataagc cagccaattc aaaatgtatt
    24361 cgacctgttg gtaatcccca ttacataagt gattaatcaa attcagaatc ggcttgattt
    24421 cagggaataa tctatacaat tcgtagtttg tttcgggcat atcctctttg ggaatcggga
    24481 atttcggttc aatcggcatg cctcggaaga tgttgatata gtcgtcagac aacacccgac
    24541 caggctcaaa gaccaatcgg tcgaaccgtt ttacaattcg gctcgggcta ttgacccaaa
    24601 ttttaaaacc atcaggcgtc atcgacagac gagccgcctt ttcagggaca acgcgccata
    24661 gtttgttgtc ccaaatcgat gccgaaccat caagataaat gaaacgctcc atcatatctt
    24721 gcgtgtcatc atcttttacc gcctgaatca atgcgtagtc ttttttcaga cggttgattt
    24781 cgtacatagt cagcaccggc gcacggcccc attcatacca agcatcgaca acgtctttgc
    24841 cgaaatgaac agtcattgcc tttcgggtat atgtaacccc tgtttttctg ttcacgccgt
    24901 ggtctttacc ttcaaccagc ccaaattctg atttcagctt ttgcaatttg gccaaatatt
    24961 cgtcatccac atcccccgca cccccttgat tgcctgttgc ttcgagagat tcatgcaact
    25021 gctcagattc cggctccata tcttgaatgg gtgcgggaga gttttcagac ggcatttccg
    25081 cttccggcac atactcaacc aaccaatccg ccgcgcccat ccagctcaac acttcggaag
    25141 ggtcgcagat gcgcccgccg tctgcaatcg catcggcaat atcgaaacca tgaggccata
    25201 tccctggagc aggaatattg accaaccaaa cacggcaatt ctgctttgtc agtacctcag
    25261 caataccaag catcgccttc attccgccct gctcattgcg cggcagaaac ggtttggatt
    25321 cagggtcaac accatcatcg cgatctttct ttgttagctt ttggcgcagc gaatcgcaat
    25381 caggccacaa gacgacatca cgatcaacga ccgccgacca atccgactta tcccagttat
    25441 tacaaccgcc atgccacgtc aggacggcat agttataacc ctgagcatct gccgcgttct
    25501 tacatttctc cccctcgaca accaaaaccg gtaatgatgg gtcagcaacc aaagaatcaa
    25561 gaccgtacaa tgggcgcggg ttatcccaac aacgccaacg ccacatcttt tcgcctgttt
    25621 tcttatgctc gcaaaacgta taaggcagat cgatttttcc gccgtctgat tttctaaatc
    25681 gggcaacacc gcccaaaacc gcgctggagc tatcgcggaa aacagattta aaaatcaggt
    25741 cgtctgattt tctaaatcca ttcccaaagc tcatagaatt cagcgcatat tcaggcacag
    25801 gaacgattgg cttccacaaa tcacgctcct cctcctttac tggagcagga ggaggtaaca
    25861 gcttcggagc cttatctacc aaatccccat gcgccaaccc aagcaaggag gcaactttgt
    25921 ctctagcctc gttaaaacta tacccgcaaa catccatcaa caaatcaaac ggactgccgc
    25981 cttccggctt gcattgattg caaatccaaa caccactccc gcgacttgaa ccgctaccgt
    26041 ccgtaaacct gaatcggtcg gagccgccgc atgacggaca ggggtgatgt tttttatcta
    26101 aaatttcgga cggtatgccg cacgcttcta aaacctcacg ccaccggtca tgcgcggcgg
    26161 cattaatttc ggcttttgtt gcatattttt tattcatttt cagtcatcct ttacgttttt
    26221 taggcgtaaa aaatacccac ccacgcacac aagaatcact ctggagaggg cgggctaaac
    26281 actgattgcg cgattaatca gtacagggta attaaatctg taacatccct tgagggataa
    26341 ataacggctc agtccacaag ccgatgtgca gtatcggaca ggctgctttc gccaacacga
    26401 ggctttgtgc gatgcttcgc gccttagctg cctcctcttt ttcgcgctcg gcgtccatca
    26461 atgccgccat gactgcctgc ttttgctcaa attctttttc acggcgcttg tacagcaaat
    26521 cttgctgata gacgttgtgg catcggcggc atcggggcgc gtatttttgc agcccggttt
    26581 ttctgtcaaa gccgtttttc ggaaacgccg ttatcggctt aatctctccg cactccttac
    26641 acgtttttgt tcgattcatc gagaactcca atattaaaaa ttatcgtcat caatgcggct
    26701 tccccagccc acagcgagtt ttccacgaca ggcttttttt atttgggttg gtcgtctggt
    26761 ttctttgagt tcaaaaacag gtcgggatac ttaagtttta ttcgtgcagg tatcccgcgc
    26821 ttcgtccaat tgaaaacgca ttgagggcta ttccctgtta ttcgaccaac ttccgcgtaa
    26881 ctgccgattg attgcaacag gcgtttgtct tcattgactc ttttatccat aaataaacca
    26941 aatgtttaaa tctaatgcta atattaaaca ctatgtttag ataaaaatca agtcttgtgt
    27001 aacaacattt tgtttaaata tgggagaata attaagccaa ccgcgaatag gattaaaaat
    27061 gacaatgcac gagacaactg acagactttt tgagatagcc aaagagcagg gagttttaaa
    27121 gccggctgac atagcagagc gtctgaatat cagccaacag gctttgaaaa actgggaaag
    27181 tcgtggcata gcggcaaagg cgctgcctga agtagcaaaa gcattcggtg tatctgaaac
    27241 atggctgaga acaggtgaag gcagccgaac cgcgcccgtc cttattgacc ccgacctacc
    27301 ccacgaagtc aaagacatcc accgcccgat gacgtggagc agcaacgacc cgctgcccga
    27361 cgatgattat gttttcgtcc cctacctcaa agagagctgc ttcaaaggcg gagtaggcac
    27421 gtatgaaatc cccgattaca acggctaccg cctgccgttc ggcaaatcca cgcttaaacg
    27481 caaaggcatc aatcccgaca acgtgttttg ctgcaccctg accggcgaca gtatggagga
    27541 aaaaatcgca gaagacgcgg caattgccgt agatacgggc gaaaccgcca tacgcgacgg
    27601 caaaatctac gccttcgccc aggacggtat gttccgcgtg aagtacctga tacggcagcc
    27661 cggcaacagc gttctgatac gcagccacaa cagcggtttc tatggcgacg aaaacgcccc
    27721 cttggacagc ctgaccgtta tcggcagggt attttggtgg agcgtgctgg attgaaaaat
    27781 gttgctatta cgacgagata atattgatag ggctttcaag attgtcaaaa ataggcgttt
    27841 tgattcccct tggtggcctg gtgagtacga tgccggtatg aatttccttg gcgtacaagg
    27901 agagttgaag gttcacgagc tgcatcacag aacagcaacc ctgtgctttg agtggcttgg
    27961 cgaagtatct gcgccgcgca gaaaagagaa ctacaaagac ctcaagccaa atgtattgta
    28021 tgactttgat ggttccggta agcattttgc caaccctgac gcaaggtatc tattgcccgt
    28081 cggttcaagc ggcttaatct taaaacatat ccaaatcgat gacgaagaca ctctgttgcg
    28141 gctatggtgc gcacgaaata tcccaatgcc acaccggcta tctaaaatcc cgatgcttcg
    28201 gcagtattat ctaagcaagg catggcacga aatttatgct atcaaccaac acctaaggaa
    28261 aacgaagctg atagttgatg tggcatacgg ccccacagat taaacaaccc gcccgcatta
    28321 tgcgggcttt tttcatgccc cgccgaacct gaaaacaaca caaaaccgac aaagccgcct
    28381 taataggcgg tttttttgcg cctgcgaaat ctcaaaaata aattctttta ataatcaaaa
    28441 tgtttataaa tcttaaacag attgttgcat attattaaac gctatgttta aatacaccca
    28501 tcgaaacaaa caacagccac atggagtacc gacatggaaa ccatcaccat ccctaaagaa
    28561 aatttcgacg ctttgctaaa tctcgcaaga accgccgtcg ccctgaacga aaattttatt
    28621 ccaaaaataa attacggagc atccaccata ccagcagccg gctttgccgc acttaacgag
    28681 tttggaatag ctcgctccac cgtggaatct ttggaaattc tcaaaattaa ataacctcct
    28741 cggtattgtc atcccgtgat accggcaacc taactaaccc ctctttaaag gaaaatcaaa
    28801 aatggaaacc caagaaaaga aatttgttcc tgcctccaag caaataccga ccgtaaatag
    28861 cggctggtta gaccgtgaat taggcgaagc catgagtgaa gcggtgcgtg cctgccttgc
    28921 tcacggtaaa cagtcagaaa ttactgtcaa gctcaaaatc caaccccaaa acatccaaag
    28981 cggaacggta aaaatcagcc acgatgtagc aaccaaactg cccaaagaaa aacgcgaagg
    29041 cggcatcgtc tttgctacac ctgacggcaa catccaagcc gacgatccgg cacaaggaaa
    29101 attgaaactc aaacaagttc ccaatacgtc aagcacgttg aaaatggtca gatccaacta
    29161 actcaaaccc aaaaggaaat ctaaaatgga aacccaagaa aacatgatta aaaccgcctt
    29221 acaagcagct caaaaacctt tttttgagtt tgcaccgaac aatactctgc ttgtattcac
    29281 accagaccaa gacggtggct ggagatacaa atcacacccc gaattgatgc aaaacccgta
    29341 ccgcaagtgc ggcaaattcc tcatgcacga taccgccagt cttattaagt ttgtacaaaa
    29401 acacaaacag gacggcacac aaatctacat tgatgccgat ttcaaatcag ggcgtattga
    29461 tgtcaccgcc gtcatcaacg gccatacgca acaagctcct ggctggctcg atttttatgc
    29521 cgtatacacc ccgcaacaca ctacatcggc atccaactgg ctgaacaaca atgcgcaccg
    29581 catgaatcag atggagtttt cccatttcct gaccaacaat gcccgcaaca tcgtatccaa
    29641 aaatcccggt aacgaaaatt ccgtttaccc gaccgccgcc gaagtattgg atttcgcgct
    29701 caatctcgag tacaccgaaa aaaccacctt caaacaaggc tatcgtgaac aggacggcag
    29761 aatcaacttt accttccaat ccgaagactc agggcaaaca gagaaaaacc tcaaaatgtt
    29821 tgaacgtttc ggcatcgagt tcaccccgca tcaaggcggt gcatcctact ttgttgaagc
    29881 cctgttgaaa ttccgcatcg acaaaaacag cggcgcactc gtcctgtggt acgaattgca
    29941 gcaaatcgat gctgtaatcg aacaagcggc aaaagacatt tccgaagccg tacaaaaagc
    30001 cttccctgaa atcgacatct atttcggcat aatcgcctaa aaaaaaaggt cgtctgaaat
    30061 tgtgaacctt cagacgatct ccacaaaaac ccaactccga aagaccccaa aatgaccatc
    30121 acagaagaat actccatcga catccgcgta accgcagaac aggggaaaaa cgactacggc
    30181 taccccaccg aacgctacgg atgcgacatc atcaacacag acggggagct gcttgtcggc
    30241 atagagccag agtacaaaac cccctttgcc gccgtcagaa aggcgctcat ctgtctggca
    30301 aaagacaacc tcaaaaacgc tgcctgatat taagccgtta cccccaataa cgaggaatca
    30361 aaatgaaata cttgatccgc accgccttac tcgcagtcgt agccgccggc atctacgcct
    30421 gccaaccgca atcagaagcc gcagtgcaag tcaaggctga aaacagcctg accgctatgc
    30481 gcttagccgt cgccgacaaa caggcagaga ttgacgggtt gaacgcccaa atcgacgccg
    30541 aaatcagaca acgcgaagcc gaagaattga aagactaccg atggatacac ggcgacgcgg
    30601 aagtgccgga gctggaaaaa tgaactttca ggaagaaaac gagaacatca agaatgacat
    30661 agagattaat ctcttgcatt ccgctgaagc cgacagcgtg gaggcaatga tggacttggc
    30721 ggtttacggg ctggcggcga tggttgccaa acacaacaaa acgcgcggcg agcagcttgt
    30781 gttacaggcg attgccggtc atatgcggaa gcttttggcc gcgcttcccg aaaccgagtc
    30841 attggtgcag accgggaagg tttaccgccg acttgaagat ttgattttta aaacgtattt
    30901 gccggagcag taaagatgaa cacgaatcaa gatacgattc catttggcgg caaccttgtg
    30961 atttgctgtt cgacgggaaa cggcggggac gggcggtttt cttgtctgat taccgatcag
    31021 ataccggatt taaatcttgt cagaagcggg caggcgttcc cgatgttcat ttatgagtac
    31081 gaaactagag aaaaaaggaa agcctgatgg acaccctatt aaaaatcatc atcgcgctgt
    31141 cattttccgg cgctgaggca ttggcggtat ggcttttcat cacggccgcc gatgccgtat
    31201 tacgccgctt cccgaaaccg agtcattaca ggagagtaaa aaaatgaaca tcaacgaact
    31261 gggcgcaagg atagaccgcc cgaccatccg cgaactgatt gcctacgcaa cctgccgcaa
    31321 ccgccccatt tcaaactcga cactgctgcg tatggaaaaa gacggacgga taccgtgccg
    31381 tctgaaaacc ccactcacat cccccgtatg ggacacccgc gaagttttgg aagccctggg
    31441 gttacagcaa taaaaaaacg cccgattagg gcgttttcac attcagacgg cttttgtatt
    31501 ccctactgca tcaaaccgac gacaggttgc ggatttcggg cagcatcggg cggatttttg
    31561 ccgcgtgttc cgcgtcggcg tgtgcgttta aggcttcgag ggcgtttgcg gcggctttga
    31621 ggcggctgcg tgtttccgcc cagaccgtcc acatcgttac cgcctgtttg cagccgagct
    31681 gcttcagcgg cagggaaacg tctctgccca tttggatggc ccacgcgccg tatctgacgg
    31741 caacggcgag gtcgtacagg gcgttgccgc tgatgggcag ggcaggttgc ggtgcgggta
    31801 acggttcgcg gtcgaggact tcgccgtaca gcacgccgtt ttcaactgcc acgcggctca
    31861 tcacgcttaa aacttcgggg aactgttcgg caggtacttc tttgtagctg catccgaact
    31921 tgcttttgac ggcagaccaa agggtaatgg cgatacgcgc ctgcgcttct ttgggtgcgg
    31981 atttggtcag ggcgttgtgc agttttttga cggcttcgat ttgttcgccg gtcaagccgt
    32041 ccggaagggc gcgggtgggt ttgggggtaa ctgtttttat gccgtctgaa attttgccgt
    32101 taagtaaaat ttcaatctgc tcatcgcacc aaaccgcaaa tttcggatta agccaacggg
    32161 caaagtgaat agcgagtttg ggatgcagcc atgtgccttg ctcactgcca ccacgcttca
    32221 cgataactat ttgatttgct tccgttagga tttttctcct aacgcttaaa ttctcagcaa
    32281 gtgcagagat atattgttga gtttgttcac tttttaggta gtctttaggt aacttgccaa
    32341 agtgagatgc aatggcggtt gcatttaaaa aaccgtcttg acggaaagat acaggggtgt
    32401 taccaaaatt gagaacagat acgttcatga tagtttccta gtttagtttc gaaagaccca
    32461 aatgggtggt cgggaggttc gaaaacctac tagaaaaggc cggcgttatt ccccttgcgg
    32521 gtattgtatt ccgcaccctc ccgaccatag gaaactagtt cggatatatc gaaacaaaca
    32581 accaaacaag aaaacttatg gacgtaaaaa attcacgctg acggggtgaa tgccgttcta
    32641 gtagaggttt tcgacgcctc gtgtttcgta atatatccaa tctttctatt ttctgcaagc
    32701 ataaaaaagc cgccatgtgg cggatagggt cgcggtcttc aacagcatgt aggatttagg
    32761 aatgcccaaa ttgggcgcag gggtgttgaa aacacgcaaa cagacgcgcc gccagcctta
    32821 cgggtaggcg cacccccaca taggagtaaa tcgatttgat acgtatagac gtaaaaaatt
    32881 ccactctatc gtgttggata tacgctgttt gtcaggtgtt ttcaagcacc gtgggaaata
    32941 ttatagagtt atggaaatgt gtcaagagaa ttagcccaaa tgggcggacg cgcggttaag
    33001 aacccacata ggagggcgga cttattcccc tttcgggtct tgtattcgtc gcccacgcgg
    33061 tcatagaaac ttcctgctat cgaaacaaac aacaaggaaa gaaaactata gacatgaaaa
    33121 aatcacattg acggagtgat tgccgctgta gtgtggtttc tgacgccacg aacaggaata
    33181 taaaacaaaa cccctgcaca tgcaaggggt ttcccaaaaa accgtaggcg gcaaactgaa
    33241 aggccgtctg aatttcagac ggcctatgtt gcggcggatg ttaagctatt aagctatttg
    33301 gttttcagag gctttttctt ccattttcgg gctacggctt caaattcttg aaaggccgtc
    33361 ggtgcgctcc tgatacggcg tatttcaaaa atgaagctgc ttaggttgtc ccagtcacgt
    33421 aacagcatgg agtattcgaa atctttatac attttctcgt gcagtgctcc gcccaatacg
    33481 cccgcacaga caaattcgcg ctggtttaaa actgtcagta tggcttcacg gtctttcttc
    33541 ttgtccgggg tatccgatgt ataggtggcg agtatgcatc cgtctgtttt tgccagcccg
    33601 tttactatgg ttatggcttc ttgaagggcg gcattgttac gttcggccat gatcatgtcg
    33661 atggtggcgc gctttttggc gttttctgta ttcctcatga tgccgtaagc ggcaacaaaa
    33721 acgccgataa cagtcaatat cggcgttgct atctgtatta ggttgtcagt cattgccgtc
    33781 ccagccgtct gaaaggcgga aatcacggct ttccgaataa atatgtccca ttgtcatccc
    33841 cttaagctga tgttgaaaat aaatccttgc gtcggaatat acggcattgc ttgactattt
    33901 tcaagattct gtacctcgtg aaccggacta tacaaaaaaa ccgccttcgt gtaaagcggt
    33961 tcttaaatcg cccgttaagg cggacacaac gatagccaaa ctggaacagg aggcggcaaa
    34021 ggcgggtttg tccaaaacag ccttgattga aaggctgatt ggggatttgt aggcgtttgc
    34081 agatacgaca aggggcgcat tccgcgcccc atcacacgca cggacggctt gtcatcgcct
    34141 gtcgcccgtg cgcggctgcc catatttcag aacagtcttg caagcccctt taagggaacg
    34201 gttttattct agtacagttt gaatgccttg gcaacggctg ttttaatcgc ctgaaaatcc
    34261 tcttcactga ttattggaat gcagcggtcg cgccctttgg gtttgtatcg gtctaatcgt
    34321 gccaatccga ctgttgccgt catgtcgcat tttgcccaac attggatgtg cggcttgtcc
    34381 ggtaaggggt ttccactcat tttgtggtgg tagtccgcca aagggacagg ttctgtgctg
    34441 cttaagggta cgaccgttac cagtttgccg ttgtgcctgt ttcgcgctat gacgacgaca
    34501 gggcgtttct tgaccatttc cggttcttca taaccgcgaa agtcgcacat gataaccgaa
    34561 cgttcccttg gttggaattt taaaggcatt agccgctcct gacgatgaca ggctgctatt
    34621 atagcagttc ttacacaaaa aaccgccttc gtgtaaagcg gttgcaaaaa agccttccaa
    34681 taaaaatgcg gaaacggttt tttatttgcg tttccgcata atacaaacac acacacaaaa
    34741 aaatcatttt ccgtttttgg caagcctcaa aacatcattc ccccaccgtt ccagcgcatc
    34801 cttacgctcc tcccaaagct ccgagcggtt gtaagccgtt tgcgtcctcg tccccaacga
    34861 atgggcaagc agcttttccg ccacatcatt atccaccttc agaacctcgc gcaaataagt
    34921 acgcgcaagc aagcgcaaac cgtgcgccgt cgtatccagc cccgcccgct gcatcgccac
    34981 gcgcggactt tccttattga tatgcccttg aaaattcaca ctttcaaaca caaaccgccc
    35041 gttcacattc aacgcccgcg cctgatccaa cacccactgc atcgcccgcg acagcggcac
    35101 atcatgcccc cgccgctcct ttttccgttc ggcggggata cgccaaacct ccccgtccaa
    35161 ctccgaccac tccatcaacg ccgcctcctg aatccgcgtc attgtcaaca gcagccaata
    35221 gatacaaagc cgcgttacag gatggatacg caccctgccc gcataaaccg caaattcatt
    35281 gcgctgctcc aaaaaatcaa tcaggcgcgg caattcagac ggactcaacg ccgccatatt
    35341 ccccgttttc gcccgttcaa acacacccga accgatttgc gcgacagggt tgatttccat
    35401 cattcccgaa ccgaccgcaa acgcaaacac catcttcaga ctgtttttcg ttttgcgcaa
    35461 cgtatcaacg ataccgcgcg cctccatcac acgcagacag ccgaccacat ccgccgtcct
    35521 gacttgacga atatcaagat tgccgatagc cggaaaaacc caccgctcaa aattccgcat
    35581 aacctgtccg gcatactttt cagaccgccc cttcgaccaa cgcacaaacc aatcacgcgc
    35641 caccttctca aaagcaaaat ccgcccgcac cttcttattg acgacatttt ccccgtgcgc
    35701 ccgttttcgg cgcacctcct cccgccattc ccgcgcatcg gccagcgaaa aatcaggata
    35761 ccgccccagc gaaattgtct gctgccttcc atcctgcaca aacgacagct tccaactccg
    35821 cccgcccgtc gggtagaccc acaaagccag cccgcccccg tccgacaact tatacagctt
    35881 atcgcgcggc ttcgcatttt taacctgatt tgccgacagc ggcgtaatga ttttcgccat
    35941 tatggtaatt tcccatttat ccaaaaatta ccatacacat taccataaaa aaaatgcaat
    36001 aagaagatgc cgcaagattg caaatgattt actttgacgc gttttgatag ggcaaaccga
    36061 tgatttttat atggtaattg acacaacttg aacaaaaaag aaccgccctg aatcagggcg
    36121 gttttgtttt ctggcggaaa cggtgggatt cgaacccacg gaggatttgc accctcagcg
    36181 gatttcgagt ccgctgcatt cagcctctct gccacgtttc cgataatgaa gtaaaaccaa
    36241 ataaaaatgc cgatgcttta tgctacttca tttttatttg gtttttgacc tcaaggattg
    36301 gcggaagcgg tgagattcga actcacggag ggctatcaac cctcgacggt tttcaagacc
    36361 gttgcattaa accgctctgc cacgcttcca tcttcttgaa gaagacgaat aataatgaag
    36421 tttatacatt ttgccaagca cttttttcgg aaaatactta atttattgtt ttcactttat
    36481 ttgttccgaa cgcataaaca cccattcact ttcattggaa gccgcgtcat tgaatgcgta
    36541 gccttcgtaa ttaaaatttt tcagcatttc aggatcggta atattgtgtt ctgccgcata
    36601 gcgcaccatt aatccgcgcg cgcgcttggc atagaaactg atgattttat atttaccgtt
    36661 tttttcgtct ttaaatatcg gggtaatcag ccgcgcccga agtgttttcg tgttgacgga
    36721 cttgaaatat tcctgtgagg caaggttgac aagcgtattg ctgcctgctt gggcaagcgt
    36781 gtcatttaaa aggttggtaa tgatgtctcc ccaaaactca tacaaattct tcccgcgcaa
    36841 attggcaaat gccgtcccca tttccaaacg gtaaggctgt atcaggtcca gtggacgcag
    36901 aaggccgtac aggccggaca gcaggcgtac gcgcccttgc agatattgta tttgatcggt
    36961 attcaatgtg tttgcatcca taccttcgta aacatcgccg ttgaacataa agaccgcctg
    37021 tttggcgttt tccggcgtaa acggcgtgtt ccattctgca ttgcgctgcg cgtttaagag
    37081 ggcaattttg tcggaaacgt gcatcagttc ggcaatctgt tgcggcgcaa gctcgcgcaa
    37141 ctgctgcatt agaatgtcgg actctgccag caggtcgggt tgggtaaact cgctgacagg
    37201 ggcagggtct ttttcattaa ggttcttcgc aggggaaagg acaaaaaaca taatctgctc
    37261 ggtattgtgt caaacacggg gattttaagt ttaaacgtgt ttgcgggcaa gccgattaaa
    37321 agtgaagtat agtggattaa atttaaacca gtacagcgtt gcctcgcctt gccgtactat
    37381 ctgtactgtc ttcggcttcg tcgccttgtc ctgatttttg ttaatccact ataaataccg
    37441 ttacagcaaa ttcaaccgta gaatattgaa aaatgccgtc tgaacaaaag ttcagacggc
    37501 atttccgatt ccttaccgat acagtgaaac tatgtcttcc gcagctttga cacaagttgc
    37561 gacatcggca accaaagcga tacggacata accttccccg ggattgccct gttcggtatc
    37621 ccgtgccaaa aagcgtccgg gtaatacttg gatagcggct ttttgccata aattgcgtgc
    37681 aaatgccaaa tcgtcgccat cggggacttt caaccagatg taaaacgagg catccggtaa
    37741 tttgacgtca aatacctgtt gcaggatagg aataacccat tcaaactttt cctgatacag
    37801 gcggcggttg tcaataacgt gctgttcgtc attccaggcg gcaatactgg cacgctgtac
    37861 gggaatgctc attgcgctgc cgtgataggt tctgtaaagc agaaaatttt taagcagttc
    37921 ggcatcgccg gcgacaaaac cggaacgcag gcctggaaca ttggaccgct tggacaaact
    37981 ggtgaacata agcagttttt gcctgcttcg ccccaactgt gcagcggcct gcaggcagcc
    38041 caaaggcttg ttgccgtcga aatagatttc ggaatagcat tcgtccgagg caataatgaa
    38101 accatatttg tcctgcaaat caaaaacttc tttccatccg tccaaatcca gcacgctgcc
    38161 gctggggttg ttgggcgagc agacgaacac cagtttggtg cgtttccaaa cctcttcgga
    38221 aatactacgc caatcggggt tgaaagacgg cgcggggcaa ttggcaaaat ggatttcacc
    38281 gccgcccaaa agtgtcgcac cttcataaat ctgataaaag ggattcgggc tgacgattac
    38341 gggtttgagg ccgtctgaaa cagggttcaa cacggtttgg acaaaagaaa acaacgcctc
    38401 cctacttccc aaaaccggca gaatttcggt gtcggaattc agcgtcaacc catcataacg
    38461 gcgttttaac cagttcgcac acgcctgacg cagttcaggc aaaccggccg tcagcggata
    38521 tttttccaac tcgtgcaatg aggcggtcag cgcatccgta atgactttcg gtgtcggatg
    38581 tttcggttcg ccgatatgca gtgggacggc ttccagacct tcgggggcgg aaatgccctg
    38641 catcgcttca cgcagtcggg caaagggata gggtttgagt tggttgagta aggtattcat
    38701 gattttctcc ggttgtcggt atatcggaag atatgccgtc tgaacctgac gtgcccttca
    38761 gacggcattt tatattcaat gcaaacccgt actgccgaag ccgccctcac cccggctgct
    38821 tccgacaaat tcctcgacac gtttgaaacg cgcctgcacg attggcacga caaccatctg
    38881 cgcgatacgc tcaaacggtt tgacagtaaa cggttcgctg cttctgttcc ataacgacac
    38941 cttcaattcc ccttgataat cggagtcaat caaaccgacc aaattgccca aaacaatgcc
    39001 gtgtttatgc cccaagccgg aacggggcag caaaacggcg gcatatgcgg gattcgccaa
    39061 ataaattgcc aaacccgtcg gcacaagaaa cgtttcaccc ggctgcaaaa cgacttcctc
    39121 atccaaacag gcgcgcaaat ctaaacctgc agaacccttc gttgcataga cagggacaac
    39181 atccgccatc cgttcgtcca atactttcat ttctacttca atattcatcg tcttgctcct
    39241 gcatacggcc ggtttcaaag atgggagatt atacacaagc cgtgtttacg ctgaaacaca
    39301 gcaaataggt tttgttttga aatactccgt ctgtgtataa tgcccgacct tcttacatat
    39361 caatattaat atgacacaca taagctcgcc ccgtaacgaa tttatacgcg gcatcaaaga
    39421 aagttcgccc atgctgatcg ggcttttgcc ttgggcatta atactcggta tgcagggtgg
    39481 acaaaaaggc atgagctggc tggaaatgtt gttgatgacc ggtatgaact tcgccggcgg
    39541 ctccgagttt gccacggtca acctgtgggc ggaacctctg ccgatactgc ttatcgccac
    39601 cgtaaccttt atgattaatt ctcggcatat cctgatgggg gcggcacttg ccccgcacct
    39661 gaaagaaata ccgctgaaaa aagccgtgcc cgcactgttt tttatgtgtg atgaaagctg
    39721 ggcgatggca ttctccgaaa tccaaaaacg gaaagcagcc ggtttgcccg cattcaatat
    39781 gcct
//
LOCUS       DAWKST010000002        30070 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.002, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000002 DAWKST010000000
VERSION     DAWKST010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30070
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.002"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            91..723
                     /locus_tag="WJL87_000052"
     CDS             91..723
                     /locus_tag="WJL87_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HEZ7798550.1"
                     /translation="MDETNTKADEAVKTANEAKQTAEETKQNVDAKVKAAETAAGTAN
                     TAADKAEAVAAKVTDIKADIATNKDNIAKKANSADVYTREESDSKFVRIDGLNATTEK
                     LDTRLASAEKSITEHGTRLNGLDRTVSDLRKETRQGLAEQAALSGLFQPYNVGRFNVT
                     AAVGGYKSESAVAIGTGFRFTENFAAKAGVAVGTSSGSSAAYHVGVNYEW"
     gene            complement(789..1127)
                     /locus_tag="WJL87_000053"
     CDS             complement(789..1127)
                     /locus_tag="WJL87_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ7798551.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERSDAAV"
     gene            1278..5240
                     /gene="purL"
                     /locus_tag="WJL87_000054"
     CDS             1278..5240
                     /gene="purL"
                     /locus_tag="WJL87_000054"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ7798552.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPGLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFELREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPIDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHADDIDITEAAYRVLHLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVQDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTGRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLDIDLNLLLA
                     QTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVADIINLFYQ
                     QQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRLRDNPACAD
                     SEFALIGDNDRSALFANLKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFT
                     RAGFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQ
                     FAAFFADPDTLTLGVCNGCQMVSNLAEIIPGAEAWPKFKRNLSEQFEARLSMVHVPKS
                     ASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNP
                     NGSPQGIAGVTNADGRVTIMMPHPERVYRAAQMSWKPEDWTELSGWYRLFAGARKALG
                     "
     gene            5335..6093
                     /gene="gloB"
                     /locus_tag="WJL87_000055"
     CDS             5335..6093
                     /gene="gloB"
                     /locus_tag="WJL87_000055"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ7798553.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLDFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            6407..10882
                     /gene="mspA"
                     /locus_tag="WJL87_000056"
                     /gene_synonym="ausI"
     CDS             6407..10882
                     /gene="mspA"
                     /locus_tag="WJL87_000056"
                     /gene_synonym="ausI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/serine protease MspA"
                     /protein_id="HEZ7798554.1"
                     /translation="MRFTHTTPFCSVLSTLGLFAVSPAYSSIIRNDVDYQYFRDFAEN
                     KGAFAVGASNISIQDKQGKILGRVLNGIPMPDFRVSNRQTAIATLIHPQYVNSVKHNV
                     GYGSIQFGNDTQNPEEQAYTYRLVSRNPHPDYDYHLPRLNKLVTEISPAALSSVPLLG
                     NGQPKANAYLDTDRFPYFVRLGSGTQQVRKADGTRTRTAPAYQYLTGGTPLKVLGFQN
                     HGLLVGGSLTDQPLNTYAIAGDSGSPLFAFDKHENRWVLAGVLSTYAGFDNFFNKYIV
                     TQPEFIRSTIRQYETRLDVGLTTNELIWRDNGNGDSTLQGLNERITLPIANPSLAPQN
                     DSRHMPSEDAGKTLILSSRFDNKTLMLADNINQGAGALQFDSNFTVVGKNHTWQGAGV
                     IVADGKRVFWQVSNPKGDRLSKLGAGTLIANGQGINQGDISIGEGTVVLAQKAASDGS
                     KQAFNQVGITSGRGTAVLADSQQIKSENLYFGFRGGRLDLNGNNLAFTHIRHADGGAQ
                     IVNHNPDQAATLTLTGNPVLSPEHVEWVQWGNRPQGNAAVYEYINPHRNRRTDYFILK
                     PGGNPREFFPLNMENSTSWQFIGNNRQQAAKQVAQAENARPDLITFGGYLGENAQTGK
                     AAPSYSKTNIEWVNGPNTPHKPSPYTNTPTRTGAGVPTITASKKAATPAPTSHGMPTA
                     PKTGNFSDMTGEAAIEKNRHIANAAVYGRPEYRYNGALNLHYRPKRTDSTLLLNGGMN
                     LNGEVLIEGGNMIVSGRPVPHAYDHQAKREPVLENEWTDGSFKAARFTLRNHARLTAG
                     RNTAHLDGDITAYNLSGIDLGFIQGKTPECYRSYHSGSTHCTPNAVLKAENYRALPVT
                     QVRGDITLNDRSELRLGKAHLYGSIRAGKDTAVRMEADSNWTLSQSSHTGALTLDGAQ
                     ITLNPDFANNTHNNRFNTLTVNGTLDGFGTFRFLTGIVRKQNAPPLKLEGDSRGAFQI
                     HVKNTGQEPQTTESLALVSLNPKHSHQARFTLQNGYADLGAYRYILRKNNNGYSLYNP
                     LKEAELQIEATRAEHERNQQAYNQLQATDISRQVQHDSDATRQALQAWQNSQTELARI
                     DSQVQYLSAQLKQTDPLTGILTRAQNLCAAQGYSVDICRQVAKAADTNDLTLFETELD
                     TYIERVEMAESELDKARQGGDAQAVETARHAYLNALNRLSRQIHSLKTGVAGIRMPNL
                     AELISRSANTAVSEQAAYNTGRQQAGRRIDRHLTDPQQQNIWLETGTQQTDYHSGTHR
                     PYQQTTNYAHIGIQTGITDRLSVGTILTDERTNNRFDEGVSARNRSNGAHLFVKGENG
                     ALFAAADLGYSNSRTRFTDYDGAAVRRHAWDAGINTGIKIDTGINLRPYAGIRINRSN
                     GNRYVLDGAEINSPAQIQTTWHAGIRLDKTVELGQAKLTPAFSSDYYHTRQNSGSALS
                     VNDRTLLQQAAHGTLHTLQIDAGYKGWNAKLHAAYGKDSNTARHKQAGIKIGYNW"
     gene            11114..12568
                     /gene="mgtE"
                     /locus_tag="WJL87_000057"
     CDS             11114..12568
                     /gene="mgtE"
                     /locus_tag="WJL87_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ7798555.1"
                     /translation="MSIEHTPPTHDGETGQNHAERPSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLSELHPADVAAVLESLPPRERNIVWILVKSEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRVTVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTGMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(12654..13562)
                     /gene="hslO"
                     /locus_tag="WJL87_000058"
     CDS             complement(12654..13562)
                     /gene="hslO"
                     /locus_tag="WJL87_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEZ7798556.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENEWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGQGRLKMLVAEATSDRTVRATARWDEA
                     AEIGEDESLVDLLGEGGVFVLTLQPKDGEPWQGVVPLEGDGIAQMLMNYMKRSEQLDT
                     HIVLSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAGELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVVEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHIVQ"
     gene            13807..14562
                     /locus_tag="WJL87_000059"
     CDS             13807..14562
                     /locus_tag="WJL87_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ7798557.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSISTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            14581..14799
                     /locus_tag="WJL87_000060"
     CDS             14581..14799
                     /locus_tag="WJL87_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798558.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            14944..15288
                     /locus_tag="WJL87_000061"
     CDS             14944..15288
                     /locus_tag="WJL87_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ7798559.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPDSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            15288..15980
                     /locus_tag="WJL87_000062"
     CDS             15288..15980
                     /locus_tag="WJL87_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ7798560.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVIVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            16047..17267
                     /gene="argJ"
                     /locus_tag="WJL87_000063"
     CDS             16047..17267
                     /gene="argJ"
                     /locus_tag="WJL87_000063"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ7798561.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALIINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVVD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQSAATVYTCD
                     LSHGYVSINADYRS"
     gene            17327..18736
                     /locus_tag="WJL87_000064"
     CDS             17327..18736
                     /locus_tag="WJL87_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ7798562.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGVSIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGVPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETAAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            18925..20316
                     /gene="hrpA"
                     /locus_tag="WJL87_000065"
     CDS             18925..20316
                     /gene="hrpA"
                     /locus_tag="WJL87_000065"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ7798563.1"
                     /translation="MFPDFSQTLSKDRHFLQSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLDEIKKTVAEHQVTIICGETGSGKTTQLPKICLELGRG
                     VAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARHGEGDI
                     LVFLPGEREIRETAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHVEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMASLNLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAV"
     gene            20417..21544
                     /locus_tag="WJL87_000066"
     CDS             20417..21544
                     /locus_tag="WJL87_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798564.1"
                     /translation="MKHLLIDFENVQPQNLDKLPTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKITTEPPSALLENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQRPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQDTQVLRIDGTKIVYIK"
     gene            21722..23298
                     /gene="lpt3"
                     /locus_tag="WJL87_000067"
                     /pseudo
     CDS             21722..23298
                     /gene="lpt3"
                     /locus_tag="WJL87_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
     gene            23400..26501
                     /locus_tag="WJL87_000068"
     CDS             23400..26501
                     /locus_tag="WJL87_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEZ7798565.1"
                     /translation="MDARSNPANVSDDLQNSSGHIGVNTRYRLTKLGEQMARLPIDPK
                     IARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHERFTDKQSDFLAYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRPPEGRQLTSSENAGDQDLSAKLKQKQLDKKQHRAQIRAAKEAGYEQIHRALLTGL
                     IANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVETTRLYARDVAVIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRPVSYGKVAPEEAR
                     EIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYNERLP
                     DFYTADAVSDDLHPTNPQQTAPSYAREERREGKTVAAQTNFSATAANPLPNPLPQERE
                     QSAAASAVSNDLHPANPQQTAPSPVGEGRGEGKTVASQTNFSATTANPLPNPLPNPLP
                     QEGEQSAAASAVSNDPQPQKQPAPQKDRLKPLPLADIRTFQVWLKTAERENPRLLFLS
                     RDDLMQHAAAHITEEQFPKFWQTADGKFKLSYRFEPHHPLDGVTLTLPLTVLNRLHAP
                     SLEWLVPGMLREKIQLLIKALPKQIRRICVPVPDFITQFLSQNPDRNAPILPQLAQAI
                     AKTAGDIRILEQINQDEWAAFRLPEHCYFNLRIIDDGGQELAGGRKLHELQQQLGQAA
                     AVTFRDNTQEFERDNVTTWDIGTLPESIKFARGKQQLTGYLGLQKEKDGRIALRLCDT
                     TEAAEQAHRQGVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLCDDLTQAVC
                     DRAFIGEDELPRNEKAFKEQIKRARSRLPAVKEALSRYLQETAAAYAELNSKLGKHPL
                     THLLRQRLQTLLAAGFATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQE
                     LEQMWQEKTDSLIKQGLPISDGLAAFKWMIEELRVSLFAQELKTPYPVSVKRLLKMWE
                     DLN"
     gene            complement(26524..26946)
                     /locus_tag="WJL87_000069"
     CDS             complement(26524..26946)
                     /locus_tag="WJL87_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HEZ7798566.1"
                     /translation="MTDIFTTSKRSFVMSKIHSKETKPEILVRKFLFSQGFRYRKNDK
                     RYAGKPDIVLPKYKTVVFIHGCFWYGHSCNKGHIPKSNTDFWLEKITKNCERDIKNET
                     ELEKIGFKVIVVWECELKNKAIGRERLNRLVREIKDAV"
     gene            complement(26950..28596)
                     /locus_tag="WJL87_000070"
     CDS             complement(26950..28596)
                     /locus_tag="WJL87_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HEZ7798567.1"
                     /translation="MSEEKLKMSFEPTVIEHLGVKMYSHTVPAIAELIANAYDACATE
                     VEVRLFDKPEHKIVIKDNGIGMSFDEINDFYLRIGRNRREEKQASPCGRIPTGKKGLG
                     KLALFGLGNKIEISTIQGNERVTFTLDYAEIKKSERIYQPEFQKESVEPNTENGTTIT
                     LTELTKKQGYPLDNYVEHLSRLFDFPAQDFKIKVSLNGSEPKIIDGNLKYDLVTPQFE
                     WEYQDLATNISSLSSKFEQYEYSGLIQGKFITTEKPLKNNMKGITLFANGRMVNMPEF
                     FTDSESSHFYSYLTGWLSIDFIDNDDEDLIATDRTSLDWKHPKTSKLRVFLGEIVRAI
                     GQDWRKRRKKEKDDEVKGESGVDIENWKNKLPEKEREPVEVILNRLEDSELTNKEQAE
                     VISALHSIIPEYPYYHWRHLHQDLHTACNDFYNEKKDYLSAAIEAVKVFENKVQKQTG
                     LHSIDGRELIEQAFGSKNSILLLTNNKTKAEQNLEDGLEQLACGTWTGFRNPVQHELR
                     ANLSPSIFNDKDALDLISLVSYLLRKVEQTKKRSKVVSSK"
     gene            complement(28610..29665)
                     /locus_tag="WJL87_000071"
     CDS             complement(28610..29665)
                     /locus_tag="WJL87_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ7798568.1"
                     /translation="MKNSKLKAVDFFCGGGGMSYGMQSAGIQVLAGIDYEPSCKETYE
                     ANINAKFIQADVFELQPETLEKELGLKKNDDDLILIGCSPCQYWSVIQTDKRKSEKSK
                     SLLLEFQRFVEYFNPGYVVVENVPGILSRMKESRLDNFIKLLEEKGFTVHFGIHNTAD
                     YGIPQSRKRFTLIANRITKEKLEPVKYSGKRLTVRDVLGMENGFPKIIAGHQDETDFM
                     HSCAGLSDINLKRLALIPKNGGNRLAFAHIPELQLECFIGKDNSFKDTFGRLWWDKPA
                     PTITTKFFSISNGRFAHPEEDRALSLREGATLQSFPRNYVFKAGSRDKIARLIGNAVP
                     PMYAEKIGRAIVDNIEC"
BASE COUNT         7624 a         9021 c         7038 g         6387 t
ORIGIN      
        1 acaaatatcg taaaaattga tgaaaaatta gaagccgtgg ctgataccgt cgacaagcat
       61 gccgaagcat tcaacgatat cgccgattca ttggatgaaa ccaacactaa ggcagacgaa
      121 gccgtcaaaa ccgccaatga agccaaacag acggccgaag aaaccaaaca aaacgtcgat
      181 gccaaagtaa aagctgcaga aactgccgct ggcacagcta atactgcagc cgacaaggcc
      241 gaagctgtcg ctgcaaaagt taccgacatc aaagctgata tcgctacgaa caaagataat
      301 attgctaaaa aagcaaacag tgccgacgtg tacaccagag aagagtctga cagcaaattt
      361 gtcagaattg atggtctgaa cgctactacc gaaaaattgg acacacgctt ggcttctgcc
      421 gaaaaatcca ttaccgaaca cggtacgcgc ctgaacggtt tggatagaac agtgtcagac
      481 ctgcgtaaag aaacccgcca aggccttgca gaacaagccg cgctctccgg tctgttccaa
      541 ccttacaacg tgggtcggtt caatgtaacg gctgcagtcg gcggctacaa atccgaatcg
      601 gcagtcgcca tcggtaccgg cttccgcttt accgaaaact ttgccgccaa agcaggcgtg
      661 gcagtcggca cttcgtccgg ttcttccgca gcctaccatg tcggcgtcaa ttacgagtgg
      721 taagcagcat ctcccgataa agaaaccgca gccctgcaag gctgcggttt ttattttcta
      781 tccggccgtc aaaccgccgc gtccgaacgt tcgcccgtgc ggatacggat tgcctcctcg
      841 accggcagca caaaaatctt gccgtcgccg atttttcccg aacgcgccac ctcgacaatc
      901 acgtcaatcg cgcgttccac agcatcatcc gccaacacca gctcgatttt gaccttgggc
      961 aggaaatcga cagcgtattc cgcgccccgg taaatctccg tatgcccctt ctgcctgccg
     1021 aaccctttga cctcgctgac ggtcataccc gtaatgccga tttccgtcaa cgcctcgcgc
     1081 acgtcgtcga gtttgaacgg tttgacaatc gcctcgattt ttttcataaa atttcctttg
     1141 aacaaacaat acaaacacat ccgaaaaacg ggaacctccc gtcagattgt caacatttta
     1201 agccaaaata cccaagcaat acagccccgt tgcgcgtata atgacagatt ttccaaccgc
     1261 atttgagagc cgaatccatg tctgtcgttt tgcccttgcg cggcgttacc gccctttccg
     1321 atttccgtgt cgaaaaactc ttgcaaaaag ccgccgcact cggactgccc gaagtcaaat
     1381 taagcagcga attttggtat ttcgccggca gcgagaaagc attggatgcc gcgactgtcg
     1441 aaaaactgca agccttgttg gcggcgcaaa gcgttgaaca aacgccaaaa gcgcgcgagg
     1501 gcttgcattt gtttttggta acgccccgtt tgggtacgat ttcgccgtgg gcttccaagg
     1561 cgaccaatat cgcggaaaac tgcggtttgg caggcatcga gcgcatcgag cgcggcatgg
     1621 cggtgtggct ggaaggtgcg cttaccgatg aacagaaaca gcaatgggcg gcgttgctgc
     1681 acgaccgcat gaccgaatcc gtgctgcccg attttcagac ggcatccaaa ttattccacc
     1741 atctcgaatc cgaaaccttt tccaccgttg atgttttagg cggcggtaaa gaggctttgg
     1801 tcaaagccaa taccgaaatg ggtttggcac tctccgccga cgaaatcgat tatctggttg
     1861 aaaactatca ggctttgcaa cgcaatccgt ccgatgtgga gctgatgatg ttcgcgcagg
     1921 caaacagcga acactgccgc cacaaaatct tcaacgccga cttcatcctc aacggcgaaa
     1981 aacaaccgaa atcccttttc ggcatgatac gcgacacgca caacgcgcat cccgaaggca
     2041 cggtcgtcgc ttataaagac aattcgtccg tgatcgaagg cgcgaaaatc gagcgtttct
     2101 atccgaatgc ggcggaaaac caaggctacc gtttccacga ggaagacacg catatcatca
     2161 tgaaagtgga aacgcacaac cacccgaccg ccatcgcgcc gtttgcaggc gcggcaacgg
     2221 gcgcgggcgg cgaaatccgc gacgaaggcg caacgggcaa aggttcgcgt ccgaaagcag
     2281 gcttgaccgg ctttaccgtg tccaacctca acatccccgg cctcaaacag ccgtgggaac
     2341 aagattacgg caagccggaa catatttcct cgccgctgga catcatgatt gaaggcccga
     2401 tcggcggcgc ggcgttcaac aacgaattcg gccgccccaa cctcttgggc tacttccgca
     2461 cttttgaaga aaaatttgac ggtcaggttc gcggctacca caaaccgatt atgattgccg
     2521 gcggcttggg cagcattcag gcgcagcaga cgcataaaga cgaaatcccc gaaggcgcgt
     2581 tgctgatcca actgggcggc ccgggcatgc ttatcggctt gggcggcggc gcggcttctt
     2641 cgatggatac cggcacgaac gacgcttctt tagacttcaa ctccgtacaa cgcggcaacc
     2701 ccgaaatcga acgccgcgcg caggaagtca tcgaccgctg ctggcagctc ggcgacaaaa
     2761 acccgattat ctccatccac gacgtgggcg caggaggttt gtccaacgcc ttccccgaac
     2821 tggtcaacga tgccggacgc ggcgcggtat tcgagctgcg cgaagtgccg ttggaagaac
     2881 acggtttaac gcctttgcaa atctggtgca acgaatcgca agagcgttat gtgttgtcga
     2941 ttttggaaaa agatttggat accttccgcg ccatctgcga acgcgaacgc tgcccgtttg
     3001 ccgtggtcgg tacagcgacc gacgacggtc atttgaaagt gcgcgacgat ttgttcgcca
     3061 acaaccccat cgatttgccg ttgaacgtct tgctcggcaa accgcccaaa accacgcgta
     3121 ccgacaaaac agttacgcca tctaaaaaac cgtttcacgc cgacgatatc gacatcaccg
     3181 aagccgccta ccgcgttttg cacctgcctg ccgtagccgc caaaaacttc ctgattacca
     3241 tcggcgaccg cagcgtcggc ggcatgaccc accgcgacca aatggtcggc aaataccaaa
     3301 cccccgtagc cgactgcgcc gttaccatga tgggcttcaa tacctatcgc ggcgaagcaa
     3361 tgtctatggg cgaaaaaccg accgtcgccc tgtttgacgc gcctgcttcg ggcagaatgt
     3421 gcgtcggcga agccatcacc aacatcgcgg cggtcaacat cggcgacatc ggcaacatca
     3481 agctctccgc caactggatg gcggcgtgcg gcaacgaagg cgaagacgaa aaactctacc
     3541 gcaccgtcga agcggtttcc aaagcctgtc aggcattgga tttgagcatt cccgtgggca
     3601 aagacagcct gtcgatgaaa accgtatggc aggatggcga agagaaaaaa tccgtcgttt
     3661 cgccgttgag cctgattatc tccgcgttcg cgccggttca agacgtacgc aaaaccgtta
     3721 cgcccgaatt gaaaaacgtc gaggacagcg tattgctgtt tgtcgatttg ggcttcggca
     3781 aagcgcgtat gggcggctct gcattcggtc aggtatacaa caacatgagc ggcgacgcgc
     3841 ccgatttgga cgacacaggc cgtctgaaag cgttttacaa cgtgattcaa cagcttgtcg
     3901 ccgaagacaa actcttggca taccacgacc gcagcgacgg cggcttgttt gccgttttgg
     3961 tagaaatggc gtttgcaggg cggtgcggct tggatataga tttaaattta ttgcttgcac
     4021 aaacatttat taccaaccat accgctctgt ctcaatcatt gcggactgaa gaggtaaaag
     4081 cgttggctga atggcaagaa accattgccc gcacattatt taatgaagag ttgggtgctg
     4141 ttatccaagt tagaaaacaa gatgttgccg atattatcaa tttattctat caacaacagc
     4201 tgcatcataa tgtctttgaa atcggtacgt taactgatga gaacacgtta atcatccgcg
     4261 acgggcaaac gcaccttatt tctgacaacc taatcaaact gcaacaaacc tggcaagaaa
     4321 ccagccatca aatccaacgc ctgcgcgaca accctgcctg cgccgacagc gagtttgccc
     4381 tgattggcga caacgaccgc agcgcattgt ttgccaacct gaaattcgac gtgaacgaag
     4441 acatcgccgc gccgtttatc aacagcggcg cgaaacccaa aatcgccatc ctgcgcgaac
     4501 agggcgtgaa cgggcaaatc gaaatggccg ccgccttcac ccgcgccgga ttcgatgctt
     4561 acgacgtgca tatgtccgac ctgatggcag gccgcttccg ccttgccgac ttcaaaatgc
     4621 tggcggcgtg cggcggcttc agctacggcg acgtactcgg cgcgggcgaa ggctgggcga
     4681 aatccatcct gttccaccct gccttgcgcg accaatttgc cgccttcttt gccgatccgg
     4741 acacgctgac attgggcgtg tgcaacggct gccagatggt cagcaacctc gccgagatta
     4801 tcccgggtgc agaggcatgg cctaaattca aacgcaacct gagcgaacag tttgaagcac
     4861 gcctgagcat ggttcacgtt ccgaaatcag cgtcgctgat tctgaacgaa atgcaaggct
     4921 ccagcctgcc tgtcgtggtc agccacggcg aaggccgcgc cgacttcgcg cttcacggcg
     4981 gcaatatttc cgccgatttg ggcatcgcgc tgcaatatgt ggacggtcaa aaccaagtga
     5041 cccaaaccta cccgctcaac cccaacggct cgccgcaagg catcgccggc gttaccaacg
     5101 ccgacggccg cgtgaccatc atgatgccgc atccggaacg cgtgtaccgt gccgcacaaa
     5161 tgagctggaa accagaagac tggacggaat tgtccggctg gtaccgcctc tttgccggcg
     5221 cacgtaaagc cttgggctaa ccgccctact caaaccaatg ccgtctgaag aatatttcag
     5281 acggcgttcc ggcataccat cctttaaacg gtatccgtcc accgaggaac actcatgaaa
     5341 atcacccccg tcaaagccct aaccgacaac tacatctgga tgatacagca cggcaaccat
     5401 gccgtctgcg tcgacccttc cgaaccctcg cccgtcttgg aattcctcgt ccgcaaccgc
     5461 ctcatgcttg cccaaacatg ggtaactcac ccccaccccg accacgaggg cggtgcggcg
     5521 gcactctggc gcggctacat ggaatcgccc gtttacggcg aatccgacat cgaagcagca
     5581 acccacaccg taaccgccgg cacccaattc accttcggcg acggacaggt taccgtttgg
     5641 gcaacacccg gccacacaga ccgccacacc agctaccttc tcgaaacttc agacggcata
     5701 cacgttttct gcggcgacac cctgttttcc gccggctgcg gacgcgtgtt taccggcaca
     5761 atcgaacagc tttacgacag cttccaacgc ttcaaccgcc tgcctgaaaa caccctgttc
     5821 tacccggcac acgaatacac cgccgccaac ctggattttg ccgcccatat cgagccggac
     5881 aacgccgaca ttcagacggc actgaaagcg gcggcgcata cgcctaccct gcccgttacc
     5941 ctcgcgcacg aacgccgcgt caatccgttt ttgcgcgtcg acctgccgca cgtcagagac
     6001 cgcgccgagg cattgagcgg gaaaacgtta aacagcagcc tcgatacctt tgtcgcgctg
     6061 cgtgaactta aaaaccaata ccggacgaaa taaaacaacg ggaaaacgca gccattccta
     6121 ggatttttat taaaatctta aataaaatca tacaatcatc gccaatagac gaaaggacac
     6181 cgttgcctta taatcaaaca aaaacaaaat atataatata gtggattgaa tttaaatcag
     6241 gacaaggcga cgaagccgca gatagtacgg caaggcgagg caacgctgta ctggtttttg
     6301 ttaatccact atattgttaa tccactatat aaatccagca caaaacggga tcggtgattc
     6361 ttgtccgcaa gaatcgttga ttttctctat tacacggata atcatcatgc gcttcacaca
     6421 caccacccca ttttgttccg tattgtccac cctcggtctt tttgccgttt cccctgctta
     6481 ctcatccatt atccgcaacg atgtcgatta ccaatatttt cgcgactttg ccgaaaataa
     6541 aggcgcgttc gccgtaggtg caagcaatat ttccatccaa gacaagcaag gcaaaatatt
     6601 aggcagggtt ctcaacggca tccccatgcc cgacttccga gtcagcaacc ggcaaaccgc
     6661 catcgccacc ctgattcacc cccaatacgt caacagtgtc aaacacaacg tcggctacgg
     6721 ttccatacaa ttcggcaacg acacccaaaa tccagaagaa caagcctata cctaccgcct
     6781 cgtatcacgc aacccgcacc cggactacga ctaccacctt ccccgcctca acaaactggt
     6841 taccgaaatc tcacctgccg cactcagcag cgtacccttg cttggaaacg gccagccaaa
     6901 ggccaatgcc tacctcgata ccgaccgctt cccctacttt gtacgactcg gctcaggcac
     6961 gcaacaagtc cgcaaagcag acggcacgcg tacacgaacc gccccggcat accaatacct
     7021 gaccggcggc acgccgctga aagtattggg gttccaaaac cacggcttac tcgtcggcgg
     7081 cagcctgacc gaccaacccc ttaacaccta cgcaatcgcc ggagacagcg gttcccccct
     7141 gtttgccttc gacaagcatg aaaaccgctg ggtgcttgcg ggcgtactca gcacctacgc
     7201 cggcttcgat aatttcttca acaaatacat cgtcacgcaa cccgaattca tccgttccac
     7261 catccgccaa tacgaaaccc ggctggatgt cgggctgacc accaacgaac tcatatggcg
     7321 cgacaacggt aatggcgaca gcaccctgca agggctcaac gaacgcatca ccctgcccat
     7381 tgcaaaccct tcgcttgccc cacaaaacga cagcaggcac atgccgtctg aagatgccgg
     7441 caaaacgctc atcctatcca gcaggttcga caacaaaaca ctgatgctgg cagacaatat
     7501 caaccaaggc gcaggcgcat tgcagttcga cagcaacttc accgtcgtcg gtaaaaacca
     7561 cacatggcaa ggtgcaggcg ttatcgtagc cgacggcaaa cgcgtcttct ggcaagtcag
     7621 caaccccaaa ggcgaccggc tctccaaact gggcgcaggc acgcttatcg ccaacggaca
     7681 aggcatcaac cagggcgaca tcagcatcgg ggaaggcact gtcgtactcg cccaaaaagc
     7741 tgcttcagac ggcagcaaac aagcattcaa ccaagtcggc atcaccagcg gcaggggtac
     7801 ggccgtcctc gccgacagcc agcaaatcaa atccgaaaac ctctatttcg gcttcagggg
     7861 cggacggctc gacctcaacg gcaacaacct tgcctttacc catatccgcc atgcggacgg
     7921 cggcgcgcag atcgtcaacc acaaccctga ccaagccgcg acactgacgc tgaccggcaa
     7981 ccccgtcctc agtcctgagc atgtcgagtg ggtgcaatgg ggcaaccgtc cgcaaggcaa
     8041 cgcggcggtt tacgaataca tcaacccgca ccgcaaccgt cggaccgact acttcatact
     8101 caaacccggc ggcaacccgc gcgaattttt cccgttaaat atggaaaact caacaagctg
     8161 gcaatttatc ggcaacaaca ggcaacaggc cgccaaacaa gtcgcccaag ccgaaaatgc
     8221 ccgccccgac ctaattacct tcggcggata cttgggtgag aacgcgcaaa cgggcaaagc
     8281 cgcaccgagt tacagcaaaa ccaatatcga atgggtgaat ggtccaaaca ccccacacaa
     8341 gccctcgccc tatacgaata cgccaacccg cacaggggca ggcgtaccga cgattaccgc
     8401 ctcaaagaag gcggcaaccc cggcgcctac ttcccatgga atgcctacag cacccaagac
     8461 tgggaatttc tcggacatga ccggcgaagc agccatagaa aaaaaccgcc atatcgcaaa
     8521 tgccgccgta tacggccggc ccgaataccg ttacaacggc gcactcaacc tgcactatcg
     8581 tcccaaacgc accgacagca cgctgttgct caacggcggc atgaacctta acggggaagt
     8641 cttgattgag ggcggcaata tgattgtgtc aggcaggccc gtaccccatg cctacgacca
     8701 ccaggccaaa cgcgaacccg ttcttgaaaa cgaatggacc gacggcagct tcaaggctgc
     8761 acggttcacc ctacgaaacc atgcccgact gacagcaggg cgcaataccg cgcatctgga
     8821 cggcgacata accgcataca atctgtccgg catcgacctc ggctttatcc aaggcaaaac
     8881 accggaatgc taccgctcct accatagcgg cagcacccac tgcacaccca acgccgtttt
     8941 aaaagccgaa aactatcgtg cactacctgt aacgcaagta cgcggcgaca ttacccttaa
     9001 cgaccgttca gagctccgcc tgggcaaagc acacctgtac ggcagcatcc gtgccggcaa
     9061 agacaccgca gtccgcatgg aagcagacag caactggaca ctttcccagt ccagccacac
     9121 cggcgcactg acgcttgacg gcgcacaaat taccctgaac cccgatttcg ccaataatac
     9181 acacaacaac cgcttcaaca cactgaccgt caacggcaca cttgacgggt tcggcacatt
     9241 ccgattcctg accggcatcg tccgaaaaca aaatgccccc cccctcaaac tggaagggga
     9301 cagccgcggc gcattccaaa tccacgtcaa aaacaccgga caagaacctc aaacaaccga
     9361 atcgcttgca cttgtgagcc tcaatccgaa acacagccac caagcccgat tcaccctcca
     9421 aaacggctat gccgatttgg gtgcctaccg ctacatcctc cgcaaaaaca acaacggata
     9481 cagcctgtac aacccgctca aagaggccga acttcaaatt gaagccacgc gtgcggaaca
     9541 tgagcgcaac caacaggcat acaaccaatt acaggcaacc gacatcagca gacaggttca
     9601 acatgactct gacgcgacca ggcaggcact acaggcctgg cagaacagtc aaaccgaact
     9661 tgcccgcatc gacagccaag tccaatatct gtccgcccaa ttgaaacaga cagacccgct
     9721 gaccggcatt ctgacgcgtg cccaaaacct gtgtgccgca caaggataca gtgtcgatat
     9781 ctgccgtcag gttgccaaag ccgccgacac gaacgacctg acactcttcg aaaccgaact
     9841 ggatacgtat atagaacgtg tagaaatggc cgaatccgaa cttgacaaag cacggcaagg
     9901 cggcgatgcg caagccgtcg aaacagcccg gcacgcctac ctgaacgcac tcaaccgtct
     9961 gtcccgacaa atccacagtt tgaaaaccgg cgttgccggc atccgtatgc cgaacctggc
    10021 cgaactgatc agccggtcgg ccaacaccgc cgtttccgaa caggccgcct acaataccgg
    10081 ccggcaacag gcgggacgcc gcatcgaccg ccaccttacc gatccgcagc agcaaaacat
    10141 ctggctggaa accggtacgc aacaaaccga ctaccatagc ggcacacacc gtccctacca
    10201 acaaactacc aactatgcac atatcggcat ccaaaccggc atcaccgacc gtctcagtgt
    10261 cggtacgatt ttaaccgatg agcgcacaaa caaccgtttt gatgaaggcg tatccgcccg
    10321 aaaccgcagc aacggcgcac atctgttcgt caaaggggaa aacggcgcac tctttgccgc
    10381 ggcagattta ggctacagca acagccgtac ccgatttacc gattatgacg gggctgccgt
    10441 ccgccgccac gcatgggatg caggcatcaa caccggcatc aaaatcgata ccggcatcaa
    10501 cctcagaccc tatgccggca tccgtataaa ccgcagcaac ggcaaccggt acgtactcga
    10561 cggcgcagag ataaacagcc cggcgcaaat ccaaaccaca tggcatgccg gcatccgtct
    10621 cgataaaacc gtcgaactgg gtcaagccaa gctgaccccc gctttcagca gcgattacta
    10681 ccatacccgc caaaacagcg gttccgccct cagcgtcaac gaccgtacct tactgcagca
    10741 agccgcccac ggcacactgc ataccctgca aatcgacgcc ggatacaaag gctggaacgc
    10801 caaacttcat gccgcttacg gcaaagacag caacaccgcc cgccacaaac aggcaggaat
    10861 caaaataggc tacaactggt aacaagccga taaaaatgcc gtctggaacc cgcgtttcag
    10921 acggcatttg cgttaaaaat agtaaaccgt tccaaaaggg agtagaatag tgccgtttcc
    10981 aaccctgcgc ctgtaccgtc aggcttttat tatggacctt cccagttcgt ttttactgaa
    11041 cgccccttcc gattccaaac gacaataacc atcccgccgg aatgcctccc cgcacacgac
    11101 gggcggagca tttatgagca tcgaacacac tcctccgaca cacgacggcg aaaccggtca
    11161 aaaccatgcc gaacgccctt ccgctgattt cgaccgtgtc cactccctct gcgaaatcct
    11221 cgaacctgcc tttgaacaaa tcgaaaacgg cacaccgctc gaagacgcgc cgctgcgcga
    11281 caagctgacc gagctgaccg tcctcttgag cgagctgcac cctgccgacg tggcggcagt
    11341 gttggaatcg ctgccgccgc gcgaacgcaa tatcgtctgg attctggtca aatcggaaga
    11401 cgacggcgaa gtcttgctgg aagtgtccga tgcggtgcgc gaaacgctga tcgagtcgat
    11461 ggacaaagac gaattgttgg cggcggtcga tgatttggac gcggacgaat tggcggagct
    11521 ggcagacgat ttgccgcacc aagtggttta tgaagccttg cagacgcgcg acgaggaaga
    11581 gcgcgcacaa gtcaaagcgg caatgtccta cgaagacaac caagtcggtg cgattatgga
    11641 cttcgagttg gtcagcatcc gcgccgatgt cgcctgtgaa gtggtgctgc gctatctgcg
    11701 ccgcttcgac agcctgcccg accataccga caagattttt gtggtcgatg aaaacgacgt
    11761 actgcaaggc gtgctgccca tccgcaaact tttggtcgcc gatcccgaag atttggtgga
    11821 aaacgtgatg gcgaaagatg tcgtgcgttt ccgcgccgaa gacgacgtgg aagaagcagc
    11881 gcaggcattc gaacgctatg acttggttac cgcgcccgtc gtcgatgaaa acaaaaagct
    11941 catcggcagg gtgaccgttg acgaaatggt ggacgtgatc cgcgaagaat ccgaagcgga
    12001 tatgttgaac atggcaggtt tgcaggaaga ggaagacctg ttcgcccccg tcttggattc
    12061 cgtcaaaaac cgctggatgt ggctcgccgt caacctctgc accgccttcc tcgccagccg
    12121 cgtcatcggc gcgtttgaag gcagcatcga aaaaatcgtc gcactcgccg cgctgatgcc
    12181 catcgtcgcc ggcatcggcg gcaactcggg caaccagacg attaccatga ttgtccgcgc
    12241 gatggcgatg gggcaactga caggcatgca ggcaggcagg ctgttgaaaa aagaagtcgg
    12301 tgtcgccttg gtcaacggca tcatttgggg aacggtcatg ggcgcagtat cttggctgct
    12361 ttacggcagc ctcggcatcg ggctggttat gattgccgcg atgacgctca acctcctgct
    12421 ggcggcaacc gtcggcgtat taattcccgt ggtaatggaa aagttcggac gcgatcccgc
    12481 actgggcagc tcggtgctga ttaccgccgt taccgactcc ggcggcttcc tgattttctt
    12541 ggggctcgcc accctattcc tgctttaaat gccgtctgaa cccgcgcaaa aatgccgtct
    12601 gaagcggaag ctgcttcaga cggcatttga ctatttatcc ttgttgcaca agattattgg
    12661 acgatatgcc ggggcagccc tttggcaacg ccgaccacat cctccccgaa cagcgcgttg
    12721 acatcggttt cgtcaaacac atatttgctg tggcagaaat cgcaatcgac ttcgatgctg
    12781 ccttgttcca ccaccacgcc gccgacttct tccccgccca gcatcaacag catatcgctg
    12841 actttgccgc gcgaacaggt gcatgaaaat tcaaacgttt ccggctcgaa cacgcgcggc
    12901 ggcgtttcgt ggaacaagcg gtacaaaaca tgttgcgcat ccaattctgc caactcgccc
    12961 gccgtcagcg tgcgcgccag cgtactgacg tgttcccatg cctcttcatc caacacctct
    13021 tcaggcagac gctgcaccag cagaccgccc gccgcttcat cgcttgcaga caggacgatg
    13081 tgcgtatcaa gctgttcgga acgtttcata tagttcatca acatttgcgc gataccgtcg
    13141 ccttccaaag gcactacgcc ctgccagggt tcgccgtctt tgggctgcag cgtcagcacg
    13201 aatacgccgc cctcgcccaa aagatcgacc aaactttcat cttcaccgat ttctgccgcc
    13261 tcgtcccacc gcgcggtcgc acggacggta cggtcggaag tcgcttccgc aaccagcatt
    13321 ttcagccgcc cctgcccctg aacctgcaca atcagcgtgc cttcgttttt gaggttgccc
    13381 gacagcagaa caccggcagc caacagctcg cccaaggcgc ggcggatggc ggcgggatag
    13441 tttttctgtt ttacaatgtg ctgccactcg ttttccagac ggacgtgcag cccgcgcacg
    13501 ggcatatcgt cgaagataaa gcgggtacgc acatcggcgc ggttgatggc ggtttgattc
    13561 atgattttct ctgactgatt gttcggatgg cggctatatg gttgcggtcg gcgcgaaaac
    13621 aagacggacg gcggatgcgc ttcccaaatt atcaataaat tatataaaaa tcaacatatt
    13681 aactcaatct aacaagccgt tttttgccaa acagccgttt ttttatatac aatcaacaag
    13741 atattttcga ctgatacagc ataacatcgc acggcggcac gatgcctcct gcgcggaaac
    13801 accgatatgg attctttttt caaaccggca gtttgggcgg ttttgtggct gatgtttgcc
    13861 gtccgccccg cccttgccga cgagttgacc aacctgctca gcagccgcga gcagattctc
    13921 agacagtttg ccgaagacga acagcccgtt ttacccatca accgagcccc cgcccggcgg
    13981 gcgggcaatg ccgacgaact catcggcagc gcgatggggc ttaacgaaca gcccgtttta
    14041 cccgtcaacc gagtccccgc ccggcgggcg ggcaatgccg acgaactcat cggcaacgcg
    14101 atggggctta acgaacagcc cgttttaccc gtcaaccgag cccccgcccg gcgggcgggc
    14161 aatgccgacg aactcatcgg caacgcgatg gggcttttgg gtattgccta ccgctacggc
    14221 ggcacatcga tttctaccgg ttttgactgc agcggcttca tgcagcacat cttcaaacgc
    14281 gccatgggca tcaacctgcc gcgcacgtcg gcagaacagg cgcggatggg tacgccggtt
    14341 gcccgaagcg aattgcagcc cggggatatg gtgtttttcc gcacgctcgg cggcagccgc
    14401 atttcccatg tcggacttta tatcggcaac aaccgcttca tccacgcgcc gcgcacgggg
    14461 aaaaatatcg aaatcaccag cctgagccac aaatattgga gcggcaaata cgcgttcgcc
    14521 cgccgggtca agaaaaacga cccgtcccgc tttctgaact gatttcccaa ggaatacgca
    14581 atgagtatgc ccgaaatgcc caaatggtac gacgatgacg gacagatcgt gtcctgtacc
    14641 gaaaaggtca aagtgatgtc cgaaaatatg gccgagctgt atcagacggc acaagacgcg
    14701 tttgaagacg cgctgctgat gggttgcggc gaacgtcagt tgcgcgatta cctgctcgcg
    14761 ctgattgaag gtttggaaaa tccctaccgc aaagtctgaa cacgccccgg ttgctgcggc
    14821 acggtttatc cgtgccgttt ttgcgtttgt gcgcggcttc ggcttttcag acggcatatt
    14881 tgacgttatg attaaacagt taacaagatt tatcacaacg ccgtcaaaag acagacacac
    14941 aacatgaaca tcatccgcgc gctcctcatc atcctcggct gcctcgccgc cggcgaaacc
    15001 gccgttttcc tagcaggcat caaactgccc gacagcatcg tcggcatggg cgtgctgttt
    15061 gcgcttttgc aggcaggttg ggtcaaaacg tcttggctgc aacagcttac cgacgcgctg
    15121 atggcgaatc tgacgctgtt tctcgtgccg ccctgcgtgg cggtcatcag ctatttggat
    15181 ttgattgccg acgattggtt ttcgatactg gtttccgcct ccgccagcac tttgtgcgta
    15241 ctgctggtta caggcaaggt tcaccgctgg atacggagca ttatctgatg aacgaaatcc
    15301 tcaggcagcc cagcgttctg cttttcctca cgcttgccgt gtacgcgctt gcgattatcg
    15361 tgcgcacgcg cacgggcaat atcttctgca accccgtact cgtcagcact atcgtgctga
    15421 ttgcctacct gaaaatcctc gacatcgatt atgcggtgta ccacaacgcc gcgcaattca
    15481 ttgatttttg gctgaaaccc gccgtcatcg tgcttgccgt gccgctctac caaaaccgcc
    15541 gtaaaatctt caaccagtgg ctgcccgtca tcgtttcaca gcttgcgggc agcgttacgg
    15601 gcattgttac ggggatgtat tttgccaaat ggctgggcgc ggaacgcgaa gtcgtcctct
    15661 cgctcgcgtc caaatctgtt accaacccca tcgccattga aatcacccgc tccatcggcg
    15721 gcattcccgc cattaccgcc gccaccgtca tcattgccgg tctggtcgga cagattgccg
    15781 gttacaaaat gctgaagaac acagtcgtca tgccctcgtc cgtgggtatg tcgctcggca
    15841 cggcttcgca cgcgatgggg attgccgcct cgctcgaacg cagccgccgc atggcggcat
    15901 acgcgggact ggggctgacg ttcaacggcg tactgaccgc gctgattgcg ccgctgctca
    15961 tccccgtttt gggattttga acccgtttca gacggcattt cagcccatgc tgtctgaacg
    16021 ccgacacact cgcaaggaga accgttatgg ctgtcaacct gactgaaaaa accgccgaac
    16081 aactgcccga catcgacggc attgccctct acaccgccca agcaggcgtg aagaagcccg
    16141 ggcataccga cctgacactg attgccgtag ccgccggcag caccgtcggt gcagtcttca
    16201 cgaccaaccg tttctgtgcc gcgcccgtcc acatcgccaa atcgcacctt ttcgacgaag
    16261 acggcgtgcg cgccctcatc atcaacacgg gcaacgccaa cgcgggtacg ggcgcacagg
    16321 gcagaatcga tgctttggca gtgtgtgccg ccgccgcccg gcaaatcggc tgcaaaccga
    16381 accaggtgct gcccttctcc accggcgtga ttctcgaacc gctgcccgca gacaaaatca
    16441 tcgccgcgct gcccaaaatg cagcctgcct tctggaacga agcggcacgc gccatcatga
    16501 ccaccgacac cgtgcccaaa gccgcctcgc gcgaaggcaa ggtcggcgac aaacacaccg
    16561 tccgcgccac gggcatcgcc aaaggctcgg gcatgattca tcccaatatg gcgaccatgc
    16621 tcggtttcat cgccaccgat gccaaagttt cccaacccgt cctccaactg atgacgcagg
    16681 aaatcgccga cgaaaccttc aacaccatca ccgttgacgg cgacaccagc accaacgaca
    16741 gcttcgtcat catcgccacc ggcaaaaaca gccaaagcga aatcgacaac atcgccgacc
    16801 cgcgttacgc ccaactcaaa gaattgttgt gcagcctcgc gctcgaactc gcccaagcca
    16861 tcgtccgcga cggcgaaggt gcgaccaagt tcatcaccgt ccgcgtcgaa aacgccaaaa
    16921 cccgcgacga agcccgccaa gccgcctacg ccgtggcacg ttcgccgctg gtcaaaaccg
    16981 cctttttcgc ctccgacccc aacctcggca ggctgctcgc cgccatcggt tatgccggcg
    17041 ttgtcgacct cgatactgac ctcgtggaaa tgtatctcga cgatattttg gttgccgaac
    17101 acggtggacg cgccgcaagc tacaccgaag cacaagggca ggcggtgatg tcgaaggccg
    17161 aaatcaccgt ccgcatcaag ctgcatcgcg gacaaagtgc cgccaccgtc tatacctgcg
    17221 acctgtcgca cggatacgtt tccatcaacg ccgactaccg ttcctgaccc gacacggctt
    17281 cagacggcat acataaaatg ccgtctgaac cgccggacaa catgccatga cctccacatt
    17341 cccccgccgc ctcgcccgca aaatccgcca aacccgccgc ctgtcgcgca aaagcatcgc
    17401 ctttctgttc cttttggcag gttcggcact cgtcgccctg accgcgctgt tttttgccca
    17461 tcttgccgat tttgcgctgg aactgaacgc caaactggtt caacaatacc cgtggttcgc
    17521 gtgggtcgcg cttcctttgg gtttgccgct tattgcgtgg ctcacacgca aattcgcccc
    17581 cttcaccgcc ggcagcggca tcccgcaggt catcgcctca ctgtcgctgc cctacggcgc
    17641 acagaaaacg cggctgatcc gcctcgggca gacgctgctg aagattccgc tgaccttttt
    17701 gggtatgctg ttcggcgtgt ccatcggacg cgaaggtccg tccgtgcagg tcggcgcggc
    17761 agtgatgggc gcgtggggcg cgtggtgcaa gaaacacggc ttggcattca aagggatgca
    17821 ggaaaacgat ttgatggcgg cgggcgcggc gggcggtttg gcagccgcgt tcaacgcgcc
    17881 gctggcgggc gtgattttcg ccattgagga actcgggcgc ggcatcatgt tgcgctggga
    17941 gaggcaaatt cttttgggcg tgctcgcctc cggtttcata caggtcgcca ttcagggcaa
    18001 caacccgtat ttttccggct tcaacggcgg cgtattggaa catatctttc tgtgggtcgc
    18061 actgtccggc ctggtttgcg gcgcggcggg cggcctgttc ggacgtttgc tctatcgcgg
    18121 tgcggcggcg tttgcaccgc gcaagatacg cggcttcatc cgcaaccgtc cgctgctgct
    18181 ggcggcactg atggggctgc tgctcgccct gctcggcacg ttctaccaag gcaaaaccta
    18241 cggcaccggc taccacgaag ccgcccaagc cctgcacggc atctacgaag cccccttcgg
    18301 actcgccgcc gccaaatggc tcgccaccgt attcagctat tgggcaggcg ttccgggcgg
    18361 cattttcact ccctcgctga ccataggcgc ggttttgggc gagcatatcg ccgccatcgc
    18421 cgacatatcg cagggtgcaa acatcatcgt cctcatctgc atggcggcat ttctggcggg
    18481 cgcgacacaa tccccgatta cttccgccgt cgtcgtcatg gaaatgacgg gcggacaaag
    18541 cctgctgttt tggatgctaa ttgcctgcat tttcgcctcg caggtttcgc gccagttttc
    18601 gccgcgtccg ttctaccacg catcgggaat gcgcttccgc cagcgcgtgc ttcaagaaac
    18661 cgccgcccaa accggcaatg cgcccgcaag accgcaaaca gcaaacagca aaacgggaat
    18721 gccgtctgaa aattaaaacg cccccgatca aacgccggca gccgccttga tttgaatacc
    18781 gttccgccgc cgcttgaaat ttcagcaaca atgccgtctg aacgacagaa tgcggttttc
    18841 agacggcatt tccccatccc gatattgcct aaacaaaacc gaagcgtttg ctataattgc
    18901 actttttacc gcacacgcac gcccatgttt cccgatttct cccaaaccct ctccaaagac
    18961 cgccacttcc tgcaatccgc cttcaaaaat cccaacaaat acggcggctt gtccaaagtc
    19021 gaagaaaaat accgaaaatc gcacgaaatc tttttgaaac gtttggcagc cttgcccaaa
    19081 cccgaattcg acaacaccct gcccgttcac gaaaaacttg acgaaatcaa aaaaaccgtt
    19141 gccgaacatc aggtaacgat tatttgcggc gaaaccggtt cgggcaaaac cacgcagttg
    19201 cccaagattt gtttggaact cgggcgcggg gtagcgggtt tgatcgggca tacccagccg
    19261 cgccgtttgg cggcgcgttc ggtggcagag cggattgccg aagagctgaa atccgaaatc
    19321 ggcagcgcgg tcggctataa agtacgcttc accgaccaca cctcgcgcga tgcctgcgtc
    19381 aagctgatga ccgacggcat cctgctggcg gaaacgcaga ccgaccgtta tctcgccgcc
    19441 tacgacacga ttatcatcga cgaagcgcac gagcgcagcc tgaacatcga cttccttttg
    19501 ggctatttga aacaactcct gccgcgccgc cccgatttga aagtcatcat cacctcggca
    19561 acgatagacg cagaacgctt ctcccgacac ttcaacggcg cgcccgtttt agaagtgagc
    19621 ggacggacgt atcccgtcga aatcctctac cgaccgctga ccggcaaaga cgaagacgac
    19681 gcagaagtgg agttgaccga cgcgattgtc gatgcggcgg acgaattagc gcgacacggc
    19741 gaaggcgata ttttggtatt cctgccgggc gagcgcgaaa tccgcgaaac tgccgaagcc
    19801 ttacgcaaat ctacactgcg ccgcaacgac gaaatccttc ccctgttcgc acgcctgtcg
    19861 cacgccgagc agcacaaaat cttccaccct tcaggcgcga aacgccgcat cgtattggca
    19921 accaacgtcg ccgaaacctc gcttaccgtg ccgggcatca aatacgtcat cgacaccggc
    19981 ctcgcgcgcg tcaaacgcta ttccgcacgg gcaaaggtgg agcagcttca tgtcgaaaaa
    20041 atctctcaag ccgccgcccg ccaacgctcc ggccgctgcg gacgcgtctc cgcaggcgtg
    20101 tgtatcagac tgttttcaga agaagatttc aacagccgcc ccgaatttac cgacccagaa
    20161 atcgtccgca gcaacctcgc cgccgtcatc ctgcgcatgg catcgctgaa cctgggcgat
    20221 gtggcggcat tcccattttt agaaatgccc gattcgcggt atatcaatga cggttttcag
    20281 gtattgctgg aattgggggc ggtagaggcc gtctgaaaac aagtaggata tacacttcta
    20341 cctgacaaag accaacaaac gataaaacta cggcacatct agatgcccaa cctacaacat
    20401 tgcacacggt aaaaacatga aacacttact catcgatttt gaaaacgtcc agccgcaaaa
    20461 cttagacaaa ttaccaaccg aaaataccca tatttggcta tttataggtg tattacacaa
    20521 aatgttacct attagtctgg tgcaatccct actacgtttc ggcgaacgtg tccatcttgt
    20581 ccagttacaa aaaacgggga aaaacgcatt ggatttttac ctgtcctatt acctcggaca
    20641 aattaccgcc acagacccca atgcccaaat cggcatactc tcgcgtgatg gaggatacga
    20701 tgttctggtc gaacatattt tgaaaaacca ccaggcgaag ggtatcgtgc gcctagccaa
    20761 tatagatgaa gtacaacatc agaaaattac taccgaaccg ccgtcagcat tgctggaaaa
    20821 cactcctcag cctgaaacca ccctcaaacc acagcaacca ttaacttcct atttccaagc
    20881 agccctaact gcactgcgcc gccccgacgc tttccgcccc tgccgcctgc ataacctgcg
    20941 acaaaatctg cgtaagcata ttttgagtga tttgtttaaa gaaaaaaccg atgaagaatg
    21001 cgaaataacc actgctaacg ttatcaataa actcaaagca caaaacttca tcagcattga
    21061 tgaacaggaa accgtttcct accatctcag tgataatgat ttgttacaaa gaatccaacg
    21121 ccatatttta agccaacgtc ccaaaaccta cgctgatttt caagccgtcg tgcaaaaccg
    21181 agcagatgca cttcacttaa cagtcggtac caacgacatt caatcctttg cgcgacattt
    21241 gcgcgaccaa aacctgatcc gccaaaacaa tgggaaaatt gaatatgcac cgtttactga
    21301 acctaaacca cagccaacgc ccaagcagcc taaaaaaacc gcatgggaac ctgatgaaat
    21361 tatttggaaa aaagtgattg ccgcgttatc gttaaagaac cgtcctaata aaaccaaaac
    21421 tttacgcaat acaatccagg cactcacaaa atccaatgca caagaaactg acaaactgct
    21481 acaacattta caagataccc aagtcctacg tattgacggt actaaaattg tgtatatcaa
    21541 gtaggatggg catttatgcc tgacctacaa aaccaaaaac aacctaccac ccttaatcaa
    21601 ctccacaaac ccttttcaga caacctcgtt ttttgaaaaa caatctgtaa acagatagct
    21661 gctgaagaat accgttgccg agccccaaaa cccgtcctgc aacttttatt gtgaacttcc
    21721 cattatgaaa aaatcccttt tcgttctctt tctgtattcg tccctactta ctgccagcga
    21781 aattgcttat cgctttgtat tcggaattga aaccttaccg gctgcaaaaa tggcagaaac
    21841 gtttgcgctg acatttgtga ttgctgcgct gtatctgttt gcgcgttata aggcgacgcg
    21901 tttgttgatt gcggtgtttt tcgcgttcag cattattgcc aacaatgtgc attacgcggt
    21961 ttatcaaagt tggatgacgg gcatcaatta ttggctgatg ctgaaagaga ttaccgaagt
    22021 tggcggcgcg ggggcgtcga tgttggataa gttgtggctg cctgcgttgt ggggcatgtt
    22081 ggaagtcatg ttgttttgca gccttgccaa gttccgccgt aagacgcatt tttctgccga
    22141 tatactgttt gccttcctaa tgctgatgat tttcgtgcgt tcgttcgaca cgaaacaaga
    22201 gcacggtatt tcgcccaaac cgacatacag ccgcatcaaa gccaattatt tcagcttcgg
    22261 ttattttgtc ggacgcgtgt tgccgtatca gttgtttgat ttaagcagga ttcccgcctt
    22321 taagcagcct gctccaagca aaatcgggca gggcagtgtt caaaatatcg tcctgattat
    22381 gggcgaaagc gaaagcgcgg cgcatttgaa gctgtttggc tacggacgcg aaacttcgcc
    22441 gtttttaacc cggctgtcgc aagccgattt taagccgatt gtgaaacaaa gttattccgc
    22501 aggctttatg actgcagtgt ccctgcccag ttttttcaat gcgataccgc acgccaacgg
    22561 cttggaacaa atcagcggcg gcgatactaa tatgttccgc ctcgccaaag agcagggcta
    22621 tgagacgtat ttttacagcg cgcaggcgga aaacgagatg gcgattttga acttaatcgg
    22681 taagaaatgg atagaccatc tgattcagcc gacgcagctt ggctacggca acggcgacaa
    22741 tatgcccgat gagaagctgc tgccgctgct cgacaaaatc aatttgcagc agggcaggca
    22801 ttttatcgtg ttgcaccaac gcggttcgca cgccccatac ggcgcattgt tgcagcctca
    22861 agataaagta ttcggcgaag ccgatattgt ggataagtac gacaacacca tccacaaaac
    22921 cgaccaaatg attcaaaccg tattcgagca gctgcaaaag cagcctgacg gcaactggct
    22981 gtttgcctat acctccgatc atggccagta tgttcgccaa gatatctaca atcaaggcac
    23041 ggtgcagccc gacgctatct cgtgccgctg gtgttgtaca gcccggataa ggccgtgcaa
    23101 caggctgcca accaggcttt tgcaccttgc gagattgcct tccatcagca gctttcaacg
    23161 ttcctgattc acacgttggg ctacgatatg ccggtttcag gttgtcgcga aggctcggta
    23221 acgggcaacc tgattacggg tgatgcaggc agcttgaaca ttcgcgacgg caaggcggaa
    23281 tatgtttatc cgcaatgagt ggcgtaaaaa ccaataaaga caaatttaga tgatgtcggg
    23341 gaagatgccc gaccgacaag actatgcaaa atatgaaaaa ccaagtacgc ggatcaggca
    23401 tggatgcccg atccaacccg gccaatgttt cagacgacct gcaaaacagt tcgggccata
    23461 tcggtgtcaa cacgcgttac cgcctgacca aactcggcga acaaatggcg cgcctgccca
    23521 tcgacccgaa aatcgcgcgc attttgctgg cggcgaagaa acacgactgc atggcggaaa
    23581 tattggtgat agcgtccgcg ctgtcgattc aagacccgcg cgagcggccg ttggaagcgc
    23641 gcgatgccgc cgccaaggca cacgagcgtt ttaccgacaa gcaatccgac ttccttgcct
    23701 atctgaacat ttgggacagc ttccagcgcg agcgcgacaa aggtttgtcc aacaagcagc
    23761 tggtgcagtg gtgccgtcaa tatttcctgt cgcacctgag gatgcgcgag tggcgcgagc
    23821 tgcaccacca gcttgcccaa accgcgattg aaatgggttt gaccacgaaa gaagccgctt
    23881 tcagacggcc tcccgaaggc aggcagctta cgtcgtctga aaatgcgggt gaccaagact
    23941 tatccgccaa gctcaaacaa aaacaactgg ataaaaagca acaccgcgcc caaatccgcg
    24001 ccgccaaaga agcgggctac gaacaaatcc accgcgccct gctcaccggc cttatcgcca
    24061 acgtcggcat gaaatcgccc gacggcaacg actacaccgg cgcgcgcggc agccgcttcc
    24121 accttttccc cgcctccgcc ctgttcaaag ccaagcccaa atgggtgatg gcggcagaat
    24181 tggttgaaac cacgcgcctt tacgcgcgcg acgtcgccgt tatccagccc gaatggatag
    24241 agcaggaagc gccgcacctc gtccgctatc attatttcga gccgcattgg gaacaaaaac
    24301 gcggcgaagt cgtcgccagc gaacgcgtga cgctttacgg tctgaccgta ttgccgcgcc
    24361 gccccgtgtc ttacggcaaa gttgcccccg aagaagcgcg cgaaatcttt atccgcagcg
    24421 cgttggtggc gcaggaatgc gatttgaaag cggatttttt tgtccacaac aaaaagctga
    24481 ttaaagaaat taccgaactc gaacacaaat cgcgcaagca agacgtgctg gtcgatgacg
    24541 aagccctgtt tgcgttttat aacgaacggc tgcccgattt ttatacggcg gatgcggttt
    24601 cagacgacct gcatcctaca aatccgcagc aaactgcccc ctcctacgcg agggaagaac
    24661 ggagagaggg caaaacagtt gccgcacaaa ccaacttctc cgcaaccgca gcaaaccctc
    24721 tccctaaccc tctcccacag gagagggagc agagtgccgc agcttcagcg gtttcaaacg
    24781 acctgcatcc tgcaaatccc caacaaaccg ccccctcccc cgtgggggag ggtcggggag
    24841 agggcaaaac agttgcctct caaaccaact tctccgcaac cacagcaaac cctctcccta
    24901 accctctccc taaccctctc ccacaggagg gggaacagag tgccgcagct tcagcggttt
    24961 caaacgaccc tcaaccccaa aagcagccag caccccaaaa agaccgtctg aaacccctcc
    25021 ccctcgccga catccgcacc ttccaagtct ggctcaaaac cgccgaacgc gagaacccgc
    25081 gcctgctgtt cctcagccgc gacgatttga tgcaacacgc cgccgcacac attaccgaag
    25141 agcaattccc caaattctgg caaaccgcag acggcaagtt caaactttcc taccgcttcg
    25201 agccgcacca tccgctcgac ggcgttaccc tcaccctacc gctgaccgtc ctcaaccgcc
    25261 tgcacgcgcc gtcgctcgaa tggctggtgc ccggtatgtt gcgcgaaaaa atccagctgc
    25321 tgattaaagc tctgccgaag caaatccgcc gcatttgcgt gcccgtaccc gatttcatca
    25381 cccaattttt aagccaaaac cctgatcgca acgcccccat cctgccccaa cttgcccaag
    25441 ccatcgccaa aaccgcaggc gacatccgca tactcgagca aatcaaccaa gacgaatggg
    25501 ccgcgttcag gctgcccgaa cactgctatt tcaacctccg cattatcgac gacggcggac
    25561 aagagcttgc cggcggccgc aaactgcatg aattgcaaca acagctcggc caagccgccg
    25621 ccgttacctt ccgcgacaac acccaagaat tcgagcgcga caacgtgacc acatgggaca
    25681 ttggcaccct gcccgaatcc atcaaattcg cacgcggcaa acaacagctc accggctacc
    25741 tcggtctgca aaaagaaaaa gacggccgca tcgccctgcg cctgtgtgac accaccgaag
    25801 ctgccgaaca ggcacaccgt caaggcgtaa tcgaattgat gaagctgcaa ttaaaagagc
    25861 aggtaaaaga tctgaacaaa ggcattcaag gctttaccca agccgccatg ctgctcaaac
    25921 acatcaacgc cgacaccctg tgcgacgacc tcacccaagc cgtctgcgac cgcgccttta
    25981 tcggcgaaga cgagctgccg cgcaacgaaa aagcctttaa agagcaaatc aaacgcgccc
    26041 gcagccgcct gcctgccgtc aaagaagccc tcagccgcta cctgcaggaa accgcagccg
    26101 cctacgccga actcaacagc aaactcggca aacacccatt gacccacctc ctacgccaac
    26161 gcctgcaaac cctgctcgcc gccggcttcg ccacccgcac cccgtgggca caatggccgc
    26221 gcctccccat ctacctcaaa gccatgaccc tgcgcctcga aaaatacagc agcaaccccg
    26281 cccgcgacgc agcccgcgaa gccgatatcc aagagctgga acaaatgtgg caggaaaaaa
    26341 cagacagcct gattaaacaa ggtctcccca tttcagacgg cctcgccgcg tttaaatgga
    26401 tgattgaaga attgagggtg tcgctgttcg cgcaggaatt gaagacaccg tatccggtgt
    26461 cggtgaagcg gttgttgaag atgtgggagg atcttaatta aaggaagatg ccgtctgatt
    26521 ttttcagacg gcatctttta tttccctgac caaccgattt aatcgctccc taccaatagc
    26581 tttatttttt aattcgcact cccaaacgac aatcactttg aaaccgatct tttccagctc
    26641 ggtttcattt ttaatatcac gttcgcaatt ttttgtgatt ttttccaacc aaaaatccgt
    26701 attgcttttc gggatatgtc ctttattaca agaatgcccg taccaaaaac agccgtgtat
    26761 aaacacgact gttttatatt tcggcaaaac gatatccggt ttgcctgcat agcgtttatc
    26821 gtttttccga taacgaaaac cttgggagaa aagaaatttc cttaccaata tttccggttt
    26881 ggtttctttg ctatggattt ttgacatcac gaaagaacgt ttggatgtag tgaaaatatc
    26941 ggtcataaat tacttggaag ataccacctt cgatcgtttt tttgtctgtt ccacttttct
    27001 taacaaatac gaaaccaagc ttattaaatc caaagcatcc ttatcattaa aaatactagg
    27061 agataaattt gctcttaact catgttgtac aggatttcta aatccagtcc atgttccgca
    27121 tgcaagctgt tcaagaccat cttctaaatt ttgttccgcc ttcgttttgt tattcgttaa
    27181 tagaagtata gaatttttac tcccgaaagc ttgttcaatc aattctcttc cgtctatgct
    27241 atgaagacct gtttgcttct gaaccttatt ttcgaaaact ttaactgcct cgattgcagc
    27301 acttaaataa tccttctttt cattgtaaaa atcattacag gcagtatgca aatcctgatg
    27361 caaatgccgc caatgatagt aaggatattc tggaataata ctatgtaagg cagagattac
    27421 ttccgcttgc tctttatttg taagctccga atcttccaaa cgattcaaaa taacttctac
    27481 tggttccctt tccttttctg gcaatttgtt tttccaattt tcgatatcaa ccccgctttc
    27541 acctttcact tcgtcatctt tttctttttt tcttcttttt ctccaatctt gcccgatggc
    27601 tcgaacgatt tccccaagga aaacccgtaa ctttgaagtt ttaggatgct tccaatccaa
    27661 tgatgtccta tctgtagcaa ttaaatcttc atcatcatta tctataaaat caatacttaa
    27721 ccatcctgtc agataggaat agaaatggct ggattcgcta tcagtgaaaa actcgggcat
    27781 atttaccatt ctgccgttgg caaacaaggt aatacctttc atattattct ttaaaggttt
    27841 ttccgttgta atgaacttac cttgtattaa tccgctgtat tcatactgtt cgaattttga
    27901 agataacgat gaaatattgg ttgctagatc ctggtattcc cattcgaatt gtggggtaac
    27961 aagatcatat tttagatttc catcaatgat tttgggttcc gagccgttca agcttacttt
    28021 gattttaaaa tcctgagccg gaaaatcaaa caagcgggaa agatgctcca cataattatc
    28081 taacgggtat ccttgttttt tcgtcagttc ggttaaagtt atggttgttc cgttttcggt
    28141 attgggttca acagattctt tctgaaactc cggttgataa atacgctcac tttttttaat
    28201 ctctgcataa tccaaagtaa aagtaacccg ttcgtttcct tggatagtag aaatttcaat
    28261 tttgttgcca agcccgaata atgccaattt accaaggcct tttttacccg ttggaattct
    28321 tccgcacggg gaggcttgtt tttcttccct tctgttccga ccgattctca aataaaaatc
    28381 attgatttca tcgaagctca ttcctatacc attatcttta ataacgattt tatgctccgg
    28441 tttatcgaat aacctaactt ccacttccgt agcacatgca tcgtaggcat tcgctatcaa
    28501 ctcggcaatc gcaggaacag tgtgcgaata catctttaca cccaaatgtt cgattacggt
    28561 tggctcgaaa ctcattttca atttttcttc agacatgttt tctccttttc taacattcga
    28621 tattatcaac aattgccctg cctatttttt ccgcatacat cggaggaacg gcattaccaa
    28681 tcaaacgggc tatcttgtcc ctgctgcccg ctttaaagac atagttacga gggaacgatt
    28741 gcaatgttgc accttcgcgc agtgataacg ccctatcttc ttcaggatgg gcaaacctcc
    28801 cgttggaaat actaaagaat ttggtcgtta tggtaggtgc gggcttatcc caccatagcc
    28861 taccaaacgt gtctttaaaa ctattgtctt ttccgataaa acattccagc tgtaattcgg
    28921 gaatatgcgc aaaagccaaa cggtttcctc cgtttttagg tatcaaagcc aatcgtttca
    28981 agttgatatc agataatccc gcacagctat gcataaaatc cgtttcgtct tggtgtcctg
    29041 caataatttt gggaaagcca ttttccattc ccaaaacatc gcgtaccgta agccgtttgc
    29101 ccgaatactt gactggttcc agcttttctt tggttattct gtttgcaatt aacgtaaatc
    29161 ttttacggct ttggggaatt ccgtaatcag ccgtattgtg aataccgaaa tggacggtaa
    29221 atcctttttc ttccaataac tttatgaaat tatcaagcct gctttctttc attctgctca
    29281 gaattcccgg tacgttctct acaacaacat atccgggatt gaaatattct acaaaccgct
    29341 gaaactccaa aagcaaactc ttggattttt ccgattttct tttgtctgtt tgaataacac
    29401 tccaatattg acaaggacta caacctataa gaatcaaatc atcatcattc ttttttaacc
    29461 cgagttcttt ttccaaggtt tcaggctgca attcaaaaac atcagcctgt ataaatttgg
    29521 catttatatt ggcttcatat gtttctttgc agctgggttc gtaatctatc ccggctaaaa
    29581 cctgaatacc ggcggattgc attccatagc tcattccacc tccgccacag aaaaaatcaa
    29641 ccgcctttaa cttactgttt ttcatttcta tttaaataat tagactagtg agaactaact
    29701 ttatataaca tataatttat tgcttatcaa aaataatttg agattttatt aaaattcatg
    29761 catctaaata agcagaactt aatttcctcc aaacgagcgg atgtcttcaa atgcacactt
    29821 cacagaaagg tcgtctgaaa cccattttca gacgaccttt ttccacttcc caaaacaaga
    29881 attcccaccc cctccccaaa cgtcgcccga accagtaaac tccccatatt tcaacaactt
    29941 cttggaagcc aaccatgccc cgtatccacc ctcctcacct gttcccgccc catatcgccg
    30001 aacgcggcct tttgtatttt cagcagggca aggttcgcga tgtccgaaaa acttccgccg
    30061 ggcgttatcg
//
LOCUS       DAWKST010000003        27652 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.003, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000003 DAWKST010000000
VERSION     DAWKST010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27652
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.003"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(18..1271)
                     /locus_tag="WJL87_000072"
     CDS             complement(18..1271)
                     /locus_tag="WJL87_000072"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEZ7798569.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYRFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWFALLEAANQADSGKIAAECRAQGKAHLIAEQI
                     DRARLAQIAPLQKTFRAAQDKTEKH"
     gene            complement(1343..2971)
                     /locus_tag="WJL87_000073"
     CDS             complement(1343..2971)
                     /locus_tag="WJL87_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ7798570.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQDGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            3121..3495
                     /locus_tag="WJL87_000074"
     CDS             3121..3495
                     /locus_tag="WJL87_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ7798571.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQRPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAGTQQKVLSSTAEAVKLMSEMPSLIKRPVLECGGRV
                     YVGFSEETYDGIFNRQALCRQG"
     gene            3550..5088
                     /locus_tag="WJL87_000075"
     CDS             3550..5088
                     /locus_tag="WJL87_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ7798572.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAMQNEQADGGSPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPKAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            5155..5772
                     /gene="recR"
                     /locus_tag="WJL87_000076"
     CDS             5155..5772
                     /gene="recR"
                     /locus_tag="WJL87_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ7798573.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(5858..6136)
                     /locus_tag="WJL87_000077"
     CDS             complement(5858..6136)
                     /locus_tag="WJL87_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798574.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSDSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            6341..7588
                     /locus_tag="WJL87_000078"
     CDS             6341..7588
                     /locus_tag="WJL87_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEZ7798575.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDAVWVRDWTYNNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPGGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSWRASKTQPAEALRYE"
     gene            7581..8276
                     /gene="lolD"
                     /locus_tag="WJL87_000079"
     CDS             7581..8276
                     /gene="lolD"
                     /locus_tag="WJL87_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ7798576.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEIRAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLSQRQLGILRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKKREEAAETAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
     gene            8344..10089
                     /gene="recD"
                     /locus_tag="WJL87_000080"
     CDS             8344..10089
                     /gene="recD"
                     /locus_tag="WJL87_000080"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ7798577.1"
                     /translation="MERQTDEFAQAAARAAIWFLARYAGSGSEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     DTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQPFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFDPVYPLPFDVLVIDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGEAHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNKAYCSHVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPDFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEETFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(10117..11220)
                     /locus_tag="WJL87_000081"
     CDS             complement(10117..11220)
                     /locus_tag="WJL87_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7798578.1"
                     /translation="MSSFIFPDNGAEWRNESVQKPPKGWHYVRESGADGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKRARKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLGLRPADFLEKAFIRAGLRVADMM
                     KTKPKHKKAANPSDPLAFARTRETTFLYRLKKA"
     gene            11471..13921
                     /gene="lon"
                     /locus_tag="WJL87_000082"
     CDS             11471..13921
                     /gene="lon"
                     /locus_tag="WJL87_000082"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ7798579.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAALEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDTDHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DSKTKKTGKAKPRAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVGGELL
                     TVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIHIHVP
                     EGATPKDGPSAGIAMTLAAVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKLLAAL
                     RGGIKHVLIPKDNLKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEHSGAE
                     AAAESASKPKPRSRATKH"
     gene            14103..14372
                     /locus_tag="WJL87_000083"
     CDS             14103..14372
                     /locus_tag="WJL87_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ7798580.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(14431..14865)
                     /locus_tag="WJL87_000084"
     CDS             complement(14431..14865)
                     /locus_tag="WJL87_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798581.1"
                     /translation="MTEPKHEMPTEEQVAARKKAKAKIRTIRIWAWVILALLASTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLTVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(14858..16165)
                     /locus_tag="WJL87_000085"
     CDS             complement(14858..16165)
                     /locus_tag="WJL87_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ7798582.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREVRISAVCD
                     LSEEKARQICPSAAFVKDPFELVARKDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEEKNVMVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGHDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYCIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            16339..16734
                     /locus_tag="WJL87_000086"
     CDS             16339..16734
                     /locus_tag="WJL87_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798583.1"
                     /translation="MKRQQDKKMLFEENPIDEHFAEYDRRTGEIRLSGRHFNKPVLLH
                     RDSAGLSKWQSLSELTPDNLLSDVKADDYPEILIIGTGAAQEFIHPKIMADFSRIGIG
                     VECMNTDSAFRTLVFLHSEGRRAWAWLQP"
     gene            16811..18733
                     /locus_tag="WJL87_000087"
     CDS             16811..18733
                     /locus_tag="WJL87_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ7798584.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGKITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERVAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPKVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIIVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSQLAKVKTQLAQIEEVWLACQE
                     ELERIETEIEKQFAER"
     gene            18720..19133
                     /locus_tag="WJL87_000088"
     CDS             18720..19133
                     /locus_tag="WJL87_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ7798585.1"
                     /translation="MPSDKEARAGCLSLPANNGIIRALSPPFTGINIRLFAALGLLSL
                     SGAAAQASVYHCNSNGKSVYTSAPAGSCADADLPKISSHQGGGYRLKIKKLSQEAKIH
                     IESKKKNKKPAGKKNRQAAKAPKENQKQPTEKKSP"
     gene            19199..19714
                     /locus_tag="WJL87_000089"
     CDS             19199..19714
                     /locus_tag="WJL87_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798586.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYIPPQTPEPVSSPSNGGQVVKYKAPVKTVSKPAKSNTPPPQQ
                     APSNNSRRSILETELSNERKALVEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(20644..21747)
                     /locus_tag="WJL87_000090"
     CDS             complement(20644..21747)
                     /locus_tag="WJL87_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ7798587.1"
                     /translation="MITLITGVPGSGKTLSVVSDLAKKINNEWKDRKIFIEGIPELTI
                     ETTPIPEGHSINDMHVWLQYPENNGSVVIIDEAQNVFPPRSPAVKAPPLVEWLHVHRH
                     SGIDIILITQMPQRIDKHVRDLVGAHYHIHKTPLGIFMRYFWDYCANNPRSEFANARP
                     EVYKLDKKAFGLYKSAEIHTKVKTPKSRVLYIIPIALVLFALFFYLGYKLLLGLGNKD
                     GTEEGEAKTQTIVSEEQLEQVRGMAYKASGEAGRNIGNQLGSDKENQNLTKEMLEPTI
                     DGHPESKPIYNGVRQVRQMEFPVACVKSSNSCNCYSGQATILKEIDKKTCIDYVDNGM
                     PFNPYKEEQREIKIDTDEQKPLKKQVLVMDAAK"
     gene            complement(21758..22048)
                     /locus_tag="WJL87_000091"
     CDS             complement(21758..22048)
                     /locus_tag="WJL87_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HEZ7798588.1"
                     /translation="MPRLIVALFMALVPMLKDLFFKILAGLGASFVSYEALSLSTDAI
                     LKYFQNAYGNIPPDILNMFALAGIPEALNIIFGGFSFSFGIWSSYRVLKFLK"
     gene            complement(22051..23040)
                     /locus_tag="WJL87_000092"
     CDS             complement(22051..23040)
                     /locus_tag="WJL87_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798589.1"
                     /translation="MTVFDISDLNLKDPDQQDADDLGMTLADYKKMIQEDNDYGRIWQ
                     KIQLKKAEQLQKEKEEAVKRKRKRKIFSDYRGHRFKSVKELCYDVYKGLTYGRGWNGS
                     YQHIEVKGVDSGLYENNAELGRCNAVWPDGKVAHTLDYSVLYEDIPEGEKPEEEKKEP
                     EKDHPKPVEKPDSSNPKPSENPNVGTLGGGASPAVGNAPGGGSLGASDGGGSTGAGNG
                     GNNGSTAQGSGKGKGDGEDSEKMPDVPKYGEPDWGSLKSDGRFGSYATATAFSTGGHC
                     INDIRLDMGQFGSHTLQMGFLCDVLKKLRYVFIAMAYLYSAILVFKTVNSLKG"
     gene            complement(23027..23320)
                     /locus_tag="WJL87_000093"
     CDS             complement(23027..23320)
                     /locus_tag="WJL87_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798590.1"
                     /translation="MKFKRMAVAFVMSAFFVPAFSEDIEIHRVEEGFKKLNMTGKLYE
                     SVSNAPFGTVFPMRSRDGEIQYTMHKLGCFFNTCYFNVNKVPDNLKKRRHDGF"
     gene            complement(23389..23670)
                     /locus_tag="WJL87_000094"
     CDS             complement(23389..23670)
                     /locus_tag="WJL87_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798591.1"
                     /translation="MQCFSKKEQAEDYSVSKIVPVISPDGKLYAPYKSGGNWYFEGYR
                     IDLSFPQCSQESQFAQGAMLASSFLILFVIVWAFKTVGNMIKGSFANDG"
     gene            complement(23701..23928)
                     /locus_tag="WJL87_000095"
     CDS             complement(23701..23928)
                     /locus_tag="WJL87_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798592.1"
                     /translation="MFKSIKTNVRKAGAVLALATLSAPTWADGIEGVANGIVTEINKV
                     VPVVSSVGVALLSVYVLVKAFRLVSGFVAGR"
     gene            complement(24100..24402)
                     /locus_tag="WJL87_000096"
     CDS             complement(24100..24402)
                     /locus_tag="WJL87_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798593.1"
                     /translation="MKMIARVTGMKRSKGITEKGQSYDSTKLFIEIQFPESQDMCGYA
                     SQEFAYGTYENYEKLLASGVKMPFKAEIDFDFVTNGKAVKQIVTNVVPVFEKPKNS"
     gene            complement(24406..24690)
                     /locus_tag="WJL87_000097"
     CDS             complement(24406..24690)
                     /locus_tag="WJL87_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798594.1"
                     /translation="MIRVREKQYPCLVLDDYFQSGLITRRMYFSLDKFHHIPVSGAVI
                     YKDDPIYFRRLEKVLKANAKELDCYAYRVLADGRLFLVDRLITDFFDERK"
     gene            complement(24683..25786)
                     /locus_tag="WJL87_000098"
     CDS             complement(24683..25786)
                     /locus_tag="WJL87_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ7798595.1"
                     /translation="MDAKGNLKVVPLRRGKGDSAFIDTISFTFHESSVAKFFLITREF
                     FPIKGVSDRDVLVRFSDILEWIFGFGISSHQPVGKGRFYESRWQMERDGVLYGQVYIG
                     GQQETILVEMTGKGCAVAEEGWEERLYRFLTADTTYNAKITRCDVAKDFYENEISPDT
                     AWEMYEQGKFDKRGKRPLVGKLGDDWLNPTEKGKTLTIGSKHSSVFCRIYDKAKEQGD
                     TSGVFWCRFEQQYMGRNCYLSLDILLSPGSFWGGAFEICASLQQKGVTEHRCVSAKKR
                     LELSIERSKQVASQQVGRAINMMVFMGMSDYEIVDYLRRKDGAMPHRLNPASFMIKEK
                     RACEYIHDYEKEIEIIKFDEYGMVLDSLGDLND"
     gene            26312..26500
                     /locus_tag="WJL87_000099"
     CDS             26312..26500
                     /locus_tag="WJL87_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798596.1"
                     /translation="MKALRIKEEQEESIRRLAINANKKLIQLGREPLRDSELAHILLN
                     EAIRCAKIDDDGKITIGN"
     gene            26502..26819
                     /locus_tag="WJL87_000100"
     CDS             26502..26819
                     /locus_tag="WJL87_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798597.1"
                     /translation="MKKALKWIAVFIAFSVWSFGIFLYGFNNGTEAGMQCAQIKGNGL
                     CAMSFDGSASKIEFSDLNEKNDKQTLCFQSLWEFNDECEKKESNSIYYIDLPENNPKD
                     IKQ"
     gene            26901..27230
                     /locus_tag="WJL87_000101"
     CDS             26901..27230
                     /locus_tag="WJL87_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798598.1"
                     /translation="MLSQSQWLDAVKGRLRVRSDYALSKRWAVTPSEVSQYRRNRLRF
                     PFAVVLDIADVMDIDPIEIIAGLEYRRCREKDMERVKGAYFKAHCAHFVYPNNPGFFR
                     RKKRFFG"
     gene            complement(27299..27415)
                     /locus_tag="WJL87_000102"
     CDS             complement(27299..27415)
                     /locus_tag="WJL87_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HEZ7798599.1"
                     /translation="MTHLLERHRNARFMAHMDNFLPNWQSIKQQLNALELGV"
     gene            27368..>27652
                     /locus_tag="WJL87_000103"
     CDS             27368..>27652
                     /locus_tag="WJL87_000103"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798600.1"
                     /translation="MRHKTGVAMAFEQMGQFVDDDVLSGADTRTAPLGGNTLMCRFLI
                     AFLRISTFRLPIFILSSVFRVPDNQLKHCINIQPFFINEQLKLDFKAFLLH"
BASE COUNT         7042 a         7062 c         6856 g         6692 t
ORIGIN      
        1 tggctgcatt ggacgtttta atgtttttct gttttgtctt gcgccgctcg aaacgttttt
       61 tgcaatgggg cgatttgtgc cagccgcgcc cggtcgattt gttcggcgat aaggtgcgct
      121 tttccctgcg cgcggcattc ggcggcgatt ttgcccgaat ccgcctgatt ggcggcttcg
      181 agcagggcga accagtgtgc gcgttgcggg tagggcgtgt gttcgcggtt cagacggcct
      241 tgcgtgtcgg caatgcagac gttcaatgcc gtctgaaagc gttcgggacg gcggaaggca
      301 tcggtttttt tcaatacgtt cagaatggtt tggcttttaa gctgtccgac ttggtggaaa
      361 ataatgtgcc aacggcaaac caattcggca agctcggcgc aatgtttcgg cgcacgcagc
      421 cgctgattga cttcgcgcac gggttcgaca ccggcgaggt cgtgtccgtg gtggcgcggc
      481 aggatgtcgg acggtgtttt ggctttgccc aagtcgtgca gcagggcggc atagcgttcg
      541 ggcaggctca aacccatatc ggcggcgcgt tgcagcgtca tcagggtatg gatgccgctg
      601 tcgatttcgg gatggtagtc ggcgcgttgc ggcacgccga agagggcatt gacttcgggc
      661 agcaagactt tgagcgcgcc gcattcgcgc aacacttcaa tcattttgcg cggatttttt
      721 tccatcaaac ctttcgcaaa ctcctgccag acgcgttcgg caaccaatgc gtccgcttcg
      781 ccgttttcca ccatctgccg catcagcttt atggtttctt cggcgatttc aaacctgtaa
      841 cgcgcggcaa agcgggcagt acgcaggatg cggacggggt cttcggcgaa ggccggggaa
      901 acgtggcgca aaatgcctgc cgccaaatcc cgttgtccgc cgaaagggtc gataatcttg
      961 ccgtccgcat cttgcgccat cgcgttgatg gtcaggtcgc ggcgcatcaa gtcctgctcc
     1021 agcgtaacgt ctttgtcggc gtggaaactg aaaccgacgt aacctttggc ggttttgcgc
     1081 tcggtgcggg cgagggcgta ttcttcgtgc gtttcgggat ggagaaacac gggaaaatct
     1141 ttgccgaccg gctggaagcc ttgcgccagc atggtttgtg cgtctgcgcc gacgaccacc
     1201 caatcgcggt ctttgacggg caagcccaaa agataatcgc ggacggcgcc gccgacgagg
     1261 taagtctgca tatgcttcct attttaaagt tatcaacaat gccgtctgaa gcggcttcag
     1321 acggcattgt tccaaccggc ggttatgcta cgcctttttt ctccaagtaa ctttcgtaat
     1381 cgcccaagta gtgttcatat ccgcctttgc cgtccagttc gatgatttgg gttgccaggg
     1441 aagaaacgaa ctgacggtcg tgggagacga aaatcagcgt gccgttgtat ttttccagcg
     1501 ccatgttcaa ggattcgatg ctttccatat ccatgtggtt ggtcggttcg tccatgacta
     1561 agacattggg tttcaacagc aacagtttgc cgtaaagcat acggcctttt tcgccgccgg
     1621 agagaacctt cacttttttc acgacatcgt tactgccgaa gagcaaacgc cccaaagtgc
     1681 cgcggatgac ttgttcgtcg tcgccgtcct gcccccattg gcgcatccat tcgctcaaat
     1741 ccatatcgac atcgaagtcg ttttcgtgat cttgcggata gtagccgacg ctggcttttt
     1801 ccgcccattt gatgctgcct tcgtccggca gcagaccgtc tgaatattcg gggttgtacg
     1861 cgccggccaa gagtttcagc agggtggatt tgcccgcgcc gttcgggccg atgatggcga
     1921 ggcgttgtcc cgcttcaagg atgaagttca ggtttttaaa caactgggtt tcaaagcgtt
     1981 tcgccagttt ttcaacttcc acagcctgac ggtgcagctt ggctttttca tcggcttcaa
     2041 aacggatata cgggttttga cgggtagagg gtttgacttc gaccatttcc gatttgattt
     2101 tgtctgcctg cttcagacga ctggttgcct gacgggcttt ggatttgttg gcactgaacc
     2161 gcgccacaaa ttcttgcaat tcctgcaatt tttctttcgc cttggcattg tctttcaggg
     2221 cgcgctcacg cgattgggca gaagcgagca tgtagtcgtc gtagttgccc ggatagatgg
     2281 tgatggtgtt gtagtccaaa tccgccatgt gtgtgcagac ttcgttcaaa aagtggcggt
     2341 cgtggctgat gataatcatc gtggagtcgt attggttcaa cacgccttcc aaccagcgga
     2401 tggtattaat gtccaagtta ttggtcggtt cgtccaagag caatacatcc ggcttggaga
     2461 acagcgcctg cgccagcaat acgcgcagtt tgaagcccgg ggcgacttcc gccattttcg
     2521 cattgtgcaa atcttcggaa atgcccacgc cgctcaacag ttcggcggca cgcgcttcgg
     2581 cggtgtagcc gtcgtattcg gcgaacttgg cttccagttc ggcggctttc atgtagtcgt
     2641 cttcggtggc ttcgggattg gcgtaaatcg catcacgttc ggtcatcgcc gcccacattt
     2701 cggtatgccc catcatcacc acgtccagca cgcgcatgtc ttcgtaggca aactggtctt
     2761 ggcgcaattt acccaaacgc acgccgtttt caatcgccac ttcgccggcc gtctgttcca
     2821 aatcgccgcc gaggattttc atgaaggtgg atttgcctga accgttcgcg ccgatcaggc
     2881 cgtaacggtt gccttctccg aattttacgg atacgttttc aaacagcggc ttcgcgccga
     2941 actgcatggt gatgccgttg gtagaaatca tgattggtaa agcctttatt gaacaagaga
     3001 ttaaattttt cgaattttcg gattgtaaca taaccgcccc gccgtctgaa acagacgggc
     3061 aaattttcag acacttatag taaaatatcc gttttattat ttcagacaaa aggtttccaa
     3121 atgataatcc ttcacggcat tccaaattgc gacacagtca aaaaagccaa aaacaggctt
     3181 gccggatacg gcttggagtt tgaatttcgg gattttaaaa aacagaggcc gtctgaagcg
     3241 gaaatctgct cgtggctgga acaagtgcct ttggcaaccc tgctcaacaa acgcgggacg
     3301 agctggcgca aacttgatgc cggaacacag caaaaagtgc tgtcctcgac ggcggaggcc
     3361 gtcaaactga tgtccgaaat gccgagcctg atcaagcgtc ccgtattgga gtgcggcggc
     3421 agggtttatg tcggcttcag cgaagaaacc tacgacggca tcttcaatcg gcaagccctg
     3481 tgcagacagg gataaaaacc gttacaatac ccgacttgaa tttcccaatc ccatttccaa
     3541 tctgaaaata tgttccattc catcgaaaaa tacagaacgc ccgcccaagt ccttttgggg
     3601 ctgattgcat taaccttcgt cggcttcggg gtcagcacgg tatcccatcc gggtgccgac
     3661 tacatcgtcc aagtgggcga cgaaaaaatc agcgaccact ccatcaacaa cgctatgcaa
     3721 aatgaacagg cggacggcgg cagcccttcg cgcgacgcgg tgttccaatc cctgctgcaa
     3781 cgcgcctacc tgaaacaggg cgcgaagctg atgggcattt cggtttcttc cgaacaaatc
     3841 aagcagatta tcgtggacga tcccaatttc cacgacgcaa acggcaaatt cgaccacgcg
     3901 cttttaaacc gctacctttc ccaacgtcat atgtctgaag accagtttgt cgaagaaatc
     3961 cgcgatcagt ttgccttgca gaatttggta aacctcgtcc aaaacggcgt attggtcggc
     4021 gacgcgcagg cggaacagct gatcaggctg acgcaggtca accgcaccat ccgttcgcac
     4081 actttcaacc ccgacgaatt catcgcccaa gtcaaagtgt ctgaagccga tttgcagaag
     4141 ttttataacg caaacaaaaa agactacctg cttcccaaag cggtcaaatt ggaatatgtc
     4201 gccttgaatc tgaaagactt tgcagacaaa cagaccgtca gcgaaacaga agtgaaaaat
     4261 gcgtttgaag agcgcgtggc gcgtttgccg gcaaatgaag ccaaaccttc tttcgagcag
     4321 gaaaaagccg ccgtagaaaa cgaattgaaa atgaaaaagg cggttgccga cttcaataag
     4381 gcaaaagaaa agctgggcga tgacgcgttc aaccatcctt cctcgcttgc cgaagccgcc
     4441 aaaaacagcg gtttgaaagt cgaaacccaa gaaacttggc tgagcaggca ggatgcgcaa
     4501 atgtccggta tgcccgaaaa cctgatcaat gccgttttca gcgacgacgt attgaagaaa
     4561 aaacacaatt ccgaagtgct gaccatcaac agcgaaaccg cgtgggtcgt ccgcgccaaa
     4621 gaagtccgcg aagagaaaac cctgccgttt gccgaagcca aagacgcggt acgtcaggct
     4681 tatatccgta ccgaagccgc caaacttgcc gaaaacaagg caaaagacgt gcttacccaa
     4741 ctgaacggcg gcaaggctgt tgacgtgaaa tggtcggaag tgtccgtttt gggcgcacag
     4801 caggcaaggc agtccatgcc gcccgaggct tatgcggaac tgctgaaagc aaaaccggca
     4861 aacggcaaac ccgcctacgt caggctgatc ggtctgccgg cacccgtgat tgtcgaagta
     4921 caggctgtaa ccccgccgga tgatatcgcc gcacagcttc cgcttgcaaa acaggctttg
     4981 gcgcaacagc agtctgccaa tactttcgac ttgttgatac gttatttcaa cggcaaaatc
     5041 aaacagacca aaggagcgca atcggtcgac aacggcgacg gtcagtaatt gacacttttg
     5101 ttgacaaaat aacggtcgga atattccggc cgttttccca tacggcggaa tatgatgagc
     5161 cacaaaaaac aagatgcctt ccaaggattg atcgacgcgc tgaaggtttt acccaacgtc
     5221 gggccgaaat cggcacagcg gatagcgtat catttgctcc aacacaagcg caaagaggct
     5281 gaaaaactgg tggatgcctt gcagacggca ttgaagcagg tttaccattg cgcgatgtgc
     5341 aacacgtttt gcgaaggcgg attgtgcgat atttgtgccg atgaaacacg cgacgggcgg
     5401 cggctgatgg tggtgcatat gcctgccgac gtgtcgaata tggaagcggc aaactgccac
     5461 gacgggctgt atttcgtcct gatggggcaa atcaatacgg cattgggaat ggacgtatcc
     5521 gccatcgcat tggacaggct ggcgcaacgg ctgggcggcg gggaagtcga agaaatcatt
     5581 attgcaaccg cttttaccgc agaaggcaat gcgacggcgt atgtcctgtc cgagtttttt
     5641 aaaaacctgc cttacaaagt cagcaggctg tcgcagggca ttcccttggg cggcgaattg
     5701 gaatatgtcg atgcgggaac gctggcgcag gcggtgtacg aacgccgcct gattaaagaa
     5761 ggcggggcat aacgccgcct gattaaagaa ggcggggcat aacgccgcca atgcaaaatg
     5821 ccgtctgaag ccttcagacg gcatttttcc gggcggttta cttgcagttt agcccttgaa
     5881 tctgcaatgc gcctccgttg ccgactttga acacgcactg cgtcgcgctg ctgccggaca
     5941 cggtaatgct gtcgctgccg ccgttcaggc tgataggttc cttaacaccc ataccgactg
     6001 ccgttgccgc cagcttttgc gcgtcggctt cggaataagg cgtgttgttg gcatcgattt
     6061 tgatttcttt ggcgtaggca gccgcagcgg cggataaggc gatggcgcac aaagcaaaag
     6121 cagttcggat attcattgtt ttcctcaaga aacggttttt ctgaaggaaa ggatagggca
     6181 gtttgacgcg ttgtgcaagt tttccgcccg ctctgccgcc ccgtcccccg cactcgggcg
     6241 ttcggacgct gcctgggttt tacggtacaa taaacttttg ccgccgcccc caaaccgccg
     6301 tccggggcgg cacgtttgca gacattttta aggtagcgtt atgttttctc tagaggcttg
     6361 gataggcttg aggtatctca gggcgaaaaa gcgcaacggc tttatgtcgt ttatcacgat
     6421 ggtttcgatt gccggaatcg ccttgggcgt aaccgcgctg attgtcgtct tgtcggttat
     6481 gaacggcttt cagaaagaaa tacgcgggca gctcctgaat gtcgcgccgc acgccgaaat
     6541 cggctatatc gacaatacgg atacggattg gcgcaacctg cttcggttta ccgaaaaccg
     6601 caaaggtatt ttggctgccg cgccctatgt ttccaatcag gctctgctgg caaacgcggg
     6661 cgaaatcagg ggcgtgcaga tgcgcggcat tttgccgtct gaagagcgca aagtggtgga
     6721 atacggcgac aaaatgccgg caggcaaatt tgaagatctg attccgggcg agtttgacat
     6781 tatcctcggt gtcggcttgg cggaggcttt gggggcggaa gtcggcaata aagttaccgt
     6841 catcacgccg gagggcaacg ttacccctgc cggagtcgta ccgaggttga aacagtttac
     6901 cgtggtcggt ctggttaaaa cgggtgttta cgaggttgac aattcattgg cgatgacgca
     6961 tatccaagac gcgcgcgtac tgtaccgctt ggataaggaa gttgcggggc tgcggctgaa
     7021 actcgccgac ccgcaaaacg cccccgcctt gacggcaaca ctgattccgg aggcgcaaag
     7081 ggacgcggtt tgggtgcgcg attggacgta caacaaccgc agctattttg aggcggtcga
     7141 gttggaaaaa cggatgatgt tcatcatcct gacgctgatt atcgccgtgg cggcgttcaa
     7201 ccttgtctct tccttggtga tggcggttac ggaaaagcag gcggacattg cgattttgcg
     7261 gactttgggt ctttcccctg gcggcgtgat gaagattttt atggtgcagg gcgcgttttc
     7321 aggctttttc ggcacgctgg cgggtgtggt ctgcggcgtg cttttgggtt ggaacgtcgg
     7381 cagggtcgtg gcgttttttg aaaacctgct cggtgtccac cttatcaatt cgcaggttta
     7441 ttttatcgac tacctgccca gcgatgtcga tatgggcgac gttgccctga ttgcctgcat
     7501 ttccttggga ctgtctttcg ttgccacgct ctacccgagc tggcgtgcct caaaaaccca
     7561 accggcggag gctttgcgtt atgagtgagt tgattttgaa atgcgaaggc gtgggcaaac
     7621 gctaccgcga cggcggtttg gacgttcggg tgctgcacgg cttggatttg gaaatccgcg
     7681 cgggggaaag caccggcatc atcggttctt cgggcagcgg aaaatcgacg ctgctgcata
     7741 ttctgggcgg gctggatatg ccgtctgaag gcagggtgct gctgatgggc gaggatttgc
     7801 gtacattaag ccagcgacaa ctgggcatcc tgcgcaaccg ccatctcggt ttcgtgtacc
     7861 agttccacca tcttctgcct gaattttcgg cactggaaaa cgtgatgatg ccgcttctga
     7921 tcggcaagaa aaaacgggaa gaggcggcag aaacggcaat ggcgatgctg gaaaaggtcg
     7981 gactgaaaca ccgttcgacg caccgcgcgg gcgaactgtc cggcggcgaa cgccagcgtg
     8041 ccgccattgc gcgcgccttg gttacgcaac caaaatgcct gcttgccgac gaaccgaccg
     8101 gcaacctcga ccgtgcgaac gccaggaatg ttttggatat gatgctggaa ctgaaaacgg
     8161 aattgggaac ggggctggtg gtcgtaacgc acgacgacga actcgccggc cgcttcgagc
     8221 gcgtgatggt catgaaagac ggcagcctgc accccaaaca gggcgcaaac gcctaaacag
     8281 ccgaaacaac accgcgccgt ctgaagcccc acgccttcag acggcatttc gacaaggaat
     8341 acaatggaac ggcagacaga tgaatttgcc caagcggcgg caagggcggc aatctggttt
     8401 ttggcacgct atgcgggttc aggcagcgaa gtgcttgcaa actgcaccga acgcctgttt
     8461 caggcattgc aaaacggtca ttcgtttatc cgtttgagcg gtggcgaggc tgacgcgctg
     8521 tcggcactcg cacccgtggt cggaacatcc gccgcgcctt tgattttgga aggcagaagg
     8581 ctgtttttgg gcaggatgtg gcagttggaa tacgatttgg ctgccgagat aaaacgcttg
     8641 gcggcagccg acacgtccgc ccccgacgcg gcaggcgcgc ggcaaaacct cgcaaaatgg
     8701 tttcaaggca cgggcagcga agggcagcgc gatgccgccg ccttggcact gttgcagcct
     8761 tttatggtga ttaccggcgg acccgggacg ggtaaaacga cgacggttgc caaactgctg
     8821 gcgctgattt gcggtgaaga cgaaaatctt cccaacatcg cgcttgccgc accgacgggc
     8881 aaagcggcgg cacatatggc gcgcgcgctg caccgtgcaa tcaacggttt tgacgcgccg
     8941 gaggccgtcc gccgccattt gctcaaactg gaagggcaaa ccgtccaccg actgctgaag
     9001 ctgcgcccgc ccaaaatgca ggcggcgttc gaccctgttt acccgctgcc gtttgacgta
     9061 ttggttatcg atgaagcctc tatgctggat acggcattga tgctgcaact tttaaaagcg
     9121 gtcaaaaccg gcgcgcgcgt gattctgctg ggggatgaaa accagctccc gtccgtcgga
     9181 atcggggcgg tgctgtccgt tttgtcgcaa aaaaccgttt tggacggaga agcgcaccaa
     9241 aggctggccg gcttccttcc ggaacacggt ttcagcgtca gcgcaaatcc gcccgtgttg
     9301 gcgcaaaaca ccgcccatct gtcgttcagc caccgtttcg gcgacaacag cggcatcggc
     9361 tgccttgccc gtgccgccgt atcgggcgat gagggggcgt gggcattgtt tgaccggttt
     9421 ccggatgaac tggaacattc ggaatgcagt ccgaacgctc gagtcgaaag gttgtaccgg
     9481 gcgcacaaag cctattggca ggcggtaaaa gacggcaata tcgaagccgc atacgcaggc
     9541 atctcggata tcgtggtcct ggcagcttgg cggcaggatg cggaagactt caacaaagcc
     9601 tattgcagcc atgtacgccg caaaatgaac ataccggaac atctggccta tttcgccgga
     9661 gagccgatta tgatcaggca gaacgactac gcgcttgaac tgttcaacgg cgacatcgga
     9721 ctgattatgg aagatgtcgg acggcagggc agccttgccg cctattttgc cgatgcggac
     9781 ggatttaaaa aggtagcggt aagctgcctg cccgatttcg aacccgcatt cgccatgacc
     9841 gtccacaaaa gccaaggttc ggaatatcgg gaagtctggc tgctgccgcc ttccgccgca
     9901 ccttcggacg aaggggacga tgcattgtcc ggattgagta aggagctgtt atataccgcc
     9961 attacccgcg cgagggagaa gttcgtattc ttcggcggag aagaaacctt ccggcaagct
    10021 gccgccactg tcaaaacgcg tcagacggca ttgggcagta tgctcgagcg ggtattttca
    10081 caagaataat ccgcccgaat gccgcgccgc cgccccttat gcctttttca aacggtatag
    10141 gaaagtggtt tcccgggttc gcgcaaaagc aagcggatcg ctcggattcg cggctttttt
    10201 gtgcttcggc ttggttttca tcatatcggc aacacgcaaa cccgcccgga taaatgcctt
    10261 ttccaaaaaa tcggcagggc gcagccccaa atgctctgca aggtcggaaa taatcaggcg
    10321 gatttctccg tcgggattca gatgtttcgg cgcatcccgc aaaaacgcag ccagcatcgc
    10381 agattcgggg tcgtataacg cggattcgac ggcggaagtc ggcttggcgg gaagccaggg
    10441 tggattgcag acaatcagat cggcaaaccc ttcgggaaac agatcggttt cccgtatctc
    10501 aacctgtttt tcaaagccca aacgggcaat attggcacgg gcgcaggcga cggctttcgg
    10561 attggtatcc gtgccgatga cggaaggaat gccctgtttc gccaaaatgg cggcaagcac
    10621 gcccgagccc gtcccgatat cgaatgccgt ctgaaaaccc gttgacggcg catgggcgag
    10681 caggtcgagg tattcgccgc gcaacggcga gaatacgccg aaaggaacgt gtatgctgcc
    10741 gcccagctgc ggaacggcaa cccctttctt atgccactcg tgcgcaccca taaaccccag
    10801 cagcagattg agcggcagga aaaacggttt gccgtccgcc tctccgtaca cgtcgagcag
    10861 ggcggcgcgt atatcgggcg cgcgtttgtt gtccaacaca aaaccggggc ggatttcaac
    10921 ggcaagcata ttcagaatac ggctctgctg cgcctgcttc atacggtggg catggaaaag
    10981 ggcggcgata tccgcatcgg aacggacggc ggcaggtttg cgagccctct tcttcattgc
    11041 agaaagcacc tgtttggcat tgtggaaatc gccctgccag acagttgcaa tattttgata
    11101 ggcagccttc aaaatgccgt ctgcaccgct ttcgcggaca taatgccaac ctttgggcgg
    11161 cttttgcaca ctttcgttgc gccattcggc cccgttatcg ggaaaaataa aagaagacat
    11221 gggatacctg cgtcatgttt tgaaaatagg gcggcagaac cgtaaaccat acggatggta
    11281 cagcaaggag cggcaacaca gaacagtttt ttgttcccgc cttgtctttc caagcccatg
    11341 cagtctgaag ccggaatgtt tcagacggca tcgcatcaaa ctccataaat aaaccacata
    11401 tgcttgaaat aataccttca accccaatgt acgcgaaaat cggcaatctg tcagacacaa
    11461 gagagtacct atgacacaaa aagaaaagca ttttgaggaa tatgccgcct tggcaaccct
    11521 tcctttgcgg gatgtcgtcg tttacccgca tatggttctg ccgctgtttg tcggcaggcc
    11581 gaaatccatc gccgcactgg aaaacgccat tacccgcgag gaaccggttt tcctattggc
    11641 acaaaccgat gcggcgctag aagaaccggt tgccgccgac ctgtatcaga ccggtaccgt
    11701 cgcacaagtc ttacaggtat tgaaactgcc ggacggcacg gtaaaagtat tggtcgaagg
    11761 tctgtatcgc ggacgtgttc tgaccatcga agacacgggc ggtctgttcg tttcccatat
    11821 agaggcggtc gtggaagaag acacgggcgg caataccgac ctcgaagccg tgcgccgcac
    11881 cctgttggcg cagtttgaac aatacgccaa actcaataaa aaaatccccg ccgaaattat
    11941 cggcagcatc aacggcattg ccgaaaacag ccggctaacc gatacggtcg cagcgcattt
    12001 gcagttgaaa ctggcgcaac gccaacagat tttggaaatt cccgaaatcg gcaaacggat
    12061 ggaattcctg ctggcacagc tggaatccga actcgacatt atgcaggccg aaaaacgcat
    12121 acgcggacgc gtcaaacgcc aaatggaaaa atcccagcgc gaatattatc tgaacgaaca
    12181 gattaaagcg atacacaaag aattgggcga agaagacgaa aacggcgaac tggatgcctt
    12241 ggaagcaggc atcaaaaagg cggggatgag caaagaagcg gaagaaaaat gcctgtccga
    12301 actgaaaaaa ctcaaaatga tgccgccgat gtctgccgaa tccaccgtcg tacgcaacta
    12361 catcgacacc ctgctcgaac tgccgtggaa gaaaaaatcc cgcgtcagca aagacatcgc
    12421 caaagccgga ttggtgctgg ataccgacca ctacggcctg gaaaaagtca aagaacggat
    12481 tttggaatac ctcgccgtcc aaaaacgtat ggacaaactc aaaggcccga ttctgtgcct
    12541 ggtcggccct ccaggcgtgg gcaaaacctc tttgggcgaa tccatcgcca aagcaacggg
    12601 gcggaaatat gtccgcatgg ctttgggcgg cgtgcgcgac gaaagcgaaa tccggggaca
    12661 ccgccgcacc tatatcggct ccatgcccgg taagattttg cagaatatgg caaaagccgg
    12721 cgtaaaaaac ccattgttcc tgctcgacga aatcgacaaa ttgggtagcg acttccgggg
    12781 cgatcccgcc agcgcgttgc tcgaagtgct tgatcccgag caaaacaaca agttcgccga
    12841 tcattatgcc gaagtggatt acgatttgag cgatgtgatg tttattgcga cttcaaacag
    12901 cctgaatatc ccgacaccgc tgctcgaccg tatggaaatc atccgtctgt ccggctatac
    12961 cgaagacgaa aaaatcaata tcgcgatgca gtacctcgtg ccgaagcaaa tgaagcgcaa
    13021 cggcgtaaaa gaaggggaat tggcaatcga agaaagcgcg gtgcgcgata ttatccgtta
    13081 ctacacccgc gaggcgggcg tgcgttcgct cgaccgcgag attgccaaaa tctgccgcaa
    13141 ggtggtgatg cagattacct tggacgaaga ctcaaaaacc aaaaaaaccg gaaaagccaa
    13201 gcccagggcg gttaaagtaa atgagaaaaa tctgcacgac tatttgggtg tgcgccgctt
    13261 cgattacggc gttgccgaaa gcgaaaaccg tatcggacaa gtaaccggtt tggcgtggac
    13321 ggaagtcggc ggcgaattgc tgaccgtcga agccgcagca ttgccgggta agggcgtgat
    13381 tcagtgcacc ggccagttgg gcgatgtgat gaaggaatcc gtgtccgcag cgtggtcggt
    13441 tgtccgctcc cgtgcggaat cagtgggttt ggctcctgat ttttacgaga aaaaagacat
    13501 ccacatccac gttcccgaag gcgcgacgcc gaaagacggc cctagtgcgg gtattgcgat
    13561 gaccttggcg gcggtatctg cctttaccaa aatcccggta cgcgccgatg tggcgatgac
    13621 gggcgaaatt accctgcgcg gcgaagtcct gccgattggc ggtctgaagg aaaaactgtt
    13681 ggctgcgctt cgcggcggca tcaaacacgt tttaattcca aaagacaacc tcaaagactt
    13741 ggaagaaatc cctgaaaacg tgaaaaccgg cctgaccatc catccggtca aatggataga
    13801 cgaggtattg gctctgggtt tggaaagcca gcctgagcct tgggcagaac attctggtgc
    13861 ggaagcggcg gcggaatccg cttcaaaacc aaaaccccgc agcagggcaa ccaaacattg
    13921 aaacgcagga aatgtgttgt aaaaatgcgg tttcgtcctg aaagcctgtc aaatagggtg
    13981 attccgtatt ttttgcttga cacggcaatt tcagaattgc tataaagcga aagttgctca
    14041 agcagtacaa accccgagta ttcgggcatt tcatcatttt ttaagacagg aagggactga
    14101 ttgtgaacaa gtctgaattg atcgaagcga ttgctcaaga agccgacatt tccaaagccg
    14161 ccgcacaaaa agctttggat gccactacca atgcagtaac caccgccctg aaacaaggcg
    14221 acaccgttac tttggtcggt ttcggtactt tctacgtggg cgaacgtgcg gaacgccaag
    14281 gccgcaaccc caaaaccggc gagcctctga ccattgccgc cgccaaaacg cctaaattcc
    14341 gcgccggcaa agctttgaaa gacgcactgt aagccgtttt ttatgaaaaa agccgattct
    14401 ttaaagaatc ggctttttta tcggtccaca ttattctgat ttcaaatcgg caacacactg
    14461 cttgtcacgt gcttcaaagg catttgcgcc gccgagcagg tcaagctgtt cttgtgcgcc
    14521 gagtttgccg aaggatctaa tctgtttctc gctcaatctg tccaaaggct gctcccacat
    14581 acatttgcag tagtcgacgg taaggcgggt attgtttgaa tctaaaccgc gggcccgtaa
    14641 atcgttttgc catttttcgg caaacggaat attcttcacg caagactcga caattttctg
    14701 ttttgcctgc ggtttggaca tcgcgcattg ggagagcagg gcggttgaag cgagcaacgc
    14761 caaaatgacc catgcccaaa tgcggatggt gcggattttg gcttttgctt ttttgcgcgc
    14821 ggcaacctgc tcttccgtcg gcatttcgtg tttcggctca gtcatgcagg ctttccatgc
    14881 ggatcatggt aatcggtttt tccacgcaat ccagtgcttc gatggctgcg attgccgact
    14941 tgatgtgttt ttcgaccgtg ctgtgggtca gaatcacgat ttcggcagtg gtctgatcaa
    15001 tcacgccttt ttgaatcagt gcttcgatgg acacgttttc ttgtgccaac agcgcggcga
    15061 tttgccccag cgtgcccggt tcgtctttgg cttggacgcg caggtagtag ctgctggtaa
    15121 tttcgtccat aggcaggatg gtttgcgctt ggacttgcgc gggttggaac gccagatgcg
    15181 gtacgcggtg ggcggtatcg gcttcaacca ggcgggcgat gtcgatgata tcggcaacca
    15241 cggcggaagc ggtcggcaat gcgcccgcgc ccgcgccgta atataaggtt tcgccaacca
    15301 tatcggcgtt gacgcgcacg gcgttcatca cgccgttgac gtttgccaag aggcggcttt
    15361 cgggaatcag ggtagggtgg acgcgcagct cgatgccttt gccggttttg cgggtgatgc
    15421 ccaacagttt gatgcagtag ccaagttctt cggcgtattt gatgtcgcgg ctgtcgagtt
    15481 tgctgatgcc ttcgaggtag caggcggaaa agttcatcgg cgtgccgaat gccagcgcgc
    15541 tcatgatggt gattttatgg cccgcgtcat gtccttcgat gtcgaaggtc gggtcggctt
    15601 cggcatagcc cagtgcctgc gcttctttca gtacatcggc aaacgcgctg cctttttcac
    15661 gcatttcgga gaggatgaag ttgctggtgc cgttaataat gccggcgatg gatttaatcc
    15721 tgtttgccgc caaaccttcg cgcagggctt tgatgattgg gataccgccc gctactgccg
    15781 cttcaaattg caccatgaca tttttttctt ccgccagcgg gaagatttcg ttgccgtatt
    15841 cggcgagcag ttttttgttg gcggtaacga tgtgtttgcc gttttcaatg gctttcaaca
    15901 ccgcatcttt ggcaatgccc gtgccgccga acaattcgac gacgacatcg acgtctttac
    15961 gcgcgaccag ttcgaacgga tctttgacaa aggctgcgga cgggcagatt tggcgggctt
    16021 tttcttcgct caagtcgcat acggcactga tgcgcacttc gcgccccaag cggcgggaaa
    16081 tttcctccgc gttgtcccgc aacacggcag ccgtaccgcc gccgaccgta cccaaaccta
    16141 aaagaccgat gtttactggc ttcattgtgt ctccttgtaa gccgactgaa atgtaaatat
    16201 tgaaagacga aaatatccgc tgccgatata attgtgccgc actttgaatc aaatgccgtc
    16261 tgaaatcagc aggagaggtc agatgaaatc cgctaatcct acatgaattt gtctgatttt
    16321 gcacccctcc tgatacagat gaagcggcaa caggataaaa aaatgttatt tgaagaaaat
    16381 ccgatagacg aacattttgc agagtatgac cgccgcacgg gtgaaatccg gctgtccggc
    16441 agacatttca ataagcctgt gctgctgcat agggattctg ccggcctctc gaaatggcag
    16501 tcattgtcag aactgactcc ggataatctg ttgtctgatg tcaaagcgga tgactatccg
    16561 gaaatattga ttatcgggac gggcgcggct caggagttta tccatcccaa aatcatggcg
    16621 gatttttccc gaatcggaat cggcgtggaa tgtatgaata ccgattcggc attcaggaca
    16681 ttggttttcc tgcactcgga agggcgcagg gcttgggctt ggcttcagcc gtaaatttcc
    16741 cgtttcagac ggcatcggca ctgactttca ggtaaaatac gggcttttcc cgcccgacga
    16801 tgtttccgtt atgattgaaa tcaaaaacct caccctgcaa cgcggtttga aagtcctgct
    16861 cgacaaagcc aacgctaccg tcaatcccgg tcagcgcgtc ggtttgatcg gcaaaaacgg
    16921 aacgggcaaa tcgagcctgt ttgccttaat caagggtaaa atcactcagg acggcggcga
    16981 tgtctcgatt ccgaaaaact ggcggctcgc ttccgtttcc caagaaacgc ccgatttgga
    17041 tatttccgct ttggattacg ttttgcaggg cgatgccgag ttgcaggctt ttcagacggc
    17101 attgaggcag gcggaagcgc aaaatgacgg catgaagcag gcggaatatc atgccaaatt
    17161 ggaagaaatc gacgcttata ccgcgccggc ccgcgcggca aaattgttga acgggctggg
    17221 tttttcgcaa gaagaacaca gccgccccgt caaatccttt tccggcggct ggcgtatgcg
    17281 cttgaatctt gcgcaagccc tgatttgccg cgccgatttg ctcttgcttg acgaaccgac
    17341 caaccacttg gatttagaaa ccgtcttgtg gctggaaaac caccttgcct ctttaccctg
    17401 cacgcaaatc atcatttccc acgaccgcga ttttctcaac gcggcaacta cccaaaccat
    17461 tgaattatcg cagcaaaaac tcacgcaata cggcggcaat tacgattttt accaaaacga
    17521 acgcgcgcag cgtctcgcgc aacaacaagc tgcctatgtc aaacagcagg cgcaaatcaa
    17581 acatctgcaa tcctttatcg accgcttcaa agccaaagcc accaaagccg ttcaagcgca
    17641 aagccgcatg aaggctttgg caaagctaga acgcgttgcg cccgcgcatc tggacagcga
    17701 gttttccttt gagttttacc atcccgacca tctgcccaat cctttgttaa agctagaaca
    17761 cgctgatttg ggttacgaag gcaaaaccgt tctgcatgac attaccctgt cgctggaaag
    17821 cggcgcacgc tacgggctat tgggtgtcaa cggcagcggt aaatctacct ttatcaaagc
    17881 cttggcgggc aaaatcgatt tgctttccgg cagcatcgtc cgttccgaaa aactcaatat
    17941 cggctatttt gcccaacacc aactcgacac catccgcgcc gaccaaaacc ctgtttggca
    18001 tattcagcag ctttctccca aagtacgcga acaagaaatc cgaaatttcc tcggcggctt
    18061 caattttgtt ggcgatatgg cgttacagaa aaccgaacct ttttccggcg gagaaaaagc
    18121 ccgccttgcc cttgccatga ttatctggca aaagccgaac ctgttgctgc ttgacgaacc
    18181 aaccaaccat ctggacttgg atatgcgcca cgccctgacg ctcgctctgc aaagtttcca
    18241 aggcgcctta atcatcgtat cgcacgaccg cagcctgctt gaagcgacta ccgacagctt
    18301 cctcctgatc gacaaaggcc gtctgaagaa cttcgacggc gatttgaacg attaccgcca
    18361 atggcgtttg gcacaggaaa acgccgccgc cgcacccgca gcatccgcac aaagccaaag
    18421 ccgcaaagac accaagcgca tcgaagcgca aatccgtcag gaaaaagccc gacgcggcaa
    18481 gccgatacag cagaaaatag accgtgccga aaaagaaatg gcgcagcttt ccgaaattca
    18541 gacggcatgt gaagcatttt tagcacaaga agacgcttac cttgaagcaa acaaagaaaa
    18601 attgcaaaat accttatccc agctggcaaa agtcaaaaca caacttgccc aaatcgaaga
    18661 ggtttggctg gcttgtcaag aagaattgga acggattgaa actgaaatcg agaaacagtt
    18721 tgccgagcga taaagaagcg cgggcagggt gcttatcttt gcctgccaat aacggtataa
    18781 ttcgggcgtt atcaccgcct tttaccggta taaacatcag actttttgcc gccttgggtc
    18841 tcctgtccct ttccggcgcg gcggcgcaag cctccgtata ccattgcaac tcaaatggca
    18901 aaagcgtata tacatcagcc ccagcaggat cgtgcgccga tgcggattta cctaaaatca
    18961 gcagccatca gggaggcgga taccgcctga aaattaaaaa actcagtcaa gaagccaaaa
    19021 tacacataga aagtaaaaag aaaaacaaaa aacctgccgg gaaaaagaac aggcaggctg
    19081 ccaaagcccc gaaagaaaat caaaagcaac caaccgaaaa gaaaagcccg taaaacgcca
    19141 agaaagcatt ataaaaaata ccaaaaaatc aaatgattat ccgaaaatca agcacattat
    19201 gaaatcaaaa ctccccttaa tcctaatcaa cttttccctg atttcaagcc cattgggtgc
    19261 gaatgcggcc aaaatctaca cctgcacaat caacggagaa accgtttaca ccaccaagcc
    19321 gtccaaaagc tgccactcaa ccgatttgcc cccaatcggc aactacagca gcgaacgcta
    19381 tatcccgccc caaacgcccg aaccggtatc atcaccgtca aacggcggac aggttgtcaa
    19441 atataaagcc ccggtcaaaa cagtatccaa gccggcaaaa tccaatacgc cgccgccgca
    19501 acaagcaccc tcaaacaaca gcagacgctc cattctcgaa acagaattga gcaacgaacg
    19561 caaagcattg gttgaagccc aaaaaatgtt atcacaagca cgtctggcaa agggcggcaa
    19621 catcaaccat caagaaataa atgcattaca aagcaatgta ttggacaggc agcaaaatat
    19681 tcaagccctg caaagggaac tggggcgtat gtaaagccgt gttttcaaat cgaccgttcc
    19741 aaggatttga cagaagaaat gatgaaaaag cagcggcata tcctgtgtgt cggctgcctg
    19801 tatattatat tgaaggtcaa cgtatttccc aataccggct ggatggaagc gtcgaaaggg
    19861 atgcagggca tgtcgttcag taccttcgga tacgcggatg ctccgggcta tacattcagc
    19921 cgcattggcc atactttaat tttcctactt ggaaagacca cagggacacg tacaactgtt
    19981 tccgatagtt cgcataatga caagttacgt ctttttctaa gaatattaca ggttttggca
    20041 aaggtttctg ttgcgacccg aatgtcttat ttttttaggc gtatctcagt ccggtatcac
    20101 tccgttagtg ggtttccggt attgaaaaac agttcagaaa aaaggaaaag ggggtattcg
    20161 taaagattgg gcaaaaagcg cgcccaatct ttacaaagct tccccctttt ccttttttct
    20221 gccctatttt cctgcaccta caacccccga acgaagcgat accagactga gacactttaa
    20281 aaaaaacagc cattctagca gttaaccccc ttcactccgc tcaagccatc ctgagagggc
    20341 gggggctgaa accgaaagat ttttattttg aatgaaagga actttatgac atagattcag
    20401 gcgcgagagg cgagaaacct aaaagagcgg tggggacaaa caaaaatccc caagctgaca
    20461 acaaggggat ttttgcattc ctgcctagtt tttaaacttt acaggataaa tcaaaatgca
    20521 gatcactttg cgaattggta aactgctttt gactataacg attgatatac ggcttattct
    20581 agccgttatc atactgataa gtcaataacc cacgactata accgcccgat taagggcggt
    20641 tttctatttt gccgcatcca tcactaatac ttgttttttt aaaggtttct gttcgtcggt
    20701 atctatctta atttctcttt gttcttcttt atacggattg aacggcattc cgttatcgac
    20761 gtaatcgata caggtttttt tgtctatctc ttttaaaatg gttgcctgcc ctgaatagca
    20821 gttgcatgaa tttgacgatt tgacacaggc aacggggaac tccatttgtc tgacctgcct
    20881 gaccccgtta taaatcggtt ttgattcggg atgaccgtct attgtgggtt ctaacatctc
    20941 tttcgtcaaa ttctgatttt ctttgtccga gcccaactga ttgcctatat ttcttccggc
    21001 ttcgcccgat gccttatacg ccatgcccct gacttgttct aattgttcct ccgaaacgat
    21061 tgtttgcgtt ttagcttctc cttcctctgt tccgtcctta ttacccagcc ccaataatag
    21121 cttgtagccc aaataaaaga atagggcgaa caataccaat gcgatgggga tgatatataa
    21181 aacgcggctt ttcggggttt ttaccttagt atggatttcc gctgatttat aaagcccgaa
    21241 cgccttttta tccaacttat agacttcggg gcgtgcattg gcgaactctg accttggatt
    21301 attcgcacaa tagtcccaaa aataacgcat aaaaatgccc agcggggttt tatggatatg
    21361 ataatgcgcc cctaccaaat cgcggacgtg cttatctatg cgttggggca tctgcgttat
    21421 caggataata tctatgcccg aatgcctgtg aacgtgcaac cattcgacaa gaggcggtgc
    21481 ttttacggca ggggagcgcg gcgggaaaac attttgcgct tcgtctatga tgacgacaga
    21541 gccgttattt tcgggatatt gaagccaaac gtgcatatca ttaatggaat gaccttcggg
    21601 aatgggcgtt gtttctatcg ttaattccgg aatgccttca ataaaaatct tcctgtcttt
    21661 ccactcatta tttatctttt tcgccaagtc cgaaacgacg gataaagttt tgcccgaacc
    21721 gggaacgcct gtaattaatg taatcattct ttgcctttca ttttaggaat tttaaaacgc
    21781 ggtaggaaga ccatatgccg aacgagaacg agaaaccgcc gaatatgatg tttaatgctt
    21841 cgggaatgcc tgccagtgca aacatattta aaatatcggg cggtatattc ccataggcgt
    21901 tttgaaaata tttcaaaatg gcatcggttg aaaggcttaa cgcctcatat gagacaaacg
    21961 acgcgccaag acctgcgaga attttaaaaa acaagtcttt aagcatcggc accaatgcca
    22021 tgaacaatgc gacaatcaat cttggcattt ttaacctttc aaactgttta cggttttaaa
    22081 aaccaagatt gcggaataga ggtaagccat ggctataaaa acatatctca gctttttcaa
    22141 tacatcgcac agaaagccca tttgaagcgt gtggctgccg aactgtccca tatccaacct
    22201 tatatcattt atgcaatgcc ctcccgttga gaacgcggtt gcagttgcat aacttccaaa
    22261 ccgtccgtca cttttaaggc ttccccaatc aggctcgcca tacttgggaa catcgggcat
    22321 tttttcactg tcttcgccgt cccctttgcc tttgccgctt ccttgtgccg ttgaaccgtt
    22381 attaccgccg ttccccgcgc ctgtgcttcc tccgccgtcg gatgctccga ggcttccgcc
    22441 acccggcgca tttccgacag cgggggaagc accgcccccc agtgtcccaa cgttcgggtt
    22501 ttcagacggc ttgggattgg acgaatcggg cttttctacg ggcttcggat ggtctttttc
    22561 gggttctttt ttttcttctt cgggcttttc cccttcgggt atatcttcat acaagacgga
    22621 ataatctaaa gtatgggcaa ctttgccatc tggccaaaca gcgttacatc tccccaattc
    22681 cgcattattc tcatacaaac cggaatcaac gccttttact tcgatatgct ggtaactgcc
    22741 gttccaccca cgtccataag tcaaaccctt gtaaacgtca taacagagtt ctttgacaga
    22801 cttgaatcta tgccccctat aatctgaaaa aatcttcctt ttccgcttcc tctttaccgc
    22861 ttcctctttt tctttttgta attgttctgc cttttttaat tggattttct gccaaatcct
    22921 gccgtaatcg ttatcctcct gaatcatctt cttataatcc gccagcgtca tacccaaatc
    22981 atcggcatcc tgttggtcag ggtcttttaa atttaaatct gatatgtcaa aaaccgtcat
    23041 gtcttctttt ttttaaatta tcaggtactt tattcacatt aaaataacat gtattaaaaa
    23101 aacatcctaa cttatgcatt gtgtattgta tttcgccgtc cctacttctc atcgggaaaa
    23161 cagtgccaaa aggagcattt gaaacagatt catacaattt cccagtcata tttaactttt
    23221 taaaaccttc ttcaacacga tgaatctcaa tatcttccga aaaagcagga acaaaaaaag
    23281 cggacatcac aaaagcgacc gccattcttt taaatttcat ctaatacccc ctatttgaaa
    23341 aaataaacca atgaaatcac tgcaaaaaat ccaataagaa aatagaaatc aaccatcatt
    23401 tgcaaaactt cctttaatca tgtttcctac cgttttaaat gcccatacga tcacgaataa
    23461 aatcaggaac gaagaggcaa gcattgcccc ttgtgcaaac tgtgattctt gggagcattg
    23521 cgggaaagac aaatctatcc gatagccttc aaaataccaa tttccgcctg atttatatgg
    23581 cgcgtacagt ttcccatcgg gagatataac gggcacgatt ttggatacag aataatcctc
    23641 tgcctgttct tttttggaaa aacactgcat tcctaccctg tatcccatca tcacaccttt
    23701 ttaacgacca gcaacaaagc cggagacgag gcggaatgct ttaaccagca cataaacgga
    23761 gagcagggcg acgccgacgc ttgaaacgac cggaacaact ttattgattt cggttacgat
    23821 gccgtttgca acgccttcga tgccgtccgc ccaagtcgga gcggaaaggg tagccagtgc
    23881 taaaaccgca cccgctttac gaacatttgt tttgatgctt ttaaacataa aatttccttt
    23941 ttaaaaatta cggttttcgg aggcaaaccg cacagcctta aaatggaaca tcggagtaga
    24001 gttcttcata atcttccgat tcatcgtcaa aatgaaatta ctgaattatc ggcgatccgc
    24061 ctgttgaagc agccggaagc cacgcactta cgtgatattt catgagtttt taggtttctc
    24121 aaaaacaggg acaacgttag ttacaatctg ttttactgct ttgccgttgg tgacgaaatc
    24181 aaaatcaatt tctgccttga acggcatttt tacgcctgaa gcaagcagtt tttcatagtt
    24241 ttcatatgtg ccgtatgcga actcttggct agcatatccg cacatatctt ggctttcagg
    24301 aaactgaatc tcaataaaga gcttcgtaga atcataagat tgaccctttt ccgtaatgcc
    24361 tttgcttcgt ttcataccgg ttacacgtgc aatcatcttc atttgctatt tcctttcgtc
    24421 aaaaaaatcg gttattaacc tatctactaa aaacaacctt ccatctgcta aaaccctata
    24481 cgcataacaa tcaagttctt tagcattagc ttttaaaact ttttccaagc gtctgaaata
    24541 tatagggtca tccttataaa taaccgctcc agatactggt atatgatgga atttgtccaa
    24601 agagaaatac atacgccgag taattaagcc gctttgaaaa taatcatcta aaaccaaaca
    24661 aggatattgc ttttctctta ccctaatcat ttaaatctcc caaactgtct aaaaccattc
    24721 cgtactcatc aaatttaata atttcaattt ctttttcata atcatggata tattcgcaag
    24781 cgcgtttttc tttaatcatg aatgatgcgg gatttaaacg atggggcatt gcaccatctt
    24841 tccggcgcag ataatcaacg atttcgtaat cagacatacc cataaaaacc atcatattta
    24901 tggcgcgccc gacctgttgc gatgcaacct gcttagagcg ttcaatgctc aattccaatc
    24961 tttttttggc acttacacac ctatgctccg tcacgccttt ttgttgcaat gaagcgcaaa
    25021 tctcaaacgc gccgccccaa aaagaccccg gcgataacaa aatatctaaa ctcaaataac
    25081 agtttctgcc catatactgc tgttcaaaac ggcaccaaaa aacgccgctt gtatctccct
    25141 gttcttttgc tttgtcatag atacggcaaa aaaccgaaga atgtttagaa ccgatggtaa
    25201 gggttttgcc tttttcagta gggttaagcc agtcatcccc gagcttgccg acaagcggac
    25261 gtttcccgcg tttatcaaac ttgccctgtt cgtacatttc ccaagcggta tcaggcgaga
    25321 tttcattttc gtaaaagtct tttgcaacgt cgcaacgggt tatttttgcg ttataagtcg
    25381 tatccgccgt taaaaaccta tacaaacgtt cttcccagcc ttcctccgca actgcacaac
    25441 ctttacccgt catctctaca agtatggttt cctgttgtcc accgatgtaa acctgaccgt
    25501 acaaaactcc gtctctttcc atctgccagc gcgactcata aaaccgacct ttgccgacag
    25561 gttgatgcga cgaaattccg aaaccgaata tccattcaag aatgtctgaa aaacgaacta
    25621 atacatccct atctgatacg cctttaatcg gaaaaaattc ccttgttatt aaaaaaaact
    25681 ttgcaacaga actttcatga aacgtaaagc tgattgtgtc tataaatgcg gaatcgcctt
    25741 tgccacgcct taagggaaca acctttaaat ttccctttgc atccataaca accttttcat
    25801 aacgctcgta ctcttgaggt tgaatgcatg aatttgtgtc tcttgaaaat tccatgtttt
    25861 agattcccgt cgcccttcga ttttcgaccc cccctgttag ataagggggg gcataaaaac
    25921 atcagttaca caagccgaac gagcggcgtg attctcgact gcccaaagct aaaaaaacat
    25981 cgcaaacgga caaagaaaca atttcgcccg attcgatata ttggcgggca gaacccaaag
    26041 ccaaaaaaag cgtatcaaat tcggcttctg ttacaaaaat tccgtatgaa taacaattaa
    26101 ccagccattt gccgtttaca aatattattt ctgctcgtcg tttgttcatt tttagaccca
    26161 tttgcacttt tacaacagat tcgagttaat gagttaatgc gttaacattt tgagtaatta
    26221 ttaaccagtt aacttgttaa gtcaatcaca aattacttgt taacactgct tgtaatgttt
    26281 taaatttgaa atatgaaagg aaattgaaaa aatgaaggca ctacgaatca aagaagagca
    26341 agaagagagc atcagaaggc ttgccatcaa tgccaacaag aagctcattc agcttggccg
    26401 cgaaccgctt agggacagcg agcttgcaca catactttta aacgaggcaa tccgatgcgc
    26461 caaaatcgac gacgacggaa aaatcactat aggtaactga tatgaaaaaa gccctgaaat
    26521 ggattgccgt ctttatcgct ttttccgttt ggtcattcgg catatttctg tacggattca
    26581 ataacgggac tgaagcagga atgcaatgcg cccaaatcaa aggtaatgga ctttgtgcaa
    26641 tgtcctttga tggttcggct tcaaaaatcg aattttcaga ccttaatgag aaaaatgata
    26701 aacaaaccct ttgctttcaa tcattatggg aatttaacga tgaatgcgaa aaaaaagaat
    26761 ctaattctat atattatata gacctacccg aaaacaatcc caaagacata aaacaataag
    26821 gagcatatgc tcaaatagag agcctttgca aaaatctgta tcattaaatt acaggttttc
    26881 gcaaaggctt tgattttatt atgctttcac aatcccaatg gctggatgcc gtcaaagggc
    26941 ggttacgggt gcgttccgat tacgcgcttt ccaaaagatg ggcggttacg ccgtccgagg
    27001 tgtcccaata tcggcgcaac cggttgcggt ttccttttgc cgtcgtcttg gatattgccg
    27061 acgtgatgga tatagatccg attgaaatta ttgcaggttt ggaatatagg cggtgcaggg
    27121 aaaaggacat ggagcgcgtc aaaggggcgt atttcaaagc ccattgcgcg cattttgtct
    27181 atccgaacaa tccgggcttt ttccgccgta aaaaacggtt tttcggctag ttcgcataat
    27241 gtatattatg ttaaattata tatttgtgca aacggtctgc ttcggattcg tccttgcgtt
    27301 acacccctaa ctccaaggca ttaagctgtt gtttgatgct ttgccagttt gggagaaagt
    27361 tgtccatatg cgccataaaa cgggcgttgc gatggcgttc gagcaaatgg gtcagttcgt
    27421 ggacgacgac gtattaagcg gtgcggacac ccgaaccgcg ccacttggcg gcaacacgct
    27481 gatgtgcagg tttttaatcg ctttcctgcg gatttctaca tttagattgc cgattttcat
    27541 attaagctcc gtattccgcg tgcctgataa tcagctcaaa cactgcatca atatccagcc
    27601 ctttttcatc aatgagcagc ttaagttgga ctttaaggcg tttttgcttc at
//
LOCUS       DAWKST010000004        26564 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.004, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000004 DAWKST010000000
VERSION     DAWKST010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26564
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.004"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            1182..2630
                     /gene="gnd"
                     /locus_tag="WJL87_000104"
     CDS             1182..2630
                     /gene="gnd"
                     /locus_tag="WJL87_000104"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ7798601.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTGIIGAYSLQDLVDKLEKPRKIMMMVRAGSVVDDFIEQLLPLLEKGDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGMGVSGGEEGARHGPSIMPGGDERAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2693..3964
                     /gene="waaA"
                     /locus_tag="WJL87_000105"
     CDS             2693..3964
                     /gene="waaA"
                     /locus_tag="WJL87_000105"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ7798602.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRGERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGVLMETEIWPNLMKECRRAGVPLFLANARLSEKSLN
                     GYLKVRSLIRPAVASLTGCLVQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGDRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTAFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKTLSYEGGGMQMQ
                     ARVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            4005..4454
                     /locus_tag="WJL87_000106"
     CDS             4005..4454
                     /locus_tag="WJL87_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ7798603.1"
                     /translation="MSIYALAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VEKAMSYRAVRVMPFVVGLLFASGVVMAANRYLPILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            4487..5461
                     /locus_tag="WJL87_000107"
     CDS             4487..5461
                     /locus_tag="WJL87_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ7798604.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNLGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVRQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEEDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQPDK"
     gene            5530..5605
                     /locus_tag="WJL87_000108"
     tRNA            5530..5605
                     /locus_tag="WJL87_000108"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5563..5565,aa:Lys,seq:ttt)
     gene            5825..7078
                     /gene="murA"
                     /locus_tag="WJL87_000109"
     CDS             5825..7078
                     /gene="murA"
                     /locus_tag="WJL87_000109"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ7798605.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGV
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            7159..8337
                     /locus_tag="WJL87_000110"
     CDS             7159..8337
                     /locus_tag="WJL87_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ7798606.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     QYCMDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKNVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFTADAEAV
                     VKDIADVAEDDMILDIGPKSAAELADLLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGA
                     "
     gene            complement(8404..8652)
                     /locus_tag="WJL87_000111"
     CDS             complement(8404..8652)
                     /locus_tag="WJL87_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ7798607.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(8743..9660)
                     /gene="ftsX"
                     /locus_tag="WJL87_000112"
     CDS             complement(8743..9660)
                     /gene="ftsX"
                     /locus_tag="WJL87_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ7798608.1"
                     /translation="MSIIHYASLHIESARTALKELLRQPFGTLLTLLMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLARDKRIANVRFIGKEDG
                     LAELQSNLDQNLVSMLDGNPLPDAFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSTVRPLVDAIFKPYGLNIGWRFFYPEEIA
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            complement(9657..10307)
                     /gene="ftsE"
                     /locus_tag="WJL87_000113"
     CDS             complement(9657..10307)
                     /gene="ftsE"
                     /locus_tag="WJL87_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ7798609.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKVHFNNQDLGTLTDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(10501..10983)
                     /locus_tag="WJL87_000114"
     CDS             complement(10501..10983)
                     /locus_tag="WJL87_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ7798610.1"
                     /translation="MKYLNLAAIALAATFAAHTASADELAGWKDGTAQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKTYGNTVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            11107..11460
                     /gene="arsC"
                     /locus_tag="WJL87_000115"
     CDS             11107..11460
                     /gene="arsC"
                     /locus_tag="WJL87_000115"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ7798611.1"
                     /translation="MPEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPDLDNDALLRAIAEHPALLERPIVLANG
                     KAAVGRPLENIEAIL"
     gene            11457..12113
                     /locus_tag="WJL87_000116"
     CDS             11457..12113
                     /locus_tag="WJL87_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ7798612.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIDSVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            12229..13623
                     /locus_tag="WJL87_000117"
     CDS             12229..13623
                     /locus_tag="WJL87_000117"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ7798613.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLNYDNADNVVYQTRRFDRYKEVIAELLEKGHAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWADL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITTNEKLAEMVKPRLA
                     LRDIHETSKPALEDVLALVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEASAR
                     MLRFAERLEGLEDWNTEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            13644..14039
                     /locus_tag="WJL87_000118"
     CDS             13644..14039
                     /locus_tag="WJL87_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798614.1"
                     /translation="MPSDVGIRLQTAFKRKLKMKKIFYFLMVVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNAVYGAGGTKASGN"
     gene            14043..14534
                     /locus_tag="WJL87_000119"
     CDS             14043..14534
                     /locus_tag="WJL87_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ7798615.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIH
                     AYYGRCLKDDELPLAFAAVDDSGILLGSATVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDM
                     DKV"
     gene            complement(14657..17251)
                     /gene="mutS"
                     /locus_tag="WJL87_000120"
     CDS             complement(14657..17251)
                     /gene="mutS"
                     /locus_tag="WJL87_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEZ7798616.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKNIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSANGSSLLETLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            17695..18699
                     /gene="gap"
                     /locus_tag="WJL87_000121"
     CDS             17695..18699
                     /gene="gap"
                     /locus_tag="WJL87_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ7798617.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPSGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(18769..18888)
                     /locus_tag="WJL87_000122"
                     /pseudo
     CDS             complement(18769..18888)
                     /locus_tag="WJL87_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18957..19637)
                     /gene="tsaA"
                     /locus_tag="WJL87_000123"
     CDS             complement(18957..19637)
                     /gene="tsaA"
                     /locus_tag="WJL87_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ7798618.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLVSAAEVCIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLNRIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEIRFQIEENRATV
                     IDLSPTPL"
     gene            19752..20387
                     /locus_tag="WJL87_000124"
     CDS             19752..20387
                     /locus_tag="WJL87_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEZ7798619.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMVAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGNWQVIVNEAACRGIAPDTIETAWQEMSSAGAVILKNAEEIKKYINNQ"
     gene            20425..21393
                     /gene="waaC"
                     /locus_tag="WJL87_000125"
     CDS             20425..21393
                     /gene="waaC"
                     /locus_tag="WJL87_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ7798620.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFTDI
                     ARLHPFVKKIHVMKWRQWRKHLFQAETWREMGHLKQTLRQEAFNFVLDSQGLIKSACF
                     AKMAKSPIYGLDKNSAREGLAALAYAKTYAVPKGKNAVWRNRELFAQVFGYAMPETQV
                     FGLTVPEVGRLKNLAQPYYAALHATSRDSKLWPMENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQTLMDCVAADKG"
     gene            21619..22368
                     /locus_tag="WJL87_000126"
     CDS             21619..22368
                     /locus_tag="WJL87_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ7798621.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDTKLESLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVHIECGEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLTPDDLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            22379..23314
                     /locus_tag="WJL87_000127"
     CDS             22379..23314
                     /locus_tag="WJL87_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ7798622.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKIDLLVAGSGASAVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAVDYCYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRATVFDAVAAQGGN
                     AEVINVEATPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEVLKN"
     gene            23377..23988
                     /locus_tag="WJL87_000128"
     CDS             23377..23988
                     /locus_tag="WJL87_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ7798623.1"
                     /translation="MITTYKTITSSTQAEFKDKGSRFIAFTYPIRTLADVKKYLDLLK
                     EEHHKARHWCYAYRLGVDGVQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     ILERDLQLDCRLTVSLSLVNYEACVAAWKNTRQIEVNTEKPFE"
     gene            complement(24040..24357)
                     /locus_tag="WJL87_000129"
     CDS             complement(24040..24357)
                     /locus_tag="WJL87_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ7798624.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKYVVTLIRDMDNFYT
                     HEYQACHQAIEDGVELDLSTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            24515..25786
                     /gene="purD"
                     /locus_tag="WJL87_000130"
     CDS             24515..25786
                     /gene="purD"
                     /locus_tag="WJL87_000130"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ7798625.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGIVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDIISGLDAANQIGKVFHAGTTAKEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            25820..26422
                     /locus_tag="WJL87_000131"
     CDS             25820..26422
                     /locus_tag="WJL87_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ7798626.1"
                     /translation="MTDVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            complement(26494..>26564)
                     /locus_tag="WJL87_000132"
     CDS             complement(26494..>26564)
                     /locus_tag="WJL87_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ7798627.1"
                     /translation="EFRFNFGTPSQQLKILRDWCGI"
BASE COUNT         6391 a         6102 c         7469 g         6602 t
ORIGIN      
        1 ttggttttct gtttttgtgg gaatgacggg atgtaggttc gtaggaatga cgtggtgcag
       61 gtttccgtat ggatggattc gtcattcccg cgcaggcggg aatctagaac gtaaaatcta
      121 aagaaaccgt gctgtaaacg gcagactgat gccgtcattc ccacgaaagt gggaatccag
      181 aacgtaaaat ctaaagaaac cgttttcccg ataagtttcc gtaccgacaa ggctggattc
      241 ccgcctgcgc gggaatgacg gcggagcggt ttctgttgct cccgataaat tcctaaaact
      301 taaattttca tcattccctc aaaaacagaa aaccaaaaac agaaacctaa aatcccgtca
      361 ttcccacgaa agtgggaatc tagaacgtaa aatctcaaga aaccgttttc ccgataagtt
      421 tccgtaccga caaggctgga ttcccgcctg cgcgggaatg acggcagagc ggtttctgtt
      481 ttttccgata aatgccgcaa tctcaaatcc cgtcattccc actttcgtgg gaatgacgga
      541 atgtaggttc gtgggaatga cgcggtacag gtttccgtta cgtatcccca tccgaaagtt
      601 tatttataca caagcaatcc aaagttatct gactgaaata ttgtgagatt tacccgcaaa
      661 tctcattttt tccaccactt atacacaaat tcggatgcgt actctgccaa tatgcagaaa
      721 aaagatattt gtatcgacac attctcattc ctacaagttc caaacacact tccctctcaa
      781 atctccacaa cattcccgtt cttaaaggca tcggcagcca ttcggtaaag aaaagcggaa
      841 tgccatctgc acaacaagca aactgtccac aaaaactatt cttcataaaa aacaatccac
      901 aggtatgcac aaaagggaat ccgccttacc gacacccttt taaaacggaa atcccaatga
      961 tttttaaaat aaaaaaaagt tacccacaga aaatttctcc tatcaatata atcatcgttt
     1021 ttttatttaa tttttatatt ttatttataa gccttcaggg aaacgcacgg taaagcgtaa
     1081 aggcatcttt tcaaaaaatt aaaaagttaa ggattgttaa acaagtgtaa atcaggtaga
     1141 gttgcggtcg ataaattaaa tctataacgg agcttcctgc aatgaaaggc gatattggcg
     1201 taatcggttt ggcggttatg gggcaaaacc tgattttgaa tatgaacgat tgcggattta
     1261 aggttgtcgc ctacaaccga acaatcggta aggtggacga atttttaaac ggcgcggcaa
     1321 aagggacggg cattatcggc gcgtattccc tgcaagactt ggtcgataag ctggaaaaac
     1381 cgagaaaaat catgatgatg gttcgcgcag gttcggtggt tgacgacttt atcgaacaac
     1441 tgcttccgtt gttggaaaaa ggcgacatca ttatcgacgg tggcaatgcc aattaccccg
     1501 acacgacacg gcggacacat taccttgccg aaaaagggat tttgtttgtc ggcatggggg
     1561 tctccggcgg agaggaaggt gcgcggcacg gcccgtctat catgccgggc ggagatgagc
     1621 gcgcatggga agctgttaaa ccgattttcc aggcgattgc tgcgaaaacc cctcaaggtg
     1681 agccgtgttg cgattgggtc ggcagggatg gtgcgggaca tttcgtcaaa atggtgcata
     1741 acggcatcga atacggcgat atgcagttga tttgcgaagc gtaccagttc atgaaagacg
     1801 gtttgggact gtcctacgac gaaatgcacc gcgtgtttgc cgagtggaac aaaaccgagc
     1861 tggattctta tctgattgaa atcacagcgg caattttggg ctataaggac gaaggcggcg
     1921 aaccgctggt cgagaaaatc ctcgatacgg cagggcaaaa aggcacgggc aaatggacgg
     1981 gcatcaatgc tttggatttg ggtattccgt tgacgctgat ttccgaagcg gtattcgccc
     2041 gctgcgtttc gtcgttcaaa gaacagcgcg tgcagaccgg caagctgttc gcccgaaccg
     2101 ttacgcccgt cgaaggcggc aaacaagaat gggttgaggc gttgagacag gccctgcttg
     2161 catccaaaat catttcctac gcacaaggct ttatgctgat ccgcgaagcg ggcgaaagct
     2221 acggctggga tttggactac ggcaacactg cgctgctgtg gcgcgagggg tgcatcattc
     2281 gcagcgcatt cttaagcaat atccgcgacg cgtatgaaaa caatcccgat ttggtgttct
     2341 tgggtgcgga tccgtatttc aaaaatattt tggaaaactg cctgccggcg tggcgcaagg
     2401 tggttgctaa ggcggtcgaa tgcggcatcc ctatgccttg catggcttcg gcgattacgt
     2461 tcttggacgg ctacacgacc gaacgtctgc ctgccaatct cttgcaggca cagcgcgact
     2521 acttcggcgc gcacacttac gaacgcaccg acaaaccgcg cggcgagttt ttccatacca
     2581 actggacggg caagggcgga gataccgctt cgacaaccta cgatatttga ttccgttgcc
     2641 gaaatgccgt ccgaaccgtt cggacggcat ttgttttgca ggaaatagaa agatgttcca
     2701 atggctttat gatgtattgt ggctgcttgc gccgatatgg atacggcgtt atttggacaa
     2761 acgctccgga agtgccccgg catatcgggc gcatcggggc gagcgtttcg gcaagccgta
     2821 tccgaatccc gttaccggcg cggtttggat acacgccgtt tcggtcgggg aaacgcgtgc
     2881 cgcccaacct ttgatacgcg agttgcggca gcgttttccc gatgcgccgc tgctgatgac
     2941 gcagatgacc ccgacgggac gggaaaccgc gcaagttctg tttcccgatg cgcaatgccg
     3001 ctatcttccg tatgacaaaa aaacgtgggt acggcagttt ttgcgcgaac accgcccgat
     3061 gttcggcgta ttgatggaaa cggaaatctg gcccaaccta atgaaggaat gccggcgcgc
     3121 gggtgtgccg ctgtttttgg cgaatgcgcg gctgtcggaa aaatcgttga acggttatct
     3181 gaaagtccgc agcctgatcc gtcctgctgt cgcttcgctg acgggctgtc tggtacagac
     3241 ggaggcggat gcggcgcggt tggcgaaatt gggcgcggca tccgtgcagg tgtgcggcaa
     3301 taccaaatac gacctgatgc cgtcggaaca gatgaaaacg ctggcggggc agtttgaaaa
     3361 acgcatcgga gaccggccgg ttgccgtgtg cggcagcacg cgcgtttatc ggggtgaaga
     3421 cgaggcggag aaactgctgg cggcgtggca acaatatcgc ggcgatgccc tgctggtcgt
     3481 cgtgccgcgc catcccgagc attttcagac ggcatttgaa acggcaaaac gcttcgggtt
     3541 taaggttcag cggcgcagcg acggtttgcc ggtcgaaccc gatacgcagg tgtggatagg
     3601 cgacagtatg ggcgagctgt atgcgtatta cctgtgcgcc gatgttgctt ttgtcggcgg
     3661 cagtctggtc gattcgggct gtcagaacat catcgaaccg ctttcctgcg gcgttccgac
     3721 gatattcggc ttttcgacct acaatttttc cgaagcctgc cggcacgcct tggcatcggg
     3781 tgcggcggtt caagtcgaat cggcggacgc gtggcgggaa gccgttgaaa aaaccttatc
     3841 gtacgagggg ggcggaatgc agatgcaggc gcgcgtggac ggctttatcg cacaacatcg
     3901 cggagcgggc gcgagaatcg ccgaggcggt gcgggaagcg gtatgcggac atcgggggcg
     3961 atagtgatac aatccgcatc cgagttttcc ggttggagta ccgtatgagt atttacgcac
     4021 ttgcacacat cgtccatctg tattgcgcca ttgcctttgt cggcggcgtg ttttttgaag
     4081 tgctggtttt gtccgtcctg catacgggac gggtgtcgtg cgaggcgcgg cgcgaagtgg
     4141 aaaaggcaat gtcttaccgc gccgtcaggg tgatgccgtt tgtggtcgga ctgctgttcg
     4201 ccagcggcgt cgtgatggcg gcaaaccgct accttcctat attgggcgaa ccgtttgcca
     4261 cttccttcgg tacgatgctg acgctgaaaa tcctgttggc gttcagcgtg ttggcgcact
     4321 tcgccatcgc cgtcgtcaaa atggcgcgtt ccacactgac ggtcggctgg tcgaaataca
     4381 tacacgccgt cgtctttacc catatgctgc tgattgtctt tttggcaaaa gcgatgtttt
     4441 atatcagctg gtaacacaac tacatttcaa atcaggagat tgaaccatga cagaatatcc
     4501 cggcatcggg tcgccgttgt tttacggcgt tttttttgcg gcagtgctgg tcatgattgc
     4561 cttggatatg ttttcgctga agaaaaacgg cagccataaa gtcggcgtca aagaagcctt
     4621 ggcatggagc ggcttgtggg ttgccgtatc ctgcctgttc gcgggctggc tgtatttcga
     4681 actcgccggc aatctcggct acggcgcggc agtcgcaaaa gaaaaagtat tggaattctt
     4741 taccggctac attttggaaa aatcgttggc agtcgacaat attttcgtgt tcctgatgat
     4801 attcggctac ttcaaagtcg caccacagtt tcagcaccgc gtgctgctgt acggcgtatt
     4861 gggcgcattg gtattgcgca ctgtcatgat tttcgtcggc gcggcactgg ttcggcagtt
     4921 cgagtggatt ctgtatctgt tcggcgcgtt cctgctctat accggcatac acatgatgaa
     4981 gcccgaaggc gatgaggaag aggatttggc aaacagcagg ctgctgaatg ccgtcaagaa
     5041 agtcgttccg gtcggcacgg aatttcacgg cgagaaattt tttaccgtcg aaaacggcaa
     5101 aaaaatcgcc acgccgctgt ttttggtgct agtcatgatt gaattgagcg atgtcgtgtt
     5161 tgccgtggac agcatccccg ccgtctttgc cgttaccacc gatccgttta tcgtgctgac
     5221 ctccaatatt ttcgccattt tgggtttgcg ggcgatgtat ttcctgctgg cggatgtggc
     5281 ggaacgcttt atcttcctga aatacggctt ggcattcgtg ttgggtttta tcggtgtgaa
     5341 aatgctggta atgcattggg tgcatatccc gatttccgtt tcactgtcgg tcgtgttcgg
     5401 cgcgttgggt gcatcgatac tgacctcgtt aatttatacg aaaaaacagc ctgataaata
     5461 aaatgccgtc tcaacgccga caaaatggtt gacagcatca ggcttgatgt atagaatttc
     5521 gagcttgtcg ggtcgttagc tcagtcggta gagcagcgga cttttaatcc gttggtcgag
     5581 cgttcgaatc gctcacgacc caccagataa aacaaaaaaa gctgagtttt caaaactcgg
     5641 ctttttttgt tttccgacaa tccttgttcg gaaagcgcgg catttcgtca aattctttgg
     5701 tgcggcttcc gtatttttcc cgaaaatccc ttataattcc gcctctgcaa ccttcatcgg
     5761 gtgatgcttt atgcgttgtc ccgagaattg aaatattaca agtacccacc aggaaagaac
     5821 aaacgtggac aaactgaaaa tctccgcaaa cggcccactc aacggggaaa taacggtctc
     5881 gggcgcgaaa aacgcggcat tgccgctgat gtgcgcgggt ttgctgacat cgggtacgtt
     5941 gcgcctgaaa aacgtcccta tgctggcaga tgtgaaaacc acgcaaaagc tgcttcaggg
     6001 gatgggcgcg cgcgtcctga ccgacaatat cagcgaattt gaaattaacg gcggcacggt
     6061 aaacaatacc tgcgcccctt acgaattggt acggacgatg cgcgcctcga ttttggtgct
     6121 gggcccgacg ctggcgcgtt tcggcgaggc gcaagtcagc ctgccgggcg gctgcgccat
     6181 cggttcgcgc cccgtcgatc agcatttgaa gggcttggaa gcgatggggg cggaaatcgt
     6241 catcgaacac ggttacgtca aggccaaagg caaactcaaa ggtacgcgcg tggcgatgga
     6301 tgtcgttacc gtcggcggca cggaaaacct gctgatggcg gcgactctgg cggaaggtac
     6361 gacggttttg gaaaactgcg ccatcgagcc cgaggtggtc gatttggcgg aatgcctggt
     6421 taaaatgggc gcgaaaatca gcggcatcgg aacatcgacc atgatcgtgg aaggtgtgga
     6481 cgagctgcac ggctgcgaac acagcgtcgt ccccgaccgg atcgaggcgg ggacgttcct
     6541 gtgcgcggtg gcgataaccg gtggcagggt ggttttgcgg aatgccgcgc cgaaaacgat
     6601 ggaagtggtg ttggacaaac tggttgaggc aggtgcggtg attgaggcgg gcgacgattg
     6661 gatcgccatc gatatgcggc agcgtccgaa ggcggtggac atccgcacgg tcgtccaccc
     6721 cggcttcccc acggatatgc aggcgcagtt tatggcattg aatgccgtgg cggagggaag
     6781 ctgccgcgtg gtggaaacga tttttgaaaa ccgctttatg cacgtccccg agttgaaccg
     6841 gatgggggcg aacattacaa ccgagggcaa tacggcattt gtgcagggtg tggaacggct
     6901 ttccggcgca gtcgtcaagg cgacggattt gcgtgcgtcc gcaagcctcg ttatcgccgg
     6961 tttggcggcg cgcggcgaaa ccgtggtcga acagatttac cacttggatc gcggttatga
     7021 aaatattgaa aaaaaactcg gcagcgtcgg cgcgaaaatc gagcgcgtat ccggctgaac
     7081 gggttgcgat gccgtctgaa gccttcagac ggcatcaatt gtttctgtat cgcaaacact
     7141 gattgaaaag gactgattat ggcattttta aaactgaccg aacaaaacgt gcagggcaaa
     7201 accgtcctca tccgcgccga tatgaacgtg ccgttcaaag acggcaaaat cagcgacgac
     7261 acccgtatcc gcgcctcgct cgcgtccatc caatactgca tggacaacgg cgcatccgtt
     7321 atcgtgatga cccacttggg ccgcccgacc gaaggcgagt tccaccccga agacgacgtt
     7381 gcgcccgttg ccgcgcattt gggcggcctg ttgggcaaaa acgtgaaagt attgaacgac
     7441 tggcgcgaaa acaaaccggc tttgaacgcg ggcgatgtcg tgatgctgca aaacgtgcgt
     7501 atcaacaaag gcgagaagaa aaacgatttg gaactgggca aagcctacgc ttctttgtgc
     7561 gacgtgttcg tcaatgacgc gttcggcacc gcgcaccgcg cccaagcctc gaccgaagcc
     7621 gtcgcccaag ccgctcccgt tgcctgcgcc ggcgtattga tggcgggcga actcgacgct
     7681 ttgggcaaag ccttgaaaca gcccgcgcgc ccgatggttg ccatcgttgc cggcagcaaa
     7741 gtgtccacca agctgaccat cctcgaatcg ctggcggaca aagtcgacca actcatcgtc
     7801 ggcggcggca ttgccaacac cttcctgttg gcggaaggca aagccatcgg caaatctttg
     7861 gcggaacacg atttggtgga agaatccaaa aaaattatgg caaaaatggc ggccaaaggc
     7921 ggttctgtgc cgctgccgac cgatgtcgtc gttgccaaag cctttaccgc cgatgccgaa
     7981 gcggtcgtga aagacattgc cgacgttgcc gaagacgata tgattttgga catcggcccg
     8041 aaatccgccg ccgagcttgc cgatttgctc aaagctgccg gtacggttgt atggaacggc
     8101 cctgtcggcg tgtttgagtt cgatcaattc gcaggcggca cgaaagctct tgccgaagcc
     8161 attgcccaaa gcaaagcatt ctcgattgca ggcggtggcg acacgctggc ggcgattgct
     8221 aaattcggcg ttaccgacca aatcggctat atttctaccg gcggaggcgc gtttcttgag
     8281 ttcttggaag gtaaagagtt gcctgccgta gccgctttgg aaaaacgcgg cgcgtaagcg
     8341 gtttgatata aaaaatgccg tctgaaacgt tgtgctgttt ctgacggcat ttttgtgtgg
     8401 cgattattgt gctttggctg cccattccgc cggagtggcg gcaaccgaaa tggaaagtgc
     8461 gtgcagttcg ttactttcca gttgggcttt gagtccgtct ttaatcaggc ggtggcgcgc
     8521 gaggcgtgcc ttgccttcaa attctgatga aacgatgacg gcgaaaaaat ggtgtccgtc
     8581 gccttctact tcgatatgtt cgcattttgc cacgccttca atcatggctt ttacttgttc
     8641 cgaggtcagc atggtaggtt ccttgttgga aaaatgcgcc attatactgg caaattgaaa
     8701 tgccgtctga aaacgggttc ggacggcatt tttgacggtg ttttattttt tggctttgaa
     8761 gccgagcagg tgctgggtgg tggcaagcca cgcgccgaat acgcccaacg cgatgacgaa
     8821 gccgaacacc agcgcgattt cttcaggata aaagaaacgc cagccgatat tgagcccgta
     8881 gggtttgaag atggcatcga ccaaggggcg cactgtggaa agcagccaac cgcaaagccc
     8941 caggctgacg gcggcggaaa ggatgctctg ccacattgcc tgataaagga aggggcggcg
     9001 gatgaacgat gcgggcgcgc ccaagagttt ggtgatttcg atttcttctt tgcggctgag
     9061 gatttgcagg cggatggtgt tgtgtgcgac aaggacgaac gccataccca gcgtcaggga
     9121 aagaaaccat aaaattttac ggataaattc gttgatttgg tacagcgttt gcacccattc
     9181 ggtatccata gacgcggatt cgaccatagg cagtttggta atgtcccggt agattgcctg
     9241 catttgcgcg ggcgttgtcg cggggtcggg ggtaacgata aatgcgtcgg gcagcgggtt
     9301 gccgtcgagc atcgaaacca aattttggtc gaggttggac tgtaattccg ccaagccgtc
     9361 ttccttgccg atgaagcgga cgttggcgat ccgtttgtcg cgcgccagca ggctgcggac
     9421 ggtatcgctg tcgctttgtg cggcggcggt ttccatatag acggtgattt gcggcgactc
     9481 gttgagtttg cccaacacgc tttgcccgct ttgaatgccc aaatacataa acagcggcag
     9541 ggtcatcgcg acggcgagca tcaggagggt gagcagcgtg ccgaagggtt ggcgcagaag
     9601 ctctttgagc gcggtgcggg cggattcgat gtgcagcgag gcgtagtgga tgatgctcat
     9661 gcgagtcgtc ctttcgagag gcgcaggatg cggtgtccgt agtccgccat cagggtttcg
     9721 tcatgggcgg caacgatgac ggttgttccc gcttcgtgga aggttttgaa caattccatg
     9781 atgtcgagcg cgtaggcgcg gtcgaggttg gcggagggtt cgtcggcaat cagcaggccg
     9841 ggctggtgaa cgacggcgcg ggcgatgcac aggcgttgtt gttcaccgcc ggagagggtt
     9901 acgggatcgt ccaattctcg tcctttcagg ccgacttttt cgatggcgat gcgggcgcgt
     9961 tcttcggctt tgcgcggcgg atagccgata atccgaagcg gcaggatgac gttttgcagg
    10021 acgttgcggt cgtagaggat tttgtggtct tggaacacga tgccgatgtg ttggcgcata
    10081 aagccgattt ggttgtcggt cagtgtgccg aggtcttggt tgttgaagtg gactttgccc
    10141 ctgcttggtt tggtaatgcc cgaaatcagc ttgaggacgg tggatttgcc tgaaccggag
    10201 tgtcctgcga taaaaatcat ctcgcctttg ttgatttgga agctgacgtt tttcagggct
    10261 tcaaaaccgc cgggataggt tttggaaact tgttcgaaac ggatcatagg aggtcctgta
    10321 aggaagggcg gcatatcgtt tcgggcggtt gcgtccgcag ggatatgccg tctgaacaat
    10381 aggtaagaca gtgccgatta taaagattcc gcctgctttt agatagtgga taagtttggc
    10441 ggatatgaag gtcgggcggg aaaaatgccg tctgaagcgg cttcagacgg catccggcgt
    10501 ttaacggcat tttgaatggg cgagtttgac ggcttcggtc aggctttttt cgttcacttc
    10561 cccggtaatg gtctgcctgt atccgcattt cggtgcttcg acgacggtaa agggcagtac
    10621 gccgacagtg tttccgtagg ttttcataaa gtttcggctg ttcgccccgg tgtaacgcca
    10681 aatcgggtag gaaacaggag tttgtttgag gaagttgccg atattgtcgg atgtgtcgag
    10741 cgcgatgccg accatatcga cgctgccttt tttctgcgct ttgtaccatt tggacatggc
    10801 gggcatctct tttcggcacg ggccgcacca agtcgcccac aggttgacga tgcgtacggg
    10861 ggctttgagc gattgcaggc tttgcgcggt accgtctttc catccggcaa gttcgtctgc
    10921 cgaggcggta tgtgcggcaa atgtggcggc aagggcgatt gcggcaagat tcaagtattt
    10981 catcgttttg tcctttgtat gttgattcgg gtattgtaaa ccgtttggta cggttggggg
    11041 cgggatattg agtaaaaccc tgcgtaagcc gataataagg ggattgttga gaagcaagga
    11101 aatgtcatgc ctgaaatcaa aattttccac aatccgcgtt gcagcaaatc gcgtgccgcc
    11161 ttgtccctat tggaagaacg cggcattgct gccgaagtgg tcaaatattt ggatacgccg
    11221 cccgacttgt ccgaattgaa ggatattttc aacaaattgg gcttggcatc ggcgcgcggg
    11281 atgatgcgtg tgaaagatga tttgtacaag gaattgggtt tggacaaccc cgatttggac
    11341 aatgacgcgc tgctgcgtgc catcgccgag catcccgccc tgttggagcg tccgattgtt
    11401 ttggcgaacg gtaaggcggc tgtcggcagg cctctggaaa atatcgaagc gatattgtga
    11461 acaaacggct gttttgcagc cgcaacggct tgcgctatta cctgttgggc ggtttctgtt
    11521 tgagtgtttt tccgcttttg ctggtgtttg cctcatccgt ttgggcggtt tatcggacgg
    11581 gcgggcaggt tttgccgccg tatgtccggg cggatgccgc gctggtgttg ggcgccgccg
    11641 cgtgggacaa acgcccttcc ccggttttcc gcgaacgcat caaccacgcc atcgcgcttt
    11701 atcagagccg cagggtaggc aaaatcgtgt ttacgggagg gcgaaccaaa aaaggctata
    11761 tgaccgaagc cgaggtcggg cggcggtatg cgctcaaaca gggggttccg gcgcgcaaca
    11821 tcctgtttga aaacacttcg cgcaacactt atgaaaacct gaacaacatc cgtccggttt
    11881 tgcgtgccaa cggtattgac agcgtggtga ttgtcagcga tccctaccat ttggcgcggg
    11941 cggcggaaat ggcggaggat ttgggtgtcc gcgtttatat gtctgccacc ccgaccacgc
    12001 ggtttgatgc gggaaataag aaaaaaatct ttatgctgca agagggatat gcgttatccc
    12061 tctaccgttt ggaaaaatgg ggcagccgct ttttgggatg gctgtctgat tgaagcggta
    12121 gaaaatgccg tctgaaactt atccgcaggc agggttcgga cggcattttt gatgcccaag
    12181 cacggtaaaa ccagtaaaat aaacgctttc aaagatttcg gagccatcat gaccgtcaaa
    12241 acccgtttcg cccccagccc caccggctac ctgcacattg gcggcgttcg caccgccttg
    12301 ttttcgtggg cgtttgcccg ccatcataaa ggcgaatttt tattgcgcat cgaagacacc
    12361 gacttggcgc gttctaccgc cgaatccgtc aacatcatcc tcgacggcat gaaatgggtc
    12421 ggcctgaact acgacaacgc cgacaatgtc gtgtaccaaa cccgccgttt cgaccgctac
    12481 aaagaagtca ttgccgaact tttagaaaaa ggccatgcct actactgcta ttgcagcaaa
    12541 gaagagctgg aagccatgcg cgagaaagcc gaaaaagaag gcacggcgac ttacgaccgc
    12601 cgctggcggc cggaagcggg caaaaccctg cccgaaatcc ctgccggcgt gcaacccgtc
    12661 gtccgcttca aaacgccttt ggatggcgta accaagtggg cggacttggt caaaggcgaa
    12721 atttccatcc ccaacgaagc cctcgacgac ctgattatcg cacgcgccga cggcacaccg
    12781 acctacaact tctgcgtcgt ggtggacgac tacgacatgg gcgttaccca cgttatccgg
    12841 ggcgacgacc atgtgaacaa caccccgaaa caaatcaaca tcttaaaagc catcggcgca
    12901 aacctgcccg aatacggaca cctgcccatg attctcaacg aacaaggcaa aaaaatctcc
    12961 aaacgcagcg gcgataccgt cgccattacc gatttcggcg caatgggcat cctgcccgaa
    13021 gccatgctca actatctggc gcgcttgggc tgggcgcacg gcgacgacga gttctttacg
    13081 atggaacaat tcatcgaatg gtttgatttg aaagacgttt ccccgtctcc aagccgtatg
    13141 gacttgaaaa aactctactg gatcaacggc gaacacatca aaatcaccac caacgaaaaa
    13201 ctggccgaaa tggtgaaacc gcgcctcgcc ctgcgcgata tccatgaaac aagcaagcct
    13261 gctttggaag acgtgttggc attggtcaaa gaccgcgccc aagatttgaa cgccttggca
    13321 gacgaatgcc tgtatttcta caaaaaacaa gtccctgccg aagccgatgt tgccaaacac
    13381 tgggacgacg aagcctccgc ccgtatgctg cgctttgccg aacgcctcga gggtttggaa
    13441 gactggaata ccgaagccat ccacgacctg ttcaaacctt tctgtgacga agaaggtatt
    13501 aaaatgggca aactcggcat gcctttgcgc ctcgctgtct gcggcacggc gaagacccca
    13561 agcgtcgatg ccgtgttggc attaatcggc aaagaagaag tgttgaaacg catccgcgct
    13621 taagttatcc acaggcgtgg gtgatgccgt ctgatgttgg aatacggctt cagacggcat
    13681 ttaaacggaa gttaaaaatg aaaaaaatat tttattttct gatggttgtt ttttctacaa
    13741 gcgtatgggc cggggatgcg gaagacaatc tgctcagcat ccaatccggt tatcgtgcct
    13801 tactgcaaaa acaaaacaat ctggacggaa aaatcatcgg gatgcagtcg gatttggaag
    13861 atgcgcgcag acggttgcag gcggctcagg cagacatcgc ccgtttggaa gcggaaattc
    13921 ctgcagcaat ggcgcaaaaa gcccggcagg ctgaagagct gaggcaaatc ggagtgcgtt
    13981 tggaccatgc ttggaatgcg gtttacggcg cagggggaac gaaagcgtcg gggaattgac
    14041 cgatgaattc cctctttgtg gacaatactg ttttcattac acggctgaaa gccgggcata
    14101 tcggcaggtt ggttcaggcg ttgtttgagg agtggcacgg atttgaaccg tggtcttctg
    14161 tggataagat tcatgcctat tacggcaggt gtttgaagga tgacgaactg ccgctggcat
    14221 ttgcggctgt ggatgattcc ggaatcctgt tgggttcggc tacggtcaag cggcatgata
    14281 tggaaagttt tccacagtat gaatattggt tgggtgatgt ctttgtttta cctgaatatc
    14341 gcggaaaagg cattggcagg aggctggtcg cccattgcat aggcgcagcg cgttcgctgg
    14401 ggataaagtt cttgtatctt tatacgcctg atgtgcaaat attttatgaa tcattcggct
    14461 gggcggttgt cgggcgacat ttccataacg gtgaatgggt tacggttatg cgtttggata
    14521 tggataaggt ttaagccgaa tggacaaatg ccgtctgagc tgttcggacg gcatttttat
    14581 gtttggaaac ttcttgtcag ataatgttta tccacagatt tttccatatg gtttgatgct
    14641 gttccgccaa cggaaattaa gatacggatt tgcacaaatc tttcagacgg tacagttctg
    14701 acaatgcttc gcgcggggtc aggctgtcgg gatcgagttt ttccaaggct gctgccaata
    14761 taccttcaaa atgtttttcc tctgctttgt ccacaaagtt gcccacattc ggttcatctc
    14821 ctttttcaga cggcatggta ctgaaaatat ccagttgggg acggtttgcg gcggcttggt
    14881 tttccagtcc gttcaaatgc ttttgggcgg atttcaatgc gcgtacaggc aggccggcga
    14941 gtttggcaac ggcgatgccg tagcttttgc cggcgggacc cggttggatt tggtgcagga
    15001 aaacgatgtc ctgtccctgt tcgagcgcgg aaaggtgcat attgacggcg gcggcgtggg
    15061 cttcgggcag gtaggtcagc tcgaaatagt gggtagcaaa caggctgaag gatttgtttt
    15121 tttgcagcag gtgttcggca acggcgtgcg cgagggcgag gccgtcgaaa gtggaagtac
    15181 cacgtccgac ttcgtccatt aaaacaaggc tttgttcggt ggcgtgatgc aggatgtagg
    15241 cggtttcgct catttcgacc atgaaagtgg agcggttgga ggcgaggtcg tccgatgcgc
    15301 cgatgcgggt gaagatttga tcgatgggcc cgattgtggc ggcatcggca ggcacaaaac
    15361 agccggtgtg tgccaataaa acaatcagcg cgacttggcg catgtaggtg gatttgccgc
    15421 ccatattggg gccggtgagc agcatgaggc ggtgtttgtg gtcaaggtcg gtgtggttgg
    15481 cggtgaagtg gcgtacctgc tgttcgacaa cgggatggcg gccgttttcg atgtggataa
    15541 ccggatagtc ggcaaactcg gggcggacga agttccgctc ttttgccaag gctgaaaatg
    15601 tggacaacac gtccagcgcg gcggcggctt tggcggcttt ttgaagctgc ggcaatgccg
    15661 tctgaaggtt tttcaatacg ccgtcaaaga gttgtttttc taaggcgagg gcttgctctt
    15721 gagcagtcag cactttgtct tcaaaggctt tcaactccgg cgtgatgaag cgttcggcgt
    15781 ttttgagggt ttgccggcgt tggtagtcgg caggtgcttg ttcggcttgg gttttggaca
    15841 attcaatgta aaagccgtga acgcggttga actcgacttt gagtgtggac aaaccggtac
    15901 gttcgcgttc cttggcttcc aaatccagca aaaattcgtc gccatggttt tgaatgcggc
    15961 gcaattcgtc cagttcggga tgaaaaccgt ggttgatgac attgccgtct ttcagccaga
    16021 cggaaggttc gggcaaaatg gcttggcgga gctgttcggc tgtggatagg ttttcaggga
    16081 aaacggcttt gagggtttcc aacaggctgc tgccgtttgc ggacaagtcg atttgtgcca
    16141 actcgaacag actgtcgcgt aaagatgcca aatcgcgcgg gcgggcgttg ccgacggcga
    16201 tgcgggcagc aatgcgttca atgtccgcaa tgtttttcag acggcattgg aggggtttgt
    16261 attggctttc cagcgcggca acggcttctt ggcgcgctcg gatgtgggcg cggttgcgta
    16321 aagggtggtg cagccagaga gccaagaggc ggctgcccat atgggtagcg caaaggtcga
    16381 gcgtggacat cagggtcggc gattttttgc cggagagggt ttgcgtgatt tcgagattgc
    16441 ggcgcgtggc ggcatccata ccgatatatt ggctgtcggt ttcgagcgac aggccgtcca
    16501 aatgttgcgg catcaggttt tgcgtcagac ggatatagtt caacagtgca cctgccgcgc
    16561 caatcgcaac ggcgtgttct ttgccgtcca aaccgaagcc gcgcaaatcc tggcagccga
    16621 aatattccgt cagcagtttt tcccccgcgt cggcggcaaa ctgccacgcg ttcaggcgcg
    16681 taacacccga tgccgtctga agttgcggtg cgtttttact gtcaggcaac agaatttccg
    16741 ccgcctgcag gcgcgccagt tcgtcgtcca atttatccac agttgtcagc ttggttttga
    16801 attcgccgct ttgcagcgat gcccacgcca aaccgatgta ttttttgtcg ggggacacgg
    16861 caacgatgcg gttggtttcc ttgtcttcca gcaatgcgga atcggtcagc gtgccgggcg
    16921 ttacgatgcg cacgactttg cgctccaccg gtcctttgcc tgcgccgact tcgccgacct
    16981 gttcgcaaat cgccacgctt ttgcccaact tgaccaggcg cgccagatat tgttcggcgg
    17041 cgtgaaacgg cacgcctgcc attttgaccg gctcgccatc cacctgtccg cgcgtggtca
    17101 gggtaatatc caaaagtttt gccgcttcta ccgcatcgtc gaaaaacatc tcgtaaaaat
    17161 cgcccatacg gtaaaacacc agtttgtcgg tatgttgcgc tttgatgccg aggtattgct
    17221 gcatcattgg ggaaacggcg gatttggaca tggggctgcc ttatcattag gaaaacggta
    17281 ttgtagcaaa aggttttgtc ttcgacggaa tgagactgac cgtaagcctg tcaataaggt
    17341 tgtggagaca actaggaaaa cagcaaagtt atccacagaa atattattaa gtcaaaaaac
    17401 atccacgctt atctgtctta tacagcaagt cttaaactca aacttatttg acaaaaaggt
    17461 aatgaaaata ttggatattt gctacttacg cacaagcagg ttggcaatca tcaaaatcat
    17521 cattaaaatt ttaaattcaa atattgatgg taaaagtttt ggaaatctgt gcaaacagta
    17581 aaattcatct atatcaaata agcatcaagt agttagatgt aatagtggaa tgtaacttta
    17641 tttcagaata caatacaggt attgaaattt cgtttcacag cttggagtat atacatgagc
    17701 attaaagtag cgattaacgg tttcggccgc atcggccgcc tcgcattgcg tcaaattgaa
    17761 aaagcccacg acatcgaagt cgtcgccgtc aacgacctga cccctgccga aatgctgctg
    17821 caccttttca aatacgacag cacgcaaggc cgtttccaag gtactgccga attaaaagac
    17881 gatgccatcg tggtaaacgg caaagaaatc aaagttttcg ccaatccgaa tcccgaagaa
    17941 ttgccttggg gcgagttggg tgtggatgtc gtcctcgaat gtaccggctt ctttaccaat
    18001 aaaaccaaag ccgaagccca catccgtgcc ggcgcgcgca aagtcgtgat ttccgcacct
    18061 agcggcaatg atgtgaaaac cgtcgtatat ggcgtaaacc aagacatttt ggacggcagc
    18121 gaaaccgtta tctccgctgc ttcctgcacc accaactgcc ttgccccgat ggcggcagtc
    18181 ctgcaaaagg aattcggcgt agtcgaaggc ctgatgacca ccatccacgc ctacaccggc
    18241 gaccaaaaca cccttgacgc gccgcaccgc aaaggcgatt tgcgccgcgc ccgcgccgcc
    18301 gcgctcaaca tcgtgcccaa cagcaccggt gccgccaagg ccatcggtct ggttatccct
    18361 gaattgaacg gcaaactcga cggctccgcc caacgcgtcc ctgttgccac cggctcgctg
    18421 accgaattgg tttccgtcct cgaacgccct gtaaccaaag aagaaatcaa cgccgcgatg
    18481 aaagccgccg ccagcgaatc ttacggctac aacgaagatc aaattgtttc ttccgacgtt
    18541 gtcggcatcg aatacggttc gctcttcgat gcgacccaaa cccgcgtgat gaccgtgggc
    18601 ggcaaacaat tggtgaaaac tgttgcctgg tacgacaacg aaatgtccta cacctgccaa
    18661 ctcgtccgca ctttggaata cttcgcaggc aaaatctaag gcttgaacaa acctgtggat
    18721 aagtaaatgc cgtctgaaca cattgcgttc agacggcatt gtttatctgt atagtggatt
    18781 aaatttaaac cagtacggcg ttaccttgcc ttgccgtact atttgtactg tctgcggctt
    18841 cgttgccttg tcctgattta aattcaatcc gctataactt gatattgcgg cttatggcga
    18901 acaaacaaca gccgtttgtt caactgctat gcggcaaaac cggatttttg cccgatttaa
    18961 agcggggttg gggaaagatc aataacggtt gcacggtttt cctcgatttg aaatctgatt
    19021 tcgtaatccg caatattcat cacataaatc cgttcgggaa tattctgata ggcggggcgc
    19081 ggatcttggg caatgctttg gctgataagg tttttggtgt ttgcagataa attttccgcg
    19141 ccgatgtttt cctgccaaac gacttccaac tctacgggtt tgccgctgac gaaaccagat
    19201 gcggcatcgg gtttggattc gacaaagggg atataaggct tgatgtccac aatcggcgtg
    19261 ccgtcgagca ggtctgcgcc gctgcaataa aggcggacgg gtttgccggt ttcgatgcgg
    19321 ttgagtttta agagtgatag tccgagatgg ttggggcggt gggggctgcg cgtggcgaat
    19381 acgcccattt tttgtttgcc gccgagccgt ggtgggcgca ccatttgtgc ccagccttca
    19441 tccaatacgc cgtgaaaaat aaaacttatc cacacataat cgaaatcttc cagcccgcgc
    19501 acgctgtctg cggtaaattc gggattcagc tcgatgcata cttctgcggc ggagactaaa
    19561 ccgggctggc gggcgatgcc gaatttctgt ttgtagggcg atcgggcggt gccgatgggg
    19621 gtaatggtat aagtcataag gttgtggata agtttttttg gaaaaacctt atcttatcac
    19681 aatcggtttt accgtttttg ggcagtaatt tgttataata cagggcattt taatcgcgtt
    19741 gaaataatga gatgattgtt tccattgatg ttgatgcaca aaaaacgttt acgccactgt
    19801 gtcctgacga actgcccgtg aacgaggggc atttgattgt cgaggagttg aatgctcaag
    19861 ccgctttggc ggatttgcgc gtgatgacga aagatgcgca tcatatggta gcgaaatggc
    19921 ttgtggataa ccctgttgat atgttgaagc cgacaggttt gcctgatgcg gatttgactt
    19981 gggtggctca tgcgatggtc ggtacgcgcg gctatgaatt attggacgga ctgccttctg
    20041 ccaaagaata tgattattgc gtttggaaag gtgttgatcc tgaattgcat ccttacggcg
    20101 cgtgttttca cgatattgag gaaaaactaa gcacagggct gattgaatgg ctgcgttgtc
    20161 aaaatacgga tacggttatt atcggtgggt tagctacgga ttattgtgtt aaaacaacgg
    20221 ttttgcagtt acttaaaggt ggtaattggc aggttatcgt caatgaagcg gcttgtcggg
    20281 gcattgcgcc ggataccatt gaaacggcat ggcaggaaat gagttctgcc ggtgcagtca
    20341 tcttaaaaaa cgctgaagaa attaaaaaat atattaataa tcaataagtt aaaattgttt
    20401 cacgtgaaac atacttctta aaatatgaaa attttgcttg tccgtttgtc tagtatgggc
    20461 gatttaattc acactttgcc cgcaattgag gatttggcgc gacaatgccc tgatgtagaa
    20521 ctccattggc tgtgtgaagc tggatttaca gatattgcgc gcctgcatcc gtttgtgaaa
    20581 aaaatccatg tgatgaaatg gcggcaatgg cgcaaacatc tctttcaggc tgaaacttgg
    20641 cgggaaatgg gtcatctgaa acagactttg cggcaggaag catttaattt tgtattggac
    20701 agtcaaggtc tgattaaaag cgcgtgtttc gctaaaatgg cgaaatcccc tatttatggt
    20761 ttggataaaa atagcgcgcg cgagggactg gctgcgctgg cgtatgcgaa aacatacgct
    20821 gtaccgaaag ggaaaaatgc tgtttggcgc aatcgtgagc tgttcgccca agtatttggg
    20881 tatgcgatgc ctgaaacgca ggtatttggc ttgactgttc ctgaggtagg ccgtctgaaa
    20941 aatttagcgc agccgtatta tgcggctttg catgcgacca gtcgggacag taagttatgg
    21001 cctatggaaa actggcggtc gttgctgcaa aagttgaatg aagaacagca atgcaatatt
    21061 tacctgcctt ggggaaatga agatgaaaaa acgcgtgcca aacagattgc agacggactg
    21121 ccgtttgcta ttgtgtgcga caaaataaat ttattgcagg cagcgtatct gctgaaatac
    21181 gcggtcggaa ttgtcggcgt ggataccggt ttgctgcatt tggcaaatgc cttggaaaaa
    21241 cctgtggtcg gcatttatac cgataccgat ccgattaaaa caggcgttca ggtttcgcca
    21301 attgcaaaaa atttgggcaa tatcgggcag attccgaccg cagatttggt ttatcaaacg
    21361 ttgatggatt gtgttgcagc agacaaaggc taaagggttg tctgaaactg atatagcacc
    21421 tgatattttt cagtttgtta agcataagaa tgtatatcta ccagcctgag taaatgcttt
    21481 aaaaatggta taatcggcaa gcattatccg aaagattttt gaatgaattc gtttttaagg
    21541 aaaacggatt tggttttata gggaagaagg tttggtttcg ctggatggtg aaagcagcgt
    21601 tctttttagg agggttcaat gaaagcactg gtcgcagtaa agcgcgtagt ggactacaac
    21661 gtcaaagttc gtgtaaaagc cgatggttcg gatgtggata tcggcaatgt caaaatgtcg
    21721 atgaatccgt ttgacgaaat cgctgtggaa gaagctgtcc gtttgaaaga agccggaaaa
    21781 gtaagcgaaa tcgtagcggt ttctttgggt gagaaaaaat gcgaagaaac cttgcgtaca
    21841 gctttggcga tgggtgccga ccgtgccatt catgttgaaa ccgatactaa actggagtct
    21901 ctggccgttg ctaagttgct gaaagccgtt gcggacaaag aaaatccgca aattttcttt
    21961 ttgggcaaac aagcgattga tgatgatgcc aatcaagtgg cgcaaatgct ggcagctttg
    22021 ctgaatgcgg cgcaaggtac gtttgcttca aaagtacaca ttgaatgtgg tgaagtacag
    22081 attgtgcgcg aaattgatgg cggcgaagaa accatagcat taaaactgcc tgctgttatc
    22141 agtgctgatt tgcgtttgaa cgagccgcgc tttgtcaaac tccccaatat tatggcggca
    22201 aagaaaaaac ctttggaaaa actgactcct gacgatttgg ttgccgacat ttcacctcgt
    22261 ttgaaaacgg tgaaatttgc cgaacctaag gcgcgtcagg caggcgtaaa agtagcaagc
    22321 gttgccgaat tggttgaaaa attgaaaaac gaagccaaag tgatttgagg aggctgaaat
    22381 gagtgtattg attattgtcg aacacgacaa caaacagttg aatcctacca ctttgcatgc
    22441 tgttaccgct gccgctaagc tgggtaaaat cgatttattg gttgccggaa gcggtgcatc
    22501 tgctgtggta gagttcgcaa aacaagtggc gggtgtggaa aaagttttgg ttgcagatgc
    22561 tgcccattat gccgaaggtt tggctgaaga gttggctccg ctggttgtta aattggcagt
    22621 ggattactgc tatgttgcag caacggcaac tacattcggt aaaaaccttt tgcctcgcgt
    22681 agcagcctta ttagacgtac cgcaaatttc tgatttgacc gaaattgtgg ataacacgac
    22741 ttttgtgcgc cccatttatg cgggtaatgc atttgaaacc gtgcaagctg attcagaaaa
    22801 attggtgctg accttccgtg cgacggtttt tgacgcagtg gcggcgcaag gtggtaatgc
    22861 tgaggtaatt aatgttgaag caacccctgc ccaaaacctg agtcgttttg ttaaccgtca
    22921 gctttcccat tccgatcgtc ctgaactgac tcaggcaaaa gtgattgttt ccggtggtcg
    22981 tgcgttgggt agtgcggaaa aattcaatga agtgctgaca ccgttggcgg atgttttagg
    23041 tgcggcaatc ggtgcatccc gtgcagcagt tgatgccgaa tatgcgccaa acgatgctca
    23101 agtcggacaa accggtaaag tggttgcgcc gcaactctat tttgcaatcg gtatttcagg
    23161 tgcaattcaa cacgttgccg gtatgcaaga cagtaaggtg attgttgcaa tcaataaaga
    23221 tgctgatgcg ccgattttca atgtagccga ttacggattg gttggcgatt tatttgaaat
    23281 cgttccacaa ttaacagaag tattgaaaaa ttgatgtttc acgtgaaact ttcagacgac
    23341 cttgttatta atgaggtcgt ctgaaaacat ttcagcatga ttacgactta taaaaccatt
    23401 acttcctcga cgcaggctga gtttaaagat aaaggcagcc gttttattgc atttacctat
    23461 ccgattcgga cattggctga tgtgaaaaaa tacctcgatc tgttaaagga agagcatcat
    23521 aaagcacgac actggtgcta tgcctatcgt ttgggtgtgg atggtgtgca atttcgtgcc
    23581 aacgatgatg gagagccatc aggaagtgct ggacgaccaa ttttgggaca gattgattcg
    23641 gtgggtatta ccgacgtttt ggttgtggtc gtccgctatt tcggtggtac tttgttaggt
    23701 gttccaggtc tgatacatgc gtacaaagag gcgacggctc aagcgttggc agttgcagaa
    23761 gtagttgaaa agaatattga aaaaactgtt tggctgaaat gtgaatatcc gtttttgaat
    23821 gaagcgatac gcattgccaa acaatatcag gcggatatat tggagcggga tttgcagttg
    23881 gattgccgat tgacggtcag tttgtcgttg gtaaattatg aggcctgtgt tgcggcttgg
    23941 aagaatactc ggcagattga agtaaatacg gaaaagcctt ttgaatgaaa ggtcgtctga
    24001 aatgctttgt gatggtattt cagacgacct cttcgctgac taatcttctt gtgtatattt
    24061 gtctaaggca tcgacgctgg agcgcaaata taaccaagcc aattgatgga attcgcccag
    24121 cgcgttccat agcgcatctt cactcataat gctgtattcg ccttccgtac tcaaatccag
    24181 ttctaccccg tcttcaatag cctgatgaca cgcctgatac tcgtgggtat agaaattgtc
    24241 catgtcgcga attaaagtga cgacatattt ccaacggcgg atgtcttctt ccagttgcgg
    24301 tagtaaatga caccattcgc ggtatttgct ttggatttca tcaatacgtt tttgcatggt
    24361 tgcttgtcct tttttaattt atggataagt tgaataggtt gcagagtgga atgaagcggt
    24421 cttttgtttg attctactaa aatttcttca gatgggataa aagtcccttt gtatcgagtt
    24481 ttcgttaaaa gtacatattt gtcaaggaga tggtatgaaa ctgctggtta tcggtaatgg
    24541 cggtcgcgaa cacgcgctgg cttggaaatt ggcgcagtcg cctaaagtgg aaacagtatt
    24601 tgttgcgcct ggtaatgccg gtacagcgat tgaacccaag ttgcaaaaca tcgacttgac
    24661 tgcgcatcag gatttgattg aattctgtcg taaagaaaat attgttttta ccgtcgtcgg
    24721 ccctgaagcg cctttggcgg caggtattgt ggatgatttc cgtgctgcag gactaaaaat
    24781 attcggtccg acacaatatg cggcgcagtt ggaaagttcc aaagatttcg ccaaagcatt
    24841 tatggcgaaa tacaatattc cgaccgcgca atatcaaact tttgaaaacg ccgatgccgc
    24901 acatgattac gtcaatcaga aaggtgcgcc tatcgttatc aaagccgatg gtttggcggc
    24961 aggtaaaggc gtgattgtgg cgatgacttt ggatgaagcg catgctgcga ttgatgatat
    25021 gctgctggac aacaaaatgg gcaatgccgg cgcgcgtgtt gtaattgaag atttcctgca
    25081 aggcgaagaa gcgagtttta tcgtcatggt tgatggcaat aatgtactgc ctatggcaac
    25141 cagccaagac cacaagcgtc ttttagacgg cgacaaaggt ctgaatacgg gcggtatggg
    25201 tgcgtacagc cctgcgcctg tggtaacgcc tgttgtgtac gaacgcgcga tgaatgaaat
    25261 tattttgccg accgtagcag ggatgaaagc ggaggggcat gagttcaccg gtttcctgta
    25321 cgcaggtttg atgattgatc aaagcggtgc accctatacg attgagttta actgccgttt
    25381 cggcgatcct gaaacccagc cgattatgag ccgactgaac agcgacttat cggatttggt
    25441 tgaagcggca atagacggca aactcgatag cgtaaccgcg gaatggagcc cgcaaactgc
    25501 cgtgggcgtg gtactggcgg cgcaaaatta ccccgaaacg cccaaaaaag gcgatattat
    25561 ttccggtctg gatgctgcca accaaatcgg caaagttttc catgcaggta caacggcaaa
    25621 agagaaaggt gacgtattga ccaacggcgg acgcgtattg tgtgttgtgg gattgggcga
    25681 caatgtggcg caagcgaaag ctaaggcata tggcgcgttg gaaaaaatca gtttcgacgg
    25741 gatgcaatac cgtaaagaca ttgccgataa agcgattaac cgttaactag aaagaggatg
    25801 atgccgtctg aagccaagta tgactgatgt gcttcagacg gtattttata ttatgttatt
    25861 tccgagaatt atcgcagcat cagcgcaacg taagttaagc gtttatttga aaaaaggcgg
    25921 tttggtcgct tatccgaccg aatcttgtta tggcttgggg tgtcttccga cattagccaa
    25981 agcactcggt aagttggcgc atttgaaaaa aagaccgcaa cacaagggta tgattgtcat
    26041 cgggaatcaa tttgagcaat tgcagccttt acttcaaatg ccgtctgaaa atctgcagga
    26101 tatgcttaga aaagaatggc ccgcccctaa aacgttccta ttatccgcca aatcctgtgt
    26161 cttacctgag ttgcgcggga aacagcgcag caagttggcg gtcagggttc ccgcccatgt
    26221 aggtgcgcgc cgcttgtgtc aggctttgca aacgcctttg gtctcgactt cgtgcaaccg
    26281 cgcaggcaaa cgcgcgtgtc ggacggagag ggaagtcaga aggcaattcg gacgcgatgt
    26341 gtggattgtt ggtgggagaa tcggcaggca gaaatcgccg agtcagatta ttgacgggga
    26401 aacaggtaaa cgtttgcgtt agatatgggt atttgaatga aaactttttt tgcatacaca
    26461 aagaaaaaat tttggggctg tactagatta gccctaaatc ccacaccaat cccgcaggat
    26521 tttaagctgt tgagacggtg tgccgaagtt aaatcgaaat tcgc
//
LOCUS       DAWKST010000005        26524 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.005, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000005 DAWKST010000000
VERSION     DAWKST010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26524
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.005"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            123..2150
                     /gene="uvrB"
                     /locus_tag="WJL87_000133"
     CDS             123..2150
                     /gene="uvrB"
                     /locus_tag="WJL87_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ7798628.1"
                     /translation="MEVIRYPDSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IDFFTKENRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSSKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDGGKGRRQGKNKVKV
                     SEIHNEEDAVKEIAKLEKAMQQAARDLQFEEAAVLRDRIRGIKEGLLFGSE"
     gene            2262..2549
                     /locus_tag="WJL87_000134"
     CDS             2262..2549
                     /locus_tag="WJL87_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798629.1"
                     /translation="MTYSFRVENINDISDIPENHLEIVRAALPSNRSNALSREPDGAQ
                     AVFVYEIDGEFRAKLFDRIKDTDSYLQATEDNYSNDPRSIGVVYVLEQQQG"
     gene            complement(2730..3446)
                     /gene="trmB"
                     /locus_tag="WJL87_000135"
     CDS             complement(2730..3446)
                     /gene="trmB"
                     /locus_tag="WJL87_000135"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ7798630.1"
                     /translation="MTDTAENQTQNNWQAEHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDANFGSNRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLINENHLENIRVMRHDAVEVIENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYARQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRMG"
     gene            complement(3568..4632)
                     /locus_tag="WJL87_000136"
     CDS             complement(3568..4632)
                     /locus_tag="WJL87_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ7798631.1"
                     /translation="MYLLYASRHFEEALEWAKTSAKNNNPHGQYLLAQYCRYGTPPDF
                     ETAHLLYRKAAAQGLPEAHWQLGLQYRFGQGTKVDTAQAVNHLRAAAQQGYIPAYTPL
                     AELILPTAPDEAVYRFQQAAQENDPDSHAALADIYLQGKYQERNHKLALHHAEAAAAE
                     RHPEGLRILGDICRYGLGIAPDTEKARHYYRQAAEAGSLSAYQKLISDSALNHPDQYG
                     GIKDSAIRRQRAEQLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMY
                     YFGQGIAADYGKARKWFEQAAAQKDSMAFYNLACIHYSGHGVGPDKEKACRYLQEAIN
                     NGYGQKSVLQELLQQWQNAV"
     gene            complement(4697..4972)
                     /locus_tag="WJL87_000137"
     CDS             complement(4697..4972)
                     /locus_tag="WJL87_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798632.1"
                     /translation="MNNPDLPYRQTLECLSQKQYNFTEVRRLLTEAALAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGAPP"
     gene            complement(5089..6975)
                     /gene="uvrC"
                     /locus_tag="WJL87_000138"
     CDS             complement(5089..6975)
                     /gene="uvrC"
                     /locus_tag="WJL87_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ7798633.1"
                     /translation="MPSEAVFQMIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVRHIETTITRSESEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGIVCVHWVSIRGGRHVGDKSFFPDTKNDPDPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFSVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSSQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPHSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            complement(7030..9192)
                     /locus_tag="WJL87_000139"
     CDS             complement(7030..9192)
                     /locus_tag="WJL87_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEZ7798634.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VTFDDSKTSAADIAKIIEKTGYGAKEKTEDTLPQAEAEHHIGWRLWLLSAINIPFLVG
                     MAGMMIGRHDWMIPPLWQFALASIVQLWLAIPFYKSAWASIKGGLANMDVLVTIGTVS
                     IYLYSVYMLFFSPHAAHGMAHVYFEAGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKL
                     TPTQVNVQRDGEWKQLPIDQVQIGDLIRANHGERIAADGVIESGSGWADESHLTGESN
                     PEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIARVADKA
                     AAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAIMVGMG
                     KAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGRPQVAAVYCVPDSGFDEDDLYRI
                     AAAVEQNAAHPLARAIVSATQARGLEIPAAQNAQTVVGAGIAAEVEGAGLVKAGKAEF
                     AELTLPEFSDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNIDVYIM
                     SGDNQGTVEYVAQQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDAPALAA
                     ANVSFAMKGGADVAEHTASATLMQHSVNQLADALLVSQATLKNIKQNLFFAFFYNILG
                     IPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            9331..10281
                     /gene="trxB"
                     /locus_tag="WJL87_000140"
     CDS             9331..10281
                     /gene="trxB"
                     /locus_tag="WJL87_000140"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ7798635.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            10436..10804
                     /gene="rpsF"
                     /locus_tag="WJL87_000141"
     CDS             10436..10804
                     /gene="rpsF"
                     /locus_tag="WJL87_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ7798636.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            10805..11107
                     /gene="priB"
                     /locus_tag="WJL87_000142"
     CDS             10805..11107
                     /gene="priB"
                     /locus_tag="WJL87_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ7798637.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            11114..11344
                     /locus_tag="WJL87_000143"
     CDS             11114..11344
                     /locus_tag="WJL87_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ7798638.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            11361..11813
                     /locus_tag="WJL87_000144"
     CDS             11361..11813
                     /locus_tag="WJL87_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ7798639.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEVRRAELEAKQAEILADARVRQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            11827..11934
                     /locus_tag="WJL87_000145"
     CDS             11827..11934
                     /locus_tag="WJL87_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HEZ7798640.1"
                     /translation="MPSETLPIVGMWFQTVFVLFSVYSAKVGMVVFIVD"
     gene            complement(12601..13407)
                     /locus_tag="WJL87_000146"
     CDS             complement(12601..13407)
                     /locus_tag="WJL87_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ7798641.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKRAVDWKTVFAMMPGMIFGVFTGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTGRQTASRPLPKLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(13408..14154)
                     /gene="pssA"
                     /locus_tag="WJL87_000147"
     CDS             complement(13408..14154)
                     /gene="pssA"
                     /locus_tag="WJL87_000147"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ7798642.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            complement(14209..14631)
                     /locus_tag="WJL87_000148"
                     /pseudo
     CDS             complement(14209..14631)
                     /locus_tag="WJL87_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14737..15219
                     /locus_tag="WJL87_000149"
     CDS             14737..15219
                     /locus_tag="WJL87_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEZ7798643.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGAMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGVQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(15271..16482)
                     /locus_tag="WJL87_000150"
     CDS             complement(15271..16482)
                     /locus_tag="WJL87_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ7798644.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFTAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            16705..19143
                     /locus_tag="WJL87_000151"
     CDS             16705..19143
                     /locus_tag="WJL87_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ7798645.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVMLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNSKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPSPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDSPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPTD
                     LNGSRKILAHKDHL"
     gene            19352..20662
                     /locus_tag="WJL87_000152"
     CDS             19352..20662
                     /locus_tag="WJL87_000152"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ7798646.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFHDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NSDRVIIDFEGKIDGEPFAGGASKNYAFVLGAGQMLPEFEAGVVGMKAGESKDVTVNF
                     PEEYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVQKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDIFKEQAERRVSLGLILAKLVDENKLEPTEEQVKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQAPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            20758..21372
                     /gene="clpP"
                     /locus_tag="WJL87_000153"
     CDS             20758..21372
                     /gene="clpP"
                     /locus_tag="WJL87_000153"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ7798647.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLQF"
     gene            complement(21429..22694)
                     /gene="ispG"
                     /locus_tag="WJL87_000154"
                     /gene_synonym="gcpE"
     CDS             complement(21429..22694)
                     /gene="ispG"
                     /locus_tag="WJL87_000154"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ7798648.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKITLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(22710..23471)
                     /gene="pilW"
                     /locus_tag="WJL87_000155"
     CDS             complement(22710..23471)
                     /gene="pilW"
                     /locus_tag="WJL87_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ7798649.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLIGQ"
     gene            complement(23474..24568)
                     /gene="rlmN"
                     /locus_tag="WJL87_000156"
     CDS             complement(23474..24568)
                     /gene="rlmN"
                     /locus_tag="WJL87_000156"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ7798650.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFNE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVKDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(24711..25136)
                     /gene="ndk"
                     /locus_tag="WJL87_000157"
     CDS             complement(24711..25136)
                     /gene="ndk"
                     /locus_tag="WJL87_000157"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ7798651.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            complement(25195..26346)
                     /locus_tag="WJL87_000158"
     CDS             complement(25195..26346)
                     /locus_tag="WJL87_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ7798652.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
BASE COUNT         6298 a         6630 c         7220 g         6376 t
ORIGIN      
        1 tgggtagcga ttgggtttca gacggcatac attcaggatt tcaccgcctt atgccgtctg
       61 aaagacgttt ccgacccatc cctactacac gctttgtccg caaacgcatc acaggcaaga
      121 ttatggaagt catccgatac cccgattccc ctttcaaact ccaccaaccc ttcccgcccg
      181 caggcgacca gcctaccgct attgccggct tgctcgaagg gctttcagac ggccttgcct
      241 accaaaccct gctcggcgta accggttcgg gcaaaaccta caccatggcg aacgtcatcg
      301 cccaaagcgg ccgacccgcc atcatcatgg cgcacaacaa aacccttgcc gcccagcttt
      361 atgccgaaat gcgcgagttt ttccctgaaa acgcggtgga atatttcgtc tcctactacg
      421 actattacca acccgaagcc tatgtgccca gccgcgacct gttcattgaa aaagacagcg
      481 cgatcaacga acatatcgag cagatgcgcc tttccgccac caagaacctg atgacgcgcg
      541 acgacgtgat tatcgtcgcc accgtgtccg ccatctacgg tatcggcgac ccgaccgagt
      601 atcagcaaat ggtgttgtcc gttaaagaag gcgacaccat tgagcagcgc gacatcatcg
      661 ccacgctcgt ttccatgcag tacgagcgcg gcgatttgga ctttaaacgc ggcagcttcc
      721 gcgtgcgtgg cgacgtgatt gacgtgtacc ccgccgaaag ctccgaaaac gccttgcgca
      781 tcagcctgtt tgacgacgaa atcgaccgcc tcgatatgtt cgacccgctt tcaggcagcc
      841 ttatccagcg cgtcggccgc tacaccgtct tcccgtccag ccattacgtt accccgcgcg
      901 ataccgtctt gcgcgcctgc gagtccatca aagaagaatt acgtgaacgc attgatttct
      961 ttaccaaaga aaaccgtccc gtcgaacaac aacgcatcga acagcgcacc cgctttgacc
     1021 tcgaaatgct ctacgaaatg ggcttctgca aaggcatcga aaattactcc cggcacttct
     1081 ccagcaaaaa agaaggcgaa ccgccgccca cgctgatgga ctacctgcct gacaacgcca
     1141 tcatgttcat cgacgaaagc cacgttaccg tcacccaaat cggcggcatg tacaaaggcg
     1201 acgcatcgcg caagcaaaac ctcgtcgact acggcttccg cctgccttcc gcccgcgaca
     1261 accgcccgct caaattccat gaatttgaaa aagtcatgcc gcaaactgtc ttcgtttccg
     1321 ccactccagc caaatacgaa gaagaacacg ccggacaagt ggtcgaacaa gtcgtccgcc
     1381 ccacagggct ggtcgacccc caaatcatca tccgccccgt cgccacccaa gtcgacgact
     1441 taatgagcga aatcaacgac cgcatccaaa aaggcgaacg cgttctcgtt accaccctca
     1501 ccaaacgcat ggcggagcaa ctcaccgact attacagcga actcggcatc aaagtgcgct
     1561 acctgcacag cgacattgat accgtagagc gcgttgaaat cattagagat ttacggctcg
     1621 gcctgtttga cgtactcgtc ggcatcaacc tcttgcgcga aggcctcgat atccccgaag
     1681 tctccctcgt cgccatcctc gacgccgaca aagaaggctt cctgcgctca caccgcagcc
     1741 tgattcaaac cataggccgc gccgcgcgca acgtgaacgg cgtcgcgatt ctgtacgccg
     1801 acaaaatcac cgactctatg aaagccgcca tcgacgaaac cgaacgccgc cgtgaaaaac
     1861 aaattaaatt caacgaagaa cacggcatcg tgccgcaaca gattaaaaaa caggtcaaag
     1921 acatcattga cggcgtgtac cacgaagaag acggtggtaa aggccgtaga caaggcaaaa
     1981 acaaggttaa agtcagcgaa attcacaacg aagaagacgc ggttaaagaa atcgccaaac
     2041 tggaaaaagc catgcagcag gcagctaggg atttgcagtt tgaagaggcg gctgtactga
     2101 gggataggat acgggggatt aaggaagggt tgttgtttgg gagtgagtaa taaaagcact
     2161 tgaaagttta gttgatgacc acaatgttct taaaagatct ggagaaacaa aagatgtgga
     2221 gaaacatcgt aacttgatca attacattct gaggactgaa aatgacatat tcttttcgtg
     2281 tagaaaatat aaatgatata tctgatattc ctgaaaatca tttggaaata gttagagctg
     2341 ctctgcctag taatagaagt aatgccttat ctcgtgagcc agatggggca caagctgttt
     2401 ttgtttatga aattgatggt gaatttagag ctaagttatt tgaccgcata aaagatacag
     2461 acagttattt acaagcaaca gaggataatt actctaatga ccctcgcagt ataggagtag
     2521 tatacgtttt ggagcaacag caagggtagc ctgagggccg cacaaactct caagctgacg
     2581 tgcagtatgt gtgatacgca cgcatgcgtt ttttcgttca aaccattgtt ttaaaccttg
     2641 tatcgaaaca tcactcgctg ctttatcttg aaaaaatgcc gtctgtaagc aaacatgctt
     2701 caaacagcat ttttttatcc ggcggtttgt tagcccatcc gtttgaatac caagtcccaa
     2761 acgccgtgcc cgaggcgttt gccgcgcgct tcgaatttgg tttcggggcg gtagtccggg
     2821 gtgggggcgt agtctgccgc cgtattttgc aggctgtcga agctactgag gacttcaagc
     2881 atctgccgtg catattcttc ccagtctgtc gccaggtgga tatagccgcc ggttttgagt
     2941 ttgggcagca gtttggcgat gaagggggct tgtatcagac ggcgtttgtg gtggcgtttt
     3001 ttgtgccacg ggtcggggaa gaatatgtgg atgccgtcga gcgagccgtc ttgcagcata
     3061 ttttcgataa cttctacggc atcgtgccgc atcacgcgga tgttttccaa atggttttcg
     3121 ttgatgagtt tgagcagatt gcctacgccg ggaccgtgta cgtcgatggc gaggaagtcg
     3181 gtttcgggca ggcggcgggc gatttctgcg gttgccgtcc ccataccgaa gcctatttcg
     3241 aggattttgg gtcggttgct tccgaaattg gcatcaagat cggccggtgt tgcttggtaa
     3301 tcgatgccga agctgtccca taaggtatcg atggcgcgtt gctgtgcggc ggtcatatgg
     3361 ctttggcgga ggacgaagct gcggatgctg cggggatgtt cggcttgcca gttgttttgt
     3421 gtttggtttt cggcggtgtc tgtcataatg cttccgtgcg cttgtgcaat caggatgccg
     3481 tctgaatgtg aaaggcctgt tcggacggcg ggaagggaaa ggggcgatta tacctgtttc
     3541 gtttcggcag ggtaggtgta acgctgttca gacggcattt tgccattgtt gcagcagttc
     3601 ttgcaggacg cttttttgcc cgtatccgtt gtttatggct tcttgcaggt agcggcaggc
     3661 tttttctttg tccggcccga caccgtgtcc gctgtaatgg atgcaggcga ggttgtagaa
     3721 cgccatactg tctttttgcg cggcggcttg ttcaaaccat ttgcgggctt tgccgtagtc
     3781 ggcggctatg ccctgtccga agtagtacat actgcccaga ttggtttggg ctttgctgtg
     3841 tccgagttct gcggcttctg tgtagagttt gagcgcggct gcgtattcgg gcgcacattg
     3901 taatccgtaa tgcagggctt gggctttttg ataaagctgt tctgcccttt gccgcctgat
     3961 ggcggaatct ttgatgccgc cgtattggtc gggatggttt aacgcgctgt cggatatgag
     4021 tttctgatag gcggaaaggc tgccggcttc ggctgcctgc cggtaataat gccgggcttt
     4081 ttccgtatcg ggggctatgc ccaaaccgta gcggcagatg tcgcccagta tccgcaaacc
     4141 ttcgggatgg cgttctgctg cggctgcttc ggcatgatgc agggcaagtt tgtggtttct
     4201 ttcctgatat ttgccttgta ggtagatgtc ggccagtgcg gcatgggaat cggggtcatt
     4261 ttcctgtgcc gcctgttgga accggtaaac ggcttcatcg ggagccgtag gtaggatgag
     4321 ttcggcaagc ggggtgtagg cgggaatgta tccttgttgc gcggcggcgc gcaaatgatt
     4381 gacggcctgt gccgtgtcga ctttcgtccc ttgcccgaaa cggtattgca gcccgagctg
     4441 ccaatgtgct tccggcaagc cttgtgccgc cgcttttcgg tagagcaggt gcgccgtttc
     4501 aaaatccggc ggtgtgccgt accggcagta ttgggcgagc agatattgtc cgtgggggtt
     4561 gttgtttttt gcgctggttt ttgcccattc gagtgcttct tcaaaatgcc gggacgcgta
     4621 cagcaggtac atcaggcgga cttgcgcttc gggcactcct gcttctgccg gaggcctgta
     4681 aagcgatatc agggtttcag gggggcgcgc cttccaattc ttggatatat gccagattgt
     4741 aacgcgcgta gggatgcccc tgttcggcgg cgatgcggag catttccata ccttgttcgc
     4801 ggtcttggta ggggctgtcc gcatccatca ggtgttttgc caactcgaat gcggcggcgg
     4861 gatgacctgc caaggccgct tctgtcagca gtcggcggac ttcggtaaag ttatattgtt
     4921 tttgagaaag gcattctaag gtctgccgat agggtaagtc gggattgttc atatcagatc
     4981 gttttccgtt ttgtcgaatc atcgttatat gttatgaagt taggtttata tcggcacgcg
     5041 ggcctgctgt atcatatttt tacagacggt ttttgttttg gcggcatact aatgcagatg
     5101 attataaatg gtttccgcca atgccttgct gatgccttcc actttttcca agtcctcgcg
     5161 gctggcggca atcacgccgc gcaggccgcc gaagcgggtg agtagggctt ggcggcgttt
     5221 gctgcccacg ccggggatgt cgctgaggga cgaggtaacg cgtgctttgt cgcgtttttt
     5281 acggtggcct gtaatggcaa agcggtgtga ttcgtcgcgc acggtttgca gaaggtgcag
     5341 ggcggggctg tgcgggggca ggcggaagac ttcgccggta aaaggcagta tgagctcctc
     5401 cataccggct ttgcgctccg ggcctttggc gataccgacc aaaggaatgt gcagcccgag
     5461 ttcttcccat accgatacgg ctacgccgat ttgccctttg ccgccgtcaa ttaacacggc
     5521 atccggccat ttgacggtct cgccgttggc ttcggcctcc tgcattttgc cgtaacggcg
     5581 cgtcaacact tcgcgcatag cggcgtagtc gtcgccgggt ttggcggtcg tgatgttgta
     5641 gcggcggtat tgcgaaggct ggatgttttg ctcatcgtac acaacgcagg acgcaatagt
     5701 ggcttcgcct tgtgtgtggc tgatgtcgaa acattcgagg cggttgaggc cgtctgaatc
     5761 cataccgagg attttggcga gttcgtcgat gcggtgttgc tggctgcttt gttgcaggcg
     5821 gcgttgtgca atcgccattt gtgcgttttg ttccgccatt ttcaaccaga ctttgcgttc
     5881 gcctatggtt ttggtgacaa attgcatctg cttgccgtgt tcgccttcca aagcctcttt
     5941 cagcgcatcg ggaacggaaa agttgctaat aatgatgtcg ggtttgcttt tgcccaaata
     6001 atgttgggcg acgaaggctt cggcgtaatc ttgtccgttt ggatcgggat cgtttttggt
     6061 gtcggggaaa aagcttttgt cgccgacgtg ccgcccaccg cgtatgctga cccagtgtac
     6121 gcagacgatg ccgtctgaaa ccgccagcgc gagcaaatcg atatcgttgg ggttgttcgg
     6181 gtttttgctg tcgatgaact ggttgctctg cataatgccg agtgcttgga tttggtcgcg
     6241 gtagcgggcg gcttcttcaa attgcagatt cgcggcggca gtttgcattt tgtgttgcag
     6301 ggtgcgggtc agttcgtcgg ttttgccgtt gagaaaagtg gcggcttgac gcacgctgtc
     6361 gcggtagtct tcttcgctga tgtgtccgac gcagggcgcg gtacagcgtt tgatttggta
     6421 cagcaggcaa gggcggtcgc ggtgttcgaa cacgctgtct tcgcaggtac gcagcataaa
     6481 gactttttgc aggacttgga tgctgtcgcg cacggcgttg ctgttgggat acgggccgaa
     6541 gtattggttg ggctttttca gcgtgccgcg gtaatacgcc atttgcggat attgatggcc
     6601 gctgagcatc agataaggat agcttttgtc gtcgcggaaa agaatattgt atttgggcga
     6661 cagggctttg atgaagttgt tttcgagaat cagggcttcg gattcggagc gggtgatggt
     6721 ggtttcgatg tggcgaacct gttttaccat caatgcgatg cgcggcgaat ggtcgttttt
     6781 ttggaaatag ccggacacgc gccgcttgag gttgaccgct ttgccgacgt ataagacttt
     6841 gccgccttcg tcaaaaaaac ggtacacgcc cggcagcttg ggcaggtttt tgaggaagag
     6901 cgggatgtcg aattgttccg gagaacggtt ttctgtattc atatcaatca tttgaaaaac
     6961 ggcttcagac ggcatatcgg cagcggcagc cttatccgtt cggaaaagct gtcgggtggt
     7021 tatatgcggt caatcgattt ttacccgttt caggcgcaag gcattgctca acacggaaac
     7081 cgagcttgcc gccatggccg cgcctgcgat gacgggattt aaaaagccaa gcgcggcgag
     7141 cggaatgccc aagatattgt agaagaaggc gaaaaacagg ttttgcttga tgtttttcaa
     7201 agtggcttgc gacaccagca gggcatcggc gagctgatta accgaatgct gcatcagcgt
     7261 ggcggacgcg gtgtgttcgg caacgtccgc accgcctttc atggcgaagc tgacgtttgc
     7321 ggcggcgagc gcgggcgcgt cgttgatgcc gtcgccgacc atcgccacgg ttttgccggc
     7381 ggctttgagt ttctgcactt cggcggcttt gtcgcgcgga ctcatattgc cgaaggcgtg
     7441 agcgataccc agttgttggg cgacgtattc gaccgtgcct tggttgtcgc cgctcatgat
     7501 gtaaacatcg atattgtgtt ttttcagacg gcctatggct tcggcggtat cggctttcaa
     7561 cgcgtcggca agtgcgaatg caccgatagg tttgttatcg actgagactg caacaatgct
     7621 tgcaatgtcc caaacgccgt ctgaaaactc gggcaaggtc agttcggcaa attcggcttt
     7681 gcccgctttc accaatcccg caccttccac ttcggcggca atgcctgcgc cgacaacggt
     7741 ttgcgcgttt tgtgcggcgg gaatctccaa accgcgcgct tgggtggcgg aaacgatggc
     7801 tcgggcaagc ggatgggcgg cgttttgttc gacggcagcg gcgatgcggt acaaatcgtc
     7861 ttcgtcaaag ccgctgtcgg gaacacaata aacggcggca acctgcggcc tgccttcggt
     7921 cagcgtgcca gttttgtcca acacaacggc atcgacgtgg gcggcttctt ccattgccgc
     7981 tgcgtcttta aaccaaatgc cgtgtttcac tgctttaccc atgccgacca taatcgcggc
     8041 gggtgtagcc agaccgagcg cacacgggca ggcaatcacc aaaacggcga cggcgtgcat
     8101 cagcgcaacc gtccaatcgc ccttaatcag ccaagtaaca ataaaagtca acaacgcgat
     8161 gcccacgacg gcaggcacga acaccgccgc cgccttgtcc gccacgcgcg caatcggtgc
     8221 tttactgcct tgcgcttcag agagtgcgtt catcatgtcg ccgagcaggg tttggctgcc
     8281 gagctgcgcg gcgcggtaca ccacgctgcc ttcggtcatc agcgcgcccg ccaacacttt
     8341 gccgcccgcc tttttctctt cgggattgga ttcgccggta agatggctct cgtccgccca
     8401 gccgctgccg ctttcgataa cgccgtcggc ggcaatgcgt tcgccgtggt tggcgcggat
     8461 aaggtcgccg atttgcactt ggtcgatggg aagctgtttc cattcgccat cgcgctgcac
     8521 gttgacttgg gtcggcgtga gtttgagcag caagcccaag ctgttcaggc tggatttttt
     8581 ggtacggtgt tccaaaaatt tgcccagcga cacaaaacca atcaccatca cgcccgcttc
     8641 aaaatacacg tgcgccattc cgtgtgccgc gtgcggactg aaaaacaaca tataaacgga
     8701 atacaggtaa atcgacaccg tgccgatagt aaccagcacg tccatattcg ccagcccgcc
     8761 tttaatgctc gcccacgcgc ttttgtaaaa cgggattgcc agccaaagtt gcacaatgct
     8821 tgccagcgca aactgccaca acggcggaat catccaatcg tgcctaccga tcatcatccc
     8881 cgccataccg acaaggaacg gaatattgat ggcggacagc agccacagcc gccagccgat
     8941 atggtgttct gcttcagctt gcggcaatgt atcttccgtt ttttccttcg cgccgtaacc
     9001 ggttttctca atgattttgg caatgtcggc tgcggaagtt ttgctgtcgt caaacgtaac
     9061 ctgcgcctcc tcgctggcga agttcacccc cgccgattcg acaaagtctt ttttgttcaa
     9121 cactttttca atgcgcgaag cgcaggcctg gcaggtcatg ccttcgattt ggaaacggat
     9181 tttttgttgc ataagcgtga ttcctgcttt gatattcaga cggcagttaa ggctgcggga
     9241 tgccgtctga gcgccggtat ggtcaaaatt tctcaaacgc gccgataatt cgagtagaat
     9301 ccgaaaactt gacacagcaa aggaaaaaca atgtcccaac accgcaaact gattattttg
     9361 ggttccggcc ccgccggata caccgccgcc gtctatgccg cgcgcgccaa tttaaacccc
     9421 gtcattatta caggtatcgc gcaaggcggg caactgatga ccaccaccga agtggacaac
     9481 tggcctgccg atgccgacgg cgtgcaaggg ccggaattga tggcgcggtt tctcgcccac
     9541 gccgaacgtt tcggaacgga aatcattttt gaccaaatca acgccgtcga tctgcaaaaa
     9601 cgcccgttta cactcaaagg cgatatgggc gagtacactt gcgatgccct gattgtcgca
     9661 acaggcgcgt ccgccaaata cctcggtttg ccgagtgagg aagcgtttgc agggaaaggc
     9721 gtttccgctt gtgccacctg cgacggtttc ttctacaaaa accaagatgt tgccgtagtt
     9781 ggcggcggca atacggcagt tgaagaggca ctctaccttg ccaatatcgc caaaaccgtt
     9841 accctgatcc accgccgcag cgagttccgt gccgaaaaaa tcatgatcga caaactgatg
     9901 aaacgcgtgg aagagggcaa aatcatcctc aagctggaaa gcaacctgca agaagtactg
     9961 ggcgacgatc ggggcgtaaa cggcgcatta ttaaaaaaca acgacggttc tgagcaacaa
    10021 attgccgtca gcggcatttt tatcgccatc gggcacaagc cgaataccga tattttcaaa
    10081 ggtcagttgg aaatggacga agccggttac ctgaaaacca aaggcggcac ggcggacaat
    10141 gtcggcgcaa ccaatatcga aggcgtatgg gcggcgggcg acgtaaaaga ccatacctac
    10201 cgtcaggcga ttaccagcgc ggcttccggc tgtcaggcgg ctttggacgc ggaacgctgg
    10261 ctgggcagcc aaaacatttg aatgccgtct gaaaccgaaa ttttcggcgg aatgcaaatt
    10321 tttcgcacca aatccttgat tccgtcggca aaccccaata taatgcctgc tctttgaacc
    10381 ttgccttctg ctttcgcatg gcagaaggct gttttaccat ccataaggag ataacatgcg
    10441 tcattacgag atcgtgttta tcgttcatcc tgatcaaagc gagcaagtgc ctgctatggt
    10501 tgaacgttac aaaaccatga ttgccgaagc aaacggtaag attcaccgtt tggaagattg
    10561 gggtcgccgc caactggctt acccgattaa caaaatccat aaagcccatt acgttttgat
    10621 gaacatcgaa accactcccg aagtggttga agagctggaa accgcattcc gcttcaatga
    10681 tgccgtattg cgccatctaa ccatcaaaac caaacacgcc gttactgaag catcccctat
    10741 gttgggtggt gaaaaggcta agaacctgtt gagcggtgcg tctgaagaag cggtcgccca
    10801 ataattggga ttcaataatc ttgtttcgct tgccgcgtta attgaaaagg ttttccctat
    10861 tcgatatacg cctgccggaa tccctgtttt agatattatt ttaaagcacg aatcgtggca
    10921 ggaggaaaac gggcagcaat gccttgtcca attggaaatt ccggcacgga ttttaggcag
    10981 gcaggcggaa gagtggcagt atcggcaagg tgtatatgtt cacgtcgaag gttttttagc
    11041 tcaaaaaagc agacgttccc ttatgccgat gctcaggata caaaatattc aagaatataa
    11101 aggttaaacg acaatggctc gtcaatcatt caaacgtaga aaattctgcc gtttcacggc
    11161 tgaaaaaatc caagaagtcg attacaaaca agttgatttg ctgaaagact ttatctctga
    11221 aaacggtaaa atcattcctg cacgcatcac aggaacgaag gcattctacc aacgccaatt
    11281 ggctgttgcc gtaaaacgcg cacgcttcct ggctctgctg ccttacaccg accaacacaa
    11341 ataattttgg agattgaatc atgcaaatta ttctgttaga aaaaatcggc ggtctgggca
    11401 acttgggcga tatcgtaacc gttaaaaacg gctacgcccg caactttcta attcccgcag
    11461 gtaaggcaaa acgtgcgacc gaagcgaata tgaaagagtt tgaagtacgc cgcgccgaat
    11521 tggaagccaa acaggctgaa attttggcag atgcccgagt ccgtcaggaa aaattggacg
    11581 gtcaaaccgt taccgttgct caaaaagccg gtgtggacgg tcgcctgttc ggttccgtta
    11641 ccaatgccga cattgctgct gcaatcgttg ctgccggcat cgaagccgtg aaagcaaatg
    11701 tacgtctgcc gaacggtcct ctgaaagccg ttggcgagta cgaagtggaa gtggctttgc
    11761 acactgatgc cgttgctaaa attaccgttg ccgtcgttgc cgcaaccgag taattgaaat
    11821 tatcagatgc cgtctgaaac cctgccgatt gtcggaatgt ggtttcagac ggtatttgtt
    11881 ttgttttccg tatattctgc aaaagtcggg atggttgttt ttatagtgga ttaaatttaa
    11941 accagtacag cgtacctggc ttcaatatac tcaataggat aggtctcttc ctaaccgaaa
    12001 aaataattgt cttttccctt gcaaataatt gaaatcaaca gattactaaa acacagccaa
    12061 aatattcaat tgacaaaaac ttttctgatt ttgagaaatc tgtgaaaatc aggggattat
    12121 ttattttgaa aatggtatcg ggcataaaat ttggctgtgt ttcaatatgg tgctgataac
    12181 atactttgtt tcaaaatgtt caataggata acttcctgat tgaccgaaaa ataatggcct
    12241 tttcccttgc aagcagttga aatcaacaga ttgtcaaaat acatcctaaa aatttaataa
    12301 cttatatata tgattgcaaa ggttgcggct tgtatgccgg tcattatggc ggaaataccg
    12361 tttgggaggc agccgcatcg cgtggaatcg gcacggcagg cgatgcgccg atgccttcgg
    12421 gcgtgaaggc ggggcaatcg gcagtttgga aaaaggcaat ggaatctgtg ggcagaagaa
    12481 gttaagggta aaagtcgggc gcacggtcag gttgtctgtc cttttgtcgg aacaggagac
    12541 tgggcagctt tgtcctgatt aattgcttgt gcaaacggta aaaaggtttt ttcgtgtgtt
    12601 ttaaagcagg ttgtacagca tttttccggc aatcaaaagc agcataatgc cgaaggattt
    12661 tttgagtttg gcagaagaaa gtttgtgggc ggttttgaca ccgagcgggg caaaggcaat
    12721 ggttgccgcg ctgaggacgg cgacggcggg caggtaaagg aagcccagtg acccttcggg
    12781 caatcctgca atattcaggc cgttgagcag atacgatatt gcgccggaga gtgcaatcgg
    12841 ccaggcaagg ccggatgatg tgccgatggc tttatgggcg gggaagccgc agtggattaa
    12901 gaaggggacg gaaagtgaac cgccgcctat gccgacccag ctcgacattg tgccgaacag
    12961 tgtggaaacc gcagtcagtc cgggcagctt gggcagcggg cgggatgccg tctgacgacc
    13021 ggtatgcagt gttttgaatg cgacggcggt taaaaacagg ataaagaaaa tttgaagccc
    13081 gaacgcgggg atatattttg cggagagtgc gcccgtgaat acgccgaata tcatacccgg
    13141 catcatcgca aatacggttt tccagtcgac cgcccgtttt ttgtgctgcc ccaacatact
    13201 ggaaaaggcg gtgaagacca tgacggcgaa ggatgtgccg acggcgaggt gttgcgcgta
    13261 aggatgttgt gccaaaccct gcaaatcaag cacccataaa acgacaggga caatcagcgt
    13321 gccgccgcct acgccgaaca gaccggcaat aaaacctgcc gcactgccta cggcaagcag
    13381 gattaagata atgtcccaat gccacattta atccgccttt ctgtattttt tccaaaatcg
    13441 gcgtgccgcc ataatgtagc cggacaggct gtatccgaga aagaacagga agaggacgag
    13501 cgacggttcc caagtgacca gaagcagcag taagacggca agcagcattc cgacaaaggg
    13561 gacttgtctg cggatgttga tttctttaaa actccaaaaa gggatttgga caatcatcga
    13621 caggccggca aacagtgtga tgcccaatgc ccaccagtgg acggcgggga atttttcgac
    13681 gctgtggttg acccaaatca gcccgacaat cagcgcggcg gcagtcggac tgggcacgcc
    13741 gataaaccag cgtttgtcca ccttgccgat gagtgtgttg aacagggcga ggcgcagggc
    13801 ggcgcaggcg cagtagatga aggcgacgga ataaccgatt ttgccgaact gccaaagctg
    13861 ccatttgtag gcaatcagag cgggagcaac gccgaagctg accatatcgg caaggctgtc
    13921 gagctgctcc ccgaacgcgc tttggctgtt ggtcagccgc gccacgcgcc cgtccatacc
    13981 gtccagcaac atagagatga ataccgcgat ggcggcggtt tcataacgtc cgtgcatgga
    14041 ttgggtgatt gcataaaatg cggaaaacag cgcggcgatg gtaaaggagt tgggcagcag
    14101 gtagatactg ttttgacgca gggagcgttg cggggtgttg gtcggggttt ccatagctga
    14161 agtgtctcac ggacgtggag aatcgatagg ggattataac ccaaaccatt atcgaacaat
    14221 acgcccttta ggatgaagcc ctcggaggcg gtttgttttt ctgatggcgg atgctttgcg
    14281 gggcggtttg actctgcctg ccaccgggtg tgttgtttcg gatttttgcc ggatggatat
    14341 tgttgccggg catttctggg cttgccggaa ggtatcgggg ggagggcatc ggaatatgcg
    14401 gcgcaccgtt caaaccgggg cggcgtttcc ctattttcag ggggttgcag gtttcaacaa
    14461 attgaaagat attttttgca tttccgaccg tatgctgatt ttgaagcggg ataaattata
    14521 ggacaaggtt tttcgcccgc cgcaaaggtt ggccgcaatg ccgtctgaaa acaacggctt
    14581 gccgctaagg cggcggtatg cggatttgcg ctgccgaaat cggtttgcca tacccggcgg
    14641 tgcaatctgc ggggagggat aagccgataa ggcgggactt atcctgtttt ttgtttataa
    14701 tgcaacagaa tttggacgaa ccgtcgggaa tttatcatga tgaaaaagat actggccgta
    14761 tcggcactat gcctgatgac tgcggcggca cgggctgccg atacttacgg ctatctcgcc
    14821 gtttggcaga atccgcagaa tgcggacgat gttttgcagg ttaaaaccac aaaagaagat
    14881 tcgacgaaaa gcgaagcgtt tgccgagttg gaagccttct gcaaaggtca ggacacgctt
    14941 gcgggcattg ccgaagacga gccgaccgga tgccggtcgg tcgtgtcgct gaacaatacc
    15001 tgtgtcgcgc tggcataccc gaaagccttg ggcgcgatgc gcgttgaaaa cgccgtcgtg
    15061 attacttctc cgcgttttac gagcgttcat caggtcgcac tcaaccagtg catcaaaaaa
    15121 tacggcgtac agggacaatg cggcttggaa acagtgtatt gcacatcttc ttcttattac
    15181 ggcggaactg tgcgctcttt gattcaaaat ctcaaataaa acggaaaatg ccgtctgaaa
    15241 gatgttcaga cggcatttct atatcgacgg tcaggattct ttcggatcgg gcagcaggct
    15301 gttcaacata atggcaagta cggcgcacaa gcccacgccg gcaaagctga agctgcccaa
    15361 tttgagcgtc atgccgccga tgcccgtggt cagtaccgag ctgacgatga ccaggttttt
    15421 cggcagcatc aaatcgactt tggcatcaat caacgttttc acgcccaaag aagcaatcgt
    15481 gccgaacagc agcagcataa tgccgcccat tactggcatc ggaatggaag ccaaaaacgc
    15541 attgaatttg ccgaaaaacg ccatgcagac ggcaaaaacc gccgcccaag tcatgatgac
    15601 ggggttgctg tttttggtaa tcatcaccgc acccgttact tcgccgtagg tcgtaaccgg
    15661 cgggccgccg atcagacccg caacgcatac gcccaaaccg tcgcctgcaa gggttttgtc
    15721 caagcccggg tctttcgtat agtctttccc cgtcacattg ccgattgcca tgatgccgcc
    15781 gatgtgttcg atggcggggg cgacggcaac gggcagcata aacagtgcag cctgccagtt
    15841 gacctgaggc gtttcaaaat ggggaacggc gaaccagggc gcgtgtgcaa tgcttgccgt
    15901 gtccaccagt cccatcagca gtgccaaaac ataacccgaa gcgacaccga tcaagatggg
    15961 aatcagcttc atcatcctgc tgccgaaaac cgatacgatg gcggtaacgg caaaggtaaa
    16021 gccggaaaga atcagcgaat cggtatagtc gatgacctgt ttgccgtccg cctgacccat
    16081 tgccatgctg cttgccgcca cggcaacaga cagaccgatg accatgatga cggggccgat
    16141 gacgaccgga ggcagcagtt tgtgtaccgc tgccagtccg cgccaacgga tcagcgcggc
    16201 aaacacaaaa tacataaagc cggcggtaaa cagtccgaac atggtggaag gcagccccca
    16261 ttcgccgacg gagtagataa tcggtgcgat aaaggcaaac gaagaaccaa gaaaaatcgg
    16321 cactttgcgt ttggttgtga tttggaacag cagcgttccc aagcctgcgc ccaaaagcgc
    16381 aagagccgga ttcagaccgg tcagcagggg aaccagcacc attgcgccga atgccacaaa
    16441 taaaatctgc gcgccggaaa cggcaagttt cagttggttc ataccatctt ctttcaatcg
    16501 gttcaaacgg cacggattat actggtttgc cgagggtctg cgtaaaaata catatggcgg
    16561 gcaacagata tgccgtctga aaggcaatgt ctgtctgttg caggatgaaa gcaggagaaa
    16621 tggagtataa tccgacactt tccatattca atccgcaagc cgtccggctt ccttatttca
    16681 gacggcattt tccggtaata cggcatgaca gaaaaatccc ataaaaaaac agcaaaaggc
    16741 agggcgggaa gcccgtcccc aacgtccgca cgcaacaaga aagccgacaa cggcgcacgg
    16801 ggcaataaag tttccgagcg gctcaaggcg gtcaaagagt tgcagaaaac cgaaaccaaa
    16861 aaggcgcgtc ccgaacatgt cgtcaacctt atcggcgacg cactgtggct gatgggtttg
    16921 gcggcaaccc tgtatttggc gatttccctg atcagtttcg atatgggcga tccgtcttgg
    16981 tcgcacagtt cgccggttgt ggaagatgtc gccaattggg gcggactgtt cggcgcgtat
    17041 gttgccgatg tcggctatta tcttttcggc tggtcgttct ggtggtggat agcggctgcc
    17101 tgcgtcatgc tgtataaaaa ttttcgtctg cacgcaaaac agacggaaaa cgaggcatac
    17161 aaccacaaaa tcgctgccgc cgcgctgttt gtcctgacgg tcttcagccc cgtcttggag
    17221 tattttgtgc tgggcggaaa atatgccgac tccctgcctg tcggagcagg cggtatggtc
    17281 ggcatacgcg tcggcgcagt gtttgcgtgg ctgctgggga aatcgggcag cctgctgatt
    17341 atcttggttg ttctgctgtt gtcgttgtcc ctgctggtgc agatttcatg gctggaattt
    17401 ttgaacggtg cgggcagggc ggttcaaaac cgcctgagtg ccttatccgg caaggtcatg
    17461 gctttaggaa aacgccggcc gaatagcaaa acagacggtg tcgataccca aaatacacgg
    17521 cgcatggtaa aagaagccaa gaatattacg gccaaacccg ttgccttgcc cgaaggcagc
    17581 agcagcaacc gcaaatccgt cgcggtttcc gtcgcgccgt cgcccaaaat tcaggtttct
    17641 ctgtttgaag atgacgaacc tcggcaggcg ggcgaatacc acaagcctac attgaaccta
    17701 ttgcggattc ctgacagcga acccgtcagc atcaatcccg ccgaattgga gcgcactgcc
    17761 gaactgatcg aatccaaact ggcagaattc ggcatcggcg tacaagtcgt atccgccaca
    17821 tccggtcccg tcatcacgcg ctacgaaatc gaacccgcgc aaggtgttaa aggcagccaa
    17881 attgttgcct tgtcgaaaga tttggcacgc tctatgtcgc tgcaatccgt gcgtatcgtc
    17941 gaaaccatcg caggtaaaaa cacgatgggc atcgagttgc ccaacgacaa acgccaagac
    18001 gtgatgttga gcgaaatctt gtcctcgccc gtgtttgccg aagccaaatc caagctgacc
    18061 gtcgcgctgg gcaaagacat tgccggtacc cccgttgtcg gcgacttggc gaaaatgccg
    18121 caccttttgg tcgccggtat gactggttcg ggcaagtccg tcggcgtgaa cggcatgatt
    18181 atgtctatgc ttttcaaagc tacgcccgac gaagtccgct ttatcatgat agacccgaaa
    18241 atgcttgagt tgagcattta cgacggcatt ccgcacctgc tctgtcccgt cgtgaccgat
    18301 atgcgcgaag cggggcaggc gttgaactgg tgcgtcgccg aaatggaaaa acgctaccgc
    18361 ctgctttccc acgccggtgt acgcaatctg gaaggcttca accaaaaagt cgaagccgca
    18421 aaagcggcag gcaagccgct gctcaatccg ttcagtctga accccgacag ccccgagcct
    18481 ttggaaaaac tgccgatgat tgtggtcgtt atcgacgaac ttgccgacct gatgatgacc
    18541 gaacgcaaag ccgtcgagca gcaaatcgcc cgtctcgccc aaaaagcgcg cgccgccggt
    18601 atccatatga ttgtcgccac ccaacgtccc agtgtcgatg tcgttactgg cctgattaaa
    18661 gccaacatcc cgacgcgtat ggcgtttacc gtgcaaagca aaatcgacag ccgtaccatc
    18721 ctcgaccaaa tgggcgcgga cgaactgctc aaatatggcg attcgctgtt cctccagccc
    18781 ggcagtgccg aaccgacccg cctgcaaggc gcgtttgttt cagacgacga agtacatcaa
    18841 gtcgtcaact acgtcaaatc gcaagcccca gccgactata ttgaaggtct gctcagcggc
    18901 gaggccgcgc tggaaactgc caatatcgtt aatccgaatg cagacagcga cgaattgttc
    18961 gatcaggcag tcgcctatgt tttggaaagc aaaaaaacct ccatttcgtc tttgcagcgg
    19021 cagttgcgta tcggctataa ccgcgcggca aacctgatgg aggcactgga aaatgcgggt
    19081 gtcgtttctc ccaccgacct caacggcagc cgtaaaattt tggcgcacaa ggaccatttg
    19141 tagctcgtat tgcaaaatgc cgtctgaacg gcggaattgg cgtttcagac ggcatattat
    19201 gtttcaggcg gaacattgtg atatacttgc cagctaaaat ctcccctttg cggcaatgcg
    19261 gttcaaatat ccgtaccgtt gcgctgtttg cttccccatg cagggaagaa agtttaccat
    19321 tttatcaaca caacaaattt aagggcttat gatgagcgta actgttgaaa ctttagaaaa
    19381 tctggaacgc aaagtagtgt tgtccctgcc ttggtccgaa atcaacgcag aaaccgataa
    19441 aaaactgaaa caaacccagc gccgtgcaaa aatcgacggt ttccgcccgg gtaaagcacc
    19501 tttaaaaatg attgcccaaa tgtacggtgc gagcgcgcaa aacgacgtga tcaacgagct
    19561 ggtgcaacgc cgcttccacg atgttgccgt tgcccaagag ttgaaagtgg caggcttccc
    19621 ccgttttgaa ggcgttgaag aacaagacga taaagaatct ttcaaagttg ccgccatttt
    19681 tgaagtgttc cccgaagtcg ttatcggcga tttgtctgca caagaagttg aaaaagtaac
    19741 cgcttccgtc ggtgatgccg aagtggacca aaccgtagaa atcctgcgca aacaacgcac
    19801 ccgcttcaac catgtcgaac gcgaagcccg aaacagcgac cgcgtcatca ttgactttga
    19861 aggcaaaatc gacggcgaac cttttgcagg cggcgcatcc aaaaactacg ccttcgtatt
    19921 gggcgcaggt caaatgctgc ctgaatttga agccggcgta gtcggcatga aggctggcga
    19981 aagtaaagac gttaccgtca acttccctga agaataccac ggcaaagacg ttgccggtaa
    20041 aactgccgtg ttcaccatta cgctgaacaa cgtttccgaa gcgactctgc ctgaagtcga
    20101 tgcagatttt gcaaaagcct tgggtattgc ggatggcgac gttgccaaaa tgcgcgaaga
    20161 agtgcagaaa aacgtaagcc gcgaagtgga acgccgcgta aacgaacaaa ccaaagaatc
    20221 cgtaatgaac gcgctgctca aagccgtaga gctgaaagca cctgttgctt tggtcaatga
    20281 agaagccgca cgcttggcaa acgaaatgaa acaaaatttt gttaaccaag gtatggctga
    20341 tgctgccaac ttggatctgc ctttggatat attcaaagaa caagccgaac gccgcgtatc
    20401 tttgggtctg attttggcca aactggttga tgaaaacaaa ctggaaccga ctgaggagca
    20461 agtcaaagcc gttgtcgcca acttcgcaga aagctacgaa gatcctcaag aagtaatcga
    20521 ctggtactac gcagagcctt cccgcctgca agccccgact tctttggcga tagaaagcaa
    20581 cgtcgttgat ttcgttttgg gtaaagccaa agtaaacgaa aaagctttgt cttttgacga
    20641 agtgatgggc gcgcaagcct gattatcctg aaaatgccgt cttagggcag tttttgaaag
    20701 caccgaagcc gcttttaaag cccgctttgg tgcttttaca tcatgaaagg agacgaaatg
    20761 tcttttgata actatcttgt ccctaccgtt atcgagcaga gcggtcgcgg tgagcgtgca
    20821 ttcgatatct attctcggct tttgaaagag cgcatcgtat tcttggtcgg cccggtaacc
    20881 gacgagtccg ccaatctggt ggttgcccaa ctgttgtttt tggaaagtga gaatccggat
    20941 aaggatattt tcttctatat taactcgcca ggcggttcgg taacggccgg tatgtcgatt
    21001 tacgacacca tgaattttat caagcccgat gtatcgactt tgtgcttggg gcaggcagca
    21061 agtatgggcg cgttcttatt gtcggcaggc gaaaaaggca aacgtttcgc cctgcccaac
    21121 agccggatta tgattcacca gcctttaatc agcggcggct tgggcggtca ggcatccgat
    21181 attgaaattc acgcacgcga actgttgaaa atcaaagaaa aactcaaccg cctgatggcg
    21241 aaacattgcg gccgcgattt ggcagatttg gagcgcgaca ccgaccgtga taatttcatg
    21301 tccgccgaag aggctaaaga atatggtctg attgatcaaa tcttggagaa ccgtgcttct
    21361 ttacagtttt aataagaaat ctgagagaaa caaatgtcgt ctgaaaacag ttcaggacgg
    21421 catttgtctt atagggactg tatagggatg attttgttct ggttgcgttt ggagccgttt
    21481 tcaccatagt tggttttgac atattcctct acaatagcta ggaattcagt tgcaatatta
    21541 tcacctttca gtgttatttt gcgctccccg tccacataaa ccggcgcgac gggtgtttct
    21601 cccgtaccgg gaaggctgat gccgatgtcg gccaatttgc tttctccggg cccattgaca
    21661 acgcagccca ttacggcaac gttcagggat tcaaccccag gataaagggt acgccatata
    21721 gacatttttt ggcgcaggta attttgaaca tcttgtgcca gctcttgaaa tacggtactg
    21781 gtggtacgcc cgcagccggg gcaggcggta accatcggcg taaacgaacg caatcccata
    21841 gtttgtaaaa tctcttgccc gacgacgacc tcctgagtac gcgggctgcc aggttccgga
    21901 gtcagtgaaa tgcggatggt gtcgccgatt ccttcttgaa gcaagacgga taatgccgcc
    21961 gttgatgcga caatgccttt gctgcccata ccggcttcgg tcaaacccaa atgcagcgga
    22021 taggcgcaac ggctgcccag ttcgcggtaa acctgaatca aatcctgaac cgcgctgact
    22081 ttgcacgaca ggatgatttt gtcttcgggc agtcccaata gaacggcttt ttcggcagat
    22141 tccaaagcgg agacaatcag tgcttccttc gtcacttctt cgggcggttt cggcgcggaa
    22201 gaagcgaggt tggcatccat catacgtttg gcgaggcttt gatccaaaga accccagttt
    22261 acgccgatgc ggacggcttt atcgttttca gcagcagtcc gaatcataaa ggcaaatttt
    22321 tcatcgcctt ttacgccttt gccgacattg ccgggattga tgcggtattt ggacaatgct
    22381 ttgccgcatt cgggaaattc cgccaacagg cgttcgccgt tgaagtggaa atcgccgata
    22441 agtggtgtgg catagcccat atcgtccaag cggcggcgga tttcggcaac tttggacgcg
    22501 gcttcggggc tgttgacggt aatacgcacc atttcggatc cggcatcgct caattcctta
    22561 atctgcaatg cggtggcttt tgcatcggca gtgtcggtgt tggtcataga ttggataacg
    22621 acgggtgctt ctgaaccgac ggtaatatga tcgatgcgga cttgatgcgt cttgcggcgt
    22681 tggagtgtgt tcatatggtt tgaatctgtt tattgaccga tgaggacggt ttgcaattct
    22741 tccgagtagg ggaaattcgc ctgcaattgt gcttcatatt cgtatgccgc ctgtgcgttg
    22801 ccgagggctt tggcaatttt ccagcctagc agcaaatcat cggcctgaag gacttctacc
    22861 ctgctttggt attttttaaa gtagtaatcg gcatcgccca actgcccggc cagcattttg
    22921 gtgcgcgcca gttctttaaa tgcgggtggg aactgcggct gggcggcgag ggaacgtttc
    22981 aaataggctt ccgccaatcc gaattgcccc tgttttgcgc tgcatatgcc tttattcagg
    23041 ttggcaatat aaggggtcgg gtaggtgggg tcggccaggg ctttgtcgaa atatgccata
    23101 gattcggcag ggcggttgag cctgccgcac aggaaccagc cgtagttgtt gttgatttcg
    23161 gcactgtcgg gtttgatgga gagggcttgc cggaaacttt cctgcgcctt gtcgttaact
    23221 ttcaggtatt gatagatttc ggcacggacc agccaggcaa gctcgttttt agggtccgat
    23281 ttcagggcgt cttcaatact tgccgtcgcc tgacggtagt cctgaccgcg catatattcc
    23341 attgccaact gggttttgat attggaaacc tgattggctt tttctgcccg cgaggggcgg
    23401 taggaagtgc tgcacgcgcc caaggcaagg acaagtaata aagagattcg tttggatggc
    23461 ttaaaaggca taattacccc tgttgtccga ttaaaatctg ctgccatttt tgttggcggc
    23521 gcgttttatc ctgaacctgc cccgccaact gtccgcaggc ggcatcgatg tcgtcgccgc
    23581 gcgtttttcg tacggtaacg acaaatcctg cctgctgcaa aatatcgcgg aacacacgga
    23641 tgttctcatt gctggagcgt tcgtatccag agtttggaaa gggattgaac ggaatcagat
    23701 tgaacttgca gggaacatct ttgaccagtt cgatcagttc gcgcgcatgt tgcgccttat
    23761 cgtttattcc gtccaacatg acgtattcga aagtgatgaa atccctgggt gctttgacta
    23821 gatagcgttg gcatgcagcc atcaattctt tcaagggata ttttttgttc aacggtacga
    23881 tttggttgcg gacttcgtca ttggaagcgt ggagggaaac cgccaaagcc accggcatga
    23941 catcgcgcaa cctgtccatt tggggaacca tacccgaagt ggaaacggtt acgcggcggc
    24001 ggctcaaacc gtagccgtgg tcgtccagca tgatgcttaa ggcggtaacg acattgtcga
    24061 agttcgccat cggctcgccc atacccatca tcactacatt ggaaattacg cgttcgtttt
    24121 tcggtgtaac acccatcgct ttgtttgccc accacaattg cccgatgatt tcggcagcag
    24181 tcaaattgcg gttgaagccc tgccggccgg tcgaacaaaa tgtacattcc aaagcgcagc
    24241 cgacttgtga ggaaatgcag agcgtgccgc gatccgattc ggggatgaag acggtttcca
    24301 ccccgttgcc cgtaccgaca tccaaaagcc attttcgagt gccgtctgaa gatttttgag
    24361 acatcatcag cttgggaatt tcgatgcctg cctgttcgtt cagtttatgg cgcaacgatt
    24421 ttgccaaatc ggtcatttcg ttaaaatttt gcgcgccgga ttggtgcatc caacgcataa
    24481 cctgtttggc acggaagggt ttttcgccca tatcggcaaa atgtcgggtc agcccttgaa
    24541 ggtcgtagtt gagcagattg gttttcatgt gtttgtgttt ttcttaaatt aaggcttcaa
    24601 ataaaaaatt gcagggcagg caagtttgat ttgaagcctt ttgcgacaga gttttcagac
    24661 ggcatggata tatttatgcc gtctgaagag gagttgtgcg gtgtattgta tcaacggggg
    24721 cagatttcgg tttggctgaa aaagtaggca atttccaaag cggcattttc cacgctgtcg
    24781 gaaccgtgta cggcattaat gctgaccgaa gtggcaaagt ccgcgcgtat cgtgccttcg
    24841 gcggcttcgg aaggattagt tgcacccatc agttcgcggt ttttcaggac ggcgttttca
    24901 ccctctaata cctgaatcat aaccggaccg ccggtcataa attcaaccaa tccggcgtag
    24961 aaggggcggt ctttatgaac cgcataaaat tcttgcgcct ctttgagagt aagctgcttc
    25021 attttggcgg caacgatttt cagaccgttc tcctcaaagc ggctgtatat tttgccgata
    25081 acatttttgc cgacggcatc gggtttgatg atggagatgg tacgttcaat cgccatgcta
    25141 tatccttatt tttacttaag aagaatcaaa tcggtattct atcaaaaaaa taacttatcc
    25201 gcctgaagcc ttgggagata gggtcaggtg cggctgttcc aaataaactt cggactgcat
    25261 ttcgaccatc cggctggcgg tgcgggtaaa ttcttcggca aaatcgcctt ccgtatagat
    25321 atggttgaca tctaccgcgc cggtggcaca cagtttgacc cggaaatcgt agagtacgtc
    25381 aatcagccaa gtcagccgcc gcgcctccgc cttttcttgc ggtgagagtt gttccaaacc
    25441 tgaaataaaa accatttcat aatgttcggc caaatacaga tagtcggact gtgagcgggg
    25501 gccgaagcac agtgcgcgga aatcaaacca tatggcacgg ccggactcgg ctttgtgggg
    25561 aatctcccga ccatggatgg tgctgatgcc ggggttcaaa tcggtaatgc ctgtcatttc
    25621 tttgaacagt tttgccagtt ttgcctcatt ttcttcattg gcaggcgtaa agaaaatctc
    25681 ggcggggcgg agggtacgca gtcggtagtc ttcaccgccg tcaacgttta agacggtcag
    25741 gctggactcg atgagcgcga ttgtgggaag aaaactgctc cggttttgac cttgcgggta
    25801 gagttcggaa ggcgcgtagt ttgaagtcgc caccaaaaca acgccctcgt taagcaggtt
    25861 ttccagcaga cggcctaaaa tcattgcatc cgcaatatcg ctgacatgaa attcgtcaaa
    25921 acataatacg cgggtttctt tggcaatctc ggcggcaacg gatttcaacg ggttgctttc
    25981 gcttttcagg gttttcagcc tctggtggat ttctgccata aaggcatgaa agtggacgcg
    26041 gcgtttgcgg cggtaaggga ggcagccgaa aaaagcgtcc atcagaaagc ttttgccgcg
    26101 tccgaccccg ccatagaaat aaagcccttt ggggacttgc ggggaacgca aactcctgcc
    26161 taaaaaacgg tttcttttgc gtttgaacat catcaattcg gtccaaagcc gatcgaggtg
    26221 ttcgatggcg gctgcctgcg cgtcgtcgcg gatgaagttg ggcagttgtg aggcagcctg
    26281 ataccaggtc agcgggctgt ggttttcaaa cggcggggct ttaaaaagtt ggtctctatt
    26341 actcatattt aaccttgtta tacttcttgc ggcggagaaa gaaaatgctt accgtcggta
    26401 ttataaatga tttccttcaa acttggcatt ttccgtggaa aagaataatg acgcgcacaa
    26461 gggggtataa gcaaaggcgg cgtttcaata tggtgcagat atgagctcaa atgcctttta
    26521 tagt
//
LOCUS       DAWKST010000006        26491 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.006, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000006 DAWKST010000000
VERSION     DAWKST010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26491
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.006"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(226..549)
                     /locus_tag="WJL87_000159"
     CDS             complement(226..549)
                     /locus_tag="WJL87_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ7798653.1"
                     /translation="MDIVQLGSAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(629..1285)
                     /locus_tag="WJL87_000160"
     CDS             complement(629..1285)
                     /locus_tag="WJL87_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ7798654.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMVVIVGRKPVQRALLITRKGDVFEEKVLFDTLVAHLDDKDADPFGGFQF
                     "
     gene            complement(1387..1890)
                     /locus_tag="WJL87_000161"
     CDS             complement(1387..1890)
                     /locus_tag="WJL87_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ7798655.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVDA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGELAKILEKRF
                     EASRQAV"
     gene            2074..2847
                     /locus_tag="WJL87_000162"
     CDS             2074..2847
                     /locus_tag="WJL87_000162"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ7798656.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2854..3204
                     /gene="secG"
                     /locus_tag="WJL87_000163"
     CDS             2854..3204
                     /gene="secG"
                     /locus_tag="WJL87_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ7798657.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKYGLDFSNVQQTQQAPKPI
                     SNTEPSAPVPQQQK"
     gene            3218..3303
                     /locus_tag="WJL87_000164"
     tRNA            3218..3303
                     /locus_tag="WJL87_000164"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3252..3254,aa:Leu,seq:gag)
     gene            4104..4472
                     /locus_tag="WJL87_000165"
     CDS             4104..4472
                     /locus_tag="WJL87_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ7798658.1"
                     /translation="MRIFKNQWIVKFAKKHKISDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
     gene            4469..4780
                     /locus_tag="WJL87_000166"
     CDS             4469..4780
                     /locus_tag="WJL87_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ7798659.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(5550..6104)
                     /locus_tag="WJL87_000167"
     CDS             complement(5550..6104)
                     /locus_tag="WJL87_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798660.1"
                     /translation="MIIGGIGGARTQTGLRFEERTDLRTLFEEIPGYDLRKTYDNAGY
                     EVWFNGELKAYCFKKYEFYRFLKRPGYNINWKDHLSKRLLPDNGLFIIIRDTLFIIEI
                     KFQQTPGSVDEKLQTCDFKRKQYTKLVHSLGWRVEYVYVLNDWFTKPEYKDVLDYIIS
                     VNCHYQFNTIPLRWFGLPDGETNE"
     gene            complement(6101..6910)
                     /locus_tag="WJL87_000168"
     CDS             complement(6101..6910)
                     /locus_tag="WJL87_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ7798661.1"
                     /translation="MIDFDKPAEEDAIYQSRLKKIVSDDLVLYNENSLNVMRKILEKY
                     PNGCFDMIFSDPPYFLSNDGFSCQNGQMVSVNKGNWDKSKGMAADLEFYEEWLRLCYA
                     LLKPNGTIWVCGTFHNIYLIGYLMQTIGYHILNNITWEKPNPPPNLSCRFFTHSTETI
                     LWAKKNKKAKHTFHYEMMKAQNDGKQMKCVWTFPPPNKAEKTFGKHPTQKPLPLLERC
                     ILSASNIGDLIFDPFMGSGTTGVAALKHGRKFCGCELEEDFFELAKKRLEK"
     gene            complement(6903..7715)
                     /locus_tag="WJL87_000169"
     CDS             complement(6903..7715)
                     /locus_tag="WJL87_000169"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HEZ7798662.1"
                     /translation="MASKPFLKWAGGKHKLVPFIEHNLPTPTRKRLIEPFCGSAALSL
                     ALDFEHYLLNDINADLIGLFRILKEEKSGFIDYARSFFISENNSDSRFYELREQFNFS
                     KNLHERSALFIYLNRHAFNGLCRYNSKGVFNVPFGRYKSPYFPQQEMEGFIQKSDRVE
                     LMCGDFQMILSQTDNTDTVYCDPPYAPLSPTASFTTYAKESFNLDNQIRLAQSAQQIA
                     PDSQGVLISNHDTEFTRDIYSFAILKTIEVQRNIAAKGSSRKKVGELLAIYD"
     gene            complement(7953..10583)
                     /locus_tag="WJL87_000170"
     CDS             complement(7953..10583)
                     /locus_tag="WJL87_000170"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ7798663.1"
                     /translation="MQEHYQPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     AAAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYNLPKKQVIAVGDNAFDANRWQEWYADKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     QCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETACPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVDPAAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPISAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGEAVKAFAGSQDGLSKELKDLRHKLHATTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGGEQGRAVAQEVLETAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            10850..11365
                     /locus_tag="WJL87_000171"
     CDS             10850..11365
                     /locus_tag="WJL87_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ7798664.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNNRGTFPTYDELDLMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEGITTCQYKIIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            11372..11815
                     /locus_tag="WJL87_000172"
     CDS             11372..11815
                     /locus_tag="WJL87_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ7798665.1"
                     /translation="MPFRLFLGILCGLSMVAPVYAQGQPDAVNAYIQKKKVIVNTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            11984..14041
                     /gene="ppk1"
                     /locus_tag="WJL87_000173"
     CDS             11984..14041
                     /gene="ppk1"
                     /locus_tag="WJL87_000173"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ7798666.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGTQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPIAAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            14066..14335
                     /locus_tag="WJL87_000174"
     CDS             14066..14335
                     /locus_tag="WJL87_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798667.1"
                     /translation="MPSETFPFQTAWFYSNLNRADWNQKHTFAIPAQALPLYAPNPNP
                     PHSRRHCLSDSRTKPRIRLHGSGHISNTGIVLRKTVKSASASQYI"
     gene            complement(14304..15407)
                     /locus_tag="WJL87_000175"
     CDS             complement(14304..15407)
                     /locus_tag="WJL87_000175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ7798668.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGADDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQSGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(15487..17043)
                     /gene="dnaA"
                     /locus_tag="WJL87_000176"
     CDS             complement(15487..17043)
                     /gene="dnaA"
                     /locus_tag="WJL87_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ7798669.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPHEPRHTAEPASRPESEV
                     LAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            17336..17470
                     /gene="rpmH"
                     /locus_tag="WJL87_000177"
     CDS             17336..17470
                     /gene="rpmH"
                     /locus_tag="WJL87_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ7798670.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            17473..17838
                     /locus_tag="WJL87_000178"
     CDS             17473..17838
                     /locus_tag="WJL87_000178"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ7798671.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVHRKFDRSTAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            17903..18124
                     /gene="yidD"
                     /locus_tag="WJL87_000179"
     CDS             17903..18124
                     /gene="yidD"
                     /locus_tag="WJL87_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ7798672.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPLGGHGHDPVP"
     gene            18296..19933
                     /gene="yidC"
                     /locus_tag="WJL87_000180"
     CDS             18296..19933
                     /gene="yidC"
                     /locus_tag="WJL87_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ7798673.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGRQSVCAAGECNIDI
                     KRRNDKLYSTSVSVPLAAIQNGAKAEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIIVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(20014..20724)
                     /locus_tag="WJL87_000181"
     CDS             complement(20014..20724)
                     /locus_tag="WJL87_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEZ7798674.1"
                     /translation="MSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAEKTARA
                     HLKHLGVTTPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGAN
                     LVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQAFRALEQR
                     SRQCGETQIFIETPYRNDALLADAVENLHPETRLCTATDLTLPTQEIISQTIVQWRKR
                     KEMPNLKKRPTIFVMYAG"
     gene            complement(20786..21376)
                     /gene="maf"
                     /locus_tag="WJL87_000182"
     CDS             complement(20786..21376)
                     /gene="maf"
                     /locus_tag="WJL87_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ7798675.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLTGRFPEALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAIVLLNTVTGRMRRHIDKTVVVMRQLDELHILRYLEREPDAVYCSCALKSEDL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            21524..22027
                     /locus_tag="WJL87_000183"
     CDS             21524..22027
                     /locus_tag="WJL87_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ7798676.1"
                     /translation="MSDPNLIDLEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSN"
     gene            22061..22240
                     /gene="rpmF"
                     /locus_tag="WJL87_000184"
     CDS             22061..22240
                     /gene="rpmF"
                     /locus_tag="WJL87_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ7798677.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            22415..22942
                     /locus_tag="WJL87_000185"
     CDS             22415..22942
                     /locus_tag="WJL87_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ7798678.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            23030..24085
                     /gene="plsX"
                     /locus_tag="WJL87_000186"
     CDS             23030..24085
                     /gene="plsX"
                     /locus_tag="WJL87_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ7798679.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDESPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENADGL"
     gene            24221..24472
                     /locus_tag="WJL87_000187"
     CDS             24221..24472
                     /locus_tag="WJL87_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ7798680.1"
                     /translation="MKTLEKRMKALDKQMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            24499..24915
                     /locus_tag="WJL87_000188"
     CDS             24499..24915
                     /locus_tag="WJL87_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798681.1"
                     /translation="MKTSTVVFGGFFMADNGERIQIPVLENPDIREINHFFSVSNFEK
                     KTGVLVFRIIPEPEFGNTELTVYFEKGYYLPMIQTILEGGDIEVKNLKTENYSGNTME
                     IWGDSYPIEHISKNISTIQNIISEFMIQKQTPAPII"
     gene            24935..25138
                     /locus_tag="WJL87_000189"
     CDS             24935..25138
                     /locus_tag="WJL87_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798682.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPALPVHGNLHLKTICIPNHTPI
                     PVSVICTLPVLGNHRFFAYNSRI"
     gene            25165..26127
                     /locus_tag="WJL87_000190"
     CDS             25165..26127
                     /locus_tag="WJL87_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ7798683.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSAADKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            26174..>26491
                     /locus_tag="WJL87_000191"
     CDS             26174..>26491
                     /locus_tag="WJL87_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEZ7798684.1"
                     /translation="MKRQAFFKPIACAAFLSAVSLRLPVLGACYAILSLYAFALYSID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TLIL"
BASE COUNT         6716 a         7541 c         6363 g         5871 t
ORIGIN      
        1 gcctgcacat tgaaaatgcc gcccaagcaa accttcagtt tgccagcctc gtcctagccc
       61 tctcccacgg ggagagggga ttggggtgca ggctgccttt aaggttcagg caaattttaa
      121 cttcgttgat accgcgcttt agcttcgcag aagctgcact ttcagacgac cttttggaac
      181 accacaggta cacgcattta aggaatgccg tctgaaatgc ctgcctcaat aacgcatcat
      241 gttgccgtca atctcggccg cccatgcatc gatgccgccc tgaaggttgt acaggttttc
      301 aaaacccgcg tccgccaaat acatcgccgt atgcaggctg cggataccgt ggtggcaata
      361 caccacaagc ggcacatcat ccggcagctc gttctgccgc agcggaatca gattcatcgg
      421 gatatgcagc gcatttggca gcgaacaaac cgccgtttct tcatccgtac gcacgtccaa
      481 caaacaaaac atccgccctt cgtccatcca cgccttcaat tccgccgacc caagttgcac
      541 aatatccatc gcacccccca aaaaaaaacc aagcaaaatg ccgtctgaag ccccaaaccc
      601 gctttcagac ggcatcgcaa tccgaacatc aaaactgaaa accgccaaac ggatcggcat
      661 ccttatcatc caaatgcgcc accaaggtat cgaacagcac cttctcttca aacacatcgc
      721 ccttgcgcgt aatcaaaagc gcgcgctgaa caggcttgcg gcctacgata accaccatgc
      781 gtccgccatc tttcaactgt tctttcaaca cttcaggcac aaggtttacc gcaccgccga
      841 cataaaccgc atcaaacggc gcacctgcgg aaagttcggt caacccgttg ttttgcacat
      901 aatcgatatt gtccaaaccc aagccgtcca acaccgcttt ggcgcggttt tgctgttcgg
      961 catcgatgtc gtccgacacc acacgaccag ccaattttgc caacagcgcg gtcgcatagc
     1021 ccgaacccgt gccgatttcc aaaaccgtat cgtttttcgt cagcttcaag ccctgcgcca
     1081 gccgcgccac gactttcggc tcgagcatct tatgaccgtt ggcaagcggc agcgccatat
     1141 ccgcatacgc caaaccctgc aagtcctcat cgacaaaaag ctcgcgcgga atctccgcca
     1201 aagcgtccaa aacgtcaaaa tccaatacat cccaaggacg gatttgctgt tcgaccatat
     1261 tgaaccgcgc tttttcaaaa tccatttagt gctccgtcaa ataattgttc caacaatagc
     1321 aggcattata aaaccacact cccgccgcgc caacttcggc acgcgccgcc cgcccctttc
     1381 cctcactcaa accgcctgac gcgaagcctc aaaccgcttc tccaaaatct tcgccagctc
     1441 gcccaaatac aaagcatctg tctcatcaaa ctgcgccaaa tgttcgctgt ccgcgtccaa
     1501 cacgccgata cagcggccgt ctgaaaacag cggcacgaca atctccgaac gcgacaaaga
     1561 cgaacaggca atatggtcgg gatgcgcgtc cacatccccg acgaccaccg tcccgccctt
     1621 cgcccaagcc tgaccgcaca ccccgcgacc gaacggaatc cgcgtacacg ccaaaggccc
     1681 ctgaaacggt gccaaaacca attcgtccga acgcgtatcg accaaataaa aacccaccca
     1741 aaaccaaccg aacgcctcct tcaaaaccgc cgccgtgttc gccaaattcg ccacccaatc
     1801 cgtctcgtcc gccaccacag actcaatctg cggcaacacc tcccgataaa gcgcggcctt
     1861 gtccgaagcc gaaaaatgaa gcgcgtgcat cacatctcct atagttgcat acatatcagg
     1921 cggccattat aaaacaacct gcccgaaaca acattccaaa ccgcccccgc agccgcccgc
     1981 cccaagttgc gaacccgccg catatccact aaacttcaca ttgcaacgcg ccacacgcgg
     2041 cagacagaaa aaacacgaca cggagcaaaa aagatgtatc accaaatcgg aatgtgggat
     2101 caaaaatggg tcatcggcaa ctggaaaatg aacggccggc tccagaacaa caattcgctc
     2161 atgcaccgct tccgcatcct ccccaccgcc gaacgcgtcc tcatcggact cgccgccccg
     2221 accgtttacc tgctgcaact gcacaacgcc atgcaaatcg tcttaaacaa ccgcatcctc
     2281 acctgcgccc aagacgtgag ccgcttcccc gacaacggcg cgtacaccgg cgaagtgtcc
     2341 gccgaaatgc tcgccgacac cggcacagac atcgtcctca tcggacactc cgaacgcagc
     2401 ctttatttcg gcgaaaaaaa cgaaatccaa cgccgcaaaa tggaaaacgt cctcaacgtc
     2461 ggactcatcc cgttattgtg cgtcggcgaa agcctcgaag agcgcgaagc cggcaaagaa
     2521 cacgaagtca tcgcccatca gctttccatc ctgcaagggc tggataccaa aaacatcgcc
     2581 gtcgcctacg aacccgtctg ggcgatcggc accggcaaag tcgccaccgt cgaacagatt
     2641 gccgatatgc acgcattcat ctacaaagaa atcttgtctt tgtgcggaag cgatgttaaa
     2701 atccgcgtcc tttacggcgg aagtgtgaaa gcggacaacg cggccgacat cttcgcagta
     2761 ccttatgtgg acggcgcact cgtcggcggc gcgtcattgt cgtacgactc ctttaccgcc
     2821 atcatcagtg ccgcacaaaa tgcgtagaaa aatatggaag ccttcaaaac cctaatttgg
     2881 attgttaata taatttccgc tttggccgtc atcgtgttag tattgctcca acacggcaaa
     2941 ggcgcggatg ccggcgcgac cttcggatcg ggaagcggca gcgcgcaagg cgtattcggc
     3001 tctgccggca acgctaactt cctcagccgt tcgaccgccg ttgcagcaac atttttcttt
     3061 gcaacctgca tggctatggt gtatattcac acccacacga caaaatacgg tttggacttc
     3121 agcaacgtac aacaaactca gcaagcaccc aaacccataa gcaataccga accttctgcc
     3181 cctgttcctc agcagcagaa ataacagttt ttcaaatgcc gacatggtga aattggtaga
     3241 cacgctatct tgagggggta gtggccgtag gctgtgcgag ttcaaatctc gctgtcggca
     3301 ccaacacaca aaacgcttag aaattctaag cgtttttttc gtttaatcct gtctaatgcc
     3361 gtcaaccaat cccaatcaat acaaggattt caggctgatt gttatcctgc cgtccccctt
     3421 cctgacagtg caatcccgtc caatccgccc taattgaact aacctgattt tttacagcat
     3481 ccgaaaatac cgcgagaaaa taccgtaaaa ataaagctga acgaccacca aatcaggaat
     3541 gccaagcggg aaatagcttg cggggaatac tgacgcaccg tagggaatga gggggaaacc
     3601 gtccaagatg cagagcggtt tttttttggg tttttggaaa aaacctatac tatagtggat
     3661 taaatttaaa ccggtacggc gttgcctcgc cttgccgtac cggtttaaat ttaattcact
     3721 ataaaataag aaaaagataa aaaattggta acaaatgcgg taacaaatgg taacgaatcg
     3781 gtaacaactt ttggggtttt ccggtttttc accgtcttgg cagtgggagc gtagcggaat
     3841 gaaaagccaa aacgcacgga accgcgccta ttttgagcag gaatggcggt taaaccgctt
     3901 ggttatatac ggggaatagg aagacagcga aacgcgctaa aaacgcaatt tgagccatta
     3961 aaagcgattg attaaaaaat aatcaggtta gccgccagtg tttcaggcgg cataaacgga
     4021 gaaattgcag ggtataaaaa ggcggcttgc cgtgttgtct gtctctggta taattccaag
     4081 tatcactaat caacggctac acaatgcgga tattcaaaaa ccaatggata gtgaaatttg
     4141 ccaagaaaca taaaatcagt gattccgagc tgctggaagc ggtagagcgg gcggataacg
     4201 ggctgataga cgcagattta ggcggcggtg tgattaagca gcgcatagca aggcaaggac
     4261 aaggcagaag cggcggctat cgcagcctga tattgttcaa acaggcagat agggcatttt
     4321 tcgtttacgc ctttgccaag aacgacaggg aaaacatttc ggataaagaa ctggacgttt
     4381 accgaaaagc cgccgcatat tatctgaaat acacgcgggc agagctggcg gctttgaaag
     4441 aagacggcat tatcacggag atagaatcat gaaatacaaa agcgaggcat tagccgccat
     4501 tcatgaaatg atggaagggg cttacaacat cggcgcaatc gacaaaaaga ctatgcgcgg
     4561 ctttgacaag tcctgcctga ccgaaatcaa accgttgagc ggcggagaca tcaaggcaat
     4621 cagggagaag gaggcactat cgcaagccgc tttcgccatc tatctcaacg tgggaaaaaa
     4681 tcacgtttcg gcttgggagc ggggcgttaa aaagccgagc ggcgcggcgt tgaagctgct
     4741 gaccatcgtc aaaaacaagg gtatcgaagc cattgcgtag ccgacttggc aaacggcaaa
     4801 atcagcaagt tcacaataga cacgctgctg aatatgcctg ccaaaacagg caagaccgcc
     4861 gaactgaata tcagggcgta gccgcataaa tgcccgaccg catcaaacca agccgaaacg
     4921 gcgacggtgc agacgacata gcccgacagc aaggcacggc gcagacgggc gcgaaacccg
     4981 aaacatcccc gaccgcgagg cacggggatt ttttgagccc gttgcagggg gattggattt
     5041 aagcggcgcg ggcttgaaag caaaacgggc ggggcgcaga actgtttaaa tgccgtctga
     5101 atctcaaacg atttcagacg gcattttgaa acaacggctc aaattctcga tccccttccc
     5161 ttaacgccga cgttttttat taacgcgccc cttatttctg acactttgct cataaaccgg
     5221 cataacggtc ggcaacaacc gttttagatt ttctatacgg gcattgttta cagcatcagc
     5281 ataagacacc gtactctgga ataccgccta aaccctgcgg atagttttgg atgtggtatc
     5341 gacattgtcg gcagacttgt ctaactcgac cgttttcaaa taatctttga cagatgctgc
     5401 attcagtgtc gcggaatccg cgtattttga acaaaaggtc gggctacttt atcacaatag
     5461 cagaaagatg gaaaacggcg gggattggtg aaaggtcgtc tgaaatgata ttcagacgac
     5521 cttgaacggc tgtgttttaa atatggtatt tattcatttg tttcaccatc aggcagtcca
     5581 aaccacctta agggaatcgt gttaaattgg taatggcagt taacactgat aatataatct
     5641 aaaacatctt tatattccgg ctttgtaaac caatcattca aaacatagac atattcaacc
     5701 cgccaaccta aagagtgaac caattttgta tattgttttc tcttaaaatc gcaagtttgt
     5761 aatttctcat ctactgaacc gggagtttgt tggaatttga tctcaataat aaataaggta
     5821 tcacggatga tgataaataa gccattgtcg ggtagtaatc ttttagacag atggtctttc
     5881 caattaatat tgtatcccgg tcttttcaaa aatcggtaaa actcatattt tttaaaacaa
     5941 taagccttca attctccatt gaaccaaact tcataacccg cattatcata tgtttttctt
     6001 aagtcgtacc cgggaatttc ttcaaacaac gttcgtaagt ctgtacgttc ttcgaatctg
     6061 agtcctgttt gtgtccttgc accaccgatt ccgccaataa tcatttttct aacctttttt
     6121 ttgctaattc aaaaaaatct tcttccagtt cgcaaccaca aaatttccga ccatgtttta
     6181 aggcggcaac gcctgttgtg ccgctgccca tgaaagggtc aaaaattaaa tctccgatgt
     6241 ttgaagctga gagtatgcag cgttcaagca aggggagtgg tttttgtgtc ggatgtttgc
     6301 cgaatgtttt ttcggctttg tttggaggag gaaatgtcca aacacatttc atctgtttgc
     6361 catcattttg tgctttcatc atttcataat gaaacgtgtg tttggctttt ttatttttct
     6421 ttgcccataa gattgtttct gtcgaatggg taaaaaaacg gcaggacaaa ttagggggag
     6481 gattgggttt ttcccaagta atattgttca aaatatggta tccgatagtt tgcatcaggt
     6541 agccgattaa ataaatatta tggaatgtgc cgcaaaccca aattgtgccg tttggtttta
     6601 ataaggcgta acacagtcgg agccattctt cgtagaattc caaatctgcc gccattcctt
     6661 tggatttatc ccagttgccc ttattgacgg aaaccatttg tccgttttga caactgaagc
     6721 cgtcattgga aagaaagtaa ggcgggtctg agaaaatcat atcaaaacag ccgtttggat
     6781 atttttccaa tatcttccgc atgacgttaa gggaattttc gttgtagagg acaaggtcgt
     6841 ctgaaacgat ttttttcaaa cgactttgat aaatggcgtc ttcttcagcc ggtttgtcaa
     6901 aatcaatcat aaattgccaa taattctccg acttttttac ggctgctgcc ttttgcggca
     6961 atattgcgtt gtacttcaat tgttttcaga atggcaaaag aataaatatc ccttgtgaat
     7021 tcagtatcat gatttgaaat caaaacgcct tgggaatcgg gcgcaatttg ttgggctgac
     7081 tgggcaagac ggatttgatt gtctaaattg aaactttctt tggcataagt ggtaaatgaa
     7141 gcggttgggc ttaaaggcgc gtaaggggga tcgcaataaa ccgtatcggt attgtctgtc
     7201 tgagatagaa tcatttgaaa atctccgcac attaattcaa ctctgtcaga tttctgaatg
     7261 aaaccttcca tttcttgttg gggaaagtac ggcgatttgt agcgtccgaa cggcacattg
     7321 aatacccctt tgctgttgta acggcataaa ccgttaaaag catgacggtt caaataaata
     7381 aatagtgctg agcgttcgtg cagatttttg ctgaagttga attgttcccg caattcatag
     7441 aatcttgaat cgctattgtt ttcggaaata aagaaagagc gggcgtagtc gatgaatccg
     7501 gatttttctt cttttaggat acggaataag ccgattaagt cagcgttaat atcattgagc
     7561 agataatgtt cgaagtccaa tgcaagagac aaagcggcag aaccgcaaaa gggttcaatc
     7621 aggcgttttc gtgtaggagt gggtagatta tgttcgataa aggggacaag tttatgtttg
     7681 ccgcctgccc attttaggaa gggttttgaa gccattagga tgcagagtat aagcgtggtt
     7741 gattggcttg gaattgtgcc aaaacatcgt gaggaataca acggatttat attttgggga
     7801 cgtgtgtaga gtgagtctaa atccaataga acctttccat tccaatatgt tattggactt
     7861 atcaactcca tctgccttaa aaaggccgtc tgaaaccctg tttcccagtt ttcagacggc
     7921 ctttatccgt atggctaaac cttaagagcg gtttagacga cgatattcac caagcgtccg
     7981 ggaacgacga tgattttctt cgccggtttg ccttccatga atttcaccgc gccttcggtg
     8041 gcgagtgcgg cggcttcgag gtcggctttg gatgcgtcgg cggcaacagt gattttgccg
     8101 cgcagtttgc cgttgacttg aaccatcact tcgatttcgg atttgaccaa ggcggcttcg
     8161 tcgactgtcg gccagcctgc ttcccacagt ttcgcgccgt tcaattcgct ccacagggtt
     8221 tcgcagatgt gcggcacgat gggccacaac aggcgtacgg cggtttccaa tacttcttgg
     8281 gcgacggcgc gtccttgttc gccgccggtg tcggttttgt cgtattggtt gagcaattcc
     8341 atcacggcgg cgatggcggt gttgaactgc tggcggcggc cgtagtcgtc gctgactttg
     8401 gcagtggtcg cgtgcagttt gtggcgcagg tctttgagtt ctttagacaa accgtcttgg
     8461 ctgcctgcga acgctttgac cgcttcgcct tgcttcaagt attcgtaaac ggtacgccac
     8521 aggcggcgca ggaagcggtg tgcgccttcg acgccgctgt cgctccattc gagggactgt
     8581 tcgggcggtg cggcgaacat cataaacagg cgggcggtgt ccgcgccgta ggcgttaatc
     8641 agttcttgcg gatcgacgcc gttgtttttg gacttggaca ttttttccgt gccgctgatg
     8701 acgacgggca gcccgtcggc tttgaggacg gcggaaatgg ggcggccttt gtcgtcgaac
     8761 gtcagctcga catcggcggg gttgatccaa tctttgccgc ctttgtcgtt ttcgcggtag
     8821 taggtttcgc aaacgaccat gccttgcgtc agcaggcgtt caaacggttc gtcaacattg
     8881 actagacctt cgtcgcgcat cagtttggtg aagaaacgcg cgtacaagag gtgcaaaatc
     8941 gcgtgttcga tgccgccgat gtattggtcg accgcgcccc agtatttcgc ggcggcagga
     9001 tcgaccatgc cgtctgaaaa ttttggcgac atatagcgga agaaatacca gctcgattcc
     9061 atgaaggtgt ccatggtgtc ggtttcgcgt tttgccgcgc cgccgcagca tgggcaggca
     9121 gtttcgtaaa actcgggcat ttttgccagc ggcgaaccca tgccgtcggg tacgacgttt
     9181 tcaggcaaaa cgaccggcaa ttggtcggca gggacgggta cgtcgccgca ttgttcgcaa
     9241 tggacgatgg gaatcgggca gccccagtag cgttggcgcg aaatgcccca gtcgcgcagg
     9301 cggtattggg ttttcggttc gcccgcgcct tggctttgca gcttggcggc aacggcatcg
     9361 aatgccgtct gaaaatccaa gccgtccaag tcgccgctgt tgaccaatac gccgttttct
     9421 ttgtcggcat accattcttg ccatcggttt gcgtcgaatg cgttgtcgcc gacggcaatg
     9481 acttgttttt taggcagatt gtatttggcg gcgaactcaa aatcgcgttc gtcgtgcgcc
     9541 ggaaccgcca tcactgcgcc gtcgccgtag ccccacaata catagttggc aatccacact
     9601 tccagcttgt cgccgttgag cgggttgacg acgtagcgac cggtcggcac gccttttttc
     9661 tccatcgtcg ccatatcggc ttcggcaacc gaaccggctt tgcattcggc aataaatgcc
     9721 tgcaattcgg gtttgtcggc ggctgcggcg gctgccagcg gatgctcggc ggcaacggca
     9781 acataagtcg cacccatcag cgtgtcgggg cgggtggtat aaacttgcag gaatttcgcg
     9841 taatcgcctt ccaagccttg tttgctgtcg tctgaaacgg cgaagcgcac ggtcataccg
     9901 cgcgatttgc cgatccagtt gcgctgcatg gttttgactt gttccggcca gtgttccagc
     9961 ttgtccaagt cgttgagcag ctcttcggcg taatccgtga ttttgaagta atacatcggg
    10021 atttcgcgtt tttcgatcaa cgcgcccgaa cgccagccgc gtccgtcgat gacttgctcg
    10081 ttggcaagga cggtttggtc gaccgggtcc cagttcaccg tgccgttttt gcgatagacg
    10141 atgccttttt caaacagctt ggtaaacagc cattgttccc agcggtagta ttcgggtttg
    10201 caggttgcgg tttcgcgcgc ccagtcaatc gcaaaaccca ggcttttgag ctgggttttc
    10261 atgtattcga tgttatcgta cgtccaagcg gcaggggcga cgttgttttt catcgccgcg
    10321 ttttccgccg gcatgccgaa cgcgtcccaa cccataggct gcatgacgtt gaagccgttt
    10381 aaaagtttga agcggctcaa tacatcgccg atggtgtagt tgcgcacatg ccccatgtgc
    10441 agcttgccgc tgggataggg gaacatggag aggcaataat atttgggttt ggaagcgtct
    10501 tcggagacgt tgaaaatacg ggcgtcgtcc cattttttct gcgccgcagg ctcgatggcg
    10561 gcgggttggt aatgttcttg catggtcatt ctgtttttcg cttgaaaacg ttaggaattt
    10621 aaaggaggaa ttataacagg ttgccgatgt cggcgaatcg gcaaattgcc ggcaggatgc
    10681 gtaaattcgc acgcgcatca ttccgtatgc cgtacaaata caccgcgttt actgatacgc
    10741 acgtttttta tgctaatatt acaaaccaaa atcaaatgtt taaaactctc ctgatgcggc
    10801 tcttccgaac aaaaggcaga cgggcatcgg gtaaaagagg attctgcata tgaaaatcaa
    10861 acaaatcgtc aaaccgggct tggcagtatt ggcggcgggc gttctgtctg cctgcgcaac
    10921 caaaagcaac gtcaaagccg acggaacgac cgacaatccg gttttcccga aaccctattc
    10981 cgtaacgctc gacaacaatc gcggtacatt cccgacctat gacgaattgg acttgatgcg
    11041 tcccggtctg accaaagacg acatctacaa aatcctgggt cgtccgcatt acgacgaagg
    11101 tatgtacggc gtgcgcgaat gggattatct gttccacttc cacaccccgg gcgtaggcat
    11161 cgaccctgaa aacacttccg gcgtagaagg cattaccacc tgtcaataca aaattatttt
    11221 cgataaagac aaatttgccc gcagcttcta ctggaacccc gtcttcccga aagatgccgc
    11281 ctgtccgccg cccgcaccca aagccgagcc gcaagtcatc atccgcgaaa tcgtgccggc
    11341 aaaaccgaaa cgtatccgcc aataatccga catgccgttc cgcctgtttt tagggatatt
    11401 atgcggcctg tcaatggttg cccccgtata tgcacagggg caaccggatg cggtcaacgc
    11461 ttatattcaa aagaaaaaag tcatcgtaaa cacatccaaa gcggaactct gttttgccga
    11521 tgacaggcag tgccaccccg tcctcatcgg tgttgccacg cccaagggga cgttcgggct
    11581 gacgctgaac agtaccgaca agcctggata cggcggcgaa gtcatcggtt tcaagcagga
    11641 gggtgatttt cttttcgccc tgcaccgcgt ttggaatcag ataccgtcgg aaaggcggaa
    11701 cgaacgcatc gcctccccgt ccgtgtccga caggattatg accaacggct gcatcaacgt
    11761 cagcgatgcg gtgtacgaaa aactgcgtca ttattttgtg ttggaagtga tttgaaacag
    11821 acggataccg cacgcgccgg tatctgtttt cacattgccc cgatgcctga aacagactgt
    11881 ccgccacgtc atgccgtctg aaaccggcgc agatgccgcc aagccttcag acggcattgc
    11941 ctgcccgctc cgaccgaaca acaaccatct ttgggagaac cttatgcccg aacaaaaccg
    12001 catcctctgc cgcgaactaa gcctgctggc attcaaccgc cgcgtgttgg cacaggcgga
    12061 agacaaaaac gtcccccttt tggaacgcct gcgcttcctg tgcatcgtgt cgtccaatct
    12121 tgacgagttt ttcgaagtcc gcatggcgtg gctcaagcgt gaaaacaagc tgcacccccg
    12181 gcgcaggctg gacaacggca aaatgccgtc tgaaaccatc gccgacgtta ccgaagcggc
    12241 gcgctccctg atacggcatc agtacgacct gttcaacaac gtccttcagc ccgagttggc
    12301 gcgggaaagc atccattttt accgccgccg caactggacg ggcacgcaga aaaaatggat
    12361 cgaagactat ttcgaccgcg aactgctgcc gatcctgacc cccatcggac tcgacccgtc
    12421 ccaccccttc ccgcgaccgc tgaacaaatc gctcaacttc gccgtcgaac tcgacggcac
    12481 ggacgcattc ggcaggcctt cggggatggc gattgtgcag gcaccgcgca tcctgccgcg
    12541 cgtcgttccc ctgccgtccg aactgtgtgg cggcggacac ggcttcgtct tcctgtcgtc
    12601 catcctccac gcccacgtcg gcaaactctt cccgggcatg aacgtcaaag gctgccacca
    12661 gttccgcctg acgcgcgaca gcgacttgac cgttgacgaa gaagacctgc aaaacctccg
    12721 cgccgccatc caaaacgaac tgcacgaccg cgaatacggc gacggcgtgc ggctcgaagt
    12781 cgccgacacc tgtcccgcct acatccgcga ctttctgctc gcgcaattca aactgaccgc
    12841 cgccgaactc tatcaggtca aaggcccggt caacctcgtg cgcctcaacg ccgtccccga
    12901 cctagtcaac cgccccgatt tgaaatttcc cacacacacg ccgggcagac tgaaagcctt
    12961 gggcaaaacc gcgtccatat tcgatttggt gcgccaatcg cccatcctgc tgcaccaccc
    13021 ctaccaatcg ttcgaccccg ttgtcgaaat gatgcgcgaa gctgccgccg accccgacgt
    13081 gcttgccgtc aaaatgacga tttaccgcac cggcacgcgt tccgaactcg tccgcgccct
    13141 gatgaaggcg gcactcgccg gcaaacaagt aaccgtcgtc gtcgaactga tggcgcgttt
    13201 tgacgaagcc aacaacgtca actgggcgaa gcagctcgaa gaggcgggcg cgcacgtcgt
    13261 gtacggcgtg ttcggctaca aagtccacgc caagatggca ctggtcatcc gccgcgaaga
    13321 cggcgtactc aagcgttacg cccacctcgg cacgggtaac taccaccaag gcacatcgcg
    13381 catctacacc gacttcggca tcattaccgc cgacgaacaa atcaccgccg atgtgaacac
    13441 attgtttatg gaaatcacag gcttgggcaa acccgggcgg ctgaacaaac tctaccaaag
    13501 cccgtttacc ctgcacaaaa tggttatcga ccgcatcgca cgcgaaaccg aacacgcaaa
    13561 agccggcaaa ccggcgcgga ttaccgccaa gatgaattcg ctcatcgaac cgaccgtcat
    13621 cgaagccctg tatcgggcaa gcgcggcagg cgtacaaatc gatttgattg tgcgcggtat
    13681 gtgcaccttg cgcccgggtg taaaaggctt gtccgaaaac atccgcgtcc gctccatcat
    13741 cggcaggcag ctcgaacacg cgcgcgtgta ttgcttccac aacaacggcg cggacgatac
    13801 ctttatctcc agcgcggatt ggatggggcg caacttcttc cgccgcatcg aaaccgccgc
    13861 gccgattgcc gcgcccgaac tcaaaaaacg cgtcatccgc gaaggtttgg aaatggcact
    13921 ggcagacaat acgcacgcgt ggctgatgca gcccgacggc ggctatatcc gcgccgcgcc
    13981 tgccgagggc gaaagcgaag ccgacctgca aaacgatttg tggacactgc tcggaggctg
    14041 acccgcaccg ccccaatcaa aaaccatgcc gtctgaaact tttccgtttc agacggcatg
    14101 gttttacagc aatctaaaca gggcggactg gaatcaaaaa cacaccttcg ccattcccgc
    14161 acaagcactt cccctatacg ctcccaaccc caatccgccg cattccagac ggcattgcct
    14221 ttccgacagc cgaacaaaac cccgaatccg tctgcacggt tcggggcata tctccaatac
    14281 gggcatcgtg ctccggaaaa ccgtcaaatc cgcatcggca tcacaatata tttgaaattc
    14341 ggattgttcg gcacggtaaa cagcgtcgag cggttggcat cgccgaaggc aagctgcata
    14401 tcgtcggaat ggatgttgcg caacacgtcc atcagatagc cgatattgaa accgacttcg
    14461 agttcgccgc cctgataggc aatctcgatt tcttcgcgcg cttcttcctg ctcgttgttg
    14521 ctgcacacga cgctcaacag gccggattgc aggaacaggc gcgcgccacg gaatttttcg
    14581 ttggcaagaa tggacacacg ttccagcgcg cccaaaagtt cggcacggga caaaacgaaa
    14641 atcttgtcgt tgtccaaagg gatgacgcgg ttgaaatcgg ggaatttgcc gtcgataacc
    14701 ttgctgacga tggtcgtgcc gttgcattgg aaacgcacct gcttgtccag cagctcgatt
    14761 tgaatcggat cgtcggggtt gttcaacagt ttgaacagtt ccagcaccgt tttgcgcggc
    14821 aaaatcactt cggcgcgcgg caaatccgca tcaatcgcgc aggctgcata ggcaaggcgg
    14881 tgtccgtcgg tcgccacaag gcgcaactgg ctgccctcaa cctgcatcag cagaccgttg
    14941 agataatagc ggatgtcctg caccgccatg ctgtactgca cttgcgacag catggttttg
    15001 aaacgctcct gctccagcga gaaagtcgcg ctgatgtcct cgccgacatt catcatcgga
    15061 aaatcggcgg caggcagggt ctgcagggca aaacgcgatt tgcccgcctt cagcgtcaga
    15121 cggctgtcgt cccaatccag cgacaccagc gcaccggcag gcagcgcgcg caaaatgtcc
    15181 tgaaatttct tggcattggt ggtgatgcgg aagtcgtccg cgccgccctc gggacccgca
    15241 gtgtcgattt ggatttccaa atcggttgcc aagagtttgg tctgaccgcc ttttccctca
    15301 atcaggacgt tggacaggat gggcagggtg tggcggcgtt cgacgatgcc ggtaacggct
    15361 tgcagcggct tgagcaggct gtcgcgttcg gcttgtaaaa tcaacatgtt cgctccttta
    15421 aatcggttgg attttttacc cttcggacgg catgggcaat atcatgccgt ctgaaaacgt
    15481 tttccatcag ttttgaatca gaatcagcag cttttcataa tcctgagcca attccggatc
    15541 ttcttcgcgc agtttcgcca ctgccctgat gccgtgcata acggtcgtat ggtcgcgccc
    15601 accgaacgaa tcgccgatag acggcaggct caaagtggtc agttctttgg tcaggctcat
    15661 cgccacctgg cgcggacggg caatgttgcg cgtgcgtttt ttgccgagta cgtcgctgat
    15721 tttgatgcgg taatatttgg caacggcatc aataatgata tcggcggtaa tgaccttatg
    15781 cttctcggca ataatgtcct gcaaagcggt acgcgccaaa tcgatgtcga tgacgggacg
    15841 gttcataaag cggctgctcg ctccgacacg gttaaacgcg ccttcaagct cgcgcacgtt
    15901 ggaacggatc agattggcaa tgaacagcgc ggcttcgtct tcaatactga tgcccgccgc
    15961 ttccgccttt ttctgcaaaa tggcgatgcg catttccaat tcgggcggct cgagttccaa
    16021 agtcagtccc catgaaaaac gggatttgag gcggtcgtcc atgccttcga ttttggcggg
    16081 taaaacatcg caagtgagga tcagctgttt tttctcgttg tggaaatggt tgtacagata
    16141 gaaaaactct tccatcgtac ggtcttttcc tttaataaac tgaatatcgt cgataatcag
    16201 caggtcgtat tgcttgtatt gctgcttgaa tacgtcatag gtattgttgc gaaccgcctt
    16261 cataaagctg cggatatagt cgtccgaatg catatagcgc actttggcat cgggacggtt
    16321 tttcaaaagc tcgttgccga ccgcctgtac caggtgggtt ttgcccaaac ccgtgctgcc
    16381 gtacaggaaa aacgggttgt agctctgccc cgggctttcc gcaatcgcct gcgccgcagc
    16441 cgccgcaagg cggttgccct tgccttcgac caatgtgtcg aacgtatagt ccggcgacag
    16501 attggtctgc tcgtaacgtg cctcttccgc atcgcgctgc gcatccgtcc gcgctttcgc
    16561 aagcacctcc gattccggac gggaagcagg ttcggcagta tggcgcggct catgcgggag
    16621 gtttttcata cgttccgcca gaatatccgc cgccgttttc gacgcagcgg gtttgacagg
    16681 ctcttcagac ggcagctcgt ccaacagaac ctcctgcaca ggcatctcct ccgaccccgc
    16741 gtgcaaggac ggctcggcag gttcgacagc accttcaacc gccgccatct cataacgcac
    16801 gccttctcct ggtttgaata cgaaggcgga acggccagca gccaactctt ccctcaccgc
    16861 ttctattttc ccggcaaact ggctcttaag catattgcag gcaaactggt tcttgccgta
    16921 caccacccat acgccaccct cctcaccgac ggtaaggggc gcaatccatt gcgcaaactg
    16981 cccttgaggc aacatatcgt gaagacggcg gaggcacagc ggccaaaact ctgctaatgt
    17041 catggataag ctcgaatcgg taaaaatgaa atcgaaaaca aagaaaatat aatattttca
    17101 aaaagaaaac aaatctgttg aacacacatc ggttcaaaat gcagcgaacc gaattatacc
    17161 gactcgccga tattttatcc acaacccgtg caaaatttat ccacagaaag gcggcagaaa
    17221 tccgcaggca atcgggcaat cctcctgcaa agtttctata ttgattgaca aaagcggcaa
    17281 attggagtgt aattcacggt ttaattatct acccattcta tttttaggaa acatcatgaa
    17341 acgcacttat caaccttccg ttaccaaacg caaacgcacc cacggcttcc tggtgcgctc
    17401 caaaacacgc ggcggccgcg cagtattggc tgcacgccgc gccaaaggcc gcaaacgcct
    17461 ggcggtataa ttttggacta ccgcttcgga aggcagtacc gcttgttgaa aacggatgat
    17521 ttttcatccg tttttgcatt cagaaaccgc cgcagccgcg acctgctgca agtttcgcgt
    17581 tcaaacggca acgggctgga ccatccccgc atcggtctgg tggtcggcaa aaaaaccgcc
    17641 aaacgcgcca acgaacgaaa ttatatgaag cgcgtcatcc gcgactggtt cagattgaac
    17701 aaaaaccggc tgccgccgca ggatttcgtc gtgcgcgtcc accgtaaatt cgacaggtct
    17761 accgcaaaac aggcaagggc ggaactggca caactcatgt tcggcaaccc cgcaaccgga
    17821 tgcaggaaac aggcatgatc agaacggtac tctgcaggca aggttcagac agcaacgggt
    17881 ttcccataca aggaacatcc cgatgaactt cctattgtcc aaactcctgc tgggactgat
    17941 acggttctac caatattgca tcagcccgct gattccgccg cgctgccgct atacgccgac
    18001 ctgttcgcaa tacgccgtcg aagcagtcaa aaaatacggc gcattcaaag gcggccggct
    18061 cgccatcaag cgcattgcac gctgccaccc cctcggcgga cacggacacg accccgttcc
    18121 ctgacccgac gcaatattca aattgcacgc tttcctttta tttcccatcg gtttctatat
    18181 aatgccgtct gaagcttcgg gcaggcggca cgaccgtcgg gtatgaaacc cgcccttatt
    18241 ccccgtctat cggaacacgc aacctgcggc atttccgacc attcaggaaa ctcttatgga
    18301 ttttaaaaga ctcacggcgt ttttcgccat cgcgctggtg attatgatcg gctgggaaaa
    18361 gatgttcccc actccgaagc ccgtccccgc gccccaacag gcagcacaac aacaggccgt
    18421 aaccgcttcc gccgaagccg cgctcgcgcc cgcaacgccg attaccgtaa cgaccgacac
    18481 ggttcaagcc gtcattgatg aaaaaagcgg cgacctgcgc cggctgaccc tgctcaaata
    18541 caaagcaacc ggcgacgaaa ataaaccgtt catcctgttt ggcgacggca aagaatacac
    18601 ctacgtcgcc caatccgaac ttttggacgc gcagggcaac aacattctaa aaggcatcgg
    18661 ctttagcgca ccgaaaaaac agtacagctt ggaaggcgac aaagttgaag tccgcctgag
    18721 cgcgcctgaa acacgcggtc tgaaaatcga caaagtttat actttcacca aaggcagcta
    18781 tctggtcaac gtccgcttcg acatcgccaa cggcagcggt caaaccgcca acctgagcgc
    18841 ggactaccgc atcgtccgcg accacagcga acccgagggt caaggttact ttacccactc
    18901 ttacgtcggc cctgttgttt atacccctga aggcaacttc caaaaagtca gcttttccga
    18961 cttggacgac gatgccaaat ccggcaaatc cgaggccgaa tacatccgca aaaccccgac
    19021 cggctggctc ggcatgattg aacaccactt catgtccacc tggattctcc aacctaaagg
    19081 cagacaaagc gtttgcgccg caggcgagtg caacatcgac atcaaacgcc gcaacgacaa
    19141 gctgtacagc accagcgtca gcgtgccttt agccgccatc caaaacggcg cgaaagccga
    19201 agcctccatc aacctctacg ccggcccgca gaccacatcc gtcatcgcaa acatcgccga
    19261 caacctgcaa ctggccaaag actacggcaa agtacactgg ttcgcctccc cgctcttctg
    19321 gctcctgaac caactgcaca acatcatcgg caactggggc tgggcgatta tcgttttaac
    19381 catcatcgtc aaagccgtac tgtatccatt gaccaacgcc tcttaccgct ctatggcgaa
    19441 aatgcgtgcc gccgcaccca aactgcaagc catcaaagag aaatacggcg acgaccgtat
    19501 ggcgcaacaa caggcgatga tgcagcttta cacagacgag aaaatcaacc cgctgggcgg
    19561 ctgcctgcct atgctgttgc aaatccccgt cttcatcgga ttgtattggg cattgttcgc
    19621 ctccgtagaa ttgcgccagg caccttggct gggttggatt accgacctca gccgcgccga
    19681 cccctactac atcctgccca tcattatggc ggcaacgatg ttcgcccaaa cttatctgaa
    19741 cccgccgccg accgacccga tgcaggcgaa aatgatgaaa atcatgccgt tggttttctc
    19801 cgtcatgttc ttcttcttcc ctgccggtct ggtattgtac tgggtagtca acaacctcct
    19861 gaccatcgcc cagcaatggc acatcaaccg cagcatcgaa aaacaacgcg cccaaggcga
    19921 agtcgtttcc taaatgccgc agcatgaaaa atgccgtctg aaacctgttc agacggcatt
    19981 tttattgccc accccctatc ggggcggaaa tcttcaaccc gcatacatca caaaaatcgt
    20041 cgggcgtttt ttcagattgg gcatttcttt tctttttcgc cactgcacga ttgtttgact
    20101 gatgatttcc tgtgtcggca aggtcaaatc cgtagccgtg cataaacgcg tttcaggatg
    20161 caggttttcc accgcatcgg caagcagcgc atcattgcgg taaggcgttt caataaaaat
    20221 ctgcgtctcg ccgcactggc gcgaacgctg ttccaaagcc cgaaaagcct gaatccgctc
    20281 gtttttttca gacggcagat agcctttaaa cgcaaaactc tgcccgttcg cacccgaagc
    20341 catcaaagcc agcagcaggc tggaaggccc gaccagcgga cgcacttcaa aaccgtgttt
    20401 atgcgccaat gccaccaaat tcgcacccgg atcggccaca gccgggcaac ccgcctcact
    20461 gacaatgccc atactgcgcc cttcttgcaa aggtttcagc aattccggca aagtcttcaa
    20521 atccgtatgt tcattcaacg tttgcagatt cagctcgcgg ataggcgtag tcacgcccaa
    20581 atgtttcaaa tgcgcacgcg ccgttttttc cgcctccacg acaaaatccg tcagcccgac
    20641 aatcgcctgt tgttcatgcg gcaacaggca cggcgtgtca ggcgtaccca aaggcgtagg
    20701 aatcaaatac aaaacaggag acatcattcc ctcactcatc ggttaaaaat gccgtctgag
    20761 cctttcagac ggcataaacg ggcagttaca gaacctccac gccctcattt ttcaagaaat
    20821 cgaccagacg gaaaaccggc aaaccgatta aagcattcgg atcggtactc tcaatccttt
    20881 caatcagcaa tgcacccaaa tcctcactct tcagcgcaca cgaacaataa accgcatcag
    20941 gctcgcgctc caaatagcgg aggatatgca actcgtccaa ctgcctcatc acgaccaccg
    21001 tcttatcgat atgccgccgc atcctgcccg taaccgtatt caacagcacg atcgcgctgt
    21061 aaaactcaat ctccctgccg ctcaagtgca tcagcatctt ttgcgcattg gcaaggttca
    21121 tcggcttgcc ccactgcctg ccgtcgcacc acgccacctg gtccgcaccg acaatcaacg
    21181 cctctgggaa acgcccggtc aacgaccgcg ccttaccctc ggcaaggcgc aatgccgtct
    21241 gaggggcgga ttcccccaac atcggcgttt cgtcaaaatc gggggacgcc gcctgaaagg
    21301 caatgccgag cctttccatc tgttcgcggc ggaaaaccga actcgtaccc aaaatcaaag
    21361 gcagttccaa acccatccca tcctccttac cgttgaaaac acgcccgaag gggcagtaaa
    21421 atccagccat gcgccgaaac acggataccc gccttcggcg taccgcaaca tttttcttaa
    21481 aaatattgac gttagaacat ctaaattata tcatatcccg tttatgtcag accctaattt
    21541 gattgacttg gaaatttttg ccgccgaagg gcagaacctg caaggcagtt ttctgctgga
    21601 agaattggat gaacgcgtca gttcgcacga ttatcccgcc gacaggcaga ccaaaatatc
    21661 gtttacactg accggcggtc gcgaccggct gcaacgcctg ttcctcgacc tgaacgtcaa
    21721 agccgatatg cccctgattt gccagagatg tatcaaaccc atgccgttca tgcttgatga
    21781 aagcagccgt atcgtcctgt tttccaacga agagtccttg gacgaatcca tgcttgccga
    21841 cgaagaactc gaaggcatac tgattgaaaa agaactcgac gtgcgcacat tggtagaaga
    21901 ccaaatcctg atgtccctgc ccttttcgcc gcgacacgaa gactgcggcg acaatgggac
    21961 actggaagaa gtcaatcggg acaaacccaa cccctttgct gttttggcag gtttgaaaag
    22021 caattgatta ggacacagtt tatttatcta ggagcttgaa atggccgttc aacaaaacaa
    22081 aaaatcccct tccaaacgcg gtatgcaccg ttcgcacgac gcgctgaccg cgcctgcact
    22141 gtctgtcgac agcacaaccg gcgaagtaca ccgcccgcac cacatctccc ccaacggtat
    22201 gtaccgcggc cgcaaagtgg tcaaagccaa aggcgaataa tccctattcg actgactgaa
    22261 aaagccagaa cattgccatg caattactgg ctttttttgc attggacgca ccatccgtcc
    22321 aaactttcgc catacgtcaa cacacagggg caaagcgttc cgtataatac cccgtgaaaa
    22381 tattccaaaa gccccaacca ccaaggaaat tccgatgaaa cagaaaatct ggtacaccta
    22441 cgatgacatc caccgcgtca tcaaagcatt ggcagaaaaa atccggaacg ccgacatcaa
    22501 atacgatgcc atgattgcca tcggcggcgg cggctttatt ccggcacgta tgctgcgctg
    22561 ttttctggaa attccgattt atgccgtaac caccgcctat tacgacagcg acaacgaagg
    22621 acaggttacc gaagaagtca aaaaagtcca atggctagac cccgtccccg aagccttacg
    22681 gggtaaaaac gtccttgttg tcgacgaagt ggacgacagc cgcgtaacca tggaattttg
    22741 cctgacagag ctgctcaaag aagacttcgg caccatcggc gtcgccgtac tgcacgaaaa
    22801 aatcaaagcc aaagcaggta aaatccccga aggcattccc tatttcagcg gcatcaccgt
    22861 agaagactgg tggatcaact atccgtggga cgcactcgac atcgacgaac acaaccgcct
    22921 tgccgaggcc ggccgaggct gaccctttca gacggcatat tttccgaacc gatgccgtct
    22981 gaagcccgca cgacccctgc cgcagaccga aaacctaccg gagaacccta tgattacact
    23041 tgccgtagat gccatgggcg gcgaccaagg acttgccgtt accgtacccg gcgcaacagc
    23101 attcctccaa gcacaccccg atgtccgcct gattatgacc ggcgacgaaa cgcaactgcg
    23161 ccaagccctg accgcggcag gcgcaccgat ggaacgcatc gacatctgcc ataccaccca
    23221 agttgtcggc atggatgaat cccctcagtc cgccctaaag aacaaaaaag actcctccat
    23281 gcgcgttgcc atcaaccaag ttaaagaagg caaagcccaa gccgccgtat ccgcaggcaa
    23341 cacgggcgca ctcatggcaa ccgcacgttt cgtcctcaag accatacccg gtatcgaacg
    23401 ccccgccatc gccaaattcc tcccgtccga caccgaccat gttaccctcg ccctcgacct
    23461 tggcgcgaac gtcgactgca cgcccgaaca gctcgcccaa tttgccgtta tcggcagcga
    23521 actcgtccac gcactccatc ctcaaaaagg acagccgcgc gtcgggctgg tcaacgtcgg
    23581 cacggaagac atcaaaggta cggacaccgt caaacaaacc tacaaactgc tgcaaaacag
    23641 caaactcaac tttatcggca acatcgaaag caacggcatc ctgtacggcg aagcagatgt
    23701 cgtcgtcgcc gacggctttg tcggcaacgt catgctcaaa accatcgaag gcgcggtcaa
    23761 attcatgagc ggagccatcc gccgcgaatt ccaaagcaac ctgttcaaca aacttgccgc
    23821 cgttgccgcc ctacccgccc tcaaagggct gaaaaacaaa ctcgatccgc gcaaattcaa
    23881 cggggccatc ctgctcgggc tgcgcggcat cgtcatcaaa agccacggcg gcacagacga
    23941 aaccggtttc cgctacgcac tcgaagaagc ctaccacgaa gccaagtccg ccggcctttc
    24001 caaaatcgaa caaggcgttg ccgaacaact cgccgcactc gaaactgcca aagccgtcca
    24061 aaacgaaaat gccgacggtc tgtaacacac acgatgccgt ctgaacgccc ccgccccttt
    24121 cagacggcat ccgcccgcac caaacctgcg ggcgcggacg gcgatgcgcc tgtccggcac
    24181 ttcccaaata cttccctgta aaataaggag tatttgaaaa atgaagacat tagaaaaacg
    24241 gatgaaagct ctagacaaac agatgatgaa gttcggaaaa tcccttgaag gcaggcttga
    24301 tgcccgtctg attgaatccg cattggatta tattcattat tcggaacgtt ttttggcttt
    24361 tgaaatcctg tgtacttata tcgaagattt cgatgtccgg ctgacggaac aagaatcccg
    24421 ggaaatttct tttatcaaca aggaatttga gatagaaagc acgtccgatt aaccaataaa
    24481 gccaatgggt tgataaacat gaaaacatcg acggtcgttt ttggcggatt ttttatggca
    24541 gacaacggag agcgaatcca aatccccgtt ttggaaaatc ctgacattag ggaaatcaat
    24601 cacttttttt ccgtatcaaa ttttgagaaa aaaaccggcg tccttgtttt cagaatcatc
    24661 cccgagccgg aatttggcaa taccgaatta actgtctatt ttgaaaaagg atattatttg
    24721 ccaatgattc aaacaatttt agaaggcggg gatattgaag tcaaaaacct taaaacggaa
    24781 aattatagtg gaaatacaat ggaaatttgg ggagattcct accctattga gcatatctct
    24841 aaaaatattt ctacaattca aaatataatt tctgaattta tgatacaaaa acaaacaccg
    24901 gcaccaataa tttgatggaa aaccaacaat caaaatgccg tctgaagcat gtttggtttt
    24961 cagacggcat ttccttcgct taaaacagcg tatcggcaac cccgccctgc ctgtccacgg
    25021 caatctgcat ctgaaaacca tctgtatccc aaaccacacc cccatccctg tttccgtcat
    25081 ttgcaccctg cccgtattgg gcaatcatcg gtttttcgct tacaatagcc gaatctgaac
    25141 caactatcca aaaaggccgt tcccatgcag tatgccaaaa tttccggcac gggcagctat
    25201 cttcccgcca accgcgtcag caatgacgac cttgcccaaa aagtggatac ctcggacgag
    25261 tggattaccg cgcgtacggg catcaagttc cgccatattg cagccgaaaa tgagaaaacc
    25321 agcgatcttg ccgccgaagc agcccgccgc gcactggatg cagccggatt agacagcggc
    25381 gaaatcgatt tgattatcgt ggcgacggca acgccggata tgcagtttcc ttctaccgcc
    25441 accatcgtgc agcaaaaatt gggcatcacc aacggctgtc ccgcgtttga cgtacaggca
    25501 gtgtgcgcag gctttatgta cgcgctgacc actgcaaacg cctacatcaa aagcggcatg
    25561 gcaaaaaacg cgctggtcat cggcgcggaa accttcagtc gcattgtaga ctggaacgac
    25621 cgcacaacct gcgtattgtt cggcgacggc gcgggcgcgg tggttttgag cgcggcggac
    25681 aaaccgggca tcatccacag caaactcaaa gccgacggca attatctgaa actcttaaac
    25741 gtccccgggc aaatcgcctg cggcaaagtt tccggttcgc cgtacatttc gatggacggt
    25801 cccggcgtgt tcaagtttgc cgtcaaaatg ctgtccaaaa tcgccgatga cgttatcgaa
    25861 gaagccggtt acaccgccgc tcaaatcgac tggatcgtgc cgcatcaggc aaaccgccgc
    25921 attatcgaat cgaccgcgaa acatttgggg ctgagtatgg acaaagtcgt cctgaccgtc
    25981 caagaccacg gcaacacgtc cgccgcctcg attccgctgg ctttggatac aggcatccgc
    26041 agcggacaaa tcaaacgcgg tcaaaacctg ctgctcgaag gcatcggcgg tggtttcgcg
    26101 tggggcgcgg tgctgttgca atattgaacc cgatgccgtc tgaagcaggt tttcagacgg
    26161 catttcccat atcatgaagc ggcaggcttt cttcaaacca attgcgtgtg cggcatttct
    26221 gtctgccgtt tcgctgcgcc tccccgtatt gggcgcgtgt tacgcaatat tgtccctcta
    26281 tgcgtttgca ctttacagca tcgacaaacg gcgtgccgtg cggggaaaac gccgcattcc
    26341 cgaacaccgc ctgctgctgc ctgccttgtt cggcggttgg gcgggcgcat acttgggcag
    26401 caggatattc aggcataaaa cggcgaaaaa gcgttttgtt gtgctgttcc gtctgactgt
    26461 ttcaggtaat gtcctggcga ccctcatcct g
//
LOCUS       DAWKST010000007        26431 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.007, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000007 DAWKST010000000
VERSION     DAWKST010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26431
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.007"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            182..538
                     /locus_tag="WJL87_000192"
     CDS             182..538
                     /locus_tag="WJL87_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ7798685.1"
                     /translation="MLSAYFPVFVFILIGLAAGVLFILLGTILGSKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFIYEWKKGALEWE"
     gene            529..1011
                     /locus_tag="WJL87_000193"
     CDS             529..1011
                     /locus_tag="WJL87_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ7798686.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            1024..1617
                     /locus_tag="WJL87_000194"
     CDS             1024..1617
                     /locus_tag="WJL87_000194"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ7798687.1"
                     /translation="MASIQNLYETVVGVLGDQASKVISALGEITVECLPEHYVSVMTT
                     LRDHEELHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVQNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            1607..2863
                     /gene="nuoD"
                     /locus_tag="WJL87_000195"
     CDS             1607..2863
                     /gene="nuoD"
                     /locus_tag="WJL87_000195"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ7798688.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELEGETIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYEGSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            2863..3336
                     /gene="nuoE"
                     /locus_tag="WJL87_000196"
     CDS             2863..3336
                     /gene="nuoE"
                     /locus_tag="WJL87_000196"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ7798689.1"
                     /translation="MLSAESLKQIDTELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            3726..5027
                     /gene="nuoF"
                     /locus_tag="WJL87_000197"
     CDS             3726..5027
                     /gene="nuoF"
                     /locus_tag="WJL87_000197"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEZ7798690.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVSFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEILKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIIMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGRMEDLDLLDSIGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            5027..5464
                     /locus_tag="WJL87_000198"
     CDS             5027..5464
                     /locus_tag="WJL87_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798691.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINKL"
     gene            5752..8013
                     /locus_tag="WJL87_000199"
     CDS             5752..8013
                     /locus_tag="WJL87_000199"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ7798692.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVNVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIIQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGVWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGIKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELLMPLLSQEAAHPDEWAGRLKNLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELAAATGAVLGILPQAANSVGADVLGVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTADGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLQGFEYHDTAAI
                     LKDALDVESLPSKLDNRNTWTGEGVQTTSDRLVRVGGVGIYHTDSIVRRSAPLQETGH
                     AAVPAARVNPNTLARLGLQNGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            8016..9092
                     /gene="nuoH"
                     /locus_tag="WJL87_000200"
     CDS             8016..9092
                     /gene="nuoH"
                     /locus_tag="WJL87_000200"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ7798693.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVILGVWMISPLNLWK"
     gene            9173..9652
                     /gene="nuoI"
                     /locus_tag="WJL87_000201"
     CDS             9173..9652
                     /gene="nuoI"
                     /locus_tag="WJL87_000201"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ7798694.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            9664..10335
                     /locus_tag="WJL87_000202"
     CDS             9664..10335
                     /locus_tag="WJL87_000202"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ7798695.1"
                     /translation="MTFSAILFYILAAIVLYGALRTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTVNPKRMDPADQVKVRADQGRMRLVKMEAVKPQTESAEESEVSDDLKPK
                     EEGKA"
     gene            10332..10637
                     /gene="nuoK"
                     /locus_tag="WJL87_000203"
     CDS             10332..10637
                     /gene="nuoK"
                     /locus_tag="WJL87_000203"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ7798696.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            10671..11246
                     /locus_tag="WJL87_000204"
     CDS             10671..11246
                     /locus_tag="WJL87_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine monophosphate-protein transferase
                     NmFic"
                     /protein_id="HEZ7798697.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKVVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            11370..11711
                     /locus_tag="WJL87_000205"
     CDS             11370..11711
                     /locus_tag="WJL87_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798698.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVSPK"
     gene            11760..13784
                     /gene="nuoL"
                     /locus_tag="WJL87_000206"
     CDS             11760..13784
                     /gene="nuoL"
                     /locus_tag="WJL87_000206"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ7798699.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFIDGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKIGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQTATIQ
                     LFPSVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHSDGHGEEHHGLGKNDNPHESPLFVTLPLILLAIPSVIIGYVAI
                     GPMLYGDFFKDVIFVNTDAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQAFRPVYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            13873..15369
                     /locus_tag="WJL87_000207"
     CDS             13873..15369
                     /locus_tag="WJL87_000207"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ7798700.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLATGSDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIYGAIHNPHVAEM
                     KDINCREFAILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            15379..16824
                     /gene="nuoN"
                     /locus_tag="WJL87_000208"
     CDS             15379..16824
                     /gene="nuoN"
                     /locus_tag="WJL87_000208"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ7798701.1"
                     /translation="MNWSDLNLMPALPEIALLSLLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSTGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKVAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGHVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRARPTVGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            16936..17235
                     /locus_tag="WJL87_000209"
     CDS             16936..17235
                     /locus_tag="WJL87_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ7798702.1"
                     /translation="MTASMYILLLLALIFANAPFLTTRLFGIVPLKRKHFGHHLIELA
                     AGFALTAVLAYILESRAGAVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(17344..18240)
                     /locus_tag="WJL87_000210"
     CDS             complement(17344..18240)
                     /locus_tag="WJL87_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ7798703.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSENEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGEAVHEAVEQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIQYDEATALLVGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(18224..18448)
                     /locus_tag="WJL87_000211"
     CDS             complement(18224..18448)
                     /locus_tag="WJL87_000211"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ7798704.1"
                     /translation="MKKNAPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGLKELNLESDE"
     gene            complement(18509..18685)
                     /locus_tag="WJL87_000212"
     CDS             complement(18509..18685)
                     /locus_tag="WJL87_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII"
                     /protein_id="HEZ7798705.1"
                     /translation="MPSEAGFGFQTASVFQKCYNPLFTGTSETMFQHAGRHLKHRPMC
                     RPDLEGLTPLPNKV"
     gene            complement(18712..19635)
                     /gene="rsgA"
                     /locus_tag="WJL87_000213"
     CDS             complement(18712..19635)
                     /gene="rsgA"
                     /locus_tag="WJL87_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ7798706.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIRAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETGAASPERLAFLQGITDELPG"
     gene            complement(19640..21496)
                     /locus_tag="WJL87_000214"
     CDS             complement(19640..21496)
                     /locus_tag="WJL87_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7798707.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVAWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESAQLFENIGT
                     VGDGMATLSKPHTILDKPNALPLKVSQGAIKFEHIDFCYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGITQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPDLSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            complement(21522..21659)
                     /locus_tag="WJL87_000215"
     CDS             complement(21522..21659)
                     /locus_tag="WJL87_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798708.1"
                     /translation="MPSEGGAAFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            complement(21659..22243)
                     /gene="ruvA"
                     /locus_tag="WJL87_000216"
     CDS             complement(21659..22243)
                     /gene="ruvA"
                     /locus_tag="WJL87_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ7798709.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            complement(22325..22651)
                     /locus_tag="WJL87_000217"
     CDS             complement(22325..22651)
                     /locus_tag="WJL87_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ7798710.1"
                     /translation="MNFKRLLLTAAATALLSISAPALAHHDGHGDDDHGHAAHQHNKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(22705..23424)
                     /locus_tag="WJL87_000218"
     CDS             complement(22705..23424)
                     /locus_tag="WJL87_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ7798711.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGKSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAASTSSPNLAIEKRRYEYVVKMGPFASQ
                     ERAAEAEAQARGMVQTVLTAG"
     gene            complement(23421..23543)
                     /locus_tag="WJL87_000219"
     CDS             complement(23421..23543)
                     /locus_tag="WJL87_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ7798712.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            complement(23554..24018)
                     /gene="trmL"
                     /locus_tag="WJL87_000220"
     CDS             complement(23554..24018)
                     /gene="trmL"
                     /locus_tag="WJL87_000220"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ7798713.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVYENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            complement(24083..24580)
                     /locus_tag="WJL87_000221"
     CDS             complement(24083..24580)
                     /locus_tag="WJL87_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ7798714.1"
                     /translation="MWASLHYEPPVSNMIHALKHLADLSMVQPLADLMMQNPPDRLAD
                     ECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQILPRHTVFRHHRPPQSTLKG
                     SERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELAKTLKKSGANRICCWTLAR
                     TPMKK"
     gene            24636..25550
                     /gene="bioH"
                     /locus_tag="WJL87_000222"
     CDS             24636..25550
                     /gene="bioH"
                     /locus_tag="WJL87_000222"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ7798715.1"
                     /translation="MCLKHRGQLFAASVRYAAKSSRQPAQMPSETPDEPWHNTQRLIV
                     GASAMRRQRERKSMPDAVKKVYLIHGWGANRHMFDDLMPRLPATWPVSAVDLPGHGDA
                     PFAQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARL
                     TAAEDYPEGLAAPALGKMVGAFRSDYAKHIKQFLQLQLLHTPDADGIIGRILPDLARC
                     GTPSALQEALDAAERADARHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRLV
                     VMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            25552..26340
                     /locus_tag="WJL87_000223"
     CDS             25552..26340
                     /locus_tag="WJL87_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ7798716.1"
                     /translation="MNPQDKCWQVHRHLAEYADQRLTLVRNAPKYILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAARKGGFWQRFTGKGVVQHCQSPIAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWACALKTDGLLFFTCFGRDTFTELKCRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMAWNDENAA
                     RSCVGTIFEREGGLGITLETVYGHAVKKLMLPQGENVVQFFPKR"
BASE COUNT         5679 a         6749 c         7455 g         6548 t
ORIGIN      
        1 cgaattacgg tgtaaaaatt tatatgacct tataaaatca aataagaatc gttatcataa
       61 catgattgta tttattgggt ttttttgggc gttttgccga tatttacctt ttaatggttt
      121 ttgaaattcg ctaaaatacg aaattattgt agaaattttg ttaacggatt tgggtgtaac
      181 catgttgtcc gcttactttc ccgtctttgt ctttatcctc atcggcctcg cggccggcgt
      241 actgtttatc ctgctcggca caattttggg ctcgaaacgt cactatgccg aaaaagacgc
      301 accttacgaa tgcggttttg aagcctttga aaacgcaagg atgaagttcg acgtgcgcta
      361 ttacctcgtc gccatcctct ttatcttgtt tgatttggag gtcgcgttta tgttgccgtg
      421 ggcagtcgtg ttcaaagatt tgggcgcgta cggcttctgg tctatgctgg tgtttatcgt
      481 cgttctgacg gtaggcttta tttacgaatg gaaaaaaggt gcgctggaat gggaatagaa
      541 ggcgttttga aaaaaggttt catcaccacc agcgcggata cggtgctgaa ctatatgcgt
      601 accggttcgt tgtggccggt tactttcggc ttggcctgct gcgccgtgga aatgatgcac
      661 gcgggtatgg cgcgttatga ccttgaccgt ttcggcatca ttttccgtcc gtccccacgt
      721 caggccgacc tgatgattgt ggcaggtacg ctgaccaaca aaatggcgcc cgccctgcgc
      781 cgcgtgtacg accagcttgc cgagccgcgc tgggtgttgt ctatgggttc gtgtgcaaac
      841 ggcggcggct attatcacta ttcttattcc gtcgtgcgcg gtgccgatcg cgtcgtgccg
      901 gtagacgttt atgtgccggg ttgtccgccg actgcggaag ccctgattta cggcctgatt
      961 cagctccaac aaaaaatcaa gcgcacttcc accatcgcgc gtgacgagta aggagaggac
     1021 gatatggcaa gcattcaaaa cttatacgag accgtcgtcg gcgtgcttgg cgatcaggca
     1081 agcaaagtca tttcagcttt gggcgagatt accgtcgagt gtctgcctga acactatgtt
     1141 tcagtgatga ctacgctgcg cgaccatgaa gaactgcatt tcgagcttct ggtcgatttg
     1201 tgcggtgtcg attacagcac ttacaaaaac gaagtatggc agggcaaacg ctttgccgtc
     1261 gtcagtcagc tgctttctgt tcaaaacaat caacgcatcc gcgtacgcgt ctgggtttca
     1321 gacgacgact tccccgtagt cgaatccgta gtcgatattt acaacagcgc ggattggtac
     1381 gaacgcgaag ccttcgatat gtacggcatt atgttcaaca accacccgga cttgcgccgc
     1441 atcctgaccg attacggctt tgtcggacat ccgttccgca aagacttccc gatttccggc
     1501 tatgtggaaa tgcgttacga cgaagagcaa aaacgcgtga tttaccaacc tgttaccatt
     1561 gagccgcgcg aaatcacgcc gcgtatcgtc cgtgaggaga actacggtgg ccaataaatt
     1621 aagaaactac accatcaact tcggcccgca acaccctgcg gcgcacggcg tattgcgtat
     1681 gattttggag ttggagggcg aaaccatcgt ccgtgccgac ccgcatatcg gccttctgca
     1741 ccgaggcacc gaaaaactgg ctgaaaccaa aacctatctg caagccctgc cctatatgga
     1801 ccgtttggac tacgtttcca tgatggtcaa cgagcaggcg tattgcttgg cggtagaaaa
     1861 acttgccgga attgatgtac ctatccgcgc ccaatatatc cgcgtgatgt tcgccgaagt
     1921 aacgcgcatc ctcaatcact tgatgggcat cggttcgcac gccttcgaca tcggcgcgat
     1981 gaccgccatc ctctacgcct tccgcgatcg cgaagagctg atggacttgt acgaagccgt
     2041 gtccggcgcg cgtatgcacg cagcctattt ccgtcccggc ggcgtttacc gcgacctgcc
     2101 cgacttcatg cccaaatacg agggcagcaa attccgcaat gccaaagtat tgaagcagct
     2161 caacgaatcc cgcgaaggca ccatgctcga ctttatcgat gccttctgcg aacgcttccc
     2221 caaaaatatc gacacactcg aaaccctcct gaccgacaac cgtatttgga aacagcgtac
     2281 cgtcggcatc ggcgtcgtct cccccgaacg tgccatgcaa aaaggcttta ccggcgtgat
     2341 gttgcgcggt tcgggcgtgg aatgggacgt gcgtaagaca cagccttacg aagtgtacga
     2401 caaaatggat ttcgacatcc ctgtcggcgt gaacggcgac tgctacgacc gctacctctg
     2461 ccgtatggaa gaaatgcgtc aatccgtacg catcatcaaa caatgttccg agtggttgcg
     2521 tgtcaatccg ggtccggtca ttaccacaaa ccacaaattc gctccgccca aacgtaccga
     2581 aatgaaaaca ggtatggaag acctgattca ccatttcaaa ctctttaccg agggtatgca
     2641 cgttcccgag ggcgagacct acaccgctgt cgaacatccg aaaggcgagt tcggcgttta
     2701 catcatttca gacggcgcaa acaaacccta ccgcctgaaa atccgcgcac ccggcttcgc
     2761 ccatctgcaa ggcatggacg aaatggcaaa aggccacatg ctcgccgacg tcgttgccat
     2821 catcggtacg caggacatcg tattcgggga ggttgaccga taatgttatc cgcagagtcc
     2881 ttaaaacaaa tcgacacaga actggcgaaa tatcccgccg accaacgccg ttccgccatc
     2941 atgggtgcgt tgcgtatcgc ccaaaccgaa aaaggctggc tcgcccccga aaccatcgct
     3001 tttgttgccg actatatcgg catcacgcct gcacaagcct acgaagtcgc cactttctac
     3061 aatatgtacg accttgagcc tgtcggcaaa tacaaactga ccgtttgtac caacctgccc
     3121 tgcgccctgc gcggcggtat ggctaccggc gaatacctca aacaaaaact cggtatcggc
     3181 tacggcgaaa ctacccctga cggcaagttt acccttgtcg aaggcgaatg catgggcgca
     3241 tgcggcgacg ctcccgttat gctggtcaac aaccacagca tgtgcagctt tatgaccgaa
     3301 gaagcgattg agaagaaact ggcggagttg gagtaggtcg tctgaaacga cgatttaaac
     3361 gtaggtcgga tacttgtagc cgacagagtg ggtaaaaagg caaaatgtcg gatttaagaa
     3421 tccgccctac tgaaataccg aaatgccgtc attcccgcgc aggcgggaat ccaccggtaa
     3481 gattcggttt ctgaatttaa taagacattg cttaccattg aggatggatt cccgcctgcg
     3541 cgggaatgac gacagacaag caagtggtcg agatccaaca aaaacgatta aaggtcgtct
     3601 gaaaatatcg atttgataaa ctagatttta tttcagacga cgttacaagc cggtacacaa
     3661 agacatctta aggtcgtctg aaacagcggc cgcaaccgat acgaaaacaa acaggcacac
     3721 caaaaatggc tatttaccaa tcaggcgtga tttttgacca agtggatacc gccaatcccg
     3781 attgctggac attggacgaa tacgtcaaac gtggcggtta taccgccctg cgtaaaatcc
     3841 tgtccgaaaa catctcgcaa accgatgtga ttgacgaagt caaaacctcc ggcttgcgcg
     3901 gtcgtggcgg tgcgggcttc ccgaccggtt tgaaatggag ctttatgccc cgttctttcc
     3961 ccggcgaaaa gtacgtcgtt tgcaacaccg acgaaggcga gccgggtaca tttaaagacc
     4021 gcgacattat tatgttcaac ccccatgccc tgattgaagg catgattatc gccggttacg
     4081 cgatgggcgc gaaagccggt tacaactata tccacggcga aatttttgaa ggctaccaac
     4141 gctttgaggc cgctttggag caggcgcgtg ccgcaggctt tttgggtaaa aatattttgg
     4201 gttcggattt tgaattcgaa ctcttcgccc accacggcta cggtgcatac atctgcggcg
     4261 aagaaaccgc attgcttgaa tcgctggaag gcaaaaaagg ccagccgcgc tttaaaccgc
     4321 cattccctgc ttcgttcggc ctgtacggca aaccgactac catcaacaat accgaaactt
     4381 tctcctccgt ttcattcatt atccgtgatg gcggacaggc atttgccgat aaaggtattc
     4441 cgaatgcagg cggtaccaaa ttgttctgta tttccggcca tgtcgagcgt ccgggcaact
     4501 atgaagtgcc attgggtacg ccgtttgccg aaatcctgaa aatggcgggc ggtatgcgcg
     4561 gcggcaaaaa actcaaagcc gttattcccg gcggttcgtc tgcgcctgta ttgcctgccg
     4621 atatcatcat gcagaccaat atggactacg actcgatttc caaagccggc tcgatgctcg
     4681 gttccggcgc gattatcgtg atggacgaag acgtgtgcat ggtcaaagcc cttgagcgtc
     4741 tgagctactt ctactacgac gagtcttgcg gtcaatgtac gccttgtcgt gaaggtacgg
     4801 gctggctcta ccgcatcgtc caccgcatcg tagaaggcaa aggccgcatg gaagatttgg
     4861 atttgctgga ttctatcggt aaccaaatgg caggccgcac catctgcgcc ctcgccgatg
     4921 ccgccgtctt ccccgtacgc agctttacca agcatttccg tgatgagttt gtgcattaca
     4981 tcgaacacgg cgggccgatg aaagagcata agtggggagg gtggtaatgg tggaagctaa
     5041 aatttttatt ctatacggtg cagccaacaa aggtaagagt acgacactta atacgctttt
     5101 taatcagatt tgtcggaaat tttctaaatt tctagtcttt tttgaaagac atggaaacgg
     5161 cttagatttt gttgcagtat ttgatcatga aggtcagaga attggttttt attcatctgg
     5221 tgataatgaa tacgaggtta ggagaaattt atacaaactt tattcgcata attgtgattt
     5281 tatttttggc acgtcaagga cacggggtgg tagttgcgat gcagtaggat gttatgcaga
     5341 gttattgcat ggcgatgtaa atataattaa ttggtgtgaa aagtttgagc ctacagatga
     5401 agacaatgag cgtgctgtta aagagttatt taagtcattt aaaaatataa taaataagtt
     5461 atagttttag ttggttttat attggttaaa agcaaaatgc taaaaattta actttgccgt
     5521 cattcccgcg taggcgggaa tccatagtgg aatctaaaga aaccgatatt tgaaaagcag
     5581 ttgccgaaat tcaaaaaatg gattcccgcc tacgcgggaa tgacggcaag gtggcagtaa
     5641 acgttttaaa cagtattgat tgtcaatgaa actcaaaagg ccgtctgaaa cccatttttc
     5701 agacgacctc cataagatta tttatcaaat acccgtaact aggaacgaac catgttacaa
     5761 atcgaaatcg acggcaaaca agtatctgtg gagcagggcg cgacggtgat tgaagccgcg
     5821 cacaagctcg gtacttatat tccgcatttc tgttaccaca aaaaactttc catcgccgcc
     5881 aactgccgta tgtgtctggt gaacgtagaa aaagccccaa aacccctgcc tgcctgtgcc
     5941 acgccggtta cagacggcat gattgtgcgt acgcattcgg caaaagcccg agaggcgcag
     6001 gaaggcgtga tggagttcct gctcatcaac catccgcttg attgtccgac ctgcgaccaa
     6061 ggcggggaat gccagttgca ggatttggcg gtgggctacg gaaaaaccac cagccgctac
     6121 accgaagaaa aacgttccgt cgtcggcaaa gacatggggc ctttggtttc cgccgaggaa
     6181 atgagccgct gtatccactg cacccgctgc gttcgtttca ctgaagaaat cgccggtttg
     6241 caggaaattg cgatggtgaa tcgcggcgaa cactccgaaa tcatgccctt tatcggcaaa
     6301 acggtggaaa ccgagctgtc gggcaacgtc atcgatttgt gtcctgtcgg cgcattgacc
     6361 agcaaaccgt tccgcttcaa cgcgcgtact tgggaattga accgccgcaa atccgtttcc
     6421 gcccatgacg ctttgggcag caacctgatt atacaaacca aagaccatac cgtccgccgc
     6481 gtgttgccgc tggaaaacga agcgattaac gaatgttggc tgtctgaccg cgaccgtttt
     6541 gcctatgaag gcctgtatca cgaaagccgt ttgaaaaacc cgaaaatcaa acagggcggc
     6601 gagtggatgg acgtggattg gaaaaccgcg ttggaatatg tccgcagcgc gattgaatgt
     6661 atcgccaaag acggcaacca aaatcaagtc ggcgtttggg ccaatccgat gaatacggtt
     6721 gaagagctgt atttggccaa aaagcttgcc gacggcttgg gtgttaaaaa ctttgccacc
     6781 cgtctgcgcc aacaagacaa acgtctttca gacggcatta aaggtgcgca atggttggga
     6841 caaagcattg aatcgctggc ggacaacgat gccgtattgg tagtcggtgc gaacttgcgc
     6901 aaagaacagc cgctcctgac cgcccgcctg cgccgcgccg ccaaagaccg tatggccttg
     6961 agcgtattgg ccggcagcaa ggaagagttg ttgatgccgc ttctctctca agaagccgca
     7021 catcccgacg agtgggcagg ccgtctgaaa aacctgtctg ccaatgcgga acacgccgtt
     7081 accgccagcc tgaaaaatgc tgaaaaagca gcggtgattt tgggcgcgga agtgcaaaac
     7141 catcctgatt acgccgccat ttatgctgcc gcgcaagagc tggcagccgc gaccggcgca
     7201 gtgctgggta ttttgccgca agccgccaac agcgttggtg cggatgtctt gggcgtgaac
     7261 tcgggcgaga gcgttgccga aatggcaaac gcgccgaaac aggcggtctt gctactcaac
     7321 gtcgaaccgg aaatcgatac ggcggacggt gcgaaagccg tggccgcgtt gaaacaggcg
     7381 aaaagcgtga tggcgtttac accgtttgtc agcgaaacgc tgctggatgt gtgcgacgtg
     7441 ttgttgccga ttgcgccgtt taccgaaacc tcgggcagct tcatcaatat ggaaggccgt
     7501 ctgcaatcct tccacggcgt ggtacaaggc ttcggcgatt cgcgtccgat gtggaaagta
     7561 ttgcgcgtat tgggcaacct gtttgacctg caaggttttg aataccacga taccgctgcg
     7621 attctgaaag atgcgctgga tgtagaaagc ctgccgtcca aactggacaa ccgcaacaca
     7681 tggacagggg agggcgttca gacgacctca gaccgcctcg tccgtgtcgg cggcgtcggt
     7741 atttatcaca ctgattctat cgtgcgccgt tccgcaccgt tgcaagaaac cggccatgcc
     7801 gccgtgcccg ccgcgcgtgt caatccgaac actttggcac gcttgggcct gcaaaacgga
     7861 caaactgctg tcgccaaaca aaacggcgca agcgtatcgg ttgccgtcaa agccgatgcc
     7921 ggtttgcctg aaaacgtggt gcatctgccg ctgcataccg aaaatgccgc gctgggtgcg
     7981 ttgatggaca ctattgaact ggcgggagct tgattatgca ggaatggttc caaaacctct
     8041 ttgccgcaac gctcggtctg ggcgatttgg gtattaccgt aggtttggtg gtatccgtca
     8101 tcgtcaaaat cgtgattatc ctgattccgc tgattctgac cgttgcctac ctgacgtatt
     8161 tcgaacgtaa agtcatcggc tttatgcagc ttcgtgtcgg cccgaacgta accggcccgt
     8221 ggggtctgat tcagccgttt gccgacgtgt tcaaactctt gtttaaagag gtaacccgtc
     8281 cgaagctgtc aaacaaagcc ctgttctaca tcggcccgat tatgtcgctc gccccgtctt
     8341 tcgcggcgtg ggcagtgatt ccgttcaacg aagaatgggt gctgaccaac atcaatatcg
     8401 gccttttgta catcctgatg attacctcgc tgtcggttta cggcgtgatc atcgcgggct
     8461 gggcttccaa ctccaaatat tcgttcttgg gtgcaatgcg tgcttccgca caaagcattt
     8521 cctacgagat tgccatgagt gccgcgctgg tgtgtgtcgt gatggtgtcg ggcagcatga
     8581 acttctccga catcgttgcc gcgcaggcaa aaggtatcgc gggcggttcc gtattctcgt
     8641 ggaactggct gccgctgttc ccaatcttca tcgtctatct gatttccgcc gttgccgaaa
     8701 ccaaccgcgc accgtttgac gtggcagagg gcgagtccga aatcgttgcc ggacaccacg
     8761 ttgaatattc cggcttcgca ttcgcgctgt tcttccttgc cgaatacatt ttcatgattc
     8821 tgattgccgc gctgacatcg ctgatgtttc tcggcggctg gttgtctccg ttcccgcaaa
     8881 gctggggctt tatcggtacg ccttccgcat tctggatgtt tgtgaaaatg gcggcagtgt
     8941 tgtactggta cctgtggatc cgtgcaacct tcccgcgcta ccgttacgac caaatcatgc
     9001 gtttgggctg gaaagtgctg attccgatcg gcttcgccta catcgtgatt ttgggcgtgt
     9061 ggatgatttc accgctgaat ttgtggaaat aagtttcaga cggcatcttg aggccgtctg
     9121 aacaaagcga ttttgaatac ctaacgaaat ccctgttttg agggaacata atatggctaa
     9181 cttagtaaaa acctttctgc tcggcgagtt ggtaaaaggc atgggcgtaa cgctcaaaaa
     9241 cttttttgcc cgcaaagaca caatttattt ccccgaagag aaaacgccgc aatccgtgcg
     9301 tttccgtggc ctgcacgcgc agcggcggta tccgaacggc gaagagcgct gtatcgcgtg
     9361 taaattgtgc gaggcagttt gtccggcaat ggcgattaac attgaatcgg aagaacgcga
     9421 agacggcacc cgccgcacca agcgttacga tatcgacctg accaagtgca tcttctgcgg
     9481 tttctgcgaa gaggcctgcc cgaccgatgc gattgtggaa acgcatattt ttgaatacca
     9541 cggcgagaaa aaaggcgact tgcacatgac caagccgatc ctcttggcaa tcggcgacaa
     9601 atacgaagcc gaaatcgcca aacgcaaagc cgctgacgcg ccgtatcgtt aaggagcaga
     9661 cgaatgactt tttccgcgat tctgttctat atccttgccg ccatcgtcct atacggcgcg
     9721 ctgcgtaccg ttacagcgaa aaaccctgtc cacgccgctt tgcatctggt gttgaccttc
     9781 tgcgtgagcg cgatgctttg gatgctgatg caggctgagt ttttgggcgt gacgctggtg
     9841 gtggtttacg tcggcgccgt gatggtattg ttcctgttcg tcgtgatgat gttgaacatc
     9901 gacattgaag aaatgcgtgc cggtttctgg cggcacgcgc ctgttgccgg tgtggtcggc
     9961 acattgttgg cagttgccct gattctgatt ctggtcaatc cgaaaaccga ccttgccgca
    10021 tttgggctga tgaaagacat tcccgccgat tacaacaata tccgcgattt gggcagccgt
    10081 atttataccg attatctgct gccgtttgaa ttggcggcgg tattgctgct gttgggtatg
    10141 gtggccgcga ttgcgctggt tcaccgtaaa acggttaatc cgaaacgtat ggatcctgcc
    10201 gaccaagtta aagtacgcgc cgaccaaggc cgtatgcgct tggtgaaaat ggaagccgtg
    10261 aaaccgcaaa ccgaatctgc cgaagaaagc gaagtttcag acgacctcaa gccgaaagag
    10321 gagggcaaag catgattacc ttgacgcatt atctggtatt gggtgcgctc ctgttcggta
    10381 tcagcgcgat gggtatcttc atgaaccgca aaaacgtgct ggtattgttg atgtccattg
    10441 agctgatgct tttggcggtg aacttcaact ttatcgcctt ttcgcaacat ttgggcgata
    10501 ctgccggaca aattttcgta tttttcgtac tgaccgttgc tgctgccgaa tctgccatcg
    10561 gtttggcaat tatggtgctg gtgtaccgca accgacaaac gattaacgtt gccgatttgg
    10621 acgagttgaa agggtaaagg taggttgggt cgagacctga caagacaccg atgccgtctg
    10681 aaaacccgat aggaaaaacg atgaaatcca tagacgaaca aagcctgcat aatgcccgcc
    10741 gcctgtttga aagcggcgac atcgaccgta tcgaagtcgg taccaccgcg ggcctgcaac
    10801 agattcaccg ttacctgttc ggcggcttat atgattttgc gggtcaaatc agggaagaca
    10861 acatttccaa aggcggtttt cgttttgcca acgccatgta tttaaaagag gctttggtta
    10921 aaatcgagca gatgcccgag cggacttttg aagaaatcat cgccaaatat gttgaaatga
    10981 acattgccca tccgtttttg gagggtaatg gcagaagtac ccgcatctgg ctggatttgg
    11041 tgctgaaaaa aaacctgaaa aaagtcgtga actggcaaaa tgtaagtaaa accctgtatt
    11101 tgcaggcaat ggaacgcagc cccgtcaacg atttagaact gcgctttctg ttaaaggaca
    11161 acctgactga cgatgtggac aaccgtgaaa tcatctttaa aggtatcgag cagtcgtatt
    11221 attacgaagg gtatgaaaaa ggctgagggt cgtctgaaaa gcgatttcag actgtttcag
    11281 acgacctgat tcggtaggtg atcagacggg agcggatgag aaaagaaatt ctgggtaaga
    11341 ataatccggt ctgaaatatt ggaagaagaa tgatggataa aaatcagtta gaacaagaat
    11401 ttcataaagc catgttaaat atttatcagg aggctttgaa tttgccgcaa ccttacaagg
    11461 cgacacgatt tttacaaatt gtaaatgaat ttggtggtaa agaggcggcg gataaattat
    11521 taagtacggg ggaaaagaag actcagaccg gttttacaga gctgattttg agtggtggcg
    11581 gagtccacgc cttgaaatac agtatggaat atctggtgtt acaaaagccg tggtgtgatt
    11641 tatttactga agagcaatta gctgtggcac gcaaacgatt ggagcgtgtt ggatttgttt
    11701 ctccgaagta attttgtacg aaacaaacat agatttttaa atcaatcgga ttcaatcaaa
    11761 tgaacgacat gactttatat ctcatcattg cccttgtgcc tttggcaggc tcgctgatcg
    11821 cgggtttgtt cggcaacaaa atcggacgtg ccggtgcgca tacggttacg atactcggcg
    11881 tggcggtgtc cgccgtgctg tcggcttatg tgttgtgggg ctttattgac ggcagccgcg
    11941 ccaagtttga cgaaaacgtc tatacctggc tgacaatggg cggcttggat ttctccgtcg
    12001 gctttttggt cgatacgatg acggcgatga tgatggtcgt ggtcaccggc gtatcgctga
    12061 tggtacatat ctataccatc ggttatatgc acgatgaaaa aatcggctac caacgcttct
    12121 tcagctatat ttctttgttt actttcagca tgttgatgct gattatgagc aacaacttca
    12181 tccagctctt cttcggttgg gaagcggtgg gcttggtgtc gtatctcttg atcggtttct
    12241 atttcaaacg tccgagcgcg acatttgcca acctgaaagc ctttttgatc aaccgtgtcg
    12301 gcgacttcgg ctttttgctc ggtatcggct tggtgcttgc ctatttcggc ggcagcctgc
    12361 gctatcaaga tgtattcgct tatctgccta atgttcagac ggccaccatt caattgttcc
    12421 ccagtgtgga atggtctttg attactgtaa cctgtttgct cctgtttgtc ggtgcgatgg
    12481 gtaaatcggc acaattcccg ctgcacgtct ggctgcctga ttcgatggaa ggcccgaccc
    12541 cgatttctgc attgattcac gccgcaacca tggttaccgc cggtctgttc atggtatcgc
    12601 gtatgtcgcc gatttatgag atgagcagca ctgcgctgtc tgtcatcatg gtaatcggtg
    12661 cgattaccgc cctgtttatg ggtttcttgg gcgtgattca aaacgacatc aagcgcgtgg
    12721 ttgcgtattc caccctgtcg caactgggct atatgactgt ggctctgggc gcgtctgcct
    12781 attccgtggc gatgttccat gtgatgaccc acgctttctt taaagccttg ttgttcttgg
    12841 ccgcaggtag cgcgattatc ggtatgcatc acgaccaaga catgcgccac atgggcaacc
    12901 tgaaaaaata catgccgatt acttggttga ccatgttgat cggtaacttg tcgctgattg
    12961 gtacgccgtt cttctccggc ttttactcca aagattcgat tatcgaagcg gcgaaataca
    13021 gcacccttcc cggcagcggc tttgcctatt ttgccgtcct tgccagcgtg ttcgttaccg
    13081 cgttttacgc gttccgccaa tactttatgg tgttccacgg cgaagagaaa tggcgcagcc
    13141 tgcccgaaca ccattcagac ggacatggcg aagaacatca cggcttgggc aaaaacgaca
    13201 atccgcacga aagcccgctg ttcgttaccc tgcctttgat tttgcttgcc attccgtccg
    13261 tcatcatcgg ctatgttgcc atcgggccta tgctttacgg cgatttcttc aaagacgtga
    13321 ttttcgtcaa taccgacgcg catccgacca tgcacatcat gaaggaagag ttccacggcg
    13381 cattggcaat ggtgtcgcac agcctgcatt ctcctgtact ctacctcgct atcgcaggtg
    13441 tattgagcgc atggcttttg tacgtcaaac tgccgcacct gcctgcgaaa attgcacagg
    13501 cgttccgtcc ggtttacgtt ttgtttgaaa acaaatacta cctcgacgcc ctgtatttca
    13561 acgtcttcgc taaaggcacg cgcgcactgg gtaccttctt ctggaaagtc ggcgataccg
    13621 ccattatcga taacggcatc gtcaacggct ctgccaaact ggtcggcgcg attgccgcgc
    13681 aagtgcgtaa agcccaaacc ggctttatct acacctacgc cgccgctatg gtgttcggcg
    13741 tattggtact gctcggcatg accttctggg gattgttcag ataaggatga ggcttcagac
    13801 ggtctcttaa aacgtcgtct gacaaaccaa cccgtacaac gatattttta ttttaaccac
    13861 aggttaacca ctatgttttc caactaccta ctcagcttgg caatatggat acccatcgcc
    13921 gcaggtgtgc tggttttggc aacggggtcg gacagccgtg cgccgctggc gcgtgtgctt
    13981 gccttcatgg gtgcgcttgc cggtttcttg gtaacgctgc ccctgtttac cggtttcgac
    14041 cgtttgagcg gcggctatca atttaccgag ttccacgagt ggattccgct gctgaaaatc
    14101 aactatgcat tgggcgtgga cggtatttca gtgctcttta tcatcttgaa tgcgtttatt
    14161 acgctgttgg tggtattggc cggttgggaa gttattcaga aacgtccggc gcagtatatg
    14221 gcggcattcc tgatgatgtc gggtttgatt aacggcgcgt ttgccgcaca ggatgcgatt
    14281 ctgttttatg tgttcttcga gggtatgctg attccgctgt acctgattat cggtgtatgg
    14341 ggtggcccgc gccgcgttta tgcgtcggtc aagctgttcc tctatacgct gatgggttcg
    14401 cttttgatgc tggttgccat ggtttacctg tactatcaga caggcagctt ctctattgtc
    14461 gatttccaaa acatcaagca gattccattg ggcgtacaac agcttttgtt tgtggcattc
    14521 ttcctgtcat ttgccgtaaa agtgccgatg ttccctgtgc acacttggtt gccggatgcc
    14581 cacgttgaag cgccgaccgg cggttcgatg gtgttggcgg caattacgct gaaactgggt
    14641 gcgtatggtt tcttgcgttt tatcctgccg attatgccgg atgcggcacg ctattttgcc
    14701 cctgtgatca tcgtattgag cctgattgcc gtgatttata tcggtatggt ggctttggtg
    14761 caaaccgata tgaaaaaact ggtggcgtat tcgtctatca gccatatggg ttttgtaacg
    14821 cttgggatgt ttttgtttgt tgacgggcag ttggacgact gggcgttgaa aggcgcaatc
    14881 attcaaatga tttcgcacgg tttcgtgtcg gcggcgatgt ttatgtgtat cggcgtgatg
    14941 tacgaccgcc tgcatacccg caatattgct gactatggcg gcgtggtcaa tgtgatgcct
    15001 aagtttgcgg cgtttatgat gttgttcggt atggccaatg ccggtctgcc tgcgacttcc
    15061 ggcttcgtgg gcgagtttat ggtgattatg ggcgcggtta aagtgaattt ctgggtaggc
    15121 gcgttggcgg ctatgacgct gatttacggc gcgtcttata ccctgtggat gtacaagcgc
    15181 gtgatttacg gcgcgatcca caatccgcac gttgccgaaa tgaaagacat caactgtcgc
    15241 gagtttgcga ttctggcggt tttggcggtt gcggtattgg gtatgggctt gtatccgaac
    15301 gcgtttatcg aagtggtgca tcaggcggca aacgatttga ttgcccatgt ggcacaaagc
    15361 aagatttgag gtgtgtaaat gaactggtct gatttgaatt taatgcccgc actgcccgaa
    15421 atcgcgctgc tgtcgctgct ggtgttattg ttgctggcag acttgtgggt cagtgatgac
    15481 aaacgccgtt ggacgcatta cggcgcgttg gcaacggtgg cggttacggc tgtggtgcag
    15541 cttgccgttt gggaacaggg cagcacgtct tcgttcaacg gcatgtatat tgcagacggt
    15601 atgtcgcgtt tggcaaaaat ggttttatat gccttgacgt ttgccctgtt tgtttatgcc
    15661 aagccctaca accaagtgcg cggtattttc aaaggcgagt tttacaccct gtcgctgttt
    15721 gccctcttgg gcatgagcgt gatggtgagc acggggcatt ttttaactgc ctatatcggt
    15781 ttggaactct tgtcgcttgc cctttacgcc ctgattgccc tgcgccgcga ttccggcttt
    15841 gccgccgaag ccgccttgaa atattttgtt ttgggcgcgc tggcatccgg cctgctgctc
    15901 tacggtattt ctatggttta cggcgcaacc ggttcgctgg aatttgccgg cgtgctcgcc
    15961 tcttccttca atgaagaagc caacgaatgg ctgttgaaac tgggtttggt gtttatcgtc
    16021 gtcgccgtcg cgttcaaact cggtgcggtg ccgttccata tgtgggtgcc cgacgtgtat
    16081 cacggcgcgc ccacttctgt taccgccctg gtcggcactg ccccgaaagt cgccgccgtc
    16141 gttttcgctt tccgcatcct cgttaccggg ctgggaaccg tgcatcatga ctggtctctg
    16201 atgtttgccc tgcttgccgc cgcctcgctg ctggtcggca accttgccgc catcatgcag
    16261 accaatatca aacgtatgct cgcctattcc accgtatcgc atatggggtt tatcctgctg
    16321 gcgtttatgg cgggcgcggt cggctttgcg gcgggtctct attacgccat tacctacgcg
    16381 ctgatggcgg cggcagggtt cggcgtgttg atggtgttgt cggatgggga caacgagtgc
    16441 gaaaacatca gcgatttggc agggctgaac caacaccgcg tatggcttgc ctttttgatg
    16501 ttactggtta tgttctctat ggcgggcatt ccgccgctga tgggttttta cgccaaattt
    16561 ggcgtgatta tggcgctctt gaaacaaggc catgtttggt tgtccgtatt tgccgtcgtg
    16621 atgtcgctga ttggtgcgtt ctactacctg cgcgtggtca aagtcatgta ttttgacgaa
    16681 tccggccgcg cccgtcccac cgtcggcggc aacaatgccg caaaattcct tttgagcgtc
    16741 aacgcgctgc tgttggtgct gtggggcatt atgccgcaaa ccgttatcga ctggtgcgcc
    16801 aaggcgttgg aaaacacgct gtaagccgcc gcaacggcag ccgtgtcaga ggctgccgtt
    16861 tttgttaaga tatgccgtcc cgcaacgcag ttcagacggc atcgccgccg acaatgccca
    16921 aacagaaagc ccgccatgac cgcatccatg tacatccttt tgctgcttgc cttgattttt
    16981 gccaacgccc ccttcctcac gaccaggctg ttcggcatcg taccgctcaa gcgcaaacat
    17041 ttcgggcacc acttgatcga gctggcggca ggtttcgcgc tgactgccgt tcttgcctac
    17101 atcctcgaat cccgtgcggg agcggtacac gatcagggtt gggagtttta cgccaccgtc
    17161 gtctgcctgt acctgatttt tgcgtttccc tgtttcgtgt ggcggtattt ttggcacacg
    17221 cgcaacaggg aataggcagg cataggaatg ccgtctgaaa ccctttcaga cggcatttgt
    17281 ttcattcaag tgcaggccgg catcgctgtg ccggcacgtt tcagccggcg atatacgccg
    17341 gttttaatat ttgcgggcga ctgcaaattc tgccaactgc cgcaggcgca gggctttgtc
    17401 gccgaagggt tcgagcagcg cgaccgcttc ggcaaccagt ttgtgtgcgt atgagcgcgc
    17461 cgcttccaag cccatcagtt tcacataagt cggcttgtcg ttgtctgcgt ctttgcccgc
    17521 cgttttgcct aaagtcgccg tgtccgcttc acaatccaac acatcgtcaa tgacttggaa
    17581 cgccagcccc agttttgccg cataatcgtc caataccgcc agactgtctt cagacagatt
    17641 cgggcaggac atcgccccca ataaaaccgc cgcacggatt agcgcacccg ttttcaggct
    17701 gtgcatctgt tccaaatcgg cttgaaccat ttgtttgccg acattcgcca aatcgattgc
    17761 ctgaccgccc gccatgcccg cgctgccgcc cgctttcgcc aacaccgaca acattgccaa
    17821 ctggcgtgct gcgggcagtt ctgtcggacg gcttaacacg tcgaaggctt gggtttgcaa
    17881 agcgtcgccg accagtagcg cggtggcttc gtcgtattgt atatggcaag tcggtttgcc
    17941 gcgccgcagg ctgtcgttgt ccatcgccgg catatcgtcg tgaaccaaag aatagacgtg
    18001 gatcatttcg attgccgcca ttgcctgttc tactgcttca tgcacggctt cgcctaattc
    18061 cgaagctgcc agaaccagca tcggccgcag acgcttaccg ccgtccaaag ccgcataacg
    18121 catcgcttcg tgcagcgtgt gcgggatttc gttttcagac ggcaaaaagc gttccagcag
    18181 cagctctgtc tgtgcctgcg ccctctgttg ccacgctttc aaatcattcg tcggattcaa
    18241 ggttcaactc cttcagcccg tccgcatcca aaacctgtaa tttctgttcg acttgtgcca
    18301 gcttggtttg gcagtacctg accagctcgt tgccttcctg ataggcggca agcgcgtctt
    18361 ccaagggcat ttcgccctgc atagactgcg tcagcgattc aaggcgcgac aaggcttctt
    18421 caaacgattt cggggcgttt ttcttcatcg tgtttccttt tcggttgaaa ccccgccact
    18481 tggacatctg tccttcgggg cggtagaatc agactttatt tgggaggggt gtaagccctt
    18541 ccaaatcagg acgacacata gggcggtgct ttaagtgtcg tcctgcgtgt tggaacatag
    18601 tttcggatgt tccggtaaaa agcggattgt agcatttttg aaaaacggat gccgtctgaa
    18661 acccgaatcc ggcttcagac ggcattgttt tgtcctgcaa acggcaaggc gttacccggg
    18721 cagttcgtcg gtgatgccct gcaaaaaggc gaggcgttcg gggcttgccg ccccggtttc
    18781 ggcggcggct ttgaaggcgc agccgggttc ggcgcggtgg gtgcagttgt ggaagcggca
    18841 ttgcccgaca aggtggcgga aatcggggaa atagcgcggc aaatcggcgg cttggaggtg
    18901 gtgtaaacca aattcttgca aacccgggga gtcgatgagt tgggtttcgc cgttcaaatc
    18961 ataaagccgg gcgtgggtgg tggtgtgttt tcccgagtcg agtgcggcgg aaatgtcgcc
    19021 ggtgcgggcg gtttggctgc ccaaaagggc gttggtcagg gtggatttgc ccataccgct
    19081 ctgcccgagc aggatgttgc tgtgcccttg cagggcgggg cgcaggctgc cggcgttttc
    19141 cagtgcgcgg gtttcgatga cgggataacc cagcgtttcg tagaatttga gtttttcgcg
    19201 ccaaagggcg gtttcgggca ggtcggcttt gttcaggacg atgacggcgc ggatgccggc
    19261 ggcttcggcg gcaagcaggg cgcgctgtag cagccgcacg ctcggactcg ggacggcggc
    19321 ggttacgatg aggagttggg taacgttggc ggcaatgagt ttggttttcc acgcgtcttg
    19381 gcggtagagc aggctttggc gcggtaaaaa atcttcaatc acaacttgtt cggcgttgac
    19441 ggggctgatg cggacacggt cgccgcaggc gaaatcgacg cgttttttgc gcgtgctggc
    19501 ttcgtaggtt gtgccgtcgg gcgtgcgcac gatgtagcgg cggccgtagc tggcggtaat
    19561 ttgggcggtg tcgttcatgg tttctttggg gttgggtgtg gaaatgccgt ctgaaaacgg
    19621 gtgttcggac ggcatcggtt cagtcgtgct gccactcgac gtgttcgttg aggaagccgc
    19681 cgctctggtg cgcccagagt ttggcgtaaa gcccgcgttt ttcgaggagt tcggcgtgcg
    19741 tgccttcttc gatgatgcgg cctttgtcga ggacgacgag cctgtccatt gcggcgatgg
    19801 tggagaggcg gtgggcgatg gcgatgacgg ttttgccgtc catcattttg tcgaggcttt
    19861 cttggatggc ggcttcgact tcggaatcga gcgcgctggt ggcttcgtcc aaaagaagaa
    19921 tcggtgcgtc tttgagcatc acgcgggcga tggcgatgcg ctggcgttga ccgccggaga
    19981 gtttcacgcc gcgttcgccg acgtgtgcgt cgtagccgcg ccgccctttg gcatcggaaa
    20041 ggtcggggat gaagccggcg gcttcggcgc gttcggcggc agaaaccatt tcggcatcgg
    20101 tcgcgtcggg gcggccgtaa ataatgttgt cgcgcacgga acggtgcagc agcgaggtat
    20161 cttgcgtgac caaaccgatt tgggcgcgta aagattcttg ggtgatgccg cttatgtcct
    20221 gcccgtcgat cgaaaccgtg ccgctttgcg gttcgtagaa gcgcaaaagc aggttgacga
    20281 tggtggattt gcccgcgccg ctgcgtccga tcaagccgac cttttcgccg ggtcggatgg
    20341 tgaggttgaa gccgttgagc agcggtttgc cggcttcgta gcagaaatcg atgtgttcaa
    20401 atttgattgc gccttgcgac actttcaacg gcagggcgtt cggcttgtcg aggatggtgt
    20461 gcggtttgga cagggttgcc atgccgtcgc cgacggtgcc gatgttttca aacagctgcg
    20521 cggattccca cataatgtat tgcgacaaac cgttgacgcg caacgccatg gcggtggctg
    20581 tagcaaccgc gcccacgccg acctgcccgt tgtgccagag ccagatgccc agtgcggcgg
    20641 tggagagggt cagggaggtg ttgacgatga agctgcacga atgcagcagc gtcgccagcc
    20701 gcatttgggc gcgcaccgta accataaatt cttccatcga ctgcttggca taggcggctt
    20761 cacgcgcgcc gtgggagaag agtttgacgg tggcgatatt ggaataggca tcggtaatgc
    20821 ggccggtcat cagcgagcgg gcatccgcct gccatgcggc ggtttgcccc aatttgggaa
    20881 tcagcaggcg catcaccgaa gcgaaaccga caatccaacc gataaagggc agcagcagcc
    20941 atgagtcgag cgaggcgaga atcacgccgg aggtaatgaa atacaccgac acataaacga
    21001 ccatatcggc aaccgtcatc accgcgtcgc gcaacgccag cgcggtctgc atgactttgg
    21061 cggacacgcg tccggcaaat tcgtcctgat aaaaaccgag gctttggttc agcatcaggc
    21121 ggtggaagtt ccagcgcagg cgcataggga acacgccctg aagggtttgc aggcgcacgt
    21181 tggacgcggc aaacgcccac gcaaccgaaa ataccatcat cgccgccatt gccgccagtt
    21241 cccaaccttt ttcggcaaac agttcggcgg gcgcgtattt gccgagccac tccacgattt
    21301 tgcccataaa ttgaaaaacc agggcttcca taatgccgat gccggcggtc agcgcagcca
    21361 gggcggctat ccatttccgc acgccggcca tgctgctcca gacaaaccgc cacaagcctt
    21421 tttctggcgt tttcggggcg gcttcgggat aagggtcgat tcgggactcg aaccaggaaa
    21481 atattttgtt caacattgtt ttcgatttcg gtaaaacagt ttcagacggc atcaaacaca
    21541 atgccgtctg aaaggaagga caataacgcc attttacggg aaaagccgtc gggaagacag
    21601 cgtgaggcgg aaacgcaggg tttcgtcagg gcaaacgccg cgccgccttc agacggcatt
    21661 atttcagcag gtttttcaaa gcaaggcgca cgccttcgcc cacgtccgtc ccctccggaa
    21721 cgcctttgac cgccgctttt gcttcgcgtt cgctgtaacc cagcgcaagc agcgtgctga
    21781 cgatgtcttc cgtttcgtcg gcggcgggtg cggcggcaaa cagcccgtcc gttaccgtat
    21841 gcgcgaccag cttgccgcgc agttccaaga ccatacgttc ggcggttttt ttgccgattc
    21901 ccggcgcgga agacaggcgt ttgacatctt cttcggcaac cgcccgcgcc agctcgtctg
    21961 ccgtcatcgc tgacaaaatg cccaaagccg ttttcgcgcc gatgccgccg accttaatca
    22021 gttggcggaa ggtcttgcgt tcttccgcag tggcaaaacc aaataaaaga tgtgcgtctt
    22081 cccgaatgat aagctgggta aacagttgta cgctttcacc cacgggcggc aggttgtaga
    22141 aggtctgcat cgatacgtcg gcctcatagc cgacaccgtt gacatcgatg acgatttgcg
    22201 gagggttttt ttcaaccagt ttgccggtca gtctgctgat catgtgtgcc gaatcctgaa
    22261 gtgtcgggtg caaaatgccg tctgaaaccg gttcgggctt cagacggcac ggattgtatc
    22321 aaattcagtc gtcgcggcgg gaggaaatca cgcggccggt acgggcatcg acaacgactt
    22381 tgtattcctg tccgtttttg acgatttcga catcatagtg cggacggccg ttgtcgtgtt
    22441 cgagatcgat gtcggtgatt ttgccgccga cacgcgccaa cgctgctttt tcggcttggg
    22501 cgcggctgat gattttgtct tgtttgttgt gttggtgtgc ggcgtgtccg tggtcgtcat
    22561 cgccgtgtcc gtcgtggtgg gcgagcgcgg gggcggaaat gctcagcagt gcggttgcgg
    22621 cggcggtcaa gagaaggcgt ttgaagttca tgttttgctc ctgcgaagta tctggtggtg
    22681 tttgaaagac gtaaaggcgg gacatcaacc ggcggtcaat accgtctgaa ccataccgcg
    22741 cgcctgagct tcggcctcgg cggcgcgttc ctgcgaggca aacggaccca ttttgacgac
    22801 gtattcgtaa cggcgttttt caatggcgag gttcgggctt gatgtcgaag cggcgaagtt
    22861 ttgggcggct tggttcagat aggcttgtgc ttcgtgttcc gtaccgaaag atttcaagtc
    22921 gataaagatg tctttgtttt cggcaaccgg tgcggatttg cccgggacga tttgttcgat
    22981 tttgacgtgc gccgtccctt ggctgacaaa gcccaatttt tgcgcggcgg ctttggatac
    23041 gtcgatgatg cggttgccgt ggaaggggcc gcggtcgttg acgcggacga tgacgctttt
    23101 gccgtttttg gtattggtta cgcgcacata gctggggatg ggcagggttt tgtgggcggc
    23161 ggtaaaggcg ttcatatcgt atcgttctcc gccggaagtt ttgcgcccgt gaaacctgcc
    23221 gccgtaccac gaggcgttgc cggtttgcgt gaattcggcg acttggtttt tcggcgtgta
    23281 gcgttttccg gcgactttgt agctgcggtt ggcggaggcg tgcagttttt ctgccttgac
    23341 cactgcgtcg gcggatgccg tctgaaggga gtgtgtgccg aatgcggcgg tgagaaggaa
    23401 aagggttttt cgggttaaag tcaaaacgtg ttccgttctt gagttgaaga cgaatgggca
    23461 tcatgcccgc cggatacgtt ccgaaccgcc gtacagtgcg gacggcggtt cggaatgtgt
    23521 ccggataggt tttcagacgg catgaacttg cgttcaaacg ccgcctgcgt aaccgtgttg
    23581 ccgccacgct tcaaagagaa tcacggcaac ggtattggaa aggttcatac tccggctgcc
    23641 gggctgcatg ggcaggcgga ttttttgcgc ggcgggcagg ctgtcgagga tgtcggcagg
    23701 cagtccgcgc gtttccggcc cgaacagtaa aacgtcgcct ttttgaaacg cggtttcatc
    23761 ggggcgcgcc gtgcctttgg tggtcagggc gaaaatgcgc ctgcctgcga gtgccttgag
    23821 gcagtcgtcg aagttttcgt acaccgtcag gctggcgaat tcgtggtagt cgagcccggc
    23881 gcgtttcatt ttggcggaat ccaatgggaa gccgagcggt ttgacaaggt gcaaatccgc
    23941 gccggtattg gcgcacaggc ggatgatgtt gcccgtgttc ggcgggattt ccggctggta
    24001 taaaacgatg gtaaacataa atatcaatca cttataggcg cgtaaccttg ccacaaggcg
    24061 gatggggtgt caaaaaattt agttattttt tcattggcgt gcgtgccagc gtccagcagc
    24121 agatgcggtt cgcgcccgat tttttcagcg tctttgccaa ttcgtccagc gtcgcgccgg
    24181 tggtaaagac atcgtcgatt aacagaatat tacagttttc cggtatcggt gtgcggattt
    24241 caaaggcgtt tttgatgttt cgccgccgtt cgctgccttt gagcgtgctt tgcggcgggc
    24301 ggtggtgtcg gaatacggtg tgtcggggca gtatctgcca gccgtagcgt tgtgccaaca
    24361 acccgacgat gctttcactt tggttgaacc cgcgttgcag cagccgctcc ctgcttagcg
    24421 gtacgggcag gacgaaatcg aaacattcgt ctgcaagccg gtcgggcgga ttctgcatca
    24481 tcaggtctgc cagcggctgc accatgctca aatcagccaa gtgcttcagc gcgtgtatca
    24541 tattgctgac gggcggttcg taatgcagcg aagcccacat ccggtcgaat gcgggcggtt
    24601 ttttctgaca gccgccgcac accgcgccgc cttggatgtg cctgaaacac agggggcagc
    24661 tgtttgccgc gtcggtgcgg tatgccgcca aatcgtcgcg gcagccggcg cagatgccgt
    24721 ctgaaacgcc ggacgaaccg tggcataata cgcaacgcct gatagtgggc gcgtctgcga
    24781 tgcgccgcca acgagagaga aaatccatgc ctgatgccgt caaaaaagtt tacctgatac
    24841 acggttgggg ggcgaaccgc cacatgttcg acgatttgat gccgcgcctg cctgcaacgt
    24901 ggccggtgtc cgccgtcgat ttgcccggac acggcgacgc gcccttcgcg caacccttcg
    24961 atattgaggc agccgccgac gctgttgccg cacaaatcga tacgcccgcc gacattctcg
    25021 gctggtcgct cggcggattg gtcgcgctgt atctggcggc gcgccatccc gacaaagtcc
    25081 gttcgctctg cctgacggcg agtttcgcac ggctgacggc tgccgaagat tatcccgaag
    25141 ggcttgccgc gcctgcactg ggcaaaatgg tcggcgcgtt ccgttcggat tatgccaaac
    25201 atatcaaaca gtttctacaa ctccagcttc tgcacacgcc tgatgcggac ggaatcatag
    25261 gcagaatcct gcccgatttg gcacggtgcg gcacgccctc cgccttacag gaagctttgg
    25321 acgcggcgga aagggcggat gcgcggcatt tgttggacaa gatagatgtt ccggtactgc
    25381 tggtgttcgg cggcaaagac gcgattacgc cgccgcgtat gggcgagtat ctgcaccggc
    25441 atttgaaagg cagcaggctg gttgtgatgg aaaaggcggc acacgcgccg tttttgagcc
    25501 atgcggaagc gtttgccgcg ctgtaccgcg actttgttga aggggtttga catgaatccg
    25561 caggacaaat gctggcaggt tcaccgccat ctcgccgaat atgccgacca acgcctgaca
    25621 ctcgtccgca acgcgcccaa gtatatcctg cttgccggtg cggatgcgga catcagccgc
    25681 agcctgctgg cgaaacgcta tccgcaggcg gtatttgaag aatacgattc ccgtgcggac
    25741 tttttggcgg ctgccgctgc cgcccgcaaa ggcggttttt ggcaaaggtt tacgggtaag
    25801 ggcgtggtgc aacactgcca atccccgatc gcgccgctgc ccgaagcgtg tgctgatatg
    25861 ttgtggtcga atctcggact gttggcggcg gaacaaatcc ttcctgtgct gcacaactgg
    25921 gcgtgcgcct tgaagacgga cgggctgctg ttttttacct gcttcgggcg cgacactttt
    25981 accgaactga aatgccgtct gaaagaaaac ggcattgaaa gccgcagcgt gatgtttccc
    26041 gatatgcacg acttgggcga tatgcttgct gaaaacggct tttacgaccc cgttaccgat
    26101 acggcgaagc tggtgttgga ttacaaaaag gcggaaacgt tttgggcgga tatggacacg
    26161 ctgggcgttt ggcgggcgat ggcgtggaac gatgaaaacg ccgcgcgttc gtgtgtcggg
    26221 acaatatttg agcgggaagg cggtttgggc attacgctgg aaacggtgta cggacacgcc
    26281 gtgaaaaaac tgatgctgcc gcaaggggag aacgtggtgc agttttttcc gaagagatga
    26341 tgtgcagatg ccgtctgaag ccgtttccag gtttcagacg gcatttgtct gtgaaaaccg
    26401 acagaaataa aggaaatgcc gatgtatagt g
//
LOCUS       DAWKST010000008        26355 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.008, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000008 DAWKST010000000
VERSION     DAWKST010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26355
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.008"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(418..493)
                     /locus_tag="WJL87_000224"
     tRNA            complement(418..493)
                     /locus_tag="WJL87_000224"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(458..460),aa:Asn,seq:gtt)
     gene            complement(586..945)
                     /locus_tag="WJL87_000225"
     CDS             complement(586..945)
                     /locus_tag="WJL87_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ7798717.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(968..1612)
                     /locus_tag="WJL87_000226"
     CDS             complement(968..1612)
                     /locus_tag="WJL87_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ7798718.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(1614..2150)
                     /locus_tag="WJL87_000227"
     CDS             complement(1614..2150)
                     /locus_tag="WJL87_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ7798719.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(2162..3259)
                     /locus_tag="WJL87_000228"
     CDS             complement(2162..3259)
                     /locus_tag="WJL87_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ7798720.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLASVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3262..4698)
                     /locus_tag="WJL87_000229"
     CDS             complement(3262..4698)
                     /locus_tag="WJL87_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEZ7798721.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQHADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4918..5700)
                     /gene="bioC"
                     /locus_tag="WJL87_000230"
     CDS             complement(4918..5700)
                     /gene="bioC"
                     /locus_tag="WJL87_000230"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ7798722.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFSMPSGKVHLTYHPLFFIARYSAAGRQ"
     gene            complement(5688..6335)
                     /locus_tag="WJL87_000231"
     CDS             complement(5688..6335)
                     /locus_tag="WJL87_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ7798723.1"
                     /translation="MKTKFYNHQGGHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKVSFEDYQQFPARPFDEIHQELTALFEMIGQDRR
                     ADLIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(6345..7487)
                     /gene="bioF"
                     /locus_tag="WJL87_000232"
     CDS             complement(6345..7487)
                     /gene="bioF"
                     /locus_tag="WJL87_000232"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ7798724.1"
                     /translation="MNVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEQLVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPPTVPKGTSRIRLSLTADMTMDEVRQFAVCL"
     gene            7710..8786
                     /locus_tag="WJL87_000233"
     CDS             7710..8786
                     /locus_tag="WJL87_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEZ7798725.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(8993..10459)
                     /locus_tag="WJL87_000234"
     CDS             complement(8993..10459)
                     /locus_tag="WJL87_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ7798726.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10657..11580)
                     /gene="speB"
                     /locus_tag="WJL87_000235"
     CDS             complement(10657..11580)
                     /gene="speB"
                     /locus_tag="WJL87_000235"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ7798727.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLTAPQVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11679..13571)
                     /gene="speA"
                     /locus_tag="WJL87_000236"
     CDS             complement(11679..13571)
                     /gene="speA"
                     /locus_tag="WJL87_000236"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ7798728.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYASTVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13738..14022)
                     /locus_tag="WJL87_000237"
     CDS             complement(13738..14022)
                     /locus_tag="WJL87_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ7798729.1"
                     /translation="MKKLLLAAVVSLSAATAFASDSAERQIYSDPNFEQNRVKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(14173..15981)
                     /gene="aspS"
                     /locus_tag="WJL87_000238"
     CDS             complement(14173..15981)
                     /gene="aspS"
                     /locus_tag="WJL87_000238"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ7798730.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALNVDLGDFPRMPYAEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIERTGTQ
                     NGDIIFFGADKTKVVNEAIGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKAAETKEA"
     gene            complement(16039..16740)
                     /locus_tag="WJL87_000239"
     CDS             complement(16039..16740)
                     /locus_tag="WJL87_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ7798731.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPK
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(16883..18031)
                     /locus_tag="WJL87_000240"
     CDS             complement(16883..18031)
                     /locus_tag="WJL87_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEZ7798732.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(18192..18455)
                     /gene="rpsT"
                     /locus_tag="WJL87_000241"
     CDS             complement(18192..18455)
                     /gene="rpsT"
                     /locus_tag="WJL87_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ7798733.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18758..19900
                     /locus_tag="WJL87_000242"
     CDS             18758..19900
                     /locus_tag="WJL87_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7798734.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
     gene            complement(21224..23968)
                     /gene="tbpA"
                     /locus_tag="WJL87_000243"
     CDS             complement(21224..23968)
                     /gene="tbpA"
                     /locus_tag="WJL87_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HEZ7798735.1"
                     /translation="MQQQHLFRFNILCLSLMTALPAYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKSSDTLSKEQVLNIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGVSQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDTNQYAHFVVEEECPNGYAACKDKPK
                     KDVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEHTQ
                     QTFDTRDMTVPAFLTKAVFDANSKQAGSLPGNGKYVGNHKYGGLFTNGENGALVGAEY
                     GTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGS
                     DKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGSN
                     LRHQDYYHQSANRAYSSKTPPKNGNKNISPNGSKEKPYWVSIGGGNVVTEQICRLGNN
                     TYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTLS
                     WNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAGIV
                     FKGDFGNLEASWFNNAYRDLIVRGYEAQIKNGKEEAKGDPAYLNAQSARITGINILGK
                     IDWNGVWDKLPEGWYSTFAYNRVHVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYDQ
                     PEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTIK
                     KHFTLRAGVYNLLNYRYVTWENVRQTVGGAVNQHKNVGVYNRYAAPGRNYTFSLEMKF
                     "
     gene            complement(24055..26184)
                     /locus_tag="WJL87_000244"
     CDS             complement(24055..26184)
                     /locus_tag="WJL87_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ7798736.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVSSEKPQAQKDQGGYGFAMRLKRRNRHPQAKEDKVELNPNDWEETGLPSKPQNLPE
                     RQQSVIDKVKTDDGSNIYTSPYLTQSNHQNGSTNSGANQPKNEVKDYKNFKYVYSGWF
                     YKHAESEREFSKIKFKSGDDGYIFYHGKDPSRQLPTSEKVIYKGVWHFVTDTEKGQKF
                     NDILETSKGQGDRYSGFSGDDGETTSNRTDSNLNDKHEGYGFTSNLEVDFGSKKLTGK
                     LIRNNRVTNATTNDKYTTQYYSLDAQITGNRFNGKAIATDKPDTGGTKLHPFVSDSSS
                     LSGGFFGPKGEELGFRFLSDDKKVAVVGSAKTKDKTENGAVASGGTDAAASNGAAGTS
                     SENSKLTTVLDAVELKLGDKEVQKLDNFSNAAQLVVDGIMIPLLPEASESGNNQANQG
                     TNGGTAFTRKFDHTPESDKKDAQAGTQTNGAQTASNTAGDTNGKTKTYEVEVCCSNLN
                     YLKYGMLTRKNSKSAMQAGESSSQADAKTEQVEQSMFLQGERTDEKEIPSEQNIVYRG
                     SWYGYIANDKSTSWSGNASNATSGNRAEFTVNFADKKITGTLTADNRQEATFTIDGNI
                     KDNGFEGTAKTAESGFDLDQSNTTRTPKAYITDAKVQGGFYGPKAEELGGWFAYPGDK
                     QTKNATNASGNSSATVVFGAKRQQPVR"
BASE COUNT         6558 a         6746 c         6197 g         6854 t
ORIGIN      
        1 aattaggagg tatttggggt gaaaacagcc gaaaacctgt gttggggttt cggctgtcgg
       61 gagggaaagg aattttgcaa aggtctcaat tagtatagtg gattaacaaa aatcaggaca
      121 aggcgacgaa gccgcagaca gtacaaatag tacggaaccg attcactcgg tgcttcagca
      181 ccttaaagaa tcgttctctt tgagctaagg cgagccaatg ccgtactggt ttttgttaat
      241 ccactataaa atcgagttta tcctagttgt ccaggacagc ccctattttt tcataaccat
      301 cacgaaagga attttgcaaa gattttattc catctcaaaa caatcatctc aaaaatgcgt
      361 ttctgaccgc cggtaaaaca aaaccctcta agaaaatact tagagggttt tgaaatttgg
      421 ctccccgacc tgggctcgaa ccagggacct gcggattaac agtccgtcgc tctaccgact
      481 gagctatcgg ggaataagcg cgaataataa aacagaaata ccgaacgtgt caatataaat
      541 tgacaatcga ttgcacttta tctgcaagcc cgcaatcttt atatgttata tggcttccgc
      601 aatatgcctt gccgcacgtt tggcatccaa taggattaaa ccaagtctgc cggtttcttt
      661 tgccaccagc accaacacgg catctttacc cgcctgactt aaaaggatat agcctgattt
      721 tcctttaatc atcacttgtt ccaattcccc gcaggcgagt tcctgcaccg agcgactccc
      781 caaagcaagc aaagtggcag aaatcgcccc taccctgtcc gaattcaaat gtgaaggaag
      841 cattgtcgcc atcggcaatc cgtcggttga gataacggca gacgcgataa tatccgtaga
      901 tgtattgttt aaatcttcaa ggattgaaat caataattgc tgcataaccc ctcctctctc
      961 ccaagtttta cacgcggttg ctgtaacggc ggtataaaat ccttaccaaa gtaacaaatg
     1021 cctctttgcc caaatcggga atgccgccga taaccaaaat aaatttggtt gaaccgatat
     1081 acaatgggaa aaatgtcaat tcgctctgac cggaaggatc gcaaacgccc caagcgttat
     1141 tgttgatata caggttgttc ttaatcagca gccggtattt cttttccatc tgtgcgactt
     1201 ctgccgccaa caaccccaac tcttccgccg cctcatgatg gaaattggcg ttggcaagat
     1261 acagaccgtt ccgatcgacc aataacgcct taccgctgcc ggacaattgt tccatcagca
     1321 acggcaattg ctcgtccgac aaattgatgc cgtctgaatg accgttttca tcgccataga
     1381 ggaattcgag tttttgcaaa cggtacaaca ggttcaaagc ggtatcgatg tcggcggtgt
     1441 ccgcccaggt aagcagcttc tcactgctga ccatttcgtc cgcatccgct ttcaacaggc
     1501 tgtgcaacaa agttttaccg gcactggggg catcgctgga tacggcataa aatgcaccgg
     1561 caggagtcag gcggggatat aaatttgctt gtagtgaaag tgttgattcc atattaaacc
     1621 tccagtcccg gatcaataga aaataacatt gcgctaacca attgttttac gtcatcttcc
     1681 ttacgggcat caatttcaaa aaccggaaca ttaagattat gttttgcaag atatttgtga
     1741 tacacgtcga taccgggctg agagcgtata tccatcttgg taataccgac aacgacgggt
     1801 gccttctcca gcagccctcg aaacgaatgt aaaaagaatt ccaaatcttt caacggattg
     1861 gttcgggcat tatctaaaag caagaccaaa cccatactgc cttggcttaa gatttcccac
     1921 ataaagttga accgttcctg accgggcgta ccatataaat ggactttggt atcctcatcc
     1981 aagctgatgg ccccgtagtc catcgccact gtcgtattcc ttttcctatc caaagtcata
     2041 tcggatgcgg aagcatcggt ctgaacgagt gcttcgtccg aaatagccgc aatggcagtg
     2101 gttttcccta cgccgacagg tcctgtgaaa ataattttat tttctctcat ctctccgcct
     2161 cttagctgcc caacagtttt ttcatcagcc tttgcagaag gccgcgcgac tgggattggg
     2221 atggtgttgt gattttttcc gcttttttca tcatttcact atcagaggca gaatcggctc
     2281 cgatatttat tttatccgcc atatcggagt atgcctgttg tgaaaccgtt tttaaatcta
     2341 ccgacaaaaa cccggttgta taggttgccg caagataatt cagaatatca ttgaggttta
     2401 aaggcatcac tttatacaac acgttaaggt tgacggatgc cttggtcaga aatgccgaca
     2461 agcgtatcga ccccggcaca cttgccaacc gggtcaggtt tggccaagat ttcaacgtaa
     2521 acggagtatc gggagaaatc ggataaatca acctgccctg cgccgtccaa atggaaaact
     2581 gccacataca ggacataatg cctactttag ccttttcgcg ccattgcggg ttatcgggaa
     2641 cagtcttgca gctgacctgc aaattttcgt ctttgcacaa ttcttcgagt ttttgcacac
     2701 tttctgtcag caaaacccgt tgtatcgagg ggaaaacaat aaggaccggc ttatttccat
     2761 gcaagatagc gatgtcctgc ctgttctttt ccgcaaaccg caacgccccc aataatcctt
     2821 tattcgggtt aaactggcgt atcgtaaccg tacgctgcac attcccgtta tttttcgccg
     2881 accggtctgc gggtgcaata aacgatttcc cataaacatt ctcgccctgc aacaatttgc
     2941 ggagcatagg aaacaatgtt tcaaaccgaa tcggtttggg caggtaggga acttcagaat
     3001 cgggaacttt ctccgaacag acggcgacgg gtatatcctt ataacgctcg gcaagctctt
     3061 tccaaagttc aaaaccgccc tcggcatcgg tatccgccaa aaccaaatcg ggcacggcac
     3121 tgccgtctga aggggatact gtttcataac gggtggtatt gtgcattttg aatgccattt
     3181 tgaaaacgga ttcctgctgc gccgtcatcc ccgccaacat tacgcgtact gttttaattt
     3241 tcggcagttg aacttccatt tttattttcc gtaccgtttt atttttttaa tattttgatt
     3301 catacgctgc agcagccggc tcatcagcat aacgacctct tcaggaagcc tgtccgcacg
     3361 ttccctcaat acccttaaaa actgccccaa cctatcccaa tcttcagtac gttcataaat
     3421 atcgatcaac gtaatataaa gctgggactc gtcgggatat ttcaataccg cctgctccaa
     3481 cacatccatt gccgcttcaa tctgaccata catcagcaac gactctactt ctttaaccgc
     3541 atcgtctgcc ggagacgaac cggtgttgat caacgaagaa tcttgaagca ccaaatcccg
     3601 atgttgcgat ttgaatttct gtatattttt cggcagatac ccgtgcccca taccgatgtc
     3661 tttgatttgc cggtcgttcg gccctttttc caaatcatcg aaaacttcat ggtaacccaa
     3721 gctgtacccc caccccagca tccgctcttt aacctgcctg ccgtagttgc ccaacgtttg
     3781 gtaaagtttc cacaaatgtc cggcaaaacg gtctatgtcc gcgtgttggt aatcgagttt
     3841 caacgcatcg ataatcaggt ttgcaggttt ttcggaagtt tggatggcac ggtcgtattg
     3901 tttcgatgcc gtttcatagc tgactttgtc tttaaggatt ttcgcacctt gatcggcacg
     3961 gaccaaaccg gcaatcgcac cgatttcctc ttgactgatt ccggacacgt cttttttgcc
     4021 ccgcacaatc gggatgcgct tgatttcttc ggcttcataa gccttaccgc cggcatccgg
     4081 cgggggcgat gcggaagctg ccgtcgcaga accgccttcc gcacgttttt caatctcctg
     4141 agtaccccat cccaaacctt cttccgccaa gacgcggata cgcaaatggt ttgaatcgcg
     4201 ctgtaatgcc tgttcgatat attttgccaa aagttcggaa ggaatcagtt tgccgtattt
     4261 ttgcagattg tctgccaaaa catcgacatc ccccacttcg agattgatat cgagcagctc
     4321 gcggataagg ttttcaggtt tcgcttcacc atccggaatg ccgtccagat aggcagccaa
     4381 agattcggca gccttgccct gataaccgaa ttgcttataa acctgatact ccgtaagcgg
     4441 atcgacttct tgcgcggata cggcggcgga tgcggtctcg gcactttcgt tccaagacca
     4501 gtcgggttgc gtgccgtctg aaaccgattg tccaatctga tcgacccagt ccgaatcatt
     4561 gcttccgggt gtctgcccct gcgtattgcc gacaccttgg gatttgcgct tcggtgtttt
     4621 cttaccctgc ttcgcacgca taaccaaaag catcagcaat accgccagtg ccaaaccgat
     4681 aattaatgag ttttccaaag gatactcccg ataccgtaac gagcggatag ctgaccgcct
     4741 gcaacggcca ttgtccgctt ttattattaa cgatttactg aaatagactt gtaagtttta
     4801 aatcatacca taatttaacg tttaacaata tgccttctgc acaagcctcg ccatattacc
     4861 ttttacccac atcagtatca atacccgata taaaaataac tttgcccata agctgcctta
     4921 ttgcctgccc gccgcagagt agcgcgcgat aaaaaataac ggatgataag tcaggtgcac
     4981 tttgcccgac ggcatactga acgccgacaa ataatcgcca ataaatccat tgagattttt
     5041 tcttgtccaa tttttttggt tcgtggctgt tacgcctgta tatttaaggt gtttgagtac
     5101 atctaacggc gtatcaaagt ctagtattac cttaaaatcc tcacaccata aaagctcaaa
     5161 atctttggct aaccaagtct gccattggga taaagtcggg taatttaagc ctatatttgt
     5221 aatttggcgg acttctttta aattgtcttt gccaaaggtt gcaaccgcca ataatccgtt
     5281 tgctttcaag cctgttttgc aatgggcgat aaaagcgtcg ggttgatgaa accattgcac
     5341 ggcagatgcg cttgcgatta agtcaaattg tcgttgaaaa ggaaagtttt ccgcatcgcc
     5401 gcaataaaaa tcaaaggatt gcggcagttt ttcagccagt tggggctgca catcgcacaa
     5461 atcattaaat aaccaataat tcgctgaaat ctgtttttgc agcaaggcac tcaacattcc
     5521 tgagccgcag cccaattcca acacgttttc caatggcata tccggcaaat aatcttgcaa
     5581 atgcgccatt aaattaatcg tcattttttg ttggattaag gcgtgccggt cataatcgtt
     5641 taatgctttt tggaaagcct gccgaatgcg cgatttattt atggcagtca gtgattccat
     5701 agtgccgacc aatgggtgaa tcttgaaaac aggtaatgtc cgccgtcaat ttcccgaacg
     5761 gtgcaacgcg gtgtccaata tcggtgctga ttggcaggca taaaaatttt atcgcctgat
     5821 ccgaccaagg catttgtcca gcgaatcaaa tctgcgcgcc tatcttgccc gatcatctca
     5881 aaaagtgcgg taagttcttg atgaatttcg tcaaacgggc gtgcgggaaa ttgttggtaa
     5941 tcttcaaaag atactttatc gccacacatt ctgcgttcaa attttaaacg ggtgttttcc
     6001 gtgaggttct ccaatgtgcc tttaaaaacg gcgcaaggga taccgaaatt atcatcgcaa
     6061 ggcaaacctg tgccattcac tgccgttgcg gattttaatc ttattccttg caatgccctc
     6121 tctgccgccc aaacgcccat tgaccacgcc accaaacgga tgtgccgata ggcggaaaaa
     6181 tcaaaatcca aatttaaatc ttgataatcg tagcaaatca ataaatcgtg attttccggc
     6241 aaaatcaaat gatttacagc atcgggcggc gttccccaac ctgcaaaata caggattaaa
     6301 tgtccgcctt gatgattgta aaattttgtt ttcatatcat atccttacag gcacaccgca
     6361 aactgccgca cttcatccat tgtcatatct gccgtcaaag acaatcgtat tctggatgta
     6421 ccttttggta ctgtcggtgg tctgatgggc aggcaataat aaccctgcct ttgcaggtat
     6481 tccgctttgg caagggtggc ttcattcccg cctaaaatat aggggacgat acaggtttgg
     6541 ctcggcatta tttgcgtccg atgcgccact tcccgccgta aaaatgcgct taactgctca
     6601 agatggcttc tttcttttga gaattgcggc aatcgttcaa aaataaaata agtccaagcc
     6661 acattaaacg gcggcaatgc ggttgaaaaa atcaatgggc gcatttgatt aatcaaacat
     6721 tctttcaata cttggttgca gacggcatac gcccccaccg aggctaaggc tttaccgaaa
     6781 gtgccgacca ataaatcaat ctcggcaatc aaattatccc gttcggcaat ccccaatccg
     6841 ttttgcccat aaacaccgat tgcgtgggct tcatccacat aaagataagt attgggaaac
     6901 tgttttttta attggacaag ctgtttcaaa tccgccacat cgccgtccat actgaaaaca
     6961 gattcggtaa cgataaaagt gcggtcaaat tttccgacgt ttttttcaag cagatttttc
     7021 aaatgttcat aatcattatg acgataacgg aaaaacgcac accggctcaa acggatgccg
     7081 tcaatcatac tggcgtgaac aaatttatct gccaaaatca aacttttcgt cgtcgtcaaa
     7141 gcaggcaaaa taccgagatt ggcgtgatag ccgctgttga acaataacgc gctttcccgt
     7201 tggaaacgtt gtgcgacaag ctgctcaaga tcggtataaa taggaaagtt gcccgttaat
     7261 aaacgcgatg aagaactggt aaaagaggga aaattaccgc cgtattgctg caaaaaagac
     7321 cggcgcaagt tttcatctga tgccaaaccc aaataatcat tagacgacat attcagcatt
     7381 ttgcggtttt cccgcgtaat ataccgccct tgatgaatca aatccggaat cgaacgatat
     7441 tggttttgcg cgccgagttg ttcaagctgt tgtttaaaaa cattcattat gatgtaaata
     7501 ttcctgagtt aaagcctgaa ccaacccagc atcaaacaat caagtaatgc ccttttaata
     7561 aaaaccgttt tgccgccgac ctttgccaca cccctgcttt cataatccaa ggcggaaatt
     7621 tccgcgacat ttgtttccgt tgccattacc caatccgtcc gcactgtacc gaagcgcata
     7681 ttttcgcaca aattggtacg gtttcaggta tgatttgccg aaaacctatc ggacacacac
     7741 catattttga tatgaataaa acaatctgcc gcactgctgc actattgata agcggttttt
     7801 catacgcaaa caccgtcatt cccgatgtct cccccgtcgc acaaggtcaa catgtcttca
     7861 ttaacatccc tcagcaacgc ctgttcctct acaccgacgg caaactgacc aaggtttatc
     7921 ccgttgcagt aggtcgggcg atgacgcaaa ccaatctagg cgaacataaa atcggtgcga
     7981 aagcctataa ccctgtttgg tacatcccca aatccataca gaaagaacgc ggcgacggcg
     8041 taaaaaccat agccgccggc ccggacaacc ctttggggcc ggtttttgtc cgcttaggcg
     8101 accccaaact cggtttgggc atacacggga ccaatgcgcc ggccagcgtc cccggggttc
     8161 ggagtcacgg ctgcgtccgc atgaaatcgc ccgacgcgct cgagtttgcc aaaaccatcg
     8221 ccagcggctc gcccgcctcc gtcatctatc aaatggcggg tctcaatgaa gatgcggatc
     8281 gcaacctgtg gcttgccgcc ttccgcgacc cttacggtaa gaacaacctt gacatcgcct
     8341 ctctgaaaaa aagtattgcg caatgggcaa aaacacaggg taaaaccatc gcgcccgaga
     8401 aagtcgatgc tgtactcaaa gaccgcaccg gatcggccgt ctgcctgacc tgcggcaaaa
     8461 acggcaagat gaagatgccg ctcaaatcgc tggcgtggat acagggttct tcctcataca
     8521 gccaagctga agtcattgaa caaactgagg aaaccaactc tgccgaaata tccgagacac
     8581 gcacgcccga agtgcctgat gtacacacgc ccgaggcaca accgcattta aatacccaat
     8641 ccgacggcac gccgactgcc tataccgaac cggctgccga ttcatcgccg caagtagaaa
     8701 cacctgatca ggctgcttcc gagccggttg atgtattatt ttcaatagat gtgatacggc
     8761 agggaaattt gcgtttaggt aattgaataa ccttctgatt atttaatgct tatgtttatc
     8821 agaagttgat aggcggtttg gtttttgttt ggcttttcct acaccgccgc ctgccgcttt
     8881 ctgcaacatt caagcgcaca aatatgccat ctgaaggctt tagacggcat atttcacgat
     8941 aatagtcgcc caatcaaata aacacagatt ggaaatttat tttatttttc ccttaccaat
     9001 accccagaac tttccaccaa atgctgccga taacgaaaaa aatcagaaaa ttgactacgc
     9061 tcatgataaa acccgccttc caccattctc ccattgtggt gtagcccgaa ccgaaaatca
     9121 caggcgaagt accggtcgca taatgagtga gggtcatcat aatgttagat gcggccgcca
     9181 tcatcagcgc ggtcggcatc gccggggcat tcagtgaaac ggcagcagcg aaaaatgcgc
     9241 cgaacatagc ggtaatatgt gcagtagtac tggcaaacat ataatgcgca tacatataag
     9301 caagcacgag gattacgccc gcagccgtgc cgctaacgcc caaaccgccg acactttccg
     9361 ccaacactcc ggagaaccat ttaatcagtc cgagtttatt taaaaatgcg gccatcataa
     9421 tcaatgcgcc aaaccaaata atcgtatccc acgcactttt ttctttcaaa acatcgtccc
     9481 aagtcaatac accggaaagc aaaagcaggc ttaatccgat aaatgcggtg gcggtggcgt
     9541 tgatactaaa agcgtgattg ccggtaataa gggcgggaac atctgcccac aacagcagca
     9601 agataccgaa aatgaccgcc ataatgattt cgtctgccga cattttaccc atctccctca
     9661 gacggtcttt ggcaaattga acggcattgg gcgtttcttt aatttcaggc ggatacaaaa
     9721 aatataaaat caaaggcata acgaaaaagg cgataacgcc gggaacagcc attgcccacg
     9781 cccacgcccc ccaagaaaga cggaaactac tgcctaaatt ttcggcaatc aagttgacga
     9841 ttaaagggtt gggggcagtt gcagtaataa acatagccga cgaaatggga ttggaatgat
     9901 agttgaccaa agccaaatat ttacccatct tgccttctgt gccttttgcg ggattggagc
     9961 cgtaactgcc ggcaatcgac tgcataatcg gatgtataat gccgccgccg cgcgcggtat
    10021 tggaaggggt aacgggagcc agcagcagtt cggaaagagc gagactgtaa ccgatgccca
    10081 gcgtttttct tccaaaaacg gcgataaaca aatatccgat acgcatcccc agccctgttt
    10141 tgagcaaacc gcgcgaaatc ataactgcga tggcaatcag ccaaatcaac ggattggcga
    10201 acgcactcaa cgcatcgctc atcgccgcgc ccggtttgtc ggcggttacg ccggttactg
    10261 cgaccaaccc gacggcaata atcgacagcg cgcccaacgg catggccttg ccgataatgg
    10321 cggcaatcac accgacaaac atggccagca gcgtccaagc ctgaggcttg accccgtcgg
    10381 gtacgggcag tgccaaaacc agggcgcaca atactgcggc aatggcgagg ggtatcggtt
    10441 tgaaacccaa tttcatcata ttgacctccg taaaaaagac cgtcccgaaa aatcggaaaa
    10501 ataatattta actaattgtt ttataatata tattcagata tttcaccgtc tttccgatat
    10561 gcggctccgg gcaacttttg tttcagtatt tgaattttca ttagactgaa tacgccgttt
    10621 gaacggcacg gcgaaaaccc gggggatgcc ggacgttcag tcctttttcg caccttgaag
    10681 gtaaagcatt tccaaggcaa ttgtggcgcc ggccaaagcg gtaatgtcgg attggtcgta
    10741 agagggggca acttctacaa catccatacc gacgatgtcg agatccgtca gcccacgtag
    10801 gatttttaat gccctgtcgc tgctcaagcc gccgcatacg ggcgtaccgg tcccgggggc
    10861 gaacgacggg tcgaggcagt ctatgtcgaa agtcaggtaa acgggcatat tgccgacggt
    10921 ttctttgatt ttacggacgg tctcttcaac actgtcttca ttaacttggg gggcggtcaa
    10981 cacagtaaaa ggcaattttt tactgtgttc ggtacgtatg ccgatttgta cggaacggga
    11041 cgggtcgatg aggccttcct tgggggcggt atagaacatc gtaccgtggt cgtattcgct
    11101 gccgttgtcg taggtgtcgg tgtgcgcgtc aaaatgaatc agtgcgagtt tgccgaaata
    11161 gcgggcgtgg gcgcgcaaca acgggagggt aatgaaatgg tcgccgccca aactcaaaca
    11221 gcgtttgccg gaagaaagta atttgccggc gtgcgcttcc attttttcga caaaatccct
    11281 gctgtcgcca aaagaaaaaa ccaagtcgcc gcaatcaata atgttcaggc gttcgcgcac
    11341 atcaaatgtc catggaaacc tgcggtgctc ccaagcgagg ttgacggagg cgcgccggat
    11401 ggcttcagga ccgaaacgcg cgccggaacg ccctgaaacc gccatatcat aaggcacgcc
    11461 ggtaataacc caatcggcat gactttcata cggcataaaa ttaagcggca ggcgcaaaaa
    11521 cccgaaatta ttggaaacga gggagttgtc ggtttgtcct gccagtgtgc tgtattgcat
    11581 cgtaatgatt ccttgtaatt ggtttcaatc ggtcgtgatg attggtgttt gagtaagaaa
    11641 atcgggcttc agacgacata tccgatgcct tgatgcgtct attcgtcttc caaataggta
    11701 taaccattaa gccccgcttc gagttctttt aagaaagaca tagcctgcga ggcaggaagg
    11761 tctgaatgtt cgatttgttc gcgatagcgt ttcatcagct ctttcggatc ttgataaacg
    11821 tattcgagca tatcggcaac ggtgtttcct tcatcgtaat cgatgacggt aaattgtccg
    11881 tcttccccta caacaacatc ggcagtggca gtgtcgccga aaagattgtg catattgccg
    11941 agtatttcct gatatgctcc caccataaaa aagcctaaaa gcggcggctc ttcttcggga
    12001 taatcaggca taggcatcgt accggcgatg ccgtctccgt cgatgtagtg gtcaatcgta
    12061 ccgtctgaat cgcaggtaat gtccaacaac acggcgcggc gcgcaatcgg ttcattcaaa
    12121 ccggtaatgg gacaaacagg gaaaagttga tctatgcccc aagcatcggg caaagattgg
    12181 aagagtgaga aattgacata cagcttatcg gcaaaacgtt cttgcaattc gtcaataatg
    12241 gttcggtgag accggtgttt ttcattaaac aattcgccga cttcatgaca gatatttaaa
    12301 tacagttgct ccgcccacgc acgttgcgcc aaactcaaca gcccgacatt atactgatta
    12361 tgcacatcgg caagatcaaa ctgcccttcg tgtatccagc tgcgtaagga acgtttttcc
    12421 cgcgaggcgg aaatatccgt ccaagtttcc cacatactgt gcaacacacg cggtgcttcg
    12481 ggcgatggcg catccagccg gcgcggtttg taacgttcaa cgcctataac attagcaacc
    12541 aaaacggcgt gatgtgcggt aatgccgcgc ccgctctcgg taatgattgt cggatgcggc
    12601 agcccgtgtt cgagacaagc ctggctgatg ccccatacga ctgtggaggc atattcgttg
    12661 aggctgtaat taacggaaca atccgattgt gtgcggtttc cttcgtaatc cacgccaagc
    12721 ccgccgccta catcaaaaca gcggatattt acccccagtt tgtgcaactc aacataaaac
    12781 cgagccgatt cgtgtacacc tgtggcaaca tcacggatgt tcccaagctg cgagcctaaa
    12841 tggaaatgca aaagctgcag gcagtccagc ctgttttttt gtttcaaaat atcgaccagt
    12901 tgcaaaactt gggaagccga caagccgaat tttgattttt ccccacccga agactgccat
    12961 tttcccgaac cttgggaagc cagtctggcg cgcacaccca aacggggctt gatgccgagt
    13021 ttttccgcct cttccaatac catttgtatt tcggacagct tctcaatcac caaataaacc
    13081 tgatgcccca gtttttcgcc catcaaggcg aaacggatat attcacggtc tttatagccg
    13141 ttgcagacga ttaatgtttg ccgggtgccg gcgtgtgcca aaaccgccat cagttcggct
    13201 ttagaaccag cttccaaacc atgcggttgt ccgcttgaca taagcgattc gatgacgcgg
    13261 cggtgttggt tgaccttgat agggtaaacc aaacaataac cgcccttata gccgcactct
    13321 tcccgtgccg tctgaaaggc gcggttaatg tcgcggaggc ggtgttcgag gatttgcgga
    13381 aaacaaaaca aaacaggcag gcgcgcctga tgtttttgtt gcacggcttc agtcagtttt
    13441 tgcagtgaaa cagtttgatt gtgttgcgag ggattggggc ggacgatgat ttcgccggaa
    13501 tcgtcaacat cataataacc tatgccccaa tgattaatgt tgcacacttc acggatggta
    13561 aggataggca taataaacct gctccgtctg tcgtgttgaa aaggaatgat tataacaaat
    13621 cagcgtgaaa tgtcattttt ttaataagaa aagcctgcct catacctgat gaggaacagg
    13681 caaaatgccg tctgaacgct tcagacggca ttttggttca tctttccatc aaaggagtca
    13741 gcgatcgagc tgctctttga tgattttcag gtcggggtaa gacaacacga tgtcgtattc
    13801 gcggccgtct ttataggctt ccacttccag cacaggctta ccccaatggt cgtcggcatc
    13861 gacatcgtga acctgataac cgcgctgttc caacattttc acagctttta cgcggttctg
    13921 ttcaaaattg ggatcactgt aaatctgacg ctcggcagag tcgctggcaa atgcggttgc
    13981 ggcactcagg gaaacaacgg cagccaataa cagttttttc attttcagtc ctttctttat
    14041 cggttgattg aataagatgt gtttttcaat gccgccatta aaacacagca aaattaggtt
    14101 tgaattagag ggaagtcaag aagcgtaaat gccgtctgaa aaaaacagag ccgtcaaacg
    14161 gctccgtttt ccttatgctt ctttggtttc agccgccttt tggcgcaggc gcaggctcaa
    14221 ttcacgcaac tgcttgtcgt ccacgctgtt gggcgcgttg gtcagcaggc attgggcacg
    14281 ttgtgttttc gggaaggcaa tcacgtcgcg gatagattcg gcgccggtca tcagggtaac
    14341 caaacggtcg aggccgaatg caaggccgcc gtgaggaggc gcaccgaatt tcaggttgtc
    14401 caagaggaag ccgaatttct cttgttgctc ttcagggctg attttcagcg cggcaaacac
    14461 tttctcttgt acgtctgcac ggtggatacg gatagagccg ccgccgattt cccagccgtt
    14521 caataccatg tcgtaggcac gggccaggca gtttgcaggg tcggaaacca tcaggtcttc
    14581 atgaccttct ttaggcgcgg taaacggatg gtgtacggca acgtagcggt cggcttcttc
    14641 gtcgtattcg aacattggga aatcgacaac ccacaaaggt ttccattcgt ctgtgaagta
    14701 gccgttgtct ttgccgtgtt ccaagccgac tttgatacgc aatgcgccga tggcttcgtt
    14761 cacaactttg gttttgtccg caccgaagaa aataatatcg ccgttttgtg tgccggtacg
    14821 ctcgatgatt tctttcaggg cattctcaga caggaatttt acgattggag attgcaggcc
    14881 gctgtcttcg ccgtttgaga ggttgcccac atcgtttact ttgatgtatg ccagaccttt
    14941 cgcgccgtag atgccgacaa atttagtgta ttcgtcgatt tctttgcggc tgaattttgc
    15001 gccgttcggt acgcgcaggg cgactacacg gccgcctttc atatctgccg cgccacggaa
    15061 gactttgaat tcttccgttt tcatcaggtc ggtcaactcg gtaaatttca agttgatacg
    15121 catatccggt ttgtcagagc cgtagtagaa catggcttcg gcgtaaggca tacgcgggaa
    15181 gtcgcctaaa tctacgttta aagcatcttt gaagacttgt ttcgccatac cttcggtgat
    15241 gtccataatt tcatcctcgt tcaagaacga ggtttccaag tcgatttgag tgaattcagg
    15301 ctgacggtcg gcacgcaagt cttcgtcacg gaaacatttg gtaatttgat agtaacggtc
    15361 gaaaccggcc accatcaaca gttgcttaaa taattgcggt gattgcggca gcgcgaaaaa
    15421 ctcgcccgga tgaacgcggc tcggcacgag gtagtcgcgc gcgccttccg gagtggagcg
    15481 ggtcagcatc ggcgtttcga tgtcgatgaa gccttgcgcg tccagataac ggcgaacgcc
    15541 catagcgact tgataacgca ggcgcaggtt gcgttgcatc accggacggc gcaagtcgat
    15601 aacgcggttg gtcaggcgaa cgttttcgct gatgttttca tcgtcgattt ggaacggcgg
    15661 cgtggcggcg gcgttcaaga cttcgatttc tttggcaagg atttcgattt tgccggaaat
    15721 cattttgtcg ttggtcgtac cttcgggacg gttgcgtacg cggccggtaa tgctcaaaac
    15781 gtattcgttg cgagcggaat cggcagcggc aaacgcttcg ggcgtgtcgg ggtcaatcac
    15841 gacttggacg atgccttcgc ggtcgcgcag gtcgataaaa atcacaccgc cgtggtcgcg
    15901 ccgacggtgt acccagcctt tgacggtaac ggtttggtct aagtattgct cactgatcag
    15961 accgcaatag ttggtacgca taaaatcacc ttttattgat ttaaactgaa aacagaaaat
    16021 gccgtctgaa cggcggcttt attgttgttc gggcaaatcc gccttttcag acggcatagg
    16081 tcctgccaat gttttgacgg gcaggtcgtc agggatgacc atacccagcg aaatcacata
    16141 tttcaacgct tcgtccacgc tcatatcgag ttcgcgcaca tcgcttttct ttaccataat
    16201 atagtaaccg ccggtcggat tcggcgtggt cggaacatac acggaaagat aatcgccgtc
    16261 cttcggcaat gcggccttaa ccgcattcga cacctgaccg gacacgaatg cgattgtcca
    16321 aatacccgat tggggaaacg gcacgagtac tggtgtttta aacgaacggc tgctgtcgga
    16381 cagcaacgat tcggatactt ttttcacact cgaatagatg gacttcacaa ccggaatccg
    16441 ccccaacaag ctgtcccacg cggcaagaat ctgccggccc aatacgtttg cggcaaacaa
    16501 tccggttaca aacaatacgg caatggcaac gataacgccc agccccggga tattaaaccc
    16561 caaaacatat tgcggccgcc attgcttcgg cagcaggttg acgagctgat cggacgcgga
    16621 aacgatatag gaaaccaccc aaaccgttac cgcaatcggc agccagacca aaatgcccgt
    16681 aatcagatat ttttttaacg ccttggcagc tttaccgcct tcggccgcag gttccgtcat
    16741 cttgcttgat tccgacaaag tccgtacaaa ccgcacatta tacgcgtttg cccggattca
    16801 aacgaaattt ttatcccgcc ccgccaaacc gccggcggct tcagacggaa cggcaacttg
    16861 atataccgtc tgaacacgcg gttcagatgc cgtccaagtc gttgaacatc aacccgatac
    16921 cgataccgtt ctgcttgtgg ttgtagtcga tcaggctctc gccgtaaccg tggaatccgc
    16981 gtaccacgcc tttgagtttg cccttaatcg gaaacgtgta ggcggcttca atcgcgccgt
    17041 agcccgtttt ggggttgtag cgcaatacgg aatacacatt ctgcctgtcg ttcaggcggt
    17101 actgcagctt cacgtcgcca taccccatat agtcggcaat atcgggattg tcgtttttat
    17161 cgccgctctg atcgaacgca cgcacccaca cgcgcggaat caccgtcaat ttgccccatt
    17221 ccatgcctgc catggcgtaa atcctgttcc acgaacgcga ttcgggacgg ctctgtccgt
    17281 tggactggtg gacaaaaccc gcaccgagca tacgcagcct gccgccgaac ggcaaatccg
    17341 ccttcacagg ctgggtcagg aaaatttcag gtttgtaatc cgtattgcgg aacggcgcgg
    17401 atttcctgcc ttggttgtaa atctgccaat cggatctttg ggtgtagccg aaccacagat
    17461 ccgcgcgggt tttaaacaaa tcttcggcaa ttttgctttt gaacgaaacc tgcaatttgg
    17521 tttccgcacg tttctgctgt ccgaattttt cctgtacagt cgtaccgcgc gtcggcgaac
    17581 ccggggcata gttgggcgaa ttgttgtacc agagcggcat aaggtacatc ggattgtgtt
    17641 cgcgtacgcc caacagcccg cgcaaatcgt ttttgtccaa gtcgtacatc aggctcaaag
    17701 gcgtatagat gtcggcggtt tcgcccgcac tgtcggcagg aagcgcatcc ccgccttttt
    17761 caacaacaat gaccgcctcg cccttatcca ggctgctgcg gacggtttcc gtcagattga
    17821 gtacggcttt cgactcctgc ccttcctgcc ctgccgaaga cggaagctgt gccgcaaaaa
    17881 tcctgtcgta acacgccaaa cgcgtaacat tgtccgtcaa agcggcgcat tgcagcgcgg
    17941 tctctccaaa agcggatgcc atcggcaaca gtcctgtcaa aagaatatag cgcatattcc
    18001 gtgtattcat ctccgccccc attgtcggca tattggtttt caaacggcat tttatagcgg
    18061 attcggataa aaaaccgcac cctttccgcc atttcggtat ttagcccccg taatacagaa
    18121 aaccccgaaa ccgtcaggct tcagggtttt ccgcttatcg cgtatcaacc gccttggcgg
    18181 ttttgcaaaa atcaagccag tgcttttact tttgcagaca aacggctttt gtggcgagcc
    18241 gctttgtttt tatggaacac gcccttgtcg gcgatgcggt cgatgacttt gacggactct
    18301 tggtaaaccg cttgtgcggc agctttatcg cctgcttcga ctgctttcaa tacttttttc
    18361 actgcagtgc ggaatgcggt acgcaggcta gcgttgtggg cgcgttgttt gacggactgg
    18421 cgggcacgtt tgcgtgcttg tgcgctgttt gccatattgg aatctcctgg aagaaaaata
    18481 aattctgtaa acgcgcaatt ttaaaggggg tttacattat atgcaagcct tttctctcta
    18541 tccgcccgtc ggacaccctg acagcgcaaa aaaacggcaa aatcgtgcgg acgttttccc
    18601 cctcttttga aaacagggta attttttagg gaaatcagtc caatatcact ttgagtttgc
    18661 acaaatttac tttacaatgc ccaaaccttg cgccggggta acgatggggc gcggggattt
    18721 ctttttttcc aacctctttc atcaggaatc tgcccgtatg aaaaaatccg tattagccgt
    18781 tttggccgcg ctgtctctgg cagcctgcgg cggcagcgaa aaaaacgccg tgcaacctca
    18841 agcagacgct gcgtctgctg ccaatgccga agccgctgcc accgatactc tgaacatcta
    18901 caactggtcg aactatgttg acgagagtac ggtcgaagac tttaaaaaag ccaacaatct
    18961 gaagctgact tacgacttgt acgaaaacaa cgaaacgctg gaagccaaaa tgctgaccgg
    19021 caaatccggt tacgatttgg ttgtgccggg catcgccttc ctgccgcgcc aaatcgaggc
    19081 gggcgcgtat caaaaagtca acaaagacct gattcccaac tataaaaaca tcgatcccga
    19141 actcttgaaa atgctggaaa ccgccgaccc gggcaaccag tatgccgtcc cctatttctc
    19201 cggcgtgaac acgattgcga ttacggcgaa aggcaaagag cttttaggcg gcaagctgcc
    19261 cgaaaacggc tgggatttgc tgttcaaacc cgaatacacc cacaagctga aatcctgcgg
    19321 catcgccctg tgggacaccc cgagcgaaat gttcccgatt ttgctgaact acttgggcaa
    19381 agaccccaaa ggctcgaatc ctgaagattt gaaggcggcg gcggaagtgt tgaagtctat
    19441 ccgtccggat gtcaaacgtt tcagcccgtc catcatcgac gagctggcgc gcggcgacat
    19501 ctgcctggcg gcaggcaacg gcggcgattt gaacttggcg aaagcacgtt ccgaagaagt
    19561 gaaaaacaac gtcggcatcg aagtgctgaa tccgaaaggc atgggcttct ggattgagtc
    19621 ttggctgatt cccgccgatg cgaaaaacgt cgccaatgcc cacaaataca tcaactacac
    19681 gctcgacccc gaaatcgcgg cgaaaaacgg catcgccgta accttcgccc ccgccagcaa
    19741 accggcgcgc gaaaaaatgc ctgccgagct ggtgaacacc cgttccatct tcccgaacga
    19801 gcaggatatg aaagacggtt tcgtgatgcc tcaaatgagt acggatgcga aaaaactgtc
    19861 ggtcagcctg tggcagaaaa tcaaagtcgg caccaactga tttgaagcat taaaaatgcc
    19921 gtccgaacat cgttcggacg gcattttata ttggattgaa atagaaatat ttatatagtg
    19981 gattaacaaa aatcaggaca aggcgacaaa gccgcagaca gtacaaatag tacgaaaccg
    20041 attcacttgg tgcttcagca ccttagagaa tcgttctctt tgagctaaag cgaggcaacg
    20101 ccgtactggt ttttgttaat ccactatacc gtcgttccca cggtcaggat ttcagattgc
    20161 ggacatctgt cagaaaagac aaaaaacctt ccgccgtcat tccctacagg cgagaatccg
    20221 atccgttgaa attcggttgt tttaaataaa ttcttgcagc tttgattttc tgtttttccg
    20281 ataacgccgt aactttgaaa cgcgaaagcg gtaatccaat ccgttgggat tttgcaactt
    20341 caaatcaatc cgcaaactga aatcccgtca ttcccgcgca gtcgtgaatc cgaacgcgtc
    20401 cgcacgaaaa cctgcatccc gtcattccca cgcagtcgtg aatccgaacg cgtccgcacg
    20461 gaaacctgca tcccgtcatt cccacgaaag tgggaatcta ggacgtaaaa tctcaagaaa
    20521 ccgttttacc cgataagttt ccgcaccgac agacctggat tcccgcctgc gcgggaatga
    20581 cgaaatttca gcgagccgta gggtgggctg tagggtgggc ttcagcccac caatttcacc
    20641 gtatcaaagg tttttgggaa tacggcggtt tcggttttcg gtgggaatgg gcggaatgga
    20701 taacattgac ggagttgggg gaataggtgg aaacggtggg attggtgggc tgaagcccac
    20761 cctacagcca gccctatgtc cacgctgcac atcctacgcc cgccttgcgt ttcttataag
    20821 cagcatttta cgcaatactt tgcgtccgct gaaaaaattt caaactgtct tttttatcaa
    20881 atgcgaaaaa tataacgtca ttcccgcgca ggtgggaatc tagtctgtcg gtgcggaaac
    20941 ttatcggata aaacggtttc tttagatttt acgccctaga ttcccgcctg cgcgggaatg
    21001 acgggatttt agatttctgt ttttgatttt ccgctttcga gaaaatgacg gtttagagtt
    21061 acccgaaacc tcaaaaaaac cgaaaccgaa cggactggat tcccgcctgc tcgggaatga
    21121 cggatttcag gattacggtg tatcggggat gatgggaaac ggtggggttg tgtaaaaaat
    21181 gccgtctgaa gcctttcaga cggcatttgc ggcgtttgga cgtttaaaac ttcatttcca
    21241 agctaaatgt gtagtttcgg ccgggggcgg catatcggtt gtaaacgccg acatttttgt
    21301 gttgattgac tgcgccgccg acagtttgcc gcacattttc ccaagtaaca tagcggtagt
    21361 tgaggaggtt gtacacgccc gcacggaggg tgaagtgttt tttaatcgtg taataaccgg
    21421 acacgtccac aatataccaa gggcgggtac ggcgcgcggt ggcttttgta ttgcggctgt
    21481 tgccgttgag caaagcccgg ctgcccaaca actctgtgat ttccttggct ttggaataag
    21541 tcagcatacc gttcacaccc catttgcctt ccggttggtc atagcccaag ccgacgacat
    21601 agcgcgaggg ttggatggca tcaaacaggt gtgattgaat atcggtgcgg tctgcgcgtt
    21661 ttttgatgtc gcggacatgg acacgattat aggcaaatgt agaataccaa ccttcgggca
    21721 atttatccca tacgccgttc caatcgattt tgcccaaaat attgatgccg gtaatccgcg
    21781 cgctttgggc attgaggtaa gccgggtcgc ctttggcttc ttctttgccg tttttaattt
    21841 gcgcttcata accccggaca atcaaatcgc ggtaggcatt attgaaccaa cttgcctcca
    21901 agttgccgaa atcgccttta aacacgatgc cggcttcttt gttgaacgat ttttccggat
    21961 cgattttgac cgccttgctt tgaacacccg cccgccagcc gtacatttcc gcaaacgagg
    22021 gcaggcggaa gccggttgaa gtgcggtaag tcaaatccag ccagtcggca ggtttgagga
    22081 cgatgccggc gttccaggac agggtacggt gcgtgccggt ggaaacgctg ccgtcgtccg
    22141 aatgcgtact gcggtagtcg tagcgcaacc ccgcgccgac atccgcccac ctgcccaaac
    22201 ggacattgtc ccgaactgcc gcgtaatagc ttttgccgtt gatgctgcgc ggcgtacagt
    22261 ccgtataagt attattgccc aagcggcaga tttgctccgt aaccacattt cccccgccta
    22321 tgctgaccca atagggtttt tccttgctgc cgttggggct gatgttttta ttgccgtttt
    22381 tagggggcgt tttcgacgaa taggcgcggt tggcactttg atgataataa tcctgatggc
    22441 ggagattaga gccaaagcgg tcaaacccga gattcacgct caggttgtgg cggattttgg
    22501 cggtatcgaa ggattttttg aatgccgcct gcaagagcct gtggctttcc ccgtaaatca
    22561 cgcggtcgga tttgtagtag gaaaacggct tgtcggcact cggacggcaa tatttgtccg
    22621 aaccgtcggc agaacagtgc gtctgctgaa aatgattgtc caaaccgatg ccctgacggt
    22681 cgtaagagag gcgggcataa tccgcccaag tgtctttatc ggcattggta tagacatatt
    22741 ccaaaccgta gcggcttttg gtgtgcgtct cgtcgtaaaa cacgcccgta ccgtattccg
    22801 cgcccaccag cgcaccgttt tcaccgttgg taaacagacc gccgtatttg tggttgccca
    22861 cgtatttgcc gttaccgggc aaagaacccg cctgttttga atttgcatca aaaaccgcct
    22921 tggtcaggaa tgccggaacc gtcatatcgc gcgtgtcgaa agtttgttgc gtgtgttcga
    22981 gtatgccgcc gatgtagtgc cgtttgtttt caaaacgaaa acccgggcgg aacaaccacg
    23041 accggctttc gtatgaaagc ggatcggcga ggaagcggtt gggacccgtg tagtctcggg
    23101 tagaaaccgt ttgacgttcg tctttgccga caacatcttt tttcggtttg tctttacaag
    23161 ccgcatatcc attagggcat tcttcttcga ccacgaaatg agcgtactga ttggtatcct
    23221 caaccggcac cagcctgtta aagctctgaa cgccgcgtcc tgcatcttcg tgggcgcgga
    23281 tttcccccgc gcgccgcccg gtgtggatca gcaaagcctc cgcaccgccg atgcgccccg
    23341 ccagcgcgat ggattgggta agcccccggt ttttgccgga ataggcggtt ttactctgaa
    23401 tgccccactg cctgccttcc ccgataacat cgtcggcggt tttggtttga aatgcgaccg
    23461 agcccgccaa tgcgccgctg ccttgttcga ccgagtttga gcctttgctg atttcgacag
    23521 ctttgacgtt ttcatactcg atttcattga ttgcgccgct gctgcccgcc gtcctcgtcc
    23581 cgcccaatgc cgcctgcgcg gtgtaggact gtatttgcga aacgccgtcc accgttaagg
    23641 aaacgcggtt tttatccatg ccgcgtattg aatagccgga acttgcgccc cgaccctgtt
    23701 cgaccacggc aatacccgga tcataacggg tcaggtctcg gatattcaaa acctgttctt
    23761 tacttagcgt atcggaagac ttgaccaact tgcccagccc ggttacttcg ttatcgcggc
    23821 gggttttctg ttttttggct tttacctgta tggtatccaa ctgtttttcc tgtgcttgtc
    23881 cggcttgcac attttctgca taagcgggca gcgcagtcat taaagacagg cataaaatat
    23941 tgaatcggaa caaatgttgc tgttgcatag tgtttcccta atcttcgctt tcagacggca
    24001 tcggaaggag cggtgccgtc tgaggcctta ttcttgattg ttcggcagcc gtgcttatcg
    24061 cacaggctgt tggcgtttcg caccgaatac gacagttgca ctgctattgc cggatgcatt
    24121 tgttgcattt ttcgtttgtt tatcgcccgg ataggcaaac catccgccca actcttcggc
    24181 tttgggcccg taaaaaccgc cctgcacctt ggcatctgtg atatatgcct taggcgtgcg
    24241 ggtggtattg ctttgatcga gatcaaaacc tgactcagca gttttcgccg taccttcaaa
    24301 gccgttgtcc ttaatattac catcaatggt aaaggttgcc tcctgcctgt tgtcagcggt
    24361 taacgtacca gtaatttttt tatcggcaaa attcacagta aattccgccc tgttgccact
    24421 cgttgcattg gaagcattgc cgctccagct tgtgcttttg tcgttggcaa tatatccgta
    24481 ccaagacccc cgataaacga tgttttgctc gcttggaatc tctttttcat cggtgcgctc
    24541 gccttggagg aacatacttt gttcaacttg ttccgtttta gcatcagctt gactactgct
    24601 ttctcctgcc tgcatcgcgg acttgctgtt tttgcgcgtc aacattccgt atttcagata
    24661 attgaggttg gaacagcaga cttcgacttc ataggttttt gttttgccat tggtatcacc
    24721 tgccgtattt gaagcggttt gcgccccatt cgtctgcgta cctgcttggg cgtctttttt
    24781 atcactttcc ggcgtgtggt caaatttgcg ggtaaaggct gttccgccat ttgtaccttg
    24841 attggcttga ttgttcccac tttcggaagc ctcgggcaag agcggaatca taatgccgtc
    24901 gacaaccagt tgggcggcgt tgctgaagtt gtcgagcttt tggacttcct tatcgcccaa
    24961 tttcagctcg accgcatcca aaaccgtggt cagcttactg ttttcagacg acgtgcctgc
    25021 cgcaccgttt gatgctgccg catctgtgcc gcctgaagcc accgcgccat tttccgtttt
    25081 gtctttggtt ttcgcgctgc cgacaaccgc aactttttta tcgtcgctca aaaagcggaa
    25141 acccaattcc tcacccttcg gaccgaaaaa gccgccgctc aaagaagacg agtcggaaac
    25201 aaagggatgt agtttggttc ctccagtgtc gggtttgtcg gtcgctatcg ccttaccgtt
    25261 gaagcggttg cctgttattt gggcatcaag gctgtagtat tgggtggtgt atttatcgtt
    25321 agtagtagcg tttgtaactc tattattgcg tattaattta cccgtcaatt ttttactgcc
    25381 gaaatccact tctaaattcg aggtaaaacc ataaccctcg tgcttatcat taaggttgga
    25441 atcagttcta ttggaagttg tttcgccgtc atcgcccgaa aatccgctgt atctgtcgcc
    25501 ttgccctttt gaggtttcaa gaatatcgtt aaatttttgt cccttttcag tatcggttac
    25561 aaaatgccat acgcctttgt agataacttt ttcagaagtg ggaagttgtc gggaagggtc
    25621 tttaccgtga taaaaaatat agccgtcgtc gcctgactta aatttgattt tactgaattc
    25681 tctttcactc tctgcatgtt tataaaacca gccggaataa acatatttga aatttttgta
    25741 atcttttact tcgttttttg gttggtttgc accgctatta gtgctgccgt tttgatggtt
    25801 tgattgcgtg agataagggg aagtgtaaat attgctgcca tcgtctgttt ttactttatc
    25861 aataaccgat tgctgtcgct cgggtaagtt ttggggcttg ctcggcaatc ctgtctcctc
    25921 ccaatcattt gggtttagtt caactttgtc ttcttttgcc tgcggatgcc gattcctccg
    25981 tttcaacctc atcgcaaaac cgtatccgcc ttggtctttt tgggcttgcg gtttttcgga
    26041 agaaacatct tgatattttg gcgcgggacg cggggcttcg gtatcgacag aatcaagatc
    26101 gaaactgccg cctccgccca aacaagcact caacaaaaac acaggcagca ccatagcagc
    26161 ctgatttacc aatggattgt tcatataaac ccaattcaat taaagaatga taaggattat
    26221 tattttattt atttttaaca aatttgcaaa tactttttta tttttttagg gaatacacca
    26281 aaatcccgtc attcccgcgc agtcgtgaat ccgaacgcgt ccgcacggaa acctatatcc
    26341 cgtcattccc acgta
//
LOCUS       DAWKST010000009        25368 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.009, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000009 DAWKST010000000
VERSION     DAWKST010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25368
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.009"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(127..1437)
                     /locus_tag="WJL87_000245"
     CDS             complement(127..1437)
                     /locus_tag="WJL87_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ7798737.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYKQMRRFVAENSSDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLEWLKSLDEEVLKAK"
     gene            1749..2171
                     /locus_tag="WJL87_000246"
     CDS             1749..2171
                     /locus_tag="WJL87_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798738.1"
                     /translation="MEWVSLFSDPQRDDDSLITLKDEKITVKNYIVPWWKKGENFRKL
                     ELITTGKNDNGNLTVNGIYGIQSFADSQLGYTPELLVKSNNDILFKNNLQSVDVKPHS
                     GNIHLDAKGKIQFNLNVPDVDGVQDVASGKKYRQTIVP"
     gene            2257..2754
                     /locus_tag="WJL87_000247"
     CDS             2257..2754
                     /locus_tag="WJL87_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HEZ7798739.1"
                     /translation="MNVKVHKDTHHFGIHNLNNTDVHLTAEDSDNVIEIKGELADLMP
                     TGNVESTSFLRGIETAFKSNTVLTGNNNKISIATEGKGLGLGVNGLQSNSPYSGRTTG
                     IFINNQSSDLTQPSLDGISTIKLKAVKGNNEVDLNVKNHASVKGIIASHSAKATLEAE
                     GDNIV"
     gene            2863..2946
                     /locus_tag="WJL87_000248"
                     /pseudo
     CDS             2863..2946
                     /locus_tag="WJL87_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
     gene            complement(3039..3103)
                     /locus_tag="WJL87_000249"
                     /pseudo
     CDS             complement(3039..3103)
                     /locus_tag="WJL87_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3222..4136)
                     /locus_tag="WJL87_000250"
     CDS             complement(3222..4136)
                     /locus_tag="WJL87_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ7798740.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            4392..5861
                     /locus_tag="WJL87_000251"
     CDS             4392..5861
                     /locus_tag="WJL87_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ7798741.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWASGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            6113..6484
                     /locus_tag="WJL87_000252"
     CDS             6113..6484
                     /locus_tag="WJL87_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798742.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVG
                     SQCRAELDKRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMIGNDVMQMLDPSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            6557..7966
                     /locus_tag="WJL87_000253"
     CDS             6557..7966
                     /locus_tag="WJL87_000253"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ7798743.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGREALD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFKATQSNDEQVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWTEVAAGKGFDLQFALDKVGGKYGFTSSKSTHADRLATIKQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDAAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            8012..8806
                     /locus_tag="WJL87_000254"
     CDS             8012..8806
                     /locus_tag="WJL87_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798744.1"
                     /translation="MTLPMQETRFSILLDELAAKQKAAIAPHLLADGSKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRAARIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKADAWLAGLEAIARVHKKQQFLSQAFARNMMSDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            8994..9770
                     /locus_tag="WJL87_000255"
     CDS             8994..9770
                     /locus_tag="WJL87_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ7798745.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            complement(10438..12909)
                     /locus_tag="WJL87_000256"
     CDS             complement(10438..12909)
                     /locus_tag="WJL87_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEZ7798746.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNLRAGMDTPIAVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHRMPGYPAVQDVREAAVVKLQ
                     AGDVVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGKPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRISDGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMQSEKMPSEQ"
     gene            complement(12906..14084)
                     /gene="hflX"
                     /locus_tag="WJL87_000257"
     CDS             complement(12906..14084)
                     /gene="hflX"
                     /locus_tag="WJL87_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ7798747.1"
                     /translation="MTGRTGGNGSTQAQPERVMLVGVMLDKDGTGSSATRLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLVNLKKQRALRRKPRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIATVRISVAENTGIDALREAIAEYCATAPNTDETEMP
                     "
     gene            complement(14089..15369)
                     /locus_tag="WJL87_000258"
     CDS             complement(14089..15369)
                     /locus_tag="WJL87_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ7798748.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNSRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGSGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            complement(15522..16247)
                     /locus_tag="WJL87_000259"
     CDS             complement(15522..16247)
                     /locus_tag="WJL87_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ7798749.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRISI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(16320..16997)
                     /gene="radC"
                     /locus_tag="WJL87_000260"
     CDS             complement(16320..16997)
                     /gene="radC"
                     /locus_tag="WJL87_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ7798750.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTSQSVRSF
                     RQLGLMP"
     gene            complement(17120..18469)
                     /gene="gshA"
                     /locus_tag="WJL87_000261"
     CDS             complement(17120..18469)
                     /gene="gshA"
                     /locus_tag="WJL87_000261"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ7798751.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNVYALGEILCSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     AAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNSAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            18729..20138
                     /gene="leuC"
                     /locus_tag="WJL87_000262"
     CDS             18729..20138
                     /gene="leuC"
                     /locus_tag="WJL87_000262"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ7798752.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLTPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            20235..20489
                     /locus_tag="WJL87_000263"
     CDS             20235..20489
                     /locus_tag="WJL87_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798753.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            20551..21192
                     /gene="leuD"
                     /locus_tag="WJL87_000264"
     CDS             20551..21192
                     /gene="leuD"
                     /locus_tag="WJL87_000264"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ7798754.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPGGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            21362..21734
                     /gene="dcm"
                     /locus_tag="WJL87_000265"
                     /pseudo
     CDS             21362..21734
                     /gene="dcm"
                     /locus_tag="WJL87_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            21787..22182
                     /locus_tag="WJL87_000266"
     CDS             21787..22182
                     /locus_tag="WJL87_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ7798755.1"
                     /translation="METLEALGYYVSWKVLNAKDFGIPQNRKRIYLTGSLKSKPDLSF
                     ETSPSPKLKNILESGLPTESSPFIKKLLKKFPPSELYGKSVKDKRGGKNNIHSWDIEL
                     KGAVTEEEKQLLNILLKEQRKKNGLQKSA"
     gene            22197..22631
                     /locus_tag="WJL87_000267"
     CDS             22197..22631
                     /locus_tag="WJL87_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ7798756.1"
                     /translation="MPLTKAQISTFYKHPDLQNILDSLTDKGYLVLEHPKQKIGGQRI
                     KDESLPKGYNIVSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGL
                     RLFGYPDDYSFDIPKKDRCDLLGNTVAVPVIKAVSERLLHTL"
     gene            22647..22808
                     /locus_tag="WJL87_000268"
     CDS             22647..22808
                     /locus_tag="WJL87_000268"
                     /inference="COORDINATES: protein motif:HMM:NF021105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ7798757.1"
                     /translation="MIKLTAQQIFDKLLDEEKILSANGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGG"
     gene            22809..23378
                     /locus_tag="WJL87_000269"
     CDS             22809..23378
                     /locus_tag="WJL87_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ7798758.1"
                     /translation="MRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNTGPG
                     FDIADFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWA
                     INLQVKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKK
                     KFEEAYKKHYNRSISIPRWHEIAHKYKKK"
     gene            23412..24482
                     /gene="leuB"
                     /locus_tag="WJL87_000270"
     CDS             23412..24482
                     /gene="leuB"
                     /locus_tag="WJL87_000270"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ7798759.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDAGYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            24645..25208
                     /locus_tag="WJL87_000271"
     CDS             24645..25208
                     /locus_tag="WJL87_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ7798760.1"
                     /translation="MKKIIFAALAAAAVGTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQRPMEKTEVCGGDFSTTIDRT
                     KWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         6221 a         6591 c         6523 g         6033 t
ORIGIN      
        1 ttaatccact ataccacgtt gtctttattg gctttatcaa taaacaggat aagacctgaa
       61 aaaaagccga tacgcctttt tggtgtaccg gctttgccat actgttctgc ttcagacagc
      121 attgcttcat tttgccttta atacttcttc gtccagcgat ttcaaccatt ccagcttttc
      181 gccgattttg atttccaacc cgcgcgggac gggttggtag aagtccggtt cgtccaagcc
      241 gtcgggcata tagctttcgc cggcggcgta ggcgttcggt tcgtcgtggg cgtagcggta
      301 ttcgcgtccg tagcccaatt ccttcattaa cttggtcggg gcgttgcgaa gatggacagg
      361 cacttcgtcg ctgctgtttt ctgcaacaaa tcggcgcatt tgtttatacg ctttgtagcc
      421 cgcgttggat ttcgcggcgg cggcaaggta caataccgct tgcgccaaag ccagttcgcc
      481 ttcgggcgag cctaagcgtt cgaaggtggc ggcggcatcg ttggcgattt ggaaggcgcg
      541 cgggtcggca agcccgatgt cttcccaagc gatacgcacg atgcggcggg cgaggtagcg
      601 cgggtcggtg ccgccgtcga gcatacggca gaaccaatac agcgcggcgt tcggatgcga
      661 accgcgcacg gatttgtgca gggcggagat ttggttgtag aaactctcgc cgcctttgtc
      721 gaaacggcgg atttgcgccc cgagactgtc ggcgagaaat tcggcggtta agtttttcag
      781 acgacgtgta tcggcggcgc gtaaaagttg ttccaacaaa ttcaacaatc tgcgcgcatc
      841 accgtcggcg gtattcacga gtaatttttg cgcatccgtt tcaatcgtaa actcttggta
      901 ttcaggcaaa gccaatacct tggcaatcag ctttttcagg tcgtctgaag acaagggttg
      961 caaaacatac acctgagcgc ggctcaacag cgcgggattg acttcaaacg acggattttc
     1021 cgtcgtcgca ccaataaagg ttagcaaacc gctttcgaca tgcggcaaaa acgcgtcctg
     1081 ctgcgccttg ttgaagcggt ggacttcatc gacaaacaaa atcgtcgcgc gtccctgctg
     1141 caaagcgatt tcggctttat cgattgcctc gcggatgtcc ttcacgccgg aaaatacggc
     1201 ggaaacaggc aaaaactggg cgttgaaact ctgcgccaaa atccgcgcca acgtcgtctt
     1261 gcccacgccc ggcggccccc acagcaacat agaatgcggc ttgccgcctt ctaccgccac
     1321 gcgcaaaggt ttaccttcgc cgatgaggtg ttcctgcccc accacgtcgt caagcgtatg
     1381 cggacgcaat cgttcggcaa gcggcgcgtc gggttctcgg gcaaacaaat cggtcataac
     1441 ggctccgtca acaggttttc aaacaatatg attatacggc agggaacggc ggcgtgccgc
     1501 atacggattc cgcccctccg tttgccttaa gccgatatta ggcgcatact ggaaaagacg
     1561 agagacttca cacaatatat ccggcacgga gaccgattcc gcatcggcat gacaataccc
     1621 aaatcagcgt ttcaattaaa cattaaggag actaaaatag aaaatttgct ttatctacca
     1681 ttgctttgtt gattcaatcg gcattatgtt ttgaggcgga agcccatgaa tatactaata
     1741 ttcaagagat ggaatgggtg tctttatttt ctgatccgca aagagacgat gatagtctta
     1801 taacccttaa agatgaaaaa atcactgtaa aaaactatat tgtgccttgg tggaaaaaag
     1861 gtgaaaactt tagaaaatta gaacttatta ccactggtaa aaatgataat ggaaatttaa
     1921 cggtaaatgg aatttacggc atccaatctt ttgcagactc tcaattggga tatacgcctg
     1981 aattattggt aaaaagcaat aatgatattc tatttaaaaa taatctccaa tctgttgacg
     2041 tgaaacccca ttccggaaat atccatttgg atgccaaagg aaaaattcaa tttaatttga
     2101 atgttcccga tgttgatggg gtacaagatg tagccagtgg aaaaaaatac cgacaaacaa
     2161 tagtgcctta gtgcggtatg tcaacactat cagccccccg atgaaaatga caaaggcagc
     2221 atatcctttc attcaaaaac aggcaatttg ttcaatatga acgttaaagt tcataaagac
     2281 acacaccatt tcggtattca caacctcaac aataccgatg ttcatctgac tgccgaagac
     2341 agcgataatg tgattgagat taaaggggaa ctcgccgacc ttatgccgac aggaaatgtt
     2401 gagagtacaa gttttttaag gggcattgaa acggctttta aatccaatac cgtattaacg
     2461 gggaataaca ataaaatatc catcgccact gagggcaagg gcttgggatt gggcgtaaat
     2521 ggtctccagt ccaactcccc ctattccgga cgcacgacgg gcattttcat caataatcaa
     2581 tcaagcgacc ttacccaacc ttcccttgat ggcatcagca ctatcaagct caaagccgtc
     2641 aagggaaata atgaagttga tttaaatgta aaaaaccacg cctccgtaaa aggcattatc
     2701 gcgtcgcatt ctgcaaaagc aacgcttgaa gccgaagggg acaatattgt ctaggttaaa
     2761 aatccggaca ccaatacggc tttaatcgat gctttgaaaa aacaataccc caatgcttcg
     2821 aaagaactat acagggtggg gggttcaatc cacgactgga acagtacgca tttcgccaaa
     2881 cggcaaaatc gcattaacgg aattgaaaac ttttggaatt gtacaaaacg gcatttgcgt
     2941 cagtttatag tggattaaca aaaaccagta cggcgttgcc tcgccttagc tcaaagagaa
     3001 cgattctcta aggtgctgaa gtaccaagtg aatcggttcc gtactatctg tactgtctgc
     3061 ggcttcgtcg ccttgtcctg atttttgtta atccactata acggcatttc caaagggcat
     3121 tttaagccgt atttgagaga atgcggatgg cgttttcagt gttttcagcg tgataaaatt
     3181 caaatttcta ttttaaaaca atcagcaaag caggatttat ttcagttgtc cgagacaacg
     3241 ggcggctgca ccgcctgcac tccccatact ttttcaatgc actcgagcag gagtgagacg
     3301 tggggattat cctcgtattc ttccgcataa atgaaattga gcggcatact gtggcggcac
     3361 gattcgataa tagccacgcc cgcgccttct tttgccgccg cttcggcttt gtttcccggc
     3421 accattgcca cacctatacc gcctgcaacc aaatcgataa tggtttgggg gtagtcgcac
     3481 aagatctgtt ttttgggtga gagccggttg ctgcgccaaa actggtgcag gtgcttccta
     3541 cttccggaca cgcccgacat ttctatccat acgcattctt gcaggctctg cggaagggag
     3601 cgggtcagat ggggatattg gctttgcggg caaatcagcg cgtaggtcag gttttgcagg
     3661 aatacgctgc ggatgccgcg ttgggcggcg ttgccgagta taaagccgcc gtgcagggtt
     3721 ttgtgttgga tgcgcgagag gatttcgccg ctcattccgt attggatgtg caggcgcgtt
     3781 ttgggggctg tttgaccgat attgtccgtc agcgcgacga gttttgccga atcgatgggg
     3841 tggataatgc ccaaactggt ctcttccgaa taatcgcctg ccagcgtttt ggcaaaatgc
     3901 tccagcttgt gtttgtattg cagcagggat tccgcttcgg gcaacagtat ttcgcccgcc
     3961 cgcgtcaata ccatgccttt ccccgtgcgc ctgaacagcg gcgtgccgac atattcttca
     4021 agggctttaa tttgggcaga aacggcaggc tgggaaagga aaagtcgttt ggcggcttgg
     4081 gtaaggttgc cctcgtgcgc gacggcgaca aatgatttta attgtacggc atccatatat
     4141 ccctccttgt gcggatgttt tctatatttg tgcaatcgaa atcttttagg tggattgttg
     4201 ctgaaaatta actttttaat caagtggttt gtaaattgta tcagttttcc ggatgatggt
     4261 tatcaaaaaa aaagattggt tttattgcct gtttgggctt taaatggggt tacggcttcc
     4321 gaacgcagcc cgtatcaaaa agaaaagtca tgcgcccctt ttacgaggcg cgatatataa
     4381 ggaggaaggt tatggaaaaa cataatggga cttatcggga tttgcaccgt ccagcttcgg
     4441 aatttgcgac gcgggacgaa tatttggaac atgaattgca gattatgcaa ccaaaacgct
     4501 ggcggcccaa cctgcccttt cgcgattacc gcttcgagtg ggaggatttg attcctgcga
     4561 tggcgggaac gattggaaaa gtggtgatgg tgggggcggt ggcggcggcg tttgccgcac
     4621 ctttggggct gcctgacagc tttgtactgg aaaatgtgcg ctatgagctt ttaatcgccg
     4681 ccgcgtttat cttattggta tcgggctttt ttctgcccgg cgccaacctg cccggtacgc
     4741 acgggccgct gattccgatg attcccatcg ttgtgtcggc aggcgggcat cctttggcgt
     4801 tcggcatttc gattgcggtt ttaggtctgc tgatggcttt atttcgcggc ggcagtatta
     4861 tggcgaagct gacaagcaac ggcgtatgcg gcggattatt actctatttg ggctttatcg
     4921 gcacgacggg gcaggtaaaa aaattgtttt cgtgggcaag cggttttaat atgccctaca
     4981 tcgcttttac cgtcattatt gtaacgattg tgatgtacgc tttgttggag cattggaaaa
     5041 aacgctggtt agccgtgcct ttgggatgct tgattgccgg tgtggtggca tttgcattgg
     5101 gtgcgccgtt tgagtttcac accgcccccg gcctgcctcc aatgagtcct gcttattggt
     5161 ggggtgaaaa cagcggctgg catctggggt tgccgacggc agaaagtttt ttggttgtct
     5221 ttccatttgc ggtattggct gttgcaatgt ggtcgcccga ttttttagga catcaagtgt
     5281 tccaaaaaat cagctatccg gaaaaaaccg ataaggtatt gatgaatata gacgacacca
     5341 tgacaagttg ttctgtccgt caagcagtgg gttctatttt agggggtgca aattttacct
     5401 cttcttgggg aacttatatc gtaccggcat cgattgccaa acgccccatt ccgggcggtg
     5461 cggttttaac ggcggtttta tgtattatcg ccgggttatg gggctatccg atggacttgg
     5521 cgatttggca gccggtattg agcgtagcct tggtcgtagg cgtatactta ccgcttttgg
     5581 aagcgggcat ggaaatgacg cgcaaaggca aaaccaccca atccgccgcc atcgtggtgt
     5641 tctcttccgc cttggtcaat ccggttttcg gctgggcgtt gacgatgctg ttggataatt
     5701 tgggcttaat cggctgcaaa gagcgcagtg cgcaattagg tttcgccgga cgcgtgttga
     5761 tacccgcagt aggtttcttg atcttgtgtg tggcgatggg tgcggtcggg atgctgcccg
     5821 gtatcccgcc gtttttggaa cacttcaaat ctttgggcta ggctgaaatc ggaaatgccg
     5881 tctgaaccgc tttcagacgg catttttgca aacaggcaaa atgacggcgg cgggattttt
     5941 tattttcccg attgaagtat aatgttgccg ggcttcaact ggatattcaa aacggtttgt
     6001 tccaacactc ggaacggcgc ataaaacgcc gcccttcgcg ttatcccgaa cggggcggct
     6061 aatcagattc tatcgccata aaaggcgggg tttcaaccga aaaggaattg agatgaataa
     6121 aaccttgtct attttgccgg tggcaatctt actcggcggc tgcgccgccg gaggcggtaa
     6181 cacattcggc agcttagacg gtggcacggg tatgggcggc agcatcgtca aaatggcggt
     6241 cgggagccaa tgccgtgcgg aattggacaa acgcagcgaa tggcgtttga ccgcgctggc
     6301 gatgagtgcc gaaaaacagg cggagtggga aaacaagatt tgcgcttgcg tcgcccaaga
     6361 agcacctgaa cggatgatcg gcaacgatgt gatgcagatg ctggatccgt ccacgcgcaa
     6421 tcaggcactt gccgccctga ccgccaaaac ggtttccgcc tgcttcaaac acctgtaccg
     6481 ctaactttct tcagacggca tgaggcagat gccgtctgaa gccgttttcc tgcaatttcg
     6541 acagacgaga aaaatcatga aatacatcag cacgcgcggc gaaaccgcac acaaaccgtt
     6601 cagcgaagtt ttattgatgg ggcttgcacc cgacggcggc ctgatgctgc cggaacatta
     6661 tccgcaaatc gggcgggaag ccttggacaa atggcgcggt ttggcttatc ccgaattggc
     6721 gtttgaaatt atgcgcctgt tcgttaccga cattccggag gacgatttgc gcgatattct
     6781 gaaccgtact tacacggaag cggcgttcgg tactaaggaa atcacccccg tccgcacgct
     6841 ttcagacggc atcaaaatcc aagccttgtc caacggcccg acgctggcgt tcaaagatat
     6901 ggcgatgcag tttttgggca atgcgtttga atatgtttta aacaaagaag gcaaaaaact
     6961 caatatcttg ggcgcaacca gcggcgatac gggttcggct gcggaatatg ccttgcgcgg
     7021 caaaaaaggt gtgaatgtat ttatgctgtc gcccgatggc aaaatgagcg cgttccaacg
     7081 tgcgcagatg tacagcctgc aagacgggaa tatccacaat atcgccgtga agggtatgtt
     7141 tgacgactgt caggatattg tgaaggcggt gcaaaacgat gccgcgttca aggaaaaata
     7201 ccatatcggt acggtcaatt cgattaactg gggacgcatc gtcgcgcaag tggtttatta
     7261 ctttgccggc tatttcaaag cgacgcaaag caatgacgag caagtcagct tttgcgtgcc
     7321 gagcggcaac ttcggcaacg tttgcgcggg acatattgcc aaacagatgg gcttgcccat
     7381 ccgccgcctg attgtcgcga ccaatgaaaa cgatgtgttg gacgagtttt tcaaaaccgg
     7441 cgcgtaccgc ccgcgcaaca gcgcgcatac ttatgttacc tccagcccat ctatggacat
     7501 ttccaaagcg tccaatttcg agcgtttcgt gttcgacctg atggatcgcg atcctcagga
     7561 aatcaatacg ctgtggacgg aagtcgcggc gggcaagggc tttgacttgc agtttgcctt
     7621 agacaaagtc ggcggcaaat acggctttac ctccagcaag tccacccacg ccgaccgcct
     7681 cgccaccatc aaacaggttt acgagcaaga ccaagaactc atcgacccgc acactgccga
     7741 cggcgtaaaa gtcgcccgcg aagtgcgcga agaaggggaa acggtggttt gtttggaaac
     7801 cgcgttggca gcgaaattcg atgcgaccat acgcgaagcc gtcggcgatg ccgccattcc
     7861 gcgccctgcc gcgctggaag gtttggaaaa cctgccgcag cgcgtgcaaa ccgtgccgaa
     7921 cagtgcggat gcggtaaaag gcatcatcga acaaaccctt gcctgatgtt ccgtacaggg
     7981 gcactccgtc cgaacttaag attgaaagaa aatgacctta cctatgcagg aaacccgttt
     8041 ttccatactg ctagacgaat tggcggcaaa acagaaggca gccatcgccc cccacctgct
     8101 tgccgacggg agcaaggtat ggatacgcaa agcaggcagg cacaatgcgc gatggcgtta
     8161 cgcgctgctc ggtatggttg cccgatattt gaaactgggg gtgttgaagc cggttcccag
     8221 cctcggcggc gagcctgcca ttgcaaccga atcaaaacgc ctgtacgaat tgcgcgcggc
     8281 gaggatagcc gttcccgaat tgctcgccgt caggaaaaat gcccttatgt tcggcaattt
     8341 ggaaggcatc ccgctcgata cgcaaatccg ccaagaagcc gaagccggaa aggcggacgc
     8401 ttggcttgcc ggtttggaag ccattgcacg cgtgcataaa aaacagcagt tcctcagtca
     8461 ggcgtttgcg cggaatatga tgtcggacgg aaaaaacatc agctttctag attttgaaga
     8521 cgacccttcc gaagtcctga ccatagcgca atgccaagcc cgcgattggc tctgctacat
     8581 ccattcgacc gcgctgatat tgaaaaacgg cggactgctg gaagcggctg cggaaaaatg
     8641 gggcggcgta ttgtcggatc agcctgccga aatacaaaag ctgattgccg ccaccgtcaa
     8701 accgatcctc ccgatacgca ggctggaaca cccacgctgg ggacgggacg cactcaggct
     8761 ggcagcctcg atttccctga tttcccttgc cgatatgccg ccataagccg ccgcccgccc
     8821 cgcgatgccg tctgaagccc ttcagacggc atataaccaa cgcgcatcaa gcattgtcgc
     8881 aaccatccgt ttcgtttaga attttggctt tatcccgaat cgggcagaca tttccactct
     8941 gccgcacctc ccgaacaagc actatccatt tcttttactg acaggaaacc caaatggcag
     9001 cattcaatac ccaaaaagta ttgtccgtac accactggac agacgcatat tttaccttta
     9061 cctgcacccg cgacgaatcg ttgcgctttg aaaacggaca gttcgttatg gtcggattga
     9121 tggtggacgg caagccgctg atgcgcgcat acagcgtcgc ctccgccaac tgggaagaac
     9181 acctcgaatt tttcagcatt aaagtccaag acggcccgct gaccagccgc ctgcaacacc
     9241 tcaaagtcgg cgacgacgtg ttaatcagca aaaaaccgac cggaactctg gttgccggcg
     9301 acctgaatcc gggcaaacac ctttacttgt tgagcaccgg taccggcatc gcccctttct
     9361 tgagcatcac caaagacccc gagatttacg agcaatttga aaaaatcatc ctcgtacacg
     9421 gcgtgcgcta caaaaaagat ttggcgtact acgaccgctt taccaaagaa ttgcccgaac
     9481 acgaatacct cggcgacttg gttaaagaaa aactgattta ctacccgatt gtttcccgag
     9541 aagaattcga acaccacggc cgtctgaccg acctgatggt aagcggcaaa ctgtttgaag
     9601 acatcggcct gcccaaaatc aacccgcaag acgaccgcgc gatgctgtgc ggcagccccg
     9661 cgatgctgaa agatacctgc aaagttttgg acgatttcgg tctgaccgtc tctccgaaaa
     9721 ccggcgtgcg cggcgattac ctgattgagc gcgcgtttgt ggatcaataa gctgcttgaa
     9781 aatgccgtct gaacggcatc agcgataaaa aaacccgaat cctgccaatc aggattcggg
     9841 gtttttatag tgggtaaaaa ttagaaatgc acatttccgt cattccaaca caggtgggaa
     9901 tccggactta cctgtttcat tatcgtttga aaataaaaga gaaaccaaac atatagattc
     9961 ccgcttgcgc gggactgacg acggtgttct gcgtagccaa agcagaaaaa catccttatt
    10021 gtcatttcat catgatataa caataaggat gttaaaaatt agttatcatt atatgcatga
    10081 tatataagaa aaattgtccc aataagaaat gttatgcaga aaataaaatg aaaaggatgg
    10141 ttttcattga aaatggttgt tctgccatcg tcatacacag aaccggtatt tggaaaaaaa
    10201 taatccatat tgacatcatt attccattta tcgcataaat ccacttttta atttttttcc
    10261 acattaatat aatctcttat ttaataaaat caaaataaaa atcaaatgcc taacaattcg
    10321 atctcaaatt ttgaacgcca acgtgtttga gcggtaacaa tacatacttt aagtttgcaa
    10381 tccttcatcg tcttattttt gttttcatca actatacgtc taaagcaacg ccgtccgtca
    10441 ttgttcggac ggcatttttt cagactgcat tttcccccgt ttgtgaaggc gcagggcgtt
    10501 gcccaaaacc gccagcgaac tgaagctcat acccagtgcg gctatccacg gttggacata
    10561 gcccaaaacg gcaagcggta cggcaatgat attgtacgcg cccgcccata tcaggttttg
    10621 ccggataata tggcgggtgc gccgcgcctg atcgagcagg tgggcgacgg tacgcaaatc
    10681 ttcgttcaat aacacaatgt ccgcgccgtc cctcgcaata tccgtcccgc ccgctgcggc
    10741 ggcggatacg tctgcctgcg ccaaaacggg cgcgtcgttg atgccgtcgc ctatcatcag
    10801 cacttttttc ccttcttttt gcaaggcttt gacgtattcc agtttgtcct cgggcatggc
    10861 ttgggcgcgg tagtgcgcga cacccagggc acgggcggtt tcggcaacgg cggtttcgcg
    10921 gtcgccgctg aggatgtgca gggtcaggtt tttgcctgcc aactgccgca ccgcctccgc
    10981 cgcgctgtct ttcaaggggt cggtcaggta gaacacggct tggaaaccgc tttgactgcc
    11041 gaggtaaacc gcgctgccgc cgccttctgt ttgcggttct ttacctgaaa tttcggcgac
    11101 ataggatgcc ctgcccaatg cccaaacctg tgtttcgccg ttgacggtca gttgcgcgcc
    11161 cacgccttcg ccgatgcggt tgaggcgttg tttaatagcg atgtcgggga cgctgccgtc
    11221 tgaaatgcgg cagttgagga tggcgcgggc aaggggatgt tcggactgtt gttctaaagc
    11281 ctgcgccacc gcgagaacaa aggcttcgtc tgtgcctctc aacaatgaga tacggcggac
    11341 ggcgggtttg ccttgggtca gcgtgccggt tttgtcgaag atgatgtcgg tggtttgggc
    11401 gagggtttcg attgcctgct ttccgccgat taaaatacct tcgcgcgcca gcgtaccggt
    11461 agaagctgcc agcgcggtcg gcgtggcaag cgataaggcg caggggcagg taatgaccag
    11521 cagggcgacg gtaatccaca atgcggtgtg cgcgtcggcg tacagcgtcc agccgatgaa
    11581 gacggggacg gcaagcagga gttcgccgaa tatgaaagac gaggcgtatt gttccgccaa
    11641 ctcggcagtg cgcggttttt gcgctaaggc gcggtcgagc aggcggacga tgtgcgacag
    11701 tcgcgtgccg ccgccggtgc ggtcggtgcg tataatcagg gggctttgcg tgttgagtgt
    11761 gccggcggtt actttttcag acggcatttt ggcgacgggc aggctctcgc cggtcagcat
    11821 agattcgttg acggcactgc ttccttccag caccgtgccg tcaacgggga tggtttcgcc
    11881 cggtttgacc agtaccacat cgcctgcttg gagcttgacg acggcggctt cgcggacatc
    11941 ctgtacggca ggatagccgg gcatccggtg gcagaaggcg ggtatcagtt tcaccagcct
    12001 ttcggcagca tcgcctgcct tgcgccgggc aatgtgttcc ataaagcgtc cgcccagcag
    12061 gaaaaacagc agcatggcga tggattcgaa atacatcccc tgccccgcat tggtcgcaag
    12121 gctgtaaacg ccggcgataa aggtcatgat gatggcgacg gcaatcggcg tatccatgcc
    12181 ggcgcggagg tttttcaggt cgcgcaacgc gccttgataa aacgggacgg cgcaatagaa
    12241 tacgacgggc agcaccatta aaaagccgcc ccaatggagg atttgcagga aatcgggttc
    12301 gatgtcgccg ccgtaaaggt aggtcggcag cgcgaacatc atcgtctgca tcatccccag
    12361 cccggcaacg gcgaggcgga cgatgtattg ttttcgttct ttttggttgg cggcttcgat
    12421 tttttgcgcg tcatagggtg cggcggtgta gcctatctgc ctgattttca acagaatgtc
    12481 ggaaaggcgg attttgccgt cgtcccagac gacgcggcag cggtgcgtgc tgtaattgag
    12541 gtcgatgcgg acgatgccgt ctgtacgcaa aagctgctgt tcgatcagcc agacgcaggc
    12601 ggcgcaggtg atgccgccga gcattaaaac cgcctcgcgc gtgccgccgt gggtttccac
    12661 aaagtcggac tggacttcgg gcaggtcgta caggcggatt tggtcgagga tttcttgggg
    12721 cggcagctcg gttttttgcg cgtcggcggt gcgttgtttg taataactgc ccaagcccgc
    12781 gtcaataatg ctttgtgcga ccgcctgaca accggcgcag caggtttcgc ggtcttcgtt
    12841 ttcgtaacgg acagtcaggt ggaggtgttc gggaacatcc agaccgcagt ggaaacaggt
    12901 ttttttcatg gcatttcggt ttcgtctgtg tttggtgcgg tggcacaata ctcggcaatg
    12961 gcttcgcgca gtgcgtctat accggtattt tcagcaacgg aaatgcggac ggtggcaatt
    13021 tttcccgcag cgtcgcgcca tatgcccgtg ttttgttctt cagacggcag caggtcggtt
    13081 ttgttgtaca ccttgatgca cggaatatcg ccggcatgga tttcttgcag tacgttttcc
    13141 acgtcttcaa tctgctgtcc gctgttcggg gcggcggcat cgacgacgtg cagcagcaca
    13201 tcggcttgcg cggtttcttc cagcgtggcg gaaaatgccg aaatcagttt gtgcggcaaa
    13261 tcactgacga atccgacggt atcggtcagg ataatgctgc attcgggact gatgtacagc
    13321 cgccgcgccg tggtgtcgag tgtggcgaaa agctggtctt tcgcatatat gcccgacttg
    13381 gtcagccggt tgaacaggct ggatttgccg acattggtat agccgaccag cgcaaacgtt
    13441 ttgattgtgc ccgattcgcg gggcttgcgg cgcagggcgc gctgtttttt gaggttgaca
    13501 agctgttttt tcaaggcatt gatccgatgg gcgatcaatc ggcggtcggt ttccagtttg
    13561 gtttcgccgg ggcctttcat gccgataccg ccgcgctggc tctgcagatg gccgtaaccg
    13621 cgtatcaagc gtcccgccaa atggctcaat tgcgccaact cgacttgcag cctgccttcc
    13681 tgcgtgcggg cgcggcgggc gaaaatcgcc agaatcagcc ctaccctgtc caatacgcgg
    13741 catttcagtt ctttttcaag gttgcgttcc tgcgtgggcg tgagttcgtg gttgaatacg
    13801 accaaatcga tgccgtctgc ggcaactgct tctgacagct ccgccgcctt gcccgtgccg
    13861 acaaacagcg cggtgtgcgg acggtcgcgt ttggcagtct ccacgcgcac ggaatcgccg
    13921 cccgccgctt tgaccagctc gacagcttcc gccaatgccg tctgaaaacc gttcagacgg
    13981 gtggcactgc tgcccgtacc atctttgtcc aacattacgc ccaccagcat gacgcgttcg
    14041 ggttgcgctt gggtactgcc gttgccgcct gttctgcctg tcatgtattc aatcctgtaa
    14101 ataaagggtt acgcctcgtt ccgtttctcc ttccggtgca agcgtgatgc ggctgcccgc
    14161 cattccgtca acgctgaaga cgatgccgcc cgcctcgtgc cttcctatgg acaggcggct
    14221 gccgggtcgc tgccggtagg tataacgggt acacgcttcc aaacaccagc gtccgcgcca
    14281 gtggatgaaa tatttttcgc cttttctaac cgcaagtgtc tcgccgccgt ttacctcaaa
    14341 ataagggtaa tcgggttttc gggttctaaa ctgccatttc gcctgcgcgc gccgtccgtt
    14401 gcggacatag tcgaataccg ccgtataaag cgtgccgtat tccaaaggct tgagcggaaa
    14461 aagcgcgaat tggtacgcgg tcagcctgcc gttggggtcg ttgccggcgg ttaaaaccct
    14521 gacggggcgg atttcgtttt taccctgata cagcttgaaa cttttcatcg taattttgcc
    14581 tgccgcctcg gaaaaatcaa tgctggcagg attgcccgtg atttcatatt ccggcacggg
    14641 gtctggacgc tcgccgtgga aataaggcag tgcgccgctg ccgacgggat aggctgtata
    14701 gagcagctcc tgtgcgggca tggcttcgtc ggtgtacacg accgcaccgt tatggcaggc
    14761 gttgcggtaa tatttccgtc ctgcttccgg ctgatttctg ccttgggcgc aatgccgctc
    14821 aaacctgctg ttgccctgat tgaatacgag aacggtttta ccgttttcgc gcacaaatgc
    14881 cgctcctgcc tcatccgtat ggcggtcaag tagggaaagg cggtggtaga ttgcgctcat
    14941 taatccgtcc acttggcgtt gctgcgtgcg gatgtcgctg tcggacgatt cggcggcttc
    15001 ctcttccgtg ctgatgtttt catgcacgcc gttgtagaga tacccggcaa ggcgtgtgcg
    15061 ttcggtcagc ttttgtgcgg tgtagtgcgg attgtcggga tggtgttcgc cgtgtccgtc
    15121 ttcgggattg agcgtgagat agcgtgcgtg cctgcgggcg gaattttcca aaaccggcgc
    15181 gtgtgccagc ttgtgcaaac cgatttgggc tcggatgcgg ttgaggtagt gcagcgcatc
    15241 gccgccgtcc ctgatttgcg gtgcggacgg atagacaagt tcgcccgcgg gcagggattg
    15301 gttttgggta tgatagaaaa cgcctgccgc cgaacccaac aggagcagcc gaataaaaag
    15361 ggatttcata atgccgtcct cccgacgcgg caacggggtg ttttatcaaa acggcaaact
    15421 tgccaatgcg gtttccgatt gcagattgcc aaaccggcaa acctttcccc gtgccgatgc
    15481 cgaacctgat gccgtctgaa ccttcagacg gcatcggatg tttatttgtc cacccggatg
    15541 ggtgcaggtt catattgtgt cgattcgtcg ccgtaataca gcacgccgag tttgatggag
    15601 attctgccct gcgatttgcg gtgggcattg gaatcgcgca aggaataggc gcaaccgcaa
    15661 tattcctgct ggtagaagtt ttcacgtttg ctgatttcaa tcatgcgcgc gctgccgccg
    15721 cctttgcgcc agttgaaatc ccaatacacc acatcatcgt aaggcgcggc ggcgcggtgt
    15781 ccgcagtcgt tgatttgcgc catatttttc cagcgtgaaa tgcccagcga actggtaaag
    15841 acggggaacc cgtgttcatg cgcgtattgc gccgcctttt caaaacgcat atcgaaacac
    15901 atggtgcagc ggatgccgcg ttccggctca aactccatgc ctttggcttt ggcaaaccat
    15961 tctttgcggt cgttttcgta gtcgtcgtct ttatcgatga aaggaatgcc gaacttttcc
    16021 gcaaagcgca cgttttcctc ttttcggagc atatactctt tgtgcggatg gatattggga
    16081 ttgtaaaaat aaatggtgta gtcgatgccg gaggcaagca tcgcctccat cacttcgccg
    16141 ctgcacgggg cgcagcagga atgcagcaag acttttttat gtccgccggg cgggacgagt
    16201 atggggcggt caatgtcggt aacggtaggt ttgttttgtg tttccattaa cttaacaata
    16261 tctgttgaat acggttgaag cggctatttt atcagagccg ccgcatgtaa aacagagtgt
    16321 cagggcatca acccgagctg tctgaacgaa cggacgcttt gcgaggtaac gataaaatgg
    16381 tcgagcagcg acacatcgac cagcgacatt gcctgtgcca gccgccttgt gaacatgatg
    16441 tcttcctgcg aaggttcggg cgagccgccc ggatggttgt gcgcgataat caggctgtcg
    16501 gcatattcgt ccaatgccag tttgacgatt tcgcggatgt aaatcgtgtt ttccgcaacc
    16561 gtaccgcgcg acagctctct gaccgcaatc agttggtttt ggcggttcag cagcagcgcg
    16621 acgctgactt cgactttttc ctgccccaaa tgaaagcgta aataatcggc caccgtatcc
    16681 ggatcggaca ggacgatgct ctcctgcaat tcttccgcca atatccgccg cccgatttcc
    16741 ctgaccacgg caaactgtgt gaaacttgcc gtccccatcc ctttgtatgc cgacagtttg
    16801 ccgacctccg cgctcatcag cctccccaaa ctgccgaact cctgcagcaa ataacgcgcc
    16861 aaatcaaccg cactcattcc gcgcgtgccg acgcgcagca ggattgccaa aagttcggca
    16921 tcactcaaag ccgccgcccc gcgttccaac agcttttccc tgggcctttc cccctccggc
    16981 cattgcttga tgctcatgtt ttcccttttt taaatagtct gagatgccgt ctgaaacaaa
    17041 tgttcccaca cgccaaatat ccgaaatgcc gtctgaaccg gatttccgct tcagacggca
    17101 tctgtatttc aaaacagtct tacgccgttt cttccaactc gattgaagcc gccagaagcg
    17161 acaggcgtgc cattacgccg taaacgtaga ggcggttgtg ttcgttgtcc acgtcgcagt
    17221 ctttttcaga gcgcggcata cccagcgagt cccattgttc gaatacgcgc ttgcaggctt
    17281 cgggcgcttc ttgggaatta tcgccgttac cggtaggaat gctgttagac agcggaacaa
    17341 acaccatacc gccggcgttg aggttttcgt ccgcaccgcg cccttcgtgt acgcggaaaa
    17401 agccgccgat gacgaagcgg tccatcatat acacgacggg ttcgcacacc gcgctgttta
    17461 aggtttcata agtataaatc ccttcttgga caatcacttc gctgacttcc aaaccttctt
    17521 tgactttcgc cattttattg cggtttttac ggttcaatcc gcgcacttcg tcggcggatt
    17581 tgacgctcat cacgcccatg ccgtaagtgc cggcatcggc tttgacgatg acgaaaggtt
    17641 tgtcggtaat gcccgattcg tcgtatttgg cttgaatttt cgccagcaca cgttctaccg
    17701 cctccgccaa cgcgtcttcg ccttcacgcc cttggaagtc caaaccgccg attttttcaa
    17761 aataagggtt aatttgccat tcgtcgatgc cgattaactt ggcaaattcg gcggcaactt
    17821 ggttgtacgc gccgaaatga tttgttttgc ggcgcgtcgt ccaaccgccg tgcaacggcg
    17881 gcaaaacggt ttggctgatg cctttgagga tgtcgggaat accggcggac aaatcgttgt
    17941 tcaacaaaac cacgcaaggc gaaaagccgt ctgcaagatg gacgcggtcg cgggtacgca
    18001 ataaaggttc caacaggatt ttgtcgccca atgcggtttc aaattcggtc ggttcggtta
    18061 cttccggatt caggctgccc aagcgcactt cataccctgc cgaacacaaa atctcgccga
    18121 gggcgtaaac gttttgcagg taaaacgtat tgcgcgtgtg gttttcggga ataatcaata
    18181 cggatttcgc cgtttcacag gcgcgttgca ccgcatcttg cgcggcaacc gccgccagcg
    18241 ggataaagtt gggattcaga ttattgaagc cgccggggaa caaattcata tcgatagacg
    18301 aaattttgta accggcattg cgtatatcga ccgaaccgta aaacggcggg cggtgtacgc
    18361 tccactgcgt gcggaaccac gcctcgattt tggcgtgatt ggacaggatt tttgcctcaa
    18421 acgcctgaag ttgcgccgaa tgttcgggcg acatcaccgg taatttcatc tttcgtctgc
    18481 ctctgtgtgt tttttgacaa gatgcagatg ataatgcctt tgaaagaatg acgacaagta
    18541 ttctttcgta tcgaatcatg ctttagatat aactgtttac tgaaatggac aatatgtcta
    18601 attttttcgt taaaattgtt tgcaaaataa caccggctca aaaattagac aaaatttgtt
    18661 gcgcggtata aagaatacgt ctaaaatccg cccaatccgc attaccattt atccaaaaga
    18721 acaacatcat gacagcacaa accctctacg acaaactttg gaacagccac gtcgtccgcg
    18781 aagaagaaga cggcaccgtc ctgctctaca tcgaccgcca tttggtgcac gaagttacca
    18841 gccctcaggc atttgaaggc ttgaaaatgg cggggcgcaa gctgtggcgc atcgacagcg
    18901 tcgtctccac cgccgaccac aacaccccga ccggcgattg ggacaaaggc atccaagacc
    18961 cgatttccaa gctgcaagtc gatactttgg acaaaaacat taaagagttt ggcgcactcg
    19021 cctattttcc gtttatggac aaaggtcagg gcatcgtaca cgttatgggc cccgaacaag
    19081 gcgcgaccct gcccggtatg accgtcgtct gcggcgactc gcacacttcc acccacggcg
    19141 cattcggcgc actggcgcac ggcatcggca cttccgaagt cgagcacacg atggcgaccc
    19201 agtgcattac cgcgaaaaaa tccaaatcca tgctgattgc cgttgacggc aaattaaaag
    19261 cgggcgttac cgccaaagac gtggcacttt acatcatcgg gcaaatcggc acggcgggcg
    19321 gtacaggcta cgccgtcgag tttggcggcg aagccatccg cagcctttct atggaaggcc
    19381 gcatgacttt atgcaatatg gcgatcgaag ccggtgcgcg ctcaggcatg gttgccgtcg
    19441 accaaaccac catcgactat gtaaaagata aacccttcgc acccgaaggc gaagcgtggg
    19501 acaaagccgt cgagtactgg cgcacgctgg tgtctgacga aggtgcggta ttcgacaaag
    19561 aataccgttt caacgccgaa gacatcgaac cgcaagtcac ttggggtacc tcgcctgaaa
    19621 tggttttaga catcagcagc aaagtgccga atcctgccga agaaaccgat ccggtcaaac
    19681 gcagcggtat ggaacgggcc cttgaataca tgggcttgga agccggtacg ccattaaacg
    19741 aaatccccgt cgacatcgta ttcatcggct cttgcaccaa cagccgcatc gaagacttgc
    19801 gcgaagccgc cgccatcgcc aaagaccgca aaaaagccgc caacgtacag cgcgtgttaa
    19861 tcgtccccgg ctccggtttg gttaaagaac aagccgaaaa agaaggcttg gacaaaattt
    19921 tcattgaagc cggttttgaa tggcgcgaac cgggctgttc aatgtgtctc gccatgaacg
    19981 ccgaccgcct gaccccgggg caacgctgcg cctccacctc caaccgcaat tttgaaggcc
    20041 gtcaaggcaa cggcggacgt acccacctcg tcagccccgc tatggcagcc gccgccgccg
    20101 ttaccggccg ctttaccgac atccgcatga tggcgtaagt attttcaggc ggtctttaag
    20161 aatttgacgc ggccgtctga aatatgtccc gtaaggcaga aacaccttta atattcaaac
    20221 caaggagttt aaccatgaac aaacttttca ttaccgccct gtccgccctc gccttgtccg
    20281 cctgcgccgg cacttgggag ggcgcgaaac aagacaccgc ccgcaacctt gacaaaacac
    20341 aggccgccgc cgaacgcgcc gccgaacaaa caggcaacgc cgtcgaaaaa ggctgggaca
    20401 aaaccaaaga agccgtcaaa aaaggcggca atgccgtcgg acgcggcatt tcccatctcg
    20461 gcggaaaaat cgaaaacgcc accgaataac cgattcgcga tgccgtctga aaccgcttca
    20521 gacggcatcg tcagcacaag acgcaaaccc atgaaagcct ttaccaaaat taccgccatc
    20581 gtcgccccgc tcgaccgcag caacgtcgat accgacgcca tcatccccaa acaatttctg
    20641 aaatccatca aacgcagcgg tttcggcccc aatgcctttg acgaatggcg ttacctcgac
    20701 cacggcgaac cgggcatgga caacagcaaa cgcccgttga accctgattt ctcgctgaac
    20761 cagccgcgtt accaaggcgc gcaaatcctg ttgacgcgta aaaacttcgg ttgcggctct
    20821 tcacgcgaac acgccccttg ggcattggac gactacggct tccgcgccgt catcgccccc
    20881 agcttcgccg acatcttctt caacaactgc tacaaaaacg gccttttacc tatcgtgttg
    20941 accgaagaac aagtcgatcg gcttttcaaa gaagttgaag ccaacgaagg ctatcagctc
    21001 tccatcgacc ttgccgaaca aaccctgacc acccctggcg gcgaaacatt cacattcgac
    21061 attaccgaac accgcaaaca ctgcctctta aacggcttgg acgaaatcgg actgaccctg
    21121 caacatgccg atgaaattca cgcctttgaa gaaaaacgcc gccaaagcca gccttggctg
    21181 tttaacggtt aaaacctatg ccgtctgaag gccgttcaga cggcatttct gataacatat
    21241 aattgctgca actgatgtaa acagagggat tatcaattgc tttcatcttt caagcgggct
    21301 tagcaacgct ctaaattaat gcaatacaac tctatttcaa ataaattttt agatttttaa
    21361 tatgcaacaa attaaattta ttgacttatt ttccggaatg agtggcatca ggaaaggatt
    21421 cgaacaagcc tgtcggaaac agtcggttgc ttgcgaatgt gtttttactt cagaaatcaa
    21481 accggcagct ttggaagtat tgaagcaaaa ctaccctgat gaagtgccgt atggagacat
    21541 aacgaaaatt gaaacagggg atattcccga ttttgacatt ctgttggcag gcttcccttg
    21601 tcaggctttt tctttcgccg gaaaaagatt gggctttgaa gatacacggg gaacgctttt
    21661 ctttgatgtg gcaaggattt taaaagcaaa aaaaccaaaa ggttttattt tggaaaatgt
    21721 ggaaggattg gtgacacacg atagaaaaga ttcgacacaa aaatagggcg caccctgacc
    21781 gttattttgg aaaccttgga agcattgggc tactatgttt cttggaaagt tttaaatgca
    21841 aaagatttcg gcattcccca aaacaggaag cgcatttatc tgacaggcag tttgaaatcc
    21901 aaacccgact tatctttcga aacaagccca agtccgaaat taaaaaatat tttggaatcg
    21961 ggactgccta ctgaaagcag ccctttcatc aagaaattgc taaaaaaatt tcccccgtcc
    22021 gaactgtacg gaaaatcagt aaaagacaaa cggggaggga aaaacaatat tcacagttgg
    22081 gatattgaat taaaaggcgc agtaaccgaa gaagagaagc aattgttaaa tatccttcta
    22141 aaagaacaga ggaaaaaaaa tgggcttcag aaatcggcat agattggatg gatgggatgc
    22201 ctttgacaaa agcgcaaatt tcaactttct ataaacaccc cgatctccaa aatattttgg
    22261 acagcctgac agataaaggc tatttggttt tagagcatcc gaaacaaaaa attggcgggc
    22321 aaagaatcaa agatgaatcc ctgcccaaag gctacaatat tgtttcaggt aaaaaatctt
    22381 ttgaaatcaa taaaatatta gatccaaacg atgttgcgcc aaccttggtt gcaatggata
    22441 tggagcacct tttcgtcgtt gacaacggcg gtttgcgtac acttaccgga aaagaagggt
    22501 tgcgcttatt cggctatccg gacgattatt cgtttgatat tcccaaaaaa gacagatgcg
    22561 atttattggg taataccgtt gccgtccctg tgattaaggc ggtatctgaa agacttctgc
    22621 atactttata aaccgacagg gataaaatga taaaacttac tgcacaacaa atatttgata
    22681 agttgctgga tgaagaaaaa atcttatcgg ccaatggtca aattagattt ttcttgggcg
    22741 atgtggatat tatcgtcaaa caaaaagatg ttgtcggcaa catcattcag gaatggctcg
    22801 gcggatgatt gagaaaaagg gaaattgaat ttgatgtttc aaccaatacc caaatgcctc
    22861 ccgatttctt tttaaataaa aaagaccgca gcagagaatt gttggaagta aaggcgttca
    22921 accgaaatac cggcccgggt tttgatattg cagattttaa aatgtattct gatgaaatca
    22981 ttcataagcc ctatatgctg gatgtagact atttaatatt cggttacgat atggacgaca
    23041 acggcaatgt aaccatcaag gatttatggc ttaaaaaagt atggcaaatt acgagaagca
    23101 tggatgggtg ggcaattaat cttcaagtca aaaaaggcgt ggtgcataaa atccgcccgg
    23161 gtgtttggta cagcataaat aaaaagaata tgcctatgtt tgaatgcttg gaagattttg
    23221 tttccgcaat tgaagaaacc gtttatcaaa acccggcaac acggcataac gcatccttat
    23281 ggaaaaagaa atttgaggaa gcctataaaa aacactacaa ccgatcgatt tccatacctc
    23341 gttggcacga aattgcacac aaatacaaaa agaaataacc cttcaaccat caacccataa
    23401 ggaaacttga aatgaccaaa catatcgcca tcctccgagg tgacggcatc ggtcccgaaa
    23461 tcgtcgccga aaccgtccgc gtactcgaca aactcatcgc ccaaggtttg gatgccggct
    23521 acgaatacgc cccattgggc ggcgaagcct atgacgaata cggtcatcct tatcccgaat
    23581 ttacgcaaaa cctctgccgc aaagccgatg ccgtcctgct tggtgcagtc ggttctcctc
    23641 aatacgacaa cctcgaccgt ccgttgcgcc ctgagcgcgg cctgttggca atccgtaagg
    23701 atttgaacct gtttgccaat ttgcgtcctg ctgttttata tcccgaatta gccaacgcct
    23761 ccacgctgaa gcctgaaatc gttgccggtt tggatatttt gattgtgcgc gaactcaccg
    23821 gcgacatcta tttcggcgaa ccgcgcggca tccgcgttct ggaaaacggc gaacgcgaag
    23881 gctacaacac catgaaatac agcgaaagcg aaatccgccg catcgcccac gtcgctttcc
    23941 aatccgccca aaaacgcagc aaaaaagtct gctccgtcgg caaagccaac gttttggaaa
    24001 ccaccgaact gtggcgcgaa atttttgaag aaatcggcaa acaataccct gatgtcgagc
    24061 tttcccatat gtatgtggac aatgccgcta tgcagttggt acgcgcgccc aaacaatttg
    24121 acgtgattgc caccggcaac atcttcggcg atattttatc ggacgaggcc tctatgctga
    24181 ccggctccat cggtatgctg ccttctgctt ctttggacga aaacggcaaa ggcctgtacg
    24241 aaccgtctca cggctccgct cccgacatcg ccggacaaaa caaagccaac ccgctggcca
    24301 ccatcctctc gctcgcaatg ttgctgcgtt acagcctgaa cgacgaagcg cgcgcgcaac
    24361 aagttgaaaa cgccgtccaa aaagtgctgc aacaaggctt gcgtaccagc gacatttacg
    24421 aagaaggcac aaaactcgtt tcctgctccg aaatgggcga tgcggtactc gccgccttgt
    24481 aaaaaaacaa agcgcggatc acaccgcgct ttttcatctg cccttttccg ctcgccccgt
    24541 tcgtcaaccg cctgcaaagc caccgtttac cggcaggaaa tgccataaat ccccgcctat
    24601 gttaggataa acgcctttac tggctcacac aaaggattat catcatgaaa aaaatcatct
    24661 tcgccgcact cgcagcggca gccgttggca ctgcctccgc cgccacctac aaagtggacg
    24721 aatatcacgc caacgcccgt ttcgccatcg accatttcaa caccagcacc aacgtcggcg
    24781 gtttttacgg tctgaccggt tccgtcgagt tcgaccaagc aaaacgcgac ggtaaaatcg
    24841 acatcaccat ccccgttgcc aacctgcaaa gcggttcgca acactttacc gaccacctga
    24901 aatcagccga catcttcgat gccgcccaat atccggacat ccgctttgtt tccaccaaat
    24961 tcaacttcaa cggcaaaaaa ctggtttccg ttgacggcaa cctgaccatg cacggcaaaa
    25021 ccgcccccgt caaactcaaa gccgaaaaat tcaactgcta ccaacgcccg atggagaaaa
    25081 ccgaagtttg cggcggcgac ttcagcacca ccatcgaccg caccaaatgg ggcgtggact
    25141 acctcgttaa cgttggtatg accaaaagcg tccgcatcga catccaaatc gaggcagcca
    25201 aacaataaat cttgatgcga atcagcacaa atgccgtctg aacgtccttc aaacggcatt
    25261 tgtgccgtct tcatcaaacc gccccgccaa cggtaagccg atatggcgga gcacaatccg
    25321 ccgaaagata tttcagcggc aggcagggct tgccggaaag tccggaaa
//
LOCUS       DAWKST010000010        22450 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.010, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000010 DAWKST010000000
VERSION     DAWKST010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22450
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.010"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            43..606
                     /locus_tag="WJL87_000272"
     CDS             43..606
                     /locus_tag="WJL87_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ7798761.1"
                     /translation="MRHLENNGYANVAGLERILAVKTDNYKEKENLLHEIFSKSRIGD
                     TELFAVDENLVKRLFLSLRGEIVFPKNETAESEFEKSVHERRQEGNAGSGRKQLLDLV
                     RRGHREYPYALPRLLAGAASYKPKKSKIRLFKEAYFGKSGTRLTDEIADGIHIYTCFS
                     RADLEKAYSEYLELFKSESDAEGRKPQ"
     gene            643..1404
                     /locus_tag="WJL87_000273"
     CDS             643..1404
                     /locus_tag="WJL87_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEZ7798762.1"
                     /translation="MMGDSVIYYVEQADKPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNVEQGESVCV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLAGWQNVADLLEPRH"
     gene            1459..1800
                     /gene="fdx"
                     /locus_tag="WJL87_000274"
     CDS             1459..1800
                     /gene="fdx"
                     /locus_tag="WJL87_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEZ7798763.1"
                     /translation="MPKITVLPHTTLCPEGAVIEDAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(2087..2674)
                     /locus_tag="WJL87_000275"
     CDS             complement(2087..2674)
                     /locus_tag="WJL87_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ7798764.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHYRSRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLAGSGGGFT
                     VTAESVSAACPELEGRYLELVRRTATSFGFVQVQEYPAGGQEETQAVPDAASDHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            2887..3084
                     /gene="iscX"
                     /locus_tag="WJL87_000276"
     CDS             2887..3084
                     /gene="iscX"
                     /locus_tag="WJL87_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ7798765.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(3152..3553)
                     /locus_tag="WJL87_000277"
     CDS             complement(3152..3553)
                     /locus_tag="WJL87_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ7798766.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTATLREERKQL
                     DQFSRITSAYPDGRPTKRDRRQLDRLKKGDW"
     gene            3764..4723
                     /locus_tag="WJL87_000278"
     CDS             3764..4723
                     /locus_tag="WJL87_000278"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ7798767.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDYAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQRLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            4791..6101
                     /gene="tilS"
                     /locus_tag="WJL87_000279"
     CDS             4791..6101
                     /gene="tilS"
                     /locus_tag="WJL87_000279"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ7798768.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKRAGFVPEALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAAFAEKGFDILALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNTDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLDEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFRQEKTDKLRFLKDRQISGNLR
                     EILTGQGFVLKRHPFGLPEHLLEQDGILRTVAVSDTLAVGGIHKDVKKILQGKRVLPV
                     LRPIWPLVADSGNRPLALANCCADFQYSVSDGILPVHPDFPILF"
     gene            6175..6960
                     /locus_tag="WJL87_000280"
     CDS             6175..6960
                     /locus_tag="WJL87_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ7798769.1"
                     /translation="MRHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGMPVGVYIPEGKMPSEEIRRLRAVVPEGRIFSVSDGILKKISSLSCADDILALIDI
                     PLGGTLPDKGDCVVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGCADAWSPKVLRAGM
                     GAHFLSGIYPQADLEIWLARYKGRVFATALREEKQAVLYGEDLCGPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRSAY"
     gene            6970..7167
                     /locus_tag="WJL87_000281"
     CDS             6970..7167
                     /locus_tag="WJL87_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798770.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLAWVWWKWF"
     gene            7429..7782
                     /locus_tag="WJL87_000282"
     CDS             7429..7782
                     /locus_tag="WJL87_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798771.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEER"
     gene            7794..8099
                     /locus_tag="WJL87_000283"
     CDS             7794..8099
                     /locus_tag="WJL87_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798772.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDLVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPRENTHKDASNTGAKTH"
     gene            8161..9537
                     /gene="mpl"
                     /locus_tag="WJL87_000284"
     CDS             8161..9537
                     /gene="mpl"
                     /locus_tag="WJL87_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ7798773.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIGVYEGFDTAQLDEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFGVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHYL
                     VRTVPSEGLIVCNGRQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     TAGRVKWDLMGRHNRMNALAVIAAARHAGVGIQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRIGGARILAVLEPRSNTMKLGTMKSALPVSLKEADQ
                     VFCYAGGVDWNVAEALAPLGGRLHVGKDFDAFVAEIVKNAEAGDHILVMSNGGFGGIH
                     GKLLDALR"
     gene            9744..10796
                     /gene="bioB"
                     /locus_tag="WJL87_000285"
     CDS             9744..10796
                     /gene="bioB"
                     /locus_tag="WJL87_000285"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ7798774.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLELVHQAA
                     EVHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFELE
                     GEVAEVEKASGIKVDY"
     gene            complement(10875..11508)
                     /locus_tag="WJL87_000286"
                     /pseudo
     CDS             complement(10875..11508)
                     /locus_tag="WJL87_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            complement(11632..11871)
                     /locus_tag="WJL87_000287"
     CDS             complement(11632..11871)
                     /locus_tag="WJL87_000287"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798775.1"
                     /translation="MGGCTFFRRTAFVKQRAFEVADGNVRVLQGRFDVFQNIGIGQVI
                     DRIIAAEHNVAHADNGNGIGCLFVRRILSVHIAGI"
     gene            11831..12091
                     /locus_tag="WJL87_000288"
     CDS             11831..12091
                     /locus_tag="WJL87_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ7798776.1"
                     /translation="MTKAVRRKNVQPPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(12154..14013)
                     /gene="ilvD"
                     /locus_tag="WJL87_000289"
     CDS             complement(12154..14013)
                     /gene="ilvD"
                     /locus_tag="WJL87_000289"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ7798777.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHVGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDITNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(14186..15955)
                     /gene="cysI"
                     /locus_tag="WJL87_000290"
     CDS             complement(14186..15955)
                     /gene="cysI"
                     /locus_tag="WJL87_000290"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ7798778.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAVSDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPIRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVAEADKAKIEELARTYGLIRNDVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILSELDDLIGKWAAGRDTDEGFGDFAIRTGI
                     VKPVLDAPVDFWDASKAVPIARA"
     gene            complement(15982..17796)
                     /locus_tag="WJL87_000291"
     CDS             complement(15982..17796)
                     /locus_tag="WJL87_000291"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEZ7798779.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPAAEPFSVTVLSASQTGNAKSVADKASDSLEAAGIQVRRAELKD
                     YKAKNIADERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCRAGKDFDRRFEELGAKRLLERVDADLDFAAAADGWTGRIVARLKEEAAKNRATP
                     APQTTPPAGLQTAPDGRYCKADPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDPATEIQAGGKTLPVASALLSHFELTQNTPAFV
                     KGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARAGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFARDFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(17793..19079)
                     /locus_tag="WJL87_000292"
     CDS             complement(17793..19079)
                     /locus_tag="WJL87_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ7798780.1"
                     /translation="MTAQHQAPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKETIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLNCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDVHFVPVSALLGDNLVYPGGNMPWYRGEPLLVIL
                     ENLPGADETGRSAADFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(19118..20041)
                     /gene="cysD"
                     /locus_tag="WJL87_000293"
     CDS             complement(19118..20041)
                     /gene="cysD"
                     /locus_tag="WJL87_000293"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEZ7798781.1"
                     /translation="MTKTEPNNVQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPAEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(20079..20813)
                     /locus_tag="WJL87_000294"
     CDS             complement(20079..20813)
                     /locus_tag="WJL87_000294"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ7798782.1"
                     /translation="MKIFKPALWKIPPIENGSETALAEKTETLKQRLHRIAGSHRDAR
                     FASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHAETLNLLDRIERVYPHMQIKRFQP
                     IREDALHYVESKGRFAFYDSVEARRECCRIRKTEPLDRAIAGADAWLTGQRREQSATR
                     TELPFAEYDAGRGIDKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCTRP
                     VKAGEDIRAGRWWWEDKNSKECGLHK"
     gene            complement(20829..22280)
                     /gene="cobA"
                     /locus_tag="WJL87_000295"
     CDS             complement(20829..22280)
                     /gene="cobA"
                     /locus_tag="WJL87_000295"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ7798783.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKGKLKSVTERRRFWEKQFNGRFAALVKNRQNTLAERELAKQLEQNYQGGFVSL
                     VGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGGRTP
                     QEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAAVGA
                     TAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALIAER
                     LQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLHEKL
                     AWFGENAKKESNPAEHAYFALDGLGTGQEQQAA"
BASE COUNT         4848 a         5831 c         6460 g         5311 t
ORIGIN      
        1 tataaaatcg gcatttcgga tgtaagcaat tttgaaggca gaatgcgcca tttggaaaac
       61 aacggctatg cgaacgttgc cggattggaa cgcatcctcg ccgtcaaaac cgacaattac
      121 aaagaaaaag aaaacctgct ccatgaaatt ttcagcaaaa gcaggatagg cgataccgaa
      181 ttgttcgccg tggacgaaaa ccttgtaaaa cgtttgtttt tatcgcttcg cggcgaaatc
      241 gtgtttccga aaaacgaaac ggcggaatcg gaatttgaaa aaagcgtcca cgaacgcagg
      301 caggaaggga atgccgggtc cggccgcaaa caactgcttg atttggtacg gcgcggacac
      361 cgggaatacc cttacgcgct gccccggctt ttggcgggcg cggcatccta caagccgaaa
      421 aaatcgaaaa tccgcctttt taaagaagca tatttcggca aaagcggcac gaggctgacc
      481 gacgaaattg cagacggcat ccatatttac acctgttttt cgcgggcgga tttggaaaaa
      541 gcctattccg aatatttgga acttttcaaa tccgaatcgg atgccgaagg cagaaagccg
      601 cagtaaggtg caaacagata ccgtacacgt tgaggagcag atatgatggg cgattccgtc
      661 atttattatg tagaacaggc agacaaaccg gtaaaccgtg ccggcgaacg cgcccgcaaa
      721 acattcaaat atttttggcg cgagcttttt tgggaacgcc gccgcatcat tcccgcctta
      781 gattttgcca tggtcaaagc cccttttttc caagacggcg aagacggcga aatttgcgaa
      841 catatgtgga tcgacgatat ttattttgac ggtctttaca tttatggcgt gctgaacaat
      901 gaacccggcg aactgaccaa tgtcgaacaa ggcgaaagcg tttgcgttcc ggttgacgac
      961 atcagcgact ggatgttcgt gtgcaacggc attccctacg gcggctttac catacaggca
     1021 atgcgtggac agatgacgga agaggagcgt accgaacacg atgccgcatg gggaatcgat
     1081 ttcggcgatc ccgggcaggt attgctggtg tatgaagaaa aagaacatcc cgaaaatctg
     1141 gaagagcatc cgatgtgccg gaactgtatt gacgattttc ggcaacagtt gtcccaaaat
     1201 ccggattttc tgcatgagca ggacgaagac ggctatacgc cgcttcatca tgaagccatc
     1261 gcaggaaatg cacttatggt tcaagccatg cttgaatacg gcgcaaatcc tgcctcaaag
     1321 acatcggaag gctataccgc cctcgatttt gcccgcctgg cgggctggca aaatgttgcc
     1381 gacctgctcg aaccgcgaca ttaggcggac agttttccga aaacgaacac aaacactttt
     1441 tacagaaaga caataaaaat gcccaaaatc accgtacttc cacacacgac attatgcccc
     1501 gaaggcgcgg ttatcgaaga cgcgcccgaa ggcaaaaccg tccttgacgt gctgctcgac
     1561 catgatatcg aagtcgatca cgcctgcgaa aaatcctgcg cctgcaccac ctgccacgtc
     1621 atcatccgca aaggtttcga cagtttggaa gaaccgaccg aattggaaga agacctgctc
     1681 gaccaggctt ggggtttgga agccgattca cgcctgagtt gtcaggcggt tgtcgccggc
     1741 gaggatttga ttgtggaaat ccccaaatac accatcaacc acgcgcgcga agaacactga
     1801 aaacaggccg tctgaagccg gcacgcttca gacggcattg ttgcgcggat aaggcgcaat
     1861 cgcccgaaaa caggcgttcg tacaggcgga actttcgatt ctatagtgaa ttaacaaaaa
     1921 tcaggacaag gcggcgagcc gcagacagta ccaatagtac ggcaaggcga ggcaacgctg
     1981 taccggttta aatttaattc actatatatt gatttttatc ggttttctga cggaacggat
     2041 tggtgcggca tccgaggcgg attactcggg cgcgatgcac gggtatttat cggttttgca
     2101 gccggaaaaa ccgccggcgg gttactttca gggcggcggt cgggcggtat gccggatagc
     2161 gttccacttt cgcttctata ttgattttga ttggttttct gacggaatga tccgatgcgg
     2221 catccgggac ggcttgtgtt tcttcctgcc cgcctgccgg atattcctgt acttgcacga
     2281 aaccgaaaga ggtggcggtg cggcggacaa gctcgagata gcgtccttca agctccggac
     2341 aggcggcgga cacgctttct gccgtaaccg tgaagccgcc gcccgacccg gcaaggcgtt
     2401 ccgcaatctg cgtgtagatg cgccaacgtt gggcgcgcaa cattgccgca tcgcaaccgg
     2461 cagccgcttt atccaaggca atcagaccgc gcctgctccg gtagtgaagg caaaccgtta
     2521 ctctggagag gatatgttgc ccgtccaata tttgatacag tttgcgtatc agcagaatca
     2581 ggcggtcaaa ctcctccatc tccgacaggg aatcaggatg gtcggaagcg gtataaacca
     2641 gtttggacaa ttttgcggca aacatattgt tcatcaatct tccttgtcgg ttgaaacccc
     2701 gctcttcggg gcggtagaat cagatttgtt tgggagggac aactcctccc agatcaggac
     2761 gacacatagg ctggtgcttg atgtgttgtc cggcgagttg aaacattcag ccatcctcaa
     2821 ggtgcggcag ttttgccgaa acatattcta cacggcttca atgccggacg ataaaaggaa
     2881 attcatatga aatggaccga cacccggcgt atcgccgaag aactctatga cctgcacggc
     2941 gaaaccatcg atcccagaac cgtgcgcttt acccaactgc gcgacctgat tatggcattg
     3001 cccgaatttg acgacgaccc cgcccgttgc ggcgaacgca tcctcgaagc cgtgcagcag
     3061 gcatggatag acgaggcgga ataagtttcg ggaatgccgt ctgaaatgcg gcggtacgcg
     3121 gttcgtgctt ctgtttgcag cgggaatggt tttaccagtc tccttttttc agcctgtcca
     3181 gttggcggcg gtcgcgcttg gtcggtctgc cgtcgggata ggcggaagtg atgcggctga
     3241 attggtcgag ctgtttgcgc tcttccctca atgttgccgt tttcgcgtcc tcttcataca
     3301 gaagccgcgc ctcggatgcc gggcggcgtt ggtggttcaa acctttaacc ttgattttat
     3361 agggaaggga attgagcgtc aggtcgataa tatcgccgat gtctatggtt ttactgtttt
     3421 tgaccttcga gccgtttact tgaaccctac ccagttcgat gtgcttttgc gcaagggaac
     3481 gggtcttgaa aaaacgtgcc gcccaaagcc atttgtccag ccgcatggcg gaagaatcgt
     3541 gcttgtcttt catacgattt tgtttgaaat aattgaattt gtttcaagtt tagcataaga
     3601 tacgccgcct ataactagta tatatgcact aatccactgt tttccatgct gtccgaacac
     3661 aaaaagaggg tatggaaaag ccgttttgga caataaatta actgcggaat atgcacaaat
     3721 agcgtatgat agcggcagaa tctgttgatg agagcttcat tctatgaaac ctgttttttt
     3781 ggattttgaa caacccatag ccgaactgac caacaaaatc gatgagctgc gtttcgtcca
     3841 agacgagtct gccgtcgata tttcggacga aatacaccgt ttgcagaaaa aaagcaacga
     3901 tttgaccaag tctattttca gtaaacttac acccgcccaa atttcacagg tttcccggca
     3961 tccgcagcgt ccctatactt tggattacat tgaggcactg tttaccgatt ttgaagaact
     4021 gcacggcgac cgccactttg ccgacgatta tgcgattgtc ggcggattgg cgcgtttcaa
     4081 cggacaaagc gtgatggtcg tcgggcatca gaaagggcgc gacaccaaag aaaaaatccg
     4141 ccgcaacttc ggtatgcccc gtcctgaagg ctaccgcaaa gccctgcgcc tgatgaagac
     4201 ggcagaaaaa ttcggcttgc ccgtcatgac ctttatcgat acgccgggcg cgtatcccgg
     4261 catcggcgcg gaagaacgcg ggcagtcgga agccatcggc aaaaacctgt acgaactgac
     4321 acgcctgcgc gttcctgttt tgtgtaccgt catcggcgaa ggcggttcgg gcggtgcgtt
     4381 ggcggtcgcc gtaggcgatt acgtcaatat gctgcaatat tcgacctatt ccgttatttc
     4441 ccccgaaggc tgcgcgtcta ttttgtggaa aacggcggaa aaggcggcgg atgcggctca
     4501 ggctttgggc attaccgctg accggctgca aaggctggac ttggtcgata ccgtcatcaa
     4561 agaaccattg ggcggtgcgc atcgggattt cgggcaaacc atgaaaaacg taaaagccgt
     4621 tttggaaaaa caactgcacg aagcgcaaag catcccgctt gccgatttgc tttcgcgccg
     4681 tttcgaccgc attatggctt acggcaaatt ttcggaacaa taattcaggt agaacaagca
     4741 gcaagcagtt tgtctgaaac tgcttgcttt ttctttatcg ggacggaacc gtgctgactt
     4801 tagatgcgtt tgagcaatgc ttgaaggatt gttttcctca aggtctgaat ggaaaaaaaa
     4861 cagcggtggc attaagcggc ggcttggatt ccgtggtctt gctgcacctg cttgtccgcg
     4921 ctgggaaacg ggcaggattt gtgccggagg cattgcatat ccatcacggc ttgagccccc
     4981 gtgccgacga ttgggcggat ttctgtcaaa actattgcga tatgctaggg gtggggctgg
     5041 aaacggttaa ggtctgcgtg gaaaaaaacg gtttgggcat cgaagcggcg gcgcggcaaa
     5101 agcgttatgc cgcgtttgcc gaaaaaggct ttgacatttt ggcgttggcg caccacaggg
     5161 acgatcaaat cgaaaccttt atgctggcgg tcgcgcgcgg cggcggtttg cgcgctttgg
     5221 cggctatgcc cgccgtccgc ccttttgggg aaaaaggcat catttggcgg cccttgctgc
     5281 ctttttcgcg ccaagatata tgggattatg cccaaaaaca cggtttgccg aatatcgagg
     5341 atgaaagcaa taccgatacg gcttatttgc gaaaccgctt ccggcaccgt attttgcccg
     5401 aactttcggc gcagattccc catttcgggc gacatgtgct gaacaatgtc cgcgctttgc
     5461 aggaagattt ggctttgttg gacgaggtcg tcgttcagga ctgccgttgg gtttgcgggg
     5521 ccggttattt cgatacggcg cggtggctga cgttttcccc gcgccggaaa acccatattt
     5581 tgcggcattt tctgaaggaa aacggcattc ccgtgccgaa tcagaatgcc cttgccgaca
     5641 ttgcccgggt tttgacggag gcaaaaaccg gacgttggaa cttgcaaggc tttgaattgc
     5701 atcattatgc aggcaggctg tttgtgttcc gacaggaaaa aacggataaa ctgcggtttt
     5761 tgaaagacag gcagataagc ggaaatttaa gggaaatatt gacggggcag ggatttgttt
     5821 taaaacgtca tccgtttggg cttcctgagc atcttttgga gcaggacgga attttgagga
     5881 cggtagcggt atcggatacg ttggctgtcg gcggcatcca taaggatgtg aaaaaaatcc
     5941 ttcaggggaa acgggttttg cctgtcctgc gcccaatttg gccgcttgtt gccgacagcg
     6001 gaaaccgtcc attggcgttg gcaaactgtt gtgcggattt ccaatactcg gtttcagacg
     6061 gcattttgcc cgtccatcct gactttccca ttttattttg ataatatcgc aaacagattt
     6121 cggcggcgtt cagtcgggta ttgtccggtt gcatatttct aaaaggcttg tgaagtgaga
     6181 cacatcagtt cgaccaataa tgaacacatc cggcacctgc accgcctgtt gtcgcaagga
     6241 aagttcagac ggcaatacgc ccaaaccgtt ttggagggcg tgcacctgct tcaggttttc
     6301 ctgcaatccg gcgggatgcc ggtcggggta tatattcccg agggcaaaat gccgtctgaa
     6361 gaaatccgca ggctgagggc ggttgtgccg gaaggcagga ttttttccgt ttcagacggc
     6421 atattgaaaa aaatcagtag cttgagttgt gcagacgata tacttgcact gattgatatc
     6481 ccattgggcg gaaccttgcc ggacaaaggc gactgtgtgg ttttggacgg cgtgcaagat
     6541 ccgggcaatg tcggcaccgt cttacggagt gcggcggcgg caggggtagg tacggtcgtt
     6601 ttgggcaggg gttgcgcgga cgcgtggtcg cccaaggtat tgcgcgcggg gatgggcgcg
     6661 catttcttgt cgggcattta tccgcaggcg gatttggaaa tatggttggc gcgctataaa
     6721 ggccgtgtgt ttgccaccgc cttgcgcgag gaaaagcagg cggttttgta cggcgaagat
     6781 ttgtgcggac cgacagcctg ggtgtttggc aacgaaggcg cgggggtcgg taaagcggtt
     6841 ttggacaggg cggacaagtg tgtcaggatt ccgatgcacg atgcaaccga gtctttaaat
     6901 gtcgcgatgg cggcgacaat ctgcctgttt gaacagatgc ggcagcggtc ggcgtattga
     6961 ggaaaagaaa tgccgtctga aaaaatctat tacggcgtat tgattttctt atgtatcgct
     7021 tctatgctgc tgtcgccgtt tttttatgcg ggtgctttga agcccaagaa ggcggcattg
     7081 cggaaggacg ggcagtggaa actcatctta ttgtccaata ccgtggcggc ggcggttttg
     7141 gcttgggtgt ggtggaaatg gttttgacag atattgctca aaatcgtgct aatggaatcc
     7201 gaacaaataa agaatttggt aaaaaatttg ttaaatcaac gaattaaagt tttgtggaaa
     7261 acaaaacagc tctaagcaaa tagggcgttt gtcggtaaat acggaagagt tgcggcatta
     7321 tcgggcatct ttaacaagta gtgccgtctt ggcaggcaat tggtttttat gggcagcttg
     7381 caaaatcgcg gatataaaat tgcgaatcgg ttaaagtgtg gggacgctat gaaaaattgc
     7441 gaattttttt atgacccgac aagggcaatc tatgatagcg gtgcagatta cttaactagg
     7501 gaaaaacata gattagtcgt aattgcaaat agtgcttggg ggctattgct taatttatct
     7561 tgttattatg acgaggtttt ggaaaagcgg aaaataccgt tcggcaaaca ggaaattgat
     7621 gacgatatgg acaaagtgtc cgcccttaag cggaagttta aagatatttc tgaaatcaaa
     7681 gtaggggatg gttgggaata cccgttcaat tatgagcagg gaatgaaaga attagatgaa
     7741 gtgctattga aatacattcc cttttttgaa gaagaacgat aaaggaggtt gatatgcgcg
     7801 tatctaaaat aattggaagt atgttgcttg ttacagcggt tcagaccgta ttttcggcaa
     7861 atgtttacgc gtgccgccat aatggtaaaa ccagttacag ccaaactccg ggaaaacatt
     7921 gtaccaacgc gggtttgggg cgggacctgg tgtacagttc ggttagaccg gcagtaaaag
     7981 acagggcgga agacgcaggg gtcggcgatt attcggacac ggtgagggac gaacacgtcc
     8041 aaaatccgag agagaatacg cataaggatg cttcgaatac cggtgcaaag acgcattgat
     8101 tgaaaccgaa tcagccatga cggcgttgaa taatagaatt tgaacgatta gggaactctg
     8161 atgaaacaca tccacattat cggtatcggc ggcacgttta tgggtgggat tgccgccatt
     8221 gccaaagaag cagggtttga agtcagcggt tgcgatgcga agatgtatcc gccgatgagc
     8281 acccagctcg aagccttggg cataggcgtg tatgaaggct tcgacaccgc gcagttggac
     8341 gaatttaaag ccgacgttta cgttatcggc aatgtcgcca agcgcgggat ggatgtggtt
     8401 gaagcgattt tgaaccgtgg gctgccttat atttccggcc cgcaatggct ggctgaaaac
     8461 gtgctgcacc atcattgggt actcggcgtg gcggggacgc acggcaaaac gaccaccgcg
     8521 tctatgctcg cgtgggtttt ggaatatgcc ggactcgcac cgggcttcct tatcggcggc
     8581 gtaccggaaa atttcggcgt ttccgcccgc ctgccgcaaa cgccgcgcca agacccgaac
     8641 agccaatcgc cgtttttcgt tatcgaagcc gacgaatacg acaccgcgtt tttcgacaaa
     8701 cgctccaaat tcgtgcatta ccgtccgcgt accgccgtgt tgaacaatct ggaattcgac
     8761 cacgccgaca tctttgccga cttgggcgcg atacagaccc agttccacta cctcgtgcgt
     8821 accgtgccgt ctgaaggctt aatcgtctgc aacggacggc agcaaagcct gcaagatact
     8881 ttggacaaag gctgctggac gccggtggaa aaattcggca cggaacacgg ctggcaggcc
     8941 ggcgaagcca atgccgacgg ctcgttcgac gtgttgctcg acggcaaaac ggccggacgc
     9001 gtcaaatggg atttgatggg caggcacaac cgcatgaacg cgctcgccgt catcgccgcc
     9061 gcgcgtcatg ccggtgtcgg cattcagacg gcctgcgaag ccttgggcgc gtttaaaaac
     9121 gtcaaacgcc gcatggaaat caaaggcacg gcaaacggca tcaccgttta cgacgacttc
     9181 gcccaccacc cgaccgccat cgaaaccacg attcaaggtt tgcgccaacg tatcggcggc
     9241 gcgcgtatcc tcgccgtcct cgagccgcgc tccaacacca tgaaactcgg cacgatgaag
     9301 tccgccctgc ctgtaagcct caaagaagcc gaccaagtgt tctgctacgc cggtggcgtg
     9361 gactggaacg tcgccgaagc cctcgcgcct ttgggcggca ggctgcacgt cggcaaagac
     9421 ttcgatgcct tcgttgccga aatcgtgaaa aacgccgaag caggcgacca tattttggtg
     9481 atgagcaacg gcggtttcgg cggaatacac ggaaagctgc tggacgcttt gagatagccc
     9541 gggcgatgcc gtctgaaagc ccttcagacg gcatcgtccg gctgcgcggc acaaaggcgg
     9601 aaaaaccgtt tgccccgtat tttcaaacgc gttacacttg ccgccgctgt tttcagccat
     9661 ttgattaccc gcaaccgccg tcattgcgcc ggcggtttgc ctgtcagcgt cattgcgccg
     9721 ctgtaaatac gaaagaacac attatgaccg tatcccccgt cgccttgcgc cgtaagaccg
     9781 agtgcaagcc tcatcccacc gcgcgctatt ggaaaaaatg cgatgtcgag gcacttttcg
     9841 gactgccgtt tttagaactt gtccatcagg cggcagaagt ccaccgccaa aatttcaacc
     9901 cgcgcgaaat ccagctttcc acgctgttgt ccatcaaaac cggcggttgt cccgaagatt
     9961 gcgcctactg tccgcaatcg gcgcaccaca ataccaatct gggcaaagag cagatgatgg
    10021 atgtggatga aatcgtcgaa aaagccaaaa tcgccaaatc gcgcggcgca agccggtttt
    10081 gtatgggcgc ggcatggcgc ggccccaaac ccaaagacgt ggagacggtt tccgcaatca
    10141 tcaaagccgt caagggcttg ggtatggaaa cctgcggcac gttcggtatg ctcgaagaag
    10201 gtatggcgga agacttgaaa gaggcgggct tggattatta caaccacaac ctcgacaccg
    10261 accccgaccg ctacaacgac atcatccata cacgccggca tgaagaccgt atggacacct
    10321 tgggcaaagt ccgcaacgcc ggtttgaaag tctgctgcgg cggcatcgtc gggatgaacg
    10381 aaacccgcgc cgaacgcgca gggctgattg ccagcctcgc caacctcgac ccgcagcccg
    10441 aaagcgtgcc gattaaccgg ttggtcaaag tggaaggtac gccgcttgcc gatgccgagg
    10501 atttggactg gacggaattt gtccgcacca tcgccgtggc gcggattacg atgccgcaaa
    10561 gttatgtccg actgtcggca gggcgcagca atatgccgga agcgatgcag gcgatgtgct
    10621 ttatggcggg cgcgaactcg attttttacg gcgacaagct gctgaccacg ggcaatccgg
    10681 atgaagacgg cgacagaatc ctgatggaaa agctcaacct gtatccctta cagtttgagc
    10741 tggaaggcga ggtcgccgaa gtggaaaaag cctctgggat taaagtggat tattgacgat
    10801 tgcaaaaatg ccgtctgaaa cccgaaaaaa ggctttcaga cgacatttgt tcggacggta
    10861 tttccaatat ctttttaccg gcgcgtgatg ttgccgtcgg gcgagacatc cagcccgttc
    10921 ccgttgggga agtcgctgcg gttcagataa ataatccgcc cgataacggt ttttcagggt
    10981 cgattttggg gattttatcg tcggcttggg taatagggat gatagttccg gaaataaatt
    11041 taccctgttt gttggtgata attttgaaaa tcggcgcaat gccgctgatg ccggaggtgt
    11101 tgattgcgcc gtaggtggca aagttgccgc cgctgtaaga gatgaagcgg tcgcggtaaa
    11161 gttcgacggc gcgtgtaacg tgcggcccct gcccgaatac gacatccgcg ccggaatcga
    11221 cggcaagccg tgcaaactcg acgacgttgc ccctgttttc cccatagaag atttcggtat
    11281 cgaacggcag gtgttccgcc tgtttccctt cccgccgccg tggaacatta caatgacgat
    11341 gtcggctttc tgtttggttt tggtaatccg ttttctaact ttggcataat cgttcagttt
    11401 gacggcggca aggttggggg cgaaggagac gaagccggat cttacgccgt ttttcttcag
    11461 gatggcggtt tcaaacctgt tttcgatgcc cgaatatttg atgttcaatt cgtcaaggtt
    11521 cgccctttat gccgtttccg caccgcaaac cgccggggtc aagccctcgg cgcatcctgc
    11581 cggaacggaa tccccgtgct gccgattgac ggttcaaacc ccgcgcccgt ttcaaatacc
    11641 ggcgatgtgg acggacagga tgcgcctgac gaaaagacag ccgataccgt ttccattatc
    11701 ggcgtgggcg acattatgct cggcagcaat tatccggtcg attacctgcc cgataccaat
    11761 attctgaaaa acgtcgaatc tgccttgcaa gacgcggaca ttaccgtcgg caacctcgaa
    11821 ggcacgctgt ttgacgaagg cggtacgccg aaaaaatgtg caacccccca aaatatgcta
    11881 tgcattccga acgccctccg catacgggca ataccttgcc gacgcgggat tcgactacct
    11941 cagcttcgcc aacaaccaca gcaacgactt cggcgcgcaa ggcatcacgg caacggcggc
    12001 gagcggcagc tcttttacat actcgatcgc gctaaagccg ctgccgataa cgaggccaaa
    12061 attgcggaaa ataccgccat cgcccagata aatttgtcca tcatcagacc tttactgttc
    12121 agacgagaca gcatttgccg cacgttttgg ggcttatctt tcgatttgcg ctacgtcgcg
    12181 caccgcgcct ttgtcggcgg aagtcgccat cgcgccgtaa gcacgcaggg ctgcggagac
    12241 gtagcggtcg cggttttccg gtttccacgc tttgctgccg cgcgcttcca tttcggcacg
    12301 gcgggcggca agctcttcat cggaaatggc aaggtggatg ctgcggttgg ggatgtcgat
    12361 ttcgacggta tcgccttcgt gtaccaaacc gatcgcgcca ccttccgccg cttcgggcga
    12421 ggcgtgtccg atggacaaac ctgatgtgcc gccggagaag cgtccgtcgg ttaagagagc
    12481 gcaggctttg ccgaggcctt tagatttcag gtaggaagtc ggatacagca tttcctgcat
    12541 gcccgggccg cctttcgggc cttcgtagcg gatgatgacg atgtcgccgg cgacgatttg
    12601 gttgcccaaa atgccttcta ctgcgtcttc ttggctttca aacacgcggg cgcggccggt
    12661 gaatttgagg atgctctcgt ccacgcctgc ggtttttacc acgcagccgc gctcggcgat
    12721 gttgccgaac aagaccgcca aaccgccgtc ttgcgagtag gcgtgtgcca cgtcgcggat
    12781 acagcctttt tcgcggtcga ggtcgagggt tttccacata cggttttgcg agaacgcttg
    12841 ggtggtgcgt acgccgccgg gcgcggcttt gaagcgttcg atggcgcggg tgttttcagg
    12901 gttggttatg tcccattttt caatcgcgtc tttcagcgtc ggtgcgtgga tagtgtgcac
    12961 gtcggtgtgc agcttgcccg ctttgtccaa ttctttcagg atggcgaaaa tgccgccggc
    13021 gcggtgcacg tcttccatat agtagtcgtg gttgttgggt gcggttttgc agatgcaggg
    13081 cacaacgcgg cttaagcggt caatgtcggc cattttgaaa tcgacaccgg cttcgttggc
    13141 aacggccaac aggtgcaaaa tggtattggt gctgccgccc atcgcaatat ccatcgtcat
    13201 agcgttttca aacgcttttt tggtggcaat gctgcgcggt aacacggttt catcgttttg
    13261 ctcgtaatag cgtttggtga tttcgacaat catacggccg gcttcgagga acagttcttt
    13321 gcggccgacg tgggtcgcca aatacgaacc gttgccgggt agggacagac cgagtgcttc
    13381 ggtcaggcag ttcatcgagt ttgccgtaaa catacccgaa cacgagccgc aggtcgggca
    13441 ggcgttttgt tcgacttcct cgacttgccg gttgctgaca ttgtcgtccg ccgattcaat
    13501 catcgcgtca atcaagtcca aacggcgttc gggctggatg tttgccacgc cgataacttt
    13561 acctgcttcc atcggaccgc cggaaacgaa gatggtcggg atgttcaggc gcatcgcggc
    13621 aatcagcatg cccggggtga ttttgtcgca gttggaaatg cacaccagcg cgtcggcgca
    13681 gtgggcattg accatatatt cgatggagtc ggcaatcaaa tcgcggctgg gcagggaata
    13741 cagcataccg ctgtgtccca tagcgatgcc gtcgtcgatg gcgatggtgt tgaattcttt
    13801 ggcgatggcg cccgcctttt cgatttcgcg ggcaaccagc tggcccatat tgtgcaggtg
    13861 gacatggccg ggcacgaatt gggtgaagga gttggcaacg gcgatgatgg gcttgccgaa
    13921 gtcggtttcc atcacgccgg tggcgcgcca caatgcacgt gcgcccgcca tattgcggcc
    13981 gtgggtggag gttttggagc ggtattctgg catagtgtgt ttccttgtct gctatgccgt
    14041 ctgaacggca aaaggctttc agacggcatt tttgttgtgt tttgaaatga aaaaaaaggt
    14101 attttatacc aagtatcgga attttgcgga atggaaacgc gtgcggcaaa aaaggacatc
    14161 ccccgcagga atgcggggga cggtttcagg cgcgcgcaat cgggacggct ttggatgcgt
    14221 cccaaaaatc gacgggcgca tccaacacgg gtttgacgat gcccgtccgt atggcgaaat
    14281 cgccgaaacc ttcgtcggta tcgcgtcccg ccgcccattt gccgatcagg tcgtctaatt
    14341 cggaaaggat ttccggcagg gtaatgtttt ctttgtagag tcgggggatg cgtacgcctt
    14401 cgcggtcgcc gccgatgtgg aggttgtagc gtccgacggc tttgccgacc agtccgattt
    14461 ccgccaacat cgcccgtccg cagccgttcg ggcagccggt aatgcgggta acgatgtagt
    14521 cgtccgacgt gccgtgtttc gccataatct tatccagctc gccgatgaag tccggcagca
    14581 cgcgttcggc ttccgccatt gccagcgggc aggtcgggaa ggaaacgcac gacatcgcat
    14641 tttcacgcag tttactgaca tcgttgcgga ttaatccgta tgttcgggca agttcttcga
    14701 tttttgcttt gtctgcttcg gcgacatttg ccacgatgag gttttggttg gcggtgatgc
    14761 ggaaatcgcc tttgtggatt ttggcgattt ccaacacgcc ggtcagcagc tgtttcccgc
    14821 cttcgtcaac caaacgcccg ctttcgatga aaagggtcaa atgccagttg ccgtctatgc
    14881 ctttcaccca gccgatacgg tcgccgcgcc cggtaaattt gaacgggcgt atgggttcga
    14941 acggcatacc catacggcgt tcgacttccg cgcggaagtt gtccaagccc atattttgaa
    15001 tggtgtagcg ggtgcgggcg tttttgcggt cgctgcggtt gccgaagtcg cgctgcgtgg
    15061 ttaccaccgc ttcggcggcc ttcagcgcgt gttccggagg cacgaaaccc agttccagtg
    15121 aaatgttcgg ataggttttg gtgttgccgt gttccatcga aagcccgccg cctgccaaaa
    15181 cattgaagcc ggcaagctgt ccgttaccgt ctgaaacggc gacgaaatcc aaatcgttgc
    15241 cgtagcagtc cacatcgttc aagggcggga tgacgactgc ggttttgaat ttgcgcggca
    15301 gataggtttt gcccaaaatc ggctcgtctt cttgaaggaa gtcgtcggaa ctttgaactt
    15361 ttttgccgtc cacccacaca tccagataac cgcgcgtgcg cggcagcaag tgttcggaaa
    15421 tctttttcgc gtattcgtaa gcctgccggt gcagttcgga ctcgatcggg ttggacgtgc
    15481 aaagcacgtt gcggttcata tccgccgccg tggcgatgga atccaaaccc agtttgtgca
    15541 agaggcggtg catcgtctgc aacttggctt tcggcacgcc gtgaaattgg aaggtttgcc
    15601 ggttggtcag ccggatggag cggtaatgac tgttttcccg ggcaaatttg tccagttcta
    15661 tccattggga cggtttgatg atcccgcccg gcagccggca gcgcaaaagc ataaatttca
    15721 agggctcgag ttttgcctcg gcgcgttcgg cgcggatgtc gcggtcgtcc tgctcataca
    15781 taccgtggaa gcggatgagt tggaagttgt cgcctttgaa gccgcccgtg agcgggtctt
    15841 tcaaatcgtc caaaatcgtg ccgcgtaaaa aattgctttc ggttttcaga cgttcgttgt
    15901 cggatagcgg tttttcttgc cacgccaaac cttttgtctt ggtctgtacg gtcattttgt
    15961 gttcctcccg attatattta atcaataaac atcacgctga tagcgttttt cttcgcgcag
    16021 catatccaaa tattcttctg cgccctcttc gtccaaatgt cctgccccga taatcacatc
    16081 cagcaaggcg gcttccacgt cttttgccat ttttgccgca tcgccgcaca catagatatg
    16141 cgcgccttcc tgcagccatt gccaaagtcc ttccgcctgt tcgcggattt tgtcctgcac
    16201 atagattttt tcttcctgat cgcgggacca ggcgaaatcg tacctgtgca ggaaaccgtc
    16261 tttggcaaac tgctgccatt cggtttgata gagaaaatca cgggcaaaat gcggattgcc
    16321 gaaaatcagc cagtttttgc cttccgcatt ttctgcggca cgttgttgga cgaaggcgcg
    16381 gaacggtgcg acgccggtgc ccgagccgat catcacaatc ggcttgcggc tgtcttcggg
    16441 cagcctgaag ccgtcgttgc gttccacaaa cacgcgcacc gtgccgtcct cttccagccg
    16501 gtcggcaagg aaacccgatg cgccgcccgc cctggcgcgc ccttcgtgtt cgaaacgcac
    16561 cgcgccgacg gtcaggtgca cttcgtcccc cacttccgcc tgcgacgagg aaatcgaata
    16621 caggcgcggc gcaagcgggc gcagtaggcc ggcgaattgt tccgccgtca gttttgccgg
    16681 gaagcggtgc agcacatcgg caatcggcgt gctttgcaca aagccttgca aaacggcgtt
    16741 gtcggcagca atacggtcga gttcgtcatc atcggcgaac ggggcatagc ctttgacaaa
    16801 ggcgggggtg ttttgcgtga gttcaaaatg ggataacagt gcggaggcaa ccggcagggt
    16861 ttttccgccc gcctgtattt ccgttgccgg atcgatgccg agcaggtcta ggatttccct
    16921 gaccagtgcc ggatcgttgt caaaccaaac gccgagcgcg tcgcccggga ggtagtgcaa
    16981 atccgaaccg ctcaaatcga tttcgatgtg gcgcacgtct ttatcggatt ggcgggcggt
    17041 gattttctga ttggccagca gggcggcggg aaaggggtct gccttgcagt acctgccatc
    17101 cggtgccgtc tgaaggccgg cggggggcgt tgtctgcggc gcgggcgttg cccggttttt
    17161 tgcggcttct tcttttaagc gcgcgacaat gcggcctgtc catccgtctg cggcggcggc
    17221 aaagtccaaa tccgcatcaa cgcgttcgag caggcgtttt gcgcccaatt cttcaaaacg
    17281 ccggtcgaaa tctttgcccg cccggcagaa attcggatag gaactgtcgc ccaaacccag
    17341 tacggcaaat tggagtttgt ccaatttcgg ggcttttttg ccgttcagca gtttgtgcag
    17401 cacgacggct tcttccggcg gttcgccttc gccctgggtg gacgtaacca gcagcaggcg
    17461 gcgttcgtcg gcgatgtttt tcgccttata gtctttcagt tcggcgcgcc tgacttggat
    17521 gccggcggct tccaggctgt ccgacgcttt gtcggcaacg gatttcgcat tgccggtttg
    17581 cgaggcggaa aggacggtta cggaaaaagg ttctgccgcc ggcaatgccg tctgaagcgc
    17641 gggcagccct gcggatgccc cgtttcctgc ttttgcccaa gcgtagccgg acagccacgc
    17701 ccattgtgcc gcgtccagcc ccgacaggag ctgcgtgatt tcgggcggca gaggcggtaa
    17761 tggcggattt gtgttctgca tatcgtgttc actcataaaa tcatacctgc cgcaacagtg
    17821 ccgtatgtcg cttcgtctat caggataaac gaaccggcgg cggtgttttc cgcataaggc
    17881 gttgccgtaa cgggtttttg aaggttgatg cggactttgg cgatgtcgtt catcttcaag
    17941 gattccgcgc cggcctcttg ttccagcgtg cggacatcta aaacgctttc aatttccccg
    18001 acttttgccg gcacggtttg cgtgccgtgc ttgagcaggt atttgcgcgc ggtgttgagc
    18061 ggacgttcgt caaaccagca aagcgtggct tccagatgtt tttgcggggc gagcggggaa
    18121 tttttatcga caaaaaggtc gccgcgcgaa acatcgatgt cgcggtccag ccggagggtt
    18181 gccgcctcgc cggcaaaagc ctgcgccact tcccctttcg gcgtgatgat ttcggacact
    18241 tcggcggtca gcccgttcgg ttcgatgcgg acggtttgcc cgacggcgac cgaaccgcgt
    18301 tcgatgcgcc cctgatagcc tcggaaatca tcggccttgt cggcatcttg gcggacgacc
    18361 agttggacgg ggaaataaaa atcggcagcg gaacgaccgg tttcgtccgc ccccggcagg
    18421 ttttccaaga tgaccagcag cggttcgccc cgataccacg gcatattgcc gccggggtaa
    18481 acgaggttgt cgcccaagag tgcggacacc ggtacgaaat gcacgtcttt caaaccgagc
    18541 tgttcggcaa gtcggcggta tgcctccaca atggcgttga atttgtcttc gctgtaatcc
    18601 agcaggtcca ttttgttgac cgccaccaca atatgcgggc agttgagttg gcggaggatg
    18661 gcggaatggc gtttggtctg cggcagaagc tgcaagggct gcgcgccgaa atccagttgg
    18721 gatgcgtcaa ccagcacgac tgccgccgaa gcggtgcttg cgcccgtaac catattgcgc
    18781 gtgtattgtt cgtgccccgg cgtgtcggcg atgatgaatt tccgtttcgc cgtggaaaaa
    18841 tagcggtatg ccacatcgat cgtaatgccc tgttcgcgtt cggcttccag tccgtcggtc
    18901 aggatggaga agtctatggt ttctttcaaa cctttgcttt tgccggattc caaggttttg
    18961 atttggtcgg acagcagggc tttgctgtcg tagagcagtc gtccgatcag ggtgcttttg
    19021 ccgtcatcga cgctgccggc ggtaatgaag cggagcgggg cctggtgttg tgccgtcata
    19081 ttttcttcct catatctgct taaagggttt ttgaaattta gaaatagcct tcttttttgc
    19141 gtttttccat tgccgcctcg cttgcctgat cgtctagccg ggtcgcgctg cgttcggaaa
    19201 tgtcggcaac cgccgtttcc ctgataatct ccgccggcgt ggacgcggtg ctttctaccg
    19261 ggcaggtgca gctgatgtcg ccgacggtgc ggaagcggac atcaaggatt tcggaggttt
    19321 cagacggcat tttcggggtg agcggcgtta cagggaccag cagccccctg cgtctgacca
    19381 cttcgcgcct gtggctgtaa taaatcggcg gcagctcgag gttttcgcgg gcgatgtatt
    19441 gccagatgtc gagttccgtc cagttggaaa tcgggaagac gcgcatattt tcgcctttgt
    19501 gcagcctggt gttgtacagc gaccacagct cggggcgttg cgccttcgga tcccattgtc
    19561 cgaactcgtc gcggaacgag aaaatccgtt ctttggcgcg ggctttttct tcgtcgcgcc
    19621 gcgcgccgcc catcagcgcg tcaaagccgt ttgcctcgat ggtttccaac aaggtaaccg
    19681 cctgtgccgc attgcgcgaa tcggtttctt tgcgtaagac caccgtgcct ttagcgatgg
    19741 agtcttccac gcgccccact atcaggcggg cattgagttt tgccgcctgc gcgtcgcgga
    19801 aggcaatcac ttcggggtag ttgtgccccg tgtcgatatg caccagcggg aagggcagtt
    19861 ttaccggcct gctgcccagc cggaaggctt tgcaggcgag ggcgagcagg accacggaat
    19921 ctttgccgcc ggaaaagagc agggcggggt tttcgcattc tgccgccact tcgcggataa
    19981 tgtggatgga ttcggattcc aaccagtcga gttggacgtt gttcggttcg gttttcgtca
    20041 tacgatattc cttatttctt ctgtttgatc cttatgaatt atttgtgcag cccgcattct
    20101 ttgctgtttt tatcttccca ccaccaccgc ccggcgcgga tgtcttcgcc ggccttgacg
    20161 gggcgggtgc aggggtcgca gcctatactg ggaaagcctt gccggtacaa atcgttgtaa
    20221 ggcacattgt tggcaaggat gtatgcccac acgtcgtgtt ccgaccagtc gaaaatcggg
    20281 ttgtatttat cgatgccgcg cccggcatca tattcggcaa acggcagttc cgtgcgtgtg
    20341 gcggactgtt cgcggcgttg cccggtaagc caagcatccg ccccggcaat ggcgcggtcg
    20401 agcggttcgg tttttcggat gcggcagcat tcgcggcgcg cttcaacgct gtcgtaaaag
    20461 gcaaacctgc ctttgctttc cacataatgc agggcatctt cccgtatcgg ttggaaacgc
    20521 ttgatttgca tatgcggata aacacgttca atcctatcca acaggtttaa agtttccgcg
    20581 tgcagcagcc ccgtatccaa agtaaaaata ccgatattga ggttttctcc ggcgataagg
    20641 tcggtaatta ccatatcttc cgcagcaagg ctgctggcaa accgtgcatc ccggtggctg
    20701 ccggcaatcc ggtgcaggcg ttgtttgagg gtttccgttt tttccgccaa agccgtttca
    20761 ctgccatttt caatcggcgg aattttccat aaagcaggtt tgaaaatttt cattttgtcc
    20821 gccttgcttt atgcggcttg ttgttcttgt ccggtgccca gtccgtccaa tgcaaaatac
    20881 gcgtgttcgg ccggattgct ttcttttttg gcgttttcgc cgaaccaagc cagtttttcg
    20941 tgcagcccca ccacttcgcc tatgacaatc aatgccggat tcggcgcggt ttcggcgagt
    21001 tcggcaaggt tggcgagcgt gccggttgcg gttttttgag ccggcagcgt gccttggctg
    21061 ataacggctg ccggcgtgtc gggcgagcgt ccgtgctgtt gcagccgttc ggcaatcagg
    21121 gcggctttga gcgcacccat ataaatcacc aaggtctggc ggctgcgggc gagggtctgc
    21181 cattcgatgt cgggcgcatc cgccttgcgg tggccggtta cgaaaaccgc actttgggca
    21241 taatcgcggt gcgtgagcgg gatgccggca taggcggtcg cgccgacggc ggcggtaatg
    21301 ccggggacga ccgaaaacgg gatctgatgg cgtgccaagg tttccaattc ttcgccgccg
    21361 cgtccgaaca cgaaagggtc tccgcctttt aaacgcacca cgcgcctgcc ttcgcgggcc
    21421 agcctgacca taagcgcatt ggtgtcctct tgcggggtgc ggccgccccg ggcgcgcttg
    21481 ccgacaaaaa tccgttccgc atcgcggcgg acgagggaca gtatgccgtc tgaaaccagc
    21541 gcgtcgtaaa gcaccacgtc tgcctgctgg atttcctgca gccctttgag cgtcagcagc
    21601 cccgcatcgc cgggacccgc accgaccaaa gacacaaaac cgccttgata attttgttcc
    21661 aactgctttg ccaattcccg ttcggcaagg gtgttttgcc ggtttttgac gagggcggcg
    21721 aaacgtccgt taaactgctt ttcccaaaag cggcggcgtt cggtaacgga tttcagtttg
    21781 cccttgacgg catcgcgcca ccttcctgaa atttccgcca tatcgcccaa agacggcggc
    21841 agcagggctt ccagcctttc acgcagcagt cgggcgagga cgggcgcgct gccggagctg
    21901 gaaacggcaa tctgaaccgg gttgcggtcg ataaccgacg ggaagatgaa gctgcaatgg
    21961 tcgcggtcgt ccaccacgtt gaccggcttt tggcagcttt cggcaagatg gaaaacgcgc
    22021 cggttgaggg cttggtcgct gcttgccgca atgatgagga aaaccgtgcg gatgtgttcg
    22081 gcacgaaatt cttcggcaag ccacaggatt ttgttttccg ccgccaacgc ggagagttcg
    22141 gcattcaggt gttttgcggc aaccctgacc tctgcgcccg ccttcagcag caggctgatt
    22201 ttgcgtgcgg cgaccgcgcc gccgcctacg accaatacgg ggcggccggc gaggttggcg
    22261 aaaataggga aataattcac tggctgactc ctttgctgtt tgcccgcacc ttgtttccga
    22321 tacggtgcgt cgcggcattt ttgtcggaat gcgggtcatt ttagacaaaa ggattttccc
    22381 ggttaaataa taaaaaggca tttgttagaa gctgaaagct atatgagggc ggctgcggat
    22441 gcggcggttt
//
LOCUS       DAWKST010000011        21822 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.011, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000011 DAWKST010000000
VERSION     DAWKST010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21822
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.011"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(87..860)
                     /locus_tag="WJL87_000296"
     CDS             complement(87..860)
                     /locus_tag="WJL87_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ7798784.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVKSATVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETAIPRNI
                     RLAEAPSHGMPVMAYDAQAKGTKAYLALADELMARVSGK"
     gene            complement(959..1582)
                     /gene="rsmG"
                     /locus_tag="WJL87_000297"
     CDS             complement(959..1582)
                     /gene="rsmG"
                     /locus_tag="WJL87_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ7798785.1"
                     /translation="MERKERLRAGIAAMGLDISETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTVHLLKDG
                     GYWAAMKGVYPQEEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            complement(1678..2163)
                     /locus_tag="WJL87_000298"
     CDS             complement(1678..2163)
                     /locus_tag="WJL87_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798786.1"
                     /translation="MFVNEKYPYAALFAGLVFLTLPFALAVHDAFALAFGRTGLLVSV
                     SDGGFGWRGGWDGVVWFVFGVFAFLNVVVSAGLTKLAYKKMMRRHSRYTLFLSGVAAC
                     AAAAVAWIFELLLGSGALGGLRGEAVLEYAFAVWLVAMLTLPKRLTRAPVQPVVFHRK
                     K"
     gene            2268..2343
                     /locus_tag="WJL87_000299"
     tRNA            2268..2343
                     /locus_tag="WJL87_000299"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2301..2303,aa:Thr,seq:cgt)
     gene            2376..3542
                     /locus_tag="WJL87_000300"
     CDS             2376..3542
                     /locus_tag="WJL87_000300"
                     /inference="COORDINATES: protein motif:HMM:NF016513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ7798787.1"
                     /translation="MLFHISASDGTTDMNTSQRNRLVSRWLNSYERYRYRRLIHAVRL
                     GGAVLFATALARLLHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGL
                     GVLWLNQHYFHGNLLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWF
                     DSGLMRAMNVLIGAAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTR
                     ERLEENMAKMRQINARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTT
                     AAKLQSPKLNGSEIRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAE
                     HLHYQWQGFLWLSTNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            3723..4280
                     /gene="frr"
                     /locus_tag="WJL87_000301"
     CDS             3723..4280
                     /gene="frr"
                     /locus_tag="WJL87_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ7798788.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            4336..5082
                     /locus_tag="WJL87_000302"
     CDS             4336..5082
                     /locus_tag="WJL87_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ7798789.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            5085..5882
                     /locus_tag="WJL87_000303"
     CDS             5085..5882
                     /locus_tag="WJL87_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ7798790.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMSGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAIMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADVCAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTSVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSKLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            5887..7122
                     /locus_tag="WJL87_000304"
     CDS             5887..7122
                     /locus_tag="WJL87_000304"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ7798791.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAVQCRTFRPEYAVVADAEHAARLEALLKGDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDSITPA
                     QAVKHPNWSMGRKISVDSASMMNKGLELIEAHWLFNCPPEKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            7157..8497
                     /gene="rseP"
                     /locus_tag="WJL87_000305"
     CDS             7157..8497
                     /gene="rseP"
                     /locus_tag="WJL87_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ7798792.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEADKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLTYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            8554..10947
                     /gene="bamA"
                     /locus_tag="WJL87_000306"
     CDS             8554..10947
                     /gene="bamA"
                     /locus_tag="WJL87_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ7798793.1"
                     /translation="MKLKQIASALMVLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEIGYANGYGKTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDVRTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            11013..11513
                     /locus_tag="WJL87_000307"
     CDS             11013..11513
                     /locus_tag="WJL87_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ7798794.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            11546..12589
                     /gene="lpxD"
                     /locus_tag="WJL87_000308"
     CDS             11546..12589
                     /gene="lpxD"
                     /locus_tag="WJL87_000308"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ7798795.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKAHGGI
                     HPTAVVEPSATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCKLGDEVVMHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVHIHRLSEMNKRLK
                     TLEQQLSDGKDTQ"
     gene            12625..13074
                     /gene="fabZ"
                     /locus_tag="WJL87_000309"
     CDS             12625..13074
                     /gene="fabZ"
                     /locus_tag="WJL87_000309"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ7798796.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVS
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAIIMCAKRVV"
     gene            13281..13961
                     /locus_tag="WJL87_000310"
     CDS             13281..13961
                     /locus_tag="WJL87_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEZ7798797.1"
                     /translation="MERYKNAVGEDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CLQQAFKDRNWLYNSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDATGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINKFRNRLAHHEPICFKGAIKDTNYARNIHQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            14004..14780
                     /gene="lpxA"
                     /locus_tag="WJL87_000311"
     CDS             14004..14780
                     /gene="lpxA"
                     /locus_tag="WJL87_000311"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ7798798.1"
                     /translation="MTLIHPTAVIDPKAELDSSVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRHAETQAELAVFRDFFAQSARGIIR"
     gene            15138..16529
                     /locus_tag="WJL87_000312"
     CDS             15138..16529
                     /locus_tag="WJL87_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ7798799.1"
                     /translation="MQVFLDNPKAFFETVSGWVWGPVMLMLLVGTGILLTVLLKGLQF
                     TMLGYALKQAFVPPKKHKSGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVHETR
                     DFKQKITNGELPH"
     gene            16608..17864
                     /locus_tag="WJL87_000313"
     CDS             16608..17864
                     /locus_tag="WJL87_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ7798800.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWQMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEERKKGTLQIFRQTKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCHLFTENLYKLCQEKGVQF
                     HFNQTISRIDHNGLRIKAVETETGRFEADAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQALFWSGLRPMTPDSTPLIGRTRFENLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            18045..18854
                     /gene="zupT"
                     /locus_tag="WJL87_000314"
     CDS             18045..18854
                     /gene="zupT"
                     /locus_tag="WJL87_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ7798801.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            18951..21788
                     /locus_tag="WJL87_000315"
     CDS             18951..21788
                     /locus_tag="WJL87_000315"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ7798802.1"
                     /translation="MLDKYNPAEIESKHYQNWEEQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFDLEAKVLANAEVFNFKGEAQPGFALPEKYAGLDRFAARK
                     QMVADLQEQGLLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGTVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQAEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIDLDKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGYSFPDMWIVGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADGGDIVQTAFEQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADYLKYLPMMTRLIEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQTQLSKLKD"
BASE COUNT         5357 a         6454 c         5537 g         4474 t
ORIGIN      
        1 cgatataagt atttttattt taatccgcca tattaacgca ctcggccaaa cagcataaag
       61 gcacgggcag cccgatttgg attgacctat ttccccgaca ccctcgccat cagctcgtcc
      121 gccaaggcaa gatacgcctt ggtacccttt gcctgcgcgt cgtaagccat aaccggcata
      181 ccgtggctcg gggcttccgc aagacggata ttgcgcggga tggcggtttc aaaaagcaaa
      241 tccccgaaat ggctgcgcaa ctgttcgctg acttcggcaa ccagcctgct gcggctgtcg
      301 tacatcgtgc gcacgatgcc cgtgatgtcc aaatcgggat tgaccgcctg acggattttg
      361 cgcacggttg caatcaaatc ggaaatccct tccagcgcgt aatattcgca caacatcggc
      421 acaatcacgc cgcccgccgc caccaagccg ttaagcgtca acagcgtcag cgaaggcgga
      481 cagtcgatca ggataaagtc gtaatcttcc gccactgcct tgagtgcgtt tttcaaacgc
      541 acttcccggg cgatttcctg caccagctcg atttccgcac cggccagcgc gcggttcgca
      601 cccaacacag cgtatccgcc ctctttgctg cgtaccgtcg ccgatttcac gtccgcatcg
      661 cccaataaga cctgataaac gccggactgc aaacccgcct tgtcgatacc gctgcccgtc
      721 gtcgcattgc cctgcggatc caaatcaacc accagcacgc gtttgccgcg cgatgccagc
      781 gaagccgcca aatttaccgt cgtcgtcgtt ttgcccacac cgcccttctg attggcgatg
      841 gcaaggatgt tcgcactcat gtcccgcccg tgtcccgtct gatgttgaaa aaagaaaaac
      901 agtgcggaat tttaccttta tccgcacaaa aaaggtattc atgccgtctg aagtgcgctc
      961 aacgcttgct caggatgacg atatggcgtt ccgcatccaa gcccggcacg tcgagccttt
     1021 ggactttttc aacgcacaca tcctgcggca ggcggccgat ttcttcctgc ggatacacgc
     1081 ccttcatcgc cgcccagtag ccgccgtctt tcaacagatg caccgtccac gacacaaaat
     1141 ccgccagttc tgcaaacgca cggctggtaa ccacatcggc acgcacgtcc gaaaccgcct
     1201 ccacgcgtcc ggataccacg cgcacattgt ccaaccccaa ctcgataacc gcctgctgta
     1261 aaaaagccgt tttcttcgta ttcgcatcca aaagggttat ctgcacatcc ggacggcaca
     1321 ccgccgccgg aatgccgggc tgaccgccgc ccgaaccgac atccagcatc gtttgcacac
     1381 cctcgatatg gggcagcagc gtcaggctgt ccaaaagatg atggacaatc attttttcct
     1441 cgtcgcgcag ggcggtcaga ttgtaggttt tgttccactt tttcaacaga tccacatagg
     1501 ccaaaagcct gtcctgtgcc gtttccgaaa tatccagccc catcgcggca atgcctgcac
     1561 gcaggcgttc tttgcgttcc atatccctat cctgtccatg ttcaggcgta atgttaacgg
     1621 aaaaacatgc cgtctgaaac cgcgtgtcgg ggttcagacg gcatttcccg ttccaaccta
     1681 ttttttcctg tgaaacacca ccggctgcac cggcgcgcgc gtcaggcgtt tgggcagcgt
     1741 cagcatcgcc accagccaca cggcaaacgc atattccaac accgcctccc cacgcagccc
     1801 gcccaaagcc ccactgccaa gcagcagctc gaaaatccaa gccaccgctg ccgccgcgca
     1861 agccgccacg cccgacagaa acagtgtgta acgcgaatgc cgccgcatca tctttttgta
     1921 cgccagtttc gtcagacccg ccgacacaac cacattcaaa aacgcaaaca ctccgaacac
     1981 aaaccacacc acgccgtccc agcctcctcg ccagccgaat ccgccgtccg acaccgacac
     2041 cagcaacccc gtccgtccga acgcaagcgc aaaggcatca tgcaccgcca acgcaaacgg
     2101 cagcgtcaaa aacaccagtc ccgcaaacag agccgcataa ggatatttct cgttcacaaa
     2161 catcgccgtc cccctcttcc gaagcagacc gcattatacc gcgcactgcc ttgccgcccg
     2221 ccagaaaagt tgcacaaaca accgttcata tatatcataa cgaaaacgcc ggtgtagctc
     2281 agtcggtaga gcagcgcatt cgtaacgcga aggtcggggg ttcgattccc ttctccggca
     2341 ccaataccaa gcacagaccc tccctcggga agcctgtgct ttttcacatt tccgcttcag
     2401 acggcacaac cgatatgaac acctcgcaac gcaaccgcct cgtcagccgc tggctcaact
     2461 cctacgaacg ctaccgctac cgccgcctca tccacgccgt ccggctcggc ggggccgtcc
     2521 tgttcgccac cgcactcgcc cggctgctcc acctccaaca cggcgaatgg ataggcatga
     2581 ccgtcttcgt cgtcctcggc atgctccagt ttcaaggggc gatttactcc aaggcggtgg
     2641 aacgtatgct cggcacggtc atcgggctgg gcgcgggttt gggcgtttta tggctgaacc
     2701 agcattattt ccacggcaac ctcctcttct acctcaccgt cggcacggca agcgcactgg
     2761 ccggctgggc ggcggtcggc aaaaacggct acgtccctat gctggcgggg ctgacgatgt
     2821 gcatgctcat cggcgacaac ggcagcgaat ggttcgacag cggcctgatg cgcgccatga
     2881 acgtcctcat cggcgcggcc atcgccattg ccgccgccaa actgctgccg ctgaaatcca
     2941 cactgatgtg gcgtttcatg cttgccgaca acctgaccga ctgcagcaaa atgattgccg
     3001 aaatcagcaa cggcaggcgc atgacccgcg aacgcctcga agagaacatg gcgaaaatgc
     3061 gccaaatcaa cgcacgcatg gtcaaaagcc gcagccacct cgccgccaca tcgggcgaaa
     3121 gccgcatcag ccccgccatg atggaagcca tgcagcacgc ccaccgtaaa attgtcaaca
     3181 ccaccgagct gctcctgacc actgccgcca agctgcaatc tcccaaactc aacggcagcg
     3241 aaatccggct gctcgaccgc cacttcacac tgctccaaac cgacctgcaa caaaccgtcg
     3301 cccttatcaa cggcagacac gcccgccgca tccgcatcga caccgccatc aaccccgaac
     3361 tggaagccct cgccgaacac ctccactacc aatggcaggg cttcctctgg ctcagcacca
     3421 atatgcgtca ggaaatttcc gccctcgtca tcctgctgca acgcacccgc cgcaaatggc
     3481 tggatgccca cgaacgccaa cacctgcgcc aaagcctgct tgaaacacgg gaacacggct
     3541 gacggtcgga cacgatgccg tctgaaacgc ctgccgcttc agacggcatc tccaatcctc
     3601 ctgccttgtc gtcatgcccg aataacggta ctattcgcag ttaacagttt tcatgccgtc
     3661 tgaaaggttt gaagcgcgtt tcagacggct gcccgcccac cttatcccac cgaaagaaca
     3721 atatgatcaa cgatattcaa aaaacagccg aaggcaaaat gcagcgttcg gtcgaagtac
     3781 tgaaagaaaa tctggcgaaa gtgcgtaccg gccgcgcgca taccggcctg ctcgaccaag
     3841 tggaagtcga atactggggc agcatggtcc ccgtcagcca agttgccaac gtaacgcttc
     3901 tggacgcgcg caccatcggc gtgaaaccgt ttgagggcaa tatggcggcc aaagtcgaga
     3961 aagccatccg cgattcaaac ttggggctga acccggcagc tgtcggcgac ctgatccgcg
     4021 tgccgatgcc catgctgacc gaggaacgcc gcaaagacct gattaaagtc gtacgcggcg
     4081 aagcggaaga aggccgcgtc tctatccgca acgtgcgccg cgatgccaac gaccacatca
     4141 aaaaactcct caaagacaaa gaaatttccg aagacgaggc acgtcgcggc gaagaagcgg
     4201 ttcaaaaact gaccgacaaa tacattaccg aagccgacaa actcctgact gccaaagaag
     4261 aagatttgat ggcaatttaa cctgcacggt tcggcgttca gacggcattt gaatgccgaa
     4321 ccgcgaaagg cagacatgaa aagcagcacg caggccgttt tggaacacac cgccattccc
     4381 aagcatatcg ccgtgattat ggacggcaac ggccgttggg cgaaaaaacg tttcctcccg
     4441 cgcataatgg gacacaaacg cggtttggac gcattggaaa atatggtgaa gcattgcgcc
     4501 aaactgggtg tgcaatatct gaccgtgttt gccttttcaa ccgaaaactg gcgccgcccc
     4561 gaagacgaag tttcgttcct gatggggctg tttttacagg ctttgcaaaa acaggtacgc
     4621 cgtctgcacg aaaacaatat gcgcctgaaa atactgggca gccgcgaacg cttcaaccgg
     4681 cagattctgc aaggcatcga agaagcggag gcgttgacgg caaacaatac cggcctgacc
     4741 ctgagcattg ccgccgatta cggcggccgc tgggatattt tgcaggcggc aaacaaactg
     4801 attgccgaag gcgtatccga gattacagaa gacacgctgg cgaaacactt gatgctgggc
     4861 gatgcaccgg aaccggattt gttcatccgc accggcggcg aaacgcgcat cagcaatttc
     4921 ctgctctggc agatggcata tgcagaactg tatttcaccg atattttgtg gcccgatttt
     4981 gacggcaagg ctttggacga tgccgtcgct tcgttccaaa aacgcgaacg gcggttcgga
     5041 cgcacctccg agcaactgcc tatcgaacag caaaggaact gaatatgctg aaacaacggg
     5101 tgataactgc tatgtggctg ctgccgctga tgctgggcat gctgttttac gcgccgcaat
     5161 ggttgtgggc tgcattttgc ggactgattg ccctgattgc cttgtgggaa tatgcccgta
     5221 tgagcggctt gtgcaaaatc aaaaccaacc attacctcgc cgcaaccttg gttttcggcg
     5281 tggttgccta tgcgggcggc tggatgctgc ctaatttggt ttggtatgtt gttttggcat
     5341 tttggcttgc catcatgcct ttatggttga gattcaaatg gaggctcaac ggcggttggc
     5401 aggtttatgc cgtcggctgg cttttggtta tgccgttttg gttcgcgctc gtatccctgc
     5461 gcccgcatcc cgatgatgcc ctgccgctgc tcgccgtgat gggtctggtc tgggttgccg
     5521 acgtttgcgc gtatttcagc ggcaaggcgt tcggcaaaca caaaatcgcg ccggcaatca
     5581 gccccggaaa aagctgggaa ggcgcaatcg gcggcgcggt ttgcgtggcc gtgtacatga
     5641 cctccgtacg aagtgccggc tggctggcat tcgatacagg ctggttcgat accgtgttaa
     5701 tcggtttggt gttgaccgtt gtcagcgtat gcggcgacct tttggaaagc tggctcaagc
     5761 gcgcggcagg catcaaagac agcagcaagc tactgccagg acacggcggc gtgttcgacc
     5821 gtaccgacag cctgattgcc gttatcagcg tctatgcggc gatgatgtcg gttttaaatt
     5881 gattctatgc cgtctgaaaa cgcttcagac ggcatccggt ataaagttat ccccattatg
     5941 acaccacaag tcctgaccat attaggcagt accggcagca taggcgaaag cacgctggac
     6001 gttgtctccc gccaccccga aaaattccgc gtattcgcgc tggcagggca taagcaggtc
     6061 gagaaactgg cggtgcaatg ccggacgttc cgccccgaat atgccgtcgt tgccgatgcc
     6121 gaacacgccg cccggcttga agctttgttg aaaggcgacg gcacggcgac gcaggtttta
     6181 cacggcgcgc aggcattggt tgacgttgcc tctgccgacg aagtcagcgg tgtcatgtgc
     6241 gccatcgtcg gggcagtggg gctgccttcc gcgctcgcag cggcgcaaaa aggcaaaacc
     6301 atttatctgg cgaacaaaga gacgctggtg gtttccggcg cgttgtttat ggaaaccgcc
     6361 cgtgcaaacg gcgcggcagt gctgcccgtc gacagcgaac acaacgccgt tttccaagtt
     6421 ttgccgcgcg attacgccgg ccgtctgaac gaacacggca tcgcttcgat tatcctgacc
     6481 gcttccggcg gcccgtttct gaccgccgat ttaaacacgt tcgacagcat tacgcccgcc
     6541 caagcggtca aacaccccaa ttggagcatg gggcgcaaaa tttccgtcga ttccgcgtca
     6601 atgatgaata agggcttgga gttgattgag gcgcattggc tgtttaactg tccgccggaa
     6661 aaactggaag tcgtcatcca tccgcaatcc gtgattcaca gcatggtgcg ttatcgcgat
     6721 ggttcggtat tggcgcagtt gggcaatcct gatatgcgta cgcctattgc ttattgtttg
     6781 ggtttgcccg agcgcatcga ttcgggtgtc ggcgacctgg atttcgacgc attgtccgcg
     6841 ctgaccttcc aaaagcccga ctttgaccgc ttcccctgcc tgaagctcgc ctatgaagcc
     6901 atgaacgcag gcggagccgc gccctgcgta ttgaacgccg ccaacgaagc cgccgtcgcc
     6961 gcctttttgg acggacagat taagtttacc gacattgcca aaaccgtcgc ccattgtctt
     7021 tcacaagact tttcagacgg cataggcgac ataggggggc tcttggcgca agatgcccgg
     7081 acacgcgcac aagcgcgggc atttatcggc acactgcgct gatgccgtct gaacaccgtt
     7141 atcaaaggaa aaccatttgc acacccttct agcttttatc ttcgccatcc tgattttggt
     7201 cagcctgcac gagttcggac actacatcgt tgccagattg tgcggcgtca aagtcgtacg
     7261 cttttccgtc ggcttcggca aaccgttttt cacccgaaag cgcggcgaca ccgaatggtg
     7321 cctcgccccg attccgttgg gcggttacgt caaaatggtc gatacgcgcg aaggcgaagt
     7381 atcagaagcc gatttaccct acgcttttga caaacaacac cccgccaaac gcatcgccat
     7441 cgtcgccgcc ggcccgctga ccaatctcgc actggcggtt ttgctgtacg gactgagctt
     7501 ttccttcggc gtaaccgaac tgcgccccta tgtcggcaca gtcgaacccg acaccattgc
     7561 cgcccgcgcc ggcttccaaa gcggcgacaa aatacaatcc gtcaacggca cacccgttgc
     7621 agattggggc agcgcgcaaa ccgaaatcgt cctcaacctc gaagccggca aagtcgccgt
     7681 cggcgttcag acggcatcag gcgcgcaaac cgtccgcacc atcgatgccg caggcacgcc
     7741 ggaagccgat aaaatcgcaa aaaaccaagg ctacatcgga ctgatgccct ttaaaatcac
     7801 aaccgttgcc ggtggcgtgg aaaaaggcag ccccgccgaa aaagcaggcc tgaaaccggg
     7861 cgacaggctg actgccgccg acggcaaacc catcgcctca tggcaagaat gggcaaacct
     7921 gacccgccaa agccccggca aaaaaatcac cctgacctac gaacgcgccg gacaaaccca
     7981 taccgccgac atccgccccg atactgtcga acagcccgac cacaccctaa tcgggcgcgt
     8041 cggcctccgt ccgcagccgg acagggcgtg ggacgcgcaa atccgccgca gctaccgtcc
     8101 gtctgttgtc cgcgcattcg gcatgggctg ggaaaaaacc gtttcccact cgtggacaac
     8161 cctcaaattt ttcggcaaac taatcagcgg caacgcctcc gtcagccata tttccggtcc
     8221 gctgaccatt gccgatattg ccggacagtc cgccgaactc ggcttgcaaa gttatttgga
     8281 atttttggca ctggtcagca tcagcctcgg cgtgctgaac ctgctgcccg tccccgtttt
     8341 ggacggcggc cacctcgtgt tttatactgc cgaatggata cgcggcaaac ctttgggcga
     8401 acgcgtccaa aacatcggtt tgcgcttcgg gctcgccctg atgatgctga tgatggcggt
     8461 cgccttcttc aacgacgtta cccggctgct cggttagatt ttacgtttcg gaatgccgtc
     8521 tgaaaccgca ttccgcacca caaggaactg acaatgaaac tgaaacagat tgcttccgca
     8581 ctgatggtct tgggcatatc gcctttggca cttgccgact tcaccatcca agacatccgc
     8641 gtcgaaggct tgcagcgtac cgagccgagc accgtattca actacctgcc cgtcaaagtc
     8701 ggcgacacct acaacgacac acacggcagt gccatcatca aaagcctgta cgccaccggt
     8761 ttctttgacg acgtacgcgt cgagactgcg gacgggcagc tcctgctgac cgttatcgaa
     8821 cgccccacca tcggctcgct caacatcacc ggcgcaaaaa tgctgcaaaa cgacgccatt
     8881 aagaaaaacc tcgaatcgtt cgggctggcg cagtcgcaat actttaatca ggcgacactc
     8941 aatcaggcag tcgccggcct gaaagaagaa tacctcgggc gcggcaaact caatatccaa
     9001 atcacgccca aagtaaccaa actcgcccgc aaccgcgtcg acatcgacat cacgattgac
     9061 gagggcaaat ccgccaaaat caccgacatc gaatttgaag gcaaccaagt ctattccgac
     9121 cgcaaactga tgcggcagat gtcgctgacc gaaggcggca tttggacatg gctgacacga
     9181 agcaaccaat tcaacgagca gaaatttgcc caagacatgg aaaaagtaac cgacttctac
     9241 cagaacaacg gctacttcga tttccgcatc ctcgataccg acatccaaac caacgaagac
     9301 aaaaccaagc agaccatcaa aatcaccgtc cacgaaggcg gacgtttccg ttggggcaaa
     9361 gtctccatcg aaggcgacac caacgaagtc cccaaagccg aactggaaaa gctgctgacc
     9421 atgaagcccg gcaaatggta cgaacgccag cagatgaccg ccgttttggg tgagattcag
     9481 aaccgcatgg gctcggcagg ctacgcatac agcgaaatca gcgtacagcc gctgccaaac
     9541 gccgaaacca aaaccgtcga tttcgtcctg cacatcgaac cgggccggaa aatctacgtc
     9601 aacgaaatcc acatcaccgg caacaacaaa acccgcgacg aagtcgtgcg ccgcgaattg
     9661 cgccaaatgg aatccgcgcc ttacgacact tccaagctgc aacgctccaa agagcgcgtc
     9721 gagcttttag gctacttcga caatgtccag tttgacgccg tcccgcttgc cggcacaccc
     9781 gacaaagtcg atttgaacat gagcctgacc gaacgttcca ccggctcgct cgacttgagc
     9841 gcgggctggg tacaggatac cggcctggtc atgtccgcag gcgtttccca agacaacctg
     9901 ttcggtacgg gcaagtcggc cgccctgcgc gcctcacgaa gcaaaaccac gctcaacggc
     9961 tcgctgtcgt ttaccgaccc gtacttcacg gcagacgggg tcagcctggg ctacgatgtt
    10021 tacggaaaag ccttcgaccc gcgcaaagca tcgaccagca tcaaacaata taaaaccacc
    10081 acggcaggcg caggcatccg catgagcgtg cctgttaccg aatacgaccg cgtgaatttc
    10141 ggtttggtgg cagaacacct gaccgtcaac acctacaaca aagcgcccaa acactatgcc
    10201 gactttatca agaaatacgg caaaaccgac ggcacagacg gcagcttcaa aggctggctg
    10261 tacaaaggta ccgtcggctg ggggcgcaac aaaaccgaca gcgcgttatg gccgacacgc
    10321 ggctacctga cgggcgtgaa cgccgaaatc gccctgcccg gcagcaaact gcaatactac
    10381 tccgccaccc acaaccaaac ctggttcttc cccctgagca aaaccttcac actgatgctt
    10441 ggcggcgaaa tcggctatgc caacggctac ggcaaaacca aagaaatccc cttctttgaa
    10501 aacttctacg gcggcggcct gggttcggta cgaggttacg aaagcggcac actcggtccg
    10561 aaagtctatg acgaatacgg cgaaaaaatc agctacggcg gcaacaaaaa agccaacgtc
    10621 tccgccgagc tgctcttccc gatgcccggc gcgaaagacg tgcgcaccgt ccgcctgagc
    10681 ctgtttgccg acgcaggcag cgtgtgggac ggcaaaacct acgacgacaa cagcagttcc
    10741 gcgaccggcg gcagggttca aaacatttac ggcgccggca atacccataa atccaccttt
    10801 accaacgaat tgcgctattc cgccggcggc gcggttacct ggctctcgcc tttaggcccg
    10861 atgaaattca gctacgccta cccgctgaag aaaaaaccgg aagacgaaat ccaacgcttc
    10921 caattccaac tcggcacgac gttctaatcc cgcaaatgcc gtctgaagcc cttcagacgg
    10981 catttcgcgg caacattcga aggagtttta ccatgacccg tttgacccgc gcgtttgccg
    11041 cggctctgat cggtttgtgc tgcaccgcag gcgcgcacgc cgacaccttc caaaaaatcg
    11101 gctttatcaa caccgagcgc atctacctcg aatccaagca ggcgcgcaag attcaaaaaa
    11161 cgctggacag cgaattttcc gctcgtcagg acgaattgca aaaactgcaa cgcgaaggtc
    11221 tggatttgga aaggcagctt gccgaaggca aactcagaaa cgcaaaaaag gcgcaagccg
    11281 aagaaaaatg gcgcgggctg gtcgcagcgt tccgcaaaaa acaggcgcag tttgaagaag
    11341 actacaacct ccgccgcaac gaagagtttg cctccctcca gcaaaacgcc aaccgcgtca
    11401 tcgtcaaaat cgccaaacag gaaggttacg atgtcatttt gcagaacgtg atttacgtca
    11461 acacccaata cgacgttact gacagcgtca ttaaagaaat gaacgcccgc tgaccctttc
    11521 agacggcata ccgaacagga aaaccatgat tccggccacc tacaccctgt cccaaatcac
    11581 cgcgcggctc ggcggcgaat ggcgcggcga ggacatttcc gttaccgccg tgcgcccgct
    11641 cgcagacgcg caggcggaac acatcagctt ccttgccaat ccgaaataca aagccgaagt
    11701 ccacgacagc agcgcgggcg cggtcatcgt ttccgccaaa gcggcagacg gatttgaagg
    11761 gcgcaacctg attgtcgccg acgaccccta tctctatttc gccaaagtcg cccgcctgtt
    11821 ttcacccgtc gtcaaagcgc acggcggcat ccatccgacc gccgtcgtcg aaccgagtgc
    11881 caccgttccc gcaagctgcg aaatcggcgc gaacgcctac atcggcgcaa acaccgtgct
    11941 cggcgaaggc tgccgcatct tggcaaacgc cgtcgtccaa cacgattgca aactgggcga
    12001 cgaagtcgtc atgcacccca acgccgtcgt ttattacggc tgtacactgg gcagacgcgt
    12061 cgaaatccac agcggcgcag tcatcggcgc ggacggtttc ggactcgcct tcgccggcga
    12121 ttcgtggttt aaaatcccgc aaaccggcgc ggtaacgctg ggcgacgacg tggaaatcgg
    12181 ctcgaacacc aacatcgacc ggggcgcgat gagcgacacc accgtcggca acggcaccaa
    12241 aatcgacaac caagtccaaa tcggacacaa ctgcaaaatc ggttcgcaca ccgtcatcgc
    12301 cgccaaaacc ggcatctcag gcagcgtaac cataggcagc tactgcatca tcggcggcgg
    12361 tgtcggcacg gtcggacaca tcgaaatcgc cgacaaaacc accatcggcg gcggcacgtc
    12421 cgtcacccac agcattaccg aaagcggcaa acacctcgcc ggcatcttcc cgatgtccac
    12481 ccataaagaa tgggcgcgca acgccgtcca tatccaccgc ttaagcgaaa tgaacaaacg
    12541 cctcaaaacg ctggaacagc agctttcaga cggcaaagac acgcaataaa tccaaaccga
    12601 ttttattcaa ggaatacgac agacatggac gtacaactcc ccatcgaagc caaagacatc
    12661 caaaaactca tcccccaccg ctatccgttt ctccagctcg accgcatcac cgccttcgag
    12721 ccgatgaaaa ccctgaccgc gattaaaaac gtcagcataa acgagcccca gttccaaggc
    12781 cactttcccg acctgcccgt gatgcccggc gtactcatca tcgaagcgat ggcgcaagcc
    12841 tgcggcacac tcgccatcct cagcgaaggc ggccgcaagg aaaacgaatt cttcttcttc
    12901 gctggcatag acgaagcccg tttcaaacgc caagtcatcc ccggcgacca actcgtcttt
    12961 gaagtcgagc tgctgaccag ccggcgcggc atcggcaaat tcaacgccgt tgccaaagtg
    13021 gacgggcaag tcgccgtcga agccatcatc atgtgtgcca aacgcgtggt ttgagcgttc
    13081 ggaaaaaggt cgtctgaaag tttcagacga cctaataaaa agaaaaccca ccggtttagg
    13141 tttattcaaa cccagggaca cggtggggat tagttttacc taactgtatc aaatatagtt
    13201 tgaaacgtca atttaaaaat aaagaatcag gtgttagcgt ggattataag gaatgctgta
    13261 gtattttttc tgatttcaga atggaacggt ataaaaatgc cgttggggaa gacaaagcag
    13321 ctgaattgta tttattgaat ttatctttat caagagaact ttttcatgtt gtttcaatat
    13381 ttgagattgt tttaagaaac aaaattgaca tttgccttca gcaggcgttt aaagacagaa
    13441 actggttata taacagcata cagccacaga cgaatcccgc attaaaatat caaggttgct
    13501 ttttaagaaa tggcacgaaa gaatctgctg aattaatcaa ggttgcatta tctaaaatac
    13561 aaaataatag cgggggaaag tttgaccaca accaattggt tgcaggacta ggattcggtt
    13621 tttggcggta tctgtttgca ggagggaaag atgcccaatt tgatgctaca gggaaagtat
    13681 tgatgaaagt ttttccaaaa aagcccaaat ctacaccaag tgtgcaatat aaccaaaaat
    13741 ggatttttcg agaactttcc aatattaaca aattccgtaa tcgtttggca catcacgagc
    13801 cgatttgttt taaaggtgca ataaaagata ccaactacgc tagaaatatt caccaatcta
    13861 tttttgaact gcttaattac atggatgtag atactgcttc agtatttagc cattttagcg
    13921 atcaagtgat tgcggtctgc gatgaaatcg acaaactgta attttctatt atttgtctta
    13981 acaagcagcg gagtattcaa aacatgaccc tcatccaccc gaccgccgtc atcgacccca
    14041 aagccgaact cgactccagc gtcaaagtcg gcgcgtacac cgtcatcggc cccaacgtcc
    14101 aaatcggcgc gaataccgaa atcggcccgc acgccgtcat caacggccac accagcatcg
    14161 gcgaaaacaa ccgcattttc caatttgcca gcctcggcga aatcccgcag gacaaaaaat
    14221 accgcgacga gccgaccaag ctgattatcg gcaacggcaa caccatccgc gaattcacca
    14281 cctttaattt aggcacggta accggcatcg gcgaaacccg tatcggcgac gacaactgga
    14341 ttatggcgta ctgccacctc gcgcacgact gcgtggtcgg caaccacacc atcttcgcca
    14401 acaacgcctc gcttgccgga cacgttacca tcggcgacta cgtcgttttg ggcggctaca
    14461 cgctggtttt ccaattctgc cgcatcggcg actacgccat gaccgcgttt gccgcaggcg
    14521 tacacaaaga cgtgccgccc tactttatgg catcgggcta ccgcgccgaa ccggcggggc
    14581 tcaacagcga aggcatgcgc cgcaacggtt ttaccgcaga gcagatttcc gccgtcaaag
    14641 acgtgtacaa aaccctctac catcgcggca ttccgtttga agaagccaag gcagacattc
    14701 tccgccacgc cgaaacccaa gccgagcttg ccgtattccg agactttttc gcacaatcgg
    14761 cacgcggcat catccgctga ccgtaccctt ttgatgccgt ctgaaacccc gacagcgttt
    14821 cagacggcat cggttttttt aaagcggtat attgtttgat cgatgcatca ttttgtagat
    14881 ttcagaagta tggcagtcgg cagcattgat gatttctgcc gcccgccaca tccccattcg
    14941 gacatttcat tgtgacctcc gccgctgctt ttaaagaatt gcaaacaatt tccgcagctt
    15001 gaaccggcct gttttcggcg aaaaaaacca aaagtatttt tcaaaagaac atattgtctt
    15061 attggatatt ctgtgctgaa attactggac actacaaaag tggtgtttta aaaataaaac
    15121 agacaaagga gaaagccatg caagtgtttt tagacaatcc caaagccttt tttgaaaccg
    15181 tcagcggctg ggtttgggga cctgttatgt tgatgctgct ggtcggcacg ggtattttgc
    15241 tgactgtttt attaaaaggt ttgcagttca cgatgttggg ttatgcgctg aaacaggcgt
    15301 ttgtgccgcc aaagaagcat aaaagcggcg aaggccacga aggcgatatt tcccatttcg
    15361 cggcgttgat gaccgcgctg tccgccacca tcggtacggg taacatcgcc ggcgtggcga
    15421 ctgcggtggt aaccggcggc ccgggcgcgg tattttggat gtggatgacc gccattttcg
    15481 gcatggccac caaatacggc gaaggcgtgt tggcggtgaa ataccgcgtc aacaattcca
    15541 aaggcgaaat gtccggcggc ccgatgtatt acatcgaaaa aggcttgggc aaaaactgga
    15601 aatggatggc cgtcgcgttt gcgctgttcg gcacattcgc ttccttcggt atcggcagct
    15661 cggtgcagtc caactcggtt gcacaggcgg tgcaaaccag cttcggtatc gaacctgcct
    15721 ataccggcat tacgttgacc gttctgactg ccgttgtcgt tttaggtggt attaaaggca
    15781 tcgccaaagc cgcttctttc atcgtgcctg ctatggcggt gttttatgtg ttgggcggtc
    15841 tttccattat cgcgattaat tccgatgcac tgatgcctgc cgtcaagctg attttctccg
    15901 atgcgttcag cgcgcaggca gtggcgggcg gtgctatcgg tacggtcatc cgctacggcg
    15961 tggcgcgcgg cgtgttctcc aatgaggcag gtatgggttc tgcgcctatt gccgccgcag
    16021 ccgcgaaaac cgaccatccc gtccgtcagg ccttggtttc tatgaccggt acgtttttgg
    16081 ataccatcgt cgtttgctcg attaccggca tcgtgttggt aatgggactg ctcggcgcgg
    16141 gcggcgagtt tgtcaaacct gaagtgagcg gcgcggcact gacaaccgtc acattccaaa
    16201 aaatgctgcc cggcatcggc ggctggattg tcaccatcgg cctgattttc tttgcctact
    16261 caaccattct cggctggtgc tattacggcg aaaaatgcgc ggtgtatgtt ttcggcgaga
    16321 aatttgccgg tttgtaccgc gtcggttatg tttcttccgt tatgctgggt accgtgttga
    16381 gccttgattt ggtgtggctg gcttcggaca cattcaacgg cttgatggca ttgcccaacc
    16441 tgattgcact tttattgatg gcgaaagtca tcgtccatga aacgcgcgac ttcaaacaaa
    16501 aaatcacaaa cggcgaattg ccgcattaat atccgaggcc gtctgaatcc gaatattcag
    16561 acggcacggc atattaagtc tgttttgaaa aagccggagg gagaaacatg aaagtgcttg
    16621 ttttaggtgc gggtgttgcc ggcgtgtctt ccgcgtggta tctggcagag gcaggacatg
    16681 aagtaacggt catcgaccgc gccgagggtg tggcgatgga aaccagtttt gccaacgcag
    16741 gccagctttc ttacggctat accacgcctt gggctgcacc cggtatcccg accaaagcgc
    16801 tgaaatggct gtttaaaagc catccgcctt tgctgtttcg ccccgacggc agcctgtatc
    16861 aaatcgaatg gctgtggcaa atgctgcaaa actgcacggc agcgcactat caaaccaata
    16921 aagagcgcat ggtcaggatt tccgaatata gccgtgaaat gttccgccgt tttgaagcgc
    16981 aaaccggcat gaattttgag gaacgcaaaa aagggacgtt gcagattttc cgccaaacca
    17041 aagaagtcga agcggcagaa caagacattg ccgttttgga acgctacggc gtgccgtacc
    17101 gccgtctgaa acccgaagaa tgtgcagaat ttgagcctgc gctggcacgc gttaccgcca
    17161 aaattgccgg cggcctgcac ctgcctgcag atgcgaccgg cgactgccac ctcttcaccg
    17221 aaaacctata caaattgtgt caggaaaagg gcgtacagtt tcatttcaac caaaccatca
    17281 gccgcatcga ccataacggg ctgcgcatca aagccgttga aaccgaaaca gggcggtttg
    17341 aagcagatgc cgttgtctgt gcgctcggct gcttcagtag gactgtgttg gcgcagttgg
    17401 atctcaatct gccgatttat cccgtcaaag gctattcctt gaccctgccg gtcaccaatt
    17461 cagacggcgc gccggtgtcc accgttttgg atgaaagcta caaagtcgct atcacgcgct
    17521 ttgacaacag aatccgcgtc ggcggcatgg cagaattgtc gggctacgaa atcaaactgc
    17581 ccgaaaaacg ccgcgaaacc ttggctttgg tcgtcaacga tttgttcccg gaaggcggcg
    17641 atttgagcca agcattgttc tggagcggcc tcagaccgat gacgcccgac agcacgccgt
    17701 taatcggccg cacccgcttt gaaaacttat tcttgaatac aggccacggc actttgggct
    17761 ggaccatgtc gctgggttcg gcaaaattaa ccgccgatat cgtgagcggc aaagacaccg
    17821 aaatccgcag cgacgatttg agcctgtcgc gttatcaggc ttaagctgga aagcactaag
    17881 gtttagcggc atttcaaatt taaaggccgt ctgaaaatca tttttcagac ggtcttttgt
    17941 ttcaaaatcc gtgtatccaa tcaataaaat aaataataac tattatcata tacattaaaa
    18001 agtgttagtc tgaacctgtc ccagctttta gcgcaggttt tctcatgccc gatttttcga
    18061 tgtccaattt ggccgttgcc ttttccatca cattggctgc cggtttgttt accgtattag
    18121 gcagtggctt ggtgatgttt tccaaaacgc ccaatccgcg tgtgttgtcg tttggtttgg
    18181 cgtttgccgg cggtgcgatg gtatatgttt ccctgacgga gattttcagt aaatccagcg
    18241 aggcgttcgc tgaaatttat gataaagacc acgcgtttgc ggcggcgacc atggcatttt
    18301 tggccggtat gggcggcatt gcgctgattg accgcctggt gccgaaccca catgaaactt
    18361 tagacgcgca agacccgtcg tttcaagaaa gtaaacgccg ccatattgcg cgagtcggca
    18421 tgatggcggc gtttgcgatt acggcgcaca actttcccga aggtttggcg acgttttttg
    18481 ccaccttgga aaatccggcg gtcgggatgc ctttggcctt ggcgattgcc atccataata
    18541 ttccggaggg catttccatc gccgcgccgg tttattttgc cacccgcagc cgtaagaaaa
    18601 cggtatgggc gtgtctgcta tccggcttgg ccgagccgtt gggggcggct ttgggctatt
    18661 tggttttgca gccgtttttg tcgcctgccg tgtttggttc ggtattcggc gtgatagccg
    18721 gtgtgatggt gtttttggcg ttggacgagc tgctgccggc tgccaaacgc tattcagacg
    18781 gccatgaaac cgtttacggc ctgacaatgg gcatggcggt gattgccgtc agcctggtat
    18841 tgttccattt ttaaaacctg tatgccaaag gccgtctgaa atgattcaga cggccttttt
    18901 gcgttataat ccgcacatca aaaataacct taatttcaca aagttccatc atgttagaca
    18961 aatacaatcc agccgaaatc gaatccaaac attatcaaaa ctgggaagag cagggctatt
    19021 tccagcctga tatggatttg accaaaccgt ctttctccat ccaactgccg ccgcccaacg
    19081 taaccggcac gctgcacatg ggtcatgcct tcaaccaaac cattatggac ggcctgaccc
    19141 gctactaccg catgaaaggc tgcaacaccg cttggattcc cggcaccgac cacgcaggca
    19201 tcgccacgca aatcgtggtc gagcgtcagc ttgccgcgca aaacgtgtcc cgtcatgact
    19261 tggggcgcga aaaattcttg gaaaaagtgt gggaatggaa agaagtttcc ggcggcacga
    19321 ttacccagca gatgcgccgc gtgggctgct ctgccgactg gacgcgcgag tatttcacga
    19381 tggacgacgt gcgcgccgaa accgtgaccg aagtgttcgt gcgcctgtat gagcaaggct
    19441 tgatttaccg cggcaaacgc ttggtgaact gggatccggt attgggtacg gcagtatcgg
    19501 atttggaagt ggaaagcgtg gaagaacaag gctctatgtg gcacatccgc tatccgctgg
    19561 cggacaatcc tgccgaagcc gtgattgtgg cgaccacccg ccccgaaacg ctgctgggcg
    19621 acgttgccgt tgccgtcaat cccgaagacg aacgttatac ccatttgatt ggcaaggaat
    19681 taatcctgcc gctgaccggc cgcaccattc ccgtgattgc cgacgaatac gtcgaaaaag
    19741 acttcggcac aggctgcgta aaaatcacgc ccgcgcacga cttcaacgac tacgaagtcg
    19801 gcaaacgcca cgacacgcgc ctgattaatg tgttcgactt ggaagccaaa gtgctggcaa
    19861 acgccgaagt gttcaacttc aaaggcgaag cgcaaccagg ctttgccctg cctgaaaaat
    19921 acgcaggctt ggaccgcttt gccgcgcgca aacaaatggt tgccgatttg caggaacaag
    19981 gcttgttggt cgaaatcaaa gcgcacacgc tgatgacgcc gaaaggcgac cgaacaggct
    20041 cagtgattga gccgatgctg accagccaat ggtttgtcgc catgtccgcc acaccgaacg
    20101 gcggcgagcc tgacagcgaa ttcaaaggct tgagcctcgc cgacaaagcc aaaaaagccg
    20161 tcgacagcgg cacggtacgc tttatccccg aaaactgggt caacacttac aaccaatgga
    20221 tgaacaacat ccaagactgg tgtatctcgc gccaactgtg gtggggccat caaattcctg
    20281 cgtggtatga cgaagcaggc aatgtttacg tcgcccgcaa tcaggcggaa gccgaaaaac
    20341 aagccggcaa aaccggtttg acccgcgaag aagacgtatt agacacatgg ttctcctccg
    20401 cgctcgtgcc gttctccacg ctcggctggc cgtctgaaac cgacgaactc aaagccttcc
    20461 tgccgtccaa cgtcttggta acaggctacg aaatcatctt cttctgggtg gcgcgtatga
    20521 ttatgatgac cacccacttc accggcaaag taccgtttaa agacgtgtac atccacggca
    20581 tcgtgcgcga ccacgaaggc aaaaaaatgt ccaaatccga aggcaacgtc atcgaccccg
    20641 tcgatttgat tgacggtatc gacttggaca aactgctggt gaaacgtacc accggcctgc
    20701 gcaaacccga aactgcgccg aaagtggaag aagccacgaa aaaactcttc cccgaaggca
    20761 tccccagcat gggtgcggac gctttgcgct tcactatggc gagctacgcc agcttggggc
    20821 gttcggtcaa cttcgacttc aaacgcgccg aaggctaccg caacttctgc aacaaaatct
    20881 ggaacgccac caacttcgtc ttgatgaaca ccgaaaacca agactgcggc tacggcgcaa
    20941 ccgctgccga accgcgcggc tattccttcc ccgatatgtg gattgtaggc cgtctgaatc
    21001 agaccatcga gcaggttacg caagcctacg aaacctaccg ctttgatttg gcggcagaaa
    21061 ccctgtacag cttcgtatgg aacgactatt gcgactggta tttggaactt gccaaagtgc
    21121 agcttcaaac cggctgcgcc agccgccaac gcgccacacg ccataccttg ttgcgcgtac
    21181 tcgaagccgc cctgcgcctg ctgcacccga ttatcccgtt catcaccgaa gaattgtggc
    21241 aaaccgtcgc tcctatgtgc gacgcgaaaa ccgccgacag catcatgctc gcccgcttcc
    21301 ccgaagccga cggcggcgat attgttcaga cggcattcga gcaaatgacc gtgttgcagg
    21361 atttgattgg cgcagtccgc aacctgcgcg gcgaaatggg catccagccc aatgtgaaag
    21421 ccccgctgtt tgtcgaaagc gcagacgact tggcggacta cctcaaatac ctgccgatga
    21481 tgacccgcct gatcgaagca cagcaagtcg ccgccctacc cgaaagcgaa gacgcgcccg
    21541 tcgccgtctg caacggcgcg cgcctgatgt tgaaagtcga aatcgacaaa gctgcggaaa
    21601 ccgcccgttt gagcaaagaa gccgagaagc tgcaaaaagc cttggacaaa ctcaacgcca
    21661 aactctccaa acccggctac accgaaaaag ccccagcgca tttggtggaa aaagacaaag
    21721 ccgatttggc ggaattggaa gacaaaatgg caaaagtgca aactcagttg tcgaagttga
    21781 aagactgatt tgattgaatc aacaaggccg tctgaaaaat gt
//
LOCUS       DAWKST010000012        21751 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.012, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000012 DAWKST010000000
VERSION     DAWKST010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21751
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.012"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..808
                     /locus_tag="WJL87_000316"
     CDS             <1..808
                     /locus_tag="WJL87_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7798803.1"
                     /translation="RYRTPNIKYIYQDNQGTAEARNTGIKNSNGKYIVFIDSDDFINC
                     QVLLDFLSKDDIDMPDVVFLNAVKYDKGSVSHFGEDYQPEKILNQSKVEVLKGLCRFR
                     KFPGSACNKIIKRELIIKEKLFFEKEVYAEDIEWSMRLFNAATTFSYLDGCYYYYRQG
                     RKDSKTGTVSEKSIKSLLYILEKNAEMEFNRDISSYLYSFLSYEYLVLLFIMTSKNIE
                     CDADIKRRAYHLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKAIRGNI"
     gene            849..1379
                     /locus_tag="WJL87_000317"
     CDS             849..1379
                     /locus_tag="WJL87_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ7798804.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGTTVLDDAVIEDEVMIGAGSLVPPRKRLAGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(1390..1529)
                     /locus_tag="WJL87_000318"
                     /pseudo
     CDS             complement(1390..1529)
                     /locus_tag="WJL87_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor"
     gene            complement(1548..3143)
                     /locus_tag="WJL87_000319"
     CDS             complement(1548..3143)
                     /locus_tag="WJL87_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ7798805.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATVRMVEPDEAKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(3250..3645)
                     /gene="hisI"
                     /locus_tag="WJL87_000320"
     CDS             complement(3250..3645)
                     /gene="hisI"
                     /locus_tag="WJL87_000320"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ7798806.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            complement(3676..4443)
                     /gene="hisF"
                     /locus_tag="WJL87_000321"
     CDS             complement(3676..4443)
                     /gene="hisF"
                     /locus_tag="WJL87_000321"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ7798807.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNSENTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(4456..5193)
                     /gene="hisA"
                     /locus_tag="WJL87_000322"
     CDS             complement(4456..5193)
                     /gene="hisA"
                     /locus_tag="WJL87_000322"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ7798808.1"
                     /translation="MLLIPAIDLKEGRCVRLRQGLMEEATVFSDSPAETALHWFEQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(5226..5864)
                     /gene="hisH"
                     /locus_tag="WJL87_000323"
     CDS             complement(5226..5864)
                     /gene="hisH"
                     /locus_tag="WJL87_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ7798809.1"
                     /translation="MQIAIIDYGMGNLHSVLKSVWTAGQLAGKNTEIFLSGDPDRVSR
                     ADKVIFPGQGAMPDCIAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDPQGCRLKVPHMGWNTVRQTQNHPLFQGIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(5995..7497)
                     /gene="pta"
                     /locus_tag="WJL87_000324"
     CDS             complement(5995..7497)
                     /gene="pta"
                     /locus_tag="WJL87_000324"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ7798810.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVGPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(7682..8740)
                     /gene="fbpC"
                     /locus_tag="WJL87_000325"
     CDS             complement(7682..8740)
                     /gene="fbpC"
                     /locus_tag="WJL87_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ7798811.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(8761..10278)
                     /gene="fbpB"
                     /locus_tag="WJL87_000326"
     CDS             complement(8761..10278)
                     /gene="fbpB"
                     /locus_tag="WJL87_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ7798812.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINLAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(10346..11341)
                     /gene="fbpA"
                     /locus_tag="WJL87_000327"
     CDS             complement(10346..11341)
                     /gene="fbpA"
                     /locus_tag="WJL87_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ7798813.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(11592..12062)
                     /locus_tag="WJL87_000328"
     CDS             complement(11592..12062)
                     /locus_tag="WJL87_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HEZ7798814.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFERHDGKLESMDGWEQELVLIW
                     RAEADIGNGGILQFVCNWGFPAAEKTCSVLKKIGAVHSAMLIHRAADALRKEIRHLQS
                     EGKNLKEMWDITKHLDNKTASLPNSLDEQYWQDPDKLYLPGWQYYSGNPVQTLF"
     gene            complement(12120..13496)
                     /gene="argH"
                     /locus_tag="WJL87_000329"
     CDS             complement(12120..13496)
                     /gene="argH"
                     /locus_tag="WJL87_000329"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ7798815.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIRSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRCRVNRMPLGAAALAGTTYPIQREITAELLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADEAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMLA"
     gene            complement(13515..14384)
                     /gene="galU"
                     /locus_tag="WJL87_000330"
     CDS             complement(13515..14384)
                     /gene="galU"
                     /locus_tag="WJL87_000330"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ7798816.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRIIGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(14412..15011)
                     /gene="rdgB"
                     /locus_tag="WJL87_000331"
     CDS             complement(14412..15011)
                     /gene="rdgB"
                     /locus_tag="WJL87_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ7798817.1"
                     /translation="MSEKPEKIVLASGNAGKLKEFGNLFEPYGITVLPQSEFSIPECP
                     EPYSTFVENALAKARHAAGHSGLPALADDSGICAAALNGAPGIHSARYAGSNPKSDTA
                     NNLKLAAELAGKADKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHRAQALAELLRKLAL"
     gene            complement(15004..15237)
                     /locus_tag="WJL87_000332"
     CDS             complement(15004..15237)
                     /locus_tag="WJL87_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798818.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGSLALYLRHKSLRAAQSAAKENRYV"
     gene            complement(15345..15878)
                     /locus_tag="WJL87_000333"
     CDS             complement(15345..15878)
                     /locus_tag="WJL87_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ7798819.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(15979..16437)
                     /gene="nudB"
                     /locus_tag="WJL87_000334"
     CDS             complement(15979..16437)
                     /gene="nudB"
                     /locus_tag="WJL87_000334"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ7798820.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPEGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPIALQPEEHVSYGWFDMEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            16648..16724
                     /locus_tag="WJL87_000335"
     tRNA            16648..16724
                     /locus_tag="WJL87_000335"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16682..16684,aa:Pro,seq:ggg)
     gene            16882..18708
                     /gene="mafB"
                     /locus_tag="WJL87_000336"
     CDS             16882..18708
                     /gene="mafB"
                     /locus_tag="WJL87_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HEZ7798821.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFMAAIPIKGIGAVRGKYGLGGI
                     TAHPIKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQEIRNGNKNSNFSQHAQLEREINKLKSADEI
                     NFADGMGKFTDSMNDKAFSRLVKSVKENGFTNPVVEYVEINGKAYIVRGNNRVFAAEY
                     LGRIHELKFKKVDFPVPNTSWKNPTDVLNESGNVKRPRYRSK"
     gene            18711..19175
                     /locus_tag="WJL87_000337"
     CDS             18711..19175
                     /locus_tag="WJL87_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798822.1"
                     /translation="MAIWNYRYYLIPSAAIRNKFNANKDIILDEYRSNGFQNFNENKS
                     FENYFIDNDVILLSIINEAKKQLKLKESWDKDAIMFCDNFGNSLTVWPDDIECELDLR
                     FDYTKFIQKTIDWAIKYNCLLVIEKTGNVVSPNINNLMYEIKAYLESKPWPI"
     gene            19364..19546
                     /locus_tag="WJL87_000338"
                     /pseudo
     CDS             19364..19546
                     /locus_tag="WJL87_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223961.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
     gene            19558..19845
                     /locus_tag="WJL87_000339"
     CDS             19558..19845
                     /locus_tag="WJL87_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEZ7798823.1"
                     /translation="MQLEIIGSKIYTEQDFHNQISKIFSIQDYYGNNLDALWDLLSTN
                     VERPITLVWKDAMFSKNQLENIFIEIVNVLERVKKQDEDYGFEEKFNYILE"
     gene            20341..21603
                     /locus_tag="WJL87_000340"
     CDS             20341..21603
                     /locus_tag="WJL87_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ7798824.1"
                     /translation="MKSKSGVIASLTGAAVGGTPVDAQTGGAVGQNAVENNLYLTSEA
                     LKKDKQTARKIYSVIKEQVKHECSSTGRITECRQNIGRIIEFTQDKRFDSRFKDLKKE
                     SLYYLNKHPDLVASYLKAEYEKLDREDKSILHRYISPGAEIVSGSLGVVLSGVAGGGS
                     CAETFGLGCAAALVGVTSSYDHVITGTKNFGKKASEQRPTIAVQALKQLGLSEQAAEY
                     VQFSIDLFSVGKSGGGIPKAKPVFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQS
                     SQFKAHAQREWENKTGLDFNHFIGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGV
                     YQATVEIKKPDGSWEVKTKKGGKVMTKHTMFPKDWDEARIRAEVTSAWEKRKELEGQK
                     WRGTSKSGVEIEGFTEPNRTAYPLYNSK"
BASE COUNT         5073 a         5026 c         6275 g         5377 t
ORIGIN      
        1 cagataccga acaccaaaca tcaaatatat atatcaggat aaccaaggga cggcagaagc
       61 taggaatacc ggaataaaaa attcaaacgg aaaatatata gtctttattg attcagatga
      121 ttttattaat tgtcaggtct tgctggattt tcttagtaaa gatgatattg atatgccgga
      181 tgtggttttt ttaaatgcgg ttaaatatga taagggaagt gtttcacatt ttggcgaaga
      241 ttatcagcct gaaaaaatac tcaatcaatc caaagtcgaa gttttgaaag gattatgccg
      301 atttagaaaa tttccgggtt cggcgtgtaa taagattata aaaagggaat tgattatcaa
      361 agaaaaactg ttttttgaaa aagaggttta tgccgaagat atcgaatggt caatgagatt
      421 atttaatgcg gcaacaactt tttcttattt ggacggttgt tattactatt atcggcaggg
      481 aagaaaagat tctaaaacag gaactgtttc ggaaaaaagt ataaagtcat tattatatat
      541 tttggagaaa aatgcggaaa tggaatttaa tagggatata tcaagttatc tttattcttt
      601 tctttcctac gaatatctcg ttttgctttt tatcatgacg agtaaaaata tagagtgtga
      661 tgctgatata aaaagaaggg cgtatcattt aaggtttatg ctgttaaagt ccaataaatt
      721 gatatataag ctgatattcc cgataatcac attattcggg gttgatatta caggcaggat
      781 tttaaaagca atcaggggga atatttaata aatcctttaa cactatacat cttaccgaag
      841 gaggaaaaat gaacgcaatc cgaactttcc aaaaccgcac gcccgaaatc cacgaaagct
      901 gtatgataga cgaagcgtgc gtcgtcatcg gcgaagtgtc gcttgccgaa gacgtttccg
      961 tgtggccgtg cgccgtgttg cgcggcgatg tgaacagcat caccgtcggc gcgcgcagca
     1021 atatacagga cggcagcgtc ctgcacgttt cccacaaaac cgccgccaaa cccgaagggt
     1081 cgccgctggt tatcggcgaa gacgttaccg tggggcacaa agtgatgctg cacggctgcc
     1141 gtatcggcaa ccgcgtcctg gtcggcatgg ggacgacggt tctggacgat gccgtgattg
     1201 aggacgaagt gatgatcggc gcgggcagcc tcgtgcctcc gcgcaaacgt ttggcgggcg
     1261 gctaccttta tgtcggttcg ccggtcagac aagtgcgcgt gctgaccgat gaggaaaaag
     1321 cctttttgaa atattccgcc gcgcattatg tgaagctgtc gaaacagtac gggatgtgaa
     1381 atcacatcgg cgttcttgcg tcagccccaa attcatgcgg atgggacgca tccgataacg
     1441 gtatccgatg cgccttgatt ttgaccgtct gcgtttgagt tgcaggcaaa aatgccgtct
     1501 gaagcccttt tttcgggttc agacggcatt ttattgccga ttgcttttta aagtttgacc
     1561 gaatgctcgc gcgtttcgtg gaacacgatg tccggccagc gttcttgcgt gagtcccaga
     1621 ttcacgcggt taggggcgag gtaggcgagg ttgccgcccg cgtcgatggc gaggttgcct
     1681 gcattggctt tttcaaattc cgccagtttt ttcttgtcgt cgcacgatac ccagcgcgcc
     1741 gaccagatgg atgcgctgtc gaacacggct tctacgccgt attcgttggc cagccgcgag
     1801 gtaacgactt caaattgcaa cacgccgacc gcgcccaaaa tcaaatccgc gccgctcatc
     1861 ggtttgaaca cctgcaccgc accttcttcg ccaagctgtt gcaaaccttt ttgcagttgc
     1921 ttgattttca gcgggttttt gatgcgaacg ctgcggaaca gttcgggcgc gaagaaggga
     1981 atgcctgtaa atgccagttg ttcgccttcg gagaagctgt cgccgatttg gatgttgccg
     2041 tggttcggga tgccgatgat gtcgccggcg taggcttctt ccaccagctc gcggtcgtgc
     2101 gacatgaagg ttaccacgct ggaggcggcg atttcgcggt tgatgcgcag gtgtttcatc
     2161 ttcatgccgc gctcgaattt gccggagcag acgcgcaaaa aagcgatgcg gtcgcggtgt
     2221 ttcggatcca tattggcttg gattttaaag ataaatccgg aaaacttagc ctcgtccggt
     2281 tcgaccatac gcacggtcgc gtcgcgcggt ttcggcgcgg gcgcccagtc aatcaatgaa
     2341 ttgaggattt cctgaatacc gaagttgtta atcgccgaac cgaaaaacac gggcgtgagt
     2401 tcgccggcga gaaattcgtc gagattaaac tcgttcgatg ctgcctgcac taattcgatt
     2461 tcgtcgcgca actgttggat ttccaacgga aagcgttgtt ccaattcagg attatcgata
     2521 cctttgatga tgtcgaattc gtgcggcaga cgttcgccgc cggcttcaaa gagatagatt
     2581 tcatcgttca ggatgtggta tacgcctttg aagtttttgc ccataccaat cggccaggta
     2641 acgggcgcgc agcggatttg caggatgttt tccacttcgt ccaagagttc cagcgagtcg
     2701 cgcacttcgc ggtcgtattt gttcataaac gtaacaatcg gcgtatcgcg caggcggcag
     2761 acgtttaaga gcttgatggt ttgcgcttcc acaccttttg ccgcgtcaat caccattaat
     2821 gcgctgtcca cggcggttaa aacgcggtag gtgtcttcgg agaagtcttg gtgtcccggc
     2881 gtgtccaaga ggttgacggt gtggtcttta tagtcgaact gcatcacgct tgatgccacg
     2941 gaaatgccgc gctgcttctc gatgtccatc cagtcggagg tggcgaattt gccggttttc
     3001 ttgcctttta ccgtacccgc gctttgaatc gcacctgaaa acagcaagag tttttcagtc
     3061 aacgtggttt tacctgcgtc agggtgggag atgatggcaa acgtgcggcg gcggcgcact
     3121 tggtcgagga tttcttggga catggttttc tttgcaaaaa ggttcaggcc gcttttcaga
     3181 cggcccggac agtgtttgag acggcgaaat tgtacaaaaa aatgcctgat aattcaatgt
     3241 tggaggcggt cagtgcgtgc tgccgtaaat ctctttttcg tctttcagga cggcatcggc
     3301 ggtttcccac gcgctgccac gccagacttt gtaaaagcag ctttctcgcc cggtgtggca
     3361 ggcgatgccg ccgttttggg cgatgagcat cacaatggcg tcgccgtcgc agtcgaggcg
     3421 cagttcgcgg actttttgcg tgtgtcccga ctcttcgccc ttcatccatt gtttttggcg
     3481 cgaacggctg taatagtggg caaagccggt ttcgacggtt ttttgcaggg cttcggcgtt
     3541 catccacgcc accattaaaa cacgtttggt ttcggcatct tgggcgatgg cgcaaaccaa
     3601 acctttttcg tcaaatttga cggcttcaag caggttttta tccatatttc ctttcagacg
     3661 gcatagtcgg agcggtcaga ggcgcacttc gatgccggct tcgcgcatag cgcgtttggc
     3721 ttcgcggatg gcgatttccc cgaaatggaa aatgccggcg gcaagtacgg catcggcttt
     3781 gccttcggtt ataccttcaa tcaggtgccg gacattgccg acccctccgg aggcgatgac
     3841 ggggatgtcg acggcttcgg caacggcgcg ggtcagcggc aggttgaaac cctgtttcgt
     3901 accgtccctg tccataccgg taagcaggat ttcgcccgcg ccgcgttttt gcatttcgat
     3961 cgcccattcc accgcatcca aaccggtcgg attgcgcccg ccgtgggtaa agatttccca
     4021 gcgtgtgttt tcggagttga cggctttggc atcgacggcg gcgacgatgg cttgcgaacc
     4081 gaaaaatccg gcggcttcgt caatcaggtc gggacgggta acggcggcgg tgttgatgct
     4141 gactttgtcc gcgccggcat tgagcaggcg gcggatgtcg gcaacggtgc gtacgccgcc
     4201 gccgacggtc agcgggatga agacttgtcc ggcaacttct tcgatgatgt gcaggatggt
     4261 gtcgcggttg tcggatgagg cggtgatgtc gaggaaggtc aattcgtccg cgccttcgcc
     4321 gttgtagcgt ttggcggctt cgacggggtc gcccgcgtcg cgcaaaccga tgaagttcac
     4381 gcctttgacg acgcgcccgt cttttacgtc gagacagggg atgatgcgtt ttgccagtgc
     4441 cataatcgga tgcctttagt cgagggaatc tgccagttgc tgcgcttggg caaaatcgat
     4501 gctgccctcg taaatcgcgc ggccggtaat cgcgcctgct acgccatgtt tttcggcggc
     4561 acacagggcg cggatgtcgt ccaagccggt cagtccgccg gaggcgatga cgggaatgcg
     4621 gacggcttgg gcgagtttga ccgtcgcgtc gatgttcacg ccgctcatca taccgtcgcg
     4681 cccgatgtcg gtgtagatga tgctgttgac gccgtcgtct tcaaagcgtt ttgccaaatc
     4741 aattacatga tgcccggtta cggttgccca gccgtcgatg gcggccatac cgtctttggc
     4801 atccagcccg acaataatcc tgccggggaa ggctttgcac gcctcgcgca cgaagtcggg
     4861 gtttttgacc gccgccgtgc cgataatcac gtcgtttaag cccaaatcca aatattgtcc
     4921 gatggttttc aaatcgcgta tgccgccgcc gagctgtacg gggatgtctt tggcgacggc
     4981 ggcaaggatg tctttgatgg cgggcaggtt ttgcggaacg ccggcaaacg cgccgttcaa
     5041 atctaccaga tgcaggcggc gcgcgccttg ttcgaaccag tgcagcgcgg tttcggcggg
     5101 cgaatcggaa aagacggtcg cctcttccat cagcccttgt ctcaggcgga cgcagcgtcc
     5161 ttctttcaaa tcgatggcgg gtatcagcag catagttttt ctccttgtgc ggggccgtgt
     5221 ccggcttacc agtttaaaaa gtttttcaac atcgtcagcc cggcatcgtg gcttttttcg
     5281 gtgtgaaatt gcgtggcgaa tacgttgtct ttgccgacga tgcaggcaaa cggggacggg
     5341 tagtcgcttt cgcccaatat ggtttcggga ttttcggggg cgaaatagta gctgtggacg
     5401 aagtaaaaac gcgtgtcttg ggggatgcct tgaaaaagcg ggtggttttg ggtttggcgc
     5461 acggtgttcc agcccatatg cgggactttc agacggcatc cctgcgggtc gcggaggtcg
     5521 cgctcaaagc gtctgacttt gccgccgaac cagcccaagc cgtcggtgtt tccttcttcg
     5581 ctgtggtcga ataaaagttg cgcgccgacg cagattccga aaaagggttt gttttttaag
     5641 gcatctttga ctgcctcgtc caagccgccg cgcgtcagtg ccgcaataca gtcgggcatc
     5701 gcgccctgac cgggaaaaat gactttgtcg gcgcgggaca cgcggtcggg gtcgccgctt
     5761 aaaaagattt cggtattttt tccggcaagc tgccccgccg tccagacgga tttcaatacg
     5821 gaatgcaggt tgcccatacc gtaatcgata atggcgattt gcatggcttc ctcctctttt
     5881 tttgcaatat ggctgcgatt ttaacaaaca aatgtgccgt gctgataaaa atgccgtctg
     5941 aaaacgggag tctgtcttca gacggcatag ggtttaaacc cggaaagccg tttgtcagcc
     6001 ttccatttgt tttgcctgaa cggcagttag ggcgatggtg aacacgatgt cttccaccag
     6061 cgcgccgcgc gacaggtcgt tgaccggttt gcgcaggcct tgcagcagcg ggccgacgct
     6121 taagacgttg gcactgcgtt ggacggcttt ataggtgcag ttgccggtgt tcaggtcggg
     6181 gaagaccaaa acggttgcct gtcctgccac cgggctgccc ggagctttgg atttgcccac
     6241 gcccggcacg gttgccgcat catattgcag cgggccgtcg atggcgaggt cggggcgttt
     6301 ttcccgggca agtttggttg cttcgatgac ggtatcgaca tcggggccgc tgccggagtt
     6361 gacggtggag taggaaatca tcgccacttt cgggtcgatg ccgaaggctt ttgcggaatc
     6421 ggcagactgg atggcgatgt cggcaagctg ttgcgcggtc gggttcggat taaccgcgca
     6481 gtcgccgaag acgaggactt ggttgggcag cagcataaag aatacgctgg acacgaggct
     6541 tgcgcccggt gcggttttaa tcagttgcaa agcggggcgg atggtgttgg cggtggtgtg
     6601 aaccgcaccg gataccaaac cgtccacatc attttgcgcc atcatcatcg taccgagtac
     6661 cacggtgtct tgcagttgct tgcgcgcgtc ttcgggtgtc aggcctttgg atttgcgcag
     6721 ttcgcacatc ggtccgacgt attgttcgac caatgaggcg ggatcgatga tttccaaaga
     6781 gtcgggcagg ctgatgccgc gttctttggc aacggcttcg acttcttcgc gtttggcaag
     6841 caggacgcag cgggcaatgc ctttttcgtg gcagatggcg gcggcttgga cggtgcgggg
     6901 ttctgcgccc tcaggcagaa cgatgcgttt gtcggcttgg cgggcgaagt cgatcaggtt
     6961 gtagcggaat tgcgccggcg acaggcgttt tgcttcgcgg cctgccaata cggatacgtc
     7021 tttcagcgcg tcgctcgaac cgaagaaggt caggccggtt ttttcggctg ccgcttcggc
     7081 aacggaggct gccgcgccgt ccacgacaaa accttccaat acgcccggcg cggcggcgaa
     7141 gaactgtttg gcaaggttca gccgatttgc cagttcgtcg gcatcggcgt tgtcggaacg
     7201 gacggcgaag acggctgccg cgtcaaggga caatgccagt tcgacgtttt tgcctgcgag
     7261 gtagattttg tcggcatcgg gcgcgatgcc ttcgatgacg aggttggcgg catcgagtgc
     7321 ggcaactttg ccgaccagtg cgtcgaacca gtcgtcgctt ttgccttgcg cgagcagggt
     7381 ttcggcggtt gcgtcaacgg cttggaaaat ttgtgcgtcc agtgcttttg caaaggcttg
     7441 tgcggcggcg gaggcgtcca gtccggcaga tacgggtacg atgagtactt ttgccatgat
     7501 atatcctttc gtatgctgcg gtgtgcggca tatgtggttg gaaggggcgg catataggca
     7561 gaaacggctg cctgcgtgcc gtgcgtgccg tgtttggctt gaggcgcgca ggttgaatat
     7621 agcaaacaaa ttctgtttcc aacaagataa atatccgcag gcttgtggat gctgccgcct
     7681 ttcagagggt atttccgggg aagaacaggg cgggaccgtc caaatggagg acggcggaaa
     7741 tgccgtctga cagggtgggg gcggaaggga ggttgagcgt gaggacggtt tgtccgaccc
     7801 ggaggctgat ttcggtatgc cgcgctttgg gcgtggtttt gagaaccacg gcgtgaatgg
     7861 aggcggtggg tgcggaatgg gggtgaaggc tgaactgttc cggacggatg agcagtgtgc
     7921 cgcgtgtgcc tgcgggtgcg ccgctttgaa cgggcaggcg gcccaatccg caatcggcgg
     7981 tgccgtcggc gttgagcgcg gcggggaaca cgatgccttc gccgataaac agggcggcat
     8041 caaggtcggc aggttgtcgg tacaattcgt gagggcttgc agtttggagg atgcgcccct
     8101 gtttcatcac ggcaatccgg tcggcgtatt gcagggcttc ttcgcggtcg tggctgacga
     8161 aaacggcaga tttgccgttg gcgcgcaggg cggcaatcat gtcttcgcga atctggcggc
     8221 gcaactgttc gtccagcgcg ctgaagggtt cgtccaacaa aatcagttcg ggatcgggtg
     8281 cgagggcgcg ggcgagggcg acgcgctgtt gctgtccgcc cgaaagttcg tgcggatagc
     8341 gtccggcaag ttcggaaatg ccggtcaatt ccaacatagc ttcgatgcgc tgccgctctt
     8401 gcgccgtctt gcctttgccg ttgcccagcc cgtaggcggt gttgcggtaa acggtcaggt
     8461 gggggaacag cacaccttcc tgtacgacat aacccaaacg gcgttcgcgg acggggaggt
     8521 tggtattttt cgagaagatg gttctgccgg aaagcgaaat ttcgccaaaa tcgggttgtt
     8581 caaaaccggc aaggcagcgt aaaagggtgg ttttgccgca gccggacgcg ccgacgataa
     8641 agaggatttc gcccgggtcg aggctgagcg aaatgtcgtt taaaactggg gtgttttgaa
     8701 aacttttgga caggtgtccg atgtgcaggg cggcggtcat ggcggtactt cctcaagctg
     8761 ttatttgaag gcgtatttct tcagcaggaa tacggggatg ccggaaaata ataccagcat
     8821 cagcgcgtaa ggggtggcgg cggcgtattg tgcgtccgat gtgtattccc aaacggcggt
     8881 ggagagtgtg tggacatcgt cggtggtcag cagcagggtg gcggtcagct ctttcatcag
     8941 tttgaggaag acgagtgcga atgcggcggt aatgccgggc aggatggacg gcagtaccaa
     9001 cgtcctgaaa ataaagaagt gtccgcgccc caatgttgcg ccgacctgtt ccatcccttt
     9061 tgggagttgt tccaaggaag tcctcagggt ggtttgcgcc atcggcaggt aaagcatgaa
     9121 ataggcaagg atgacgacga taaaggtttg gtaaacggca ggggtgtagt tgatgctgaa
     9181 ataaaccaag gatagggcga taaccaaacc ggggacggcg tgcagtaaaa acggcagcct
     9241 gtctatccaa acggttaaaa aattgcgata gcgaaccgat gcccaaacaa ggggcaaggc
     9301 acataatata gtcaaaatcg cacctaaagc cgatacgctt aaggaacgga taaaggcatc
     9361 aaatacggat acgagcgcga atgtgccgga agtgccgacc atcatccaat gtatcaatac
     9421 gccaaagggg ataataatgc ccaaagtcaa caagctgctt aaaaaaacaa tcgcgccgat
     9481 ctgaccgggc agtttgaggg ttttgacggg ataaggacgg gcaacgcctt tgccgctgtg
     9541 gtaaatcttg gctttgccgc gaaatatgct ttctccaaat acgacgatgc cgcacaccgc
     9601 cattaaaaca gcggaaagca gggcggcggt attgttgttg taggacattt cgtattcttg
     9661 gaaaatggcg gtggtaaaag tggggtagtt caaaatagat accgcgccaa attcgaccag
     9721 catatgcagg gcaatcagta acacgctgct gccgatggcg ggtttgagct gggggaggat
     9781 ggcggaaaaa aaggtttgca ggcggctttt gcccaaggac aggctgactt cttcgtaaga
     9841 caggctgatg cgtttgagtg ccgcctcgac gggcaggtag gcgagcggga acgaggacag
     9901 gctcataatc atcactgtcc cccaaaagcc ttcgacacgg aaggtcaggc tgatccaggt
     9961 gaaacagctg acaaatgcgg ggatgcacaa aggcagggtg attgccgtct gaaaaaaggt
    10021 tttgccgaag aagcggtaac gttggaacaa aagggcgcag gcaatgccca aaacaatgga
    10081 aatcagggta acgcccgcca tcatcgtcaa ggtgttggag agcaaatccc acatacgcgg
    10141 gcggaacaac agttcgacgg cgaggttgat gccgacctgc cacgaacgca tagcgacata
    10201 taaaaaaggc agggtaagcg gtagggcaat cagtaggatg aggccggtaa gccaaatggg
    10261 tattttttta ggagacatag tgttttttat cggcaaaacg ggcggacagt ataaatgtcc
    10321 acccgtttga caatccgaaa acggcttatt tcataccggc ttgctcaagc agccgggtgg
    10381 cgtgttcttt ttcggaaaca gtggtggcgg acacttgggg tgcttccaac ttggcgatgg
    10441 gttccaaatt gaaagtggat accacgtgcg gattcaaagg atattcggca cggacggcgg
    10501 tcagggcgcg ctgtccttcc ttgctggcga ggaaggcgac gaattttttc gcctcatcct
    10561 tgttttggga ggattttaac acggctgcgc cggaataggt aacgagtgcg ccgggatctc
    10621 tgtggcggac gaaattcagg cgggtgtgga cattttgtac gcctttttca cgcgcaaaag
    10681 cgtgccagta gtagttgttg atgagggcgg catcgatttc gccgttttca accgcttgaa
    10741 gggcgacgga gtttttagcg taaggcttgc cgtattcttt cagacctttg agccatttca
    10801 atgcggccgc ttcgcctttc agtttgacga tggcgacaac ctgttccaag aacgcgccgg
    10861 aagtgggggc gtaaccgatg cggtttttcc atttcggcgt ggcgtaattc aggacggatt
    10921 tttccaaatc tttttcagac agtttgcggg tgtcgtaaac gacgacgcgc gaacgtccgc
    10981 tcagtgccac ccagtctttt ttggcggcaa ccggcacgcc cttgccgcgt gtttcgttga
    11041 tggtggaggc gggcaggggc tctaggaggt tggctgcgga aagggtggcg agtgccggga
    11101 tttgttcgga atagaatacg tcggcggggc ttcggctgcc ttcttctttg atttgaccgg
    11161 caagctggtc gcctttggcg ctgttgagtt tgactttgat gccggtagcc cgggtaaagg
    11221 catctgcaac ggcttgtgcc gcttctttgt gttggccgtt gtacacggta atgtctgcca
    11281 gcgcgggggt tgcggcggtc agggctgcgg caagcagtgc gtatcggata gatgttttca
    11341 tatcgatttt ctcctaatga atgagagtgt acaccttgtt aaaacataac ggtgtgtagt
    11401 gtattccttc ttctttataa atgcaaataa ttatttttta aatttgttat tatccgatcc
    11461 ggttattgtt tgttctgact tgtatttttt ccgtgcatcg cgcccgtaag gcggaagcgg
    11521 cgggcaatgc gtggcggaat gtgggtaaag gcggcatttt gatttgtcgg aatgcttgag
    11581 aacccctctc tttaaaacag cgtctgaaca gggttgccgg aatagtattg ccatcccggc
    11641 agatacagtt tgtcggggtc ttgccaatat tgttcatcca gactgttcgg cagtgaggcg
    11701 gttttgttgt cgagatgttt tgttatatcc cacatttctt tcaggttttt accttccgat
    11761 tggaggtggc ggatttcttt tctcaatgcg tctgccgccc gatggatcag cattgcgctg
    11821 tgtaccgcgc cgattttttt cagaacggaa caagtctttt cggcggcggg gaaaccccag
    11881 ttgcagacaa attgcagtat gccgccgttg ccgatatcgg cttctgcccg ccaaattaaa
    11941 accagctcct gttcccaccc gtccatgctt tcgagtttgc cgtcatgccg ttcaaaaagt
    12001 ttgtccaccg cctgccacat catctgctcg aagcggtcgg cttgggtatc cgtatcggtc
    12061 atcgtgttct tgccttttaa aaatgccgtc tgaacatttc ttcagacggc atttgggggt
    12121 taagccaaca tttcccgcca gcgtttgact tggaaacgga cttgttcagg cgcggtgccg
    12181 cccaagtggt tgcgcgcgtt taagctgcct tcgggtgtca gcacgccgta aacgtcgtcg
    12241 gcaatcaaat cgctgaaacc ttgtaagact tcgagcggca gttcgctcaa atcgacgccg
    12301 gcttcatcag catggcgcac ggcttgggcg acgacttcgt gggcatcgcg gaaaggcatg
    12361 ccttttttga ccagataatc cgccaagtcg gtggcggtag cgaagccctg catcacggcg
    12421 gcgcgcatat tgtcgggttt gacggttacg ccgcgcatca tatcggcgta aatccgcaac
    12481 gtgtcgataa gcgtgtcggc ggtgtcgaac aagggttctt tgtcttcctg attgtctttg
    12541 ttgtacgcca agggttggga tttcatcagg gtaatcagac cgataaggtg tccgatgacg
    12601 cggccggatt tgccgcgcac gagttcgggc acgtcggggt ttttcttctg cggcatgatg
    12661 gacgaacctg tgcagaagcg gtcggcgatg tcgataaagc cgaaacgcgg gctcatccac
    12721 aaaatcaatt cttcagacag gcggctcagg tgaaccataa ccagcgaggc ggcggctgtg
    12781 aactcaatgg cgaaatcgcg gtcggatacg gcatcgagcg agttctggca gatttgttca
    12841 aagcccaata gctcggcggt gatttcgcgc tgaatcgggt aggtcgtccc ggcaagggcg
    12901 gctgcgccga gcggcatacg gttgacgcgg cagcggcagt ccgccatccg ttcgttatcg
    12961 cgtccgagca tttcgacgta ggcgagcata tggtgtccga agctgacggg ctgggcgact
    13021 tgcagatggg taaaaccggg catgacggtt tcggcgtttt gttccgccaa atccagcaat
    13081 gccgtctgaa ggctttgaat caggcttcgg atgacggtaa tctggtcgcg cagccaaagg
    13141 cggatgtcgg tggcgacttg gtcgttgcgg ctgcggccgg tgtgcaggcg tttgcccgcg
    13201 tcgccgattt tgtcggtcag gcggcgttcg atgttcatgt ggacatcttc caaatcggac
    13261 gaccattcga ttttgccgct gcggatttct tcgaggattt ccgccatgcc ccgacggatg
    13321 tctgccaaat cgccttcgtc caacacgccg gtttctttca acatttgcgc gtgtgccagc
    13381 gagccttgaa tgtcccattc ggcaagccgt cggtcgaaac cgatggaggc ggtgtattgt
    13441 ttgacgagtt cggaaacggg ttcgttgaaa cgtccggacc aggttttgtc gtgcataagg
    13501 attccttgat ggggttattc ggtgcggtat ttttccaaaa ggcggcggaa gggttcgccg
    13561 gtttcggggt gtttcaggcc gtaggcgacg gtggcttcga ggtagcccag tttgctgccg
    13621 cagtcgtagc gcgtgccttc aaaggggtgc gccaggacaa attcgtgatc gagcagcttg
    13681 gcgatgccgt ctgtaagctg gatttcgttg cccgcgccgc gcggcagtcc ggtcagtaag
    13741 tcgaaaatgc gcggggtaag gatgtagcgt ccgacgacgg caaggttgga gggcgcgtct
    13801 tcgggcttgg gtttttcgac aatgccgata atgcgttgga actgtttgag ctgttcggtt
    13861 tcgacgatgc cgtatgagcc ggtttgcgat gcttcaacgg tttctacgcc caaaatgctg
    13921 ttgccgctgc gcccgtacac ttcgaccatt tgtttgagcg cgcctttggg ggcatcaatc
    13981 aggtcgtcgg caaggataac ggcaaagggt tcgtctccga tggcggcgcg ggcgcacaag
    14041 acggcgtgtc ccaagcccag tgcttccgcc tgacggatgt agaggcaggt aatgttcggc
    14101 ggcaggatgt tgcggacgtg ttccaacaat ttgtctttat ggcgcatttc caactcggtt
    14161 tcgagttcgt atgccttgtc gaaatggtct tcgatgctgc gtttgttgcg tccggtaaca
    14221 aacaccattt ccgtgcagcc ggcttccacg gcttcttcta cggcgtattg gatcagcggc
    14281 ttgtcgacga tgggcagcat ttctttcggg cttgccttgg tggcgggcag gaagcgtgtt
    14341 cccatccctg cgacggggaa aacggctttc cgtatgggtt tcattctttt tcctttgtat
    14401 tgttttgatg tttaaagggc gagtttgcgt aagagttcgg caagtgcctg cgcgcggtgg
    14461 ctttcgcggt ttttgacctc cgaatccaat tcggcggcgg ttttgccgtg tccgggcaga
    14521 taaaaatacg ggtcgtaacc gaaaccgttt tcaccggcgg gaacgccgtt ccattgtccg
    14581 tgccatacgc cttcggcaat aatcggacgc gggtcgtctt tatggcggac aaaaaccaat
    14641 acgcagacgt agcaacagct tttgtcggct ttgccggcaa gttcggcggc aagtttcagg
    14701 ttgttggcgg tatcggattt gggattgctg ccggcgtaac gtgcggaatg tatgcccgga
    14761 gcaccgttta aggcggcggc acagatgccg ctgtcgtcgg cgagtgcggg cagcccgctg
    14821 tgtccggcgg cgtgccgggc ttttgccagc gcattttcaa caaaggtgga atagggttcg
    14881 gggcattcgg gtatgctgaa ctcggattgc ggcaatacgg taatgccgta tggttcgaat
    14941 aggttgccga attctttgag tttgcctgca ttgccgcttg ccaaaacgat tttttccggt
    15001 ttttcagaca tagcggtttt cctttgcggc ggattgggcg gcgcgtaggg atttgtgccg
    15061 caggtagagg gcgaggctgc cgatttgtcc gaacagtgcg ccgaatgcga aaagaaagga
    15121 ggcgacggcg aaggctttgc tgccgacggt cagcagataa gcacccaaga ggacgagcag
    15181 cataaatgcc agtgtgaaga gggcaacaga caacagatag atttttcggc ggttcatggc
    15241 gttcggtcgg aaacggtatg ttcggattat agccgattgg gacggtattc cctagagctt
    15301 ggaaaaatgc cgtctgaaac ggcgttcaga cggcatggcg ggcgttatgc ctgtttgttc
    15361 caacgttcga tggattcttt gatgactttt ttcgcttctt ccgcatcgcc ccacgattcg
    15421 actttggtcg tacctgcttt tttcaggtct ttgtaatggt tgaagtggaa ctcgatttgt
    15481 ttgatgagct gttgcggcaa atcggacaaa gttttgtagg cgttgccgtt gttgcggtcg
    15541 tcggcaggaa cgcagacgat tttgtcgtcc acttcgccgt cgtcaacgaa tttcatcacg
    15601 ccgataacgc gcgcttccaa gaatacgccg gttgccaaag gttgttcggt aacgagcagc
    15661 acgtccaatt cgtcgccgtc ttcgtccaaa gtttggggaa tgaagccgta gttggtcggt
    15721 ttggcgaaga tggcgggttc gacgcggtcg agttggaatg cggccagttt gcggttccat
    15781 tcgattttgt ggttgctgcc ggcggggatt tcgttgacaa cgttgatgat gccgccgtcc
    15841 acgtcgccgg gggtcaggat ttggttgaag tctgccattt ggtttccttt gtttgggaaa
    15901 gtgtgaagtt tgaaagtata gcacaaacgt ccggctgaaa atgcgcccga tgcctctgaa
    15961 agggtgtacg ggcgcgtgtt accgtttgcc caaaaacctg cccagttcca aaatcgcgcg
    16021 cctgttggac ggggaaaata ctttttccgc tgcttcttcc atatcgaacc aaccgtagga
    16081 gacgtgttct tcgggttgca gggcgatggg cgtatcacgc gggatttcgg cagagaagag
    16141 gtgttcgcgg ttttcaaaca cgccttttgg atagcggtgc cgccagtggt ggtagatttc
    16201 gtaaaccgtg ctgtcgtgcc agtcttgaag ctgcccgtcc gccagcagga tgccggtttc
    16261 ttcccaaact tcgcgccttg ccgtttgggc gacggtttcg cccggttcga ggctgccggt
    16321 taccgactgc caaaatcctt ccggatgcgt gcgttcgatg agcaggatgc cgccgtcccc
    16381 gctataaagg acgaccagtg cggaaacggg gtatttgagc ggttttgcca tcggcatctt
    16441 tcggcgggct gcggtaatga aggggcggat tatagcaaac gccgcacgtt atggcgttta
    16501 tccttttccg tatccttttt tctgcacgga tgggacgcgc cggtgtttgc cggtaaattt
    16561 tccgttgtgt caaaaagata agggcggttg tgattttaat gcttgccaaa gcgtcgggcg
    16621 gaaactataa tccgaaactt atcgagtcgg agtgtggcgc agtctggtag cgcacttgca
    16681 tggggtgcaa ggggtcgaag gttcgaatcc tttcactccg accaaaaatt ccgaaagccg
    16741 ctttcaaaag cggctttttt gccgtccgta tgattatgat gtagagtacg cggcgacaga
    16801 cattcaaatg ccgtctgaaa accgttcaga tggcatctct ttatcttagt ttcattccgt
    16861 accatcttaa ggaacatcaa attgggcatt tcccgcaaaa tatcccttat tctgtccata
    16921 ctggcagtgt gcctgccgat gcatgcacac gcctcagatt tggcaaacga ttcttttatc
    16981 cggcaggttc tcgaccgtca gcatttcgaa cccgacggga aataccacct attcggcagc
    17041 aggggggaac ttgccgagcg cagcggccat atcggattgg gaaacataca aagccatcag
    17101 ttgggcaacc tgatgattca acaggcggcc attaaaggaa atatcggcta cattgtccgc
    17161 ttttccgatc acgggcacga agtccattcc cccttcgaca accatgcctc acattccgat
    17221 tctgatgaag ccggtagtcc cgttgacgga ttcagccttt accgcatcca ttgggacgga
    17281 tacgaacacc atcccgctga cggctatgac gggccacagg gcggcggcta tcccgctccc
    17341 aaaggcgcga gggatatata cagctacgac ataaaaggcg ttgcccaaaa tatccgcctc
    17401 aacctgaccg acaaccgcag caccggacaa cggcttgccg accgtttcca caatgccggt
    17461 agtatgctga cgcaaggagt aggcgacgga ttcaaacgcg ccacccgata cagccccgag
    17521 ctggacagat cgggcaatgc cgccgaagcc ttcaacggca ctgcagatat cgttaaaaac
    17581 atcatcggcg cggcaggaga aattgtcggc gcaggcgatg ccgtgcaggg cataagcgaa
    17641 ggctcaaaca ttgctgtcat gcacggcttg ggtctgcttt ccaccgaaaa caagatggcg
    17701 cgcatcaacg atttggcaga tatggcgcaa ctcaaagact atgccgcagc agccatccgc
    17761 gattgggcag tccaaaaccc caatgccgca caaggcatag aagccgtcag caatatcttt
    17821 atggcagcca tccccatcaa agggattgga gctgttcggg gaaaatacgg cttgggcggc
    17881 atcacggcac atcctatcaa gcggtcgcag atgggcgcga tcgcattgcc gaaagggaaa
    17941 tccgccgtca gcgacaattt tgccgatgcg gcatacgcca aatacccgtc cccttaccat
    18001 tcccgaaata tccgttcaaa cttggagcag cgttacggca aagaaaacat cacctcctca
    18061 accgtgccgc cgtcaaacgg caaaaatgtc aaactggcag accaacgcca cccgaagaca
    18121 ggcgtaccgt ttgacggtaa agggtttccg aattttgaga agcacgtgaa atatgatacg
    18181 aagctcgata ttcaagaatt atcggggggc ggtataccta aggctaagcc tgtgtttgat
    18241 gcgaaaccga gatgggaggt tgataggaag cttaataaat tgacaactcg tgagcaggtg
    18301 gagaaaaatg ttcaggaaat aaggaacggt aataaaaaca gtaactttag ccaacatgct
    18361 caactagaga gggaaattaa taaactaaaa tctgccgatg aaattaattt tgcagatgga
    18421 atgggaaaat ttaccgatag catgaatgac aaggctttta gtaggcttgt gaaatcagtt
    18481 aaagagaatg gcttcacaaa tccagttgtg gagtacgttg aaataaatgg aaaagcatat
    18541 atcgtaagag gaaataatag ggtttttgct gcagaatacc ttggcaggat acatgaatta
    18601 aaatttaaaa aagttgactt tcctgttcct aatactagtt ggaaaaatcc tactgatgtc
    18661 ttgaatgaat caggtaatgt taagagacct cgttatagga gtaaataaaa atggcaattt
    18721 ggaattatcg gtactacctt atcccatcag ctgctatcag aaataaattt aatgcaaata
    18781 aagatatcat acttgatgag tatcgatcta atggttttca gaattttaat gagaataaaa
    18841 gttttgaaaa ttactttatc gataatgatg ttatattatt atcaataata aatgaagcaa
    18901 aaaaacagct taaattgaaa gaatcttggg ataaagacgc aatcatgttt tgtgataatt
    18961 ttggtaatag tcttaccgtt tggccagatg atatagagtg cgaacttgat ttaagatttg
    19021 attatactaa atttattcag aaaaccattg attgggcaat aaaatataat tgtctacttg
    19081 taatagaaaa aacaggaaat gtagtttccc ctaatataaa taatctgatg tatgaaataa
    19141 aagcatattt ggaaagcaag ccgtggccca tatgaaacct aaactcaaca agtaggatgt
    19201 gtgcggaacg cacgtatgcg gttctcaagg tttgagctaa gaggccgtct gaaaacagaa
    19261 aaactgtttc agacgacctt tcttttaacc agttgccaca gcaaccggac aaaagcagcc
    19321 tacctccaca tccatatagg caatacaggg gagatatttt gtaaattcta cgaatatttt
    19381 acctgctaaa cagggtagga tatggtatga agcgaacatt ggcttaataa acactatgtc
    19441 aagatcgaat caggctggta ctagattgtt gtattccaat tatggattgc tatatataac
    19501 aactgatcat tatatctctg caacacggtt tgtagcttgg aaataggagt ataacttatg
    19561 caattagaga ttatcggtag taaaatttat acggaacaag attttcataa tcaaatttca
    19621 aaaatatttt ctatacaaga ttattatggg aacaatcttg atgctttatg ggatttatta
    19681 agcacaaatg tagaacgacc gattactttg gtatggaaag atgctatgtt ctcaaaaaat
    19741 caattagaaa atatatttat tgaaatcgta aatgttctag aaagagttaa gaaacaagat
    19801 gaggattatg gattcgaaga aaaatttaat tatattttag agtaagtaaa ccctaattac
    19861 attacgtaca caggcttaaa actccccaga gccaattaag caagccgtaa cccatataaa
    19921 acttaaactc aacaagtagc atgtgtgcgg aacgtacgca tgcggttctt aaagtttgag
    19981 ctaagaggcc gtctaaaaac agaaaaaccg tttcagacgg tctttgttta acgccaccga
    20041 tccagcgggt tacaaagcgc agtcaatgcc gctgcgcctt atgcctccga agcaataggc
    20101 agaacatttg gacacggtga aaacaaaaac gaaaccgccc aagccgtcgg acatttcctt
    20161 ttaggagcag ctattgcccg cgtcaacggt ggtaattttg ctgccggcgg ctcggcagca
    20221 gttgcagctg aaaaggcggc ggaacatctt gcccaacagt ataacgacgg taaaaccgca
    20281 atcgatccgc aaacaggcga gttcaatgcc aacctgctgc cggaacatat caaagaggaa
    20341 atcaaatcaa agagcggggt gattgcatcg ctgacgggcg cggccgtggg cggcacgccg
    20401 gtagatgcgc aaaccggagg tgcggtcgga cagaatgcgg tggaaaacaa cctctatctg
    20461 acatcggaag ccttaaagaa ggacaagcag acagctcgta aaatttattc cgtcataaaa
    20521 gagcaagtca agcatgaatg cagttccaca ggaagaatta ccgaatgtcg tcaaaatata
    20581 ggacgcatta tcgaatttac ccaagacaaa cgctttgaca gtaggtttaa ggacttaaaa
    20641 aaagaatcct tatattacct aaataaacat cctgatttag tagcctctta tttgaaggct
    20701 gaatacgaaa agctggatag ggaagacaaa agtatcctgc accgctacat ctcacccggg
    20761 gctgaaatcg tttcgggcag tttgggggtt gttctttcag gagtagccgg aggcggatct
    20821 tgtgccgaga ctttcggctt aggctgtgcc gccgctttgg ttggtgtaac gtcttcctac
    20881 gatcatgtca ttactgggac gaaaaacttc ggaaaaaaag ccagcgagca acgaccgacg
    20941 attgcggttc aggccttgaa gcagttgggg ctgtcggagc aggctgcgga atatgttcag
    21001 ttctctatag atttgttcag tgtgggtaaa tcggggggcg gtatacctaa ggctaagcct
    21061 gtgtttgatg cgaaaccgag atgggaggtt gataggaagc ttaataaatt gacaactcgt
    21121 gagcaggtgg agaaaaatgt tcaggaaacg agaagaagga gtcagagtag tcagtttaaa
    21181 gcccatgcgc aacgagaatg ggaaaataaa acagggttag attttaatca ttttataggt
    21241 ggtgatatca ataagaaagg cacagtaaca ggagggcata gtctaacccg tggtgatgta
    21301 cgggtgatac aacaaacctc ggcacctgat aaacatgggg tttatcaagc gacagtggaa
    21361 attaaaaagc ctgatggaag ttgggaggtg aaaacgaaaa aaggtgggaa agtgatgacc
    21421 aagcacacca tgttcccaaa agattgggat gaggctagaa ttagggctga agttacttcg
    21481 gcttgggaga aaagaaagga attagaaggt caaaaatgga gaggtacaag taaatctggt
    21541 gttgaaatag aaggatttac ggaacctaat cgaacagctt atccacttta taatagtaaa
    21601 tgaagtattg attaaaaagg tgtttttaaa tgtataaaat aaaatgtttt tatgatcagt
    21661 ttagttctgg tgaattattt aactactgcc agtttttaga taattcttct aatcaaaaga
    21721 taaatacacg aggaacggtg tatcaatata t
//
LOCUS       DAWKST010000013        21695 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.013, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000013 DAWKST010000000
VERSION     DAWKST010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21695
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.013"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            54..1094
                     /gene="murB"
                     /locus_tag="WJL87_000341"
     CDS             54..1094
                     /gene="murB"
                     /locus_tag="WJL87_000341"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ7798825.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADGLRDIVRLPEFN
                     RDTVLWLGGGSNILLMEDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHA
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            1106..1756
                     /locus_tag="WJL87_000342"
     CDS             1106..1756
                     /locus_tag="WJL87_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ7798826.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MITKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHREKYDRKIGNGNP"
     gene            1811..2587
                     /locus_tag="WJL87_000343"
     CDS             1811..2587
                     /locus_tag="WJL87_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ7798827.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLGSTDAFQTALWRHHADKPVWFFLLPPSSLTHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDTARECDETATPDPQTESARQILAAEQHLLDSGISNIDILRLGG
                     LYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFAPDDSMGKIIRTVCDNSLSL"
     gene            2638..3204
                     /locus_tag="WJL87_000344"
     CDS             2638..3204
                     /locus_tag="WJL87_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ7798828.1"
                     /translation="MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            3224..4114
                     /locus_tag="WJL87_000345"
     CDS             3224..4114
                     /locus_tag="WJL87_000345"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ7798829.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            4139..4897
                     /locus_tag="WJL87_000346"
     CDS             4139..4897
                     /locus_tag="WJL87_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ7798830.1"
                     /translation="MNPKISSDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NRWEGDTGRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(4952..5617)
                     /locus_tag="WJL87_000347"
     CDS             complement(4952..5617)
                     /locus_tag="WJL87_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ7798831.1"
                     /translation="MTVLDREQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(5730..6626)
                     /locus_tag="WJL87_000348"
     CDS             complement(5730..6626)
                     /locus_tag="WJL87_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ7798832.1"
                     /translation="MQPPKASDIISHLQIDGIFPGGNAIPCTHLNNYMNIKEKQLMNT
                     IADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTVARLTMTVYLPAEQKGTHMS
                     RFVALMEQHAEALDFAQLRRLTAEMVALLDSRAGKISVSFPFFRKKTAPVSGIQSLLD
                     YDVSLTGEMKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADAEVGI
                     EEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATSLIADKRIKSF
                     VVESENFESIHNHSAYAYIAYP"
     gene            6694..7851
                     /locus_tag="WJL87_000349"
     CDS             6694..7851
                     /locus_tag="WJL87_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ7798833.1"
                     /translation="MKFATQAIHSGYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQVLEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLQPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNLDNLDKLLKAHKVKLVWLETPSNPLLRLVDIKALAA
                     KAKAAGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPMDCWLVLRGIKTLALRMNAHCQNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYELAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(7914..8915)
                     /gene="hemB"
                     /locus_tag="WJL87_000350"
     CDS             complement(7914..8915)
                     /gene="hemB"
                     /locus_tag="WJL87_000350"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ7798834.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAVANGWLDGDKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(9098..9382)
                     /gene="yhbY"
                     /locus_tag="WJL87_000351"
     CDS             complement(9098..9382)
                     /gene="yhbY"
                     /locus_tag="WJL87_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ7798835.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            9492..10112
                     /locus_tag="WJL87_000352"
     CDS             9492..10112
                     /locus_tag="WJL87_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ7798836.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINEKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFEGLLNNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            10176..12143
                     /gene="ftsH"
                     /locus_tag="WJL87_000353"
     CDS             10176..12143
                     /gene="ftsH"
                     /locus_tag="WJL87_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ7798837.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQIA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            12364..12795
                     /locus_tag="WJL87_000354"
     CDS             12364..12795
                     /locus_tag="WJL87_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ7798838.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSVTLVEAFVKEADRRYA"
     gene            12788..13066
                     /locus_tag="WJL87_000355"
     CDS             12788..13066
                     /locus_tag="WJL87_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ7798839.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            13119..13697
                     /gene="pth"
                     /locus_tag="WJL87_000356"
     CDS             13119..13697
                     /gene="pth"
                     /locus_tag="WJL87_000356"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ7798840.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIGHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            13758..13964
                     /locus_tag="WJL87_000357"
     CDS             13758..13964
                     /locus_tag="WJL87_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ7798841.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            13961..14323
                     /locus_tag="WJL87_000358"
     CDS             13961..14323
                     /locus_tag="WJL87_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEZ7798842.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     LTGALLALFAARSADRPD"
     gene            14554..15969
                     /locus_tag="WJL87_000359"
     CDS             14554..15969
                     /locus_tag="WJL87_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13/DASS family transporter"
                     /protein_id="HEZ7798843.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVALLRLSRGSMKAAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDREKERKTYVFVLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSSAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            16088..16597
                     /locus_tag="WJL87_000360"
     CDS             16088..16597
                     /locus_tag="WJL87_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ7798844.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            16760..18142
                     /locus_tag="WJL87_000361"
     CDS             16760..18142
                     /locus_tag="WJL87_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ7798845.1"
                     /translation="MANIAHDIFKAYDIRGIVGKTLTDEAAYLIGKAIATKASEKGIT
                     RIALGRDGRLSGPGLMAQIQHGFTDSSIDVLNVGMIATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIVEKDGFVAADKQGSVTEKDISGEYHNNI
                     VGHIKLKRPMKIVIDAGNGVGGAFAGKLYKGLGNEVAELFCEVDGNFPNHHPDPSKPK
                     NLQDLIAALKNSDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPKAK
                     VIFDVKSTRLLTPWIKEHGGEPVMEKTGHSFIKSAMKKTGALVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(18162..18917)
                     /locus_tag="WJL87_000362"
     CDS             complement(18162..18917)
                     /locus_tag="WJL87_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ7798846.1"
                     /translation="MIKIRNIHKTFGESTILRGINLDVGKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(18927..19643)
                     /locus_tag="WJL87_000363"
     CDS             complement(18927..19643)
                     /locus_tag="WJL87_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ7798847.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(19633..20460)
                     /locus_tag="WJL87_000364"
     CDS             complement(19633..20460)
                     /locus_tag="WJL87_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7798848.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSESGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLSDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            20745..21653
                     /locus_tag="WJL87_000365"
     CDS             20745..21653
                     /locus_tag="WJL87_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ7798849.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALTA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
BASE COUNT         5348 a         6386 c         5355 g         4606 t
ORIGIN      
        1 aaacgatggg ctgaagcccg ccactggcaa tactcatttt aaagttcaaa cccatgcaac
       61 ccatccgata ccgaaccgac cttaccccct acaacacttt cggcctgcgc gcccaagccc
      121 gagcctttat tgcgcttgaa cacgccgacg ggttgcgcga catcgtccgg ctacccgagt
      181 tcaaccgaga tactgtttta tggctgggcg gcggcagcaa tatccttttg atggaggact
      241 acgccggtct ggtcgtacat atggaaaaca aaggcatacg cgaaattgct cgttcagacg
      301 gcatggttct gattgaagcg caggcgggcg aaatctggca cgattttgtc ctgcacgccg
      361 tcgcgctggg tttgaacggt ttggaaaacc tgagcctgat tccgggtacg gtcggcgcgt
      421 cgcccgtgca gaacatcggc gcatacggcg tagaggcgaa agatgtgatt cacagcgtgc
      481 gctgcttcga tttggatacg gaaacctttg taaccctttc caatgccgac tgccgcttcg
      541 cctaccgcga aagcctgttc aagcaggaag gtaaagggcg ttatgtgatt gtttcggtcg
      601 tatttgcatt aaaaacgcat tttgtgccga acttgggtta cggcgatttg gcggccgccg
      661 ttgccgaact gagcgcgggc agggtgccga cggcgaaaga tgtttccgat gcagtgtgtg
      721 caatccgcaa cagtaaactt cctaatccta acgtgcttgg caatgtcggc agtttcttta
      781 aaaaccccgt cgtcagcgca gaaaaagccg ccaccttgtt gcagcggcat cctgatatgc
      841 cgcgctatcc gcagcccgac ggttcggtca aactcgccgc cggctggctg atcgaccaat
      901 gtcgtctgaa aggctttcaa atcggcggcg cggcggtaca tgacaggcag gctttggtct
      961 tagtgaacaa aaacaatgcc tcggcaaacg atgtccggca gttggcgcaa cacatcaaat
     1021 ttacagtatt tgctcggttt caggtagaat tgcacgccga acccaattgg ctgcctgctt
     1081 cgttcagcct gtaaatccaa ggagtatgcc cgtgacccgc cccgccaaaa tcaatactta
     1141 cacacgcatc atcgacgcca gccttgcgct tttcaacgag gaaggcgagc gcaacatcag
     1201 caccaaccat attgccgccc acttgggcat cagtccgggc aacctctatt accacttccg
     1261 caacaaagac gaaatcatcg tccaactgtt caaacgttac agcgaagccc tgctggaata
     1321 cctcaacgaa gccgtgttgc cgtctgatgt ggaagactcc atcaattata tggcgggcat
     1381 ttacgacgtg atgtgggaat accgcttcct attcagcgac gtgaacaccc tgcttgcacg
     1441 cagtgccgaa ctattgggcg aacacaatac cttcacccaa gccaaagtct ccccgctctt
     1501 ggtcaacctg ctgacccagc tcaacggtct gaacatcatc caagccgacc aaaccgccat
     1561 gaacgacctc gccgtcaata tgtggatgat cacgaaatac tggttcgact tcgacagctc
     1621 cctgcgcggc cgcaccaagc tgaccgaaga ctccaaagca cgcggcatcc gccgcacctt
     1681 aagcctcctt cgtccttatc ttttgcccga acaccgcgag aaatacgacc ggaaaatcgg
     1741 caacggcaac ccgtaaaccc acagcgtccg aatcagcctt cagacggcac ttacccgacg
     1801 gtatcagcga atgcgcgcag tccccccgcc ccatatctcc atcacaggcc taggttacct
     1861 cggcctgccg ctggcacaaa agttttacca acacggcagc cgcgtcgccg ccataaaacg
     1921 cagcctgact tcggacgaca tcaacctgcc catacatctc gacaccttcg acctcggcag
     1981 caccgatgcc tttcagacgg cattgtggcg gcaccacgcc gacaaacccg tctggttctt
     2041 cctgctaccg ccctcctcgc tgacgcatta cgccgacacc gtcaaacaat gggcagaact
     2101 tgcccgggcg tgcaacgtgc aacacctgat tttcacaagc agcaccagcg tttacggcga
     2161 tacagcgcgc gaatgcgacg aaaccgccac acccgatccg caaaccgagt ccgcccgcca
     2221 aatcctcgcc gccgaacaac acctgctcga cagcggcatc tccaacatcg acatcctgcg
     2281 gctgggcggg ctttattgcg ccgaacgcca tcccgtcagc cgccttgttc aaaagcaaaa
     2341 catcccgggc ggcaaccgcc ccatcaacat cgtccaccgt aatatcgccg tcgaaagcct
     2401 gtttcagacg gcatttaacc ccggcggcag gcggctgaaa aacattatcg aaccgcgcca
     2461 cccgacacga cgcgaattct acacggaaga agccgccaaa ctcggtttgc cggctcctga
     2521 tttcgcaccc gacgacagca tgggcaaaat catccgtacc gtttgcgata acagcttaag
     2581 cctgtaaaat aagtgtcaac agcaacaaac aggcattctc cgccccgatc agaaaatatg
     2641 tccgccctcc tccccatcat caaccgcctg attctgcaaa gcccggacag ccgctcggaa
     2701 cttgccgcct ttgcaggcaa aacactgacc ctgaacattg ccgggctgaa actggcggga
     2761 cgcatcacgg aagacggttt gctctcggcg ggaaacggct ttgcagacac cgaaattacc
     2821 ttccgcaaca gcgcggtaca gaaaatcctc caaggcggcg aacccggggc gggcgacatc
     2881 gggctcgaag gcgacctcat cctcggcatc gcggtactgt ccctgctcgg cagcctgcgt
     2941 tcccgcgcat cggacgaatt ggcacggatt ttcggcacgc aggcagacat cggcagccgt
     3001 gccgccgaca tcggacacgg catcaaacaa atcggcagga acatcgccga acaaatcggc
     3061 ggattttccc gcgaatccga gtccgcaaac atcggcaacg aagcccttgc cgactgcctc
     3121 gacgaaataa gcagactgcg cgacggcgtg gaacgcctca acgaacgcct cgaccggctc
     3181 gaacgcgaca tttggataga ctaaccttca gacggcatcc gacatgaaca gcccttttca
     3241 caacatcggc atcgtaaccc gccccaacac gcccgacatc caagacaccg cacacacgct
     3301 gattaccttt ttgaagcagc acggctttac cgtctatctc gacgaagtcg gcataaagga
     3361 aggctgcatc tatacccaag acaccgtcgg ctgccatatc gtcaacaaga ccgaactggg
     3421 gcaatactgc gacctggtcg ccgttttagg cggagacggc acctttctct ccgtcgcccg
     3481 cgaaatcgcc ctgcgcgccg ttccgattat cggcatcaac caagggcatt tgggcttcct
     3541 gacccaaatt ccccgcgaat atatgacgga caagctattg cccgttttag aagggaaata
     3601 ccttgccgaa gagcgcatcc tgattgaggc cgcactcatc cgcgaaggca aaaccgccga
     3661 acgcgccatc gccctcaacg atgccgtcct ctcccgtggc ggtgccggac agatgattga
     3721 gtttgaagtc ttcgtcaatc gggaattcgt ctatacccag cgttcggacg ggctgattgt
     3781 ctccaccccc accggatcga ccgcctattc gcttgccgcc ggcggcccca tcatgcaggc
     3841 aggattacac gccttcacgc tcgtccccat ctgcccgcaa tccatgacca accgccccat
     3901 cgccattcca gacacgtccg aaatcgaaat cctcgttacc caaggcggcg acgcgcgcgt
     3961 ccatttcgac ggtcaaaccc atatcgacgt gcaaaacctc gaccgcatca ccatccgccg
     4021 ctaccgaaat cccttacgca ttttgcaccc gaccgactac caatatttca aaaccctgcg
     4081 ccaaaaactg cactggggcg agcaattagt ctaagccggc ctttacccga aagtatccat
     4141 gaatcccaaa atttcctccg accatacaga agatgccgtg cgcctctcca aacgcatggc
     4201 gcaactgggg ctttgttcgc gccgcgaagc cgacggctac atcgaacagg gctgggtaac
     4261 ggtcaacggc aaaaccgccg tactcggtca gaaagtttca ccggccgacc gtatcgaact
     4321 gaacaagaaa gcccacgaac agcaggcggc acgcattacc atcctgctga acaaacccgt
     4381 cggctatgtc agcgcacaag cggaaaaagg ctataaatcc gccgccgaac tgattacccc
     4441 tgaaaaccgc tgggaaggcg acaccggccg catccgtttc gatccgaaac acaaaatcgg
     4501 cctcgccccc gccggcaggc tcgatatcga ttcagtcgga ttactggtat tgacgcagga
     4561 cggccgtatc gccaagcagc ttatcggcga aaacagcggc agtgaaaaag aatatttggt
     4621 gcgcgtgcgc ggcaaattgg acgaaaaagg acttgcctta ctgaatcacg gattgagttt
     4681 ggacggcgag aaactgcgtc ccgcccaagt agaatggcaa aacgaagacc aactgcgctt
     4741 cgtgttgaaa cagggtaaaa agcggcaaat ccgccgtatg tgcgaactgg tcggactgcg
     4801 cgtcgtcggg ctgaaacgca tccgcatggg caaggtcaaa ctcggcaggc tgccgcccgg
     4861 caaatggcgt tatcttgctc ccggcgaatc gttttaaata agcattaaaa aatgccgtct
     4921 gaaaatcctt gcgttttcag acggcattgc cttatgccca aataacggtt tcttccaagg
     4981 gcctgcgcgc cttcgtggcg atttcctgaa cgcggtaacc gaatgtcgcg gcgacggaca
     5041 cgccccattc tgccgcatcg aacaaaccga actgcccgga caatacgcgt tccatatcgg
     5101 catagttgaa accttccacc gggcaggaat cgatacctgc catcgccgca cccgtcatca
     5161 tgttgcccaa cgcgatatag gtctggcggc aacaccagtc aaacaaggcg cgcgaatcgt
     5221 ccaaaatctt gatgtcgtcg gcttgaaacg cctgatacct cgccaaagat tttgccatgg
     5281 catccggttc ggtaacgccg cgccgtttga ggctttccaa cataaacggg ctgtcgaagc
     5341 gggcattttt ctttgccaaa aacaccacca aatgactggc ggtatccaaa gcatccgcca
     5401 taccccaaga aaacggcttg attgcctgtc ggatttcagg gttttgaacc acaacaaact
     5461 gccaaggctc cgaaccgacc gaactgggcg acaaacgccc gagttctaaa ataaactgaa
     5521 aatcctcggc actgatttta cgtgccgcat cgtaatgccg gcatgatttg cggtttttaa
     5581 atgcggatag aacctgctct ctgtctaata ccgtcatagt gcatccttta ttacttttat
     5641 tgcaaattga tttcaaaccg atacggcggc agattcgcca ataacgccca aacgcctttc
     5701 agacggcatt tggttcatcg caaacacgcc tacgggtagg cgatataggc ataagccgaa
     5761 tggttgtgga tagattcgaa attctcactc tcgacgacga aactcttgat gcgtttgtcg
     5821 gcaatcagcg aagtagcgac atcgcgcacc atatcttcca cgaatttcgg gttttcgtag
     5881 gccttttcgg taacgtattt ttcatcgggg cgtttgagca ggccgtagag ttggcagctc
     5941 gcctgcgcct ccacataatc gatgacttcc tcgataccga cttcggcatc ggcagtcagg
     6001 ctgacggtaa cgtgcgaacg ctggttgtgc gcgccgtatt gggaaatttc tttggaacac
     6061 gggcaaagcg aggttacggg aatcatgacc ttcatactgt ggccgtatgc accgtctttc
     6121 atttcgcccg tgaggctgac atcataatcc agtaaagact gaataccgga aaccggggcg
     6181 gttttcttgc ggaaaaatgg gaaagaaacg ctgattttgc cggcgcggga atccaaaagc
     6241 gcgaccattt cagcagtcag cctgcgcaat tgtgcaaaat ccaaggcttc ggcatgttgc
     6301 tccatcaatg cgacaaagcg cgacatatgc gtacccttct gctcggcggg caggtaaacc
     6361 gtcatggtca gacgggcaac tgtggactgg atgccttctg cagtttgcag ggtaatcgga
     6421 aagcgcaggt ctttgatacc gacctgattg atcggcagat tgcgcaaatc tcggctggat
     6481 tgcacgtctg caatagtgtt catgagttgt ttttccttaa tattcatgta attgttgaga
     6541 tgtgtgcatg gtatggcgtt tccgccgggg aatataccgt caatctgcaa atgcgagatt
     6601 atatcacttg cttttggcgg ctgcattgat tgtttctatt ttcgggatag tgataaaatc
     6661 ccgacttatt catatcttac aaggcagaaa cagatgaaat tcgccaccca agccatccat
     6721 tccggctacg attgcgacga acacaaccgc gcgctgatgc cgccgattta tcaaaacagt
     6781 atgtttgcgc tgcacgaaat cggcgaaaat gtgccttacc gctattcacg tctgagcaac
     6841 ccgacccgtc aggtattgga agataccgtt gccgatttgg aacacggtgc ggcaggtttt
     6901 gcgttttcca gcggtatggc agggattgat gccgtatggc gcaccttcct gcaaccgggc
     6961 gataccattg tcgccgtcgc cgatatttac ggcggcgcgt atgatttatt ggtcgatgtt
     7021 tatcaaaaat ggggggtgaa cgttgttttt gccgatttgg gcaatctgga taatttggac
     7081 aagctgctta aagcgcacaa ggtcaaactg gtttggctgg aaacgccgtc caatccgctt
     7141 ttgcgcttgg tagacatcaa agcgcttgcc gcgaaagcca aagcggccgg cgcactggtc
     7201 ggcatcgaca acacctttgc cacgccgtat ctgcaacagc ccttggatat gggttgcgat
     7261 tttgtgttcc attccgctac caaatatttg tgcggccatt ccgacgtgtt gatgggcatc
     7321 gtcgttgcca aaaccaaaga gctggcgcag cctttgcacg atatgatggt gcataccggc
     7381 gcggttgccg gcccgatgga ctgctggctg gtgttgcgcg gcatcaaaac actggctctg
     7441 cgcatgaacg cccattgcca aaacgcactc gaaatcgcgc gccgtttgga agcccatcct
     7501 gccattgaaa aagtgttcca tcccggcctg ccgtctcacg aacattatga actcgcccaa
     7561 gcgcaaatgc ctaaaggcat cggcggcgtg gtgacggttt atctcaaaaa cgacacgcgc
     7621 gaagcggcaa acagcgtgat taaaaacatg aaactggtca aaatggcttc cagcctcggc
     7681 ggcgtggaaa gtttggtcaa ccattgctat tcccagtccc acagcggcgt gccgcataat
     7741 gtgaaaatgg aaatgggcat caaagtcggc ctgctgcgtt tctccatcgg catcgaagac
     7801 gcggacgata tttggaacga tatttccgcc gcactcgata caactttgta aaccgtaaaa
     7861 atgccgtctg aaaccatagt ttcagacagc atttcaatat tgcgaatcaa atatcaacgc
     7921 ttcagcatct tggccgcctc aatggcataa taggtcaaaa tcccatccgc gcccgcacgt
     7981 ttgaatgcca gcaggctttc caaaaccact ttatcgccgt ccagccatcc gttggcaacc
     8041 gccgcctgca gcatcgcata ttcgcccgaa acctgatagg cataagtcgg cacaccgaac
     8101 tcgtccttca cgcggcggac aacgtccaaa tacggcaaac cgggcttcac catcaccata
     8161 tccgcacctt cctgaatatc gagcgctact tcatgcagcg cctcatcggt gtttgcggga
     8221 tccatctgat aggtcttttt atctgccttg cccaaattgc ccgaactgcc taccgcatca
     8281 cggaaagggc catagaatgc agaagcatat ttggcggaat atgccataat ccgcgtgtgg
     8341 atatgtccgg catcctccaa cgcctcgcga atcgcaccga tacgcccgtc catcatatcc
     8401 gacggagcaa ccacctgcgc gcccgcttca gcgtggcaca aagcctgctt gaccaaaacc
     8461 tctacggttt catcgttcat cacataaccg ttttcgtccg tcagcccgtc ctgaccgtga
     8521 accgtataag gatcgagcgc gacatccgtc ataatgccca gttcgggaaa cctctcgcgc
     8581 aaggcgcgga cagttgacgg cacgagtcct tcgggattgt acgcctcctg cgcacgctcg
     8641 gttttgtttg ccgtaaccac ggggaacagt gccaacatcg gaataccgag ctttaccgcc
     8701 tcttccgccg taaacagcag cctgtccaaa ctttgacgct tcacacccgg catagaaggc
     8761 acatcctcct cgcgcgccga cccctccaat acgaacaccg gataaatcaa atcatcggcg
     8821 gtcagcgtgt gttcgcgcat caggcggcgt gaaaaatcgt ccctgcgcat acggcgcata
     8881 cgcgaagccg gaacattgcg gtaaggaaac tgcataagcc ctccaatcat cctgtcaaca
     8941 attcaaacca tacggaagcc gccgcaactt gcagacagca tcgggcggca atcaaaatcc
     9001 gttttcaccg gaaagggttt cggcccgccg aaatgccgcg ccgaaggacg gcgtcccgcc
     9061 cattaaagaa caatagcaaa acaacagctt caggctgtca gccttcgata ttcttacgcc
     9121 acaataccaa aagtttcccg atatgctgaa caggttgcgc gtcaaccgcc tcacataagg
     9181 cagtgcagat ttcgatacgt tcggcacggt cgtcgccgaa tacgcgcact ttaatcagct
     9241 catgcgccgt caatgccgca tcggtttcct tgatgaccgc gtccgtcaga ccctgctgac
     9301 cgaccatcac aacaggatgg agatggtgcg cgcgcgcttt cagttccaaa atttctttgg
     9361 tgttcaattt ggtatccgtc attttcctac tgcttgaata aaaagaataa tgcggcattg
     9421 tacgcgattt ggcacgacat cggcaaaatt aacgtacaat acgcggtttc cattccgacc
     9481 cgcgaaccac tatggctgta cgttccaaat cctcaaaagc gtggctgcac gaacacgtca
     9541 acgaccacta cgtccatatg gcgcaaaaag acggctaccg cgcccgtgcc gcatacaaac
     9601 ttttggaaat caacgaaaaa gacaaattaa tcaaacccgg cacggtactt gccgacttgg
     9661 gcagcgcacc gggaagctgg tcgcaggttg ccgccaagct gacgggtact tccggagcag
     9721 ttttcgcctt ggacatcctg cctatggaag ccataggggg cgtctccttc attcagggcg
     9781 acttcaggga aaacgacgtt ttggcgcaat ttgaaggctt attaaacaac cgccccttag
     9841 accttgtaat ctgcgatatg gcgcccaata tgtcgggaaa cgccgtaagc gatcaggcgc
     9901 gcagctttta tttgtgcgaa ctggctttgg acttcgcctc gcaacacctg aaaaccggcg
     9961 gcagcttttt ggtcaaagtc tttcagggtg caggctatca ggaatacatg gcagccatgc
    10021 gcgaaatttt cggcacggtg cagacgcgca aacccgaagc ctcgcgcaat cgctccagtg
    10081 agatttattt attgggcaaa aataaacgct gacaatacag acggcgtgct ttacaatcat
    10141 ttccgtttta cacctcaatt atggagcctt gctaagtggg gaacaccttt aaatcaatcc
    10201 ttgtctgggt cgccttgggt atcggcctga tggctgcgtt caacgcttta gacggtaaaa
    10261 aagaagacaa cgggcaaatc gaatattctc agttcatcca acaggtcaac aacggcgaag
    10321 tatccggcgt caacatcgaa ggatccgtcg tcagcggcta cctgattaag ggcgagcgca
    10381 ccgacaaaag caccttcttc accaacgcgc ctttggacga caacctgatt aaaacactgc
    10441 tcgacaaaaa cgtccgtgta aaagtaacgc cggaagaaaa accgagcgcg ctggctgccc
    10501 tgttttacag cctgctgccc gtcctgctgc tgattggcgc gtggttctac tttatgcgta
    10561 tgcagacggg cggcggcgga aaaggcggcg cattctcatt cggcaaaagc cgcgcccgcc
    10621 tactggacaa agatgccaac aaagtgacct ttgccgatgt cgccggctgc gacgaagcca
    10681 aagaagaagt gcaggaaatc gtcgattacc tcaaagcgcc gaaccgctat caaagcctgg
    10741 gcgggcgcgt gccgcgcggc atcctgctgg cgggcagccc gggtacgggt aagacgcttt
    10801 tggcgaaagc gattgcaggc gaagccggcg tgccgttctt cagcatttca ggttccgact
    10861 ttgtcgaaat gttcgtcggt gtcggtgcaa gccgcgtccg cgatatgttc gagcaggcga
    10921 agaaaaacgc cccctgcatc atctttatcg acgagattga cgcagtcggc cgccaacgcg
    10981 gcgcaggttt gggcggcggc aatgatgagc gcgagcaaac attaaaccaa ttgttggttg
    11041 aaatggacgg ttttgagagc aatcagactg taattgtgat tgcggcaacc aaccgccccg
    11101 acgtactcga tcctgcgctg caacgccccg gccgtttcga ccgccaagtc gtcgtccccc
    11161 tgccggacat ccgggggcgc gaacagattt tgaacgtcca ttctaaaaaa gtgcctttgg
    11221 acgaatctgt ggatttattg tccctcgcgc gcggcacgcc gggtttttcc ggtgcggatt
    11281 tggcaaacct ggttaacgaa gccgccctgt ttgccggccg ccgcaacaaa gtcaaagtcg
    11341 atcaaagcga ttttgaagac gcaaaagaca aaatctatat gggtccggaa cgccgcagta
    11401 tggtgatgca cgaagacgaa aaacgtgcga cggcgtatca tgagtccgga cacgcgattg
    11461 ttgccgaaag cctgcccttt accgaccccg tccacaaagt aaccattatg ccgcgcggac
    11521 gtgcgctggg tctgacttgg cagcttccgg agcgcgaccg catcagtatg tataaagatc
    11581 agatgttgag ccagctctcc atcctgttcg gcggacggat tgccgaagac atcttcgtcg
    11641 gacgcatctc caccggcgca tcaaacgact ttgaacgcgc aacccaaatg gcgcgcgaaa
    11701 tggtaacgcg ctacggcatg agcgacaaaa tgggcgtgat ggtttatgcg gaaaacgaag
    11761 gcgaagtctt cttaggacgc agcgtaaccc gttcgcaaaa catttccgag aaaacccagc
    11821 aggacatcga tgcggaaatc cgccggattt tggacgagca gtatcagatt gcctacaaaa
    11881 tcctcgatga aaaccgcgac aagatggaaa ccatgtgcaa agccctgatg gaatgggaaa
    11941 ccatcgaccg cgatcaggta ctggaaatca tggcgggcaa acaacccagt ccgcccaagg
    12001 attacagcca caacctgcgc gagaatgcgg acgcggcgga agataacgcg ccgcacgctc
    12061 cgactcggga agaaaccgaa gcacctgccc cggcagacac cgcttcgaca gagtccgagc
    12121 agcagcctga aaacaaggct taacttcccg aacaaacggc agcccgcaag ctgccgtttt
    12181 cccatcctgt tttcagacgg cattttccgc tcaatgccgt ctgaaacgcc aaagccgtat
    12241 cggtttttgc ggatctgcga tatggcggtt taaattgaaa tcagacgagg caaggctgta
    12301 ccggtttaaa attaaaccgc tataataccg cccttcgaat acacgcccgc attggtgatg
    12361 cctgtgaaaa aagttgaaaa aaacatcttg gtgctgcacg gcgcggacaa aatgttcgag
    12421 ctggtcgata aggttgaaga ctatccgcac tttctgccgt ggtacagcaa gaccgaggtc
    12481 atcgggcgta gcggcaacga actgaaggcg cggctgttta tggactatat gcacgttcgc
    12541 caatcattcg ccacgcacaa ccgcaacatt ccgggcaggg aaatccgtat ggaactgctc
    12601 gagggtccgt tcaaaacctt acgtggaacg tggaaattta tcgatttggg cgacgatatg
    12661 tgcaaaatcg aattcaattt ggaatacgat ttttccaatg ccgttttgtc cgccttaatt
    12721 tcccccgtct tcaaccacct ttccgtcacg ctggtcgaag cgttcgtcaa agaggcagac
    12781 cgccgttatg cttgaaattg agattgtgta cgggctgccc gaccgacagg ttttgaaaac
    12841 catgcagctt gccgaaggaa caaccgtccg cgccgccgca ctgcaaagcg gtttggacgg
    12901 catatttgaa gatttaaacc tgcattccgc gcctttgggc attttcggca aagccgtcaa
    12961 agacgacacg ccgctgcgcg acggcgaccg catcgaagtg taccgcccgc tgttgatcga
    13021 ccccaaagaa gcgcgccgca aacgcgttca aaatcaagaa gaataaccat gccgtctgaa
    13081 gccttcagac agcattcgac agaaacacgg aaaatatcat gtcaaacaca atcaaaatgg
    13141 ttgtcggctt gggcaacccg ggcaaagaat acgaacagac acgccacaat gcgggttttt
    13201 ggttcctcga cgaactggcg tggaaatgga aggcttcatt taaagaagaa aaaaaattct
    13261 tcggcgaagt cgcccgtgcc gccctgcccg acggcgacgt ttggctgctc aaacctgcca
    13321 cgttcatgaa ccgttccgga caggcagttg ccgcccttgc gcagttttat aaaatcaaac
    13381 ccgaagaaat cctcgtcgtc cacgacgaac tcgacattcc ctgcggacgg atcaaattca
    13441 aactcggcgg cggcaacggc ggacacaacg gcttgaaaga cattcaggca aaactcggca
    13501 cggcagacta ttaccgtctg cgcctcggca tcggccaccc gggcgaccgc aacctcgtcg
    13561 tcggctatgt cctgaacaaa cccagtacgg aacaccgccg acagattgac gatgccgtcg
    13621 ccaaatccct gcaggccata cccgacatcc ttgccggcaa atgggaagag gcaacccgct
    13681 tcctgcacag caaatgaccc gatgccgtct gaagcccttt cagacggcat gttcccgatt
    13741 tccatatccg aacagtcatg accgaactca agcagcttat ccaaaccgaa tccatccccg
    13801 tcatcgaaga aaccctcgat ttcctgctgt acgaatgcag catcgacgac gcaccatccg
    13861 ccgaagaagt ggcacaatgg cgcgacatac ttgccgcacg cggcggcaaa ttcctgcgcc
    13921 tgtccaaaat ctgccaaacg tggctggacg aggaggcggc atgaagctgc cgcgcaaccg
    13981 cttcagcctg ctttccgcat tgtggtttgc cggcggcatc tattcgctgc tcttcaaagc
    14041 tgccgacacc gcgccgccgc cgtttccgca tttcgacaaa gcagcacacc ttgccctgtt
    14101 tttcgcacaa atctggcttt tgaccaaagc attcaaaacc ggaaaacttc ccatccccta
    14161 ccgcagcctg atggtctttg ccctctgttt cgccctcttc agcgaatgcg cgcaggcatg
    14221 gtttaccgca acgagaaccg gcagtttggg cgatgtcctt gccgacctga cgggtgcatt
    14281 gctcgcactc tttgccgcac gttccgccga ccgcccggac tgaatcggtt tattttcccc
    14341 aaacaggatg cacctcttcc cgatacatcc tatttttccc cttacatatc aatctcatct
    14401 cataaacaaa accgacaaat cgtttacaat atatttacac tacatcagat tacaaatata
    14461 ctcgaaccag ttgcagaagc ggcgattcca caaaccgttt cggattgcac gacacaaaag
    14521 aaaaaccgat tttgttgcct taaaggagta ttcatgaacc tgcatgcaaa ggacaaaacc
    14581 cagcatcccg aaaacgtcga gctgctcagt gcgcagaagc cgattaccga ctttaagggc
    14641 ctgctgacca ccattatttc cgccgtcgtc tgtttcggca tttaccacat cctgccttac
    14701 agccccgatg ccaataaagg tatcgcgttg ctgattttcg ttgccgcact ttggtttacc
    14761 gaagccgtcc acattaccgt aaccgcactg atggtgccga ttctcgccgt cgtactcggt
    14821 ttccccgaca tggacatcaa aaaggcgatg gctgattttt ccaacccgat tatctacatt
    14881 tttttcggcg gcttcgcgct tgccaccgcc ctgcatatgc agcggctgga ccgtaaaatc
    14941 gccgtcgccc tgctgcggct gtcgcgcggc agtatgaagg ccgccgtgct gatgttgttc
    15001 ctcgttaccg cctttctgtc catgtggatc agcaacaccg ccaccgccgc gatgatgctg
    15061 cctctggcaa tgggtatgct gagccacctc gaccgggaaa aagaacgcaa aacctacgtc
    15121 ttcgtcctgc tcggcatcgc ctattgcgcc agcatcggcg gcttgggcac gctcgtcggc
    15181 tcgccgccca acctgattgc cgccaaagcc ctaaatctgg acttcgtcgg ctggatgaag
    15241 ctcggcctgc cgatgatgct gttgattctg cccttgatgc tgctctccct gtacgtcatc
    15301 ctcaaaccta atttgaacga acgcgtggaa atcaaagccg aatccatccc ttggacgctg
    15361 caccgcgtga tcgcgctgtt gattttcctt gccacagccg ccgcgtggat attcagctcc
    15421 aaaatcaaaa ccgccttcgg catttccaat cccgacaccg ttatcgccct gagtgccgcc
    15481 gtcgccgtcg tcgtcttcgg cgtggcgcaa tggaaggaag tcgcccgcaa taccgactgg
    15541 ggcgtgttga tgctcttcgg cggcggcatc agcctgagca cgctgttgaa aacatccggc
    15601 gcgtccgaag ccttgggaca gcaggttgcc gccacctttt ccagcgcgcc cgcatttttg
    15661 gtgatactca tcgtcgccgc cttcattatt tttctgaccg agttcaccag caacaccgcc
    15721 tccgccgcat tgcttgtacc gattttctcc ggcatcgcta tgcagatggg gctgcccgaa
    15781 caagtcttgg tattcgtcat cggcatcggc gcatcttgtg ccttcatgct gccggttgcc
    15841 acaccgccta acgcgattgt gttcggcacg ggcttaatca agcaacgcga aatgatgaat
    15901 gtcggcatac tgctgaacat cctctgcgta gtattggttg ctctgtgggc ttatgctgta
    15961 ctgatgtaaa ccatcgacct aaacaacaag accgtctgaa agaatatttt tcagacggcc
    16021 ttgaagtttg tcgttcagac acaatttgtc gaatcattca aaaccggatt ctaacgaaag
    16081 gaaacccatg attatcctgc acaccaacaa aggcgacatc aaaatcgaac tcgatttcga
    16141 caaagccccc gttaccgcca aaaacttcga gcaatacgtc aaagacggct tctacgacgg
    16201 cgtaatcttc caccgcgtca tcaaaggctt catgattcaa ggcggcggca tggatgaaaa
    16261 catgaacgaa aaagaaaccc gcgatccgat tcaaaacgaa gcgtccaacg gcctgcccaa
    16321 cgataaatac accatcgcca tggcacgcac ttccgacccc cattccgcca gcgcgcaatt
    16381 cttcatcaac actgccgaca acgctttcct gaacttccgt tctaaagagc tgtacggcaa
    16441 aaccgtcgtc caagactggg gctatgccgt attcggtaaa gtcgttgacg gttttgacgt
    16501 tgtcgatgcc atcgaaggcg tatccaccaa acgccacggc taccatgacg acgtaccgag
    16561 cgaacctgtc gtcatcatta aagccgaagc ggtataaacc ggcaatccga aagcagcctg
    16621 cacaaaggct gctttttttg tttcaggcgg cataaaatcc aagcacatat gccgtctgaa
    16681 cacctcccgc aagccttcgg acaatgttgc aacgtgttaa aatagcctcc tttcctgaaa
    16741 ccgaaacgag ggaaccccta tggcaaatat cgcccacgac attttcaaag cctacgacat
    16801 ccgaggcatc gtcggtaaaa ccctgaccga cgaagctgcc tatttaatcg gcaaagccat
    16861 tgccaccaaa gcctccgaaa aaggcattac ccgcatcgcc ctcggccgcg acggccgttt
    16921 gagcggcccc ggcctgatgg cacaaattca acacggtttt acagacagca gcatcgacgt
    16981 cctcaacgtc ggcatgattg ccactcctat gctctacttc gcagccatca acgaatgcgg
    17041 cggcagcggc gtgatgatta ccggcagcca caatccgccc gattacaacg gtttcaaaat
    17101 gatgctcggc ggcgacacgc tcgcaggcga agccattcaa gaacttttag ctattgttga
    17161 gaaagacggt tttgttgccg ccgacaaaca aggcagcgta accgaaaaag acatctccgg
    17221 cgaataccac aacaacatcg tcggccacat taagctcaaa cgtccgatga aaatcgtgat
    17281 tgacgcgggc aacggtgtcg gcggcgcgtt tgccggcaaa ctctacaaag gtttgggcaa
    17341 tgaagtggcc gaacttttct gcgaagtgga cggcaatttt cctaatcacc accctgatcc
    17401 ttccaaaccc aaaaacctgc aagatttgat tgccgcgctg aaaaacagcg atgccgaaat
    17461 cggcttggct tttgacggcg atgccgaccg cttgggcgtg gtcaccaaag acggcaacat
    17521 tatttatccc gaccgccaat taatgctgtt cgcccaagac gtgttgagcc gcaatcctaa
    17581 agcaaaagtg attttcgatg tcaaatccac ccgcctgctg accccgtgga ttaaagaaca
    17641 cggcggcgaa cctgtgatgg aaaaaaccgg ccacagcttt atcaaatccg ccatgaaaaa
    17701 aaccggcgcg ctggttgccg gcgaaatgag cggacacatc ttcttcaaag aacgctggtt
    17761 cggcttcgac gacggtctgt acgccggcgc acgcctcttg gaaatcctgt ctgcctccga
    17821 taatccgtcc gaagtgttaa acaacctgcc gcaaagcatt tccacgcccg aactcaacat
    17881 cgccctgccc gaaggcagca acggccatca ggttatcgac gaactcgccg ccaaagccga
    17941 atttgaaggc gcaaccgaaa tcatcaccat cgacggtctg cgcgttgaat ttcccgacgg
    18001 cttcggtctg atgcgtgctt ccaataccac gccgattctg gtgttgcgtt ttgaggcgga
    18061 tacgcaagaa gccatcgagc ggattcaaaa ccaattcaaa gccgtcatcg aaagcaatcc
    18121 gaatctaatc tggcctctgt aaacacagga aaaatgctta atcaaatctt ggtagattgg
    18181 atttggctta aaaatctccg cgtccgttcg tgtttggggt ggtcgaacaa ctctttcggg
    18241 ctgccctgct ctacgataac gccgccgtcc atcacgacaa cggtcgtggc aacttccagc
    18301 gcgaacttga tttcgtgggt aacgacgacc atcgtccaac cttcccgcgc caattccttc
    18361 atggcgttca acacgtcttg caccaattca gggtcgagtg cggaagtggg ttcgtcaaac
    18421 aacatcagct cgggctgaat cgccaatgcg cgggcaatgc cgacgcgctg ctgctgaccg
    18481 ccggaaagct ggtagggata caaatccact ttgtcgccca agccgacttt ttccagcagt
    18541 ttcagagcct cttcgcgcgc ttgggcggca ggcttgccct gtacggcaac cggtccttcc
    18601 attacgtttt ccaaggcggt tttgtgcgga aagaggttgt attgttgaaa caccatgcct
    18661 gatttgcggc gcagtgccaa aatatcgtgt ttgcttggtt ttttagaaaa atcgattttc
    18721 agcggtcgct cgttgtcgaa ctcgatttgt ccgtcttcgg gcatttccaa cgcgtttagg
    18781 catcgcagaa acgtcgtttt gcctgagccg gaaggcccga ggataacgac cacctgccct
    18841 ttgcccacat ccaaattgat gccgcgcaaa atagtgcttt cgccgaaggt cttatggata
    18901 ttgcggattt taatcataac aactccttat ttggcgacat aacggtcgaa acgtttttcc
    18961 aaacgtgcct gaatcaggaa cagaaccttg caaaaacacc agtaaaccaa agcggcttcg
    19021 atatagacgg gcaaaaagtc ataagtgcgg tttgccgttt cctgcgcgac gcggaataat
    19081 tccgttaccg tcacgactgc cgcgagcgag gtgtttttaa acaaaccgat aaactcgttg
    19141 ctcaaaggcg gcacggcaac gcgaaatgcc tgcggcgcga cgatgcggcg gaacgtctgc
    19201 atataggtca taccgatgga gaaacctgct tcccattggc ctttaggtac ggacaaaatt
    19261 gccgcgcgta tggtttcgga agcgtatgca ccgacattga gcgagaagcc gataatggcg
    19321 gcgggaatcg gatcgatata gataccgacg gaaggcagcc cgtaaaacac aatcacaagc
    19381 tgaaccaaca gcggcgtacc gcgaatgacg gaaatataaa attccaccaa tttcagcagg
    19441 attttccgca cgatgccgcc ggcgggcata atccgcacca aagccacggc taccgcaatc
    19501 atcataccga taacgaaaga agctgccgcc aaaggcagag agaccgcgaa gccggctttg
    19561 accataggca aaaacgcgct gacaatcata tcggcgcgtg tttccgtcat aaacggcagc
    19621 gaagcaagga aattattgaa cactgatgtc ttttccgaag aattgttcgc ccagtttttt
    19681 cagcgtaccg tcggctttca gctcgttgat tgccgtactg aatttcgcca cggcttcgtc
    19741 attgcccttg ttgacaatca ggccggaacc gactttttca tcggcaggtg cggaccaaac
    19801 gattttcacg cccgcattcg ggtttttctt cagatagtcc aaaaccgcca attcgtcgct
    19861 cagggttgca tcggcacgtt tttgttcaat cagggtcagc gattgcgcca aaccgtccac
    19921 tgccaccaaa tctgcgcctg cagctttggc tttttcgccg tagttgctgg tcagggattg
    19981 tgcggttttc acgcctttga tgtcgtcgat agatttgatg ttgctgtcgt tacgggcaac
    20041 caatacggca ccgctccagc tgtaaggatc ggatttgtcg aatgtcgctt ggcgttcggg
    20101 gctggtcaga ccgacttggt ttgccaccac gtcgaaacgc cccgccttca aacccgccat
    20161 catcgaatcc cattgcgttt ctttaaactc gactttcacg cccagttttt ccgccacggc
    20221 gcgggttact tccacatcgt aaccggtcag tttgccgtcc ttgtcgtggt aggtaaacgg
    20281 tgcgtaagtg ccttccgtgc cgacggtaac cgtgccttta ttgttgatgc gctcgattaa
    20341 agaaccggaa actgccgatt gtgcaggcgc ggaagatgct ccgctgccgc tttccgaacc
    20401 gccgcaggcc gccaaaacca atgcggcaat gccgccgagt acgaattttt tcaacatcat
    20461 tctctcctgt aaaaagtgtg caagtgcggc attctaagtg aaattgcggc aaacacaaag
    20521 aatacttttt ttatatggtt attccaaatc ggaatggaaa agatatgccg aaaccgccgg
    20581 aagcggaaat aaagggggcg ggaaaggcga cgggcggcac gaaaccgaaa agcggcatca
    20641 ggtatgcaaa cggcggattc acgcacgccg ttccggcaaa aatccgcctg ccgattcggt
    20701 gctataatcc atccgaaaca gatgacacca aaaaggaaca tctcatgaac acgccccaat
    20761 ccgccatcat tcccgaccac gcccaagccg gcatctttat cgaagccgac ttcgccgcca
    20821 accgcctcaa cgatattaaa gccgcctgcc gcgcttcgct cgacgcgtta actgccttga
    20881 aagcccgctt tccggacgat attttgggtc tgaccatcgc cttcggcagc aaagcctggg
    20941 caacattcgg acatacggac gaaggcagtg aaatcaaacc cttctccgaa atgggcaacg
    21001 ggcttgcgcc gtccacgcag cacgatatgt ccatccacat ccaatccttc cgccaaaacg
    21061 ccgcctacgc gcttgcccaa tccgttttag gcgcgttcgg cgacagcata tgcgtcgcgt
    21121 ccgaagaaca cggtttgcgc ctgtatcagg atcgcggact ggacggtttc gtcgacggca
    21181 cggaaaaccc gcagggcgat gaaaatgtcc gcgaagtcgc catcatcccc gaaggaaaac
    21241 ccgatgcggg cggcagctat gtcctgctgc aaaaatacct gcacgatttg aaaaaatggg
    21301 atgccgtccc cgtcgccgaa caggaggcct cggtcggacg cggcaaggaa accgacgacg
    21361 aattcggccg cgatgtccgc ctgcccgatt cgcacctcgg ccgcgtcaac ctgaaagaaa
    21421 acggagtcgg cctgaaaatc gtccgccgca gcctgccctt cggcaaaatc agcggcgaac
    21481 acggtttgat gtttaccgca tactgccgca cgctgcacaa tatcgaagcg caattattga
    21541 gtatgttcgg cgatacggac ggcaaaaccg acctgctgct ccgacacctc tccgccgccg
    21601 tttcgggcgg atactactac gcgccgtctg tcgaacgcct gcaaaacctc taagccggac
    21661 gatgccgtct gaaaccattt ttcagacggc atttt
//
LOCUS       DAWKST010000014        21695 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.014, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000014 DAWKST010000000
VERSION     DAWKST010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21695
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.014"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(70..1311)
                     /gene="odhB"
                     /locus_tag="WJL87_000366"
     CDS             complement(70..1311)
                     /gene="odhB"
                     /locus_tag="WJL87_000366"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ7798850.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAATAAAEAPAAATA
                     AAEAPAAAPAEAAPAAAPAAAQNNAAMPAAAKLAAESGVDVNALQGSGRDGRVLKEDV
                     QNAAAKPAAAAAPAVALPAGARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNM
                     KPIMDLRAKYKEKFEKEHGVKLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFD
                     IGIAIGSPRGLVVPILRDADQMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNG
                     GTFGSMMSTPIINPPQSAILGMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREA
                     VLTLVAIKDALEDPARLLLDL"
     gene            complement(1411..4239)
                     /locus_tag="WJL87_000367"
     CDS             complement(1411..4239)
                     /locus_tag="WJL87_000367"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ7798851.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETDLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVALCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(4444..5727)
                     /gene="gltA"
                     /locus_tag="WJL87_000368"
     CDS             complement(4444..5727)
                     /gene="gltA"
                     /locus_tag="WJL87_000368"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ7798852.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            complement(5839..6087)
                     /locus_tag="WJL87_000369"
     CDS             complement(5839..6087)
                     /locus_tag="WJL87_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ7798853.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(6091..6798)
                     /locus_tag="WJL87_000370"
     CDS             complement(6091..6798)
                     /locus_tag="WJL87_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ7798854.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(6919..8682)
                     /gene="sdhA"
                     /locus_tag="WJL87_000371"
     CDS             complement(6919..8682)
                     /gene="sdhA"
                     /locus_tag="WJL87_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ7798855.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDSQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(8685..9026)
                     /gene="sdhD"
                     /locus_tag="WJL87_000372"
     CDS             complement(8685..9026)
                     /gene="sdhD"
                     /locus_tag="WJL87_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ7798856.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(9020..9397)
                     /gene="sdhC"
                     /locus_tag="WJL87_000373"
     CDS             complement(9020..9397)
                     /gene="sdhC"
                     /locus_tag="WJL87_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ7798857.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(9676..11079)
                     /locus_tag="WJL87_000374"
     CDS             complement(9676..11079)
                     /locus_tag="WJL87_000374"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ7798858.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(11328..12065)
                     /locus_tag="WJL87_000375"
     CDS             complement(11328..12065)
                     /locus_tag="WJL87_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ7798859.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(12344..14620)
                     /gene="metE"
                     /locus_tag="WJL87_000376"
     CDS             complement(12344..14620)
                     /gene="metE"
                     /locus_tag="WJL87_000376"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ7798860.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADFVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGTVEFNRLSLLQKLLPVYVEILNALVEAGAEWIQIDEPALTVDLPKEWV
                     EAYKDVYATLSKVSAKILLSTYFGSVAEHAALLKSLPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGIIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTHKLRAELAK"
     gene            complement(14758..15636)
                     /gene="metF"
                     /locus_tag="WJL87_000377"
     CDS             complement(14758..15636)
                     /gene="metF"
                     /locus_tag="WJL87_000377"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ7798861.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            15836..16111
                     /locus_tag="WJL87_000378"
     CDS             15836..16111
                     /locus_tag="WJL87_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ7798862.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            16111..16236
                     /gene="ykgO"
                     /locus_tag="WJL87_000379"
     CDS             16111..16236
                     /gene="ykgO"
                     /locus_tag="WJL87_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ7798863.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
     gene            complement(16342..16542)
                     /locus_tag="WJL87_000380"
     CDS             complement(16342..16542)
                     /locus_tag="WJL87_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798864.1"
                     /translation="MIGRLFRIFFFFALAALIMNRLFSRRQKRALREVAEISARVLLG
                     AAAATLFWYLFMLYFKHIPDSY"
     gene            16685..17824
                     /locus_tag="WJL87_000381"
     CDS             16685..17824
                     /locus_tag="WJL87_000381"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ7798865.1"
                     /translation="MSQNASVGIVTPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPVVTVKDWVKSQAALADYLGIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            17821..18402
                     /gene="metW"
                     /locus_tag="WJL87_000382"
     CDS             17821..18402
                     /gene="metW"
                     /locus_tag="WJL87_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ7798866.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            18519..19364
                     /locus_tag="WJL87_000383"
     CDS             18519..19364
                     /locus_tag="WJL87_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ7798867.1"
                     /translation="MKNKTSSLLLWLAAMMLTACSPSKEDKTKENGASAASSTASAAS
                     SSAPQTDLQPAASAPDNVKQAESVPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(19414..19974)
                     /gene="efp"
                     /locus_tag="WJL87_000384"
     CDS             complement(19414..19974)
                     /gene="efp"
                     /locus_tag="WJL87_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ7798868.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKIEIDTRTGEFRKRA"
     gene            complement(20017..21165)
                     /gene="earP"
                     /locus_tag="WJL87_000385"
     CDS             complement(20017..21165)
                     /gene="earP"
                     /locus_tag="WJL87_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ7798869.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDVVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEA
                     VRFDSEALRQRLMLPEKNAPEWLLFGYRSDVWAKWLEMWRQAGSPMTLLLAGTQIIDS
                     LKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTPETVSAHRRLSDDLNGGGALSAT
                     QRLECWQILQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(21184..21462)
                     /locus_tag="WJL87_000386"
     CDS             complement(21184..21462)
                     /locus_tag="WJL87_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798870.1"
                     /translation="MSRNLLVRWLVVCLIPLATLAVFVANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIVLLVVYLFHYFGAF"
BASE COUNT         5350 a         5497 c         5492 g         5356 t
ORIGIN      
        1 tttaaattta atccactata aaaattcaga cggccttttc attaacaaat aaaaggccgt
       61 ctgaaacgat tacagatcca acaacaggcg ggctgggtct tccaacgcgt ctttaatggc
      121 taccaaggtc aatacagctt cgcggccgtc aatgatacgg tggtcgtaag acagagccag
      181 atacatcatt ggacggacaa caacttggcc gttttcaacc acagcgcgct ctttagtggc
      241 gtgcataccc aaaatcgcag attgaggcgg attgatgatc ggggtggaca tcatagaacc
      301 gaaagtaccg ccgttggtaa tactgaatgt accgccggtc agatcttcga tagcgatttt
      361 gccgtctttg gcttttttcg cgtaatcaac aattgcttgt tcgatgtcgg caatgctcat
      421 ttggtcggca tcgcgcagga ttggcacaac caaaccgcgt gggctgccaa tcgcgatacc
      481 gatgtcgaag tagccgtggt acacaatgtc tttgccgtca acagaagcat tcacaaccgg
      541 gtattttttc agggctgcaa cagcggcttt aacgaagaag gacataaagc ccagtttcac
      601 gccgtgttct ttctcgaatt tttctttgta cttcgcacgc aagtccatga ttggtttcat
      661 gttgacttcg ttgaatgtag tcagaatggc gttttcttgt tgagaagcca agaggcgttc
      721 tgcaacacgg gcacgcaggc ggctcatcgg tacgcgttct tcaggacgtg cgccggcagg
      781 aagtgcaacg gcaggagcag cggctgcggc aggtttggct gcggcatttt gtacgtcttc
      841 tttcaatacg cgaccgtcac ggccggaacc ttgcaatgcg ttcacgtcaa caccgctctc
      901 tgcagccagt ttggcggcgg caggcatagc ggcgttgttt tgtgcagcag caggagcggc
      961 ggccggggca gcttcggcag gagcggcggc aggtgcttca gcagcggcag tagcagcggc
     1021 aggtgcttca gcagcggcag tagcagcggc aggtgcttca gcagcggcag tagcagcggt
     1081 atcgatacgt gccaaaactt ggtcggcaac aacggtttca ccgtcttgcg ctacgatttc
     1141 aaccaatacg ccggcttgtg gagaaggtac ttccaaaacc actttgtccg tttcgatatc
     1201 gatcaggatt tcgtcacggg caactgcttc gccaactttt ttcttccatt ccaagagcgt
     1261 gccttcagat acgctttcag acagcatagg tacttttaca tcaataatca ttttgtgtct
     1321 ccaatggccc tttcagacgg cctgttgtgt gtttttcatt ctgcatatgg gtgttccgcc
     1381 tgagcagcct cagacggaat gctacttggc ttacaatgcc aaagcgtctt caaccaattg
     1441 tttcaattga gcaatgtgtt tgcttgagta gcccactgca ggcgatgcgc tgcttggacg
     1501 accggcataa gacagttttt gctcttcgct gataacgtct tcgatgcggt ggcggatttg
     1561 gtagaacgcg ccttggtttt tcggctcttc ttgtgcccaa accacagatt ttgcgttcgg
     1621 atatttcgcc agttcggctt taacctcgtc gtatgggaac ggatacagct gctcaacgcg
     1681 gacaatagcg acatcatctt ccagtttacg ttcggctcga ccggcttcca agtcatagta
     1741 aacctgaccg gcacacaatg ccacgcgttt cacgctgtcg ttgcttgcgc gttcggcggt
     1801 atcgccgata accggacgga aagtcgaacc ttcggtgaag ttttccagcg ggctcattgc
     1861 ccctttgaat cgcaacaggc gtttggacat gaaaatcact agcggtttgc ggtatgaacc
     1921 taagacttga cgttgcaaga ggtggaacat ttgcgacgct tcagacggca tgatgacttg
     1981 catattgttc tcagaacaca gttgcaacca acgttctacg cgtgcagaag agtgctcggg
     2041 accttgaccg tcgtagccgt gcggaaggat ggtggtcaga ccgcacaaac gaccccattt
     2101 ggtttcgcct gaagacagga attggtcaat agtcacttgc gcgccgttgg cgaagtcacc
     2161 aaattgagct tcccaaatgg tcagcttgtc aggcgcggag caggcaaagc cgtactcgaa
     2221 cgccatcacg gcttcttcgt tcaaaatgga gtcgataacc aagaactcgc ccataccttc
     2281 gcccatatgg cgcagaggaa cataagtacc gtcatcccat ttttcacgtt tttgatcgtg
     2341 caatactgca tgacggtgtg agaatgtacc gcgcccggag tcctcaccgg agatacgcac
     2401 gccatgacct ttggttacaa ggcttgcgta tgccaaggtt tcggccatac cccagtcgat
     2461 ggcttgtttg ccggatgcca tggctttacg cgcttcaatc acacgttttg cagtcggatg
     2521 cagggcaaag ccttccggta cggcggtaaa tttctcggta agacgctcaa tatccgcggc
     2581 aggcaaatcg gtttcgatgt gttcgcgcca atctttacct tggtatttgc tccagtcaat
     2641 ttgtgtgcgt tggaagttgc tcaacgttgt ttgctcgacg tgttcgcctt tatccaaagc
     2701 atcacggtag gcttggatgt aaccgtcagc ctcggcttgg gttaccacgc cttcggcaat
     2761 cagttgctcg gtgtacaaag cacgcgcacc cgggtgttgc gatacttttt tgtacatcat
     2821 cggttgggtc aaggtcggat cgtcgccctc gttgtggccc catttacggt agcagacaac
     2881 gtcgatcacg atgtctttat ggaatttttt gcggtaatcc aaagcggctt ggatggcaaa
     2941 gcaaacacgc tcaggatcat cgccgttcac atggataacc ggagcggaaa ccatttttgc
     3001 gatatcggta cagtgtacgg ttgaacgggt atcgcggata tcggaagtgg taaagccgat
     3061 ttgattgttg atgacgatat gaaccgtacc gccggtggta taaccgcgcg ttttagacag
     3121 attgaatgtc gcttggttga cacccaaacc gataaatgcg gagtcgccgt gaatcaatac
     3181 gggcaagact ttatcgcggc cgttttcgcc caaacgtttc tgcttggcgc gtgcagaacc
     3241 ttcgactacc gggttaacga tttccaagtg tgacgggttg aacgccaaag aaacgtgcat
     3301 cggaccatgt ggagtggcga tgtcggagct gaagcccatg tggtatttca cgtcgccgct
     3361 aggcaatttg atttcggcac ggccttcaaa ctcagcgaac aaatctccgg gttttttacc
     3421 caaaatgttc accaaaacat tcagacggcc acggtgcgcc ataccgatga tgacttcttc
     3481 cacgccgtct ttaccggcgt tttgaatcag gtagttcaaa ccggcaatcg cgctttcgcc
     3541 gccttcgaca ccgaaacgtt tctgaccgac atatttggta tgcagataac gttccaaagt
     3601 ctcggcagca gtcatctctt tcaagatacg gcgtttttga tcggcattgt aatgcggtgt
     3661 ggacaatacg ctttcaaaat aattgcgtac ccagcggcgc tcttcggtat tgggaatata
     3721 gatatattcc aatgcgatgt ggccgcagta ggtttgtttg aggttgctga tgatttggga
     3781 caaaggcagt ttgccgcgat tggcaaaatc gccctcgccc atattgaatt gaagcgccat
     3841 atcggcatct gacagaccgt ggaatttcgg atcgagggct tcaatatcgc gcggggggat
     3901 acgtttgagc ggatcaagtt gggctgcacc cacgccttgg atacgatagg cggaaatcag
     3961 ccgtaaaacg ctgacttgct ttttcagcat tgcctcatcc gcaccgcccg caacggcaga
     4021 tgcaattttc tttttcgcca aagtaacaaa tgattcgcga atcggtgtgt gtgcgacatc
     4081 gacagcaacc gtccccggct gtttgctcaa atcggtgaaa tactgcttcc atttttcatc
     4141 aaccgcatcg gggttttcca aaaaagcctc gtacaattcc tcaatgtaag gtgcgtttga
     4201 accgaacagg taagagaaat tgagtttttc gtccatcatg gcgtgcgctc ttttctttaa
     4261 aatataaaca aagggtagaa gtccgcgtcc tgacggctaa actgatgccg cctgaaaaga
     4321 cattttattc taccctattt taaaccggat tgcttgtatt gatttcatcc gactacaatc
     4381 agacggattc gggaaacaat ccggttctct gatattgcct gtttgacaat atattaatgt
     4441 atgttacctc tcgcctggcg gcacataatc gcggcgttcc gaaccggtat aaagctggcg
     4501 cgggcggccg attttcagcg aaggatcgct aatcatctcg tgccagtgcg aaatccagcc
     4561 cacgctgcgc gacagggcga agatgacggt aaacatttcg gtcgggatgc ccagcgcgga
     4621 caggacgatg ccggaataga aatcgacgtt tggatacagt ttgcgttcga taaagaacgg
     4681 gtctttcagc gcaatctgtt ccaattccat cgccagtttg aatttcggac tgtcttccaa
     4741 gcccaattcc ttcaaaactt catagcaggt ttcacgcata atgctggcac gcggatccat
     4801 attgcggtac acgcggtgac cgaagcccat cagacggtat ttgcgttgtt tcacaccttc
     4861 catgtatgcg gcaacattag acacatcgcc gatttcgtcc aacattttca acacggcttc
     4921 gttcgcaccg ccgtgcgaag caccccacag gcaggcgata ccggcagcaa tacaggcaaa
     4981 cgggttcgca cccgaagacc ctgccagacg gacggttgaa gttgaggcgt tttgctcgtg
     5041 gtcggcatgc aaaataaaga tgcggtcgag cgcgcgtgcc aaaacgggat tgggtttgta
     5101 gtcttcacac ggcgtggcga acatcatatg aaggaagttt tcggaataag aaagattatt
     5161 cttcggataa ttgaacggca gaccgtttga atagcggtag cacattgccg caatggtcgg
     5221 gattttagaa atcaggcggt aaatcgcgat tttgcggtgt tcgggattgc taatgtccaa
     5281 gctgtcttgg tagaacgcag acagtgcgcc gaccacgccg accatcatcg ccatcggatg
     5341 cgcgtcgcgg cggaaccccc ggaagaacca agtcagctgt tcatgcacca tcgtgtggcg
     5401 gcggacggta ttgtcaaatt ctgccttttg ctcgggagtc ggcagttcgc cgtaaatcaa
     5461 caggtagcag acttccaaat aatcggactt ttcggccagc tgctcgatgg ggtatccgcg
     5521 ataataaagc aagccttgat cgccgtcgat gtaagtaatt ttagactcac agcttgcggt
     5581 tgaaacaaat ccggggtcga aggaaaacaa acctgtattt tttgtcagcc cccgaatgtc
     5641 aaccacatcg tgcccgatgc tggcttccaa taccggcagc tccaaacctg cctgacccgg
     5701 tacgttgagt ttgattgatt tggacatatt attgctcctt tgcaagttat aacccacgtt
     5761 actgcatccg cagcacgttc tttgccttca gacggctttt gcattttgaa atctgcctgc
     5821 aggcagattt cagatgcctc atgcccgtct gattttttca agcatcggga taaggtgccc
     5881 tttgtccgtt tccgaatgcc cgttaatcaa ggcaagcaat tcttgatctt gaaattcaag
     5941 gatttcggaa aactcggaca gctctttatc gctcaaatgc tcgaattctt tttccataaa
     6001 cctgccgaag attaaatcta attccaacaa tccccggcgg gtttgaaaac ggattttccg
     6061 tttggcaata tcgtcaaaaa ccatcatttc ttaaacggcc cgtttcaaca taatctcttt
     6121 aatcttaccg atggctcggg tcggattcaa gtgtttagga catacgtcta cgcagttcat
     6181 aatggtgtgg caacggaaca aacggtatgg gtcgttcaga ttatccaaac gctcattagt
     6241 gatggtatca cggctgtccg caatgaaacg gtaagcattc agcaaaccgg acggaccaac
     6301 gaatttatca gggttccacc aaaatgacgg gcaggcagtc gaacagcagg cgcacaaaat
     6361 acactcgtac aaaccgtcca actctttacg ctcttcctga gtttgcagac gctctttgtc
     6421 cgcatcaatc ggattatcgt tgacaacata aggtttgacg gaatggtatt gtttgaagaa
     6481 ctgggtcata tccacaatca ggtcgcggat aacaggcaga cctggcagag gacggatttt
     6541 aaccggctgt ttcaagccac gcagatcggt caaacacgcc aagccgtttt tgccgttgat
     6601 gttcataccg tccgatccgc aaatgccttc gcggcaggag cggcggaaag acaaggtatc
     6661 gtcttgtgct ttcaggcgta ccaaagcatc caaaagtttc acgtcggtcg gttccaattc
     6721 caactcgtaa cgctgcatat aaggcttggc atcaacatcc gggttgtaac ggtaaatttc
     6781 aaaactcatt ttttccatga gaagtgttcc ttttctcatc aactgcccaa tgattttgta
     6841 ttataaattc aagcagttca atgtgggtat ggttagattt cagacgagct tatgaggcga
     6901 agaccgtctg aaaacgcatc aataaacgcg cttggccggt ttgatgtatt ccacgctcaa
     6961 aggcttggta tgtaccggtt tgtaagacaa agtattggta tctgaatggt acagcgtatg
     7021 tttcatccag ttttcatcat cgcgctcagg atggtcgtct gaagcgtgcg caccgcgtga
     7081 ttctttacgt gcttcggcag acaccaaagt cgctttcgcc acttcaatta ggttatccaa
     7141 ttccaaagcc tcgatacgcg cggtattcca cactttgctc ttgtctttga tttcggtacg
     7201 tttcacacgc tcggcaatcg ccatgacttc tcgaacgcct ttgctcagaa tctcatcagt
     7261 acggaacacg ccggcgtgca attgtacgga gcgttgcagt tcgcggcgca atgcatcaac
     7321 gttttcacca tcggtttggc tgtccaaacg ctcgatacgt tggcgggtca actcaccagc
     7381 attagcaggc aaaggtttcc agtcgctttg ctctttgatg aatttaatca tgctgtcgcc
     7441 ggcagcttta ccgaatacca ccaagtccaa cagggagttg gtacccaagc ggttcgcacc
     7501 gtgtacggaa gcacaagcgc actcacctgc cgcatacaga ccttttacag gcacttcgta
     7561 ttcgtcgcct tgaggaacga caacttcgcc atggtagttg gtcggaatac cgcccatcat
     7621 atagtgggta gtcggcacaa cgggaatcgg gtctttaatc ggatcgatac cggcgaactg
     7681 aatggaaatc tcgcggatgc ccggcagttt ttccataatt ttttctgcgc cgatatggtc
     7741 gattttcagt aagacatggt ctttgttttt accgcagccg cgaccttcgt agatttccat
     7801 cgccatcgcg cgggaaacaa cgtcgcgaga agccaagtct tttacggtcg gcgcatagcg
     7861 ttccataaag cgttcgccgt cggcattcaa cagaataccg ccctcgccgc gtacgccttc
     7921 ggtaatcaac acgcccgcac ctgccacgcc ggtcgggtgg aattgccaga attccatatc
     7981 ttccaacgga atacccgcac gcgcgcaaat gcccaaacca tcgccggtat tcatataggc
     8041 attggtagaa gaagcataaa tacgaccgcc accgccggta gcaaacatca cagctttagc
     8101 gtggaaaata taaacttcgc cggtttccat ttccatggcg gttacgccga cgacatcgcc
     8161 gttttcatca cgaatcaaat cttgtgccgt ccattccaca aagaattgcg tattggcacg
     8221 gacgttttgt tggtacaaag tatgcagcat cgcatgacct gtacggtcgg caaccgcaca
     8281 ggcgcgttct accgcgcgtt taccgtgttc ggcagtatgg ccgccgaaag gacgctgata
     8341 aattttaccg ctttccacac ggtcaaaagg catacccatg tgttccaact caattacggc
     8401 ttcaggcgcg gcgcggcaca taaactcaat cgcatcttgg tcgcccaacc agtcggaacc
     8461 tttcacggta tcgtacatgt gccagtccca acggtcttcc tgcacattac ccagagaggc
     8521 ggaaataccg ccctgcgccg ctacggtatg cgaacgggtc gggaacactt tagacaaaac
     8581 ggcacaattc agaccggatt tggataattg gagggctgcg cgtaaacctg caccaccacc
     8641 gccgacaatc acggcatcaa acttgcgaac aggaaaaccc atacttaccc ccaaattact
     8701 ttaattgaat acaccaagca gccgaccagc cagacgatgg tggcaacctg caaaaacaaa
     8761 cgcacgccga agggtttgat atagtccatc cacaaatcgc ggatacccac ccaagcgtgc
     8821 aagaatacgg cgatgaagct cacttgggta aatactttta cccaagtttg actaaaaaat
     8881 gcctgccatg ccgaatattc tttaggcagg gaaaatagaa ccactaaaag tgcaacggta
     8941 taaatcaaca taataaccgc agtcgcacgt tgcatcgccc aatcgcgcaa accgtaatgg
     9001 gcaccggtca atttacgttc taccataaca acgctcccaa aacgacagtc aaaaccaatg
     9061 cagaagcaaa tacggcttta gcggtattgc gcgcagtatt cagctcaagg cctttgtgcg
     9121 catccaaaaa taaaaagcgg ataccggcga gagaatggtg cagataagcc cacaatacac
     9181 cgattaaaac cagcttgacc aaaggatggg aaacaatggc acggtaagtt tcaaatgcag
     9241 actcttgact cagggtaccg gacaggaaat acagcaggaa aggcagcata ataaacagcc
     9301 cgaccccgct gatgcggtga aggatggaaa ctatcccggg tatcggcaga cggatgttcg
     9361 gcaaatccag ataaacagga cgcggtttgg cagacatagt caattcctct gtagttatat
     9421 atcgactcaa aaaccgtaaa cccattgcta cactgggtta cataaacgtt aatttacctg
     9481 ttttatccca ttttgaacag tgtattttaa aaacaaactg atagattttg acaaacacgc
     9541 gtggaaacag cagattaaaa gaaagatttt tagttttgcc gcccccttcc cgacacggta
     9601 tcagcatccg accccatccc caaaaacaaa atgccgtctg aaaaaatttc agacggcatt
     9661 gttttgccat atcggtcagg ctttcaattt cgcatcggca tcgcgcaacg cggtacgcag
     9721 accttcctcg ataacgggat ggtagaacgg catatccagc atttgcggaa cggtcatctt
     9781 catttgatgc gcccatgcca gcaagtgcgc caaatgttcg gcggcagggc ctacgatttc
     9841 cgcgccgata aagcggccgg tggctttttc ggcatacagg cgcatatggc ctttgttcac
     9901 cagcatcacg cggctgcggc cttggttttt gaacgatact tcgccgatga caaattcgtc
     9961 ggcttggtat tgcgcggcaa cctgcgcgta tttcagaccg acaaagccga tttgcggact
    10021 ggtaaacacc acgccgatgg tgctgcgccg caaaccgccg ccgatattcg ggtagcggcc
    10081 cgcgttatcg ccggcaatct tgccttggtc ggcagcttca tgcagcagag gcagttggtt
    10141 ggacgcgtcg cccgcaataa agatatgcgg aatgctggtc tgcatggtca gtgggtcggc
    10201 aacgggtacg ccgcgcgcgt ctttttcgat attgatgttt tccaaaccga tattgtcaac
    10261 gttcggacgg cggcccacgg ctgccagcat atattcggca acaaatacgc ctttttcgcc
    10321 gtcctgctcc caatggactt ctacattgcc gtctgcatct agtttgacct cggttttagc
    10381 atccagatgc agtttcaatt cttcgccgaa cacggctttc gcctcgtctg aaacgacggg
    10441 gtcggaaatg ccgccgatga ttccgcccaa accgaaaatt tcaactttca cgcccaaacg
    10501 gtgcaatgcc tgacccagtt ccaaaccgat aacacccggc ccgaacacgg caacgcgctt
    10561 aggcagcgta tcccatgaga aaacgtcatc gttgataatc aaacgattgc ccaaagactg
    10621 ccattgcggc aggatgacgg gacgcgaacc ggtagcaatc acgaaacttt ttgccgtaat
    10681 ttgagtatgc tcgtcgattt ggacggtatg ctcgtcgata aatttagccg aacccatgat
    10741 gcgcttgtcg gcaggccact cttccacatc ggcaacgaca aagccgacaa aacggtcacg
    10801 ctcggattta acgcgctgca tgacctcttc accgttgacg acgatgctgt ctttgtccaa
    10861 atgcacgccg aacgggtcgg tatgcaatgc gtggtggcgc gcttctgcgg cggcaatcaa
    10921 gagtttggaa ggcatacaac ccacgcgcgc gcaggttgtg ccgaacacat tgttttcaat
    10981 caggtaaaca ttatccgaat gtaaacgggc attgcgaaac gcacccatac cggcagtacc
    11041 gccgccgatt acgacgacat ccgcttgaat ttttttcata atctttgtcc ttccgttttc
    11101 gtctgaaaca ttgccgacac agaacatatc ggtttcagac ggcatttaaa tattttccca
    11161 atcaaaaaca tcattcagat aaataaaaac aaaatttcga ttgagtgcaa caatatccaa
    11221 attctcttat aaatatagag ttattcggat cggcagtgta gaaccgcccg aataactcta
    11281 tccggtatat cagacagttt aatcaaaccg ccttaagcaa acagctatca gtttttagcc
    11341 aagtaagctt ccaaatcttc gctgccgccg atgtatttgc cgccgatgaa gacttgaggg
    11401 gcagtcatct tgccggtaat ggcgcgaacg gaagtaacgg ttgcatcttt gcccaatacg
    11461 atttcttcgt aagacaaacc tttgtcttgc aaagcttgtt tggctttggc gcagaattgg
    11521 caacctggtt tagtgaaaat tgccacagac tcttgagctt tccaatcagg agcaacgaat
    11581 ttcagcatag tatctgcatc ggatactttg aacggatcgc ctggttcttc aggctcgatg
    11641 aacatttttt caaccacgcc gtcgttaacc agcatggagt aacgccaaga gcgtttaccg
    11701 aagcccaagt cttctttacc gaccagcata cccatacctt cggtaaattc gccgttgcca
    11761 tcaggaatca tgtagatgtt gtcggattct tcttcggcag cccaagcgtt cattacgaac
    11821 gtatcgttta cagatacgca gtagattgcg tcaacgccgt tttctttgaa cgcgccgaac
    11881 aattcgttgt aacgcggcag gtgtgaagaa gaacaagtcg gggtaaatgc accgggcagg
    11941 gagaatacga ctactttttt gcctttgaac aaatcatcgg tagacacatc tttccaagtg
    12001 tcgccgacgc gggtgcggaa tactacggaa ggtacttttt gaccggtacg atcttgcaaa
    12061 gccattttaa gctccttaat agattggttt gagaaataat tgtgaatcgc tgggcattct
    12121 accccatgta aaatgatttg tataatttat agttcaaata atataaatta tttaaaacta
    12181 tcaaacacaa ctccgatttg cctgtttccc acatataaag ccgaattaga aaaaaacaag
    12241 ctgcaatgaa aagattttcc ggcaactgcg cccttgcgcc gcaaatcagg acaaaatacc
    12301 gtctgaaagg ctttcagacg gtatttattc atacggtctc ggcttatttc gccaattcgg
    12361 cacgcaattt gtgggttacg ttcatcatca cttggagttg ttccagagtt tctttccagc
    12421 cgcgtgtttt caggccgcag tccgggttaa cccacagacg ttcaaccggt acaacctcga
    12481 tggctttgcg caacaggtgc tccacttcgg cttctgtcgg tacgcgcggg ctgtggatgt
    12541 cgtaaacccc cgggccgatg tcgttcgggt atttgaattc gccgaacgcg gtcaagagtt
    12601 ccatgtcgga acgtgaagtc tcgatggtga tcacgtccgc atccattgca gcaatcgcag
    12661 gcaggatatc gttgaactcg gagtagcaca tatgagtgtg gatttgtgtg ctgtcttcgc
    12721 aaccggcaga ggacaggcgg aaggattcgc ccgcccagtt caggtaggca tcccaatcgg
    12781 cgcgtttcag cggcaagcct tcgcggatgg caggttcgtc aatttggatg actttgatgc
    12841 ctgctttttc cagatccaat acttcgtcgt tcagagccag tgcgatttgt ttgcacacgg
    12901 tagagcgagg aatatcgttg cggacgaaag accattgcag aatggttaca gggccggtca
    12961 acataccttt catcggacgt ttggtcaggc tttgagcgta agtagaccaa gcgacagtca
    13021 tggcttcagg acggcttacg tcaccgaaga taatcggtgg tttaacacaa cgtgagccgt
    13081 agctttgtac ccagccgtat tgggtgaatg caaaaccgct caacaattcg ccgaagtatt
    13141 cgaccatgtc gttacgctcg gcttcgccgt gtaccagtac gtccaaatcc agtttttctt
    13201 gttcttcaac caccaaggcg atttcttttt tcatcgcggc ttcgtaatcg gcggcagaca
    13261 gttcgccttt tttgaaggct gcgcgtgctt ggcggatttc ggtagtttgc gggaaagaac
    13321 cgatgttggt cgtcggcagc agaggcaggt tcaaccatgc ttgttgcgct ttgatacggt
    13381 cggcaaatgg agatttgcgt tggtctgcgt tggcaggcaa atcggccagg cgtttggcaa
    13441 cgtctgcacg gtggatttcg ctgctgttgg cgcgtgaatc ggcagcagct tggctggcgg
    13501 ccagttcttc ggcaacggaa tcgcgacctt cgttcaatgc ggctttcaga acgcgcaatt
    13561 cttgggtttt ttgcagggta aatgccaacc aagagtacag gtccggtttg ttggctttca
    13621 gtttttcttc aactgacaag tcaaatggag tgtgcagcag agagcaagag ctggaaatcc
    13681 acaaacggtc gcccagtttg gcttgcagag gctcaacagt ttccaaaact ttgttcaggt
    13741 tggcgcgcca gatgttgcgg ccgtcgatga tgcctgcaga cagaactttg tcgtagtcgg
    13801 caaatgcgtc cagttgctcg ggagcgcgta ccaagtcgat gtgcaggccg tcaacaggca
    13861 gggatttcaa caatgcggcg tgttcggcaa cagaaccgaa gtaagtgctc agcaggattt
    13921 tggcacttac tttgctcaaa gtagcgtaaa cgtctttgta ggcttctacc cattctttag
    13981 gcagatcgac agtcaaagca ggctcgtcga tttgaatcca ttctgcaccg gcttcaacca
    14041 aagcgtttag gatttcaacg taaacaggca gcagtttttg caacaggctc aggcggttga
    14101 attcgactgt gcctttttct ttacccaccc acaggaaagt caacggacct acaacggtcg
    14161 gtttggcttt cagaccgagg gcttgggctt cttgcagttg ttgaacatag tgtttggcat
    14221 tggctttgaa ttcggtatcg gcgtggaatt caggcaccaa gtagtggtag ttggtgtcga
    14281 accatttggt catttcgata gcgaattggt ctttgttgcc gcgcgccagt tggaagaatt
    14341 gttccaaaga caggttttgg ctgtcgaaac cgaagcgggc gggaatcgcg ccggtggcga
    14401 cttgcaggtc gaggatgtgg tcgtagaaag tgaaatcgcc tacggcaacg aaatcggcgt
    14461 tggcagcggc ctggtgtttc cagtttttct cgcgcaagtc tttagcaaca gccagcaatt
    14521 cttgctcgct gatttctttg cgccagtatt tttcttgtgc gaatttcaat tcgcggaagg
    14581 cgccgacacg cgggaagcct gagaaatgta atgttgtcat gttgaactct ccagttggat
    14641 tttgtaagga ccggttcgat ccaaatcggc cggtattgta aatcagaatg gctcggatgg
    14701 ggcggtgttt tgctttttag acaccttaga tgccgtctga aactgtttga tacggcttta
    14761 agggcgcacg cctaaaatat ggcagatggc gtaagtcagc tcgctgcggt taagcgtata
    14821 gaagtggaaa tctttcacgc cttcgcggga caggactttg accatatcga tggcgatact
    14881 tgccgccacc agattgcgcg taccttggtc gtcgtccaaa ccttcataca tttgcgacag
    14941 ccagcttggg attttgacgt tggttacttg cgccattttg ccgagctgct tgaagttggt
    15001 aacaggcaaa ataccgggga cgatttccac atcgataccc aacatcacgc agcggtcgcg
    15061 gaagcgcagg tagcgttcca catcgaagaa gaattgggtg atgacgtggt tcgcgcccgc
    15121 atcgattttg cgtttcagat tgatcaagtc ggcttgagcg gatttggctt ccgggtgtac
    15181 ctcaggatag gccgctacgg aaatgtcgaa gtcggcaacg gagcgcaaca gtttgaccaa
    15241 atcttcggca tagaaaggtt ttttctcgta gccgggcggc tcgtcgccgc gcagggcgac
    15301 aatgcggcgg atgccgctgt cccaatagtc tttggcgatt tggcgcaatt cgtcgggaga
    15361 agcgtcaata ccggtcaggt gcggggcggc ttccaagccg gtttcctgtt tgatgcgttt
    15421 gacgatgccg tgtgtgcggt cgcgctcgcc tgagtttgca ccgtaagtta cggaaacaaa
    15481 tttcgggtgc aaggtttgca ggcgatggat ggaatcccac agcatggttt ccatttgttc
    15541 gtttttcggc gggaaaaatt cgaatgaaac gttgatgtcg cctttcaaat cggaaaggct
    15601 gttatttaac gcattgattt cttttgcgta attcatggct gcgtaccttt tattatcttt
    15661 tatatgatgt ttcaggcaac ataataaagt tgcgccaaac atgagtcaat ttcaaaattt
    15721 tcagatttat catgaaaatt tctaatatat tacaaaaata gttaaatttc cttgtacagc
    15781 ccttctaaac tatgctagag tttgttatgt tataacactg aaagcaaggc aatatatgaa
    15841 accgaatatt catcccgaca attaccgaac cgtccttttt ttcgatagca gcgccaatga
    15901 aggctggtta atccgctcct gtgccgaaac acacggaaaa acgatggttt ggacagacgg
    15961 caaagaatat ccgctgtttt cgctggatac ttcttccgca tcccatcccg tctatactgg
    16021 caaacagcgc aacgtcaaca ccgaaggccg tgccagcaaa ttcaaccaac gtttccaatc
    16081 cgtaatgtca tcgtttagaa aggacaaata atgcaggttc tgtcttcact caaaaccgcc
    16141 aaacaacgcc accgcgactg ccaaatcgtc aggcgcaggg gcaaagttta cgtcatctgc
    16201 aaaagcaatc cgcgttttaa agcgcgccaa cgctgacccc ctgcccttcc tgcttcggtt
    16261 gtaaatcccc tcctaccgca agcccttttc aaaaaatgcc gtctgaaaca gaaaaatgcg
    16321 tttcagacgg cattttttcc gtcaatacga atccggaatg tgtttgaaat acagcataaa
    16381 cagataccaa aacagcgtcg cggcggccgc accgagcagc acccgggcac tgatttcggc
    16441 aacttcgcgc agggcgcgtt tctgcctgcg gctgaagagg cggttcataa tcagcgcggc
    16501 aagtgcgaaa aagaaaaaaa tacggaaaag tctgcctatc atgtcgggaa gcgaaaagta
    16561 tggttcgggc gcggcagcat ggggaaggat gtttcagcgc gtcaggctgc ttgtccgacc
    16621 gtgtcaaaaa gtcatgccgt ttgcgatata ataacgcttt ttgccgtatt tgttggaaca
    16681 ggatatgagt caaaatgcct cggtgggcat tgtaacgccc caaaaaattc cgtttgaaat
    16741 gccgctggtt ttggaaaacg gtaaaacttt gccgcgtttc gatctgatga ttgaaaccta
    16801 cggcgagctg aatgccgaaa aaaataatgc ggttttaatc tgtcatgcgc tgtcaggcaa
    16861 ccatcatgtt gcgggcaggc attcggcgga ggataaatat acgggctggt gggacaatat
    16921 ggtaggaccc ggcaaaccga ttgatacgga acgttttttc gtggtcggtt tgaacaatct
    16981 gggcggctgt gacggcagca gcggaccttt gtcgatcaat cctgaaacgg gtagggaata
    17041 cggcgcggat tttccggtgg ttacggtgaa ggactgggta aaatcacaag ccgcgcttgc
    17101 cgactatctc ggcatcgggc aatgggcggc ggttgtcggc ggcagtttgg gcggtatgca
    17161 ggctttgcag tgggcgattt cctatcccga acgcgtgcgc catgccttag tgattgcgtc
    17221 tgcgccgaaa ctgtccacgc aaaatatcgc gttcaacgat gtggcgcgtc aggcgatttt
    17281 gaccgacccc gatttcaacg aaggacatta ccgcagccat aataccgttc ctgcacgggg
    17341 cttgcggatt gcccgcatga tggggcacat tacctatctt gccgaagacg gtttgggcaa
    17401 aaaattcgga cgcgatttgc gttccaacgg ctatcaatac ggttatggcg ttgaatttga
    17461 agtagaatcc tatctgcgct atcagggcga caaattcgtc gggcggtttg atgccaacac
    17521 ctatttgctg atgaccaaag ctttggacta tttcgatccg gcggcggatt tcggcaacag
    17581 cctgacccgc gccgtgcagg atgtgcaggc aaaattcttt gtcgccagct tcagcaccga
    17641 ttggcgtttc gcgcccgaac gttcgcacga actggtcaag gccctgattg ccgcccaaaa
    17701 atccgtgcag tatatcgaag tcaaatccgc acacgggcac gatgcctttt taatggaaga
    17761 cgaagcctat atgcgtgcgg tcgccgccta tatgaacaac gtttataagg aatgtcagca
    17821 atgaacctgc gcgatgattt gcaactgatt tacgaccgga tacccgaagg cagccgcgtc
    17881 ttggacttgg gctgcggcga cggcgaactg cttgccgcat tggtcgaaca caaaaaatgc
    17941 agcggctacg gcatcgaaat cgacaccgac agcgtgattg ccgcgatgtc gcgcggcgtg
    18001 aatgttatcc aagccgattt ggaagaaggt ttaaccgcat tcaacgatca aagttttgat
    18061 gtgattgtgt tgagccaaac catccaagcg atgcagaata ccgaaaaaat cctgcgctgc
    18121 ctgatgcgcg ttgccaaaca ggcgattgtc agcttcccga atttcggcta ctggcgcaac
    18181 cgcgtccaaa tcgcactcgg cggacatatg ccggtttccg aacgtatgcc ctaccattgg
    18241 tacgacacgc ccaatatcca ttggtgcacg ctcaaagact ttgatttgtt gtgcgccaaa
    18301 aacaacatcc gcgtcctcga acgcgccgtg atgacgggca acagacaggt caaacatttc
    18361 cccaaccttt tgggcagcct tgcgttttac cgcgtcggct aagacaggtt gagaaccttg
    18421 tcggcaataa caggttcttc ggttctatta acaacaggcc gtctgaaaaa tgaaacggta
    18481 ggttttcaga cggcctcttt aatactggaa acaatagaat gaaaaacaaa acctcatcac
    18541 ttctcttatg gcttgccgca atgatgctga ccgcgtgttc cccgagcaaa gaagataaaa
    18601 cgaaagaaaa cggcgcatcc gccgcctcgt ccacggcatc cgccgcttcg tcttccgcgc
    18661 cccaaaccga tttgcaaccg gccgcatccg cccctgataa cgtcaagcag gcagaaagcg
    18721 tgccgccgtc aaattgcacc gacctgcacc ccgccaccgg cattgacgat ctcatgcagc
    18781 aaatcgccga acacattgac tcggactgtc tgtttgccct ttcccatcac gaactggaaa
    18841 cccgtttcgg cttacccggc ggcggctatg acaacataca gcggctgctg tttcccgaca
    18901 tccgccctga agatcccgac taccatcaga aaatcatact ggcaattgaa gacttgcgtt
    18961 acggaaagcg cacgatcagc cggcaggcac aagatgcctt gatggaacag gaacgccgcc
    19021 tccgagaagc gacgctgttg ctgatacagg gcagtcaaga aacccgcgga caaggcgagg
    19081 agccgaaacg cacgcgttat tttgaagttt cggcaacccc tgcctattcg agccggcaca
    19141 acaacggact tggcggcaat ttccaataca tcggccaatt gcccggctat ctgaaaatac
    19201 acggagaaat gcttgaaaac caatcactct tccggctgtc caaccgtgaa cgcaatcccg
    19261 acaaaccgtt tttagacatc cattttgacg aaaatggcaa aatcacgcgt attgtcgttt
    19321 acgaaaaaaa catctacttc aatccaaact tggggcgaag ataaaaaaat gccgtctgaa
    19381 acgttttcag acggcatttt tcaatgcaac aaatcaagcg cgtttgcgga attcgccggt
    19441 gcgggtatcg atttcgattt tgtcgccgtt ttcgatgtaa gacatcactt ggatttcggt
    19501 gccgccgacc aggcgcgcgg ttttcatcac tttgccggaa gtatcgcctt tgacggcagg
    19561 ctcggtatac tcgacttcgc gtacgatgat ggtgggcagt tctacggaga tggggttgcc
    19621 ttcgtagaag gtaacttcgc attggtcttc catgccgtca acgatgaatt tcaacgcgtc
    19681 gccgatgttg tcggcttcga tttcgtattg gttgaattct tcgtccataa agacgtacat
    19741 cggatcggca aagtagctgt acgtacagtt tttgcgggac aggatgacca cgtcgaattt
    19801 gtcgtcggct ttgtaaatgg tttcggaagc cgcgccggtc agcaggtttt tcagtttcat
    19861 gctgactttg gcggaagagc ggccgccttt gatgtattcg gttttttgaa cgaccatagg
    19921 atcgttgccg accataaata cattgccggc gcgcagttct tgtgctgttt tcattgtgat
    19981 tttccaatca aatcggttgt cgtaaaacgc actattctag cgtatttttt gatgctttga
    20041 aacaaaggcg gcgagttttt cggatgcgga aggctgcccg aaaagataac ggctccaatc
    20101 ctccgcgcct tgccgccagc cgttttgatg ttgttgcagg atttgccaac attcgaggcg
    20161 ttgcgtggcg gataaagccc ctccgccgtt gaggtcgtcc gaaagacggc ggtgtgccga
    20221 cacggtttcg ggcgtgtaga aaccgtgtgc cttatcccaa aaggcgtgga gtttgtcgag
    20281 atggacattc tcgtcttgcg ggtagatgtg ccaaaagaag ggtttgccgg caagctgggc
    20341 gcgcacgaaa ctgtcttcgc cgcgtatcac ggcgcagtcg gcgaggtgca gcagtttgtc
    20401 gaagtcctgt tgcggcacga aagggatttt gacgaggcgg acggatgccg tctgaaaaac
    20461 atcgccgtcg ttttgcaggg catcttgcgg aataacgccg ctttgtttga ggctgtcgat
    20521 gatttgcgtc cccgccagca acagtgtcat cgggctgcct gcctgtcgcc acatttccag
    20581 ccactttgcc caaacatcgc tccgatagcc gaaaagcagc cattcggggg cgtttttttc
    20641 gggaagcatc agccgctgac gcagggcttc gctatcgaaa cggacggctt cgcagtaatc
    20701 gcgttcgcgt atcaacccgc cgcttttttc gctgaaaccc ataaaccaaa aatatttttg
    20761 aacaccttcc tgcggcgaag gcatcaggtg cagcctttca ttgctttcct ccgcgctcaa
    20821 atattcccaa ttcagccaaa gcggcttgtg tcggcggata atgtgcagca cattttcggg
    20881 caggtcgcag gcaaaagttt cgatgacgac atcgggaaca ggcgcggtat caatatctgc
    20941 cgcatcggaa tgccaagtgc ggacatgaat atcctgatga acgcagggaa catcgggcaa
    21001 atcagggcaa agcgcacgca aggcggacac atcgtccgtc cacaaatgca cctgccaacc
    21061 gagttcgcgg tgcaaaacac gggcgagccg ccacgaaacg ccgatgtcgc cgaaattgtc
    21121 gatgaccttg caaaaaatcc aacagacaaa aggaggagta ttcatagaaa taacggaaac
    21181 gggctaaaac gcgccgaaat agtggaacaa atacacaacc agcaaaacaa tcggaataaa
    21241 cagcaacatg gtttgacgtt tcaaatcaaa ctcttgtttc aaatgatgct tgatggtgtc
    21301 gaaaaggacg aatttgaaca aaaacgtcgc ttcgcaggca aggatgatgc cgttgatcag
    21361 atgctggggt ttgtcttcgg gcggattggc gacgaaaacg gcaagcgtcg ccaaagggat
    21421 gaggcagacg acaagccagc ggacaagcag gtttcgggac attgcaaggc tttcaaaaaa
    21481 gcaaaacgcc attatcgccc caagcgcacg ccttgtctaa cggacgcgcc ggcgatgccg
    21541 tctgaagcct tgccgcagga aaacaccgca ccgccgaacc tttaaatacg cccgagacgg
    21601 gcgttccccg aaaaatcgga cgctgccgcc gatgcctgcg gacaaggctg catcgggcac
    21661 gggcatattg aaaacccggc gcgtcggttt gaaat
//
LOCUS       DAWKST010000015        20686 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.015, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000015 DAWKST010000000
VERSION     DAWKST010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20686
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.015"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(63..239)
                     /locus_tag="WJL87_000387"
     CDS             complement(63..239)
                     /locus_tag="WJL87_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798871.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMQTQAYLYEDLCLDLGGGKNP
                     GSYPICVIEKVPAR"
     gene            complement(242..1600)
                     /locus_tag="WJL87_000388"
     CDS             complement(242..1600)
                     /locus_tag="WJL87_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ7798872.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCAAMDGALKVDDTLAALQTLAKAWRENVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDDAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPNAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     IALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALALAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYACDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(1725..3371)
                     /locus_tag="WJL87_000389"
     CDS             complement(1725..3371)
                     /locus_tag="WJL87_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEZ7798873.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWRYCVSGGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMDKIASDKDLREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGAWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVQGEGNNLAFIDGLPDDPAPWYAGKGKSTKNTSKK"
     gene            complement(3394..4872)
                     /locus_tag="WJL87_000390"
     CDS             complement(3394..4872)
                     /locus_tag="WJL87_000390"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ7798874.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKFWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDEYGAELVGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGIAAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDKVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(4897..6642)
                     /locus_tag="WJL87_000391"
     CDS             complement(4897..6642)
                     /locus_tag="WJL87_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ7798875.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(6703..6966)
                     /gene="ftsL"
                     /locus_tag="WJL87_000392"
     CDS             complement(6703..6966)
                     /gene="ftsL"
                     /locus_tag="WJL87_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ7798876.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            complement(6963..7949)
                     /gene="rsmH"
                     /locus_tag="WJL87_000393"
     CDS             complement(6963..7949)
                     /gene="rsmH"
                     /locus_tag="WJL87_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ7798877.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQAALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PELPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPEQSQRKTSEWQ"
     gene            complement(7946..8401)
                     /gene="mraZ"
                     /locus_tag="WJL87_000394"
     CDS             complement(7946..8401)
                     /gene="mraZ"
                     /locus_tag="WJL87_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ7798878.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLLPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLGQTDLQL"
     gene            8680..9897
                     /locus_tag="WJL87_000395"
     CDS             8680..9897
                     /locus_tag="WJL87_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7798879.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQFSLKLQTLI
                     AEEIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTY
                     SARPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLTGYL
                     PQSHFLLNLGITELLAQVGKTDSPAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGVD
                     WAGFRFGDICHKL"
     gene            10029..10105
                     /locus_tag="WJL87_000396"
     tRNA            10029..10105
                     /locus_tag="WJL87_000396"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10063..10065,aa:Met,seq:cat)
     gene            10113..10188
                     /locus_tag="WJL87_000397"
     tRNA            10113..10188
                     /locus_tag="WJL87_000397"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10146..10148,aa:Ala,seq:ggc)
     gene            complement(10470..11291)
                     /locus_tag="WJL87_000398"
     CDS             complement(10470..11291)
                     /locus_tag="WJL87_000398"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ7798880.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIDFHSNHK
                     VFEITIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIVDVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFVAAFVSGLVAVKALLRFVSKKNYIPFAYYRIVFGIAIIILWLSGWISWE"
     gene            complement(11324..11968)
                     /locus_tag="WJL87_000399"
     CDS             complement(11324..11968)
                     /locus_tag="WJL87_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ7798881.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQKQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVKLSGL
                     KYQANPAVFKAVYEQKIRLENRSVAEKWALSQKGFDGKKLMRAYDSPAAAAAASKMQQ
                     LTEQYRIDSTPTVVVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(11971..12816)
                     /gene="ftsN"
                     /locus_tag="WJL87_000400"
     CDS             complement(11971..12816)
                     /gene="ftsN"
                     /locus_tag="WJL87_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ7798882.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPA
                     SSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAATEAEQSDAEKAADKADEVEEK
                     AGEPEREKSDGQAVRKKVLTEEREQTVREKAQKKDAETVKKQAVKPSKETEKKAPKEE
                     KKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTSDKAEATHYLQMGAYADRR
                     SAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHEVASLIR
                     SIESK"
     gene            complement(12922..14418)
                     /locus_tag="WJL87_000401"
     CDS             complement(12922..14418)
                     /locus_tag="WJL87_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ7798883.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECQVPQMPFEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDSQACIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(14432..14752)
                     /locus_tag="WJL87_000402"
     CDS             complement(14432..14752)
                     /locus_tag="WJL87_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ7798884.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            15235..16761
                     /gene="putP"
                     /locus_tag="WJL87_000403"
     CDS             15235..16761
                     /gene="putP"
                     /locus_tag="WJL87_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ7798885.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRAAR
                     "
     gene            complement(16851..17018)
                     /locus_tag="WJL87_000404"
     CDS             complement(16851..17018)
                     /locus_tag="WJL87_000404"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798886.1"
                     /translation="MKHFLDSPKFFGGQAASVRPEYGNIVINPQMKYKASNAGRVGAS
                     VSLKMKRVKHK"
     gene            17010..20615
                     /gene="putA"
                     /locus_tag="WJL87_000405"
     CDS             17010..20615
                     /gene="putA"
                     /locus_tag="WJL87_000405"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ7798887.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDKQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHCRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGTALTNDARIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSKKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDNVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRAGEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GNTVQAFAALTELAASGVQAVVEPDSPLASYTADLEGLLLVNDKPETAGISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         4837 a         5991 c         5053 g         4805 t
ORIGIN      
        1 cgggcctgcg cccgtttgag gttggcttgc cgcccggcaa ggtttcggac ggcttttgca
       61 gattaacgtg cagggacttt ctcaatcacg caaatcgggt aactccccgg atttttgccg
      121 ccacccaagt ctaaacataa atcttcataa agatacgcct gcgtctgcat tccggcataa
      181 aacgcgccga ccagcgcggc aatcaacaat aaggctttaa aaaaacgtcg acttttcatt
      241 ttcacttatc ctccaatgcc tcgaccactt cttccatctg cataaagcgc gaacctttca
      301 ccaacacggt ggcgcgttcg ggcaaatcgt ggcgcaacac ttgaatcaac gggtctttgg
      361 cggcgaacca caaaccgtcc gcgccaaatt tttccgccgc ttcgacgctg ttgtcgccga
      421 caaaataagc cgcttcgatg ccttggtcgc aggcatacgc gccgacttcg gcgtgcatag
      481 cggcggcttc gtcctcgccc agttcgccca tatcgcccat tacgaaaata cgcggcgcag
      541 gcatacgcgc caacacgtca atcgcggctt tcatgctgtc cgggttcgca ttataagtat
      601 cgtcaatcag ggttgcgccc ttgattccgg atttgacgtt cagacggcct ttgatattgc
      661 tgaaaccttt caaaccttcc gccacatcgt tcagactcaa acccgcagcc aaagccagcg
      721 cggcggcagc ggcggcgttg tggacattgt ggcggccggg aacaggcagc accacggcgg
      781 cgcgttcgtc gccgcacacc aaatcaaatt cgcacgacaa cggtttcagc acaatatttt
      841 ccgcgtgaac atcgccgcta tcgatgccga aagtgcgcgt attcaaatta agcgttgccg
      901 ttttgaagac agccatattg gcatcttctt gaggaatcag tgcaatgccg tctgaacata
      961 aaccttggta aatctcgctt ttcgctttgg caatatcgcc cactccgtcg aaaccgcagc
     1021 cgacatgggc gcgcatggcg ttgttgacca atgcggcatt tggtttggcg atttgcgtta
     1081 aaaccgccag ttcgccgaaa tggttcatgc ccatttcaat cacggcatag cggtgttttt
     1141 cgtttaactt caacaaagtc agcggcaatc cgatatggtt gttgaagttg cctgccgtcg
     1201 ccaacacggc atcatcgccg aaacggcggc gcaataccgc agccagcatt tccttcaccg
     1261 tcgtcttgcc gcccgaaccg gtaatgccga acacaaacgg attcacattt tcacgccacg
     1321 cctttgccag cgtttgcaat gcggcaagcg tgtcatcgac tttcaacgcg ccatccattg
     1381 cagcacaatc ttcgcgcgaa accacaaccg ccgccgcacc agcagccaat acgtcttcaa
     1441 caaaatcatg cgcgtcaaac cgcccgcccg ccaatgcgaa aaacacatcg cccgcgcgga
     1501 tgtcgcggct gtcggttacg atgcgcgaca cgggtttgct ttcagacggc atcggaagct
     1561 tgagggcttg gcagatgaaa tttaggtcca gtggtttcat atttactttc gttaatattc
     1621 gggcggcgga cacatcggta gcggctgatt tttttatcgc ctgttttgct gtggtaaaac
     1681 acagattatt ttcccattct cattcggcat tttttctgta cgtatcattt tttagacgta
     1741 tttttagtcg atttgccttt tcccgcatac cacggcgcgg ggtcgtcggg cagtccgtcg
     1801 ataaaggcaa ggttattgcc ttcgccctgc acatcgggaa cattccccca aaaatcatag
     1861 ccgtcatcgg gcaactcgtc ggtttcgata cccgtccaac tgccgaatcc gcgtaaaaaa
     1921 ttaaacgcgc tgcgcctcac tttaatcatt tcgcgccgcg tgtcatcgga cgaaatttta
     1981 accagcggca cgccgtagct ttgacgcttc cacgcaccat gtgtcaacgt tcgctcgccg
     2041 cctttaccga catgcatcaa accgtggtcg gaaaagtaaa ccaaagacca gctttcttta
     2101 ttttcattca atatcttaac tgtatcttct aaaaatttat cggtttgcgc gatggtggaa
     2161 acatagcagg atattttttc agtttgatac tgaaaccgcc gcgcatcctt atccaaacgt
     2221 gtgcaaaaat cactgtgcga acccatcagg tgcatcacaa tcagccgagg cttcgtgcct
     2281 gcatttccta tcaaaacccg tttgaacgcc ggcaacaaaa ggcggtcgct caaccccgcg
     2341 cttttgccat aatcacccct ttgggtaaac cacggataat cgctgcgtag ggcataggtg
     2401 gaaatttcgt tggcaaaatg ccccaacatt ccttgattag acagccacgc cgtccgaaaa
     2461 cccgcctgct tcgccaagct gacgatgtta ttgttcggtt ctcccggcag ccccaaagtc
     2521 tgcggcagcg aaagattcgt cgcgtgggcg gtcgattggt aaccgtttat caacagccct
     2581 ttggtctgac tcaaaaaagg cgtatcgggc aatgggaaac cgtaaacatt catataatcc
     2641 gaacgtgcgc tctcaccgat aaccacaaca taatttttat acttgggcgc aacatgacgg
     2701 atatgccatg ttgacgcttt ttttgctgct tccaaaatgt gggcgcgctt ggcggcatat
     2761 tgtgccggcg cggaagccaa atcgtaatac aggtcgaaaa tattcaacaa caggccggca
     2821 tcaggttctc gcaaatcttt atcgctggcg attttatcca tcaccgcgca ggacaaaatc
     2881 aaagtcaata atatagtcag ccatattttg ctgcggcgtt tatagttttt tacgtcagca
     2941 aatacccccc ccgatacaca atatctccaa actgtcaagc caaaaatgaa aaataatgcc
     3001 tgcacaaaat aaagcgaccc gggaagattg ccgacaaatt cacgcgcctc ggcaggattg
     3061 ctttccaata tcgaaccgac tatctgataa gacggcgcac catacaacca gccgaccggc
     3121 aaatataggg cggttgtgcc gacgtaaatc agtaaaacaa ctgaagaaac gcgcgggaaa
     3181 ccgcgtgcca ataataataa aaatacaacc gaagccaaag cacccacggc agaataacgg
     3241 tagccgtatt catattccaa gtgataacct gatgcgatgg ccgcgccaaa acaaaaggct
     3301 aaaagcgcgc aaatgcccaa tgtatttttt aatgtctgat ccatattttt tattcgggcg
     3361 gattgtttca tacgatgccg tccgaatatc ccattattta cgagttaaca aagcctgtcc
     3421 gacgatttca agatcggaaa aacggtgctt cacgccttgt acatcctgat agttttcatg
     3481 ccctttgccg gcaatcagga tgatgtcgtt tgcggcggct tgttcaaccg cataacggat
     3541 ggcggcggca cggtcggctt cgacgcattc gggcgcggga acggcaggca ggatgtcgtt
     3601 gatgatgtcg tgcggatttt ccaaacgcgg attgtcgctg gtgacgacga ctttatccgc
     3661 gccctgtacg gctgccgcgc ccatcagcgg gcgtttgccg cgatcgcggt tgccgccgca
     3721 accgaatacg caccacaaag ccgcaccctg cggtttgatt tcctgcaagg tggcgagtgc
     3781 tttttccaat gcgtcgggcg tgtgggcata atcgacaacg accaagggct tgccgctgtt
     3841 catgatgcag tccatgcgcc ctgaagcggg acggattttt gccagcacat ccaatacctt
     3901 atcaagcgga tagccgttgg cgcacagcaa ggcgatgcag gcggcgaggt tttgcgcgtt
     3961 gaaccgtccg agcaggcgcg tgcggcattt cccttcgccc cacggggttt ggaatacggc
     4021 tgctatgccg tctgaagagg cggtaaagtc ggtaatgcgg atgtcggcgt gttcgctgaa
     4081 accatagctg taaacggcca aatcgggaca gtcttttttc agacgaccta cgagttccgc
     4141 gccgtattcg tcatccacgt tgatgactgc gtgtttcaag ccgtgccagt aaaacaggcg
     4201 cgacttgatg gcaccgtagg cttccatcgt gccgtggtag tcgaggtggt cgcgggtgag
     4261 gttggtaaag attgcgctgc ggaatgacac gccgttgacg cgcgactggt caagcccgtg
     4321 gctggagact tccatcgcgg cgactgttgc gccttgttga cggaaacggt agagcagggt
     4381 ttggacatcg acgggggcgg gtgtggtatg cgtggtttct tccaatgcac cccaaaagcc
     4441 gttgccgacc gtgccgacaa tggcggtttt ttcgcccaac aaatcggcag cttgcgccag
     4501 ccattgcgtg atggaggttt tgccgttggt tccggttacg ccccagaatt tgagaccgtc
     4561 tgaaacgttg ccgtaaactt gcgccgccaa tatgccggca cggtgtttca aatctttgat
     4621 gccttgattg gggactttcc attcgggatt ccacgcaaat ttgccgtcgt cgtcccaaaa
     4681 aacaaaagcc gcgccgttgg caacggcggc ggggatataa ctgcgtccgt cggcatattc
     4741 gcccggacag gcaacgaaaa tatcgccttg tttgatttgg cggctgtctg aatgcaacaa
     4801 acgccctgcc gcgtttgcac acgacagagt cgggatgccg gtttcagcca aaggggttaa
     4861 cttgctgaac ataaaacaat ctcgttgata ctcggattaa gacggtgttt tgacggctgc
     4921 ggcggtcagt ggcttggtcg gggaaatgcc caagatgttc aggctgccgc ccataatttt
     4981 tttgaagggc ggccctgcca ctacgccgcc gtaataaccg tgggcagtcg gttcgtcaat
     5041 ggttaccgcc acaatcacac ggggattttt ggcgggggca aaaccgataa aggtagcgat
     5101 gtgtttgttg tcggcataac gcccgttgac gaacttgcgc gccgtgccgg ttttcgcgcc
     5161 gacatcgaaa ccgtccaccg cacccgccgt accggtgccg cccggctcgg ttacggaaac
     5221 catcagattg cgtacctcgc gcgcggtcga ttctttgaat atgcgtttgc cttgcggcgc
     5281 aaccgcctgt ttttcaaagc tgaccggcag taaaacgccg tcgtgcgtca gtgcggtata
     5341 ggcgcgcgcc aattgcagca ggctcaattg caggccgtaa ccgaaagaca tcgtcgcctg
     5401 ttcgataggc cgccacctgc gccaatttct caacaaacct gcagtttcgc ccggaaagcc
     5461 cgagtgcata cgcacaccga tgcccaactc atgatagaag tcatacattt cttcggcacc
     5521 gaaacgcgca gacagtttgc ttgtgccgac gttggacgat ttctgcatga tgccgcgcac
     5581 atccaaagag gggtaaacat gggtatcgcg cacgggagac ggtccgattt tataaggctg
     5641 cgtattcagc cgttcgttca aatcggtttt gcccgcatcc aatgccttcg caatcacaaa
     5701 cggtttgatt gccgaaccgg gttcgatcat atcggttacg gcacggttgc gccgctgttc
     5761 gctgtctgcc cggccgggcc tgttgggatc gtaggcgggc gtattggcca aggcgaggat
     5821 ttcccccgtg cgggcatcca aaaccaccac cgttccggct tttgcctgat ggtattcgac
     5881 cgccttgttc aactcttcat aggccaaggt ctgaatcctc tgatcgaggg aaaggatgat
     5941 gtctttgccg tttttcgggg ctttattgcg cggggagtcc aagctgtcca caatattgcc
     6001 ctgccggtcc cgcaaaacga cttccgcgcc gtcttcgcca tgcaggctgt cttcaagcga
     6061 aagttccaaa ccttcctgac ctttgccgtc aatatcggta aatccgatga cgtgtgcaaa
     6121 caggttgccc atcgggtaat ggcgttttaa ttctttttca aatacaaagt tttccaaacc
     6181 caaggctttg acctcttcgg caaccttggg atcgagctgc cgcttaatcc agataaacga
     6241 cttgcctttc tgttcgagct tgttcctcaa aacatcaacc ggcacatcga caagctcgga
     6301 caggcgttcc aattgtgcgg cagacggcat ttccttcatc tctttaggca cggcaaacag
     6361 ggactccgtc ggcgcactca acgccaaaac cgcaccgttc cggtccgaaa ccgtaccgcg
     6421 tgtagccggc aatgtttgag tccgcacaat ccggttgtcg ccctgttctt tcaaaaagtt
     6481 atacgttacc gtctgcagat acagtccgcg agcaatcaga ccggcaaaca agaccgctat
     6541 tgccatcagg acgaagctga tccgtccgtt actggtcatc ggctttttga cctgctcttc
     6601 tttgggcagc atccgaggct tatattcgct cttaatcaac atttttactt ctcgttatta
     6661 ttatcctgac gcaggaatcc gattccggca cacaggctgc ttctatcttt gatgctccac
     6721 cataaaggta ttgcccgaaa ccggcggatg gaggttttgt ttttctgccg ccgccctgat
     6781 cgcttcgtgg ttcgccaaac gcgcctgttg cagcctcatt tgcgcataat cctgctccaa
     6841 ggcgatttcc tgttttttcg ccttatccaa agctgtgaaa ttgagcctgt actggttttg
     6901 ctgcatcaca acggaaaaag cggaaacgca caccgcaagc agcagaagga aattcaattt
     6961 gttcattgcc attcagacgt ttttctctgt gattgttccg gtatcggacc ggcagtccgc
     7021 tccgccacac gcaaaaccgc acttctcgcc ctcggattgg cggcaatttc cgcctcaccc
     7081 ggctttaatg ccctgcccac gattttcagg ggcagctcgg gcaaatccgc ttccctgacc
     7141 gccgcccagc gcggcagggg cgcgtgttgc gaatattttt tgacaaactg cttcacaatg
     7201 cggtcttcca acgaatggaa agcaatgacc gccaaacgtc cgccctcttt cagacgacac
     7261 atgacctgcg gcaatactgc ccctacttct tcaagctcgc ggttaataaa gatgcggacc
     7321 gcctggaagg tgcgcgtcgc aggatcctgc ccccgctcgc gagtacggac gttttgtgcc
     7381 acgatctgcg ccagcttgcg ggttgtatcg attggacttt ccgcccgttg cgcaacaatg
     7441 gcgcgcgcaa tctggcggct aaaccgctct tcaccataat tcttgattac ctcgtgcaaa
     7501 tcctgttccg acgcaaccgc tatccactct gcggcagaca taccgcgcgt cgtatccata
     7561 cgcatatcca aaggggcatc gaaacggaag ctgaaaccgc ggctgccgtc atcgatttgc
     7621 ggagacgaaa tccccaaatc aaacagcgca ccgtccacct tgccgatacc caaaccgtcc
     7681 aatgccgcct gaaacgaagc aaaaccgcca tgcacgacac cgacccgttt gtccgaacgc
     7741 gccagctctt ctgccacagc aatcgcctga gggtctttgt cgaaaacaat caaccgcccc
     7801 gcatcgccca aacgcgacaa aatcagccgg gaatgccctc ccctgccgaa cgtaccgtcc
     7861 acatagacac cgtcttcgcg cacggcaagc gcatccaccg cctcattcag caagaccgtg
     7921 atatgccggt aactttctgc tccactcaca attgcaaatc cgtctgaccc aactggaagg
     7981 caagttcgtc aggatcgtca tccaaagcct gaaccatctc agcctcccac tgctcgcgac
     8041 cccaaagctc caaacggttg gcacgaccga ccaaaacgac ttcacggtcg aaatccaccc
     8101 ttttcctcag tccggcagga agcagcaccc ggccggcgct gtcccattcc aaaatttccg
     8161 cgttatgcag caaaagattt tgaaaccgcc gcaaaacagg gttatccgcc acttttaagt
     8221 ttaaaagttg cgccgcaacc ttttcccact ccgcaacagg gtacatcaac agcttgtgtt
     8281 tcgactcgag cgttaccact acggcaggcg tatagaggcg cgacagaatg tcacggaatt
     8341 tggcaggaac agccaaccgc cccttactgt cgatgcttaa ttcgtgtgcg ccgccgaaca
     8401 tgacatgtcc caagccgaaa tcaaaatcac aagggtaaaa gagacacttt gccccacaat
     8461 tccccaccaa tcgacactat aagaaatttt aaacactcgg tcaaatcagg gcatgaaaac
     8521 tcattaacat atctgaaatt tttattcctt ttaaaaacaa taagataaaa aatgacgaca
     8581 acggcacggc gggtgcggta cagaataatc gaaccaataa acaactatat atgattaatt
     8641 taataatata aacacaatat atagtattaa gataaagcca tgacagcacc cgtaccaacg
     8701 tgtaatatgt cgggaaatcc aataaattta cacaagctaa cacttatcat gcccctcccc
     8761 ctcccctctc ccgaagcacg gcaattctcg ctcaaactgc aaaccctcat tgccgaagaa
     8821 atcggcaaac acggcaactg gattccattt tcacgtttta tggaattggt tttatacgct
     8881 ccgcaatacg gctactacac cggcggcagc cataaaatcg gcaataccgg ggattttatt
     8941 accgcaccga ccctcacctc tctgtttgca cagacactgg cacgccaact tcaagaactt
     9001 ctatcccaaa cggcgggcaa tatctatgaa ttcggcgcgg gaaccggaca gcttgccgcc
     9061 gatttgttgg gcagcatttc ggacggcatc agccgttact atattattga aatatcgccg
     9121 gagctggcag cacgtcagaa aaacctgatt caagcacgcg caccggaagc atctcaaaaa
     9181 gttgtccact tgaccgcact tcccgaagcg tttgacggca tcatcatcgg caacgaagta
     9241 ctcgatgcca tgcctgtcga aatcgtccgt aaaaatgaag gcggctcatt cgagcatgtc
     9301 ggcgtttgcc tagataatga ccgttttacc tattcggcac gaccgctgca cgacttacag
     9361 ctatctgcct tggcttccct ctattttcct caaacagatt atccctacac cagcgaacta
     9421 catccgcaac aatatgcctt tatccgcacc cttgcctcaa ggcttgaacg cgggggtatg
     9481 atattcatcg actacggttt tgatgcagcg cagtattacc accctcaacg caatcaaggt
     9541 actctgatcg gacactaccg acatcacgtt atccacaatc cttttgactt catcgggttg
     9601 gccgacctga ccgcgcatgt caactttacc gacattgccc aagccggtac ggatgcagga
     9661 ctggatttga caggctatct gccccagtcc cattttctgc tgaatctggg cataaccgaa
     9721 ctgttggcac aagtgggaaa aacagactcg cccgcctaca tccgtgaagc tgctgccgtt
     9781 cagaaactga ttgaccagca tgaaatggga gaattgttta aagtcatcgc attcggtaaa
     9841 aatatcggcg tcgactgggc aggattccgc ttcggcgaca tctgccacaa actctaaccc
     9901 tcatgccgcc tgaatccgct tcagacgaca taaacttttt aacatttaaa aacagtcaac
     9961 taattcaaaa ttaaaaaata cggcttgtca aaaaaacaga aaaacatata atagcgtctt
    10021 cacgaaacgg cgaattagct cagtcggtta gagcagagga atcataatcc ttgtgtccgg
    10081 ggttcgagtc cctgattcgc caccaaattt tcgggggtat agctcagttg gtagagcgct
    10141 tgcatggcat gcaagaggtc agcggttcga tcccgcttac ctccaccaga taaaaaagca
    10201 cagaccgtaa aaaggtatgt gcttttttat tgcctgattg ccagcaaata aagaataaac
    10261 cactgccttc aaaacaggca atcgacttta aacctatcgc cccgcctgtc ctgattttat
    10321 agtgaattaa atttaaaccg gtacagcgtt ggctcgcctt gccgtactat ctgcggcttc
    10381 gtcgccttgt cctgattttt gttaattcac tatatcagcc cgccaaacaa acccgacccg
    10441 aataatgtct tcaggtcggg tttatggttt cattcccaac ttatccagcc tgacagccac
    10501 aatataatga tggcaatgcc aaaaacaatg cggtaatagg caaaaggaat ataatttttc
    10561 ttggaaacaa acctcagcaa cgcttttacc gccaccaaac cggaaacaaa agcggcaaca
    10621 aagccaatca gaatcaaacc gacatcatgc agggtgaaaa accggtaatg cttcaggaca
    10681 tcataagccg ttgctgcaac catcatcgga acggccaaga aaaatgaaaa ctccgttgcc
    10741 gttttccgct cgattcccca aagcatcccg cccataatcg tactgcccga acgggacgta
    10801 cccggaacca gtgcaaacac ttgggcaacg ccgatcatca acgcatcaat cggacgcaat
    10861 gcatcaacat cgacaatttt aggctctgcc cggctttggc gtttctccac ccacaaaata
    10921 aaaaaaccgc ccaaaaccag catgactgca acactcaagg ggttaaacag atactctttg
    10981 atttgtttgc cgaacaacag ccccatcacg gcggcaggta taaaagcaat ggcaagatta
    11041 aggacgaaac ggttggcttt ccggtctttt cccacgccat gcaacacatt gctgaaacgc
    11101 tgccggtatt caaacactac cgccaaaacc gcaccgagct ggatggtaat ttcaaaaacc
    11161 ttgtgattgc tgtgaaaatc aatcagattg ccgaacacaa tcaaatgtcc ggtgctggaa
    11221 atcggtaaaa attcggtaaa accttctacc aagcccatca tcagggcttt caggacaatc
    11281 agaaaatcca ttgcttgcgc ttctttcgga tacgggagtt cggctatttc tgtacagcag
    11341 gggtctgacg cttgcgttct tccctgactt tggcaaccaa ttctttaatc gtatgaacac
    11401 cgccgtcaaa gccattattg aagataacgc ggtattttcc gccgacgaca acggtcggcg
    11461 tactgtcgat gcggtactgt tccgtcaatt gctgcatttt tgatgcagca gccgctgccg
    11521 caggagagtc gtaggcgcgc atcagttttt tgccgtcaaa gcctttttga gacaaagccc
    11581 atttttcggc aaccgacctg ttttccaagc ggattttttg ttcgtaaact gctttaaaca
    11641 cggcagggtt tgcctgatat ttcaaacctg acagcttgac cgcagcagcc attcttgcta
    11701 gaccgagcat ttcaggctgc cagaccacgt gctccgtcct taaataggca tcagacggca
    11761 atgccttgcc caatttcaat aacaaaggat cgaaatgatg gcaatgtacg cagaaatagc
    11821 cgaaaaattc caaaacctca attttgcccg actgtttttg aggaatgggt ttatccaaca
    11881 caagatagtc ttccccttcc gtcagggcat atgcctgcgc ggacaacact gccgacagca
    11941 gcagcggcaa cagatgtttg agcttcataa ttatttactt tcgatagaac ggatcaggct
    12001 ggcgacttca tgttttttca actcgtcctg catttttttc accgcatcgg cagacatatt
    12061 gccgctttgc acccggtaaa gcgttttatg tcccgcctga tagccgacca ccttggaaga
    12121 tatgcccaag attgccagtt tggcacgctg cccttccgcg ctccggcggt cggcatacgc
    12181 gcccatttgc agataatgcg ttgcttccgc cttgtcggac gttttcattt tctgcacttc
    12241 tttggcggcg gcactgcgcg ctttttcgat gctgccgctg ttgaggattt gttccggggt
    12301 cggtttgggt gcaacttttt ccttttccgc ctttttctct tcttttggag cttttttctc
    12361 tgtttcttta gacggtttta ccgcttgttt tttaaccgtt tcggcatctt tcttctgcgc
    12421 tttttctctg acggtttgtt cacgctcttc cgtcagcact ttcttacgca ctgcctgtcc
    12481 gtccgacttt tcccgctccg gctcgcccgc cttttcttca acctcgtcgg ctttatcggc
    12541 agctttttcc gcatccgact gctctgcctc tgtcgcagca tccggttcgg acaaggcgtt
    12601 ttgatcggcc ggttcaggtt ggatgtcttc cttaggctgg tttttcggtt tcaggatttc
    12661 cgtttctgca ggctgcttcg acgaagccgg gattttgaac gcattttgac cgctctggtt
    12721 cagataaaac aaaataccgg caataatgac cgtcgccagt atcaaaccga agaaaaaacc
    12781 ggacagacct tttccggatt gggaaaattt gttcataaac ataccttaat gtgtttcaga
    12841 cggcattaac gccgtttgct tacgggcgga tattctaaca atatcgccat atttgggcaa
    12901 aacctgcttc cattcccatt cctataaagc acgacggaaa cctatggctt tcatgacgtg
    12961 gcttctgccg acttcttcgt cgccagccaa atccgccaat gtacgcgcca cgcgcatgat
    13021 gcggtggaag ctgcgggcgg aaagggagag tttttccagc aggccgccca atgcttcctg
    13081 cgcttctttt tggatgcagg cttgggaatc gagttcgctg acgctcaaag cggcgttgac
    13141 tttgccctgc cgtgcgtatt gtttgtcgcg ggcggcgata acgcgttcca aaacggacgc
    13201 gctgctttcg cccgcttcct gctgcatcag ttcggcggcg gacaggctgg ggacttcgat
    13261 ggtcaaatcg atgcggtcga gcagcggccc ggaaatcttg ctgcggtaac gcgcgacgct
    13321 ttcgggcgtg cagcggcagg gtttgacggg atgcccgaga taaccgcacg ggcaggggtt
    13381 catggcggca acaagttgga atttggcagg atagacggct tggcgcgccg cgcgggaaat
    13441 gtggatttcg ccgttttcca acggttcgcg caaaacttcc aaaactttgc ggtcgaactc
    13501 gggcagctcg tccaaaaaca atacgccgtg gtgcgccaat gaaatctcgc ccggacgcgg
    13561 gtcggaaccg ccgccgacca tagccgccgc gctggcgctg tgatgcggac tgcggaaagg
    13621 acggttgctg tcgagttgtt gctggtggtt gggcaggagc gaacgcaatg cccaaacttc
    13681 taccaattcg tcttcggtca gcggcggcag gatgccgggc agccgttggg agagcataga
    13741 cttgcccgtt cccggcggac ccatcatcaa gaggctgtgt ccgcctgcgg cagcgatttc
    13801 caaagcaagg cgcgcggtgt gctgaccttt cacatcgcac aaatcaggtt gtccgccatg
    13861 ttcaaacggc atctgaggaa cttggcattc ggtttgcgcc aaaggttcga tgccgttcaa
    13921 atgggcggcg acttcgccca aagagcgcgc gccgtaaacg gtaatgccgc gcatcacggc
    13981 ggcttgtcct gcattttctt caggcaaaac aaatgcacgt tttgcctgca taccctgcca
    14041 cgccatcgcc aacgcgccgc gcacggggcg caacagcccc gacagtgcca attcccccgc
    14101 aaactcgtat tccgccagtt tttcgggcgc aacctgcccc gatgcggcaa ggatgccgat
    14161 tgcaatcggc aaatcgaaac gccccgactc tttgggcagg tcggcggggg cgaggttgac
    14221 ggtgattttt ttggcgggga attcaaaacc gctttgaata atggcggcac ggacgcggtc
    14281 gcgactttct ttgacttccg tatcgggcag cccgacgatg ttgaaatgcg gcagaccgtt
    14341 ggcaaggtgg gcttccactt cgaccaacgg cgcattcata ccgctcaagg cgcggctgta
    14401 aaccaaggca agcgacatat ttcagacggc cttattcgcc ggcttcggtt tgctgcctga
    14461 tttcggcgac ggcttcttcg gcagcggctt cagccgcttc caatgctgcc cgttcgggat
    14521 tttgcgcggc ttcgagtttt gccaaacgcg cttccaaagc cgccagtttg gtacgggttt
    14581 tgattaaaac ctgctgctgg atgtcgaatt cttcgcgcgt aaccagatcc atacggttga
    14641 acgcgccgcc cagcatcgcc ttaatatttt tttccacatc tttggcaggg ctgttggcga
    14701 tggtttcgct gattttcgag ccgacttcct caaaaagctg cttgccgaac ataatctgta
    14761 tccttcctga acatatcaaa ttcaatcggc tattgtataa ggaaaaatgc cgtctgaaaa
    14821 cgggcggcgg ataatcggca aaacataccg cgcctccttt gcggttgcta aattacacct
    14881 caaaacacct gccgcctgaa aacggatttc atattccgcg caacgcctga ttacaagaca
    14941 ctacaaaaca atatgctgtt ttaaatgatt tttccgacgc gcatcgtttc aaactcggct
    15001 ttttaagcca ttaagtgctt tgcaaacaac agaaattggg ttatgctgaa acggattatt
    15061 tacaatttca tatagtttta ttacatatct tattgtgatt gaagataatt tatccgaatc
    15121 cccctttcgg gtatccggat tttccgttgt acttttatta gaaaaacatt tcaggcgcaa
    15181 gttgcttgca atttcaaagc ctgtaattct gaagttttag acgaggaata gcacatgaat
    15241 cccatgtaca ttacttttgc aatctatctg gttgcagtcc ttctcatcgg gcttgccgcc
    15301 tatttttcta cgcgcaattt cgacgattat attttgggcg ggcgcagcct aggcccgttt
    15361 gttaccgcga tgtcggcagg cgcgtccgat atgtccggct ggctgctgat gggtctgccg
    15421 ggcgcgattt acctgagcgg tttgaatgag gcttggattg ccatcggcct cttggtcggc
    15481 gcgtatttca actggctctt ggtggcgggc cgcctgcgcg tgcataccga atacgccaac
    15541 aatgcgctga cgctgccgga ttatttcttc caccgcttcg gcgcgggcgg acacttgatg
    15601 aaagtggttt ccgcactgat tatcctgttc ttcttcacga tttattgcgc ctcgggcatt
    15661 gtggcgggcg caaccctgtt ccaaagcctg tttgaaggta tgacttacaa tcaggcaatg
    15721 tggctgggcg cgggcgcgac catcgcctat accttcttgg gcggcttctt ggcggtcagc
    15781 tggacggata cgctgcaggc ttctttgatg attttcgccc tgattttaac gccggtgatg
    15841 gtctatctgg gcttgggcgg cgcggaacag atgtctgccg cgattcaaag cgtcgccgca
    15901 ggcacgggca aagaatacgg cagcctgttt gccggtacga ccgtcatcgg catcatttcc
    15961 accgccgcat ggggcttggg ctatttcgga cagccgcaca ttttggcgcg ctttatggcg
    16021 gccgaaagcg cgaaatcgct ggtatccgca cgccgcatcg gcatgacttg gatggcgttg
    16081 tgccttgcgg gcgcggtggc ggtcggttat ttcggcattg cgtatttcgg tgcaaatccc
    16141 gacaaagttt cttctatgag cggcaaccac gaacgcatct tcatcgcgct ttccaccctg
    16201 ctgttcaacc cttggatcgc cggcatcatt ttgagcgcga ttttggcggc ggtgatgtcc
    16261 accctgtcct gccagctttt ggtttgctcc agcgcgatta ccgaagactt ctacaaaggc
    16321 tttttgcgta aaaacgcgca acagtcggaa ctggtatggg tcggccgcct gatggtcttg
    16381 gcgattgccg tgatttccat cctgattgct tccgatccga acagcaaagt attgggactg
    16441 gtgtcttatg cgtgggcagg tttcggtgcg gcgttcggcc cgattgtgat tctgtccgta
    16501 ttgtggaaac gcatcactgc ctacggcgcg ttgtcgggta tggttgcagg tgccttaacc
    16561 gtagtcgtat gggcggaatg ggtaaaaaaa ccggctcaag cggcaggcga aagcggcttg
    16621 ttgacgatgt acgaaatcgt cccgggcttt attgtttgct taatcgtcgc cgtattggtt
    16681 tccctgttca acaaagaacc ttcgcgcgaa acccaagaac gctttgaaaa agccgacgca
    16741 gattaccgcg ccgcccgata aacccctttc agacggcacg gcatcccggt tcgtgccgtc
    16801 tgaacccgtt tccgggtcaa acacaaataa accttgcctg catacaggcc ttatttgtgt
    16861 tttacccgtt tcattttcaa agaaacggaa gcacctaccc tgcccgcatt tgaagccttg
    16921 tatttcatct gcggatttat tacgatatta ccgtattcag gccgcaccga tgccgcctgc
    16981 cccccgaaaa actttggaga atccaaaaaa tgtttcattt tgcatttccg gcacaaactg
    17041 ccctgcgcca agcgataacc gatgcctacc gccgtaatga aatcgaagcc gtacaggata
    17101 tgttgcaacg tgcacagatg agcgacgaag agcgcaacgc cgcctccgag cttgcccgcc
    17161 gtttggttac ccaagtccgc gccggccgca ccaaagccgg cggcgtggat gcgctgatgc
    17221 acgaattctc actctccagc gaagaaggca tcgcactgat gtgtctggcg gaagccctgc
    17281 tgcgtatccc cgacaacgcc acgcgcgacc gcctgattgc cgacaagatt tcagacggca
    17341 actggaaaag ccatttgaac aacagccctt ccctcttcgt caatgctgcc gcctggggcc
    17401 tgctgattac cggcaaactg accgccacaa acgacaaaca aatgagttcc gcactcagcc
    17461 gcctgatcag caaaggcggc gcaccgctca tccgccaagg cgtgaattac gccatgcgcc
    17521 tgttgggcaa acagttcgtc accggacaga ccattgaaga agccctgcaa aacggcaaag
    17581 aacgcgaaaa aatgggctac cgcttctcct tcgatatgtt gggcgaagcc gcctacaccc
    17641 aagccgatgc cgaccgctac taccgcgact atgtcgaagc catccacgcc atcggcaaag
    17701 atgcggcagg acaaggcgtt tacgaaggca acggcatttc cgtcaaactc tccgccatcc
    17761 atccgcgcta ctcgcgcgcc caacactgcc gcgtgatggg cgaactgttg ccgcgcctga
    17821 aagagctgtt ccttttgggc aaaaaatacg atatcggtat caacatcgat gccgaagaag
    17881 ccaaccgctt ggagctgtct ttggatttga tggaggcttt ggtttcagac cctgacttgg
    17941 ctggctacaa aggtatcggt ttcgttgtcc aagcctacca aaaacgttgt ccgttcgtta
    18001 tcgactacct gattgaccta gcccgccgca acaaccaaaa actgatgatc cgcctcgtca
    18061 aaggcgcgta ttgggacagc gaaatcaaat gggcgcaagt ggacggcttg aacggctatc
    18121 cgacctacac ccgcaaagtc cacaccgaca tctcctacct cgcctgcgcg cgcaaactgc
    18181 tttccgcgca agacgcggta ttcccgcaat ttgccacgca caacgcctac accttaggcg
    18241 caatctacca aatgggcaaa ggcaaagact ttgaacacca atgcctgcac ggcatgggcg
    18301 aaaccctgta cgaccaagtc gtcggcccgc aaaacttagg ccgccgcgtg cgcgtgtacg
    18361 ccccggtcgg cacacacgaa accctgctcg cctacttggt gcgccgcctg ttggaaaacg
    18421 gcgcgaactc gtctttcgtc aaccaaatcg tcgatgaaaa catcagcatc gacacgctca
    18481 tccgcagccc gttcgacacc atcgccgaac aaggcatcca cctgcacaac gccctgccgc
    18541 tgccgcgcga tttgtacggc aaatgccgtc tgaactcgca aggcgtggac ttgagcaacg
    18601 aaaacgtatt gcagcagctt caagaacaga tgaacaaagc cgccgcgcaa gacttccacg
    18661 ccgcatccat cgtcaacggc aaagcccgcg atgtcggcga agcgcaaccg attaaaaatc
    18721 ctgccgacca cgacgacatc gtcggcacag tcagttttgc cgatgccgcg cttgcccaag
    18781 aagcggttgg cgcagccgtt gccgcgttcc ccgaatggag tgcgacacct gccgccgagc
    18841 gcgccgcctg cctgcgccgt ttcgccgacc tgctcgaaca gcacaccccc gcactgatga
    18901 tgcttgccgt gcgcgaagca ggcaaaacgc tgaacaacgc cattgccgaa gtgcgcgaag
    18961 ccgtcgattt ctgccgctac tacgcaaacg aagccgaaca taccctgcct caagacgcaa
    19021 aagccgtcgg cgcgattgtc gccatcagcc cgtggaattt cccgctcgct atctttaccg
    19081 gcgaagtcgt ttccgcattg gcggcaggca acaccgtcat cgccaaacct gccgaacaaa
    19141 ccagcctgat tgccggttac gccgtttccc tgatgcacga agccggcatc ccgacttccg
    19201 ccctgcaact cgtcctcggt gcaggcgacg taggtacggc attgaccaac gatgcccgca
    19261 tcggcggcgt aattttcaca ggctcgaccg aagtggcgcg cctgatcaac aaagcccttg
    19321 ccaaacgcgg cgacaatccc gtcctgattg ccgaaaccgg cggacaaaac gccatgattg
    19381 tcgattccac cgcacttgcc gagcaagtct gcgccgacgt attgaactcc gccttcgaca
    19441 gcgcgggaca acgctgctcc gccctgcgta tcttgtgcgt ccaagaagac gttgccgacc
    19501 gtatgctcga catgatcaaa ggcgcgatgg acgaactcgt cgtcggcaaa ccgattcagc
    19561 tcactaccga tgtcggcccc gtcatcgatg ccgaagcaca gcaaaacctg ttgaaccaca
    19621 tcaacaaaat gaaaggcgtt gccaagtctt accacgaagt caaaaccgct gccgatgtcg
    19681 attccaaaaa atccacgttc gttcgcccca tcctgtttga attgaacaac ctcaacgaac
    19741 tgcaacgcga agtcttcggt cccgtcctgc acgtcgtccg ctaccgcgcc gacgaactcg
    19801 acaacgtcat cgaccaaatc aacagcaaag gctacgccct gacccacggc gtacacagcc
    19861 gcatcgaagg cacggtacgc cacatccgca gccgcatcga agccggcaac gtttacgtca
    19921 accgcaacat cgtcggcgca gtcgtcggcg tacagccctt cggcggacac ggtctgtccg
    19981 gcacaggccc caaagcaggc ggttcattct acctgcaaaa actgacccgc gccggcgaat
    20041 gggttgcccc gaccctgagc caaatcggac aggcggacga agccgcactc aaacgcctcg
    20101 aagcactggt tcacaaactg ccgttcaacg ccgaagagaa aaaagccgca gcggccgctt
    20161 tgggacacgc ccgcatccgc accctgcgcc gtgccgaaac cgtccttacc ggaccgaccg
    20221 gcgagcgcaa cagcatctca tggcacgcgc ccaaacgcgt ttggatacac ggcggcaaca
    20281 cggttcaagc ctttgccgca ctgaccgaac ttgccgcctc cggcgtacag gcagtggtcg
    20341 aacccgacag cccgctggct tcctacaccg ccgacctgga aggtctgctg ctggtcaacg
    20401 acaaacccga aaccgccggc atcagccacg ttgccgccct gtcgcctttg gacagcgcgc
    20461 gcaaacagga acttgccgcc cacgacggcg cactcatccg catcctccct tcggaaaacg
    20521 gactcgacat cctgcaagtg tttgaagaaa tctcttgcag cgtcaacacc acagccgccg
    20581 gcggcaacgc cagcctgatg gcggttgccg actgatttta ccgaaatccc cgagcgcggc
    20641 ccgtgaacca atgccgtctg aaaacctttc agacggcatt tttata
//
LOCUS       DAWKST010000016        20635 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.016, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000016 DAWKST010000000
VERSION     DAWKST010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20635
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.016"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..176
                     /locus_tag="WJL87_000406"
     CDS             <1..176
                     /locus_tag="WJL87_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798888.1"
                     /translation="LVLIFVNPLYNVGIGSGALLGHWVTQYSGISCIGIAGMLVSAAG
                     LWVCLNLNRHIRT"
     gene            305..1741
                     /locus_tag="WJL87_000407"
     CDS             305..1741
                     /locus_tag="WJL87_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ7798889.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSAQNLLLLAAGMGWLYHI
                     GPVFAAIIVLYSSCVYLLGELLRSDRENTRRFWLGCGIAASLTVLGFFKYFDFFRPLI
                     AQYAGKGGAIDILMPLGLSYYTFQSLAYLVYCFRAPHAARFSWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTRQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFNGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWFVTFHFVCLSFVVFNTASPD
                     DAGAVFNALFANANGWNAPQQANMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPISIILLLIIVLAPSGIPGFIYANF"
     gene            1752..2738
                     /locus_tag="WJL87_000408"
     CDS             1752..2738
                     /locus_tag="WJL87_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ7798890.1"
                     /translation="MKHFLSLFASILMSALIAAWFAQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYTLSDGIKAFLSGETPPTAQDGGSADMPPEAAASEAAPQTGEI
                     QEWKQDTDTAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYP
                     SFFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDR
                     ILEAAHTHRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLKGKIILIPTAHTLSGGKD
                     RYTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            2735..3928
                     /locus_tag="WJL87_000409"
     CDS             2735..3928
                     /locus_tag="WJL87_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ7798891.1"
                     /translation="MNSKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLQSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNTGDFPLGGILAHTGSGGSMTLTASDG
                     IASKQRVSLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLTIHTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTQWSKWRADRMNDLAQTGADLVILAYGTNEAFGDNID
                     IADTEQKWLDTVRQIHDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRIA
                     RQGQTMFWSWQNAMGGVCSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            4177..5730
                     /locus_tag="WJL87_000410"
     CDS             4177..5730
                     /locus_tag="WJL87_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEZ7798892.1"
                     /translation="MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPAGEMAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGIPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(5820..7847)
                     /locus_tag="WJL87_000411"
     CDS             complement(5820..7847)
                     /locus_tag="WJL87_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ7798893.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQAQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLENLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGNLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            8136..10142
                     /locus_tag="WJL87_000412"
     CDS             8136..10142
                     /locus_tag="WJL87_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ7798894.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TAPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGVIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRQPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRRTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
     gene            complement(10265..11356)
                     /gene="mltB"
                     /locus_tag="WJL87_000413"
     CDS             complement(10265..11356)
                     /gene="mltB"
                     /locus_tag="WJL87_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ7798895.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRVNEAQAPRADEMKKES
                     RPAFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKADIVKIMH
                     RPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAVIGIETN
                     YGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMG
                     MPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILVSATLAP
                     GADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEYYLGLNN
                     FYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(11505..12932)
                     /locus_tag="WJL87_000414"
     CDS             complement(11505..12932)
                     /locus_tag="WJL87_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ7798896.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKRHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AEHEDTAAFLLNDIRKDILDCRYCG"
     gene            13117..16815
                     /gene="mfd"
                     /locus_tag="WJL87_000415"
     CDS             13117..16815
                     /gene="mfd"
                     /locus_tag="WJL87_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ7798897.1"
                     /translation="MTYPIPKPREKSRWPNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKEHALPDLAVNRQSDEPLQALKDFQTAFEGRILLCAESLGRRETMLGFLQQNGL
                     KAKSVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSASATNPLPSPLPQEREQSAAVVSDSLKAAAVSTESSLPPGKSNLHGQIQQQPAPSP
                     VGEGWGEGKAVAAQSAIAVITESDLYQYVARSRVHNRRKKHAAVSDGLLRDLAEINIG
                     DPVVHEEHGIGRYMGLITMDLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHE
                     NVALHKLGSGAWNKAKRKAAEKARDTAAELLNLYAQRAAQSGHKFEINELDYQAFADG
                     FGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAV
                     LAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKAALEGLADGTVDIVIGTHK
                     LVQDNIRFKSLGLVIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGL
                     RDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRER
                     LETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIIN
                     RADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLA
                     MQDLEIRGAGEILGEGQSGEMIQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEI
                     KLHSPALLPESYCPDIHERLVLYKRLAVCETVQQINAIHEELVDRFGLPEQPVKTLIE
                     SHHLRLMAKELGIDAIDAAGEAVTVTFGKNNNVDPTEIILLIQNDKKYRLAGADKLRF
                     TAEMENIEVRINTVKNVLKTLQNRCLPK"
     gene            16964..17347
                     /locus_tag="WJL87_000416"
     CDS             16964..17347
                     /locus_tag="WJL87_000416"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ7798898.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTISGKRGSGVVCLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            17369..18211
                     /gene="kdsA"
                     /locus_tag="WJL87_000417"
     CDS             17369..18211
                     /gene="kdsA"
                     /locus_tag="WJL87_000417"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ7798899.1"
                     /translation="MDIKINGITVGNDAPFVLFGGINVLEDLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFREAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            18226..18666
                     /locus_tag="WJL87_000418"
     CDS             18226..18666
                     /locus_tag="WJL87_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ7798900.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIVAPVLFKMLPKMQAGEIA
                     SVLFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANRFLITPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSIVFMATALLSAVLSWRLSGKEAV"
     gene            18713..19999
                     /gene="eno"
                     /locus_tag="WJL87_000419"
     CDS             18713..19999
                     /gene="eno"
                     /locus_tag="WJL87_000419"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ7798901.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDASEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDAGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLESLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPGKAAFYQLGK"
     gene            20013..20291
                     /gene="ftsB"
                     /locus_tag="WJL87_000420"
     CDS             20013..20291
                     /gene="ftsB"
                     /locus_tag="WJL87_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ7798902.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(20332..20622)
                     /locus_tag="WJL87_000421"
     CDS             complement(20332..20622)
                     /locus_tag="WJL87_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ7798903.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYRKPPLAFLGRDEILPCCCYVEGDVRLDCGLSGEDEGLSDSLLK"
BASE COUNT         4912 a         6255 c         5140 g         4328 t
ORIGIN      
        1 gccttgtcct gatttttgtt aatccactat acaacgtcgg tatcggcagc ggcgcactgt
       61 tgggacattg ggtaacgcaa tactcgggca tttcctgcat cggcattgcc ggtatgctgg
      121 tatcggcggc aggtttgtgg gtctgcctga atttgaaccg ccatattcga acctgaccgc
      181 gcgatgccgt ctgaagcccc tcccgccctt cagacggcat tttgattgaa gaaatatccg
      241 cccctgctta aaataacagg ctttgccgtg ttttaacgcc tattttttgt tccaaggatg
      301 gtttatgccg ttgctgtctg tcgagttcgc actgttcttt ctcgctttcc tgccgattta
      361 ctggggcttg gcgaaatacc cgtccgccca aaacctgctg cttctggctg ccggtatggg
      421 ctggctctac catatcggcc ccgtatttgc ggcaatcatc gtcctttatt cctcctgcgt
      481 gtaccttttg ggcgaactgc tccgttccga tcgcgaaaat acgcgccgct tttggctggg
      541 gtgcggcatt gccgcctcgc tgaccgtctt gggctttttc aaatatttcg actttttccg
      601 gccgctgatt gcccaatatg ccggaaaagg cggcgcaatc gacatcctga tgccgctggg
      661 gctttcgtat tacaccttcc agtcgctcgc ctatcttgtt tactgcttcc gcgccccgca
      721 cgccgcgcgt ttcagctggc acgagctgct gctgcacctg agttttttcc ccaccgttac
      781 ctccggcccg attatccgcg ccgccgcatt caaaagcgca gacggcgagc aggcaggcgc
      841 attggcgcaa atccgtaccc gccaaccgcg ctcacccgtc cgccccgcac tcgccgtttc
      901 cctgattctg ctgggtattg ccaaaaaatg gtggctggcg gggatgctcg cagaaaactg
      961 ggtgtcgccc gtgtttgaaa accccgccca attcaacggc tggggcgtgt tggcgggcgt
     1021 gtacggctat accttccaac tcttcctgga cttttccgga tattccgatt tggttatcgg
     1081 catggcgatg ctgctgggct tccgactgcc caaaaacttc tccgcaccgc ttcgtgccgc
     1141 gaatatccgt gcattttggg acaaatggca catcagcctt tccacctgga tacgcgacta
     1201 catctacatc cccttgggcg gcagcaaaaa aggctttttg cggacacagc tcaacctgat
     1261 ggcggcaatg gtactctcag gcatctggca cggctacggc tggaacttcc tcatttgggg
     1321 cgctctgcac ggcacggcgc tggtgctgct caacacgggc gatcgctatt tcggacgcaa
     1381 cgcgctatgc cgtctgaaat accttgcgcc gctctcgtgg tttgttacct tccatttcgt
     1441 ctgccttagc tttgttgtct tcaataccgc aagccccgac gatgcaggcg cagttttcaa
     1501 tgccctcttt gccaatgcca acggctggaa tgcgccgcaa caggcaaaca tgctgctgct
     1561 tgcctcgttt gcatccgtga tgctgctcta cccttacctg caacgcgctt tcgacggcgc
     1621 ggtcaaaggt ttggaaaaaa tcccgatgtg gctgtggttt atcccgattt ccatcatcct
     1681 gctgctgatt atcgtccttg ccccctcggg gatacccggt tttatttatg ccaattttta
     1741 agggtttgga catgaaacac tttctttccc ttttcgcttc catattgatg tctgccctga
     1801 ttgccgcgtg gtttgcccaa aacccgatca atgcctactg gcagcagacc taccaccgca
     1861 acagcccgct cgaaccgctt gccgcctacg gatggtggcg gagcggtgcg gcgttgcaag
     1921 aaaacgccta caccctttca gacggcatca aagccttcct gtccggcgaa acgccgccga
     1981 cggctcaaga cggcggttcg gcagatatgc cgcctgaagc cgccgcatcc gaagccgccc
     2041 ctcaaaccgg cgaaatccaa gaatggaaac aggacaccga caccgccgcc gtccgcagcg
     2101 gcgacaaagt ctttttcgcc ggcgactcgc tgatgcaggg cgttgcaccc ttcgtgcaaa
     2161 aaagcctgaa acagcaatac ggcatcgaat ccgtcaacct cagcaaacaa agcacggggc
     2221 tgtcctaccc ctcattcttc gactggccga aaacgattga agaaaccctg aaaaaacatc
     2281 ccgaaatcag cgtgctcgcc gtcttcctcg gcccgaacga cccgtgggat ttccccgtcg
     2341 gcaaacgcta cctcaaattc gcttccgacg aatgggcgca agaatacctg aaacgcgtcg
     2401 accgcatcct tgaagccgca cacacgcacc gcgtccaagt cgtctggctc ggcatcccct
     2461 acatgaaaaa agccaagctc gacgggcaga tgcgctacct cgacaaactg ctttcggaac
     2521 atttgaaagg caaaatcatc ctgattccca ccgcgcacac cctgagcggc gggaaagacc
     2581 gctacacgga ctccgtcaac gtcaacggca aacccgtccg ctaccgcagc aaggacggca
     2641 tacactttac cgccgaagga caaaaactgc tggcggcaaa aataatggaa aaaatcgttt
     2701 ttgaaccaag tacgcaacca tcaagtacac agccatgaat tccaaacacc tcatcgcatt
     2761 ttccgccctg ttcgccgcca cgcaggcaga agccctgccc gtcgcctccg tcagcctcga
     2821 caccgttacc gtttccccgt ccgcccccta caccgataca aacgggctct tgaccgacta
     2881 cggcaatgcc gccgcctcgc cttggatgaa aaaactccaa tccgtcgcac aaggcagcgg
     2941 cgagaccttc cgtatcctgc aaatcggcga ctcgcatacc gccggcgact tctttaccga
     3001 cagcctgcgc aaacgcctgc aaaaaacttg gggcgacggc ggcataggct gggtttaccc
     3061 cgccaacgtc aaagggcagc gcatggcggc cgtccggcac aacggtaact ggcaaagcct
     3121 caccagcagg aacaacaccg gagacttccc gctcggcggc atcctcgccc acaccggcag
     3181 cggcggcagc atgaccctga ccgcatcgga cggcatagca agcaagcagc gcgtttccct
     3241 gtttgccaaa cccctgcttg ccgaacaaac cctgaccgtc aacggcaaca ccgtctccgc
     3301 caacggcggc ggctggcagg tactggatac gggcgcggca ctgcccctga ccatacacac
     3361 cgaaatgccg tgggacatcg gcttcatcaa catcgaaaat cccgccggcg gcattaccgt
     3421 ttccgcgatg ggcatcaacg gcgcacaatt aacccagtgg tcgaaatggc gtgccgaccg
     3481 tatgaacgac cttgcccaaa ccggcgccga tctagtcatc cttgcctacg gtaccaacga
     3541 agccttcggc gacaacatcg acattgccga cactgaacaa aaatggctgg ataccgtccg
     3601 ccaaatccac gacagcctgc ctgccgccgg catcctcatc atcggcgcgc ccgaatccct
     3661 gaaaaacacg ctcggcgtat gcggcacacg ccccgtccgc ctgaccgaag tccaacagat
     3721 gcagcggcgc atcgcccgtc aggggcagac gatgttctgg tcttggcaaa acgcgatggg
     3781 cggcgtttgc agcatgaaaa actggctcaa ccaaggatgg gccgccaaag acggtgtaca
     3841 cttctccgcc aaaggctacc ggcgcgcggc ggaaatgctc gccgacagcc tcgaagaact
     3901 cgtccgcgcc gccgcaatca ggcaataatc ggacaggagg cggacggtat ttccgcaaca
     3961 gggggatgcc gtctgaaacg cataccttca tattgcttca gacggcattc gcctcagaca
     4021 ttggaaagac gcaggcgaac ctccggcata cggcgcaaag gcggcgtttg atatgccgtc
     4081 tgaaggcaaa gatgataaac tgccgccttc cgttttcata cggcatattg ttttcaaatg
     4141 agggcgttcg ccgtccgcaa ccataaagga agtttcatga accggactta tgccaatttc
     4201 tacgaaatgc tcgccgccgc ctgccgcaaa aacggcaacg gcacggcagt gttcgacggc
     4261 aaggaaaaaa ccgcctaccg cgcgctcaag caggaggccg aagccgtcgc ggcgtatctg
     4321 caaaatatcg gcgtgaagtt cggcgacacg gtcgcgctgg cggtttccaa ttccacagaa
     4381 tttattaccg cctatttcgc cgtctccgcc atcggcgcgg tcgccgtacc gatgaacaca
     4441 tttttgaaaa acagcgaata cgcgtatatc ctgaacgact gcaaggcgcg cttcctgttc
     4501 gcctcggcgg gcctgtcaaa agagttggcg ggcttgaagg cgcaaacgcc cgtcgaaaaa
     4561 atcatttgga cggacaaaag ccgtccggcc ggcgaaatgg cagaaggcga tgcctttttt
     4621 gaagacgtgc gccgcttccc cgaaaaaccc gacttgggcc gccaaccccg gataaatgat
     4681 ttggcacaca tcatctacac ctccggcacg acggggcatc ccaaaggcgc gctaatcagt
     4741 tacgccaacc tgttcgccaa cctgaacggc atcgaacgca tctttaaaat ctccaagcgc
     4801 gaccgcttta tcgttttcct gccgatgttc cacagcttca cgctgacggc tatggtgctg
     4861 ctgccgattt atatggcgtg ttcgattatt ttggtcaaat ccgttttccc gttttccaac
     4921 gttttgaaac aggccctgct caaacgcgcg accgtgtttt tgggcatacc cgcgatttac
     4981 accgcaatga gcaaggcgaa aatcccttgg tatttcagat ggttcaaccg cattcgcctg
     5041 tttatcagcg gcggtgcgcc tttggcggaa caaaccatcc tcgatttcaa agccaagttc
     5101 ccccgcgcca aattgctgga aggctacgga ctgagcgaag cctctcccgt cgtcgccgtc
     5161 aatacgcccg agaggcaaaa agcccgcagc gtcggcatcc ccctgcccgg tttggaagcc
     5221 aaagccgtcg atgaagaatt ggtcgaagtg ccgcgcggcg aagtgggcga actgatcgtc
     5281 aggggcggtt cggtgatgcg gggctacctc aatatgcctg ccgccaccga tgaaaccatc
     5341 gtcaacggct ggttgaaaac gggcgatttc gttaccatag acgaagacgg ctttatcttt
     5401 atcgtcgacc gcaaaaaaga tttgattatt tccaaaggtc aaaacgtcta tccgcgcgaa
     5461 atcgaagaag aaatctacaa actcgatgcc gtcgaagccg ctgccgtcat cggcgtgaaa
     5521 gaccgttatg ccgacgagga aatcgtcgcc ttcgtccaat tgaaggaagg tatggatttg
     5581 ggcgagaacg aaatccgccg ccacctgcgt accgtgctgg caaatttcaa aatccccaaa
     5641 caaatccact ttaaagacgg gctgccgcgc aacgctacgg gcaaggtatt gaaacgggtg
     5701 ttgaaggagc agtttgacgg aaacaaatga acgccgtgcc gtccgaagcc ccgtccggca
     5761 aaaaaaatgc ggtgaatctg cttcaccgca ttttttggca aacgccggag cgaggatatt
     5821 tactctgcca attccacctg ctcgtgcgcc atcagttcct gaccgacatc atccaacaac
     5881 atcaaataac gttcgtagtt tttcaaaatg tcggcaatca gctcgtcctt gttcatatac
     5941 tgcacatcgt acccgacgcg cccgtcgaaa aaataagcgt agggtttgta agttgtctga
     6001 tgccggatat gcggcaggtt gccgtcgtta atcaactggt cggatacatc ctgcccgaca
     6061 gacttaatcc cgtacataaa atcgcgcatc gtctctttcc gaatgacgaa ctcgattgcg
     6121 ggctcgtcct gatgaaacat cttatcgacc cggacgctca agccgtattc ttccgaaagc
     6181 tcccgttgca actcgtgcat agcgggcgat gcggtatgtt tgaggaattt taaaatatcc
     6241 tgctcctgcg tctggctcat tatctgcacc agccgttctt tccacttgcc gcccgtccaa
     6301 aatacactgg tagggttaac ccgggtctca aaatatttct tatccgcact caatcctttc
     6361 cacaggctga aacacattat cagcatcagc agggcaaacg gcagggaaac aatcagggtc
     6421 atagactgca ggttgcctag cccgcccgag cgcatcagca aaacggcaac ggcagacatc
     6481 agcacgcccc acataaccgc ctgccaccgt ggtgcgctca agcccttgtc ccgagaggta
     6541 atattgttca ggacataaat cccggaatcg gcagaagtta caaaaaacag ggaaatgacc
     6601 agcaggctga cgatgctcgt caattcgggc agggggaggt aattaaagaa tttaaaaagc
     6661 agcgtttccg gagaggaggt catcttttcg agcattcccc ccgcaacccc gtcattcagc
     6721 caaatcgccg tattgccgaa gacggtaaac cacaaaacgc cgaacaggcc ggggatgagc
     6781 aaaaccccga agacaaactc gcggatggtg cgcccctttg aaatacgcgc gataaacaaa
     6841 cccacaaacg gcgcccaaga acaccaccac gcccaataaa gcaccgtcca agattcaaac
     6901 cacggcttgt gttcccgttc gtacgcataa gttttaaaac tgaggcgcac cagattttcg
     6961 aggtagttcc ctatgttgtc gccgaatgcc gacaacaggt aaacagtggg gcccgccgcc
     7021 aaaacaaaaa acagcagcaa aaacgcaagg ctcaggttca actcgctcaa caccttcacg
     7081 cccttcccca cgccggatat tgccgaaacg acggcgaggg acatgacggc ggcgataatc
     7141 aaaacctgca cgctgaagct gttttcggca atccagccca tttcctgcaa tccggcgccc
     7201 agttgcgaag ccccgaaccc caatgtggtg atgatgccga aaaaagtagc aagcaacgcc
     7261 ataatatcaa tggcatcgcc gaaccttccg gaaatttttt ctttcaacag ggggtaaaaa
     7321 caagaacgca gggcaagcgg cagcttgtag cggaaaccga aataagccaa agccaatgca
     7381 atcgtaccgt acaccgacca agcgtgaacg ccccaatgga acaccgtgtg cagcaatgcc
     7441 tgctgctgcc tgtgttccgg cgtgccggcc gtaatgtccg aaaaataatg catcaacggc
     7501 tctgccacgc cgaaaaacat cagacccacg cccatcccgg ccgcaaacag catcgccagc
     7561 cacgacagga agccgaattc cggcacatct tcatcccgtc cgagcctgat gtttcccaaa
     7621 ctgctgaccg agagtatcag caggaaaccc agaaaaatgg aaaacgttaa aacataaaac
     7681 cagctgaact cggtaaaaat gacttctttt gcccgatcga gccacatctg cgcctgatcc
     7741 ggcacggtta aaaccaatac caccaaaaca cacacaaaaa acaaagtcgt caaaataacc
     7801 atcggattaa atgacgttcg gcgttctata aattcagaca gggacaaacc ttctcactcc
     7861 tttgttaaaa acagacaaac ccggtcatcg ggcaaagcgg tcaaaacctg ccggtaaaat
     7921 accggcttcc ggatgacgaa atgcacaaac cggccgaaat tgttataatc gaaaaaatta
     7981 aaaatcaata cggattattc tatcagattg atttatcgat atttattata tcattaatat
     8041 agtttgattt caaaccggcg gaaaatgcgc cgcccaccgc aggcgcgccc tccccgctat
     8101 cggggcgttt aacgccggat tgccgatgcg gtacaatggc tgatatgaag aaaattaccc
     8161 ctcaaaacct gcgccccctg ctttcggaaa gcttgggaca taccgatttt gtcaacgtcc
     8221 tcaacgcact gattaaattt ttgcgccgtg gcggcaaaaa atgtgcgggg gaacgtttcg
     8281 acctgattat cgacacattc aaacaagaca gggaattgct gtcccgcttc agccggtgtt
     8341 tttacatctg gctcgcgcaa atacacattt atccggcact catcaaactc ggcatcttct
     8401 cgcgccacag ctttgcccgg gaaatgggca tacgcatcta cgaacgcttc agcccgtcat
     8461 ataaagattt tgccaacttg ggcgaagtct tcctttatct tttccattcc gaaaacgacg
     8521 acaaatggct gcaaacgctc aatatccgcc aatggctggt tttatacgaa ctcatccgga
     8581 gccacgccga accgtccaaa ttgcagacgg cgggcatccg ccttgccgat gcgcgtttgc
     8641 gcgccatcga aatgctgtct gtctggacgg catccgaagc catcgaaccc gacctcatcc
     8701 gcatcgcccc gcgcctgctg gaagccgatt cttccttcgt cgccctccaa cgcgaaaccg
     8761 ccaaactggt cgaacactac cgcaacggca ccgcgcctta cgacaccgcc cacctcgaag
     8821 tgatgttcga ccaatgtttc agccagattg actatttgcg ccgcaaaggg acgggcgccg
     8881 gctccggttc gtcggtcaaa gtcgcccacc tgctcgaacg gctccggcag accgtagacc
     8941 gtctgaagct gctcaccgac atccaaaccg gcgccggcaa cagcaaccgc ctgaccatcg
     9001 cgctgatgaa ctccctcatc tacgcggcgg tcgaacaata cagcacccgc cacctgcgcc
     9061 gcagcagcat ccgtatgctc gcccgcagca ttaccgaaaa caaaagccac cacggcgaac
     9121 actacatcac ccgcaaccgc aaagaatatt tcaaaatgtt ctactcggcg gcaggcggcg
     9181 gcgtcatcat cgccctgatg gcgctgctca aaatccgcat cggcacactc ggcctcagcc
     9241 ccttcctcac ttccttgtcg gccgggttca actacggcat cggctttatg atcatccata
     9301 tgctgcactg caccgtcgcc accaagcagc ccgcgatgac tgccgccagc tttgccgaac
     9361 aggtcgatct caacgaaggc ggcaaagcgg tggacaacaa actcgccaag ctcctcatcg
     9421 acgtatgccg ctcccaaagc gtcgccgtct tcggcaacgt ttccatcgcc atccttttgg
     9481 cgtgcgccat atcgttcggc tatgcccatc tgtaccggca gcccatactc gatgcccaca
     9541 ccgccgccta ccagttcaaa tccatagaca tcatcgctta cccgacgctg tggtatgccg
     9601 ccattgcagg tctgtggctg ttctgctccg gcatcatcgc aggttttttc gacaaccgcg
     9661 ccgactacct caacctgcgc caacgcctgc ctttcaaccc cttgctgcgt aaaatcatgc
     9721 gccccgggcc ccgccgcgtc ctcgccgcct acatccacaa acactacggc tcgttggtag
     9781 gcaacttcat cttcgggatg ctcttgggta tgaccggcta tttcggacac ctcctcgggc
     9841 tgccgctgga catccgccac gtcgcctttt cctccgccaa cctcggctat gccgccgtca
     9901 gcggcaacgt cggtttgggc acgttcgtac tcggcatttt cagcgtcctc gccatcggcc
     9961 tggtcaacct ctgcgtcagt ttcagcctcg ccctcttcgt cgccctgcgc tcgcgccgca
    10021 cgaaaatcgg cagcatccgc aatctgatta aaagtttttg gaatcagatt aaaagcaatc
    10081 cctgcatact tttcctcccg cccgccaaag aacagggaca tcctccttcg gacaagcctt
    10141 gaccggcaat gccgtctgaa gcgggattcg ccccgaatac cgccctgatg cgggaaatcc
    10201 ccataaaagg atgcaaaaat gccgtctgaa tcgaaacgtg gttcagacgg cattttaaaa
    10261 aacattacaa tcccgggccg ccaagcgaat tggcaatgtc cctgaccgcc gttacataca
    10321 tccgactgtg attgtactgc cataccgtat aaaaattgtt caagcccaaa taatattcaa
    10381 acacgccggg tgcggtttcc agtttgaaca aaaccgcctt ttcatcatcg gcaagctctt
    10441 cgccggggat gatgccgtac gccttcaaat ccgccaccgt ccgcgtcagg gcggtttttt
    10501 cgccaatgat tgcctgaaca tccgcacccg gcgccaatgt tgcagacacc agtattttcc
    10561 cgcccgtgcg ccaaccgtgc tgcttcatat aattggcaac cgatgccgcg acatcgccga
    10621 tgttgcccca tatgtcccga tgtccgtccc cgtcataatc caccgcccat ttccggtagc
    10681 tcgaaggcat aaattgcggc atccccattg cgcccgcata gctgccttta aaggcgaaaa
    10741 catcgccgcc ttcttctttt gccagcttta aaagctcgac caattctttt tggaaaaacc
    10801 cggcgcggcg ggggtaatca aagcctaagg tcgccaatgc gtccgccaca cggaaactgc
    10861 ccgtattttt gccgtaattc gtttcaatcc cgataaccgc cacgataagt tcggcaggca
    10921 cgccgtattt ttgcgccaca tcatcgataa gcgcgcggtt ttccgcataa aaccggcgcg
    10981 cgccgcgaaa tttcgccttg cccgaatttc ccgtgcggaa cacataccac ggacgcgatg
    11041 tggagggtcg gtgcataatc ttgacgatgt ccgccttgta agccgctttg tcaaaaaaat
    11101 cctgccattc cgcccgggaa aaatcccctt tcccgacttc atcgtccaca aaacggcgga
    11161 catttgcatt ggcggcaaac ccgctgtcgg ataccggtac ggctgccgcg tcaaacgcgg
    11221 ggcggctttc ttttttcatt tcatccgcgc ggggggcttg ggcttcattt acccggggcg
    11281 ggcgtgcctc catcgccgta caggcagaca aagccgccaa acaaattgcc agcggcagta
    11341 tttttctctt tttcataaat atgttccgaa caaatagggt aagtgggaaa gcggcgcaag
    11401 gggcgcggtg cgaaatgcgg catccgccgc aatcggcggc tttgtggaat accgcgcgtt
    11461 gccttttgca ccgcctgaaa tccgtatgcc gccgtatttg cccgctatcc gcaatatcgg
    11521 cagtccaata tatctttgcg gatgtcgttc agcaggaagg ctgcggtgtc ttcgtgttcc
    11581 gcctgtacga agacaaagag tcgggcgatg attttgccga tgaagacttt ttgcagggcg
    11641 caggcgtcgg tcagggtgtg ttcctgcagg aagctgcgga tgtcttcggg caaagtgtag
    11701 tcgcgggcga cggcggcaaa gcgggcatcg gtttgcaggc ggtcgagcag ggtttggttt
    11761 ttgtccaact tcatgcctgc ttctttttct tcggcggtgg cgcggacgaa ctggtcgtaa
    11821 atttcggcat aaagcatatc gttcgggcct tcaaaaatcg tgaagggacg gatgtcgata
    11881 gcgatattgc cggcggtgtg tccgcgttca aaacccttcg cgcccaaaag tttttgcaac
    11941 atttgcgcgg cggcgtaagt gtattccgtg gcgagggttt tgacgatgtt cgcctccatc
    12001 agctgatggg cgacgggtgc aacgggcgaa acggaatggc agacgtagcg gtaaagaatc
    12061 tcggaaacct gatggcggcg ccggatttcg cggcgttcgt aatcgacgaa tctgatgtcg
    12121 ttgcggacgt atcgttccag gttttcaagg atgtattcca taatgccgtg cgtcatgccg
    12181 atcagttgca ggcggctgcg gataaagatg ttttggaacg cgcgcaaacc tgcggcgtcg
    12241 ctctgggaga gtttcatcac ggcggttgca ggcatttcgg catcgatgcg gttgacggcg
    12301 taacggacgg cgcgcaagcc ttcggatgcg agggtttcgc agcggatgta tgttttgggg
    12361 acgagcagca ggtcgatgac tttggcgagt ttgccgtttt tgcgttcttt ggcggcaacg
    12421 aggaggaagt cgctttgcga gttgccctgc cagtattttg cggcgttgac gtaaatggtt
    12481 tgtccgtcgg tatattcgta gtaagactgc atttctcggg caatcgccgc gccggaggtt
    12541 tcgggttcgg taacgcccaa accgccgccc tcgcctttga aaaccatgtc caaaccctgt
    12601 gcgatttgcg cttcatcgcc gaactcttgc agtggctgca acaccagcgc gccttcgatg
    12661 ccggtacgca gcgtaacggg tacgccgtaa tgccccgcaa tccgcaatac ttcctgaatt
    12721 tcaaactggc tgcccttgcg gccgccgtgt cttttgtcga ggaagggcaa cagcaaaccc
    12781 gcctgcttca aggcaagcca tttgtcttcg ggcaggtatc gcatcaggtc gataccgtct
    12841 gaaaaaatgc ggcggaatgc ggattcgatg tgctttaaaa aagcagccgt gtccatagtt
    12901 gacggctgcg cgctcggttc ggtgtgtatc atcggcttcc tctgtcggtt cccattaatc
    12961 ggcggccggt caaaccgcct gccacagttt agagttgatt ttctaaactt taccacaaag
    13021 tgcgccgggc aacaatccgc cggcttttca gacggcatcg cgcaccctcc cgtgctaaaa
    13081 tgaccgtttg catcactgtc cgccgattgc cgcactatga cctaccccat ccccaaaccc
    13141 cgtgaaaaat cccgttggcc caatctttcg caaggctcgc tgcccttggc tttggcgcgt
    13201 tatctgccgc acaagcggct caaggtcgtg ctgacccaag atgcggaaca ggcgttgcgc
    13261 cttcagacgg catggcggtt tttccgtccg cacgacacgg cggtgttctt gccggactgg
    13321 gaaacgctgc cttacgagcg tttttctccg catcaggatt tggtgtcgga gcggctctcg
    13381 gcgttgtggc agattaagag cggcgcggcg gacgtgttgt ttgtgccggt tgccacggcg
    13441 atgcagaagc tgccgcccgt gccgtttttg gcggggcgca cgttttggct gaaaacagga
    13501 cagactttgg atataggccg tctgaaaacc gatttggtag atgcgggcta caaccatgtt
    13561 tcccatgttg ttgcggcggg cgagtttgcc gtgcgcggcg gcattgtcga tttgttcccc
    13621 atgggcagcg aaatgcccta ccgcatcgat ttgtttgacg atgaaatcga cagcatcaaa
    13681 acctttgata cggacacgca gcgcaccatc tcccccgttt ccgaaatccg cctgctgccg
    13741 gcgcacgaat tccccaccga cagcgaggcg caaaaaatct tccgcagccg cttccgcgag
    13801 gaagtcgatg gtaatccgaa cgatgcggct gtatacaaag cagtaagcaa cggccacttc
    13861 ggtgcgggcg tggaatatta cctgccgctg ttttttgaaa acgagctgga aacgctgttt
    13921 gactatatcg gcgaagacgc gctgtttgtc tctttgggcg atgttcacgc cgaggcaaac
    13981 cgcttttgga gcgacgtcaa atcgcgttac gccatggcgc agggcgacga aacctatccg
    14041 cctttgcttc cacagcattt gtatctctct gccgatgttt ttgcaggccg tctgaaaaac
    14101 tacggacagg tgctgcccga tgtttccggc aaggaacacg ccctccccga ccttgccgtc
    14161 aaccgacaat ccgacgagcc tttgcaggca ttgaaggatt ttcagacggc gtttgaagga
    14221 cggattttgc tgtgcgccga aagtttggga cggcgcgaaa ccatgctcgg cttcttgcag
    14281 caaaacggtt taaaagccaa aagcgtgtct gattggcaag gcttcttgtc ggcacatgag
    14341 ccgctgatga ttacagtggc accgttggct tacggattca aactgggcgg attgcaatcc
    14401 ccgaaccagc agcaaactac ttccgcctcc gaaggagaag gcgatgcggt taccgaccaa
    14461 accgaatttt ccgcatccgc aacaaaccct ctccctagcc ctctcccaca ggagagggaa
    14521 caaagtgcag ccgtcgtttc agacagtctg aaagcagccg ccgtttcaac cgaaagcagc
    14581 ctgccccccg gtaaaagtaa tctgcacggg caaatccaac agcaacctgc cccctccccc
    14641 gtgggggagg gttggggaga gggcaaagca gttgccgctc aaagtgccat cgccgtcatc
    14701 accgaatccg atctctacca atacgtcgcc cgttcgcgcg tccacaaccg ccgcaagaaa
    14761 cacgccgccg tttcagacgg tctgttgcgc gaccttgccg aaatcaacat cggcgacccc
    14821 gtcgttcacg aagaacacgg catcgggcgg tatatgggct tgatcacaat ggatttgggc
    14881 ggcgaaacca acgaaatgat gttgcttgaa tatgcaggcg aagcgcagct ttatgtgcct
    14941 gtttcgcaac tgcatttaat cagccgctac tccggtcagg cgcatgaaaa cgtcgccctg
    15001 cacaagctcg gcagcggcgc gtggaacaaa gcgaaacgca aagccgccga aaaagcgcgc
    15061 gataccgccg ccgagttgct caacctctac gcccaacgcg ctgcccaatc gggacacaag
    15121 tttgaaatca acgagttgga ctatcaggcg tttgccgacg gtttcggcta cgaggaaacc
    15181 gaagaccagg ccgccgccat cgccgccgtg attaaagacc tgacacaggc gaagccgatg
    15241 gaccgactcg tgtgcggcga tgtcggcttt ggcaaaaccg aagtcgccct gcgcgccgcg
    15301 tttgtggcgg tgatgggcgg caaacaggtc gccgtactcg ccccaaccac gcttctagtc
    15361 gagcagcacg cgcaaaactt tgccgaccgt ttcgccgatt tccccgtgaa agtcgccagc
    15421 ctttcgcgtt tcaacaacag caaagccacc aaagccgcac tggaaggctt ggcggacggc
    15481 acggtcgaca tcgttatcgg cacgcacaaa ttggtgcagg acaacatcag attcaaaagc
    15541 ttaggtttag tgattatcga tgaagaacac cgcttcggcg tgcgccagaa agaacagctc
    15601 aaacgcctgc gcgccaatgt cgatatcctt accatgaccg ccacgccgat tccgcgcacc
    15661 ctcagcatgg cactcgaagg cctgcgcgac ttctcgctga ttaccaccgc gcccagccgc
    15721 cgcctcgccg tgaaaacctt tgtcaaaccg ttcagcgaag gcagcgtgcg cgaagccgtg
    15781 ttgcgcgaac tcaaacgcgg aggacaggta tttttcctgc acaatgaagt agatacgatt
    15841 gaaaatatgc gcgagcggct ggaaaccctg ctgcccgaag cccgcatcgg cgtggcgcac
    15901 ggacaactgc gcgagcgcga gctggaacaa gtcatgcgcg actttttaca gcaacgattc
    15961 aacgtgttgc tctgttccac catcatcgaa accggtatcg acattcccaa cgccaacacc
    16021 atcatcatca accgcgccga caaattcgga ctggcgcaac tgcaccagct tcgcgggcgc
    16081 gtcggacgca gccaccacca agcctacgcc tacctgctca cgcccgaata catcaccaaa
    16141 gacgcagaaa aacgcctcga cgccatcgcg gcagcagacg aactcggcgc aggtttcacc
    16201 ctcgccatgc aggatttgga aatccgcggc gcaggcgaaa tcctcggcga agggcagtcc
    16261 ggcgaaatga tacaggtcgg cttcacgctc tacaccgaaa tgctcaaaca agccgtgcgc
    16321 gatctcaaaa aaggccgcca acccgacctc gacgcgccgc tgggcatcac caccgaaatc
    16381 aaactgcaca gccccgccct actgcccgaa agctactgcc ccgacatcca cgagcgtctc
    16441 gtcctctaca aacgcctcgc cgtctgcgaa accgtgcagc aaatcaacgc catacacgaa
    16501 gaactcgtcg accgcttcgg cctgcccgaa caacccgtca aaacccttat cgaaagccac
    16561 cacctgcggc ttatggcaaa agaattgggt atcgatgcca ttgatgcggc cggcgaagcg
    16621 gtaacggtaa ccttcggtaa aaacaataat gtcgatccaa ccgaaatcat cctgctgatt
    16681 cagaacgaca aaaaataccg ccttgccggc gccgataagc tgcggtttac cgcagagatg
    16741 gaaaatatcg aggtcagaat caacaccgta aaaaacgttt taaaaacctt gcaaaacaga
    16801 tgcctgccca aataaagccg acaccgcaat gccgtctgaa acaccgtttt ccttgtccga
    16861 aagccgccat tatgaatttg aaggaaactc cactataata cggcattcag atctccagac
    16921 ggcatcgcgc ccgtcagacc gcacacaaac caaaaggaaa tacatgttcc gtactatgct
    16981 tggcggaaaa atccaccgcg ccaccgttac cgaagccgat ttgaactacg tcggcagtat
    17041 taccgtcgat caagacctgt tagacgcagc aggcatctac cccaacgaaa aagtcgccat
    17101 cgtcaacaac aacaacggcg aacgttttga aacctatacc atttcaggca aacgcggcag
    17161 cggcgtggtc tgcctgaacg gggcggcggc caggctggta cagaaaggcg atatcgtcat
    17221 catcatgtct tacgtccaac tctccgaacc cgaaatcgcc gcacacgaac ccaaagtcgt
    17281 cttggtagac ggaaacaaca aaatccgcga catcatctcc tacgagccgc cgcacaccgt
    17341 gctgtaaccc tgaaaacgga cattgaccat ggatattaaa atcaacggca ttaccgtcgg
    17401 taacgatgct cctttcgtac tgttcggcgg catcaacgtt ttagaagatt tggattccac
    17461 cctccaaacc tgtgcgcatt acgtcgaagt tacccgaaaa ctcggtattc cctatatctt
    17521 taaagcctct ttcgacaagg caaaccgttc ctccatccat tcttatcgcg gcgtaggctt
    17581 ggaagaaggc ttaaagattt ttgaaaaagt caaagcagag ttcggcatcc ccgtcattac
    17641 cgacgtacac gaaccccatc agtgccaacc cgtcgccgaa gtgtgcgatg tcatccagct
    17701 tcccgccttt cttgcgcggc agaccgattt ggtggccgca atggcggaaa cgggcaatgt
    17761 catcaacatc aaaaaacccc agttcctcag cccttcgcaa atgaaaaaca tcgtggaaaa
    17821 attccgcgaa gccggcaacg ggaaactgat tttatgcgaa cgcggcagca gcttcggcta
    17881 cgacaacctc gttgtcgata tgctcggttt cggcgtgatg aaacaaacct gcggcaacct
    17941 gccggttatt ttcgacgtta cccattccct gcaaacccgc gatgccggtt ctgccgcatc
    18001 cggcggtcgt cgcgcacagg ctttggattt ggcacttgca ggcatggcaa cccgccttgc
    18061 cggcctgttc ctcgaatcgc accccgatcc gaaactggca aaatgcgacg gccccagcgc
    18121 gctgccgctg caccttttag aagatttttt aatccgcatc aaagcattgg acgatttaat
    18181 caaatcacaa ccgattttaa caatcgagta acacggtttc gccttatgat gcagactttc
    18241 cgaaaaatca gcctgtatgc cgcaaccttg tggctcggta tgcagattat ggcgggttat
    18301 atcgtcgcgc cggtgctgtt caaaatgctg cccaaaatgc aggcgggcga aattgccagc
    18361 gtattgttcg acatcctctc ttggagcggg cttgccgttt ggggcgcggt actggctgcc
    18421 gcctttgccg ccctaacccg gcggcaaacc gccctgctgc tttttttatt gtccgccctt
    18481 gccgccaacc gattcttgat tacacccgtt atcgaggcgc tgaaatacgg gcatgagaat
    18541 tggctgttgt cgtttgtagg cggatccttc ggaatgtggc acggcatttc cagtattgtt
    18601 tttatggcaa ccgccctact ttcagcagtt ttaagttggc ggctttccgg caaagaggcc
    18661 gtctgaagcc ctcccatttt ttacctccct tcacttcact tggagaacat tcatgagcgc
    18721 aatcgttgat attttcgccc gcgaaatttt ggactcacgc ggcaacccca cagtcgagtg
    18781 tgatgtattg ctcgaatccg gcgtaatggg acgtgcagcc gtaccgagcg gtgcgtccac
    18841 cggtcaaaaa gaggctttgg aactgcgcga cggcgacaaa tcccgttatt cgggcaaggg
    18901 cgtattgaag gcggtcgaac acgtcaacaa ccaaatcgcc caagccctca ttggtatcga
    18961 tgccagcgaa caatcttata tcgaccaaat catgatcgaa ttggacggta ctgaaaacaa
    19021 aggtaacttg ggtgccaacg cgactttggc cgtttctatg gccgtagcac gcgcagccgc
    19081 tgaagatgca gggctgccgc tttaccgcta cttgggcggc gcaggtccga tgtccctgcc
    19141 cgtaccgatg atgaacgtca tcaacggcgg cgaacacgcc aacaacagcc tgaacatcca
    19201 agagtttatg attatgcccg tcggcgcaaa atctttccgc gaagcgttgc gctgcggtgc
    19261 ggaaattttc cacgccttga aaaaactgtg cgacagcaaa ggcttcccga ccacagtcgg
    19321 cgacgaaggc ggtttcgccc ccaacctgaa cagccacaaa gaagccctgc aactgatggt
    19381 cgaggcgacc gaagccgccg gctacaaagc gggcgaagac gtattattcg cattggactg
    19441 cgcctccagc gagttctaca aagacggcaa ataccacttg gaagccgaag gccgctccta
    19501 caccaacgcg gaatttgccg aatatctgga aagtctggtc aacgagttcc ccatcatctc
    19561 catcgaagac ggcatggatg aaaacgactg ggaaggctgg aaactgctga ccgaaaaact
    19621 gggcggcaaa gtccaactcg ttggcgacga cctcttcgtt accaacccga aaatccttgc
    19681 cgaaggcatt gaaaaaggcg tggcaaacgc actattggtc aaagtcaacc aaatcggtac
    19741 tttgagtgaa accctgaaag ccgtcgactt agccaaacgc aaccgctacg ccagcgtaat
    19801 gagccaccgc tccggcgaaa ccgaagacag caccattgcc gacttggcag tcgccaccaa
    19861 ctgtatgcag atcaaaaccg gttctttgag ccgttccgac cgcatggcga aatacaacca
    19921 actgctgcgt atcgaggaag aattggcgga agccgccgac taccccggca aagccgcatt
    19981 ctaccaactg ggcaaataaa aaaggttaag gtatgaagtg ggtaactgtc gttttatcct
    20041 tcgcacttgt ctgttgccaa tacagcctct ggttcggcaa aggcagcatc ggacgcaaca
    20101 gcagtctgag agaacagatt gccgttcaag aagaaaaaaa ccagacactc gccctacgca
    20161 atcattccct tgccgccgaa gtctatgatt tggaaaacgg tcaagaagcc atttcggaaa
    20221 tcgcccgggt agaactgggt tatatccaag acggtgaaac cttttaccga ctcatcaggc
    20281 ataaccggta ataccgtcaa aaagccgtcc gaaccaatgt tcggacggct tttatttcaa
    20341 caaactgtca gacagcccct catcctcccc cgacaaaccg caatccagcc tgacatcccc
    20401 ctcgacgtaa cagcagcacg gcagtatctc gtcccgcccc aaaaaagcca aaggcggctt
    20461 ccgataagta acgcttccct ccaaaatctt cacccggcac gatccgcaat atccgcttcg
    20521 gcactgatat tccaccatat gccccgtccg ttccaagcct tccaacaggg tctcgccctc
    20581 caagagctca aaaaaaccct tattcgtacc aatgcgcgcc atttccgacc aatca
//
LOCUS       DAWKST010000017        19476 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.017, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000017 DAWKST010000000
VERSION     DAWKST010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19476
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.017"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(284..2059)
                     /gene="ptsP"
                     /locus_tag="WJL87_000422"
     CDS             complement(284..2059)
                     /gene="ptsP"
                     /locus_tag="WJL87_000422"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ7798904.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYNAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRILLGMGLRRFSMNPNNILPVKNIILHSNIAQLENEIAKIVRCEDE
                     EKAEKLIKQMNSVSVEEEADFKGRK"
     gene            complement(2059..2328)
                     /locus_tag="WJL87_000423"
     CDS             complement(2059..2328)
                     /locus_tag="WJL87_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ7798905.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            complement(2397..2834)
                     /locus_tag="WJL87_000424"
     CDS             complement(2397..2834)
                     /locus_tag="WJL87_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ7798906.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            complement(2915..3478)
                     /locus_tag="WJL87_000425"
     CDS             complement(2915..3478)
                     /locus_tag="WJL87_000425"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ7798907.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            complement(3547..4371)
                     /locus_tag="WJL87_000426"
     CDS             complement(3547..4371)
                     /locus_tag="WJL87_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ7798908.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLVSRQGYVFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     ASSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRYGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTQKGTPKFATFVRVRTDR"
     gene            4463..5095
                     /locus_tag="WJL87_000427"
     CDS             4463..5095
                     /locus_tag="WJL87_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ7798909.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFTLPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            complement(5302..7086)
                     /gene="rmuC"
                     /locus_tag="WJL87_000428"
     CDS             complement(5302..7086)
                     /gene="rmuC"
                     /locus_tag="WJL87_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ7798910.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFADLNAQLAEKAA
                     RCDFVEQAHAEIASELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLADVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQHDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQSSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERSNETTALSESLEYAAEDEAV"
     gene            complement(7425..8213)
                     /locus_tag="WJL87_000429"
     CDS             complement(7425..8213)
                     /locus_tag="WJL87_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ7798911.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            complement(8228..9577)
                     /locus_tag="WJL87_000430"
     CDS             complement(8228..9577)
                     /locus_tag="WJL87_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ7798912.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSSGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            complement(9596..10177)
                     /gene="petA"
                     /locus_tag="WJL87_000431"
     CDS             complement(9596..10177)
                     /gene="petA"
                     /locus_tag="WJL87_000431"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ7798913.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            complement(10300..11049)
                     /locus_tag="WJL87_000432"
     CDS             complement(10300..11049)
                     /locus_tag="WJL87_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ7798914.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            11180..12109
                     /locus_tag="WJL87_000433"
     CDS             11180..12109
                     /locus_tag="WJL87_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ7798915.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGAPLFERKSTPLRFTPVGERLLRLAHDLLPQVTDAERDLARIIEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPIWPQIELDIVSGFQADPIGLLLQHRADLAIVSEAEKQNGIS
                     FQPLFAYEMVGICAPDHPLAAKTVWEAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            complement(12365..12757)
                     /gene="rpsI"
                     /locus_tag="WJL87_000434"
     CDS             complement(12365..12757)
                     /gene="rpsI"
                     /locus_tag="WJL87_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ7798916.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(12767..13198)
                     /gene="rplM"
                     /locus_tag="WJL87_000435"
     CDS             complement(12767..13198)
                     /gene="rplM"
                     /locus_tag="WJL87_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ7798917.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(13376..13555)
                     /gene="ssrS"
                     /locus_tag="WJL87_000436"
     ncRNA           complement(13376..13555)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WJL87_000436"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(13561..13866)
                     /locus_tag="WJL87_000437"
     CDS             complement(13561..13866)
                     /locus_tag="WJL87_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ7798918.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(13877..14206)
                     /locus_tag="WJL87_000438"
     CDS             complement(13877..14206)
                     /locus_tag="WJL87_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798919.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QWQETQQ"
     gene            complement(14261..15250)
                     /locus_tag="WJL87_000439"
     CDS             complement(14261..15250)
                     /locus_tag="WJL87_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ7798920.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(15373..18075)
                     /gene="ppc"
                     /locus_tag="WJL87_000440"
     CDS             complement(15373..18075)
                     /gene="ppc"
                     /locus_tag="WJL87_000440"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ7798921.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRNMEHDFQMVYPDVCVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAALLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPRSRINIVPLFETIEALENACPVMETMFRLDWYDALLESCGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAAVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            18118..19011
                     /locus_tag="WJL87_000441"
     CDS             18118..19011
                     /locus_tag="WJL87_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ7798922.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDAFLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGIGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAEALADRLKRINHNVDVRTVNEKLDGCRL
                     TGLVQTADIVLDCCDNYATRQAVNRVCVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGCTQAAEALKILLDVGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPICGTER"
BASE COUNT         4187 a         5276 c         5159 g         4854 t
ORIGIN      
        1 aaatttatcc gaagcgacaa caatcttttc atcgtcattc ccgcaaaagc gggaatctag
       61 aacgtaaaat ctaaagaaac cgttttaccc gacaagtttc cgtgccgaca gacctagatt
      121 cccgcctgcg cgggaatgac ggattttagg tttctgattt tggttttctg tttttgaggg
      181 aatggccgat tttgggtttc tgtttcggtt ttctattttg caggaatggc aaaatttcag
      241 attgcgggca ttgttaagta tttctatttt ttacctgccg tatttatttc cgccccttga
      301 agtcggcttc ttcctcgaca gacacgctgt tcatctgttt gatcagcttt tccgcctttt
      361 cctcgtcctc gcagcggacg attttggcaa tctcgttttc gagctgtgcg atattgctgt
      421 gcaggatgat gtttttgacg ggcaggatgt tgttcggatt catggaaaaa cggcgcagcc
      481 ccataccaag caggatgcgg gtaaacgccg tatcgcccgc catctcgccg catacggaca
      541 cgtctttgtc catgcggttg gcggtgcgga tgacgtgttg cagcattttc agcacggcgg
      601 gatgtccggg ctggtaaaga tggctgacgc tgtcgtcgcc gcgatcgacg gacaagatgt
      661 attgaatcag gtcgttggtg ccgacggaga tgaaatcgac cagtttcaaa atactgccga
      721 cggtcagcgc ggcagacgga atttcaatca tacagccgat gccgacttta ccgaaggcat
      781 cgccgcgttc ggcaagctgg cgttgcgcgg tgtcgaggtg gatgaggcac tggcgcactt
      841 cggatacgga ggtaatcatc ggccacatca tgcggacggg accgtgtacc gccgcacgga
      901 ggatggcgcg catctgggtg cggaacatga ccggttcggc aaggcacagg cggatgccgg
      961 tcatgcccag cgcggggttg aggctgccgt tgggcgtgct gtttttcccg aaccagcgcg
     1021 ggtttttgtc cactcccaaa tcgactgtcc gtatcgttac gcttttgccc ttcatttttt
     1081 tgacaatcgc gttgtacact tcgtactgct cgtcttcaga cggcatcgta tcgcggttga
     1141 ggtaaagaaa ctcgctgcgg aacagcccga tgccgtctgc gccgaggttg tgcagcggtt
     1201 tcacgtcttc ggcggattct atattgccca caagctcgat gcagaccccg tcggcggtgg
     1261 cggcggcggt ttttttgagc ttgttcaaat cgcgtttgtg gctgcggtat tcgcgggcac
     1321 ggcggcggta ttcgttcaac accgactcat ccggcgcgat aatcaacacg ccgttgatac
     1381 cgtccacaat gaccgtttcg ccttcggtaa tcagtttgcg cgcgttgtgc agcccgacga
     1441 cggacgggat gtccaagctc ctgcccaaaa tcgccgtatg cccggtgggg ccgccggcat
     1501 cggtaacgaa ggcggcaatg cgctgctctt taaacaaaac cgtgtcggcg ggcgaaaggt
     1561 cgtttgcaat cagaacggtt tcgtcaaaca ggttgtcggc aacttccaac tcgttgccct
     1621 gcccgatcag gttgttgtgg atgcggcgga cgacttgcag catatcctgc ttgcgttcgc
     1681 gcaaataggc atcgtccata ttgtcgaatt gggcggcgag tttgtcgctc tgctgcttca
     1741 atgcccactc ggcgttgatt ttttgttccc ttaaaatatc gacgggttcg cgcgacaagg
     1801 taacatcggt caagagcatc aggtgcagcg agatgaacgc gcccaactcg gtcggggcgt
     1861 tttcgggaat cgcgctgcgg agctgttcca actctttgcg cgtggctttg acggcggcat
     1921 cgaaacgttc ggcttcggca tcggtgtccg cctccgcaac atcatactgc ggcacttcct
     1981 ccgtaccgcg cgcaatcagg tgggcgcaac cgacggcaat gcctttgccc gccgccacgc
     2041 cgtgcagcac gatactcatt attcgccctc gccgaagtag ccgttgatta agtcggtcag
     2101 ggcgcgcatc gcttccgcct cgtccgcgcc gtcggtttcc agctcgatga ccgtaccctt
     2161 ggcggcggcg agcatcatca gccccataat gcttttgccg ttgacgcggc tgtcgttttt
     2221 cgtaacccag acttcgcttt tgaattggga cgcggtttgg gtgaacttgt tggacgcgcg
     2281 ggcgtggagt ccgagtttgt tgatgatttc gatggattgt ttgagcattt cggttcccgt
     2341 gttatgtata tcggcagcag acgccgttta aaatgttttc ctgccctgcc gcttcttcag
     2401 acggcatcgc cgctgcgccg gcacaccaaa tcttcgggcg cggacgtgat ggcgaaaatg
     2461 ccttttacag ccgcctctct gacacattcg gtaaaggcgg caaggtcttc cgccgccggc
     2521 gaatattgga cggccttaac catcatcggc gcgttcagcc cggtcaaaat cgccgatttg
     2581 ttttcgcgca cgaggcggcg ggcggcattg cagggggtcg cgccgaagat gtcggtcata
     2641 atcagcacgc cgtcgttgtc gggaaattcc tgaagcgcgg caatggcgtt gttgatgatg
     2701 tcgtcttggt cttccgtcgg ttgcacgccg agtatgcgga cgttttcagg cagcccgccc
     2761 gggaaaaaat gatgtgccag cttgcggtag gcttcgccta tggtttcgtg tgtgatgatt
     2821 aaaagcccta tcatattatg cgtcctgttc ctcgttgtcc tgccggcgta tgggcgcaat
     2881 gccgtctgaa aggcattcag acggcatcgc gtccttattt tccgtccaat gcgtaaatct
     2941 cgcccagatt gcgccagcag cccgccgcat ccatgccgta accgaaaaca taacggttcg
     3001 gcacatccag tccgacataa tcggctcgga taggcttggg tttgtcgatc agcttgttgg
     3061 cgaacaccgc cgcacggcag cttgccgcac ccatttccaa aagtttggct tgaatggcgg
     3121 acatcgtatg cccttcgtcc aaaatatcgt ccagcacgac gacgtgcctg ccccggattt
     3181 gttccgcatc gggcatacgc ttccagttga acgcgccgcc ctccagcttg tcgccgtaac
     3241 gggaaacgtg aacataatca aaatctaagg gaaaacgcaa cagcggcagc aactgccccg
     3301 taaacaccac cgcgccgccc atcacgggca gcagcagcgg atatttgccg cccaaatcgc
     3361 gcgtaatctc gtccgccact ttttgcagtg cggcacggca ttggccttgg tcgaacaaaa
     3421 gatcggcgtt ttcaagcatc gcctgtgttt caaggcgttt ggtttctaaa tcggtcatat
     3481 gtcggaatcg gtcggtaaaa ggaaaattat aaaccaaagt atcggatgcc gtctgaactg
     3541 tcctgctcag cggtcggtac gcacgcgcac aaacgtggcg aatttcggcg tgcctttctg
     3601 cgtaaagcca cggtagcggt aggtaatcag tgtgccgatt ttgggcgggt tgtctcggtc
     3661 tttatctttg aagccgctgc caatgcggaa ttcgccgtac cggtttttgc agccgaccgc
     3721 gcccaaccgt ccggcgtttc gccctttgcc ctcatagtgc cgcgttaccg tgcattcgtc
     3781 gtcgtattgg cttttcagct tcaataattg gctgctcctg ccgccgctgt aacgggattc
     3841 gggctgacgc agcatcacgc cttcgccgcc ctgcgcttcg atttgtttta aaaagtccat
     3901 cgcgtgctgc cggtcgcgca ctttgatttg tgggatgatg gtaatcggcg cgttcggatg
     3961 cgttttcagc cactgcgttg cgactgccaa acgttggtag agattgccct gcgccttggg
     4021 tacatcgaaa acgtgcaggc ggataccgcg ccagtctgaa gaagcagaac gcacggtagc
     4081 ggaaatctgc tcgaactgac cgcgtccgct atacaattcg ccgtccaaag gataaggcgg
     4141 aaactgcgcg gtaaagcctt tgggcggtgt aaatacatat ccttgccggc tgactaaatg
     4201 tttaccgtcc caatacgcgc gcacgccgtc gagtttctcg ctcatcgccc agcctgcaat
     4261 gtcctgccct ttgtattcct gcgccagcat caaatccgcc gcgcctgctg atgcagggat
     4321 gaaaaccgcc gtaaaaatcg gtatgatgcc gccgattgtc ttcttaatca tctgattccc
     4381 ccaatatcaa aacgggcggc aaaccgccat aaaacaaacg gcaaacccga tgccgtctga
     4441 aaaaccgttt aggaacacgc cgatgaccct acgttacgaa atcttccccg ttaccccctt
     4501 ccgccaaaac tgcaccctga tttgggacga cgaaagcggc gaagccgtcc tgaccgatgt
     4561 cggcggcgac gtgccgttcc tgctgcaagc gttggcaaac cgcaaactta cgctcacggc
     4621 aatctggctg acgcacggcc atctcgatca cgcgggcggc gtggtcgaaa tgttgaaaac
     4681 gcataaagtc cctgtcctcg ggccgcatcc ggacgatgaa ttcctgctcc aatcgctgcc
     4741 gcaaaccacc gcgcaatacg gatttcccgt ctcgcccgcc tttgcgccga accgttggct
     4801 cgaagaaggc gaaacgctca cggtcggacg ctatgccttt caagtgctgc atattccggg
     4861 ccatacgccg ggacatatcg tcttttattg tgccgaggcg gaattgctga ttgcgggcga
     4921 cgtgctgttt tacgaaacca taggcagaac cgattttccg cgcggcaacc acgccgactt
     4981 aatcaataat atccgcaaca aattattcac ccttcccgaa accgtgcaag ttgtcgccgg
     5041 acacgggcgt atgacttcca tcggacacga aaagcggcac aatccgtttt tctaaccgcc
     5101 ttccctacgg tcttcagacg gcatcatctg cactgatgcc gtctgaaaca caaaaggctc
     5161 agacaaccgc cgccttgccg gtgtacctcg ccagacaagg ctttggtaag tacttcaaac
     5221 aaacccaaaa tcagaaccag tacagttacg ccgcgctttc ggcattcccg ccccggctga
     5281 aacaatattt ttccgcacaa gtcagactgc ttcatcttct gccgcgtatt ccaaagattc
     5341 cgacaacgcc gttgtttcat tggaacgctc gaccaaatcc cgttgaagtt gtttgcctgc
     5401 cttcacgccc aacagacgca gtttctcggc gcgtccgacc agattcccgc gcccttcggc
     5461 aagttgcttg aatgccgtct gaaaactgct ttgcgcctga tcgatgcctt tgccgacgct
     5521 ttcgagcgtc tgtacgaagc cgacaaactt gtcgtacagc ttgccgcctt cgtccgcaat
     5581 cgccagtgcg ttctgatttt gctgttcgtt gcgccaaata ttcgccaccg tcctcaaagt
     5641 cgccagcagc gtactggggc cgaccagcat aatccgtttg tcgaaacact cttggaacaa
     5701 tcccgcgtca tgctgcaacg ccagcaggta ggccggttcg acagggataa acataaagac
     5761 gaaatccaat gtgttcacac cttccaaatc ggtgtaatcc ttcagcgaca agcctttcat
     5821 gtgtgcacgg atgctggcaa cgtgtgccgc cagttcgcgt gccgccgtat ccgcatccgc
     5881 cgcctgcgtg tagcgcacat aagctgtcag cgagaccttg gagtcaatca caatctgctt
     5941 gttgtcgggc aggttgacca aaacgtcggg ctggaggcga cgcgtgccgc cgtcttcctc
     6001 ttttcggacg gatgccgcct gaaccacata ttcgcgccct ttctggaggc cggaattttc
     6061 caaaaccgtt tccagaatca tctcgcccca attaccctga accttgttct gcgtgccggt
     6121 cagcgcgttg gtcagggcct ttgcctcgct gtgcagctgc gcattcaatc cttgaagccg
     6181 tttcaattcg ttttccaacg tcagccgctc gcgcgattct ttatcatagg tttgcttgac
     6241 caactcgccg aaaccgtgga tgcgttcgtt tagcgggttc aaaacctgat ggagctgctc
     6301 gcggttctgc tcggtaaaac ggcggctttt ttcttccaaa atcgtgttgg caagattttg
     6361 aaactgatcg ctcaagctct tgcgcgcctc gcccagtaag gacagcttct cttcagaagc
     6421 aaggcgttcc tgttcgattt gcgttgccaa acgttcgttt tcaaccgcca aaccctgtgc
     6481 cttttcctgc aactcggtat gcgactgcct caaccgctcc gcttccgcct ctttttcctg
     6541 caaatgggca atctgttttt cggctgcggc aaaacggttg ccgacatcgg caaggtcgtt
     6601 ttgcacgtcg cggacagttt ggcggctttc ttccaaatcg gtttcgattc tttggcggat
     6661 ttggcgttcc aaggcatatt ggtcggcaaa cgccttccgt tcctgtccga gctgcgttgc
     6721 caaacgttcg ttttcaaccg ccaaaccctg tgccttttcc tgcaactcga tatacgactg
     6781 cttcagccga accgattccg cctctttttc ctgcaaatgg gcaatctgct tttcggctgc
     6841 ggaaaaacgg ttgcccaaag cataattttc gtcctgcaaa tgtcggtatt tcccgtccaa
     6901 aacggccaat tccgatgcaa tttctgcgtg tgcctgttcg acaaaatcac atcttgccgc
     6961 cttttccgcc agttgcgcgt tcaaatcggc aaactcgccc tgaaacctgc ccttcatcag
     7021 caaccatgta aacaacacgc ccgacaccaa cgccgccaaa ggcagcaaaa cagtcatcag
     7081 ttccatcaat catcctaata ttcaaacatt ttcacaccgg acaaaaccgc cgcttattcc
     7141 gattctacct gtttgttcga catagctcca aaaaatatag cggattggct ttaaacctgt
     7201 tcgacatcgc cttaccatgc tgcttgcggt ttcagacctt ttcctaattc aatatcaatc
     7261 tgccacaaac cctgattaag ttcccgatgt ctgacatttt tagaatgatg ccgtctgaaa
     7321 tgttgcagct atgttcagac ggcatacgga tttaggcttt tcaaacggca ggcaaaatga
     7381 aaaaagggca aaccctaaag gatttgccct tttgttccaa acgcttagtg tacgtctttc
     7441 caatattctt ttttcaggaa atacgccaaa ggcagcataa ccgcaaatag gaaaatcatc
     7501 acgacatagc ctatacgttt gcgttgcagt tgtgcaggtt cgcccatgta cacaaggtaa
     7561 ttgaccaaat cgcgtacata tgcgtcgtac tctttttgga tcactttgcc gttaggcaga
     7621 cggcggctgt gcaaaccggt agattcccaa tacagcttag gcttcatctc gccgtgttcg
     7681 tcttttacca taaccggctg acctttggca tccaactcaa cggcttgaac gccttgctgt
     7741 tcccacaacg ggtggggcat accgacttta tcgaatacag tattgttcca gccgctcgga
     7801 cgggtcggat ctttatagaa gccgcgcata taagcgtaaa ggtagtctgc acctttggaa
     7861 cgcgcaatca atgtcaaatc gggcggagca gcaccaaacc attttgccgc atctttcggg
     7921 ttcatcgccg aatgcatgac atcgccgaca ttatcggtgg taaacatcag gtttttcttg
     7981 atttcttcgt cagtcaaacc gatgtctttc aaacggttga aacgcatacc gcttgccgag
     8041 tggcaggaca ggcaatagtt ggtaaagatt tgtgcgccgc gctgcaggct gacttggtcg
     8101 cgcaaatcga tatcgacttt ctcatagtgt gcatgaccgc ccgaagcaaa tgccgcaccc
     8161 atgggtactg ccaacagcaa ggcagcaaac caatttttca tagcttgttt cattttgact
     8221 gccctcatca gatgttggtt gcaaacaggt aagcacccac ggcggtaatg ccgacgtaaa
     8281 caaagaacat gattttttgt ttggcggtgc tcatggttac gcgttcggga accggtttgt
     8341 ttttatccag cttggtatag aacggcatac ccaggaagaa tgcgaaatag acgaaggaca
     8401 ggatgcgcgc gaccaaagta cgcgtatcgg ttgccaccat cgcgcccaaa atacccaaac
     8461 cgataaagga aatgatgaac aagaccaatg cggttttgaa aatcggaccg cggtaacgga
     8521 cggatttcac ctcgccccta tccagccaag gcagcagggc aatcaacacg accgccgcgc
     8581 ccataccgat tacaccccaa acctgcgttc ccaagaacga gggaatcgca cgcaaaatgg
     8641 cgtagaacgg tgtgaaatac cataccggcg caatgtgcgg gggtgttttc agcgcatttg
     8701 ccgcatcgaa gttcggcgct tcgaggaagt agccgccgcc ttccggtgcg aagaacaata
     8761 cggcacagaa gacaatcagg aatacaacca cacccaagat gtctttaacg gtatagtaag
     8821 gatggaatgg gataccgtcg cgcgggatac cgttttcgtc tttgtttttc ttgatttcaa
     8881 cgccgtcagg gttgttagaa cccacttcgt gcagggcgat gatgtgcgcc acaaccaagc
     8941 cgagcaatac cagaggtacc gcgataacgt gcagggcaaa gaaacggttc agggtaacgt
     9001 cggaaacgtt gaagtcgccg cgaatccaag tggacaaatc cggaccgata acagggatgg
     9061 cggagaacag gttaataatt acctgcgccc cccaaaagga catctgaccc caaggcagca
     9121 ggtagcccat aaaggcttcc gccatcaatg ccaagaaaat cagagaaccg aaaatccaca
     9181 ccaactcacg gggttttttg tacgaaccgt aaatcaaacc acggaacatg tgcagataaa
     9241 caacaatgaa gaagaaagat gcgccggtgg aatgcatgta gcggataatc caaccgctgg
     9301 acacgtcgcg catgatgtac tcgactgcag taaaggcagc aggcagatgg taggcgttca
     9361 ggttgccgtc gggtttgtag ttcatggtca ggaaaatgcc gctgacgatt tgaatcacga
     9421 ggacaagcag ggccaaagag ccgaagtagt accaaaaatt aaagtttttc ggtgcgtagt
     9481 attgagccaa atgctcattc cacattttag acagggggaa acgagcgtcc atccagccta
     9541 acaatgcttt tgctttgcta ttggtttggt ttgccataat tatcgttcct tattcttagt
     9601 cttcgcccac caagatagtt gtgtcgctca agtatttata tggcgggaca accaggttgg
     9661 tcggggcagg aacaccttta tatacgcggc cggccaagtc gaatttcgaa ccgtggcagg
     9721 ggcagaagaa gccgcctttc cagtctgcac ccaaatcggc gggggcaatg tcgggacgga
     9781 aggtgggcga gcagcccaaa tgggtgcaga taccgatggc gacaaggatg ttcggcttaa
     9841 tcgaacgggt ctcgttttta gcatactccg gctgctgttc cgcatcggaa ttgggatcgg
     9901 taagttcgcc gttcaggcct ttcaggtctt taagctgctg atctgtacgg ttgagcaccc
     9961 aaatcggttt gccttgccac tcggcggtca gcagctgacc cgcttcgatt ttactgacat
    10021 ccacctcgac ggcagcaccg gcggccttgg ctttttccga agggaaaaaa ctggccacaa
    10081 acggcgttgc cacacccaat gctgccactc cgcccgcgcc gcaggtcgcg agtgtcagga
    10141 aacggcggcg gccgttgttg atttcttgat tatccattat tcagtcgtcc taatattttg
    10201 ggaataccga gccattaaac gttgcaattt tacccagttt gcagtgatac tcaaagcatt
    10261 atttaaaata aggtaaagtt ttatgatatt tctcaagact caagccggat tgttttcgtc
    10321 aaaatggcac acttccaacc cgaaaacctc tgccgccgat tctgccagcg cgcgtacgcc
    10381 gtaacgttcc gtcgcgtgat gccctgccga aatgaaagcc gtacccgttt cattggcaag
    10441 gtggtattgg gcttcggaaa tttcccccgt caaatacaga tcgacacctt cgtctattgc
    10501 cgtctgaaaa aacccctgcg ccccgccgct gcaccatgca acccgtcgga tttcgcgttc
    10561 gggtttgccg atgacgacag gcttacgttg caaaactgtt tcaatatgcg ccgccaatgc
    10621 gccgagtgtc ttggcttgtt tcaggctgcc cgagttgagc aggttttgtt cgccgaaccg
    10681 tttttctgtc gcaaaaccca atctgtcggc gagttgggca ttgttgccca gtgtgggatg
    10741 tgcatccagg ggcagatggt agcctgccat attgatgtcg tgccgtaaca gtgcggcaat
    10801 ccgttctttt ttccaaccag taacggtcgg caactcgttt ttccagaaca taccgtgatg
    10861 taccaaaagc aaatctgcct tctgctccac agcaaaatca atcgctgccc tgcttgccgt
    10921 taccgacgta acgattttcc cgatatattc cctcccttca acctgcaaac cgttaggggc
    10981 gtaatcttta aacaacgctg tctgcaatgt ttcattacac caagtcagaa aatccctgcg
    11041 caataccatc ttttttccta atcgctttaa acaagcgggc attctaatcg caaaatgtct
    11101 ggaattcaca tttttccgat ttgcacccgc atatgaatta ttttaatatg cgccggttca
    11161 atatgccgtc tgaaacctca tggattccat catcgaactg cgccacctca agaccctgct
    11221 ggcacttgaa gaaaccggca gcgtctccct tgccgccaaa cgggttttcc ttacccaatc
    11281 tgcactttcc caccaaatcc gtatgcttga aaaccactac ggcgcaccgt tgttcgaacg
    11341 aaaatccacg cccttgcgct ttaccccggt tggagaaagg ctgttacgcc tcgctcacga
    11401 cctgttgcct caagttaccg atgcagaacg tgatttggca cgcattatag aaggagaggc
    11461 aggagaattg cggattgccg tcgaatgcca tacctgtttc gactggctca tgcccgcaat
    11521 gggcgaattc cgcccgatat ggccccaaat cgaattggat atcgtatcgg gattccaagc
    11581 ggaccctatc ggactgctgc tgcaacaccg tgccgacctt gccattgttt ccgaagcgga
    11641 aaaacaaaac ggtatttctt tccaaccgct gtttgcctac gaaatggtcg gcatttgcgc
    11701 accagaccat ccgcttgccg ccaaaactgt ctgggaagcc gaagacttta tcggggaaac
    11761 cctgattact tatcccgttc ccgacgagat gctggatttg cccaaaaaaa tcctgattcc
    11821 gaaaaacatc aacccgccgc gccgacacag cgagctgacc atcgccatta tccaactggt
    11881 tgccagcaga cgtggcattg ccgcccttcc ctattggaca gtcatgccct accttgaaaa
    11941 aggctatgtc gttcaccggc agattactgc cgacggattg caaagcaaac tgtatgccgc
    12001 catccgtacc gaagatacgg acaagagcta tctgaacaat ttttgccaaa tcatacgcga
    12061 acgcggtttt gcagatttgc ccggactgag cgaactggaa ccggtctgac cccttattca
    12121 accatacccg gcagtttttc tattttttca tgtatagtgg attaacaaaa accagtacag
    12181 cgttgcctcg ccttgccata ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt
    12241 tttgttaatc cactatactg tttttgattt ttgcccaatc tgtaatcttt agattgccaa
    12301 tgggaaaccg tctactacaa ataaaaaaac cctgcgataa gcagggtttt ttgaatttcc
    12361 aacattaacg tttggagaat tgttttgcac ggcgtgcttt gcgcagaccc ggttttttac
    12421 gttcgacttc gcgggcatcg cgggtaacaa aaccggcttg agacaaggcg ggtttcaacg
    12481 cggcatcgaa gtcgatcagg gcacgggtaa tgccgtggcg gattgcgccg gactggccgg
    12541 tttcgccgcc gccaacaaca ttgactttga tgtcgaaaga ttcggcgttt tcagtcagaa
    12601 ccaagggttg gcgaacaacc attcggctgg tttcgcgtgc gaagaattcg tcaacgggac
    12661 ggccgtttac gatgatttga cctgtacctt taatcaggaa tacacgagcc actgaacttt
    12721 tgcggcggcc tgtgccgtag tagtatttac cgttcatgtc gcgtccttat ttcagttcca
    12781 aaactttggg ttgttgcgca gcatgggcgt gttccgcacc cgcatacact ttcagttttt
    12841 taatcatggc gtaacccaaa ggacctttgg gcagcatacc ttttacagct tgttccaaag
    12901 cgcggcccgg gaattgctct tgcatttcgc ggaaagtgcg ttcgtagata ccgcctggga
    12961 aaccggaatg gcggaagtat tttttatctt cgaatttggc accggttaca cgcagtttgt
    13021 ccgcattgat aacaatgatg taatcgccgg tatcgacgtg gggggtgtat tcaggtttgt
    13081 gtttgccacg cagacggctg gcgacttcgg ccgcaacgcg acccaagact ttgtcttggg
    13141 catcgatgac gaaccattcg cgcttcacct cgtggggttt cgctgaaaag gttttcatag
    13201 tggaaatcca aatagatata gaaagttgta aattttaaag acaggattcg attttgtcaa
    13261 tcgcattacc gcgttacgga aggattttcc ggatttcggc agactgcata ctgctttttc
    13321 gggcggggcg gcggccaatg tgaaaaaccg catcgttgcg atgcggtttt gaatgggaat
    13381 ccccgcgaga gccgtttcgg ccgaatccgc ttgaaccttg ctgacaaggc ggctgcctcg
    13441 ggtagtttcg ggtgcgtccg caaaaggacg ctcgcgccca ctactgctcc cggcaacctt
    13501 aagcgaactt attggttcaa aggaatatat gccttcgcgg acaccgcagg gaaaaagggg
    13561 ttattcctgc gccaagcggg atagtgcttt ttggcaggcg ttgtccatat cggctatttt
    13621 acgcgcaaaa tcgccgattg ccaaatcgcc gccgttcagg gaggttttca acaggtcgtg
    13681 gacgacgttg agcgcggcca taatgacgat tttttcgctg tccgcgacgc gtccgccttc
    13741 gcggatggct tcggctttgc cgttgagcat tccgactgcc tgcaacagtg tgtctttttc
    13801 ttctgccggc gtgttgacgg tcagccgggc gtgcatgact tcgatgtgga cttgttcgat
    13861 gttcatcctt taatccttat tgctgcgttt cctgccattg ggggaggcgc gctgccagtg
    13921 cgctgatttt ttccctgctc tgttcgagca ggctgcggta tcgtgtattt tcttctgtca
    13981 ggctgtcgat tttgttttgc aggtcttctt tgagtttgcc gacttggacg agcagggctt
    14041 cgctgagttc gtcgacggcg gtttcgtgtt cgagtttttg ccgctcgtgc gcccgtttga
    14101 gttcggcgac ggtttctttg aggcggcggt tttcgctgac gagggtttcg aatttttgta
    14161 ccagcgtata aacgctgctt tcgagttttt cgatattttg tttcataacc ttacctgtcc
    14221 gtatgccgtc tgaaggcttc agacggcatc tgtctgttgt ttattcaaaa cgcgcgctgc
    14281 gttccatcag cctttcgacg acctgttgcg gggtcatttc tttgcggatg agttgcagca
    14341 gggtttgggt aatcggcatg tcgatttggt acttacaggc agtattgaag acttcttcta
    14401 tcgtgctgac cccttcggaa acgtgtccga tttcgaccag cacctgatgc agttccttgc
    14461 cttctgccaa acccaagccg acgcggcggt tgcgtgagag tgcgccggtg caggtcaaga
    14521 tgaggtcgcc gatgcctgcc agccccatca tggttttggg ctgtgcgccc attgcggagg
    14581 caaggcgggt gatttcagct aatccgcgcg taaccagtgc ggcacgggcg ttaagcccgt
    14641 actctagacc gtcggacaat ccggtggcaa tcgccataac attttttacc gcgccgccga
    14701 ctgccacgcc gataacatcg gtactgccgt aaagcctcat gacggtcgtg ttgagctgcg
    14761 gtacgagttc ttcaatccac tcttggtttt cggaggcgag gacgacggcg cagggcagtt
    14821 gtttggcgag ttcctgtgca aaactcggac cggaaagtac gccgattttt ttattgtcgg
    14881 gcaatacttc tttcaatact tgaaaggtca gcagccctgt atcctgctcg aatcctttgc
    14941 aggcggcgag gacggggagg tgtcccgcgc cgtactgttt gagcagctct gcgctgcttc
    15001 tcaatccggc aacggaggtt acgataagga caagtccgct gtctttgagc gcgtctgcca
    15061 aatccgcaca cacttccaag gtttcgggaa aggaaaagcc gggtagtccg cgtttgtttt
    15121 cacgcgcttc ctgcatttgg cggacttggt ctgcgttgcg cgtccacagg gatacgcggt
    15181 tgccgtgttg ggaaaagtgc agggcgagcg ccgtacccca cgaacctgcg ccgataacgg
    15241 taattttcat tggtcgtctt tcaacatatc actgccgttc actttaaaac aatcggtgtt
    15301 tctctgcaag tgcggtcagg gaaatgccgt ctgaaaggtg ttcagacggc attttgcccc
    15361 gatgcggcac aatcagcctg tattgcgcaa accttgcgcc acgccgttga tggtcaggtg
    15421 aaccatcagc agcgcgtgcg gattgtcggg ttctttacgc aggcgtttga gcatggcgac
    15481 ctgcaggccg ttgagcgcgt tcaaatatgg aatcctcaag gcaagcgaac gggcgaggct
    15541 gcggttgtcg cgtaaaagtt cttcggtttg cagcaggtcg agcagggctt tgcggctgcg
    15601 ttggtattct tccttaatca tgccgaagat gacggcggct ttttccggcg attcgctcaa
    15661 gccggcatag ttttccgcga gggtgatgtc ggttttcgcc atcacttgct ccatattgga
    15721 aagcatggct tggaagaacg ggttgctttg ggcgtgttcg cgcagggcgg cgagcgtttc
    15781 gggtttgtct tcgcacaagg tttccaccgc gctgccgaaa ccgtaccaag caggcagcat
    15841 gaggcggttt tgcatccatg agaataccca cggaatcgca cgcaagtcct gaatccgcgc
    15901 caaggttttg cggctggcag gacggctgcc gaggttgagg gtggcgattt cctgaatcgg
    15961 gctggtttgc aggaagtagt cgatgaagtc ggggtgggta atcagttcgc ggtagtattt
    16021 gaacgatacg tccgacaatg cctgcatcag tttggcatca gggtcttttt tatccggcag
    16081 gatgctggct tccaaagtcg cggcaaccaa ggtttccaag ttgcgttggg cattgccggg
    16141 gtcggcgtat ttggcggtaa tgacttcgcc ttgttcggta atgcggattt gtcccgccac
    16201 gctgcccgcc ggttgggcga gaatggcttg gtaagaaggg ccgccgccgc gtccgacgct
    16261 gccgccgcga ccgtggaaca ggcgcatgcg gacatcgtat tttttgaaga gttcgaccaa
    16321 gcccaattcc gcctgataca gacaccatga gctggtaaca tagccgccgt ctttgttgga
    16381 gtcggagtag ccgagcataa tttcttggat gttgccgcag ctttcgagca gggcatcgta
    16441 ccaatcgagg cggaacatgg tttccatgac cggacaggcg ttttcgagtg cttcgatggt
    16501 ttcaaacagc ggcacgatat tgatgcggct gcgcggtttg ccgttttcca ccgccagcag
    16561 gccgctttct ttcagcagca atgccaaggc gagcaggtcg ctgggttgtt cgcagttgga
    16621 aataatgctt tgtgtaacgg catcttcgcc gaattcgtct ttgattttgc gcgcttcgtt
    16681 gaaaattgcc agttcgtggc gggtatggtc gctgtatgtg atgaacgggc tgtacagcgg
    16741 acgttgatgg ctcaattcgc gcaacagggc ggcttgtttt tgctcttcgt tcaggctgtt
    16801 gtagtcttcc aagcctgcgt gttggaaaag ctcggcaacc acatcggcgt gtttgcctgc
    16861 gtgttggcgc aggtcgagcg gcatcatgtg gaagccgaac acggacacgg aacggatgag
    16921 gtctgccaaa cggccttcag caagcaggcg gctgccgttg tcgataaggg aacgttgcaa
    16981 ttttttcaaa tcatcgagaa attcttgtgc cgaagcatac ggctcgagaa agccgaattt
    17041 gcagcccatg cccaaaccga gcgcgcgcgc tttgcccata gcgcgcgcca tgatgtaagc
    17101 aatggcgcgg cggtagggtt cttcggtgcg agcgatttct tcgtcaggcg agagggctgc
    17161 cagtgccatt acgtcgtcgt tgactttgac gcggcggatg gagagcggca gttcgcggta
    17221 aagtttgtcg agttcgccac ggtaaaaacg gaacacggca tcggcgtggc ggcggaaggc
    17281 aaaacgcaag gtttcggcag aaacgaacgg attgccgtcg cggtcgccac cgatccagcc
    17341 gccgattttg aggatgttcg gaacgcagac atcgggatag accatctgaa agtcgtgttc
    17401 catattgcgg tagagcttgg gcagggcttc gaaaaagctc atcgggaaga tggacacgcc
    17461 gttgttgatt tcgtcgttga cgctgagttt gtggcggcgc gtttcgctgg tctgccacaa
    17521 gcccagcagg atagtgtcga tttcgcggcg cagccgtgcc agcgcgtcgg cattggtgca
    17581 gcgttcgcgt tgcggcaaca gtgcgcggat gcggcggttg aagcttaaga cggtttggcg
    17641 ttgcacttcg gtcgggtgcg cggtcaaaac ggcggtaacg gacgtattgt ccaactgccg
    17701 ctgcaccgat ttgccgtcgg ctttccccgc tttgagcctg cggacggttt ccgtcaggct
    17761 gccttccgcg ccgccgcgtc cggcttcttc gtggatttgg cggcggcgtt cgtggtgcac
    17821 gtcttcggcg atattcaaaa tctgggcgaa caggccgcag gccaaggtta aatcgtgggt
    17881 ttgttgttcg tccaattgcg gcaatacttt ttcaatcaat gccgcgctgt cgtcggaagt
    17941 ggacaagagt ttgaccgttt cgacaaccaa tggcgaggct tcttcgtgca ggaggttgaa
    18001 cagggattgt ttcagaaatt ccgcgtccgc cgccaaagcc gcgtccttcg gattgttcag
    18061 aatatgcagt tgcatgattt ttctctcatt gccgtaaata ctgtaaatgt acctcaaatg
    18121 ccgcatccgt gccaaaccgt tcacacttta accgcccgtg tcccgaaatg ccgtctgaag
    18181 ttgaacgccg cccgacggca gcgttacaat cgcccacaac tgttttttcc gaacatcatc
    18241 atgaccacga ccgaacacga caacgacgat gcattcctgc tgcggtacag ccgccacatc
    18301 ctcttggacg aaattggcat cgaagggcag cagaaacttt ccgccgcgca tattttggtc
    18361 gtcggctgcg gcggtttggg tgccgccgcc ctgccctatc tcgccgcttc cggcatcggc
    18421 acgctgacca tagccgattc cgacacggtc gagctgcaca acctgcaacg ccaagtcgca
    18481 tttgacgagg gcgatgtcgg caagcccaaa gccgaagcct tggcagaccg cctgaaacgc
    18541 atcaaccata acgtcgatgt ccgcaccgtc aacgaaaaac tcgacggctg ccgcctgacc
    18601 ggtttggttc aaaccgccga catcgtttta gactgctgcg acaactacgc cacgcgccaa
    18661 gccgtcaacc gtgtctgcgt acaaacgaaa acaccgctgg tttcaggagc agcggtgcgc
    18721 tttgaagggc aacttgccgt gtaccgtccc gacttgcccg actcgccgtg ttacgcctgc
    18781 ctgtttgacg gcggatcggc ttcagacggc atctgttccc tcttcggcgt attctcgccg
    18841 ctggtcggca tcatcggctg cacgcaggcg gcggaggctc tgaaaatcct gctagacgtg
    18901 ggcgaaccgt cgcacggcag gctggcggtt taccgtgcct tggaaggggg ctggcaatat
    18961 ttcgacctgc cgcgcaaccc cgaatgcccg atttgcggca cggaacggta aaaccctgcc
    19021 gccgtttcag acggcataaa gacggatgcg gaggaacggt tttaaaaatc ggcggtgttt
    19081 tgacatagtg tcgataagca tacttatctt caatatactc aacaggataa ttgtttgcct
    19141 aagcaaaaaa taatcgcctt ttccctgacg ggcgggcgaa atccacggat tgcaaaaaca
    19201 caacctgaaa atttacattt ctttgcaaaa aaaaaaaaaa taaacttacc ttataattgc
    19261 agttgtctta gcaatgtctg tttcgcagac tcattgagta agacgttttc cccgtaatgt
    19321 gtttggccgt ctgtcccctt tgggttcgga cggctttttt ttggctgtgt atgaataccc
    19381 ggttggtttt atctgtttac aggggaaaac cgcttatttc cgttcgggcg gaaaacggtt
    19441 ccatcggata aaggcatttt gtccgactga ttaaag
//
LOCUS       DAWKST010000018        19422 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.018, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000018 DAWKST010000000
VERSION     DAWKST010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19422
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.018"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(143..349)
                     /locus_tag="WJL87_000442"
                     /pseudo
     CDS             complement(143..349)
                     /locus_tag="WJL87_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223883.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(392..1261)
                     /locus_tag="WJL87_000443"
     CDS             complement(392..1261)
                     /locus_tag="WJL87_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ7798923.1"
                     /translation="MKRIFLPALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENSRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLAGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDISAGISHTFLKDDKNPALIGFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPVVLSLTAAYRINGSKTLSSNTKYKAGNYWMLNPNISFAAN
                     DRISLTGGIQWLGKQPDRLDGKKESARNTSTYAHFGAGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            1425..2348
                     /locus_tag="WJL87_000444"
     CDS             1425..2348
                     /locus_tag="WJL87_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ7798924.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHTLFEPGKVMFYYRETSRESEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLLYPLDVVSRLVRGEKFFTTQEAEGV
                     PNAHTTVELIENRGGFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGLERVFYSRFCL"
     gene            complement(2426..3067)
                     /locus_tag="WJL87_000445"
     CDS             complement(2426..3067)
                     /locus_tag="WJL87_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ7798925.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSGGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTNRPEPSAVPYVAKTLMIHG
                     AEDEVVEIEKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(3122..3913)
                     /locus_tag="WJL87_000446"
     CDS             complement(3122..3913)
                     /locus_tag="WJL87_000446"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ7798926.1"
                     /translation="MARHPYRRLRPAKSSFPEVGISEEGNIRSLHLGSDTIQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDIILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLNVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNTYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            4079..4154
                     /locus_tag="WJL87_000447"
     tRNA            4079..4154
                     /locus_tag="WJL87_000447"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4112..4114,aa:Thr,seq:tgt)
     gene            4189..4264
                     /locus_tag="WJL87_000448"
     tRNA            4189..4264
                     /locus_tag="WJL87_000448"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4222..4224,aa:Thr,seq:tgt)
     gene            4406..5197
                     /gene="panB"
                     /locus_tag="WJL87_000449"
     CDS             4406..5197
                     /gene="panB"
                     /locus_tag="WJL87_000449"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ7798927.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVEMLLVGDS
                     LGMAVQGRKSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGSKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTESVSCPTIGIGAGVDCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMQGHNSVQAAVRAYVEEVKAKTFPAAEHIFTD"
     gene            5320..6156
                     /locus_tag="WJL87_000450"
     CDS             5320..6156
                     /locus_tag="WJL87_000450"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ7798928.1"
                     /translation="MQIIHTIRELRAWRKNAGKVAFVPTMGNLHEGHLALVREAKKRA
                     DSVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLEKRAVQSLTEYGWVVDYVEIRRADTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            6321..8159
                     /locus_tag="WJL87_000451"
     CDS             6321..8159
                     /locus_tag="WJL87_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798929.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVVKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTEEQRSRAALTAAMIYADRRDYAKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALRGLDKIIEKPPAGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            8299..8880
                     /locus_tag="WJL87_000452"
     CDS             8299..8880
                     /locus_tag="WJL87_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ7798930.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPLVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGIPSETETPERCAARTR"
     gene            8890..9735
                     /gene="ispE"
                     /locus_tag="WJL87_000453"
     CDS             8890..9735
                     /gene="ispE"
                     /locus_tag="WJL87_000453"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ7798931.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVDGMPQEADLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCSRTTQQLIDLGATLGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRDSAYNIYRQVSGLYEAYLAEGLSKH
                     PLLSV"
     gene            9743..9818
                     /locus_tag="WJL87_000454"
     tRNA            9743..9818
                     /locus_tag="WJL87_000454"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9776..9778,aa:Gln,seq:ttg)
     gene            9831..9906
                     /locus_tag="WJL87_000455"
     tRNA            9831..9906
                     /locus_tag="WJL87_000455"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9864..9866,aa:Gln,seq:ttg)
     gene            9915..9990
                     /locus_tag="WJL87_000456"
     tRNA            9915..9990
                     /locus_tag="WJL87_000456"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9948..9950,aa:Gln,seq:ttg)
     gene            10157..11140
                     /locus_tag="WJL87_000457"
     CDS             10157..11140
                     /locus_tag="WJL87_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ7798932.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            11207..11779
                     /locus_tag="WJL87_000458"
     CDS             11207..11779
                     /locus_tag="WJL87_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ7798933.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQPVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(11904..13073)
                     /locus_tag="WJL87_000459"
     CDS             complement(11904..13073)
                     /locus_tag="WJL87_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ7798934.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNKPAVHAAPTFQTPETL
                     TAAHIVIDLQSKQILSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPSDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLAQLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTIAEKEIVALENVKKRSRWQRLWARLTGQ"
     gene            13221..14747
                     /gene="ilvA"
                     /locus_tag="WJL87_000460"
     CDS             13221..14747
                     /gene="ilvA"
                     /locus_tag="WJL87_000460"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ7798935.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSHGGEVVLRGVSYNDAYDYAMELAEKEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGDETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(14804..15877)
                     /locus_tag="WJL87_000461"
     CDS             complement(14804..15877)
                     /locus_tag="WJL87_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ7798936.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGKILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKEQICTKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(15874..16734)
                     /gene="cysW"
                     /locus_tag="WJL87_000462"
     CDS             complement(15874..16734)
                     /gene="cysW"
                     /locus_tag="WJL87_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ7798937.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAV"
     gene            complement(17104..17940)
                     /gene="cysT"
                     /locus_tag="WJL87_000463"
     CDS             complement(17104..17940)
                     /gene="cysT"
                     /locus_tag="WJL87_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ7798938.1"
                     /translation="MLALKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     SGFWNTITEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            18079..18186
                     /locus_tag="WJL87_000464"
                     /pseudo
     CDS             18079..18186
                     /locus_tag="WJL87_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            18231..18563
                     /locus_tag="WJL87_000465"
     CDS             18231..18563
                     /locus_tag="WJL87_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ7798939.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            18894..19403
                     /locus_tag="WJL87_000466"
     CDS             18894..19403
                     /locus_tag="WJL87_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ7798940.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT         4571 a         4506 c         5492 g         4853 t
ORIGIN      
        1 taatccctgc accgcgcaac atcgcactgc tatatccgcc tcctatcata ccaccagcgg
       61 ttgcagccaa ggtacttctt gttgaagcaa attgccctgt tttaatttta gaaatgccgt
      121 gattagccca agcactaatt gcccctccca tcaaagcacc tgccacaata ggaagaaacg
      181 ccccctcagt ctccttcatc tccttttgag aaagctcagc caactgcatc ggcgcatctg
      241 cctgcgtgtg gaatacgtat ttttcaaatg cctgattgtc caatccgttt gccattgcgg
      301 gggcaatcat agacagcatc attacggctg aggtgatttg ttttttcata ataactcctt
      361 tggattacaa ggttggaaaa tcaaaacctg cttaaaatgt atgctgtacg ccaaatttca
      421 gttcggaact gctttgccct gaaacgttga aacgtgcgga tgcgtttaaa gccgtggttt
      481 tggtgaaacc gaaacctgcg ccgaaatggg cataggtgga tgtgtttctt gcggattctt
      541 ttttgccgtc cagacggtcg ggctgcttgc ccagccattg gatgccgccc gtgaggctga
      601 ttctgtcgtt ggcggcgaag gatatattgg gattcagcat ccagtaattg cctgctttgt
      661 atttggtgtt gcttgaaagg gttttgctgc cgttgatacg gtaggcagca gtcaatgaga
      721 ggacgacggg gtcgatggct ttgtaggtgg tggcaccgat gagccacgat tttcccgacg
      781 aggctttgtt gcgcgatttt tcgtaaaccg tgctttcaag aaagccgatt agggcggggt
      841 ttttgtcgtc tttgaggaag gtgtggctga tgccggcgga tatgtcggac atccgtttgt
      901 tgcgggtttt gccgttgccg tcgagtttgc gttcttcgtg ccacagatag ctgccgctgc
      961 cgtaaatgtc ggtattgccg gtcagtccgt agcgcaaacc gagcgtgccg gcgagcatat
     1021 cggtattgct gccgttttct tgaatttcgg tcggaatggg gataaacgag gttgcgccgg
     1081 tttgaatgta aaccggtgcg gcaagttcgg cgcggctgtt ttcgctgttc aggtaggtaa
     1141 gggaagtttc cagtttccat ttgcccttgt cggtcattat gtcttcaatc gtcaagggca
     1201 ggtcggcata agtggataaa ggcaggatgg cgggcaaggc gggcaaaaag atgcgcttca
     1261 tatttctcct attgatatta attcttattt actgataata atgaatatcc gataattttg
     1321 tctaaataaa tttttaaatc aaaaaaaacg atatgtttgt ttttgtcaat tatgtttcga
     1381 tatgccgtct gaaaagtttg tgaaaaacgg ttacaatccg ccacatgaaa aaacgcaata
     1441 atcctcttcc tctgttgaac ggtgtcaaac ccagttattt ggtgctgccg catgaaaagc
     1501 agttttacgg gctgccgctg ctgcattttc tgtgcatccg ctttcctttt gtgggcgcgg
     1561 acgattggcg taggcggttg aacagtggtt ttgtggtcgg ttcggatggt gcggcgttgg
     1621 atgaacatac tttgttcgag ccgggtaagg tgatgtttta ttatcgggaa accagtcgcg
     1681 agagcgagcc gcgtattccg tttgaagaaa agattttgca tattgatgaa catttgattg
     1741 tggtggacaa gccgcatttt ctgcccgtca tccccagcgg gcgttttttg agggaaacgc
     1801 tgctcacacg tctgcgcctg cgtcctgaat tgcagcattt gaatgttgag gacattacgc
     1861 cgctgcaccg cttggacaag gatacggcag gcgtgatgct gctgtcgcat aatcctgcca
     1921 cgcgcggagc ctatcagacg atgtttcaaa acaaaacggt atggaaaacg tatgaggcgc
     1981 ttgcgccgac aaggacggat ttgctgtatc cgctcgatgt ggtttcgcgt ttggtaaggg
     2041 gtgaaaaatt ttttacgacg caagaggcgg aaggcgtacc gaatgcgcac acgacggtcg
     2101 aactgattga aaacaggggg ggattcagcc tttaccgcct tacgccgcat accggcaaga
     2161 aacaccagtt gcgcgtacac atgatgggct tgggtatgcc gttgttgaat gacgcgctct
     2221 atcccgtgcc gtctgaagcg ggaagcgagg attatcggaa acctttgaag ctattggcaa
     2281 aaaagattgc gtttgcagat cctttgtcgg gtctggaaag ggtgttttac agtaggtttt
     2341 gcctataatg agagggaatg caaacccgta tcggcagatg ccgccgatac gggtttgata
     2401 ttatcgatgc cgtctgaaag gggttttatc cgttcaggca tacgggcgcg aagcggagga
     2461 tggtgtcgcg caggacgatg agtttgccgt ggaagaaatg cgtggaaccg gcgatggtaa
     2521 tgacgggcaa atcttgcggt tcggcccatt tcagggcttt ttcgatttcg acgacttcgt
     2581 cttccgcgcc gtgtatcatc agcgttttgg caacataggg aacggcggac ggctcggggc
     2641 ggttggtata gtggcagact gccgcgccga tgagcagtaa caggtcgggt atgcgcgctt
     2701 gtgcggcaaa tgtggcgaca taaccgccga aggagaagcc ggataaggca aattcggggg
     2761 cttcggggtg ttgggcgcgg gcataatcga tgacggcgag gcagtcttgc gtttcgccgc
     2821 gtccgtaatc atgtgtgcct ccgctgccgc ctacgccgcg aaggttgggc aggtagcagt
     2881 ggaagccgag tttgcttaag gctttggcgg cagtttggat aactttgttg gtgtttgttc
     2941 cgccctggag ggggttggga tgattgatga cggcaacacc gcgtgccggt acttgttcgg
     3001 acgggatatg gatggtttct aaaatgccgg caggaccgga tatgtgtatg gtttcgggtt
     3061 tcagcataat ggtttcctct tgattttccg catgttccga tgcagccgtg aagatagttg
     3121 cttaaaggtg gaaatgcttg ccgttgttgg ggttggatgc tttcaggccg gcgagcatac
     3181 ggtggaagtc caatccgtat gtgttgctca gtttgtcggc gcgtttcttg agtttgtcta
     3241 tgttttgctc tttggggctg ctttggaatg ccattaccgc gacattgccg tgactttcgg
     3301 caggaagttc caagacgcgc ccttcaaaga cgtttaacag gcgttcgatg aagcgttggt
     3361 agcgtttgtc gccgctccac cagttggtta cgaatatgcc gtctgaagag agtgcgtttc
     3421 ggcagtctcg gaaaaacggt tcttcaacca gcgcatcgat gatttgttcg ccgtcgaacc
     3481 cgtccaccaa gatgatatcg gtgttgtggc ggaagacttt gatatattcc gcaccgtctg
     3541 cttcaataat ttcaaatttc tcgccctcga aaggcaactc aaacaggttg cgggcaatgg
     3601 cgataacctg cgggttgatg tccacggcgg tttggcgcgt gtcgggcagg taggtgtcta
     3661 tccagcgtgc aaacgaaccg ccgcccaagc cgatttgggt gatatgctgc ggaagagcgt
     3721 cggtaaacag cagccatccc atcatggcac ggctgtaaga gagtaccagc tcggacgggt
     3781 ggtcgaggtt cattgagctt tggatggtgt cgctgcccaa gtgaagcgaa cggatattgc
     3841 cttcttcgga aatgccgact tcgggaaagc tggattttgc aggacgcagg cggcggtagg
     3901 gatgtcttgc catcggtgtg aacggtcggt ttgaaaggca gatattttat cagaatggcg
     3961 atggttgtcc tgcttcaaat taatttcttt tcaaataaat tacttattcg gatttgccgg
     4021 ggctttcgga taaattcctt gccaaggtgc ggcattgcct gcataattcg ctttctttgc
     4081 cggtatagct cagttggtag agcacctgac ttgtaatcag ggggtcccga gttcgactct
     4141 tggtgccggc accatttctg ctgatggtgt tgcaaaacat ttcagtaagc cggtatagct
     4201 cagttggtag agcacctgac ttgtaatcag ggggtcccga gttcgactct tggtgccggc
     4261 accagatttg acagtccgat tgtgtaaaac agtcgggctg ttttgcattt gcggcacggt
     4321 cggtggcagg gctgcgggca tttcactata atagcccgtt tcaaactgaa aaggccgtct
     4381 gaaaagggcg gggtaacaat atctgatgat tactgtgaac acactgcaaa aaatgaaggc
     4441 ggcgggcgaa aaaatcgcta tgctgaccgc ttacgaatcc agttttgccg cactgatgga
     4501 cgatgccggc gtggaaatgc tgctggtcgg cgactctttg ggtatggcgg ttcaagggcg
     4561 gaaatcgacg ctgcccgtca gcctgcgcga tatgtgctat cacaccgaat gtgtagcacg
     4621 cggtgcaaaa aatgcgatga ttgtcagcga tttgccgttt ggtgcatatc agcagagtaa
     4681 ggagcaggcg tttgccgccg ccgccgaact gatggctgcc ggcgcgcata tggttaaact
     4741 cgaaggcggt gtgtggatgg cggaaactac cgaattcttg caaatgcgcg gtattccggt
     4801 ttgtgcgcat atcggtctga cgccgcaatc agtgtttgcc tttggcggat ataaggttca
     4861 gggtcgcgga agtaaggcgc aggcgttgct taacgatgcc aaggcgcatg acgatgcggg
     4921 ggcggcggtc gtgttgatgg agtgtgttcc tgcggaactg gcaaaaaagg taactgaatc
     4981 cgtttcttgt ccgaccatcg gtatcggtgc gggtgtagat tgcgacgggc aggttttggt
     5041 aatgcacgat atgctcggta tttttccggg taagacggcg aaattcgtca aaaacttcat
     5101 gcaggggcat aatagcgttc aagcggcggt tcgtgcgtat gtcgaagaag ttaaagcaaa
     5161 aacctttcct gctgcagaac atatctttac agactgaacg gtttacatgc agcatgccgt
     5221 ctgaaagccg tttcagacgg cattttgttt tgccgtgcgc ggcgcgtata atcggcgcgt
     5281 tttgtcgggc aggaagcccg aaggataagg attaccgtta tgcaaatcat acataccatt
     5341 cgagaactgc gcgcgtggcg taaaaatgcg ggaaaggtgg catttgtgcc gaccatgggc
     5401 aatctgcatg aagggcatct tgcgcttgtg cgtgaggcga aaaaacgcgc ggacagtgtc
     5461 gtggtcagca ttttcgtcaa ccgcctgcaa ttcggtcagg gcgaggattt cgacaaatat
     5521 ccgcgtactt tgcaacagga tgcggacaag ctggaagccg agggtgttgc cgttgttttc
     5581 gcgcccgatg agaaagaact ctatccgaac gtggaacagc gttacaacgt cgaaccgccc
     5641 aatctgcaaa atgagttgtg cggcaagttt cgtccgggac atttccgcgg ggtggcgacg
     5701 gtggtgtcca agctgttcaa tattgtcgcg ccggatgttg cctgtttcgg caaaaaagac
     5761 tatcagcagc ttgccgtgat taaaggtttg accgaggatt tgaattttga tgttgaaata
     5821 gtgcctgtcg atacggggcg cgcggaagac ggtttggcac tctccagccg caaccagtat
     5881 ttgagtgcgg cggaacgcga cgaagcaccg cgcctgtacc gcgaattaaa ggctgttgcc
     5941 gaatccttgg cgcagggcag tttggattat gcaggtttgg aaaaacgtgc cgtccaatcc
     6001 ctgacagaat acggctgggt ggtcgattat gtcgaaatcc gccgcgccga tacgctcgaa
     6061 gtggcgcggg cgggagataa gaaactggtg gtcttggccg ccgcccgtct agggacgacg
     6121 cgcctgattg acaatttgga aataaaactc ccttaaactg caagcgtcgg gaatgccgtc
     6181 tgaagcggat ttgcgtttca gacagcattt atttttttga acggggtttc gcaaatctac
     6241 aaacgattct gcttgtgata aagttacgcc tgattatatg ccgtctgaag gttcggacgg
     6301 ctgtcggata aaggaagatt atgttacccg cccgtttcac cattttatct gtgctcgcgg
     6361 cagccctgct tgccgggcag gcgtatgccg ccggcgcggc ggatgcgaag ccgccgaagg
     6421 aagtcgtaaa ggttttcaga aagcagcagc gttacagcga ggaagaaatc aaaaacgaac
     6481 gcgcacggct tgcggcagtg ggcgagcggg ttaatcagat atttacgttg ctgggagggg
     6541 aaaccgcctt gcaaaagggg caggcgggaa cggctttggc aacctatatg ctgatgttgg
     6601 aacgcacaaa atcccccgaa gtcgccgaac gcgccttgga aatggccgtg tcgctgaacg
     6661 cgtttgaaca ggcggaaatg atttatcaga aatggcggca gattgagcct ataccgggta
     6721 aggcgcaaaa acgggcgggg tggctgcgga acgtgctgag ggaaagagga aatcagcatc
     6781 tggacggact ggaagaagtg ctggctcagg cggacgaagg acagaaccgc agggtgtttt
     6841 tattgttggc acaagccgcc gtgcaacagg acgggttggc gcaaaaagca tcgaaagcgg
     6901 ttcgccgcgc ggcgttgaaa tatgaacatc tgcccgaagc ggcggttgcc gatgtggtgt
     6961 tcagcgtaca ggggcgtgaa aaggaaaagg caatcggagc tttgcagcgt ttggcgaagc
     7021 tcgatacgga aatattgccc cccactttaa tgacgttgcg tctgactgca cgcaaatatc
     7081 ccgaaatact cgacggcttt ttcgagcaga cagacaccca aaacctttcg gccgtctggc
     7141 aggaaatgga aattatgaat ctggtttccc tgcacaggct ggatgatgcc tatgcgcgtt
     7201 tgaacgtgct gttggaacgc aatccgaatg cagacctgta tattcaggca gcgatattgg
     7261 cggcaaaccg aaaagaaggt gcttccgtta tcgacggcta cgccgaaaag gcatacggca
     7321 gggggacgga ggaacagcgg agcagggcgg cgctaacggc ggcgatgata tatgccgacc
     7381 gcagggatta cgccaaagtc aggcagtggc tgaaaaaagt atccgcgccg gaatacctgt
     7441 tcgacaaagg tgtgctggcg gctgcggcgg ctgtcgagtt ggacggcggc agggcggctt
     7501 tgcggcagat cggcagggtg cggaaacttc ccgaacagca ggggcggtat tttacggcag
     7561 acaatttgtc caaaatacag atgctcgccc tgtcgaagct gcccgataaa cgggaggctt
     7621 tgagggggtt ggacaagatt atcgaaaaac cgcctgccgg cagtaataca gagttacagg
     7681 cagaggcatt ggtacagcgg tcagttgttt acgatcggct tggcaagcgg aaaaaaatga
     7741 tttcagatct tgaaagggcg ttcaggcttg cacccgataa cgctcagatt atgaataatc
     7801 tgggctacag cctgctttcc gattccaaac gtttggacga aggcttcgcc ctgcttcaga
     7861 cggcatacca aatcaacccg gacgataccg ctgtcaacga cagcataggc tgggcgtatt
     7921 acctgaaagg cgacgcggaa agcgcgctgc cgtatctgcg gtattcgttt gaaaacgacc
     7981 ccgagcccga agttgccgcc catttgggcg aagtgttgtg ggcattgggc gaacgcgatc
     8041 aggcggttga cgtatggacg caggcggcac accttacggg agacaagaaa atatggcggg
     8101 aaacgctcaa acgtcacggc atcgcattgc cccaaccttc ccgcaaaccc cggaaataat
     8161 gcaggtccat cctttcagac ggcataaggt ttgccgggaa gctgcggcat tcgggcaaac
     8221 cgcacgcagt tcgcacgcgt tttgcacggc acgccgaacc cgccggccgg caggatggca
     8281 cccgttaagg aaattctgat gaaacacacc gtatccgcat cggtcatcct gcttttgacc
     8341 gcttgtgcgc aattacctca aaataacgaa aacctgtggc agccgtccga acacatcagc
     8401 agttttgcag cagaagggcg gttggcagtg aaagcggaag ggaaaggttc gtatgcaaat
     8461 ttcgattgga cataccaacc gctcgtggaa accatcaata tcaatacccc tttgggcagt
     8521 acgctcgggc agttgtgtca agacagggac ggcgcattgg cagtggacgg caaaggaaat
     8581 gtctatcagg cggaaagtgc ggaagaattg agcaggcagc tggtcggttt caaactgcca
     8641 atccaatatc tgcatatctg ggcagatggc aggcgtgtgg cgggcgcgcc ttaccgcatc
     8701 ctgccggacg gcatattgga acaatacggt tggactgtcg gcagaaccgc cgacagtggg
     8761 gggcaagtcc gaacgttgca actgaataac ggaaatttga acatcaggct ggttttcacc
     8821 gaaatcggta ttccgtctga aaccgaaacc ccggaacgct gtgcggcgcg cacgagataa
     8881 ggcggacaga tgaatattgc ggacggacgg caggcgtttc ccgcacctgc aaaactgaat
     8941 ctcgatttga ggattaccgg caggcgggaa gacggttatc acaatatcga aagcatattc
     9001 tgcttgatag atttgcagga taccgtatat ttgaaaccga gggacgacgg caaaatcatc
     9061 ctgcacaatc cggttgatgg catgccgcag gaagccgatt tgagctaccg tgccgcatcg
     9121 ttgctgcaaa aatatgcgcg caaccccgcc ggcgtggaaa tatggctgga caaaaaaatc
     9181 ccgacagggg cgggtttggg cggcggaagc tcggatgcgg caaccgtttt gctggtgttg
     9241 aaccgttggt ggcagtgttc ccgaacgacg cagcagctga ttgacttggg cgcaactttg
     9301 ggtgcggacg taccattttt tattttcgga aaaaatgctt ttgcaagcgg gataggtgaa
     9361 aaactgatag gaatggatat tccgaaacag tggtatgtca tcgtcaaacc gcccgtccac
     9421 gtttccactg caaaaatttt cacacacgaa ggcttgacac gaaattccgc ctcaagcata
     9481 atgccgactt tccaaaacct gcaaccgttt agaaatgata tgcaggcagt tgtatttaaa
     9541 gaataccctg aagtttggaa agcctattcc gaattgtccc gatatggatt tgccttaatg
     9601 acaggttctg gtgcgtgtgt attcacggcg tgtcaagata gggatagcgc atacaatata
     9661 taccgacaag tttcaggttt gtacgaggca tatttggcag agggtctttc aaaacatcct
     9721 ttgttgtccg tataaacatt gttggggagt cgtcaagcgg ttaagacact ggattttgat
     9781 tccagcatgc gaaggttcga atccttcctc cccagccaag ccaaacgagt tggggagtcg
     9841 tcaagcggtt aagacactgg attttgattc cagcatgcga aggttcgaat ccttcctccc
     9901 cagccagccc tttttgggga gtcgtcaagc ggttaagaca ctggattttg attccagcat
     9961 gcgaaggttc gaatccttcc tccccagcca aataaaagcg tgtaagcctg cttacacgct
    10021 tttatttcat agaaataaaa atattgaaat gcctttgttt gtgtcggatg ttgcaggtat
    10081 aatgtcgggc ttggtacaag cagagggaag cattgtgttt tctgagcgga agttaaacat
    10141 aaaatcaggt gagaatatgg ctgcgtacga cagtttgatg gtatttacag gcaatgccaa
    10201 tcccgaattg gcacaacgtg ttgtcaggca tttggacatt tctttgggca atgcttccgt
    10261 atccaagttt tcagacggcg aagttgccgt cgaactgttg gaaaacgtac gcgggcgcga
    10321 tgttttcatc cttcagccga cctgtgcgcc gaccaatgac aacctgatgg aaatcctgac
    10381 gatggcggat gcactgaagc gtgcttcggc aggtcgtatt accacagcca ttccgtattt
    10441 cggctatgcg cgccaagacc gccgtccgcg ttccgtccgc gttccgattt ctgccaaact
    10501 ggtggcaaat atgctgtatt cggcagggat cgaccgtgtt ttgactgtcg atttgcatgc
    10561 cgaccagatt caaggtttct tcgatattcc ggtggacaat atttatgcca ccccgattct
    10621 gttgaacgac atcaaacaac agcggattga aaatctgacc gtcgtcagcc cggacatcgg
    10681 cggtgtcgtc cgcgcccgcg ccgtggcaaa atccctgaat gccgacttgg caatcatcga
    10741 caaacgccgc ccgaaagcca atgtggcgga agtcatgaac atcatcggcg atattcaagg
    10801 tagaacctgt ctgattgtgg acgatatgat tgacactgca aatacgctgt gcaaagccgc
    10861 cgtcgccctg aaagagcggg gggctgaacg tgttctagcc tatgccagcc acgccgtatt
    10921 ctccggagag gcggtcagcc gtatcgcctc atccgaaatc gaccaggtgg tcgtaaccga
    10981 taccattcct ttgtctgaag cggctaaaaa ctgcgaccgt atccgtcagg taacgattgc
    11041 cggtctgttg gccgaaaccg tccgccgcat tagcaatgaa gaatccgtct catatctttt
    11101 caatgaagaa gtgatgacag gcagcatgtt gctgccataa gcccgaagcc gtcttaagct
    11161 ggtcgcggcc gatgacggcg attttaccta acttggagta tttaacatga cttatgaaat
    11221 tcaagcctct gttcgtgaag cacaaggcac tggtgcgagc cgccgcctgc gtcgcgaagg
    11281 ccaaatcccc ggcattctgt acggtgaagg tcaagagcct gttgcaatcg ctgtggatca
    11341 caaaaccgta ttctacgcat tggaaaaaga atctttccat actgcgttga ttaagttgtc
    11401 tctgaacggt gaaaccaaag acgttatcgt ccgcgatttc caaatgcacc cgttccgccg
    11461 cgaagttcaa cacatcgact tccaagctgt gaaagcagac caacccgtac gcatccgcgt
    11521 tcccctgcac atcgttaacg ctgaaaattc ccaagcggtc aaactgcaag gcggccgcgt
    11581 atctctgtta aacacttctg ttgaagtagt tgctttgcct gccaacatcc ctgctttctt
    11641 ggatttggat tgtgctgaag tggttgccgg cgacattctg cacttgtcag acatcaaact
    11701 gcctgaaggt gtagaaagcg tttccctgaa acgtaacgaa aatctggctg ttgctaccgt
    11761 taccggtaag aaacgctaat tgatttcagc agcagggcgg cgcgtatgca atacgtaccg
    11821 ccctgttgtt ttatgccgtc tgaaccgtgt ttcagacggc atttctttat ttgttggaaa
    11881 aacgggatat ttgaaatagc agattactgc cctgtcagac gcgcccaaag cctttgccac
    11941 cggcttcttt tttttacatt ttccagtgcg acgatttctt tttcggcaat ggtgtatccg
    12001 ttttgtctga ttttgatttt tcctaaaatt tgcccttttt ttaccggggc gggaatcggc
    12061 tgtatggttt ccaaaatctg ttctgccatt ttcgcttcct tatgtggcag agtgatgtag
    12121 gcttctttga ggaagcctgc gcggacggtt tttttgctgc ctccggaaat ttggatttgg
    12181 gcaacggttt tgcctttcgg atatattttg ggcgtatcga aggcctgcaa tgcccagttc
    12241 agcagcttgc tgttgtctga tgcgcgtgtt tccgccgatt ccgaacccaa tgtgatgaca
    12301 aggatgtgcc tgccgttgcc ggagtatgac acggcaaggt tgtagccgcc gctttctgtg
    12361 tgtccggctt tcagaccgtt tacattgttg tccctatata aaaggatatt gcggttgttt
    12421 tgctctatat ttttgaattt gaaagatttg atggaaaaca gcgggtaata ttccggaaag
    12481 tcgcgcatca atgcttcaga cagctgggcg aggtctttgg cggtggaaac ctgtccttct
    12541 ctactcaagc ctgtcggatt tttgaataca gtgttcttca tgcccaagcg tcgggcttct
    12601 ttgttcattt gttgcacaaa attttcaatc gagccgttgc ccagccggcc ggcaagggtt
    12661 agggcggcat cgtttgcgga tagtgcaatc atgcctttta agagtttgtc ggtgctgacc
    12721 gtatcgctgg gacgtacaaa cattctgctt ccttctgaag cccatgcgga ttcgggtatt
    12781 tttaagtttt cttcagatcg gatatttccc gatttcatgt ttttgaaaac cagatatgcg
    12841 gtcatcagtt gggttagtgc cgccggttcg acaggggtat tgatgttttt ggcggataaa
    12901 atctgtttgc tttgaaggtc gataacgatg tgtgccgctg tgagggtttc gggtgtttgg
    12961 aacgtggggg cggcgtgtac cgccggtttg ttgggcgcgg gcgatgcggc cgttgcgtga
    13021 gaaacgccta agatgatgga aagcaggacg ggcaggattt tatgtgctgt catgaaatat
    13081 tctaattgtg tgcgtgtttc agtctgccga ttatacgctt agggtgtctg atcgggcgga
    13141 tttttcttgc tttcgcgccg tcttgggcgt atggttttgg gttttgcgat tttaataaac
    13201 cgattatccc atattgaatt atgaacacgc cccttcctta ttccaattac ctcatccgca
    13261 tcctgacggc atctgtctat gatgtcgcgg tcgaaacgcc tttggaaccg gcacgcagcc
    13321 tttctgtacg tttgaaaaac aacatccttt tgaaacgcga agatttgcag ccggtttttt
    13381 cgttcaaaat acgcggcgcg tacaacaaaa tgtccaagtt gccgaaagat gcgctcgctt
    13441 gcggcgtgat tgcggcaagc gcgggcaatc atgctcaagg cgtggcattg tccgcacagc
    13501 gtttgggttg ccgtgccgtt atcgttatgc cggaaactac gccgaaaatc aaagtggatg
    13561 cggttaaaag ccatggcggc gaggtggttt tgcggggcgt ttcatacaac gatgcctacg
    13621 attatgcgat ggagttggcg gaaaaagaag ggttaaccta tatcgcgccg tttgatgatc
    13681 ctgatgtgat tgcgggacag gggacggtgg ggatggaaat tgtcagccag catcccgatc
    13741 caatccgcgc cgtattcgta ccgataggcg gtggcggttt ggcggcgggc gtggcggcat
    13801 ttatcaagca ggtccgcccc gaaatcaaag ttatcggcgt tcagaccaac gattcctgct
    13861 gtatgaagca gtcggtcgaa gcgggtgaaa tcgtccattt gaaagatgtc gggctgtttt
    13921 cagacggcac tgcggtcaaa gttgtcggag acgaaacctt ccgcctctgc aaagaacttt
    13981 tggatgaaat cattacagtc gataccgatg cggtttgcgg cgcggtcaag gatattttcg
    14041 atgacacgcg cagcattacc gagccggcgg gcgcgttggc gttggcgggt ctgaaagcct
    14101 atatcgcccg agaaggcgcg gaaaaccaaa ccctgattgc cgttaccagc ggtgcgaata
    14161 tgaattttca ccgtttgcgc catgtgtcgg agcgtagtga attgggcgag ggcaacgaag
    14221 gtatttttgc cgttaccatc cccgaagaac gcggcagctt ccttaagttt gtcaatatat
    14281 tgggaaatag gaatattacc gagttcaact accgctacgg agacgatgaa aaagcgcata
    14341 tctttgtcgg acttcaagcg gcaggcccgc aggatttggc ggttatcggc agccggttgg
    14401 atgaggcggg attgcccaat gtcgacctga ctgatgatga gattgccaaa atccatatcc
    14461 gttatatggt tggagggcgg acggacaaag tagaaaacga gcgtttggtc agttttgagt
    14521 ttccggagcg tccgggggcg ttggcgcgtt ttttgaacca tatgcagggc ggttggaaca
    14581 ttacgctctt ccattaccgc aaccacggtg cggattacgg gcggattttg gtcggtatcg
    14641 atgtgccgcc gcacgatgcc gccgcatttg acggtttctt ggaaagtctg ggatacagct
    14701 atcacgagga aacgcaaaat gccgcgtaca agctgtttct tgcctgacgc ttgaaagcac
    14761 aatgccgtct gaaagccttt cagacggcat tgcgctttca tggttaaatc gaatattcaa
    14821 tcagttcgtt ttgagagaag acataaacct gtttcggaat cagcttcaat tctttgcctt
    14881 cggcgattgg gtaacgcgcg gcatcgctgc cagccagcgt gatatgcacg tcttgtttgc
    14941 cgtgtttcac caaaatatgc gtcaatgccc cgacggcgtg gactttttcg atttcggcac
    15001 aaatcatcgg tgtttcgtgt tcggcggcga tctgccattc gtgcgggcgg atatagccgg
    15061 tggcggtttg ttcctgccat ttgtattgtg cgtccaattt ccacgcgaag ccgttataat
    15121 gccagaagcc tttttcgatg cgtccttcaa aagcgtcggt ttcgccgagg aactcggtaa
    15181 cgaaggcatt ttcgggtttg cggtaaatag cttcggcgct gccggtttgt tcgattttgc
    15241 cgtggttcat cacgacgatt tcgtcggaaa cttcgagggc ttcttcttgg tcgtgcgtaa
    15301 ccagaatgct ggttacaccc aggttgtgat ggatatcgcg cagccaggtg cgtaattctt
    15361 tgcgtacttt ggcgtccaac gcgccgaagg gttcgtccaa aagcaggagt ttcggttcga
    15421 ccgcaagcgc acgggcgagg gcgatgcgct ggcgttgccc gccggagagt tggtgcggat
    15481 aggattttgc caaatgcgag agttgtacga gtttcagcaa ttcttcgact ttagtgcaga
    15541 tttgttcttt ggacgggcgt tcggacttgg gcaatacggt caaaccgaag gcaacgttgt
    15601 caaacacgtt catatggcgg aaaagggcgt agtgttggaa cacgaagccg actttgcgct
    15661 cgcgcacgtg cttggcggtt acgtcttgcc catcaaacag aatcttgccg ccgtcggcgt
    15721 tttccagtcc ggcgataatg cgtaaaagcg tggttttgcc gcagccggac gggccgagca
    15781 gggaaacgag tttgccggtg gggacgttga ggttgatgtt tttcagcgcg tgaaaattgc
    15841 cgaagtgttt gtttaagttt tggatggtga tactcatact gcgttccttt cggcggcggc
    15901 gagttttttg tcttgtaatt tggtgatgat gttctgcacc gccagcgtcg ccagtgccaa
    15961 aagtgccaat acgccggaga gggcgaatgc gccggtgaag ttgtattcgt tgtagaagat
    16021 ttcgaccaaa agcgggacgg tgttggtttc gccgcgtatg tgtcccgata ccacgctgac
    16081 cgcgccgaac tcgcccatcg cgcgggcgtt ggtgaggatg atgccgtaga gtaacgccca
    16141 tttgatgttg ggcagggtaa cgcgccaaaa catctgccag ccgcttgcgc cgagtatcaa
    16201 tgccgcctgt tcttcgctgt cgccctgagc ctgcatcagc gggatgattt cgcgtgcgac
    16261 aaaggggaaa gtaacgaaca gcgtcgccag aataatgccg gggatggcga agataatctg
    16321 tatgccttgc gcttcgagcc agccacccaa tgccgtgtgc gcgccgaaca ataagacgaa
    16381 cattaaaccg gccaccacgg gcgatacgga aaacggcaaa tcgagcaggg tagtcagcaa
    16441 ctgcttgccg cgaaaatcaa aacgggtcag cagccacgcc atcgccacac ccaatacagc
    16501 attgacggga acgacaatca gcgcggtaat cagcgtcaat ttgatggcag accacgcttc
    16561 gggatcgttt aaggatttta ggtacaaatc ccaaccgcct tttaaggctt cgtaaaacac
    16621 ggcgacgagc ggcacgacca gcatcagcag cagaaagccc agcgcggcgg caatcagcaa
    16681 cacgcgcagc cggcgcggtt cggtcaggtt gggattggcg gaatagggtt tcatggcggt
    16741 ggtgttctct ggttggttta aaatgtttgg aggtcgtctg aaaagggatt gaggggtttc
    16801 agacgacctt tttgttcatt aagtagtagg tcggatactt gtatccgaca aggttgctga
    16861 atcgcaaatg tttgtcggat acaagtatcc gacctacgaa ttctgtgttt tgtggggcgg
    16921 attcgttttc agacgacctt ttattgatta agggaataaa aaaacgtcta gctgccgtca
    16981 ttcccgcgca ggcgggaatc caccacaggg caacaggaaa acagaaaata aataaggcag
    17041 ccgaaattca ccaatggatt cccgcctgcg cgggaatgac ggtaacaggt atttcagacg
    17101 acctcaaccc ttcgcgcccg aacgcctgcc caacgcccac tgcatcacgt tcagcgcaaa
    17161 cagaatcaca aacgaaacca gcagcataaa caacgccacc gccgacgcgc cctgtacgtc
    17221 gaactgttcc agcttgcccg taataatcag cggcaggatt tcagaaacca tcggaatatt
    17281 gcccgcgata aaaatcaccg agccgtattc ccccgttgcc cgcgcgaaca tcattcccgc
    17341 gccagtcaag agtgccggcg tgatttccgg cagcagcacg cggcggaacg tcgtaaagcg
    17401 gtttgcgccc aaagtcgccg ccgcttcctc atattcgccc gataattcct ccaacaccgg
    17461 ctgcacggcg cggacgataa agggcaggct gacgacgacc agcgcaatcc aaatgccgac
    17521 gggtgtaaac gcgattttga tgcccaaagg ctcgaaaaaa cggcctatcc aaccgttggg
    17581 cgcatacagg gtcgccaacg cgatacccgt aaccgccgtc ggcagcgcaa acggcaaatc
    17641 aaccagcgcg ttcgccaagc ccttgcccgg aaattcatag cgcaccaata cccacgccac
    17701 cagcgtgccg aacacgacat tggtcagcat cgcataaaac gacatccgca aactcagcca
    17761 gacggccgcc aagacattgg gttcggtaat cgtgttccaa aatccgctcc agccgatttc
    17821 cgccgccttc gccgccatca tcgcaaacgg caagaccaca agcagcgaca ggcacaatac
    17881 ggtcagacca aggctgagtt tgaagccggg cagtacgccg ggcgttttga gcgctaacat
    17941 aaaacaatgc tgaaaataag gaaaaggaag gactacttta acgatgccgt ccgaaaaacg
    18001 gaaagaatgg aaagtttggt gtaaagacga atttgttata aagcggttgg cagtttctca
    18061 agcgggcgcg atgttttaaa atatagtgga ttaaatttaa atcaggataa ggcgacgaag
    18121 ccgcagacag tacaaatagt acggcaaggc gaggcaacgc cgtactggtt taaatttaat
    18181 ccactataac gccttgtttt gacggaaaac catcatataa aggaacactt atgcagattt
    18241 tatcttttca gccggacatt gcggaacgta tgctggaagg tacggaaggc gagtcggtca
    18301 acgaaaacgc acaattcgtc cgtacggaca acggctattg gattgcgtgg catgaaggcg
    18361 tagcggcact gcttgcgccc gatacgccgc cgggcattcc ctgtttttgg gtggaaggcg
    18421 cggaaagcct tgaagagttg tgcgtcatgg tggaacgcgg cgagtttgac gaagtggaag
    18481 agtttgacgg cgatgacgac gaatggctcg aaacggcaca gggttgcggg caccacggcg
    18541 acgcttgcgc ctgcggacat taaaggcatt gcaggcttgc cgcaaggggg caaggctttg
    18601 ccgtttttta aataatcggt ggattgcatc tgtttgcaat gggctgaacg cttccctttg
    18661 tattttattg aataaaaaaa cttatcttgg tattataatt aaggcagcat caattatttt
    18721 gggatggcaa taaacgcaaa gcattgattt gcgccgattg cagacttatt atagcaggtt
    18781 gcggcgcgga cttaatggga attgtttata gagcatgatg gtctgaaaca tcattaatta
    18841 aaaataccat taatccgatt tatatttatt tcaatttcaa tggaaaaaca tcaatgacaa
    18901 tgattttaag cattttaagc ctgtttttta tcatcagact gttattttta gccgtctcta
    18961 ttaaacatga aaaagccttg attgccaaag gggcgaaaca atacggaaaa accaattcca
    19021 cgctgcttgc ggcagttcat acgctttatt atttggcgtg ttttgtttgg gtatggcttt
    19081 ctgacactgc ttttaatggc atatccttga ttggtacgct gacggtgatg gcttcgtttg
    19141 tgatattgtc attgattatt aagcagttgg gggagatttg gacggttaaa atctatattt
    19201 taccaaatca tcaaattaat cgttcgtggt tgtttaaaac attccgccac cccaattatt
    19261 ttttaaacat catacccgaa ctgattggca tcgccttatt atgtcaagcg tggtatgttt
    19321 tattgattgg cctgcccatt tatttgctgg tcttatttaa gcgtatccga caagaagaac
    19381 aggcgatggc aacacttttt taacccgttt catcaattat ag
//
LOCUS       DAWKST010000019        19314 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.019, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000019 DAWKST010000000
VERSION     DAWKST010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19314
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.019"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(265..423)
                     /locus_tag="WJL87_000467"
     CDS             complement(265..423)
                     /locus_tag="WJL87_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798941.1"
                     /translation="MKPPLPICTLSALAACTLSGQGGTQVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(420..1262)
                     /locus_tag="WJL87_000468"
     CDS             complement(420..1262)
                     /locus_tag="WJL87_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ7798942.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            1501..2421
                     /gene="pyrB"
                     /locus_tag="WJL87_000469"
     CDS             1501..2421
                     /gene="pyrB"
                     /locus_tag="WJL87_000469"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ7798943.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLDASMLARAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            2431..2889
                     /locus_tag="WJL87_000470"
     CDS             2431..2889
                     /locus_tag="WJL87_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ7798944.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            2954..3619
                     /locus_tag="WJL87_000471"
     CDS             2954..3619
                     /locus_tag="WJL87_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798945.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALDLGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(3682..4899)
                     /locus_tag="WJL87_000472"
     CDS             complement(3682..4899)
                     /locus_tag="WJL87_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ7798946.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFDRSE"
     gene            5041..5586
                     /gene="def"
                     /locus_tag="WJL87_000473"
     CDS             5041..5586
                     /gene="def"
                     /locus_tag="WJL87_000473"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ7798947.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            5673..6599
                     /locus_tag="WJL87_000474"
     CDS             5673..6599
                     /locus_tag="WJL87_000474"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ7798948.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            6678..7937
                     /gene="rsmB"
                     /locus_tag="WJL87_000475"
     CDS             6678..7937
                     /gene="rsmB"
                     /locus_tag="WJL87_000475"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ7798949.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAILRRFLRERDKLAASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNITAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIPARALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            7903..8502
                     /locus_tag="WJL87_000476"
     CDS             7903..8502
                     /locus_tag="WJL87_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ7798950.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            8502..10619
                     /locus_tag="WJL87_000477"
     CDS             8502..10619
                     /locus_tag="WJL87_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ7798951.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAIPVQIDLISTASLSGNMGRVLEHY
                     ANGFTQLSLYNAASGKIEKSINPHKLDQPFPGKARWEKIQQAGSVRDLESIGGVLYAQ
                     GWLLVGTYNGRDYALFFRQPIPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATLL
                     IASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTKL
                     FNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAAEQ
                     ILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKILL
                     GKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAERL
                     AWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALIG
                     DVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVKS
                     EAGQDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRIS
                     LSNQDAGGACVRIILPKTVKTYA"
     gene            10612..11889
                     /locus_tag="WJL87_000478"
     CDS             10612..11889
                     /locus_tag="WJL87_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ7798952.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QVRPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSAVENALKHGAAKIETGPVFDKLGNSAAIQEMNREVGAAVKRASPVL
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAEHRRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPAAFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAAAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            11980..13167
                     /gene="dprA"
                     /locus_tag="WJL87_000479"
     CDS             11980..13167
                     /gene="dprA"
                     /locus_tag="WJL87_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ7798953.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLLHHFGSAQNALSAPAEQV
                     AALIRHKQALEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLAQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRYATPQAMRIAKDFGRALGGKGIPVVSGMASGIDT
                     AAHQGALQAEGGTIAVWGTGIDRIYPPANKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLTECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            13193..13654
                     /locus_tag="WJL87_000480"
     CDS             13193..13654
                     /locus_tag="WJL87_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ7798954.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            13726..16032
                     /gene="topA"
                     /locus_tag="WJL87_000481"
     CDS             13726..16032
                     /gene="topA"
                     /locus_tag="WJL87_000481"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ7798955.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIAGGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAESCPKCAWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(16105..16800)
                     /locus_tag="WJL87_000482"
     CDS             complement(16105..16800)
                     /locus_tag="WJL87_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798956.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFAPEMN
                     RILSDFKTTRGFK"
     gene            17491..17868
                     /locus_tag="WJL87_000483"
     CDS             17491..17868
                     /locus_tag="WJL87_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7798957.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            17957..18526
                     /gene="rsmD"
                     /locus_tag="WJL87_000484"
     CDS             17957..18526
                     /gene="rsmD"
                     /locus_tag="WJL87_000484"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ7798958.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVCSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PDIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            18649..18900
                     /locus_tag="WJL87_000485"
     CDS             18649..18900
                     /locus_tag="WJL87_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ7798959.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            18997..19080
                     /locus_tag="WJL87_000486"
     tRNA            18997..19080
                     /locus_tag="WJL87_000486"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19031..19033,aa:Tyr,seq:gta)
     gene            19111..19184
                     /locus_tag="WJL87_000487"
     tRNA            19111..19184
                     /locus_tag="WJL87_000487"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19143..19145,aa:Gly,seq:tcc)
     gene            19194..19268
                     /locus_tag="WJL87_000488"
     tRNA            19194..19268
                     /locus_tag="WJL87_000488"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19226..19228,aa:Thr,seq:ggt)
BASE COUNT         4759 a         4969 c         5404 g         4182 t
ORIGIN      
        1 catttgtgtt tttgcatcat ttcgtttgat tttctagttt ttgagaatga tacaaagttt
       61 ttttacaaag taaagagtca ctctgaaaaa cattttttca ttataaatca aaatattgat
      121 agaataaata gcgagcatcg attcacggtg tactttagtg caaagcgtac aacgcgagcc
      181 tgaaccacca gccgcaacag gaaaagaaag ccgatagagt gcaatgcttg ccaacgtgca
      241 agcgagcttg caagaacgct tggctcaacg agagcaggca agacagaaag cagaaagcag
      301 gataggagcg gtaacgcaaa ggtctcgggc tttgatttcg ccgtaaacct gagtgccgcc
      361 ttgtccggaa agggtgcagg cggcgagtgc cgacagggtg cagatgggga gggggggttt
      421 catttggggt cgcaacggaa gtggtatgcg cagatttcaa aaccgttttt gaaatacagg
      481 cggtgcgcgt cggcacggtc gtggttgacg tggacgttga ggtggatttt ggttacccct
      541 gtttccgcgc cgattttgcg gacttcttcc aaaaggcgcg aggcgtagcc tttgcggcgg
      601 ctttgcggca gggtaacgat gtcatcgatg tggatgtggc ggccgctggc gagggtgcag
      661 gcttcgcgga agccgcagac ggcgacggca ttgtgtttgc cttcttcaaa aatacccagc
      721 aggcggtagc cttgggggcg ttggactttg ttgatctgtt cggtaaagcg gttgatgtcg
      781 gtcagggcgg aacgcaaaac gctcaaggct gcaaaggcgg tggcggtgtc gtccgcgccg
      841 atttcgcgca aaacgtagga tgcgcccgag gcggtctgtt cctgtgcttt ctcggcggcg
      901 tgtttttctt cgattgcctg tgccagcatg acgtgttcgt cggcagggtt gttttgtccg
      961 ccctgttcgc gttcttcgag cagggctttg cagtcgatga cgcgcaggtc gttgtcggcg
     1021 gcaaagtcca tcaggaagcg gaacatttgg gggttgtcgg tttccagttt tttatcgacg
     1081 gcgacgcagc ggatgttgtc caccagcatg gggcgcaccc atttcgaata ggaaagcctg
     1141 tggtctttgg tgaacgagga cagaatgccg cacaggcgtt ccgcccagtc gctgggacgg
     1201 aaaatcttgc cggaactcgt tgtgccgtgg atgacgactt cgtaggggtt gcagactaac
     1261 atggcggctt cctgaaaaga aatgtctggc gcgattatac cttatgctta tgcgggcgtg
     1321 tttggatatg ccgtctgaaa agtacgggat tcgtgcggta aaactttgcg gcggcaaatg
     1381 tgcgataata cgcgccgtat tgccgctttt gcgaagctgt tccgcaaaca tacgggcggc
     1441 gtggacgacg tataaccgga tacccgcctg acgcgggttt tttacggaag gggggcaaaa
     1501 atgcctaatc cgctttacag acagcatatc atctccattt cggatttgtc gcgcgaacag
     1561 ttggaatgcc tgcttcagac ggcattgaag ctgaaggcgc atccgcgcgg cgatttgttg
     1621 gaaggcaaac ttatcggttc gtgcttcttt gaaccgtcca cgcgcacgag gctgtcgttt
     1681 gaaacggcgg tgcagcggct gggcggcaag gtcatcggct tttcggacgg cgcgaatacc
     1741 agtgccaaaa aaggcgagac gcttgccgat accgcccgca tcatttccgg ctacaccgac
     1801 gcaatcatcc aacgccaccc caaagacggc gcggcgcgcg tggcagcgga gttttcgcgc
     1861 gtccccgtca tcaacgccgg cgacggcacc aaccagcacc ccagccagac cttgttggat
     1921 ctggttacca tttatgaaac gcagggacgt ttggacaagc tcaaaatcgc catggcgggc
     1981 gacttgaaat acggacgtac cgtgcattcg ctttgtcagg cgttgaaacg ctggggctgc
     2041 gaatttgcct ttgtttcgcc gcccagcctc gcgatgcccg actacattac cgaagagttg
     2101 gacgaagccg gctgccgata ccgtatcctc ggtagtttgg aagaagcggc ggaatgggcg
     2161 gacatcctgt atatgacccg tgttcaacgc gaacgtttcg acgaacagga atttgccaaa
     2221 atccaaggca aattcaatct cgacgcatcc atgcttgccc gcgccaaacc gaacctgcgc
     2281 gtgctgcacc ctttgccgcg cgtggacgaa atccatcccg atgtcgatgc tacgccgcat
     2341 gcctattatt tcgagcaggc gaccaacggc gtttatgcgc gtatggcgat attgtcgctg
     2401 gtgttgaacg aagaagtgtg aggaaccgat atggaaaccc cgaaactcag tgtcgaagcc
     2461 attgaaaaag gtacggttat cgaccatatt cccgccggca gggggctgac catcctgcgc
     2521 cagttcaaac ttttgcacta cggcaacgcg gtaaccgtgg gcttcaacct gcccagcaaa
     2581 acccaaggca gcaaagacat catcaaaatc aaaggcgtgt gcttggacga caaagccgcc
     2641 gaccgcctcg ccctgttcgc ccccgaagcg gtggtcaata ccatcgacaa tttcaaggtc
     2701 gtgcagaagc ggcatttgaa cctgcccgat gaaattgccg aagtgttccg ttgcccgaac
     2761 acgaattgcg ccggccacgg cgagccggtc aaaagccggt tttatgttaa aaaacacaac
     2821 gggcagacgc ggctgaaatg ccattactgc gaaaaaacct acagccggga ttcggtggcg
     2881 gaagcctgac ggattccctt aaaccgagcg ggcggcattt cgtctgccgc ttgttttgcc
     2941 aatctgaaat ggaatgatga tgcacgcttc tgtccaaagc cgtttcgcac cgatacttta
     3001 tgttttgatt ttctttgccg gttttttgac cgcgcaaatt tggttcaatc agaaagccta
     3061 tactgaagag ctgcctccgc ttctgtccgc attgtccgcc gtcgcgctgg tgtggctggc
     3121 gtgggcgttc gtgtcggcgc gttcaaaggc taaggcggaa aagttctacc gcgaaaaaat
     3181 gatacagaac gaaagcatac accccgtcct gcacgcttct ttgcaacact tggaacacaa
     3241 gccgcaaatg ctcgccctgc tggtcaaaaa ccacggcaaa gggatggcgg aacaggtcag
     3301 gttcaaggcg gaagtgctgc ccgacgacga agacgcgcgc acgattgccg ccgagttggc
     3361 aaaaatggat atgtttgcat tggggacgga cgcggtcgcc tcgggcgaaa cctatggacg
     3421 cgtgttcgcc gatattttcg agttgtcggc ggctttggaa gggcgcgcgt tcaaaggaat
     3481 gttgaaactg acggcggaat ataaaaacat cttcggcgat gcctgccgtt cggaaacggc
     3541 gttggatttg ggcgcgctca atcaggcgtt gcaggagatt tcaaaaacat cggaaaagtc
     3601 caaacggata ttttattgaa gatggaaaaa tgccgtctga aacggaaggt gtttcagacg
     3661 gcatttttgt cggatgatta attattcgga gcggtcgaag ccaaacttca cgcggctgcg
     3721 gccctgatcc ggtatattgt ccaaatcgcg tcccggattg gcggcggtgt cgcctacgga
     3781 aatatcggag atgttttcca aaatgatggc ggacgacagg tgttcggagg tgcggtaaac
     3841 cattgccaag cccacttctt cggcaggagt ggaaatcagc tcgacggtat ccctgctttt
     3901 gaaattgttg gagaggtcga cctgcatcgt tttcttgcgt ttgtagaggc tcaaaaccgt
     3961 gcctttgtcc aaaccgtccg cctcgccttt gtcgatggtg atggtttgaa actggccggc
     4021 aatccttgta ccttcaaaca cggaaacgat tttagcctga accgggcggg acggttcgtg
     4081 cggcatcatg ttgaagcggt cggtgtcttc cggcattttc atcaggtagt cgccctgctg
     4141 tatttcggaa atggcggttt cgaccaccag cggctgtatc gaaggggtgc gcagcggggt
     4201 aatcaaagga tgggtgcggg tatggtattc gttgtctttc ggccgttctc cagcctgttt
     4261 cgagcgttgt tcgaggacag agtcggtata gtcgagggag cgcacgatgc cgctgaatgc
     4321 gacttcctgc ccgaggaatt tacccgtatc cggatcggtg atgtttttat tgattcggta
     4381 ggtcaggtag cggcccggct ctttcaggcc tttggtgtaa accctggtgc ctttggtgta
     4441 cagcagcctg ccttccgggc ccgagagcag gcgcggcgcg gcagcggttt ctttgcggga
     4501 aacgatttgc gggtgccgca taaagatgcg gtagaagttg acatcgatgg cgggaatacc
     4561 gtatccggac acttccttat ccggactcat tttgacgacg gggatgccgt ctgtctgttc
     4621 caagccgagg cgcggttcgc cgtcaacgtg gcgcaacacc aatacctggt ccggataaat
     4681 caggtcggga ttgtggattt gatcccggtt cgcgtcccac aggcggcccc attgccacgg
     4741 gctgtacagg tatttgcccg aaatgcccca cagggtgtcg ccctgtttga ccgtgtagcg
     4801 ttccggcgcg ttcgggcgca cctccaaatt tgccgccaaa gtttgtgttg agaatgccat
     4861 acctgccgcg cagagcaggg ttataatacg acgttgcata accgttcccc ttatctgata
     4921 aatttcggtt tgtcttgctt gattgggttg gaaaaagcgg cggcagcccc tcgggatgtg
     4981 ccgcgtgata aaaaatgttc cgcattttaa catcgaatta tccgcaccat cacggtaatt
     5041 atgaaaaaca ggcggcgtat ccgccgaagg aaagagaaaa ttatggcttt attgaatatc
     5101 ttgcaatatc ccgacgagcg tctgcacacg gtggcaaagc ctgtcgaaca agtcgacgag
     5161 cgcatccgga agctgattgc cgatatgttt gaaacgatgt acgaatcgcg cggcatcggg
     5221 ctggcggcga cgcaggtcga tgtgcacgag cgcgtggtcg tgatggattt gaccgaagac
     5281 cgcagcgaac cgcgcgtgtt catcaacccc gtcatcgttg aaaaagacgg cgaaaccact
     5341 tacgaagagg gctgcctgtc cgtgccgggc atttacgaca ccgtaacccg cgccgaacgc
     5401 gtcaaggtcg aggctttgaa cgaaaaaggc gaaaagttca cgctggaggc ggacggcttg
     5461 ctggcgattt gcgtgcagca cgagttggac cacctgatgg gcatcgtgtt tgtcgaacgc
     5521 ctgtcgcagc tcaagcaggg acgcatcaaa accaagctga aaaaacgtca gaaacatacg
     5581 atttgaccct tttgccgtgc cgtctgaacg ctgcaaagtt ttcagacggc acggtcttgt
     5641 ccgacaacct tacgcatacg caggaatatg ctatgaaagt catctttgcc ggcacgcccg
     5701 attttgccgc cgccgcctta aaagccgttg ccgccgccgg ttttgaaatc ccgctggtgc
     5761 tgacccagcc cgaccgtccg aaagggcgcg gtatgcaact gaccgccccg cccgtcaaac
     5821 aagccgcgct ggaactcggt ttgcgcgtgg cacagcccga aaaactgcgt aacaacgccg
     5881 aagccctgca aatgctcaaa gaggtcgagg cagacgtaat ggtggttgcc gcctacggtt
     5941 tgattctgcc gcaggaagtg ttggatacgc cgaaacacgg ctgcctcaac atccacgctt
     6001 cgctgttacc ccgttggcgt ggcgcggcgc cgattcaacg cgcgattgaa gccggcgatg
     6061 ccgagacagg cgtgtgtatt atgcagatgg acatcggttt ggacaccggc gatgtggtca
     6121 gcgaacaccg ctacgccatc caaccgaccg ataccgccaa cgaagtccac gacgcgctga
     6181 tggaaatcgg tgcggcggcg gttgttgccg atttgcaaca gcttcaaagc aaaggccgtc
     6241 tgaacgcggt caaacagccc gaagaaggtg ttacttacgc ccaaaaacta agcaaagaag
     6301 aggcgcgtat cgattggagc gaaagcgcgg cggttatcga acgcaaaatc cgcgccttca
     6361 accccgtgcc tgccgcgtgg gttgagtatc agggcaagcc gatgaaaatc cggcgggcgg
     6421 aagtggtggc gcaacaaggc gcggcaggcg aagtgttgtc ctgttcggcg gacggtttgg
     6481 tcgttgcctg cggcgaaaac gcgctgaaga ttaccgaatt gcagcctgcc ggcggcaggc
     6541 gtatgaatat cgcggcgttt gcagcaggac ggcatatcga agcagggacg aagctgtaaa
     6601 tcccttcaga cggcattccg atccgcaaac gggaatgccg cctgaaacca tcagttgaag
     6661 aaagcgaatc acataatatg agtatggcac ttgcccaaaa acttgccgcc gacagcattg
     6721 cggcggttgc cgaaggacgc aaccttcagg acgtgttggc gcaaatccgc accgcgcatc
     6781 ccgaccttac ggcacaggaa aacggcgcgt tgcaggacat cgcctacggc tgccagcgtt
     6841 atttgggcag tttgaaacat atgctcgcgc agatgctgaa aaagccgatt gacaacccgc
     6901 agctcgaaag cctgcttttg gcggcgttgt accaactgca ttacacgcgc aacgcgccgc
     6961 acgccgtggt caacgaagcg gtggaaagca tagccaaaat cggacgcggg cagtaccgtt
     7021 cgtttgccaa cgcgattttg cgccgctttt tgcgcgaacg cgacaagctt gcggcttcct
     7081 gtaaaaaaga cgatgtggcg aaacacaacc tgccgctgtg gtgggtggct tacctgaaaa
     7141 accattatcc gaaacactgg cacaacatca ccgccgcgct acaatcccat ccgccgatga
     7201 cgttgcgcgt caaccgccga cacggcaatg ccgaaagcta tttggaaaaa ctggctgcgg
     7261 aaggtattcc ggccagggcg ttggacgaat atgcggttac gttggaagaa gccgtgccgg
     7321 taaaccgtct gcccggtttt tcagacggca ttgtttcggt acaggacttc ggtgcgcagc
     7381 aggcggcgta tcttcttaac ccgaaagacg gcgaacggat tttggacgcg tgcgccgcgc
     7441 cgggcggcaa gacggggcat atcttggaac tggcggattg ccgcgttacc gccttggaca
     7501 tcgatgcagg ccgtctgaag cgtgtggagg acaatatcgc ccgtttgggc tttcagacgg
     7561 catcggcggc gtgtgccgat gcgcgggatt tggcggcgtg gtatgatgga aagcctttcg
     7621 acgcaatcct tgccgacgtg ccatgcaccg cttccggcgt ggcgcggcgc aatcccgacg
     7681 tgaaatggct gcgccgtccg accgacgcgc tcaaaaccgc ccgccagcag gaagccctgc
     7741 tggacgcact ttggcagacg ctgaaacagg gcggcagaat gctgcttgcc acctgttccg
     7801 tgttcgtcga agaaaacgac gggcaattgc aaaaattcct caaccgccat gccgatgccg
     7861 aaccgatcga atcgcgggtg cttttaccga acaaacacca agatggcttt tattacgcgc
     7921 ttattcaaaa gcattaaaca atggcttgtg ctgctgccga tgctttccgt tttgccggac
     7981 gcggcggcgg aggggataga tgtgagccgc gccgaagcga ggataaccga cggcgggcag
     8041 ctttccatca gcagccgctt ccaaaccgag ctgcccgacc agctccaaca ggcgttgcgc
     8101 cggggcgtgc cgctcaactt taccttaagc tggcagcttt ccgccccgat aatcgcttct
     8161 tatcggttta aattggggca actgattggc gatgacgaca atattgacta caaactgagt
     8221 ttccatccgc tgaccaaccg ctaccgcgtt accgtcggcg cgttttcgac agactacgac
     8281 accttggatg cggcattgcg cgcgaccggc gcggttgcca actggaaagt cctgaacaaa
     8341 ggcgcgctgt ccggtgcgga agcaggggaa accaaggcgg aaatccgcct gacgctgtcc
     8401 acttcaaaac tgcccaagcc atttcaaatc aatgcattga cttctcaaaa ctggcatttg
     8461 gattcgggtt ggaaacctct aaacatcatc gggaacaaat aatgcgccgt tttctaccga
     8521 tcgcagccat atgcgccgtc gtcctgttgt acggactgac ggcggcaacc ggcagcacca
     8581 gttcgctggc ggattatttc tggtggatag ttgcgttcag cgcaatgctg ctgctggtgt
     8641 tgtccgccgt tttggcacgt tatgtcatat tgctgttgaa agacaggcgc gacggcgtat
     8701 tcggttcgca gattgccaaa cgcctttctg ggatgtttac gctggttgcc gtactgcccg
     8761 gcgtgtttct gttcggcgtt tccgcacagt tcatcaacgg tacgattaat tcgtggttcg
     8821 gcaacgatac ccacgaggcg cttgaacgca gcctcaattt gagcaagtcc gcattgaatc
     8881 tggcggcaga caacgccctt ggcaacgcca tccccgtgca gatagacctc atcagcaccg
     8941 cctccctatc cggcaatatg ggcagggtgc tggaacatta tgccaacggg tttacacagt
     9001 tgtcccttta caatgccgca agcggcaaaa tcgaaaaaag catcaacccg cacaagctcg
     9061 atcagccgtt tccaggtaag gcgcgttggg aaaaaatcca acaggcgggt tcggtcaggg
     9121 atttggaaag cataggcggc gtattgtacg cgcagggctg gctgctggtg ggtacataca
     9181 acggacgcga ttacgcgctg ttttttcgtc agcccattcc caaaggcgtg gcagaggatg
     9241 ccgtcttaat cgaaaaggca agggcgaaat atgctgagtt gagttacagc aaaaaaggtt
     9301 tgcagacctt tttcctggca accctgctga ttgcctcgct gctgtcgatt tttcttgcac
     9361 tggtcatggc actgtatttc gcccgccgtt tcgtcgaacc cgtcctatcg cttgccgagg
     9421 gggcaaaggc ggtggcgcaa ggcgatttca gccagacgcg ccccgtgttg cgcaacgacg
     9481 agttcggacg cttgaccaag ttgttcaacc acatgaccga gcagctttcc atcgccaaag
     9541 aagcagacga gcgcaacagg aggcgcgaag aagccgccag acattatctt gaatgcgtgt
     9601 tggaggggct gaccacgggc gtggtggtgt ttgacgaaca aggctgtctg aaaaccttca
     9661 acaaagcggc ggaacagatt ttggggatgc cgcttacccc cctgtggggc agcagccggc
     9721 acggttggca cggcgtttcg gcgcagcagt ccctgcttgc cgaagtgttt gccgccatcg
     9781 gcgcggcggc aggtacggac aaaccggtcc atgtgaaata tgccgcgccg gacgatgcca
     9841 aaatcctgct gggcaaggca accgtcctgc ccgaagacaa cggcaacggc gtggtaatgg
     9901 tgattgacga catcaccgtt ttgatacacg cgcaaaaaga agccgcgtgg ggcgaagtgg
     9961 ccaaacggct ggcacacgaa atccgcaatc cgctcacgcc catccagctt tccgccgaac
    10021 ggctggcgtg gaaattgggc gggaagctgg atgagcagga tgcgcaaatc ctgacgcgtt
    10081 cgaccgacac catcgtcaaa caggtggcgg cattgaagga aatggtcgaa gcattccgca
    10141 attatgcgcg ttccccttcg ctcaaattgg aaaatcagga tttgaacgcc ttaatcggcg
    10201 atgtgttggc attgtatgaa gccggtccgt gccggtttgc ggcggagctt gccggcgaac
    10261 cgctgatggt ggcggcggat acgaccgcca tgcggcaggt gctgcacaat attttcaaaa
    10321 atgccgccga agcggcggaa gaagccgatg tacccgaagt cagggtaaaa tcggaagcgg
    10381 ggcaggacgg tcggattgtc ctgacggttt gcgacaacgg caaagggttc ggcagggaaa
    10441 tgctgcacaa cgccttcgag ccgtatgtaa cggacaaacc ggcgggaacg ggattgggtc
    10501 tgcctgtggt gaaaaaaatc attgaagaac acggtggccg catcagcctg agcaatcagg
    10561 atgcgggtgg ggcgtgtgtc agaatcatct tgccaaaaac ggtaaaaact tatgcgtagc
    10621 agcgatattt taattgtaga cgacgaaatc ggcatccgcg acctgctgtc ggaaatcctg
    10681 caggacgaag gttattcggt cgcattggcg gaaaacgccg aagaggcgcg caagctgcgc
    10741 catcaggtac gcccggcgat ggtgttgctg gatatttgga tgcccgattg cgacggcatc
    10801 acccttttga aggagtgggc gaaaaacggg cagctcaata tgccggtggt gatgatgagc
    10861 gggcatgcca gcatcgatac cgccgtagaa gcgaccaaaa tcggcgcgct cgactttttg
    10921 gaaaaaccga tttccctgca aaagctgctg tctgccgtcg aaaacgcgtt gaaacacggt
    10981 gcggcgaaaa tcgaaacggg accggtgttc gacaagctgg gcaacagtgc ggcgattcag
    11041 gaaatgaacc gtgaggtagg ggctgcggtg aaacgtgcct ctcccgtact tttgacgggc
    11101 gaggcgggtt cgccgtttga aacggtggca cgctatttcc ataaaaacgg tacgccgtgg
    11161 gtcagcccgg caagggtcga atatctgatc gatatgccga tggaactgtt gcagaaggcg
    11221 gagggcggcg ttttgtatgt cggcgacatc gcccagtaca gccgcaacat ccaagccggt
    11281 attgccttta ttgtcggcaa ggcggaacac cgccgcgtca gggtggtcgc atcgggcagc
    11341 agggcggcag gttcagacgg catagcctgc gaggaaaagc tggcggaact gctgtcggag
    11401 tcggtcgtcc gcattccgcc gctgcgtatg cagcatgaag atattccctt cctgatacag
    11461 gggattgcct gcaatgtggc ggaaagtcaa aagattgcgc ccgccgcatt cagtgaagag
    11521 gcacttgccg cattgacccg ttacgactgg ccgggaaatt tcgaccaact gcaaagcgtc
    11581 gttgcaacgc tgttgttgga ggcggacgga caggaaatcg gcgcggcggc ggtttcttcc
    11641 cttttggggc agaatgtgcc tgccgaggga gcggaagata tggtgggcgg gtttaatttc
    11701 aacctgcccc tgcgcgaatt gagggaggag gtggagcggc gttatttcga gtaccacatc
    11761 gcccaagaag gtcagaatat gagcaaagtg gcgcagaagg tcggtttgga acgcacgcac
    11821 ctttaccgca aactcaaaca gctcggcatc ggcgtttcgc gccgggcggg ggaaaaaacc
    11881 gaagaatagg cccggacggt cggttttccg gctgcgggct tttgttttca gacggcattt
    11941 ggtgcaaatg ccgtctgaaa tcgtaagggg acggatttta tgacagagga cgaacgtttc
    12001 gcgtggttgc aactggcttt tacgccctat atcggtgcgg aaagtttctt gctgctgctg
    12061 caccatttcg gcagtgcgca aaatgccctg tccgcaccgg cggaacaggt ggcggcactg
    12121 atacggcaca aacaggcgct tgaggcttgg cgcaatgcgg aaaaacgcgc cttggcgcgg
    12181 caggcggcag aagcggcgtt ggaatgggaa atgcgggacg gatgccgtct gatgctgctt
    12241 caggatgaag atttccccga aatgctggcg caggggctga ccgcgccgcc agttttgttt
    12301 ttgcgcggca acgtgcggct gctgcacaag ccttccgctg ccatcgtcgg cagccgctat
    12361 gccacgccgc aggcgatgcg gattgccaaa gatttcggca gggcgttggg tgggaaaggc
    12421 attcccgtcg tgtcgggtat ggcttcgggc atcgacaccg ccgcccatca gggcgcgttg
    12481 caggcagaag gcggcaccat cgccgtgtgg gggacgggca tagaccgcat ttatccgcca
    12541 gccaacaaaa accttgctta tgaaatcgcc gaaaaaggac tgattgtcag cgagttcccc
    12601 atcggcacgc ggccgtatgc cggcaatttt ccgcgccgca accgtctgat tgctgccctg
    12661 tcgcaggtta cgctggtggt tgaagccgcg ttggaatccg gttcgctgat taccgccggc
    12721 ctggcggcag aaatggggcg cgaagtgatg gcggtacccg gctcgataga caatccgcac
    12781 agcaaaggct gccacaaact gattaaagac ggcgcgaaac tgacggaatg tttggacgac
    12841 atcctgaacg aatgcccggg gctattgcaa aatacgggtg cttcatcata ttctataaat
    12901 aaggatacgc ccgacacggg ccgccgcgcc gttcagacgg catacgcccc gccgcctgcc
    12961 gcaaaaatgc cgtctgaagg ggcggcaggc ggcacagccg tcggcggcat actggataaa
    13021 atgggtttcg accccatcca tcccgacgtg cttgccggac agttggctat gcctgccgca
    13081 gatttgtatg ccgcactgtt ggaattggaa ttggacggca gcgttgccgc aatgcccggc
    13141 ggcagatacc agcgtatccg aacttgaacg cactttatat taaggaacac gaatgaccga
    13201 agtcatcgcc tacctcatcg aacatttcca agatttcgat acctgtccgc cgcccgaaga
    13261 cttgggtatg ctgcttgaag aagcgggttt cgatacgatg gaaatcggca acaccctgat
    13321 gatgatggaa gtattgctca acagctccga attttccgcc gaacctgccg acagcggcgc
    13381 attgcgcgtg tacagcaaag aagaaaccga caacctgccg caggaagtga tggggctgat
    13441 gcagtatctg attgaagaaa aagccgtcag ctgcgaacag cgggaaatca tcatccacgc
    13501 gctcatgcac attccgggcg acgaaattac cgtagatacc gccaaagtgc tgaccctgct
    13561 gcttttatgg gcaaacaaga gcgagctgcc cgtgttggtc ggcgacgagc tgatgagcgc
    13621 gcttttactc gacaacaaac ccacgatgaa ctgaagcggc ttcagacggc ccgcccgagg
    13681 ccgtctgaaa cgccggcatc aaaaccacca tccagagaac gacaaatggc gaaaaaccta
    13741 ttaatcgtcg aatccccgtc caaagccaaa accctgaaaa aatatttggg cggcgatttt
    13801 gaaatccttg catcctacgg acacgtccgc gacctcgtcc ccaaaagcgg cgcggtcgat
    13861 cccgacaacg gctttgcgat gaaataccaa ctcatcagcc gcaacggcaa acacgtcgat
    13921 gccatcgtcg ccggtgccaa agaagctgaa aacatctacc tcgccaccga cccggatagg
    13981 gaaggcgaag ccatttcctg gcatcttttg gaaatcctca aatccaaacg cggcttgaaa
    14041 aacatcaagc cgcagcgtgt cgtgttccac gaaatcacca aaaacgccgt gctcgatgcc
    14101 gttgcccatc cgcgcgaaat cgaaatggac ttggtcgatg cgcaacaagc ccgccgtgct
    14161 ttggactatt tggtcggctt taacctttcg ccattgttat ggaaaaaaat ccgccgaggt
    14221 ttgagcgcgg ggcgtgtgca aagccctgct ttgcgcctga tttgtgagcg cgaaaacgag
    14281 atccgcgcgt ttgaagcgca ggaatattgg acggtacatc ttgacagtca caaaggacgc
    14341 agcaagttca ccgccaaact cgcccaatac aacggcgcga aactcgaaca attcgacctg
    14401 ccgaacgaag ccgcgcaagc cgacgtgttg aaagaactcg aaggcaaaga ggccgtcgtt
    14461 accgccattg aaaagaaaaa gcgcagccgc aatcctgccg cgccgttcac cacttctacc
    14521 atgcagcagg atgccgtgcg caaactcggc ttcaccaccg accgcaccat gcgtaccgcc
    14581 caacagcttt atgaaggtat agacgtaggg cagggcgcga tcggtctgat tacctatatg
    14641 cgtactgaca gcgtgaattt ggcggatgaa gcgttaaccg aaatccgcca ttacatcgaa
    14701 aacaaaatcg gcaaagaata tctgccgagt gccgccaaac aatacaaaac caaatccaaa
    14761 aacgcccaag aagcgcacga agccatccgt ccgacttccg tgtaccgcac gcccgaaagc
    14821 gtcaaaccct tcctgagcgc agaccagttc aaactctatc aaatgatttg gcagcgtacc
    14881 gtcgcctgtc agatgacgcc tgccaaattc gaccaaacca ccgtcgatat taccgtcggc
    14941 aaaggcgtat tccgcgtaac cggacaagtg caaaccttcg caggcttctt gagcgtttat
    15001 gaagaaagca gcgacgatga agaaggcgaa gacagcaaaa aactgcccga aatgagcgaa
    15061 ggcgataaat tgcccgtgga caaactctac ggcgaacaac actttaccac tccgccgcca
    15121 cgctacaacg aagccacgct ggttaaagcc ctcgaagaat acggcatcgg ccgcccctcg
    15181 acctacgcca gcatcatctc cacgctcaaa gaccgcgaat acgttaccct tgagcaaaaa
    15241 cgcttcatgc ccaccgacac aggcgacatc gtcaacaaat tcctgaccga acacttcgcc
    15301 caatacgtcg attaccactt taccgccaaa ctcgaagacc agcttgacga aatcgccggc
    15361 ggcaaacgcc aatggattcc cttgatggac aaattctgga aaccgttcat caaacaagtg
    15421 gaagaaaaag aaggcatcga acgcgccaaa tttaccacgc aggaacttga tgaaacctgc
    15481 ccgaaatgcg gcgaacacaa actgcaaatc aagttcggca aaatgggccg tttcgttgcg
    15541 tgtgccggct atcccgagtg cagctacacg cgcaatgtca atgaaaccgc cgaagaagcc
    15601 gcagagcgca tcgccaaagc cgaagccgaa caggcagaac tcgacggacg cgaatgtcct
    15661 aaatgtggtg gacggctggt gtacaaatac agccgtaccg gcagtaaatt catcggttgc
    15721 gccaactacc ccaaatgcaa acacgtcgag ccgctggaaa aaccgaaaga taccggcgtc
    15781 cagtgtccgc aatgtaaaaa aggcaacctc gtcgagcgta aatcccgcta cggcaaactg
    15841 ttttacagtt gcagcaccta tcccgactgt aactacgcca catggaaccc acccgttgca
    15901 gaaagctgtc caaaatgcgc atggccggtt ttgaccatca aaaccaccaa acgctggggt
    15961 gtagaaaaag tctgcccaca aaaagaatgc ggctggaaag aacagattga accgcccgcg
    16021 ccgaaggagt aagattaggt tggtttgaaa gagaaaaggt cgtctgaaaa attttcagac
    16081 gacctttgct tttctgtgat tggtttattt gaatccgcgt gttgttttaa agtccgataa
    16141 aatccggttc atttcaggcg caaacaaggc gatgtaatcg taagatagac cgcgactggc
    16201 actgggatgg ggaaagcaga cgacttcgca atcttcaaac gattggaatt tgacattgaa
    16261 acgtgtaccg tcaaattctt tttgcaccgt ctccagcggt ttggtctgct taccgaccaa
    16321 ctgctcgaag cgtggcagta cattttggtt gttcagaaaa tccgccaacc tgctgcccat
    16381 gaagaggatg actttcggac gcagtttttc gatgtggtag agaaaattat cgatgtgctc
    16441 gggttgtgtg aacttgtctg gattgtcgat agtgttgccc tgtgtagcag cccagttggt
    16501 ttgaaccagg gatttttcaa atgcaccgcc caatccattt tcgtctaagg ggtgtcccca
    16561 catttcaaac caatttttta tcgtattatc gtaacgccac ttttttgcct gctctccgaa
    16621 atagagggat ttgtttgcaa atgtatggtc gattttgttt tcagggagtt tgtattcacc
    16681 tgctacataa gcagcctcat cggctttact ccaaccccat tcatagccac aaatcattaa
    16741 gccatgtttg tcgttgtagc ctttgaacag gctgttgctc aaattcaaat ccttcatcat
    16801 gaactcttcc ttttaaaatt taagagcgat tgacttcaat gtttttagat ggggtggaaa
    16861 aatccttgtg taggcaacat aaattcaata aatttcttga taattcgaaa cctactaata
    16921 gcgcacctat aaaagctttt tcattacgtt cagcatgacg gtcacgtcgt tcatattttt
    16981 tacgcttgct gttccctgtt attacagcta agccaagtga tatggcgaga attgcccaaa
    17041 caatagtact taaataacaa ttttccccat actatcaata aggaaagaaa aaacctttta
    17101 gtattaggtc gatagattat aatccatgcc catgaaaatg caataagagt tatacataag
    17161 atacaagctg acaggatttc tttattttta attaaataac ttagagcgat gaggatgaca
    17221 ggtgtcaaga aaataatagt tacatcccga tagctataaa agaaaactgc cctattttga
    17281 atgtggagat gtgcacagaa tccaatatag ctaaggataa tagttaatat aataaaaaaa
    17341 gaccaccaag ggtgaagaga taggaattcc atgttttccc tttttttgta aaaaggaaaa
    17401 aatcttatct aatagttaat tgcctaacca gccagaagaa gtttaaaatc tatcccaata
    17461 attcaaccat ctatacagaa agttcagctt atggaaaccc acgaaaaaat ccgcctgatg
    17521 cgcgaattga ataaatggtc ccaggaggat atggcggaaa agctggcgat gtcggcagga
    17581 gggtatgcca aaatcgaacg gggcgaaacg cagttgaata tcccgcgttt ggagcagttg
    17641 gctcagattt tcaaaatcga tatgtgggac ttgctcaaat cgggcggtgg tgggatggtg
    17701 tttcagatta atgaaggtga tagtggtggc gatattgcgt tgtatgcgtc gggtgatgtt
    17761 tcgatgaaaa tagaattttt aaaaatggag ttgaaacact gcaaagaaat gttggaacaa
    17821 aaagacaaag aaatcgagct gctccgcaag ctgaccgaaa ccgtttaaac agatatgccg
    17881 tctgaaaaaa gttttcagac ggcatattct ttgacaggtc ttgtataata ccgtttgaac
    17941 ttacaggttt ttgattatgg cggcaggcaa acataccaaa cacagcaacc gggtacgcat
    18001 tatcggcggg caatgccggg gcaggaaatt gagtttcaca tccgccgacg gactgcgtcc
    18061 gacacccgac agcgtgcgtg aaaagctgtt taactggctg ggacaggatt tgacgggtaa
    18121 aacggttttg gatctcttcg gaggcagcgg cgcactcggt atagaagccg cttcgcgcaa
    18181 cgccaaacgc gtgctgattt cggataacaa ccgccaaacc gtgcagacct tgcagaaaaa
    18241 cagtcgcgaa ctgggtttgg ggcaggtgca aatcgtctgt tcagacggca tcgcatattt
    18301 gaagaccgta tccgaacagt ttgatgttgt ctttctcgac ccgccgtttg catggcagga
    18361 ctggcaaatc ctgtttgatg ccttgaagcc gtgcctgaac ccccgagcat tcgtctatct
    18421 cgaggcgggt acgctgccgg atattcccga ttggctgacg gaatatagag aagggaaatc
    18481 ggggcagagt acattcgaat taagggtttt ccaagtggct gaataatatg cgctttgata
    18541 atcatttccg agttgtaaac attcgtttgc aaccgtccgg ttcaaaaaaa ccttgtgcta
    18601 taatccgcgc ccgcccggtt ttgataattt agtggaaaag gaaaagaaat gtcgcttttt
    18661 attaccgacg agtgcatcaa ctgcgacgta tgcgaacccg aatgccccaa tgatgccatt
    18721 tcccaaggcg aggaaattta cgaaatcaac cccaacctct gcacgcagtg cgtcggacac
    18781 tacgatgagc cgcagtgcca gcaggtttgc ccggtggact gcatcctgat tgacgaagaa
    18841 catcccgaaa cccatgacga gttgatggcg aaatacgaaa agattatcca gtttaaataa
    18901 attcttttta aaacatcaaa ttatgtctgt tttgaaataa aatcaaaaaa aaacttgacg
    18961 gaaaagcaag ccgctaataa actaacgttc tcttttggag ggattcccga gcggtcaaag
    19021 ggggcagact gtaaatctgt tgcgaaagct tcgaaggttc gaatccttct ccctccacca
    19081 aaattcttac ttggggcagt agcgagtaat gcgggtgtag ctcaatggta gagcagaagc
    19141 cttccaagct tacggtgagg gttcgattcc cttcacccgc tccaaacaat taggcccatg
    19201 tagctcaggg gtagagcact cccttggtaa gggagaggtc ggcagttcaa atctgcccat
    19261 gggcaccatc tctcgattat tcatttcttt aaggcttaga tatataggat attg
//
LOCUS       DAWKST010000020        18989 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.020, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000020 DAWKST010000000
VERSION     DAWKST010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18989
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.020"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(375..650)
                     /locus_tag="WJL87_000489"
     CDS             complement(375..650)
                     /locus_tag="WJL87_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ7798960.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(647..1930)
                     /locus_tag="WJL87_000490"
     CDS             complement(647..1930)
                     /locus_tag="WJL87_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7798961.1"
                     /translation="MTVSKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRVAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAVTVLGFVWLAGFGPFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFALALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(2084..3502)
                     /gene="glnA"
                     /locus_tag="WJL87_000491"
     CDS             complement(2084..3502)
                     /gene="glnA"
                     /locus_tag="WJL87_000491"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ7798962.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDVTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDCGQDLRSAMVNILEGLGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            complement(3776..4153)
                     /locus_tag="WJL87_000492"
     CDS             complement(3776..4153)
                     /locus_tag="WJL87_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798963.1"
                     /translation="MKKMLCLAVFALGLATPAVAADWVLVDSNTATFGDADSRTDNSA
                     WFETRFAKPQKYGNGKFFNTAKTLEEMDCSGKRFRILTATWYSKSGNSIASYTPSYAE
                     WNYVIPGTLGEAQYKFVCNGYPR"
     gene            4325..5134
                     /gene="aroE"
                     /locus_tag="WJL87_000493"
     CDS             4325..5134
                     /gene="aroE"
                     /locus_tag="WJL87_000493"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ7798964.1"
                     /translation="MTALPRYSVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIPLKNGKLRGD
                     NTDGIGLTNDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELAQLFGIEAVPMADVNGGFDIIINGTSGGLNGQIPDIPPDIFQNCALAYDMVYGCA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFTPDIRPVIEYMKAM"
     gene            5136..5837
                     /gene="mtgA"
                     /locus_tag="WJL87_000494"
     CDS             5136..5837
                     /gene="mtgA"
                     /locus_tag="WJL87_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ7798965.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWVPYNRISVNLKKALIASEDAHFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(6040..6115)
                     /locus_tag="WJL87_000495"
     tRNA            complement(6040..6115)
                     /locus_tag="WJL87_000495"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6080..6082),aa:Phe,seq:gaa)
     gene            complement(6306..7040)
                     /gene="lptB"
                     /locus_tag="WJL87_000496"
     CDS             complement(6306..7040)
                     /gene="lptB"
                     /locus_tag="WJL87_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ7798966.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVMLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEISTKDKSRIDGEVEKLLADLNIERLRNNPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGTV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(7114..7644)
                     /gene="lptA"
                     /locus_tag="WJL87_000497"
     CDS             complement(7114..7644)
                     /gene="lptA"
                     /locus_tag="WJL87_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ7798967.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(7625..8206)
                     /gene="lptC"
                     /locus_tag="WJL87_000498"
     CDS             complement(7625..8206)
                     /gene="lptC"
                     /locus_tag="WJL87_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ7798968.1"
                     /translation="MKIRWRYGIAFPLILAVALGGLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(8203..8739)
                     /locus_tag="WJL87_000499"
     CDS             complement(8203..8739)
                     /locus_tag="WJL87_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ7798969.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
     gene            complement(8804..9778)
                     /locus_tag="WJL87_000500"
     CDS             complement(8804..9778)
                     /locus_tag="WJL87_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ7798970.1"
                     /translation="MAENGKYLDWAREVLHTEEEGLREIATELDENFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAARIV"
     gene            9875..10930
                     /gene="tal"
                     /locus_tag="WJL87_000501"
     CDS             9875..10930
                     /gene="tal"
                     /locus_tag="WJL87_000501"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ7798971.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKQQDLTPKQRYETMAVADVQAACDVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRVQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDGALDTTLPDHLK
                     GKIAIALAKAAYQDWAQYFGSPEFAALETQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGADEAQAQLAETAALGIDVETLAARLQED
                     GLKQFEEAFEKLLAPLV"
     gene            11084..11374
                     /locus_tag="WJL87_000502"
     CDS             11084..11374
                     /locus_tag="WJL87_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798972.1"
                     /translation="MAKALEIISPNEIYSDLIFKDPVPPHTIYTELMKLVSRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALINSLK"
     gene            11391..12278
                     /gene="gluQRS"
                     /locus_tag="WJL87_000503"
     CDS             11391..12278
                     /gene="gluQRS"
                     /locus_tag="WJL87_000503"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ7798973.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAAASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLQTAGLVYPCHCSRK
                     DWQAAAVHGADGFVYNGRCRNPQQRPALQGKQPAWRIRVPDRDIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMGKVPKHAITTP"
     gene            complement(12348..13358)
                     /gene="dusA"
                     /locus_tag="WJL87_000504"
     CDS             complement(12348..13358)
                     /gene="dusA"
                     /locus_tag="WJL87_000504"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ7798974.1"
                     /translation="MNDNTHTLPPRHLSVAPMLDWTDRHYRYMARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVGIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQCLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGSLILEAWKEVERANT
                     RE"
     gene            complement(13389..13880)
                     /locus_tag="WJL87_000505"
     CDS             complement(13389..13880)
                     /locus_tag="WJL87_000505"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ7798975.1"
                     /translation="MRAVIQKTVGAKVDVVSENGTETCGKIDGGFVVLLGVTHSDTEK
                     DAHYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(13954..14712)
                     /locus_tag="WJL87_000506"
     CDS             complement(13954..14712)
                     /locus_tag="WJL87_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7798976.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDAILEENGVKP"
     gene            complement(14783..15649)
                     /locus_tag="WJL87_000507"
     CDS             complement(14783..15649)
                     /locus_tag="WJL87_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7798977.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLRAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(15698..15976)
                     /locus_tag="WJL87_000508"
     CDS             complement(15698..15976)
                     /locus_tag="WJL87_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ7798978.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(15976..16266)
                     /locus_tag="WJL87_000509"
     CDS             complement(15976..16266)
                     /locus_tag="WJL87_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ7798979.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(16269..16799)
                     /locus_tag="WJL87_000510"
     CDS             complement(16269..16799)
                     /locus_tag="WJL87_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ7798980.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVIFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            17107..>18989
                     /locus_tag="WJL87_000511"
     CDS             17107..>18989
                     /locus_tag="WJL87_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HEZ7798981.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     DGLFGGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEI
                     TFAEVETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLA
                     AAQNGILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESV
                     AQTAENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTV
                     AEVQTPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENVTETV
                     AETP"
BASE COUNT         4502 a         5418 c         4771 g         4298 t
ORIGIN      
        1 ctctttaagt tgacatattg tgcgatgtcg agggctgtac cgtattttcc catcgtattt
       61 tcccatcgta ttttcccatc gtattttccc atcgtatttt cccgttcttt atatgagcgg
      121 caggcatcct cataaaagaa gggataccga taaggaaaaa taccccagca taaaaaatat
      181 actgattaat caatacaaat ccaacaaaca gttcatcgta ttcttttctg ttggcgtata
      241 aaaatactga tatattcctt cactttcata gggcgacgct ttatgtaccg tcctgtgtgt
      301 tgaaacgtcg gttttcctta ccgcctgccc cgcttttccc tgattgctgc caactgccgt
      361 ccgaacttga accgtcaggt ttcagacggc attttttctg cctgctccca caattgccgt
      421 ttttgtgcgg cggtcagttt tttgacggcg atttcctgcc tgagtgccgt gcctttatcc
      481 atcccgcctg caacgatacg catcgccacc ggtttgaata cgcgggtata tttcgcgcct
      541 ttaccggttg tgtgggcggc aagccgctgt tgcggattcg ggctgatgcc gcaatagaac
      601 gcgctgtttt cacacaatat caggtaaacg ctccagtttg acgcgttcat ctgcctgcat
      661 cctgagtttt ctccccgttc caaggcgcaa ctttcagcag cagcagcata cccggtaggg
      721 cgagtgcgaa acacagttgg aaaaacggta cataaccgag ccattcaatc agataaccgg
      781 caaaggaatt gatgaccgtg cgcgggacgg cggacaggct ggtaaacagc gcaagctgcg
      841 ttgcggtaaa tgcgggattg gtttcacgcg ccatatacga tacgaacgcc gccgtcccca
      901 accccacgcc gaccgcttcc gcgccgataa ctgccgccag catcagcctc tcgcctgtgc
      961 cgaccgtgtc gaaaggtccg aaccctgcca gccatacaaa ccccaaaacg gttacagcct
     1021 gcaccgcgcc gaatagccac aaggctttgt ttacgccgat tttcagcatc cacacgccgc
     1081 ccaagatacc tgccgccact gccggccaca gtcctgcatt tttcgcaatc aaaccgatgt
     1141 ctgtcttgct gaaacccata tccagataaa acggcgttgc caacgcggtt gccatactgt
     1201 cgccgagttt gtaaaggaag ataaacagca gcacgcacac cgccgaagcg atgcccttgc
     1261 gcgtaaaaaa ttctttaaac ggctctacca cggtctgctt caacgtttta ggaacggcag
     1321 gcggcaacac gggttcgcgc gcaagaaaca gcgtcatcag aaggccgggc agcataaata
     1381 atgaagtgat aacaaatact tctgaccacg gcatcctgtc tgccaacacc aaactcaatg
     1441 aaccgggaat cagggcggca acccgatagg cgttcacatg aaccgagttg cccaaaccca
     1501 attcttcgtc cgacaaaatc tcgcgcctga acgcatccaa tacaatgtcc tgactggcgg
     1561 aaaaaaaagc gacaagcacc gacaagccgg caatcagcgg cagatgatta cgggggttta
     1621 aaaaggcata ggccgccaaa gccgccagca accctgcctg cgtcagcagc atccacccgc
     1681 gccgccgtcc caaaacgggc agcctgaccg cgtccatcag cggcgaccac aaaaatttcc
     1741 aagtaaacgg cagaccgatt aacgccatca gcccgatgct cttcaaatcc acctgctcgc
     1801 tgcgcaacca cgccggaatc aggttaatca gaaagtacag cggcagcccc gaggtaaacc
     1861 ccgtaaaaat acaaacaagc atatttcggg aaaagatttg cccgataaaa cctgatttcg
     1921 atacagtcat ggttcaaaca gccaagaaat aaaagcctat tgtagcaaat atcgcccaac
     1981 cgctgaaaaa caccgcccct gctccgccgt tttgccgttc aaatgcaaaa tgccgtctga
     2041 aactgtgttc agacggcatt gcttttctga aaacccgacg tgcttacagg ctgtaataca
     2101 tttcaaattc cagcggatgc ggtgccatgc ggatgcggcg gacatcttcc tctttaaagg
     2161 cgatatagct gtcgatccag tctttgctga acacgccgcc gcgcaggagg aattcgtggt
     2221 cggctttgag ggcggcgagg gcttcttcca aagaggcgca aacggtcggg acgagcgcgt
     2281 cttcttccgg cggcaggtcg tacaggtttt tatcggcagg atcgcccgga tggattttgt
     2341 tttgaatacc gtccaaaccc gccatcaaca gggcggcaaa tgccaaatac gggttggcgg
     2401 tcggatcggg gaaacgcgct tcgatgcggc gcgccttgct gctgttcacg gacggaatgc
     2461 ggatggaagc ggaacggttt ttggcggaat atgccagttt ggtcggtgct tcaaagtgcg
     2521 gcacgaggcg tttgtaggag ttggtggacg gattggtaat tgcgttcagg gctttggcgt
     2581 gtttgatgat gccgccgatg tagtagagcg cggtgtcgct caagccggca tagccgtcgc
     2641 ctgcgaacag gttttgaccg tctttccaaa tggattggtg aacgtgcata ccgctgccgt
     2701 tgtcgcccat aatcggtttg ggcataaagg tggctgtttt gccgaagttg tgggcaacgt
     2761 tttgaatcac atatttcatg tcttgggttt ggtcggcgcg tttgaccaag gtggcgaaac
     2821 gcgtgccgat ttccatttgg ctgccggtac cgacttcact gtggtggact tcgacttcga
     2881 tgccgagtcc ttccaaaatg tttaccatcg cggaacgcaa atcctgaccg cagtcaatcg
     2941 gcgcgacggg cgcgtagccg cctttgacgg cgggacggtg gccggtgttt tgaccgtcca
     3001 tatgcaggcc gcttgcccac gcgccgcttt cggacgtgat ttcgtaacgg gttttgtgca
     3061 tgtcggtttc aaattctacg ccgtcgaaga cgaagaattc gggttcgggg ccgaagtatg
     3121 ccgtgtcgcc gatgccggaa gatttcaaat aggcttcggc gcggcgggcg atggagcgcg
     3181 ggtcgcggtc gtaaccctga ccgtcggcag ggtcgatgac gtcgcaggta atgacgacgg
     3241 taacatcatc ataaaaagga tcgacgaagg ctgtagacgc atcgggacgc aactgcatat
     3301 cggaagcctg aatgcctttc cagccgccga tagacgaacc gtcaaacgcc tgaccgtttt
     3361 cgaaccactc ttcggggtct tccaacacga tgcgcgcagg cacggtaaag tggtgctgct
     3421 tgcctttggt atcggtaaag cgcaaatcga caaagcgggc ttcgctttct tcaatcaatt
     3481 ttacggcgtt tttaatagac atttttcagc tcctggaaaa gggaaatagc aaaatatgcg
     3541 gaacgcggca gattagctca aagccagcat gattctacca cgtccatgcc gtctgaaact
     3601 ggccctgcgt caaattccaa gctgtaaaat atagtttcag acggcaatat tgtcaacaat
     3661 atgcttcggt ttgcccgttc ccaagcagaa tgcccgccct tttatccgaa tgccgacaaa
     3721 acagatgccg tctgaaagca gttcagacgg catctgttgt ttggtttgaa aacggtcagc
     3781 gaggatagcc gttgcagaca aatttgtact gagcctctcc aagcgtaccg ggaataacat
     3841 aattccattc ggcataggat ggtgtataag aagcgataga gtttcccgat ttggaatacc
     3901 acgttgccgt caatatccta aaacgtttac cactgcaatc catttcttcc aacgttttag
     3961 ctgtattaaa aaattttcca ttgccatact tttgcggctt ggcgaacctt gtttcaaacc
     4021 atgcactgtt gtccgtacgg ctgtctgcat caccaaacgt ggcagtatta ctatctacca
     4081 atacccaatc cgccgccacc gccggtgttg ccaaccccaa tgcaaatact gccaagcaca
     4141 acatcttttt catgtccgac cctctcgtcc ttgtcaacaa aataaagtta aattgtaaaa
     4201 aaaaaacgac ttttttcaag agaaaaatat tccgtcatgc tatctgacaa ccgtttcaga
     4261 cggcatcgtt tccatctgta cgccgccgtt ataaaatagc cttcccgtct tttggaacac
     4321 gaccatgacc gccctccccc gttactccgt ttttggcaac cccgttgcac acagtaaatc
     4381 gccgcaaatc catcagcaat ttgcccttca ggaaggcgtt gacattgaat acgaacgcat
     4441 ttgcgccgac atcggcggtt ttgcacaggc ggtttcgaca ttttttgaaa caggcggttg
     4501 cggggcaaac gttaccgtac cgttcaaaca ggaagcgttt catctggcgg acgagcattc
     4561 cgaacgcgca ttggcggcag gtgcggtcaa tacgctgatt ccgttgaaaa acggcaagct
     4621 gcgtggcgac aacaccgacg gtatcggttt gaccaacgac atcacgcagg tcaaaaatat
     4681 tgccatcgag ggcaaaacca ttttgctttt gggcgcaggc ggcgcggtgc gcggcgtgat
     4741 tccggttttg aaagaacacc gccctgcccg tatcgtcatt gccaaccgta cccgcgccaa
     4801 agccgaggaa ttggcgcagc ttttcggcat tgaagccgtc ccgatggcgg atgtgaacgg
     4861 cggttttgat atcatcatca acggcacgtc cggcggtcta aacggtcaga ttcccgatat
     4921 tccgcccgat atttttcaaa actgcgcgct tgcctacgat atggtgtacg gctgcgcggc
     4981 aaaaccgttt ttagattttg cacgacaatc gggtgcgaaa aaaactgccg acggactggg
     5041 tatgctagtc ggtcaagcgg cggcttccta cgccctctgg cgcggattta cgcccgatat
     5101 ccgccccgtt atcgaataca tgaaagccat gtaatatgtt ccgcatcgtc aaatggctga
     5161 ttgccctgcc cgtcggcatc tttatctttt tcaatgccta tgtgtacggc aacatcatta
     5221 cctaccgcgc cgtcgcgccc catcggactg cctttatgtc gatgcggatg aagcagtttg
     5281 aacaggaagg tcgcgatgtc gcactggatt accgctgggt gccctacaac cgcatctcgg
     5341 tcaacctgaa aaaagccctg attgcttccg aagatgccca ttttgccggg cacggcggct
     5401 tcgattgggg cggcattcaa aacgccatca ggcgcaaccg gaacagcggc aaagtgaagg
     5461 cgggcggctc gaccatcagc cagcagcttg ccaaaaacct gtttttaaac gaaagccgca
     5521 gctatatccg caaaggcgaa gaagcggcga ttaccgcgat gatggaagcc gttaccgaca
     5581 aagacaggat ttttgaactg tatttaaact caatcgaatg gcactacggc gttttcggcg
     5641 cggaagccgc gtcccggtat ttttatcaaa tacccgccgc caagctgacc aaacagcagg
     5701 cggcgaaact gactgcccgc gtccccgccc cgctctacta cgccgaccat ccgaaaagca
     5761 aacggctccg caacaaaacc aatatcgtgc tcagacgcat gggttcggca gagttgcctg
     5821 aaagcgacac ggactgagtg ccgccgcaat ataaaatgcc gtctgaaata tcattcagac
     5881 ggcatatgct ttctgccttt acccttttct ctaaaacgca acggggcggt cattatcgct
     5941 tttgtccttc tacataacgg tcagacacaa aacgtgtccg acacggacaa cttcccaaaa
     6001 acggcaggca aaaaaaataa ccgcttataa gcggtttttt ggtggccaga ggcggaatcg
     6061 aaccgccgac acacggattt tcaatccgtt gctctaccaa ctgagctatc tggcctttgt
     6121 ctgtctcgtt aagagatgcg tattaaacca aattacggga cgacatgcaa gcatatcggg
     6181 caatattctt tgaaaacagg tttaagtcaa tgttttaaat ttgaattaat ttatgtaaaa
     6241 acaacataaa tccgtatttt tgccgcctgc ccgacgatat taggtcgccc gtctgaaaaa
     6301 tattttcaat atttgaagtt cttacccaga taaacagaac gaacctgttc gtttccgacc
     6361 aaatcatcag gttttcccga tgccaacacc gtgccgtctg aaataatgta ggcccgatcg
     6421 cagatgctga gggtttcgcg tacgttgtgg tcggtaatta atacgccgat accgcgcgac
     6481 ttgagaaatc cgatgatttt ttggatgtcg atgacggcga tgggatcgac gccggcaaaa
     6541 ggctcgtcca acaaaataaa atgcggtttc atggcaagta cgcgggcgat ttcgacgcgt
     6601 cggcgttcgc cgcccgacaa agatggggca ggattattgc gcaaacgctc gatattcagg
     6661 tcggcaagca gtttttcaac ttcgccgtcg atgcggcttt tgtctttggt actgatttct
     6721 aaaatggcgc ggatgttttg ttcgacggtc attttacgga aaatagaggc ttcctgcggt
     6781 aggtagccga cacccaggcg ggcgcgttcg tgtatgggca ggtgacgcaa ttcctgcccg
     6841 tccagcatca cgctgcccgc atcggcggca atgagtccaa caatcatgta gaagctggtg
     6901 gttttacccg caccgttggg cccgagcagg ccgacgactt ccccgctttc gatttcaagg
     6961 gagaagcttt taacgacttg gcgttttttg aaactttttt gcaggttttg aacaacaagg
     7021 cggctgacgt ttgcgctcat aaatatctct tcaatatctt cggtcggcaa atgccgtccg
     7081 aaccaggttt atgtttcaga cggcattttg ggattattcg gatttttgcg tactcgaagg
     7141 ctggataacg acgctgaccc tgccggattt ggaagcggat tttgcgccgg attttgtgct
     7201 gccgctgatg gtatagactt cggttttggt gttgtatgta atcaccgcac cttcggcgac
     7261 atcgccgccg cgctgtactt tggcattacc ggttaagact acggtgctgc ctgcagatga
     7321 ataagcaacg ttgtttgcct gtccgcgcac cgtgcctttg ccgccgtcca atgtctggct
     7381 gaagcggact ggcgaacctt ccgccctcac ggattcgccg cctttgccgc cgcgtgtaac
     7441 attgacgcgg gcggcggaaa tattgagcgt accctgtctg atgacgacgt ttccgctgaa
     7501 tgtggtgctt tggttggctt gatcgagcga accttggtcg gcctcaatct gaataggctg
     7561 cctgctgtcg ctttgaaggg caaaagcggg ggacgccgaa aaaaatgcta ttaaaacaaa
     7621 tagcttacat atcttttgta tcataaatcg tggctttcac tttagatgag aagttcaaca
     7681 tgcctgtttt gtggtcgtaa gtcatgccgc ccgcctgacc gtgcgatgca ccatattgga
     7741 aactgacagg cgtatcggtt tgggcatatt gagattcggt atcgacgtgc agcttttcgg
     7801 cttcaacttt acccgcctgc cgtttgccgt cagcggtttt ggtcagcaca acgttgtttt
     7861 taaaaagaac ctgtttgttt tcggtatggt aaacggcttc atcgctgccg acttcgtaca
     7921 acaacctgcc ttcttggaag aacacgagat gcggcgaatc aaaatggatg tcgctgcttt
     7981 cgggaaactg tttcgcgcct ttcgcgctca aatgttcttt taagtatccc tgttcgtcaa
     8041 accgcctgcc atccaagccg tccattgtgt attgcggttc gtcgggattg agcctgactt
     8101 cttcaatctc gacttcgctg atgcgtccca accatgccga cagaccgccc aaggcaaccg
     8161 ccaatatcaa tgggaacgca attccgtacc gccatcttat tttcatttga tgtactcgtt
     8221 caaagccgcg cccaaagtcc cttgcgcctg cataatcagg tcgcacactt cgcgcaccgc
     8281 gcccgcaccg ccggggcgtt tggcaatata ggcggcgtgt tgcagcgtaa accaatgcgc
     8341 gtcgggaacg gcaaccggca atccgcaacg caccatcacc ggcaaatcga ccacgtcgtc
     8401 gccgacaaag gcacactcgg cttcttccac gccgacctga gcgcgcagct cttcatacgc
     8461 ggcgcgtttg tccgaaatgc ctttgaagta gtaatttatg cccaactgtt tgacgcggat
     8521 gccgacggag ggcgcgtccc gacctgtgat aatcgcagtc tgcacgccgc ttgcctgaag
     8581 cattttcaga ccgtgtccgt ccagtgtgtg aaacgatttg atttcttcgc cgttatcgcg
     8641 gataaagatg cgcccgtcgg tcaaaacgcc gtccacatcc aggatcaaca gtttgatttt
     8701 ggcggcgcgc gcctgtaatt cgggagaaat tgcctgcatg ggatttcctt gttttcagac
     8761 ggcatatctt accacaaatg ccgtctgaaa cggctgcaac gccttataca atccgtgccg
     8821 ccagcaggtc gtgcatattc agcgcgccga tcagcacgcc atctgcatcg gtaaccagaa
     8881 gcccgttcac atggtttgcc tgcatgactt tcagggcttc ggtggcgaga cgttcggcgg
     8941 agatggtttt aggatgcgta tgcatgactt cgtctatcga aagaccggta aaattgtcgc
     9001 attcttgaaa caggcggcgc aaatcgccgt cggtgaatac gcctttcaga cggccttgcc
     9061 cgtccgttac cgccaacatg cccagccctt tttcactcat gctgacgatg gcttctttca
     9121 agggcgtgcc gagtcggacg gcaggcaggc cgccgccttt gtgcataatg tcggcaacgc
     9181 gcaaaagtag gcgtttgccg aggctgccgg caggatggct caaggcgaaa tcgtcgggcg
     9241 tgaacgcgcg tgcgcgcagc aggacgaccg ccaacgcatc gcccaaagcc atgacggcgg
     9301 tggtgctggt ggtcggggca agccccagcg ggcaggcttc tttggaaacc gacgccgtga
     9361 tgtggatgtc ggcatggcgc gccatggttg aatcggggcg ggcggtgatg cagacaagcg
     9421 tgatgtcttt gcgtttgagc gcggggatga tggcggcgat ttcgtcgctt tcgccggaat
     9481 tggaaatcgc gacgaccacg tcgttgtcca caatcatgcc caaatcgccg tgtgccgctt
     9541 ccgcagggtg gacaaaaaac gcgggcgtgc cggtcgaggc catcgttgcc gccattttgc
     9601 gcccgatatg tcccgacttg cccatgcccg tgataacgac cctgcccttg cagtgcaaca
     9661 acgcgtctgc cgcaaggacg aagttttcgt ccaattccgt tgcaatttcg cgcaagcctt
     9721 cctcttcggt gtgcaacact tcgcgcgccc agtcgagata ttttccgttt tctgccattg
     9781 ccatccgcct ttgctgtaat aaaactaccc aaaatttccg aatcggggta gcataagcct
     9841 ttgaaacata cacatccatc caaaggaatc agaaatgact attttatcgg acgttaaagc
     9901 attaggacaa caaatctggc tggacaacct ttcccgctcg ctcgtgcaaa gcggcgaatt
     9961 ggcgcaaatg ttgaaacaag gcgtgtgcgg cgtaacttcc aatcccgcca ttttccaaaa
    10021 agccttcgcc ggcgatgccc tgtacgccga tgaagtcgcc gcgctcaaac agcaagatct
    10081 gaccccgaaa caacgctacg aaaccatggc ggttgccgat gtgcaagccg cctgtgacgt
    10141 gtgccttgcc gaacacgaat ccaccggcgg caaaaccggt ttcgtcagcc tcgaagtttc
    10201 gcccgaattg tccaaagacg cgcaaggcac ggtagaagaa gcgcgccgcc tctacgccgc
    10261 catcgggtgc aaaaacgcga tgattaaagt gcctgccacc gacgcaggca tcgatgcgct
    10321 cgaaaccctc gtttcagacg gcatcagcgt aaacctgacc ctgctgttct cacgcgtcca
    10381 aaccctcaaa gcctacgccg cctacgcgcg cggcattgcc aaacgcttgg cagccggaca
    10441 aagcgttgcc catatccacg tcgtcgccag cttcttcatc tcccgtgtgg acggcgcgct
    10501 ggacacaaca ctgcccgacc acctcaaagg caaaatcgcc atcgcccttg ccaaagccgc
    10561 ctaccaagac tgggcacaat acttcggcag cccggaattt gccgcactgg aaacccaagg
    10621 cgcaaaccgc gtgcagcttt tatgggcatc taccggcgtg aaaaacccgg cctatcccga
    10681 cacactctac gttgacagcc tgatcggcgc gcacaccgtc aacaccgttc ccgatgccac
    10741 gctcaaagcc ttcatcgacc acggcacagc caaagctacg ctgaccgaag gcgcggacga
    10801 agcacaagct cagctcgccg aaaccgccgc gctcggcatc gatgtcgaaa ccttggcggc
    10861 acgtttgcag gaagacggtt tgaaacagtt tgaagaagcg tttgaaaaac tgctcgcacc
    10921 gttggtttaa acttttcatc cccgatgccg tctgaaaggc tttcagacgg catcgcccgt
    10981 ttgaccggac acagtgcggc atatcaagga ttcaccctaa aaatattaat gggcaagaag
    11041 ttaatgcacg acattaccca ccaacacaaa aggaaataga atcatggcaa aagcactcga
    11101 aatcatttca cccaatgaaa tttattcaga tctgattttt aaggatccgg tacctcccca
    11161 tacaatatat acagaattga tgaaactggt tagtagaaat gcaagtaatg agagaatcgc
    11221 agatagagcc ttcgattttt tctcgtccgg ccttatggat ggttcagcta ccgaagagca
    11281 atataatgcc ctatatgatt taaccttact tgaagaaccc gggatggaac tgaataaaga
    11341 tgagataatg gctttgatta atagtcttaa ataaaggggc cgtaccggat atgtacacag
    11401 gacgtttcgc ccccagcccg accgggctgc tccacatcgg ctcgctgctg accgccgccg
    11461 cttcctatgc cgatgcacgc tcaaacggcg gcaaatggct ggtccgcatg gaagacctcg
    11521 atccgccgcg cgaaatgccg ggggcggcaa gccatatcct gcacacgctt gaggcattcg
    11581 gattcgagtg ggacggagaa gtcgcctatc agagccgccg ttacgccctg tatgaagaaa
    11641 cactatgccg tctgcaaacc gccggactgg tctatccctg ccattgcagc cgcaaagact
    11701 ggcaggctgc ggcagtccac ggcgcagacg gattcgtcta taacggacgt tgccgcaatc
    11761 cgcagcaacg ccctgcactg caaggcaaac agcctgcgtg gcgcatccgc gtccccgacc
    11821 gcgacatcgg cttttcagac ggcatcgtcg gcggttacgc ccaaaacctc gcccgcgaca
    11881 tcggcgattt cgtcctgctt cgtgcagacg gttactgggc ataccagctc gccgtcgttg
    11941 ccgacgatgc cgaacagggc gttacccaca tcgtccgcgg acaagacctg ctcgtttcca
    12001 cgccgcgcca aatctatttg cagcagtgtt tgggcgttcc gacaccgcaa tatgcccacc
    12061 tgccgctgct gaccaacgca caagggcaga agtggtcgaa acagacgctc gcccccgcat
    12121 tggatttaaa ccgccgcgaa caactcctcc gccaagtgtt ccgttacctc aagctgcccg
    12181 aagcaccgga aaccgaccgc cctgccgaac tgctcgactg ggcggtggca cactgggata
    12241 tgggcaaagt gccgaaacac gccattacca ccccctaaac accgcccgac aaacgcacaa
    12301 atgccgtctg aagccttcag acggcatttc acacatacag ccccgcccta ttcgcgcgta
    12361 tttgcccgct caacctcttt ccacgcttcg agaatcaggc tgccgtcgtt gtctttcaag
    12421 agcgttgcgt cggaaagcat acgccgccaa gtccgcgcac ctttcaaacc gtgcatcagc
    12481 ccgaggctgt ggcggacgat gtggcgcaag attgtgccgc gtccggcttg gatctgggct
    12541 tggctgtatg tgtagaggca ctgcaccaaa tcggcgtatt cgatcgggct gcgagtatcg
    12601 ccgtaaaaca atctgtccca ttcgcgcatc accatcgggt tgtggtacgc ctcgcgcccg
    12661 accatcacgc cgtcaacgtg ttgcaggtgt ccggcgattg cttcgttggt ggtgatgccg
    12721 ccgttgatga tgatttccag cccgggaaac tcctgcttga ggcggtaaac gtaatcgtat
    12781 ttcaacgggg gaacgtcgcg gttttctttg ggggaaagac cgtccagcca agcgttgcgg
    12841 gcgtggacga tgaaggtttt gcaggcggtt ttgtcgcgca gcgtgccgac gaaatcggca
    12901 acggtttggt attcggtctg cctgtccaca ccgatacggt gtttgacggt aacgggaata
    12961 ccgaccgcgt cctgcatggc gttgaggcag tcggcaacca gcccgacttc gttcatcaga
    13021 cacgcgccga acgagccttt ctgcacgcgc ggactggggc agccgcagtt gaggttgacc
    13081 tcgttgtaac cgtatgcctc ggcggctttg gcggctttcg ccaaatcgga cggatcgctg
    13141 ccgcccagtt gcagggcgac gggctgctcg ccttcgttga acatcaaaaa gcggtctttg
    13201 tcgccgtaaa caatcgcgcc ggcgttgacc atttcgctgt acagccaagt attgcgggta
    13261 atctggcgcg ccatataacg gtagtgcctg tccgtccagt ccaacatcgg ggcgacggac
    13321 aggtggcgcg ggggaagggt gtgtgtattg tcgttcattg tcgatatttt atcattgcgg
    13381 attcaatttc aatccggaac aaccttcatt tttggggaaa tccgcgtcat gaaagagtcc
    13441 agcagtatgg ttaccggccc gtcgttgcag agcgacacct gcatatgcgt gcggaaacgc
    13501 cctgtttcga catgaatccc gtgtccgcgc aacagttccg ccgttcgcag gtaaagctgc
    13561 tgcgcctgtt ctgcaggtgc ggcttgggaa aacgaaggcc gccgcccgct tgccgcatcg
    13621 gcataaagtg taaactgcga caccagcagc accgcgccgc cgacatcttt caaagacagg
    13681 ttcaacttgc ccgcttcgtc ttcaaacacg cgcaaatggg cgattttgtc ggcgatatag
    13741 tgcgcatctt tttctgtgtc gctatgcgtt acgccgagca gcacgacgaa tccgccgtcg
    13801 attttgccac aggtttccgt gccgttttcg gacacgacat ccacctttgc acctaccgtt
    13861 ttctgtatga ccgcacgcat aagccgtcct ccttacctga acgtgcaatg ccgtctgaaa
    13921 aggcttcaga cggcatcggt attggaaatg ccattacggt ttgacaccgt tttcttccaa
    13981 aatggcatcg attttcaagc gggctttttc ctgtctcaaa ccttgttcca actggtttct
    14041 gaccaactcg aaaggctgcg cgtcggggtt tttcccgacc tcgctgagtt tgaacagata
    14101 atagcgttcg cccaatttga ccggatcgcg ggtaacgtcg ccccgattca tcgctgcaaa
    14161 ctgcgaagcc agcggctcgg gaagctgctg cgccataatg aaaccgtcaa aagcctgctc
    14221 gtcgttcgga taacgcttca tcagcccttc aaaagacagc cctttgagca ggagctgctg
    14281 cgcctgacgc gcctcctctt cggttgcgaa gctgacctgc tgcaatttga tcatgcggat
    14341 ttgccgctca taaaattgac gcagtgcgct ttcggaaacc gtttccgaac gttccagaaa
    14401 acggacgtac tcctcggcat aaaaagacgc ttcggcgatt ttaaagcggt tttggacatc
    14461 cttatcctta tccaaacctt ccttcaatgc cctgtttttc aaaacttcca aagtttgcag
    14521 ccgacggacg gcatcgtttc ggattgcctg cccgtccggt ttttgggact gctccgcatg
    14581 ccggtctgcc tgctgcatga tctgcgccac cagcgtatcc accaaagccg ggtcgatttc
    14641 gggtgctttg gctgccgcaa aacctgccaa cattgcagca ataactgcgg cagcggtttt
    14701 tttctgtttc atggtattcc ctgtcctgcg cggcggcaaa acgcctgtct gaacgtcttg
    14761 ccgccgtatc ccgttttcag aattattttg caggtttgat gtttgccttg cccaacagtg
    14821 caccgacggc acggtcaatc cgttccgcct gaaggttgcc ggcaatctgt cctttcattt
    14881 catcaaaaga aggcactttc acctcgcggc tgtcgttgac ataataaacg ccgtagaaat
    14941 cgccgttttt cagcggcgtt gccgtaaatt cgcctttttt caagtcctta attgcctgat
    15001 aaagcggcgg aacaccctgt tccaaatctt tcagcggcac atatccgacc ggcgcgccgg
    15061 tctgtttggt acggtcgttg agggaatatt gtttcaagac ggcatcgaaa ccttttttcg
    15121 ccctcaagtc ggcaaccgct ttttttgcat tttcttcctt gtcggtcagg atttcgccca
    15181 actggacttc ctgcgtacct ttgtaaaaac cgctgatatt gtcatatgcg gcttttactt
    15241 cctgctcgga aaccggttgg gttttggcga tatgcagcgc gtatgcctcg ccgttcaagc
    15301 catattttac cgcctgccaa acggttttga aggacggttt cttgtcgtcg cccgactttt
    15361 tcgcttcggc acgcaatttg gcaagcgcat ttttaaactc tgccgaccgg tcgagtttca
    15421 ggcgtttcac ttcctgtgcg accacggtgt tgaccacttc gttttccagc agggattggc
    15481 gcagttgcgg cgtgtcttcg gcacggctgt tttccgcacg gaatgcggca acctgcgcat
    15541 cgatgacgga actgtcgatt ttctgaccgt tgacggttgc cagcgtttgg gcaaacaggc
    15601 tgccggaaca ggcaagcagt gcaacggaag tcaggatttt tgctttcata atcttctctc
    15661 tttaaatttt aaattgaaat gttcgggcag acttcattca gtccgccgta tggaaatact
    15721 cgtcaggggt agccgcccgg atgccgagcg cgtgaatcat gccgcctgaa aacaaatctt
    15781 tgagcagcga tttgaccgtt ttctggcggt tcaggcggct tacgccttca aaacggccgc
    15841 taacgaccaa aacggcataa tgtccgccgc cggtattgcc cgcgtgtcct ttgtgcagat
    15901 ggctctcatc gccgatttcc aacaccagcg gatcgagcgt ctgcaggcgt tcgcggatca
    15961 aatcgacggc cggcattatt tgaacaccgc tttaaacggc ttgatcagca cttcgctgta
    16021 cacgcctgca tgaacatagg gatcgtcttc cgcccacgcc tctgccgcat ccaaagactc
    16081 gaactgcgcc acaatcaaac tgcccgaaac gcggttggag tcctccggca aaggattcgg
    16141 gcctgccgtc aacagccggc cttccgactt cagcgtctcc agccgtttga ggtgttcggg
    16201 acgtgccgcc atacgcgctt catacacatt ctccccgtct gttgccagca acataaaata
    16261 ttccacagtc aatcctcctt tttcagacag gtactcagat aaataccctg aataataaag
    16321 aaaacaagca tcagtgcagt cgagccgaac attttatagt tgacccattg cgactcgaac
    16381 cgggtaaaca caaaccagtt ggcgataccc ataaaaatca ggaaaccgac ccacatatac
    16441 gtcaatttcg cccatacggc atccggaagc tgaatctccc tgccgatact cgccttcaag
    16501 ccgtttttac cggcgaggtg gctgccccac aggaacagcg cgcccagcca aaacaaaacg
    16561 ctcggcttcc acataatgaa gcggctgtcg cccaaaacaa tggttgcgcc accgaatatc
    16621 acaatcagca ccagtccgac ccactgcatc gtatccagct ttttatattt ccaatacaga
    16681 aaagccgcct gaaccacacc ggcaaccaag gcgaccgccg ttgcggcaat catgtttttg
    16741 gtaacggtat aggtggcgaa aaacaggatg acggacaaaa ggtcgctgac gaatttcata
    16801 tccaaatgag aaagcaaatc aaataaacgc taatcataca ggcagaaccc tgtttttgca
    16861 cctgcaaaac caacaaattt tgttttgatt gatgcaaata tgcacagatg tttttgaaaa
    16921 aagatggaga tatgtcataa tttgtaaaaa cggcgaatga ttgccgttta aaatgtggcg
    16981 gcggtcggta cattcacata ttaaatgccc gccggccgac tgccccgccg atgcccgatg
    17041 atgacgaaat ttacagactg tacgcggtca aaccgtattc agccgccaac ccacagggga
    17101 tacatcttga aaaacaacag acaaatcaaa ctgattgccg cctccgtcgc agttgccgca
    17161 tcctttcagg cacatgctgg actgggcgga ctgaatatcc agtccaacct tgacgaaccc
    17221 ttttccggca gcattaccgt aaccggcgaa gaagccaaag ccctgctagg cggcggcagc
    17281 gttaccgttt ccgaaaaagg cctgaccgcc aaagtccaca agttgggcga caaagccgtc
    17341 attgccgttt cttccgaaca ggcagtccgc gatcccgtcc tggtgttccg catcggcgca
    17401 ggcgcacagg tacgcgaata caccgccatc ctcgatcctg tcggctactc gcccaaaacc
    17461 aaatctgcac tttcagacgg caagacacac cgcaaaaccg ctccgacagc agagtcccaa
    17521 gaaaatcaaa acgccaaagc cctccgcaaa accgataaaa aagacagcgc gaacgcagcc
    17581 gtcaaaccgg catacaacgg caaaacccat accgtccgca aaggcgaaac ggtcaaacag
    17641 attgccgctg ccatccgccc gaaacacctg acgctcgaac aggttgccga tgcgctgctg
    17701 aaggcaaacc caaatgtttc cgcacacggc agactgcgtg cgggcagcgt gcttcacatt
    17761 ccgaatctga acaggatcaa agcggaacaa cccaaaccgc aaacggcgaa acccaaagcc
    17821 gaaaccgcat ccatgccgtc cgaaccgtcc aaacaggcaa cggtagagaa accggttgaa
    17881 aaacctgaag caaaagttgc cgcgcccgaa gcaaaagcgg aaaaaccggc cgttcgaccc
    17941 gaacctgtac ccgctgcaaa tactgccgca tcggaaaccg ctgccgaatc cgccccccaa
    18001 gaagccgccg cttctgccat cgacacgccg accgacgaaa ccggtaacgc cgtttccgaa
    18061 cctgtcgaac aggtttctgc cgaagaagaa accgaaagcg gactgtttga cggtctgttc
    18121 ggcggttcgt acaccttgct gcttgccggc ggaggcgcgg cattgatcgc cctgctgctg
    18181 cttttgcgcc ttgcccaatc caaacgcgcg cgccgtaccg aagaatccgt ccctgaggaa
    18241 gagcctgacc ttgacgacgc ggcagacgac ggcatagaaa tcacctttgc cgaagtcgaa
    18301 actccggcaa cgcccgaacc cgctccgaaa aacgatgtaa acgacacact tgccttagat
    18361 ggggaatctg aagaagagtt atcggcaaaa caaacgttcg atgtcgaaac cgatacgcct
    18421 tccaaccgca tcgacttgga tttcgacagc ctggcagccg cgcaaaacgg cattttatcc
    18481 ggcgcactta cgcaggatga agaaacccaa aaacgcgcgg atgccgattg gaacgccatc
    18541 gaatccacag acagcgtgta cgagcccgag accttcaacc cgtacaaccc tgtcgaaatc
    18601 gtcatcgaca cgcccgaacc ggaatctgtc gcccaaactg ccgaaaacaa accggaaacc
    18661 gtcgataccg atttctccga caacctgccc tcaaacaacc atatcggcac agaagaaaca
    18721 gcttccgcaa aacctgcctc accctccgga ctggcaggct tcctgaaggc ttcctcgccc
    18781 gaaaccatct tggaaaaaac agttgccgaa gtccaaacac cggaagagtt gcacgatttc
    18841 ctgaaagtgt acgaaaccga tgccgtcgcg gaaactgcgc ctgaaacgcc cgatttcaac
    18901 gccgccgcag acgatttgtc cgcattgctt caacctgccg aagcaccgtc cgttgaggaa
    18961 aatgtaacgg aaaccgttgc cgaaacacc
//
LOCUS       DAWKST010000021        18845 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.021, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000021 DAWKST010000000
VERSION     DAWKST010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18845
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.021"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            52..1005
                     /locus_tag="WJL87_000512"
     CDS             52..1005
                     /locus_tag="WJL87_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ7798982.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVSKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHNVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1109..1432)
                     /locus_tag="WJL87_000513"
     CDS             complement(1109..1432)
                     /locus_tag="WJL87_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798983.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1605..2966)
                     /locus_tag="WJL87_000514"
     CDS             complement(1605..2966)
                     /locus_tag="WJL87_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ7798984.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSGKLGFELSEETTAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPAPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASQEQRTEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVEGT
                     DWE"
     gene            complement(3327..5027)
                     /gene="recJ"
                     /locus_tag="WJL87_000515"
     CDS             complement(3327..5027)
                     /gene="recJ"
                     /locus_tag="WJL87_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ7798985.1"
                     /translation="MSVKIQTRSINTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLAALLPYQTLTNCEAAAGRLADAVGRKEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPADTVPDCIIVNPNQKGCGFQSKSLAGVGVIFYVLTALRAELRRRNYFSDGLKEP
                     NLGDLLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDSEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQTTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTTFEEAVRE
                     MVCEDDLSQTFITDGSLPACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKVWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAV
                     "
     gene            complement(5079..5813)
                     /locus_tag="WJL87_000516"
     CDS             complement(5079..5813)
                     /locus_tag="WJL87_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7798986.1"
                     /translation="MLFRKTTAAVLAATLMLNGCTLMLWGMNNPVSETITRKHVDKDQ
                     IRAFGVVAEDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKPFQIVEDTPS
                     YARHQALPVKLESPGSQNFSTDGLCLRYDTDRPDDIAKLKQLEFEAVELDKQTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTEKHTDKSKLFANILYTPPLFDTGCG
                     GRGTGFACGGSGCGRGCRPQMNSNAV"
     gene            complement(5860..6366)
                     /locus_tag="WJL87_000517"
     CDS             complement(5860..6366)
                     /locus_tag="WJL87_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ7798987.1"
                     /translation="MFEQEDEWVSKTQMKKQMNGLQDLGMELTKLSSDTLKKIGLDED
                     LYEAVVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQRVE
                     RTRMELLADDGALTQFMSDFPHADAGKLRTLIRNTKKEQEQNKPPKNFRALFQELKTV
                     MENGDAEI"
     gene            6521..7852
                     /gene="pmbA"
                     /locus_tag="WJL87_000518"
     CDS             6521..7852
                     /gene="pmbA"
                     /locus_tag="WJL87_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ7798988.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADPELMAQHIGDPDLYHEWDLDTEAAVGFAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(8149..8352)
                     /locus_tag="WJL87_000519"
     CDS             complement(8149..8352)
                     /locus_tag="WJL87_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ7798989.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            8595..8897
                     /gene="clpS"
                     /locus_tag="WJL87_000520"
     CDS             8595..8897
                     /gene="clpS"
                     /locus_tag="WJL87_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ7798990.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            8899..11196
                     /gene="clpA"
                     /locus_tag="WJL87_000521"
     CDS             8899..11196
                     /gene="clpA"
                     /locus_tag="WJL87_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ7798991.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDEDEDEDDGNYSSDG
                     MDDDNENRAKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPL
                     LVGEAGVGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKS
                     VLKQLEKIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEY
                     RTIFDKDHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELS
                     ARYINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVSRIPEKTVSH
                     DDKQVLQFLGRDLKNMVYGQENAIDALVAAVKMSRSGLALPDKPIGSFLFSGPTGVGK
                     TEAAKQLAYSLGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHC
                     VLLLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSL
                     GFTAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHQLLD
                     KKVEAEFTSALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSDGGFVRI
                     DWDAAKEEAVLKFKKSKVKIKTASA"
     gene            complement(11260..12264)
                     /gene="rfaD"
                     /locus_tag="WJL87_000522"
     CDS             complement(11260..12264)
                     /gene="rfaD"
                     /locus_tag="WJL87_000522"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ7798992.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LNWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVTFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYKEEFFDVKSGVDRYVKWMLENLA
                     "
     gene            complement(12305..13423)
                     /locus_tag="WJL87_000523"
     CDS             complement(12305..13423)
                     /locus_tag="WJL87_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ7798993.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPLIQKNCFFNKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMPQCPFTLITDEVLLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKNKMRIYPEGREKYLEYHRKFVFQDNIDDFL"
     gene            complement(13425..14660)
                     /gene="dcm"
                     /locus_tag="WJL87_000524"
     CDS             complement(13425..14660)
                     /gene="dcm"
                     /locus_tag="WJL87_000524"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ7798994.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFSRVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(14697..15668)
                     /gene="hldA"
                     /locus_tag="WJL87_000525"
     CDS             complement(14697..15668)
                     /gene="hldA"
                     /locus_tag="WJL87_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ7798995.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(15723..16463)
                     /gene="pyrF"
                     /locus_tag="WJL87_000526"
     CDS             complement(15723..16463)
                     /gene="pyrF"
                     /locus_tag="WJL87_000526"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ7798996.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            complement(16989..17636)
                     /gene="adk"
                     /locus_tag="WJL87_000527"
     CDS             complement(16989..17636)
                     /gene="adk"
                     /locus_tag="WJL87_000527"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ7798997.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            17851..18690
                     /gene="htpX"
                     /locus_tag="WJL87_000528"
     CDS             17851..18690
                     /gene="htpX"
                     /locus_tag="WJL87_000528"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ7798998.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGTGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
BASE COUNT         4601 a         5114 c         4523 g         4607 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgctaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgc
      361 accaagcgcg gcagcatcgg cggcagaacg ccacggcaga acggctatat ccgcgcccta
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatcttgcc
      481 gtcgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat tttagtgcgg
      541 cctgccgtcg aagccggcga gaaactgggc tttctgcccg gagacctgac ccaaaaagtc
      601 gacccctacc tccgtccgct ttatgacgcg ctttacgacc tgatgggctt cgaccgcgtc
      661 agcaaactga ttgaaaaagg cctgattgaa atcgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 aacgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctctg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa tcgggcagag aaaaacggat acggaaacca agctccgtat
     1081 ccgtttcttt atccgtccaa ccgatacatc aatcgaattt tctgcatcgg attgcctcat
     1141 cttcgcaaaa cattgccgaa gtttcgcggt tgcacgcctg acgcactttc tgccgcgcca
     1201 cgccttcatt ccgatcgctt gcctcaaacg tcttctgaaa aggcgtaacg ctgcatacat
     1261 acatggtgga atttgaaccg aaaaggtcga ttttgacggt attgctgccg ctttccaata
     1321 cggcgacacg gtgttccaac tgcgccagac gtgcttccaa atcgccgatt ctgtcggcaa
     1381 atgcaggcat ggcggcacac gcggtcaaaa tgcccaaaat acattttttc atcaattttt
     1441 ccttgtttct tgaaccctac ccacccggac atcctttttc cgagaggcag ggtcagactt
     1501 tatttacggg gatgaatccc cttacaaatc aggataatac atagaacggc actttatgct
     1561 tcatcccatg tgttgaaact tcattgctcc ttgtctgttg acccttattc ccaatccgtt
     1621 ccctcaacct tcggcttggg tttgcgcctg cgacggcggc gttttttcgc ctgtccttcg
     1681 tttttttcgt gccgggcagc agcctcgttt ttggtcattt ccgtccgttg ttcctgcgat
     1741 gccgtctgaa acgccgtcca ccactcggca agggcgcggt ccgcattgcc ggtttcggcg
     1801 cgcaagagca ggaaatcata ggcggcacgg aaacgcgcct gtgcaaacag tttatgcgga
     1861 cgtgcgcctt tgcggttctc aaactgcggc tgaaacatcc aaatttcgcg catcgtggcg
     1921 gaaaagcgtt gcggcacgcc ccaaccgcgt tcgacggttt cacgcatcgt attgattgca
     1981 tcggacaggg cgggcgcagg tttcaaaccc tgttgcagat tgcttttcca atggcgttcc
     2041 aactcgggcc acatcagagc cgccagtaca aaaccgaccg aaaccgattt gtcagcacgc
     2101 agccgctcat cggtattttt cagggcaagc accgtcattt ttccggcgat gccgtctgaa
     2161 acgcgcaagg cattgagcag cggatggatg tcgtccggta tgtcaaatcc gttcaaacgt
     2221 ttcagacact cgcgcgcgtg ccctgaaaac agcagtttca tgatttcgtc aaacagcctc
     2281 gctaccggtt cgtgcttcag acggcatatc gattcggcaa tcggtgcggt ggtttcttcc
     2341 gacagctcaa agcccaattt gcccgacagg cggatggcgc gcaaaatcct gacagggtct
     2401 tcctgatagc gttcggcggc atcgccaatc ataaccagcc tgcgggcggc aacatcggca
     2461 atcccgttgt ggaaatccaa aatctcttct ttttcaggat cgtaatacaa ggcattgcag
     2521 gtaaaatcgc gccgcatcgc atcttcttcg atgctgccgt aggtgttgtc tttcatgatt
     2581 ctgccgtgcg cgttctgatg aaccttcgca ccgccgcgaa acgtcgttac ttcgataatc
     2641 tccgcaccgt tcatcacatg gacgatttga aaacgcctgc cgatgatgcg gctgcggcgg
     2701 aagagtttgt gcacctgttc gggcgtggca tcggttgcga catcgaaatc tttgggttcg
     2761 atgccgagca gcagatccct gaccgcgccg ccgaccacat aagcctgaaa ccccgcccct
     2821 ttcagacggc gtatgacgtt ttcggcggca aagctcaaca tttcggcacg gatgttgtgt
     2881 ctttcggcag gaatgaccgt tttactttcc gcttttttac tgctccgacc ggaaggcagc
     2941 atcttattca accatttttt cagcataaat attctaaccc gaagagcggc atctccacgg
     3001 gcaaccgtgt tcagacggca tcaggcggta cgattccgca agatgccgta accataaaca
     3061 aaaggcgtag aaactacgcc gtccgaatca cgccgccctc gcggcggcgg caacgcgcca
     3121 ttataacaga ttgcccttta aaaaaatcag accctgcttt tgtggcggag tctgaaattt
     3181 gatgcacctt atatattgcg tccctaagaa gggatgatta acaaaaatta acgcccttta
     3241 ctttcttttc cgattgggaa cattcccgtc gtacaaacag gcaaatgaag gaacagaaat
     3301 cacattcaaa cggcgttccg ccgcctctac accgcttccc agtaatcgat atacagctgc
     3361 aattcgagat tgttgcgcca ttcgttggca acggggcggt aaaccgtgcg gatgtattcg
     3421 gggatgtctt cgctgcaacg ccaaaacatc gcttcaaatt cgcagccgtc tttttgcagc
     3481 cagacttttt tgtgtttgcc ctccgcgccc aaaggttgct ggcggacgac gtggaactcg
     3541 tcggtaaagc tcggcggcgc gaagccctgc ccccaaacgt gacgggcaag gttttgcgcc
     3601 tgttccaacg tgatgtcgca ggcgggcagg ctgccgtcgg tgatgaaggt ttgcgacaaa
     3661 tcgtcttcgc acaccatttc gcgcacggct tcttcaaagg tcgtctgaaa cgcgggaatg
     3721 ttgtgttcaa gtatgctcaa acccgccgcc atcgcgtgtc cgccgaattt taaaatcaaa
     3781 tcgggatggc gtttggacac caagtccaaa gcatcgcgca ggtgcaggtt gggaatggaa
     3841 cgccccgaac cgcgcacttc gccgttgtcg gcaggcgcaa acacgatggt cggacgataa
     3901 aaacggtctt tgaggcggct ggcgacgata ccgaccacgc cttgatggaa gtcgtcgcga
     3961 tacgccacca aagtcgtctg acctgaaggc agggtttcag gaaaggcgtt cagcgcgtct
     4021 tgcagcatag actgctcgat ttcgcgacgc tcgatattga ggttgtttaa ctgagccgcc
     4081 agttcctgag cttcggaatc atctcgcgcc aacaggcagg cgatgccgac cgacatatcg
     4141 tccagccgtc cggcggcatt gatgcgcggg cccaacgcaa aacccatatc aaacggctgc
     4201 gccttgcgcc agtcgcgccg cgccacttca aacaaggcgc ggatgccggg gcgcattttg
     4261 cccgaacgca tccgtttcaa accttgcgac acgaggatgc ggttgttgtg gtcgagaggg
     4321 acgacatcgg caaccgtgcc gagtgcgacc aaatccaaaa gatcgcccag attcggctct
     4381 ttcaggccgt ctgaaaaata attgcggcgg cgcagttcgg cacgcaacgc cgtcaacaca
     4441 taaaaaatca cgcccacgcc cgccaagctt ttgctttgga aaccgcagcc tttttggttc
     4501 ggattgacga tgatgcagtc gggcacggta tcggcaggca gatggtggtc ggttacaatc
     4561 acatccaaac ccagagcctg cgccctcgcc acgcctgcga tgctggcgat accgttatcc
     4621 accgtaatca gcaaatccac gccctgcgcg gcagcgattt cggcaagttc gggcgttaag
     4681 ccgtagccgt gttcaaagcg gttgggcaca aggaaatcca cttttgcccc catcgccgcc
     4741 aaaccgtcca aaccgacggc acacgccgtc gcgccgtcgg catcgtagtc ggcgacaatc
     4801 aggatttttt ccttgcgccc aaccgcatcc gccaaacgtc cggcggcggc ttcgcagttt
     4861 gtcagcgttt gatacggcaa aagcgcagca agcttgtcgt ccaattcggc aggactttgc
     4921 acaccgcgcg aagcacaaag ccgggcgatt aaaggatcgg caccggcggt gagcaaatga
     4981 ttaaaaacgt cggtattgat ggatcgggtt tggattttga ctgacataac gtggggtttt
     5041 actgtgcgtg tgtgcttaaa atgccgtctg aaagcttttc agacggcatt gctgttcatt
     5101 tgcgggcggc atccacgacc gcacccagag ccgccgcagg caaagccagt accgcgcccg
     5161 ccgcatccag tatcaaaaag ggggggcgta tataagatat ttgcaaacag cttggatttg
     5221 tcggtatgtt tttcagtaac cgtgtaataa atatcggcag gcacactttg ctcaaaatga
     5281 taatcggcgt tcagtttttg cggcgtggcg tagtatttgc ctttggcaga gacgcagcgc
     5341 gtgtaaatag tctgtttgtc gagttcgacc gcttcaaact caagctgttt cagcttggcg
     5401 atgtcgtcag gcctgtcggt atcgtagcgc aggcaaaggc cgtcggtact gaaattctgg
     5461 ctgccgggcg attcgagttt gaccggcagg gcttggtggc gggcatagct cggggtatcc
     5521 tcaactattt ggaagggctt gtccagcccg gccttcaaaa tgcccgtcag cttcgccgaa
     5581 tcttcaggat tgacgacgaa ccagtatttt ccgcccatca tcaccaggct gcccttttcc
     5641 aattgggcat tgtcttcggc aaccacaccg aaggcgcgga tttggtcttt gtcaacgtgt
     5701 ttgcgggtga ttgtttcgct gaccgggttg ttcattcccc acaacatcaa cgtacagccg
     5761 ttcagcatca aggttgccgc caaaacggcg gcggtcgttt tacggaacaa cattttatcc
     5821 tccaatctaa ataacggcgg atgtcgtctg aaaatatgcc taaatttccg cgtccccgtt
     5881 ttccatcacg gttttcaact cttgaaacag ggcgcggaaa ttttttggtg gtttgttttg
     5941 ctcctgctct tttttggtat tgcggatgag tgtcctcagc ttgcccgcgt ccgcatgcgg
     6001 aaaatccgac ataaactgcg tcaacgcgcc gtcgtctgcc agcagctcca tccgcgtccg
     6061 ctccacacgt tgcaaaaagg cgttgtgcgc cgcatcgtcg ccgcgcagct tggcaagaaa
     6121 tgcctcgatg ggcgcgggat cggtatcgcg catcagtctg ccgataaatt gcgcctgccg
     6181 cttgagcgcg ccgttggatg tgattttttt ataggtaacg acggcttcgt acaaatcttc
     6241 gtccaagccg atttttttca acgtgtcgct tgagagcttg gtcagctcca tacccaaatc
     6301 ctgcaaaccg ttcatctgct ttttcatttg ggttttgctg acccattcgt cttcttgttc
     6361 aaacatcttc attctcaaat tttatccgac gggcgcattt taacagaatg attccgcgtt
     6421 cagacggcat tttgcaccgt ccgccctgca aacggcacgc ttttgcccct gccgcccgaa
     6481 acggttaaaa tgccgcaacg ctccaccaag aaagatttct atgctgttca accacaccgc
     6541 ttccgaactt ctcgacctct gccgccgcac gctcgacttg gcaaaagcag cgggcgcgac
     6601 cgccgccgaa gccgatttca gcgaatccat cggacaaagc gtcagcgtgc ggctgggcga
     6661 aatcgaacaa atcgagttcc agcaggacaa gtcgctggac attaccgttt acgtcggcaa
     6721 acgcaaaggc cgcgctggca ccgccgattt ctccgaacaa gccctgcgcg acaccgtccg
     6781 cgccgccgcc gacatcgccc gccacaccgc cgaagacggt tgcgccggac ttgccgaccc
     6841 cgagttgatg gcgcaacaca tcggcgaccc cgacctttac cacgaatggg atttggatac
     6901 ggaagccgcc gtcggctttg caaaacaatg cgaacaagcc gccctgaacg aggatgagcg
     6961 catcgaaaac tccgaaggcg cggcggtgca aaccggccat taccaatacg tttacggcaa
     7021 cacccacggt tttgccgcac accggcaagg cacgcaccac agcatttcct gcagcgtcgt
     7081 tgccgccgac gaaaacggga tgcagcgcga ctactggtac gattccgcct gccgccatcc
     7141 ggatatggac agccccgaaa ccatcggtca aaccgccgcc cgccgcactt tgcgccgact
     7201 cggcagccgc agcatcccga caggcagcta ccccgtcctc ttcgatacca ccgtttcggg
     7261 cggccttatc ggacacctcg tcggcgcact ctccggcggc gcgctctacc gccaaagcag
     7321 tttcctgatc gacagcatcg gcaaaaaagt cctgcccgat ttcctcaacc tgcgcgaaga
     7381 accgcacatc ccccgctctt tccgcagcag ctattttgat gcggaaggcg ttgccaccgc
     7441 accgcgcttc gtgattcaaa acggcattgt cgaaggctat ttcctcagca gttacagcgc
     7501 gcgcaaactc ggtatgcaga ccacgggcaa cgccggcggc gcgcacaacc tgtatttgaa
     7561 ccatacgcac gaaacgcaat ccgacctgct gaaagaaatg gggacgggat tattggttac
     7621 cgaactgatg ggacagggcg cgaacaccat taccggtgac tactcgcgcg gcgcggcggg
     7681 tttttgggtg gaaaacggcg tgattgccta ccccgtccac gagattaccg tagccggacg
     7741 cttgcaggat atgtaccgcg acattgtcgg cgtggcggat gacgctttgc ggcgttcgtc
     7801 caacaaaatc ggctcgattc tgattgcggg gatgacggtt gccggaagct gaacgccctg
     7861 ccgatgccgt ctgaagacga aggcagcacg ttttgccccg tgcctgcccg tttgccgttt
     7921 tatgccgcac cgccttttgc cgacaacatt gcacattttt tcatcagcac tgattttccc
     7981 ttatccgtca aaatactaag ccacttgtcc acaacagggt acacacaagt tcaggataag
     8041 gcgtgcgggg attggggttt ttccgccgcc ggcaaaacgg cagacaaaaa cggcgggaca
     8101 ttgcctgtcc cgccgttttc aaatatacat ttgaccgtac atcaggaatt aagcagcctg
     8161 aatgttggcg gcctgtttgc ctttagggcc ggtggttacg tcgaaagaga cgcgttggcc
     8221 ttctttcagg gttttgaaac cttccatatt gattgctgag aagtgagcga acaaatcttc
     8281 gccgccttca tcaggcgtga tgaaaccaaa acctttagcg tcgttaaacc attttacgat
     8341 accggttgcc attagaaact tcctatactc aaaaattaac aaaatcagca aaacaaggca
     8401 tacggcaaac cgagccgtct ggcgttcctt ccagcttgat atactcggcg gatgcccaat
     8461 cctgcttaac cgtcttttta catttgttaa gacaaaacgt caagctatgt tttcaaaaaa
     8521 gtgggaaaat aggcaaaagt accgaaaata caacaaaaat gccgtctgaa cgcggctttc
     8581 aaatgggaca cactatgacc gcgcaacacc aatccgacac gctcttgcac cgtctgaaca
     8641 ccctgccgcc gaaacgttac ggcgttttcc tattgaacga cgattacacc acgatggaat
     8701 ttgtcgtcga aatcctgacc gaaatcttta tgctcggaca agaacaggcg gtagcggtaa
     8761 tgctcttggt tcatcacgaa ggcaaaggcc tgtgcggcac ttacacgcgc gatattgccc
     8821 aaaccaaaca acaacaagtc atgcagcggg caaaagccga agggcatccg ctgcaatgta
     8881 ttgtcgagga gatttaatat gcttgcaccc gaattggaac agattttgca gcagctttac
     8941 cgcgaggcgc gtaaggctca ttatgaattt atcagcctcg agcatctgct tttggtactc
     9001 atcgaagaag atgcctccgt gccgaacgtc ttaaaactat gcggcgcgga tttgaaagtg
     9061 gtgtccgaac agctcgccgc cagcgttgcc gaaaacaccc ccctgattcc cgaacacctt
     9121 ttagacacgg tcgaaacacg gcccacgctc ggcttccaac gcgtgatgca acgggcgatg
     9181 gtgcataccc agtctgccgg aaaagccgca gtcgaaccgt tggacgtttt ggtcgcgctg
     9241 atgagcgaaa ccgacagcca caccgtctat ttcctcaagc tgcaatcggt tacgcgtttt
     9301 gaagttttgc gctgtattgc ccacggctct cccgatgaag atgaagatga agacgatggc
     9361 aactattctt cagacggcat ggacgacgat aatgaaaacc gcgccaaacc gggcaaaaac
     9421 cctttatcgg cgtacaccgt caacctcaac gccgaagtca aagccggccg tatcgaccct
     9481 ttgattggtc gcaaacacga aatggaacgg ctggtgcaaa tcctatgccg ccgccgcaaa
     9541 aacaatccgc ttttggtcgg cgaagcgggc gtgggcaaaa ccgcgctggc ggaaggtttg
     9601 gcacatcaaa tcgtcaacgg cggcattcca gacgcgctca aagatgccga agtgtacgcg
     9661 ctggatatgg gctcgctgtt ggcgggcacg aaataccgcg gcgactttga agcgcgggtc
     9721 aaatccgtct tgaaacagct cgaaaaaatc ccgcacgcca ttttgtttat cgacgaaatc
     9781 cacaccatca tcggcgcggg cagcaccagc ggcggcacca tggacgcgtc caacctgctc
     9841 aaacccgcgc tggcaaaagg ttcgctgcgc tgcatcggcg cgaccaccta cgacgaatac
     9901 cgcaccattt tcgacaaaga ccatgcctta agccgccgct tccaaaaaat cgacgtggtc
     9961 gaacccaccg tttccgaaac cgttcaaatc ctgcgcggct tgaaaccgat gtttgaagcc
    10021 ttccaccaag tccgctacac tcaaggcgca ctcgaagccg ccgccgaact ctccgcacgc
    10081 tacatcaacg agcgtttcct gcccgacaaa gccatcgacg taatggatga agcaggcgcg
    10141 gcgcaacgga ttctgcccaa atccaaacag aaaaaagtca tcggcaaagc acaaatcgaa
    10201 accgtcatcg ccaaagtttc gcggattccc gaaaaaaccg tgtcgcacga cgacaaacaa
    10261 gtgctgcaat tcctcggtcg cgatttgaaa aacatggttt acggtcaaga aaacgccatc
    10321 gacgcgctgg ttgccgccgt caaaatgtcg cgttccggcc ttgccctgcc cgacaaaccg
    10381 ataggcagct tcctcttctc cggcccgacc ggcgtgggca aaaccgaagc cgccaaacag
    10441 cttgcctact cgctgggcgt accgctgcaa cgctttgata tgtccgaata catggaacgc
    10501 catgccgtat cgcgcctcat cggcgcgcca ccgggctacg tcggctttga acaaggcggc
    10561 cttttgaccg aagccgtcaa caaacagccg cattgcgtat tgctcttgga cgaaatcgaa
    10621 aaagcccacc ccgacatttt caacgtcctc ctgcaagtca tggacgcagg caaactgacc
    10681 gacaacaacg gcaagagtgc cgatttccgc aacgtcatcc tgattatgac caccaacgca
    10741 ggcgcggaaa gcctcagccg ccccagcctc ggctttaccg ccaagcgcga acgcggcgac
    10801 gaaatgcagg caatcaacaa gctcttcacg cccgagttcc gcaaccgctt ggacgcgatt
    10861 atcccgtttg cacccttgtc cgaacccatc atcgtcaaag tcgtggacaa attcctgctc
    10921 cagctcgaac accagctcct cgacaaaaaa gtcgaagccg aattcacgtc ggcattgcac
    10981 aaatatctgg cggaaaaagg tttcgacccg caaatgggcg cgcgcccgat gcaccgcctg
    11041 attcaggaaa aaatccgcaa accgctcgcc gacgaactcc tgttcggcaa actatccgac
    11101 ggcggcttcg tgcggataga ctgggatgcg gcaaaagaag aagccgtgtt gaagtttaag
    11161 aaaagcaagg tcaaaataaa aaccgcctcc gcgtaaaatc aaaaatgccg tctgaaattt
    11221 cagacggcat tttctttcaa acaaaattac caatcgtgtt taagccaaat tttccagcat
    11281 ccatttgacg tagcggtcga cgcctgattt gacatcgaaa aattcttcct tatatccggc
    11341 ttcgcgcaat ttggtgatgt cggcttgggt gaagctctgg tatttgcctt tgagtgcgtc
    11401 ggggaagggg atgtagcgga taagttcttc ttccaccagc tctttcaagc tcatttcagg
    11461 cttgccttcg gcggcgcggc aggcgttgac ggtggcggcg gcgagttcgt taaactgttg
    11521 gctgcggccg gtaccgaggt tgtagatgcc ggaaagttcg ggatggtcga agaagtagag
    11581 gttgactttg gcaacgtctt cgacgctgac aaagtcgcgg gtttgttcgc cgttgccgta
    11641 accgtcattg ctgccgaaca ggttgacgta accgtgttcg cggtattggt ggaaatggtg
    11701 gaaggtgacg gatgccatgc ggcctttgtg ttgttcgtgt tgtccgtaaa cgttgaagta
    11761 gcggaagccg acgacttggg cggtgagacc ttctttcatg cggcggcgca atacttggtc
    11821 gaacaggaat ttggagtagc cgtacacatt aagcggtttt tcgagttcgc gctcttcgcg
    11881 gaagatttcg cctttgccgt aaaccgccgc actggaggca taaaggaagg gaatgcgttc
    11941 gtcctgacac cagttcaaca aatccagcgt gtactggtag ttgttttcca tcatatacaa
    12001 accgtcgtgg ttcatcgtat cggaacacgc gccttgatgg aaaacggctt cgatgttttg
    12061 ataaggtaaa atgtgttccc tcacttggcg gatgaattcg tgtttgtcga gataatgggc
    12121 gatttcgcac tcggcaaggt ttttgaattt ttcgcctttg ctcaaattat cgacggcgac
    12181 gatgtcggta ataccgcgtt gattaagtgc tttgacgatg ttgctgccga taaagccggc
    12241 cgcgcctgtt acgatgatgg tcatattcgg tttcctttgt tttgagtaaa attaataaac
    12301 ttagttacaa gaaatcatcg atattgtcct gaaacacaaa tttgcgatga tattccaaat
    12361 atttttctct tccttcggga tatattctca tcttattttt tgcatttgga ttgatattaa
    12421 gtttttccca tgtgtaacgg cttagtcgag taccgcaaat caaacgacca tcatccaaga
    12481 aagtaatata accttgatcg aacaaccaat cataagtcgg cgttaaagct aacccattta
    12541 aataatctaa tgcctctttc tcatttttct cagtaataca aaccatataa ggtttaatat
    12601 gactagccct taacaaaacc tcatctgtaa ttaatgtaaa tggacattgc ggcatatgat
    12661 ttataacatc cttgcgatat ttctgctgcc cttttctact tttctgttta atgatttctt
    12721 gctctacgaa ttgattaaat tctgaagccg gtatttctaa tttttctctt gataagagtt
    12781 gcgggtgcac gatagagcga aattgataat ccaaaaatat tcggaaataa aataatggtt
    12841 ctgaatcttc tatatctttg acaggcaata gtttaagaat tgataaataa ctaattttgg
    12901 gcaatacaat cttacgccaa attgaccata tatcatcctc cgaacggata taacccctac
    12961 ggtaaccttt agaattttcc aaatggtctg atacatcatg gattgaaaag aaaatatttt
    13021 ctgtaggcag attattaata cttaaaacgt aatcatcata aaaccttgaa atatcatgat
    13081 gatatttctg ttcttgtttg tgatattcaa cttttgcatc aaccaaataa cgctgtaaat
    13141 ttttcttatt aaaaaaacaa tttttctgaa tgagaggata ttctaccctc cttccatttt
    13201 ttgtctcagt aatcccccaa ttttcatact caaaaaatgt acttagtttt cgaccactat
    13261 catcaatata actacctaca tatttgcgtg cttccccatt accaccaaaa acagataact
    13321 tgttattcct gtgaataaaa ctatcttccg cacgtaaatt ttgaatacta tcgacaataa
    13381 aatactctat gttttcaata gtaattcttt gaaaatccgg cattttactc tccataaagc
    13441 gtaatatcca tttgttttaa tatagcgatt aatacatcaa caacaatact gtttcccgct
    13501 tggcgataca tttgagtatc actaactaaa attttaaaat catctctaaa acccattagc
    13561 cgcaaacatt ccctaggtgt taatttacga atacgtccac gattatgagt aacataatta
    13621 tcaaccccgg cacgatgcat tttatgcata gtttgcaata atgggcgagc tacaggcaaa
    13681 tcagtttccg tactggtttt aaaatttttt gttcctcctg ccaaaacata atttttgatt
    13741 ttttcagaaa gataatattt ttcctcaacg ttatttacat caaaaataaa gtcatcaaat
    13801 tcagattcgc gggctgcctg aaaaataaaa tcaccatgcc aattaaattg ttgatttgct
    13861 ttttggcata aggcaatttc tccattaatc tgtgtataac gtttttgtct atttttagaa
    13921 ctggtaacaa atttcacacc cttttcacgt aaaaaatatt tgctatcagt atagtcctcc
    13981 aaaaaatctt gcatagtatg ttctaattca atcttttcag gaaactgaaa accatttata
    14041 ggaggggtat gaaagccaac aacaaaaata cgctcacgat gttgaggaat cccataatcc
    14101 ttactattca ttatttggaa atataagtca taaccaagtg aataaaaaac actttttaca
    14161 actttccaag tttttccatt atcatgattg agcaagccct ttacattttc ataaataaat
    14221 atctttggtt ggacttcatc cacaacgcga gaaaattcat agaataatgt tcctcgtgta
    14281 tcttctaatc ctgcacgttt gccaaccatg gaaaatgctt ggcaaggact gcctccaact
    14341 aaaatatcaa cttgatttct aaactttctc gcatcaaatt gagtaatgtc gttatgccaa
    14401 aaatcttcat ttaatttata gtttccaaga taactttttt taacgtatgg atcaatatct
    14461 cctgcaaaaa caatggtatg gtttaaattt aatcggtgga atgcctgttc aaccgcacca
    14521 atcccgctga atacggttgc taatctaata tgtgaatcag gtttaagaaa agttttagat
    14581 ttccaacctt gttgactggg aaagagcaaa gttttttgta atcgagtatc gtgtgtctgt
    14641 gccattgtcg aaatagtcat acttatatcg ttctgtttat cttatcaata tgaaaactac
    14701 attgttgatt gccctgacaa tgccttgatc aattcggcaa acgagcaaac cgccgtaccg
    14761 agtttcgcca cgacaacccc ggccgcagta ttggcaaggt acatggcttc gggcatggtg
    14821 cagcctgccg ccaaacccaa gcccattccg gcaatgacgg tgtcgcccgc accggatacg
    14881 tcgtaaactt cttgggcgcg ggtgggctgg taaatcggtt cgccttcgct gaacaaggtc
    14941 atgccttctt cgcttcgggt cagtaaaacg gcggtcaggt cgaggtggcg gcgcaggttt
    15001 tgcgcttttt cggtcagctc gctttcgttt ttccaactgc cgaccacttc tttcaattcg
    15061 gcgcggttag gcgtaatcag agttgcaccg acatattttt cgtaatcgtc gcctttgggg
    15121 tcgattaaga cggttttgcc ggcgtgtttc gcccaatcga tcatatcgga gatatgcgac
    15181 aggccgcctt tgccgtagtc tgaaaaaatg attgcgtcgt attcgggcaa gatttcgcgg
    15241 tatttctgct tgatttgttc caacacttcg cagttgggat gttcttcaaa atcaagacgg
    15301 ataagctgct ggttgcgggc gacgacgcgc agtttgacgg tggtggcgat ttgtttgtcg
    15361 cgcatcagat aggaggcgac gccgtcctgc accatcagcg catcgagcgc gtcggcggct
    15421 tcgtcgttgc cggttacgga caacagccct gccctgccgc ccaacgaagc gatgttgcgc
    15481 gcgacatttg ccgctccgcc cgcgcgttgg tcgattcgtc cgattttcgc caccggcacg
    15541 ggggcttcgg gcgaaatacg ggacacatcg ccgaaccaat agcggtcgag catcacgtcg
    15601 ccgacaacca ggactttggc ttgcgcgaaa cgggatttga gggtttcttg ttggaacttg
    15661 gcggacatat tgcctccgtt gtattttttc agacggcctc agcctgtaag gccgtctgaa
    15721 aatcagtttg cttcaaggtt tgccacgcgg ttcacttcgc gcaatacggc taccggatcg
    15781 gcagcttggg ttacaggacg acccattacc aaataagtcg aaccggcagc caaggcttcg
    15841 gccggtgtca tgatgcggcg ctggtcatca ttattgccgg caacgtccaa gcggatgccg
    15901 ggcgtgacca agacaaaatc ctgtcccaat tcgcggcgca gcggcgcggc ttcttgggcg
    15961 gaacagacca cgccgtccaa gcccgaactt tgcgccagtt ttgccaagcg gatgacttgt
    16021 tcttcagggg cggtgttcaa accgatttcc gccaaatcgc tttgttccat gctggtcaac
    16081 acggttacgc cgattaagag cggcttcgtg ccgtatccgg caacggcttc tgctgcggct
    16141 tccatcatac ggcggccgcc cgatgcgtgc atatcgacca tccaaacgcc catatcggca
    16201 gcgactttgc aggcttgcgc gacggtgtgg ggaatatcgt ggtatttcaa atcgaggaaa
    16261 agtttgaaac cttgattgat taagctttct gccaaattgc gtcccgtcgc ggtaaacagc
    16321 tctttgccga ttttgatttg acacaatgtc gggtcaaggt tgcggacgaa tccgagcgtg
    16381 tctttttcgt tggaaaaatc aagggcgacg ataacggggg tgcgttgttg cggagtttgg
    16441 aagtcggaga ttaaaggatt catggtgttt tcgtttcggg ttttcagacg acctttatcg
    16501 tctttttcgc agtatagtgg attaacttta aaccggtacg gcgttgtctc gccttagttc
    16561 aaagagaacg cttctctaag gtgctgaagc acaaagtgaa tcggttccgt actatctgta
    16621 ctgcctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactataac aacgtaaatt
    16681 caaaccgtag gtcgggcatt gatgtccgac ctacgcgaac tcaagcctcc gacaaactgt
    16741 ttcggataaa tttgaaccgg tacatcagcc gtcattcccg cgcaggcggg aatccattga
    16801 taaaactcag ttcatcagat ttaaacagcg attgcccaaa ttcagaaatg gattcccgcc
    16861 tgcgcgggaa tgacgccaaa tgattatttt tcggttgcaa cacgctgttt atcaaaaaca
    16921 ggtcgtctga aaacctgatt ttctgttttc agagtgcgaa gcctgcccgt gggtacgacc
    16981 ttttcgattt atttgcccaa tgcgcccaat acttcggctt tcacggcttc taccggctga
    17041 gtgccgtcaa ctttgatgta tttaggcgcg tgttcgcctt ccagtttgct gtaaaaatcg
    17101 accaaaactt cggtttgctc gtggtaaacg gcaaggcgtt ttttcacggt ttcttctttg
    17161 tcgtcgtcgc gctgaatcaa atcttcgccg gttacgtcgt ctttgccttc aactttgggc
    17221 gggttgtagg taacgtggta agtacggccg gaagccaaat gcacgcggcg gccgctcata
    17281 cggtcgacaa tcacgctgtc aggcacgtcg atttcaacga ctgcatccaa atccacgcct
    17341 gcttcaacca tcgcttcggc ttgtgccaat gtgcgcggga aaccgtcgaa caggaaaccg
    17401 tttttgcagt cgtcttgcgc gatgcgttct ttgaccatgc cgataatgat gtcgtcgcgc
    17461 accaagccgc cttcgtcaat gatttttttc gcttccaaac ccaagggcgt gccagcctta
    17521 atcgcggcgc ggagcatgtc gccggtagag atttgcggaa tgccgaacgc ggcggtgatg
    17581 aattgcgcct gagtgccttt gcccgcgccc ggcgcgccta aaagtaatgc tttcatgaag
    17641 tgatcctttg aaatgtgttt attggaatca aacagatggt ttacgattct acacggcttg
    17701 gcacaatagt tcaaaaacca actgcacaaa ctgccatttg caagcaaatg ttttgcatac
    17761 ctttgccgag tctgatattc acaagccggc aactttttgg gacaatcccc aaaatttcaa
    17821 aacatcacac cctaccaaaa aggaacatcc gtgaaacgaa tttttctgtt tttggctacc
    17881 aatatcgctg ttttggtcgt aatcaacatt gttttggcgg ttttgggcat caacagccgg
    17941 ggcggcacgg gcagcctgtt ggcgtattcc gccgtcgtcg gcttcactgg ttcgattatt
    18001 tcgctgctga tgtccaaatt catcgccaaa caatcggtcg gcgcggaagt tatcgacacg
    18061 ccgcgcaccg aagaagaagc ctggcttttg aacactgtcg aagcccaagc gcggcaatgg
    18121 aacctgaaaa cgcccgaagt cgccatctac cactcccccg aacccaatgc ctttgccacg
    18181 ggcgcatcga gaaacagctc cctgatcgcc gtcagcaccg gtttgctcga ccatatgacg
    18241 cgcgacgaag tggaagccgt attggcgcac gaaatggcac acgtcggcaa cggcgatatg
    18301 gttacgctga cgctgattca aggcgtggtc aataccttcg tcgtgttcct gtcgcgcatt
    18361 attgccaacc tgattgcccg aaacaacgac ggcagccagt cccagggaac ttatttcctg
    18421 gtcagcatgg tattccaaat cctattcggc ttccttgcca gcttaattgt catgtggttc
    18481 agccgacaac gcgaataccg cgccgacgcg ggcgcggcaa aactggtcgg cgcaccgaaa
    18541 atgatttccg ccctgcaaag gcttaaaggc aacccggtcg atttgcccga agaaatgaac
    18601 gcaatgggca tcgccggaga tacgcgcgac tccctgctca gcacccaccc ttcgctggac
    18661 aaccgaatcg cccgcctcaa atcgctctga gccgatttga aacaacaaaa aaaccgtacg
    18721 cgcaagcagt acggtttttt gtatatgctg tctgaaaaca tcaggtttcc ataccaacac
    18781 caaccgtatc catttccgca ccggatacgc cgcattgata taccctgata aaccgtgtcc
    18841 ggaca
//
LOCUS       DAWKST010000022        18430 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.022, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000022 DAWKST010000000
VERSION     DAWKST010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18430
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.022"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            147..1544
                     /gene="aspA"
                     /locus_tag="WJL87_000529"
     CDS             147..1544
                     /gene="aspA"
                     /locus_tag="WJL87_000529"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ7798999.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFTAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1616..2491)
                     /locus_tag="WJL87_000530"
     CDS             complement(1616..2491)
                     /locus_tag="WJL87_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ7799000.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDIGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVEAAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGKLIGLCLAFAALFCLLWKHGGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFSGNLLVAFVLAGVLMFACLFAKSVRWRVESVVGGILLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWAEVKALFGI"
     gene            complement(2493..3215)
                     /locus_tag="WJL87_000531"
     CDS             complement(2493..3215)
                     /locus_tag="WJL87_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEZ7799001.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPQAEE
                     KFKEIQRAYDTLSDLSKRTQYDASFRGHEERGRQEEAFRREQARREQFYREQMRREQA
                     LRQAFERQASRSRHAYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNGGRAVAPEKWI"
     gene            complement(3237..3716)
                     /gene="ybaK"
                     /locus_tag="WJL87_000532"
     CDS             complement(3237..3716)
                     /gene="ybaK"
                     /locus_tag="WJL87_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ7799002.1"
                     /translation="MSKQTYPVTPAVRVLRENGIKFEPFIYPYEEHGGTAQFAHLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3792..4154
                     /locus_tag="WJL87_000533"
     CDS             3792..4154
                     /locus_tag="WJL87_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ7799003.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKKDAATE"
     gene            4189..5052
                     /locus_tag="WJL87_000534"
     CDS             4189..5052
                     /locus_tag="WJL87_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ7799004.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGDDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     AASAEPLAAQEPCLAFTAECGSDGICSVVLPHRGILHIAPETALPPYDAAAENAWRLH
                     EILSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEETGIVLDSVKDSESFTALAVIKFSAAQKELTVPNGGIFKA
                     VHPFFKTENAE"
     gene            5150..6109
                     /gene="ttcA"
                     /locus_tag="WJL87_000535"
     CDS             5150..6109
                     /gene="ttcA"
                     /locus_tag="WJL87_000535"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ7799005.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPDDKIMVCLSG
                     GKDSYALLDILRQLQASAPIDFELVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATMFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQTLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIAPQKAERKVINILANKPKTCGA"
     gene            complement(6203..9142)
                     /locus_tag="WJL87_000536"
     CDS             complement(6203..9142)
                     /locus_tag="WJL87_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HEZ7799006.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRTADENPELLFAA
                     KQYANNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFV
                     IVDEPHKFPTRESAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSGGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRVEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFAE
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGIRTAVNEAYINNGLMLSRRIDSFEDEDFIR
                     FNTMTYRELLEKLAQTAKIRMQTLHQAFYRVRDELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTDKDGKPRAVKKADLGRFEDTEHRPAAGYLFDEIF
                     YDSDIEHENVANNQIEGVTVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGH"
     gene            complement(9155..10846)
                     /locus_tag="WJL87_000537"
     CDS             complement(9155..10846)
                     /locus_tag="WJL87_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ7799007.1"
                     /translation="MNWLGKSYARLLRNLPPETLISEDKTHNAKPENAGSQNLLIRGD
                     NLEVLKHLKNAYTNSVKMIYIDPPYNTGSDGFVYQDDRKFTPAELACLANIDEDEAAR
                     ILDFTDKGSNSHSAWLTFMYPRLYIARELLKDDGVIFISIDDNEAAQLKLLCDEVFGE
                     GNFIKDLIVNTSEGGGNAKYVVNGHETVLVYAKDITHFDNLKRPKDIRGKKIVINDEL
                     YWIQEDAVREQFGKYGNLHYEDIIEIKGIEFKNKIDKDIENNEYILIPKSYGKTIIGK
                     LRKVSDDFSKFHSILNIGNISKNLTKDGIRELEELFETGKGYSPFETPKPLDLLKRLV
                     LSATFKGNQTDLILDFFAGSGTTAHAVMQLNAEGQNGNRRYICVQLPEKTAEKSEARK
                     AGYPTIFDITKARIEKAAAKIRVEHPDYTGDSGFKIFQTADNFRQHPDKDFSPEQPDL
                     PLNDELSEEQLQTLLTTWTLYDGAALTTPVEPVRLGAYTAYLCEKRLYLMNAGFTSAD
                     LLAFIRKLDDDAAFNPNRVIVFGSNMASAMQHELDQAVRGYANKKEIELNVVIRV"
     gene            complement(11200..11757)
                     /locus_tag="WJL87_000538"
     CDS             complement(11200..11757)
                     /locus_tag="WJL87_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7799008.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDDTVFHRVIDGFVIQGGGFTEDLVQKATDKAVANESGNGLKN
                     TAGTIAMARTANPDSATSQFFINLADNDSLNYKNGQYGYTVFGRVESGMNTVSKIARV
                     KTATHGLYQNVPVQPVKIRRIVVGQ"
     gene            complement(11822..12748)
                     /locus_tag="WJL87_000539"
     CDS             complement(11822..12748)
                     /locus_tag="WJL87_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ7799009.1"
                     /translation="MSEMKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVNPRQFINPDYAQQALYKDQIGICDVLVASKTD
                     LCTPEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIRWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            complement(12736..13212)
                     /locus_tag="WJL87_000540"
     CDS             complement(12736..13212)
                     /locus_tag="WJL87_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ7799010.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            complement(13283..13738)
                     /locus_tag="WJL87_000541"
     CDS             complement(13283..13738)
                     /locus_tag="WJL87_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ7799011.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            complement(13863..14978)
                     /locus_tag="WJL87_000542"
     CDS             complement(13863..14978)
                     /locus_tag="WJL87_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ7799012.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNALP
                     ACGQSLLNLASVSTENRYKIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            complement(15092..16189)
                     /locus_tag="WJL87_000543"
     CDS             complement(15092..16189)
                     /locus_tag="WJL87_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ7799013.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEGLFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQILFGFNATDGAFEVVPQVFSWQLSFTDNIGVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            complement(16271..17854)
                     /locus_tag="WJL87_000544"
     CDS             complement(16271..17854)
                     /locus_tag="WJL87_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ7799014.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTVDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     KHGIAYISYNVYPGWKRQEQLREIMYFAGRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLGGGGKLPAIQKILNHNTYYVAHEYMEIFNDPIYVNGFIEWANRHRLAYIGDTDLH
                     ASFVSWMAEHTRERILALAGGDYIAKEFYSDILSDRQFRRSLLCREEVGDTVRRDESV
                     AVEVIESLNFRPARGETINFDENDTLLSGIRDVMKTGEAFKTEDVAENLARRFPGLTF
                     DRMKINSQLLLQTILGRFSVSSDNAGKQFFEDHKTYVPARFTDYVAAFVEHGAEAFVQ
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            complement(18002..18214)
                     /locus_tag="WJL87_000545"
     CDS             complement(18002..18214)
                     /locus_tag="WJL87_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEZ7799015.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
BASE COUNT         4363 a         4858 c         4437 g         4772 t
ORIGIN      
        1 ataacacccg aaaacaacgt ttcccgtttt cagacggcat acgatatgga caatttcatc
       61 cggccggtat agtgttcgtc ctgcggcggc atctttccga tggcgcattt tttcaatatt
      121 aattatccga cttacaaagg taaaacatga ctgtccgtat cgaacacgat ttattgggcg
      181 accgcgagat tcccgccgaa gtgtattggg gcatccacac tctgcgcgcc attgaaaact
      241 ttaaaatctc cacacaaaaa atttccgacg tgccgcagtt tgtccgcagt atggtgatgg
      301 tgaaaaaagc aaccgcgcag gcaaacggcg aattgggtgc agtaaaaccc gaaatcgccg
      361 ccgccattga aaaggcttgc gacgaagttc tgctgaacaa ccgctgcctc gaccaattcc
      421 cctccgacgt gtatcagggc ggtgcgggaa cttcggtcaa tatgaacacc aacgaagtca
      481 tcgccaacct cgcattggaa gccttgggat acgaaaaagg ccgctacgac atcgtcaatc
      541 cgatggatca tgtcaatgcc agccaatcga ccaacgatgc ctatcccacg ggcttccgcc
      601 ttgccgtgta ttacagcatc ggcgaattgc tcgacaaact gaccgtcctg aaaaacgcct
      661 ttaccgccaa agccgaagaa tttaaagacg ttttgaaaat gggtcgcacc cagcttcaag
      721 atgccgtacc gatgacggca ggacaagaat tccaatcttt ccaagtgttg ttggaagaag
      781 aaatcctcaa cctcgaccgc acgcgccaac tgcttttaga agtcaatttg ggcgcaacgg
      841 caatcggcac gggcgtgaac acgcccaaag gctacgccga actggtggtc aaaaaactct
      901 ccgaagtcag cggcttgcct tgcaaactga ccgaaaacct gattgaggcg acctccgact
      961 gcggcgcata tgtgatggta cacggcgcat tgaagcgcac ggcggtcaaa ctctccaaaa
     1021 tctgcaacga cttgcgcctg ctctcttccg gcccgcgtgc cggcttgaaa gaaatcaacc
     1081 tgcccgaatt acaggccggt tcttccatta tgcctgccaa agtcaatccc gtgattcccg
     1141 aagtggtcaa ccaagtctgc ttcaaagtca tcggcaacga cacgacgatt accttcgccg
     1201 ccgaagccgg gcaactgcaa ttaaacgtga tggagccggt catcgcccaa tgtatgtttg
     1261 aaaccatttc cctcttgggc aatgccgcag tcaacctgtc cgacaaatgc gtcaaaggca
     1321 ttacggtcaa ccgcgaaatc tgcgaacgtt acgttttcaa ctccatcggt ttggtgactt
     1381 atctgaatcc gtatatcgga caccacaacg gcgatttggt cggcaaaatc tgcgcccaaa
     1441 ccggcaaagg cgtgcgcgag gttgtcttgg aacgcgggct gttgagcgaa gaagaactca
     1501 accgcatcct ctctcccgag aacctgatga acccccatct gtaacgttgc agacaaatgc
     1561 cgtctgaagc agggatgctg cttcagacgg cattttgtta tatcaatcag ccggattata
     1621 tgccgaacag tgccttgacc tccgcccaat aaaacaggca ggcgatggaa cacagtgcca
     1681 acacgattcc cgccgtattg attgtgttga ttttttcctt aaaaaacagt gcgcccgaaa
     1741 gcgtccccaa aacaatcacg ccgatattca taccggcaaa aaccagcgtg ggattgtcct
     1801 tcatcatttg gtgcgcggtg atgtaggtta cgatgttcat gaagttcaaa ccgcccaaga
     1861 gtatgccgcc gaccacactc tcaacgcgcc atctgaccga tttggcaaac aggcaggcaa
     1921 acatcagcac acctgccagc acaaatgcaa ccagcaggtt gcctgaaaat gccgtgccgc
     1981 ttttggcaag ctgtttgaac aggatgtcga taatgccgta acccgcccac acgcccagca
     2041 gcaatgccgc ctgccgccac gcgctgcctg attttttgcc gccgccgtgt ttccaaagca
     2101 ggcagaacag cgcggcaaat gcgaggcaca gcccgattag tttgccttcg ctgagttttt
     2161 cgccaaacag cgtcaaggcg gcaacaatcg gcaaaaacag cgacaaacgc tgcgccgcgt
     2221 cggatttgac gataccggcg gcttccacag atttgcccat tatcacgaat acggacggca
     2281 gcagcacgcc caaagcggca aacagcggcc acgtcggcaa aaatgcgccg atattgccga
     2341 tatccggctt caataccagc agggtcagta tgaccgcgac cacataattg acggcgaccg
     2401 cctgcgcgat gtcgattttt ttcttccttg ccattttcag caaaatggaa accgacacgc
     2461 tgaacacgat gctgattaac aggtaagcca tactatatcc atttctcggg tgcaaccgcc
     2521 ctgccgccgt tgaagcggat aaagccggcg atgatgcggt agaaatacca gacgttcgtt
     2581 gcaataataa tcagcacgcc gatacccaaa agggcagtca gcgcacccaa aatataaagc
     2641 caaaatgtgc gccaaaaggt tttaatcagg tattcggtat gtgcggcata gacaatgctg
     2701 tccaaactgt tccgtttcat ataggcgaga atcacgccga ctatgggcat ataggcgaaa
     2761 atcacgccga ctatgggcat gaacagcatg attgcaccca aaccgaacag gatgtaggcg
     2821 gcgaggacat agttgcgccc gcttccgccg ccggacggtt cgtaagcatg gcgcgaacgt
     2881 gaggcctgcc gttcaaacgc ctgtcttaac gcctgttcgc ggcgcatctg ttcgcggtaa
     2941 aactgctccc tgcgcgcctg ttcacggcgg aatgcctctt cctgccgccc gcgttcctca
     3001 tgccctctga aagacgcgtc gtattgcgtc cgtttcgaca ggtcggaaag cgtatcgtaa
     3061 gcccgttgga tttctttgaa cttttcttcc gcctgcggat tacccggatt gcggtcggga
     3121 tggtatttca tcgccagctt gcggtaggcg cgtttgattt cgtccgcccc cgcctgcggc
     3181 gacacgccca atacggcata caaatctttg tccatattgc tccttatact tccccgtcaa
     3241 accgctgcct gtattgtttt cgggttcaaa atatttaaat ctccgggacg gatgccgata
     3301 atgaacccgc gttttccgcc gttgatatag atggtttcca aatccatcac cgactgttcg
     3361 acgtaaatat ccaattttgt ccggatgccg aacggcgttg tgccgccgac cagatagccc
     3421 gtccacttgt ttgcctgtgc gggcgtggcg ggttcgatgt gtttcgcacc caaatgccgc
     3481 gccagattgc gggttgaaat ctgtttgtcg ccgtgcatca agacaatcag ccccttaccg
     3541 ttttcatctt gcaaaacaat ggttttaata accaagtgtt cgtctttgcc gaacaggtgc
     3601 gcaaattggg ccgtaccgcc gtgttcctcg tagggataga tgaaaggttc gaacttgatg
     3661 ccgttttcac gcaaaacgcg tacggcaggt gttaccggat aggtctgttt actcattttt
     3721 cgtcatgcaa aacatggaaa tcacccaaat gcaatgttac catatatccc cctttattga
     3781 aggaaaccga tatggatatc cgatatttcg gcacaacgcc ccgctattcc gaagccgtcg
     3841 gcgcaaacgg cttgattttc ctctccggca tggttcccga aaacggtgaa acggctgccg
     3901 aacagactgc cgacgtgctt gcccaaatcg accgctggct ggcggaatgc ggctcggaca
     3961 aggcacacgt tttagatgcg gtcatctatc tgcgcgatat gggcgactac gcggaaatga
     4021 acggcgtgtg ggacgcttgg gttgccgccg gcagaacgcc tgcccgcgcc tgcgtggaag
     4081 ctcggctcgc ccgtccggag tggcgcgtcg aaatcaaaat aaccgccgtg aagaaagacg
     4141 cggcaaccga ataaacagat ttcctttata tttcagagta cgcaaatgat gaaaacctta
     4201 ctgccctttt tcggcgtggt gcgcgtcagc ggcgaagacc gccaaacctt cctgcacgga
     4261 cagctttcca acgacatcaa taatcttcag accgggcagg cgtgttatgc gacttacaac
     4321 acgcccaaag gccgcgtcat cgccaatatg attgtcgtca accgtggaga cgatttgctg
     4381 ctgattatgg cgcaagactt gcttgaagca acggtcaaac gtttgcggat gtttgtgttg
     4441 cgtgccaaag ccgtttttga aatccttgaa gattacgccg tcggcgcgga attggcagca
     4501 tccgccgaac cgcttgccgc gcaagaaccc tgccttgcct ttaccgccga atgcggttca
     4561 gacggcatct gttccgtcgt cctgccccat cggggcattt tgcacatcgc ccccgaaacc
     4621 gccctgccgc cttatgatgc cgccgccgaa aacgcgtggc ggctgcacga aatcctcagc
     4681 ggttatccgt ggatatgcgc ggctaccaaa gaaactgccg ttgcccaaat gctcaaccag
     4741 cacatcatcg gcggtgttca cttcaaaaaa ggctgctacc cgggacagga aatcattgcc
     4801 cgtgcccagt atcgggggca ggtcaaacgc ggcttggcgg tattgtcggg aaattcggcc
     4861 gtcgaagcag gaaccctgct gacggcagac ggcgaagaga ccggcatcgt gctcgacagc
     4921 gttaaagatt cggaaagctt taccgccctt gccgtgatta aattctccgc cgcacaaaaa
     4981 gaactgaccg tcccaaacgg cggtatcttc aaagcggttc atcccttctt taaaaccgaa
     5041 aatgcagaat aatccctcta atgccgtctg aacctttttc agacggcatt ttaaaatcgc
     5101 ccctgttttc ggttacaatg ccgcatccct aatttttcaa accaccgcca tgtccaaaaa
     5161 aaccaaacaa gaactcgaaa acaacaaact cagcaaacgc ctgcgccacg ccgtcggcga
     5221 cgcgattaac gatttcaata tgatcgagcc ggacgacaaa atcatggtct gcctctccgg
     5281 cggcaaagac agctatgccc tgttagacat cctgcgccag ctccaagcca gcgcgccgat
     5341 tgattttgaa ctggtcgccg tcaatctcga ccaaaagcag ccgggtttcc ccgaagaagt
     5401 attgccgacc tatctcgaaa gcataggcgt gccgtacaaa atcgtcgaag aagacaccta
     5461 ctccaccgtc aaacgcgtat tagacgaagg caaaacgact tgttcgctgt gcagccgcct
     5521 gcgccgcggc atcctctacc gcaccgcaaa agaattgggc tgcaccaaaa tcgccttagg
     5581 acaccaccgt gacgacatcc ttgccaccat gtttttaaat atgttttacg gtggaaaact
     5641 caaagccatg ccgcccaagc tggtgagcga caacggcgaa cacatcgtca tccgtccgct
     5701 ggcgtatgta aaagaaaaag acttaatcaa atacgccgaa ttaaaacaat tccccatcat
     5761 cccgtgcaac ctctgcggct cgcaacccaa cctgcaacgc caagtcatcg gcgatatgct
     5821 gcgcgattgg gacaaacgct ttcccggccg catcgaatcg atgttctccg ccctgcaaaa
     5881 cgtcgtccct tcccaccttg ccgataccga actcttcgac tttgccggat tggaacgcgg
     5941 tcaaaccctg aaacacggcg gcgatttggc gtttgacagc gaaaaaatgc ccgaacgttt
     6001 ttcagacggc agcgaagaag acgaaagcga aatcaaaatc gcgccgcaaa aagccgaacg
     6061 caaagtcatc aacattctgg cgaacaagcc gaaaacctgc ggcgcgtaac cccccgccgt
     6121 caaaaatgcc gtctgaagcc cgtttggcgt ttcagacggc attttaaaat cccgaagcag
     6181 acggcttcca aacatttgtt tctcagtgtc cgttttcaga atgcccgcct gcgggcatat
     6241 tttgcccgat cagttcggct atcctctcgc cgtcaaactg cgttttgaac accaccttga
     6301 tttctttgga aatctcggca aacagctttt cggcatgttt gattttccgc tcttcgcttt
     6361 ttcgcaaatc ttccgccccg tcagtccctt tggcttcaat cacaaagttc agaatctcgc
     6421 cgcttttggt tttcacgata taggcaaaat cgggcgaata cgtgccgccg ccggcaacgg
     6481 ggattttgat ggagtttctc ggtattttgg tgaatacggt tacgccttca atttggttgt
     6541 tggcgacatt ttcatgttct atatccgaat cgtagaaaat ttcgtcgaag agatagccgg
     6601 cggcaggccg gtgctccgta tcttcaaatc tgcccaaatc tgcttttttc accgcgcgcg
     6661 gtttgccgtc tttatcggta aatttggtcg gatggatttt gctgccgaca agccggtaat
     6721 ccagttcaaa tttatggaag gaatgatgaa gcaaaaaccg gttgaagccg tttttgattt
     6781 gggcgatggt ctgcatattc aaaaaatcgc caatgttcag ttcgtcgcgg acgcgataaa
     6841 acgcctgatg caaagtctgc atgcggattt ttgccgtttg tgccagtttt tccagaagct
     6901 cacggtaagt cattgtgtta aaacggataa aatcttcatc ttcaaaactg tctatgcggc
     6961 gggaaagcat aagcccgttg ttgatataag cttcgtttac cgccgtgcgt atgcctgcct
     7021 gcgggaattt ggcggcgttt tcacgcaaat aggcggtaaa taaatcgaca aattcggctt
     7081 catccttgat tttgtactgc aaaacggctt tatggtgaat cagctcccac aaggctttga
     7141 gttcttcata tttgccttcg cgcatgatga tggtgtcttt gccctcgtct ttggcgttgc
     7201 tgactttgcc tttgtccaaa cctttgggga aggcttcggg ataggcggct tttaatttgt
     7261 catagccgtc ttcggcaaag ttttcattgt cgtcaatgat gccgtctgaa aacagttggt
     7321 ttaccaatac cagcggtttg atatcggggt atttttgcaa tattttttgt ttcagctctt
     7381 cggtaaactt tttggagatt tcttcctgaa aagaattgtc gttgatttcg ccgacaagct
     7441 gcttcacaaa gtctttttcg ctgctatcga caaaataatt cagtttgtac ggtacatcgc
     7501 gcacccgcgc catcagctcg tttaccggca ggcgcaggcc gcgtccgact tcttgcagct
     7561 tggaagtcgt gctgccgctg gaacgcagtt tgcaaatctg gaaaacgttg ggattgtccc
     7621 agccttcgcg cagcgtccat ttggaaaaaa taaagcggcg cgggttgtcc aaagacagca
     7681 gtttttcctt atcgtgcagg atttcattga tttcctgctc gattctatcg tcgctgtctg
     7741 tattgtcttt ggaaaaatag ccgccgtggc aggcggatac atcgtccaac gtcttttgca
     7801 ggtaatcgcg gtaaaacggg tcgctttccg ttttcagacg gcgtgccgct tccacgcgaa
     7861 tccagctttc aaatttatct ttcaggctgc ctgaaagctc gttgccgctg cggtagcccg
     7921 cgatatcgtc aataaaaaac agcgtcagcg gcttgatttt gggctgtggc gcgcgttctg
     7981 ccaaaagcgc gcgttccagc ttgaaatgtt cggcaaccgc ccgctgcatc atcgcatcct
     8041 gcaccgtttg cgaataggaa taagggttga tgacggcacc cgttttcaac tccaagccgt
     8101 tgcttaacac caccacggtt ttattcattt tgtcgatttt caaatccgaa atagccggat
     8161 ggatttgcgc caaatcttcg ccttttgcca gtttgaacgt ctgctttttg tccttttcgt
     8221 ttaattcaaa tttcgcttct ttgccgcccg acgacaccag ttttaccgcc gcatccatgc
     8281 cgccctgcat ttcttcctga aacacgcgca cgcctttgac cagcccgtcg ttaaacgcgt
     8341 ctactgccgt caaacggtaa agcaagttgt aatattcatc gttaaatgtc gcaccgtagc
     8401 gcaaaatata ttgcggtttt aagcgtttga tattgcccca cgttttcgcg ctctcccggg
     8461 tcgggaattt atgcggttcg tccacaatca caaacgggcg cacggcggcc aatgcatcaa
     8521 cgggattgtc aaacaaatct ttcaatgcct tgtcgcccgt atcgttcatg gacgacgaat
     8581 taaccatgcc cgcgttaatc agcagcacat ggatttcctt tttgttttcc gctttgacaa
     8641 attgctcaat cgttatgggc gcattggact ttttgccctt attctttttc gcgctttcca
     8701 ccacataggt tttcaggcgc acgccttcat aatcgccgcc gaaatcctgt tcaaaatgct
     8761 ctgccaaagc cttgctttgc aaaaactgct gtgttcccgc cttaatggac aaagtcggca
     8821 cgaccacgat aaatttgaac acgcccagcc aacggtgcag ctcgaacatg gtttgtgtgt
     8881 aggtataggt tttgcccgtg cccgtttcca tggaaatatc aaggatattt tggtcgtccg
     8941 aacggtcggg gaatcggccg tctataccgt tttggctttg cactttcagg atattgtttg
     9001 cgtattgttt tgcagcaaac aaaagttcgg gattttcgtc tgccgtccga tatttgggcg
     9061 ttgccccgtc aaacacgccc aaaaccgccg aaaccgcccg catttggtgc ggctggtttt
     9121 tctcgtaatt aaaaccgctc atgaattgcc tccgtcaaac cctgataacc acattcaact
     9181 caatctcttt tttattggca taaccgcgaa ccgcctggtc aagttcgtgc tgcatggcgc
     9241 ttgccatatt gctgccgaat acaatcacgc ggttgggatt gaaagccgca tcgtcgtcca
     9301 gcttgcggat aaacgccaac aaatcggcag aagtaaaacc ggcattcatc agatacagcc
     9361 gtttttcgca cagatacgcc gtgtaagccc ctaaccgcac aggctcaacc ggcgtggtca
     9421 gtgccgcccc gtcatacagc gtccaggtgg tcagaagcgt ttgcagctgt tcttcgctta
     9481 attcatcgtt aagcggcaaa tccggttgtt cgggcgaaaa atccttgtcc ggatgctgcc
     9541 tgaaattgtc tgccgtttga aagattttga agcccgaatc gcccgtgtaa tcgggatgtt
     9601 cgacgcggat tttggcggcg gctttttcta tgcgggcttt ggtgatgtcg aagatggtcg
     9661 ggtagcctgc tttacgggct tcggattttt cagcggtttt ttcgggaagc tgtacacaga
     9721 tatagcggcg gttaccgttt tgtccttcgg cgttaagctg catcacggcg tgggcggttg
     9781 tgccgctgcc tgcgaagaag tctaggatta ggtcagtttg atttcccttg aaagttgcag
     9841 aaagaactaa tcttttcaat aaatctaatg gttttggtgt ttcaaaagga gaataacctt
     9901 ttccagtttc aaacaactct tctaattcac gaataccatc tttagtaaga tttttactta
     9961 tatttccaat atttaagatg ctatgaaatt tggaaaaatc atcggatact tttctaagtt
    10021 ttccaataat tgttttccca taagacttcg gaattaagat atattcatta ttttcaatat
    10081 ctttatcaat tttgttttta aactcaattc cttttatttc aataatatct tcgtaatgca
    10141 agttcccata tttgccaaac tgttcgcgaa cggcatcttc ctgtatccaa tataattcat
    10201 cattgattac aatttttttg ccgcgtatat cttttggtct ttttagattg tcaaaatgag
    10261 taatatcttt cgcataaact aaaacagttt catgcccatt tacaacatat tttgcattac
    10321 caccaccttc tgatgtatta acaattaaat ctttaataaa attcccttcc ccaaacactt
    10381 catcacacaa caacttcaac tgcgccgctt cgttatcgtc aatcgagata aaaatcacgc
    10441 cgtcgtcctt taacagttcg cgggcgatat acaggcgcgg atacataaag gtcaaccatg
    10501 cactgtgcga gtttgagcct ttgtcggtga aatctaaaat ccgcgcggct tcgtcttcgt
    10561 caatattggc taagcaggca agttcggctg gtgtgaattt gcggtcgtcc tgatagacaa
    10621 agccgtctga tccggtgttg tagggcgggt cgatgtaaat catcttcacg ctgtttgtgt
    10681 aggcgttttt taagtgtttc aacacttcta gattgtcgcc acgaatcagt aggttttggc
    10741 tgcctgcatt ttcgggtttg gcgttgtgcg tcttgtcttc acttatcagg gtttcgggcg
    10801 gcaggttgcg aagcaggcga gcatatgatt tgcccagcca gttcatttcg taaaattcgc
    10861 gtccgatgtc ggtctgtggc gcaatttcgg cttgtaatct gtcgataagg aaatttccgt
    10921 ctgcgtcaaa acaggcggga aacagttttt tgagctgttc gagttgggta gagttggcgg
    10981 taatgccgtc tgaagtgtag attgcctcgg tgttcacccc ggctgtgtcg attagatcag
    11041 gggcttgggt tgggttgggt tgggttgggt tgggttggca gcattttaaa atcctcggtt
    11101 tgaatttgtc aatatcaact atctgtttta aaatattttt tcacttcaga cggcgttttt
    11161 tgtgaaacgg gcgacgccgt ctgaacatct gtctgcgtgt tactgtccga caacaatgcg
    11221 acggattttg acgggctgta cgggtacgtt ttgataaagg ccgtgcgtgg cggttttgac
    11281 gcgggcgatt ttggaaacgg tgttcatgcc gctttcgacc ctgccgaaaa cggtatagcc
    11341 gtattgtccg tttttgtagt tgagtgaatc attgtccgcc agattgataa agaattggct
    11401 ggtggcggaa tccggattgg ccgtccgcgc catggcgatg gtaccggcag tgtttttcaa
    11461 gccgttaccg gattcgttgg caacggcctt atcggttgcc ttttgtacca agtcttcggt
    11521 aaatccgccg ccctggataa cgaaaccgtc gataacgcgg tgaaaaacgg tgtcgtcgta
    11581 aaagcctttt cgggcatagc gcacgaaatt agcaacggtc ttgggggctt tggactcgtc
    11641 caaaaccaaa cggatattgc ccatatcggt ttccatcaaa acatgggttg ccgccataga
    11701 cggcagggaa accgccaaaa gcagcgcggt taaaacggtt ttgaatttgg gtttcatccc
    11761 gtcctcctca gaccttcaga cggcattttc atttcatatg ccgtctgaag gcgcgtgaac
    11821 gctattccaa tgcgtctttg agcttttgtt cgattaaatc cgcatcaaac gatttggcaa
    11881 tcaattcaaa acgcgaatcg cgccgccatg agacttggtt cgcgccccac tgtccgtcta
    11941 cccagttgag ccacacccac gttcccaata cttgaaacac gcctttggcg cggacgagtc
    12001 cttcggtcat tttgggcagg tcgttgaaaa agttggtcag cttttcgccg tcgaaatcgc
    12061 gtccggcggg gaatgtgaaa ccttgcgact ggaagcccat ggtattatcc ggcagggctt
    12121 tgaggcggta gcgtgatttt tcgatgacgg gaatatcgag ccaccggatg tcgagttgtg
    12181 cgttttggac ttcaaccact ttggctttgg gcgggaacag ttttgccgct ttgtcgtgaa
    12241 attcggcaag ctgttcgggg gtgcataaat cggttttgct ggcgaccaat acgtcgcaga
    12301 tgccgatttg gtctttatac aacgcctgtt gcgcgtaatc ggggttgatg aactggcgcg
    12361 gattgacgac ggtaaagacg gcgccgattt ccaaaaggct gtccagcggt ttggctttga
    12421 gttcgtcgat gacgctggct gcgtgagcca gtccgcttgc ttcaatcatc aggcggtcgg
    12481 gcttggcgtc gcgcagcatt ttctgcacgg ttacgcccat ttgcgggccg gcggtgcaac
    12541 acaaacagcc gccggcgatt tctgccacag ggataccgtt gtcgctcaat accgcgccgt
    12601 caatgccgat ttcgccgaac tcgttgacga tgatgaccca tttttcgttc gggtcttttt
    12661 gcgccatcag gcttttgagc gcagtggttt tgcctgttcc cagaaaacct gaaatcaggt
    12721 ggactttggt ttttttcatt tctgacattt tttacagatt ccagttaaaa caacgtgttc
    12781 ttctttcagc gcaaagccgc tttcggcaac gcctgcgcgc agtgccgccc actcgtggct
    12841 gagggtttgc tcgtccgccg tgccgcattc ggtgcagacc aaaataaacg cgctgtggtg
    12901 cgcttcggct tcttcgtggt cgtggcaatg gtcgtcgcac tcgtgctgcg cgtggctgca
    12961 caaaatatag ccgttgaccg ccgccacttt gtgcaaaacg ccctgctccg cccaaaaatc
    13021 aagggcgcgg taggcggtag gcggtgcaag cacgccctcg ctttgctgct gcatctgcga
    13081 caagacgttg taggctttaa tcacgccgct ttgctgcaag acaatatcta atacctgctc
    13141 gcgcaaagcg gttacctgca agccttcgct gcgtgcctgt tcgataattt tctgtttgaa
    13201 atttgttttc ataaattctc tgtttatgcc gtctgaacaa ccgatacggc aggaggcggt
    13261 tttatatttg tattcaattg ctttatttgg aaatcttttc caacaatgcc cgacagcccg
    13321 catccgcaag cctgaaggtt tcttcaaaat cgcccgtata ccacggatcg gggacatggt
    13381 cgtaaccgct ttcgggtatc aggtcggtca gcttgaatat tttttccggc cgcctgccga
    13441 aggttctttc caattcggac aaattcttgc cgtccatcgc gatgatgcag tcaaacgccg
    13501 ccgcatcgct ttggcggatt ttgcggctgg taaagcctga agcatcgata ccgtgttttt
    13561 tcaatatctt tgccgtctcg cggtgcatat cttcgccgtc gtgccagccc gatgtccccg
    13621 cgctgtccgc ttcaagggga atgcccgctt cggcggcgcg gcggcgcaaa atgtattccg
    13681 ccatcggcga acggcagatg ttgccgaggc agacaaaaag gattttcggt tttttcatat
    13741 cccctccctg ttccggcgcg atgccgtctg aagcggaaac cctttcagac gtcatcggtc
    13801 ggttaatcgt aaccgcataa ggcagaacca gccaaaacat ccatctggaa gaaatggttt
    13861 ggctaaatct taggcatatt taataagtgt ccaatattag aagccgtatg ctccaaatag
    13921 agactggcat ttttcaaact atcttctaaa ggttcacttt tctccaaaat agaaaaggca
    13981 gcttggatat tttcaaatgg cagggaaggc aaatcttcaa cgagactgcc acaaatagcg
    14041 acaacaggaa ctccgacagg ggttcttttt gctacaccaa taggcgcttt ccctgctaaa
    14101 ctttgacgat ctagtcttcc ttcaccaacg ataaccaagt caacatctga cactttctta
    14161 tcaaagtcaa tcaagtccag gcaggtgtca attccagata cgatacttgc ctgagcaaag
    14221 gcgcacaaac caccagcgat gcctccacca gctcctgctc ctttaagttt taatgttgca
    14281 ggggagactt tttcataaaa atcttgtatt gcctgatcta cggcctcaaa catagtagaa
    14341 tccaaccctt tttgcttgcc aaacgtatag gtcgcacctt ggtgtccaca taagggactc
    14401 acaacatctg ctaaaatacg aatgtgaaca tcttcaggaa ttttatagcg attttctgtt
    14461 gaaacagaag ctaggtttag taaggattga ccgcaagcgg gtaaggcatt tccatcctca
    14521 tcataaaatt gataacctaa accagcagca atcccaatac ctccatcatt actggccgta
    14581 ccgccaacgc caatatagat ttctttaatt tcttgactaa tgaggtggcg aatcaattct
    14641 ccaataccac gagtttggat ttgtaatgga tttcgtttct ctagcgggat ttttccaaga
    14701 ccaaccaaat cagcaacttc gaatagggct agttgttctt tttgaaaata gcgcatgact
    14761 tctttttgtc caaaaggtcc tgtcacttgg agacattttt cttctaggtc aagagaatgt
    14821 cggatagcat ctacagtgcc ttctccccca tcaccgacag gacagaggag acattccaca
    14881 tctgctatcg attgttggaa gcctcttttt attgcttcag ctacctgttg agctgtcaag
    14941 ctttccttaa acgaatccgg tgcaattaca atcttcatat ttataattca tcctttcgtt
    15001 tcactcaagg cacaacacag aatgaaaaag tgttgtgctc tttattttga tttattatag
    15061 aaatgagaaa gcctatcact actacaaatc actatgcgct gaaaaacgga ttgtgccctt
    15121 cccgtttcaa tgcttccgca tagctcggga tgctttcctg ttcgcccaag ggattgtgca
    15181 acaggtaaac gtgttctaca cggcattctg ccattaaatc caaaaaattt tgctgaataa
    15241 cgccaatatt gtcggtaaag gaaagctgcc acgaaaacac ctgcggcacg acttcaaacg
    15301 cgccgtcggt cgcattaaac ccgaataaaa tctgtccgcc cgcctttgcc gctgcgacca
    15361 cgccgacgcg cggactctgc atacggacgg cgcgtatcgc gtttgccgca aacacatcca
    15421 tcgccatgcg ctggcgcgtg tggctgccgt cggaaagcgc gcgtaccgga gaatccgggg
    15481 aaagcggatt ggcaaacagc gtaacgcctt ccgatgcaag attttcctgc catacctgca
    15541 tcaagggcag acctgcctga agaaggttct gacccaatgc cgtctgaact ttgccgctgc
    15601 cgaaacccag cgcgtaaacg acgtggacgg actgcccttc gggcaaaagc aaagggcgcg
    15661 gggcgaaacg gcgtaagtgt tgcgccgccg cttcggtatt ttgtttggca cgccaccact
    15721 catccggcgc gaccgcactc aaatcggagg aatgtacaag ataaggcagc cattgcgtct
    15781 cttgcgtcaa cgcgcgcagg tgcggatagt tttgcaaaca ggcaaacaat ccctccgtcg
    15841 gcagcttcat ctcgattgcc cgttctttct tgcagccgga caccagcacg accggcaggg
    15901 cgaaccactg cacttcgcct tctttctcgc aatcgagtac cgcgttcaca ctggaaagca
    15961 gcgcggcata agttccggca tcgggcgaca tcgtcagcgc gagggaaagg ttgatatagt
    16021 ggttttgctc aagcatcccc ctgatttcgg tttgaagttg gccggacgag agtttgcgcg
    16081 aagcctggga agaattatgc gccaactggt aggcattgag cagcaggtgg tttttaatcg
    16141 gattttgggg atacgggcgc gtatcgggca aggtaaatgt ctggttcata tatgtcgtgt
    16201 accgcttttc gcagtgtgtg tttttcctgt ttgaaaatat atcggacgga ttgccgtcgg
    16261 gacttgcccg tcaatccgcc gaaacgagaa aatgcctgtc tgccaagtct gccaatattt
    16321 cttccacata cacttcggca ggcggatgga atgtcaaacc gtcgggcgtg gtgctgacga
    16381 tgttcaagtt ttcggcaacc gtttcaatca atgcctgttt gctcgtcggc cgcgacaatt
    16441 gccgcataat gtacaaatgc ccgtacccga atgagggggt gctttcgttg taacggttgg
    16501 caggctggac aaacgcttcc gcgccgtgtt ccacaaaggc ggcgacataa tctgtaaaac
    16561 gcgccggcac ataggtttta tggtcttcaa aaaattgttt gcccgcattg tctgatgaaa
    16621 cagaaaaacg cccgagaatg gtttgcaata aaagctggga attgattttc atacggtcga
    16681 atgtcaaacc gggaaatcgg cgggcgagat tttccgcaac atcttccgtt ttaaacgcct
    16741 ctccggtttt catcacatcg cgtatgcccg aaagcagggt atcgttttca tcaaagttga
    16801 ttgtttcccc tcttgccggg cggaaattca aactttctat cacttcgacg gcaaccgact
    16861 cgtcacgcct gacagtatcc ccgacttcct cacggcataa aagcgaacgg cggaattggc
    16921 ggtcggataa aatatcgctg taaaattctt tggcaatata atcgccccct gccaatgcca
    16981 gaatccgctc ccgcgtatgc tccgccatcc aagaaacaaa agacgcgtgc aaatcggtat
    17041 ccccgatata cgccagccgg tggcggtttg cccattcgat gaagccgtta acgtaaatcg
    17101 ggtcgttgaa tatttccata tattcgtgtg cgacataata agtattgtga ttcaatattt
    17161 tttgaatcgc cggaagtttg ccgccgccgc ccaagccttt gtcgttttcc aaaatttccg
    17221 ccagcgcctt gagcgcgtcc aagcctttcc gcgtccgcgc ttccaagggt tcttcaagca
    17281 catccctgcc ggcaaagtac ataatttcgc gcaactgctc ctgccgtttc cagccagggt
    17341 aaacattgta tgaaatatag gcaatgccgt gtttggtcag gttgttccgg caaatcgaaa
    17401 agattttgtc tttaactgcg tcaggcaccc acgaccaaat gccgtggacg atgatatagt
    17461 cgaacttccc gaatgactca tcgacggtca aaatatcttt ttcttccaga cgcacatttt
    17521 tcaagcccat tttttcaatg atggcgttgc cctgcgcaac ctgcctgccg gacaagtcga
    17581 taccgacaaa ttccgcatcc gggtaataaa gagcctgcgt gatgatgttg ccgcccatcg
    17641 aacagcccag ctccaagacc ttggcattgg cggcgggcgc gggctgcaaa cccatcatgc
    17701 gggcgcgagc ctccaaggcg ttgatggcgg tttgggaaaa tgctttggat tcatacatca
    17761 aatcatcata tgaatttttg atgttggaca cgtccggcac accgttctcc gttgcagcac
    17821 gcgcaaaggc ggcttttttc tttttgttcg gcatattttg tttgtctgaa ggcactattg
    17881 cccgcaagtt taaccaattc atcctacccg ttcaactaaa tcaaatgcca tctgaaggcg
    17941 cggagcgtac ttcagacggc atcggggagg cgcgaaggct tcagacggca tttttgccgc
    18001 tttatttcaa cgcggcatca tagccgccgt cttcaacggc ttcaatcaac gcccctgcat
    18061 cggtttgcgc ggggtcgtat ccgacggtcg cacttttgtt ttcaaggctg acttcgacgc
    18121 ttgccacgcc ttttacgcct tccaatatcc gggtaacgct tttgacgcag ccgccgcagc
    18181 tcatgccgcc gatgtcgagg ataagggttt ccatcatttt cctttcgttg gaactgcaat
    18241 ctgacgggcg ttattgtaag tcggggcgtg aacttgggca aacgcggaaa cggtacggcg
    18301 gtttggaaaa agacggacgc ttgcgcataa tggcggcaat tcccatcagg acgacaacaa
    18361 tgaacgcttc acaaaaaccc tggttgagca tcatcgcctt ggcaatcggc gcatttatag
    18421 tggattaaca
//
LOCUS       DAWKST010000023        18005 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.023, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000023 DAWKST010000000
VERSION     DAWKST010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18005
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.023"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            17..328
                     /gene="rpsJ"
                     /locus_tag="WJL87_000546"
     CDS             17..328
                     /gene="rpsJ"
                     /locus_tag="WJL87_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEZ7799016.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLSAGVDVEIKV
                     Q"
     gene            449..878
                     /locus_tag="WJL87_000547"
                     /pseudo
     CDS             449..878
                     /locus_tag="WJL87_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1332..1976
                     /gene="rplC"
                     /locus_tag="WJL87_000548"
     CDS             1332..1976
                     /gene="rplC"
                     /locus_tag="WJL87_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ7799017.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            1976..2596
                     /gene="rplD"
                     /locus_tag="WJL87_000549"
     CDS             1976..2596
                     /gene="rplD"
                     /locus_tag="WJL87_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ7799018.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            2593..2907
                     /gene="rplW"
                     /locus_tag="WJL87_000550"
     CDS             2593..2907
                     /gene="rplW"
                     /locus_tag="WJL87_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ7799019.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANAAKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            2913..3746
                     /gene="rplB"
                     /locus_tag="WJL87_000551"
     CDS             2913..3746
                     /gene="rplB"
                     /locus_tag="WJL87_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ7799020.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            3753..4031
                     /gene="rpsS"
                     /locus_tag="WJL87_000552"
     CDS             3753..4031
                     /gene="rpsS"
                     /locus_tag="WJL87_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ7799021.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            4040..4369
                     /gene="rplV"
                     /locus_tag="WJL87_000553"
     CDS             4040..4369
                     /gene="rplV"
                     /locus_tag="WJL87_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ7799022.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            4379..5071
                     /gene="rpsC"
                     /locus_tag="WJL87_000554"
     CDS             4379..5071
                     /gene="rpsC"
                     /locus_tag="WJL87_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ7799023.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            5055..5471
                     /gene="rplP"
                     /locus_tag="WJL87_000555"
     CDS             5055..5471
                     /gene="rplP"
                     /locus_tag="WJL87_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ7799024.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            5471..5662
                     /gene="rpmC"
                     /locus_tag="WJL87_000556"
     CDS             5471..5662
                     /gene="rpmC"
                     /locus_tag="WJL87_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ7799025.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            5662..5925
                     /gene="rpsQ"
                     /locus_tag="WJL87_000557"
     CDS             5662..5925
                     /gene="rpsQ"
                     /locus_tag="WJL87_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ7799026.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            6147..6515
                     /gene="rplN"
                     /locus_tag="WJL87_000558"
     CDS             6147..6515
                     /gene="rplN"
                     /locus_tag="WJL87_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ7799027.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            6527..6850
                     /gene="rplX"
                     /locus_tag="WJL87_000559"
     CDS             6527..6850
                     /gene="rplX"
                     /locus_tag="WJL87_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ7799028.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMSLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            6860..7399
                     /gene="rplE"
                     /locus_tag="WJL87_000560"
     CDS             6860..7399
                     /gene="rplE"
                     /locus_tag="WJL87_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ7799029.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            7402..7707
                     /gene="rpsN"
                     /locus_tag="WJL87_000561"
     CDS             7402..7707
                     /gene="rpsN"
                     /locus_tag="WJL87_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ7799030.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            7723..8115
                     /gene="rpsH"
                     /locus_tag="WJL87_000562"
     CDS             7723..8115
                     /gene="rpsH"
                     /locus_tag="WJL87_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ7799031.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            8129..8662
                     /gene="rplF"
                     /locus_tag="WJL87_000563"
     CDS             8129..8662
                     /gene="rplF"
                     /locus_tag="WJL87_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ7799032.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            8676..9029
                     /gene="rplR"
                     /locus_tag="WJL87_000564"
     CDS             8676..9029
                     /gene="rplR"
                     /locus_tag="WJL87_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ7799033.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKFGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            9048..9566
                     /gene="rpsE"
                     /locus_tag="WJL87_000565"
     CDS             9048..9566
                     /gene="rpsE"
                     /locus_tag="WJL87_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ7799034.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            9559..9744
                     /gene="rpmD"
                     /locus_tag="WJL87_000566"
     CDS             9559..9744
                     /gene="rpmD"
                     /locus_tag="WJL87_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ7799035.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            9746..10180
                     /gene="rplO"
                     /locus_tag="WJL87_000567"
     CDS             9746..10180
                     /gene="rplO"
                     /locus_tag="WJL87_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ7799036.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            10192..11502
                     /gene="secY"
                     /locus_tag="WJL87_000568"
     CDS             10192..11502
                     /gene="secY"
                     /locus_tag="WJL87_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ7799037.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            11507..11725
                     /gene="infA"
                     /locus_tag="WJL87_000569"
     CDS             11507..11725
                     /gene="infA"
                     /locus_tag="WJL87_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ7799038.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            11746..11859
                     /gene="rpmJ"
                     /locus_tag="WJL87_000570"
     CDS             11746..11859
                     /gene="rpmJ"
                     /locus_tag="WJL87_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ7799039.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            11925..12287
                     /gene="rpsM"
                     /locus_tag="WJL87_000571"
     CDS             11925..12287
                     /gene="rpsM"
                     /locus_tag="WJL87_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ7799040.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            12307..12702
                     /gene="rpsK"
                     /locus_tag="WJL87_000572"
     CDS             12307..12702
                     /gene="rpsK"
                     /locus_tag="WJL87_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ7799041.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            12722..13342
                     /gene="rpsD"
                     /locus_tag="WJL87_000573"
     CDS             12722..13342
                     /gene="rpsD"
                     /locus_tag="WJL87_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ7799042.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            13368..14354
                     /gene="rpoA"
                     /locus_tag="WJL87_000574"
     CDS             13368..14354
                     /gene="rpoA"
                     /locus_tag="WJL87_000574"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ7799043.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            14378..14746
                     /gene="rplQ"
                     /locus_tag="WJL87_000575"
     CDS             14378..14746
                     /gene="rplQ"
                     /locus_tag="WJL87_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ7799044.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            14893..15606
                     /gene="minC"
                     /locus_tag="WJL87_000576"
     CDS             14893..15606
                     /gene="minC"
                     /locus_tag="WJL87_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ7799045.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPNHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            15634..16449
                     /gene="minD"
                     /locus_tag="WJL87_000577"
     CDS             15634..16449
                     /gene="minD"
                     /locus_tag="WJL87_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ7799046.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            16453..16716
                     /gene="minE"
                     /locus_tag="WJL87_000578"
     CDS             16453..16716
                     /gene="minE"
                     /locus_tag="WJL87_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ7799047.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            16720..17640
                     /gene="oxyR"
                     /locus_tag="WJL87_000579"
     CDS             16720..17640
                     /gene="oxyR"
                     /locus_tag="WJL87_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ7799048.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(17637..17828)
                     /locus_tag="WJL87_000580"
     CDS             complement(17637..17828)
                     /locus_tag="WJL87_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799049.1"
                     /translation="MLIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNI
                     LAYRVVGSGKWRFENNACA"
BASE COUNT         4948 a         3461 c         4460 g         5136 t
ORIGIN      
        1 tgaaggatat tgataaatgg caaaccaaaa aatccgtatc cgcctgaaag cttatgatta
       61 cgccctgatt gaccgttctg cacaagaaat cgttgaaact gcaaaacgta ccggtgccgt
      121 tgtaaaaggc ccgattcctt tgccgaccaa aatcgagcgt ttcaacattt tgcgttctcc
      181 gcacgtgaac aaaacttccc gtgaacaatt ggaaatccgc acccacttgc gcctgatgga
      241 catcgtggat tggaccgata aaactaccga tgcgctgatg aagctggatt tgtcggccgg
      301 tgttgatgta gaaatcaaag tccaataatt cggactattg aaaatcccca agcaatcaat
      361 gcttggggat tttttatgtt atgccgagac ctttgcaaaa ttccccaaaa tctcctaaat
      421 tcccaccaag acatttaggg gatttctcat gagcaccttc tttcaacaaa ccgcacaagc
      481 catgattgcc aaacacatcg accgtttccc gctattgaag ttggatcaag tgattgattg
      541 gcagccgatc gagcaaaggt acgaccatct atgccgataa aggctatgac agtgcggaaa
      601 accggcaaca tctgaaagaa catcagttgc tggacggcat tatgcgcaaa gcctgccgca
      661 accgtccgct gtcggaagcg caaaccaaac gcaaccgata tttgtcgaag ccccgttatg
      721 tggtcgaaca aagcttcggt acgctgcacc gtaaattccg ctatgcgcgg gcagcctatt
      781 tcgggctgct caaagtgagt gcgcaaagcc acctgaaagc gatgtgtttg aacctgttga
      841 aagcggccaa caggctaagt gcgcccgctg ccgcctaaaa ggcagcccgg atgcctgatt
      901 atcgggtatc cagggaagat taagggggta tttgggtaga attaggaggt atttggggcg
      961 aaaacagccg aaaacctgtg ttggggtttc ggctgtcggg agggaaagga attttgcaaa
     1021 ggtcccagca tacgaaaact accatctcaa agaaataatg gatgcagtca tcaaccatat
     1081 tccggtttgt cggagaaaga tgcatacgct gtgatgaccg gataccgacc ctttaaaaga
     1141 gtccgaccct atgccgtctg aaaattcaaa acgcttcaga cggcatattg aagatatttc
     1201 tgatatttct gttgatattt ctttgacttg tcagatataa tgccgagctt ggtacatttg
     1261 tgccaagttt aactttgtct gaaagacagg ccaatcgtag cctgtccctt tactttaaaa
     1321 ggaaaataat catgacttta ggtctggttg gacgcaaagt tggtatgacc cgcgtgttcg
     1381 acgaacaggg tgtttctgtt ccggtaaccg ttttggatat gtctgccaac cgcgttacac
     1441 aagtaaaatc caaagatact gacggctata ctgccgttca agttaccttt ggtcagaaaa
     1501 aagccaatcg tgtcaataaa gccgaagccg ggcactttgc aaaagcaggt gttgaagccg
     1561 gtcgcggttt gattgagttt gctttgactg aagaaaaact ggctgaattg aaagccggtg
     1621 acgaaatcac cgtttctatg tttgaagtcg gccagctggt cgatgtaacc ggtacctcta
     1681 aaggtaaagg tttctccggt acgatcaaac gtcataactt cggtgcccaa cgtacttccc
     1741 acggtaactc ccgctctcac cgcgttccag gctctatcgg tatggcgcaa gacccgggtc
     1801 gcgtgttccc cggtaaacgc atggccggcc aatacggcaa caccaaagca actgttcaaa
     1861 aattggaagt tgtccgtgtt gatgcagaac gccaactgct gttggttaag ggtgctgttc
     1921 caggtgcggt caacagcgat gttgtagttc gtcccagcgt gaaagtaggt gcgtaatgga
     1981 attgaaagta attgacgcta aaggacaagt ttcaggcagt ctgtctgttt ctgatgcttt
     2041 gttcgcccgc gaatacaatg aagcgttggt tcatcagctg gtaaatgcct atttggcaaa
     2101 cgcccgctcc ggcaatcgcg ctcaaaaaac ccgtgccgaa gtaaaacact caaccaaaaa
     2161 accatggcgt caaaaaggta ccggccgtgc ccgttccggt atgacttctt ctccgctgtg
     2221 gcgtaaaggt ggtcgcgcgt tcccgaacaa acccgacgaa aacttcactc aaaaagtaaa
     2281 ccgcaaaatg taccgtgccg gtatggcgac tattctgtcc caattgactc gtgacgagcg
     2341 tttgtttgcg attgaggcgt tgactgccga aactcctaaa accaaagttt ttgccgaaca
     2401 agtgaaaaat ctgggtctgg agcaagtgtt gtttgtaacc aaacagctcg acgagaatgt
     2461 ttacttggct tcacgcaact tgccaaacgt gttggttttg gaagctcaac aagttgatcc
     2521 ttacagcttg ctgcgttaca aaaaagtaat catcactaaa gatgcagttg cacaattaga
     2581 ggagcaatgg gtatgaatca acaacgtttg actcaagtga ttttggcacc tatcgtttct
     2641 gaaaaaagca acgtattggc tgaaaaacgc aaccaaatga cgtttaaagt tttggcaaat
     2701 gcagccaaac ctgaaatcaa agcggctgtc gagctgctgt tcggtgttca agttgcttct
     2761 gtaactaccg ttacaactaa aggcaaaact aagcgttttg gtcgtacttt gggccgccgc
     2821 agcgatgtta aaaaagctta tgtaagcttg gctgccggtc aagagttgga tttggaagcc
     2881 gctgctgcag ctgcagataa ggaataaaca aaatggcaat cgttaaaatg aagccgacct
     2941 ctgcaggccg tcgcggcatg gttcgcgtgg taacagaagg tttgcacaaa ggtgcacctt
     3001 atgcaccctt gcttgaaaag aaaaattcta ctgccggccg taacaataat ggtcatatca
     3061 ccacccgtca caaaggcggc ggtcataaac accattaccg tgttgtagac tttaaacgta
     3121 acaaagacgg cattcctgct aaagtagagc gtatcgaata cgatcctaac cgtactgcct
     3181 tcatcgcact gttgtgctat gcagacggcg agcgtcgtta catcattgct cctcgcggta
     3241 ttcaagccgg tgctgtatta gtttccggcg ctgaagctgc catcaaagta ggcaacaccc
     3301 tgccgatccg caacattccc gttggtacga ctatccactg tatcgaaatg aaacccggta
     3361 aaggtgctca aatcgcacgt tctgccggtg cttctgcggt attgctggct aaagaaggcg
     3421 cgtacgctca agtccgtctg cgctctggcg aagtccgtaa aatcaacgta aattgccgtg
     3481 cgaccatcgg tgaagttggt aacgaagagc aaagcctgaa aaaaatcggt aaagccggtg
     3541 ccaaccgttg gcgcggtatt cgtccgaccg tacgcggtgt tgtcatgaat cctgtcgatc
     3601 acccgcatgg tggtggtgaa ggccgtacgg gcgaggcccg cgaaccggtc agcccatggg
     3661 gtactcctgc taaaggctac cgcactcgta ataacaaacg cacggataac atgattgttc
     3721 gtcgccgtta ctcaaataaa ggttaattta gtatggctcg ttcattgaaa aaaggcccat
     3781 atgtagacct gcatttgctg aaaaaagtag atgctgctcg cgcaagcaac gacaaacgcc
     3841 cgattaaaac ctggtctcgt cgttctacca ttctgcctga ttttatcggt ctgaccattg
     3901 ctgtgcacaa cggccgcacc catgtgcctg tgtttatcag cgacaatatg gttggtcata
     3961 aattaggcga attctcattg acccgtacct ttaaaggcca cttggccgat aaaaaggcta
     4021 aaaagaaata aggtgaatca tgagagtaaa tgcacaacat aaaaatgccc gtatctctgc
     4081 tcaaaaggct cgtttggtag ctgatttgat tcgtggtaaa gacgttgccc aagctttgaa
     4141 tattttggct ttcagtccta aaaaaggtgc cgagctgatt aaaaaagtat tggagtcagc
     4201 tattgctaat gccgagcaca ataacggtgc ggacattgat gaactgaaag tggtaactat
     4261 ctttgttgac aaaggcccaa gcttgaaacg ttttcaagct cgcgccaaag gtcgcggtaa
     4321 ccgcatcgaa aaacaaactt gtcatatcaa tgtgacagtg ggtaactaag gaaaagctat
     4381 gggacaaaag attaacccta caggctttcg cctggcggta actaaagact gggcttcaaa
     4441 atggtttgct aaaagcaccg acttttctac tgttttgaag caggatatcg atgttcgcaa
     4501 ttatttgcgt caaaaattgg ccaatgcttc ggttggtcga gtggttattg aacgccctgc
     4561 aaaatctgca cgcattacca ttcactccgc tcgtccgggt gtggttatcg gtaaaaaagg
     4621 tgaggatatc gaggttttga aacgtgactt gcaagtcttg atgggtgtac ctgttcatgt
     4681 aaatattgaa gagatccgcc gtcctgagtt ggatgctcaa attattgctg acggtattgc
     4741 ccagcagttg gaaaagcgcg ttcaattccg tcgtgctatg aaacgagcaa tgcaaaatgc
     4801 aatgcgttct ggtgctaaag gcattaagat tatgacttca ggccgtctga atggtgcgga
     4861 tattgcccgt agcgaatggt atcgtgaagg tcgcgtgcca ttgcatactt tacgtgcaaa
     4921 tgtagattat gcaaccagcg aagcgcacac cacatatggt gtattgggtc tgaaagtttg
     4981 ggtttatacg gaaggcaata ttaaatcttc caaacctgaa catgagagta aacaaagaaa
     5041 ggcaggtaga cgtaatgctg cagccaacta gactgaaata ccgtaagcaa caaaagggtc
     5101 gcaatactgg catcgctact cgcggtaata aggtaagttt cggtgagttc ggcttgaaag
     5161 ccgtaggtcg tggtcgtttg actgcccgtc aaatcgaagc tgctcgtcgt gcaatgaccc
     5221 gtcatatcaa acgtggtggt cgtatttgga ttcgtgtatt ccctgataaa ccgattactg
     5281 aaaagcctat tcaagttcgt atgggtggcg gtaaaggtaa cgtggaatat tacattgccg
     5341 aaattaaacc aggtaaagtg ttgtatgaaa tggatggcgt tccagaggaa ctggctcgtg
     5401 aagcattcga gttggctgct gccaaattgc ctattcctac aacctttgta gtaagacagg
     5461 tgggtcaata atgaaagcaa atgaattgaa agacaaatcc gttgagcagt tgaatgcaga
     5521 tttgttggac ttgttgaaag ctcagtttgg cttacgtatg caaaacgcta ccggtcaatt
     5581 aggcaaacca agtgaattga aacgtgtacg tcgcgatatt gctcgtatta aaaccgtttt
     5641 aactgaaaaa ggtgctaagt aatgagcgaa actaaaaatg ttcgtacttt gcaaggcaaa
     5701 gtagtaagcg acaaaatgga taaaaccgta acagtattgg ttgagcgtaa agtaaaacat
     5761 ccgctgtatg gtaagattat tcggttatct actaaaatcc atgcccatga tgaaaataat
     5821 caatatggaa ttggtgatgt ggttgttata tcggaatccc gtccattgtc aaaaactaaa
     5881 tcttgggttg tcagtgagct ggttgagaaa gcacgttcta tttaagaatt aaagcaacgt
     5941 gcttggaatg ggaaacgaag tattgcagca aatttaattt gcgtgtaaac ttcgtttcct
     6001 gtctttcagt ttcttctgga agtttcttcc ctttcggggt ccaagactgg tttacttgaa
     6061 ccgcaaggtt tcatttaata agcagcggct ttgctgtaag ttatctgaaa gtggtaaatt
     6121 aagttggtta atttaaaggt aataacatga ttcaaatgca gaccatctta gatgtggctg
     6181 ataactctgg tgcgcgtcgc gtaatgtgta tcaaggtatt gggcggatct aagcgtcgct
     6241 acgcttctgt tggcgatatt attaaagtgg cagttaaaga tgcggctccg cgtggccgtg
     6301 tcaaaaaagg cgatgtatat aatgcggtag ttgttcgtac tgctaagggt gtacgtcgtc
     6361 ctgatggtgc gttaattaaa ttcgataaca atgccgccgt gttactgaat aataaacttg
     6421 aacctttggg tactcgtatc tttggtccgg taacccgtga attgcgtact gagcgattta
     6481 tgaaaatcgt ttcattggca cctgaagtat tataaggaat ggcacgatga ataaaatcat
     6541 taaaggcgat agggttgtag taattgctgg taaggataaa ggtaagcagg gtcaagtagt
     6601 tcgagtgttg ggtgataaag ttgttgttga gggcgttaat gttgtaaaac gccatcaaaa
     6661 acctaatcca atgcgtggca ttgagggcgg tattattact aaagaaatgt ctttggatat
     6721 ttctaatatc gcaatcctga atccggaaac taataaagcg gaccgtgttg gtattaagct
     6781 gattgaaaat gaaggcaaag ttaaacgcgt tcgtttcttc aaatcaaatg gctctatcat
     6841 tggggcataa ggagataaca tggctcggtt gagagagttt tataaagaga cagttgttcc
     6901 tgaattggtt aaacaatttg gttacaaatc agtaatggaa gtcccgcgta ttgaaaaaat
     6961 caccttgaat atgggtgtgg gtgaggctgt tgctgacaaa aaagttatgg aacatgctgt
     7021 ttccgattta gagaaaattg ccggtcaaaa accggttgtt actgttgccc gtaaatctat
     7081 cgcaggtttt aaaatccgtg ataactatcc ggttggttgc aaagtaacat tgcgtcgtga
     7141 tcaaatgttt gaattcttgg atcgtttgat tactattgca ttacctcgcg tacgtgactt
     7201 ccgtggtgtg agcggtaaat catttgatgg ccgtggcaat tacaatatgg gtgttcgtga
     7261 gcaaattatt tttccggaaa ttgaatacga taaaattgat gctttgcgtg gtttgaatat
     7321 tactattact actacagcaa aaaccgatga ggaagcgaaa gctttattgt cattgtttaa
     7381 atttccgttc aaaggataat catggctaag aaagcactta ttaatcgtga tctgaaacgt
     7441 caagctttgg ctaaaaaata tgcggctaaa cgcgcggcaa ttaaagcggt aatcaatgat
     7501 tcgaatgcaa ctgaggaaga gcgttttgag gctcgtttga ggtttcaatc cattcctcgt
     7561 aatgcggcac ctgtgcgtca acgtcgtcgt tgtgctttga caggtcgccc tcgtggtact
     7621 ttccgtaaat ttggtttggg tcgtattaaa atccgtgaaa tcgccatgcg tggcgaaatt
     7681 ccgggtgttg ttaaagccag ctggtaatag gagtaattaa gaatgagtat gcatgatcct
     7741 atttccgata tgttgactcg tatccgcaat gcgcaacgtg ctaataaagc agcagttgca
     7801 atgccttctt caaaattaaa gtgtgctatt gcaaaggtat tgaaagaaga aggatatatt
     7861 gaggacttcg cagtttcatc tgacgtaaag tctatattgg aaattcaatt aaaatactat
     7921 gcaggtcgtc ctgtaattga acaaatcaag cgtgtatctc gccccggttt gcgtatttat
     7981 aaagcgtcta gtgagattcc aagtgttatg aatggcttgg gtattgctat tgttagtact
     8041 tctaaaggtg taatgactga tcgtaaagca cgttctcaag gtgttggtgg tgagttgtta
     8101 tgcattgtag cctagtggag gaaaagaaat gtcacgtgtc gcaaaaaacc cagtgactgt
     8161 tcccgctggt gtagaagtaa aatttggaac agaggcatta gttattaagg gtaagaacgg
     8221 tgaattgtct tttcctttgc attctgatgt agccattgaa tttaatgatg gcaaattgac
     8281 ttttgttgcg aataacagca gtaaacaagc aaatgcaatg tctggtactg ctcgcgcatt
     8341 agtcagcaat atggttaaag gtgtttcaga aggttttgag aaaagattgc aattgatagg
     8401 tgtgggttat cgtgctcaag cacaaggtaa aatcttgaat ctgtctttgg gtttttctca
     8461 tccgatcgta tatgaaatgc ctgaaggtgt ctccgttcaa actcctagcc aaacagagat
     8521 tgttttaacc ggctcggata aacaagttgt tggtcaagtt gctgctgaga ttcgtgcgtt
     8581 ccgtgctcct gagccttata aaggtaaagg tgttcgctat gtaggagaag tagtggtaat
     8641 gaaagaagcc aagaaaaaat aattgaggtt cactaatgga taaacataca acccgactcc
     8701 gtcgtgcacg caaaacccgt gctcgtattg cggacttgaa aatggtaaga ttatgtgtgt
     8761 tccgaagcaa taatcatatt tatgctcaag taattagtgc tgaaggtgat aaagtattgg
     8821 ctcaagcctc tacattggaa gctgaggtgc gcggtagtct gaaatttgga agcaatgttg
     8881 aagcagctgc aatagttggt aaacgtatcg ctgagaaggc taaagcagca ggtgtagaaa
     8941 aggttgcttt tgatcgttca ggtttccaat atcacggtcg tgtgaaggct ttggctgaag
     9001 ctgctcgtga aaatggttta agcttctaaa tatttggaga ctttcagatg gcaaaacatg
     9061 aaattgaaga acgcggtgac ggtctgattg aaaagatggt cgcagttaac cgtgtaacca
     9121 aagtagttaa aggtggtcgc attatggctt tctctgcgct aactgttgtt ggtgatggag
     9181 atggtcgtat tggtatgggt aaaggtaagt caaaagaagt gcctgttgca gtccaaaagg
     9241 cgatggatca agcacgacgc tctatgatta aggtaccatt aaaaaatggt acgattcatc
     9301 atgaggttat tggtcgacat ggtgctacta aagtatttat gcagcctgct aaagagggta
     9361 gtggcgtaaa agccggtgga cctatgcgtt tggtttttga tgctatgggc attcataata
     9421 tctccgccaa agtgcacgga tctactaacc catataatat cgtacgtgca acattagatg
     9481 gtttgtctaa gttgcatact cctgctgata tcgcagccaa acgtggcttg acagtggaag
     9541 acattttggg agttaaccat ggctgaacaa aaaaagatta gggttacatt ggttaaaagc
     9601 ctgattggta caattgaatc tcatcgtgca tgtgcacgcg gtttaggttt gcgtcgtcgc
     9661 gagcatacgg tagaggtttt agatacccct gaaaaccgtg gtatgattaa taaaatcagc
     9721 tacttgttga aagtggagtc ttgatatgtt tttgaataca attcaacctg ctgttggtgc
     9781 tacgcatgct ggtcgtcgtg ttggacgcgg tattggtagt ggtcttggca aaacgggtgg
     9841 tcgtggtcat aaaggtcaaa agagccggtc tggtgggttt cataaggtgg gtttcgaggg
     9901 tggtcaaatg cccttgcaac gacgcctccc taaaagaggt tttaaatctt taacagcatc
     9961 agctaatgca cagcttcgtt taagtgaact ggaatcaatt gctgttaatg agattgatat
    10021 tttggtctta aagcaagcgg gtctgattgc atctacagtc tctaatgtta aagttattgc
    10081 ttctggtgaa atttctaagg cagttgcttt gaagggtatt aaagttacca aaggtgcgag
    10141 agctgctatc gaggctgttg gtggtaagat tgaaatgtaa ggtttaatat tgtggctaat
    10201 caacaaacgt catcaggttc atccaaattt ggagatctta agaaacgtct tttgtttcta
    10261 tttggagcat tgattgtttt tcgaattggt gcccatatac ccgtacctgg agttgatgct
    10321 gttgctttag ctaaattata cgaaagcgct ggaaacggca tcctgggaat attgaatatg
    10381 ttttccggtg ggtcgttaga gcgctttagt atatttgcaa taggaattat gccatatatt
    10441 tcagcttcta ttattgtaca gctcgcttct gaaattttgc catcattgaa ggctttaaaa
    10501 aaagaagggg aggctggtag aaaggtaatt acgaaatata ctaggtatgg tactgttttg
    10561 ttagcaattc ttcaaagtct aggtgttgca tctttcgtat ttcagcaagg aattgttgta
    10621 acaagttcat ttgagtttca tgtttccacg gtagtttctt tggtaacggg aaccatgttt
    10681 cttatgtggc ttggggagca aattactgaa aggggtatcg ggaacggtat ttctttaatc
    10741 attacggcag gtattgcttc aggtattcct tcgggtattg caaagctggt tacactgacg
    10801 aaccaaggtt ctatgagcat gcttacggcg ttgtttattg tatttggtgc cttattatta
    10861 atttatttgg ttgtatactt tgaaagtgca cagcggaaga ttcctattca ttatgcaaaa
    10921 cgccagttta atggtagggc gggtagtcaa aatacgcata tgcctttcaa gttgaatatg
    10981 gctggtgtta ttcccccaat ttttgcttcc agtattattc tatttccatc tactctttta
    11041 ggttggtttg gttcggctga tacaaatagt gttttgcaca aaatagctgg attgttacaa
    11101 cacggtcaat tgctgtatat ggctttattt gcagcgacag ttattttctt ttgttatttt
    11161 tatacggctt tggtttttag ccctaaagaa atggcagaga atttaaaaaa gagtggtgct
    11221 tttgttcctg ggattagacc tggtgagcag acctctaggt atttagaaaa agttgtatta
    11281 cgtttgacat tgtttggagc tctttatatt acaactattt gtttaattcc agagttctta
    11341 actacggttt taaatgtacc tttttatttg ggtggcacgt ctttgttgat tctagttgtt
    11401 gtaacgatgg attttagtac acaaataaat tcgtataggc ttactcaaca gtatgataag
    11461 ttaatgactc gttcagaaat gaaatcattt tctcggaaat agaattatgg cgaaagaaga
    11521 tactatccaa atgcaaggtg aaattcttga aactttacct aatgcaacat ttaaagtaaa
    11581 acttgagaat gaccatattg tattgggtca tatttctggg aagatgcgga tgcattacat
    11641 tcgtatttct ccgggagata aggtcacagt agagctgaca ccttatgatc taactagggc
    11701 tcgaatcgtt ttcagagcaa gataaaccaa taaaaggaaa ataaaatgcg tgtacaacca
    11761 tctgttaaga aaatttgccg aaattgcaag attattcgtc gaaatcgtgt agttcgtgta
    11821 atttgtactg atctccgtca caaacagcgt caaggttaat ggaatatttc ttttaatgtg
    11881 attctgtgat atagtgacac actttgccct aaaaaggaaa aaatatggct cgtattgcag
    11941 gggtaaatat ccctaataac gcacacatcg taattggtct tcaggctatt tacggtattg
    12001 gtgctactcg tgctaaattg atttgtgagg ctgcaaatat tgcgcctgat actaaagcaa
    12061 aagatttgga cgagactcaa ttagatgctt tgcgtgacca agttgccaag tatgaagtag
    12121 aaggtgattt gcgtcgtgag gtaactatga gtatcaagcg attgatggac atgggctgct
    12181 atcgtggctt ccgtcatcgt cgcggcttac catgccgcgg tcaacgcact cgtacaaatg
    12241 cgcgtacccg caaaggtccg cgtaaagcga ttgctggtaa gaaataaatt ttaaggaatt
    12301 ttattaatgg ctaaagcaaa cacagcttca cgtgtacgta aaaaagtacg taaaaccgtg
    12361 agtgagggta ttgtgcacgt tcatgcatct ttcaacaata ccatcattac aatcactgac
    12421 cgtcaaggca atgcgttgtc ttgggctacc tctggcggcg ctggttttaa aggttctcgt
    12481 aaaagtacac catttgcagc acaagttgca gcagaagcag ctggtaaagt tgcccaagag
    12541 tatggcgtta aaaatttaga ggttcgtatt aaaggtccag gtccaggtcg cgaatcctct
    12601 gtacgtgctt tgaatgctct tggtttcaag attaccagca ttactgacgt taccccgttg
    12661 cctcataacg gttgccgtcc gcctaaaaaa cgtcgtattt aatattggag tgatttgaaa
    12721 catggcacgt tatattggcc ctaaatgtaa gttggcacgt cgcgaaggta cggatttgtt
    12781 tttgaagagt gcgcgccgct ctttggattc taaatgtaaa attgattccg ctcctggtca
    12841 gcatggtgca aaaaaaccgc gtttgtcaga ctatggtttg cagttgcgtg aaaaacaaaa
    12901 aatccgccgt atttatggcg tattagaacg tcagttccgt cgttatttcg cagaagctga
    12961 tcgtcgtaaa ggttctaccg gcgagttgct gttgcagttg ctggaatctc gtttggataa
    13021 tgtcgtttat cgtatgggtt tcggttctac ccgagctgaa gcaagacagc ttgtttctca
    13081 taaggcgata gttgtgaatg gacaagttgt caatattcct tctttccaag tgaaagctgg
    13141 tgatgttgtc tcagttcgtg aaaaagccaa aaaacaggta cgtattcaag aagcattggg
    13201 tttggcaact caaatcggct tgccgggttg ggtttctgta gatgcggata aacttgaggg
    13261 tgtgttcaaa aacatgccgg atcgctcgga attgaccggt gatattaatg aacagctggt
    13321 ggtagagttc tactctaaat aatgctagct cagtgaggga cagttaaatg cagaatagca
    13381 caaccgaatt tttgaaacct cgtcaaattg atgtaaatac tttttctgca actcgtgcaa
    13441 aagtatctat gcagccattt gaacgtggtt tcggtcatac cttaggtaat gctttgcgcc
    13501 gtatcttact gtcatccatg aatggttttg ctcctactga agtagctatt gccggtgtat
    13561 tacacgaata ttctactgtt gatggtattc aggaagatgt tgttgacatt ttgctgaata
    13621 ttaaaggtat tgtgtttaaa ctccatggtc gtagccaagt tcaacttgtg ttgaagaaat
    13681 caggttcagg tgtcgtatct gccggtgata ttgagttgcc gcatgatgta gaaattctga
    13741 atcctggtca tgtcatttgt catttggctg ataacggtca aattgagatg gaaattaaag
    13801 tagagcaagg tcgtggttat caatctgttt caggtcgtca ggtagttcgt gatgagaacc
    13861 gtcagattgg tgcaatccag ttggatgcga gcttttcgcc catcagccgt gttagctttg
    13921 aggttgaacc tgcacgtgta gagcagcgga cggatcttga taagttggtt ttggatatcg
    13981 aaaccgacgg ttctattgat cctgaggaag ctgtacgcag tgcggcacgt attttgattg
    14041 atcagatgtc tatttttgct gatttgcagg gtacgcctgt ggaggaggtt gaagaaaaag
    14101 cacctcctat cgaccctgtt cttttgcgtc cggtggatga tctggaattg acagtacgtt
    14161 cagctaattg tttgaaagct gaggatattt attatattgg cgatttgatt caacgcactg
    14221 aaaccgagct tcttaaaacg ccgaatttgg gacgtaaatc tttgaatgag attaaggaag
    14281 tattggcatc taaaggtttg acactgggtt ctaagttgga agcatggcca cctgtaggct
    14341 tggaaaagcc ttaatgaaga attaaaggat aattgatatg cgtcatcgta atggcaatcg
    14401 caaattaaac cgtaccagca gtcatcgtgc tgcaatgctg cgtaatatgg cgaattcatt
    14461 attgactcac gaaactattg taacaactct gcctaaggcc aaggaattgc gccgtgtagt
    14521 agagccgttg attacattgg gtaaaaagcc gtcattggca aaccgccgtt tggcatttga
    14581 ccgtactcgc gaccgtgatg ttgtagtaaa actgtttggc gatttgggtc ctcgttttac
    14641 tgctcgtaac ggtggttatg ttcgggtgtt gaaatacgga ttccgtaaag gtgataatgc
    14701 acctctggca ctggttgaat tggttgacaa accggctgct gagtaatttt agtcatataa
    14761 cgccatctgc cgaaaagcag gtggcgttat ttttgcaata tctgataggt aatagggtat
    14821 tggctatcat gtttaaaata ttaattgaat agctaaggtt tgcgcggtaa acttacatca
    14881 ttaaaaaatt ctatgatggt ttatataatg aatgctttcg atataaagtc gacaaagatg
    14941 gacgtattgt ctatatcttt gcatacgtca gacttgtttg atttggaaga tgtgctggtc
    15001 aaattgggca agaagtttca agagtctggt gttgttccat ttgtgctgga tgttcaagag
    15061 tttgattatc ccgagtcttt ggatcttgct gcattggttt cgttgttttc aaggcatggt
    15121 atgcaaattt tgggtctgaa gcattctaat gaacgttggg ctgctgcggc tatgaagtat
    15181 catttgctgt tttgtctgtc tcattcggaa aatgttaaag aactgggtca ggttgaggtg
    15241 cagaacacgg aggatggtca gaaagcaagg aaaacagtat tgattacatc ccctgtccgt
    15301 accggtcagc aggtttatgc cgaagatggc gatttgattg ttacgggggc ggtcagccag
    15361 ggggcggaat tgattgcgga tggcaatata catatttatg cgccgatgag ggggcgtgct
    15421 ttggccggtg ccaagggtga tacttctgcc cgcatattta tccactccat gcaggcagaa
    15481 ctggtttctg tggcgggtat ttaccgtaat tttgaacagg atttgccgaa ccatctgcac
    15541 aagcagccgg tacagatatt gttgcaggat aaccgattgg ttatcagtgc aattggctca
    15601 gagtaattgt ttgatattta aaaaggaaat attgtggcaa aaattattgt agtaacttca
    15661 ggtaagggcg gtgtcggtaa aacgactacc agtgccagta ttgcgacagg tttggcatta
    15721 cgcggatata aaactgcggt aattgatttt gatgtgggtt tgcgtaacct cgacctcatt
    15781 atgggttgcg agcgtcgtgt cgtttatgac ctgatcaatg tcattcaggg ggaggcgacg
    15841 ctcaaccaag ctttgattaa agataaaaat tgtgaaaacc tgtttatttt gccggcttcc
    15901 cagactcggg ataaagacgc tttgacacgc gagggcgtag aaaaagtgat gcaggagctg
    15961 tccggcaaga aaatgggctt tgagtatatt atttgcgact ctcctgccgg tattgagcag
    16021 ggtgcattga tggcgttgta ttttgctgat gaagccattg taacgaccaa tcctgaggtt
    16081 tccagtgtgc gtgactccga caggattttg ggaattttgc aaagcaaatc ccataaggca
    16141 gagcaaggcg gttcggttaa agaacatctg ttgattacgc gttattctcc cgaacgtgtg
    16201 gcaaaaggcg aaatgctgtc tgtacaggat atttgcgata ttctgcatat tcctttgctg
    16261 ggtgtgattc ctgaatccca aaacgtcttg caggcatcca attccggaga accggtcatc
    16321 catcaggaca gcgtggcggc ttccgaggca tataaggacg ttattgcccg tcttttgggc
    16381 gagaaccgtg aaatgcgttt cttggaagct gagaaaaaaa gcttcttcaa acgtctgttt
    16441 ggaggataag gtatgtcatt aatcgaattt ttattcggca gaaagcagaa aacggcaacc
    16501 gttgcccgcg accgccttca aatcatcatt gcccaagagc gcgcccaaga aggtcaggct
    16561 ccggattacc tgccgacttt acgtaaagag ttgatggaag tcctgtccaa atatgtgaat
    16621 gtttcattag acaatatccg tatttcccag gaaaaacagg atggtatgga tgtgcttgag
    16681 ttgaacatta ctttgccgga acagaaaaag gtataggaca tgaccttaac cgaattgcgg
    16741 tacatcgtcg cagtcgccca agaacgtcat ttcggcaggg cggcgcggcg ttgttttgtc
    16801 agccagccca ctttgtctat tgccattaag aaattggaag aagagcttgc cgtctctttg
    16861 tttgaccgga gcagcaacga tattattacg accgaggcgg gggaacgtat cgttgcacag
    16921 gcgcgtaagg tgttggaaga ggcagagctt atcaggcatt tggcaaatga agaacaaaac
    16981 gagctggagg gtgcgttcaa actcgggctg atttttacgg ttgcgccgta cctgctgccg
    17041 aaactgattg tctcgttgcg ccgtactgca ccgaaaatgc ctttgatgtt ggaagagaat
    17101 tacacgcata ctttgaccga gtcgctcaaa cgcggggacg ttgatgcgat tatcgttgcc
    17161 gaaccgtttc aagagccggg cattgttacc gaacccttgt atgacgaacc gtttttcgtg
    17221 attgtcccga aagggcattc atttgaggaa ctggatgccg tttcgccccg gatgctgggt
    17281 gaggagcagg ttttgctgct gacggaaggc aactgtatgc gggatcaggt actctcaagc
    17341 tgttccgaat tggcggcgaa acaacgtata caggggttga ccaatacatt gcagggcagc
    17401 tcgattaata caatccgcca tatggttgcc agcggtttgg caatcagcgt gttgccggca
    17461 accgcactga ccgaaaacga tcatatgctg ttcagcatta ttccgtttga gggtacgccg
    17521 ccaagccggc gggtcgtatt ggcgtaccgc cgcaattttg tccgtccgaa ggcgttgtcg
    17581 gcgatgaagg cggcgattat gcagtcgcag cttcacgggg taagttttat ccgcgactag
    17641 gcgcaggcat tgttttcaaa acgccatttc cctgagccga caacacggta tgccaagata
    17701 ttgccgtcat catcgatttt gagtatagca tcgccacgga aactgccgtc ctgaagatat
    17761 tcgacttttg catcactgtg aatgttttca tcagtgccga tgcaatgcca tgtatagtgg
    17821 attaacaaaa accagtacgg cgttgcctca ccttgccgta ctatttgtac tgtctgcggc
    17881 ttcgtcgcct tgtcctgatt tttgttaatc cactataaaa gaggccgaga cctttgcaaa
    17941 actccccaaa atcccctaaa ttcccaccaa gacatttagg ggatgttcca tgagcacctt
    18001 cttcc
//
LOCUS       DAWKST010000024        17783 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.024, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000024 DAWKST010000000
VERSION     DAWKST010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17783
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.024"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..551)
                     /locus_tag="WJL87_000581"
     CDS             complement(<1..551)
                     /locus_tag="WJL87_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEZ7799050.1"
                     /translation="MQTANKKTILVTGGAGFIGSAVVRHIIRNTQDSVVNLDKLTYAG
                     NLESLTDIADNPRYAFEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIGSAGEF
                     IQTNIVGTFNLLEAARAYWQQMPSEQHEAFRFHHISTDEVYGDLGGTDDLFTETAPYA
                     PSSPYSASKVSSDHLVRAWLRTYG"
     gene            complement(552..1595)
                     /gene="galE"
                     /locus_tag="WJL87_000582"
                     /pseudo
     CDS             complement(552..1595)
                     /gene="galE"
                     /locus_tag="WJL87_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223275.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
     gene            complement(1588..2590)
                     /gene="dcm"
                     /locus_tag="WJL87_000583"
                     /pseudo
     CDS             complement(1588..2590)
                     /gene="dcm"
                     /locus_tag="WJL87_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(2621..4894)
                     /locus_tag="WJL87_000584"
     CDS             complement(2621..4894)
                     /locus_tag="WJL87_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ7799051.1"
                     /translation="MNITQILSQELSATTAQINAAIELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPVRTMPGHRA
                     LAVLRGRNEGVLNIALKYRPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYKNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(4959..5609)
                     /locus_tag="WJL87_000585"
     CDS             complement(4959..5609)
                     /locus_tag="WJL87_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7799052.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            complement(5606..6403)
                     /locus_tag="WJL87_000586"
     CDS             complement(5606..6403)
                     /locus_tag="WJL87_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ7799053.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLKADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGDSVTTYENPWYILLCNLVLLLLGLAVVARFSKGVEPQ"
     gene            complement(6403..7566)
                     /gene="ctrB"
                     /locus_tag="WJL87_000587"
     CDS             complement(6403..7566)
                     /gene="ctrB"
                     /locus_tag="WJL87_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HEZ7799054.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(7581..8756)
                     /gene="ctrA"
                     /locus_tag="WJL87_000588"
     CDS             complement(7581..8756)
                     /gene="ctrA"
                     /locus_tag="WJL87_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ7799055.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            8883..10016
                     /gene="neuC"
                     /locus_tag="WJL87_000589"
     CDS             8883..10016
                     /gene="neuC"
                     /locus_tag="WJL87_000589"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEZ7799056.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHPDLELHLIVTGMHMMKT
                     YGRTYKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITFISRLSDEIEPDMVMIHGDRL
                     EALAGATIGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALELSGQNIISIYPNNDTGTESILQELLKYQSDKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            10020..10706
                     /gene="neuA"
                     /locus_tag="WJL87_000590"
     CDS             10020..10706
                     /gene="neuA"
                     /locus_tag="WJL87_000590"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEZ7799057.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            10707..11756
                     /gene="siaC"
                     /locus_tag="WJL87_000591"
     CDS             10707..11756
                     /gene="siaC"
                     /locus_tag="WJL87_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HEZ7799058.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIE"
     gene            11836..13314
                     /locus_tag="WJL87_000592"
     CDS             11836..13314
                     /locus_tag="WJL87_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2,8-polysialyltransferase family protein"
                     /protein_id="HEZ7799059.1"
                     /translation="MLQKIRKALFHPKKFFQDSQWFATPLFSSFAPKSNLFIISTFAQ
                     LNQAHSLTKMQKLKNNLLVILYTTQNMKMPKLIQKSVDKELFSVTYMFELPRKPGIVS
                     PKKFLYIQRGYKKLLKTIQPAHLYVMSFAGHYSSLLSLAKKMNITTHLVEEGTATYAP
                     LLESFTYKPTKFEQRFVGNNLHQKGYFDKFDILHVAFPEYAKKNFNANEYHRFFAHSG
                     GISTSQSIAKIQDKYRISQNDYIFVSQRYPVSDEVYYKTIVETLNQMSLRIEGKIFIK
                     LHPKEMENKNIMSLFLNMVTINPRLVVINEPPFLIDPLIYLTTPKGIIGLTSTSIVYT
                     PLLSPTTQCLSIGQIVIDSIHHTAQQENTALIEEHLEIVKQFDFIKILSSIEDGIDTN
                     SFKTEETLEMLLKSAEYAYKNKNFYQAIFYWQLASNNDLSVLGYKSLWYYNALNKVKQ
                     NYKMKYLEINYIERISLYFNDKDKMIWQNIKNDFFKYSLCNQ"
     gene            13343..14725
                     /locus_tag="WJL87_000593"
     CDS             13343..14725
                     /locus_tag="WJL87_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799060.1"
                     /translation="MSINTFETFIYELYQSTNFAEIARITGFSNHKALNLETIRMMAI
                     SCFKTKNTTKCIELSEYFNEKSDIKDFFIFEILAECYFLQNDLVKSLENYEKALLLNP
                     KLISARFKHMCLKYRLFNELDSTTFSKYEIESQQKKKISNLRIIAYIQLKEKKYFKAY
                     NNLKLLIELNKSPFYPDYLSIIHAIENLEYSKQSQNTKKELTEYIKISKAIALKSEFV
                     CNGSNNLFVTLSPATGFVLKKYNYPADKLCFIDNTNTYYTFAYELIAEHIISLVKKYK
                     YENISIIGSSKGGTATIILLNLLQTALPNTTICAVSCSPQIQIFPFNKNLTIPSYQKF
                     AEYFSYNSILESKCAQAQKLINFDILYRNKLTIFYGDKFKMDAQEVSTIRPINNTTII
                     PLNYSGHGSLIPLTIPENKSFDELKQKYSKLEIDTDFQALGGNNLSSIVDEIFEIYSN
                     PDMRLHKFLC"
     gene            complement(14776..15732)
                     /locus_tag="WJL87_000594"
     CDS             complement(14776..15732)
                     /locus_tag="WJL87_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799061.1"
                     /translation="MHQYYDAVILSVGNGLLKRFFKQNAQLNIASRPLIITLFPGVVF
                     GDQASILSRMGADIVLYNNKHDFRIAETYKKQYKLSCQNILYGYPNFRHASKGCHGER
                     IYFIDQVKIPFKKEERIYTLKKLIALAEKYPEKEFTILLRVADKDITVHQDKHSYIEL
                     AKQFQLPSNLTIERKSTAQAFQEMGYCLSYSSTMLFEAECKGIPVGVVADLGFSKSYA
                     NQHFLGSGVLIYFDQIDFTSPKIANPGWLDCYATKKVITTDEFNKLLKQVVPLQHDYQ
                     EYLFAVNSIESTQTIFLRKFKKLIRDPKQFFYDSKWFRRRNS"
     gene            16113..17132
                     /gene="galE"
                     /locus_tag="WJL87_000595"
     CDS             16113..17132
                     /gene="galE"
                     /locus_tag="WJL87_000595"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ7799062.1"
                     /translation="MKKILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPR
                     LKTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPMKYYDNNV
                     SGSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPLGDTTSPYGTSKSMVERI
                     LTDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVASGRLPQLSV
                     FGGDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEIIRA
                     FEAASGLTIPYEVKPRRAGDLACFYADPSYAKAQIGWQTQRDLTQMMEDSWRWVSNNP
                     NGYDD"
     gene            17248..>17783
                     /locus_tag="WJL87_000596"
     CDS             17248..>17783
                     /locus_tag="WJL87_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEZ7799063.1"
                     /translation="MRKILVTGGAGFIGSAVVRHIIRNTQDSVVNLDKLTYAGNLESL
                     EPVSHHPRYAFEQVDICDRAALDRVFAQHQPDAVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWHDMPSEKKAAFRFHHISTDEVYGDLSGTDDLFTETTPYAPSSPY
                     SASKASSDHLVRAWQRTYG"
BASE COUNT         5177 a         4088 c         3572 g         4946 t
ORIGIN      
        1 ccgtaagtac gcaaccacgc gcggacgagg tggtcgctgg acactttaga ggcagagtag
       61 gggctggacg gcgcgtaggg cgcggtttcg gtaaacaaat cgtccgtgcc gcctaaatcg
      121 ccatagactt catcggtgga aatatggtgg aaacggaagg cttcgtgctg ttcagacggc
      181 atttgttgcc agtaggcgcg ggctgcttca agcagattga atgtgccgac gatattggtt
      241 tggataaact cgcctgccga accgatagag cggtcgacat ggctttccgc cgccaagtgc
      301 atcacggcat cgggccggtg ttgtgcgaat acgcggtcga gttcggcgcg gtcgcaaatg
      361 tccacttgct cgaaagcata gcggggatta tcggcaatgt cggtcagcga ttcgagattg
      421 cccgcgtagg tcagtttgtc gaggttgacg acggaatctt gggtgtttcg gataatatga
      481 cggacaacgg cagaaccgat aaagcccgca ccgccggtaa cgaggatggt ttttttgttt
      541 gcggtttgca tttaatcccc atatccgttg gggttgcggc tgacccaacg ccacgaatct
      601 tccatcattt gctgcaagcc gcgtttggtt tcccagccgg tttgttgttt ggtatgggac
      661 gggtcggcaa aggaacacgc caaatcaccg gcgcggcgcg gtttgatttc aaatggaatg
      721 gttaaacccg aagccgcttc aaatgcgcgg atgatttcca ataccgaaga agcgcggccg
      781 gagcctaagt tcagcaaatg cgtgcctgct acattacttt ttgcctgcat agccgcgaca
      841 tggccttctg ccaaatccat cacatgaata tagtcacgca tccccgtgcc gtcgggggta
      901 gggtagtcat cgccaaatac cgccaattgc ggcagtttgc ctgccgccac ttggcagata
      961 taaggcaaca aattattcgg gatgccgttt ggctgctcgc caatcaaacc actttcgtgc
     1021 gccccaatcg ggttgaaata gcgcaacaaa atcacgctcc aacgcggatc ggccttttgg
     1081 atgtcggtta acatccgttc caccatcgct ttggatgcgc cataaggatt agcggtatcg
     1141 cccgggcgca tatcttccgt atagggcatt ttacgtatca tggcgcgacg cattaaagta
     1201 aacgccaaag cgcgtttata ctggttatcc agtttcccta tatttttacg ttacaaatca
     1261 agttgttggt attcatcgtg aaaatagaat ttttcggcat aacgctgact cataaaaccg
     1321 gcatgcgggc tacctgaaaa aatcattgcg ccatcatcgt catttaatgt ttcatggttg
     1381 ttttctatta atgctaaagc aatttggtaa ttaattgcca aaatatcgtt agtaatcaca
     1441 cgatagccgc gttttttggc ttcgtaggca aaggaacagc ctccactaaa tacatctgca
     1501 actgtatcta catcagacgg aagctggtca caaatccagg aagctatttt ctctttatta
     1561 ccgatatagt taattttcgg atattgctta ttcattttca ctcattttta aaatactttc
     1621 agcaatagct tttgccaata aaggcggtac ggcgttacca acctgctgct gctgggcaat
     1681 tttgctgccg caaaaaataa aattatcagg gaaagattgt aaggcagcta attcacgaac
     1741 ggttaacgcc cgattctgtt catagtgaaa aactttgcgc atatctcctg taatacaaac
     1801 ggctggtttt gttgctgttg taacggatgt atttacggat atcacctgtt ttcggacgta
     1861 atggttcagg aatatcgtta cggttacctc catttttaac aaatgccatt ttttctaaca
     1921 tttgtgccga atgattcata gcttcatgat ttgcaacgtg tggattgctt tcgccagcag
     1981 ccagttttgg aaaatgtcct attgctgatc caacagtctg atgggaaatc tgcaaaggtt
     2041 cgggaaagga aattttgcct ttatccctcc tcccgataaa tatcactcgg ctacgtatct
     2101 gaggaacacc gaaatcggct gcactcagta tcttacattc caccgaataa ccgatattct
     2161 gaaatgcttg aataatctca atacgtgttt tacctgaatt gtgtgtatag agtcgcgcta
     2221 cattttccat aacaaaaaaa tatggttgga caattttaac tattcggaca aactctttaa
     2281 ataaatggtt gcgtgggtca tctgtaaatg tccgtccaat ctttcctgcc atactaaaac
     2341 cttgacaagg tggtcctcca ataatcaaat caactgcttg tccgttaaga caattgatta
     2401 aatcttgttc ggttagtgtg gttaaatctt tttgcagtaa ttgatgatgg gggaagttgg
     2461 tacggtaagt ctgacaataa tcagactcca tttcaacaga aagcaattgt tggaatccgg
     2521 cttgttcaaa acccaaggat aggcctcctg ctcctgagaa aaggtcaata taggtaagtg
     2581 gtgtgtgcat aagtcaaaat ccataaactc ttcgattatt ttaccgcttc agcttcgcaa
     2641 acgcatccgc catcgccgaa ttggctgggg cgcggtcgtt gcgttggggt ttgcggccgg
     2701 ctgtccgttc gcggctgcgg ttttcagacg gcattttatg ttttgcgccg cccggttcgt
     2761 catccaagcg catggtcagc gcgatgcgtt tgcgtgcagc atcgacttcc agcactttca
     2821 ctttcaccac gtcgccagct ttcaccactt cgcgcgggtc ttggacgaac ttgttggaca
     2881 gggcggagat gtgcaccaag ccgtcctgat ggacgccgat gtccacgaac gcgccgaagt
     2941 tggcgacgtt ggaaaccacg ccttcgagta tcataccgac ttgcaagtcg ctgatttcgt
     3001 ggataccttc ggcaaacgat gccgtctgaa actcgccgcg cggatcacgg ccgggttttt
     3061 ccagttcgga caggatgtcc aaaatggtcg gcaggccgaa gcgttcgtcg gtgaagtcgg
     3121 acgctttgat ttgcttcacg cgctcgcggt tgccgatgag ttcggcggcg ctaatgcctt
     3181 gttgcgccag cattttggcg acgacgggat aggcttcggg gtggacggcg ctcgcgtcca
     3241 acggctcttt gccgccgtta atccgcaaaa agcccgccgc ctgctcgaag gttttttcgc
     3301 ccaaacgcgg tactttcagc aattttttgc ggctgtcgaa cgcgccgttt tcatcgcggt
     3361 aggcgacaat gttttgggca agggtttgat tcaaaccgga aattcgcgcc aagagcgggg
     3421 cggaggcggt gttcacgtcc acgccgacgg cgttcacgca gtcttcgacc accgcgtcca
     3481 gcgatttggc gagctggctt tggttcacgt cgtgctgata ctgccccacg ccgatggatt
     3541 tggggtcgat tttgaccaac tcggcaagcg ggtcttgtag cctgcgggcg atggacaccg
     3601 cgccgcgcag ggaaacgtcc aagtcgggga actcgcgcgc cgccagttcg gacgcggaat
     3661 aaatcgacgc gccggcttcg gacacgacga ttttatgcag ccccattttc gacatttcac
     3721 gcaccagttc gcccgcgatt ttgtcggttt cacggctggc ggtgccgtta ccgatggcga
     3781 tgagcttcac gccgtgctgt ttaatcaggc gcgacagcgt tgccaacata ttgttttctt
     3841 gatgcaaata gacgatgacg gtatccagca gcttgcccgt gtcgtccacc acggcacatt
     3901 tcacgccgtt cttgtatcca gggtcgagac ccagcgtggt cagccgtccg gcgggtgcgg
     3961 cgagcagcaa gtctttgaga tttcgggcaa acacggtaat cgcgtcggtg tcggcggctt
     4021 ctttcaaacg attgagagct tcgagttcca acgacaaaaa gatttttgcg cgccaagtca
     4081 gacgcacggt gtcgcgcagc catttgtggc cgtctgaaac cttgaaacgg cgggcgatga
     4141 tttgctcgta ttcgctttgc tgcgtaatcg gcgtgtcgtc gggccggtat ttgagcgcga
     4201 tgttcaacac gccttcgttg cggccgcgca aaaccgccaa cgcgcggtgg ccgggcatag
     4261 tgcggacggg ttcgcggtgg tcgaaataat cgctgaattt ttcgccttcg gtttctttgc
     4321 ctccaacaac ttgcgcgtgg atttcggctt cgttccacag cttgtcgcgc agcgtgccga
     4381 tgagttccgc gtcttcggca aactgctcca tcagaatcgc acgcgcgccg tccaacgcgg
     4441 ctttggcatc ggggacgttt tcgttcaggt agccttgtgc ggcggcttcc acgtcctgcg
     4501 actgctcggc aagcaacacg tccgccagcg gctgcaaacc gtgttcgcgc gcgatttgcg
     4561 ctttggtgcg gcgtttgggt ttgtagggca gatacaggtc ttccagcgcg gttttgttat
     4621 cggcggcttc gatttgcgcc ctgaggtcgt ctgaaagctt gccttgctct tcaatgcttt
     4681 ttaaaacaac ggctttgcgc tcttccaact cgcgcaggta ttgcagccgc tcggcaagcc
     4741 ggcgcagctg cgtatcgtcc aacccgcccg tggcttcctt gcggtagcgg gcgataaagg
     4801 gcacggttgc gccgtcgtcc aaaagctcga tagcggcatt gatttgcgcg gtagtcgcgg
     4861 aaagttcttg ggaaagaatt tgagtaatgt tcatcaataa aattccaacg gacaggccgt
     4921 ctgaaatttc agacggcctg atttaaaaaa caatcgcttt aaggtagcga attataatat
     4981 tcgtaggctt tgtccatatc ttcaaactgg tacatatgcc ctttttccag caccatcgca
     5041 ttatcgcaat attgcttcat ggcgctgtgg ctgtgcgaca ccaagatgat ggaacggtct
     5101 ttgcgctttt caaacaactc gtacttacat ttatcggcaa aacgcgagtc accaactgca
     5161 atcacttcgt caatcaaata gcaatcaaac tctaccgcca acgacaaagc aaatgccaaa
     5221 cgcgccttca tacctgaaga ataacgcttg accggctcgt aaagatattg ccccaactcg
     5281 gaaaactctt cggtaaagtt cttcacataa tcgatatcga cattgtaaat ccggcagatg
     5341 aaacgcaaat tgtccatacc ggtcagactg ccttgaaacg caccggagaa tgccaaaggc
     5401 caagaaatac tcattgtccg cttgatttca cccgtggtcg gcggctcaac gccactgatc
     5461 aaacggatga gcgtcgattt acctgcaccg ttgcggccga gaataccgat tttctcgccc
     5521 ttctccattt tgaagctaat atcgtgcaag actgtccgcc aaccttggcg ggtcagatag
     5581 cgtttggaaa cgtgttcaac tgaaatcatt gcggctcaac ccctttgctg aatctcgcca
     5641 ccaccgccaa accaagcagc agcaaaacca agttacacaa caaaatatac caaggatttt
     5701 cgtaagtcgt gacgctgtcg ccaaaatatc cggcacggaa catttctgtg ccatgcacca
     5761 tcgggatcat caatgcatac tcttgcactt tcggaggcaa attatgtaca aaaaagaatg
     5821 cacccgataa cggcatcatc acaaaagtca atgtgcccca aatcttgcca aacggctcga
     5881 aattaaaggc aatcgaacaa atcaccaaac ccaaaccaat cgcaaaaaaa gccatcaaaa
     5941 gccaagccat cagcatataa aacatatctg ccggcatttc aatccagcca atcgcaatca
     6001 ataccgccat aatcacaatc tgcgcaatgg ttgcaccagc aatttccaaa atcatgcgcg
     6061 ccaagatggt atccaaaact cttacattgc ggtgataaag caagctggca tttgaagaaa
     6121 tcgacccaac tgcccgtttt gaggcattac gccacatcat caacatcgga tagccagtaa
     6181 tcgcaaatgc gacaatattc aaagttgaat atcggtctgc ctttaaaaat ttccacatca
     6241 agacgataac gaatgtcatc agcaacggct caacaaacag ccataaaaag ccaatattat
     6301 tgcgaccgta acgggtgata atttcccgca tcaacagcgc accgattacg cgcctttgaa
     6361 tggctaaaga ttcccaaaat gatgttttat gcaaggcttt catcagtttt tatgctcacg
     6421 aatgctggca gtcaacaggc tcaaaatacc ataaaccatc aagccgataa tcagagtggc
     6481 aacaatgttg tataaccgtt taggctcatg tgccaaatcc ggcaggctcg gttgcgagat
     6541 cacttccaaa taaagctgct gacggtctgc ttcaaccttg gcactttcca aagaagtcat
     6601 ggcggctgcc aactgctgct ctgccaactg gttttccaaa tacacacgct gatattcggc
     6661 agcctgatta gacaacgaag aatgcccacc gcccgaaatg gcacgtaact gttggtcaat
     6721 ttctttacgc aagctctgct cacgcgcctg caaacccgga atctgcggat tctccggagt
     6781 gactgctttc acctgatcca gctgggtttg aatcacaatc aattcatctt gcagcttgga
     6841 aaccaacccc atttgcactt ccgattgcgc tttcaaatca aaaacgccat tggcaatccg
     6901 gtaatccgtc agattctgag aggcttcctt tacccgctct gccgccgttt tcactacttc
     6961 ttccgcatag cgcaccgtat cagcacgtgc acgatcgttc aactggttaa tcaatgcttc
     7021 accttgtttt aacaaagcct cattgatttt cttagattcc agcgcatcaa aggaagttac
     7081 attcaacgtg gaaatacccg aaaccgtatc aaaattgatc atcacctgat ttttatagta
     7141 ttgataaaaa gcctcttcct cgccacggaa cccaaaccca ttaaagcggc tgaacgcatc
     7201 acctttggtt tcataaaact cacgcaccgg caagatttta cgcagttcat ccaaagacga
     7261 gcgcgaacgc atatactccc caaccgtgta aatatcatct tgcgcacggg caaaacctgt
     7321 gccctgcaaa atggcaccca ggccattgag agaagattgg cttttaggcg agcgcaccac
     7381 aaagctcgat tgcgacgtaa aacgatcgga agcgaagaag ccgaaataca ccaacgaaat
     7441 taccgtaggg ataatcaccg ttacccaaaa taaagggctt agctttttaa tccaactttt
     7501 ctttttcggc tttttacgct cggctttggt ttcagtggca actgccacag gaagttgctc
     7561 agacatctta attactcaca ttaattagtt aaattattaa tactgttcgc gccactggta
     7621 accggcgaga acacaaacga caagaatttc tgcacttcag ccaacggcgc attcgacaca
     7681 tacaatacat ctttattctt cacaggaaag cgctgcatag aaaatagcga atgcgcatca
     7741 gccatattca cacgatatac cgttggaatc tctgcctcac tgccataacc ttgagcaatc
     7801 catttatcct gacgttctgc cggcaattcc accaatggcg tatagcggaa cacaaacaca
     7861 ccacgcgcat cagaacggcg atcttgcaaa ccgcccatac ggccaatggc ttcagaaagc
     7921 gataagcctc tggctgaaaa accgatttct tgtgttctcc ccaccgcacc catagacgta
     7981 aaggtatagg gattggtaat catggtaacc acatcaccgc gacgcagcaa aatattttgt
     8041 cgcggatttg caactaaatc ttccaaggca acagttcgta ctacattgcc acgtgtcagc
     8101 tgcacattcg tatcctgcac atttgccgtt gaaccaccta ccgcagccac cgcatccaac
     8161 acacgctcac cggctgccgt cagcggcata cgcacactat tcccagcacg aatcaccgac
     8221 acattcgccg cattattctg caccaaacgc accatcactt gtggctgatt ggccattttt
     8281 ttcaggcggc ctttaataat ttcctgaacc tgaccaggcg ttttaccgac caccgaaata
     8341 tcgccaacaa acggcacaga aaccgtacca cgtgccgtga ccaactgctc tggcaactta
     8401 gtttgatgcg cactacccga gcccatcgaa gaaaggccac caccaaacaa tactgccggc
     8461 ggcgcttccc aaatcataat atccaataca tcaccaatat ttagcgtacc agccgaagca
     8521 taaccatcgc caaactgagt gaatgactga tttatctgag ccttatataa taactgagca
     8581 accgtatgat tcacatcaat cagctccact tcaggaattt gaacttcaga ttgttgccct
     8641 aaagagacaa ttttttttgc gctggggcct gatgaaggaa tcgcagagca tcctacaatt
     8701 aaacttccac acaataataa tactgcgtga cgaatataaa atttcacttt aaacacaagc
     8761 caaatcctaa tataattata aatggcctaa ttatagcact taatcgaaat aaatttatga
     8821 gtacgtagag tataattagt attcttcttt ccaacttcct tatacttata gattctaaaa
     8881 tcatgaaaag aattctttgc attacaggta ccagagccga cttcggcaag ctaaaacctt
     8941 tattagccta tattgaaaat cacccagacc ttgaattgca tttgattgta actggtatgc
     9001 atatgatgaa aacatatggc agaacctaca aggaagtaac tcgagaaaac tatcaacata
     9061 catatctgtt ttcaaatcaa atccaaggtg aaccaatggg tgccgtttta ggcaatacca
     9121 ttacgtttat ctctcgtcta tctgatgaaa ttgaacctga tatggtcatg attcacggcg
     9181 accgtttaga agcactagca ggcgcaacta taggtgcatt aagcagccgt ttagtttgcc
     9241 atatcgaagg tggtgaacta tctggtacag tagatgactc cattcgtcat tctattagta
     9301 aactttctca tatccacttg gtagcaaatg aacaagctgt cactcgccta gtgcaaatgg
     9361 gagaaaaaag aaagcatatt cacatcatcg gctcccccga tttagatgtt atggcctctt
     9421 ccaccctccc atccttagaa gaagtcaaag aatattacgg tttaccatac gaaaattatg
     9481 gtatttctat gtttcacccc gtgactacag aagcacattt aatgccacaa tatgcggccc
     9541 aatatttcaa agcattagaa ttaagtggcc aaaatatcat tagcatctac cctaataatg
     9601 acactggcac tgaaagtatt ctgcaagaat tattaaaata tcaatctgat aaatttattg
     9661 catttccttc cattcgattt gaatattttt tagtcttatt gaaacatgct aaatttatgg
     9721 tcggcaactc aagtgcaggt attagagaag ctcctctcta cggtgtccct tcaattgatg
     9781 ttggtacacg ccaaagtaac cgccatatgg gaaaatctat tattcataca gattatgaaa
     9841 ctaaaaatat ctttgatgcg attcaacaag catgcagttt aggcaaattt gaagcagatg
     9901 atacctttaa tggcggagat actcgcacca gcacagaaag atttgctgaa gtaatcaaca
     9961 atcctgaaac gtggaatgtt tctgctcaaa aacgttttat cgatttgaat ctttaaatta
    10021 tggaaaaaca aaatattgcg gttatacttg cgcgccaaaa ctccaaagga ttgccattaa
    10081 aaaatctccg gaaaatgaat ggcatatcat tacttggtca tacaattaat gctgctatat
    10141 catcaaagtg ttttgaccgc ataattgttt cgactgatgg cgggttaatt gcagaagaag
    10201 ctaaaaattt cggtgtcgaa gtcgtcctac gccctgcaga gctggcctcc gatacagcca
    10261 gctctatttc aggtgtaata catgctttag aaacaattgg cagtaattcc ggcacagtaa
    10321 ccctattaca accaaccagt ccattacgca caggggctca tattcgtgaa gctttttctc
    10381 tatttgatga gaaaataaaa ggatccgttg tctctgcatg cccaatggag catcatccac
    10441 taaaaaccct gcttcaaatc aataatggcg aatatgcccc catgcgccat ctaagcgatt
    10501 tggagcagcc tcgccaacaa ttacctcagg catttaggcc taatggtgca atttacatta
    10561 atgatactgc ttcactaatt gcaaataatt gtttttttat cgccccaacc aaactttata
    10621 ttatgtctca tcaagactct atcgatattg atactgagct tgatttacaa caggcagaaa
    10681 acattcttaa tcacaaggaa agctaaatgc aaaacaacaa cgaatttaaa attggtaatc
    10741 gttcagtagg ttacaaccac gaaccattga ttatctgtga aatcggcatc aatcatgaag
    10801 gctctttaaa aacagctttt gaaatggttg atgctgccta taatgcaggc gctgaagttg
    10861 ttaaacatca aacacacatc gttgaagacg aaatgtctga tgaggccaaa caagtcattc
    10921 caggcaatgc agatgtctct atttatgaaa ttatggaacg ttgcgccctg aatgaagaag
    10981 atgagattaa attaaaagaa tacgtagaga gtaagggtat gatttttatc agtactcctt
    11041 tctctcgtgc agctgcttta cgattacaac gtatggatat tccagcatat aaaatcggct
    11101 ctggcgaatg taataactac ccattaatta aactggtggc ctcttttggt aagcctatta
    11161 ttctctctac cggcatgaat tctattgaaa gcatcaaaaa gtcggtagaa attattcgag
    11221 aagcaggggt accttatgct ttgcttcact gtaccaacat ctacccaacc ccttacgaag
    11281 atgttcgatt gggtggtatg aacgatttat ctgaagcctt tccagacgca atcattggcc
    11341 tgtctgacca taccttagat aactatgctt gcttaggagc agtagcttta ggcggttcga
    11401 ttttagagcg tcactttact gaccgcatgg atcgcccagg tccggatatt gtatgctcta
    11461 tgaatccgga tacttttaaa gagctcaagc aaggcgctca tgctttaaaa ttggcacgcg
    11521 gcggcaaaaa agacacgatt atcgcgggag aaaagccaac taaagatttc gcctttgcat
    11581 ctgtcgtagc agataaagac attaaaaaag gagaactgtt gtccggagat aacctatggg
    11641 ttaaacgccc aggcaatgga gacttcagcg tcaacgaata tgaaacatta tttggtaagg
    11701 tcgctgcttg caatattcgc aaaggtgctc aaatcaaaaa aactgatatt gaataaaaat
    11761 ctataaattg actcaattta atgataatcg gctgactttt cagtcgatta tcattaaaaa
    11821 tatacggaaa aacaaatgtt gcagaaaata agaaaagctc tcttccaccc aaaaaaattc
    11881 ttccaagatt cccagtggtt tgcaacacct ttatttagca gcttcgcacc caaaagcaat
    11941 ctttttatta tttctacatt tgcccaactt aatcaagctc atagtctgac taagatgcaa
    12001 aaattaaaaa ataatttact ggttatttta tataccacac aaaatatgaa aatgccgaaa
    12061 ttaattcaga aatcagttga taaagaatta ttttcagtaa cttacatgtt tgaactccca
    12121 agaaagccgg gaatcgtttc cccaaaaaaa ttcctatata tccagcgtgg atataagaag
    12181 cttctcaaaa ctatccaacc tgcacacctt tatgttatgt cttttgctgg gcattattcc
    12241 tctctgctca gcttagcaaa aaaaatgaat atcacaacac atttagtaga agagggaacc
    12301 gcaacctatg ccccactctt agaatcattt acatacaaac ccacaaaatt tgagcaacgt
    12361 tttgtgggta acaacttaca tcaaaaaggc tattttgata aatttgatat tttgcatgta
    12421 gctttccctg agtatgcgaa aaaaaatttc aatgctaatg aataccaccg tttttttgca
    12481 cattcaggcg ggattagcac aagccaatct attgctaaaa ttcaagacaa ataccgcata
    12541 tctcaaaatg actatatttt tgtaagtcaa cgctatcctg tttcagatga agtttattat
    12601 aaaactattg tcgaaacatt aaatcaaatg agtttgcgaa tagaaggtaa aatttttatt
    12661 aaactccatc ctaaagaaat ggagaataaa aatattatgt ctttattcct gaatatggta
    12721 acaattaatc cccgtctagt agtgattaat gaaccccctt ttctcattga cccactaatt
    12781 tatttaacaa cccctaaagg aattattgga ctgacatcga cttctattgt ttatacacct
    12841 ttactttccc ctactaccca atgtctgtca attgggcaaa tcgtgattga ttcgatacac
    12901 catacagccc aacaagagaa caccgcttta attgaagagc atttagagat tgttaaacaa
    12961 ttcgatttta ttaaaatatt atcaagtata gaggatggta tagacaccaa ttcttttaag
    13021 acagaagaaa cattagaaat gcttctaaaa tcagctgaat atgcatataa aaacaaaaac
    13081 ttttaccaag caatttttta ttggcaactg gccagtaata atgatctatc tgtattaggc
    13141 tataaatcat tatggtatta caatgccctt aataaagtta agcagaatta taaaatgaaa
    13201 tatttagaaa ttaactacat tgaaagaatt tccttatatt ttaatgataa agataaaatg
    13261 atttggcaaa acatcaaaaa tgattttttt aaatatagcc tttgtaacca ataaatatat
    13321 ttaagctgga gtcttattca tcatgtcaat caatacgttt gaaaccttta tttacgaatt
    13381 ataccaatca actaatttcg cagaaattgc tcgcattacg ggatttagta accataaagc
    13441 gctaaacttg gaaaccatcc gaatgatggc aattagctgc tttaaaacca aaaacaccac
    13501 aaaatgcatc gagctaagtg aatatttcaa tgaaaaatca gatattaaag atttttttat
    13561 ttttgaaatt ttggctgaat gctattttct gcaaaatgac cttgttaaat ctttagagaa
    13621 ctatgagaaa gcattattat taaatccaaa actaatctca gcaagattta aacatatgtg
    13681 tctcaaatat aggcttttta atgaactaga tagtactact ttctcaaaat atgaaattga
    13741 atctcaacaa aagaaaaaga tttcaaactt acgcattatt gcctatatcc aattaaaaga
    13801 aaaaaagtat tttaaggcat ataataacct gaaattactc atagagctaa acaaatctcc
    13861 tttctatccc gattatttat caataataca cgctattgaa aatttagagt attctaaaca
    13921 atcccaaaat actaaaaaag aactaacaga atatatcaaa atttccaaag caattgcact
    13981 aaaatcagaa ttcgtatgca acgggagtaa caatttattt gttactttat cacctgctac
    14041 aggtttcgtt cttaagaaat ataattaccc tgctgataaa ctatgcttta ttgacaacac
    14101 aaacacatat tatacttttg cttatgaatt gatcgcagag catattatta gcttagttaa
    14161 aaaatataaa tatgaaaata tttctattat tggctcatca aaaggaggaa ctgccacaat
    14221 tatacttcta aatttattgc agactgcatt acctaatact acaatatgcg cagtatcttg
    14281 ctctcctcaa atacagattt ttccattcaa taagaaccta actattccta gttatcaaaa
    14341 atttgcagaa tatttctctt ataactcaat tctagaaagc aaatgtgctc aagctcaaaa
    14401 gttaataaat tttgatattc tctatagaaa taaactaact attttttatg gtgataaatt
    14461 taaaatggac gcccaagaag tatctacaat tagacctata aacaatacta ctattattcc
    14521 tttaaactat tcaggacatg gttctcttat cccattaaca attcctgaaa ataaaagttt
    14581 tgacgaatta aagcagaaat attctaaact tgaaatcgac actgatttcc aagccttagg
    14641 tggcaataac ctttcaagta ttgtggatga gatttttgaa atctattcca atcctgatat
    14701 gcgattacat aaattcttat gttaaatcca atcaaaaaag cgtgaaggaa ccatcttcac
    14761 gctttttttt caaactcaac tatttcttct tctaaaccat ttagagtcat aaaaaaattg
    14821 cttgggatct ctaatgagct ttttaaactt ccgcaaaaaa atggtttggg ttgattctat
    14881 actgttcact gcaaataaat attcttggta atcatgttgc aatggcacaa cctgctttaa
    14941 tagcttatta aactcatcag ttgtaatcac ctttttagta gcatagcaat caagccagcc
    15001 cggatttgct atttttgggg aagtgaaatc tatttgatca aaataaatta aaaccccact
    15061 acctaaaaaa tgctgatttg cataggattt agaaaagcct aagtctgcaa caacaccaac
    15121 agggataccc ttacattcag cttcaaaaag catagtagat gaataagata aacaataccc
    15181 catttcttgg aaggcttgcg cggtactttt tcgctctatt gtcaaattac tcggcaactg
    15241 aaactgcttt gccagctcta tatacgaatg tttatcctga tgcacagtaa tatctttatc
    15301 tgcaaccctt agcaaaatag taaattcttt ctcagggtat ttttcagcca aggcaatcaa
    15361 tttttttaat gtgtaaattc tttcttcttt tttaaatgga attttgactt ggtcaataaa
    15421 ataaattctc tctccatgac aacctttcga agcatggcga aaatttggat aaccataaag
    15481 tatattttga caacttaatt tatattgttt tttatatgtc tctgcaattc taaaatcatg
    15541 cttattatta tataaaacaa tatcagcccc catacgagat agaatacttg cctgatcacc
    15601 gaatactaca cctggaaaca aggtaataat caatggcctt gaagcaatat ttaattgcgc
    15661 attctgctta aagaaccttt ttaacaaccc atttccaacc gataaaatta ctgcgtcata
    15721 atattgatgc atattcttaa taaagtaatc atcaatatga aaaaaaattg cttggtcttt
    15781 atctatccct gattctaata actgtcgatt tgaaagaatg ttttctcgat ttttgtggat
    15841 ataaatatca atttgagcat cttttatctc ttttgcaaca gcatagcccg agttaaggaa
    15901 cgagtcataa ctggcaatta gtaatacttt tttcactatt ttcccttaat tctacaccat
    15961 atgaataaaa ttatagtatt ttaccttaga tatcaaatat acttaaccaa aaaaatctga
    16021 agaaatctac tgctacttca gggataatta tttcattaag attttacaac cattattatc
    16081 aatctatcaa agtattttta gtattttatt ctatgaaaaa aattctcgtt accggcggca
    16141 ccggttttat cggctcgcat accgttgttt ctttgctgaa aagcggccat caagtcgtga
    16201 ttttggataa cctatgcaat tccagcatca atatcctgcc acgcttgaaa acgattaccg
    16261 gccaagagat tccgttttat caaggcgata tccgcgatcg tgagattttg cgccgtattt
    16321 ttgcggaaaa ccgcattgat tcggtgattc attttgccgg cttgaaagcg gtgggtgaaa
    16381 gtgtggccga gccgatgaaa tattatgata ataatgtttc cggcagcttg gtgttggcgg
    16441 aagaaatggc gcgtgcgggc gtgtttagca ttgtgttcag ttcttcggcg acggtttatg
    16501 gcgatccggg caaagtgcct tataccgagg atatgccact gggcgatacc accagccctt
    16561 acggcacatc gaaatcaatg gttgagcgca ttctcactga tattcaaaaa gccgatccgc
    16621 gctggagcgt gattttgttg cgttatttca atccgattgg cgcgcacgaa agtggtttga
    16681 ttggcgagca gccaaacggt atcccaaaca atctgctgcc ttatatctgc caagtggctt
    16741 cgggcaggct gccgcaactg tcagtattcg gcggcgatta tccgaccccc gacggtacgg
    16801 gaatgcgcga ctatattcat gtgatggatt tggcagaagg ccatgtcgcg gctatgcagg
    16861 cgaaaagcaa tgtagcaggc acgcatttgt tgaacttagg ttccggccgc gcttcttcgg
    16921 tattggaaat catccgcgca tttgaagcag cttcgggttt gacgattccg tatgaagtca
    16981 aaccgcgccg tgccggtgat ttggcgtgct tctatgccga cccttcctat gcaaaggcgc
    17041 aaatcggctg gcaaacccag cgtgatttaa cccaaatgat ggaagactca tggcgctggg
    17101 tgagtaataa tccgaatggc tacgacgatt aagttgacct gatacaggcc gtctgaaaga
    17161 gatgttttca gacggcctct ttatctgaaa aacacacatt ctgtctgcta taatctgttt
    17221 atattttttg gctatcctct gaaatttatg agaaaaatcc ttgttaccgg cggcgcgggc
    17281 tttatcggtt ctgccgttgt ccgtcatatt atccgaaaca cccaagattc cgtcgtcaac
    17341 ctcgacaaac tgacctacgc gggcaatctg gaatcgctgg aacctgtgtc ccatcatccg
    17401 cgctatgcgt ttgagcaagt cgatatttgc gaccgcgcag cactggatcg tgtgtttgcc
    17461 caacaccagc cggatgcggt gatgcacttg gcagcagaaa gccatgtcga ccgcagtatt
    17521 gatgggccgg cggcatttat cgaaaccaat attgttggta cttacacgct tttggaagcg
    17581 gcgcgtgctt attggcatga tatgccgtct gaaaaaaaag cggctttccg tttccatcat
    17641 atttcgactg atgaagtgta tggcgattta agcggcacag acgatttgtt taccgaaacc
    17701 acgccctacg cgccgtccag cccctactct gcctctaaag cgtccagcga ccacctcgtc
    17761 cgcgcgtggc agcggactta cgg
//
LOCUS       DAWKST010000025        17329 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.025, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000025 DAWKST010000000
VERSION     DAWKST010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17329
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.025"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..174)
                     /locus_tag="WJL87_000597"
     CDS             complement(<1..174)
                     /locus_tag="WJL87_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799064.1"
                     /translation="MPSERLSDGIFPCVLNRIHETPDAFGGNVGCRAMFGGDQRTKYA
                     GKGLMPSEGSDGIG"
     gene            complement(192..749)
                     /locus_tag="WJL87_000598"
     CDS             complement(192..749)
                     /locus_tag="WJL87_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ7799065.1"
                     /translation="MDALKLLTNRRSSKKLKPPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFIVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMSPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDVEAFVSHW"
     gene            complement(825..2459)
                     /locus_tag="WJL87_000599"
     CDS             complement(825..2459)
                     /locus_tag="WJL87_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HEZ7799066.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPVGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2533..2862)
                     /locus_tag="WJL87_000600"
     CDS             complement(2533..2862)
                     /locus_tag="WJL87_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799067.1"
                     /translation="MSRCCLKRLQTASAVSAFSGGVLEVVRLCCRRLSVLFNPGVMLF
                     VPDCPVGGAAGFFQYEMLCPFFWQGRLQTGSNLAYDVFMSAGRLNGGRNPPHSRRFLA
                     LLCSVAL"
     gene            complement(2862..3968)
                     /gene="serC"
                     /locus_tag="WJL87_000601"
     CDS             complement(2862..3968)
                     /gene="serC"
                     /locus_tag="WJL87_000601"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ7799068.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNREGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KTVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4333..4764
                     /gene="rimP"
                     /locus_tag="WJL87_000602"
     CDS             4333..4764
                     /gene="rimP"
                     /locus_tag="WJL87_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ7799069.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            4792..6294
                     /gene="nusA"
                     /locus_tag="WJL87_000603"
     CDS             4792..6294
                     /gene="nusA"
                     /locus_tag="WJL87_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ7799070.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVDENQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6306..9194
                     /gene="infB"
                     /locus_tag="WJL87_000604"
     CDS             6306..9194
                     /gene="infB"
                     /locus_tag="WJL87_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ7799071.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKTGGGDSLTLDDKQ
                     LLNAYLTKKNGSNSSTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSKKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRTKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPM
                     VVAVNKIDKEAANPERIRQELTAHEVVPDEWGGDVQFIDVSAKKGLNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMLKGYNEIMEGDQLECFDIVEVARSL"
     gene            complement(9406..10800)
                     /locus_tag="WJL87_000605"
     CDS             complement(9406..10800)
                     /locus_tag="WJL87_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HEZ7799072.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQLPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSGKYSEL
                     NLNQDKAASRRQYK"
     gene            complement(10874..12214)
                     /locus_tag="WJL87_000606"
     CDS             complement(10874..12214)
                     /locus_tag="WJL87_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ7799073.1"
                     /translation="MLNPSRKLVELIRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRDTLERVRAGSFWLWVAAATFAFFTGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLGDSSSVRLVEMLAWLPA
                     KLGFPVPDARAIIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWAVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALAECGAAWLEPDRAAQEGRLKDQ"
     gene            12357..12983
                     /locus_tag="WJL87_000607"
     CDS             12357..12983
                     /locus_tag="WJL87_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ7799074.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLALMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYVVMGWMVLAVMKSLTASLPLAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(13047..14465)
                     /locus_tag="WJL87_000608"
     CDS             complement(13047..14465)
                     /locus_tag="WJL87_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ7799075.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDYIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKG"
     gene            14792..15520
                     /locus_tag="WJL87_000609"
     CDS             14792..15520
                     /locus_tag="WJL87_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ7799076.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(15579..16067)
                     /locus_tag="WJL87_000610"
     CDS             complement(15579..16067)
                     /locus_tag="WJL87_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ7799077.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRRRAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWSLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(16825..17289)
                     /locus_tag="WJL87_000611"
     CDS             complement(16825..17289)
                     /locus_tag="WJL87_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEZ7799078.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
BASE COUNT         4567 a         4829 c         4355 g         3578 t
ORIGIN      
        1 accgatgccg tctgaacctt cagacggcat gagacctttg cctgcgtact tggtgcgctg
       61 gtcgcctccg aacatggcgc gacacccgac atttccgccg aacgcatcgg gcgtttcatg
      121 aatccggttt aaaacgcatg gaaaaatgcc gtctgaaagc ctttcagacg gcattttgct
      181 tgagattccg tttaccaatg actgacaaac gcttccacat cggtattctt gggcttatgc
      241 acttcctctg tcggcgtgcc gaccatcatc agcccgatga ttttatcctt atccgcacat
      301 ccgaaagcct cacgcaacag ggggctgttg acccacatcc ccgtaatcca gacattgtcg
      361 aaaccttgcg ccgttgccgc cagttgcagc gcatacgccg cacaacccgc cgtcagcatc
      421 tgctcccatt ccggtttcgg cttaggcaca tcgcggttcg gcgcaaacgt taccccgata
      481 accatcggcg acatattgcc cactttttcc gcctttttca tcgcatcgtc gccgaaattc
      541 aattcggcaa ccgtttgctt taacacatcg cgaaaacgtt gcaatcctac ctcgccttga
      601 atcacgataa aacggaaggg gcgcatattg ccgtgatcgg gaacttgggt tgccgcctga
      661 aatatttgtt ccaactccgc cgcatcgggg gcggggggct tcagcttttt ggaagatcgg
      721 cggttcgtca ataattttaa agcatccata tcgttatact ccggtcatcg gtcgggtgtt
      781 cggacacata tgccgtccga aggcttcaga cggcatatcc ggcatcagcg cggacggcgg
      841 caggctgcca atatatccat ttccttccga taggtttgac tgttggaaat gtccatcagc
      901 cccaataccg tgctgaaata gtggtcgtgc gaatattcgt tttccgccgc tttttgtttg
      961 aggcattgga aatctatgcc cccgtgttgg cggaaggctt tggaaaacca cataaccatc
     1021 gggatatgcg tctgcccgga aggcgcgatg gcgtaaggcg cggcgtgcag gtacatcccg
     1081 ttttcgccca aactttcgcc gtggtcggaa acataatgca ccacgctttc caaatcgtcg
     1141 cggttttcaa gtttgcggat aaccttgtcg ataaactggt ccacatacaa aaccgtattg
     1201 tcgtaagtgt tgaccagcgt ggcgcgggtg catttgttga tttcgttggt gtcgcaggtc
     1261 ggcgtgaatt tgcgttcggc ttcggtatag cgttcgtaat acgtcggccc gtggctgccg
     1321 atggtatgca ggattaaaac cgcgtcttta tcgtttttgt tgaggacttc gtcgaactta
     1381 gtcagcagga tattgtcgag gcactcgccg ttgcggcagt attcgggcag gttgagcgag
     1441 gtaacgtcgg tattcggcac tttgccgcac acgcccttgc agccggaatc gttttccaac
     1501 caagtaactt ccacgccggc gcgctgcacg atgtccagca ggttgtcttg gtgttcggct
     1561 ttgatttcgt cataatccgt gcggtcgaag gttgagaaca tacacggcag ggagtgcgcg
     1621 gtcgatgtgc cgcagcttct gacctgcggg aaattgacaa tttcatcgcc gcgcgcggca
     1681 agcagcggcg tagtttggcg gctgtaaccg ttcaaacccc agttggcggc acgcgtggtc
     1741 tcgcccacga ccagcaccac gaaacggcgc aggctgccga ccgggcggtt ttgcacgacc
     1801 gccatatcca attgcgtata aggaatattg gaacgcttcc aatctttgta tttcgacacg
     1861 cccgcgccga tgaaattaga cggcacaatc agatgggtta ctgatttatt gttgcggaaa
     1921 aacgaggcgt aatcctgata ttgcaacatt gcgatgccca acgcgcacaa aaaggaaacg
     1981 gcggcaagca caaggcgcgt caaaagctcc ttataccaaa cgcggtattt aaccttgacg
     2041 gcgatatacg ccagcgcggg caatacgccc aaacatacaa tccacagcac atagcccggc
     2101 gtaatcaggc gcgcgctttc cgccgccgtg gtttgcaaga cattattcaa catcgacttg
     2161 ttgaaataga tattgaaaaa tatttcttgg taagacaccg ccgcactgat aaccaatatc
     2221 aacggaatca ataccttatg cacgaaaggc aaggcaatga cgtgaaaaac gaaattactt
     2281 aaaaaaaaca gcaccaccgg catcgtatag aggaagatat ccgccccggt gccgttaaaa
     2341 ggatgaagct cgacaacttt ggcaaaaaag gcgtaattca ataccagcga ggaatacagg
     2401 gaaaggaagg caatcagcgc ggaagagccg agcttcggcc tcaggttcgg ttttatcatt
     2461 tggaatgtgt cggataaggg ttggaaaagg catccggcat ttggaatccg gattattgaa
     2521 aaagattctt aattataagg caacggagca aagcagggca agaaaacggc ggctgtgcgg
     2581 gggattccgc ccgccattca aacgtccggc agacataaaa acatcgtaag caagattcga
     2641 accggtctgc aaccgcccct gccagaaaaa cgggcaaagc atttcatatt ggaaaaaccc
     2701 ggccgcgccg ccgacgggac agtccggcac aaacagcatc acgcccggat tgaaaaggac
     2761 ggatagccgc cggcagcaca atcttaccac ctccaaaacg ccgccggaga acgccgaaac
     2821 agccgatgcc gtctgaagcc gcttcagaca acatcgggac atcaaccgta acgccgttgg
     2881 aaatcgcgca taaaatccgc caaagcccgc acgccttcaa gcggcatcgc attataaatg
     2941 ctggcacgca taccgccgac ggttttatag cccttaagca ggcacaagcc ctgcaattcg
     3001 gcttccagca caaaacggcg gtcaagctcc tcatcccccg tttggaacac gacattcatt
     3061 ttagaacgcg cattcggacg gatacggttg atatagaaac cgccgctgcc gtctatcgtc
     3121 tcatacaagg tttgcgcctt cagccgattg accgcttcaa tttttttcac accgccctgc
     3181 gcctgcagcc agcggaacac cagccccgac atataaatcg cgtaagttga cggcgtgttg
     3241 tacatacctt cgcggttcaa atgcgaacgg tagttgaaca catcgggaat atcgttcgga
     3301 caacgctcga gcaaatcctc acgcacaatc accaccgtaa cccctgccgg cccgatgttt
     3361 ttctgtgcgc ctgcgtaaat cagtccgtag tcggcaacat caaactcgcg cgacaaaatc
     3421 tcgctggaca tatcgcacac cagcggcggc atgccttctg aaaggcgcgg cacttcacgg
     3481 tattgcagcc cgttgaccgt ttcattgacg gcaaaatgga caaacgccga atcgggtgca
     3541 acatcccacg tttccacagg cggcaggtcg agatagtcga actgctcgcc gccatgcgcc
     3601 gccaaacgga tttccgtatc ggtcaaacgg ctcatctgtt cataagcgat acggctccag
     3661 ttgcccgtta ccaccgcgtc ggcagtgcgg aaaccgtgtg ccagattcat ggctgccata
     3721 ttaaattggg ttgttgctcc gccctgcaga aacaatatct tatagttgtc aggcactttc
     3781 aaaagctgcc tcaaatcctg ttccgcatga tgcaggatgc tcaaaaacat ttccgaacgg
     3841 tggctcattg ccatcacagg aaaacccgta ccgttgtagt ccaacatttc ctgccgcgcc
     3901 gtttccaaca cggcttcggg caatacggca gggccggcgg aaaaattgta aatcggataa
     3961 agagacatga tgcagccttg attctgaaca ataacccgcc cgattttagg cttgcagcag
     4021 gctttgtgca aggatggaaa atacctgtcc tccgccccgc ttcatgccgc ccgaacacag
     4081 aaaacaacat caatatattg atttacaaac ataaaaatca tgcacgcgac aaatagatac
     4141 atttgttttg tcaacaatat tcacgatttc ccattacaaa cctcccttac atccgctttt
     4201 ttccgtccca aaaacacaaa ataaatcaac actttcattt ctccgcaaaa gcggttataa
     4261 ttacgccgat ttttcaactt tgacgaaaaa tggccacggg gccatttttt tattatgtac
     4321 atagggagca gcatggatat ccaaaccatc ctcgaaaaaa ccctgcccgg cctgggctac
     4381 gaactggtcg attttgaact gaccgcgcaa ggaacattgc gcgtgttcat cgacaaagaa
     4441 ggcggcatta ccgtcgaaga ctgcgcaacc gtcagcaacc acttgagccg cgtcttcatg
     4501 gttgaagaca tcgactacaa aaacctggaa atttccagcc ccggactcga ccgccccttg
     4561 aaaaaagccg ccgacttcgt gcgctttgcc ggtcagaatg ccaaaatcaa aacccgcctg
     4621 ccgatagacg gtcaaaaaaa ctttatcggt aaaatcgaag gctgcgaaaa cgacactgtt
     4681 accgtttcct tcgacggcaa aaccgtacaa atcgaattgg gcaacatcga caaagcccgc
     4741 ctgcgccccg aattcaaatt ctaaaacaca acaatattgg agatgttcaa aatgagtcgt
     4801 gaaatgttac agctggcaga agcactggca agcgaaaaaa acgttgatgc ggaagtcgtc
     4861 ttccaagcac tggaattcgc cctgtctacc gccgccaaga aaaaggcaga ccgcgaacac
     4921 atggacgtgc gcgtccaaat caaccgcgac accggcgaat accaaacctt ccgccgctgg
     4981 ctgattgtcg ccgatgaaga ctatacctat cccgatgtcg aaaaaaccat cgaggaaatc
     5041 caagaggaaa ttcccggcac taccatccaa atcggcgaat actacgaaga gcagctaccc
     5101 aacgaaggct tcggccgcca agccgcgcaa accgccaaac aaatcatcct gcaacgcatc
     5161 cgcgatgcgg agcgcgagca aaatctgaac gagtttctcg ccgtcaaaga agacatcgtg
     5221 tccggcacgg tcaaacgcgt cgaacgccac ggcatcatcg tcgaagtcgt tgccggcaaa
     5281 ctggacgcgc tgattccgcg cgaccaaatg attccgcgcg aaaacttccg cagcggcgac
     5341 cgcatccgcg ccctcttcct gcgcgtcgaa gaaatcggca acaccggccg caaacaagtc
     5401 atcctgagcc gtacttccgg cgatttcctc gtcaaactgt acgccaatga agtacctgaa
     5461 attgcagacg gcatgcttga aatccgcgct gtcgcccgcg acccgggcca acgtgccaaa
     5521 gtcgccgtca aagccaacga ccagcgcatc gatccgcaag gcacctgtat cggcgttcgc
     5581 ggttcgcgtg tcaatgccgt cagcaatgaa ttatccggcg agcgcatcga cgttgtcctt
     5641 tggtcgcccg agcctgccca attcgtcatg agcgcgctct cacccgccga agtcagccgc
     5701 atcgtgattg acgaagacaa acacgccgtt gacgtgattg ttgatgaaaa ccaactggcg
     5761 ctagccatcg gacgcggcgg tcaaaacgtg cgccttgctt ccgacctgac cggctggcag
     5821 ctcaacatca tgacttccgc cgaggcagac gaacgcaatg cggcagaaga tgccgccatc
     5881 cgccgcctgt ttatggatca cttgaacgtg gacgaagaaa ccgccgacgt actggttcaa
     5941 gaaggttttg caaccttgga agaagtcgcc tatgttcctg ccgccgaact gcttgccatt
     6001 gaaggatttg acgaagaaat cgtcgatatg ctccgcaacc gcgcccgcga tgccatcctg
     6061 accatggcga ttgccgccga agaaaaactg ggcgaagtat ccgacgatat gcgcaacctc
     6121 gaaggcatag atgccgatat gctccgcagc cttgccgaag caggcattac cacccgcgac
     6181 gacttggcag agcttgctgt ggacgaactg attgaaatca ccggtgtaaa cgaagaaacc
     6241 gcaaaagccg tcatcctgac cgcacgcgaa cactggttta ccgaagacaa ataaaggggg
     6301 tacagatgag taacacaacc gtagaacaat ttgccgccga gctgaaacgc cccgtcgaag
     6361 acctgttgaa acagttgaaa gaagccggcg tcagcaaaac cggcggcggc gattccctga
     6421 cgctggacga caaacagctt ctgaacgcct acctgaccaa gaaaaacggc agcaacagca
     6481 gcaccatcag catccgccgc accaaaaccg aagtcagcac cgttgacggc gtaaaagtcg
     6541 aaacacgcaa acgcggacgc actgtcaaca ttccttctgc cgaagagttg gcagcacagg
     6601 taaaagccgc ccaaacccaa gccgcacctg tccggccgga gcagacggca gaagacgcgg
     6661 caaaagcccg agccgaagct gccgcacgtg cagaagcccg tgccaaggca gaagcggaag
     6721 cggcaaaact gaaagcggca aaagcaggca acaaagccaa acctgccgcg cagaaaccca
     6781 ccgaagcaaa agccgaaacc gcacccgttg cggcggaaac caaacccgcc gaagaaagca
     6841 aagcggaaaa agcccaagcc gacaaaatgc cgtctaaaaa acccgccgag cccaaagaaa
     6901 aagccgccaa gccgaaacac gagcgaaacg gcaaaggcaa agatgccaaa aaaccggcga
     6961 aacctgccgc acctgccgtg ccgcaacccg tggtcagcgc ggaagaacag gcgcaacgcg
     7021 atgaagaagc acgccgtgcc gccgcacttc gcgcccacca ggaagccctg ttgaaagaga
     7081 aacaggaacg ccaggcacgc cgcgaagcca tgaaacaaca ggcagaacaa caggcaaaag
     7141 ccgcacagga agccaaaacc ggcagacagc gtcccgccaa acctgccgag aaaccgcagg
     7201 cagccgcgcc ggccgtcgaa aacaaacctg tcaatccggc aaaagcgaaa aaagaagacc
     7261 gccgcaaccg cgatgacgaa ggtcaaggcc gcaacgccaa aggcaaaggc ggaaaaggcg
     7321 gacgcgaccg caacaatgca cgcaacggcg acgacgagcg cgtacgcggc ggtaaaaaag
     7381 gcaaaaaact caaactcgag ccgaaccaac acgccttcca agcaccgacc gaacctgtcg
     7441 tacacgaagt tttggtgcct gaaaccatta ccgttgccga cttggcacac aaaatggcag
     7501 tcaaaggcgt ggaagtggtc aaagccctga tgaagatggg catgatggtt accatcaacc
     7561 aatccatcga ccaagacacc gccctgattg tcgtcgaaga actcggccac atcggcaaac
     7621 ccgccgccgc cgacgatccc gaagccttct tggacgaggg cgcggaagcg gtggaagccg
     7681 aagcattgcc gcgtccgccc gtcgttaccg tcatgggcca cgtcgaccac ggcaaaacct
     7741 cgctgctgga ctacatccgc cgtaccaaag tggtacaggg cgaagcaggc ggcattaccc
     7801 aacacatcgg tgcataccac gttgaaaccc cacgcggcgt gattaccttc ttggatactc
     7861 cgggtcacga agcgtttact gccatgcgtg cgcgcggtgc gaaagcaacc gatatcgtga
     7921 ttcttgtggt cgccgccgac gacggtgtga tgccgcaaac catcgaagcg attgcccacg
     7981 ccaaagcggc aggcgtaccg atggtggttg ccgtcaacaa aatcgataaa gaagccgcca
     8041 acccagagcg tatccgccaa gagctgaccg cacacgaagt cgtgcctgac gaatggggcg
     8101 gcgatgtaca gtttatcgac gtttccgcta aaaaaggcct gaacatcgat gcattgctcg
     8161 aggcagtctt gctcgaagcc gaagttttgg aactgaccgc acctgtcgat gcgcccgcca
     8221 aaggcatcat cgtcgaggcg cgcttggaca aaggccgcgg cgcggttgcc acattgctgg
     8281 ttcaaagcgg cacgctgaaa aaaggcgata tgctgctggc cggtacggca ttcggcaaaa
     8341 tccgcgcgat ggtcgatgaa aacggcaaat ccattaccga agccggtccg tccatccccg
     8401 tcgaaatcct cggcttgtcc gacgttccga acgcaggcga agatgcaatg gtattggctg
     8461 atgagaaaaa agcgcgcgaa atcgccctct tccgtcaagg caaataccgc gatgtgcgcc
     8521 ttgccaaaca acaggcggcg aagctggaaa atatgttcaa caatatgggc gaaacccagg
     8581 cccaatcttt gtcggtcatc atcaaggcag acgtgcaagg ttcttacgag gctttggcgg
     8641 gcagtctgaa aaaactgtcc acagacgaag tgaaagtgaa cgtgttgcac agcggcgtgg
     8701 gcggcattac cgaatcggat gtcaaccttg ccatcgcttc gggcgcgttc attatcggct
     8761 ttaacgtgcg tgcagatgcc tcttcgcgca aacttgccga aaatgaaaat gtggaaatcc
     8821 gctactacaa catcatctat gatgccatcg acgacgtgaa agccgccatg agcggtatgc
     8881 tttccccgga agagaaagaa caggttaccg gtacggtcga aatccgtcag gtcatctccg
     8941 tttccaaagt cggcaacatt gcaggctgta tggttaccga tggtgtggtc aaacgcgatt
     9001 cccatgtccg cctcatccgc aacaacgtgg tcatccacac gggcgaactg gcttcgttga
     9061 aacgctataa agacgacgta aaagaagtcc gcatgggctt tgagtgcggt ctgatgctca
     9121 aaggctacaa cgaaatcatg gaaggcgacc aactggaatg cttcgacatc gtcgaagttg
     9181 cccgcagcct gtaattcctt tgcaaataaa atgccgtctg aagcgttcag acggcatacg
     9241 aaacgggttc tgtatgatac agaacccgtt ttttgtcgca aatcggcttc agacagccct
     9301 cttgccttat cccgatttga atctgacttg ccatacaaac aggcttcaga cggcattata
     9361 gtgaattaaa tttaaaccgg tacagcgttg gctcgccttg ccgtactatt tgtactgtct
     9421 gcggctcgcc gccttgtcct gatttaaatt taattcacta tattttcccg acaggcggat
     9481 ttgaagtttc tccacatatc cggcgcgttc ggcgcggctg gtttcggggc ggtgcgtatt
     9541 gagcgacgac catttccaat gactgcgggc tttgttgagt tcgggcggga gtctggcggc
     9601 gtcccacggg gctttgcggc tgtgcaactc gatatccgat tgtgccgcgt gtccgcgcgt
     9661 ttgcaggacg tggagcagat cgagggcgcg ggcggcgagc agggtcaggg tttcagggtc
     9721 ggtgtgcaag gtttggcggc cggcgagttt gtcggaaatg gtgcgggtat tgggcaggat
     9781 gccgcccaaa aagccgccga ttgccgtacc caatccgagc gagccgccga gtgtggcgat
     9841 gtccagcccc aagccgatga gtgcgccggt tgccgcgccc gtgccggtgc ggatgccgta
     9901 ttgtttgagc aattcgctgt cgaacgggtc ttggcggaag gcttgcggca tccagtcgcc
     9961 gccgtcgatt tcgctgtggt agaaacggta gagggcaaac agccgctgct gcatctgccg
    10021 ttcgagttgg cgtatttccg cctgcatggt ttgtagcacg gtggcggtat cctcgttttc
    10081 gtccacttcc tgcctgaagg cggcggcatc gagcaggaaa tcggcgattt cgcggcgcgc
    10141 ttcgccgtcc agccgctgcc attcgcgccg gcgcatggct gtcaggcggt caagtgtgct
    10201 gcgttcgggc aacatggtgg cgaggttttc ccacaggcgt agttcgcctt caaaatcaaa
    10261 ggcgacggtg tcgaaccctg cgaaaacgtg caggtttctc ctcgccagca tggttgtcca
    10321 cgattcggga agctgtccgc cggtaaagtt gaacacgggc ataactggtt tggcacacca
    10381 tgaaaggatg gtcaattcat cccgatattt gtcgaggacg ggctcgcgcg cgtcgatgac
    10441 gtacattgcc atatcgcttt gcaagacttg ccgtaagact ttggcttcct gattgaaatc
    10501 atggtgcgcg ccgtggctgc cgagaaactg ttgcagccgt tcgatgccgt ctgaacgatt
    10561 gtccgtatgg ttttccagcc attccagcac gccgcccgcg tcttcaagtc cgggcgtgtc
    10621 gtacaggaaa accagcgtgt ctgcgccgtc gctgatggcg gcttcttcga catggcgtgt
    10681 ggtcgatggg gcgtttctga cttcgccgaa accgctgtcg cgcaacaggg tacgaaggag
    10741 cgaggttttg ccggtattgg tatgcccgac gacggcgagg gaaaggggtt gtttgttcat
    10801 gatgtttttg aagaatggat tttcagacgg tcttttttca gaatggcggc ttaacagaac
    10861 atttcaagtg agtttattgg tctttcaaac gcccttcctg cgccgccctg tcaggctcaa
    10921 gccacgccgc gccgcattcg gccagcgcgt tacgccaatg ttccagcttt tccgaaaggt
    10981 cgtctgaaag cccctgttcc gccaaaagct gcaccaccgc gccgccctgc gccgcttccg
    11041 aaagtcggac gatctgccgc aacacgccgc ggtcgggcac agtttgggcg cgcacgccga
    11101 taagcagttg cgccggtttc tgcttcagct ctgtctccag cgcggcaacc tgttcccgat
    11161 tggcggcaac gcccttatcc agccattcct gcgccagcct gccctcgaac cattcgccgt
    11221 cctgccattc ggtctccagc atgaccgccc atttcggcgc atcgttcaag acgattttcg
    11281 gcgaaacggc ggacacggtt tcccgacgcg tatccgcatc ggtgattttg ttctgccagc
    11341 ggcggatgac cgcctgataa tagggttttt ccaaatccaa tccgttttcg cttgttttca
    11401 aaaggatttt gcataccgcc caagccaaga ggcgcggcag gatgccgtag caggcgatac
    11461 tgccgaccag cagccccgac caagcccgcg catcggcaat attgccgttc agacggcctt
    11521 cgatgatcgc ccgcgcatca ggcacgggaa aacccagttt cgcaggcagc catgccaaca
    11581 tttccaccag ccgtaccgaa gacgaatcgc ccaacagcgt gctttcccag ttgaacgtat
    11641 attgccgcac caaaagcagc aacaataccg acaccagcat tccgagcagc gtgcagagcc
    11701 acaggctgtg cgacgttgcg cctattttcc aacgtaccga aggttgccgc cactcgtccg
    11761 catacagccg caacaccgcc tgatttacag ggtctttgcc ccgaaaccac gtcgccggac
    11821 tgctgaaaaa acgccccact ttcacgcgca ggaacaacat tgccaaccat actgccagca
    11881 tcagcgtatt catgcccaac acgcccgcca aaaccaaaaa gaaattcaga ccctgattgt
    11941 ccattagaag ataagtgcct gaaaaaccgg taaaaaacgc aaacgtcgcc gccgccaccc
    12001 acaaccagaa cgaccccgca cgcacacgtt ccaatgtgtc ccgcagcata cggttcctgt
    12061 cgatcatctc cgcccgacgg atgatttttt cctccgtact gccgtccacg cggcgcaaag
    12121 cctccgtcgc ctgcacggga tcgccgctga aaataaaacc gccttcgtcc aaaatacgga
    12181 tcagctcaac cagttttcgg gatggattca acataaaatg ccgtctgaaa ataaaaaaca
    12241 gattttaaca cacgcatttt caagaatatt cacagtgtag gcaaagagta aatctcacac
    12301 agaagcaaaa gtatcggcgt aaactgactg cctctacttt cccgaaagat tgtgcgatgt
    12361 atacaggcga acgcttcaat acttacagcc atttgagcgg tttgattctg gcggcggcag
    12421 gtttggcgct gatgctgctg aaaaccatag gacacgggga cggctaccgt atcttcagcg
    12481 tatcggttta cggcatcagc cttcttctgc tctatttgag ttcctcgctg taccacggaa
    12541 ttgcagccgg aaaactgaaa agcattttga aaaaaaccga ccactgcatg atttatgtgc
    12601 tgattgccgg aagctacaca ccgtttgcac tggtttcttt gagaaacggg ccgggctgga
    12661 cggtattttc actgtcctgg ctgctggcgg ctgcaggaat cgcacaagaa ctcaccatcg
    12721 gacggaaaag cgaaaaacga ctgctgtcta ttgcgattta tgtcgtcatg ggttggatgg
    12781 tcttggcggt aatgaaatcc ctgacagcct cactcccgct ggcaggactg gcttggctgg
    12841 cggcaggcgg tatgctgtac agcgtcggca tttactggtt tgtaaacgat gaaaaaatcc
    12901 gacacgggca cggaatctgg catctgttcg tattgggcgg cagcatcacc caatttgtca
    12961 gtgtgtacgg ttatgtaatc tgaatgccgt ctgaaaagca aaacctcccg atcctgaaga
    13021 ttgggaggtt ttctgtttgc cggacatcag cccttgtcgt ggaactcgtg aaattcatac
    13081 tgataggaca aatcccgacc cgcttttttc tgtgccaaat aatcatcata aatggcacgg
    13141 atttccttac gcaacaaaaa cagggctatc aagttcggga taaccataaa gccgttgaac
    13201 atatcggaca ggctccaaac caaatcgact ttgccgagcg tacccaaaac gatggcaagc
    13261 agaaccaatg cgcgatagat gcccaagtgt cttcccctga aaagaaaacg gatgttggac
    13321 tcaccgaaat aataccagcc gataatggtc gtcaaggcga aaaaggtcag acacacggca
    13381 agcaattgcg aaccgaagcc cggaaatgcc ttgttaaagg caaactgggt aaccgccgcg
    13441 ccctgttcgc ccgaaaggtt ggcatcagtc agcaggataa tcaatgccgt agccgtacat
    13501 accaaaatcg tatcgataaa cacaccgaca aatgccgcca taccttgctg cacaggatgc
    13561 ttcacatcag cggttgcatg tgcatgcggg gtagaaccca tacctgcttc gttggaaaac
    13621 agaccgcgcg ccacgccgaa acgtatcgct tcacgcatac cgatacccgc agcaccgccc
    13681 aaaacggctt caggattgaa ggcggcggta aagatgtggt tgaacatcgg cacaatatag
    13741 tcggaaaatt caaacaggat aacgacggcg cacaaaatat aaacaaccgc cataaacggc
    13801 acgacaaact gggcaatatt ggcaatacgg ttcacgccgc caatcacaac catgcccgca
    13861 aggacggcaa gcacaatacc gactgccaaa gaaggcacat caaatgcaat ggtaacggca
    13921 gaagcaatgg agtttgcctg tgtcgcatta ccgataaagc ccaatgcgat aatcaacgca
    13981 atggagaaaa agcccgacag gaaacgcgcc gcgcccctgc cgattttcgg agtcagaccg
    14041 tgcgtaatat aaaacgccgg cccaccgatg tatttgccgt ggctgacgac gcggtatttc
    14101 tgcgccagca gtgcctccgc aaaaatcgtg gacatcccca aaacggcaga aacccacatc
    14161 caaaaaatcg cgcccggccc gcctgcggtg atggcggtcg ccacgccggc gacgttgccc
    14221 gtaccgattt gcgcggatat ggcaaccgcc aacgcctgaa actgcgataa agacttatcg
    14281 tctttatcgc ctttggcaaa caagccgccg aatacagatt tgaatcccgc acccagcttg
    14341 gtaatctgcg gcgcaccgag atacagcgta aaaaacaggc cgatacccaa aagcgcgtaa
    14401 atcagcaggt agtcccaaag gaaccgattg actgtaccca ccagaacaga caatatattt
    14461 tccataaaat aaaccttatc ttacaattaa aatgactgcc ttccaaaaga cattccaata
    14521 aggaaacacg gcgagcagac cgtatttgcc gcaacagatg ccttaaattg tcaacaatcg
    14581 gggagaagct gcgccatcat accgtaaaat atcgttaatt aaaacatttc tttattttta
    14641 agcggaaagc ggaggaaatc gctttcagac ggcatcgaca acggcacggc ataaaacagg
    14701 atattttggg tacttgcaac ttatgttaaa atgccgaccg taaaaaatct gacaaaaaca
    14761 gattaattat ttgaaataag aaaggaaatt tatggcaggt catagcaagt gggcgaatat
    14821 ccagcataaa aaagcccgtc aggatgccaa acgcggcaaa atcttcaccc gtttaatcaa
    14881 agaaatcacc gttgcggcgc gtatgggcgg cggcgatccc ggcgcaaatc cgcgcctgcg
    14941 cctggctttg gaaaaagcag ccgaaaacaa tatgcccaaa gacaatgtgc aacgcgccat
    15001 cgacaaaggc acgggcaact tggaaggcgt ggaatacatc gaattgcgct acgaaggcta
    15061 cggcatcggc ggcgcggctt tgatggtgga ctgcctgacc gacaacaaaa cccgcaccgt
    15121 tgcggacgta cgccacgcgt ttaccaaaaa cggcggcaac ttgggtaccg acggctgcgt
    15181 ggcgttcaac ttcgtgcatc agggctattt ggtattcgaa cccggcgttg acgaagacgc
    15241 gctgatggaa gcggctttgg aagccggtgc ggaagacgtg gttaccaacg acgacggttc
    15301 catcgaagtc attaccgcgc caaacgattg ggcgggcgta aaatccgctt tggaggcggc
    15361 aggttacaaa tccgttgacg gcgacgttac gatgcgcgcc caaaacgaaa ccgaactctc
    15421 cggcgacgat gccgtcaaaa tgcaaaaact gattgacgcg ctggaagact tggacgacgt
    15481 gcaagacgtt tacacttccg ccgtattgaa tttggactga tacgcagcac agcagacata
    15541 caataaaatg ccgtctgaac ctttcagacg gcattgattt atttagccct tgcccacgcc
    15601 caccaaaccg tcaggacaac cgccagaaaa tacgccgcgc tccaaacagg caaagcaacc
    15661 cctaacagat aatccggttc ggcacaattt ccgaacccgc gcacgacagg ctcgaaccaa
    15721 tcaaacaaag accagccctt caagcggaac gtccacggcg caccgcacga aggagcggtt
    15781 cccggcggca gcgactgcaa ccacaactga tatgccgcaa cagaaatacc cgtaacggcc
    15841 ggaatgctga taaagacagc accgaacaaa ccgcccgccc ttcttcttgg tctgcacatc
    15901 aggacaattg ccgtacacaa tgcggttgcc aaaacgcata accgctgact gatacacaaa
    15961 acgcaaggct ccatacccaa aacatactgt gccgccaaag aaccggcaaa tgcacagacc
    16021 gaaacggcaa acagcagcca aacggctttt ctaaataacg gggtcatttt ctcaacacac
    16081 caatcaaaat accgatatgc cgattttgct ggatatatcc cgagatggca agggacgaat
    16141 cgacaccggc gcaggtgcgg acgatttcgg ttttcccgat atacacaaga cctgacaagg
    16201 gacaaaacgg cgatttgccc gcacaatgcc cgcaaaaaaa tacatccgga ggatttgaat
    16261 ttcggcaaat ttagcgaatc aaaagtttat ttcaatgaaa ttatatgatt tttttgaata
    16321 agcggattga caggtttttg aaggaatttg ttaccggata gccatcgggc aagttttttg
    16381 caaaatttga atcggtcggg taaattttca aaaaataatt gacagcggat aagaaacggc
    16441 ggataattcc cgccgtcgag ttgcttgatg cagcttgatt tttctcctct atttctcctt
    16501 tgtagacttg gcacacattc aactggatgt gtgcattttt ttatctccgc tttttttgaa
    16561 atttaattac ttttattgtt tgaaattcca ttctttaaga aatttaacaa gaaaaacgct
    16621 tgctttttga ccggaaaagc tgcataattc gttccgttag ctggttcgag tagtcagtta
    16681 atagtttctc ctatatttct cctttgtaga cttggcacac attctattga atgtgtgcat
    16741 ttttttatct gaagcaacaa gcctctgtgc gtgatgttgt tatgtttcat ttaggtgtca
    16801 aaccgcatac ccggtctgaa atattcaatc caaatccaaa accggatttt ctttgacctc
    16861 ctccatcaca acataactcc tactctccga agcggcaggc agttgcaata ggatattgcc
    16921 tagcatatcc cgataggcag acatatcggg caaacgtact ttaatcagat agtcgtattc
    16981 gcccgacacc aagtggcatt ccataatttg cggaattttc agcacttctt ttttgaaatc
    17041 ttcgaaaata ttgcccgatt tggattgcag cttcagctcg acaaaaacca atagcggttt
    17101 gcccaacaga tggggattga ggtgggcgtg atagccggaa atataatgtt cccgctccaa
    17161 acggcgcacc ctctctgtaa cgggcgtggt ggacaagcct accttctcgg caagctccgt
    17221 catcgggatg cgggcattct gttggaggat cttaagaatg cggaaatcga ttttatctag
    17281 ttctttcatt tggattgcct tgtatttatt attgatttta acaaataga
//
LOCUS       DAWKST010000026        17004 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.026, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000026 DAWKST010000000
VERSION     DAWKST010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17004
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.026"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(65..1312)
                     /gene="fabF"
                     /locus_tag="WJL87_000612"
     CDS             complement(65..1312)
                     /gene="fabF"
                     /locus_tag="WJL87_000612"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ7799079.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLTGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPGIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGDSLRMIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            complement(1468..1704)
                     /gene="acpP"
                     /locus_tag="WJL87_000613"
     CDS             complement(1468..1704)
                     /gene="acpP"
                     /locus_tag="WJL87_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ7799080.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            1936..2943
                     /locus_tag="WJL87_000614"
     CDS             1936..2943
                     /locus_tag="WJL87_000614"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ7799081.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALYTVYKLGLIPVTGNRTKPV
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPKPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDTMIRNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVYGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGRPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGRDAADKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            3276..3599
                     /locus_tag="WJL87_000615"
     CDS             3276..3599
                     /locus_tag="WJL87_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799082.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            3686..3967
                     /locus_tag="WJL87_000616"
     CDS             3686..3967
                     /locus_tag="WJL87_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799083.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            4020..4493
                     /locus_tag="WJL87_000617"
     CDS             4020..4493
                     /locus_tag="WJL87_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ7799084.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            4747..5082
                     /locus_tag="WJL87_000618"
     CDS             4747..5082
                     /locus_tag="WJL87_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799085.1"
                     /translation="MTVDKEQVEKMIYSENKQSVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLDIARLSLTCFGHLARMHENIGDCDKVIALLLSKQGDPDFQGFAEDALDEI
                     SLFIFKKRP"
     gene            5262..7952
                     /gene="glnE"
                     /locus_tag="WJL87_000619"
     CDS             5262..7952
                     /gene="glnE"
                     /locus_tag="WJL87_000619"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ7799086.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKALVRPFVFRKYLDYGAYEAMRKLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAETGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDQQTQTLPTSPEQRQLLAESMGFDSYSAFSDGL
                     NVHRNKVNQLFNEILSEPEEQTQDNSEWQWAWQDKPDEEGRRCRLKAHGFDAETVAAR
                     LDQIRHGHKYRHLSAHAQPRFDAVVPLFVQAAAAQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DDLKACGGDTEAQMDTLRRFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     LCAWADMPKKHRDTPQFAVVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTSEIQTAFDRIRTEILTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNISADCGLIDKTLAGQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVTGELLAHYGNVRKLWREVFGEEAATA"
     gene            complement(8252..8944)
                     /locus_tag="WJL87_000620"
     CDS             complement(8252..8944)
                     /locus_tag="WJL87_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ7799087.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(9052..10245)
                     /locus_tag="WJL87_000621"
     CDS             complement(9052..10245)
                     /locus_tag="WJL87_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ7799088.1"
                     /translation="MSDQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(10374..11111)
                     /gene="pxpA"
                     /locus_tag="WJL87_000622"
     CDS             complement(10374..11111)
                     /gene="pxpA"
                     /locus_tag="WJL87_000622"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEZ7799089.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGQVKAVDGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(11098..12027)
                     /locus_tag="WJL87_000623"
     CDS             complement(11098..12027)
                     /locus_tag="WJL87_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ7799090.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDYLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQAYLNQIRRITHEAG"
     gene            complement(12024..12671)
                     /gene="pxpB"
                     /locus_tag="WJL87_000624"
     CDS             complement(12024..12671)
                     /gene="pxpB"
                     /locus_tag="WJL87_000624"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ7799091.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            12855..15062
                     /gene="uvrD"
                     /locus_tag="WJL87_000625"
     CDS             12855..15062
                     /gene="uvrD"
                     /locus_tag="WJL87_000625"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ7799092.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPVDVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FILDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKIAAFVRLIEALRNQVGQLPLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFEILPENISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSIKKTAYDSYGNTRQTATFKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEEM"
     gene            15297..15755
                     /locus_tag="WJL87_000626"
     CDS             15297..15755
                     /locus_tag="WJL87_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ7799093.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAKDHVQICEVSGIGFEKEGSLCGAMGQK
                     LWLEEWEYPPIGVYFANDPSGGHAMFALDYRECGRDGEPKVVFVEQESDF"
     gene            complement(16026..16241)
                     /locus_tag="WJL87_000627"
     CDS             complement(16026..16241)
                     /locus_tag="WJL87_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799094.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNPQSVQELEQHLIEAYDLMDTTG"
     gene            complement(16364..16722)
                     /locus_tag="WJL87_000628"
                     /pseudo
     CDS             complement(16364..16722)
                     /locus_tag="WJL87_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
     gene            16775..>17004
                     /locus_tag="WJL87_000629"
     CDS             16775..>17004
                     /locus_tag="WJL87_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7799095.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTATEPDSIVYTDCYRSYDV
                     LDVSEFSHFRINHSTHFAERQNHINGIENFWNR"
BASE COUNT         4241 a         4533 c         4345 g         3885 t
ORIGIN      
        1 ctataaaaat gccgtctgaa acggtttcag acggcatttc gatgtcggcg gcggctttgc
       61 ggaatcagcc tttgaagcgt ttgaagacca gcgtgccgtt ggtgccgccg aagccgaagg
      121 agttggaaat ggcaacgtcg atttccgcgt cgcgcgcttc gttggcgcag tagtccaaat
      181 cgcagccggc ttcaacgtct tgttcaaaaa tgttgatggt cggcgggatt ttgccgtcgt
      241 gtatcgccaa aatgctgtac acggcctcca cgccgcccgc cgcgccgagc aggtggccgg
      301 tcatggattt ggtcgagctg acgacggttt tgtaggcgtg ttcgccgaac gcgcgtttga
      361 gggctttggt ttcgttggca tcgcccaagg gggtggacgt gccgtgcgcg ttgacgtaat
      421 ccacgtcttc gggattgatg ccggcatctt tcagcgcgcg ggtaacggca agggcggggc
      481 cttcttcgtt cggcgcggtg atatggtaag catcggaact catgccgaag ccgacgattt
      541 cggcgtagat tttcgcgccg cgttttttgg cgtgttccaa ttcttccaac accaatatgc
      601 ccgcgccttc gccgataacg aagccgtcgc ggcctttgtc ccacggacgg gaagcggtgg
      661 cggggtcgtc gttgcgggtg gagagggctt tcatcgcggc aaaaccgccc acgcccaaag
      721 tgctgattgc gccttccgcg ccgccggcaa ccattatgtc cgcgtcgccg tatttaatca
      781 tacggaggga atcgccgatg gcgtgcgcgc cggtggtgca ggcggaaacc atcccgtagc
      841 tcgggccgcg gtagcctttg aggatggtaa cgtgtccgga aatcagatta atcagagaac
      901 cggggataaa gaaagggttg attttgcgcg cgccgccttc gattacggct ttgccggtga
      961 cctcgatgcc gggcagtccg ccgatgccgg aaccgatgtt cacgccgatg cggtctttgt
     1021 cgaggttttc cacatcgtcc aaacccgaat cggcgattgc ctgcaatgcg gcggcaatgc
     1081 cgtagtggat gaatacgtcc atccggcgcg cttctttcgc gctgatgtat tgtccgatgt
     1141 cgaaaccgcg cacctcgccg gcgacacggc tgttgatgtc ggatgcgtca aagcgggtaa
     1201 tcgcgccgat gccgcttttg ccggtgagca gggtgtccca agcctctgcg acagtgttgc
     1261 cgacagggga aacctgacct aagcctgtaa tgactactct tctctgactc atgataacct
     1321 cgctgttggt tgtcggaatg ggggcatatg cggctgtcgt gcagatgccg tctgtaattt
     1381 gcggcagggg ttcaaacagt ttgccatata agggaaaagc ctctattgcg cggtgcagca
     1441 gaggctgttg tgtcgggcga cgaccggtta gccgttgtgg gcattgatgt agtcgatagc
     1501 cagttggacg gtggtgattt tttcggcatc ttcgtcgggg atttcgcagc cgaatgcttc
     1561 ttccaaagcc ataaccagct ccacggtgtc caaagaatcc gcgcccaagt cgtcttggaa
     1621 agaagattcg tttttcacgt cggcttcgtt tacgcccagt tgttcagcaa caattttttt
     1681 aacttgttgt tcgatgtttg acatatcagt cgttccttta tgccttgcgg caggttgttt
     1741 aagggaaata aatcggtggt attgtaccga cttttaatag agttttctat ctaatgacta
     1801 ttatatcaat atttgccgat ttgtacattt ttgggtgcgg cgggttttgt cgttcaagtt
     1861 tgacctgtgt gccgtatgtt tgacgggatt tcggttaaaa tgacggcatt tccatctgaa
     1921 gcagaaagcc ctgtcatgta tccacttgcc cgccgcatcc tgtttgcact cgatgccgag
     1981 aaagcccacc acttcacgct cgacgcgctc tacacggttt ataaattggg tctgattccc
     2041 gtaaccggca accgtaccaa acctgtaaaa ttgatgggta tggatttgcc caaccctgtc
     2101 ggacttgccg ctggactcga caaaaacggc gaatacatcg acgcattggg cgcgctcggc
     2161 tttggtttca tcgaaatcgg cacggtaacg cccaaaccgc agcccggcaa cccgcagccg
     2221 cgcctcttcc gcgttcccga acaccaaggc atcatcaacc gcatgggttt caacaaccac
     2281 ggtatcgaca ccatgatacg caacatcgaa aaaagtaaat tcagtggcgt attgggcatc
     2341 aacatcggta aaaacgcggt tacacccatc gaaaacgctg ccgatgatta tttaatctgc
     2401 cttgaaaaag cctacgcaca cgcaagttac attaccgtca acatttcctc gcccaacacc
     2461 aaaaacctcc gcgcgctgca aggtggcgac gagttgagcg cattgcttga ggctttgaaa
     2521 aacaaacagg cacagcttgc ctctgtatac gggaaatacg ttccgctcgc cgtcaaaatc
     2581 gcccccgatt tggatgaagc acaaatcgaa gacatcgccc acgttgtcaa atccgtcgaa
     2641 atggacggca tcatcgctac caataccacc atcgacaaat caagtctcgg cagccatccg
     2701 ctcgcaggcg agcagggcgg tttgagcggg cggcccgttc atgaaaaaag taatcgggtg
     2761 ttgaagctgt tggcagacca catagacggc aagctgccga ttatcggcgt aggcggcatt
     2821 atggaaggca gggacgcggc agataaaatc cgcttgggcg caactgccgt ccaggtgtac
     2881 agcggattga tatacaaagg tccggcattg gtcaaagaat gtttgaaggc tttggcgcga
     2941 tgacgcgatc cgcccaaaat gccgtctgaa cgcacgtttt gccgttcaga cggcattttc
     3001 atttcctttt tccgcctgcc gccccttgaa aatcccttac gcgccgccct gtttgaaaca
     3061 aagcaaacgc ccgccgcgaa ccggacaatc ttgatacgca gccaaagcaa gtccggcggt
     3121 aagttgttaa atatataagc aattgataca aaaaataatt ttatgccgtg gtggacggtt
     3181 tgagaaaacg caaaatatga acggcgttga aattcatgat gtggaaaata aggatacggc
     3241 ggataatcaa aagattttat atttaaggat ttgatatgtt taaggacgaa ctaaatgaat
     3301 ttatccgatt gatatccgat ccggaaagtg aattggatga atggtatttg agtgatttta
     3361 aagatgaaca tatatgggaa atgcaaagtt acgaagcatt ttcatgtttg cgggaagcag
     3421 tcccctattt gtttgcttat ccccgatacg ggtatgaact gttggagata atttccgcgt
     3481 taaaagaaac atctgatacg acagaattat tttatgagcc gggtatcgtg cctttgctga
     3541 ttgatttata taaagaagat agttatctgg taaatatggt caaacggata ttcaaatgat
     3601 gaaaggggcg cgggcgtttg cttggcggaa ggcaggtaac tcaatttgaa ggacgcggaa
     3661 gccgatacgt taaaaaggat gaaaaatgga aaacgggcaa cggaataacc ggtttccttt
     3721 ggaaaaacga attttttatc tggagcattc ggggcggtat ctgatgattt gcgccttatc
     3781 ggattattcc caaaacaaac atactgtcgt tatggcaaat ttcatctatc cggatgaaaa
     3841 aacggattgg cggaatttgg atgatttatt caatgagttg gttttagagg aattgcaggc
     3901 ttcatttatg gattggcatc cgactgttga agaggcaatc agccgtcatt tggaagactt
     3961 ttcgtaacag gcgtcaatcg gaaggggcgg aatgacgggg caggcaatcg gagtaaaaaa
     4021 tgaaccttga tttaaccgcg caaaaagtcc gtctttcttg gaaggatatt ctgtgggggt
     4081 atgggaataa atacttgggt tgggctgatg tggcagctta tgcccgaaaa atgacgcttt
     4141 cagatcatga tgaacgtgtg ttcaaactat ctttaatcaa caaatccaat attcttgaat
     4201 taaagcctgt tctggaagat ttggcttcgg aaatgaggga ttattcccct aaaaattggc
     4261 tgtacgtcct cttaagcgat gtattccata gaaaagaaga atttgaggat cctttggggg
     4321 aagttgaaaa aatttatgca gattttgatt atccggaaga aatagaatca tttgtcaggt
     4381 atatgccgcc caaagacggt tatattcctt ctgcccacac ctatgaagaa aatattgccc
     4441 ggttatattc tcactgggaa cactatttga acaacggcgg agggcagggt taaaaccggc
     4501 aatccgatgc cgtctgaagc attatccggc cttcagacgg caaacggatt agagcggaag
     4561 atttgaaagc gggggcgggc tgcattccgg aagcggcaaa acccgaaccg cccgcatcgt
     4621 tgccgataaa ccgataagga aatcgttttg ccggaccggg tgcaaatgtt tgataacaat
     4681 cctgccgccc ggcaaaaatg ctttaaagaa agcaggattg gttaatttaa aaggaaaaat
     4741 taaaaaatga cggttgataa ggaacaagta gaaaagatga tttattctga aaataaacag
     4801 agtgttattg acggtatgct cggtatgacc ttcagctcgg aggaggatga aatcccctgg
     4861 atttccgaga agttgacgga gttgtcgaaa cataaagact tggatattgc cagattgtca
     4921 ttaacctgtt tcggacatct cgccaggatg catgaaaata tcggcgattg cgataaagtg
     4981 attgctttat tgctctcaaa acaaggtgat ccggattttc aaggttttgc cgaagatgcg
     5041 ttagacgaga tttctttatt tatatttaaa aagcgtccgt aagcgcggat taaacctgtc
     5101 aatccgatgc cgtctgaagc gttatccggc tttcagacgg catcgaaggt tgctatgata
     5161 tagtggcttg actttaaacc ggtacggcat cccctcgccc tgtcctgatt tgaagtgaat
     5221 ccgccatcat cttcctgcca ttttttcaaa cggaatccga aatgtccgac aaccgcctcg
     5281 acaccgcccg ccgccattcc ctcttcctcg cccgccagct cgacaacggc aaactcaagc
     5341 ccgaaatttt cctgcctatg ctggacaagg ttttgaccga agcggatttc caagcctttg
     5401 ccgactggga caaaatccgt gcggaagaaa acgaggaaga attggcgcgg cagttgcgcg
     5461 ggttgcgccg ttatgtggtg tcgcagatta tcgtgcgcga tataaaccgc atcagcgatt
     5521 tgaacgaagt aacccgcacg attacgctgt ttgccgattt tgccgtcaat accgcgctgg
     5581 attttgccta cgcctattat cgggacatgt acggcacgcc gatcgggcgt tataccaaat
     5641 cgccgcagca tttgagcgtg gtggcgatgg gcaaggcggg cggctatgag ttgaacgtgt
     5701 cttccgacat cgatttgatt ttcgtctatc ccgaatcggg cgacaccgac ggcaggcgcg
     5761 aacgcggcaa tcaggagttt ttcaccaaag tcgggcagaa actgattgcg ttgctgaacg
     5821 acattaccgc cgacgggcaa gtgttccgcg tcgatatgcg gctgcggccg gacggtgatt
     5881 cgggcgcgtt ggtattgagc gaaaccgcgc tggagcaata tttgattaca caggggcgag
     5941 aatgggaacg ctacgcgtgg tgcaaaggtc gcgtggttac gccgtatccg aacgacatca
     6001 aagcactggt gcgccccttt gtgttccgca aatatctgga ttacggcgcg tatgaggcga
     6061 tgcgtaagct gcaccgccaa atccgcagcg aagtcagcaa aaaaggcatg gcggacaaca
     6121 tcaaactcgg cgcgggcggc atccgcgaag tcgaatttat cgcccagatt ttccagatga
     6181 tacgcggcgg ccaaatgcgt gcgctgcaac tgaaaggcac gcaggaaacg ctgaagaaac
     6241 ttgccgaaac cgggatcatg ccgtctgaaa acgtcgaaac cctgcttgcc gcctaccgct
     6301 tcctgcgcga cgtcgaacac cgcctgcaat actgggacga ccaacaaacc caaaccctgc
     6361 ccacttcgcc tgaacagcgg caactgctcg ctgaaagcat gggtttcgac agttattccg
     6421 ctttttcaga cggtctcaat gttcatcgga acaaagtcaa tcagttgttc aacgaaattt
     6481 tgagcgaacc cgaagagcaa acgcaagaca acagcgaatg gcaatgggca tggcaggaca
     6541 aacccgacga agaagggcgg cgatgccgtc tgaaggcgca cgggttcgat gccgaaaccg
     6601 tcgccgcaag gctcgaccaa atccgccacg gccataaata ccgccatctt tccgcacacg
     6661 cccagccgcg tttcgatgcg gttgtgccgc tgttcgtaca ggcggcggca gcgcaaagca
     6721 acccgaccga tacattgatg cggctgttgg attttctcga aaacatcagc cgccgatccg
     6781 cctatctcgc cttcctcaac gaacatccgc aaaccttggc gcaactggcg cagattatgg
     6841 gccaaagttc ttgggtggcg gcgtatctga acaaatatcc gattttgttg gacgaactca
     6901 tcagcgcgca gcttttggat accgcgtttg attggcaggc gctcgccgcc gccctttcag
     6961 acgacctcaa agcctgcggc ggcgatactg aagcgcaaat ggacaccctg cgccgcttcc
     7021 agcacgccca agtcttccgt ctcgccgtcc aagacctcgc cggactgtgg acggtagaat
     7081 ccctctccga ccaactctcc gccctcgccg acaccatcct cgccgccgcc ctgctgtgcg
     7141 catgggcgga catgcccaaa aaacaccgcg acacaccgca attcgccgtc gtcggctacg
     7201 gcaaactcgg cggtaaagaa ctcggctacg cctccgacct cgacctcgtc tacctctacg
     7261 acgatcccca ccccgacgca ggcgacgtgt acagccgcct cgcccgtcgc ctgaccaact
     7321 ggctttccgc cgccactggc gcaggcagcc tctacgaaac cgacctgcgc ctgcgcccta
     7381 atggcgacgc cggtttcctc gcccacagca tcgccgcctt tgaaaaatac cagcgcgaaa
     7441 acgcctggac gtgggaacac caatccctta cccgcgcccg cttcatctgc ggcacgtccg
     7501 aaattcagac ggccttcgac cgcatccgca ccgaaatcct caccgccgaa cgcgaccaaa
     7561 ccgccttggc aggcgaaatc atcgaaatgc gcgaaaaaat gttccccacc cacccgcctg
     7621 ccgacagcaa cgtcaaatac gcgcgcggtg gcgtggtcga tgtcgaattt atcgtccaat
     7681 atctgatact tgcccatgcc cgccagtatc cgcaactctt ggacaactac ggcaacatcg
     7741 ccctcttaaa catctccgcc gactgcggtt tgattgacaa aaccctcgcc ggacaaagcc
     7801 gcaccgccta tcgcttctac cgccggcagc agcacaacac caaactgcgc gacgcggcaa
     7861 aaaccgaagt aaccggcgaa ctgttggcac attacggcaa tgtcaggaaa ttgtggcggg
     7921 aagtgttcgg cgaagaagcg gcaaccgcct gaacaaaaaa tgccgtctga agcctgacaa
     7981 tctgggtttc agacggtatt ttcgtaccgt gccgttttaa ggttgcggca gagctaaagc
     8041 gatttatcgg gaatggctga aacccaaaaa ccggattcct ctttcagtta cagcagcttg
     8101 atccctttcc cttatccaac gggggaaggc taggataggg tggcttgcaa atatacagaa
     8161 caagggacaa gagccaccct ctctccaacc ctctccctcc gtacgggagg gggtggattc
     8221 tcgcgggcga agcccacgct acggttagcc tttaccccag cacaaacaat tcccgcccgt
     8281 gcgccttcag ccaactttta gcattgtcgg tatgcggcgt cagcgtgttc accaaatgcc
     8341 aaaagcgcgg actgtggtcg gggtggcgga ggtggcagag ttcgtggatg cagacatagt
     8401 cggcgacgta ttcgggcgtg ccgatcagcc gccagttgag gcggatgccg gtgtgcgggc
     8461 ggcatacgcc ccaaaaggtt ttggcgttgc tcaggtctgt ggcggtgggc gtcagtcctg
     8521 tttcggctgc gtgtttttca aggcggggca gcaggtattc gcgggcgcgt tcgttcaaca
     8581 ggcggcgcag gtggtcgatt tgtgcggcgg tttcttttcg gggaagcagg atttcagacg
     8641 acgtgatacg gatatggctt tggctgtggg tatccagctt ggtctttatt ccccgatacc
     8701 aaatccactc gggtaagttt gggtgggaaa caggatgcac gggcgttttg gcaagcgtgt
     8761 tccgcaaaat cgtttcgttt gccgccagcc agtttgctaa cgcgtggtct tgaaaaaagg
     8821 gtgggacgtt gatgctgacc gtctgcatat tgacggggcg cagaatcaga tttttcttgg
     8881 cactgcgttt gagttcgatt tcgatgcaca aaccgtcgga aagagtatag gtgaagcgtt
     8941 tcatagttgt gaataggttt cagaccggat acatcgtctg aaacaggaat tttccatatc
     9001 aggcggcaaa cttcggataa tatacaaaat caaacatctg cgctacaagg ttcagccgaa
     9061 caagccgccg atatatttgc tgatggtgat ggcgctgagt actgccatca aaccgaccac
     9121 aatcacgccg gaaacggtga gccacagcgg gtgtttgtag tcgccgacaa ttttggtttt
     9181 gtaggcggca atcagaatca gaccgaggga aatcggtaaa atcaggccgt ttaatgcgcc
     9241 tacgaacacc agcacctgcg ccggtttgcc gatggtggaa aatacggcgg tggacacggc
     9301 gataaaggca ataatccatt tgtttttatt gcgttcgata gacgggctga gaccggagaa
     9361 gaacgacacc gaagtataag ccgcaccaat caccgaagta atcgaagccg cccaaatcac
     9421 cacgccgaaa atcagcaggc cgatgtatcc cgccgcatat tcaaacggtg tggaagcagg
     9481 gttgtcggga ttgagctgta cgccttggct gaccacgccc aaaaccgcca aaaacaatac
     9541 aatccgcata atcgaggcaa tcaggatcgc ccgcaccgag ctttggctca cttccggcaa
     9601 cgccgatttg cctttgatac ctgcgtccag cagacggtgc gcaccggcga aggtgatgta
     9661 gccgccgacc gtgccgccca ccagtgtaac aatcgccatt gcatcgagtt tttccggcat
     9721 aaaggtatgc acggcggcat ctgccagcgg cggattcgcc tgccatgcca cataaaccgt
     9781 cagcgcaatc attacgaaac ccatcacttg ggcgaatttg tccatcactt tgcctgcttc
     9841 tttaaacaga aacacaccga tggcaatcac gccgctgatc acggcaccgg tttccggtga
     9901 cagtccggtc agcaggttca gacccaagcc tgcgccgccg acgttgccaa tattgaacgc
     9961 caaaccgccc atcacaatca gcacagccaa gaaatagcct gcgccgggca agacctgatt
    10021 ggcaatatcc tgcgcctgtt tttcggaaac ggcgacaatc cgccaaatat tgagctgcgc
    10081 cccgatgtcg agcagaatcg agagcagaat cacaaagccg aaacttgccg ccagtgcttg
    10141 ggtgaaggtg gcggtttggg tcagaaagcc cgggccgatg gcggaagtcg ccatcaggaa
    10201 tgcagcgccg attaaggcat ttctgcggtt tttttgatca gacataatcg cttatcctct
    10261 ataaaattgg ttgttgctgt gtttgggcga aacctgcggt tttagctacg cagaaactcg
    10321 ctttgctcgt tttggcgaaa cctgcggttt tcagacggcc tatgaactgt ttttcaagca
    10381 gaaactttga tgcctgccgc cagtagttcc tgccggattt tttcggcaaa caccacggcg
    10441 tgcggcccgt ctccgtgcag acagatgctg tcggcttgca cggcaaccag gctgccgtcc
    10501 actgctttga cctgcccgtc ccgcaccatc tgcaatactt gggcgatggc ttcttcgtcg
    10561 ctgtccacct gcgcatcggg gcggctgcgg ggaaccagcg taccgtcggg catatagcgg
    10621 cggtcggcga atacttcgga aatcacaccc aagcctgcgg cttttccggc ttccaagagc
    10681 aggctgccgg aaagtgccat caatttcaat ttcgggtcga aatccgccac aattcgggca
    10741 acggtatccg ccagcgcacg gtttttcgcc gcttgattgt acattgcgcc gtgcggtttg
    10801 acataagcca tttccaaacc ctgatcacgg cacaaggcct gcaatgcgcc caactggtaa
    10861 ttcagacacg cccgcaaatc ggcttcggac agattcattt cggtacggcc gaagttttcc
    10921 cgatcgggat agccggggtg tgctccgatg cgcacgccgt tttgttgggc atacgccaat
    10981 gccgcccgaa tatcggcaat gctgccggcg tgttgggcgc aggcgatgtt ggccgaagta
    11041 atcagctgca acaaggcttc gtcgctgccg cagccttcgg cgagatcggc gtttaaatca
    11101 acctgcttca tgggtgattc tccgtatttg gttcagatag gcttggtttt tgcgccgcag
    11161 ggcggtggct tctttcaagc cgattatttt gaatttgact ttgctgccga agcgcacctg
    11221 tgccagcctg cccaaatcgg cggcggcaac ggtagcgatt ttcggataac cgccggtggt
    11281 ttgcgcatcg gccagcagga taatcggttt gccgccgggc ggcacctgca cggttcctgc
    11341 ctgaacagcg tgggacagca tttccaaagg ttgcgacagg gtcagcggct gtccgtcgaa
    11401 gcggtagccc atgcggttgc tatcgctttg cagcgtccac gtttcccgtt ccagattcag
    11461 acgccctttt tcactgaaag cggcatattc cgacgaagga acaaggtgga cggtatcggt
    11521 aaacggtatc ggggcaatgc cgactttgga caattcctgc gcacctttgc cgatggggag
    11581 ataatcgcct ttttgcagca ttctgccctg atggccgccg aaaccggctt tcaggtcggt
    11641 acttctcgaa cccatcactt ccggcacatc aaatccgccc gccacgcaca catagccgta
    11701 catgccctgc acggcacgca ccattttcaa ggtctgccct ttgcgggcgg tataacgcca
    11761 atacgaatag accggttcgc cgtccaattc cgcctgatac acggcaccgg tgagacaaaa
    11821 cggcgtatcc cgttcaaaca ccagcattat cccgcccaaa gcgatttcga ttgcggccgt
    11881 gccttcgtcg ttgcccaata aaatattacc cgccgccaaa gcaaccgtgt ccatcgcacc
    11941 ggcatgaccg atgccgtaac gccggtgtcc gtagcgtccg gtatcctgaa tatgcgccgg
    12001 tgcctgcact gccgaaacgt gaatcatggc tcaatccttt ctgcaacaaa gcggacttgg
    12061 tcacccgccg ccagcagggt cggcggattc aaatcggctc ggaacaaggg taattcggtt
    12121 ctgccgataa tctgccagcc gccgggcgaa gcgaacggat acacaccggt ctgactgccg
    12181 ccgataccga ccgaaccggc aggaacggac gttctcggca cggcacggcg gggcgtgtgc
    12241 aatgcttcgg gcaagccgcc cagataaggg aaaccaggct ggaagcccat cataaatacg
    12301 gtataagttt gcgccgtatg gcggcggacg atttcggaaa taaccgtctg atggaaagca
    12361 gcgacttccg ccaaatccgg gccgtattcg ccgccgtagc agacgggaat ttccaccagt
    12421 ttgccctgat ggtctgtaac ggcggtgtgt tcccacacat attgcaattc atcggcaagc
    12481 gtcgccaaat cggtatcgaa acgggtgaac acggtcagat tgttcatgcc gaccaccact
    12541 tcctcaatcc tgtcgtgctg cccgagcgca gcggcaaacg cccacaactt ttgctgtttg
    12601 cccagttcgg aaggtgcatt cagtcggcag accaaagcgg attcgctgat tggtgtgatc
    12661 tctattctca tttgttgttc attttggtta tgttttaatg aatctatatg caggggcggc
    12721 ggtttgtcaa tatcttctgt gctgcatcat caaaccgtcg attggaaaag tgctgccctg
    12781 ccgctgcact ttttcagacg accttaaacc gtttctatta aaatagcgca ttccactttt
    12841 cagacggcat ctctatgttt cccgaccaat ccgcccccaa cctgctgcaa ggcttgaacc
    12901 ctgaacaact ttccgccgta acctggccgc cgcaatccgc ccttgtgctg gcaggcgcgg
    12961 gcagcggcaa aacgcgcgtg ctgaccacgc gtatcgcatg gcttttgcaa accggtcagg
    13021 caagcgtgca cagcattatg gcggtaacgt ttaccaacaa agccgccaaa gaaatgcaaa
    13081 cccgactcgg cgcgatgatt cccgtcgacg tccgcgctat gtggctcggc acgttccacg
    13141 gcctctgcca ccgctttcta cggctgcacc accgcgatgc cggtttgccg tcttcctttc
    13201 aaatcctcga cggcggcgac cagctttccc tcatcaaacg cctgctcaaa agcctcaaca
    13261 tcgccgaaga aatcatcgcg ccgcgttcgc tgcaaggctt tatcaacgcg caaaaagaat
    13321 ccggtttgcg cgcttccgtc ttgagtgcgc ccgatccgca cacaagccgc atgattgaat
    13381 gctacgccga atacgacaaa atctgccaac gcgaaggcgt ggtcgatttt gccgaactca
    13441 tgctccgcag ctacgaaatg ctgcaaagca acgaaatcct gcgccagcat taccaaaacc
    13501 gcttcaacca cattctcgtc gacgagttcc aagacaccaa caaactgcaa tacgcctggc
    13561 tcaaactcat ggcgggcgga aacgcagcgg tatttgccgt cggcgacgac gaccaaagca
    13621 tttaccgttt ccgtggcgca aacgtcggca acatgaccgc gctgatggaa gaattccaca
    13681 tcgacgcgcc cgtcaaactc gaacaaaact accgctccgt cggcaacatc cttgccgccg
    13741 ccaatgccgt gattgaaaac aacgacgaac gactcggcaa aaacctgcgc accgacgccg
    13801 aagcaggcga caaaatccgc tactactccg cctttaccga cctcgaagaa gcccggttca
    13861 tcttggacga aaccaaagcc ctcgaacgcg aaggctggga tttggacgaa atcgccgtcc
    13921 tctaccgtag caacgcccaa tcccgcgtta tcgaacaaag cctgttccgc agcggcatcc
    13981 cctacaaaat ctacggcggc ttgcgctttt acgaacgcca agaaatcaaa cacgcgctcg
    14041 cctacctacg cctcgccgtc aatcccgacg acgacaacgc ccttttgcgc gtcatcaact
    14101 tcccaccgcg cggcatcggc gcacgcaccg tcgagaacct tcagacggcc tcaaacgaac
    14161 aaggcatcac cctttggcag gcagcctgca acgccggcgc gaaagccgcc aaaatcgccg
    14221 ccttcgtccg cctgattgaa gcactgcgca accaagtcgg acaactgccc ctgtccgaaa
    14281 tcatcgtcgg catcctcaaa gacagcggcc tgaccgaaca ctaccgcacc caaaaaggcg
    14341 acaaccaaga ccgtctcgac aaccttgacg aactcgtcaa tgccgccatc gagttcaaac
    14401 ccgaagacag caacttcgaa atcctgcctg aaaacatttc agacgaccca gccttcccca
    14461 tcctcgcctt cctaagcaat gccgccctcg aatccggcga aaaccaagca ggcgcgggcg
    14521 aaaaagccgt ccaactcatg accgtccacg ccgccaaagg cttggaattt aacgccgtct
    14581 tcctcaccgg catggaagaa ggccgtttcc ccagcgaaat gagccttgcc gagcgcggcg
    14641 gcctcgaaga agaacgccgc ctcatgtacg tcgccatcac ccgcgcccgc aaacgcctct
    14701 acatcaccat ggcgcaacaa agaatgctgc acgggcaaac ccaattcggc atcgtctccc
    14761 gcttcgtcga agaaatcccg cccgaagtat tgcactacct gtccatcaaa aaaaccgcct
    14821 acgacagcta tggcaacacg cgtcaaactg ccacgttcaa agacaaaatc atcgacgact
    14881 acaaacagcc gcaaacctac gcaggcttcc gcatcggaca aaacgtccgc cacgccaaat
    14941 ttggcaccgg cgtgattatc gatgccgcag ataaaggcga atccgcccga ctgaccatca
    15001 atttcggcaa acagggcgtg aaagagttgg acaccaagtt tgcgaaattg gaagagatgt
    15061 agaagggaga tggcagaggc cgtctgaaaa cgggaacaac aatttttgcc caaacctccg
    15121 ccgtcattcc cgcgcaggcg ggaatccaga cctttcggca cgggaactta tcggataaaa
    15181 ggtttcttta gattccacgt tctagattcc cgcctgcgcg ggaatgacgg acggtaaaag
    15241 ccgggtatag atacctaacc cttgtttata gctgaaaatc aataaggaag aacattatgt
    15301 cccaagtttt taaagatttt gatttgtcct ccgtatggga atccgacagt tgggcagatg
    15361 aaaactacaa agaagccccg tttacccctg aaattttggc tgccgtagaa agtgaactgg
    15421 gctataaatt gccgcaaagt tttattgaat tgatggcggt acaaaacggc ggaatatttg
    15481 tcaaaaactg ttttccgacc acacagagaa attcgtgggc gaaagatcat gtgcaaattt
    15541 gcgaggtatc gggaatcggt tttgaaaaag aagggagttt gtgcggcgcg atggggcaaa
    15601 aactttggct ggaagaatgg gaatacccgc ctatcggcgt gtattttgcc aacgacccgt
    15661 caggcggtca tgccatgttt gccttggact atcgggagtg tggaagagac ggagagccga
    15721 aagtggtgtt tgtcgaacaa gaatcggatt tttaaatcgt cgaacttgcc cccgattttg
    15781 aaacctttat tcacagcttg cggcatgaag atgagtttat tgacgaggaa atataaaacg
    15841 gcggttgaaa aaccagaatc atcaaaggaa aacgggcgga ataacagata accgattgaa
    15901 tccgtaagga aaacggtttg taggaacgtg ccatccaaga cctttgcaaa gaaaaactgt
    15961 ccccgacagc attgacatta ttaacagaac ttatcaattt tggagctatc tcaaatataa
    16021 ttcggttatc ctgttgtatc cattaaatca tatgcttcaa ttaaatgttg ttctagctct
    16081 tgcactgatt gaggattgcg aattcctgat acaatctcaa attcttctgc atctatgcaa
    16141 acacctgcat aaatttcaat aacaagggaa cgcaataatt gaagctcttc tcttgttaaa
    16201 gaaataataa tgtcatcacc tgtgtaattg attatattca taataatttt atttttgctt
    16261 gtcaaagtaa gttttgccta aggttggtct caatgcttcc caccatcttt tgaatttggg
    16321 tctctgatta caaccgttcc agacttatca tcccaaattg ctcttatgtg tttggattgt
    16381 aatcttcgaa ttcccaagaa aaaaatcgta ataagtttga aagtgtcaaa tcccaagttt
    16441 cttttgagca atattctaat attttatcaa tttcactttt aataatctta tgatcaaact
    16501 gttctaatat taatgcatta gaccaaaaaa accttcttta ttacaatgat gggaaatcca
    16561 tttaggagaa caaatgcaaa gtgaaaaaat agatgagcct tgttctcctt cgattccgat
    16621 atccaaatct atccatctat ggaaattatc tggaatttcg ggggtaaatt tttcaaaatc
    16681 aatatcatat aaatttatgc tttttaaatc caatttaatc attagggctg tcctagataa
    16741 atagggaaat tcaaattaag ttagaattat ccctatgaga aaaagtcgtc taagccggta
    16801 taaacaaaat aaactcattg agctatttgt cgcaggtgta actgcaagaa cagcaacaga
    16861 gcctgacagc attgtttata cggattgtta tcgtagctat gatgtattag atgtgagcga
    16921 atttagccat ttccgtatca atcacagcac acattttgct gaacgacaaa accatatcaa
    16981 tggaattgag aatttttgga accg
//
LOCUS       DAWKST010000027        16912 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.027, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000027 DAWKST010000000
VERSION     DAWKST010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16912
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.027"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(65..904)
                     /locus_tag="WJL87_000630"
     CDS             complement(65..904)
                     /locus_tag="WJL87_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ7799096.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1189..1264
                     /locus_tag="WJL87_000631"
     tRNA            1189..1264
                     /locus_tag="WJL87_000631"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Arg,seq:ccg)
     gene            complement(1329..2522)
                     /locus_tag="WJL87_000632"
     CDS             complement(1329..2522)
                     /locus_tag="WJL87_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ7799097.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAATPVPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIATVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFESAGFEVGTYPYYDPAAVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2528..3631)
                     /gene="trmA"
                     /locus_tag="WJL87_000633"
     CDS             complement(2528..3631)
                     /gene="trmA"
                     /locus_tag="WJL87_000633"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ7799098.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKAGGASLIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKRLDNEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRTNLDTLAETH
                     TVERAALFDQFPFTHHIESGVLLKKKILGKSKR"
     gene            3774..4874
                     /gene="aroC"
                     /locus_tag="WJL87_000634"
     CDS             3774..4874
                     /gene="aroC"
                     /locus_tag="WJL87_000634"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ7799099.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRRETDQVEILSGVFKGKTTGTPIALLIRNTDQRSKDYGNIAQSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAVDTPDIARSDK"
     gene            4965..5390
                     /locus_tag="WJL87_000635"
     CDS             4965..5390
                     /locus_tag="WJL87_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ7799100.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDAALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETPSVEAEAAESSAAE"
     gene            complement(5464..7449)
                     /gene="parE"
                     /locus_tag="WJL87_000636"
     CDS             complement(5464..7449)
                     /gene="parE"
                     /locus_tag="WJL87_000636"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ7799101.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPIFSCENYISDGHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVSNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVATVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7516..8040)
                     /locus_tag="WJL87_000637"
     CDS             complement(7516..8040)
                     /locus_tag="WJL87_000637"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ7799102.1"
                     /translation="MLDREGYRPNVGIILTNNRDEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLSQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQLSGNR"
     gene            8205..9500
                     /gene="serS"
                     /locus_tag="WJL87_000638"
     CDS             8205..9500
                     /gene="serS"
                     /locus_tag="WJL87_000638"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ7799103.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLEAVQKELDAWL
                     LSIPNLPHEDVPFGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVADSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYEALEEMVGHAENILKALELPYRVITLCTGDMG
                     FGATKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9578..10576)
                     /locus_tag="WJL87_000639"
     CDS             complement(9578..10576)
                     /locus_tag="WJL87_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ7799104.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAHDPFCNPEAEKIGGRYVDLDELYAQSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10701..11777)
                     /gene="prfA"
                     /locus_tag="WJL87_000640"
     CDS             complement(10701..11777)
                     /gene="prfA"
                     /locus_tag="WJL87_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ7799105.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGELDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIVGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PADLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(11867..12637)
                     /locus_tag="WJL87_000641"
     CDS             complement(11867..12637)
                     /locus_tag="WJL87_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ7799106.1"
                     /translation="MRLTDTHCHLADSVLRGNLAQVLAEARQAGVWRFIVPATRPQDW
                     QSVAELSEMPSEHGQIRIALGIHPWFSDGIAERDCVRLETMLARYPQAWVGEIGLDFY
                     DKTQTPPQRERQIQVFVRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANILRIAEIAAQIRGTGAAEIAAITERNADSLLRP"
     gene            complement(12640..13632)
                     /locus_tag="WJL87_000642"
     CDS             complement(12640..13632)
                     /locus_tag="WJL87_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ7799107.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGTLIDSSAVQLQNWCAWLDIIATALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     DKPIVLTGSQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGALAEWDETQGWHNVRIPNQDTADVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAHALILQSYGHGNTPADEGFIRAVQDFTQQGRLLLNISQVRQGR
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSEGWGADEVKHELQRLGLV"
     gene            complement(13699..14382)
                     /locus_tag="WJL87_000643"
     CDS             complement(13699..14382)
                     /locus_tag="WJL87_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ7799108.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLVGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14571..15905)
                     /gene="glmM"
                     /locus_tag="WJL87_000644"
     CDS             complement(14571..15905)
                     /gene="glmM"
                     /locus_tag="WJL87_000644"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ7799109.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEIELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLAQLNQRSWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIASAIGSL"
     gene            complement(16035..16886)
                     /gene="folP"
                     /locus_tag="WJL87_000645"
     CDS             complement(16035..16886)
                     /gene="folP"
                     /locus_tag="WJL87_000645"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ7799110.1"
                     /translation="MARHGWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAKTA
                     LAHAERLLKEGADILDIGGESTRPGADYVSSEEEWARVEPVLVEVAGWGVPVSLDTRR
                     TVVMEKALALGGIDIINDVAALTDEGAVELLARQTDTGICLMHMQGLPETMQINPKYQ
                     DVVGEVARYLKARAAECIAAGIAPQRITLDPGFGFGKTLQHNIALMRHLPELMAETGF
                     PLLIGVSRKRMIGELTGEADAAARVHGSVAAALASVARGAQIVRVHDVKATADALKAW
                     EALGINL"
BASE COUNT         3729 a         4740 c         4544 g         3899 t
ORIGIN      
        1 tcccataaaa aaacggagca gatacctgcc ccgtttttat ttaatccgaa attttaatct
       61 aaatttagaa ttttgcaccg gattggtttg ccatatagtc aacagccgct ttgacttcgt
      121 catcgctcaa accggcattg ccgcctttgg ccggcatcgc gttaaagcct tcaagggcgt
      181 gtttgtgcaa ggtttctttg ccttttttga tacgcggtgc ccaatcgtct tttttgccta
      241 tgccgggaat accgggaatc gaaccgccgt ggcacacctg acaggttgct tcgaagactt
      301 ttttaccgtc aacgccgacc gcaggggctg ccgcaccctt gtcttctgcc ttcgcttctg
      361 ccggagctgc actatcggca ggagcagaag ctgttcctga agcggcattg tcggcaggag
      421 cagcctcatc aggattcggg aaagaaccgc cgcttttgtt cgccatgtaa gtaatcgccc
      481 gtttgagttc ctgatcggtc aggtctgccg caccgccttt ggcaggcatg gcgttaaagc
      541 cgttcagcgc gtgttggaac aaggtatcga agccttgcgc gatacgcggt gcccaatcgc
      601 cgttgtgttc cagtttcgga gcgttcggca cattgctgtc cgccgcgtgg cattggatac
      661 agattttgcc gaaaatctgt tcgccttggc gttcgccgac ggggatgccg tcgcccatcg
      721 tcaattgtcc gacaggctgg atacgggtct gcgttgctgc ttccgtagtg gcatcgacat
      781 cgccgaacga gccgctgccc gccagcttaa tcaggaaata aaggactgca ataacaataa
      841 cgataccgct cacaagggta aacagtgcag agccttgggc tttgttgtcg cggagttgtt
      901 tcatttggta ggcctcgccg tcaggttagg ttgtgctgta aattatagtt tggtgtgtta
      961 aacgcagtta acaatatttt gctggattat actgaattca cagggtcttt ccaatcgcta
     1021 tcattgaaaa tatgaaaaaa tttgccaacg gtatctgtat aaaacaaata atcctttgaa
     1081 aataatggtt tatcctcaag aaaactctcc ttatgccgcc atacgccgcc tgccggcgca
     1141 agataacctt tgccaatttg cagaatttac gttaacctta cgttttacgc acccatagct
     1201 cagttggaag agtgtcagtt tccgaagctg gaggtcacag gttcgatccc tgttgggtgc
     1261 gccaattata aagagaccgt ctgaaagata aatatttttc agacggtctt ttgacttact
     1321 tcaaactctt atttcaagac ttccgcaaat gcgcgggcaa catagtcggt attcgacgta
     1381 ttcagtccgg cgacgcacat cctgccggaa tccagcaggt aaacggcaaa ttcgtcgcgc
     1441 agcctgcgga cttgttccac gctcaatcct gtgtagccga acatgccgcg ctgtttgatg
     1501 aaataagtga aatcgcgatc ggggatttgc gcagttaaga catcataaag tttctgccgc
     1561 atcgcacgga tgcggtcgcg catcatataa acctcgtttt gccacaaggc gtaaagttcg
     1621 gggctgttca tcacgtcggc ggcgatatac gcgccgtgcg cgggcgggct ggagtagatg
     1681 cggcggacgg tgaatttgag ctgtccgaac accaaatccg cttcttcctt attcgggcaa
     1741 accacgctca ggccgccgac gcgctcgccg tagagcgaca ggttttttga gaaggaattg
     1801 ctgacgaaca agggcaattc catttccacc gctttgcgga cggcgtaggc atcgctgtcc
     1861 aaatcgccgc cgaagccttg gtaggcgatg tccataaacg gaatcagttt gcgcgttttg
     1921 atgatgtgca acacttcgtc ccattgttgt tccgacatat ccacgccggt cgggttgtgg
     1981 cagcagggat ggaggatcag gacgctgttt tcgggcaggg tgttgaaaaa cgcggtcatt
     2041 tcgtcgaatt tcacgccgac ggcagcaggg tcgtaatatg ggtaagtgcc gacctcgaaa
     2101 cctgcgcttt caaaaatgcc gcgatggttg tcccaagtcg ggtcgctgac gtaggcgcgc
     2161 gcttcgggaa accagcggtg cagaaagtcc gccccgactt tgagcgcgcc tgagccgccc
     2221 aaggtttgta cggtagcgat gcgtccttgc gcgagcgcgg ggttgtcttt gccgaacaac
     2281 aaatgctgca ccgcgctgcg gtaagtgtct aagccttcca tcggcaggta gggcgacggc
     2341 acaggagtgg cggcgcgggc ggtttcggcg cggctcacgg attccaacac gggcattttg
     2401 ccttcgtcgt cgaaataaat gcctatgctc aaattgactt tttcggggcg cgggtcgttt
     2461 ttgaaggttt cgaccaaact caaaatcgga tcgccgggat agtattcgat gtgtcggtac
     2521 atagtcctta cctcttgctt tttccaagga ttttcttttt caacagcaca ccgctttcaa
     2581 tatggtgcgt aaacggaaat tgatcgaaca gggcggcacg ttcaaccgta tgggtttccg
     2641 ccaaggtgtc caaattggta cgcagcgttt cgggattgca ggaaatgtag atgatgttgt
     2701 caaactgcga caccagcttc aaagtttcct catcgatacc ggcacgcggc ggatcgacga
     2761 aaatagtgga aaatgcgtaa tccgtcaaag caataccgcc atccttaagg cgtttaaact
     2821 cacgttttcc ggtataggct tcggtaaatt cttcagcaga cagacgggcg attttgatgt
     2881 tgccgatgcg gttggcttcg atattccatt gcgccgcgct gacggaggtt ttggagattt
     2941 cggttgccaa aacacgttca aacttttcag ataaaggaag cgtaaagttg ccgtttccgc
     3001 aatacagttc gagcaaatcc ccatccaatc cttctgctgc accgcaggcc cattcaagca
     3061 ttttctgaca cacggcggca ttcggttggg taaaactgcc ttcaatttgc cgataacgga
     3121 aatcccggtt gccgaccttc aaagtttccg ttacatagtc ctgttttaag actattttct
     3181 gtcccctgct ccgcccgatg acggaaatat ccaactgttg ctgtaacgct tgcgccgcct
     3241 gcatccactc attatcaagc cttttgtggt aaatcatggt aaccagcatt tccccgctga
     3301 gcgtagacaa aaattcgacg gcataccaac ggtttctcag ttcggcagat tgggcggcaa
     3361 cggcaatcaa ttctggcatc aggcggttga cggcttcgga agccgcttca aaacggtcgc
     3421 aacggatcag gcttgccccg ccagcctttt gtcctttttc aaacatggca taaaacattt
     3481 caccgccttc gtgccaaata cggaactcgg cacgcatacg gtaatgtttg tccggagatt
     3541 cgtacacttc ccactcagga acatccaaac ctgcaaaaag ggttttaagg tggtcttttt
     3601 tatcttgaag ctgctgcgtg tagtcgctca tatcgctatc ctgaaaaatc agaccggatt
     3661 ataagccgat tcactgcaaa acacgcaacc cctttaccgg catataaccc gttttcctcc
     3721 aacttttatg ctaaaatccg ccgtctccaa cataccgccg acagaaggaa aatatggcag
     3781 gcaacacttt cggacaactc ttcaccgtga ccacgttcgg cgaaagccac ggcgcaggtt
     3841 tgggctgcat catcgacggc tgcccgcccg gcttggaatt aagcgaagcg gatatccaat
     3901 ttgacctcga ccgacgcaaa cccggcacca gccgccacgt tacccaacgc cgcgaaaccg
     3961 accaagtaga aatcctctcc ggcgtattca aaggcaaaac caccggcacg cccatcgccc
     4021 tcttaatccg caataccgac cagcgcagca aagactacgg caacatcgcc caaagtttcc
     4081 gccccggcca tgccgactat acctattggc acaaatacgg cacgcgcgac taccgcggcg
     4141 gcggcaggag ctccgcccgt gaaaccgccg cccgcgttgc tgccggagcc gttgccaaaa
     4201 aatggttgaa agaaaaattc ggcacggaaa tcaccgccta cgttacccaa gtcggcgaaa
     4261 aagaaatccg gtttgaaggc tgcgaacaca tttcccaaaa tccttttttt gccgccaacc
     4321 atagccaaat tgccgagctg gaaaactata tggacagcgt gcgcaaatcg ttggattccg
     4381 tcggcgcgaa gctgcatatt gaagccgcca atgtccccgt cggcttgggc gaacccgttt
     4441 tcgaccgcct cgatgccgaa atcgcctacg cgatgatggg catcaacgcc gtaaaaggcg
     4501 tggaaatcgg cgcaggtttt gacagcgtaa cgcaacgcgg cagcgaacac ggcgacgaac
     4561 tgaccccgca aggcttcctg tccaaccatt cgggcggcat cctcggcggc atcagtaccg
     4621 ggcaagacat ctgcgtcaat atcgccatca aacccaccag ctccatcgcc accccgcgcc
     4681 gcagtatcga catcaacggc aaccccatcg aactcgccac gcacggcagg cacgacccct
     4741 gcgtcggctt gcgcgccgcg ccgattgccg aagctatgct cgccttggtt ttaatcgacc
     4801 acgccctgcg ccatcgtgcg caaaatgcgg atgttgccgt ggatacgccc gatatcgccc
     4861 gctcggacaa gtaaaaagat ttagccaaaa cacgggcttt atactaaaat accgaccatt
     4921 gccgtgccgt ctgaacgcac gattcttaga ggaatacaac cgaaatgaca caagaaaccg
     4981 ctttgggtgc ggcactgaaa tccgccgtcc aaactatgag caaaaagaaa cagaccgaaa
     5041 tgatcgccga ccacatctac ggcaaatacg atgtgttcaa acgcttcaaa ccgttggcac
     5101 tcggcatcga tcaggatttg attgccgctt tgccgcaata cgatgccgca ctgattgcac
     5161 gcgtcctcgc caaccactgc cgccgtccgc gctatctgaa agccttggcg cgcggcggca
     5221 aacgtttcga tttgaacaac cgtttcaaag gcgaagttac ccccgaagaa caggcgatcg
     5281 cgcaaaacca tcctttcgtg cagcaggctt tgcaacagca gtccgcccaa gctgccgccg
     5341 aaacgccgtc tgttgaagcc gaagcagccg aatcttccgc agcagaataa atccccaaat
     5401 gaaatgccgt ctgaaaaccg atttggtttc agacggcatt tttccgtatg cggcaatcac
     5461 ggttcaaata tcgagttccg ccgtatcgcc ttcgcgctcc atccaggcac ggcgggcggc
     5521 ggcttcgcct ttgcccatca gtttgacgaa gatgtcgcgc gtctcgtcat ccgcgccttc
     5581 ggggatttgt acctgcaaca ggcggcgggt gtcggggtgc atggtagtat ctttgagctg
     5641 gtctgggttc atctcaccca aacctttgaa acggctgatg gaataagcag tttctttaac
     5701 gccttctttt tgcagccgct ctaaaatgcc gtcaagttcg ttttggtcga gggcgtagaa
     5761 tttgcgggcg ggtttgcttt taccttgtgc gttgacatct acgcggaaca gcggcggctg
     5821 ggcgacgtag atgtgtccgt cagcgaccag tttcgggaag tggcggtaga acagggtcag
     5881 cagcaaaact tgaatatgcg agccgtccac gtcggcatcg gacaggatgg cgattttgcc
     5941 gtaacgcagg ccgcttaaat cgggatggtc gttgatgccg tgcggatcga cgccgatggc
     6001 aactgaaatg tcgtggattt cggcgttgcc gaagagctgg tcggggtgga cttcaaagct
     6061 gttgagcact ttgccgcgca ggggcaggat ggcttgggtg gctttgtcgc gggcgagttt
     6121 ggcggagccg ccggcggaat cgccttcgac gaggaagagt tcgttttcgc ggatgtcttc
     6181 gctttcgcag tcggtcagct taccgggcag gacggcgacg ccgctgcctt ttttcttttc
     6241 aatcttttta accgaacgca tccgcgcctg tgcctgacgg atggcgagtt cggcgatttt
     6301 tttgccgaag tccacgtttt ggttcagcca caattccaaa gggtcgcccg atacggtagc
     6361 aacgagtttc agcgcgtcgc ggttggtcag tttgtctttg gtctgacctt ggaactgcgg
     6421 gtcgaggaca cgggcagaga ggacaaaggc ggttttgccg aacacgtcgt cgctttgcac
     6481 tttcacgccg cgtggtaaaa ggttgtgcag attgatgaag ttgctgacgg cgttgaacac
     6541 ggcttgtttc aagcccgctt cgtgcgtgcc gcccagcggg gtggggatga ggttgacgta
     6601 gctttcgttg gcgcacgagc cttcttccag ccaggtcagg gcaaacgcgg cgccttcgcc
     6661 gatgctgaaa tcgccgttgt ggccgtctga aatgtagttt tcgcaagaga agataggcac
     6721 ggcttcctgc gcgtcggcaa tcaggtcggt cagatagctt ttcagaccgt cggggtaatg
     6781 ccaggtttgg gtgtgcgctt cgtcttcgcc tttgaccgga cgggtcaggg aaacgcgcac
     6841 gcccggcagc agcacggctt tggcgcgcag caggcgttcg agttcgggga tgctgtaatt
     6901 cgggctttca aaatatttgc cgtccggcca gacgcgcact tcggtgccgc tgtctttgac
     6961 ggcgcatttg cccacttgtg ccaacggttc gaccacgtcg ccgccggcaa acacgatgcg
     7021 gtggattttg ccttcgcgtt tgaccgttac ctcaaggcgg gtggaaaggg cgttggtcac
     7081 ggatacgccc acgccgtgca ggccgcctga aaaggcatac gcgctgccgc cgtctttttt
     7141 gttgaacttg ccgcccgcgt gcagacgggt gaacacgagt tcgactacgg gtacgccttc
     7201 ttcagggtgc agaccgacgg gaataccgcg gccgttgtcg cgtacggaaa gcgaaccgtc
     7261 ttcatgaatt tgcacgtcga ttgccgtagc gaaaccgccc aacgcctcgt ccgccgcgtt
     7321 gtcgatgact tcttggcaga tatgggtcgg gctgtcggtg cgggtgtaca taccgggacg
     7381 ttctttgacc ggttccaaac ctttgaggac ggtgatgctg gattcgctgt attggttgtt
     7441 tttagccata ggaataatct gaaagtaaga aaaacaacgc tttcagacgg cctgaaagcg
     7501 ttgcgttccg ttgttttacc ggttgccgga aagttggcgg gcggcaaagt cttcataact
     7561 ttccataccg cgcaggaagc gggaagaaag ttctttcaat gcccccaaat aaacgtcgcg
     7621 tttgaaatca atcacttcgt caacgggcgc ccaatattgg tgccaacgcc agccgtcaaa
     7681 ttcggggtgg cgggtggcgc gcaggttgac atcgcaatcc cggccggtca ggcgcaggag
     7741 ataccaaatc tgcttctgcc cgcgataaga gccgcgccat tcgcggcgta cccagttgtt
     7801 cggcacgtcg taacgcagcc agtcgcgcgt ccgcccgacg attttgacgt gttgcgacag
     7861 aagtccgact tcttcgtaaa gctcgcggta catcgcggtt tcggggcttt cgcccggctt
     7921 gatgccgcct tgcggaaact gccaagaatg ttcgcgcacg cgcttacccc aaaagacttc
     7981 gtcgcggttg ttggttaaga tgataccgac attggggcga tagccttccc tgtccaacac
     8041 ggtgtcgccc tccgttaaat tcaatcttgg gattttccca caaatcgaac ggttttgaca
     8101 aatcagacgg tatggttcaa gcatgtcgaa acacagacgg actcgggaaa atatcttaaa
     8161 tttggtttac aataatgtgt ttcaaattat tcgggaatca gaccatgtta gacatccaat
     8221 tgctccgcag caacaccgcc gccgttgccg aacggcttgc acggcgcggt tatgactttg
     8281 ataccgcacg ttttgacgca ctggaagaac gacgcaagtc cgttcaggtg aaaaccgaag
     8341 aattacaagc ctcgcgtaac agcatttcca agcaaatcgg cgcactgaaa ggtcagggca
     8401 aacacgaaga ggcacaggcg gccatggatc aagtcgccca aatcaaaacc gatttggaac
     8461 aggcagccgc cgatttggaa gccgtgcaaa aggaactgga cgcatggctg ttgagcattc
     8521 cgaacctgcc gcatgaggac gtgcctttcg gtaaggatga aaccgaaaac gtcgaagtcc
     8581 gcaaagtcgg caccccgcgc gaatttgact ttgaaatcaa agaccatgtc gatttgggcg
     8641 aacctttggg tttggatttc gaaggtggcg cgaaactttc cggcgcacga tttaccgtga
     8701 tgcgcggaca aatcgcccgt ctgcaccgcg ccttggcaca gttcatgctg gatacgcaca
     8761 cgctgcaaca cggctacacc gagcattaca cgccttatat cgttgacgat acgacgctgc
     8821 aaggtacggg ccaactacca aaatttgcgg aagatctgtt ccacgttacc cgtggcggcg
     8881 acgaaaccaa aaccacccaa tacctgattc cgacagccga agttaccctg accaataccg
     8941 ttgccgacag cattatcccg tccgaacaac tgccgctgaa gctgaccgcg cattcgccct
     9001 gtttccgcag cgaggcgggt tcgtacggca aagacacgcg cggtctgatt cgccagcacc
     9061 agttcgacaa agtagaaatg gtacaaatcg ttcatcccga aaaatcatac gaagcactgg
     9121 aagaaatggt cggccatgcc gaaaacatcc tgaaggcttt ggaactgccc taccgcgtga
     9181 ttaccctgtg taccggcgac atgggcttcg gcgcaaccaa aacgtatgac ttggaagttt
     9241 gggttcccgc gcaaaatacc taccgcgaaa tctcaagctg ctccaactgc gaagatttcc
     9301 aagcccgccg catgaaggcg cgtttcaaag acgaaaacgg caaaaaccgc ttggtacata
     9361 ctttgaacgg ctccggcttg gctgtcggca gaacgctggt cgccgtattg gaaaaccatc
     9421 aaaacgccga cggcagcatc aacatccctg ccgcactgca accgtatatg ggcggcgttg
     9481 ccaagttgga agtgaaataa gtttgcaagc tgcctgaacg tcaaatgccg tctgaaacct
     9541 ggttcagacg gcatttcctt taaactttta aaacacgtca gccgtcggca cgaaccgcat
     9601 tgccgcaatc gccggtctgt ccgacctcgc ggatattgga cagcgtaact tccgaaatat
     9661 tacccaacgc ctcttccgtc aaaaacgcct gatgaccggt aaacagcacg ttatggcagg
     9721 acgacaaccg gcggaacacg tcgtcggtaa tcacatcgtt ggatttgtct tcaaaaaaca
     9781 gctcgcgctc gttctcgtac acatccatgc ccaatgcgcc gattttccgg cgtttcaacg
     9841 cctcaatcgc ggcggcactg tcaatcagcc cgccccggct ggtgttgata atcatcacgc
     9901 cgtctttcat tttgtcgaac gccgcttcgt tcagcatata gtggttttcc ggcgtggcgg
     9961 ggcaatgcag cgtgatgatg tccgactggg catacagctc gtccaaatcc acatacctgc
    10021 cgccgatttt ttccgcttcg gggttgcaaa acggatcgtg agccagcagg ttcatgccga
    10081 aaccctttaa aatccgcatg gttgcaatac cgattttccc cgtgccgata acgcccgccg
    10141 ttttgccgta catattgaaa ccggtcagac cttccagcga aaaattcgca tcgcgggtac
    10201 gctgataggc tttgtggata cggcggttca aagtcagcat cagaccgacc gtatgttccg
    10261 caaccgattc gggcgaatag gcaggcacgc gcacgacttt caagcccaac tcttcagccg
    10321 cctttaaatc cacattattg aagccggcac aacgcaacgc cacagttttc acgccaattt
    10381 gcgccaattt ttccaacacg ggccggctgc cgtcgtcgtt tacaaaaata caaaccgctt
    10441 ccgcgccttc cgccattttc gccgttttcg catccagcat aaaatcaaaa aactccagct
    10501 cgaagccgaa atgccggttg gcgcgggtaa aatgttcgcg gtcatagctt ttcgtaccgt
    10561 aaatcgcaat cttcatcaat atgtccagtt gtcgcctatg gttgagaaac ggcataatac
    10621 actgaattca aacaaatcag tagaatatgg cagattaaaa ttgattgcat gcacggcatt
    10681 tccatttcaa aacacaaaac tcaatcgccc attgccgcca aaagctccgc ctgatgttcg
    10741 gcaatcaggg catcggtgat ttctgccaaa tcgccgtcca tcacaaaatc cagcttgtgc
    10801 agggtgaggt tgatgcggtg gtctgttacc ctgccttggg gatagttgta ggtgcggatg
    10861 cgttcgctgc ggtcgccgct gccgatgagg gatttgcgtt cggcggcttc tttggcttgg
    10921 gcttcgcgtt tttgcgcgtc gttcaggcgg gcggcgagga ctttcatcgc ctgcgctttg
    10981 ttggcgtgtt ggctgcgtcc gtcttggcat tcgaccacca tgcctgtggg caggtgggtg
    11041 atgcggacgg cggaatcggt tttattgatg tgctgcccgc ccgcgccgga tgcgcgaaag
    11101 gtgtcgatgc gaaggtcggc tgggttcagt tcgatgtctt ccagttcgtc cgcttcgggc
    11161 atgacggcaa cggtgcaggc ggaggtgtgg atgcggcctt ggctttcggt ggcggggacg
    11221 cgctgcacgc ggtgtccgcc cgattcaaat ttcaaacggc tgtacgcccc gagtccgaca
    11281 atacgggcga tgacttcttt atagccgccc aattcgcttt cgttggcgga cacgatttca
    11341 acctgccaac ggttgcgctc ggcgtagcgg ctgtacatac gcagcaaatc gccggcaaac
    11401 agcgcggctt cgtcgccgcc cgttccggcg cgtatttcga tgaagatgtt tttgtcgtcg
    11461 tcggcatctt tgggcagcag cagtttttgc agttcggtat cgagttcgcc gattttggct
    11521 ttggccgctt cgatttcttc ggcggcaaaa tctttcattt cggggtcgga cagcatttct
    11581 tcggcatctg ccaagtcgct ttgggcaagc cgatagtttt ggaatacttc gacgacgggg
    11641 gtcagttcgg cgtgttcgcg cgtgagcttg cggtagttgt ccatatcgga tgtggcttcg
    11701 ggttgcccta aaaggtgggt aacttcttcc aaccggtcgc cgagttgttg cagtttttct
    11761 aagatagacg gcttcataat tcttccataa caaacgccgc ctgaatttca gacggcatca
    11821 aaccgcggtc attataatat attttctgaa tattcaaaaa gataatctag ggacggagca
    11881 ggctgtcggc attgcgttcc gttatcgcgg cgatttccgc cgcgccggta ccgcgtatct
    11941 gcgcggcaat ttcggcaatc cgaagaatgt tggcaggcgt attaatctct tttttcagca
    12001 taaacgggct gtcggtttcc aatacgaaat ccccgtcgtt caaggcttta agcgtatcgc
    12061 gcactttacg cgcgttcgga ttgagcaaca acgaaccgat gccgattttg aaacccagtt
    12121 tcgtcaacac gcgcgcttct tccgcgctgc ctgagaaggc gtgaacgatg ccgccttggg
    12181 caaagcctgt ctgtttgacg gcggcggcga tgtcggcggt ggctttgaga ttatggataa
    12241 tcacgcggcg gcgcagggtt tgcgcgatgg caagctggcg gacgaaaact tggatttgcc
    12301 gttcgcgctg cggcggcgtt tgggttttgt cgtaaaaatc caagccgatt tcgccgaccc
    12361 atgcctgcgg ataacgtgcc aacatcgttt ccaggcggac gcaatcacgc tcggcaatgc
    12421 cgtctgaaaa ccaaggatgg atgccgagcg caatacggat ttgaccgtgt tcggacggca
    12481 tttccgacag ttccgccacg ctttgccaat cctgcgggcg cgtcgcggga acgataaacc
    12541 gccacacacc cgcctgtcgc gcttcggcaa gcacctgcgc cagattgccg cgcaaaacag
    12601 aatcggcaag atggcaatgt gtgtcggtca gcctcatctt cacaccagcc ccaaccgctg
    12661 caactcgtgt ttcacctcgt ccgcgcccca tccttcagaa acggcaagcg tcatcaatgc
    12721 ggttgcggtt tccaaattgc atttgccgcc gttgatgatg cccgagttgc ggaacgcatt
    12781 gccttgcgcg taaacggcgg cagttctgcc ttgtcggact tggctgatgt tgagcagcag
    12841 cctgccttgc tgcgtgaagt cttgcacggc gcggataaag ccttcgtctg ccggggtgtt
    12901 gccgtgcccg tagctttgca ggataagggc gtgggcggga agctgtccga gtccgtccgc
    12961 aagttcttgg acggcaaagc cggggataag cgtgcggaca gcgatttttg cctgaggatc
    13021 gggataacgg attttgaggc cgtctgaaac atctgccgtg tcctgattcg ggatgcggac
    13081 attgtgccag ccctgggttt cgtcccactc cgccagtgcg ccgaaatgcg gattgtcgaa
    13141 accggcggcg gtttcggtgc tgactttgct gctgccgacg gcgggataaa gtttgccgtc
    13201 aaaggcaata agcgtctgct tcaatccgag tgggaatgcg gcaacggcag tcgccagatt
    13261 gcgcggcgcg tcgctgccca cggcgttgta cggccattgg gagccggtca ggacgattgg
    13321 cttgtccaaa ccctgcagcg cgagggcgaa cagattggcg gtataggcga gcgtgtccgt
    13381 gccgtgcagc accaaaacgc cgtcgtactg cggcagcgcg gtggcaatga tgtccagcca
    13441 agcgcaccag ttttgcagtt ggacggcgga agaatcaatc agcgtcccac aaacgtgcca
    13501 atcgaaatcc atgccgtttg aaaacggcgc gagtgcctgc ccgaccagcg cggtatcggg
    13561 gcgcaggcct tcgctgcttt gcgtcatgcc gatggtgccg ccggtataaa ggacaaaaat
    13621 tttctgtttc atggctcatc ctgaaaatgc aaagaccgcc tgaaacgcga aatgcgtccg
    13681 gacggtcgaa aatctgattt actgctgctt ctgtgccgct tttttcgctg ccttttccgc
    13741 cttgcgtttg gcgcgttttt cgctcaattg tgcgcggtaa agctgataat gtcggcgttt
    13801 gcgccaccag aaccacgcca gcgccgccgc cagcacgccc aatgcgatga agatgccgga
    13861 ttgcaggctg tgcattttcg ccatcagcca atcgatgttg tgcgcgccgt actcgcccaa
    13921 gtaaatccaa acgggcacgg aaatcagcgc ggcaagcccg tccataatca gaaagcgcag
    13981 atacgatact ttgcggctga tgccggcggt aacgaaaacg gcagtccgca aacccggcag
    14041 gaaacgagcg acaaacagaa cccagttgcc gtatttgtcg aatttttcct gaacctgtgc
    14101 gtaacgtttc ggcgtcatga tgcgcgcaat cggtttgaac ttgaggattt tctgccccca
    14161 gatgcgtccg gcggcgaaca tgatgccgtc cccgaccaat acgccgagca taccgactgc
    14221 aaacataata tgcggattgg tataacccat acccgaaatc acgccgcctg ttaccaaggt
    14281 caaatcctcg ggaatcggca cgccgaaacc gcagatgacc aaaacgaaaa acacggccgc
    14341 atagccgtat tcgacaaaaa aggcttccaa aagggcaaac atagcggata ttccattgtc
    14401 ggagataaaa agtcagaaca agccgaaaca ttttctgcgc gaaacggcat tctatcaaag
    14461 attatgccgt ctgaaagcgg caaggggcag attcgcgccc tctgccggac gttcagacgg
    14521 catttaaaca ggaaaaccgc cgctgttgaa acggttttgc ctgattttgc ctaaaggctg
    14581 ccgatggcgg aagcaatacg ttccgcaccg tcgcgcgccc aatccgcctg ccgcgcctcc
    14641 accatcacac gcacgacggg ttcggttccc gaagcgcgca acacgacacg ccctttgcct
    14701 tcgagttctt tttccacttc cgccaacacg tcttttgagg cttcctgcca tttctgacct
    14761 ttttggatgc gcacgttaat catcgtttgc ggatacggct gccaatcggc gcaaacggtg
    14821 gcgaggtctt ggttcagcgt ttgcagtgcc gccaaaactt gcagcgcgga aataatgccg
    14881 tcgccggtgt tgtgtttgtc catgcacaaa atatggccgc tggcttcgcc gccgatgagc
    14941 cagctgcgtt ggttcagctg tgccaacaca tagcggtcgc cgactttggc gcggcagaaa
    15001 tccacgccct gctctttcag ggcgatttcc atcgccatat tggtcatgac cgtgccgacc
    15061 acgccgccga tgttgatacc ttctcgggcg cgggctttag caatgacgta aatcaggctg
    15121 tcgccgtcgt aaaccttacc gtttttatcg accatcatca ggcggtctcc gtcgccgtct
    15181 aaggcgatgc cgtagtcggc ttcatgctgt aaaacggctg cctggagtgt cttggtataa
    15241 gtcgcaccgc atttttcatt gatattgtag ccgttgggtt cgtcgccgat gctgacgact
    15301 tgtgcgccca gttcgtgaaa caccttgggt gcgacaccgt accccgcgcc gttggcagta
    15361 tcgataacca acttcaaacc ccgaaggtcg gaatggctgg gaaaggtaga tttgcaaaat
    15421 tcgatatagc ggtcgtccgc accactgatg cggcgtgcgc gaccgaggcg tgcagaaggc
    15481 tgggttttca tttcttcgtc gattttggct tcgatttcca actcgatttc atcgctcagt
    15541 ttcacgccgc cctcggcaaa gaatttgatg ccgttgtcgg aataggcgtt gtgcgacgcg
    15601 gaaatcatca cgccggcgga caggcgcaac gcgcgggtca gataagccac gccgggcgtg
    15661 ggcagcggtc cggtctgtac cacattcaca cccgccgccg taaaaccggc caccaaagcg
    15721 gcttccagca tatagccgga aatgcgcgtg tctttgccga tgaggacggt cggtttctgg
    15781 tcggtgtcgt gctgcaccaa aacctgcccc gccgcatagc cgagtttcaa tacgaaatcg
    15841 ggcgtaatcg gaaattgccc cacttcgccg cgcacgccgt ccgtgccgaa atattttttt
    15901 gccatgtgtt gctccgagaa tgtggaccgt tgtccgagat tatacagtca gtttatgcct
    15961 tgctgtctgc accgttgatg ccgtctgaag ccgccccgtc cttttcagac ggcatgaagt
    16021 atgcgaaccg ctgtttacag attgatgccc aacgcttccc atgccttcaa cgcatccgcc
    16081 gtcgccttca catcatgcac ccgcacgatt tgcgcgccgc gcgccacgga agccagtgcc
    16141 gccgccacgc tgccgtgtac gcgcgccgcc gcgtctgcct cgccagtcag ctcgcctatc
    16201 atacgtttgc gcgacacgcc gatgagcagc ggaaaacccg tttccgccat caattcgggt
    16261 aaatgccgca tcagtgcgat attgtgttgt aaggttttgc cgaaaccgaa accggggtcg
    16321 agtgtgatgc gttgcggggc gatgcctgcc gcgatgcatt ccgccgcccg cgctttcaaa
    16381 taccgcgcca cttcaccaac aacatcctga tatttcggat taatctgcat ggtttcaggc
    16441 aaaccctgca tatgcatcag gcaaataccc gtgtccgtct ggcgcgccag caattcgacc
    16501 gcgccttcgt cggtcaacgc cgccacatcg ttaataatat cgatgccgcc gagtgccaac
    16561 gctttttcca taaccaccgt gcggcgcgtg tccaaactga cgggaacgcc ccatcccgcg
    16621 acttctacca atacaggctc aacccgcgcc cattcttctt cggacgaaac ataatccgca
    16681 cccggccgcg tcgattcgcc gccaatgtcg agaatgtccg caccctcttt caaaagccgt
    16741 tcggcgtgcg ccaaagcggt tttggcattt tgcgaatacg cgccgccgtc ggaaaaagaa
    16801 tcaggcgtga gattcacgat gcccataatt ttcggcttgt ccaaaccgat ttcaaaccgt
    16861 cctgcctgcc aaccgtgtcg tgccatctga actcctccca aaataaaaaa ac
//
LOCUS       DAWKST010000028        16636 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.028, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000028 DAWKST010000000
VERSION     DAWKST010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16636
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.028"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(51..1220)
                     /locus_tag="WJL87_000646"
     CDS             complement(51..1220)
                     /locus_tag="WJL87_000646"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ7799111.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGLL
                     PWLRPDAKAQLTVVYDSESGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1402..1512)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1565..2461
                     /locus_tag="WJL87_000647"
     CDS             1565..2461
                     /locus_tag="WJL87_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ7799112.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2505..3569)
                     /gene="tsaD"
                     /locus_tag="WJL87_000648"
     CDS             complement(2505..3569)
                     /gene="tsaD"
                     /locus_tag="WJL87_000648"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ7799113.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALAFAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLKT
                     GREAGAFNVRPRWPLSEIVK"
     gene            complement(3685..4872)
                     /gene="ccsB"
                     /locus_tag="WJL87_000649"
     CDS             complement(3685..4872)
                     /gene="ccsB"
                     /locus_tag="WJL87_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ7799114.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEEKGGKSWLPQSVLIEEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4865..6817)
                     /locus_tag="WJL87_000650"
     CDS             complement(4865..6817)
                     /locus_tag="WJL87_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ7799115.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTVSREGGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILSASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7094..7717)
                     /locus_tag="WJL87_000651"
     CDS             complement(7094..7717)
                     /locus_tag="WJL87_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ7799116.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7923..8552
                     /locus_tag="WJL87_000652"
     CDS             7923..8552
                     /locus_tag="WJL87_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ7799117.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPKEN"
     gene            complement(8673..11069)
                     /locus_tag="WJL87_000653"
     CDS             complement(8673..11069)
                     /locus_tag="WJL87_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ7799118.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVQKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLEAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11221..12336
                     /locus_tag="WJL87_000654"
     CDS             11221..12336
                     /locus_tag="WJL87_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ7799119.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGITQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12339..12938
                     /locus_tag="WJL87_000655"
     CDS             12339..12938
                     /locus_tag="WJL87_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ7799120.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            12939..13586
                     /locus_tag="WJL87_000656"
     CDS             12939..13586
                     /locus_tag="WJL87_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ7799121.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13604..14149
                     /locus_tag="WJL87_000657"
     CDS             13604..14149
                     /locus_tag="WJL87_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ7799122.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDNSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14168..16453
                     /locus_tag="WJL87_000658"
     CDS             14168..16453
                     /locus_tag="WJL87_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ7799123.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSKPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQAAAPAKQT
                     NIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKT
                     PVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKT
                     FTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQ
                     ARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRS
                     ILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQV
                     MIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGAETKINL
                     PITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIP
                     FTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTIL
                     CISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDR
                     RELLIFITPRIMGTAGNSLRY"
BASE COUNT         4504 a         4733 c         3782 g         3617 t
ORIGIN      
        1 aaaatgccgt ctgaactgtt cagacggcat ttttgatttt caaaccggaa ttacagcccc
       61 gctgccgccc tcaatgcagc agctttgtcg gtgcgctccc aagtgaactc aggttcttcg
      121 cggccgaaat gtccgtaagc ggcggattta ctgtaaatcg ggcgcaagag atcgagcatt
      181 tggacgatgc ctttggggcg caggtcgaaa tgttcgcgaa ctaaggtaat cagtttttcc
      241 tcgctgattt tgcctgtgcc gaaagtgtcg atggaaatcg aagtcggttc ggcaacgccg
      301 atggcgtagg aaacttggat ttggcattgg gttgccaaac cggcggcgac gatgtttttc
      361 gcaacgtagc ggcaggcgta agcggcggaa cgatccactt tggacgggtc tttgccggag
      421 aacgcgccgc cgccgtgcgg agccgcgccg ccgtaggtat caacgatgat tttacggccg
      481 gtcaaaccgc agtcgccttg cgggccgccg atgacgaagc ggccggtcgg gttgatcagg
      541 tatttggttt cgtcggtcag cagttcagac ggcaggacgg gcttgataat ctgctcaatg
      601 acggcgtttt tcaactcttc ataaccgatt tccgggtcgt gttgcgtaga gaggacgacg
      661 gtatcaatgc gttttacttt gccggattcg ctatcgtaaa ccacggtcag ttgggctttt
      721 gcgtcgggac gcagccaagg caggaggccg tctttgcgta attcgctttg acgctgcatc
      781 aggcggtggc tgtaatagat ggcaaacggc atcagggtcg gggtttcgtc gcaggcatag
      841 ccgaacatca aaccttggtc gccagcgcct tggttcaagt caatgccttc gccttcgttc
      901 acgccttggg cgatgtccgg agattgctgg tcgtagtaca cgccgaccgc gcagccgttg
      961 gcatcaaagc ccagctcgga ggagttgtag ccgatgcgtt tgatggtttc gcgtgcgact
     1021 ttgatgtagt ccacttgggc ggtggtggtg atttcgcctg ccaatacgca caagcctgtg
     1081 ttgaccaaag tttctgcggc gacacgcgct ttggggtctt gcgccaagat ggcatccaaa
     1141 atcgcgtcgg atacttggtc ggcaactttg tccggatggc cttcggatac cgattcggaa
     1201 gtaaacagat attcgctcat tgagattttc cttgaaaaac aaaccatctt cttcagacgg
     1261 catgttgtat gaacataatg tcgacagcgg gaaatatagc aaaatttccc tattcatacc
     1321 attcagttga gaaatattcc catttgaatg gcactttgga atctctgccc gtacgttctt
     1381 acaggcaaaa aaattcccgc atcaagcggg tttggattgc tctggtgagc cacatcggct
     1441 tttcaaccgt ccaccttact tttccttttg aaaagcaggt tggcatggaa ttcccaactc
     1501 ttaatgcagc gcggatggta gcagaaaagc ctttccgccg caatacttcc ctttttcaga
     1561 cggcatgttt cgtttacaat tcgggctgtt tccccctttg cgaaccgcca tgcacatcct
     1621 gttgaccgcc ctgctcaaat gcctctccct gctgccactt tcctgtctgc acacgctggg
     1681 aaaccggctc ggacatctgg cgttttacct tttaaaggaa gaccgcgcgc gcatcgtcgc
     1741 caatatgcgt caggcaggca tgaatcccga ccccaaaaca gtcaaagccg tttttgcgga
     1801 aacggcaaaa ggcggtttgg aacttgcccc cgcgtttttc agaaaaccgg aagacataga
     1861 aacaatgttc aaagcggtac acggctggga acatgtgcag caggctttgg acaaacacga
     1921 agggctgcta ttcatcacgc cgcacatcgg cagctacgat ttgggcggac gctacatcag
     1981 ccagcagctt ccgttcccgc tgaccgccat gtacaaaccg ccgaaaatca aagcgataga
     2041 caaaatcatg caggcgggca gggttcgcgg caaaggaaaa accgcgccta ccagcataca
     2101 aggggtcaaa caaatcatca aagccctgcg ttcgggcgaa gcaaccatcg tcctgcccga
     2161 ccacgtcccc tcccctcaag aaggcgggga aggcgtatgg gtggatttct tcggcaaacc
     2221 tgcctatacc atgacgctgg cggcaaaatt ggcacacgtc aaaggcgtga aaaccctgtt
     2281 tttctgctgc gaacgcctgc ctggcggaca aggtttcgat ttgcacatcc gccccgtcca
     2341 aggggaattg aacggcgaca aagcccacga tgccgccgtg ttcaaccgca atgccgaata
     2401 ttggatacgc cgttttccga cgcagtatct gtttatgtac aaccgctaca aaatgccgta
     2461 acgaaaataa aaatgccgtc tgaacaattt cagacggcat tttgtcattt gacgatttcc
     2521 gacagcggcc agcgcgggcg tacattaaat gcaccggctt ccctacccgt tttcaaatgc
     2581 atcgcgcctg caaaggcaat catggcaccg ttgtccgtgc agtatgccgt cggcgggaaa
     2641 aacacgctga ctttttcgga cggatgtttc ggcttgcctt tgggggtcgg gatttgcacc
     2701 gtcatgttgc cgaaagtttc acggagtttg cggtttgcac cgaccccgcc ggcgaccact
     2761 acggttctga accctgtctg caacagggct tttttcactt tcgccgccaa cacatcgact
     2821 accgcatctt gaaacgcacg gcagatgtcg ttgcgtgtct gctcaggaat gtcatccgcc
     2881 ccgttttccg cgcgcacttt ctcgacggca gtcaaaacag cggttttcaa acctgaaaaa
     2941 ctcatctgca aatcgtcgga atgaatcatc gggcgcggaa aaacaaacgc ttcgggcctg
     3001 cccgattccg caagttccga cagtttcgca ccgcccggat acggcaagcc cagcagtttc
     3061 gccgttttgt cgaatgcctc gcccgccgca tcatcgacgc tctcgcccaa aagcgcgtag
     3121 tcgcctatgc ccctgaccgc cataatctgc gtatgcccgc ccgaaaccaa cagcgcgaca
     3181 aaaggaaagt cgggtttttc ctccgccaac agcggcgaca gcagatgccc ttccaaatga
     3241 tggacgggaa taacaggctt atccagcgca aaagccaagg cattggcgta actcgaaccc
     3301 gccagcaacg cgccgcccaa accgggcccc tgcgtaaagg caaccgcgtc aatgtcgcca
     3361 tacgatgcgc ctgcctgcgc cagacagcct tccgtcaacg gaacaaggcg gcggatatgg
     3421 tcgcggcttg ccagctccgg cacaaccccg ccgtattcgg cgtgcattgc catctgcgtg
     3481 tgcagacagt gcgcccgcaa tcctcgttcg gtgtcataca gcgcaacgcc tgtttcgtcg
     3541 caagaagatt cgattcccaa taccaacatg gtctgatgcc gttaaaaact gaaaaacgta
     3601 ttctagcgga tttcggcacg actgccgtat cccaaaaacg gaacatgccg tctgaagccc
     3661 ttcagacggc atcgtcgcac cgtatcaaag cgttccgtaa gaatgcagcc cgctcaaaaa
     3721 catattcacg ccgataaagg caaatgcggt tacgaacaaa ccgataatcg cccaccacgc
     3781 cagcactttg ccgcgccaac cggcaaccag ccgcaagtgc agccaaacgg cgtaattgag
     3841 ccagacgatg aacgcccacg tctctttcgg gtcccaactc caatagcgtc cccaagcatc
     3901 cgccgcccac agcgcaccca aaatggtggc aatggtaaag aacagaaagc cgacggcaat
     3961 cgccttatac ataacttcct caatcaacac ggattgcggc agccacgatt tcccgccttt
     4021 ttcttccgca cgcagggaaa ccagttcggc aataccgagc atcgcggaaa tgcaaaacgc
     4081 gccgtaaccg ataaagtttg ccggaacgtg gattttcatc caccaagatt gcaatgccgg
     4141 aatcagcggc tggatggtat gcgcctcgcg ggacacgctg taccacaaga caaatccaac
     4201 cacgaccgcc ataaagccga acacgaagcc gcccaatttc tgtatggcga acttaccttc
     4261 ataataaaga tacatcagcg cggtaatcac caaaaacagg atgaacactt catacaggtt
     4321 ggaaaccgga atatgccccg catcgggacg gagcagatag ctttcgtgcc aacgtaccag
     4381 cagaccggta aagcctgcta cggcagacac ccatgcaaac acggttccca tacccaacag
     4441 cgtgttggtc ggcacatttt ttacgcttgc caaaaccgcg cccgaaatat aggcgaacag
     4501 ggcgaagaag acaaaggcgc actgccacat aatcgccgac tggctgctga ggaaataccg
     4561 caacaggaaa atctctgccg atttgatgtt gccgccatac aaaccgacgg cggcataggc
     4621 aagcaatacg cttaaaggaa caaaccagcg catcggtttg aaaaaccaac ccaaaaacac
     4681 ggcaataccg gcacttgccc acaacatgac cgtttcgtaa atgtccatat gcataccgga
     4741 acgggtctgt acgaaaaccg tagccgcaaa aaccagcacg gcaaataccc aatcccaaag
     4801 attcagattg ctgatcaaag acttctgaat cagcagctcg tgttccggaa gggttttata
     4861 gtgttcagtc atgattcaag tccttgccga gccgttgcag actctcgacg tgttttggaa
     4921 attccttctg caaatcccgt tcgctgcggg ccgaagacat ggcaaaacgg attttgccgt
     4981 ctgaaaacaa tacccacgcc cgtttttcgc gcacataaaa catcaatacc gtacccaata
     5041 ccaacagcac cgagccgaga tagaccaaaa gcgcacccgg ggaacgggtc atctgcaaac
     5101 ccgacgaacg cacctcggaa aacccatcaa gttgcagcag cataggcgcg ggatattcgg
     5161 tcaaacccgt gtacgcatcc atactgtgca gcaggaaacg gttccgcgct tcatcctgct
     5221 gccattcggg caagccgtac cggcgtatgg tttcatccaa agcagcgttc atcacgccgt
     5281 aaagcatttc gtagaaataa ccctgcatct tatcctgctg ctctttcggg atattggacg
     5341 taataaattc gtccaatccc aaatagcctt tttgtgcaaa gatgttcagc gtgttttccg
     5401 cagccagcat gaattgttcg cggatttcgg caggtgcgtc tttggttgcg tcggcaacca
     5461 gacgtttgcg cccttcccca tctttcaaaa actcacgcaa tgccataaag gtgtccgctt
     5521 tcaactgctt gtccaagggg atacgcagcc agcggtattg ctgctgcaag ccgctgcgcg
     5581 tgccggtaat ccaaaaataa tcctgttcct gcaaaaccgg cagcatatag tttttatatt
     5641 cgaccgcctg ccctgccgca tcacggatac ggtaaacaat ggaagggccg atattggtgt
     5701 attttttacc ttcctgagta acggcgcgga catcgttcag cgtggatttc aggctttttt
     5761 cccgttccgc gccctcgctc atgtcctcca cattcataga agtgaactga tcgaactcaa
     5821 gacgatattt gtgtttgcca atttccaacg gaaactggtg tatggatgtt gccttcaaca
     5881 cgacaggctc gcgcgaagca tcacccaaat tccacgcctt gaatgtcaaa tccgaaccgc
     5941 cgtcggcaaa actcgcctga taaatcgtga tgccgtgcaa ggtcaaagga tggttcacgc
     6001 ggatggtgcg ctcgagtttc tcaccggttg ccttgtccgt cacttcaata tcgctggcga
     6061 aatcacgcgg catacccgta ttgtaaaaat cgatatggaa ttttttcagt ttgacttcaa
     6121 aaggcaagtc ctgaaccaat atcccgttgt cggcattcag gaaaaccaca tccgcactct
     6181 gcccctcgga aatattgacg ttgcccctaa atgagagatt ggacgcactc aaaatacttt
     6241 cgggcttgaa atccttggca taaaccgcct gattgtccgg aacaatccga ccggtcagca
     6301 tacccagttt caacagcagg ttactgtcta tcaacccgcc caggcaaatg acaatcaaag
     6361 caacatgggc aaagatatag ccccatttgt tcatcgtgcc ttttttggcg gcaatcagaa
     6421 ccgacccgcc ctcacggctg acggtttttc cctgaaaacc ctgcacctcc agataacgtt
     6481 tggcaacttc gggggcaatt tttacatcca acagcgaaga atggcgcatc gccgccagag
     6541 atttttcttt aaccttttcc cgaaaagact tcatttcgcg ccagaacggc ggcacattgc
     6601 gaatcaggca caaactggta gaaaccacca aaaacatcat gataacgaca aaccatgccg
     6661 aagcatagac gtcatacagt cccagaaaac caaaaatctg cgcccaaaaa ggtccgaatt
     6721 tgaccaaata atccgtctgc ggctggtttt gctgcaacac cgtaccgata accgatgcaa
     6781 tacccagcag actgagcaaa gcgaccgcaa agcgcatgga gctgaaaaaa gcgaaccacg
     6841 gacgggaaag aagtggggga gatctacggg atttactcat tgtgtgttta ttccgccatc
     6901 aggaatatgg gaaagcagaa ttgggcaaac agaaaacaac gtcccgattc tactgtcttg
     6961 atgcttttta tttcaagaca atgaagacag cctgcatcga ttccaacggt tgcgattgaa
     7021 aaaacttatc gcagaattgc ctgaagccgt ctgaaaactt ttcagacggc ctctaaaaca
     7081 gactattgcg gaattaacgc aaaccttgga taaagttggc gaccgctttc aaatcttctt
     7141 cagacatacg gtttgcaata tcttccatga tggtattttt acgctgaccg gacttgtagg
     7201 cattcatctg ttcaacaata tatgcctgat gctgaccgcc caaacgcgga taagcctgaa
     7261 tttcgcttcc gcctcccggc atacccgcac cgctcggacc gtggcaggac atacacgccg
     7321 gcactttttt atcgctcaaa ccgccgcgat agattttcgc acccaattcg ggattttcct
     7381 taggattggc ttcaccggat ttgggctgct gtttggcata gaatgcggat acgttcaaaa
     7441 tatcctgatc gctcaaattc attaccaccg gtttcatcac agctgccgaa ccgtgggtgc
     7501 gtttaccgtc gcggatgccg atagtttgat gatagatgta agcagtatgc tgtgccgcca
     7561 aacgcggata catcgcaatg ccgctgttac cgtctgctgc atggcaagcc gcacaaaccg
     7621 ttgcggcaac ctgtttgcct ttttccacgt ctgctttggg agaggcggaa accgcaccgg
     7681 cagccaaaac aaaggccaat aaagtcaatc gtttcatgga gtgctcctga ttacagcatt
     7741 ggataacgca acaatgctct ttttatattc aaatacggga tttttgaccc gattaaaacc
     7801 gatgattctg taaacgtgtt attctatact aaatttacat taaattacca ctgtgtttca
     7861 cataaaacca accgcatatt tttgctgtcg gacaaacggc ggcggaaaac aaggatatgc
     7921 ccatgaacct ttttcaaaac gccaaattct tcacgacggt caaccacctt aaagacctgc
     7981 ccgacacccc tctcgaaatt gcctttgtcg gcaggagcaa tgccggaaaa tccagtgcca
     8041 tcaataccct gaccaaccat gtccgtcttg cctacgtttc aaaaacaccc ggacggacgc
     8101 agcatatcaa cttcttcgag ctgcagaacg gcaattttat ggtcgatttg cccggctacg
     8161 gttatgccca agtccccgaa gcagtacgcg cacattgggt caatctgctc ggcgactatc
     8221 tgcaacagcg caaacagctt atcgggctgg ttttgattat ggatgcccgc catcctttaa
     8281 aagaactcga catccgtatg ctggactttt tccacacgac cggcagaccg gttcacatcc
     8341 tgctgtcaaa agccgacaaa ttatccaaaa acgaacagat aaaaaccctg tcccaagtca
     8401 aaaaactgct caaaccttat tccgacaggc aaaacatcag cgtacagctg ttttccagcc
     8461 tgaaaaaaca aggtattgac gaggccaacc gaactgtcgg aagctggttt gacgcagcag
     8521 atgccgccgc ttcctctcca aaggaaaact gaccccaatt atacggaaac cgtatttccc
     8581 ccacttgacc gaccgcaaac atttaaaaaa ttgccactgc caaatctaaa atgccgtctg
     8641 aaagactttt cagacggcat tttgcggagt ctttaaaaca gagaatccaa ctgctgctgt
     8701 ttggaaccag tattactcgg aagcaccggc gtttcctgca tatcttggcg gacttcgtca
     8761 tccgccgcct gccgtccgcc ttctgccgcg cccccgtcat cttcttttgc ccgtcgggaa
     8821 ggttgcggcg caataccgct gttgtccagc gtcaagcccg gatcggttac catacgttcc
     8881 ttcatatagt attcgccatt gctgctgacc acaccttcag gcattttcat ccccttgccc
     8941 tgctttcctt tcaacgcaaa acgcatatag tccacccaaa ccggcaccgc aatcgtaccg
     9001 ccgtagccga cacgccccat actcttaggt ttgtcgaagc cgatatatac ggcagtaacc
     9061 acatcagggt taaaaccgac aaaccacgca tccttattgt cattggtcgt acccgtttta
     9121 ccggcaatat ccgttcttcc caacgcagct gccccccttg ccgtaccgac acggaccaca
     9181 tcctgcataa tcttatacat aatataggca ttgcgcggat cgattgcctg aggcgcattt
     9241 tgcccggcct ccaaaggttg catttgggcg cgcaacctgc cgtctctgtc ataaatctta
     9301 tcgattacgt gcgaagaaac cctatatccg ccgttcgcaa atacgctata tgcctccgcc
     9361 actttcaacg gcgttgtctc gcccgtacct aaagccatag acaggcttgc cggcagctcg
     9421 gacgacctga agccgaaacg ccggatatac tgttgcgcgt aaccgacacc gatagacatc
     9481 aaaatacgga tggaaaccat attcttggaa gccgtcagag cctgtctcaa agtaatgtag
     9541 ccggaatatc tgccgtctga atttttaggt gtccaaaccg aaccgttcgg ccctttcccc
     9601 ggcagggaaa tcggcgcatc gttaaccact gtggacgcgg tcatcccctt agataatgcc
     9661 gccgaataga caaacggctt aaaggtcgaa cccggctgcc gcattgcctg aacggcacga
     9721 ttgaatgttt tgctgtgaaa atcataaccg ccgaccagcg cgcgcacagc tccggttttt
     9781 gcatccagcg aaaccaaagc cccctgcagc aacggctctt gaaccaccgc ccaacgcccg
     9841 ccgttgtttt tgacacggat gaccgcgccc ctgcggatac ggtcctcccc cattttttca
     9901 ttattgaccg cgcgggccgc aaaacccaag gcgcgcctgt caagcgtaac ccgcctgccg
     9961 ccgggcagct gtatgacgac atttttcttt ttagtcacat ccaacacaac ggcgggaacc
    10021 attttatcga cggtatagag tcccgacaga tactggctga cagtctcctc gacatcttca
    10081 ctcttactca aatcgatata gttttccgca ccgcggtagc tgctgccgcg atcgaaattc
    10141 cgtagagcct tgcgcaatgc ctcggttgcc accttctgat gatcagcgcg gaccgtggta
    10201 taaaccttaa aaccctgcgt ataggcatct tcaccgtatt tctcatacag ttcctgacgc
    10261 accatttccg ccacatataa agcactctga tcgattttct gaacaaaccg ctcgtaatgc
    10321 agttcctcat tcaacgcctg atcgcgctgt tgcacggtaa tcatcttctc ctcgagcata
    10381 ttgttcaaaa tatacttctg gcgcaacttg gcacgttccg gattaacaat cggattatag
    10441 gcagacggag ccttgggcag tcccgcaagc atggcggctt ccgccaaagt caaatctcgg
    10501 acattcttat tgaaatagat ttgcgcggca gatgcaaaac cataggcgcg ctgaccgagg
    10561 taaatctgat tgaaatacaa ctcgaggatt ttgtctttgc ttaaagactg ctcgatttta
    10621 taggcaagca acacctcatt gaatttgcgt gtgaacgttt tttcactgct caaataaaaa
    10681 tttttcgcca cctgctgcgt aatcgtactc gcacccgact gcacgctgcc ggacacgaca
    10741 ttgccgacgg cagcgcgggc aacaccccaa acatccaccc cccaatgccg gtaaaagcgt
    10801 ttatcctcgg cggcgataac cgcattccgc aacacctctg ggaaatcgcc gatttttgta
    10861 aattcgcgcc gctgctcccc atacataccg atgacttccc catccgccga ataaatagtc
    10921 aacggcattt taggctggta atgctgcaaa gaatccaaag acggcagttt cggatacgtt
    10981 accaaaatag caatggcaac caaacccact ccaaatacac aaaacccaaa aaccaaacca
    11041 aaacaagtcg ttaaaatctt tttaatcata gctgaataat aatttaccat tattggtatt
    11101 aaataaagta aaatagcaac cgatttctac aaagcacggt ttcaatgtgc aaagaacaag
    11161 gaatccatta cggataccga aacggttact cactgtacaa ataaagcagg aactttcatc
    11221 atgcgcttgt ttaaaagctt gaaaaaccct aaaaaaacag atgccaagct ccctaaaaaa
    11281 tcttcgggac tcaataaccg cgcggcaatc ggcatcgata tcgaccagca ttccatcaaa
    11341 atggtccaat tgtcaggacg tagtttaaac caaattcaat tggaaaaata cgtcattgcc
    11401 aaattaccaa agaatatcat tcaaggcaat aaagtccaaa attacgatca acttgttaca
    11461 tatttgcaac aagcctatgc caaactgggt acgtcgtgca aaaacatcat cgcgtccgtc
    11521 ccgcaaaatc tggcaaccat cgaacaattg acctacacag acaaagatgc agaattagac
    11581 ctgcaggggt tcgtggagtc ctccatctcc gaagtcagct cgatatcgct cgaagaagcc
    11641 aattacgact atcaggtctt gtcccaatcg gccgccggcg aagctgtgtt ggccgtcgca
    11701 tcgagaaagg atgaaatcga acccctgatt gacgcattca acgccgccgg tatgaaatta
    11761 tccgcgcttg atgtggacat tttcggacaa tacaacgcct acgcgctatg gataaaccat
    11821 ttcgcccccg agcttgcagc cgaaaaagtc gccattttcg gcgtatatgc cgcacagacc
    11881 tacgccttgg tcatccaaga cggaaaaatc ctatacaaac aggaaacctc cgtcagcgaa
    11941 gaacagctca accaactcat ccagcgcacc tatcaggtaa cagaagaaaa agcggaagaa
    12001 atcatcaact ccccgcaaaa accttccgat taccaagaaa gcgtggcaaa ctatttcaac
    12061 cagcagatta cccaagaaat acaaagggtc ttgcagtttt attacaccac gcagaccgca
    12121 gacgatatga ccgacatcaa gcatatcctg ctgaccgggg aagcggcgcg ccaggaaggc
    12181 atcacccaaa ccgtcgcctc acaaaccaat gcagatgtac aatgcgtcca tcccgcgcgt
    12241 tattttgcgg acaacctcaa aacagacaaa caacaattcg aacttgatgc gccgacactg
    12301 accagggcgt tcggtttggc ggtacgggga ttataattat gaacaattta atcaaaatca
    12361 acctcctccc ctacagggaa gagatgaaca agcgcaaaca gcagcagttt aaaacgctga
    12421 tgtacggtgc cgtgctgacg ggcgttgccg ccgttgcggc aacctacctg tttatcgaca
    12481 atatgatcaa taaccagtcg gaaagaaaca cgctgctgga aacctccatc gcacacttgg
    12541 ataccgagct gtcggaaata caaaagctca aacaggaaaa agatgccttc ctgattaaga
    12601 aaaacaaaat cgaggagctc cagctcaaac gcctccaagc cgcaaaaatc ctcgacagcc
    12661 tgaatgaggc cgtccccgga agcacctacc tgacctcgct ggatgccgtt accgccgact
    12721 cttatcggct cagcggcagg acatccagcg acaaccgcgt tgccgccatg atgagggcga
    12781 tgcccaatac cggcatattc aagcaacccg aattgttaag catcaagaaa aacaattcgc
    12841 atcaagaatt tacccttcag gcaacattac aacccatcgt aaaggcggcc gaatccaaag
    12901 agaatccggc ttcgggaaac gcacaggagg caaactgaat ggcttctaaa tcatctaaaa
    12961 ccaacttgga tctcaacaac cttcacctgc tcaaccttcc tgccaggctt tttatcgccc
    13021 tgctggccgt tgccgccgtg ctggggctcg gttatgccgg attgttcaaa agccagatgg
    13081 aatcccttga ggaatacgaa gcaaaagaaa ccgaactgaa aaacacctac aaacagaaaa
    13141 gtatcgacgc tgccagcctg aacaacctga gggacgaact tgcctcaatc cgctctgcct
    13201 tcgatatcat gttgaaacag ctgccgacag atgcagaaat tcccaatctg gttcaagagc
    13261 ttcatcaggc aggttcgagc aacggcctgc gcttggacag cgttatgccc caacctcccg
    13321 tagatgacgg ccccatcaaa agattaccct attccatttc cattaccgga aattacgaac
    13381 agatcagcca atttacccgc gatgtcggca gcctctcccg aatcattacc cttgagtcgc
    13441 tgaaaatcgc ccaatctccg gaaaacggcg gcaatcctga cggcaagagc agcatcctga
    13501 acctcagcgc cattgccacc acctaccaag caaaatccgt agaagagctt gccgcagaag
    13561 cggcacaaaa tgccgagcaa aaataactta cgttagggaa accatgaaac actatgcctt
    13621 actcatcagc tttttggctc tctccgcgtg ttcccaaggt tctgaggacc taaacgaatg
    13681 gatggcacaa acgcgacgcg aagccaaagc agaaatcata cctttccaag cacctaccct
    13741 gccggttgcg ccggtataca gcccgccgca gcttacaggg ccgaacgcat tcgacttccg
    13801 ccgcatggaa accgacaaaa aaggggaaaa tgcccccgac accaagcgta ttaaagaaac
    13861 gctggaaaaa ttcagtttgg aaaatatgcg ttatgtcggc attttgaagt ctggacagaa
    13921 agtctccggc ttcatcgagg ctgaaggtta tgtctacact gtcggtgtcg gcaactattt
    13981 gggacaaaac tacggtagaa tcgaaagcat taccgacaac agcatcgtcc tgaacgagct
    14041 aatagaagac agcacgggca actgggtttc ccgtaaagca gaactgctgt tgaattcttc
    14101 cgacaaaaac accgaacaag cggcagcacc tgccgcagaa caaaattaag aagaggatta
    14161 ctccattatg aataccaaac tgacaaaaat catttccggt ctctttgtcg caaccgccgc
    14221 ctttcagaca gcatcggcag gaaacattac agacatcaaa gtttcctccc tgcccaacaa
    14281 acagaaaatc gtcaaagtca gctttgacaa agagattgtc aacccgaccg gcttcgtaac
    14341 ctcctcaccg gcccgcatcg ccttggactt tgaacaaacc ggcatttcca tggatcaaca
    14401 ggtactcgaa tatgccgatc ccctgttgag caaaatcagt gccgcacaaa acagcagccg
    14461 tgcacgtctg gttctgaatc tgaacaaacc gggccaatac aataccgaag tacgcgggaa
    14521 caaagtttgg atattcatta acgaatcgga cgataccgtg tccgcccccg cacgccccgc
    14581 cgtaaaagcc gcgcctgccg caccggcaaa acaacaggct gccgcaccgt ctaccaagtc
    14641 cgcagtatcc gtatccaaac cctttacccc ggcaaaacaa caggctgccg caccgtttac
    14701 cgagtccgta gtatccgtat ccgcaccgtt cagcccggca aaacaacagg cggcggcatc
    14761 agcaaaacaa cagacggcag caccagcaaa acaacaggcg gcagcaccag caaaacaaac
    14821 caatatcgat ttccgcaaag acggcaaaaa tgccggcatt atcgaattgg ctgcattggg
    14881 ctttgccggg cagcccgaca tcagccaaca gcacgaccac atcatcgtta cgctgaaaaa
    14941 ccataccctg ccgaccacgc tccaacgcag tttggatgtg gcagacttta aaacaccggt
    15001 tcaaaaggtt acgctgaaac gcctcaataa cgacacccag ctgattatca caacagccgg
    15061 caactgggaa ctcgtcaaca aatccgccgc gcccggatac tttactttcc aagtcctgcc
    15121 gaaaaaacaa aacctcgagt caggcggcgt gaacaatgcg cccaaaacct tcacaggccg
    15181 gaaaatctcc cttgacttcc aagatgtcga aatccgcacc atcctgcaga ttttggcaaa
    15241 agaatccggg atgaacattg ttgccagcga ctccgtcaac ggcaaaatga ccctctccct
    15301 caaagacgta ccttgggatc aggctttgga tttggttatg caggcacgca acctcgatat
    15361 gcgccagcaa gggaacatcg tcaacatcgc gccccgcgac gagctgcttg ccaaagacaa
    15421 agccttctta caggcggaaa aagacattgc cgatctgggc gcgctgtatt cacaaaactt
    15481 ccaattgaaa tacaaaaatg tggaagaatt ccgcagcatc ctgcgtttgg acaatgccga
    15541 cacgaccgga aaccgcaata cgcttgtcag cggcaggggc agcgtgctga tcgatcccgc
    15601 caccaatacc ctgattgtta ccgatacccg cagcgtcatc gaaaaattcc gcaaactgat
    15661 tgacgaattg gacgtacccg cgcaacaagt gatgattgag gcgcgtatcg tcgaagcggc
    15721 agacggcttc tcgcgcgatt tgggcgttaa attcggcgcg acaggcaaga aaaagctgaa
    15781 aaatgataca agcgcattcg gctggggggt aaactccggc ttcggcggcg acgataaatg
    15841 gggggccgaa accaaaatca acctgccgat taccgctgcc gcaaacagca tttcgctggt
    15901 gcgcgcgatt tcctccggtg ccttgaattt ggaattgtcc gcatccgaat cgctttcaaa
    15961 aaccaaaacg cttgccaatc cgcgcgtgct gacccaaaac cgcaaagagg ccaaaatcga
    16021 atccggttac gaaattcctt tcaccgtaac ctcaatcgcg aacggcggca gcagcacgaa
    16081 cacggaactc aaaaaagccg tcttggggct gaccgttacg ccgaacatca cgcccgacgg
    16141 ccaaatcatt atgaccgtca aaatcaacaa ggactcgcct gcgcaatgtg cctccggtaa
    16201 tcagacgatc ctgtgtattt cgaccaaaaa cctgaatacg caggctatgg ttgaaaacgg
    16261 cggcacattg attgtcggcg gtatttatga agaagacaac ggcaatacgc tgaccaaagt
    16321 ccccctgttg ggcgacatcc ccgttatcgg caacctcttt aaaacacgcg ggaaaaaaac
    16381 cgaccgccgc gaactgctga ttttcattac cccgaggatt atgggtacgg ccggcaacag
    16441 cctgcgctat tgatgcgtca aaataagggc atatgtttta cggcatatgc cctttcttta
    16501 tgcttttgcc gcgaccgaaa tgccgtcatt cccgcgcagg cgggaatcca gtccgttcag
    16561 tttcggtcat ttctgataaa ttcctgttgc ttttcatttc tggattccca cttttgtggg
    16621 aatgacggcg gaaggg
//
LOCUS       DAWKST010000029        16630 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.029, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000029 DAWKST010000000
VERSION     DAWKST010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16630
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.029"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(3..227)
                     /locus_tag="WJL87_000659"
     CDS             complement(3..227)
                     /locus_tag="WJL87_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ7799124.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQEGVFTLVVKHK"
     gene            336..947
                     /locus_tag="WJL87_000660"
     CDS             336..947
                     /locus_tag="WJL87_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEZ7799125.1"
                     /translation="MRRTALLLLALTAGTSLAAGFNQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGDVPADTKGLVQRLKERAGRYAPSIKPHNK"
     gene            complement(1007..1879)
                     /locus_tag="WJL87_000661"
     CDS             complement(1007..1879)
                     /locus_tag="WJL87_000661"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ7799126.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPTDPLKFKDGKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRREMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(1916..2701)
                     /locus_tag="WJL87_000662"
     CDS             complement(1916..2701)
                     /locus_tag="WJL87_000662"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ7799127.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIRTTLALMHGMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAMAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAENA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
     gene            complement(3128..3220)
                     /locus_tag="WJL87_000663"
     tRNA            complement(3128..3220)
                     /locus_tag="WJL87_000663"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3184..3186),aa:Ser,seq:gct)
     gene            complement(3377..3574)
                     /locus_tag="WJL87_000664"
     CDS             complement(3377..3574)
                     /locus_tag="WJL87_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799128.1"
                     /translation="MAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDVAL
                     ARFPEDAWFQEIKQDAQKHFA"
     gene            complement(3679..3996)
                     /locus_tag="WJL87_000665"
                     /pseudo
     CDS             complement(3679..3996)
                     /locus_tag="WJL87_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            complement(4019..4780)
                     /gene="kdsB"
                     /locus_tag="WJL87_000666"
     CDS             complement(4019..4780)
                     /gene="kdsB"
                     /locus_tag="WJL87_000666"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ7799129.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAV
                     RVVVATDHPDIQTACQAHGVEVVMTSNRHESGTTRLAEASAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPFETAVLRHIGIYAYRVGFLQRYTEMSVSSLETIESLEQLRVL
                     WHGYSIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            complement(4777..4959)
                     /locus_tag="WJL87_000667"
     CDS             complement(4777..4959)
                     /locus_tag="WJL87_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ7799130.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARALSEEELKA"
     gene            complement(5028..5609)
                     /locus_tag="WJL87_000668"
     CDS             complement(5028..5609)
                     /locus_tag="WJL87_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ7799131.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAKAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            complement(5815..6849)
                     /locus_tag="WJL87_000669"
     CDS             complement(5815..6849)
                     /locus_tag="WJL87_000669"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ7799132.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAESRAEDAGDEPLLLFRKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPSADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            complement(6889..7160)
                     /locus_tag="WJL87_000670"
                     /pseudo
     CDS             complement(6889..7160)
                     /locus_tag="WJL87_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7396..8676)
                     /locus_tag="WJL87_000671"
     CDS             complement(7396..8676)
                     /locus_tag="WJL87_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ7799133.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYKTREDKDRMDESKQFYAIEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLSEWKL"
     gene            complement(8898..9518)
                     /locus_tag="WJL87_000672"
     CDS             complement(8898..9518)
                     /locus_tag="WJL87_000672"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ7799134.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGETLREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQTREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAVIDSNLGLDEVRNSIEKVLDGHFGC"
     gene            complement(9578..10573)
                     /gene="mltG"
                     /locus_tag="WJL87_000673"
     CDS             complement(9578..10573)
                     /gene="mltG"
                     /locus_tag="WJL87_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ7799135.1"
                     /translation="MLKKMLKWTAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYALGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKIIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLQIYQTAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLVEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLIEHNAAVRKYILKK"
     gene            10657..11229
                     /gene="ampD"
                     /locus_tag="WJL87_000674"
     CDS             10657..11229
                     /gene="ampD"
                     /locus_tag="WJL87_000674"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ7799136.1"
                     /translation="MDNHAEAHWQDGWLQSVRHIPSPNFGPRESKEEVSLVVLHNISL
                     PPFEYGTDAVEKLFANRLDPNDHPFFGLIHTLRVSSHFLIKRDGKTVQFVSCGDMAYH
                     AGVSSFRGREKCNAFSIGIELEGCDFEPFAEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            11423..12709
                     /locus_tag="WJL87_000675"
     CDS             11423..12709
                     /locus_tag="WJL87_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ7799137.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGSVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKVSPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPAPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            12857..15382
                     /gene="ligA"
                     /locus_tag="WJL87_000676"
     CDS             12857..15382
                     /gene="ligA"
                     /locus_tag="WJL87_000676"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ7799138.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGKPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVANIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQGGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEQMQQKRPELPYEIDGMVVKVNSLAQQRELGFI
                     SRAPRWAIAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMRETAVAVSDGIGHRQDDLFAET
                     PSANQTQSVPLHKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETADKTVAESDQMPSEG
                     SSVGASGKHKKQPVKWAENILAGIEASKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVARSIAHFFAQAEQQAMIDELLAAGVAPQTQAVTIPP
                     ARHAEPQRWIARLPGFKISENKAQALWELAGKNIEGLQTDKALPTDWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            15439..16614
                     /locus_tag="WJL87_000677"
     CDS             15439..16614
                     /locus_tag="WJL87_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ7799139.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGALEGAGFVHYETSAFAKPAMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPSEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLNDLLQCFL"
BASE COUNT         4004 a         4895 c         4056 g         3675 t
ORIGIN      
        1 atttatttgt gcttgacgac cagcgtgaac acgccctcct gttcggaagc atccaacagc
       61 acatgaccgg tttggcggca aaaagcctca aaatcccccg gtgcgccgcc gtcggttgcc
      121 agaacggtca acacgtcgcc ctgctgcatt tgcgccaaag cctttttcgc ccgcaaaatc
      181 ggcaggggac atttcaatcc ggttacgtct aaagtttcgc tgttcatcgt ttgtaccgtt
      241 taaaaaatgg gaatcggggg gagattatac cccagttgct tgcagccccc tttgccaacc
      301 cataaaataa cgccgttttc aatttggaga aacttatgcg ccgcaccgcc ctcctgctgc
      361 tcgcactcac cgccggcacg tcccttgctg ccggcttcaa ccaaaaagac acgccgatca
      421 acacccgcta ccgcgaaacg cccgaagaag ccgccgcccg cgaatttaaa gaacataccg
      481 ccaaactgcc gccgctgccc gatacgcact cggacggctg gttcgacatt tatgtggatg
      541 aaaattacgg caaacagccg aaaatcctgc tcgacagcct gcaaattatg cccgcgcccg
      601 acggcagcat ccgctacatt ctcaatatcc gctccgacaa aggctacgac aacctgactg
      661 ccgaaggcat attctgcgcc cgcttctcca tcaggttcgg caacggcaaa ccttcctcct
      721 ataaagtgtt cggctacggc gacacggtca accgccgctg gatacagccc cgaaacgccg
      781 aatggaaacc gataggcggt acattaagcc gcaacgacgc actgcgcacc gtcctgtatc
      841 aggcattctg cgagggcgat gtacccgccg acacgaaagg tttggtacaa cgcctgaaag
      901 aacgtgccgg acgctatgcc ccgtcgataa agccgcacaa caaataacca gacacaaaaa
      961 taccgtctga aacatcggtt tcagacggta ttcgattcat cagccatcaa gcggcggaaa
     1021 ctttgtcctg acggcacaac agcgtaatca aatcactgat gcgccgcttc atctcacgcc
     1081 ggtcaacaat ctggtcgatt gcgcctttct ccagtaaaaa ctcagcacgc tggaagcctt
     1141 ccggcaaggt ttcccgtaca gtctgttcaa taacgcgcgg tcccgcaaaa ccgattaggg
     1201 cattgggttc agccaatacg acatcaccca aaaatgcgaa gctggcggat acgccgccca
     1261 tagtcggatc ggtcaacacc gatataaacg gcaggcgttt ttccgtcagc aaatgcagcg
     1321 cggcactggt tttcgtcatc tgcatcaatg agttcacacc ctcctgcata cgcgcaccgc
     1381 cggaagccgc cacacagacg aacggacaat tgtcggcaac cgcccggcgg acgccctgta
     1441 cgaatcgttc gcccacaacc gaacccatcg aaccgccgat aaagcggaac tcaaacgcag
     1501 caaccacaac gggcagaccg ttcatcttgc ctttcattac caccaaagca tcatcttccc
     1561 cggtcagctt gcgtgccgca ctcaaacgat ccggatattt tttgccgtct ttaaacttca
     1621 aaggatctgt cggtttgaca tttccggcaa cctcctctct gccatcctca tccaaaagca
     1681 gattcaggcg ttgtcgtgcc gacaacggat tgtggtggtt gcatttcgga caaacctgat
     1741 tgttctgctg caactcggtc gaataaacgg ttgccgaaca agacggacat ttgtgccaca
     1801 aaccctcggg aacattggaa gaaccgtctt tgctgcggtt tttgattttg ggtggcagga
     1861 ttttatctaa ccagctcatg gacaactcct aaaatattta gaatgcgtga cagaatcagc
     1921 gcacggcatc cttcaactct ttgaccaaag caccgacggc cgcagcctcg ttgcccgcat
     1981 tgttttcaat ctctttcaca atccggctgc cgacaatgac cgcgtcggca acccggccga
     2041 ttttgcgcgc gttttccgca ttgctgatgc cgaaaccgac accgatggga atatcgatat
     2101 actgatgcaa atactctatt ttacgcgaaa cctcatccgt atccaaactt gccgcgcccg
     2161 ttacgccctt gagcgaaaca taatagacaa atccgcctgc cagctcggca atggttttaa
     2221 tacggtcttc cgtcgtcgtc ggcgcaatca ggaaaataca gtcgaccccg ttatccttca
     2281 gctcgcgata gagcggatcg atggtttcga caggggaatc caccgtcaac acgccgtcca
     2341 cacccgccat tgcggcttcc tgagcaaact cccgataacc catcttatgt acagggttca
     2401 aatatcccat caaaacaacc ggcgtttgcg tgtcggtttc acggaatttt ctgacgacat
     2461 ccaagacatc gcgcagcgaa atcccgtttg ccaacgcccg ctccgccgca cgctgaataa
     2521 ccggtccatc cgccatcgga tcggaaaacg gcacgcccaa ctccaaaata tccgcaccgt
     2581 ttgcaaccat gccgtgcatc aatgccaaag ttgtccgaat atcggggtcg cccaccgtaa
     2641 tatagggaat caatgccttt ccgccatcca aagcggcaaa agcctgccgg attctgctca
     2701 tttgtctctt ccatcctatc ggcaaatatg ccataaaaca caatcagtaa atttttccca
     2761 gtataacatt tgcggtacgg ataggcgaat acggcaaaca gaaaccacgc aaatcagagc
     2821 cgccgtttga acgaggcgga aataaacggc aagcatctcg gattatagtg gattaacaaa
     2881 aatcaggaca aggcggcgaa gccgcagaca gtacaaatag tacggagccg attcacttgg
     2941 tgctccagca ccttagagaa tcgttctctt tgagctaagg cgaggcaacg ccgtactggt
     3001 ttttgttaat ccactataac aaaattcaga atcaagacag ggcaatacca tactggtttt
     3061 gcaccaaccc gctataaaat tgcataaaaa aaatagacgc tgtaaaacac agcgtctatt
     3121 ttttatctgg cggagtaaga gggattcgaa ccctcgatgc aggttgaccc cacatgcttc
     3181 cttagcaggg aagtgccttc agcctctcag ccattactcc taaggaggct gtaactataa
     3241 tcaacttttc cgataccgtc aaggaaaaac agaaagcaaa tattaatctt ttgaaataaa
     3301 ataagattta ccctgattat acccaatcag gcacttcttt tcttcctgaa tggaaatatt
     3361 cctgactgcc acatcctcaa gcaaaatgct tttgtgcatc ctgtttgatt tcctgaaacc
     3421 acgcgtcttc aggaaaacgt gcaagcgcga catccaatgt tgacagcagg ttttgcgaca
     3481 gggctttggt caggtattcc tcctcgttgg cttctgccgc tttgtcgggc tgttgtgcca
     3541 aaccgtcggc acgggctttg tgtccgtgtg ccaaatatag tggattaaca aaaatcagga
     3601 caaggcgacg aagccgcaga cagtacagat agtacgacaa ggcgaggcaa cgccgtactg
     3661 gtttaaattt aatccactat aattgagcgc ggtaattttt tgcgcccaaa gctgcggtat
     3721 gtcgggattg tcttcaatca tgacggaaag gatggatacg gcggtcgcca gcctgccgcc
     3781 gttcattcta aggtcgaggg aacggttggg cggcagggtg ctgagtgttt ctgccattct
     3841 gaaccaaaat tcgccgctgg cttcgtcggg tgcgttcaat ttaagcattt catagtgaac
     3901 ctcctgatca tcgttttgca cgagacccca tacttcggca aggattttct gttcttcacg
     3961 gcttaagtgc tgccactttt caatatgttg ctgcatatct tttccctttg aacctgtttt
     4021 atacggtctg aaatacggcg cggaccctgt ccaaatcttc ctgcgtatcc acaccggcgg
     4081 cgggggcttc tttggcggtt tcgacggcaa tcgagtagcc gtgccacagg acgcgcagtt
     4141 gctccagcga ttcaatggtt tccagcgacg aaacgctcat ttcggtgtag cgttgcagga
     4201 agccgacgcg gtaggcgtag atgccgatat ggcgcaggac ggcggtttca aacggcattt
     4261 cgcgttttcc tgcacgcatc gcatcgcgcg gatagggaat cggggcgcgg ctgaagtaga
     4321 tggcgttgcg gtttttgtcg aggacgactt tgacggcatt gggattcatc aattcgtcga
     4381 aatcgtgcaa ttcgtgtgcc gcagttgcca tctgtacgtt gttttccacc aagacttcgg
     4441 cagtgcggtc gatgagttcg ggggcaatca gcggctcgtc gccctgtacg ttgacaacca
     4501 ccagatgcgg cggcagcttc agcgcggcag aggcttcggc aaggcgcgtc gtgccgcttt
     4561 cgtgccggtt tgaagtcatg acgacttcga cgccgtgcgc ctgacaggcc gtctgaatat
     4621 cgggatggtc ggtggcaacg acgacgcgca cggctttact ttttgccgcc tgttcggcaa
     4681 cgcgcacgac catcggttta ccgtggatat ccgccaaggc ttttccgggc aggcgcgatg
     4741 aatccagccg cgccggaatc aatacgacga attcagtcat gctttgagtt cctcttcgct
     4801 caacgctcgc gcttcgtttt ccagcatata gggaatgccg tctttaatcg gataggcaag
     4861 cttcgcctga cggctccaca attcctgttt gtcctgatga tattccagcc tgcctttggt
     4921 aacggggcag acgaggatgt ctaagaattt tttttccata gggttgcttt cgggtttgat
     4981 ggttcaatca atcggatgcc ctcttcaata tttcagacgg ctatcattta tttctgatgc
     5041 cttttcccaa cctgtccggc ttgtttacag cccgcatcgg gatttttacc gccgcagatg
     5101 atgcgccgca actcttccgt aaacgcgggc agatgatgtc gcgcgatttt ccctgacaat
     5161 gcagtccaag atactgctat ttcactcatc gatttcttga ttaagcgcgg atttttggca
     5221 acgacggact gaccgacagg cgaaccgtaa aaggcaatca tgccgtcaat ttcttcttgc
     5281 gtgtatatct cacgggcatt cttcaataaa gtattgcgga cagcctgttt cacttcgggc
     5341 gtaatcaaat ctttcaaaac attctcacga tagcggttaa aggcttttgc cgcctgatct
     5401 tttttcgctt ccggcatttc ggcaagggct ttgtccgcat acggtttaaa tccggcatta
     5461 aagccctcaa tcatattttt ttctatatcc cggtcaaaat tctgcgtatc cagccaacgc
     5521 gccaacgacg cgtcgctggg cggggcggca aaagcgttgg tgcacaatgc cagcgtggcg
     5581 aagggcaata acaaggtttt cagtttaata ttcaatactt taacgaatga ttaatgcaga
     5641 taaaaaatac gaccgatttt ctcattcctg tccaagtgga aatccggaga tttgggattt
     5701 ctgaagcctc ctaggcattc ctgaaaattc gagacttaga ttcccgcctg cgcgggaatg
     5761 acggcgcgag gcgttcttat tcaaatccgt aatcactccg cccaaaccgt gctttcagac
     5821 ggccttcggt acatcttcca accgctccaa cacaaacgcc gccaaatcag gttcgattat
     5881 cgcacaaacg ggcaacaccc aaacattatc ggtgcaaatg ccgtctgaaa atttgaccgc
     5941 atctttctcc gtaataatga ccgcgtccgc atcgggcaaa tctgccgccg aaatgtcggc
     6001 gtggtcaggc agcgcgacgg ttcgcttcac ggtaatgccc atattccgca acgaatcgaa
     6061 aaaacgcgcc ggcttggcaa tacctgccac ggcgacgacg gtttgatttt tcagacggcc
     6121 tgtgtccagt atttcggacg gattgttcaa acggtaaatc cgtcccgctt cgatacggct
     6181 gtgaaacata ttttcagacg gcctaaacag cgcatccgcc ttgccgccgc tgacgacgac
     6241 cgcatccacc gaatccagcc gcaacaaagg ttcgcgcaaa ctgccgttgg gcagtaaatc
     6301 caaatccgtg cgccccgtat ccgccgacgg aaacaccgcg atttccacat ctcgccgcag
     6361 ggcgtaatgc tgcaaaccgt cgtccgccac aatcagtccg atgtcgggat gcgccgccag
     6421 caacgccctg cctgcctctg cacggctgct gcccaccgcc gtcggcgcac cggttttgcg
     6481 gaacagcagc aaaggctcat cgcccgcatc ttccgctcgg ctctcagcat tcaatacatg
     6541 aaccgccttg ctcttgcgcc cgtagccccg gctgatgatg ccgaccttga cgcccttttc
     6601 ctgcaaaccc gacaccagcg cggcaacaat cggcgttttc cccgtcccac ccgcgtgaat
     6661 attgccgacc acgaccacag gcacgggcag cttttcgctt tgccgttttc ccgataaaaa
     6721 atccgtccgc cgttttgccg caattttggc aaacagcccg gagagcggct taagcagaaa
     6781 agacaaaacc ggataaggtt tttgccaatg ccgttcgata acggtatgaa atttgaaaca
     6841 attcggcata ttcgatacaa atcctcgaag caaaatcagc ccggggcatt aatcatgcgt
     6901 acccgataca gacccaccta taaacaacgc gaagaccaaa ccgataagcg aaccaatcag
     6961 gtaaataagc aactgcgctt tacagaaatt ggcgcggttc gggttgcctc tgccgaacac
     7021 ccaaacaaaa attgaccaaa ggaatcatga aaacaatcat cgtcaacacc cactgccctg
     7081 tcgtcatgac gggaggcggt ttgttttgaa gtctcgggca tatcgccaat atgataacgg
     7141 acaggtttca tctctttcat ttgccagtat ctttcattta tagtggatta acaaaaacca
     7201 gtacggcgtt ggctcgcctt agctcaaaga gaacgattct ctaaggtgct gaaacaccaa
     7261 gtgaatcggt tccgtactat ttgtactgtc tgcggcttcg tcgctttgtc ctgatttttg
     7321 ttaatccact atatcaatct gacagcaaaa tgccgcctga aaacagttca gacggcattt
     7381 taaaccgcaa acggcttaca gcttccattc gctcagcttg gcagcgtaag cttccaaatc
     7441 tgcaatcgga cgggttgcca cgccgctttc catcgctgct ttggcggcag ccgtagcaac
     7501 gcgcggcagc aggcgggaat cgaacggagt cggaatcagg tattccgcgc cgaattcaaa
     7561 tttcttaccg taagcggcaa ccacttcttc ggttacttct tccatcgcca aatccgccaa
     7621 agcatacacg caggcgcgtt tcatttcttc gttgatggtc gtcgcgccga catccaacgc
     7681 gccacggaag atgaacggga agcacaatac attgttcact tggttcggga agtcggagcg
     7741 gccggtaccg ataaccacgt ccggacgggt ttctttcgcc agcggcggca ggatttccgg
     7801 attcgggttg gccatagcga acacgatggg tttttcgttc atcgtgttca acatttcggg
     7861 ggtcagcagg ttcgcgccgg acagacccaa gaagatgtct ttgcctttaa ccgcatcggc
     7921 caatacgcgc tggccgttgt cttcaatggc gtagaactgt ttagactcat ccatgcgatc
     7981 tttgtcttcg cgggttttgt aaatcacgcc ttttgagtcg caaacggtca cgttttcgcg
     8041 tttcaagccc aaatccagca attggttcaa gcaggcaatc gcggccgcac ccgcaccaga
     8101 gcaaaccaag gtcgcttctt cgattttacg gccggtaaaa cgcagggcgt tcaatacggc
     8161 agcggcggta atgatggccg tgccgtgctg gtcgtcgtgg aatacgggga ttttgcagcg
     8221 tttgcgtaat tcgcgttcga tgtagaaaca ctcgggtgct ttgatgtctt cgaggttgat
     8281 gccgccgaag gtcggctcta aagcggcgat gatgtccacg agtttttgcg ggtctttttc
     8341 atcgatttcg atgtcgaaca cgtccacacc ggcgaatttt ttgaacaata cgcctttacc
     8401 ttccatcacg ggtttgcccg ccagcgcgcc gatgtcgccc aagcccaaaa cggccgtacc
     8461 gttggaaatg acggcgacca agttgccttt ggcggtgtat ttgtaggcat tttgcggatc
     8521 ggcatggatt tccatacaag gagcggctac gcccggagag tacgccaacg ccaaatcttt
     8581 gtcggtcgcc aaagatttgg tcggggtaac ggaaattttg cccggaacgg gtaattcgtg
     8641 gaacttgagg gcggcttctt tcaatgagtt ttccatgtgt caatcctgtt gcgagaaaaa
     8701 gatttgaatc ggactgtccg aaacgctttc gggtcatcat gttacgggcg gttcgttaat
     8761 attgccgacg catcctgcct tgtaaccgag cgtaattcta tcatgccgaa agtttggcgc
     8821 ataccgccat tttcacctgc ggcactgccg tttgattttc ggcgcgggcg gatgcgcttg
     8881 tttcaatatt tgccgcatta acagccgaaa tgtccgtcca acactttttc tatgctgttt
     8941 ctgacttcat ccaagccgag gttactgtcg ataacggcgt aacgttccgg acaggcggcg
     9001 gcacggttca gataaacgct gcgcacacgc atgaagaaat ctgcctgttc ctgctcgaaa
     9061 cggtctttct cgcgcgtctg cccgatacgc gccatcgaca cttccagcgg cacatccaac
     9121 agcagggtca aatcggggcg caaaccgccc tgcacccaat gttccaaaat ttcaatgtct
     9181 tcagacggca tcccccgccc gccgccctga taggcgaagg ttgcatcggt aaaacggtcg
     9241 gacacgacat ggatgccgtc tgaaagcgcg ggcaatatca cgtcttcaat atgctgcatt
     9301 ctggcggcga acatcatcag ggtttccgca cgcaaaccgg ctttggtttc agggttgagc
     9361 aggatttcgc gcaaggtctc accgaccggc gttccgcccg gctcgcgcgt gaacagcacg
     9421 ggcagccccc tccgttcaaa ccatgccttg atgacggcaa ggttggtgga tttgccggca
     9481 ccgtctatgc cgtccaaagt gatgaattgc ggtttcatgc ttgcagttcc ggtaagggta
     9541 tgccgtctga aaacacaaac ttttcagacg gcatggttta ttttttcaaa atatatttgc
     9601 gaacggcggc gttgtgttcg atcaaatcat ggctgaactg gctcaagccc gtgccgtcca
     9661 ttttggacac gaaatacagg tatttttcgc cagacggatg ggcggcggca tcaagcgcgg
     9721 ccttgccggg cagcgcgatc ggggttggcg gcagaccgcc gcgcgtgtag gtgttgtacg
     9781 gcgtgtcgcg gcgcaggtcg gctttacgga ttttgccctt gtatgccgca cccatgccgt
     9841 aaatcacgga cgggtcggtt tgcaggcgca taccgatttt cagccggttg acgaagacgg
     9901 aagcgacatg gtcgcggtcg gcttcatgcc ccgtttcctt ttcgaccagg ctcgccataa
     9961 tcagcatttc ataaggattt ttataaggca gcccgtcctg cctgctttcc catgcctcat
    10021 tcaggcggcg ttgcatcgcc ttgtaggcgg tttggtaaat ctgtaaatcg ctgccgcccg
    10081 catcgatttc gtagctgtcg gggaaaaact gcccttcagg attgccgctg aaggcatcgg
    10141 gcgcaacttc cgccatcagt ttttcattgc tccagccttt ggtgtcgtgt tcgatgtcgg
    10201 gcgttgcgtc gatgattttc ctcatatgcg aaaaacgcga accttcgata atctgcacgg
    10261 taacggaatc cggcctgccg ccgcgcattt tctgcaagat atcccaagcc gacacttccg
    10321 aaggaagcct gtacgtacct gtgtgcaacc tgttatgcac gcccaaagca taggctgccg
    10381 ccgtcaaaac atgtctgctg aacacgatgc ggtcttcggc aagtttcctg ccgaccgacg
    10441 aaataccctg gtttttggca attttgattc ggtatgccct gccgttgtct ttagggacaa
    10501 aaagcagcgc ggcgaaaacg gctgccgata cggtcaaaaa aacggcagtc catttcaaca
    10561 tttttttcaa catggtagga ttcccagcat tcggaatacg tcttaaaatt cgggcgacag
    10621 tataacccaa attgcccgcc ggacaggaag aacatcatgg acaatcacgc cgaagcacac
    10681 tggcaggacg gttggctgca aagcgtgcgc cacattccgt cgccgaattt cggcccgagg
    10741 gagtccaaag aagaggtttc ccttgtcgta ttgcacaata tctcgctgcc gccgttcgaa
    10801 tacggcacgg atgcggtgga aaagcttttt gccaaccgcc tagacccgaa cgaccacccg
    10861 tttttcggat tgatacacac tttgcgcgta tccagccatt tcttaatcaa acgcgacggc
    10921 aaaacggtgc agttcgtatc atgcggcgat atggcgtacc acgcgggcgt atcctcgttt
    10981 cgcggacggg aaaaatgcaa cgcattttcc atcggcatcg aactggaagg ctgcgatttc
    11041 gaaccgtttg ccgaagcgca ataccgttcg ctcgaagcct tgttggacgc aatctgccgc
    11101 cgctacccca tcacagcggt aacggggcac caagacatcg cgcccggccg caaaaccgac
    11161 cccggccatt ttttcgactg gcggcggata cgggggaaag ggtttcccgt ggacagaaat
    11221 gccgtctgaa aaccacagat ttcagacggc atttcggaaa atttccgctt atttcgacac
    11281 actgctgtca gaccctgcaa ttttcgttat aattcaaacg ttataaccgg ccgttccatc
    11341 agactgccgg aaacgcaagc ccgatttaac cgcagtaccc gcacttcgcg catcctgcat
    11401 cccgaaattt aaagtgattg ccatgattta catcgtactg ttcctcgccg ccgtcctcgc
    11461 cgttgtcgcc tacaatatgt atcaggaaaa ccaataccgc aaaaaagtgc gcgaccagtt
    11521 cggacactcc gacaaagatg ccctgctcaa cagcaaaacc agccatgtcc gcgacggcaa
    11581 accgtccggc gggtcagtca tgatgccgaa accccaaccg gcggtcaaaa aaacggcaaa
    11641 accccaagac cccgccatgc gcaacctgca agaacaggat gccgtctaca tcgccaagca
    11701 gaaacaggca aaagtatcac cgttcaaaac cgaaatcgaa accgccttgg aagaaagcgg
    11761 cattatcggc aactccgccc acaccgtttc cgaaccccaa accggacatt ccgcaccgaa
    11821 acctgccgac gcgccggcaa aacctgcacc cgttccgcaa acacctgcaa aaccgctgat
    11881 tacgctcaaa gaactgtcaa aagtcgaatt accctggttt gacgtgcgct tcgacttcat
    11941 ctcctatatc gcgctgaccg aagccaaaga actgcacgca ctgccgcgcc tttccaaccg
    12001 ctgccgctac cagattgtcg gctgcaccat ggacgaccat ttccagattg ccgaacccat
    12061 cccgggcatc cgctatcagg catttatcgt gggtattcag gcagtcagcc gcaacggact
    12121 tgcctcgcag gaagaactct ccgcattcaa ccgccaggtg gacgcattcg cacaaagcat
    12181 gggcggtcag acgctgcaca ccgaccttgc cgcctttatc gaagtggctt ccgcactgga
    12241 cgcattctgc gcgcgcgtcg accagaccat cgccatccat ttggtttccc cgaccagcat
    12301 cagcggcgta gaactgcgtt ccgccgtaac gggcgtgggt ttcgttttgg aagacgacgg
    12361 cgcgttccac tataccgaca cgtcgggctc aaccatgttc tccatctgct cgctcaacaa
    12421 cgagccgttt accaatgccc ttttggacaa ccagtcctac aaaggcttca gtatgctgct
    12481 cgacatcccg cactctccgg caggcgaaaa aaccttcgac gatttgttta tggatttggc
    12541 ggtacgcctg tccggccagt tgaacctgaa tctggtcaac gacaaaatgg aagaagtttc
    12601 gacccaatgg ctcaaagacg tgcgcactta tgtattggct cgtcagtccg agatgctcaa
    12661 agtcggtatc gaaccgggcg gcaaaaccgc attgcgcctg ttctcctaaa atcgaatgca
    12721 atcaaagcgg atatgtcgtt acggcatatc cgcttttttt cgggtaaaat tcagctgttt
    12781 tcaacctatc atttttcaga cgacctttgc ccgtttcatc ccgtatcaga caaaggtcgt
    12841 ctgaaaacct tctctcatga atccaaactc aaaacataac actaacttta ccaatcttct
    12901 taaaccgtct gatagtgata taaaacaatt tgccgcacaa cacatccgcc acctcaccga
    12961 cctgctcaac cgctatgcct acgaatacta caccctcgac gcgcccagcg tacccgatgc
    13021 cgaatacgac aaattattcc gcgagctcga agcgttggaa ttaaaccatc ccgagctcaa
    13081 actgcccgac agtccgaccc agcgcgtcgg cggcgagcct ttagcgggat ttgccgaagt
    13141 gcgccacgaa gtgccgatgc tgtcgctgac caacgctttt tccccgcaag atgaaaacgg
    13201 cgtgttcgac catgccgaaa tgtacgcttt cgaccaacgc gtgcgcgacg gtttggacgg
    13261 cggcaaaccc gaatacgtta tcgaacccaa attcgacggc ctcgccatca gcctgctcta
    13321 ccgcgacggc gtattggtac aggcggcaac gcgcggcgac ggcacaacgg gcgaagacgt
    13381 tacccaaaac atcaaaaccg tcgccaacat ccccctgcgg ctgcacggcg aaaatacgcc
    13441 cgaactcatc gaagtacgcg gcgaagtact aatgcaaaaa gccgattttg ccgccctcaa
    13501 taaaagacaa gccgaaaacg ggcaaaaacc ctttgccaat ccgcgcaacg ccgctgccgg
    13561 cagcctgcgc caactcgatt cgcgcatcac tgcgcaacgc aaactgcact ttttccccta
    13621 ctccgtcgcc cgccagcaag gcggtttcgt cgcggaagaa cacatccaag aactcgccta
    13681 tttccaagca ctcggcttca gtctgccaaa tggcaatttc ggctgtttca aaaatatcga
    13741 cgaagtattg gcgttttacg aacaaatgca gcaaaaacgc cccgaactgc cctacgaaat
    13801 cgacggtatg gtggtcaaag tcaacagctt ggcgcaacag cgcgaactcg gcttcatctc
    13861 acgcgcgcca cgctgggcga ttgcccacaa attccctgcc gaagaagccc tgaccattgt
    13921 cgaagcgatt gacgtgcaaa tcggcaggac gggcgcggta acgccggttg cccgattaca
    13981 acccgtattc gtcggcggcg taaccgttac caacgccacc ctgcacaatc aggacgaagt
    14041 atcgcgtaaa gacgtgcgcg tcggcgatac cgtcgtcgtg cgccgcgccg gagacgtgat
    14101 tcccgaagtt gtgcgcgtga tttttgaacg ccgcccgatg cgggaaaccg ccgttgccgt
    14161 ttcagacggc atcgggcatc ggcaggacga tttgtttgcc gaaacaccgt ccgccaatca
    14221 aacccaatcc gttccgctcc acaagcccta ccgcctgccg acccactgcc ccatctgccg
    14281 cagcgaaatc gaacgcgaag aaggcgaagc cgtcgcccga tgcagcggcg gtatgctttg
    14341 tcaggcacaa cgcgcgcaag gcttaatcca cttcgcctcg cgcaaagcga tggacatcga
    14401 cggcttgggt gaaaaacaaa tcgaacagct tgtcgcgcaa gacctcgtcc gccacttcgc
    14461 cgatctctac cgcctcgaca tcccgacctt gcaaaaaatg aaggaaacag cggacaaaac
    14521 cgttgccgaa tcggatcaaa tgccgtctga aggcagttcg gtcggcgcgt cgggcaaaca
    14581 taaaaaacaa ccagtcaagt gggcggaaaa catcctcgca ggcatagaag ccagcaaaac
    14641 gcccgaactc gcccgcttcc tgttcgcgct cggcatccgc cacgtcggcg aacgcaccgc
    14701 caaaacgctg gcacaggcat tcggcacatt ggaacgcgtc cgccgcgccc ccgaacccgt
    14761 cctcgcctgc ctgcccgaca tcggtacggt agtggcgcgt tccattgccc acttcttcgc
    14821 ccaagccgaa cagcaggcga tgatagacga actgctcgcc gcaggcgttg ccccgcaaac
    14881 ccaagccgtt accatcccgc ccgcccgcca cgccgaaccg caacgctgga tcgcccgcct
    14941 gcccggtttc aaaatcagcg aaaacaaagc ccaagcctta tgggaactcg ccggcaaaaa
    15001 catagaaggg ctgcaaaccg acaaagccct ccccaccgac tggcaggcgt ggcgcagcga
    15061 accacaaaac gccgccctgc tcgaaaacct gaaaaccttc ttcgcgcaaa tgccgtctga
    15121 agacgaggcg gcgcaaggtt cagacggcat caataaagcc gtggcgggca aaaccttcgt
    15181 gttaaccggc accctgccca ccctcaaacg cgaccaagcc caatccctaa tcgaagccgc
    15241 aggcggcaaa gtttccggca gcgtgtccaa aaaaaccgat tacgtcgtcg ccggagaagc
    15301 cgcgggcagc aagctggaaa aagccaatgc cttgggcgtt tccgtcctca gcgaagccga
    15361 actgcttacc ctgctcggct gaacccatgc cgtctgaacc gccttcagac ggcattgccc
    15421 acttttccga accacaccat gcacaccatt tcctttccaa accgcacccg actcaccgcc
    15481 ctgccgcccc tgtcgcttta catccacatc ccgtggtgca tcaaaaaatg cccgtattgc
    15541 gacttcaatt cccacagcct gaaaaacgga ttgcccgaag ccgcctatat cgacgcgctg
    15601 ctgaccgatt tgcagcttga attgcccaat atttggggca ggccggtgga aacgatcttt
    15661 ttcggcggcg gtacgcccag cctgtttcag gcggaatcga ttgaccgttt gttaagcggc
    15721 gtgcgttcgc tgttgcgctt gcagccggaa gcggaaatta ccttggaagc caatccgggg
    15781 acatttgaaa tcgagaagtt tcaaggattt aaagacgcgg gtattacgcg gctttctatc
    15841 ggcgtacaga gtttcaacga cgatatgctc tcaaggctgg ggcgcgtcca caacggtaag
    15901 gaagccttaa ccgctatcga taccgccttg aaattatttg ataaagtcaa tatcgacttg
    15961 atgtatgccc tgccgaacca aaccgttcag acggcattgg acgacgtaca aaccgctatc
    16021 gctacgggcg cgacccacat cagcgcgtat catctgacga tggaaccgaa cacgccgttc
    16081 ggtcatacgc cgccgaaagg tttgccgcaa gacgaagccg ccctcgacat cgaagacgcg
    16141 gtacacggcg cgctggaagg cgcgggtttt gtccactacg aaacatcggc ttttgcgaaa
    16201 ccagccatgc agtgccgcca caatttgaac tactggcagt tcggcgatta tttaggcata
    16261 ggcgcgggcg cgcacggcaa aatttcctat cccgaccgca tcgagcgcac cgtccgccgc
    16321 cgccacccca acgactacct cgccttaatg caaagccaac cgagtgaagc cgtcgaacgc
    16381 aaaaccgtcg ctgccgaaga tttgccgttc gaattcatga tgaacgccct gcgcctgacc
    16441 gacggcgtac ccgccgcgat gttgcaggag cgcacgggcg taccgagtgc caaaatcatg
    16501 gcgcaaatcg aaacggcaag gcaaaaaggc ctgctggaaa ccgaccctgc cgtattccgc
    16561 ccgaccgaaa aaggacgctt gtttttaaac gatttgctgc agtgtttttt atagtggatt
    16621 aacaaaaacc
//
LOCUS       DAWKST010000030        16542 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.030, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000030 DAWKST010000000
VERSION     DAWKST010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16542
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.030"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            118..1293
                     /locus_tag="WJL87_000678"
     CDS             118..1293
                     /locus_tag="WJL87_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ7799140.1"
                     /translation="MLNTFLSPWPCFTQEEADAVSKVLLSNKVNYWTGNECREFEKEF
                     AAFAGTRYAVALANGTLALDVALKAIGIGAGNDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETIKAALTSNTKAIIVVHLAGMPAEMDGIMDLAKEHDLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWEKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRLPEWTACRQAHAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVSELNARKVPCYQGSCSEV
                     YLEKSFDDTPWRPKERLKNAVELGDTSLMFLVHPTLTDDEIAFCKEHIEAVLAEATR"
     gene            1341..3251
                     /gene="pglD"
                     /locus_tag="WJL87_000679"
     CDS             1341..3251
                     /gene="pglD"
                     /locus_tag="WJL87_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ7799141.1"
                     /translation="MTLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSDEW
                     FDFANWQSFLLTALLTITLFIRMGLYRAVTRFVSFRILSTALAGSIVSAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGRSGRQLLEV
                     VKQMREYSAAAFVDDDPKLWHTVICGLAVYRPDDISALIERYGVEKILLAIPSATQEQ
                     RRRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDNRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAIEKELRETCIQKRLN
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATASGVETFVLISTDKAVRPTNTMGAGKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPLTLTHPEITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPNTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TAHPRIMTADEAMLPWHELSVLLDRIRAACDRYDQQAIRTLLINAPTGFTPSDGICDL
                     LWVRETHRKNAV"
     gene            3307..4599
                     /locus_tag="WJL87_000680"
     CDS             3307..4599
                     /locus_tag="WJL87_000680"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ7799142.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDQAFADIFSKLAAEHAVENIGNYSNPQGDAVLIDALTAFLNREYSWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKEGKIGAICCSRPTNPTGNVLTDSEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(4651..5487)
                     /locus_tag="WJL87_000681"
     CDS             complement(4651..5487)
                     /locus_tag="WJL87_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ7799143.1"
                     /translation="MIDLHCHSIVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKRGISGAYDGALALAANKEMVSRTHVAEFLIRVGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKKLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGLLASAGSDFHRLNDFSGGILGACPELPENCKPVWTHFS
                     RH"
     gene            complement(5797..6198)
                     /locus_tag="WJL87_000682"
     CDS             complement(5797..6198)
                     /locus_tag="WJL87_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799144.1"
                     /translation="MAIYDLNEIVTSAALGMYKGKWQNMDFEQVLQDIVQETDFTYSW
                     EYGENWAIFCLNEKSVGMLCKQIPFAIIENEKLSQKIYQEYPWILTQQVYHFDTDKIL
                     LNKYVCKLTIGIMSAISQEPISLQDLYVDTV"
     gene            complement(6303..6506)
                     /locus_tag="WJL87_000683"
     CDS             complement(6303..6506)
                     /locus_tag="WJL87_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799145.1"
                     /translation="MDTTLKNKAEAILGEPLLDEPIRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWSDDAV"
     gene            complement(6484..6717)
                     /locus_tag="WJL87_000684"
     CDS             complement(6484..6717)
                     /locus_tag="WJL87_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ7799146.1"
                     /translation="MEATVYLEDNEYIALCDLLKLAGLAESGGQAKAFIAEGLVLRNG
                     ETETRKTAKIRGGEVIEFDGARLEIADGYDPEE"
     gene            6883..10317
                     /gene="dnaE"
                     /locus_tag="WJL87_000685"
     CDS             6883..10317
                     /gene="dnaE"
                     /locus_tag="WJL87_000685"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ7799147.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRSAGIKPVGAADVWIGNPDAPDKPFRAMLIIRNDAGYLRLSELLT
                     AAYVGKDRNVHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLVEELGLPVVATHPTQFMSRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPSQFFIPPETMAERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDYLIKLSNEGLQERMVQLYPDEAERAEKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAEAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLGLDDAMKAEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIIDVGKIPLDDQVAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPNMKIRYALGAIKGTGEAAVESITAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPQKLSK
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRILANQVMTLQTARERYARSLSLALAPHHDIGGLVQLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            10465..10614
                     /locus_tag="WJL87_000686"
     CDS             10465..10614
                     /locus_tag="WJL87_000686"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799148.1"
                     /translation="MHDTDSTKGKNGIFQSIGIKTALSLQKYQIDKPGKEIRPCRLKK
                     RPPVR"
     gene            complement(10751..11683)
                     /locus_tag="WJL87_000687"
     CDS             complement(10751..11683)
                     /locus_tag="WJL87_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ7799149.1"
                     /translation="MLSQLEYGIAKENRPNRQADRFAGSVLPLAASLTHKPNIMKPIP
                     TDTFQPAILPQAFETEIKSTCTGRIYRIQTATLGEMPSEGYPVLFVLDGEAFFPALFN
                     IMQSLMPNPVTRSNAPCLIVGIGYTTGSVRDLAQRAADYTPPLGDNATADERQQFGQA
                     DRFAAFIDSELTAFLESRYTLNRNETAVFGHSFGALFGLYSLLSHRRFRRHWLVSPSI
                     WWHNRRILDFMPSENRLNGIDVCLNIGALERGSDCKRREERDMAGQAEQMAAELDRHG
                     AAVFFREYPNADHGNVPFYSLTDCVEYLRKAWQR"
     gene            complement(11753..13909)
                     /locus_tag="WJL87_000688"
     CDS             complement(11753..13909)
                     /locus_tag="WJL87_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ7799150.1"
                     /translation="MSVFRINMTAATVLAALSSSVFAAQTADLETVHIKGQRSYNAIA
                     TEKNGDYSSFAATVGTKIPASLREIPQSVSIITNQQVKDRNVDTFDQLARKTPGLRVL
                     SNDDGRSSVYARGYEYSEYNIDGLPAQMQSINGTLPNLFAFDRVEVMRGPSGLFDSSG
                     EMGGIVNLVCKRPAKAFQGHAAAGFGTHKQYKAEADVSGSLNSDGSVRGRVMAQTVGA
                     SPRPAEKNNRHETFYAAADWDINPDTVLGAGYLYQQRHLAPYNGLPADANNKLPSLPQ
                     HVFVGADWNKFKMNSHDVFADLKHYFGNGGYGKVGMRYSDRDADSNYAFAGSKLGMKT
                     LAGRPGCNTADDKACAVGLGTEIKQKALAFDASYSRPFRLGNTANEFVIGADYNRFRS
                     TNEQGRTTLYARGGLALNEFRSIPQVDLIANARKGVRGYSHTVATENLDEFGIYGKST
                     FHPADGLSLIGGGRLGHYKIELGEGKTLHKASKTKFTGYAGAVYDLNDNNSLYLSLSQ
                     LYTPQTNLDADGKLLKPRQGNQFEVGYKGSYMDDRLNARVSFYRMKDKNAAAPLNPNN
                     KKTRYAALGKRVMEGVETEISGAVTPKWQIHAGYSYLHSQIKTASNSRDDGIFLLMPK
                     HSANLWTTYQVTPELTIGGGVNAMSGITSSAGMHAGGYATFDAMAAYRFTPKLKLQIN
                     ADNIFNRHYYARVGGANTFNIPGSERSLTANLRYSF"
     gene            14069..14728
                     /locus_tag="WJL87_000689"
     CDS             14069..14728
                     /locus_tag="WJL87_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEZ7799151.1"
                     /translation="MTTPKLIIFDWDGTLADTTQPIIDTMRRSFAECGFPPPEAERVR
                     SLIGYSLPEIIRDLLEMPSETAVADIARTYSAHYLNPNNRNMTLFPDALPCLDKLKAQ
                     GFRLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGICGELGFDPK
                     EALVVGDTSHDLHMAANAGAAAVGVATGAHSREQLLSAPHLAVLDGLSELPGFLARHY
                     A"
     gene            complement(14815..15783)
                     /gene="ispH"
                     /locus_tag="WJL87_000690"
                     /gene_synonym="lytB"
     CDS             complement(14815..15783)
                     /gene="ispH"
                     /locus_tag="WJL87_000690"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ7799152.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVRQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(15812..16309)
                     /locus_tag="WJL87_000691"
     CDS             complement(15812..16309)
                     /locus_tag="WJL87_000691"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ7799153.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKT
                     QEEKY"
BASE COUNT         3810 a         4777 c         4295 g         3660 t
ORIGIN      
        1 ccccgtacag accgattttg acaacacctg cggcgcgcgc ccgattcttc ggaacacgcc
       61 ccccttcaga cggcataggg tcggaaatgc cgtctgaaaa ccgacggaca aaccatcatg
      121 ctgaacactt tcctttcccc gtggccctgc ttcacccaag aagaagccga tgccgtttcc
      181 aaagtcctgc tgtccaacaa agtcaactac tggacgggca acgaatgccg cgaatttgaa
      241 aaagaatttg ccgcctttgc cggcacgcgg tacgccgtcg cccttgccaa cggcacgctg
      301 gcactcgatg tcgcgctcaa agcaatcggc ataggcgcgg gcaacgatgt gattgttacc
      361 tcgcgcactt tcttggcttc cgcttcctgc attgtgaacg cgggcgcaaa tcccgtgttt
      421 gccgatgtgg atttgaacag ccaaaacatc agcgccgaaa ctatcaaagc cgcgctgacg
      481 tcgaatacca aagccatcat cgtcgtccac ctcgccggta tgcccgccga aatggacggc
      541 atcatggact tggcaaaaga acatgatttg tgggtcatcg aagactgcgc ccaagcgcac
      601 ggcgcgaaat acaaaggcaa atccgtcggc agcatcggac acgtcggcgc atggtcgttc
      661 tgccaagaca aaatcatgac cacgggcggc gaaggcggca tggttacgac caacgacaaa
      721 accctgtggg aaaaaatgtg gtcgtacaaa gaccacggca aaagctacga tgccgtgtac
      781 caccgcgaac acgcgcccgg tttccgctgg ctgcacgaaa gtttcggcac aaactggcgt
      841 atgatggaaa tgcaggcggc aatcgggcgc atccagctca aacgcctgcc cgaatggact
      901 gcctgccgtc aagcgcacgc cgccaagctg gcggaaagcc taggcaaatt caaaagtatc
      961 cgcttggttg aagtcgccga ctacatcgga cacgcgcaat ataagttcta cgctttcgtc
     1021 aaacccgaac acctcaaaga cggctggacg cgcgaccgca tcgtcagcga actgaacgcg
     1081 cgcaaagtcc cctgctatca aggcagctgc tccgaagtct atttggaaaa atctttcgac
     1141 gacacgcctt ggcggccgaa agagcgtttg aaaaatgctg tcgaactggg cgacaccagc
     1201 ctgatgttct tggtgcaccc gacgctgacc gacgacgaaa tcgcgttctg caaagaacac
     1261 atcgaagccg tattggcaga agccacacga taaccattca gacggcatat gccgtctgaa
     1321 aaatacacgc cgccgacaac atgactctgg aaaccctgat cgccctgccg cgcaacatca
     1381 agaaaatctg tttcctcata cacgattttc tgatgatttt cattgccttt tggttcaccc
     1441 aaagcctaaa ggccgactac tcggacgaat ggttcgattt tgccaactgg cagtcttttt
     1501 tgctgactgc cttgctgacc atcacattat ttatccgaat ggggctttac cgcgccgtta
     1561 cacgcttcgt cagcttccgc atcctctcca ccgcgctggc aggcagcatt gtttccgccg
     1621 tcctgttctt cctcaacaca ttgatttttg aagaaaggct gcgcctcgcc ctgccgattg
     1681 tctatttctt actgctgttt gtttccgtga ccggctcgcg tatggttttg cgcggactgt
     1741 tgtccgaaca ccccaaaaaa cagatgatcc ccgtcattat ttacggcgcg gggcgttcgg
     1801 gcagacagct tctcgaggtc gtcaaacaaa tgcgcgaata ttccgccgcc gcctttgtgg
     1861 acgacgaccc caaactgtgg cacaccgtca tctgcggcct tgccgtttac cgccccgacg
     1921 acatctccgc cctcatcgaa cgctacggcg tggaaaaaat cctgcttgcc attcccagcg
     1981 cgactcagga acaacgccgc cgaatcatca acaaactgga agcctatccc tgcgaagtgt
     2041 tgaccattcc cggaatgaaa gacctgatgg acgggaaaat cagcatcggc acgctcaaaa
     2101 aaatctccgt gtccgacctg ctcgggcgtg attccgtcgc gcccgacaac cgcctcatga
     2161 gtgccgacat cgaaggcaaa accgtcatgg taaccggcgc gggcggctcc atcggttcgg
     2221 aactctgccg ccagattatc cgccgccgcc ccgaaaagct gctgctgttc gagttatccg
     2281 aattcgccct gtacgccatc gaaaaagaat tgcgcgaaac ctgcatccaa aaacgcctca
     2341 acaccgaaat cctgcccttt ctcggttcgg tgcaaaaccg caccctgctc gaacacgtca
     2401 tgaccgcctt ttccgttgcg accgtctatc acgccgccgc ctacaaacac gtccccatgg
     2461 tcgaattcaa caccgtcgaa ggcatacgca acaacatctt cggcacactc gaatgcgcgc
     2521 ttgccgccac ggcttcgggc gtggaaacct tcgtcctcat ctccaccgac aaagccgtcc
     2581 gccccaccaa cacgatgggc gcgggcaaac gcatggcgga actctgcctt caggcactcg
     2641 ccgccgaacc cggacaaaaa acccgcttca gcatggtacg tttcggcaat gttttaggtt
     2701 cgtccggctc cgttgtcccg ctgtttgaaa aacagattgc agaaggcggc ccgcttaccc
     2761 tgacccaccc cgaaatcaca cgttatttca tgaccatacc cgaagccgcc caactcgtca
     2821 tacaggcagg cgcgatgggt acgggcggcg acgtattcgt cctcgacatg ggtgaatccg
     2881 tcaaaatcat cgaccttgcc cgccaaatga ttaccctaag cggcctcaaa cccaacacac
     2941 ccgaacaacc cgacggcgac atcgaaatcc tcattaccgg actgcgtccc ggcgaaaaac
     3001 tctacgaaga gctgctcatc ggcgacaacg tccgcaaaac cgcccatccg cgcattatga
     3061 cggctgacga ggcgatgctg ccgtggcacg agctctctgt cctgctcgac cgcatccgtg
     3121 cggcctgcga ccgttacgac cagcaggcaa tccgcaccct gctcatcaac gccccgaccg
     3181 gctttacccc aagcgacggt atctgcgacc tgctttgggt acgagaaaca cacagaaaaa
     3241 atgccgtctg aaccttcaga cggcataacg tacaaaccaa cctaccttac acacacggag
     3301 tttgacatgc agttttcagc attcggcgaa aaattcacgc aacacagcgg catcctccaa
     3361 ctgatggacg acctcggcga cgcgctcaaa agcgacaagc ccgtcaacat gctcggcggc
     3421 ggcaacccgg cgcgcattcc ggaaatcgat caggcgttcg ccgacatatt ctccaaactg
     3481 gcggcagaac acgccgtcga aaacatcggc aactactcca atccccaagg cgatgccgtg
     3541 ctgattgacg cgctgaccgc cttcctcaac cgcgaataca gctggaatct gaccgccgac
     3601 aatatcgcgc tgaccaacgg ttcgcaaaac gcgtttttct atttattcaa cctcttcggc
     3661 ggcaaattca agctttcaga cggcacatcc gcagaaaaag ccattttgtt gccgctcgcg
     3721 cccgaataca tcggctatgc cgacgtgcat gtcgaagggc agcacttcgt ttccgtcaag
     3781 cccaaaatcg aaaacgtcga acacgaaggc gaagcaggct tcttcaaata ccgcgtggac
     3841 tttgacgcac tggaaaacct gcccgaactg aaagaaggca aaatcggcgc gatttgctgt
     3901 tcgcgcccga ccaacccgac cggcaacgtg ctgaccgaca gcgaaatggc gcggctggac
     3961 gctttggcgc gtgaacacgg gattccgctg attatcgaca acgcctacgg aatgccgttc
     4021 cccaacatca tttacagcga cgtgacgctg aattggcacg aaaacatcat cctctgcttc
     4081 agcctttcca aagtcggcct gccgggcgtt cgcaccggca tcatcgtcgc cgcgcccgaa
     4141 gtcgtcaaag ccgtcagcag cctgaacgcg attgtaaacc ttgcccccac acgcttcggc
     4201 gcggccatcg ccgccccgct actggaaagc ggcgagatga aacggcttgc cgaccaagtc
     4261 atccggccgt tttaccgcaa tcaggcgcaa accgccgtct cgctgctcaa gcgcgagctg
     4321 ggcgcgtacc cgatgaaaat ccacaaaccc gaaggcgcga ttttcctgtg gctctggttt
     4381 gaaaacctgc ccgtttcttc gcaaaccctg tacgaaatgc tcaaagccga aggtacgctg
     4441 attattccgg gcgaacattt cttcgtcggc atcgacacgc aggattaccc gcacgcgggc
     4501 gagtgcatcc gcatgagcat cgcgcaggac gctcaaacgc tggaaaaagg cattgccgcc
     4561 atcggcaaaa ccgtccgaaa actgtacgac aacgtttaaa ccgcaaaaaa tgccgtctga
     4621 aaagttttca gacggcattt ttatccgcat tcaatgtcgg gaaaagtgcg tccacaccgg
     4681 tttgcagttt tccggcagtt cgggacacgc gccgagaatg ccgccgctga agtcgtttaa
     4741 acggtggaag tcgctgcccg cgcttgccaa caatccgaag cgttctgcca aaagcgcgta
     4801 gttgaggcgg tcgtttttgc aacagtttcc gctgtggact tcgatacccg caccgccgag
     4861 ttttttaaat tcttcaaaca gattgcgctt ggcggtggcg gacaaatcat aacgcatcgg
     4921 atgggcgatg accgccaacc cgcccgcgcc gttgacggcg gagacgcagt cttccagcgt
     4981 cgcccattcg tggcggacgg cgcaggattt gccgtcgccc aagtatttgg tgaacgcctg
     5041 ctgcttgttt ttgacgtgtc ccacccgaat gaggaactcg gcgacgtgtg tgcggctgac
     5101 catttctttg tttgccgcca gcgcgagcgc gccgtcgtat gcgcccgaaa tgccgcgttt
     5161 ttccagcttg gcggcgatgg cttcgaggcg tttcagacgg ccttgccgca cttgcgccaa
     5221 caggttttgc aggttttcgt cttgttcgtc aaagtctaag ccgacaacgt gtatggtacg
     5281 cccgcgccac gttacggaga tttccacgcc gttaatcagg cgcaaaccga gcctgtcggc
     5341 ttcggcacgc gcttcggcga tgccgccggt gtggtcgtgg tcggtcaacg ccagcagcgt
     5401 gcagccgttt tgatgcgcga ggcgcacgac ttcggcgggg gaaagcatac cgtcggaaac
     5461 gatggaatgg cagtgcaggt ctatcatggg gtgttatgtg tgtggtgaat gaaggtcggg
     5521 atgtttcaat gttgttgggc agtgtggcgg cggcggtcat tttaaaaaac aggtgtatgg
     5581 caaatgcaaa ggaaaagccg ctatgccgtc attcccaacc cccaaatgta cggatagagc
     5641 cagtagggat gggtacatta tttgtttgca ttattagtta tcctgctgta tccattaaat
     5701 catatgcttc aattaattgt tgttctagct cttgtaccaa ttttggattg cgaattccta
     5761 acgcaatttc aaacagcgag ttctcactat gtacatctac acagtatcta cataaagatc
     5821 ttgtaaagat ataggttctt ggctgatggc actcattatt ccaatagtta atttgcatac
     5881 atatttgttt aataaaattt tatctgtatc aaaatgataa acttgttgag ttagtatcca
     5941 agggtattct tggtatattt tctgtgataa tttttcattc tctataatcg cgaaaggtat
     6001 ctgtttacat aacataccaa cagacttttc gtttagacag aaaatagccc aattttcccc
     6061 atattcccaa gagtatgtaa aatctgtttc ctgaacaata tcttgtaaaa cttgctcaaa
     6121 atccatgttt tgccatttac ctttatacat acctaatgcg gcactggtta ctatttcgtt
     6181 taagtcataa atagccattt atgttccttg ctaatttcta ggtgagacat gtataggact
     6241 gagaccagta tgcccattag gttcccctct atacacatta atagtagatg ttggttccgt
     6301 tttcagacgg catcgtccga ccaacccgct tctttcaatt tccgccgttg cgcgtcgtag
     6361 cgggccttat ccgcccaata aatcgtctga atgcacgcct cgccgcagtc gctgccgcaa
     6421 cattcccacg actcggggcg gataggttcg tctaaaagcg gttcgcccaa gatggcttcg
     6481 gctttattct tcagggtcgt atccatcggc aatttccaag cgtgcgccgt caaattcaat
     6541 cacttcgccg ccgcgtattt tggcggtttt acgggtttcg gtttcgccgt tgcgcaacac
     6601 cagcccttcg gcgataaacg cttttgcctg tccgccgctt tcagcaagac cggccagttt
     6661 caacaggtcg cataaagcga tgtattcatt atcttcaaga tagactgttg cttccatcat
     6721 tcaatccttt tcaataaggg cgatatttta acatggccgt ctgaaagaaa gcccccttct
     6781 gtttgatttg ctttatccaa ccccattaca tttgattttc agacggcctt tagctgccaa
     6841 ccctgctaca atacgccttt tattgttcac gccgattttg ccatgaccga gccgacctac
     6901 atccccctgc gcctgcatac cgaattttcg attaccgacg gtatggtgcg gattaaaaaa
     6961 ctgattgcca aagcgcagga atacggtttg cctgctttgg gcatcagcga tttgatgaac
     7021 gaattcggtt tggtgaaatt ttataaagcc tgccgcagcg cggggattaa gcccgtcggc
     7081 gcggcggacg tatggatagg caatccggat gcgcccgaca agccgttccg tgctatgctg
     7141 attatccgca acgatgcggg ctatctgcgc ttgagcgagc ttttgacggc ggcttatgtc
     7201 ggcaaagacc gcaatgtcca tcatgcggaa ctcaatcccg aatggctgga aaacggcgac
     7261 aacagcggct tgatttgttt gagcggcgcg cattacggcg aagtgggcgt gaatctcttg
     7321 aacggcaacg aagacgcggc gcgggcggcg gcgttgaaat atgcggcgtg gtttcccgat
     7381 gcgttttatc tggagttgca acgcctgccc gagcgtcccg aatgggaggc ttgcgtttcg
     7441 ggcagcgtga agctggtgga ggaactgggt ttgccggtgg tggcgacgca tccgacgcag
     7501 tttatgagcc gcgacgattt caacgcgcac gaggcgcggg tgtgtatcgc aggcggctgg
     7561 gtattgacgg acaagaaacg tccgcgcgat ttcacaccga gccagttttt cattccgccg
     7621 gaaacgatgg ccgaacgttt cgccgattta cccgaagccc tcgaaaacac ggtggaaatt
     7681 gccaaacgct gcaatctgca catcacattg ggcaaaaact tcctgcccct tttccccacg
     7741 cccgacggtt tatcactcga tgactatctc atcaaactct ccaatgaggg cttgcaggaa
     7801 cgcatggttc agctttatcc cgacgaggcg gagcgtgcgg aaaaaatgcc ggaatatcag
     7861 gaacgtttgg attttgagct gaacatcatc atccagatga aattccccgg ctatttcctt
     7921 atcgtacaag actttatcaa ctgggcgaaa acacacggct gcccggtcgg gccgggacgc
     7981 ggttcgggcg cgggttcgct ggtggcgtat tcgctgaaga ttaccgacct cgatccgctg
     8041 aaatacgcgc tgctgttcga gcgtttccta aaccccgaac gcgtttctat gcccgacttc
     8101 gacgtggact tctgtcaggc aaaccgcggc cgcgtgattg aatatgtacg cgaaaaatac
     8161 ggtgcggaag cagtcagcca gattgttacc ttcggcacga tgtcgtccaa agcagtgatt
     8221 cgcgacgtgg ggcgcgtact ggaactgccg tttatgctgt gcgacaaact gtccaagctg
     8281 attccgttgg aagccaacaa acctttgggt ttggacgacg cgatgaaggc ggagccgcag
     8341 attcaggaat taatcgaagc ggaagaagcg gacgaactga ttacgctggc gaaaaagctg
     8401 gaagatttga cgcgcggttt gggtatgcac gcgggcggcg tgttgattgc gccgggcaag
     8461 atttccgatt acagccccgt gtatcaggcg gacgaatccg cctcgcccgt atccatgtac
     8521 gacaagggcg acgtggaaga cgtgggtttg gtgaaattcg actttttagg tctgcgcaac
     8581 ctgaccatca tcgaaatggc gcagaacaac atcaaaaaca ccaccggcga catcatcgat
     8641 gtcggcaaaa ttccgcttga cgaccaggtt gcctaccaaa tcttccgcga tgcgaacacc
     8701 accgccgtgt tccagttcga gtcgacaggc atgaagaaaa tgctgaaaac ggcgcatacg
     8761 accaagtttg aagaactgat tgccttcgta tcgctctacc gacccggccc gatggacaat
     8821 attcccgact tcgtcgcgcg tatgaaaggt caggaattcc aatacatcca cccgctactc
     8881 gaaggcatcc tcgcaccaac ctacgggatt atggtgtatc aggaacaagt gatgcaggcg
     8941 gcgcagatta tcggcggcta ctcgctcggc ggcgcggacc tgctgcgccg cgccatgggt
     9001 aagaaaaagc ccgaagaaat ggtgaaacac cgcgaaatct tcgccgaagg cgcggcaaaa
     9061 caaggcattt cgcgcgaaaa atccgacgaa atcttcaact acatggaaaa attcgccggc
     9121 tacggtttca acaaatccca cgctgccgcc tacgccctga tttcctacca gaccgcatgg
     9181 ctcaaagccc actaccccgc cgaatttatg gcggcgacca tgtcgtctga attggacaac
     9241 accgaccagc tcaagcattt ctacgacgac tgccgtgcca acggcatcga gttcctgccg
     9301 cccgacatca acgaatcaga ctaccgcttc acgccgtatc cgaacatgaa aatccgctac
     9361 gcgctcggcg cgattaaagg cacgggcgaa gccgccgtcg aatccatcac cgccgcgcgg
     9421 caaagcggcg gcaagtttac cggcctcttg gacttctgtg agcgcgtcgg caaagaacac
     9481 atgaaccgcc gcaccctcga agctctgata cgcggcggcg cattcgacag catcgaaccc
     9541 aaccgcgcca tgctcttggc gaacatcgac ctcgctatgg acaacgccga ccaaaaagcc
     9601 gccaacgcca atcagggcgg gcttttcgac atgatggaag acgccatcga accggtgcgg
     9661 ctcatcgacg cgccgatgtg gagcgaatcg gaaaaactcg ccgaagaaaa aaccgtcatc
     9721 ggcttttacc tgtccggcca cccgttcggc ccgtatgccc aagaagtccg ccaaatcgcc
     9781 ccgcagaaac tgtcgaaact caaaccgcaa gacagcgtgc gcctcgccgg attcgttacc
     9841 gccgtacgca ccatgatggg caagcgcggc aaaatcgcct tcgtcagcct tgaagatttg
     9901 agcggacagg ttgaaatcat ggtcggcggt cagacgttgg aaaactgcgc cgactgcctc
     9961 aaagccgacc aagtgctgat tatcgaatcc aaagtcagcc gcgacgacta cggcggcggc
    10021 gacggactgc gtattctggc aaaccaagtc atgaccctgc aaacggcacg cgaacgctac
    10081 gcccgcagcc tcagcctcgc cctcgccccg catcacgaca tcggcggact ggtacaactg
    10141 ctcgccgccc accaactgcc cgacacgccg cgcatcccgc tgcaactgtc gtatgccaac
    10201 gaaaaagcgt cgggcaggct tcaagtgccg ccgaaatgga cggttacacc gagctccgca
    10261 ttgttcggcg aactggaaac attgctcggc agccggtcgg tgcgcgtcaa ctggtaacca
    10321 aaaatagaaa tgccgtctga agcccaaaaa ccggtttcat tcgtacttta ttcgaatgat
    10381 tgaataaaag taactgccaa gaaaaacgta ttttttggtt atttcgccag tctaaataga
    10441 gcaaccggga cgattgatat ccgtgtgcat gacacagaca gcaccaaagg gaaaaacggc
    10501 attttccaaa gtatcggtat caaaaccgcc ctttcactcc aaaaatacca aatcgacaaa
    10561 ccgggcaaag aaatcagacc gtgccgcctg aaaaaacgcc cacccgtccg ttaaattaac
    10621 ctatccctgt ttcagacggc ctgaagcagg gatttttata tcaaaataaa atgagaaagg
    10681 gagcaataac ccttaggtag ctcttgttat tttccgatgc aaaacaaagc agtcatatat
    10741 ttaattcccc ctacctctgc caagccttcc tcaaatattc gacgcaatcg gtcagcgagt
    10801 agaacgggac attgccgtgg tcggcattcg gatattcccg gaaaaagacg gcggccccgt
    10861 gcctgtctaa ctctgccgcc atttgttcgg cctgccctgc catatcgcgt tcttccctgc
    10921 gtttacaatc gctaccccgt tctagcgcgc cgatgttgag gcagacatcg atgccgttta
    10981 gccggttttc agacggcata aagtcgagta tccgcctgtt gtgccaccaa atcgatgggg
    11041 atacgagcca atgccgtctg aaacggcggt gggaaagcag ggaatacagt ccgaacagtg
    11101 cgccgaacga gtgtccgaat acggcggttt cattgcggtt gagggtgtag cggctttcta
    11161 aaaaggcggt cagctcgctg tcgataaagg cggcgaagcg gtctgcctgt ccgaactgct
    11221 gccgttcgtc tgctgtggcg ttgtctccaa gcggcggcgt gtagtcggcg gcacgttgtg
    11281 ccaaatcgcg cacactgcct gtcgtgtagc cgataccgac aatcaggcag ggggcgttgc
    11341 ttcgggtaac ggggttgggc atcagcgact gcatgatgtt gaaaagtgcg gggaaaaagg
    11401 cttcgccgtc caaaacgaag aggacgggat agccttcaga cggcatttcg ccgagtgtcg
    11461 ccgtctgaat ccgatagatt cgccccgtgc aggtggattt gatttcggtt tcaaaggctt
    11521 ggggcagtat ggcaggttgg aatgtgtcgg tcggtatggg tttcatgatg ttcggcttgt
    11581 gggtcagact ggcggcaagc ggtaaaaccg aaccggcaaa gcggtctgcc tgccggttcg
    11641 gtctgttttc cttcgcaatg ccatactcca gttgtgagag catagggtat gcagcgcagc
    11701 ttgttgtggt ttgatgatgc tgcggctgcc gtttcagacg gcatattggt ctttaaaaac
    11761 tgtaacgcag gtttgccgtc aggctgcgct ccgaaccggg aatgttaaag gtgttcgcgc
    11821 cgccgacgcg ggcgtagtaa tggcggttga agatgttgtc ggcgttgatt tgcagcttca
    11881 gcttgggcgt gaagcggtat gccgccatcg catcgaacgt ggcataaccg cctgcatgca
    11941 tccctgcaga tgaagtaatg ccgctcatcg cgttcacgcc gccgccgatg gtcagctcgg
    12001 gcgtaacttg gtaagtcgtc cacaggtttg cgctgtgttt gggcatcaat aggaagatgc
    12061 cgtcgtcgcg tgaattggag gcggttttga tttggctgtg cagatagctg taacctgcat
    12121 ggatttgcca tttcggtgta accgcgccgc tgatttcggt ctcaacgcct tccatcacgc
    12181 gtttgcccaa tgcggcgtaa cgggtttttt tgttgttcgg gttcaacggt gcggcggcgt
    12241 ttttgtcttt catgcggtag aacgaaactc gggcattgag gcggtcgtcc atgtagctgc
    12301 ctttgtaacc gacttcaaac tggttgcctt ggcgcggttt gagcagcttg ccgtcggcat
    12361 cgaggttggt ttgcggtgtg tagagctggg acaggctcag gtagaggctg ttgttgtcgt
    12421 tcaagtcgta aaccgcgcct gcgtagccgg tgaacttggt tttgctggct ttgtgcaggg
    12481 ttttgccttc gcccaactcg attttatagt gtcccaaacg tccgccgccg ataagcgaca
    12541 gcccgtcggc aggatggaag gtggatttgc cgtaaatgcc gaattcgtcg aggttttcgg
    12601 tagcgacggt atggctgtaa ccgcgcacgc ctttgcgcgc gttggcaatc aaatcaacct
    12661 gcggtatgct gcggaactcg tttaaagcca ggccgccgcg tgcatataaa gtagtacggc
    12721 cttgttcgtt ggtgctgcgg aagcggttgt aatcggcgcc gatgacaaat tcgttggccg
    12781 tattgcccaa gcggaaaggc ctgctgtagc tggcgtcaaa cgcgagggct ttttgtttga
    12841 tttctgtacc caaacccacc gcgcaggctt tgtcgtcagc cgtattgcag cccgggcggc
    12901 ctgccagggt tttcatgccc agcttgctgc cggcaaaggc atagttggag tcggcatcgc
    12961 ggtcggaata gcgcataccg actttgccgt agccgccgtt gccgaagtaa tgtttcaaat
    13021 cggcaaacac gtcgtggctg ttcattttaa atttgttcca atccgcgccg acaaatacgt
    13081 gttgcggcag ggacggtaat ttgttattgg catcggctgg caagccgttg tacggcgcga
    13141 ggtggcgttg ctggtaaaga tagcccgcgc ccaaaaccgt atcggggttg atgtcccaat
    13201 ccgccgccgc gtagaaggtt tcgtgccggt tgtttttctc ggcgggacgc ggagacgcgc
    13261 cgacggtctg cgccatcacg cggccgcgca cgctgccgtc tgaattgagg ctgcccgata
    13321 cgtccgcctc ggctttatat tgtttgtgcg taccgaaccc tgccgcagca tgaccttgga
    13381 acgctttggc cgggcgtttg cacaccagat ttacgatgcc gcccatctcg ccgctgctgt
    13441 cgaacaagcc gctcggcccg cgcatcactt ccacacggtc gaaggcaaac agattgggca
    13501 gcgtgccgtt gatactctgc atctgcgcgg gcaggccgtc gatgttgtat tcgctgtatt
    13561 cgtaaccgcg cgcgtaaacc gaagagcgtc cgtcgtcgtt gctcaacacg cgcaggccgg
    13621 gcgttttgcg tgccaactgg tcaaacgtat caacattgcg gtctttgacc tgttgattgg
    13681 tgatgatgct gacagattgc ggaatttcgc gcaaagaagc ggggattttt gtgccgacgg
    13741 tggcggcaaa cgagctgtaa tcgccgtttt tctcggtggc aatcgcgttg tacgaacgct
    13801 gacccttaat atggacggtt tccaaatccg ccgtttgtgc ggcaaaaacc gaagacgaaa
    13861 gtgctgccaa aaccgtggcg gcggtcatat tgatgcggaa aactgacata aactgtccca
    13921 ttcacataaa tgataatggt tctattttaa taaagcacaa cgcggcttgt tcggaaaaac
    13981 atatcgcgca gccgacaaat tttgtcgaaa atgcgacacg tctgcgtttt ccgcataaaa
    14041 tctgactttt caccgtaaac aacctgccat gaccacgccc aaactcatca tcttcgactg
    14101 ggacggcacg cttgccgata cgacccaacc catcatcgac accatgcgcc gcagcttcgc
    14161 cgaatgcggt tttccgccgc ccgaagcgga acgcgtccgc agcctgattg gctacagcct
    14221 gcccgaaatc atccgcgacc tgctcgaaat gccgtctgaa accgccgttg ccgacatcgc
    14281 gcgcacttat tccgcgcatt acctcaatcc caacaaccgc aatatgacgc tctttcccga
    14341 tgccctgccc tgtctggaca agctcaaagc acaaggcttc cggcttgccg tcgccacggg
    14401 caaagggcgg gcgggtttgg acaacgccat cagccaaacc gccaccggcg gctattggct
    14461 cgccaccgcc tgcgcggggg aatatccctc caaaccctcg cccgaaatgg tattcggaat
    14521 ctgcggcgaa ctggggttcg acccgaaaga ggcattggtc gtcggcgata cgtcgcatga
    14581 cctgcatatg gcggcaaacg caggcgcggc ggcagtcggc gtggccaccg gcgcacattc
    14641 gcgcgaacag ctccttagcg caccgcatct cgccgtattg gacggtttgt ccgaactgcc
    14701 cggttttctt gcacgacatt acgcctgatt ggtttccgca tccggcacgc ggcaaaaatg
    14761 ccgtctgaag cctgttcaga cggcatttgt gttgcccaaa cattcaacgc ttgctcaacg
    14821 tttgcacaaa tcgggtttgg tttcgccctc gcggcgcagc tctttgggca ggacgaacac
    14881 aatgctttct tccgcaccct cgccttcgcg tacggtttcg tgcccccatc cgcgtatggt
    14941 tgccagtact tcccgcacca acacttcggg cgcggacgcg cctgccgtta cgccgacttt
    15001 gtttttgccc tcaaaccatg cgcgttgcag gtagcctgca ttatccacca tatacgcatc
    15061 gattccgcgc gatgccgcca cttcgcgcaa gcggttgctg ttggacgaat tgggcgaacc
    15121 gaccacaatc acgatgtcgc actgttccgc cagctctttg acggcggttt gccggttggt
    15181 cgtcgcatag cagatgtctt ccttgtgcgg attgcggata ttggggaaac gcgcgttcag
    15241 cgcggcgatg atgtctttgg tttcatcgac cgagagcgtg gtttggctga cataggcgag
    15301 tttgtcgggg tttctgactt cgagttttgc cacatctccg accgtttcga ccaaaagcat
    15361 tttgcccggc gcaagctgcc ccatcgttcc ttcaacctcg acgtgcccct tatgcccgat
    15421 catgatgatt tcacagtctt gggcatccag tcgggcgact tccttatgca ctttcgtcac
    15481 cagcgggcaa gtcgcatcaa acacgcggaa accgcgctcc gccgcttctt gccgcaccgc
    15541 cttcgatacg ccgtgtgccg aataaaccag tgtcgcgccc ggcggcactt ccgccaagtc
    15601 ttcaataaac accgcacctt tttcacgcag gttgtccacg acgaatttgt tgtgaacgac
    15661 ttcgtggcgc acataaatcg gcgcgccgaa ctcttccaaa gcacgttcga caatactgat
    15721 tgcccgatcc acaccagcgc agaagccgcg cggattggca aggatgatgg ttttctcgtt
    15781 cataagcccg gtatttcgtt ttcagacggc atcaatattt ttcttcttgg gttttacggt
    15841 ggacgatgtt gtccaacacc gccaacaccg caccgacgca gataaagctg tcggcaatat
    15901 taaaggcggg ataaaaccaa ttttgccaat aaaacaataa gaaatcgacg acatgaccgt
    15961 gtatcaggcg gtcgatgaca ttgcctaacg caccgccgat aatcattgcc gcacccgttt
    16021 tgccgagggt tgcaaactca tcgcgcaaga tggcgcgtac caaatacgcg ctcaccgcca
    16081 ccgccagcac caaaaaaaag tatttttgcc agccgccctg atcggcaagg aagctgaacg
    16141 ccgcacccgg gttgtacacc agcgtcagat cgaaaaagga aggaatgaca ttgacgcgtt
    16201 cccgatactg aaacgacgac agcaccgccc acttcgacca ctggtccagc acgatggcgg
    16261 caagtgccaa tacccaatag cgcgttttac ttgaaacaga tgaagacata tttttcaaca
    16321 gccggtaaaa gagtaccatt ttacccgaaa accccctttc ctgtacccga aacggcaaat
    16381 gccgtaatct taaaacccgt cattcccgac aacaccgtaa tctcgaaacc cgtcattccc
    16441 gcgtaggcgg gaatccagac ctgtccgcac agaaacttat cggataaaaa cagttgccca
    16501 aaccccgcgt tctatagtgg attaaattca aaccagtacg gc
//
LOCUS       DAWKST010000031        16536 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.031, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000031 DAWKST010000000
VERSION     DAWKST010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16536
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.031"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            208..1272
                     /locus_tag="WJL87_000692"
     CDS             208..1272
                     /locus_tag="WJL87_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ7799154.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1413..1736
                     /locus_tag="WJL87_000693"
     CDS             1413..1736
                     /locus_tag="WJL87_000693"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ7799155.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            1808..2131
                     /locus_tag="WJL87_000694"
     CDS             1808..2131
                     /locus_tag="WJL87_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ7799156.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2178..2381
                     /gene="tatA"
                     /locus_tag="WJL87_000695"
     CDS             2178..2381
                     /gene="tatA"
                     /locus_tag="WJL87_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ7799157.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2385..3071
                     /gene="tatB"
                     /locus_tag="WJL87_000696"
     CDS             2385..3071
                     /gene="tatB"
                     /locus_tag="WJL87_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ7799158.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKETGTDMQNSLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPFPDAANTLSDGISDVMPSERSDTSAEILGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRGLRPKHR
                     AKPKLRVRKS"
     gene            3085..3855
                     /gene="tatC"
                     /locus_tag="WJL87_000697"
     CDS             3085..3855
                     /gene="tatC"
                     /locus_tag="WJL87_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ7799159.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGLMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKIGVVTTEQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPATT"
     gene            3908..4516
                     /gene="maf"
                     /locus_tag="WJL87_000698"
     CDS             3908..4516
                     /gene="maf"
                     /locus_tag="WJL87_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ7799160.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYQVVKLPANIDETVRQNEDPARY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRSPLSALKP"
     gene            4525..4878
                     /locus_tag="WJL87_000699"
     CDS             4525..4878
                     /locus_tag="WJL87_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ7799161.1"
                     /translation="MNRQTAYFLASFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4868..6682
                     /locus_tag="WJL87_000700"
     CDS             4868..6682
                     /locus_tag="WJL87_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HEZ7799162.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALRLQPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPPISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACKSLVAHYGQERIVTLLAWSLLIGSLLQSCIVVIQFAGWEDTPM
                     LQNIIVYSGQGVIGHIGQRNNLGHYLMWGILAAAYLNGQRKIPPALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIYDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPAPDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6872..7549
                     /locus_tag="WJL87_000701"
     CDS             6872..7549
                     /locus_tag="WJL87_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ7799163.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7562..8968
                     /locus_tag="WJL87_000702"
     CDS             7562..8968
                     /locus_tag="WJL87_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ7799164.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIVVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9086..11512
                     /locus_tag="WJL87_000703"
     CDS             9086..11512
                     /locus_tag="WJL87_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEZ7799165.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSADWLNRFGHSLHTKTAVLHHNP
                     EENQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQSAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALEAADPALIAEAL
                     HLPPYRHTTHNAVQFQFGSHSLYGDILTARCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTIIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     YPKTLSLRNNTTVTITPILPEDAEAKQQFVRSLGPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLRTDHKIT"
     gene            11557..11802
                     /gene="rpsP"
                     /locus_tag="WJL87_000704"
     CDS             11557..11802
                     /gene="rpsP"
                     /locus_tag="WJL87_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ7799166.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11818..12327
                     /gene="rimM"
                     /locus_tag="WJL87_000705"
     CDS             11818..12327
                     /gene="rimM"
                     /locus_tag="WJL87_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ7799167.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTVSVTLEAGKVVNSELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKI
                     ITADWGLDY"
     gene            12327..13076
                     /gene="trmD"
                     /locus_tag="WJL87_000706"
     CDS             12327..13076
                     /gene="trmD"
                     /locus_tag="WJL87_000706"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ7799168.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQAAKVIYLSPQGKPLTHQKAVEL
                     AELPHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRSLIPKESRILNKILQEQREIQS"
     gene            13092..13457
                     /gene="rplS"
                     /locus_tag="WJL87_000707"
     CDS             13092..13457
                     /gene="rplS"
                     /locus_tag="WJL87_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ7799169.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13779..14504
                     /locus_tag="WJL87_000708"
     CDS             13779..14504
                     /locus_tag="WJL87_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ7799170.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFR
                     GINAVQKQRVHESLERVGMRQFAGRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPEWCAS"
     gene            14528..15403
                     /locus_tag="WJL87_000709"
     CDS             14528..15403
                     /locus_tag="WJL87_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ7799171.1"
                     /translation="MNLYDLLLAPFAEFDFMRYALVSVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSSLTLITLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15480..16394
                     /locus_tag="WJL87_000710"
     CDS             15480..16394
                     /locus_tag="WJL87_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEZ7799172.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSGDIKKIRSAKLILLNGLGLEAADIQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEFDPHVWNDPVLMSAYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNIKALTNAMKQ"
BASE COUNT         4310 a         5290 c         3644 g         3292 t
ORIGIN      
        1 cgtctgaagc cgattcggtt ttcagacgac atcattattt gacctgacgc aaacagaaag
       61 ccttccgcgt gcttaaacac atctcttgta gtaaaatatc taaacagatt caactggaaa
      121 cggtcaggct tcttcacgcc attcccattt caaccgccgc ctgccagatt cagacggtat
      181 tcctacttga aaacaaggag cgttacaatg aaagcagcac gtttttacga taaaggcgac
      241 atccgcatcg aagacatccc cgaaccgacc gtcgcccccg gcactgtcgg catcaatgtc
      301 gcctggtgcg gcatctgcgg tactgacctg cacgaattca tggaaggccc gattttcatt
      361 ccgccttgcg gtcatccgca cccgatttcc ggcgagtccg cacccgtcac gatgggacac
      421 gagttctccg gcgtggtcta tgccgtcggc gaaggcgtgg acgacatcaa agtcggccaa
      481 cacgtcgtgg tcgaacccta catcatccgc gatgacgtac cgaccggaga aggcagcaac
      541 taccacctct ccaaagatat gaactttatc ggcttgggcg gctgcggcgg cggtctgtcc
      601 gaaaaaatcg ccgtcaaacg tcgttgggtg catccgattt ccgacaaaat cccgttggat
      661 caagccgctt tgatcgaacc gctgtctgtc ggacaccacg cctatgtacg cagcggcgcg
      721 aaagcaggcg acgtcgcatt ggtcggcggc gcaggtccga tcggtttgct gttggctgcc
      781 gtgttgaaag ccaaaggcat caaagtcatc atcaccgagt tgagtaaagc acgcaaagac
      841 aaagcgcgcg aatccggcgt tgccgactac atcctcgacc cgtccgaagt cgatgttgtc
      901 gaagaggtga aaaaactgac caacggcgaa ggcgtggacg tggcattcga atgtaccagc
      961 gtgaacaaag tgttggatac tttggtcgaa gcctgcaaac ctgccgccaa tttggttatc
     1021 gtatccatct ggagccaccc tgcaaccgtc aacgtccaca gcgtcgtgat gaaagagctg
     1081 gatgtgcgcg gcacgattgc ctactgtaac gaccacgccg aaaccatcaa actggtcgaa
     1141 gaaggcaaaa tcaacctcga acccttcatc acccagcgca tcaagctgga cgaactggtt
     1201 tccgaaggct tcgagcgtct gatccacaac aacgaatccg ccgttaaaat tattgtgagt
     1261 ccaaacctgt aagcaaatta catttgcaac agcacaaatg ccgtctgaaa cgctcagacg
     1321 gcattttcca acgaagcgca acaggcgtat tgcccgagag cagccgttta gcctatcatt
     1381 ccgaacaaac agaaacaaac agaaggaaaa tcatgggaga ttccgtacta tccgccatcc
     1441 aacaaaccat cacccagcga aaatctgcca atccgtccga atcttacgtc gcacagctct
     1501 tgcataaggg cgaagacaaa atcctaaaaa aagtgattga agaggcgggc gaagtgttga
     1561 tggcatccaa agacaaaaac ccgtcccacc tggtttacga agttgccgac ttatggtttc
     1621 acaccatgat tcttctgaca caccacgacc tgaaggcgga agacgtattg gacgaacttg
     1681 cgcgccgcca aggtttgtcg ggcttggccg aaaaagccgc tcgcacagaa tcttgaattt
     1741 atattaaaat ccgcactttc ccacattcaa tccgtctgac cgctgttcag acggcaacgg
     1801 agccgttatg gacaactgta ttttctgcaa aatcgccgcc aaagacattc cggcgcaaac
     1861 cgtctatgaa gacggcgaaa tggtttgttt caaagacatc aaccccgctg ctccggttca
     1921 tctgctgctg attcccaaag tccatttcga ttcgttggca cacgccgcgc ccgaacatca
     1981 gccccttttg ggaaaaatga tgctgaaagt tcccgaaatc gccaaagcgg caggactggc
     2041 agacggcttc aaaaccctga tcaacaccgg aaaaggcggc ggacaagagg tcttccacct
     2101 gcatatacac atcatgggca cacccgtata aaccgttatt tcacaatcaa cccctaatac
     2161 ttacttaagg atacatcatg ggcagttttt ctctgacgca ctggattatc gtactgatta
     2221 tcgtcgtttt gatattcggc accaaaaaac tgcgcaacgt cggcaaagac ctcggcggtg
     2281 cggttcatga cttcaaacag gggctgaacg aaggtacaga cggcaaagaa gcccaaaaag
     2341 acgatgtaat cgaacacaaa aaagacgaag acaaagcgta atttatgttt gatttcggtt
     2401 tgggcgagct ggtttttgtc ggcattatcg ccctgattgt cctcggcccc gaacgcctgc
     2461 ccgaggccgc ccgcaccgcc ggacggctca tcggcaggct gcaacgcttt gtcggcagcg
     2521 tcaaacagga atttgacacg caaatcgaac tggaagaact aaggaaggca aagcaggaat
     2581 ttgaagctgc cgctgctcag gttcgagaca gcctcaaaga aaccggtacg gatatgcaga
     2641 acagtctgca cgacatttcc gacggtctga agccttggga aaaactgccc gaacagcgca
     2701 cgcctgctga tttcggtgtc gatgaaaacg gcaatccctt tcccgatgcg gcaaacaccc
     2761 tatcagacgg catttccgac gttatgccgt ccgaacgttc cgatacttcc gccgaaatcc
     2821 ttggggacag cgggcaaacc ggcagtacag ccgaacccgc ggaaaccgac caagaccgcg
     2881 catggcggga atacctgact gcttctgccg ccgcacccgt cgtacagacc gtcgaagtca
     2941 gctatatcga tactgctgtt gaaacgcctg ttccgcacac cacttccctg cgcaaacagg
     3001 caataagccg caaacgcggt ttgcgtccga aacaccgcgc caaacctaaa ttgcgcgtcc
     3061 gtaaatcata aagagagcaa tccggtgtcc gaaacacaaa acgaacaacc cgtccaaccg
     3121 cttgtcgagc atctcatcga gctgcgccgc cgcctgatgt ggacggttgt cggcatctta
     3181 gtctgctttt tcggcctaat gccgtttgcc caacaactct atacttttat cgccgacccg
     3241 ctgatggcaa acctgcccaa agacaccagc atgattgcca ccgatgtcat cgcaccgttt
     3301 ttcgtgccgg tcaaagttac cctgatggcg gcatttttaa tttcgctgcc gcatacgctc
     3361 taccaaatct gggcatttgt cgcgcccgca ctctaccaaa acgaaaaacg cctgattacg
     3421 ccgctcgtcc tctccagcgt cagcctgttt ttcatcggca tggcatttgc ctactttttg
     3481 gttttccccg tcattttcaa attccttgcc agcgttaccc ctgtcggtgt caatatggcg
     3541 acagacatcg acaaatacct ctccttcatc ttggggatgt ttgtcgcatt cggtacaacg
     3601 tttgaagtcc ccattgtcgt tatcctgtta accaaaattg gtgtggtaac aaccgaacag
     3661 ctcaaacgcg cccgccccta tgtgattgtc ggcgcgtttg tcattgccgc catcatcacg
     3721 ccgcccgatg tgatttcaca aaccctgctt gccattccgc tgattctctt atacgaagca
     3781 ggtatttggt tcggacgctt tttcacgcca cgttcagaac aggatggcga catacagccg
     3841 cctgcaacaa cctgacacta tgccgtccga acctccgcct cataccgcaa cagattaagg
     3901 aatacctttg aataccctct atttaggttc aaacagcccg cgccgaatgg aaatcctgac
     3961 acagttgggc tatcaggtcg tcaagctgcc tgccaacatc gacgaaacgg tcagacagaa
     4021 cgaagaccct gcccgttacg ttcaaaggat ggcagaagaa aaaaaccgaa ccgccctgac
     4081 cctcttttgc gaaaccaacg gcacaatgcc cgatttcccc ctgattaccg ccgacacctg
     4141 cgtcgtttca gacggcatta tattaggcaa accccgctcc caagccgaag caatcgaatt
     4201 tttaaaccga ttgtccggca aacaacatac cgtcctgact gctgtctgca ttcattatcg
     4261 cggcaaaacg tcaagccgcg tccaaaccaa ccgcgttgtt ttcaagcccc tgagttcgga
     4321 agaaatttcc gcctatgtgc aaagcggcga accgatggac aaagccggcg cctacgccgt
     4381 acaaggcata ggcggcatct ttatccaatc tatcgaaggc agcttcagcg gcattatggg
     4441 actgcccgtt tatgaaaccg tttcgatgtt gcaggatttg ggataccgct cccccttgtc
     4501 cgcccttaaa ccgtaaagac cgccgtgaac agacaaaccg cttacttcct tgcctccttc
     4561 agcctgatcg cactgataat cctgtccctt tcttgggaac tgtggattgc accgttacgc
     4621 ccgggcggct cgtggctcgc gctcaaagcc ctccccctct gtctgccgct ttcaggcatc
     4681 ttgaaaaaga aaatctatac ctaccaatac agctccatgc tggttctgat ttattttgcc
     4741 gaagccgtca tgcgcctgtt cgacgcctat ccggcagaac aaatttgcgc cgcgctttcc
     4801 gcagtattca gcatcatctt cttcatatcc tgcctgtcgt tcgtcaaaca atacaaggaa
     4861 acaaacaatg cccgctgaaa cgaccgtatc cggcgcgcac cccgccgcca aactgccgat
     4921 ttacatcctg ccctgcttcc tttggatagg catcgtcccc tttacctttg cgctcaggct
     4981 gcaaccgtcg cccgactttt accacgatgc cgccgccgca gccggcctga ttgtcctgtt
     5041 gttcctcacg gcaggaaaaa agctgtttga tgtcaaaatc ccacctatca gcttccttct
     5101 gtttgcaatg gcggcgtttt ggtatcttca ggcacgcctg atgaacctga tttaccccgg
     5161 tatgaacgac atcgtctctt ggattttcat cttgctcgcc gtcagcgcgt gggcctgcaa
     5221 gagtttggtc gcacactacg gacaagaacg catcgttacc ctgcttgcct ggtcgctgct
     5281 tatcggctcc ctgcttcaat cctgcatcgt cgtcatccag tttgccggct gggaagacac
     5341 ccccatgctt caaaacatta ttgtttacag cgggcaaggc gtaatcggac acatcggaca
     5401 gcgcaacaac ctcggacact acctgatgtg gggcatactc gccgccgcct acctcaacgg
     5461 acaacgaaaa atcccgcccg ccctcggcgc aatctgcctg attatgcaga ccgccgtttt
     5521 aggtttggtc aattcgcgca ccatcttgac ctacatagcc gccatcgccc tcatccttcc
     5581 cttctggtat ttccgttcgg acaaatccaa caggcggacg atgctcggca tagccgcagc
     5641 cgtattcctt accgcgctgt tccaattttc catgaacacc attctggaaa cctttaccgg
     5701 catccgctac gaaactgccg tcgaacgcgt tgccaacggc ggtttcacag acttgccgcg
     5761 ccaaatcgaa tggaataaag cccttgccgc cttccagtcc gccccgatat tcgggcacgg
     5821 ctggaacagt tttgcccaac aaaccttcct catcaatgcc gaacagcaca acatatacga
     5881 caacctcctc agcaacttgt tcacccattc ccacaacatc gtcctccaac tccttgcaga
     5941 gatgggaatc agcggcacgc ttctggttgc cgcaaccctg ctgacgggca ttgccgggct
     6001 gctgaaacgc cccctgaccc ccgcatcgct tttcctaatc tgcacgcttg ccgtcagtat
     6061 gtgccacagt atgctcgaat atcctttgtg gtatgtctat ttcctcatcc ctttcggact
     6121 gatgctcttc ctgtcccccg cagaggcttc agacggcatc gccttcaaaa aagccgccaa
     6181 tctcggcata ctgaccgcct ccgccgccat attcgcagga ttgctgcact tggactggac
     6241 atacacccgg ctggttaacg ccttttcccc cgcccctgac gacagtgcca aaaccctcaa
     6301 ccggaaaatc aacgagttgc gctatatttc cgcaaacagt ccgatgctgt ccttttatgc
     6361 cgacttctcc ctcgtaaact tcgccctgcc ggaatacccc gaaacccaga cttgggcgga
     6421 agaagcaacc ctcaaatcac taaaataccg cccccactcc gccacctacc gcatcgccct
     6481 ctacctgatg cggcaaggca aagttgcaga agcaaaacaa tggatgcggg cgacacagtc
     6541 ctattacccc tacctgatgc cccgatacgc cgacgaaatc cgcaaactgc ccgtatgggc
     6601 accgctgcta cccgaactgc tcaaagactg caaagccttc gccgccgcgc ccggtcatcc
     6661 ggaagcaaaa ccctgcaaat gacccgcgcc ggcggatacg gataccgccc gaaatgtaaa
     6721 gctccatgca agacattgca aaaaacagca aaccggtagg gaaatacgct atcaaaaaac
     6781 attgcagccg tgttaagata aaccgtcaaa caatcttttc acgccccgcc cgaaacaggg
     6841 tcggggcata cccttacgaa aaggaaacac catgagccgc gtattactcg tagatgacga
     6901 tgccctgctg accgaactgc tgaccgaata cctgagcgcc gaaggtctga acgtccgcag
     6961 cgttcccgac ggggaagcag gcgtacagga aatcctgagc gggcagtacg atgtagtcgt
     7021 attggattcc atgatgccca aaatgaacgg cttggatgtc ttgaaaaacg tacgcgcccg
     7081 aagcaccgtc cccatcatca tgctgaccgc caaaggcgac gacatcgacc gaatcatcgg
     7141 cttggaaatg ggcgcggacg actatgtccc caaaccctgc acaccacgcg aactcttggc
     7201 acgcatcaat gccatcctgc gccgcgcaca acacagcggc gaacagaaca acgcacccaa
     7261 cagcatctcc gtcagcgatg tcgtcctgta ccccgccaaa cgccaggcat ccgtcaaaga
     7321 catgccgctc gaactgacca gcaccgaatt caacctgctc gaagtcctga tgcgccatgc
     7381 cggacaggta gtcagcaaag aaaccctgtc cgtcgaagca ctcgaccgca agctggcaaa
     7441 attcgaccgc agtatcgacg tacacatctc cagcatccgc cacaagttgg gcgatgcctc
     7501 tctgattcaa accgtacgcg gcttgggcta cctgtttgtc aaaaactgaa gcaacgggca
     7561 aatgaaactg ttccaacgca ttttcgccac attttgcgca gttatcgtct gtgcaatctt
     7621 tgtggcgagt ttttcttttt ggctggtgca gaacaccctt gccgaaaacc agttcaacca
     7681 acgccgcacc atcgaaacca ctttgatggg cagcatcatt tccgcattcc gggcacgcgg
     7741 ggacgcgggc gcgcgcgaaa tcctgacgga atggaaagac agccccgtct catcgggcgt
     7801 gtacgttata cagggcgacg agaaaaaaga tatcctgaac cggtatatcg acagctacac
     7861 catcgaacgc gcccggcttt tcgccgccgg acacccgcat tccaacctcg tccatatcga
     7921 atacgaccgc ttcggcgaag aatacctgtt cttcaccaaa gactgggaca aactccaagc
     7981 ccgccgcctg cccagccccc tgttgatccc cggcctgccg ctcgccccga tttggcacga
     8041 actcatcata ttgtccttca tcatcgtcgt cggactgctg atggcgtaca tcctcgccgg
     8101 caatatcgcc aaacccatca gaatcttagg caacggcatg gacagggtgg caaacggaga
     8161 acttgaaacc cgtatctccc aacaggtcga cgaccgcgac gacgaattgt cccatcttgc
     8221 catccaattc gacaaaatgg tggaaaaact cgaaaaactc gttgccaaag aacgccacct
     8281 gctccaccac gtttcccacg aaatgcgctc cccgcttgcc cgtatgcagg cgattgtcgg
     8341 actgattcag gcgcagcccc aaaaacagga acaatatctc aaacggctgg aaggcgaact
     8401 gacccgcatg gatacgctgg ccggggaact gttaaccctg tcccgtctcg aaacttccaa
     8461 tatggctttg gaaaaagaaa gcctgaaact cctgcccttc ctgggcaacc tggtagaaga
     8521 caatcaaagc attgcccaga aaaacggaca aacggttacc ctgtctgccg acggaaaaat
     8581 ccccgaaaac acaaccatcc ttgccaacga aagctacctg taccgcgcct tcgacaacgt
     8641 catccgcaac gccgtcaact acagtcccga aggcagcacc atcctgatca acatcggaca
     8701 agaccacaaa cactggataa tcgacgttac cgacaacggc cccggcgtgg acgaaatgca
     8761 gctcccgcac atcttcaccg ctttctaccg tgcagactcc agtgccaaca aacccggaac
     8821 aggactgggg cttgcattga cccaacatat tattgaacag cactgcggca aaatcatcgc
     8881 cgaaaacatc aaaccgaacg ggctgcggat gcgcttcatc ctacccaaga aaaaaaccgg
     8941 ttccaaaaca gaaaaaagtg cgaactgacc ataatgccgt ctgaaccggc ttcagacggc
     9001 attgcacaaa cagttacccc aaaacaaaca cacagcgggt gcatctccaa aaatcacgca
     9061 cataccgtcc gcaaaggaat atatcatgtc ggcacaaacc gatcccggct acttcttcat
     9121 gcccaaccac attatcctga taggcgcgag cgaacgtccg tacagcctgg gcgaacgcgt
     9181 actcagcaac ctgctgagta cgccctttca aggaaaaatc acccccgtaa acccgcgcca
     9241 ccacaccata gccgggcttc ccgcctacac cagcctcaac aaaatccccg gcaatgcaga
     9301 cctgattatt gccgttaccc tacccgacag ttacgacacc ctcttcaaaa cctgccgcaa
     9361 aaagcagctc cgacacatca tcctcataca ggactgggac agcctgtctg ccgcagaact
     9421 gcacaccgcc gaaactgcca tccgcaaaca ccacggcaac ggactcaaca tcaccgcctg
     9481 caccaccgca ggcatccaac tgccctcact cggacttaac atcagtaccc aagacggata
     9541 cgccgcaggc cataccgcca tactgaccgg caatgccgcc gtcagccgcc aaatcgacaa
     9601 catcctgaac aaactccgtc aaggcacatc ccgccacatc agcctgcatc ccggcatcag
     9661 ccccatcaca tccgccgatt ggctcaaccg cttcggacac agcctgcaca ccaaaaccgc
     9721 cgtcctacac cacaaccctg aagagaatca gcgcaaactg ttcagcgcaa tccgccaatt
     9781 tacccgccat acgccgctga ttctccacat cacctgccgc acgacagaaa ccgaccgtgc
     9841 cgtactgcac tgcctcgccc gacactgcaa cttcctcatc agtttcaacg ccgacgacct
     9901 cgaagccgca ctgcgcgccc aactgtccga ccttccaccc ctgtcccgac tcgacatcct
     9961 gtccgacacg cctgccgaat ggctgaacgc gcacgcgcca aaaaccctcg ccctccactt
    10021 tcccaacctt ccccaccaca tccgcaacgg acacctgacc ggcacaccca caccctcaat
    10081 ctgccacgac atcgcctcac gtcagcttgc ccgccccgac acccaagccg tcctgaccat
    10141 cctcagcccc tccggacacg aggattacaa aaaaacagca cgcgccctta tccgcctgtc
    10201 cgaacagagc gccaaacccc tgctcgtcag cagccccttt tcagacggca taacacattt
    10261 cgacaccccc actcaggcaa tccgcaccct ttcctaccgc aacaccgccg ccgccctgaa
    10321 acaggcacag ctcgacattg caccgccgca gccatgccgt ctgaaaacac cgcaacccca
    10381 aaacatcaaa aaagccctcg aagccgccga ccccgcccta atcgccgaag ccctgcacct
    10441 ccccccctac cggcacacca cccataacgc cgtccaattc caattcggca gccactccct
    10501 ctatggcgac atcctgaccg cacgttgcaa cggacaaacc actgccgtac tcccgccgtt
    10561 taccacgctc gacagccgcc accttgcccg ctttgccgaa ctcgacggca cacaaaccct
    10621 cgaccagttc ctgcacacac tgaccatcat tcccgaatac cgccaacaca ttctcggcat
    10681 caccctcaac ctaaacggcg gacaatacag cagcgatttc atcttaaaga cacccgaaac
    10741 acacgacacg cccaaacgca agaacacagg caaagccgcc caaaccctcg aacatgccgc
    10801 cgcaaaaatg cagagtgccg ccgcatacct gaaacacaaa aacccgacag ccgccgaatt
    10861 tctccgccac acaagcgaag ccgccgcaga actgctcggc agcaaaaccg aaaccggagc
    10921 agccgtaccc aacgtacttg ccccctatcc cgcagcatac cccaaaacac tgtccctaag
    10981 aaacaacacg accgttacca ttacccccat tttgcccgaa gacgcagaag ccaaacagca
    11041 gttcgtccgc agcctcggtc ccgaagcacg gtacacacgc ttcatgaccc acaccaacga
    11101 actgcccgca gccacgttgg cacgcctgtg caaccccgat taccactgtg aagccgcatg
    11161 gacggcaaag gatgccgaca gcaacatcgt cgccgtcgtc cgccacagcc gcctgaaccg
    11221 caacgaatgc gaattcggca tcacactggc ggaacatatg cgcggcagcg gactggcaca
    11281 gaaaatgatg gaactcatca tccaaaccgc cgcacagcaa ggctaccgga ctatgagtgc
    11341 cgacattctc aaaaccaata cccccatgat caaacttgct gaaaaatcag gatttaccct
    11401 caaggaatcg gacaccgaaa aaaacctgta ccgcgcatat ctgaaccttg cggcagacaa
    11461 aacaacagaa aaaacaaata aaaacttgcg caccgaccac aaaataacct aaaatcggca
    11521 gtttccatat atccgatttt ctcaaaagga ctcaaaatgg tagttatccg tttggcacgc
    11581 ggcggctcga aacaccgccc cttctacaac gtcatcgtta ctgactcacg cagccgccgc
    11641 gacggccgct tcatcgaacg cgtaggcttc tacaaccccg tagccaacga aaaacaagag
    11701 cgcgtccgcc tcaatgcaga ccgcctgaac cactggattg cacaaggcgc gcaagtcagc
    11761 gactccgttg caaaactgat taaagaacaa aaagccgcct aattcacatt tgccgccatg
    11821 acagacactc aaaaccgggt agccatgggc tacatcaaag gcgtattcgg cataaaaggc
    11881 tggttgaaaa ttgccgccaa caccgaatat tccgacagcc ttttggacta ccccgagtgg
    11941 catttggtca aggacggcaa aaccgtcagc gttacccttg aagccggaaa agtcgtcaac
    12001 agcgaactcc aagtcaaatt cgaaggcata aacgaccgcg acttggcatt ctcattgcgc
    12061 ggttacacca tcgaaatacc ccgtgaagca ttcgccccga cagaagaaga cgaatactac
    12121 tggacagact tggtcggcat gaccgtcgtc aacaaagacc ataccgtttt aggcaaggta
    12181 agcaacctga tggaaaccgg cgcaaacgac gtattgatga ttgacggaga acacgggcag
    12241 attctgattc cgttcgtttc ccaatatatc gaaaccgtcg ataccggcag caagattatt
    12301 actgccgact ggggtttgga ctactgatgc ttatccaggc agttaccatt ttccccgaaa
    12361 tgttcgacag cattacccgc tacggcgtaa cgggacgcgc gaacagacag ggaatctggc
    12421 agtttgaagc agtcaatccc cgaaagtttg ccgacaacag attgggctac atcgacgacc
    12481 gcccgttcgg cggcggcccg ggaatgatta tgatggctcc gccgcttcat gcagcaatag
    12541 aacacgccaa agcacaatcc tcccaagctg caaaagtcat ctacctcagc ccccaaggga
    12601 aaccgctgac acaccaaaaa gcggtagaac tggcagaact tccgcatctg attctgctgt
    12661 gcggacggta tgagggcata gacgaaaggc ttctgcaaag cagcgtcgat gaagaaatca
    12721 gcatcggaga cttcgttgtt tccggcggag agcttcccgc catgatgctg atggatgcgg
    12781 tattgaggct cgtacccggc gtattgggcg atatgcagtc tgccgaacaa gattcgttct
    12841 caagcggcat tttggactgc ccccactaca ccaaaccctt agaatttcaa ggcatggctg
    12901 ttccggaagt attacgttcc ggcaatcatg gcttgatagc ggaatggcgg ttggaacaat
    12961 cgctgcgccg caccttggag cgcagacccg atcttttgga aaagcgcagt ttaatcccaa
    13021 aggaatcccg catcttgaat aaaatcctac aagagcaacg ggaaatccaa tcataattta
    13081 ggaaaaaaac aatgaacctg attcaacagc tcgagcaaga agaaattgcc cgcctgaaca
    13141 aagaaatccc cgaattcgca ccgggcgaca ccgtagtcgt atccgtacgc gtcgtggaag
    13201 gtacccgcag ccgtctgcaa gcctacgaag gcgttgtcat tgcacgccgc aaccgtggtc
    13261 tgaacagcaa cttcatcgtc cgtaaaattt ccagcggcga aggtgttgaa cgtactttcc
    13321 aactgtactc tccgaccgtc gaaaaaatcg aagtcaaacg ccgtggcgac gtacgccgtg
    13381 ccaaactgta ctacctgcgc ggtctgaccg gcaaagctgc acgcatcaaa gaaaaactgc
    13441 ctgcacgcaa aggttgattc aaacccgttt tccccaatgc cgtctgaacc ttctgacggc
    13501 atttcttatt gccgcaacat ccgcaacgga acagcaccaa gaagcacacc ccttccggca
    13561 catcccgaat ccccgtccga accgaacaaa atttacagac caaacgttat acagtatcat
    13621 atcagccata taataataac cgctttaccg ccttcccttt caaaaccgca aatcatcatg
    13681 acaccttccc ttttactatc aggattgacc ttccgcctcg tccttgccct gattgccgta
    13741 tcccttttat ggggcgttta cttttgggca gtatccgcat gagcatcatt gtcgaaaacc
    13801 tgaccgtcag ctaccgccgc cgacctgccg tgcaccatgt ggacattact tttgaagaac
    13861 atagtatgtg ggcggttttc ggtcccaacg gcgcagggaa atccaccttt ctcaaatcct
    13921 taatgggatt gcagcctatc gatacgggca gcatccggct ggacggattg acccgtcaga
    13981 acattgccta ccttccccag cagtccgaca tcgaccgctc gcagcccatg accgttttcg
    14041 acttggcggc aatggggcta tggtatgaaa tcggtttttt cagaggaata aatgccgttc
    14101 aaaaacaacg cgttcacgaa tctttggaac gcgtcgggat gcggcagttt gccggccgcc
    14161 aaatcgccca cctctcaaac ggacaattcc agcgtgtcct ttttgcccga atgctggttc
    14221 aaaacgccaa attcctactt ctagacgaac ctttcaacgc tgttgatgca cggacgacct
    14281 acgaacttct cgacgtattg cagaaatgcc attgcggcgg acacgccatc atcgccgtac
    14341 tgcacgatta cgaacaagtc cgtgcctact ttcccaatac cctgctgctc gcccgcgaaa
    14401 aaattgcggc aggcgcaacc gagaccattc tgacagaacc ctacctcgcc caagccaacg
    14461 ccaaaatgca gcaacaggaa agccccgagt ggtgcgcgtc ataaatgccg tctgaaaccg
    14521 aaaaaccatg aacctctacg acctgctcct tgcccccttt gcagaatttg actttatgcg
    14581 ctacgccctc gtatccgtct tctgcctgtc cctcagtgcc gcacccgtcg gcgtattcct
    14641 cgtcatgcgc cgtatgagcc tgataggcga cgcattgagc cacgccgtcc tgcccggtgc
    14701 cgccgtcggc tacatgtttg ccggcttaag cctgcccgcc atgggtttgg gcggcgtagc
    14761 cgcaggtatg ctgatggcac tgcttgccgg actcgtcagc cgcttcacca ccctgaaaga
    14821 agatgccaac tttgccgcct tttatctcag cagcctcgcc atcggtgtag tcctcgtcag
    14881 caaaaacggc agcagcgtcg atctgctgca tctgcttttc ggctctgtac ttgccgtcga
    14941 tattcctgcc ctgcagctca tcgccgccgt atccagcctc acgctcatta cccttgccgt
    15001 catctaccgc ccgctcgtac tcgaaagcat cgaccccctg tttctcaaat ctgtcggcgg
    15061 caaaggcggg ctttggcacg tcctctttct cgtcctggtc gtcatgaacc tcgtatccgg
    15121 ctttcaagcc ctcggcacac tcatgtccgt cggactcatg atgctgccag ccattaccgc
    15181 ccgcctgtgg gcgaagcaca tgggcgcact catcctccta tccgttctga cagccctgct
    15241 gtgcggcttg agcgggctgc tcatttccta ccacatcgaa atcccttccg gccccgccat
    15301 catcctctgt tgcagcgtcc tttatctctt ttccgtcata ctcggcaaag aaggcggcat
    15361 tctgaccaaa tggctcaaaa accaccgcca ccacaccacc tgaccccgca agccgcttga
    15421 acaaacaggc ggcttgtttt accataacag atgttacgtt atatcacagg agaacagtta
    15481 tgaaacacct caaactcacc cttattgccg cattgctgac cactgccgca actgccgccc
    15541 cccttccggt tgtaaccagt ttcagcattt taggcgacgt tgccaaacaa atcggcggag
    15601 agcgcgtatc catacaaagt ttggtcggag ccaaccaaga tactcatgcc tatcacatga
    15661 ccagtggcga cattaaaaaa atccgcagtg caaaactcat cctgctcaac ggcttaggac
    15721 ttgaagccgc cgacatccaa cgtgccgtca aacagagcaa agtatcctat gccgaagcga
    15781 ccaaaggcat ccaacccctc aaagccgaag aagaaggcgg acaccatcat gaccacgacc
    15841 atgaccacga aggacaccac catgaccacg gcgaatttga cccgcacgtc tggaacgacc
    15901 ccgtcctcat gtccgcctat gcccaaaacg tcgccgaagc cctgataaag gccgaccccg
    15961 aaggcaaagt ttattatcaa caacgcttgg gcaactacca aatgcagctc aaaaaactgc
    16021 acagcgacgc acaagccgca tttaacgccg tccctgccgc caaacgcaaa gtcctgaccg
    16081 ggcacgatgc cttttcctac atgggcaaac gttaccatat cgaattcatc gccccgcaag
    16141 gtgtgagcag cgaagccgag ccttcagcca aacaagtcgc cgccatcatc cgacaaatca
    16201 aacgcgaagg catcaaagcc gtattcaccg aaaatatcaa agacacccgc atggttgacc
    16261 gtatcgccaa agaaaccggt gtcaacgtca gcggcaaact gtattccgac gcactcggca
    16321 acgcgcccgc agacacctac atcggaatgt accgccacaa catcaaagcc ttgaccaacg
    16381 cgatgaagca ataaaaccgt ttgattggtt tgaatttgaa aaggcaaaag gccgtctgaa
    16441 aattatagtg gattaacaaa aatcaggaca aggcgacgaa gccgcagaca gtacagatag
    16501 tacggtaagg cgaggcaacg ctgtactggt ttttgt
//
LOCUS       DAWKST010000032        16485 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.032, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000032 DAWKST010000000
VERSION     DAWKST010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16485
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.032"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(36..761)
                     /locus_tag="WJL87_000711"
     CDS             complement(36..761)
                     /locus_tag="WJL87_000711"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ7799173.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSRDLDAGRLLEAYRN
                     GVFPWFLRDGWFFWYAVGPRAVIVPERLHVPRSLEKTLRNCSYRVTVNGCFAEVVAHC
                     AAAVRPNQDGTWIAPEFQTAYLKLHEMGYAHSFECHYPDENGKTRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLASLGVELIDCQQDTGHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(831..1334)
                     /gene="fur"
                     /locus_tag="WJL87_000712"
     CDS             complement(831..1334)
                     /gene="fur"
                     /locus_tag="WJL87_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ7799174.1"
                     /translation="MIVGKQAEIAYYSVYYFTGILNIMEKFSNIAQLKDSGLKVTGPR
                     LKILDLFETHAEEHLSAEDVYRILLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGK
                     AVYELDKGDHHDHIVCVKCGEVTEFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSD
                     CQAKGKR"
     gene            1492..1869
                     /locus_tag="WJL87_000713"
     CDS             1492..1869
                     /locus_tag="WJL87_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ7799175.1"
                     /translation="MNKTLILVLSALFSLTACSVERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPMLRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RAEGDALQNAAEALRAKQNADKQ"
     gene            1879..2688
                     /gene="dapB"
                     /locus_tag="WJL87_000714"
     CDS             1879..2688
                     /gene="dapB"
                     /locus_tag="WJL87_000714"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ7799176.1"
                     /translation="MIPLKIAIAGVNGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDTVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIQAAGEKTGVVFAANFSVGVNLTFHLLDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKASSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2952..3362)
                     /locus_tag="WJL87_000715"
     CDS             complement(2952..3362)
                     /locus_tag="WJL87_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799177.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPTPNYTDWVKIKFKQFSYLKFIYGYATKNQDKNIKNVLELGELKQDDEILDYGGA
                     LEVIGSRYDLPTGFSIDIVCREIELEFLDQESFN"
     gene            3585..3974
                     /locus_tag="WJL87_000716"
     CDS             3585..3974
                     /locus_tag="WJL87_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ7799178.1"
                     /translation="MNVKIKYNLLEYCEYLGGIFGVYKNYLNMDIKDPNLKIRFFDFL
                     EELLSDGVIELCDYRENPPKILTGSPKTQVDELRRIWPDMEEMLLYFPDNPWFYVEHF
                     WWGATCPIELTQLPKIEIYEEQMKQGK"
     gene            complement(4018..4176)
                     /locus_tag="WJL87_000717"
     CDS             complement(4018..4176)
                     /locus_tag="WJL87_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799179.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNETELARMGLRL
                     KYNGQLTD"
     gene            complement(4184..5338)
                     /gene="lpxB"
                     /locus_tag="WJL87_000718"
     CDS             complement(4184..5338)
                     /gene="lpxB"
                     /locus_tag="WJL87_000718"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ7799180.1"
                     /translation="MVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARFVGIGGE
                     LMKAEGFESLYDQERLAVRGFAEVVRRLPEILRIRRGLVRDLLSLKPDVFVGIDAPDF
                     NLGVAERLKRSGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLYLDAGGR
                     AEFVGHPMAQLMPLEDDRETARKTLGVDAGIPVFALLPGSRVSEIDYMAPVFFQTALL
                     LLERYPAARFLLPAATEATKRRLAEVLQRPEFAGLPLTVIDRQSETVCRAADAVLVTS
                     GTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPELLQSEAK
                     PEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(5404..6396)
                     /locus_tag="WJL87_000719"
     CDS             complement(5404..6396)
                     /locus_tag="WJL87_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ7799181.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPLDLAQFAVMLENGTKM"
     gene            6889..9648
                     /locus_tag="WJL87_000720"
     CDS             6889..9648
                     /locus_tag="WJL87_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ7799182.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFRDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAELDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVHDQVAEFMSYVMPGNVGRLKLYQDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGSDIEDTAFKTNMEAAKEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRMVQEEAMKDNTGEVHAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRIDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGSEKAKASRPEPAVKGVRHTQPAPTVAPEKKASWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAGRADEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRSE
                     RGQTAPETATVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEVATVPAEEMIQV
                     ETRQG"
     gene            complement(10273..11262)
                     /gene="pdxA"
                     /locus_tag="WJL87_000721"
     CDS             complement(10273..11262)
                     /gene="pdxA"
                     /locus_tag="WJL87_000721"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ7799183.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKNLLRA
                     RAEALGKSVVLRDFDPESGGAAYGELEVLHIPAAEAVEAGRLNPANAAYVLQLLDTAL
                     AGISDGIFDGIVTAPLNKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLRV
                     ALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGHL
                     GHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKYH
                     SFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            11422..12849
                     /locus_tag="WJL87_000722"
     CDS             11422..12849
                     /locus_tag="WJL87_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ7799184.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            13174..15069
                     /gene="mnmG"
                     /locus_tag="WJL87_000723"
     CDS             13174..15069
                     /gene="mnmG"
                     /locus_tag="WJL87_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ7799185.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGERISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTYEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIEGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAVEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            15138..15722
                     /gene="rnhB"
                     /locus_tag="WJL87_000724"
     CDS             15138..15722
                     /gene="rnhB"
                     /locus_tag="WJL87_000724"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ7799186.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAAAWHVAAATPEEIASLNILHATMLAMKRAVDGLAVRPERIFIDG
                     NRIPEHLGIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQTLF"
     gene            15735..15980
                     /locus_tag="WJL87_000725"
     CDS             15735..15980
                     /locus_tag="WJL87_000725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799187.1"
                     /translation="MPSELQTAFPASQLQRERTHTAIPAKNRKSKKKPKIRHSRAGGN
                     PIRPVSVFFEFQVTSKPPFPQKRESRNSKLQEFIKND"
BASE COUNT         4165 a         4878 c         3998 g         3444 t
ORIGIN      
        1 accatatttg aacctgaccg aagcataagc cgccatcaaa gccccttgca cgccacttcg
       61 cgccgcccga tttcctcttt caacggcacg gcgttcaaca tccgcagacg ttcggcaaaa
      121 tccgcaaacg gcagcagctc cgaaccgaaa cggcgcatat gccccgtatc ctgctggcag
      181 tctatcagtt ccacacccaa agacgcgaga aacggcacgg cgcaggcaaa cgcgattttc
      241 gacgcatccg gttgtaatgc gaacatcgat tcgccgtaaa acaccctgcc gatctgaacg
      301 ccgtaaaacc cgcccgccaa cctcgtttta ccgttttcat cgggataatg gcactcaaac
      361 gaatgcgcgt atcccatttc gtgcagcttc aaatatgccg tctgaaactc gggcgcaatc
      421 caagttccgt cctgattcgg gcgcaccgct gccgcacaat gcgcgaccac ttccgcaaaa
      481 cagccgttga ccgtaacccg atagctgcag ttgcgtagcg ttttctccag cgagcgcgga
      541 acgtgcagac gttcgggaac aatcaccgca cgcggcccga ccgcatacca aaaaaaccaa
      601 ccgtctcgca aaaaccaagg aaacacgccg ttccgatacg cctcgagcag ccgccccgcg
      661 tccaaatccc tgctcacgcc gaccagcccg tcgcaccgtg ccaaagcata ggcaggatcg
      721 ggaaaggcat aattgtcagg ggcaagcagc ggaatacgca tggcagttct ccaaaaacac
      781 agacggcatc caaaatcatg aatgccgtct gaacaacaaa ccgtccggat ttaacgtttg
      841 cccttggcct gacagtcgct gcacacgccg tacatataaa gcgcgtgatc gacgatgcgg
      901 tagccgtttt cctcggcgat tttgtcttgc agggcttcga tttcgggatt gtggaattcc
      961 gttacctcgc cgcacttcac acagacgata tggtcgtggt ggtcgccctt gtccaactca
     1021 taaaccgcct tgcccgtttc aaaatggtgg cgttgcaaaa tgcccgcctg ctcaaattgc
     1081 gtcagcacgc gataaatcgt cgccacaccg atttccacac cctcttccaa caaaatacgg
     1141 tacacatcct cggcactcaa atgctcttcc gcatgagtct cgaacaaatc caaaatcttc
     1201 aaacgcgggc cggtaacttt cagtccactg tctttcagtt gcgcaatgtt gctgaatttt
     1261 tccataatat tcaatatccc tgtaaaataa tagacgctat aatacgcaat ttcagcctgc
     1321 ttgcccacta tcacaccata gcagtttgca atagcaaaac cgcaacggcg gacgggcagt
     1381 cggacacaga cagggtgaaa taccgcttaa gcgtatgatt atcgttcgct tttataaaat
     1441 attcaagcag tgctacacta gacatcccga tttgcacaga aaggttctcc cgtgaacaaa
     1501 accctcatac tcgtcctttc cgccctgttc agcctgaccg cgtgcagcgt cgaacgcgtt
     1561 tcactgttcc cctcgtacaa actcaaaatc atacagggca acgaactcga accccgcgcc
     1621 gttgccgccc tgcgccccgg catgaccaaa gaccaagtcc tgctcctgct cggcagcccc
     1681 atgctgcgcg acgcattcca taccgaccgc tgggactata ccttcaacac ctcccgcaac
     1741 ggcatcatca aagaacgcag caatctgacc gtctattttg aaaacggcgt gctcgtacgc
     1801 gccgaaggcg acgccctcca aaatgccgcc gaagccctcc gcgcgaaaca aaacgcagac
     1861 aaacaataag gaacacacat gataccgctc aaaatcgcca tcgccggcgt caacggccgc
     1921 atggggcgcg tattggttga agccgtcaac aaccatcccg acaccgtcct ttccggcgcg
     1981 ctcgaacact cgggctcaga agccctcggg ctggacgcag gctacgccgt cggactcaaa
     2041 accggcatcg ccatttcaga cgacgttgac accgttctcg cacaaagcga cgtactcatc
     2101 gactttaccc gccccgagcc gaccctcaaa cacctgcaaa aatgcgttga aaaacaagtc
     2161 aacatcatca tcggcacaac aggcttcgac gatgcgggca aagccgccat ccaagccgca
     2221 ggcgaaaaaa caggcgttgt cttcgccgcc aacttcagcg tcggcgtcaa cctcaccttc
     2281 cacctcctcg acaccgtcgc ccgcgtatta aacgaaggct acgacatcga aatcattgaa
     2341 ggtcaccacc gccacaaagt cgatgccccc agcggcaccg cgttacgcat gggtgaagtc
     2401 atcgccggcg cgctcggccg cgacctcaaa caatgcgccg tttacggccg tgaaggccac
     2461 accggtccgc gcgatccgtc caccatcggc tttgccaccg tccgcgcagg cgacatcgtc
     2521 ggcgaccaca ccgccctctt cgccaccgac ggcgaacgcg tggaaatcac ccacaaagcc
     2581 agcagccgca tgacctttgc cgccggtgcc gtccgcgccg cagtttgggt aaacggcaaa
     2641 acgggtttgt acgatatgca ggatgtactc gggctgaaca accgttaacc cccatacaaa
     2701 atgccgtctg aaaagatatt gttcagacgg cattttgccg acaggctccg tatcggcata
     2761 tcaatgtttc aacacacagg acgacacata aagcgtcgcc ctatgtgttg ccctgattcg
     2821 gaaggggtta cgcccctccc aaataaagtc tgattctacc gccccgaagg acagatgtcc
     2881 gagtggcggg gtttcaaccg aaaaggaaat acgataaagt tgcctgctca gcataacaag
     2941 ctacggctcg tttaattgaa actctcctga tctaaaaatt ctaactctat ttcccggcaa
     3001 actatatcta tactaaaacc ggtcggaaga tcatacctac tgcctatcac ttccagcgca
     3061 cctccataat ccaagatttc atcatcctgc tttaattctc caagttccaa tacatttttg
     3121 atatttttat cttggttctt cgtggcgtat ccatagataa atttcagata gctgaactgc
     3181 ttgaatttaa tttttaccca gtctgtataa tttggtgtag gaatctcttg aggaaagtcc
     3241 ggataatcca cattctggac gatgatatgg aattcatcgt ttttcaaagt atagtttaat
     3301 agagtgccct catatatata cagaaattga taatcttttt ttggaatggt ataagttgtc
     3361 atattatttt accttttcaa aaacaatgag tttaagattt tttgagtcca agttggttct
     3421 actactctta tgaaatttca gttgtttaaa ctcttccttt attttaagga atttttcaac
     3481 tagccgagaa caaactggag caaaatggac gattgagttt aggaatcaac ctgattcagt
     3541 acatgggcaa agattagaat taaaatttag ataaaggtta cgttatgaat gtgaagataa
     3601 aatataatct tttagaatat tgtgaatatt taggtggtat ttttggtgtt tataaaaatt
     3661 atttaaatat ggatatcaaa gatccaaatt taaaaattag attttttgat tttttggaag
     3721 aacttttatc agatggtgtt attgaattat gcgattatcg tgaaaatcct ccaaaaatat
     3781 taactggttc gccaaaaaca caggtagatg aattaagaag aatttggccg gatatggaag
     3841 agatgttgct atattttcca gataatcctt ggttttatgt agaacatttt tggtggggag
     3901 caacttgccc tattgaactg acacaattgc ctaaaataga aatttacgaa gaacaaatga
     3961 aacaaggtaa gtaagcgtat tagtataagc tataatcata aatcatatat aacaaaatta
     4021 atcagttaac tgaccattat attttaacct taatcccatt cgggccagtt ctgtttcatt
     4081 tggaattcct cttaaatact tattgtttga tgtgccatta atcacaattt ctcccccttt
     4141 ttttgtaata cgtgctgctt cgggaaaata atccattaac cgctcaacat cccgcctctt
     4201 ccaaaaccgc gcgcgcggcc aaatccgccg tatctttttt caacagcagg tgcaacgccc
     4261 tgaaatcctg ttgcagcgcg gcaaccttat cggggtgttc gtaccagtcc gccaacgccg
     4321 ccgccagttt ttccggtttt gcttcagatt gcaataattc cggcacagcc tccttaccca
     4381 acaggatatt cggcaggccg acatgcggca ctttgatttt gcgtttcaca taagcatagg
     4441 tcagcggcga aatcttgtag ctgatgacca tcggacgctt acacaacgcc acctccaaag
     4501 ttgccgtacc gctcgttacc agcaccgcat ccgccgccct gcacactgtt tcagactgtc
     4561 tgtcgattac cgtcagcggc aatccggcaa actccggccg ctgcaaaact tccgccaaac
     4621 gccgcttcgt cgcctccgtt gcggcaggca gcaggaagcg tgcggcggga tagcgttcca
     4681 acaacaataa tgccgtctga aaaaacaccg gcgccatata gtcgatttcg ctgacgcggc
     4741 tgccgggcag cagggcgaat acggggatgc cggcatccac gcccaaagtt ttccgcgccg
     4801 tttcacggtc gtcttccaag ggcataagct gcgccatcgg atgaccgaca aactccgcac
     4861 gtccgcccgc atcaagataa agctgcggct ccatcgggaa caggcacagc acgcggttga
     4921 cctgatgcac gattttgccc acgcgttccc gccgccacgc ccaaaccgac ggactgacat
     4981 aatgcacggt cggaatcccc gaccgtttga gccgctccgc cacacccaga ttaaaatcgg
     5041 gcgcatcgat gccgacaaag acatcaggtt tcaacgacag caaatcccgt accagccccc
     5101 tgcgtatccg taaaatttcc ggcagccgcc tgaccacttc ggcaaagccg cgcaccgcca
     5161 gtcgctcctg atcataaagg ctctcgaaac cttccgcctt catcagttcg ccgccgatac
     5221 cgacaaaacg cgcctgcggg caacgcttgc ggatggcgcg tatcaggtgc gcccccaaca
     5281 ggtcgcccga cgcttcgccg acactgacag caatcaaagg gcttttttta tcaaccatat
     5341 tcgttcttct tcacatactt tcacgtcccg atgccgtctg aaggcttcag acggcatcgg
     5401 ggttcacatt ttcgtcccgt tttccaacat caccgcaaac tgtgccaaat ccagcggcag
     5461 ctccgccttc aacaccagcg gctcgcccgt gagcggatgg ttcaagtgca gctcggacgc
     5521 gtgcaaaaac atccgcttca aacccaactt ctgcaaacga cggttcgcct gataatcgcc
     5581 gtagcgttcg tcgcccgcaa tcggacagcc ttgagattgc aggtggacgc ggatttggtg
     5641 cgtgcgcccc gttttcaacg tcgcccgcac caaagtcagg tgcgacagcc cgacaccgtg
     5701 caaaatgccg tctgaaaaac ggcttaacac acggaacacc gtatgcgccg actgcccgtc
     5761 cgcactgacg cgcaccatct tttcgccttg tgcgccggta tatttgaaca ggggcagttt
     5821 gacatggaaa ttgtcgtccg gcagtttgcc cacccccagc gcaaggtaga tttttttggg
     5881 gtggtcgtta cggatggctt cgtgaagttt gacgagcgcg ctgcgtttct tcgccaccat
     5941 caacaagccg ctcgtatcct tgtccaaacg atgaaccaac tccaaatact tcgcctccgg
     6001 acgggcgcgg cgcaactgtt cgataacgcc gaaactcacg ccgctgccgc cgtggacggc
     6061 aacgccggac ggtttgtcga tgaccaaaag cgcatcgtct tcgtaaacaa cgtcaaacgc
     6121 acgcgccggt acggcggcac gcctttcaga cggcatttcc ttctccgcca cgcgcacagg
     6181 cggaatccgc accgtatccc cctccgcaat acggctgtcg ggtttgcagc gtttcttgtt
     6241 caaccgcacc tcgccggcgc ggataatgcg gtggatatgg ctcttgggca cgcccttgag
     6301 gatttttatc agatagttat caaggcgttg ccccgcctca tgttcggcga cccctatcaa
     6361 gctgaccgaa tctttgctta tttcgtgcgt tttcatctat aatccgaaca tccgtttcag
     6421 caaaaagcgc gcccgccgcg cctcctgaaa cggtttactt taaaacggaa ttttatatta
     6481 aaaacgcact ccgcaaagca ttttccctgc gcctgttccc agccggcagg cgcagaacgt
     6541 agtcaagttt gaattgattt tgccgtttcg gcgcggaagt aagacggcag ccgggcccaa
     6601 gccccaaacg caaaccgccc gaaaacacag gcatcacgat aacaagaaga agccggcacc
     6661 catttttccg accataccgc tcggaacgcg gcagacagac cgccgcatcg atctttatag
     6721 ccttctgtat ccgacccttc cgcacacggt tctccggaac gtgcatccct gcggattttc
     6781 aactcaaaaa ctgattaccg gttttatccg aagacatcgg aaaacccagc cttacggaat
     6841 gccgaaccgg accggcgcgc ccgaatgccg cccgtccacg aggtgatcat gaaaagaatg
     6901 ttattcaacg caacgcaggc cgaagagctg cgcgttgcca tcgtcgatgg ccaaaacctt
     6961 ttggacttgg acatcgaaac gctgggcaaa gaacagcgta aaggcaatat ctacaaaggt
     7021 atcattaccc gcatcgagcc gtcgctggaa gcatgtttcg tcgattacgg aaccgaccgc
     7081 cacggctttt tgccgtttaa ggaagtatcg cgttcatatt tccgcgacta cgaaggcggc
     7141 agggcgcgta ttcaggacgt gctcaaagag ggcatggaag tcattgtcca agtcgaaaaa
     7201 gacgaacgcg gcaacaaagg cgcggcgctg accactttca tcagcctggc cggccgctat
     7261 ttggtattga tgccgaacaa cccgcgcggc ggcggcgtat cccgccgtat cgaaggcgaa
     7321 gagcgtcaag agttgaaagc cgccatggcc gaactcgaca ttccaaacgg catgagcatc
     7381 attgcccgta ctgccggcat cggccgcagt gcggaagagt tggaatggga cttgaactac
     7441 ctcaaacaac tctggcaagc cattgaagaa gcaggcaaag cacaccatga cccttacctg
     7501 ctctttatgg aaagctcact gctgatccgt gcgattcgag actatttccg tcccgatatc
     7561 ggtgagattt tggtggacaa tcaagaagtt cacgaccaag ttgccgagtt catgagctac
     7621 gtcatgccgg gcaatgtagg ccgtctgaaa ctctatcaag accacacgcc gctgttctcc
     7681 cgtttccaaa tcgaacacca aattgaaagc gcgttctcac gcagcgtcag tctgccttcc
     7741 ggcggcgcga ttgtgatcga ccacaccgaa gccctggttt ccatcgacgt caactctgcc
     7801 cgcgccacac gcggctctga cattgaagac accgcgttca aaaccaatat ggaagccgcc
     7861 aaagaggttg cccgtcaaat gcgcctgcgc gacttgggtg gtttggtcgt catcgacttc
     7921 atcgacatgg aaaatccgaa acatcagcgc gatgttgaaa acgtcctgcg cgatgcgctc
     7981 aaaaaagacc gcgcccgcgt gcaaatgggc aaactttcgc gtttcggcct tttggaattg
     8041 agccgccaac gtctgaaacc ggctttgggc gaaagcagcc acgtcgcctg tccgcgctgc
     8101 gccggcacag gcgtcatccg cggcatcgaa tccaccgccc tgcacgtttt gcgtatggtt
     8161 caggaagaag cgatgaagga caacaccggc gaagtgcatg cacaagtgcc cgtcgatgtc
     8221 gccaccttcc tgctgaacga aaaacgcgcc gagctgtttg cgatggaaga gcgtttggat
     8281 gtcaacgtcg tcctgattcc aaacatccac ttggaaaatc cgcactacga aatcaaccgc
     8341 atccgcattg atgacgtaga agaagacggc gaaccgagct acaaacgcgt tgccgagccg
     8401 gaagaagacg aatccgccaa acctttcggc agcgaaaaag ccaaagcttc ccgtcctgag
     8461 ccagctgtca aaggcgtacg ccatacccag cctgcaccga ctgtcgcccc tgagaaaaaa
     8521 gcctcttggt gggacagctt caaagcttgg ttgaaacgca ttttcggcgg cagcgaaacc
     8581 caagccgcgc ccgctgccga aacctccgaa aaacgcagca cggcaaaccg cagcggcagc
     8641 cgcgccaaca accgccgcca aaacccgcgc cgcagcaaac gcgaaggcag caaagtagaa
     8701 gtccgcgaag tggcaggcaa aactgccgga caggaagcgc gcgccgacaa agccgaaacg
     8761 cgcaacaacg gcaaccgccg ccgcaacgaa cgcggcgacc gtgccgccgg acgcgccgac
     8821 gaagcggaaa tccaagaccg caacatacag cctgccgcaa ccgttgcaga tgccgcaccg
     8881 tccgaaaccg aagtgcaaac cggaaaacgc cgccgcaacg gcagccgcag cgaacgcggc
     8941 caaaccgcgc cggaaaccgc caccgttgcc gaaacaaccg ttcagacagc ggaaaacacg
     9001 ccgtccgaac cgcataccgc agaagacaaa ggcagcaagc ccaaatccga acgcaaccgc
     9061 cgcgaacgcg acagccgaga tgccaaagaa cgccgcgagc gcaacaatca gcgcgaccgc
     9121 cgtcaaaacg gcaaaaaacg caatattccg tctgccgcca aaatcgagca gtacctgaat
     9181 attcacgaca ccgccgacaa agtccgttcc gccgccgcgc acgttttcgg cgaaaccgac
     9241 gcaaacgcgc cgattaccgt cagcattgcc gatccggttg cagaaaggga tcttccgaca
     9301 gcatctcccg ccgtttcaaa cggcgacgca ccggtttatg atgcggcgga aaaaatccgc
     9361 cgtgccaccg ccgccatcct gcccgaaggc gcgacaccga aagccgaagc acaggaaatg
     9421 ccgtctgaaa ccgcaacctt tacggctgcg gcggaacagg cacgggaaac cgcacaaacc
     9481 ggcggactcg tcctgatcga aaccgaccct gccgcattga aggcatgggc ggcacaaccc
     9541 gaagtccaag ccggacgcgg tttgcgccgt tccgaacagc ccaagccgtc tgaagtcgca
     9601 accgtccctg ccgaagaaat gatccaagtc gaaacccggc aaggctgaac cgacggcggc
     9661 aaaaagaggt tctgttccgc agaacctctt ttttacatgg gttcggatac ctgcaatgcc
     9721 gtctgaaact tcgccattcc cgtgattacc gaaacattcc tccattccca tgattcccgc
     9781 aacattccgt cattcccatg attcccgcaa cattccgtca ttcccgtgaa aacgggaatc
     9841 tagaacctca aactttcaga taatctttga atattgccgc cgcccaaagg tctggattcc
     9901 cacaacattc cgtcattccc gtgaaaacgg gaatctagaa cctctaaact ttcagataat
     9961 ctttgaatat tgccgtcgcc caaaggcctg gattcccgcc tgcgcgggaa tgacggttta
    10021 gaagttgccc gaaacctcaa aaaaaaccga aaccgaacaa gccggattcc cgcaaccttt
    10081 cgtcattccc gtgaaaacgg gaatctagaa cctcaaactt tcagataatc tttgaatatt
    10141 gccgtcgccc gaaggtctgg attcccgcct gcgcgggaat gacgatttgg aaattacccg
    10201 aaacccaaaa acaactgaaa ccgaacagac cggattcccg cctgcgcgga aatgacgggt
    10261 cttttatcat ctttaaaggc tgccgcgcgc catctcgacg gcggtctcca cggcagttat
    10321 caggctgccg gaatccgccc tgccggttgc cgccaaatca agcgcggtgc cgtgatcgac
    10381 ggaggtgcgg ataaagggca ggccgagcgt gatgttcacg ccctgtccga agctgtggta
    10441 tttcaacacg ggcagccctt ggtcgtggta catcgccaat acggcatccg caccttcgag
    10501 cataaacggt tggaacaagg tatccgccgg atacgggccg gcgaggttta tcccttcgcg
    10561 gcgcaggttt tcgagcgcgg ggataatggt gtcggtttct tcgtgtccga ggtgtccgcc
    10621 ttcgccggcg tgggggttga gtccggcgac taggattttg ggatttttga tgccgaattt
    10681 gtgttttaag tcgtgatgca aaatgcgtgc gacgctttca atcagcggtt gcgtgatggc
    10741 ggcggcaacg tctttcagcg gcaggtgggt cgttacgagg gcgacgcgca ggcctttgcc
    10801 ggcaagcatc atcacaacct gccccgtgcc gcttttttcc gccagatatt cggtgtgtcc
    10861 gctgaaaaaa cctgtgcttg cgcgcgcgtc gttgatgatg cctttgttca gcggcgcggt
    10921 aacgatgccg tcgaaaatgc cgtctgaaat gcctgcgagc gcggtatcca aaagttgcag
    10981 cacataggcg gcgttggcgg gattgagccg ccctgcctca accgcttcgg cggcagggat
    11041 gtgcagcact tccagctcgc cgtatgccgc gccgcctgat tccggatcga agtcgcgcaa
    11101 gacgacgctt ttgcccaagg cttcggcgcg cgcgcgcaat aggtttttgt cgcccaatac
    11161 cgcgcagcgg cagggcaggc gtgcaaacgc caagtccaaa caaatatcgg ggccgatgcc
    11221 ggcaggctcg ccggaagtaa cggcaaaaac aggctgtttc atcgtgtttg ctccaaacaa
    11281 aatgcgattc taacgccgca gccgcgcggc gatgtaaatt tttctgattt tgttgacaat
    11341 ctgctagaat gggcgtttac aaaatttaaa ccctgcttgc ataccgccaa tatgtgcggg
    11401 tttcaacttt aaggaagcga tatgaacgag aactttaccg aatggctgca cggctgggtc
    11461 ggcgccatca acgatccgat gtggtcatac ttggtttata tgcttttggg tacggggctt
    11521 ttcttcaccg taaccacggg ctttgtccaa ttccgcctgt tcgggcgcag catcaaagaa
    11581 atgctcggcg gccgcaaaca gggggacgac cctcacggca tcacgccgtt tcaggcattt
    11641 gtaaccggcc ttgccagccg cgtgggcgtg ggcaatatcg cgggcgtggc aatcgccatc
    11701 aaagtcggcg gaccgggcgc ggtgttttgg atgtgggtaa ccgccttaat cggtatgagt
    11761 tcggcgtttg tcgaatcttc gctggcgcag ctctttaaag tccgcgacta cgacaaccac
    11821 catttccggg gcggccctgc ctactacatc actcaagggc tggggcagaa atggctgggc
    11881 gtgttgttcg ccctgagcct gattttctgt ttcggctttg tgtttgaagc ggttcagacc
    11941 aataccatcg ccgataccgt caaagcggca tggggttggg agcctcatta tgtcggcgtc
    12001 gccctggtga ttttaaccgc gccgattatc ttcggcggca tcaggcgcat atctaaagcg
    12061 gcggaaatcg tcgtccccct gatggcggtt ttgtacctct ttatcgcgct tttcatcatt
    12121 ttgaccaata ttccgatgat tccagacgtg ttcggtcaga ttttttcggg cgcgttcaaa
    12181 ttcgacgcgg cagcaggcgg cttactcggc ggtctgattt cacaaacgat gatgatgggc
    12241 atcaaacgcg gcctgtattc caacgaggcg ggtatgggtt ccgcgccgaa cgccgctgcc
    12301 gccgccgaag tgaaacaccc tgtttcgcaa ggtatgattc aaatgctggg cgtgtttgtc
    12361 gataccatca tcgtttgttc ttgcaccgcc ttcatcatct tgatttacca acagccttac
    12421 ggcgatttga gcggtgcggc gttgacgcag gcggcgattg tcagccaagt ggggcaatgg
    12481 ggcgcgggct tcctcgccgt catcctgttt atgtttgcct tttccaccgt tatcggcaac
    12541 tatgcctatg ccgagtccaa cgtccaattc atcaaaagcc attggctgat taccgccgtt
    12601 ttccgtatgc tggttttggc gtgggtctat ttcggcgcgg ttgccaatgt gcctttggtc
    12661 tgggatatgg cggatatggc gatgggcatt atggcgtgga tcaaccttgt cgccatcctg
    12721 ctgctctcgc cactggcgtt tatgctgctg cgcgattaca ccgccaagct gaaaatgggc
    12781 aaagaccccg agttcaaact ttccgaacat ccgggcctga aacgccgtat caaatccgac
    12841 gtttggtaaa tcccgccctt accggagccg cttccccgcg aagcggcttt tccctttccg
    12901 cacactgtaa aaacagggcg aacaagcgta caatcccaac cctttacttt tgaatccatt
    12961 tcgtttttca gacggcatat tgaatatagt ggtttaacaa aaatcaggac aaggcgacga
    13021 agccgcagac agtacaagca gtacgaaacc gattcacttg gtacttcagc accttagaga
    13081 atcgttctct ttgagctaag gcgaggcaac gacgtactgg tttttgttaa tccactataa
    13141 atgccgtctg aaacaccgtc aggcaataca cacatgaccc acatgattta ccccaaaacc
    13201 tacgacgtta tcgtcgtcgg cggcggacac gccggcacag aagccgcgct cgccgccgcc
    13261 cgtatgggcg cgcagacgct tttgctcaca cacaatattg aaacgctcgg acaaatgtcg
    13321 tgcaaccctt ccatcggcgg catcggcaaa gggcatttgg tacgcgaact cgacgcgctc
    13381 ggcggcgcaa tggcgttggc caccgacaaa tccggtatcc agttccgccg cctgaacgcc
    13441 agcaaaggcg cggcagttcg agccacgcgc gcgcaggccg accgcatttt gtacaaagcc
    13501 gccatccgcg aaatgttgga aaaccaagaa aacctcgacc ttttccaaca agccgtcgaa
    13561 gacgtaacgc tcgacggcga acgcatcagc ggcgtaatca ccgcgatggg cgtggaattc
    13621 aaagcccgcg ccgtggtgct gaccgcaggc acatttttgt ccggcaaaat ccatatcggt
    13681 ttggaaaact acgaaggcgg ccgcgccggc gaccccgccg ccaaatcgct gggcggacgt
    13741 ttgcgcgaat tgaagctgcc gcaaggccgt ctgaaaaccg gcacgccgcc gcgcatcgac
    13801 ggacgcacga ttgacttttc ccaactgacc gaacagcccg gcgacacacc cgttcccgtc
    13861 atgtccgtgc gcggcaacgc cgaaatgcac ccgcgccaag tgtcctgctg gattacgcat
    13921 accaacacgc aaacccacga catcatccgc tcaggcttcg accgcagccc gatgtttacc
    13981 ggcaaaatcg aaggcgtggg cccgcgttat tgtccgtcta tcgaagacaa aatcaaccgt
    14041 ttcgccgata aagacagcca ccagattttc ctagaacccg aaggcctgac tacgtacgaa
    14101 tactatccta acggtatctc caccagcctg ccgttcgaca tccaaatcgc gctcgtccgc
    14161 agtatgaaag gtttggaaaa cgcccatatc ctgcgccccg gctacgccat cgaatacgac
    14221 tacttcgacc cgcgcaacct caaagccagc ctcgaaacca aaaccattga gggactgttt
    14281 ttcgctgggc aaatcaacgg tacgaccggc tacgaagaag ccgccgcgca aggtttattg
    14341 gcaggcgcga acgccgtgca atatgtgcgc ggacaagacc cgctcctgct gcgccgcgaa
    14401 caagcctacc tcggcgtatt ggtggacgac ctcatcacca aaggcgtgaa cgaaccctac
    14461 cgaatgttca ccagccgcgc cgaataccgc ctgcaactca gggaagacaa cgccgacatg
    14521 cgcctgaccg aagacggcta caaaatcggc ttggtgggtg aagcgcaatg gcgtatgttc
    14581 aacgaaaaac gcgaagccgt cgaacgcgaa atccaacgtt tgaaaacaac gtggtacaca
    14641 ccgcaaaaac tcgccgaaga cgaacaaatc cgcgtattcg gacaaaaact cagccgagaa
    14701 gccaacctgc acgatttgtt acgccgcccg aacctcgact acgccgcgct gatgacgctc
    14761 gaaggcgcga tgccgtctga aaacctctcc gccgaagtca tcgaacaagt cgaaatccaa
    14821 gtcaaatacc aaggctatat cgaccgccaa aacgaagaaa tcgacagccg ccgcgacatc
    14881 gaaaccttaa aactgcccga cggcatcgat tacggcaaag tcaaaggctt gtcggcagaa
    14941 gtgcagcaaa agctcaacca gcacaaaccc gaaaccgtcg ggcaggcaag ccgcatttcc
    15001 ggtgtaacgc ctgccgccgt cgcactgctg atggtgcatt tgaagcgcgg atttaaagac
    15061 gcgaaataaa cacatcggcg cgatgccgtc tgaaacaccg ttcagacggc attcccacca
    15121 tcccgacagg aaacatcatg cacatactga ccgccggcgt ggacgaggca ggacgcggac
    15181 ctttagtcgg cagcgtgttt gccgccgccg tcatccttcc ggaaacattc gacctgcccg
    15241 gactgaccga ctccaaaaaa ctcagcgaga aaaaacgcga cgcgcttgcc gaaatgataa
    15301 aaaatcaggc ggccgcgtgg cacgtcgccg ccgccacgcc cgaagaaatc gccagcctca
    15361 acatcctgca cgctaccatg ctcgccatga aacgcgccgt tgacggtttg gctgtgcgtc
    15421 ccgaaagaat attcatcgac ggcaaccgca ttccagaaca tttaggtatc cctgccgaag
    15481 ccgtcgtcaa aggcgacagc aaaatcatcg aaatctccgc cgcatccgtt ttggcaaaga
    15541 ccgcacgcga tgcggaaatg tacgcactgg cgcaacgcca tccccaatac ggtttcgaca
    15601 aacacaaagg ttacggcacg aagcagcatc tggaagccct caaacaatac ggcgtgctgc
    15661 ccgaacatcg ccgcgacttc gcccccgtca gaaacctgct cgcgcagcag acgctgtttt
    15721 aaaccggcac aaaaatgccg tctgaacttc agacggcatt tccagcatcg caacttcaaa
    15781 gagaaagaac ccataccgcc attcccgcga aaaacagaaa atcaaaaaag aaacctaaaa
    15841 tccgtcattc ccgcgcaggc gggaatccaa tccgtccggt ttccgttttt tttgaatttc
    15901 aggtaacttc caaaccgcca ttcccgcaaa agcgggaatc tagaaactca aagctgcaag
    15961 aatttatcaa aaatgactga agctcaaaaa accggattcc tacgaaaaca ggaatccgga
    16021 gtctcagggc tggcaaaacc gttttacccg ataagtttcc gtaccgacag acctagattc
    16081 ccgccttcgc gggaatgacg aaattttagg cttctgtttt gattttttgt ttttgcggga
    16141 atgacgaaat tttagattgc aggcatttat cggataaaac agaaattaag cgtgacgaaa
    16201 atttatccga aatcacagca acttttccgc gtcattcccg caaaagcggg aatctagaaa
    16261 ctcaaagctg caagaattta tcaaaaatga ctgaaactca aaaaaccgga ttcccgcgaa
    16321 aacaggaatc cggagtctca gggttggaaa aaccgttttt cccgataagt ttccgtaccg
    16381 acagacctag attcccgcct gcacgggaat gacgatattt cagttttgtg tttttgattt
    16441 ttttgttttt gcgggaatga cgatatagtg gattaacaaa aatca
//
LOCUS       DAWKST010000033        15896 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.033, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000033 DAWKST010000000
VERSION     DAWKST010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15896
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.033"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(206..277)
                     /locus_tag="WJL87_000726"
     tRNA            complement(206..277)
                     /locus_tag="WJL87_000726"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(242..244),aa:Arg,seq:cct)
     gene            complement(297..743)
                     /locus_tag="WJL87_000727"
     CDS             complement(297..743)
                     /locus_tag="WJL87_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEZ7799188.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(748..1722)
                     /gene="ispB"
                     /locus_tag="WJL87_000728"
     CDS             complement(748..1722)
                     /gene="ispB"
                     /locus_tag="WJL87_000728"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ7799189.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDGKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVDSDSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESLVRVS"
     gene            complement(1738..1926)
                     /locus_tag="WJL87_000729"
     CDS             complement(1738..1926)
                     /locus_tag="WJL87_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799190.1"
                     /translation="MVNIRTPLCGTRHCIVICLFCQSLRGSDNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLMEA"
     gene            1946..2254
                     /gene="rplU"
                     /locus_tag="WJL87_000730"
     CDS             1946..2254
                     /gene="rplU"
                     /locus_tag="WJL87_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ7799191.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2279..2551
                     /gene="rpmA"
                     /locus_tag="WJL87_000731"
     CDS             2279..2551
                     /gene="rpmA"
                     /locus_tag="WJL87_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ7799192.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            2786..3970
                     /gene="ubiM"
                     /locus_tag="WJL87_000732"
     CDS             2786..3970
                     /gene="ubiM"
                     /locus_tag="WJL87_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ7799193.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNVSILTDTVVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGASSL
                     LEKYSNKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(4241..4396)
                     /gene="rpmG"
                     /locus_tag="WJL87_000733"
     CDS             complement(4241..4396)
                     /gene="rpmG"
                     /locus_tag="WJL87_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ7799194.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(4428..4661)
                     /gene="rpmB"
                     /locus_tag="WJL87_000734"
     CDS             complement(4428..4661)
                     /gene="rpmB"
                     /locus_tag="WJL87_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ7799195.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(4911..6434)
                     /gene="farB"
                     /locus_tag="WJL87_000735"
     CDS             complement(4911..6434)
                     /gene="farB"
                     /locus_tag="WJL87_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ7799196.1"
                     /translation="MDYPPLKGAALAWITLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     ALVFFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKYRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVTALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPIIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGAGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSFVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     QGISDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGH"
     gene            complement(6458..7630)
                     /gene="farA"
                     /locus_tag="WJL87_000736"
     CDS             complement(6458..7630)
                     /gene="farA"
                     /locus_tag="WJL87_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ7799197.1"
                     /translation="MATHGDEVKIQNRQEADSAKAKRKRRLTALTLLFALAAAAAGSA
                     FFLWWQHEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVL
                     AYERAKNELIQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSA
                     EELAHARAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQ
                     RTQIRAPADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPA
                     ELVSDLYGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDV
                     DRHPLRIGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
     regulatory      7859..7903
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            7907..8380
                     /gene="queF"
                     /locus_tag="WJL87_000737"
     CDS             7907..8380
                     /gene="queF"
                     /locus_tag="WJL87_000737"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ7799198.1"
                     /translation="MSRNTEELQGISLLGNQKTRYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFETLARKRLFEHDSQ"
     gene            8455..9141
                     /locus_tag="WJL87_000738"
     CDS             8455..9141
                     /locus_tag="WJL87_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ7799199.1"
                     /translation="MYAFTAAQQQKALFWLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
     gene            complement(9644..10936)
                     /locus_tag="WJL87_000739"
     CDS             complement(9644..10936)
                     /locus_tag="WJL87_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ7799200.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLIYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            11014..11982
                     /locus_tag="WJL87_000740"
     CDS             11014..11982
                     /locus_tag="WJL87_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799201.1"
                     /translation="MKLTLAIPSLNLDKDETRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSETVALATPVWQKMGMHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDSLHGSSQGGTLFADTVWSRFY
                     PDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYASVLQQWEEHWFAP
                     IYEAVRTGKIKRLDITTDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            complement(12183..13649)
                     /locus_tag="WJL87_000741"
     CDS             complement(12183..13649)
                     /locus_tag="WJL87_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ7799202.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKSPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFDNRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(13891..15896)
                     /locus_tag="WJL87_000742"
                     /pseudo
     CDS             complement(13891..15896)
                     /locus_tag="WJL87_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         3834 a         4587 c         3806 g         3669 t
ORIGIN      
        1 ccactatatc ataaaacaac agatttccaa gccggaacat cttttacgaa gcctgaaaat
       61 catttcatga ttctaccgtc ctaaaggtcg gtttttcaag caggaagaaa aagttttcag
      121 atggcaaaaa agccctccaa cactgaaagg ccttatatcg gaaacttccc gcaacacggg
      181 aaacagacaa atgcaaatcg tcaaacctcg ccaacaggaa tcgaacctgt attttacgct
      241 taggaggcat acgttctatc cgttgaacta tggcgagccg aaatgaaaag gagattttaa
      301 ccctttccga cgacaaaaga caagatgccg gccgcaatca gcggcccgac agggataccg
      361 cccataaatg ccaccccgat aaccgtcccg attaacagcc ctgtaattaa aacaggctgc
      421 tgtcccatca aaggcacgcc gcgtcccgcc agccaagcca cgaaaatacc gataaaaacg
      481 gcggatatca ttttaaaatt caaaaattcg gcaacgggag gaacctgcgc ctttcctgaa
      541 accaacggac tcaaaacccc tatggtcaaa agaatgatac cgagattcaa cccatgcttc
      601 tcgaccaacg ggacaaactg taccaatgcc gtctgctgca tcagcagcaa tatggttgcc
      661 gagatggtaa tcgaattgtt gttgctgacc acccccaaca gaatcagcgt aaccagaaac
      721 aggggaacaa aactgaaatt catcgcctca agacaccctg accaaagatt ccttcgccag
      781 ctgaatcatg gcatccttca cttcgctgtc gggcagggca tccaaggcgg taacggcaca
      841 atcgactgct ttgcgcgcct cgcctatcga atatgccaaa gcatccgaac ggacgacata
      901 atcgtggatt ttctcaaaat agctgcgatc tgcattttcc aaagcagtac gcacatcgtt
      961 cgcaacctgt tcggaaccct gacgcatcag ataaatcaaa ggcaaagtcg gttttccttc
     1021 cgccaaatcg tcgccgacgt ttttgccggt ttcttcggtt tcgccagaat agtccagcac
     1081 atcgtcaata atttggaatg ccgtaccgac atacataccg tagtctttca acgcccgttc
     1141 gtgttcgggg gaagccttgc ccaaaattgc gccgacttga gcggcagctt caaacaattt
     1201 tgccgtttta tattggatga cttggatata ttgttcttcg gtaatgtccg tattgccgat
     1261 gttcatcagc tgcatgactt cgccctcggc aataatgttg gttgcatccg ccatcacttc
     1321 caaaacgcgc atactgtccg agtcaaccat cagttgaaag gcgcgcgtgt ataaaaagtc
     1381 gccaaccaac actgccgccg cattgccgaa cagattgttt gccgttgccc gtccacggcg
     1441 caaatcgctt tcatcgacga catcgtcgtg cagcagcgtc gaagtgtgga taaattcgac
     1501 catcgccgcc agcgaataca gtttcccgtc atcataaccg accgccttac ccgccaaaat
     1561 cgtcataatc ggacgcaggc gtttgccgcc cgcgctgatg atatatgtac cgatttgcga
     1621 aatcagtgcg acatcggatt gcaccgcacg gttgatgact tcattgactt tggcaaggtc
     1681 ttcaggcaga tgtcgctgga aatagggcag attttcgagc atggcagact ctcaggttta
     1741 cgcttccatc aaacggcaga taccgcttga aagctgattc aaatagcggg caattatatc
     1801 agcaaagcca tcagggtaca tcttttcaga cggcatatgg ttatccgaac cgcgcaaact
     1861 ttgacaaaac aggcaaataa caatacaatg ccgagttccg cacaatggtg tgcggatatt
     1921 aaccataacc cttatggagt tgagtatgta cgcggtcgta aaaaccggcg gcaaacagta
     1981 taaagtttcc gtcggcgaaa aattgaaagt agaacagata ccagccgaac tcgacagcca
     2041 aatcgaactg accgaagttt tgatgattgc tgacggcgaa tctgtaaaag ttggcgcacc
     2101 ctttatcgaa ggtgcaaaag taacggctaa agtagtggca cacggtcgtg gcgaaaaagt
     2161 ccgcatcttc aaaatgcgcc gccgcaaaca ctaccaaaaa cgccaaggcc accgccaaaa
     2221 tttcacccaa atcgaaatcg tggcaatcgc ctaaccttaa aacttaggag cattacaaat
     2281 ggcaagtaaa aaagcaggcg gcagcacccg caacggtcgc gattcagaag ccaaacgctt
     2341 gggcgttaaa gcctacggca acgagctgat tcccgcaggt tccatcatcg tacgccaacg
     2401 cggtaccaaa ttccacgcag gcgacaacgt aggcatgggc aaagatcaca ctttgttcgc
     2461 caaagttgac ggttatgttg aattcaaaac caaaggtgcg ctgaaccgta aaactgtcag
     2521 catccgtcct tacaccggtt ctgaagaata atccaaccga tacctgaagc cgcatctttt
     2581 cacgatgcgg ctttttacat accccacacc cgatttgata cacattaagc tgaaagtaaa
     2641 aatccgcata catcctcccc tgcatatttc ttcaacagcg ggttttgata taatcgccta
     2701 tctgttacag atagttcaaa cggcattcaa acccttacaa atgccgtctg aaatcatcgg
     2761 catccgtcta tgcaaaggat attttatgag tttacacagc gacatcctcg tcgtcggcgc
     2821 aggcccggcg ggtttaagtt ttgcagccga acttgccggc agcggtttga aagttaccct
     2881 gatcgaacgc agcccactga ctgttctgca aaatcctcca tatgacggac gcgaaatcgc
     2941 cctgacccat ttttcccgcg aaatcatgca gcgtttgggt atgtgggaca aaattcccga
     3001 aaacgaaatc taccctttgc gcgatgccaa agtgctgaac ggacgttccg actaccagct
     3061 ccactttccc caaccgactg aagcgcgcgg cgaacctgcc gactgtttgg gctatctgat
     3121 ttccaaccac aatatccgcc gcgccgccta tgaagtcgta tcacaactcg acaatgtcag
     3181 cattctgacc gatacggttg tcaaagaagt caaaacatct gacaacgaag cacaagtcat
     3241 tttggaaaac ggcaaaatcc tgactgcacg cctgcttttg gcggcagaca gccgcttctc
     3301 gcaaacccgt cgacaactcg gtatctcctc cgatatgcac gattacagcc gaaccatgtt
     3361 cgtctgccgt atgaaacata ccctttccaa ccaacatacc gcttacgaat gcttccatta
     3421 cggacgcacc atcgcactgc tgccgctgga agagcacctg accaataccg tcattaccgt
     3481 cgataccgat aaaatcaaca gcgttcaaaa cctttcgccc gaagaattgg cggcaagcgt
     3541 aaaagaacaa ctcaagggta ggcttggcga catggaactg gtcagcagca tccaccatta
     3601 cccccttgtc ggtatgattg ccaaacgttt ctacggcaaa cgcagcgcgc tgatcggcga
     3661 tgcggctgtc ggtatgcacc ccgttaccgc acacggtttc aacttgggtc tgtccagcgc
     3721 agatattttg gcaaaactga tactcgaagc cgagcagcgc ggtcaggaca tcggcgcatc
     3781 ttccttgttg gaaaaataca gcaacaaaca tatgctgcac gcccatccgc tttatcacgg
     3841 aaccaatatg atgctgaaac tgtttaccaa cgaaaccgcg ccggcaaaac tgctgcgcgg
     3901 tttggtattg cgtgccggca acaacttccc gccgctgaaa aagctgatta ccaaacaact
     3961 gaccggttga aaactgccga aatgccgtct gaaacgtttc agacggcatt tttgtattat
     4021 agtggattaa caaaaatcag gacaaggcga cgaagctgca gacagtacaa atagtacgga
     4081 accgattcac ttggtgcttc agcaccttag agaatcgttc tctatccttg gcagacggtt
     4141 tgcagcagat tcgcatacgg cagatgtttc aagcggaaaa gaaacacgat aaaaataaaa
     4201 aagcctccga acagtcggag gctttgcttt caaactgaaa ttatttcagt ttggtttctt
     4261 tgtacactac gtgtttacgg gcaaccggat cgaatttttt aatttccagt ttgccgggca
     4321 tagtgcgttt atttttggta gtggtgtaga agtgaccagt acctgcactg gattccagtt
     4381 tgattttatc gcgcattgca gtaatcctta attaaatagt gtttaaatta agcttcgccg
     4441 cgagcacgca aatcagccaa tacgacatca atgcctactt tgtcgatggt acgcagtgca
     4501 gcgttggaaa cgcgcaggcg aacccagcgg ttttcacttt ctacccaaaa acgacgtgat
     4561 tgcaagttgg gcaaaaaacg gcgtttggtt ttgttgttgg cgtgcgatac gttgttgccg
     4621 gacatcgggc gtttgccggt cactttgcaa actcgtgcca taatcagtct ccaaacttct
     4681 aaaatagtaa aacgcgattt ataccacgaa aaacagtgtt agttcaagtg ttttaaagaa
     4741 aaacggctgt tttgcgcgga taatccttcc gattgtcgca tctgtggaac gtttgcaggt
     4801 gcgaaacaga tggggcgcga ttatagcgga tttcgggaac tgctgcattc aaaatgccgt
     4861 ccgaacatat ttccagtatt ttcagacggc atttcaagtt ttcaaatccc tcaatgtcca
     4921 ccgccgccgt tgtggaacgg cggttttgcc agccatatga caggaatcat gatgatgaac
     4981 agcaggctgc ctgccataaa gatttcattc gatccgatga tgaagccctg ctgggtaatg
     5041 gtattgttga tgatacctag ggtttgaatg tcggaaatgc cttgttggga caaatgcgcc
     5101 gccgtttcgt gcaatgttgc ggaatagggc gtgatgtgtt cggcaaagcg tgtgtggtgc
     5161 aacgcttcgc gccgttccca cagggtactg acgaatgata cgccgacacc gcccatcagc
     5221 acgcgcaaga aattcgacag gctgccggcg gcggcaatct gtccgccctt catatgcgac
     5281 agtgtgatgg tggtcagcgg caggaagaac atggcaacgc ctgccccctg ccaaaactgc
     5341 ggccagatga cgttgccgat atccatgtcg gcataaaaat cggtgcgcca atagaaagtg
     5401 aaggcaaaag tcaggaagct ggcggttacg agcaggcgca tatcgatttt attgccgaac
     5461 ctgccgataa tgggggacag gaaaactggg aggatgccga cgggcgcggc ggcaagccct
     5521 gcccacgtgg aggtatagcc caggttggtc tgcaacacta acggcagcag ggtcagcgtc
     5581 cccatataca ccataaaacc caatgacgtg gcaatgacgc cgacggtaaa attccgatct
     5641 ttaaacagcg ataaatcgac aatcggatat ttttctccca attcccaaac aataaaatac
     5701 gacaagcaca ccaatgcggt tacgcccaag gtaatgattt ctcccgaagc gaaccagtcg
     5761 agttccttac ccctgtccag catcatctgt aacgcgccga taccgactac catcaatgta
     5821 agcccgacat agtcggtcgg cattttaacg gtttccgttt cccgatattt caaatgtttc
     5881 catgtaatcc atgccgatat gatgccgatg gggatgttga tgaagaaaat ccaaccccaa
     5941 tgccagtttc cggaaatcca gccgccgagt atcggcccga gaacaggggc gacaacgacg
     6001 gtcattgccc acaatgccag tgccagcgtc cgttttgcgg gcggatagga tgccattaac
     6061 aggctttgcg acaggggaat cagcggcccg gcgataaagc cctgcaaaat gcggaaaaac
     6121 accaaagcct gaaggttggg cgcgatgccg cacagccacg atgtgatgac gaaaccgacg
     6181 gcggcggcgg taaacaattt gacctcgccg atacgttttg ccaaaaagcc cgttaacggc
     6241 acggaaacgg cgtttgccac agcaaaagaa gtaatgaccc acgtcccctg cgtggttgcc
     6301 gcaccgaggt tgccggcgat aacgggaacg gcgacattgg cgatagtcgt atctaaaact
     6361 tccataaata cggcaagccc caaagacagc gtaatccatg ccaatgccgc acccttaagc
     6421 ggtggataat ccattgtgtc ctccgtttca gacggcatca gggcgcggat tgcccgagga
     6481 tttcatcgac cgtccgatcg acttccgacc agtcggtact ttccatttcc ggcaatgccg
     6541 cacccggcgt ttttgaaaca ggcgcgcctg cggcagaagt atccacttta accgtcatcg
     6601 acaaaccgat gcgcaaggga tgcctgtcca catcttcgcg gttcagcacg atacggacgg
     6661 ggacgcgctg caccacttta atccagttgc ccgttgcgtt ttgcgccgga atcagcgaaa
     6721 acgcgctgcc cgtacctgcc gaaaaccctg ccaccctgcc gcgataaaca atttgtttgc
     6781 cgtacaaatc ggacaccagc tcggcaggct gtccgatttt catatgccgc aactgcgtct
     6841 ctttaaaatt ggcatccacc cacacatccg acagcggcac caccgccatc agcggcgcgc
     6901 ctgccgccac ctgctgcccg acctgcaccg aacgcttcgc cacctgaccg tccgccggcg
     6961 cgcggatttg cgtccgctga aggttcaacc acgcatcttt caacctgccg attgccgtct
     7021 gaaccgccgg ctgttcgcgc aaagaaacct gaccgcccaa agccgcgcgt gccgaagatt
     7081 cttccgccaa agccgctttg accgccgcct gcgcctgaga cactgccgca cgggcgtgtg
     7141 ccagctcttc ggcggacacc gcgcccgatt ccgccaaagc agaccggcgg cgcaaatcat
     7201 cctgcgcgcg tgccaaatcc gcacggcgca aggcaacctg cgcccccgcc tgcgaagtgg
     7261 cggcattttg ccggcggttt tgccgcaccg cctgaatcag ctcgtttttt gcccgctcgt
     7321 aagccagcac atcattatcg tcgtccaata ccgccagcac gtcgcctttt ttcacggcat
     7381 ccgtatcgtc gtgcaaaacc ttccgcaccg taccgccctt ttgcggcgta acctgaacca
     7441 cgcgcccagc aacataagcg tcttccgttt cttcttcgtg ctgccaccat aaaaaaaacg
     7501 ccgacccggc ggctgcggcg gcaagcgcga acagcagcgt caatgccgtc aggcggcgtt
     7561 tgcgtttggc ttttgcgcta tcggcttctt gtctgttttg aattttcact tcgtctccgt
     7621 gtgttgccat tattccaatt ccgaatattt ttaatacgat ataaataaag ccgatcaaag
     7681 gcagctcgga ttctataccc tggcaataac ccaatcaagt taatgattta gaatgaatat
     7741 tcaaataaac cgccacaccc gcgttccgca aaattaattt atgttcaaaa ataatcggat
     7801 aagcgcgccg taccgggcgg ggacgctaca cagataccgc cgcttatatt acaatcgtcg
     7861 ccccgtggtt cgaaaacctc ccacactaaa aaactaagga aaccctatgt cccgcaatac
     7921 cgaagagctg caaggcatct cgcttttggg taatcaaaaa acccgatatc cgaccggcta
     7981 cgcgcctgaa attctcgaag cattcgacaa caaacatccc gacaacgact atttcgtcaa
     8041 attcgtctgc ccagagttca ccagcctctg ccccatgacc gggcagcccg acttcgccac
     8101 catctacatc cgctacattc cgcacatcaa aatggtggaa agcaaatccc tgaaactcta
     8161 cctcttcagc ttccgcaacc acggcgattt tcatgaagac tgcgtcaaca tcatcatgaa
     8221 agacctcatt gccctgatgg atccgaaata catcgaagta ttcggcgagt tcacaccgcg
     8281 cggcggcatc gccattcatc ctttcgccaa ttacggcaaa gcaggcaccg agttcgaaac
     8341 attggcgcgt aaacgcttgt tcgaacacga cagccaataa accatccttc ccatatttca
     8401 gacggcattc cttcgatacg atatgccgtc tgaacgcctt tccgaaagat ttttatgtac
     8461 gcatttactg ccgcacagca acagaaggca ctcttctggc tggtgctttt ccatatcctc
     8521 atcatcgccg ccagcaacta tctggtgcag ttccccttcc aaattttcgg catccacacc
     8581 acttggggtg cgttttcctt tcccttcatc ttcctcgcca ccgacctgac cgtccgcatt
     8641 ttcggttcgc acttggcacg gcggattatc ttttgggtga tgttccccgc ccttttgctt
     8701 tcctacgtct tttccgtttt gttccacaac ggcagttgga caggcttggg cgcactatcc
     8761 gaattcaaca cctttgtcgg acgcatcgcc ttggccagct ttgccgccta cgcgctcgga
     8821 caaatccttg atatttttgt gttcaacaaa ttacgtcgtc tgaaagcgtg gtggattgca
     8881 ccgaccgcat caaccgtcat cggcaatgca ctggacacgt tagtgttttt tgccgttgcc
     8941 ttttacgcca gcagcgatga atttatggcg gcaaactggc agggcatcgc ttttgtcgat
     9001 tacctgttca aacttaccgt ctgcggtctg tttttcctgc ccgcctacgg cgtgattctg
     9061 aatctgctga cgaaaaaact gacaaccctg caaaccaaac aggcgcaaga ccgccccgcg
     9121 ccctcgctgc aaaatccgta aaacagccct gccgggaatc gggacggttg ggttttcttt
     9181 tatttgcaaa cactgtcgga acggataaat ccggattccc gcccacttcg tcattcccgc
     9241 gaaagcggga atccggtacg ttgggtttca gtcatttccg ataaactcct accgcattgt
     9301 atttctagat tcccgctttc gcgggaatga cggcggatgg gtttccgtgc cgtggtgtcc
     9361 ggattcccga cggtacggga atgacggcag tgcttcctta acttagtttt attttaatcg
     9421 actgtatggt ggattaacaa aaatcaggac aaagcgacga agccgcagac agtacaaata
     9481 gtacgaaacc gattcacttg gtgcttcagc atcttagaga atcgttctct ttgagctaag
     9541 gcgaggcaac gacgtactgg tttttgttaa tccactataa atgccgtctg aaaacctgtt
     9601 tgcaggtttt cagacggcat tgttttcgtc gccggttcaa acttcaatcc gattgcgaca
     9661 cggtaaccgg tatgccgcgc aagcgcgcaa gcagcgcgtc cgccttctgt ttctgcgcgg
     9721 caaacgccgc cttgtccgcc tgcgtcaatt cgggtgtcgg caatgcgacc gaaacaggat
     9781 tgacgggctg cccattgatg cgcgcctcgt aatgcaggtg cggccccgtc gaacgcccgg
     9841 tagaaccgac aaaaccgatg acctcgccgc cgcgcacatt gccttgtgcc tgcgaaaacg
     9901 cgctcaagtg cgcatacagc gtttccacac cgttggcgtg gcgtatcatc accgcgttgc
     9961 cgtagccacc cttccggcct ttaaaggtaa tcacgccgtc ggcggaagcc ctgaccggcg
    10021 ttccctgcgg tgcggcataa tcgatgcccg tgtgcagccg ccaagtgtgc aggatggggt
    10081 gcatacggta gccgaacggc gaagaaatgc gcgtatagac cagtggctcg atgttgaagc
    10141 cgcctttttc ctgcaacacc ctgccgtctt catcgtaata attgccgccc cctcctcctt
    10201 ccttgtccga acggtaatag aacgcctgat gccttgtgcc gcccttaacg acttccgccg
    10261 ccagaatatc gcccgccgcc acctgctgcc cgtggaaata caggctgtcg taaatcaggc
    10321 gcacggcatc gccttccttc aaaccatcaa ggctgaagcg gccggcgaaa atcccgctta
    10381 aggattcgcg aatttcgacg ggcacttccg cccgcgccag cgaaccgcgc gccgacgttt
    10441 tgaccacgac cgaacgcagc gtcggcaaaa ccttcatatc cgcctcagaa gccgaccgcc
    10501 gccatatgcc gccttttttt tccaaagcga ccagattgcg ctcgccgtct tcgtcggtaa
    10561 aaaactgcac ttcgcgcgcg ccgccgtcgc cgccgaccaa aacatgaacc gactggtcgg
    10621 cacgcaaatg ccgcaaatcg gcttcgccgc catatttttc cgttattcgg gcaatttcgt
    10681 cccgcgccat acccgaacgc gccagcacgt ccgccagcga gtcgcctggc tgcaccgcct
    10741 cctgcaccca atatgccgtc tgaacaccgc tgccgcccca agacagcggc ggcagttttt
    10801 gttccacgcg ctgcggtctg acgcgctccg tcctctctgt cgaagcaatg tatgccgccg
    10861 acaccaaaat aatcgaaacg gcaagcgcgc gcagcgcgta tttccgatgt tttgccgaaa
    10921 gtgggaagac agccatattg ttcctttttg acggcgtttg cgccgaatcc tctataatgt
    10981 tgcgcctgac ctgcatttct gaaacgtcaa acgatgaaac tcaccctcgc cattccctcg
    11041 ctcaatttgg ataaagacga aacccgaata cctctatgcc tgcccgcctt caacaaaatc
    11101 ctgcaatacg gctcgctgca ccgccaaagc tgcacggctt cggcatttta cgcgcgttat
    11161 ttgtggtgcg gcagactggc ggaacgcgcg gcacaaagcc tgaatatgcc gtctgaaacc
    11221 gtcgcgctcg ccacgcccgt ctggcaaaag atggggatgc atcaggcaaa tgtgctgacg
    11281 gcggaatatt tggatgtcgg tacggatgaa gcggaacggc tctgccgcga tctgtcggcg
    11341 ttttacggcg atattccgtg gcgtttcgtt cccgtcctgc ccgaattatg gctggtttcc
    11401 cttccccgcg cgtaccgctg gggcgcgaag cccgttttgg acttgggcgg actgttgggt
    11461 gcggacgatc aaccggacgg ggaagacgcg ttggaatggc tgcgcgtcca aaccgaaatc
    11521 caaatgtggc tggcggcgca ccccgtcaac cataaccgca agaaacgcgg gctgcccgag
    11581 ttgaacggct tatggctttg ggacagtctg cacggaagca gtcaaggcgg cacgcttttc
    11641 gccgatacgg tttggagccg tttctacccc gaccgccgcg cattgcccga caacttccgc
    11701 gcctatgaag aaacggcggc acacctgccc gacacgcacc atatcctgtt tatggacgat
    11761 ttgcgcctga ccgccctgac aggggacagg gagcggtatg catcggtttt acagcagtgg
    11821 gaagaacact ggttcgcacc gatctacgaa gccgtccgta ccggcaaaat caaacggctg
    11881 gacatcacca cggacggaca acacggcggt acgctgacct tcaaacccgc cgacaggtgg
    11941 aaattttggc gcggcgcgaa aacctttgac ggaatctggt gaacccgccc gccgcaaccg
    12001 cgacaatgcc gtctgaagcc cgaatcgggt ttcagacggc attttacctc gcatcgccgt
    12061 tatgccggaa aaaccggaat gcgtttccgc acacgcggcg gcacgcaaag caccgcccga
    12121 aagccgccga tgcccgccgt gataccgccg ccggcagggt ttcctccgaa aaggaacagc
    12181 aatcagaacg ttttattaaa ctcgacaaac gcccgatttt tctcgtattc gttgaacaca
    12241 tcgttactcc gcgtttcgcg gtgcgacaac gtcaggcgcg gcgtgatgcc tttgaaatgc
    12301 aatgcccggt gccaaaggct caaggatgtg ttcaattctt tatccctgcg cctttcccct
    12361 ttaaaaccgc tgaaaaagcc gggtttttca taatgccgtt tcgccgcgcc gaggcgcaac
    12421 agcgaagaca ggccgctgcc gccccattcc tgcccccagg caaagcgcag gccgtaacgg
    12481 ttgaaattgt cgccccggtc ggcggggttg cgctcgcggt aaaaatccaa accgcccatc
    12541 caatattggc gcgcattccg gtaaaacacc agcgaattgg aaatttgcaa atgggtattg
    12601 tcggaacgcg cccggcgcgt attcttcaaa cgcccccact ccgccgaaga caacgtttgc
    12661 catttcgggg tttgccaacg gttgaaataa aggcgtgcgc cgttggtgta agaataagcg
    12721 tcgttgccgt aggtgcggcg ttcgtggaac actgccagcc cggcatcttt gcgccggtcg
    12781 gcaaaaccga tgccgccgga aacgcctgcc gtcatatcgt tgaatttctt attccccgga
    12841 taaaccctgc cggacacgtc gccgcccgcc gtcgtgtacc agccgttttt cagcgaccat
    12901 tttttctccg cgccgagccg gtaattgacc gccgtgccgt ccacctgttt cgggaaagtc
    12961 catttgccgt actgctgccg tttcggggct tggttgatat tgtgttcgcg ggtaacgctg
    13021 aagccgccat ttaccttcca cgcatcgcgt tcgcgcaatg ccttgcggta cagctcgacc
    13081 tgctccatca gctgcggcgg caggttttcc gccttcaggc ggtcgaactg gtctgccgcc
    13141 gcctcgttct gcctgttgtc aaacaatgcc gccgccaaac gcatacggac ggcgggcgcg
    13201 tcgggttggg cggcaatcaa ttcccggtaa tgggaaatcg cctccttcac cctaccgtct
    13261 gcctgcgcca aaatcccttg tgcataaagt gccaacatct tatcctgctg cgcctgttgt
    13321 aggtaaatcg gcaaaataac gcggataccg gcaatattgt ttgagaccac tgcggaatac
    13381 atcgcgcggg acaacaattc gggacttttc agcagggttt ctccgtcaat ctgcaaaact
    13441 tttccttttt cccgcacctg ccccggtacc ttctccctgt cgattggttt gacctccgct
    13501 tcatgaagcc tgaactcggg acggcttctc aaatccggtt cgcgcggtgt ttcttcggca
    13561 tatgccgcac ttgccaataa aggcagcagc agcatccatc tgtttcgtgc aggcataaat
    13621 gtcttcccac aaaaataaaa aataaccatt atcaatcggc ggattgtatc agttttttat
    13681 gccgctgttc caaaccctgc cgcccgatgc cgcgacaatg ccgtctgaag ccagaatcgg
    13741 gtttcaaacg gcggcaaaaa accggcggtt ttccgccggt ttcatcatta tgccccgccc
    13801 ttcggggcag cagggttcgg attggtttgc ggggcggcgt gcggtgccgt gccggtgtgc
    13861 cgcctgtacg tcaaaacatt tcccgacgca tcaaaacagc catttgagcg acaatgcggc
    13921 ttctttgtcg ccgtccgtcc ttttgccgta tccgatgcgt gcggacatat gacctttcca
    13981 accggcttca atgccgaacc gcccttcgag cgccgtcctg cctgccagcg tctgtttttc
    14041 gccgtccatt tccacgccga aagattttga cctgtgcaaa acattaaaag cggcaaaagg
    14101 ctgaagattg acaccattac gcaaagcaaa acggtttttt gcccgaatgc cggcgcggct
    14161 ttgccactga ccgctgccga gcagtccgac ccccgtcccc tcgctgtcgg taaagccgcc
    14221 gtttacgccc aagtaggtaa actgcgcctg cggttgcagg taaaaccgca cattattgcc
    14281 ttttccgaca actccttccg ccacaagcgt gttgtagccg ccttcgacag aagccgtcca
    14341 acctttggtt ttgtagcgtt ccgcacggtt ttcatcattg atgcggtgtt tgaaacgttg
    14401 gtattgcaac cagccgtcca aatacgcacc cgtttgttta tcgcgcaact gatgccacgc
    14461 agcataaaca cccccgccat aaccatgcaa atcactgcct gccgcaccgc ctttgctgtt
    14521 aactgatgcg tgctggccga ccctgccgcc catcacgccg attgccagcc ggctgccttc
    14581 attttgccgt acaaacacct cgccgccgat ttgcacgcct ttgcgccgcc cgtccgcagc
    14641 cgcgccaccc cgtatatttt gacgggacgg atcggcggca tacacgtcgt cgcccctgtc
    14701 ggcggcacgc agcccaaaca aagtatttgc cgcataagcc tgttgggcat ataaaaccgc
    14761 ttccgcctcg gggatagcca aacgctgcac gcccaagcaa aatccgcctt cgcattgctt
    14821 gagcaataat tcgtacagtc cctgccggta attttgttta aacgcaaacg aaccggaatc
    14881 cgccgttttt gccgcaatca gtttgcgccc gtcgagcgtt ttcagatttt ccggccggcg
    14941 ttccaaaacc acgccggatt gtccgctgac tttgtcttca aaggtcaggg cgggttcgtc
    15001 cgactcccgc acgtcgtagc cgaacaggaa atccgcgccg cccgcataat ccttatggac
    15061 ggtcagtttt tcatctttcg tgttcaggcg gatgcgggca ttatcggaag tttccagcgt
    15121 atggatgtcg gaatggcggc gcggttccca aagggaatct ttcagcgtca tcttggtcag
    15181 ttccaacacc tgtccggcga cgtgggattt gttttcgatt ttgacgtgta attttttatt
    15241 gaagatgttg ttgagactag tctgtgatgt attactatga ggagtgagta cggaatcttc
    15301 taccaattta ttaattaaat cggggttttt ctgtttgagt atactaaatt ttggtccaag
    15361 ctgttctata tacgcctcct cagtccgttt tttattttct tcccaagctt cgggtcttgt
    15421 tttgtataaa tcctgtaatt gcttcttaac cgcatccttt aaattatccc cgtaagtatc
    15481 tgtaatatat ttcttaacaa cgggcatatt atcgacggcg agtatgccgc tattgtagtt
    15541 tttaccttgg atattgattt cgtcataccc atattcggat gaattttgat cggttttacc
    15601 gctggaaaaa taatgggaag tgccgggttt cggcttgtct gaaaatgaaa aatcggcatc
    15661 gtcgccgatt ttggtagaac gagtatttga accaagctga gctctgaaca taccgaagca
    15721 atcaaaagat atgctgcacg ggccgcgcca aacgtgggaa tttctgtctt ccgccgcttc
    15781 taaaataaat ttgtttttaa tctcggcttc tttgacggga tcgtacgcat ccgaacctca
    15841 aacatatggc gtgcaaaaca gcgataaaac agataatgtt aatagattaa cccgac
//
LOCUS       DAWKST010000034        15618 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.034, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000034 DAWKST010000000
VERSION     DAWKST010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15618
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.034"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            582..1457
                     /gene="prpB"
                     /locus_tag="WJL87_000743"
     CDS             582..1457
                     /gene="prpB"
                     /locus_tag="WJL87_000743"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ7799203.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            1543..2697
                     /gene="prpC"
                     /locus_tag="WJL87_000744"
     CDS             1543..2697
                     /gene="prpC"
                     /locus_tag="WJL87_000744"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ7799204.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            2790..3512
                     /locus_tag="WJL87_000745"
     CDS             2790..3512
                     /locus_tag="WJL87_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEZ7799205.1"
                     /translation="MDKDKGSYAGIPVQNTKELVWQKIQDIPISECGEPLVSVGNTGR
                     IRVNPVYFSQGIEGAINSVLLRRSVADRLTQVVSLLPEVYGLEIFDGWRPVIVQRALR
                     ENMKQEIEKRHPEYDEVQVREALDCFAADPDRAGMPLPHLTGGSVDVGLFDIKSGKSL
                     DMGSEFDATGHLSYSDALEKEGEYFSSARNLRRILIHAMKEAGFSNLPTEWWHFDYGN
                     QNWAYFTQQSNAVFGAVEEQDT"
     gene            3538..4743
                     /locus_tag="WJL87_000746"
     CDS             3538..4743
                     /locus_tag="WJL87_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HEZ7799206.1"
                     /translation="MQTLISADNHLLLWAFVMLAAAAAIFIEQNSRLAGKVPGAVLAL
                     LIALIASNLGVIPTDAPVFDAVWSYIVPLAIPLLLFQLDLHALFKESGRMLFIFLISS
                     LGTVIGTLVSFALLKNYIPELDKVAGMISASYTGGGVNFAAMSAKLNPAKDVIAATIV
                     ADNLMMACYFFILIGISSSYWVRKVWGSPYQDKLEQEYHDPSVNKAAEYWKPKSISLQ
                     SIALSLAASILLVAVSFSLSSFLQNHFQSVDGVALKMLTGLISDKYLLLTTFTLAVVL
                     FFRKGIQKLDGSQELGTYCIYLFFVVIGVPASIPLIIKTAPLLFVFTLIIAVLNLLLT
                     LVLGKLFKFSIEEIVLACNANIGGPTTAAALAISKGWRDLVGAILLIGTVGYIIGNYI
                     GSFVYALLS"
     gene            4846..7452
                     /gene="acnD"
                     /locus_tag="WJL87_000747"
     CDS             4846..7452
                     /gene="acnD"
                     /locus_tag="WJL87_000747"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ7799207.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNREIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDALGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELNGKRQAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLASKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRFGLKRKPWVKTSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRVHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLSGMRPLAILPDNITTDHLSPSNAILASSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVRNEDGSVRQGSLARVEPEGQTMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPGTNRH
                     TLQLDGTETYDVVGERKPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            7805..8974
                     /gene="prpF"
                     /locus_tag="WJL87_000748"
     CDS             7805..8974
                     /gene="prpF"
                     /locus_tag="WJL87_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ7799208.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFAIEQGLVDKSKIPSDGICTVKIWQKNIGKTIIAHVPMQHGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTIFLN
                     AVDLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            9388..10587
                     /locus_tag="WJL87_000749"
     CDS             9388..10587
                     /locus_tag="WJL87_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ7799209.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAVGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMDKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            10694..11314
                     /locus_tag="WJL87_000750"
     CDS             10694..11314
                     /locus_tag="WJL87_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ7799210.1"
                     /translation="MAQFFAIHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKEVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVIDMTDGTELVRQGCGDTAVFGL"
     gene            complement(11476..11919)
                     /locus_tag="WJL87_000751"
     CDS             complement(11476..11919)
                     /locus_tag="WJL87_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ7799211.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPDRINIGSVVRHLEPMQLVECMGENNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            12093..12461
                     /locus_tag="WJL87_000752"
     CDS             12093..12461
                     /locus_tag="WJL87_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ7799212.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFA
                     ELETHFREEEAKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            12506..13660
                     /locus_tag="WJL87_000753"
     CDS             12506..13660
                     /locus_tag="WJL87_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ7799213.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVIAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(13717..14589)
                     /locus_tag="WJL87_000754"
     CDS             complement(13717..14589)
                     /locus_tag="WJL87_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ7799214.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHHCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALVEELWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            14674..15486
                     /locus_tag="WJL87_000755"
     CDS             14674..15486
                     /locus_tag="WJL87_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ7799215.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGVDWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTISAGREVPHLSAEGVSVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKYRVLDAV"
BASE COUNT         3707 a         3980 c         4216 g         3715 t
ORIGIN      
        1 aattcaatcc actatatatt taaatttaat taaataatat aaaaatcaaa tagataacaa
       61 actacataag attacttaaa aataatacat tgtttttatg tataaaatat tttataagtt
      121 ttcaggattt tgattattga aaatttttct tgatttcctg acaattttat tgaaacaaat
      181 aattcaaaat taatctagtt taatcataga attaaaataa aatattaaaa ttatgtaatg
      241 agtctcctta aaaatgtttg acattttcag tcttgtgttt tagattatcg aaaaataaaa
      301 ctacataaca ctacaaagga acattactat gaaaccaatt cagatgtttt ccccttttct
      361 gaataatccc cttgttttct tcttgtctgc ggttttgccg cataattccg aacggtctgc
      421 tgtttttctt tgattcgttt taaatatcaa taagataatt gttcccatat atttttaatg
      481 attggattgg gatgcccgac gcgtcggatg gctgtgtttt gccgtccgaa tgtgatggaa
      541 gcctgtccat actgaaaaaa agtctataaa ggagaaatat gatgagtcaa cactctgccg
      601 gagcacgttt ccgccaagcc gtgaaagaat cgaatccgct tgccgtcgcc ggttgcgtca
      661 atgcttattt tgcacgattg gccacccaaa gcggtttcaa agccatctat ctgtccggcg
      721 gcggcgtggc agcctgttct tgcggtatcc ctgatttggg cattaccaca atggaagatg
      781 tgctgattga cgcgcgacgc attacggaca acgtggatac gcctctgctg gtggacatcg
      841 atgtgggttg gggcggtgca ttcaatattg cccgtaccat ccgcaacttt gaacgtgccg
      901 gtgttgcagc ggttcacatc gaagatcagg tagcgcaaaa acgctgcggc caccgtccga
      961 acaaagccat tgtatctaaa gatgaaatgg tcgaccgtat caaagctgcc gtagatgcgc
     1021 gcgttgatga gaacttcgtg attatggcgc gtaccgatgc gctggcggta gaaggtttgg
     1081 atgccgctat cgaacgcgcc caagcttgtg tcgaagccgg tgcggacatg attttccctg
     1141 aagccatgac cgatttgaac atgtaccgcc aatttgcaga tgcggtgaaa gtgcccgtgt
     1201 tggcgaacat taccgagttt ggttccactc cgctttatac ccaaagcgag ctggctgaaa
     1261 acggcgtgtc gctggtgctg tatccgctgt catcgttccg tgcagcaagc aaagccgctc
     1321 tgaatgttta cgaagcgatt atgcgcgatg gcactcaggc ggcggtggtg gacagtatgc
     1381 aaacccgtgc cgagctgtac gagcatctga actatcatgc cttcgagcaa aaactggata
     1441 aattgtttca aaaatgattt accgctttca gacggtcttt caacaaatcc gcatcggtcg
     1501 tctgaaaacc cgaaacccat aaaaacacga aggagaaata ccatgactga aactactcaa
     1561 accccgacct tcaaacctaa aaaatccgtt gcgctttcag gcgttgcggc cggtaatacc
     1621 gctttgtgta ccgttggccg taccggcaac gatttgagct atcgcggtta cgacatcttg
     1681 gatttggcac aaaaatgcga gtttgaagaa gtcgcccacc tgctgattca cggtcatctg
     1741 cccaacaaat tcgagctggc cgcttataaa accaagctca aatccatgcg cggcctgcct
     1801 atccgtgtga ttaaagtttt ggaaagcctg cctgcacata cccatccgat ggacgtaatg
     1861 cgtaccggcg tatccatgct gggctgcgtt catcctgaac gtgaaagcca tccggaaagt
     1921 gaagcgcgcg acatcgccga caaactgatc gccagcctcg gcagcatcct cttgtactgg
     1981 tatcaatatt cgcacaacgg caaacgcatt gaggttgaaa gcgacgaaga gaccatcggc
     2041 ggtcatttcc tgcaactgtt gcacggcaaa cgcccaagcg aatcccacat caaagccatg
     2101 cacgtttcac tgattctgta tgccgaacac gagttcaacg cttctacctt caccgcccgc
     2161 gtgattgccg gtacaggctc tgatatgtac tccagcatta ccggagcaat cggcgcgttg
     2221 aaaggtccga aacacggcgg cgcgaacgaa gtggcttacg atattcaaaa acgctaccgc
     2281 aatgccgacg aagctgaagc cgacatccgc gaacgcatcg gccgcaaaga aatcgtgatc
     2341 ggtttcggtc atccggtgta caccatctcc gaccctcgca acgttgtcat taaagaagtg
     2401 gcacgcggtt tgagcaaaga aaccggcgat atgcgcctct ttgacattgc cgaacgtttg
     2461 gaaagcgtga tgtgggaaga gaaaaaaatg ttcccgaatc tggactggtt ctctgccgtt
     2521 tcctaccaaa aattgggcgt accgaccgct atgttcacac cgctgttcgt aatttcccgt
     2581 acaaccggtt ggagcgcaca cgttcttgag caacgcaaag acggcaaaat catccgtccg
     2641 agcgcaaact acacgggccc tgaagatttg gcgtttgtgg agattgaaga acgctgatga
     2701 ttttcagacg accttgtttg tacaaggtcg tctgaaaaaa gaaatttaat gaatttattg
     2761 gcacactaat tattaatgcg gaacatttga tggataagga caaggggtct tatgcgggta
     2821 ttcctgtaca aaataccaaa gagttagttt ggcagaaaat acaagacata cctatttctg
     2881 aatgtgggga accgttggtc agtgttggta ataccggacg cattcgtgtc aatcctgttt
     2941 atttttctca gggtattgaa ggggcgataa atagtgtttt attgcgtaga agtgttgctg
     3001 ataggctgac tcaggttgtc tctttgctgc cggaagtgta cgggttggaa atttttgatg
     3061 gttggcgtcc tgtcatagtg caaagagcat tacgagaaaa tatgaagcaa gaaattgaaa
     3121 aacgccatcc ggaatatgat gaagttcaag ttagagaagc actggactgt tttgctgctg
     3181 atccagatcg ggcaggaatg cctctgcccc atttgacagg cggatctgtt gatgtcggat
     3241 tgtttgatat aaaaagtggc aaaagtttgg atatgggaag tgagtttgat gcaactggac
     3301 atttatctta tagcgatgct ttagagaaag aaggtgagta tttctcctct gctcgtaatt
     3361 tacgccggat tttgattcat gcgatgaaag aggcaggatt tagtaatttg ccaaccgaat
     3421 ggtggcattt cgattatggc aaccaaaatt gggcttattt tacccaacaa agcaacgcag
     3481 tttttggggc ggtagaagag caggatactt aaattaaaat aaaaattggg aggaaaaatg
     3541 caaactttaa tttcagcaga taatcatttg ctgctgtggg cttttgttat gttagctgcc
     3601 gctgctgcga tttttattga gcaaaacagt cgcttggctg ggaaagtccc tggtgcagta
     3661 ttagctttat tgattgcgtt gattgcttct aatttgggtg ttattccgac ggatgctcca
     3721 gtatttgatg ctgtatggtc ttatattgta cctcttgcta ttccgttgtt attgtttcaa
     3781 ttagatcttc atgctctatt taaggaaagt ggacgtatgc tgtttatttt cctgattagt
     3841 tcactaggga cggtcattgg aacgcttgtc tcatttgcac tattgaaaaa ctatattcct
     3901 gaactagata aggtagctgg catgatttca gcatcttata cgggaggtgg tgttaatttt
     3961 gcagctatgt cagctaagct taaccctgct aaagatgtta ttgcagcaac aattgttgct
     4021 gataatttga tgatggcttg ttactttttt attttgattg gtatctcttc ctcatattgg
     4081 gtcagaaaag tttggggaag tccatatcag gataaattgg agcaggaata tcatgatccg
     4141 agtgtgaata aggctgccga gtattggaaa cctaaatcca tttcgctaca gagtatagcc
     4201 ttatctttgg ctgcctcgat tttgttggtc gcagtttcat tctcattatc cagttttttg
     4261 caaaatcatt ttcaaagtgt tgatggtgtt gcattaaaaa tgctgacagg tttaattagt
     4321 gacaaatatt tgctgttaac gacatttaca ttggcagttg ttttattttt ccgtaaaggc
     4381 attcaaaaac tagatggcag tcaagaatta gggacatatt gtatttatct gttttttgtt
     4441 gtgattggtg ttcctgcctc tataccgtta attattaaaa cagcaccatt actctttgtt
     4501 tttactttga ttattgcggt attgaatttg ttactgacgt tagtgttagg caagctgttc
     4561 aaattcagta ttgaagaaat tgtattggct tgtaatgcca atattggagg gccgactact
     4621 gcagcagcct tagccatcag taaaggttgg cgtgatttag tcggtgcaat tttgttaatt
     4681 ggtactgtag gctacattat cggtaactat ataggttcgt ttgtatatgc cctattgagc
     4741 tgaagactaa tctaacaatc catgaacatt ttcagacgac ccccttccaa aaacgtcggc
     4801 tgaaacctca aaccacaaga aaaacagatc cccaggagaa ccgacatggc tgccaaccaa
     4861 cgttaccgca aaccgctgcc cggtacggat ttggaatact acgacgcgcg tgcggcgtgt
     4921 gaggacatca agcccggctc ttacgacaag ctgccttata caagccgtat cttggctgag
     4981 aacttggtca accgcgcgga caaagtcgat ttgccgacgc tgcaaagctg gctgggccag
     5041 ttgatagaag ggaagcagga aatcgacttt ccgtggtatc ccgcgcgggt ggtgtgccac
     5101 gatattctgg ggcagaccgc gttggtggat ttggcaggcc tgcgcgatgc gattgccgaa
     5161 aaaggcggcg atcctgccaa agtgaatccg gtggtgcaaa cccagctcat cgtcgaccac
     5221 tcgctggcgg tggagtgcgg cggttacgat cctgatgctt tccgcaaaaa ccgcgaaatc
     5281 gaagacagac gtaacgaaga ccgtttccac ttcatcaact ggacaaaaac cgcatttgaa
     5341 aatgtggacg tgattccggc gggcaacggc atcatgcacc aaatcaatct agaaaaaatg
     5401 tcgcccgtcg tccaagtcaa aaacggcgtg gcgttccccg atacctgcgt cggcacggat
     5461 tcgcacacgc cgcacgtcga tgcgctgggc gtgatttccg tgggcgtggg cggattggaa
     5521 gcggaaaccg taatgctggg acgcgcgtcc atgatgcgcc tgcccgatat tgtcggcgtt
     5581 gagctgaacg gcaaacggca ggcgggcatt acggcgacgg atattgtgtt ggcgctgacc
     5641 gagtttctgc gcaaagagcg cgtggtcggg gcgtttgtcg aatttttcgg cgagggcgcg
     5701 agaagcctgt ctatcggcga ccgcgcgacc atttccaaca tgacgccgga gttcggcgcg
     5761 actgccgcga tgttcgctat tgatgagcaa accattgatt atttgaaact gaccggacgc
     5821 gacgacgcgc aggtgaaatt ggtggaaacc tacgccaaaa ccgcaggctt atgggcagat
     5881 gccttgaaaa ccgccgttta tcctcgcgtt ttgaaatttg atttgagcag cgtaacgcgc
     5941 aatatggcag gtccaagcaa cccgcacgcg cgttttgcga ccgccgattt ggccagcaaa
     6001 ggcttggcta aaccttacga agagccttca gacggacaaa tgcccgacgg cgcggtcatc
     6061 atcgccgcga ttaccagttg caccaacact tccaacccgc gcaacgtcgt tgccgccgcg
     6121 ctcttggcgc gcaacgccaa ccgcttcggg ctgaaacgca aaccgtgggt caaaacctcg
     6181 tttgcccccg gttcgaaagt ggcggaaatt tatttgaaag aagcaggcct gctgcccgaa
     6241 atggaaaaac tcggcttcgg tatcgtcgcc ttcgcctgca ccacctgcaa cggcatgagt
     6301 ggcgcgctgg atccgaaaat ccagaaagaa atcatcgacc gcgatttgta cgccaccgcc
     6361 gtattatcag gcaaccgcaa cttcgacggc cgtgtccatc cgtatgcgaa acaggctttc
     6421 ctcgcttcgc ctccgttggt cgttgcctac gcgctggcag gcagtatccg tttcgatatt
     6481 gaaaacgacg tactcggcgt tgcagacggc aaggaaatcc gcctgaaaga catttggcct
     6541 gccgatgaag aaatcgatgc cgtcgttgcc gaatatgtga aaccgcagca gttccgcgat
     6601 gtgtatgtac cgatgttcga caccggcaca gcgcaaaaag cacctagtcc gctgtacgat
     6661 tggcgtccaa tgtccaccta tatccgccgc ccaccttact gggaaggcgc attggcaggg
     6721 gagcgcacat tgagcggtat gcgtccgctg gcgattttgc ccgacaacat caccaccgac
     6781 cacctctcgc catccaatgc gattttggca agcagtgccg caggcgaata tttggcgaaa
     6841 atgggtttgc ctgaagaaga cttcaactct tacgcaaccc accgcggcga ccacttgacc
     6901 gcccaacgcg caacctttgc caatccgaaa ctgtttaacg aaatggtgag aaacgaagat
     6961 ggcagtgtac gccaaggttc gctggcacgc gttgaacccg aaggccaaac catgcgcatg
     7021 tgggaagcca tcgaaaccta tatgaaccgc aaacagccgc tcatcatcat tgccggtgcg
     7081 gactacggtc aaggctcaag ccgcgactgg gctgcaaaag gcgtacgcct cgccggtgtg
     7141 gaagcgattg ttgccgaagg cttcgagcgt atccaccgta ccaacttgat cggtatgggc
     7201 gtattgccgc tgcagttcaa accgggtacc aaccgccaca ccttgcaact ggacggtacg
     7261 gaaacctacg acgtggtcgg tgaacgcaaa ccgcgctgcg atctgaccct cgtgattcac
     7321 cgtaaaaacg gcgaaaccgt cgaagttccc gttacctgcc gcctcgatac tgcagaagaa
     7381 gtattggtat atgaagccgg cggcgtgttg caacggtttg cacaggattt tttggaaggg
     7441 aacgcggctt agaggtcgtc tgaaaagcaa gacgtagcgt gggtcgggtt caacattttg
     7501 ctcattcacg taattctcga tatggcaggc atctactgta aatcgtcatt cccgcgcagg
     7561 cgggaatcca gaaagtggaa ttgaggaaac cttatttatc cgatgagttt ctgtgcggac
     7621 aaatttggat tcccgcctgc gcgggaatga cggggtttaa taatctgccg tatcacaaca
     7681 cagtagccgt agattgtggc gaaccccgac agtttgcgga atcaaacggc tttggtcgga
     7741 gtggcagcct aatccactat aaaaatcgtg ggcagagccc acgctacata aggagaatct
     7801 agaaatgccg caaattaaaa tccccgccgt ttactaccgc ggtggcacat caaaaggcgt
     7861 gtttttcaaa cgttccgacc tgcccgaggc ggcgcgggaa gcgggcagcg cacgcgacaa
     7921 aatcctcttg cgcgtactcg gcagcccgga cccctacggc aagcagatag acggtttggg
     7981 caacgccagt tcgtccacca gcaaagccgt gattttggac aagtccgaac gcgccgatca
     8041 cgatgtcgat taccttttcg ggcaagtttc catcgacaaa ccttttgtcg attggagtgg
     8101 caactgcggc aacctcaccg ccgccgtagg cgcatttgcc atcgaacaag gtttggtcga
     8161 taaatccaaa atcccttcag acggtatctg cacggtgaaa atctggcaga aaaacattgg
     8221 caaaaccatt attgcccatg ttccgatgca acacggcgca gttttggaaa cgggcgattt
     8281 tgagcttgac ggcgtaacgt tcccggcagc cgaagtacaa atcgaatttt tagatccggc
     8341 cgacggcgaa ggcagtatgt tcccaaccgg caatttggtc gatgaaattg atgtgccgaa
     8401 tataggccgt ctgaaagcca cgcttatcaa tgccggtatt ccaaccattt tcctgaatgc
     8461 cgtagatttg ggctacacag gcaaagagtt gcaagacgac atcaacaacg atgccgcggc
     8521 tttggaaaaa ttcgagaaaa tccgcgctta cggtgcgctg aaaatgggtc tgatcagcga
     8581 cgtatccgaa gctgccgccc gcgcgcacac gccgaaagtc gccttcgtcg cgcccgccgc
     8641 cgattacacc gcctccagtg gcaaaaccgt gaacgccgcc gacatcgatt tgctggtacg
     8701 cgccctgagc atgggcaaat tgcaccacgc gatgatgggt accgcctctg ttgccattgc
     8761 gaccgccgcc gccgtgcccg gtacgctggt caaccttgcc gcaggcggcg gaacgcgtaa
     8821 agaagtgcgc ttcgggcatc cttccggcac attgcgcgtc ggtgcagccg ccgaatgtca
     8881 ggacggacaa tggacggcca ccaaagcggt tatgagccgc agcgcacgcg tgatgatgga
     8941 aggttgggtc agggtgccgg aagattgttt ttaaattgac gtagcatggg tttgcccgcg
     9001 agccataaac aggtcgtctg aaaaacaagt aaacatcaaa tcactgacca ttcctttccc
     9061 ttgccctgtg gcggaaggcg gcaaatcaca aggaagaaca cggaaacccc gataaaagac
     9121 agcttcccgt attaccgtca ttcccgcgca ggcgggaatc tagacctgtc aatatggagg
     9181 attggtaggg gaaaacaggt ttcgtgagtt ctacattctg gattcccgcc acagcctgtc
     9241 ctcgcgtagg cggggacgga ataacgatag aaaatgcggc atatgctttg cccaaagagg
     9301 ccgtctgaaa caccttgcgc ctgatgtctg cctttttcag acgaccccac accaaaaaaa
     9361 caaccacaaa ctacaaggag aaacatcatg tccgaccaac tcatcctcgt tctgaactgc
     9421 ggcagttcat cgctcaaagg cgccgttatc gaccgaaaaa gcggcagcgt cgtcctaagc
     9481 tgcctcggcg aacgcctgac cacgcccgaa gccgtcatta cgttcaacaa agacggcaac
     9541 aaacgccaag ttcccctgag cggccgcaac tgccatgccg gcgcggtggg tatgctgctg
     9601 aacgaactgg aaaaacacgg actgcacgac cgcatcaaag ccgtcggcca ccgcatcgcc
     9661 cacggcggcg aaaaatacag cgagtctgtt ttgatcgacc aggccgtaat ggacgaactc
     9721 aatgcctgca ttccgcttgc gccgctgcac aaccccgcca acatcagcgg catccttgcc
     9781 gcacaggaac atttccccgg cctgcccaat gtcggcgtga tggatacttc gttccaccaa
     9841 accatgccgg agcgtgccta cacttatgcc gtgccgcgcg agttgcgtaa aaaatacgct
     9901 ttccgccgct acggtttcca cggcaccagt atgcgttacg ttgcccctga agccgcacgc
     9961 atcttgggca aacctctgga agacatccgc atgattattg cccacttagg caacggcgca
    10021 tccattaccg ccatcaaaaa cggcaaatcc gtcgatacca gtatgggttt cacgccgatc
    10081 gaaggtttgg taatgggtac acgttgcggc gacatcgatc cgggcgtata cagctatctg
    10141 acttcccacg ccgggatgga tgttgcccaa gtggatgaaa tgctgaacaa aaaatcaggt
    10201 ttgctcggta tttccgaact ttccaacgac tgccgcaccc tcgaaatcgc cgccgacgaa
    10261 ggccacgaag gcgcgcgcct cgccctcgaa gtcatgacct accgcctcgc caaatacatc
    10321 gcttcgatgg ctgtggcctg cggcggcgtt gacgcactcg tgttcaccgg cggtatcggc
    10381 gaaaactcgc gtaatatccg tgccaaaacc gtttcctatc ttgatttctt gggtctgcac
    10441 atcgacacta aagccaatat ggacaaacgc tacggcaatt cgggtattat cagcccgacc
    10501 gattcttctc cggctgtttt ggttgtcccg accaatgaag aactgatgat tgcctgcgac
    10561 actgccgaac ttgccggcat cttgtagcca aaaaagggac gagtccgcac aatgccgtct
    10621 gaaattttca gacggcattt tgtttggatt ttgggcgtac aatacggaaa acatgacgat
    10681 aaggacacaa accatggcac agtttttcgc tattcatccc gacaaccccc aagaacgcct
    10741 catcaagcag gcggtcgaaa tcgtcaataa aggcggcgtg gtcgtttatc cgaccgattc
    10801 ctgttatgcc ttgggttgca aactcggcga taaggcggcg atggaacgca tactctccat
    10861 ccgaaaaatc gatttgaaac accacctgac cctgatgtgc gccgatttga gcgagttggg
    10921 cacatacgcc aaaatcgaca acgtacagtt tcgtcagctt aaggccgcca cgcccgggcc
    10981 ttataccttt atcttgcagg cgacgaagga agtgccggcg cgcacgctgc acccgaaacg
    11041 caaaaccatc gggctgcgta ttcccgataa tgccattgca caagccctgc tgggggaatt
    11101 gggcgaaccg cttttaagct gcaccctgat gctgcccgaa gacggcgaac cattgaccga
    11161 tccttatgaa atccgcgagc gtttggaaca cgccgtcgat ttggtgattg acggcggctg
    11221 gtgcggaacc gagccgacca ccgtcatcga catgaccgac ggtacggaat tggtgcgcca
    11281 aggttgcggc gatacggcgg tgttcggttt gtagggaaac cgatgccgtc tgaagcatcg
    11341 gctgttcaga cggcattgcg cgccttgccg gcggcagtcc gaaatgccgg cgcgtatcgc
    11401 gctcggtcgg aatatccgtt tgaaacggca aacaaaatgc cgtccgaacg cgggttcgga
    11461 cggcattttg atgcattact gcaccgcaat cggaattctc ggttcgtaga gcaggtcgta
    11521 ggtcggcttg ttgagcaggt cttggagcgt gaaaccgtcc aaatacgtga aaaacgactt
    11581 catcgcgccg ccgagtatgc ctgtcagacg gcaggacggt gtgatcaggc attcgttgtt
    11641 ctcgcccatg cactcgacca gctgcatcgg ttcgaggtgg cggacgaccg agccgatgtt
    11701 gatgcggtcg ggcggtgcgg caagccgcag accgccgcct tttccgcgca cgctgtggag
    11761 gaagccgcct ttgaccagcg cggtaacgac cttcatcaga tggcttttgg aaatgccgta
    11821 ggttacggcg atggtactga tgttgaccag cgcatcgtcg ttgatggcag tgtagataag
    11881 gacgcgcagc ccgtagtccg tatgttgtgt caaatacatg attttctcgg tatggattgt
    11941 tattcttatc ggtacggttt aaggttcacg gacaatacct taatggttga aaccctgtcc
    12001 gtcggggcgg tagaatgcag cctgtctgcg gcggtatgcc gtctgaaaca tccgcgctac
    12061 cgtttgataa tttgttattg taactcaaaa tcatgaaacc gttgaaacga catcccgccc
    12121 ttatcgggct ttcgcgtgac caccaccatt cgctttccct gtgcgtgcgt ctgttgcgga
    12181 cgccggaaga aaggcatcgg gacgaactcg aaccgcattt tgccgaattg gaaacccatt
    12241 ttcgcgaaga agaagccaag tttgccccaa tttggcagaa tgtcgccccc gaattgaaac
    12301 aacgtttcga gaaagaccac gcccgactgc ggcagatgat ggcaagcccc gaatacggta
    12361 acgcggcgtg gaataccgct tttgccacaa ccctgcgcga ccacgcgcgc tttgaagaac
    12421 gcgagctgtt tcccgccgcc gaaccgtttt tgccggcatg attccgtttt gcggcaaata
    12481 tattaatgat aaacaaggaa cacacatgaa atttaccaag caccccgtct gggcaatggc
    12541 gttccgccca ttttattcgc tggcggctct gtacggcgca ttgtccgtat tgctgtgggg
    12601 tttcggctac acgggaacgc acgagctgtc cggtttctat tggcacgcgc atgagatgat
    12661 ttggggttat gccggactgg tcgtcatcgc cttcctgctg accgccgtcg ccacttggac
    12721 ggggcagccg cccacgcggg gcggcgttct ggtcggcttg actatctttt ggctggctgc
    12781 gcggattgcc gcctttatcc cgggttgggg tgcgtcggca agcggcatac tcggtacgct
    12841 gtttttctgg tacggcgcgg tgtgcatggc tttgcccgtt atccgttcac agaatcaacg
    12901 caactatgtt gccgtgttcg cgctgttcgt cttgggcggc acgcatgcgg cgttccacgt
    12961 ccagctgcac aacggcaacc taggcggact cttgagcgga ttgcagtcgg gcttggtgat
    13021 ggtgtcgggt tttatcggtc tgattggtac gcggattatt tcgtttttta cgtccaaacg
    13081 gttgaatgtg ccgcagattc ccagtccgaa atgggtggcg caggcttcgc tgtggctgcc
    13141 catgctgacc gccatgctga tggcgcacgg cgtgatgcct tggctgtcgg cggctttcgc
    13201 gtttgcggca ggtgtgattt ttaccgtgca ggtgtaccgc tggtggtata aacccgtgtt
    13261 gaaagagccg atgctgtgga ttctgtttgc cggctatctg tttaccggat tggggctgat
    13321 tgcggtcggc gcgtcttatt tcaaacccgc tttcctcaat ctgggtgtgc atctgatcgg
    13381 ggtcggcggt atcggcgtgc tgactttggg catgatggcg cgtaccgcgc tcggtcatac
    13441 gggcaatccg atttatccgc cgcccaaagc cgttcccgtt gcgttttggc tgatgatggc
    13501 ggcaaccgcc gtccgtatgg ttgccgtatt ttcttccggc actgcctaca cgcacagcat
    13561 acgcacctct tcggttttgt ttgcactcgc gcttttggtg tatgcgtgga agtatattcc
    13621 ttggctgatt cgtccgcgtt cggacggcag gcccggttga gacaaaccgc cgcagatttc
    13681 gggtctgggc ggtttgcttt tcagacggca gggcggtcag ttgccgtcca gccagcggtc
    13741 gcgtgtggtt ttggcttctt caaaatagcg gtacagggct tcgcggtcgt cggtggtcag
    13801 gatgtttgcc aaaacgtcca actgtttgcc caagccttga accagttgca gcaggctgtc
    13861 tttgttggca aggcagatgt ccgcccacac ggcgggatga ccggaggcga tgcgggtgaa
    13921 gtcccgaaag cccgtggcgg cgaatttcag atattcctgt ccgtcggggt ggtcgagaat
    13981 ctggtggaca taggcgaagg cggtcaggtg gggcatatgg gagacggcgg cgaaaaccgc
    14041 gtcgtggcgt tgcgcgtcca tcgtataaat ttccgcaccg accgcgtgcc acagttcttc
    14101 taccaaggca atgccgtctg aatgttcgcc gccgtgtggc gtgatgatga gttttctgtg
    14161 gcggaacagc ccgaactgcg cggcttgcgc accgcttctg tccgaaccgg caatcgggtg
    14221 ggcggcgatg cagtggtgca gacggtcggg cagacagcgg cggaaggctt cgatgaccga
    14281 agatttggtg ctgccgacat cggaaatcca agtgtgttcc ggcaaaacgg ggcgcagcgc
    14341 ggtcaaaatg gcgggaacgg tggcgacggg cgtggcaatc agtaccaagt ccgcaccgcc
    14401 gatgctgtcc gcgtcgatgg caacggacgc acggtcaatc acgccgcgtt ccaatgcacg
    14461 ttcgaggttg tcgcggtcgg tgtcgatacc ggtaacggtg cggacgagtc cctgcctttt
    14521 gaggtcgaga acgaacgaac cgccgatcag ccctacaccg atgagggcaa tatggttcaa
    14581 aatgggcatt tgtgtaaacg gttttcgcaa agtaccgtca tggtagccta tcggcggaat
    14641 atgccgcaag ttcggcagga aaaaggagaa gaaatggaca aaatcagagt tgccgccgtg
    14701 cagatggtgt cgggcgtgtc gccggaaacc aacgtcgctg ccatgaaacg cctggtcgca
    14761 cgggcggcgg agcagggtgt ggattgggtg ctgctgcccg aatattgggt gctgatgggc
    14821 gcaaacgata ccgacaaact cgcgcttgcc gagcctttgg gcggcggacg ctttcagacg
    14881 gcattgagcg aaacggcgaa agaatgcggc gtggtgctgt tcggcggaac cgtgccgctg
    14941 caaagccccg aggcgggcaa ggtcatgaat actttattgg tgtacgggcg cgacggcaaa
    15001 cggacggggc tgtaccacaa aatgcacctc ttcggctttt ccggtttggg cgaacgctat
    15061 gccgaagccg ataccatcag cgcagggcgg gaagtgccgc acttgtcggc agaaggcgtg
    15121 tcggtggcgg cgggcatttg ttacgatgtc cgctttcccg aatttttccg ccgccagctg
    15181 ccgtttgacg tattgatgct gcccgctgcg tttacgcaca cgacgggcaa ggcgcattgg
    15241 gagctgctgc tgcgcgcgcg tgccgtcgaa aaccaatgtt acgtcgtggc ggcggcacag
    15301 ggcggtttgc acgaaaacgg acggcgcacg ttcggacaca gcatgattgt cgatccgtgg
    15361 ggcgacgtat tggacgtatt acccgaaggc gaaggcatcg ttacggcaga catcgatgcc
    15421 aaccgcctga acagcgtccg caaccgcctg cccgccttga aataccgggt tttggatgcc
    15481 gtctgaaggt tcagacggca tcggtgccgg ggaatcagcg ggctgtagga tgggcttcag
    15541 cctgccattt ccatcaatcc cacatttcta ccgttttcat cgaatccatc gaatccgccc
    15601 ctttcgacga cccagtcc
//
LOCUS       DAWKST010000035        15528 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.035, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000035 DAWKST010000000
VERSION     DAWKST010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15528
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.035"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            6..81
                     /locus_tag="WJL87_000756"
     tRNA            6..81
                     /locus_tag="WJL87_000756"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Trp,seq:cca)
     gene            196..474
                     /gene="secE"
                     /locus_tag="WJL87_000757"
     CDS             196..474
                     /gene="secE"
                     /locus_tag="WJL87_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ7799216.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            476..1012
                     /gene="nusG"
                     /locus_tag="WJL87_000758"
     CDS             476..1012
                     /gene="nusG"
                     /locus_tag="WJL87_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ7799217.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1113..1547
                     /gene="rplK"
                     /locus_tag="WJL87_000759"
     CDS             1113..1547
                     /gene="rplK"
                     /locus_tag="WJL87_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ7799218.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKEPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1547..2242
                     /gene="rplA"
                     /locus_tag="WJL87_000760"
     CDS             1547..2242
                     /gene="rplA"
                     /locus_tag="WJL87_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ7799219.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            2472..2972
                     /gene="rplJ"
                     /locus_tag="WJL87_000761"
     CDS             2472..2972
                     /gene="rplJ"
                     /locus_tag="WJL87_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ7799220.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3030..3401
                     /gene="rplL"
                     /locus_tag="WJL87_000762"
     CDS             3030..3401
                     /gene="rplL"
                     /locus_tag="WJL87_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ7799221.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAVDAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            3592..7770
                     /gene="rpoB"
                     /locus_tag="WJL87_000763"
     CDS             3592..7770
                     /gene="rpoB"
                     /locus_tag="WJL87_000763"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ7799222.1"
                     /translation="MSYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAAEKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNRLYNGSGKKEDLDSLTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSEDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            7927..12102
                     /gene="rpoC"
                     /locus_tag="WJL87_000764"
     CDS             7927..12102
                     /gene="rpoC"
                     /locus_tag="WJL87_000764"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ7799223.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKVALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRERILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIITDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQE
                     VEQETAETQVTDE"
     gene            12360..12731
                     /gene="rpsL"
                     /locus_tag="WJL87_000765"
     CDS             12360..12731
                     /gene="rpsL"
                     /locus_tag="WJL87_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ7799224.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            12849..13319
                     /gene="rpsG"
                     /locus_tag="WJL87_000766"
     CDS             12849..13319
                     /gene="rpsG"
                     /locus_tag="WJL87_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ7799225.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            13338..15443
                     /gene="fusA"
                     /locus_tag="WJL87_000767"
     CDS             13338..15443
                     /gene="fusA"
                     /locus_tag="WJL87_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ7799226.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
BASE COUNT         4146 a         3445 c         4010 g         3927 t
ORIGIN      
        1 tttagaggcc aatagctcaa ttggtagagt atcggtctcc aaaaccgagg gttgggggtt
       61 cgagaccctc ttggcctgcc aaataaaaaa ttaaccggcc ttgtgccggt taattttttt
      121 gtatttgtta tttagtaaac tctcttgcca tttacatgga ttgagaatag acagatgcta
      181 tgatggataa ataatatgac agaacatacg cctgaaaaaa agaacgttaa agtggatcaa
      241 ctggttgttc aagataaaga atctgcatct aattccggta aggaagggtt ttttgcatat
      301 ttctcaaatt cttggtccga attcaaaaag gtggtttggc ctaagcgtga agatgctgtc
      361 agaatgactg tatttgttat agtgtttgtt gctgtgcttt ctatatttat ctatgcggca
      421 gatacagcaa tttcgtggtt attttttgat gtattgctga gaagggaagg ttgagatgtc
      481 gaaaaaatgg tatgttgtac aggcgtattc ggggtttgag aagaatgtcc aacgaatatt
      541 ggaagagcgt attgcccgtg aggagatggg agattatttc ggacaaattc tggtgcctgt
      601 agagaaagtt gttgatatcc gcaatggtcg taagactatt agtgaaagaa agtcatatcc
      661 tggttatgtg ctagttgaga tggaaatgac agatgactct tggcatcttg taaaaagcac
      721 cccccgtgtt tccggtttta ttggagggag ggctaataga cctacgccga ttagtcagag
      781 agaggctgaa attattttac agcaggttca gaccggcata gagaagccga aaccaaaagt
      841 tgaatttgag gtcggtcaac aggttcgtgt aaatgaaggg ccgtttgcgg attttaacgg
      901 ggtggttgag gaggtcaatt atgaacggaa taagttacgc gtgtctgttc agatatttgg
      961 tagagaaaca cccgttgagc tggagttcag ccaggttgaa aagattaact gatttttata
     1021 cttgaaaaaa aagcaataag aggatagaat caaaaattaa cttggggagc ggaaatggtt
     1081 ccgcgtctta cccgttttta ggagtccgtt aagtggcaaa gaaaattatc ggctatatta
     1141 aactgcaaat tcctgcaggt aaagccaatc catctcctcc ggttggtcct gctttgggtc
     1201 agcgcggttt gaatattatg gaattttgta aggcatttaa tgctgcaacc caaggtatgg
     1261 agcctggctt accgattccg gttgtgatta ctgcatttgc agataaatca ttcacatttg
     1321 tgatgaaaac cccgccagct tctatcttgt tgaaaaaggc tgccggtttg caaaaaggta
     1381 gttctaatcc tctgaccaac aaagtgggta aattgacccg tgcccagttg gaagaaattg
     1441 ctaaaactaa agagcctgat ttgactgctg ctgacttgga tgcggctgtc cgtactatag
     1501 caggttctgc tcgctcaatg ggcttggatg tggagggtgt tgtataatgg ctaaagtatc
     1561 taaacgcttg aaagctcttc gctcttctgt ggaagccaat aaattatatg caattgatga
     1621 agcaattgct ttggtaaaaa aagcagcgac tgctaaattt gacgagtctg ttgacgtatc
     1681 tttcaacttg ggcgttgatc cgcgtaaatc tgaccaagtt atccgtggtt cggtcgttct
     1741 gcctaaaggc accggtaaga taacccgtgt ggctgtattt actcaaggtg caaatgcaga
     1801 agctgctaaa gaagctggtg cagatatcgt cggtttcgaa gatttggctg ctgaaatcaa
     1861 agcaggcaat ctgaactttg acgttgttat tgcttctccc gatgcaatgc gtattgttgg
     1921 tcaattgggt actattttgg gtcctcgagg cttgatgcca aaccctaaag taggtactgt
     1981 tactcctaac gttgctgaag cagtaaaaaa tgcaaaagca ggtcaagtgc agtaccgtac
     2041 cgacaaggca ggtattgttc atgcaacgat tggtcgtgct tcattcgctg aagctgattt
     2101 gaaagagaac tttgatgcgt tgctggatgc tatcgttaaa gccaagcctg ctgccgctaa
     2161 aggtcagtat ctgaaaaaag ttgctgtgtc tagcaccatg ggtttgggta ttcgcgttga
     2221 tacatcaagc gtaaataact aatcttaagg aattttcaag cagtttggtt ttctgggctg
     2281 cttgaatttg ggctacttaa aattaagtag atgtccaaga ccgtagggat cgtaagattt
     2341 aatcgtaact gccctacgca gacggtagtc ctgaaacaca ttgcaagatt gcttgtaaga
     2401 tgtcttttta ggttaccgcg ctggtgggat atcgttttgg tatcctgttt ataaacagtg
     2461 ggaggtagac cttgagtctc aatattgaaa ccaagaaagt ggcggtcgag gaaattagcg
     2521 cggcaattgc taatgctcaa accctcgtag tcgctgaata tcgcggtatc agtgtttcca
     2581 gtatgactga gcttcgtgcg aatgcacgta aagaaggcgt ttatttgcgc gttctgaaaa
     2641 atactttggc tcgtcgtgca gtgcaaggta cttcatttgc agaattggcc gatcaaatgg
     2701 ttggtccgtt ggtttacgct gcttctgaag atgctgttgc tgctgctaaa gtgttgcacc
     2761 aattcgcgaa aaaagatgac aaaattgtcg ttaaagccgg ttcttacaat ggcgaagtaa
     2821 tgaatgctgc tcaggttgct gagttggctt ctattccgag ccgcgaagag ctgttgtcca
     2881 aactgttgtt cgttatgcaa gctcctgtat cgggctttgc gcgcggtttg gctgctttgg
     2941 cagagaaaaa agccggcgaa gaagccgctt aatcgatttt gtttctgtta atcaattatt
     3001 ttttaataca atatttggag taaaatagca tggctattac taaagaagac attttggaag
     3061 cagttggttc tttgaccgta atggaattga acgacttggt taaagctttt gaagaaaaat
     3121 tcggtgtttc tgctgctgct gttgcagttg caggtcctgc tggtgccggt gctgtcgatg
     3181 ctgaagaaaa aaccgaattt gatgtcgttt tggcttctgc cggcgatcaa aaagtcggcg
     3241 tgattaaagt tgtccgtgca attaccggtt tgggtctgaa agaagctaaa gacatcgttg
     3301 acggtgcacc taaaaccatt aaagagggtg tttctaaagc tgaagccgaa gacatccaaa
     3361 aacaactgga agaagccggc gctaaagtcg aaatcaaata atttgatgct tcttatgaag
     3421 gctggcagtt ttctgccagc cttattttgc ttcttaaaat aaacatcaag tattgtttac
     3481 atttatttgc atagttttta tcaaatcatt gcaaataaat gtaaacatca gattgatgcg
     3541 tgccgttgct ttcaaacggc ctattattga aaattacttt tcggagtgta tatgagctat
     3601 tcgtttaccg agaaaaaacg tatccgtaag agttttgcaa agcgggaaaa tgttttggaa
     3661 gttcctttct tgctagcaac ccaaattgat tcttatgcga agtttttgca gctggaaaat
     3721 gcttttgaca aacgtaccga tgacggtctg caggcagcat ttaattctat tttcccgatt
     3781 gtgagccata acggttatgc gcgattggag tttgtgcatt acacattggg cgagcctttg
     3841 ttcgatattc ccgaatgtca gttgcgcgga atcacttatg cagccccctt gcgcgcgcgt
     3901 atccgtttgg tgattttgga taaggaagca tctaaaccga cggtaaaaga agttcgtgaa
     3961 aacgaagtgt atatgggcga aattccgttg atgaccccga gcggttcttt tgtgattaac
     4021 ggcacagagc gtgtgattgt ctcccagttg caccgttcgc ccggcgtatt cttcgagcat
     4081 gacaaaggta agacgcactc ttccggcaaa ttgttattct ccgcccgcat cattccctac
     4141 cgtggttcat ggttggattt tgaatttgat ccgaaagatt tgctgtattt ccgtatcgac
     4201 cgccgccgta aaatgccggt aacgattttg ttgaaggctt tgggctacaa caatgagcaa
     4261 atcttggata ttttctacga caaagaaacg ttctatttgt cttcaaacgg tgttcaaacc
     4321 gatttggtcg caggccgtct gaaaggcgaa actgccaagg tcgatatctt ggataaagaa
     4381 ggcaatgtat tggttgccaa aggtaagcgt attactgcga aaaatatccg tgatattacc
     4441 aatgcaggcc tgacccgttt ggatgtagaa ccggaaagcc tgctgggcaa agcattggct
     4501 gccgatctga ttgattcgga aaccggcgag gtattggctt ctgccaatga tgaaattaca
     4561 gaagagttgt tggccaaatt tgatatcaac ggcgtaaaag aaattacgac cctttacatc
     4621 aatgagttgg atcagggtgc ttatatctcc aataccttgc gcacggatga gactgccggc
     4681 cggcaggcgg cgcgtgttgc gatttaccgt atgatgcgtc cgggcgaacc gcccaccgaa
     4741 gaggcagtcg agcaattgtt taaccgcttg ttcttcagtg aagacagcta cgatttgtcc
     4801 cgcgtaggtc gtatgaaatt taatacgcgc acatacgaac aaaaactgtc cgaagcccaa
     4861 caaaactctt ggtacggccg cctgctgaac gaaacgtttg ccggtgctgc cgaaaaaggc
     4921 ggttatgtcc tgagcgtcga agatattgtc gcctcgattg cgactttggt cgagttgcgt
     4981 aacggccatg gtgaagttga cgatatcgat cacttgggca accgccgagt acgttcggta
     5041 ggcgagctga ctgaaaacca attccgcagc ggtttggccc gtgtggaacg tgctgtaaaa
     5101 gaacgtttga atcaggcgga atcagaaaac ttgatgccgc acgatttgat taatgcaaaa
     5161 cctgtttctg ccgctattaa agaattcttc ggctccagcc aattgagtca gtttatggat
     5221 cagaccaacc ccttgtctga agtaacccat aaacgccgcg tatctgcatt gggtccgggc
     5281 ggtttgaccc gcgaacgtgc cggcttcgag gtacgggacg tgcatccgac ccactacggc
     5341 cgcgtatgtc cgattgaaac gcctgaaggt ccgaacatcg gtttgatcaa ctcattgtcc
     5401 gtttatgcgc gcaccaatga ttacggtttc ttggaaacgc cttaccgccg cgttatcgac
     5461 ggcaaagtaa ccgaggaaat cgattacttg tctgccatcg aagaaggccg ctatgtgatt
     5521 gcacaggcga atgccgattt ggattcagat ggcaatctga ttggcgattt ggttacctgt
     5581 cgtgaaaaag gcgaaaccat tatggcaacg cccgaccgcg tccaatatat ggacgtggca
     5641 accggccagg tggtatccgt tgcggcatcc ctgattccgt tcttggaaca tgatgacgcg
     5701 aaccgcgcat tgatgggtgc caacatgcaa cgtcaggcag tgccttgctt gcgtcctgaa
     5761 aaaccgatgg tcggtaccgg tatcgagcgt tccgttgccg ttgactctgc tactgcaatc
     5821 gttgcccgcc gaggcggcgt ggtcgagtat gtcgatgcca accgtgttgt gattcgtgtc
     5881 catgacgacg aagcgactgc cggcgaagtg ggtgtcgata tttacaactt ggttaaattt
     5941 acccgttcca accagtctac caatatcaat cagcgtcctg ccgtcaaagc cggcgatgtt
     6001 ttgcaacgcg gcgatttggt ggccgatggc gcgtccaccg atttgggcga attggctttg
     6061 ggtcaaaata tgaccatcgc cttcatgccg tggaacggtt acaactacga agactcgatt
     6121 ctgatttccg aaaaagtggc tgcggatgat cgttacactt cgattcacat tgaggaattg
     6181 aatgtcgttg cccgcgatac caagctgggt gcggaagaca ttacccgcga tattccgaac
     6241 ttgtccgagc gtatgcaaaa ccgcttggac gaatccggta tcgtttacat cggtgcagaa
     6301 gtagaagccg gcgatgtgtt ggtaggcaaa gtaacgccta aaggcgaaac ccaactgacg
     6361 ccggaagaaa aactgttgcg cgccatcttc ggcgaaaaag cgtctgacgt aaaagatact
     6421 tcactgcgta tgcctaccgg tatgagcggt acggttatcg acgttcaagt cttcactcgt
     6481 gaaggtattc aacgcgacaa acgcgcccaa tccattatcg attccgagtt gaaacgctac
     6541 cgtttggatt tgaacgacca attgcgtatt ttcgacaacg acgcattcga ccgtatcgaa
     6601 cgtatgattg tgggtcaaaa agccaatggt ggtccgatga agctggctaa aggcagcgaa
     6661 attactaccg aatatttggc gggtctgccg agcaggcacg attggttcga tatccgtctg
     6721 accgatgaag atttggccaa gcagttggaa ctgattaaag tgagcctgca acaaaaacgc
     6781 gaagaagcgg acgagttata cgaaatcaag aagaaaaaac tgacccaagg cgacgaattg
     6841 caacccggcg tacaaaaaat ggtgaaagtt tttatcgcca tcaaacgccg tctgcaagcc
     6901 ggcgacaaaa tggcgggccg ccacggtaac aaaggcgtgg tatcgcgcat tctgccagtg
     6961 gaagacatgc cttacatggc ggacggccgt ccggtagaca tcgtactgaa cccattgggc
     7021 gtaccttccc gtatgaacat cggtcagatt ttggaagttc acttgggttg ggcagcaaaa
     7081 ggtatcggcg agcgtatcga ccgtatgctg aaagagcaac gcaaagcagg cgagttgcgc
     7141 gagttcttga acagactcta caacggcagc ggtaagaaag aagatttgga cagcctgaca
     7201 gatgaagaaa tcatcgaatt ggcttccaac ctgcgtaaag gtgcatcttt cgcttctcct
     7261 gtattcgacg gtgcgaaaga gtctgaaatc cgcgaaatgt tgaacttggc ttacccaagc
     7321 gaagatcctg aggttgagaa actgggcttc aacgacagta aaactcaaat cacgctgtat
     7381 gacggccgtt caggcgaagc atttgatcgc aaggttacag tcggtgtgat gcactatctg
     7441 aaactgcacc acttggttga cgaaaaaatg cacgcccgtt ctaccggtcc atacagtctg
     7501 gttacccaac agcctttggg cggtaaagct cagttcggtg gccaacgttt cggtgagatg
     7561 gaggtttggg cattggaagc atacggtgcg gcatacacgc tgcaagagat gctgactgtg
     7621 aagtctgatg acgtgaacgg ccgtaccaaa atgtacgaga acatcgtcaa aggcgaacac
     7681 aaaatcgatg ccggtatgcc cgagtccttc aacgtattgg ttaaagagat tcgttcactg
     7741 ggcttggata tcgatttgga acgctactaa gcagaagttt tcagacggcc tttcaaggtc
     7801 gtctgaaaaa gcggtttcag aataagaatg aagcaatcgg catttaggcc gtctgaaatc
     7861 gaaagtaccg tttcccaata tcgaaaatcc gccacgcggt aaaaatactt ccttcaagga
     7921 gcaaaaatga atttgttgaa cttatttaat ccgttgcaaa ctgccggcat ggaagaagag
     7981 tttgatgcca tcaaaatcgg cattgcttct cccgaaacca tccgttcatg gtcttacggc
     8041 gaagttaaaa aacctgaaac catcaactac cgtacgttca aacctgagcg tgacggtctg
     8101 ttctgcgcca aaatctttgg tccggtcaaa gactacgaat gcttgtgcgg aaaatacaaa
     8161 cgcttgaaat ttaaaggcgt aacctgtgaa aaatgcggcg tagaagtgac cctgtccaaa
     8221 gtgcgccgcg aacgcatggg ccacattgaa ttggcagcac ccgttgccca tatttggttc
     8281 ttgaaatccc tgccttcccg cttgggtatg gtactggaca tgactttgcg cgacatcgaa
     8341 cgcgtattgt actttgaagc atttgtggta accgaccccg gtatgactcc gctgcaacgc
     8401 cgccaattgc tgactgaaga cgattactac aacaaactgg acgaatacgg cgatgacttc
     8461 gatgccaaaa tgggtgcgga aggtatccgc gaattgctgc gtaccttgaa tgtggcgggc
     8521 gaaatcgaaa tcctgcgcca agagttggaa tcgaccgggt ccgacaccaa aatcaaaaaa
     8581 atcgccaaac gtttgaaagt attggaagcc ttccaccgtt ccggtatgaa gctggaatgg
     8641 atgattatgg atgtgctgcc ggtattgccg cctgatttgc gtccgttggt tccattggat
     8701 ggcggtcgtt ttgccacttc cgatttgaac gatttgtacc gtcgcgtcat caaccgtaac
     8761 aaccgtctga aacgtctgtt ggaactgcat gcgcccgaca ttatcgtccg caacgaaaaa
     8821 cgtatgttgc aagaagcggt cgactctctg ttggataacg gccgtcgcgg taaagccatg
     8881 accggcgcca acaaacgccc gctgaaatca ttggcagaca tgattaaagg taaaggcggt
     8941 cgcttccgtc aaaacctgtt gggcaaacgt gtggactact ccggccgttc cgtgattacc
     9001 gtaggcccat acctgcgtct gcaccaatgc ggtctgccga aaaaaatggc tttggaactg
     9061 ttcaaaccgt tcattttcca caaattggaa aaacaaggtt tggcctctac cgttaaagca
     9121 gcgaaaaaat tggtagagca agaagtaccg gaagtatggg acatcttgga agaagtcatc
     9181 cgcgaacatc cgattatgct gaaccgtgcg ccgaccctgc accgtttggg tattcaagcg
     9241 ttcgaaccta tcttgattga aggtaaagcg attcagttgc acccattggt gtgtgctgcg
     9301 ttcaacgccg actttgacgg cgaccaaatg gcggtacacg ttccattgag cttggaagca
     9361 caaatggaag cacgcacgct gatgctggct tcaaacaacg tattgtctcc tgccaacggc
     9421 gaaccgatta tcgtaccttc ccaagacatc gtattgggcc tgtactacat gacccgcgac
     9481 cgtatcaatg ccaaaggcga gggcagcctg tttgccgatg tgaaagaagt gcatcgcgca
     9541 taccatacca aacaggttga actgggtacg aaaattaccg tacgtctgcg cgaatgggtg
     9601 aaaaacgaag caggtgagtt tgagcctgtc gttaaccgtt acgaaacaac cgtcggccgt
     9661 gcattgttga gcgaaatcct gccgaaaggc ctgccgtttg aatatgtcaa caaagcgttg
     9721 aagaaaaaag aaatttctaa actgattaac gcatcgttcc gcctgtgcgg cttgcgcgat
     9781 acggttatct ttgctgacca cctgatgtac accggtttcg gatttgcggc aaaaggcggt
     9841 atttccattg ccgttgacga tatggaaatt ccaaaagaaa aagtggcctt gctggctgaa
     9901 gccaatgccg aggttaaaga aatcgaagac caataccgtc aaggtttggt taccaacggc
     9961 gaacgctaca acaaggtggt cgatatttgg ggtcgtgccg gcgataagat tgctaaagcg
    10021 atgatggaca acttgtccaa acaaaaagtt atcgaccgtg acggcaacga agtcgatcaa
    10081 gagtcattca actccattta tatgatggcg gactccggtg cccgtggttc tgcagctcag
    10141 attaaacagt tgtccggtat gcgtggcttg atggcaaaac ctgacggctc gattattgaa
    10201 acgccgatta cctcaaactt ccgtgaaggt ctgaccgtat tgcaatactt tattgcgacc
    10261 cacggtgcgc gtaagggttt ggcggatacc gcattgaaaa ccgcgaactc cggttacctg
    10321 acccgtcgtc tggtagacgt aactcaagat ttggtcgttg ttgaagacga ttgcggtact
    10381 tcagacggct ttgtcatgaa ggcagtggta caaggcggtg atgtgattga agcattgcgc
    10441 gagcgtattt tgggccgtgt tactgcgtct gacgttgtcg atccgtcaag cggcgagacc
    10501 ttggttgaag caggcacgtt gttgactgaa aaactggtgg atatgattga ccaatccggt
    10561 gtcgatgaag tcaaagtccg tactccgatt acttgtaaaa cccgccacgg tttgtgcgcg
    10621 cactgttacg gccgcgactt ggcgcgcggc aaactggtca atgccggaga ggcagtcggc
    10681 gtgattgccg cccaatcaat cggcgaaccg ggtacccagc tgactatgcg tacgttccac
    10741 atcggtggtg cggcatcccg tgcggcagca gccagccaag tggaagccaa atccaacggt
    10801 acggcacgat tcagcagcca aatgcgttat gttgccaaca ataaaggcga gttggttgtc
    10861 atcggccgtt cttgtgaagt cgtgattcac gatgacatcg gccgtgaacg cgaacgccac
    10921 aaagtacctt acggtgccat cctgctggta caagacggta tggccattaa agccggtcaa
    10981 accttggcaa cctgggatcc gcatacccgt ccgatgatta ccgaacacgc aggtatggtg
    11041 aaattcgaaa acgtggaaga gggcgttacc gttgccaaac aaaccgatga tgtaaccggt
    11101 ttgtccactt tggtagtgat cgacggtaaa cgtcgttcct ctagtgcttc caaactgctg
    11161 cgtccgactg tgaaactctt ggacgaaaac ggcgtggaaa tctgtattcc cggtacttct
    11221 actccggtat ccatggcatt ccccgttggt gcggtgatta ccgtacgcga aggtcaggaa
    11281 atcggtaaag gcgacgtatt ggcgcgtatc ccgcaagcct cttccaaaac ccgcgacatt
    11341 accggtggtc tgccgcgcgt tgccgaattg tttgaagcac gcgtgccgaa agatgccggt
    11401 atgttggcgg aaattaccgg taccgtttcc ttcggcaaag agaccaaagg caagcaacgt
    11461 ctgattatta ccgacgtgga cggcgtagca tacgagactt tgatttccaa agagaaacag
    11521 attttggtac acgacggtca agtggtaaac cgcggcgaaa ccattgtgga cggcgcggtc
    11581 gatccgcacg atattctgcg tctgcaaggt atcgaagcac tggcacgcta cattgtccaa
    11641 gaggtgcaag aggtttaccg tctgcaaggt gtgaagattt ccgacaaaca catcgaagtc
    11701 atcatccgtc aaatgttgcg ccgtgtgaac attgcggatg ccggcgaaac cggcttcatt
    11761 accggcgagc aggtcgaacg cggcgatgtg atggctgcca atgaaaaagc tttggaagaa
    11821 ggcaaagaac cggcacgtta cgaaaacgta ttgctgggta ttaccaaagc ctccctgtct
    11881 accgacagct tcatttctgc cgcatcgttc caagaaacga cccgcgttct gaccgaagcc
    11941 gcgattatgg gcaaacaaga cgagctgcgc ggtctgaaag agaacgtgat tgtcggtcgt
    12001 ttgattcctg ctggtactgg tttgacttac caccgcagcc gtcatcaaca atggcaagag
    12061 gtggagcagg agactgctga aacccaagta acggatgaat aatctttggt gcgtttattc
    12121 aatacaaaac cgcaagcttt aggcttgtgg tttttctttc agacggcatt taaattatta
    12181 gtgatttcct ttccggtaat agttgtttat gagcataagg gacctaaatg tttatctgaa
    12241 aaaagaaaaa tgatgaatct tcctgatctc acgcttgact gtctgcttgc aataaaaata
    12301 taattccact cttgccgaca tggtgtcggc aagtatttaa ctcaacagga cgagaaaata
    12361 tgccaactat caaccaattg gtacgcaaag gccgtcaaaa gcccgtgtac gtaaacaaag
    12421 tgcccgcact ggaagcctgc ccgcaaaaac gcggcgtgtg cacccgtgta tacacgacta
    12481 cccctaaaaa acctaactct gcattgcgta aagtatgtaa agtccgcctg accaacggtt
    12541 ttgaagtcat ttcatacatc ggcggcgaag gccacaacct gcaagagcac agtgtcgtat
    12601 tgattcgcgg cggtcgtgta aaagacttgc caggtgtgcg ttaccacact gtacgcggtt
    12661 ctttggatac tgcaggcgtt aaagaccgta aacaagcccg ttccaaatac ggtgctaagc
    12721 gtcctaaata attactggga cttaaatagg cacgtcggcc gcctaagctg aacaacggcc
    12781 gagtaagtga atactcaatt gggtattcat gggaatagac ccgactgaat agattaaagg
    12841 aaattaaaat gccaagacgt agagaagtcc ccaagcgcga cgtactgcca gatcctaaat
    12901 tcggcagcgt cgagttgacc aaattcatga acgtattgat gattgacggt aaaaaatccg
    12961 ttgccgagcg tatcgtttac ggtgcgttgg aacagattga gaaaaaaacc ggcaaagtag
    13021 caatcgaagt atttaacgaa gccattgcaa acgccaagcc tatcgtggaa gtgaaaagcc
    13081 gccgtgtagg tggtgcaaac taccaagttc ctgttgaagt tcgtccttca cgccgtttgg
    13141 ctttggcaat gcgctgggtt cgcgatgcgg cccgcaaacg tggtgagaaa tccatggacc
    13201 tgcgtttggc aggcgaattg attgatgcgt ccgaaggtcg tggcggtgcg ttgaaaaaac
    13261 gtgaagaagt acaccgtatg gctgaagcca acaaagcatt ctctcacttc cgtttctaat
    13321 tttgaaaggc taataaaatg gctcgtaaga ccccgatcag cctgtaccgt aacatcggta
    13381 tttccgccca tatcgatgcg ggtaaaacca cgacgacaga acgtattttg ttctatactg
    13441 gtttgaccca caagctgggc gaagtgcatg acggtgcggc tactaccgac tacatggaac
    13501 aagagcaaga gcgcggtatt accattacct ccgctgccgt tacttcctac tggtccggta
    13561 tggcgaaaca attccccgag caccgcttca acatcatcga caccccggga cacgttgact
    13621 ttaccgtaga ggtagagcgt tctatgcgtg tattggacgg cgcggtaatg gtttactgcg
    13681 cggtgggcgg tgttcaaccc caatctgaaa ccgtatggcg gcaagccaac aaataccaag
    13741 tgccgcgctt ggcgtttgtc aataaaatgg accgtcaggg tgccaacttc ttccgcgttg
    13801 tcgagcaaat gaaaacccgt ttgcgcgcaa accctgtacc tatcgtcatt ccggttggtg
    13861 cggaagacaa cttcagcggt gtggttgatt tgttgaaaat gaaatccatc atttggaatg
    13921 aagccgataa aggtacaacc tttacctatg gcgatattcc tgccgaattg gtcgaaactg
    13981 ccgaagaatg gcgtcaaaat atgattgaag ccgcagccga agccagcgaa gaactgatgg
    14041 acaaatactt aggcggcgac gagctgaccg aagaagaaat cgtaggcgcg ttgcgtcaac
    14101 gtactttggc aggcgaaatt cagcctatgc tgtgtggttc tgcatttaaa aacaaaggtg
    14161 ttcaacgtat gttggacgca gttgtagaat tgctgccagc tcctaccgat attcctccgg
    14221 ttcaaggtgt caacccgaat accgaggaag ccgacagccg tcaagccagc gatgaagaga
    14281 aattctctgc attggcgttc aaaatgttga acgacaaata cgtcggtcag ctgaccttta
    14341 tccgcgttta ctcaggcgta gtaaaatccg gcgataccgt attgaactcc gtaaaaggca
    14401 ctcgcgaacg tatcggtcgt ttggtacaaa tgactgccgc agaccgtact gaaatcgaag
    14461 aagtacgcgc cggcgacatc gcagccgcta ttggtctgaa agacgttact accggtgaaa
    14521 ccttgtgtgc ggaaagcgcg ccgattatct tggaacgtat ggaattcccc gagccggtaa
    14581 tccatattgc cgttgagccg aaaaccaaag ccgaccaaga gaaaatgggt atcgccctga
    14641 accgcttggc taaagaagac ccttctttcc gtgtccgtac agacgaagaa tccggtcaaa
    14701 ccattatttc cggtatgggt gagctgcact tggaaattat tgttgaccgt atgaaacgcg
    14761 aattcggtgt ggaagcaaat atcggtgcac ctcaagtggc ttaccgtgaa actatccgca
    14821 aagccgttaa agctgaatac aaacacgcga aacaatccgg tggtaaaggt caatatggtc
    14881 acgttgtgat tgaaatggaa cctatggaac cggggggcga aggttacgag tttatcgatg
    14941 aaattaaagg tggcgtgatt cctcgcgaat tcattccgtc tgtcgataaa ggtatccgcg
    15001 atacattgcc taacggtatc gttgccggct atcctgtagt tgatgtacgt atccgtctgg
    15061 tattcggttc ttaccatgat gtcgactctt cccaattggc atttgaattg gccgcttctc
    15121 aagcgtttaa agaaggtatg cgtcaagcat ctcctgccct gcttgagccg attatggcag
    15181 ttgaagtgga aactccggaa gaatacatgg gcgacgtaat gggcgacttg aaccgccgtc
    15241 gcggtgttgt attgggtatg gatgatgacg gtatcggcgg taaaaaagtc cgtgccgaag
    15301 tacctctggc agaaatgttc ggttattcga ccgacctgcg ttctgcaacc caaggccgcg
    15361 ctacttactc tatggagttc aagaaatatt ctgaagctcc tgcccacata gctgctgctg
    15421 taactgaagc ccgtaaaggc taatcaggca aataggtcgt ctgaaaggct gaaatgattt
    15481 ttcagacggc attgttcttt aatcgatctt taatgtaaag gaattagc
//
LOCUS       DAWKST010000036        15225 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.036, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000036 DAWKST010000000
VERSION     DAWKST010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15225
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.036"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..139)
                     /locus_tag="WJL87_000768"
     CDS             complement(<1..139)
                     /locus_tag="WJL87_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ7799227.1"
                     /translation="MSVQTVTYNKFFMNTNQPAVYDPLTRALHWLTVAGFIGILTTIV
                     LW"
     gene            381..1535
                     /gene="obgE"
                     /locus_tag="WJL87_000769"
     CDS             381..1535
                     /gene="obgE"
                     /locus_tag="WJL87_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ7799228.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGEARSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQTRTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            1618..2667
                     /locus_tag="WJL87_000770"
     CDS             1618..2667
                     /locus_tag="WJL87_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ7799229.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDACMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3355..4776
                     /locus_tag="WJL87_000771"
     CDS             3355..4776
                     /locus_tag="WJL87_000771"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ7799230.1"
                     /translation="MTTIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGASGHTCGHDHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPPADPMPSEADDDYTRRFYVAMNDDFDTVKAVAVLFELAGEVNKTNDAQL
                     AGCLKALGGIIGLLQRDPTEFLQGGAASDGLSNEEIEDLIARRKQARSDKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            4859..5638
                     /gene="xth"
                     /locus_tag="WJL87_000772"
     CDS             4859..5638
                     /gene="xth"
                     /locus_tag="WJL87_000772"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ7799231.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVMEYDYAAE"
     gene            5625..5969
                     /locus_tag="WJL87_000773"
     CDS             5625..5969
                     /locus_tag="WJL87_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799232.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPTWALVWLLICMPCLLIASKCLGWKGWRRVVNIFACLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            5980..6510
                     /locus_tag="WJL87_000774"
     CDS             5980..6510
                     /locus_tag="WJL87_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ7799233.1"
                     /translation="MQGFELEDLTLWLIRDAGEDQMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLRTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVPEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(6603..7718)
                     /gene="asd"
                     /locus_tag="WJL87_000775"
     CDS             complement(6603..7718)
                     /gene="asd"
                     /locus_tag="WJL87_000775"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ7799234.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANNVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWIDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGAETNKILGRSDNPTVIDGLCVRIGSMRCHS
                     QALTLKLKKDLPISEIEAILAGANDWVKVIPNEKEASIRELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
     gene            7894..8475
                     /locus_tag="WJL87_000776"
     CDS             7894..8475
                     /locus_tag="WJL87_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ7799235.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(8537..9391)
                     /gene="folD"
                     /locus_tag="WJL87_000777"
     CDS             complement(8537..9391)
                     /gene="folD"
                     /locus_tag="WJL87_000777"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ7799236.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQNNLHTPCLAVVLVGGD
                     PASAVYVRNKKTSCQKCGIKSLSYELPESTSQEELLALVDRLNVDSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLADEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            9614..9691
                     /locus_tag="WJL87_000778"
     tRNA            9614..9691
                     /locus_tag="WJL87_000778"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9649..9651,aa:Pro,seq:tgg)
     gene            9716..9792
                     /locus_tag="WJL87_000779"
     tRNA            9716..9792
                     /locus_tag="WJL87_000779"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9750..9752,aa:Arg,seq:tct)
     gene            9820..9895
                     /locus_tag="WJL87_000780"
     tRNA            9820..9895
                     /locus_tag="WJL87_000780"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9853..9855,aa:His,seq:gtg)
     gene            10031..10537
                     /locus_tag="WJL87_000781"
     CDS             10031..10537
                     /locus_tag="WJL87_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HEZ7799237.1"
                     /translation="MVDAWSVPDFESKICPPEALSERLARLPRPLVFTNGCFDILHRG
                     HVTYLAQARSAGAALVLALNTDASVRRLGKDGDRPINPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            10534..12312
                     /locus_tag="WJL87_000782"
     CDS             10534..12312
                     /locus_tag="WJL87_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ7799238.1"
                     /translation="MTVLKLSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLDVQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRSDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKVDGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            12425..13249
                     /locus_tag="WJL87_000783"
     CDS             12425..13249
                     /locus_tag="WJL87_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ7799239.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(14012..14800)
                     /locus_tag="WJL87_000784"
     CDS             complement(14012..14800)
                     /locus_tag="WJL87_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ7799240.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSIQTAQPAMITVSLRR
                     AGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
     gene            complement(15014..15208)
                     /gene="thiS"
                     /locus_tag="WJL87_000785"
     CDS             complement(15014..15208)
                     /gene="thiS"
                     /locus_tag="WJL87_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEZ7799241.1"
                     /translation="MNIILNGGPAELHGTSVADLIAQTAPQQPFAVAVNTVFIPKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT         3630 a         3848 c         4232 g         3515 t
ORIGIN      
        1 tccacaggac aatggtggtc agaatgccga tgaaaccggc aacggtcagc cagtgcagcg
       61 cgcgtgtcaa cgggtcgtaa acggcaggtt ggtttgtgtt cataaaaaac ttattatatg
      121 taacggtttg aacggacatt ccgtcgggtc ggaatgtcaa aaaggcgcga ttgtaccaaa
      181 gaagttggac ataattttgt ttgcaggctg aagatttgct taaaaattca ttaagatggg
      241 cggaacaaat agtttgggta cggcgtgttg aaataccgct tatcccttat aatagcgtcc
      301 gaaattctgt tcagacggca tcaaaacaca cggtaatccg tctgaacccc ccatttgaaa
      361 ccaacaaaca agagcattga atgaaattca tcgacgaagc aaaaatcgaa gtcgccgcag
      421 gcaaaggcgg taatggcgca accagtttcc gccgcgaaaa attcgtaccg cgcggcggcc
      481 cggacggcgg cgacggcggc aaaggcggta gcgtctgggc ggaagccgac gaaaacacca
      541 acaccctcgt cgaataccgc ttcgttaaac gctaccaagc caaaaacggc gaaaaaggcc
      601 acggttccga ccgttacggc gcaggtgcgg acgacatcgt cctcaaaatg cccgtcggca
      661 ccctcatccg cgacctcgac accgacgaaa tcgttgccga cctcacttac cacggacagc
      721 gcgtctgcct agccaaaggc ggcaaaggcg gcttgggcaa catccacttc aaatcctccg
      781 tcaaccgcgc cccaaaacaa tccacgcccg gcgaagaagg cgaagcccgt tccctgcaac
      841 tcgaactcaa agtcctcgcc gatgtcggct tattgggtat gcccaacgcc ggcaaatcca
      901 ccctgattac cgccgtatcc gccgcacgcc ccaaaatcgc caactacccc ttcaccaccc
      961 tgcatccaaa cttaggcgtg gtacgcatcg acgaaaacca cagcttcgtg atggccgaca
     1021 tccccggcct gattgaaggc gcggcagaag gcgcgggttt ggggcatcgt ttcctcaaac
     1081 acttatcgcg caccggatta ttgctgcacg tcgtcgattt ggcgcccttc gacgaaaccg
     1141 tcaaccctgc cgaagaagca cttgctatca tcaacgaatt gcgcaaatac gatgaagaac
     1201 tttacggcaa accgcgctgg ctggtgctga ataaactcga catgcttgac gaagaagaag
     1261 cccaaacgcg cactgccgcc ttcctcgaag ccgtcggctg ggactacccc aaacccgacg
     1321 accgtttcca atttgacatg gaaaccccgc gcctcttcca aatcagcgcg ctgacccacc
     1381 aaggcacgca ggaattggta caccaaatca accaatacct gaccgagaaa aaacgtatcg
     1441 aagctgaaaa agccgaagcg gagaaagcag cggcaaatgt tgaaattatc gaacagcagc
     1501 ctaaaacgga tacgggcgtg tttaagccgg agtgaaaaaa gaaagggttt cagacgatat
     1561 ttgttctaca aaaggttgtt tgaaacaatt ttaaagcaag attgaaatgg attaaaaatg
     1621 tcatctaacc aatatatcca gtcgcttgag ttgacaaact ttaccgttct tccaaacgat
     1681 acttttgaat tttctgaaaa tctcaatgtt attgttgccg aaaacggttg tggcaaaaca
     1741 catttgttaa aaattttata cagcctgcta gaagtaacat ctaacaccaa aaacagatta
     1801 ctgaaaacag aattgcaaaa aagttttgcc gataaattac tgaatgtttt ccgtcccgat
     1861 tccttgggca ggttgagcaa acgtttgcag gggcgcgggc gtacggaaat cgttttaaaa
     1921 ctgcaaaacg gcactacgca tagtagtctg aatttttcca gtaactcatc atcgcaagtc
     1981 aatgttcaaa gcatcggatt gaaagaaaat gaatatactc cgacacctat cttcttaccc
     2041 agccgcgaat taattacact ttgcccttgg ttcacttctt tgtatcaaaa ccaaagcatc
     2101 cctttcgaag aaacttggtt tgatgcctgt atgcaattaa atcacccgct tgccaaaggc
     2161 cccagagaaa caaaaattcg agaattgctc gaacctattg agaacgcgat gggcggaaaa
     2221 gtatctgaag aacaaggaag gttttatcta tcgctgacaa atacgggcgg taagattgaa
     2281 gcacctttgg tagcagaggg tttgcggaaa tttgtcatga ttgcccggtt aatcgcaaca
     2341 ggggcattgc tggataaagg ctatctattt tgggatgaac cggaagctaa tctgaatcct
     2401 aaattgatca aagtggctgc ccgtattatt tggtctttat ctcaacaggg tattcaggtt
     2461 tttattgcaa cacactcttt attcttattg agagaattgg aacttttgaa gcttgaaaat
     2521 aacacggatt tacctgtacg ttttttctcg ttgattgctt ccgatgatgg aacaaaagtg
     2581 gagcaaggga acagtattaa tgatttgaac acattgatat tgttagatga aaacattatg
     2641 caggcagaca ggtatctgaa tatttgaggt taagctatga ctacaatcac agaaaagaga
     2701 ttaacctttg cttttcctga agattattac gttaccaagt atgacgaatg ggaacattat
     2761 aaaatttttc agaacagttg taatttacgt aataaaattg atacaaatga aaagggaaag
     2821 aacgggataa atcaaagtgt agatgacgat aatggcagct caggtgtaga tataatagcc
     2881 cttcatgagt cgacactttg gctaatcgaa attaaagatt attaccgact tgggcttgag
     2941 ccaaatgccc aatcaataga tgaaaaactt tctgacctac catatttaat cgcaagaaaa
     3001 attcgagatt cattggcagg attggtttct gctaaattta aagcagcaaa acaggaagaa
     3061 aaagattttt ctcgcttggc tttagattgt aacgaaataa aaatagtcct tcatattgaa
     3121 atgcctagta taagaagtaa actttatccc tcgtcatcag atttagcaaa cttattaaaa
     3181 gataagttta aattatcaga atttacaaaa aattttgcaa actgttatgc agaaccaatt
     3241 ttcacaaata tcagccatat taataatccg caactgcgta atgttccttg gtccgtatct
     3301 acaggaacag aacagaaatt atcatccgag caacaaagat taatccataa tcccatgacc
     3361 accatctaca acacccttac ccgccaaaaa gaaccctttg cccccatcga ccctgaaaac
     3421 gtgcgtatgt acgtttgcgg catgactgtt tacgattatt gccacttagg ccatgcccgt
     3481 gtgatggttg tgttcgacat gattgcccgt tggttgcgtg agtgcggcta tccgctcact
     3541 tatgtacgca acatcaccga catcgacgat aaaatcattg cccgtgcggc tgaaaacggc
     3601 gagaccatcg gcgagctgac tgcgcgtttc attcaggcta tgcacgaaga tgccgatgct
     3661 ttgggtgtgt tgcgtcccga tatcgagccg aaggcgacgg aaaacattcc gcaaatgatt
     3721 gccatgattg aaaccctgat tcaaaacggc aaggcatatc ctgccgcaaa tggcgacgtt
     3781 tactacgccg tgcgtgagtt tgccgcttac ggacaactgt cgggcaaatc gttggacgac
     3841 ctgcgcgcgg gcgaacgcgt ggaagtggac ggtttcaaac gcgatccgct tgattttgta
     3901 ttgtggaaag cggcgaaagc aggcgaaccg gcgtgggaaa gcccgtgggg caacggccgt
     3961 ccgggttggc acattgaatg ctctgccatg agtgaaaacc tgttcggcga taccttcgac
     4021 atccacggcg gcggcgcgga tttgcaattc ccgcatcatg aaaacgaaat cgcccaaagc
     4081 gtcggtgcca gcggtcatac ctgcggccac gaccacgcac aaacccacca cggtcaaagc
     4141 attgccagcc acgtcaaata ttggctgcac aacggcttta tccgcgtgga cggcgaaaaa
     4201 atgtccaaat cgttgggcaa cttcttcact atccgcgaag tgttgaaaca atacgacccc
     4261 gaagtcgtgc gcttcttcat cctgcgcgcc cactaccgca gcccgttgaa ctattccgac
     4321 gcgcatttgg acgacgcaaa aggcgcgttg acccgtctat acaccacctt gaaaaatacg
     4381 ccacccgccg atccgatgcc gtctgaagcc gacgatgact acacgcgccg cttttatgtt
     4441 gcgatgaatg atgattttga tacggtgaaa gcggtggcgg tgttgttcga acttgccggc
     4501 gaagtgaaca aaaccaatga cgcgcaactg gcaggctgcc tgaaagcctt gggcggcatc
     4561 atcggactgc tgcaacgcga cccgaccgag ttcctgcaag gcggcgcggc ttcagacggc
     4621 ctctccaacg aagaaatcga agacttgatt gcccggcgga aacaggcgcg ctccgataaa
     4681 aactgggcgg aatccgaccg cattcgcgac cttctgaacg aacacaaaat cattttggaa
     4741 gacaacgccg gcggcacgac ttggcggcgc ggttgaaatc catcgaaaag ggtaaatgtg
     4801 ccgcattttg cccttttttg ttcagacggc atttttattg tgagaactta tttttattat
     4861 gcttaaaatc atttccgcca acgtcaacgg catccgctcc gcctacaaaa aaggttttta
     4921 cgaatatatc gccgcatcgg gcgcggacat tgtctgcgtg caggaactca aagcgcagga
     4981 ggctgatttg tctgccgata tgaaaaatcc gcacgggatg cacggccatt ggcattgcgc
     5041 cgagaagcgc ggttacagcg gcgtggcggt gtacagcaaa cgcaaacccg acaatgtgca
     5101 aatcggtatg ggcattgaag aattcgacag ggaagggcgg tttgtgcgtt gcgatttcga
     5161 caggttgagc gttatttcgc tttacttgcc cagcggcagc agcgcggaag aacgccagca
     5221 ggtgaaatac cgtttccttg atgcgtttta ccctatgctc gaagcgatga aaaacgaagg
     5281 acgcgacatt gtcgtttgcg gcgactggaa catcgcccac caaaacatcg acctgaaaaa
     5341 ctggaaaggc aatcagaaaa attcgggctt cctaccggaa gaacgcgagt ggataggcaa
     5401 ggttatccac aagctcggtt ggacggatat gtggcgcacg ctttatcccg atgtgccggg
     5461 ctacacgtgg tggagcaacc gcgggcaggc gtatgcgaaa gatgtcgggt ggcgcatcga
     5521 ttatcagatg gttacgcccg aacttgccgc caaagccgtg tccgcacacg tttataaaga
     5581 tgaaaaattt tccgaccatg cgccgctggt catggagtat gactatgctg ccgaataaag
     5641 ttttgggtaa atatgattgg aatgtggacg gaaaaacagg aataggcgca gcatgggttg
     5701 tcgctgcgtt tatcctgccc atattggtgt gggcgatatt tatgttgtcg cggatgcaag
     5761 gctggcttgc gccgaccaag gcaaacccga catgggcgtt ggtgtggctg ctgatctgca
     5821 tgccctgcct gctgattgcg tccaaatgtt tgggttggaa aggctggcgg cgggtggtga
     5881 atatttttgc ctgcctgacc gtctgcgcca tattgagcgt acccgcttcg ctgctcatcg
     5941 cctttaccct ccgggacctg ctgaaatagg cggggcggta tgcagggttt tgaactcgaa
     6001 gatttgacgc tttggctgat acgcgatgcc ggagaggatc agatgtggat agaccgttgg
     6061 gcgatcagtt atcccgtcgt gcagatgtcc gaagcgtcgg ccggtcaaag cataggggaa
     6121 tggcaggcag ggcttcggac ggcatttgaa cgcatacgtg gcaaatacgt cgccgttgtc
     6181 gcacacggtg cgggcgcggc cgcatttttg gcgtggctgt atcaggcaga catcctgaca
     6241 cggaagaaaa ttgccaacat catccttgta ccgcagcgtc ccgatatttt tcccgacgat
     6301 gcggaacaca ctttccaacg cgtccgctgt ccctgccgtg ccgcattggt tgtacccgaa
     6361 cacggcggcg tgccgcacgg ttgggcgcaa aaacaggcgg atttgtggaa cgcccgcctg
     6421 ttggtttccc cgcattcggg cagtttgaac ggcatgctcg gcggctggca gtggggtatg
     6481 aagctgatgc aggaaatgtt gctggcgtga acgcttgcct tatcggaatt tcggttaact
     6541 ggggttgcaa ggaaaatgcc gtctgaaaca ggcttcagac ggcatttcca ttcaaacagg
     6601 gcttacaggc tgcccaacac gatacgcaat acgcggcgca gcggctcggc cgcgccccac
     6661 aagagctggt cgccgacggt gaacgcgctg atgtattcgc cgcccatacc cagtttgcgg
     6721 atgcgtccga cggggacgga cagcgtgccg gtaactttgg cgggggtcag ctcgcggatg
     6781 ctggcttctt tttcgttggg gatgactttc acccagtcgt tcgcgcctgc caaaatcgct
     6841 tcgatttcgg aaatgggcag gtcttttttc aacttcaggg taagggcttg gctgtggcag
     6901 cgcatagagc cgatgcggac gcacaggccg tcaatcacgg ttggattgtc gctgcggccg
     6961 aggattttgt tggtttccgc gccgcctttc cattcttctt tggactggcc gttgcccaaa
     7021 tccacgtcaa tccacggaat caggctgccg gcgagcggta cgccgaagtt ggctttcgga
     7081 tagtcttcgc tgcgcaggaa atcagatact ttgcggtcga tgtcgagaat cgagccggca
     7141 ggatcggcaa gttcgtccgc cacttgggcg tgaaccgcgc ccataccgct gatgagttcg
     7201 cgcatgtttt tcgcgcctgc gccggaagcg gcttggtagg tcatgctggt tgcccattcg
     7261 accaaatcgt tttggaacag gccgcccaaa gccatcagca tcagggaaac ggtacagttg
     7321 ccgccgatgt agtttttcac gccgtttttg aggccgttgt cgatgacgtt gcggttgacg
     7381 gggtcgagga cgataatcgc gtcgtctttc atacgcagcg aggatgccgc gtcaatccag
     7441 tagccgttcc agccgctgtc gcgcagggct tggaagacgg atttggtgta gtcgccgcct
     7501 tggcaggtaa cgatgatgtc catttttgcc agctcggcaa cgttgttcgc gtccaataat
     7561 gttttagccg cctgaccgaa atcaggggct gcgccgccga cgttggaagt ggtaaagaaa
     7621 aacgcttcgg gaatgtgggc gaagtcgttt tcttctttca tacgctgcat caaaaccgaa
     7681 ccgaccatac cgcgccagcc gacgaaacct actttcattg tgttgctcct tgaggaaaaa
     7741 tcgttaataa aattatgtat ggcgtaaagg aagcgtttta acgccgtatt gggaaaatgt
     7801 aaagttcttt atcgggattt ctttaagtat tggcgttttc ggtgtcgtgg cggtagaatc
     7861 ggggtcagtt aagaacaatt atcaggaacg gatatggaag aaaaaaacga ctacaccgac
     7921 gcggcatccg acaaccgcaa cgggcaggaa attgaagtcg gaattgccgg cgcaggcgac
     7981 cggatacttg ccgcgctgct gaaccaattg tttacctttt tggttttgtt ggtgccgttt
     8041 gccggactga ttgctttcgc catcaagaat gaaggcagga ttggcggcag tgaggaaata
     8101 ttcgggctgc ttttgggcat gacttccttt tgggtaggac tggcgggcat tttggtttat
     8161 accgtcgttc aaatctacta tatgagtcgg gacgggcagt cattgggtaa gaaaatcatg
     8221 agaatcaggg tgttgaaaac cgacggccgc aatcccggtt ttgtcggcac ggttttggta
     8281 cgcgaaatcg catggtcggt tttggttgcc attattgccg ccgttatcgg tcttgcagta
     8341 ggtgacaacg gagaaaacgc catcaacctg ctggcattcc ttgccaactt tgtcctgctc
     8401 tttatggtca aacgcgatcg ccgcacgctt tacgacatac tggcggatac ggttgtcgtc
     8461 aagctgccca aataaacgga cacggcaaaa tgccgtctga aagcctttca gacggcattt
     8521 ttatcttcag aaccgctcaa gcatcgtgca gtgaagccgc gtgcagggtg ttttccatca
     8581 atgtggcaat cgtcatcgga cccacgccgc cgggaacggg cgtaatcatc gccgcccgtt
     8641 cttttgccgt ttcaaattcc acgtcgccgc acaggctgcc gtcgtccaga cggttgatgc
     8701 ccacatcaat aacgaccgcg ccgggtttga tccattcgcc tttgacaaag ttcggaatgc
     8761 ctacgccgac caccaaaata tcggccccgg caacctcgtc tgccagattt tcggtcgcac
     8821 tgtggcagac cgttaccgtt gcgcgcgcca gcagcaattc caaagcctgc gggcggccga
     8881 caatattcga cgcgccgacc acgaccgctt ttttcccctt cggatcgatg ccgtaagcct
     8941 ccaaaagcgt catcacgccc ttgggcgtac acgggcgcat tagcggcatt ttgactgcaa
     9001 gcctgcccac attgtaagga tggaagccgt ccacgtcctt atccggcgaa atacgttcca
     9061 aaaccgcctg gctgtcgagg tgcttcggca gtggcagctg aaccagaata ccgtccactt
     9121 cggaatcgac attcaggcgg tcgaccagtg ccagcagttc ttcctgcgat gttgattcgg
     9181 gcagctcgta agacagtgat ttgatgccgc atttttggca ggaggttttc ttgttgcgga
     9241 cataaaccgc gctggcaggg tcgcctccga ccaaaaccac ggccaggcaa ggcgtgtgca
     9301 gattgttctg ttggcgttgc gccaccgctt cggcaaccgc ctgcaggcgt ttttgcgaaa
     9361 cttctttacc attgatcagt tgtgccgaca tatccgtcct tttgttaaac aggtagaaaa
     9421 tgctgaaaat tatcgcattt tccgtttgtt tttacagcag gatgccgaaa ataaatttgc
     9481 cctgaaataa aatacggctg tttggacgga ttaaatattt tttcaaatca aaaaactata
     9541 tttttcatct tggtgtcttg aaaataaatg ttgacgcatc tttccctgag ttgtatagtt
     9601 cgcttcttca agtcggggcg tagcgcagcc cggttagcgc atctgctttg ggagcagagg
     9661 gtcgtgagtt cgaatcccac cgccccgacc aatcaatcag cacgaatcgg catgagcgcc
     9721 cgtagctcaa ctggatagag caccgacctt ctaagtcggg ggttacaggt tcgattcctg
     9781 tcgggcgtgc caagaatttg attgaaaacc gtttttatgg tggctgtagc tcagttggta
     9841 gagccccgga ttgtgattcc ggttgtcgtg ggttcgagcc ccatcagcca ccccagataa
     9901 aggcccgtac ttttcggtac gggtcttttc ccgtttgcgc gtactgtccg cgccgccggc
     9961 tttgcctttc gcatatgctt ctgtatcatg tgggtttttc tgtcttttat ggtttttgtc
    10021 ggaggctttg atggttgacg cttggtctgt ccctgatttt gaatcgaaaa tctgtccgcc
    10081 cgaggcattg tccgaacggt tggctcggtt gccgcgcccg ctggtgttta ccaacggctg
    10141 tttcgacatc ctccacaggg ggcacgttac ttatctggcg caggcgcgtt cggcaggggc
    10201 ggcgttggtg ctggcgttga ataccgatgc ttcggtgcgg cgtttgggca aggacggcga
    10261 ccgcccgatt aatcctttgg agaaccgtgc cgccgttgcc gccgctttgg aaagtgtgga
    10321 tttggtaacg tggtttgacg gggatacgcc ggcggcgttg attgaggcgg tcaaacctga
    10381 gattttggtc aagggcggcg attgggctgc ggataagatt gtcggtgcgg cagaaacgtt
    10441 ggcgcgcggc ggtcaggtgt tttcaattcc gtttctgcac cagacttcga caacgaagac
    10501 tttggcaaaa atccgtgcgg cagagggcgg aaaatgacgg ttttgaagct ttcgcactgg
    10561 cgggtgttgg cggagcttgc cgacggtttg ccgcaacacg tctcgcaact ggcgcgtatg
    10621 gcggatatga agccgcagca gctcaacggt ttttggcagc agatgccggc gcacatacgc
    10681 gggctgttgc gccaacacga cggctattgg cggctggtgc gcccattggc ggttttcgat
    10741 gccgaaggtt tgcgcgagct gggggaaagg tcgggttttc agacggcatt gaagcacgag
    10801 tgcgcgtcca gcaacgacga gatactggaa ttggcgcgga ttgcgccgga caaggcgcac
    10861 aaaaccatat gtgtgaccca cctgcaaagt aagggcaggg ggcggcaggg gcggaagtgg
    10921 tcgcaccgtt tgggcgagtg tctgatgttc agttttggct gggtgtttga ccggccgcag
    10981 tatgagttgg gttcgctgtc gcctgttgcg gcagtggcgt gtcggcgcgc cttgtcgcgt
    11041 ttaggtttgg atgtgcagat taagtggccc aatgatttgg ttgtcggacg cgacaaattg
    11101 ggcggcattc tgattgaaac ggtcaggacg ggcggcaaaa cggttgccgt ggtcggtatc
    11161 ggcatcaatt ttgtcctgcc caaggaagta gaaaatgccg cttccgtgca atcgctgttt
    11221 cagacggcat cgcggcgggg caatgccgat gccgccgtgt tgctggaaac gctgttggcg
    11281 gaacttgatg cggtgttgtt gcaatatgcg cgggacggat ttgcgccttt tgtggcggaa
    11341 tatcaggctg ccaaccgcga ccacggcaag gcggtattgc tgttgcgcga cggcgaaacc
    11401 gtgttcgaag gcacggttaa aggcgtggac ggacaaggcg ttctgcactt ggaaacggca
    11461 gagggcaaac agacggtcgt cagcggcgaa atcagcctgc ggtccgacga caggccggtt
    11521 tccgtgccga agcggcggga ttcggaacgt tttctgctgt tggacggcgg caacagccgg
    11581 ctcaagtggg cgtgggtgga aaacggcacg ttcgcaaccg tcggtagcgc gccgtaccgc
    11641 gatttgtcgc ctttgggcgc ggagtgggcg gaaaaggtgg atggaaatgt ccgcatcgtc
    11701 ggttgcgccg tgtgcggaga attcaaaaag gcacaagtgc aggaacagct cgcccgaaaa
    11761 atcgagtggc tgccgtcttc cgcacaggct ttgggcatac gcaaccacta ccgccacccc
    11821 gaagaacacg gttccgaccg ctggttcaac gccttgggca gccgccgctt cagccgcaac
    11881 gcctgcgtcg tcgtcagttg cggcacggcg gtaacggttg acgcgctcac cgatgacgga
    11941 cattatctcg ggggaaccat catgcccggt ttccacctga tgaaagaatc gctcgccgtc
    12001 cgaaccgcca acctcaaccg gcacgccggt aagcgttatc ctttcccgac cacaacgggc
    12061 aatgccgtcg ccagcggcat gatggatgcg gtttgcggct cggttatgat gatgcacggg
    12121 cgtttgaaag aaaaaaccgg ggcgggcaag cctgtcgatg tcatcattac cggcggcggc
    12181 gcggcaaaag ttgccgaagc cctgccgcct gcatttttgg cggaaaatac cgtgcgcgtg
    12241 gcggacaacc tcgtcattca cgggctgctg aacctgattg ccgccgaagg cggggaatcg
    12301 gaacatactt aaaaaataca ccctttttca acacggggcg gtatccgtcc gaatccgtcc
    12361 ggattctgcc gcccgacaga ctgccgccca ggcgggcggg gtttcaaaca ttgaggaaat
    12421 tctaatgaaa tggctcttca cccttttggc ggtactcaat attgccgtat tcggcggcac
    12481 ggtgggctat aaattggccg ttaaagcgac gggcggcgtg ccggaaaacc gggccgtcga
    12541 aaacacgccc cccgcaacgc ccgccgccga caatgcggcg gcatccgtgg aggatacggc
    12601 ggcactgctc aaaccgggcg acatcctgac cgaagagcag gccgagcagt tgcgcttgaa
    12661 aaaggaagcg gaacagaaaa aactgaggga gaaaaaacag cgcgaggaaa aagcccgccg
    12721 cgaaaaactc gccgccgaaa aggcgcaggc ggaacgcgaa aacggcgcgg cggatgcctt
    12781 atgcgccgcg caggcaagcc tcacgatgga cgaagacgac taccaccgca tcaaagggct
    12841 tttgggcaaa tggtcgcacg ttgccagcag gagcgtcgaa aaacgcaccg ccaaagccaa
    12901 acctgccgac aaaacctacc gcgtcgtcct gcccgtttcc gccgatgccg aaaatcaggc
    12961 ggcggagctg tctgccaaag gtttcaaccc cataccgttt gacggcgcat tgagtttggg
    13021 tgtcggcaac agccgggaaa acgcccaagc cctgcaaaac cggcttgccg gcgccggatt
    13081 cggcggggcg catattgtcg aacactttgc cgaagccgac aggcaggacg attctttgag
    13141 cgtgtcgcgt atgacggttt tgtttaccgg cgtgaatgct gccgatgcgg acgaaattcg
    13201 taaaatcacg tccctatacg gcaaactgaa cctcaagtct tgcaaatagg cgcgaaagcc
    13261 ggacgataaa cgccaaaccc accgcgaagg tgggtttggc gtttcggttt tgagtatctg
    13321 atatataaaa agggaatgta tgtctcgtat atgccgactc caagtgtgaa agtgaattgt
    13381 gagggaatac agtgtttaat ggttcggaat ggacgacttc cgagtggttt tgattatacg
    13441 ctaataataa taattatcaa taaaaattta aaaataaatg cggatttttt gtaatacgat
    13501 gaaaaataaa caaattaacc tgtccgccga tgtttgtgat tcgggtcggc aaactttcat
    13561 tgtgtcggac aaatcggggt cggcagtcat agctgagggc ttcgtgcctg acggaaataa
    13621 gcctgccgtg tttgaacatc cgtccgattt ggcgcggaca gggttgtcat tcgggggtgt
    13681 ttgagcgtgg attcggggat gcaaaagcgg taaaaccgtt gcggatgaag gcttacggac
    13741 ggctttaggt atttttaagt gtattcaaac aacaacaaaa cgcttcttct attgccgtat
    13801 ttagggcttg cgcggcagcc tgcacaccgt ggaggccgag tgtttcgttg gttgcggcat
    13861 cgggacgggc ggcaaggttt tccggccggt ttttccattt tttccgacag agccgtgatg
    13921 cttgggaatg gcggtttgcc cgtttcagac cgccgccgtt ttgggctgcg atgccgtctg
    13981 aaccgttcag acggcatttt tgccgccttt ttcaatattc cgccgaatgc caaaacggtt
    14041 gtccgactgt cggcgtgctg gcttgcgctt tgtcgcgtgc ttcgaccggt ccggcttcaa
    14101 atgccagccg tccggattcg acggcgagtg cgaaggcgcg tgccatattg accggatcgc
    14161 cgctgcggga aacggcagta ttcaaaagca cgccgtcaaa gccccattcc atcacttgtg
    14221 ccgcctgtga gggcaaaccc aagcccgcgt cgataatcag cggcgtgtcg ggcaggcgtt
    14281 cgcgcaggac gttcaacgcg taggcgtgaa ccgcgcccaa acccgtgccg atcggggccg
    14341 cccacggcat caacgcctga cagcccgcgt cgagcaggcg gcggcaggca atcaggtctt
    14401 cggtgcaata aggcagcact ttgaagccgt ctttaatcag gatttccgcc gcttcgacaa
    14461 gctggaacac atccggctgc aaggtgtcgt catctccgat gagttccaat tttatccaat
    14521 cggtttcaaa cacttcgcgc gccatttgcg ccgtcgttac cgcttcctgc acgctttggc
    14581 agcctgccgt gttcggcagg acgggaacgc cggtttcttg aagcagcgac caaaacccct
    14641 gaccgtgcgc ctcgccgccg cttcccgcgc ggcgcagcga gacggtaatc atcgcaggct
    14701 gggcggtttg gatggattgt ttgaggattt cgggggtcgg gtaggcagcc gtgccgagca
    14761 gcagccgtga agggaaagtt tctccgtata gggtgagcat aatgggttcc tttgtatagt
    14821 ggattaacaa aaaccagtac ggcgttgcct cgccttagct caaagaaaac gattctctaa
    14881 ggtgctgaag cactaagtga atcggttccg tactatccgt actgtctgcg gcttcgtcgc
    14941 cttgtcctga tttaaagtta atccaccata acgttgtttt ggggacaggg gtcgtctgaa
    15001 aaggcaaaac cgcctagccg ccgacgacgg ggcggacgat atcgattttg tcgttttcgt
    15061 tcaaaaccgt ttccgcatac gcgcctttgg ggatgaaaac ggtgttgacc gccacggcaa
    15121 agggctgttg cggcgcggtt tgggcgatga ggtcggcaac gcttgtgccg tgaagttcgg
    15181 cgggtccgcc gtttaagatg atgttcatgg tttctcgatg tttct
//
LOCUS       DAWKST010000037        14902 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.037, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000037 DAWKST010000000
VERSION     DAWKST010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14902
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.037"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(87..3572)
                     /gene="smc"
                     /locus_tag="WJL87_000786"
     CDS             complement(87..3572)
                     /gene="smc"
                     /locus_tag="WJL87_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ7799242.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNRQQDLLDYAQWQQSLAAADKATEQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQLQRIHQE
                     QQQIRVQLEENELYVEEKQTELAEWAMQVAEHEERLPELEETQTALNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARAVPQGFVPPEPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHYWLDGVLCAPDLSYALAHQNDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELENLAPELSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIVTLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIAQLEQQTLDWQERQQELVLAYETEFQNDEQHIKLDELT
                     ESVHTLDEEYIAVQEKLTQIQEQGREQYARVQTLQTQLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITILEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            3574..3825
                     /locus_tag="WJL87_000787"
     CDS             3574..3825
                     /locus_tag="WJL87_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799243.1"
                     /translation="MVGKNKKEAYFNGNPAAPPHILPAKPYRIPHPRCRLKPSDGIFN
                     APEPRFRQRPMLGAAFAVLPRRRARYNPPRHPPDLETCL"
     gene            3822..4544
                     /gene="lpxH"
                     /locus_tag="WJL87_000788"
     CDS             3822..4544
                     /gene="lpxH"
                     /locus_tag="WJL87_000788"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ7799244.1"
                     /translation="MKPAYFISDLHLSEKHPELTALLLRFLRSSAAGQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYQRFRKIVHRKRLQKLFLMLPLKWRTRLATKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFGAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKY"
     gene            4813..6399
                     /locus_tag="WJL87_000789"
     CDS             4813..6399
                     /locus_tag="WJL87_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ7799245.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     GDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYSVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(6567..6770)
                     /locus_tag="WJL87_000790"
     CDS             complement(6567..6770)
                     /locus_tag="WJL87_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ7799246.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            6886..7383
                     /locus_tag="WJL87_000791"
     CDS             6886..7383
                     /locus_tag="WJL87_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HEZ7799247.1"
                     /translation="MTALTLPEDIRQQEPSALLYPLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            7465..8658
                     /locus_tag="WJL87_000792"
     CDS             7465..8658
                     /locus_tag="WJL87_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ7799248.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEATAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     ASQDFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(9061..9996)
                     /gene="hemC"
                     /locus_tag="WJL87_000793"
     CDS             complement(9061..9996)
                     /gene="hemC"
                     /locus_tag="WJL87_000793"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ7799249.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYARLEEMPKGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLKLDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLQADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            10115..10630
                     /gene="ybeY"
                     /locus_tag="WJL87_000794"
     CDS             10115..10630
                     /gene="ybeY"
                     /locus_tag="WJL87_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ7799250.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGVLHLMGYDHIEDDEAEIMEAEEIRLMLAA
                     GFPNPYQEDEY"
     gene            10632..11456
                     /locus_tag="WJL87_000795"
     CDS             10632..11456
                     /locus_tag="WJL87_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEZ7799251.1"
                     /translation="MDGTQSKPKFFERLISRLAGEPDSAEDVLTLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(11585..12964)
                     /gene="nhaC"
                     /locus_tag="WJL87_000796"
     CDS             complement(11585..12964)
                     /gene="nhaC"
                     /locus_tag="WJL87_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ7799252.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPNVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVILALMRINAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVVKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAIRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDRLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(13255..14478)
                     /locus_tag="WJL87_000797"
     CDS             complement(13255..14478)
                     /locus_tag="WJL87_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEZ7799253.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTGVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAIAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         3521 a         4201 c         3826 g         3354 t
ORIGIN      
        1 tttaaattta attcactata ccgtctgaaa acctgtatcg caacacgtca ttaaaaacgg
       61 caagattcag ccgttctgca acccgttcaa acggcttccg ccatttccaa cgcctgcttg
      121 atgtccacgg cgacgacgcg cgatacgcct ttttcctgca tggttacgcc gaccagctgc
      181 tctgccattt ccatggtcag gcggttgtgg gagatgtaga ggaactgggt ttgcgccgac
      241 atttctttga ccaggttgca gaaacgcgag gtgttggcgt cgtccagcgg ggcatcgacc
      301 tcgtccaaaa ggcagaacgg agcggggttg aggctgaaca gagcgaacac gaggctcatg
      361 gcggtgaggg ctttttcgcc gccggagagg aggtggatgg tgctgttttt cttgccgggc
      421 ggacgcgcca taatggacac accggcggtc agtaggtcgt cgcctatcat tttgagagtg
      481 gcttcgccgc cgccgaacag ggtcgggaag aaggtttgga ctttgccgtt gacggcgtcg
      541 aaggtttctt tgaaacgcgc tttggttttg tcgtcgattt gggcgatggc ttcttccaaa
      601 atggtgatgg cggcctgcac gtcttcgctt tggctgcggt agtagccgtc gcgttcgcgc
      661 gcttcttcga gttcttgcag ggcggcgagg ttgaccgcgc cgagggcttc gatttgttgc
      721 gagaggctgc cgatgctgct gttcaatact ttcggcgatt ctttcgccaa cgcttcgagc
      781 gcatccaaat cggcggcgcg ttcggtcagg ttttgatggt agcgtttggc gttgatcagg
      841 gcttcctgtt gttgcaacag ggcggtttgg gtggcggcct gaagctgtgg caactgggtt
      901 tgcagggttt gtacgcgggc gtattgttcc ctaccctgct cctgaatctg cgtgagtttt
      961 tcctgcacgg cgatgtattc ttcgtccagc gtgtgtacgg attcggtcag ctcgtcgagc
     1021 ttgatgtgct gctcgtcgtt ttggaactcg gtttcatagg cgaggacaag ctcttgctgg
     1081 cgttcctgcc agtcgagggt ttgctgttca agctgggcga tttgctgccg gtagttttgt
     1141 ttttgctggt tgagtttgtg gacggcgact tcggcaagcc catattggcg gttggcttcc
     1201 aacagggcaa gctgcgcctg tttcagacgg ccttgctgct cttggcggct gtgcgcggtg
     1261 gtttgctgct ggtgttcgag ttcggcggcg gcttcctgca aggtaacgat gtcgtctgaa
     1321 agcccgtcgg acgtgtgttg caacacggtc tgttcttccg ccaactgcgc cagttcgcgc
     1381 tcgatgtgtt cgcggcggat ttgcccttgg ttggtacgcg ctaagagttc ggcggcgcgt
     1441 tgctgcgcct gactgtattg gcgcgtgtgc tgctgttgct gctgcatcag gtttttatgt
     1501 tgcacttcag acgagcgcac ggcagcttcc gcctgtttga acgcggcttc ggcggcggaa
     1561 agttcggggg cgaggttttc cagttcggac gcgatgccgt cgaggcgcgc tttttgggca
     1621 atcaggcttt cctgcgcggg tttggcatag agcaggacgc tgactttatc gacctgatga
     1681 ccttcgggcg tgagccagat ttggtgtgcg cccaaatcgt tttgatgcgc gagggcatag
     1741 ctcaaatcgg gcgcgcacaa tacgccgtcg agccagtagt gcaatgccgt ctgaaacggc
     1801 ggctgcgctt ggatttggtt cagcaatgcc tgtacgggca gggatttttt gatgccgccg
     1861 gagagaccgt ctgaaagcca cgccgcctgc ccctgcggca aaggttcggg cggtacgaaa
     1921 ccttgcggca cggcgcgggc gtgcaggcgt tctgcaagga tgacggacaa ggcgtgctgc
     1981 cactcggcag gcgcggtgat gtgttgccac agctgcggcg cggcggcgtg gtcggttgcc
     2041 tgccagaaat cggctgcttc ctgctgttgc gacaggattt gcgacaacgc ctgctgctgc
     2101 gcctgcaagg tgatatgttg ctgcttcagg ctttggaagc ggtttgaggc cgtctgaaac
     2161 gcctcgcggg cggcgtgtaa ggcttcttcg gcggcgatga tttgttcttc gtaatgctct
     2221 tgccggcttt gcaacaaggc agcggcctcc tgcgcggcgg cggtttccgc ttcgtcgggc
     2281 aggttcaggg cttggttctc ctgtttcaga cggcctttgc gctcttcgtg cttggcaacg
     2341 gtttgttcgg catgggcaag ctgctgctgc ttcaacgcca gttcgcggcg gatgcggttt
     2401 gcctcgtcct gctgggtttg gaaggcggca ttgagcgcgg tttgggtttc ttccaattcg
     2461 ggcagacgct cttcgtgttc ggcaacctgc atcgcccact ccgccagctc ggtttgcttt
     2521 tcttcgacgt ataactcgtt ttcttcaagc tgcacgcgga tttgctgctg ctcttgatga
     2581 atacgctgca actgcgcctg cgccgcctgc ttgtcgcgtt cgatgcgttg gtgcaggttt
     2641 tgctgatggc ggatttgttc ttccaaacgg gcaatctgct cgcgcaacac gccgcgcttg
     2701 ttgctcaatt catgcaccgc ctgttgctgc gactgttcgg cggtctgcaa ggcgtgtact
     2761 tcgtcgttta acgcctgaac ctgcgcggcg gtttcgtcct gctgcgcctg caaagactga
     2821 tgctgctcgg tcgccttgtc ggcggcggca agcgattgct gccattgggc gtaatcgagc
     2881 aaatcctgtt gccggttcaa ctgcgcggtc agggatttgt agcgttcggc ggtttccgcc
     2941 tgtttctcca gcttttccac ctgacgcgcc aattcgttct gcaaatcgcc caaacgctgc
     3001 aaatgctcgc gcgtgtcttt cagacggcct tccgtctcct tgcggcgttc cttatatttg
     3061 gacacacctg ccgcctcctc gatataggcg cgcaattcct ccggccgcgc ttcgatgatg
     3121 cgcgaaatca tcccctgctc gataacggca taaccgcgcg cgcccacgcc cgtacccaaa
     3181 aataaatcgg taatgtcgcg gcggcgcacg gtctgattat tgatgaaata agtcgattcg
     3241 ccctgacgcg tcagctgccg cttgatactc acctcggcat actgccccca cgccccctgc
     3301 aaactgtggt cgctgttgtc aaacaccagc tccaccgaag ccctcggcgc aggacggcgc
     3361 gtcgccgcac cgttaaaaat cacgtcctgc atactctcgc cgcgaagctg cttcgccgaa
     3421 gcctcaccca acacccagcg caccgcgtca atcacattcg acttgccgca accgttcggc
     3481 ccgataaccg cgacaagctg ccccggaaca tgaatcgtgg tcgggtcggt aaaagatttg
     3541 aagccggaga gtttgatgtg ggtcaggcgc ataatggtcg gaaaaaataa aaaagaagcg
     3601 tattttaacg gaaatcccgc cgcaccaccc catatcttgc cggcaaaacc ttaccgcatc
     3661 ccgcaccctc gatgccgtct gaagccttca gacggcattt ttaacgcgcc cgaaccccga
     3721 ttccgccaac gcccgatgct tggcgcggca tttgccgtat tgccccgccg ccgggcgcgt
     3781 tataatccgc cacgacatcc gcccgatttg gaaacctgcc tatgaaaccc gcctatttca
     3841 tctccgacct gcatttgagc gaaaagcacc ccgaactgac cgcgctgttg ctgcgttttt
     3901 tacgttcttc cgccgccggg caggcgcggg cggtttacat tttgggcgat ttgtttgatt
     3961 tttgggtggg cgatgacgaa gtttccgagt tgaatacttc ggttgcgcgt gaaatcagga
     4021 aattgtccga caaaggcgtt gccgtgttct tcgtcagggg caaccgcgac ttcctgatcg
     4081 gtcaggattt ttgccggcag gcgggcatga cgctgctgcc ggattactcg gttttggact
     4141 tgttcggctg caaaaccctc atctgccacg gcgacactct gtgtacagat gacagggcat
     4201 accaacgttt ccgcaaaatc gtgcatcgga agcggctgca aaaactgttc ctgatgctgc
     4261 ccctgaagtg gcgcacgcgc cttgccacca aaatcaggcg tgtcagcaaa atggaaaaac
     4321 aggtcaagcc cgccgatatt atggatgtca atgccgcctt taccgcgcgg caggtacgcg
     4381 ccttcggtgc ggaaaggctg atacacggac acacccaccg cgagcatatc catcacgaaa
     4441 acggctttac ccgcatcgtt ttgggcgact ggcataacga ctatgcttca atcctccgcg
     4501 tggacgggga cggcgcggta ttcgtgccgc tggaaaaata ctgaaaatgc cgtctgaagc
     4561 ctttcgggcg gcttttttta cacgcccctt tcccacactc cctccgccgc atcatcacca
     4621 tccttaaccc gcattaatgg gcgttatcga taaaatgctt gaccgattac ataagattac
     4681 gtaaagtgtg caaatgcata acaattggtc ttaccaattt atcttttaac caaaatatcg
     4741 ggtgtacggg atttgaacgt ctttccaaac ctcgacgcgc cttatgatgc aaatgcctaa
     4801 aaaggattcg ttatggcact tttcctcagc atattcccca tcgtcctgct gatttggctg
     4861 atggtgaaaa aaaacagtat gccctcctac gtcgcgctgc cgattaccgc agtgctgatt
     4921 tacgccatca aacttttcta cttcggcgat gcgggcatgc tgctcaacgc caccgccgct
     4981 tccggcctcg tcaaaacgct cacgccgatt accgtgattt tcggcgcgat tatgttcaac
     5041 cgtatgatgg aaaccacggg ctgcatcgat gtcatccgca aatggctggc gaccatcagc
     5101 cccaaccccg tagcgcaact gatgattatc ggctgggctt ttgcctttat gattgaaggc
     5161 gcatccggct tcggcacgcc tgccgcgatt gccgcgccga ttctgatgag cttgggcttc
     5221 aacccgttga aagtggcgat tttcactttg gtgatgaact ccgtccccgt ctctttcggc
     5281 gcggtcggta cgccgacctg gttcggtttc gcaccgctga acctgagtgc cgaagacatc
     5341 ctcgccatcg gcaggcagac cggcgtaatg cacttcttcg caggtttcgt catccccgtc
     5401 atcggcttgg gcttcatcgt accttggtct gaaatccgca aaaacttggg cttcgtcgcc
     5461 attgccgtct tctcctgcac cattccttat gtcgcattgg cgatggtcaa cgaagaattc
     5521 ccgtcgctcg tcgccggcgc aatcggcctg atggtgtccg tattcgccgc caaccaaggc
     5581 tggggcttga gcaaagacca cgccaaagac ccgaatgccg aaaaagtgcc gttcgcccaa
     5641 gtcgccaaag cactcgcccc tttgggtatg ctgatcggca tgctggtggt tacgcgcatc
     5701 aaacagctcg gcatcaaagg cattttgacc agcaaagaag aatggttcag cttccaactg
     5761 ccgtttgatt tgtccaaaat caccgtcagc gactccctga cgattacctt cggcaatatt
     5821 ttcggacaag atgtcagcgc gtcttaccaa acgctgtacg tcccggcttg gattccgttt
     5881 gtgctgaccg tttggatttg catcctgctg tataaaacca aattcaaaga tgcctggacg
     5941 atttattccg taactttcaa tcaaaccaaa aaaccgctgc ttgccctgat gggtgcgctg
     6001 attatggttc agctgatgct ggtcggcggc gacaattcga tggtgaaaat catcggtaag
     6061 gaatttgccg caatggcggg cgaacactgg gtttatttct cgccgtatct gggcgcgatc
     6121 ggtgcgttct tctccggctc caacaccgtg tccaacctga ctttcggccc gattcagcag
     6181 caaatcgcct tggataccgg cctgtccgtt accctgattc tggcgttgca gtccgtcggc
     6241 ggcgcgatgg gcaatatggt gtgcctcaac aacatcatcg ccgtatgtac cgtattggat
     6301 gtgaaaaatt ccgaaggtgc gattatcaag aaaaccgtta tcccgatggc gatttacggc
     6361 gtgattgccg tcgtcgcggc aatgattttc ttcctctagg cgaaacgcca ccatccatag
     6421 cggcagcacg cggggaacgg aaaccgccgt tccccgtctg ggcggcgcgt cgggcagaat
     6481 cccggtgccg gaacagcccg ccccgccaaa caaatgccgt ctgaaaccgg aaaaggcttc
     6541 agacggcatt tttccgccgt ttgcattcag gcgtaatgat gatgcggcat cttgccgtca
     6601 aggcggtagc gcgtgccgca atacgggcag gcaacgctgc ccgattctcc ttcgcacaaa
     6661 ggcaaaaaca ctctcggatg cccgttccac tgctcgttgt cgggtcccga gcaatacagc
     6721 ggcagatttt ccggcaacac ggaaatttcc tgcagattca gattgtccat ttgattttcc
     6781 tttgcgtggt tggcgtgcgc ccattttacg ccatcggcag gctaaaggat attttcggcg
     6841 caaagccgca atccgctata atccccactt ttcagacggc ataccatgac tgcgcttacc
     6901 cttccggaag acatccgcca acaagagcca tccgccctgc tctatcccct cgtttccgcc
     6961 tacctcgaac acaccgccca aaccggcgac gaatccctct cctgcctgtc cgacgaccag
     7021 cacacgctga ccgcattctg ctacctcgac agccaagtcg aagaaggcgg cttcgtgcaa
     7081 ctcatcgcct ccggttacgg cgaatatatt ttccgcaacc cgcttgccga cagcctgcgc
     7141 cgctggaaaa tcaaagccgt gccgaaagtc ttggacaaag ccaaagccct ctacgaacaa
     7201 cacggcaaaa ccatcgaaac gctcgccgac ggaggcgcag acatcgaatc cctgcgcaaa
     7261 cagttccccg actttgaaga atgggacggc gcatactacg aagccgccga acaagacctg
     7321 cccctgcttg cagaacacat acagtcaaac tgggaaacct tcgcccatat cgggcaggcg
     7381 tgattgcgtc tgtttccagc ccgtgtaaaa cagcgtaaaa tcggcaaacc cgaatgacct
     7441 tccgtcccca tcaaggagca agctatgttc ttcaagcaca tcgaagccgc ccccgccgat
     7501 ccgattctcg gtttgggcga agcattcaaa gccgaaaccc gccccgaaaa agtcaacctc
     7561 ggcatcggcg tttataaaga cgcatccggc gcgacacccc tcgtcaaagc cgtcaaagaa
     7621 gccgaaaaac gcctgttgga aagcgaaacc accaaaaact acctcaccat cgacggcgtt
     7681 gccgactaca acgcgcaaac ccaaatcctg ctgttcggca aagaccacga aatcatcgcc
     7741 agccgtcgcg ccaaaacagc gcaaagcctc ggcggtacgg gcgcattgcg tattgcggcc
     7801 gagtttgcca aacgccagtt gaacgcgcaa accatctgga tttccaatcc gacttggccc
     7861 aaccacaacg ccatcgccaa agcggtcggt atccaagaca aaccttatcg ttactatgat
     7921 gccgccaaac acggtttgga ttgggacggc atgattgagg acttgagcca agcgcaaaaa
     7981 ggcgacatcg tcctgctgca cggctgctgc cacaatccta ccggcatcga ccctacgccc
     8041 gaacaatggg aaactttggc aaaactttct gccgaaaaag gctggttgcc gctgtttgac
     8101 tttgcctacc aaggcttcgg caacggtctg gaagaagatg cctacggctt gcgcgtgttc
     8161 ttgaaacaca atacagaatt gctgattgcc agctcttatt ccaaaaactt cggtatgtac
     8221 aacgagcgcg tgggcgcgtt cactttggtg gccgaagatg aagcaaccgc cgcccgcgcc
     8281 cacagccaag tcaaaaccat catccgtacc ttgtattcca atccggcttc acacggcgcg
     8341 aacactattg cgctggtgtt gaaaaatgat gatttgaaag cacaatggat tgccgaactc
     8401 gatgaaatgc gcggccgcat caaagccatg cgccaaaaat ttgtcgagtt gctcaaagcc
     8461 aaaggtgcaa gccaagactt tgatttcatt atcgaacaaa acggtatgtt ctctttcagc
     8521 ggtctgactc ccgaacaagt tgaccgtttg aaaaacgagt ttgccattta tgccgtccgc
     8581 tccggccgca tcaacgttgc cggcattacc gacgacaaca tcgattatct gtgcgaaagc
     8641 atcgtgaaag tactgtaaaa agcacagcca tatgaaaatg ccgtctgaac cttaaccagt
     8701 tcagacggca tctgtttttt gttaaaaaac aataaactat aacaatttca aacaagctaa
     8761 aaacatctat aaaatcaacc caatatgatg aactcaaata aaaatacgaa tatcatcatt
     8821 ctcaaattca aaatttgaac gccacagtat tatccgaagc cacaaaaacc tttccgccgt
     8881 cattcccgcg caggcgggaa tccgggaaac caatgctgca agaattgatt agaaacaact
     8941 gaaaccgagc aaactggatt cccacctgcg cgggaatgac gaaataaggc gagtaaaact
     9001 gatgaaacaa attgattaaa aacaataact tatattattt tatttaaatt ttcgataaaa
     9061 tcaattttcc gtattcaata ctgctccaat caattcccgc gcaccgtcgt ccgccaattt
     9121 cttagcgaca gcgcgtccga gcgcgtcggc atattcggca ggggcttgcg cgtccgcctg
     9181 caacacaacc gaaccgtcgg ggtgtccgac cagtccgcgc aaggtcagca agccgttttc
     9241 ttccgtgcaa tatgcggcca gcggcacttg gcagcttccg cccaaagcgc gcgccaaagc
     9301 tcgttcggcc gtaacgcagg cgtgtgtggt atcgtggttt agcggtttta aaacctcata
     9361 caaatcttcg cggtgcgcgg caatttcgat acccaatgcg ccttgtccgg cggcaggcag
     9421 gctgtcggat tccgacaaaa tcatgcggat gcgtccgtcc aatttcagac gctgcaaacc
     9481 ggcggcggcc aaaataattg cgtcgtattc gccgttatcg agtttggaca aacgggtttg
     9541 cacattgccg cgcaaaggtt tgataagcaa atgcggatag cgcgcgcgca actgggcttc
     9601 gcggcgcagg ctggatgtac cgacgacagc gcctttgggc atttcttcca aacgcgcgta
     9661 ttggttggac acaaacgcgt caaacggatt ggcgcgttcg ccgatggcgg caagcgcgaa
     9721 accttcaggc aaatccatcg gcacgtcctt aatcgaatgc accgccaaat cggcgcgccc
     9781 gtcatataaa gcctgttcca actctttgac aaacaagcct ttaccgccga cttttgacaa
     9841 agttttgtcc aaaatctgat cgccgcgcgt ggtcatgccc aaaatctcga cttcgcaatc
     9901 gggatacaga gccttcagac ggccttggat atgctttgcc tgccacatag caagcaggct
     9961 ttcgcggctg gcgatgacga gttttttcgg gttcatgttc aatgcgttca aagaatggaa
    10021 acggcgtgaa gtctatcata ttttgaacgt ttcttcagac ggcaccgtca ggtctatgtt
    10081 atatttacac ccacaaaaca cgaaacccgc cacaatgaaa cgcaccaaaa aatacccttt
    10141 cttaacgttg cagcggcaac gtttccattt gaactttgaa aacgcctctt ccgccgccgg
    10201 catcccagcc gaacgcgatt tctaccgctg ggcgtggtct gccttgaaaa atgaataccg
    10261 ccgcgccgac atcagcctga ttcttctgga cgaagaagaa gcccgagcct acaaccgcga
    10321 ctaccgcggc aaagattacg ccaccaatgt attgagtttc gcgctcaacg aaggcgaaat
    10381 cctgccctgc caagtttcgg aaaaactgta cggcgatttg attatctgcc cgcaagtggt
    10441 tttgaaagaa gccgccgaac agggcaaaac gcccgaacgg cattttgccc acctgaccat
    10501 acacggcgtt ttgcacctga tgggctacga ccacatcgaa gacgatgaag ccgaaataat
    10561 ggaagccgaa gaaatccgcc tgatgctggc ggcaggcttc cccaacccct accaagagga
    10621 cgaatattaa aatggacggt acgcagtcga aacccaagtt tttcgaacgc ctgatctccc
    10681 gacttgccgg cgagcccgac tccgccgaag acgtattgac cctgctgcgc caagcgcacg
    10741 agcaggaagt ttttgatgcg gatacgcttt taaggttgga aaaagtcctc gatttttccg
    10801 atttggaagt gcgcgacgcg atgattacgc gcagccgtat gaacgtttta aaagaaaacg
    10861 acagcatcga acgcatcacc gcctacgtta tcgataccgc ccattcgcgc ttccccgtca
    10921 tcggtgaaga caaagacgaa gttttgggta ttttgcacgc caaagacctg ctcaaatata
    10981 tgttcaaccc cgagcagttc cacctcaaat cgatattgcg ccctgccgtc ttcgtccccg
    11041 aaggcaaatc gctgaccgcc cttttaaaag agttccgcga acagcgcaac catatggcga
    11101 tcgtcatcga cgaatacggc ggcacgtcgg gtttggtcac ctttgaagac atcatcgagc
    11161 aaatcgtcgg cgaaatcgaa gacgagtttg acgaagacga tagcgccgac aatatccacg
    11221 ccgtttcttc cgaacgctgg cgcatccatg cagctaccga aatcgaagac atcaacacct
    11281 tcttcggcac ggaatacagc agcgaagaag ccgacaccat tggcggcctg gtcattcaag
    11341 agttgggaca tctgcccgtg cgcggcgaaa aagtccttat cggcggtttg cagttcaccg
    11401 tcgcacgcgc cgacaaccgc cgcctgcata cgctgatggc gacccgcgtg aagtaagcac
    11461 cgccgtttct gcacagttta ggatgacggt acgggcgttt tctgtttcaa tccgccccat
    11521 ccgccaaaca taaaaatgcc gtctgaaacc gttgcaagtt tcagacggca tttcgcttat
    11581 ccgcttattt tttgctcaaa gtcagccccg tccaaccgaa taacagggtc aaagccaaac
    11641 tcaaatagca gaaaaaggca tacggcagat attcccaaac cggcacgccc agcgcgtggc
    11701 tgatgaacac gccgcatacg ctccacggta cgagcgggtt aatcaccgtc cccgcatctt
    11761 ccagcgtccg cgacagattg cgggaatgta gtccgagcct gtcgtaaacg ggtttaaacg
    11821 tttctccaga aagcaggatg ctcaaatatt gttcgccaat caggaaatta accccgaccg
    11881 aagtcatggc aacgctgaac gtcgcgcgtc cggcattcgt caagaaggta cggatggcct
    11941 ccaacaggga aggaatcaca ccgagcgcaa acaacagtcc gcccaaactc atcccgagaa
    12001 tcacgatggt ttgcgtgaaa aacatacttt ccaaaccgcc gcgcgaaatc agtttgacaa
    12061 catctttaaa cgcttcgcct tcgagtttgt agccgccgta aaaccacgca ccgagctgac
    12121 gcagatcggg cgtgctgtgc agatacgtta cagcaacggc aaccatgacg gtaaagagca
    12181 tggcgacgac ggcgttgatg cgcatcaatg ccaaaatgac caacagcgca aacggaatca
    12241 gcgaatagcc gtgcaccaat cccgtggctt caagctggct gcggaaggat tcgacgctgt
    12301 tcaaatcctg cgcggcgaca ttcggcaaaa gccaaagcat cagtgccgca ctaatgagcc
    12361 acgcggggat ggtggtgtac atcatatttt tgatgtgctc aaacaagtcg atgccgacga
    12421 tggacgcgga aatacccgtc gtatccgaaa gcggggacat tttgtcgcca aaaaatgcgc
    12481 ccgaaacaat cgcgcccgcc gtcatcgcca tatcggcctg aaacgccgcc gccatcccca
    12541 taaaggcaac gccgacagtg gcgcaggtgg tcaggctgct gccgatggac acgccgatga
    12601 cggaacacag cgcgaaggcg gaaaaataaa aataagtcgg agaaatcagc ccgaaaccgt
    12661 aatacatcag cgtcggaatc gcaccgctca tcatcagtgc gctgaccatc agcccgatga
    12721 agaaaaacag gtaaatcgcg cccataccct gattcaacgc gcctatcatg ccctgctgca
    12781 tatcgttgta tttcaaaccg cgcgccaagc cgtacaaaat cagcacgacg atggcggcaa
    12841 taatggacat atgcggcagc cactccaatg aaatgatggt atagcccatc gcggcaatca
    12901 gagcgacgac gacggcaagt gcctcaccgc gcggcatatc gagtaaggat ttgaaagcaa
    12961 acatgattct ttgcccgatt tgatataaag ttaacggatt ctaacacact ataccgaatc
    13021 cgcccgacgt tttcagacgg cattttagcg ttttacaaaa gccggacaga aacaaaacgg
    13081 caattttctc aatttaaatt aatttttttg aaatataatt ttttattttt ttgtttgttg
    13141 gcatataaga cttttatgga ttgaaatatt aaaacctcgg cacacacgcc gcaaacaccg
    13201 gtacgcaaaa atgccgtctg aaagatgttc agacgacatt tctcaagaaa aagattattt
    13261 gtccgcccta tatccgtaca ccgcaaaaaa cacgatatac agataacaga tgacggaaac
    13321 gacaaacgaa gacatcaggg tgtaagtatc tgccacccag ccctgcacga caggaacgac
    13381 cgcaccgccg acaatcgcgg tacacagtac accggaagcc gcgttggtaa attttcccaa
    13441 tcctttggtt gccaaagaga aaatcgtcgg gaacataatc gagttgaaaa aaccgatggc
    13501 aagcagcgac cacatcgcca catccgcatt gccgctaccc gtcgccatcg cgatggcaag
    13561 cagtacgacc gcagccgatg cgttaaacgc caaataacgg ttgggcgcga atttcgccat
    13621 caccgccgaa ccgaggaaac gtccgaccat cgcgccgccc caatagaacg acaggtaatg
    13681 cgcggcagaa gcatgatcca gccctttcag ataacccaat acgttgacca tcaacgaacc
    13741 gatagacacc tccgcgccga catagcagaa aatgcctgcc gtaccgaaca cgagatgttt
    13801 gtattgccat acgccggttt tgccgtcgtg gttgtgcgcg ctttcctcgg cggcaatttt
    13861 gcgcgcgtcg ggcagccgga tcattttcac gaaaacggca aggataatca gcagccccgc
    13921 cagtcccaaa tagggaatct gtacggaaga aatctgttcc gccttgctga cggtttgggt
    13981 tgcgtccgcc aaaatcagga acgcgccgat ttgcggcgca atggtcgtac ccaaagcgtt
    14041 aaacgcctga accagcgtca gtgttgccga ttccttgccg ggtttcgcca gcagggtaac
    14101 ataaggatta ccggcaacct gaagcagcgt tacgccggaa gccaaaataa acaacgcgcc
    14161 caaaaatacc gcgtaagaat ggctgcccgc agcaggataa aacagcaggc atccgaccgc
    14221 cgtcagcaga aagccgccga taacgccgtt tttgtagccg actttgccga caaaagcccc
    14281 catcgggatg gacatcaccg cataggcggt aaagaaacag aattggatca gcatcgcctg
    14341 aacgtaagac aggtcgaaaa tttctttcaa atgagggata aggatgtcgt tcatgcaggt
    14401 aataaaaccc atcatgaaga acagcgtggt caagacgacc aatggggacg tatgattgtt
    14461 ttgtgattgc gcagacatga tttgcctttc gtggtgtcgg aatattaccc gtttcaagaa
    14521 aatagaaatt gacatttctt aacggcaatg caaatcatac tgatatttga acattttgca
    14581 aactttatcg gatgcaggca atgcgccgat ctgaaattca agcagcagaa attaatattt
    14641 tacagttgct tgcatagatt ttaatagatt ttaaaaaatt ccggcggact tgcaaaaacc
    14701 attcccaata aaatgaaaat ccgccgccgt atccggaaaa ttcggaaatg ccgcatcgtg
    14761 gcatcaaaac cggagcggaa agaatgtcgt atgcccgaca aaaaaggggc atacggctgt
    14821 gccgtatttt ttgcaccgtc ctccgccctt cggcgggggc gtgaaaatat agtggattaa
    14881 caaaaatcag gacaaggcga cg
//
LOCUS       DAWKST010000038        14892 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.038, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000038 DAWKST010000000
VERSION     DAWKST010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14892
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.038"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(104..1891)
                     /gene="lpdA"
                     /locus_tag="WJL87_000798"
     CDS             complement(104..1891)
                     /gene="lpdA"
                     /locus_tag="WJL87_000798"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ7799254.1"
                     /translation="MSLVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGAAASPKAEAPVAAPAQEAPKAA
                     APAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNV
                     GCIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKNGVVSRLTGGLAGMAK
                     GRKVDVIQGDGQFLDSHHLEVSLTAGDAYELAAPTGEKKIVAFKNCIIAAGSRVTKLP
                     FIPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQ
                     GADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLV
                     AAGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHE
                     GHVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASG
                     RAIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIH
                     PHPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1968..3569)
                     /gene="aceF"
                     /locus_tag="WJL87_000799"
     CDS             complement(1968..3569)
                     /gene="aceF"
                     /locus_tag="WJL87_000799"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ7799255.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPVA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGSAIIEVETAGSAAAAPAPAAQAAAPAPAAAPVPAAAAAPAPSAP
                     AAAKIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIMGDDIKAFVKSVMQGG
                     AAKPAAASASLGGGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVT
                     VHEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLV
                     LKNYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGAC
                     FTISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRV
                     IDGAAGMRFTVFLAKLLKDFRRITL"
     gene            complement(3719..6382)
                     /gene="aceE"
                     /locus_tag="WJL87_000800"
     CDS             complement(3719..6382)
                     /gene="aceE"
                     /locus_tag="WJL87_000800"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ7799256.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSKLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALSA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7076..8788
                     /locus_tag="WJL87_000801"
     CDS             7076..8788
                     /locus_tag="WJL87_000801"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ7799257.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERTAAQAELTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVESENEGEIVLLLLRGDHEFNDIKAEKLAGVKSPLTMASSAAIVEQFGANGG
                     SLGPVGFKGKVYADFATEKGADWVIGANEDGYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDAVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTA
                     SLNG"
     gene            8851..9507
                     /locus_tag="WJL87_000802"
     CDS             8851..9507
                     /locus_tag="WJL87_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEZ7799258.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGMGEGEDILGCVAGYGVGLDLTA
                     RDIQCHLKAQGLPWLKAKGFRHSACVSDFAAADRIGNPEKVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9517..9972)
                     /gene="ruvX"
                     /locus_tag="WJL87_000803"
     CDS             complement(9517..9972)
                     /gene="ruvX"
                     /locus_tag="WJL87_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ7799259.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(9965..10513)
                     /locus_tag="WJL87_000804"
     CDS             complement(9965..10513)
                     /locus_tag="WJL87_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ7799260.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10515..11075)
                     /locus_tag="WJL87_000805"
     CDS             complement(10515..11075)
                     /locus_tag="WJL87_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ7799261.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11403..13193
                     /locus_tag="WJL87_000806"
     CDS             11403..13193
                     /locus_tag="WJL87_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ7799262.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAVTNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAATKAQI
                     SRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQKALAV
                     QEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLEQKGNE
                     QQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRAEMSNL
                     TAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGVFYSTA
                     PATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAAGSKIG
                     SSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13313..14797
                     /locus_tag="WJL87_000807"
     CDS             13313..14797
                     /locus_tag="WJL87_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ7799263.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAEQAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLNGAVGVSAAFLPPEAVVVSTKGRDG
                     KDRMVLKAVPEDYVYGMGGDPLAGIPAELKTVPMTVLINSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
BASE COUNT         3533 a         3701 c         4199 g         3459 t
ORIGIN      
        1 ccggaagaaa tattttaatc cgctgtaaaa tagaaaatgc cgtctgaaca tttcagacgg
       61 catttgctca aataaacctt atcggctgtt tattcagtcg gatttatttt ttcttttgcg
      121 gaggcaggtc ggtacaagta cccaatgcca cttccgcagc cataccgatg gattcgccca
      181 aggtcgggtg cgggtggatg gttttgccga tgtctgccgc gtcgcagccc atttcgatgg
      241 caaggcagac ttcgccgatc atgtcgccac cgttcggacc gacaatgccg ccgccgatga
      301 tgcggccggt ttcggcatcg aaaatcagct tggtaaagcc gttgtcgcaa ccgttggcaa
      361 tcgcacggcc ggaagccgcc cacgggaagt tggctttggt gattttgcgg ccggatgctt
      421 tggcggacaa ctcggtttcg cccacccacg ccacttcggg ggaagtgtag gcaacgcccg
      481 gaatcacgcg cgcgtcgaag taggctttgt ggccggcgca gttttcggcg gcaacgtggc
      541 cttcgtgaac ggctttgtgc gccaacatcg gctgaccgac gatgtcgccg atggcgtaga
      601 tgtgcggcac attggtacgc atttgtttgt ccacttcgat gaagccgcga tcggttacgg
      661 caacgcctgc tttttccgcg ctgatgagtt tgccgttggg cgcgcggccg gcggcaacca
      721 atacggcatc gtagcgttgc ggctctttag gcgcatttgc accttcaaag gtaacgtaaa
      781 cgccgtcttc tttcggctcg actgcaacgg ttttggtgtt gaccataatg ttgtcaaaac
      841 ggtattcgtt ttgtttttgc catactttta ccaaatcgcg gtctgcgcct tgcatcaggc
      901 cgtccatcat ttcaaccaca tccaaacgcg aacccagcgt gctgtaaacc gtacccatct
      961 cgagaccgat aataccgccg ccgataatca gcagtttgcc cggtacttct ttcagagcca
     1021 atgcgccgct ggaatcgatg atgcgcggat cttcaggaat gaaaggcagt ttggtaacgc
     1081 ggctgcctgc tgcaatgata cagtttttga aggcaacgat ttttttctcg ccggtagggg
     1141 ctgccagttc gtacgcgtcg ccggcagtca gcgatacctc caagtggtgc gaatctaaga
     1201 attggccgtc gccttggata acatccactt tacggccttt cgccatacct gccaaaccgc
     1261 cggtgaggcg ggaaaccacg ccgtttttgt aggcgcgcag catatcgatg tcgagttcag
     1321 gctcggggta tttgataccg ttggcagcca agtggcgcac ttcgtcgata acggcggcat
     1381 tgtgcaacag ggctttggaa gggatacagc cgacgttcag gcaaacgccg cccaaggttt
     1441 tataacgttc gacgatggcg actttcaagc cttcatcggc agcagcaaat gcagcggagt
     1501 aaccgccggg accgccgccc aatacgacca cgtcgtactc ggcatcggca gaaccgccga
     1561 attgcgcggc ttgcggagcg ggagcggcag ctttgggtgc ttcttgcgca ggggcagcaa
     1621 caggtgcttc ggctttagga gaggcagccg cgccttcggc ttcaacgact acaatcaagc
     1681 cgccttcgga gattttgtcg ccgactttga ctttcacttc tttgaccacg cccgcaactt
     1741 cggcaggcac gtccatcgtc gctttgtcgg tttccaaggt aatcagggtg tcgtcaacgg
     1801 cgatggtatc gcccacattc acttcaaccg cgataatatc tacattttcg tgtccgccaa
     1861 tgtcgggcac tttcaattca actaagctca tgtctcatct ttctgaatga tgttcaaact
     1921 tccttttcag acggcacagg ccgctatgcc gtctgaagcc gatcgggtta cagggtaatg
     1981 cggcggaagt ctttcaacag cttcgccaag aatacggtga agcgcatacc ggccgcaccg
     2041 tcgatgacac ggtggtcgaa ggacaggctc aacgggcaca tcaggcgcgg ggcaaactct
     2101 ttgccgttcc aaacaggttt gatttgggat ttgcacacgc ccaagatggc gacttcggga
     2161 gcgttcacaa ttggcgtgaa gcctgtgccg ccgatgccgc ctaagctgga aatggtaaag
     2221 cacgcgcctt gcatttcttg cggtttgagc ttgccttcac gggctttttt ggacaattcg
     2281 gtcaattctt ggctgatttg tttcaagcct ttttgatcca cgtctttgat gacgggaaca
     2341 accaagccgt tcggcgtatc ggctgcgaaa ccgatgttga agtagttttt cagcaccagg
     2401 ttgtcgccgt ccagtgaggc gttgaattcg gggaatgctt tcaacgcgga aacagaggct
     2461 ttgatgatga acgccaacgg ggacagtttc acgccttcgc gttcccattc tttgttcagc
     2521 tgtttgcgga attcttccag ctcggtcatg tccgcttctt cgtgtacggt aacgtgggga
     2581 atcacaaccc agttgcggga caggttttga ccggaaattt tcttaatgcg ggacaattct
     2641 ttaacttcga cattgccgaa tttggagaag tccactttag gccacggcag taagtccaga
     2701 ccgccgccca aagatgcgct ggctgcggca ggttttgccg cgccgccctg catcacggat
     2761 ttcacaaagg ctttgatgtc gtcgcccatg atacggcctt tcaagccggt gcctttgact
     2821 tggcccaaat ccacgcccaa ttcgcgcgcc agtttgcgtg cggaaggacc ggcgtgtgct
     2881 ttggcgaaag cggcctcgtc gattttggca gcggcaggtg cagaaggtgc gggcgcggcg
     2941 gcagcagccg gaaccggagc ggcagccggt gcgggtgcgg cagcttgagc ggcaggcgca
     3001 ggggctgctg cggcagagcc ggcggtttct acttcgataa ttgcagagcc ttcggacact
     3061 ttgtcgccga cttttaagaa tacggcttta acgacaccgg cagcggtaca aggtacgtcc
     3121 attgtcgctt tgtcggtttc caaagtaatc agcgtgtcgt cttcggcaac ggtgtcgccc
     3181 actttgattt caacggcgat gacatccaca tcggtatggc cgccgatatc gggaacggct
     3241 acttgaacgg ttgcaccgcc tgcggcaacg ggtgcggcag caggtgcagg ttgtgcttcg
     3301 gcagcagcag gagcggcttc ggcagcggcg gcaccggttt caacggtcaa aattacgccg
     3361 ccttcggaga ttttgtcgcc gactttgact tttacttctt tcacgacacc ggccgcatcg
     3421 gcaggcacat ccatcgtggc tttgtcggtt tccagtgtaa tcagggtgtc gtcaacggcg
     3481 atggtgtcgc ccgctttaac ttctacggcg atgatgtcga cgttttcgtg accgccgata
     3541 tcggggactt tgatttctac gatactcatt tgagttcctt taatcttgtt cagttggatg
     3601 ccgtctgaaa gctgcttcag gcggcatcgg tacggtttgt ctgctcaaac cattcgggca
     3661 ttcggttttc agacggcctg atgtcggaat ggggcaaaca gaccgtctga aacatcaatc
     3721 agcgtttcca gctaggagct gaatcggctt tgatgccgta tttctcaatg gcttgttgaa
     3781 cggtttcttt gctgactttg ccttgttccg ccaatgcgct caatgctgcc acggcaacgt
     3841 tgtagcgatc cacttcaaag aagcggcgca ggttggcgcg gctgtcggag cggccgaagc
     3901 cgtcagtacc caagacgtgg tagtcgttcg ggatgtaggc gcggatacgg tcagcatagc
     3961 tgcggatata gtcggtagcg gcaataaccg gaccgtcatg accttgcaat ttggaagtaa
     4021 cgaaaggtac tttttcggct tccagcggat gcaggcggtt gaagcgttct acctcgacag
     4081 cgtcgcggtg cagcaggttg aaggacgggc aagaccaaat gtctgcttcc acgccgaagt
     4141 cggctttcag caattcggca ccggcaatga cttcttgcag gatggtaccg gagcccatca
     4201 attgaacttt cttatcgcct ttgccgccgg ctttcagcag gtacatacct ttcaagatgt
     4261 cttgttctgc gccttcgggc atatccggat gggtgtagtt ctcgttcatc agggtgatgt
     4321 agtagaacac gtcttcatta ttggcataca tacggcgcag accgtcttgt acgatgacgg
     4381 cgacttcgta ttggaaagtc gggtcataag atacgcagtt cggaatcagg tcggcctgga
     4441 tgtggctgtg gccgtcttcg tgttgcaggc cctcgccgtt cagcgtcgta cggccggcag
     4501 taccgcccag caggaagccg cgcgcgtgca tatcgcccgc cgcccaagcc aagtcgccga
     4561 tacgttggaa accgaacata gaatagtaaa tgtagaacgg aatcatcgcg aagttgctgt
     4621 tggcgtagct ggttgcagcc gcaatccagt cggccatcgc gcccggttcg ttaatacctt
     4681 cttgcaagat ttgaccgtca acggattctt tatagaacat cagttggtct ttgtcttgag
     4741 gggtatattg ctgacctttc ggattccaaa taccgtattg gcggaacata ccttccatgc
     4801 cgaaagtacg gctttcgtcg ggaacgatag gtacgacgcg tttgccgatt tttttgtctt
     4861 tcagcaaagt ggacaggatg cggacgaatg ccatcgtggt cgagaactcg cgttcaccgc
     4921 tggatttgag ttgtgcgtcg aatgctgaca gctcgggcac ttccaatact tcctgcgtcg
     4981 gtttgcgttg cggcaggtag ccgcccaaag catcgcggcg tgcgtgcagg tatttgtatt
     5041 cttccgtatc gggggcaaaa gtcaggtaag gcagatcgcc gctttcgatt tgttcgtcgg
     5101 taaccggaat gtcaaagcgg tcgcggaatt gtttcaggga cgctttgtcc atttttttgg
     5161 cttggtgggc aacgttctga ccttcgccgg atgcgcccat accgtaacct ttaatggttt
     5221 tcgccaagat gacggtaggt ttgccgtcgg catggttcgc tgcgcggtcg taggcgttgt
     5281 acactttttg cgggtcgtgg ccgccgcggt tcaatgccca gagttgctca tcggtcatat
     5341 cggcaaccaa tgctttcagt tcgggcgtat tgaagaagtg ttcgcgcaca tacgcgccgt
     5401 ctttggattt gtaagtttgg tagtcaccgt ccaaacattc ttccatacgt tggcgcagga
     5461 taccgtcttt gtctttcgcc aagaggcggt cccagcggcg gccccaaatg actttgacga
     5521 cattccagcc ggcgccggca aagttgcctt ccaattcttg gatgattttg ccgttgccgc
     5581 gtaccggacc gtccaagcgt tgcagattgc agttgatgac gaaaatcagg ttgtccaagc
     5641 cttcgcgtgc agccagtgcg attgcacctt gagattcggg ttcgtccatt tcgccgtcgc
     5701 cgcagaaaca ccatacttta cggcctttgg tttttgccaa accacgcgat tccaagtatt
     5761 tcaggaaacg cgcctgataa atcgccatga tgggccccaa gcccatggat acggtcggga
     5821 actgccaaaa gtcgggcaag aggtgggggt gcggatagga aggcagaccg tgtccgtcca
     5881 cttcttggcg gaagttgttc agctggtctt cggtcagacg gccctcgacg aatgcgcgtg
     5941 catagatgcc cggggcgacg tgaccttgga agaagaccaa atcgccttct tcgccttcgc
     6001 ctttggcttt ccaaaagtgg ttgaaaccga cttcgtacat ggtggcggca gattggaaag
     6061 atgcgatgtg cccacccagt tccaaatctt tcttgccggc gcgcaatacg atggcggcgg
     6121 cgttccagcg cacgaatgcg cggatgcggt gttcgatgtt ttggtcgccc ggaatgcctt
     6181 tttcgttttc aaccgaaacg gtattcaaat acggtgtggt cgtgccgtgt ggcatacgta
     6241 cgcccttgtc gcggcagtat ttgaccaggt tttccaagag gtattgcgcg cgttcgccgc
     6301 cttcatattc gaggacggag cttaacgcgt ccagccactc ttgggtttcg atagggtcaa
     6361 catcgtgtaa ttgggtggac atagtatcta tcctttatgt tgagtgttta atcggcaggg
     6421 agggtgtagc caaccccttt attttcgttt gtgaaaaata ataagcaaaa acgaaaattt
     6481 ctaaatcaaa ccgaaaaaaa taatttaagt ttaatgtttt caaaaaatta taaaagtttt
     6541 ggaaacaagg gaaaacttga aaaaaatgtt cgatctgctc cacacgaaat cctatcagca
     6601 atcgggtctg ctggcaaata accatgcctg taaaagcagg ttttatactg aagttggcgt
     6661 ttggtaaggt caagtctttt tgatttcgaa atattctgtt tgattttggc gatatttttt
     6721 cggaattgtg gtatatcgaa tcattatgtc tctgtcggca attttcctat tgtagaaaat
     6781 ctatacacaa gctttgtgga taaccgtgtt gataagccac ggcttgtgtt aaaaaagtct
     6841 tacgcaacaa atgtacgctt attttgatta aaaaataggc acatttaaat ctataaaaat
     6901 catgtgctta tgttcaatat gatgtttctg gtcatgtttt tgccgtctgt tatgggattt
     6961 gtcccgcgct tggggcaggg ggtaaatttt ggaggttggt gcaaaacctg cttttttcgc
     7021 gttacaatct gcacccgatt tacttttgaa tttgcctgtt tggaaaaata tttttatgaa
     7081 agccagccaa ttctttatct ctactttaaa agaagcccct gccgaagccg cgcttgccag
     7141 ccacaagctg atgatacgcg ccggtctgat taaagccaac gcgtccggcc tttatacttg
     7201 gatgccgatg gggctgcgcg tgttacgcaa agtcgaaaac gtcgtgcgcg aggaaatggc
     7261 gcgcgcgggc agcgtggagc tgctgatgcc ggtggtgcag cctgccgagc tgtggcagga
     7321 atccggccgc tgggaatttt acggtaaaga actgctgcgc ctgaaagacc gccacgaccg
     7381 cgatttctgc atgggaccga cctgcgagga agtcatcgcc gacatcgtgc gcaaagaaat
     7441 caacagctac aaacagctgc cgaaaaactt ttaccacatc caaaccaaat tccgcgatga
     7501 agtgcgcccg cgtttcggcg tgatgcgcgc gcgcgagttc gtcatgaaag acgcttattc
     7561 cttccatgcc gactacgcct cgcttcagac gacctatgat gccatgtatg acgcttactg
     7621 ccgcatcttt acccgtctgg gcttggcatt ccgccccgtc gccgcagaca ccggcagcat
     7681 cggcggtacg ggttcgcacg agtttcaagt gttggcggaa agcggcgaag atgttattgc
     7741 atacagcgac acttccgatt acgccgccaa tatcgagttg gcaccgacct tgccgcttaa
     7801 aggcgaacgc accgctgctc aggccgagtt gaccaaagta catacaccaa atgtcaaaac
     7861 cattgagtct ttggttgaat tcctgaatat tccggttgaa caaaccctca aatccatcgt
     7921 ggtcgaaagc gaaaacgaag gcgaaatcgt cttattactg ttgcgcggcg accatgagtt
     7981 caacgacatc aaagccgaaa aactcgcagg cgtgaaatcg ccattgacga tggcaagttc
     8041 tgccgcgatt gtcgaacagt tcggcgcaaa cggcggctct cttggtcctg tcggcttcaa
     8101 aggcaaagtc tatgccgatt tcgctaccga aaaaggcgcg gactgggtca tcggtgcgaa
     8161 cgaagacggc taccactata ccggcttcaa cttcggtcgc gatgccgccg agcctgaatt
     8221 cgtcgatttg cgtaatgtgg tcgaaggcga cgaaagcccc gacgggcaag gccgtctgaa
     8281 actggcgcgc ggcatcgaag tcggacacgt cttccagttg cgcgacaaat acacccaagc
     8341 catgaacgtg agcttcctcg acaacaacgg caaatcgcaa atcatggaaa tgggctgcta
     8401 cggtatcggc atcacccgcg tcgttgccgc cgccatcgag cagaacaacg acgaaaaagg
     8461 catcatctgg acgaaggcga tggcgccgtt tgaagtcgtt atcgtgccga tgaactacaa
     8521 aaaatcagac gccgtgcgcg aagccgccga caaaatctac gccgaactgc tggcggcagg
     8581 cgcagacgtc ctcctcgacg accgcgacga acgcgcaggc gtgttgctga acgattccga
     8641 gctgttgggt attccgcacc gcatcgtcat cggcgaccgc gctttgaaag aaggcaatgt
     8701 cgaatacgcc gaacgccgca acaacgaagc gcaggcagtt gcaatcggag aaattgttgc
     8761 gcgtgtaaca gcttcattaa atgggtaagc ttggtcaaat gctgtctgaa aataatttca
     8821 gacggcattt ttatttgggg agaaagtgaa gtggcttcag tatttttaga aggagaagca
     8881 gtcgaggtcg gcaatatttt ctgtatcggc aggaactatg ccgcacatat tgaagagtta
     8941 aaaaacgaaa taccttccga acctgtggtg tttatgaagc cgtcaggcag cattttgaat
     9001 agcggcggta caatcttgct gcccgagttt agtagggatg tgcagtttga gtgcgaactc
     9061 gtgttgctgg tcggtaagga ttcagacggc atgggagagg gtgaagatat tttgggatgt
     9121 gtggcggggt acggcgtggg tttggatttg accgcgcgag acatccaatg ccatctgaag
     9181 gcgcaggggc tgccctggct gaaggcaaaa gggttcaggc attccgcgtg cgtgtcggat
     9241 tttgcggcgg cagacagaat cggcaatccc gagaaggtgt tgttttcatt aaaacaaaac
     9301 ggcgtattga aacagcgcgg ggataccggt ttgatgattt acccgattcg ggaaatttta
     9361 cacaaactgg cggcggatta cggtttgggg aaaggggatt tggtgtttac cggaacgccc
     9421 tccggcgtgg gcgcaatcgg tgcgggggac aatttggcat tggagctgga cggcttggtg
     9481 cgcgcctctt tcacggtagg gtgttaaccg cgccgcttaa ccctcacgcc cgttgaaaca
     9541 ttccgccgga ccgccctcga aaaaaccgtg caagatggct tgcgccgcca cttggtcgag
     9601 caccgatttg cgttttttgc cgaagacctg tgcttccgaa agcaggcttt cggcatagac
     9661 ggacgacagc cgttcgtcaa cccaatagac ggggagattg aacctgccgt tcagcctgcg
     9721 tccgaacttg cgcgacaggt gcgtcatttc atgtttcgtg ccgtcggtat gcacgggcag
     9781 tccgacgaca aaataacgcg gctgccattc ttgaaccagc ttggcgattg ccgcgaactt
     9841 ttcatcgttg ctgccgccgg taacggtgct caaaggatgg gatagcccta attccgcgtc
     9901 tccttgtgcc acgccgatac gcgcttcgcc gaagtcgaat gccagtgccg ttccttttgg
     9961 aattttatgc atggccggct cccgaaaaca gggcgagcgg gtcgatgccg agtttggcga
    10021 atgcggcggc gtaacggtgt tcgtagggga tgtcgaacag gatgtgttcg tcggcgggaa
    10081 cagtcagcca cgcattgtcg gcaagttcgc gttcgagctg gccttttccc cagcttgaat
    10141 agcctatgct gatcaaggct ttgtcaaccg caccttcgcg tgaaatattt tcaatcacgt
    10201 ctcgggaaga agttagcgcg atattgtctg aaacgccgat actgctttgc cagttgccga
    10261 tcggggtatg cacgacataa cctcgctcga cttgcaccgg cccgcccatc atcacgctgt
    10321 cgtgctgcat ccgcatgggg atgtttttgc cggtggcgga aaaaatcatg tccatcgtaa
    10381 tcggagaggg tttgttgatg gcgatgccga gtgcgccgtc ttcatcgtgt ttgcagatat
    10441 agacgaccga ttgtgaaaaa aacgcgtctt ccatatcggg catggcgacg agaaagtggt
    10501 tggagaggtt catatcagcg tttgtcggga ttttctatcg ggttggaaat catgcaggcg
    10561 ccggcaagca ggttgccgga agtatcggct tgcaccccat cggtttgcgg tatgccgccg
    10621 ccgaaacagg aaaccgcgct taaggaattt ttcggcgagc cttggatgat tttatcgctg
    10681 tacaccaaat aggcgaaggt tttgcgtttg gggtcgtaat aacggacaat ctgccggctc
    10741 ttgaacgcga agctcgcacc gtgtttgaaa acttctttcg gtttgcgcac ggcggtttcg
    10801 tcaaaagaaa tcgaagatgc cgtctgaacg cacgaaaccg atgcgtcgga cgcgtcctct
    10861 tccaaattga ccatttcctt caagccgcct ttttttgcat acgaaatata acaggcaacc
    10921 ccttgaacgt cgggatcgtc gaatccttcc acttcgatac ggtcgttttt gcccagtatg
    10981 ttgaaaacgg tacttgcccg tccgatttta tcggtttcgc cgctgccgca ggcagtcagc
    11041 aggacggcgg cagacagcag tagcagtctg ttcatcaaaa tttccttatc ggttgaatcc
    11101 ccgttttccg tgaaggcggc agggcttgct ttgtttggag gggcgcacgt tcccaaaatc
    11161 gggcaacata cggtatgcgg cttaatgtgc taccctgtgt gtcggaacac aatatccgtt
    11221 gcggcattga aaatatgacg cacaatataa ggctgtttga gccgattgca agtatccgcg
    11281 caaagaaggc gcaagattgg gcaaacggca ttttcagacg gcataactga cagtataatc
    11341 caaacgtccg gctgctttcg tgcagccttc agtattatcc aactttttcc gaaggtcgaa
    11401 ccatgcgcta caaacccctt ctgcttgccc tgatgctcgt tttttccacg cccgccgttg
    11461 ccgcccacga cgcggcacac aaccgttccg ccgaagtgaa aaaacagacg aagaacaaaa
    11521 aagaacagcc cgaagcggcg gaaggcaaaa aagaaaaagg caaaaatggc gcagtgaaag
    11581 ataaaaaaac aggcggcaaa gaggcggcaa aagagggcaa agagtccaaa aaaaccgcca
    11641 aaaaccgcaa agaagcagag aaggaggcga catccaggca gtctgcgcgc aaaggacgcg
    11701 aaggggataa gaaatcgaag gcggaacaca aaaaggcaca tggcaagccc gtgtccggat
    11761 ccaaagaaaa aaacgcaaaa acacagcctg aaaacaaaca aggcaaaaaa gaggcaaaag
    11821 gacagggcaa tccgcgcaag ggcggcaagg cggaaaaaga cactgtttct gcaaataaaa
    11881 aagtccgttc cgacaagaac ggcaaagcag tgaaacagga caaaaaatac agggaagaga
    11941 aaaatgccaa aaccgattcc gacgaattga aagccgccgt tgccgctgtc accaatgatg
    12001 tcgaaaacaa aaaagccctg ctcaaacaaa gcgaaggaat gctgcttcat gtcagcaatt
    12061 ccctcaaaca gcttcaggaa gagcgtatcc gccaagagcg tatccgtcag gcgcgcggca
    12121 accttgcttc cgtcaaccgc aaacagcgcg aggcttggga caagttccaa aaactcaata
    12181 ccgagctgaa ccgtttgaaa acggaagtcg ccgctacgaa agcgcagatt tcccgtttcg
    12241 tatcggggaa ctataaaaac agccagccga atgcggttgc cctgttcctg aaaaacgccg
    12301 aaccgggtca gaaaaaccgc tttttgcgtt atacgcgtta cgtaaacgcc tccaatcggg
    12361 aagttgtcaa ggatttggaa aaacagcaga aggctttggc ggtacaagag cagaaaatca
    12421 acaatgagct tgcccgtttg aagaaaattc aggcaaacgt gcaatccctg ctgaaaaaac
    12481 agggtgtaac cgatgcggcg gaacagacgg aaagccgcag acagaatgcc aaaatcgcca
    12541 aagatgcccg aaaactgctg gaacagaaag ggaacgagca gcagctgaac aagctcttga
    12601 gcaatttgga gaagaaaaag gccgaacacc gcattcagga tgcggaagca aaaagaaaat
    12661 tggctgaagc cagactggcg gcagccgaaa aagccagaaa agaagcggcg cagcagaaag
    12721 ctgaagcacg acgtgcggaa atgtccaacc tgaccgccga agacaggaac atccaagcgc
    12781 cttcggttat gggtatcggc agtgccgacg gtttcagccg catgcaggga cgtttgaaaa
    12841 aaccggttga cggtgtgccg accggacttt tcgggcagaa ccggagcggc ggcgatgttt
    12901 ggaaaggcgt gttctattcc actgcaccgg caacggttga aagcattgcg ccgggaacgg
    12961 taagctatgc ggacgagttg gacggctacg gcaaagtggt cgtggtcgat cacggcgaga
    13021 actacatcag catctatgcc ggtttgagcg aaatttccgt cggcaagggt tatatggtcg
    13081 cggcaggaag caaaatcggc tcgagcgggt cgctgccgga cggggaagag gggctttacc
    13141 tgcaaatacg ttatcaaggt caggtattga acccttcgag ctggatacgt tgatcggatt
    13201 gccgaaacgg taggctttat gcaaatcgtg ccgatatggt gcgctgttcc gtgaagtttg
    13261 ccgtacctgt attttccggt ttttggaaat caagagagaa agttaaagaa gaatgtcgaa
    13321 acctgttttt aagaaaatcg cactttatac tttgggtgca atcagcggcg tggccgtcag
    13381 tctggcggtg cagggttttg ccgccgagaa ggaccggcgg gataacgaag tcctgccggt
    13441 gcaatccatc cgcacaatgg cggaggttta cggtcaaatc aaggcaaact actatcagga
    13501 caaacccgat gccgatttgt ttgaaggtgc gatgaagggt atggtggccg gtttggatcc
    13561 gcattccgaa tatatggata aaaaaggtta tgccgagata aaggagtcca ccagcggcga
    13621 atttggcggc ttggggatgg aaatcgggca ggaagacgga tttgtcaaag tggtttcgcc
    13681 gattgaggac acgcctgcgg aacaggcggg ggtgaaaagc ggcgatttca ttgtgaaaat
    13741 cgataatgtt tcgacacgcg gcatgacggt cagcgaagcg gtgaagaaaa tgcggggcaa
    13801 gccgggtacg aagattactt tgacgctgtc gcgcaaaaat gccgacaagc cgatagtcgt
    13861 caacctgacc cgtgccatta ttaaagtgaa aagcgtccgc catcacctga tcgaacccga
    13921 ttacggctat atccgcgtgt cgcagttcca agagcggacg gtcgaaagcg tcaataccgc
    13981 cgcaaaagag ctggtaaagg aaaataaagg aaaaccgctc aaggggctgg tgttggattt
    14041 gcgcgacgac cccggcgggc ttttgaacgg cgcggtcggc gtgtcggcgg cgtttttgcc
    14101 gcctgaagca gtcgtcgtca gcaccaaggg acgcgacggc aaagaccgca tggtactgaa
    14161 agccgttcct gaggattatg tgtacgggat gggcggcgat ccgttggcgg gcattcctgc
    14221 cgagttgaaa accgttccga tgacggtgtt gattaattcc ggttcggctt ccgcgtcgga
    14281 gattgtcgca ggtgcattgc aggatcacaa acgcgcggtc atcgtcggta cgcagagctt
    14341 cggcaaaggt tcggttcaga ctttgattcc tttgtccaac ggcagcgcgg tcaagctgac
    14401 aacggcactg tattatacgc cgaacgaccg ttctattcag gcgcagggga ttgttcccga
    14461 tgtcgaagta aaagataagg aacgcatttt tgaaagccgc gaggcggatt tggtcggaca
    14521 catcggcaat cctttgggcg gcgaggatgt gaacagtgaa acccttgccg tgccgcttga
    14581 aaaagatgcg gataagcccg ctgtaaaaga aaaaggtaaa aagaaaaagg acgaggattt
    14641 gtcttcaagg cggattccca accctgccaa agacgaccag ttgcggaaag ctttggattt
    14701 agtcaagtcg cccgagcagt ggcagaagtc tttggggctg gcggcgaaaa agccggtttc
    14761 aaataaagat aagaaagata aaaaagataa gaagtaggtt ttgacgatgc cgtctgaaag
    14821 gatttcagac ggcatattat gtttgaaagg acaggttatg agcgattttt taccggaatt
    14881 tgaaccgtgc gg
//
LOCUS       DAWKST010000039        14215 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.039, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000039 DAWKST010000000
VERSION     DAWKST010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14215
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.039"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(14..604)
                     /locus_tag="WJL87_000808"
     CDS             complement(14..604)
                     /locus_tag="WJL87_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ7799264.1"
                     /translation="MSERIILPVLSLGGVRLEPLDVHHEMGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
     gene            complement(665..1246)
                     /locus_tag="WJL87_000809"
     CDS             complement(665..1246)
                     /locus_tag="WJL87_000809"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ7799265.1"
                     /translation="MRNEEKHALRRELRGRRSQMGWDVRAAATVKINRLLKRYIKHGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     ARLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVDRLSVEAHDLPLDGFVSEAGILCF"
     gene            1324..1533
                     /locus_tag="WJL87_000810"
     CDS             1324..1533
                     /locus_tag="WJL87_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799266.1"
                     /translation="MWHIVAIGYLFVAVMFSAAQPSIARTLIYLVFWAVLPTVFTVFV
                     VTIRRRNRLMKQQEQAEFEQQRAQR"
     gene            1734..2462
                     /gene="rpsB"
                     /locus_tag="WJL87_000811"
     CDS             1734..2462
                     /gene="rpsB"
                     /locus_tag="WJL87_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ7799267.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDHRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            2591..3445
                     /locus_tag="WJL87_000812"
     CDS             2591..3445
                     /locus_tag="WJL87_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ7799268.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            3662..4381
                     /locus_tag="WJL87_000813"
     CDS             3662..4381
                     /locus_tag="WJL87_000813"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ7799269.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDSSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
     gene            4565..5527
                     /gene="mafA"
                     /locus_tag="WJL87_000814"
     CDS             4565..5527
                     /gene="mafA"
                     /locus_tag="WJL87_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ7799270.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSKSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVETDNSHEGYGYSDEVVRQHRQGQP"
     gene            5567..6949
                     /gene="mafB"
                     /locus_tag="WJL87_000815"
     CDS             5567..6949
                     /gene="mafB"
                     /locus_tag="WJL87_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ7799271.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGDGRGSVKNRVYAVQTFDATAVGPILPITHERTGFEGVIGYETHFSGHGHE
                     VHSPFDNHDSRSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNW
                     WANRMDDVRGIVQGAVNPFLMGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLS
                     PEAQLAAATALQDSAFAVKDGINSAKQWADAHPNITATAQTALSVAEAAGTVWGGKKV
                     ELNPTKWDWVKNTDYKTPAARPMQTLDGEMAGGNKPVVKSIRPTTRDELRQALQEQGF
                     RRTGSDAAQYETWKGPDGVKIDIRPNGEVIRTQRVPRTDGVQGKYPQRQDYEGNPLPN
                     NHHHSGYFVK"
     gene            6946..7263
                     /locus_tag="WJL87_000816"
     CDS             6946..7263
                     /locus_tag="WJL87_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799272.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            7333..8001
                     /gene="mafB"
                     /locus_tag="WJL87_000817"
                     /pseudo
     CDS             7333..8001
                     /gene="mafB"
                     /locus_tag="WJL87_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            8011..8217
                     /locus_tag="WJL87_000818"
     CDS             8011..8217
                     /locus_tag="WJL87_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799273.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            8349..8600
                     /locus_tag="WJL87_000819"
     CDS             8349..8600
                     /locus_tag="WJL87_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799274.1"
                     /translation="MDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQT
                     WGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            8597..8968
                     /locus_tag="WJL87_000820"
     CDS             8597..8968
                     /locus_tag="WJL87_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ7799275.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            9054..9179
                     /locus_tag="WJL87_000821"
                     /pseudo
     CDS             9054..9179
                     /locus_tag="WJL87_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            9219..10334
                     /gene="mafB"
                     /locus_tag="WJL87_000822"
                     /pseudo
     CDS             9219..10334
                     /gene="mafB"
                     /locus_tag="WJL87_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            10404..10634
                     /locus_tag="WJL87_000823"
     CDS             10404..10634
                     /locus_tag="WJL87_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HEZ7799276.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEI
                     HYIENTRTGEKTDFKFIENTRTGEKTDFKFKD"
     gene            10637..10960
                     /locus_tag="WJL87_000824"
     CDS             10637..10960
                     /locus_tag="WJL87_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799277.1"
                     /translation="MFLDDVNVFLDDLNTNPITDEWYMSNFADKHIKILESYEAFDIL
                     KQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQEISQDIL
                     NEIFR"
     gene            10998..11342
                     /locus_tag="WJL87_000825"
     CDS             10998..11342
                     /locus_tag="WJL87_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799278.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            11702..11902
                     /locus_tag="WJL87_000826"
     CDS             11702..11902
                     /locus_tag="WJL87_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799279.1"
                     /translation="MASDLLSKQTSDKILGYQTEHRRVRYDINNNIYVLANPKTFKIK
                     TMFKPNLGKKYYDGEFKKDMGN"
     gene            11868..12074
                     /locus_tag="WJL87_000827"
     CDS             11868..12074
                     /locus_tag="WJL87_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799280.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKDLPIR"
     gene            12095..12313
                     /locus_tag="WJL87_000828"
     CDS             12095..12313
                     /locus_tag="WJL87_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799281.1"
                     /translation="MMTESHGYLSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYCWLGFDELPQSEKIKFLSYLNIL"
     gene            12310..12669
                     /locus_tag="WJL87_000829"
     CDS             12310..12669
                     /locus_tag="WJL87_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ7799282.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            12745..12957
                     /locus_tag="WJL87_000830"
     CDS             12745..12957
                     /locus_tag="WJL87_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799283.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
     gene            13044..13160
                     /locus_tag="WJL87_000831"
                     /pseudo
     CDS             13044..13160
                     /locus_tag="WJL87_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            13222..13578
                     /locus_tag="WJL87_000832"
     CDS             13222..13578
                     /locus_tag="WJL87_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ7799284.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITESQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            13751..14125
                     /locus_tag="WJL87_000833"
                     /pseudo
     CDS             13751..14125
                     /locus_tag="WJL87_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4547 a         3032 c         3024 g         3612 t
ORIGIN      
        1 tgaaaatact gcctcacacc tgcacgccat accctgccaa cctgccggtc aggatttccc
       61 tgtttttgca ccaatcttcc ctcagcatac tgtacacgac cgtatcgcgc acactgccgt
      121 ctttacggag catatgcata cgcagcacgc cgtctttttc cgcacccagc cgttcgatgg
      181 cacgttgcga ggcaaggttc agaatatccg tgcgccatcc cacgcaacgg caagccaaaa
      241 catcaaatgc gtaatccaac agcatgattt tgcaacaggt gtttatccgt gtccgccgtg
      301 ccgatgccgc ataccatgtg aatccgatat ccaaacgcgg aatctgcggt tcaaaatgat
      361 aatacgccgt tgtcccgacc accctgcccg cctcttcatc gacaaccgca aacgccaaac
      421 gcgttgccaa tgctgtcccg atatagtctg ccaccctatc cggatggggc gcggacgtta
      481 ctcccagctt ccaaacttcc ccatcgcaaa ccgcctcgcg caaacccatt tcatgatgca
      541 catccaacgg ttcgagacga acgccgccca acgacaagac cggcagtatt atcctttccg
      601 acatcctttt ctcccaatat tccgccttca gacggcattt ccgttcaaaa tgccgtctga
      661 acggctaaaa acacaatatc cccgcctccg acacaaaacc gtccagcggc aggtcgtgcg
      721 cctcgaccga cagtctgtcc accaactggc aggcaaagcc cacgcccacg gtttttgcct
      781 gcaaacggta tttcatcgct gcaagcgtcg catcgtaata gccgcctgcc tgccccaaac
      841 ggtagccttc gcggtctatg ccgacaagcg gaacgagcaa taccgacaaa ccgtgcacgc
      901 ggattttgcg ccctgcaaac tgcggcacgt tcaacctcgc cctgccccgt atgcgctccc
      961 gttccattcc gctttcagga tacggcgtaa accacatccg ccgcgaacgc ggttcgatat
     1021 aaggcagata aagttttgcg ccgcgttttt gcgccgcgcg gacaaagccg tccaaacgca
     1081 attccttgcc catcggccaa tacacgccga ttttccgacc gtgcttgata taacgtttga
     1141 gcaggcggtt aatttttacc gttgccgccg cccgcacgtc ccaccccatt tgcgaacgcc
     1201 gcccgcgtaa ttcgcggcgc agggcgtgtt tttcctcgtt cctcatttca gacggccttt
     1261 caggattgcg gtagaatgtt gcgattataa cgattttgtt aacattcaaa caggacgcac
     1321 acaatgtggc acattgtcgc catcggctat ctttttgttg ccgtgatgtt ttccgccgcg
     1381 cagccgagta ttgcgcgcac cttgatttat ttggtttttt gggcggtgct gcccaccgtg
     1441 ttcacggtct ttgtcgtgac catccgccgc cgcaaccgcc tgatgaagca acaggaacag
     1501 gcagaattcg aacagcagcg tgcacaacgg taaaaagaca gcggcacaaa accctgaatc
     1561 ccttttcaga cgacctctta tccgctataa tccgtcagtt ttccatttcg gaaacactct
     1621 tttatttttt aaaacttatg cccactttcg ccgaagggtg cttgacaata ggcgcggtct
     1681 atcaagttct atgcgattga atgtgtgccc ttaacccttt caaggaaatc aaaatgtctc
     1741 aaattactat gcgtcagatg attgaagccg gtgtccactt cggccaccaa acccgtttct
     1801 ggaacccgaa aatggctcaa tacattttcg gtgcgcgcaa caaaatccat atcgtcaacc
     1861 tggaaaaaac cctgccgatg ttccaagacg cgcaagaagc cgtacgtcgt ctggttgcca
     1921 acaaaggtac agtattgttc gtaggtacca aacgccaagc ccgcgacatc atccgcgaag
     1981 aagcgacccg cgccggcatg cctttcgtcg atcaccgttg gttgggcggt atgctgacca
     2041 actacaaaac cgttaagcaa tccatcaaac gcctggaaga aaaaaccgca gccttggaaa
     2101 atgctgccga aagcggtttc agcaaaaaag aaattctgga aatgcaacgc gatgttgaaa
     2161 aactggaacg ttctttgggc ggtatcaaaa acatgaaagg cctgcccgac gcgattttcg
     2221 ttatcgatac cggctaccaa aaaggtactc tggtcgaggc tgaaaaactg ggcatccccg
     2281 ttatcgccgt agtcgatacc aacaacagcc ctgacggcgt gaaatacgtc atccccggca
     2341 acgacgactc cgccaaagcc atccgcctgt actgccgcgg catcgctgac gcagttttgg
     2401 aaggcaaaaa ccaagcgctg caagaaaccg tagccgctgc ccaagaagcc gctgccgagt
     2461 aatccggcaa accgaagagg ggcgttatgc cccttttctc aaatatgccg tctgaacgtc
     2521 cgttcgcggc acacgattcc cgaatgcgga aaatcctttc cgtatttccc aaaaatctag
     2581 gagattcaaa atggcagaaa ttactgcaaa aatggttgcc gacctgcgcg ctgctaccgg
     2641 cctgggcatg atggaatgca aaaaagcctt ggttgaagcc gaaggcaact tcgacaaagc
     2701 cgaagaaatc ctgcgtatta aatctggtgc gaaagccggt aaactggctg gccgtactgc
     2761 tgccgaaggc gtattggctt acgcgatcaa cggcaatgtc ggcgcattgg tcgaagtaaa
     2821 ctgcgaaacc gacttcgttg ctaaagacgc gggcttcgta gaatttgcca acttcgttgc
     2881 gaaaactgcc gccgagaaaa aaccggcttc tgttgaagaa ctgagcgaac tggttgaagc
     2941 agaacgcaaa gccatcatcg ccaaattggg cgagaacatg tctgtccgtc gcttccaagt
     3001 gatcgacact gccaaccaac tggttgccta catccacggc gcattggcga ccgaaggcgt
     3061 attggttgag tacaaaggct ctgaagacgt agcacgcaaa atcggtatgc acattgttgc
     3121 cgctaaacca caatgcgtaa gcgaagccga agtagatgcc gaaaccgttg aaaaagaacg
     3181 ccacatctac accgaacaag ccatcgcttc cggcaaacct gccgacatcg ccgctaaaat
     3241 ggttgaaggc cgtattcgca aattcttggc tgaaatcact ctgaacggcc aagcattcgt
     3301 gatgaacccc gatcaaactg ttgcccaatt cgccaaagaa aacggcactg aagtggtcag
     3361 cttcgtccgc tacaaagtag gcgacggtat cgagaaagcc gttgtcgact acgcagccga
     3421 agtagccgca gccgccaaag tgtaaggcac ttatgaaaaa gaaagcacct ggattccaaa
     3481 cgaatcaggg tgcttttttt tgagaaaacc gtttgcggta cctattttaa gacgaccgaa
     3541 tattcagacc gtcttaaaac aaaacaataa taaaccgaca caccctatca ttaatattcc
     3601 gaccgtttga aattcagacg acccaactcc gaccgacgac attcaagaaa gcaaggtatc
     3661 catgacacag caaatcaaat acaaacgcgt attactgaaa ctctccggcg aatccctgat
     3721 gggttccgat ccgttcggca tcaatcacga taccatcgtt caaactgtcg gcgaaattgc
     3781 cgaagtcgtt aaaatgggcg tgcaagtcgg tattgttgtc ggcggcggca atattttccg
     3841 gggcgtatcc gcccaagcag gcagcatgga tcgcgccacc gccgactaca tgggcatgat
     3901 ggcgaccgtg atgaacgcgt tggcactcaa agacgcattt gaaactttag gcatcaaagc
     3961 gcgcgtacaa tccgcactgt ctatgcagca aatcgctgaa acctacgccc gccccaaagc
     4021 catccaatat ttggaagaag gcaaagtcgt gatttttgcc gccggtaccg gtaacccgtt
     4081 cttcacgacc gacactgccg ccgcattgcg cggtgcggaa atgaactgcg acgtgatgct
     4141 caaagccacc aacgttgacg gcgtgtacac cgcagacccc aaaaaagact catccgccac
     4201 gcgctacgaa accattactt ttgacgaagc cttgttgaaa aacctcaaag tgatggatgc
     4261 caccgctttc gccctctgcc gcgaacgcaa gctcaatatt gtcgtattcg gcattgctaa
     4321 agaaggctcg ctcaaacgtg tagtaaccgg tgaaaacgaa ggtacgctgg ttcactgctg
     4381 attgaccata gtgtcggcag atatagtcgc atatgggctt cagacagcca tttattatat
     4441 ggagattata gtggattaaa tttaaaccag tacggcgttg cctcgccttg ccgtactggt
     4501 ttaaatttaa tccactatat ttacaatttt gatacaattt gtttttcatc aaaggagaaa
     4561 atctatgcaa gcacggctgc tgatacctat tcttttttca gtttttattt tatccgcctg
     4621 cgggacactg acaggtattc catcgcatgg cggaggtaaa cgctttgcgg tcgaacaaga
     4681 acttgtggcc gcttctgcca gagctgccgt taaagacatg gatttacagg cattacacgg
     4741 acgaaaagtt gcattgtaca ttgcaactat gggcgaccaa ggttcaggca gtttgacagg
     4801 gggtcgctac tccattgatg cactgattcg tggcgaatac ataaacagcc ctgccgtccg
     4861 taccgattac acctatccac gttacgaaac caccgctgaa acaacatcag gcggtttgac
     4921 aggtttaacc acttctttat ctacacttaa tgcccctgca ctctctcgca cccaatcaga
     4981 cggtagcgga agtaaaagca gtctgggctt aaatattggc gggatggggg attatcgaaa
     5041 tgaaaccttg acgactaacc cgcgcgacac tgcctttctt tcccacttgg tacagaccgt
     5101 atttttcctg cgcggcatag acgttgtttc tcctgccaat gccgatacag atgtgtttat
     5161 taacatcgac gtattcggaa cgatacgcaa cagaaccgaa atgcacctat acaatgccga
     5221 aacactgaaa gcccaaacaa aactggaata tttcgcagta gacagaacca ataaaaaatt
     5281 gctcatcaaa cccaaaacca atgcgtttga agctgcctat aaagaaaatt acgcattgtg
     5341 gatggggccg tataaagtaa gcaaaggaat taaaccgacg gaaggattaa tggtcgattt
     5401 ctccgatatc cgaccatacg gcaatcatac gggtaactcc gccccatccg tagagactga
     5461 taacagtcat gaggggtatg gatacagcga tgaagtagtg cgacaacata gacaagggca
     5521 accttgattc acactaccat aaccgcttgc taccaaggaa aacaaaatga atttgcctat
     5581 tcaaaaattc atgatgctgt ttgcagcagc aatatcgttg ctgcaaatcc ccattagtca
     5641 tgcgaacggt ttggatgccc gtttgcgcga tgatatgcag gcaaaacact acgaaccggg
     5701 tggcaaatac catctgttcg gcgatggtcg cggcagtgtt aaaaatcggg tttacgccgt
     5761 ccaaacattt gatgcaactg cggtcggccc catactgcct attacacacg aacggacagg
     5821 atttgaaggt gttatcggct atgaaaccca tttttcagga catggacatg aagtgcacag
     5881 tccgttcgat aatcatgatt caagaagcac ttctgatttc agcggcggtg tagacggtgg
     5941 ttttactgtt taccaacttc atcggacagg gtcggaaatc catccggagg atggatatga
     6001 cggaccgcaa ggcagcgatt atccgccccc cggaggagca agggatatat acagctatta
     6061 tgtcaaagga acttcaacaa aaacaaagac taatattgtc cctcaagccc cattttcaga
     6121 ccgttggcta aaagaaaatg ccggtgccgc ctctggtttt ttcagccgtg cggatgaagc
     6181 aggaaaactg atatgggaaa gcgaccccaa taaaaattgg tgggctaacc gtatggatga
     6241 tgttcgcggc atcgtccaag gtgcggttaa tcctttttta atgggttttc agggattggg
     6301 agttggggca attacagaca gtgcggtaaa cccggtaacc tatgcggcag cacggaaaac
     6361 tttacagggt attcacaatt taggaaattt aagtccggaa gcacaacttg cggctgcaac
     6421 cgcattacaa gacagtgctt ttgcggtaaa agacggtatc aactctgcca aacaatgggc
     6481 tgatgcccat ccgaatataa cagcaacagc ccaaactgcc ctttccgtag cagaggccgc
     6541 aggtacggtt tggggcggta aaaaagtaga acttaacccg actaaatggg attgggttaa
     6601 aaataccgat tataaaacac ctgctgcccg acctatgcag actttagatg gggaaatggc
     6661 tggtggaaac aagccagttg ttaaatctat cagaccaact acgcgagatg aattacgtca
     6721 agcattgcaa gaacaaggtt ttagacgtac tggttcagat gcggctcaat atgaaacatg
     6781 gaaaggtcct gatggcgtga aaatagatat tcgtccaaat ggagaggtta taagaaccca
     6841 aagagtgccg cgaaccgatg gtgtacaggg aaaatatccg caacgacaag attatgaagg
     6901 caatccattg ccaaataatc atcatcattc tggatatttt gtcaaatgaa aaaaaatatt
     6961 tttcacaatg taagccttta tgaaataatc ttttccgata atggaaatac ccttacatta
     7021 tcttttacag atacaattga aggtaattat ttcggatata tcaaatgcag taatattttg
     7081 aattttaaat tagatacaaa taatttcgta gattatgagg ataaggaaga tagcttgttt
     7141 cccttgttta tacccgaaat agagctatat aaataccaat tttatagtga aattattatt
     7201 gatgtaggga ttattataaa aatatctgct gaaacaatta attttgagcc actgggaaaa
     7261 tagtaactgc tttctcagca gccgtagcaa ctgtattttt acccgacggg gtaaaaatac
     7321 agttgctaca tctctgcgca gcagactcta caaggtatta ataatttagg aaatttaagt
     7381 ccagaaacac aacttgctgc cgcgagccta ttacaggata gtgcttttgc ggtaaaagac
     7441 ggcattaatt ccgccagaca atgggctgat gcccatccga atataactgc aacagcccaa
     7501 actgcccttg ccgtagcaga ggccgcaact acggtttggg gcggtaaaaa agtagaactt
     7561 aacccgacca aatgggattg ggttaaaaat accggctata aaaaacctgc tgcccgccat
     7621 atgcagactg tagatgggga gatggcaggg gggaatagac cgcctaaatc tataacgtcc
     7681 aacagcaaag cagatgcttc cacacaaccg tctttacaag cgcaactaat tggagaacaa
     7741 attagtagtg ggcatgctta taacaagcat gtcataagac aacaagaatt tacggattta
     7801 aatatcaatt caccagcaga ttttgctcgg catattgaaa atattgttag ccatccaaca
     7861 aatatgaaag agttacctcg cggtagaact gcgtattggg atgataaaac agggacaata
     7921 gttatccgag ataaaaattc tgacgatgga ggtacagcat ttagaccaac atcaggtaaa
     7981 aaatattatg atgatttata ggaaaaagcc atgaatatac tatccataaa taatcaaaac
     8041 tcaactattt cactaactca agatgaagtt tttgttttac gagctatctt gaatgagata
     8101 tatgcgggcg tatgtgtaga ttcaagagaa tttgaaaatg tatctggtgt tagaaaacat
     8161 gaagtagata atttacaaca acagtttgct ggaatttata aaaaaatgac aacttaacaa
     8221 cccaaatttt tgtcagagcc tagtgcaaat tacaactatg attctattgt agccgaaatg
     8281 aaagaaaaaa atcatgggtt ggcgacaggg ttgatgttgt taatatgcct gatggagcac
     8341 ctactagtat ggataacacg cgtattatgg cagcacgtga agcaggggta aaagtggaag
     8401 cgaatgttca taattttaat gaccgattat catcaaaaga gagaatcagg tttaagcatg
     8461 atggtattga gcctcaaact tggggagaag ctatccagct acgaattaga aagcaagaaa
     8521 cacaaaaagg agttccagaa gggtggagca aaagatttcc taacggaagt atttatgatg
     8581 taaaggtact taggaaatga taaaacaaaa tagttttgtt ccgtatcctg aagcaatgct
     8641 tcctaaagga tttaaatatc cgcaaagtta tttaaaatta gctcaatcca ctcatgccat
     8701 taactacgat gaacaatatt cttttccttg gtggtttgaa aatgcagaaa gcaatatatc
     8761 agaagtaatt gacatttatt ttgaaataac tggcattcca aacctattac cttttgctag
     8821 aaaccaagag tgggctgcct gttttgatat ttcagataaa tcaggtaatc ctaaaattat
     8881 agtagttaat ttagataata caaaatatta cgagactttt gaaaattttg atacttggct
     8941 aaaagaagct gaaaatgatg gttggtagca gccgtaggtc ggattctcga atccgacatt
     9001 ttccaacagc ggcatttcgg aaacgataga cgcgtcaaat atttttgtcg gatacaagta
     9061 tccgaccata cggcaatcat acgggtaact ctgccccatc cgtagaggct gataacagtc
     9121 atgaggggta tggatacagc gatgaagtag tgcgacaaca tagacaagga caaccttgat
     9181 tcacactacc ataaccgctt gctaccaagg aaaacaaaat gaatttgcct attcaaaaat
     9241 tcatgatgct gtttgcagca gcaatatcgt tgctgcaaat ccccattagt catgcgaacg
     9301 gtttggatgc ccgtttgcgc gatgatatgc aggcaaaaca ctacgaaccg ggtggtaaat
     9361 accatctgtt tggtaatgct cgcggcagtg ttaaaaagcg ggtttacgcc gtccagacat
     9421 ttgatgcaac tgcggtcagt cctgtactgc ctattacaca cgaacggaca gggtttgaag
     9481 gtgttatcgg ttatgaaacc catttttcag ggcacggaca tgaagtacac agtccgttcg
     9541 atcatcatga ttcaaaaagc acttctgatt tcagcggcgg tgtagacggc ggttttactg
     9601 tttaccaact tcatcgaaca gggtcggaaa ttcatccgga ggatggatat gacggaccgc
     9661 aaggcagcga ttatccgccc cccggaggag caagggatat atacagttac catatcaaag
     9721 gaacttcaac aaaaacaaag ataaacactg ttccgcaagc cccgttttca gaccgctggc
     9781 taaaagaaaa tgccggtgcc gcttccggtt ttctcagccg tgcggatgaa gcaggaaaac
     9841 tgatatggga aaacgacccc aatcaaaatt ggtggggtaa ccgtatggat gatgttcgcg
     9901 gcatcatcca aggtgcagcc aatccttttc taacgggttt tcagggattg ggagttgggg
     9961 caattacaga cagtgcggta aacccggtaa cctatgcggc agcacggaaa actttacagg
    10021 gtattcacaa tttaggaaat ttaagtccgg aagcacaact tgcggccgca accgcattac
    10081 aagacagtgc ttttgcggta aaagacggta tcaactctgc caaacaatgg gctgatgccc
    10141 atccgaacat tacagctact gcccaaaccg cccttgccat agcagaggcc gcaactacgg
    10201 tttgaggcgg taaaaaagta aaccttaacc cgaccaagtg ggattgggtt aaaaataccg
    10261 gctataaaaa acctgctgtt cgccatatgc agactataga cggggaaatg gctgggggaa
    10321 acaaatcatt aaaaataggg acacaatctg ttgaaaaatc aaccggtcgt acaataccta
    10381 ataatttaaa ggaacaatta gcaatggaag aagttaaggc aaacccacag ggcaaaactc
    10441 ctgcgagaat acctcctatg tccgatacta aaaatggttg gttagcaaaa gacggttggg
    10501 ttaagcgtgt tcaaaacgta aacaaaattg aaatacatta cattgaaaac acaagaaccg
    10561 gtgaaaaaac agattttaag tttattgaaa acacaagaac cggtgaaaaa acagatttta
    10621 agtttaagga ttagtcatgt ttttagatga tgtaaatgtt tttttagatg atttaaatac
    10681 taatccaatc actgacgaat ggtatatgtc caattttgcc gataaacata ttaaaatttt
    10741 ggaaagttac gaagcctttg atattctaaa acaatttgtt gattacatga ttgaagaata
    10801 tgatgaaaaa tcagaatatg aaatcatgga aatattgaga caattaaaat atcaagcaga
    10861 taccaacgaa aaattttata caaatacaca gaaacagaaa attgtagaat tatataaaca
    10921 agaaattagt caggatattt taaatgaaat ctttagataa actatcaata tagaaggaaa
    10981 tccttggaaa aaataaaatg ataatcgaac acaatggaaa tatacataaa atagccagaa
    11041 tgactggaaa taaaaataat tttttagaaa taatcctatc agatattcat gaaaacataa
    11101 aaatcaaacc attaactata aaagtaaaag gagagaatgt tataaatatc cttcctgagg
    11161 aagttagttt ttatgtaaaa caaggtgttg atttaattta tgaaaaatat aaacggaaat
    11221 tctttatctc cgaaatttct ttttgccaat cagatagccg gccttcaagt atctacgctt
    11281 ttcttacatt tcacttgctt gaagatatta ttaaaaatga atccccatcc aactacacct
    11341 gactggaggt acggtttggg gcggtaaaaa agtagaactt aacccgacca aatgggattg
    11401 ggttaaaaat accggctata aaacacctac tgcccgccct atgcagactt tagatgggga
    11461 gatggcaggt gggaataagc caccaaaacc aagtacgcag caacacccta cacactctga
    11521 taacaatatc ggcttacctg cctcatatgt taaacctgat acatctattt ctccgacagg
    11581 aacaattcaa gaccgcatca gatggacaaa gtccaagttt cctactgaga aatctttaaa
    11641 tggacatttc aaagctcatg gaaaagaatt tggcgatata accattgaag actaccaaaa
    11701 aatggcgtct gatttgttat caaaacagac atcggacaag atattaggtt atcagacgga
    11761 acatagacga gtgcgctatg atatcaataa caatatctat gttttggcca atccaaaaac
    11821 attcaaaatc aaaacaatgt ttaaaccaaa cttaggaaag aagtattatg atggagaatt
    11881 caaaaaagac atgggaaatt gacggagaaa tatggctaca ttgtcctgtt tgcggaactg
    11941 aagttatgga ctatgatatc tgtgacgttt gtcagtggca aaatacagga gaaactaata
    12001 tagatggtgg ccctaatgaa atgacacttg cggaggcgaa agaagcttac gcaaaagact
    12061 taccaatcag ataaataagc acctagagaa atcaatgatg acggaatccc atggttacct
    12121 atcaaataat ttacctgtta aaatcataaa tgatattatt tatgcaaccc agttagtcga
    12181 agatttggtt ttaggaaaaa taaaaattgt tgatttttta aaatcatata acaattttta
    12241 ttgttggctt ggttttgatg agttgcctca atctgagaaa ataaaattcc taagctatct
    12301 caatatatta tgagattaaa attaaaaaat tatgattgtt ctgactttga tttggaagat
    12361 ttcccacaag acaagttaga aaatttttgt atacttttga cattatcaat aggttttgat
    12421 gaaagtaatg gtgctgatta tttttatgtt tatatatata gcaccgaatg gttactttca
    12481 aatatacata gaccgatgtc attaaaaaac tcaattgtta caaaccgttt taatattgaa
    12541 cacatattaa aattgataaa tgatatattg gaaatatgta attcaacgtc tgaagataag
    12601 tctatatcaa atttggcaaa atattttgac tgggaatttg acgattataa tctaaatatc
    12661 caagactaaa gatggtggac agcatttaga ccaaaaacag gcaaacggta ttttgatgac
    12721 gaattaaaat aaaaggaatt attcatgaaa ttaattaatt ttgaagagag ttcagttaaa
    12781 attagtctcg ataaagatga gttgtatatt attcaagcaa ttgtaggaga aatttattca
    12841 ggcgtatgcg tggattgtag agattttgag ataattcatg gtgttgaaaa aataaagttc
    12901 tttctttgga taaagaactt aaaaaaatat atgatacttg ggataaatgt tagctaggta
    12961 aggtgggcac ttgttgccca ccttttgccg tatattaaaa atccttttca ggctggaact
    13021 gggtgggcat caatgccaac cctaattgtg ctgatgaagc aggaaaactg atatgggaaa
    13081 acgaccccaa taaaaattgg tgggctaacc gtatggatga tattcgcggc atcatccaag
    13141 gtgcggttaa tcctttaatt tacaaggtaa gcaagcaaaa gattatttac aacaacaaac
    13201 tcatattaag aacttagaca aatgaatgaa cacaacctgt taattttctg tttaaaagac
    13261 aatgtttcaa ttagtgaata tactgaaatg attgattggg cttataaaaa cattcaatct
    13321 gaaacagttg tagaaattac ggaaagtcaa attattgaat atcaaaatcg tggattatgg
    13381 agacttgttt ctgaaattac cgataattgg ttatttggac caagtgaggg ggattggcta
    13441 atagataagg aaagtatttt ggctgtaaaa gaaaaattac aaaattcaga tttttctaca
    13501 gagcccttag tgaaaaatat tattcatgta cttgaatatg ctataaaaaa tgaaaaaaca
    13561 gtaatttttc atttttgaga ctaatccaat ttttagtaat attgatgcag agcaagcagc
    13621 attagatgcc gcaaacatgg ggagaagcta ttcaatttag aattaaaaaa caaattgaaa
    13681 atgaactagc accaccaaat tggtctaccc agtttcctaa tggtagtatt tatgatccta
    13741 aggtaacgaa atgattattc aaaatgaatt taatttatat cctagtaata tgcttcctga
    13801 aaggttttgt tatcctgaaa agtatgttcg tatctctaac gatacatctt taatacctta
    13861 tattcagcca cataattttc actggtagtt tgagaattat ggaacagaag gggcagaagt
    13921 agcttatata tttagaaatt ctatcctgcc tgatttaaat cttatcccat tcgctagtaa
    13981 tggagaatgg gaagcttatt ttgatggtaa tgatgtaaca ggaaattcta gggttattgt
    14041 cattaattta gataatatag aaaaccatga attttttaat agttttgaag attggcttga
    14101 attagcaatt aaggatactt ggtaagcagc tatctataaa gagatgaggc tgccctggac
    14161 aactaggata aactcgattt tactaattgt tttaaaatgg aacaagaact tttat
//
LOCUS       DAWKST010000040        14079 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.040, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000040 DAWKST010000000
VERSION     DAWKST010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14079
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.040"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(53..361)
                     /locus_tag="WJL87_000834"
     CDS             complement(53..361)
                     /locus_tag="WJL87_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799285.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHDHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKELLADLDKELC
                     "
     gene            complement(345..647)
                     /locus_tag="WJL87_000835"
     CDS             complement(345..647)
                     /locus_tag="WJL87_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ7799286.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(721..3084)
                     /locus_tag="WJL87_000836"
     CDS             complement(721..3084)
                     /locus_tag="WJL87_000836"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ7799287.1"
                     /translation="MQFSYSWLKTQADTELSADKLEHLLTMSGLEVEEAETAAPAFTG
                     VVIAEVKSVEKHPDADRLNVTQVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSDGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQINAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAMERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLDRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAAHGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSDGRFVQNERIGGLWYGAAMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3139..4164)
                     /locus_tag="WJL87_000837"
     CDS             complement(3139..4164)
                     /locus_tag="WJL87_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ7799288.1"
                     /translation="MVSAIDSLKKNVNYNYVNSSNTHTIQIVHVEKPEGPIKIKRGDT
                     ETSISKQAIWRLADALSTGEPVNVDRVFGASYNFRSALEALLAHTPEIYWCKLQRIQP
                     GLNKSEVVEGHKHIIWLPDRPHEKGVMKEYETDVVISEIPSNNVVYDALSLPSTHSEI
                     DIDVKRRHVQIQIALSAIGFQLGFRTWIARNDQGISYGDKKIADLDGIVQKIESEKLI
                     SSFDGAVQAAMNIDCIWFRNGKLMPAVMEVEHSTGVRSGLARMKQLKDLLPPYANTRW
                     VIVAPDEDRDKVFKEANVPMFKNLDTQYFPYSAVEELYSLCIRRKLTNKAVNEEFLDC
                     FMEKCVM"
     gene            complement(4166..5260)
                     /locus_tag="WJL87_000838"
     CDS             complement(4166..5260)
                     /locus_tag="WJL87_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ7799289.1"
                     /translation="MIFSFFSGAGFLDLGFELSGFDIAFVNEVHPPFLEAYKYSRSRM
                     DIPKPKYGYFKGSIDECLYAEKAKDLAGWVKKEKQNGIIVGFIGGPPCPDFSIAGKNK
                     GKDGENGKLSQSYVDLICKNQPDFFVFENVKGLYRTAKHREFFNALKRQLSDFGYVCT
                     EKLINAIEYGVPQDRERIILVGFLSQHVDALQKFDWDAHISFPDALEKDWPTTEEVGR
                     VVSQPANIYPELTVQYWFNRNGVDTHPNASKHFQPRAGLEKFQTISEGDDKKKSYKRL
                     HRWRYSPTAAYGNNEVHIHPYLPRRISAAEALAIQSLPKEFELPDNMTLSNMFKTIGN
                     GVPFLAAKGIAMTLKSYLENHYERTKTDGC"
     gene            complement(5331..5777)
                     /locus_tag="WJL87_000839"
     CDS             complement(5331..5777)
                     /locus_tag="WJL87_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HEZ7799290.1"
                     /translation="MDRLTPEQRKKCMQSNKSKGTKPELALAKAMWALGLRYRKNSGS
                     IFGKPDFSFKKYKVAVFVDGEFWHGKDWEQRKAEIKGNREFWIAKIERNIRRDMEVTG
                     RLKVEGWAVLRFWSNDVVKNTTCCAEKVRQAVRDKQGLSKFQTREK"
     gene            complement(6101..7093)
                     /gene="pheS"
                     /locus_tag="WJL87_000840"
     CDS             complement(6101..7093)
                     /gene="pheS"
                     /locus_tag="WJL87_000840"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ7799291.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSGEIDIMGENGKWLEVGGCGM
                     VHPNVLRNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFAK"
     gene            complement(7439..7798)
                     /gene="rplT"
                     /locus_tag="WJL87_000841"
     CDS             complement(7439..7798)
                     /gene="rplT"
                     /locus_tag="WJL87_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ7799292.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKSALAA"
     gene            complement(7811..8008)
                     /gene="rpmI"
                     /locus_tag="WJL87_000842"
     CDS             complement(7811..8008)
                     /gene="rpmI"
                     /locus_tag="WJL87_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ7799293.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(8155..8676)
                     /gene="infC"
                     /locus_tag="WJL87_000843"
     CDS             complement(8155..8676)
                     /gene="infC"
                     /locus_tag="WJL87_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ7799294.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(8694..10607)
                     /gene="thrS"
                     /locus_tag="WJL87_000844"
     CDS             complement(8694..10607)
                     /gene="thrS"
                     /locus_tag="WJL87_000844"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ7799295.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGVGLARAAVAGRVNGKL
                     VDACDPIVENSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVAVKLSLRPEQRAGSDEVWDKAEQGLRDALTACGVE
                     WEELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(10660..10736)
                     /locus_tag="WJL87_000845"
     tRNA            complement(10660..10736)
                     /locus_tag="WJL87_000845"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10700..10702),aa:Val,seq:gac)
     gene            10915..12030
                     /gene="tgt"
                     /locus_tag="WJL87_000846"
     CDS             10915..12030
                     /gene="tgt"
                     /locus_tag="WJL87_000846"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ7799296.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQAKKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEQFDFPGLAVGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRTGEILGAQLNTIH
                     NLHFYQVIMAEMREAVEQGKFADWQARFHENRARGTD"
     gene            12040..13122
                     /locus_tag="WJL87_000847"
     CDS             12040..13122
                     /locus_tag="WJL87_000847"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ7799297.1"
                     /translation="MPSEAASDGILPLYPTRTIFQKPIMLPFFPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAKRMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHHDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            13314..13715
                     /locus_tag="WJL87_000848"
     CDS             13314..13715
                     /locus_tag="WJL87_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ7799298.1"
                     /translation="MNTTRLPTALVLGCLCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKN"
     gene            complement(13778..14068)
                     /locus_tag="WJL87_000849"
     CDS             complement(13778..14068)
                     /locus_tag="WJL87_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799299.1"
                     /translation="MAKYFTETGISVREYFDFFGEFVVLPAARSGDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGDGNIGSMGLKVPR"
BASE COUNT         3475 a         3800 c         3189 g         3615 t
ORIGIN      
        1 attttttagc gtgtaaaaca aaaccggctg catacctgca accggcctca aatcagcaca
       61 attccttatc caaatccgcc aacagctctt tcagaccgtc tccgatttcc tgaagcgtaa
      121 ccggacggtt gccgtccgaa cccgattccg caccttctgc atacggtgca aacgtgctct
      181 gcaatttcaa cagtttcacc acatccaaac gggtgtagcg ttcgccgccg taaccgacaa
      241 ccacaccgtg atcatgctgc cattgcgcaa aaccataggg gctgatttgc aacagtccgc
      301 acaactcgtc cagcgtgaaa tagcgttttg cgggaacctt caaatcaacg ttgtttgtca
      361 tagtagtgtt ccaccatgct tttgagtttc tggctggcat ggaaagttac cacgcggcgg
      421 gcggtaatcg gcacttcctc gccggtttta gggttacgac ccgggcgttg cggcttgtcg
      481 cgcaactgga aatttccgaa accggaaatt ttgatttctt cgccgcttgc caaagtgctg
      541 cggatttctt caaaaaagag ttcgacgatt tctttggcat cgtttttggt gacgttgctg
      601 actttgtcta ccaaaatatc ggccagttct gctttagtga gagtcatatg attaccttct
      661 gttgtaacag ttgtggatta ttaccaattt ttatttaaaa ttcaagcgaa tatttatttt
      721 ttaactgcga agccgcgccc ctgccgccgt tgccgcgcca atcagttttc cgataagcgg
      781 ctcgaccgcc tcatccgtca gcgtgttttc catatcctgc aaaatcactt tgaccgccac
      841 gctcttcatc ccttcgggca gtcccgtgcc gcgatacacg tcaaacacgc tgatttcctg
      901 taccaacttg tttgccgcgc ctttcaagac aagcagcaaa tcatcatggc tcatagcttc
      961 cggcatcaca aacgccaaat cgcggcgcac cggctggaat ttcgatacgg cccgatagcg
     1021 cgttttcccg cattccaaca cggccgccat atcgatttca aataccagcg gcgcttgcgg
     1081 caggtcgtat ttttgcagcc atttcggatg cagttcgccg acaaagccga tgactttgcc
     1141 gtctgaaacg atattggcgg cacgtccggg atgcagggcg ggatgtccgg ttttaacgaa
     1201 ctcgactgct ttgtttttca acagattttc cacatctgcc ttaatgtcgt aaaaatccgc
     1261 attgcgcgtt ttcccacccc attgttcggg cattgccgcg ccgtaccaca gaccgccgat
     1321 gcgttcgttt tggacaaacc ggccgtctga acctttgccg aacacgcggg cgatttcaaa
     1381 cacgcgcacg cggttttgtt tgcggttcag gttgttttgc aaaatttcca ccaagccgcc
     1441 gatgagtgtg gaacgcatca cggcatattg cgccgccagc gggttttgca ggcggatggg
     1501 gtcggcgttg gcggcaaaat cttgttccca ctgctcgtca acgaaggcat agctgaccac
     1561 ttcgcggtag ccgtgcgccg ccatttcgtt gtaaacggca aaacgcgggc ggcgtgtttc
     1621 gggcagttcc agcattttca gacggcctga cgtgtaatcg tcggggatgt tttcatagcc
     1681 gtaaacgcgt ccgatttctt caatcaaatc ggcctcaatt tcgatgtcaa aacggaagct
     1741 cggcgcggta acgcggaagc cttccgccgt tttctcgggc tgcaggccca agtgttgcaa
     1801 aatggtttcc acctgttcgg caggaatgtc cacgcccaac acggttttca gacggtccaa
     1861 acgcagtccg acctgcttgg cttcgggcaa ttcgccttgc gcttccacca tctcgcctgc
     1921 cgcaccgccg caaatctgca acaccaattc ggtagcacgt tccatggcat ccgcctgcaa
     1981 acggtaatcc acgccgcgct cgaagcggaa cgacgaatcc gaaccgaaac cgtattggcg
     2041 cgatttgccg gcgatgattt cgggcgcaaa ccaagccgct tccagcacga tattttgcgt
     2101 gccgtctgaa accgcgctcg ccgcgccgcc cattaagccc gccaaactca acacgccttt
     2161 ttcgtccgcc acgaccagcg tgttttcaga cagggaaacg gttttctcgt tcaggcattc
     2221 cagcgtttcc ccttcgcgcg cgcggcggat gtgcaggctg ccggaaagtt tgtcggcatc
     2281 aaaaacgtgc atcggctgac cgatttccag catcacataa ttgccgatgt ccaccagcgc
     2341 ggaaatactg cggatgccgc tgcgctccaa acgttgtttc atccaatcgg gagtagccgc
     2401 tttcgcattg acattttcaa taacgcggct gataaaacgg ccgcaatcgg caggcgcgtt
     2461 aatctgcacg ggctgttttc gactgcccgt gatcggcgcg gtatggattt cgggctgcct
     2521 gaacgcgcac cccgtcaatg cggacacttc gcgcgcaatg cctttgatgc tcaagcagtc
     2581 ggcgcggtta ggcgtaattt tcaacgtaaa cagcgtatcg tccaaatcca agtattcgcg
     2641 gatattggta ccgacgggcg catcttcagg cagaatgtgc aggccgttca caccgtcgtc
     2701 gggcagaccg agttcgtcgg tggaacacaa catcccgtcc gacaccacgc cgcgcatttt
     2761 ggtcggcttg attttgaaat tacccggcaa aacggcaccc ggcagcgaac acggcacttt
     2821 gatgcccgct ttcacattcg gcgcaccgca cacgatctga accaacccac ccgtacccgc
     2881 atcgacttgg gtaacgttca aacggtcggc atcaggatgt ttttcaacgg atttcacttc
     2941 ggcaattacc acacccgtaa acgcaggcgc ggcagtctcg gcttcttcca cttccaagcc
     3001 ggacatcgtt aacaggtgtt ccagcttatc ggcggaaagt tcggtatcgg cttgggtttt
     3061 cagccatgag taggagaatt gcatattttt cctaaggatt aaatttgatt ggacaactta
     3121 agggtaaccg agtgtaaatt acattacaca tttttccata aagcagtcta aaaattcttc
     3181 attgacagct ttatttgtca atttccggcg aatgcacaag gaatacaact cttcaactgc
     3241 ggaataagga aaatattggg tatctaaatt tttaaacatc ggaacattcg cttctttaaa
     3301 aactttatcc ctatcttcat caggagcaac gataacccag cgcgtattag catatggtgg
     3361 caacaggtct ttcaactgct tcatccgtgc aaggccggaa cgtacacccg tactatgttc
     3421 cacttccatc accgccggca tcagttttcc attcctgaac cagatgcaat caatattcat
     3481 tgccgcctga accgcgccgt caaacgaact gatcagtttc tcgctttcta tcttttgtac
     3541 gataccatcc aaatcagcaa ttttcttatc cccataacta ataccttggt cgtttctcgc
     3601 aatccacgtc ctgaaaccaa gttgaaaacc tattgctgaa agggcaatct ggatttgcac
     3661 gtggcggcgt tttacatcta tatctatttc tgaatgggtt gaaggaaggg ataaagcatc
     3721 gtaaaccaca ttgttggagg ggatttccga aataacgaca tctgtctcat attccttcat
     3781 tacgcctttt tcatgtggcc tatcgggaag ccaaataatg tgcttgtgcc cttccaccac
     3841 ctcgctttta ttcagacccg gttgtatccg ctgcaatttg caccagtaaa tttcaggtgt
     3901 atgggcaagc aatgcctcca gtgcggaacg aaagttataa cttgcgccaa acaccctgtc
     3961 cacattgaca ggctcccccg tggacaacgc atctgccaac cgccaaattg cttgtttcga
     4021 tatcgaagtt tccgtatctc cccttttaat cttaatcgga ccttccggtt tttcaacatg
     4081 gacgatttgt atggtatgcg tatttgagct gttcacatag ttataattga catttttttt
     4141 caagctatca atcgcactga ccaaattagc agccgtcagt ttttgtccgc tcatagtgat
     4201 tctccaaata agattttaat gtcatcgcta tcccttttgc tgccaaaaac ggtacaccgt
     4261 tgcctatagt tttgaacata ttgctcaatg tcatgttatc aggcagctca aactccttcg
     4321 gcaaagactg tattgccaat gcctccgcag cggaaatcct tcttggcaga tagggatgaa
     4381 tgtggacttc gttgtttccg tatgcagcag tcggagaata acgccagcgg tgtaggcgtt
     4441 tataggactt ctttttatca tccccttcgc ttatggtttg aaatttttct aaacccgctc
     4501 ttggttggaa gtgtttgctc gcattcgggt gtgtatccac cccattgcga ttgaaccaat
     4561 actgaacggt caactcaggg tatatgtttg caggctgact gaccactctt cctacttctt
     4621 ctgtcgtagg ccaatctttt tccaaggcat cagggaatga aatatgagca tcccaatcaa
     4681 acttctgcaa agcatcaaca tgctgactca aaaaacccac taggataatc ctttccctgt
     4741 cttgcggtac gccgtattcg atggcattga tgagtttttc agtgcataca tatccaaaat
     4801 cagaaagttg ccgtttcaac gcattgaaaa actccctatg cttggcagtc ctatatagtc
     4861 ctttcacatt ctcaaataca aaaaaatcag gctggttttt acaaattaaa tccacataac
     4921 tttgtgataa cttaccattc tccccatcct tacctttatt tttccctgca atagaaaaat
     4981 ccggacatgg gggacctcca ataaatccga caataatgcc attttgtttt tcctttttaa
     5041 cccagccagc caaatctttt gctttctcag cataaagaca ttcgtcaata ctacccttaa
     5101 aatatccgta tttaggcttc gggatatcca ttctgcttcg agagtattta tatgcttcca
     5161 aaaatggggg atggacttca ttaacgaagg caatatcaaa tcctgacaac tcgaagccca
     5221 aatccaaaaa gcccgcacca gagaaaaaag aaaaaatcaa agtttttttt ctttcctgcc
     5281 gtatgcttat atccaaattg cagcttttat ccgtcaactg gaaggggaga ttatttttct
     5341 ctcgtttgaa attttgacaa tccctgtttg tcgcggacgg cttgtctgac tttttcggca
     5401 cagcaggttg tatttttaac gacatcatta ctccaaaaac gtaaaaccgc ccagccctcg
     5461 accttcagac ggcctgttac ttccatatcc cttcggatat tgcgttctat cttggcaatc
     5521 caaaattcgc ggttgccctt tatttccgcc tttctctgtt cccaatcctt gccgtgccaa
     5581 aactcgccat caacaaacac ggcaacttta tattttttaa atgaaaaatc aggcttccca
     5641 aaaatgcttc cgctattttt ccgatacctg agccccaaag cccacatcgc cttcgccaac
     5701 gcaagctcgg gcttcgtccc cttgcttttg ttggactgca tacatttttt gcgctgttcg
     5761 ggggttaatc tgtccatttc gtgaggcctg aaacgcgttt atacgaaagc gcattcattt
     5821 tacgcggcat cgttgcggat tccggcagtt ttgcacccct tgcaaggaaa caacaaaacc
     5881 caatcgcgca ggaagccacc tgtttcgtca ggttttccct gcaaagccgc aaggttcgca
     5941 gccggacgga ctcaaaatta ccaagcatcc catccgcaca aatcttcaga acgcatcaat
     6001 tcatctgccg aaatcccgcc gaaatcttgc cgcaggcttt ttcagcccgt ccggccggac
     6061 aatatgcggg ttgcactccg cttgcaggct gcccgcacca ttatttcgca aactgcttca
     6121 aaaaattcaa atcgttatca aaaaacaagc gcaaatcgtt cacgttgtaa cgcagcatcg
     6181 cgaaacggtc gagaccgata ccgaaagcga aaccggtgta tttttcggga tcgatattca
     6241 catttctcaa cacgttggga tgcaccatgc cgcagccgcc gacttccagc catttgccgt
     6301 tttcgcccat aatgtcgatt tcgccagacg gttcggtgaa cgggaagaaa gacgggcgga
     6361 aacgtacttg caaatcatcg cgttcaaaga agcgacggat aaaatccgtg aacactgctt
     6421 ttaagtcggc aaaagttaca ccctcttcta cccacaaacc ttccgcctga tggaacatag
     6481 gcgagtgcgt ggcatcgctg tccacacggt aaacgcggcc gggggcgata atgcggatgg
     6541 gcggctcttt tttatcgagc atatagcgga tttgaatcgg ggaagtgtgc gtacgcaaaa
     6601 catcaccgtt ttcaacgtaa aacgtatcct gcatcgcacg ggcaggatgg tttgcaggga
     6661 tgttcagggc ttggaaattg tgaaaatcgt cttcgatttc aggcccgtcc gccacttcga
     6721 aacccattcc gtgaaagagt tcgaccacac gttgcaaggt cagggttacg ggatgcaggc
     6781 tgccgccttc ctgagcgcgt ccgggcaggg taatgtcgag ggcttcggcg gcaagtcggg
     6841 cttgcagctt ggcttcgttg agggcatcgc gtttggcatt aaaagccgtc tgaaaccggt
     6901 ttttgcattc attgatatgc gcacctatgg ttttgcgctc ttcaggcgac atttgcccca
     6961 aagttttcag aagtccggtc aactcgccgg ttttgccaag ataacgggct ttgatttgtt
     7021 ctagagcgtt gaagtcttgc gcagcttcta ctgcggcaat gccttctgca acgatgcggt
     7081 ttacattttc cataatatca agtctgtcaa ttaatgatat gccacctgaa acaacgaggc
     7141 cgcctgtata gcctgacata tcagactctt cagacgacct tattttcttc acggtcaatc
     7201 cattggataa attttctgta tagtggatta aatttaaacc agtacggcgt tgcctcgcct
     7261 tgccgtacta tctgtactgt ctgcggcttc gtcgctttgt cctgatttaa atttaatcca
     7321 ctatatttta acgcaaattt atttaaaaaa atagacttta aagaaagaaa atcaatcaca
     7381 tagaatttct gcaaacaaag aaaaaaggaa gccgcagctt ccttttcaat tttttggatt
     7441 aagcagccaa agcagatttg gctttttcaa ccaattgtgc aaaagcggct ttatcgaaca
     7501 cggccaaatc agccaatact ttgcggtcga tttcaataga ggcgcgtttc agaccgttca
     7561 taaatttgct gtaagacaac ccgttttcac gcgcacctgc attgatacgg acaatccaca
     7621 attgacggaa ttggcgtttg cgttggcggc ggtcgcggta tgcgtattgg ccggctttca
     7681 ttaccgcctg cttggcaacg cggtaaacgt ttttacgacg accgcggtag cctttggcta
     7741 acgcgaagat tttttggtga cgggcacggg cggtaacacc gcgttttacg cgtggcatat
     7801 tctaaactcc ttaagcgtag ggtaacattt tagcaacaga agccaaatcg cgatcattta
     7861 ccatagaggt accgcgcagt tggcgtttgt ttttggtggt ctttttagtc aagatgtggc
     7921 gtttgaacgc atgagcgcgt ttcacaccgc cgttacccag tactttaaag cgttttttcg
     7981 cgctagactt ggttttcatt ttaggcatgg gaaaactcca ttcgttatcg gataaggcat
     8041 tagggggttt taaaacatcg gtttcaaaca cttgaaccca caatgccacg gttttttgcc
     8101 gcaattaaaa acttactccg aagcggcgat cggagtaagc ggagaattat agctttattt
     8161 tttcttcggc gcaatcatca tcaccatttg gcgaccttcc attttgggaa aggactcgat
     8221 ttgcgccact tcagccaaat cttcttttac acgttccaaa agttgcgcgc cgagttgctg
     8281 gtgagccatt tcacggccgc gaaaacgcaa tgtcactttg actttatcgc cgtcggcaag
     8341 gaagcggtta atgttgcgca tcttgatttg ataatcgccc tcatccgtac ccggacggaa
     8401 tttgatttcc ttgatttgca cctgcttttg atttttcttg gcttcgtcgc gcttcttggc
     8461 ttgttggtat ttgtatttac cgtaatccat cagtttgcac acaggcggtt tagcagttgg
     8521 ggaaatctct accaaatcga catcctgccc ttcggccata gccaaagctt cacgaactga
     8581 aacgacacca agctgttcgc ctgactcact gattaaacgc acttctttgg cggtaatttc
     8641 gccgttgatt cgtgcttcgc gttcttgagc gatgatgaat actcctataa aaattaatga
     8701 ttgacgaggg catcagtgat ttcttgctgc aattgcgcaa tgaaatcatc caaatccaaa
     8761 gaacccaaat cttctgcttt gcggcgtacc gccactttgt tttcctgctt ttctttatcg
     8821 ccgacaacga tttgataagg aaaacggtat tggctgttgt cgcggatttt gtaaccgatt
     8881 ttttcgttac gcaaatccaa ctcggcgcgg aatcctgccg cctgcaattt ggcagccact
     8941 tcacggcagt aatccgcttg attttcggtg atattcataa tcaccatttg aaccggcgcc
     9001 agccataacg ggaaagagcc tgcatggttc tcaatcagaa tgccgataaa ccgctccaaa
     9061 gaacccaaaa tggcgcggtg caacattaca gggcgagcgc ggtcattgtt ttcggttaca
     9121 tattctgcat ccaagcgttc cggtaagacg aaatctagtt gcaatgtacc gcattgccaa
     9181 gaacgaccca aggcatcttt gacgtgatat tcgattttag gcccgtaaaa cgcaccttcg
     9241 ccaggcaact cttcccactc tacgccacaa gcggtcaatg catcacgcaa gccttgttcg
     9301 gccttatccc aaacttcgtc agaacctgca cgttgttcag ggcggaggga caacttcaca
     9361 gcaacatcat ggaaaccgaa ctgtttgtag atgcgaacca acaattcatt gaatgcgcgc
     9421 gcttcgctga cgatttggtc ttcggtacag aaaatatgcg catcatcttg cacaaaaccg
     9481 cgaacacgca tcagaccatg caatgcaccg cttggttcat tgcggtggca agaaccgaac
     9541 tcggccaaac gcatcggcaa atcgcgatat gagcgcaaac cgttattaaa aatttgcaca
     9601 tgacccggac agttcatcgg tttcaccgcg tattcgcgtt tttctgaact ggtcacgaac
     9661 atattgtctt tgtagttgtc ccaatggccg gatttttccc aaaaggtttt atccataatt
     9721 tgaggcgttt tgacctcttt ataaccggcg gcgttcagct ctttacgcat atgctgttca
     9781 atcacttgcc acaaagccca gcctttagga tgccaaaaca ccatgcccgg cgcttcgtct
     9841 tgcagatgga acagatccaa ttgcttgcca agtttgcggt ggtcgcgctt ttcggcttct
     9901 tcgatacgtt gaatataggc ttttaattcg tcttttgtcg cccaagccgt accgtatatg
     9961 cgttgcagca tttcattatt gctgtcgccg cgccagtatg cgcccgccaa cttggtcagt
    10021 ttgaagtttt tcaggaaacg ggtattcgga acgtgcggtc cgcggcacat atcgacatat
    10081 tcctgatgat gatacatccc catcgcttcc acttcgggca tatcgtcaat caggcgcagt
    10141 ttgtattctt cgccgcgctc ttgaaaaatt ttaatcgcct ccgcacgcgg agtcatgatt
    10201 ttgaccacat catagtcttg ggcaatcaat tctttcatac gcgcttcaat ggcggcaaca
    10261 tcttccggtg taaacggttt ttccgtggcg atgtcgtaat aaaagccctc ttcaatgacg
    10321 gggccgataa ccatttttgc attaggatag agttgcttga cggcatgccc gacaagatgg
    10381 gcgcaggaat ggcggatgat ttcgatgcct tcctgatctt tcggagtaat gatttgaaca
    10441 gcagaatttt caacaatcgg gtcgcacgca tcgaccaatt tgccgtttac cctgcctgcc
    10501 accgccgccc tcgccaaacc gacaccgata gacgcagcaa tttgagccac ggtaacgggg
    10561 gattcgtatt ggcggactga gccgtccggc aaggtaatgt tcaacatcaa acgctccgaa
    10621 agattaaaaa ataacggcat aaatgccgtt atgggaattt ggtaggcacg attggattcg
    10681 aaccaacgac ccccaccatg tcaaggtggt gctctaacca actgagctac gtgccttcaa
    10741 agaatttttg cattttatcg ggtcgtttta atttttgcaa gaggtatgtg catttcttta
    10801 tgcaacattg tggcaagatt tggggatgtc gtctgaaaaa ccgtgggaat tcatttataa
    10861 tgccgtcttt taaaacatat ttcgacccct gtcgcccaac tgacattcaa tcttatgctt
    10921 aaatttacct tacacaaaaa agacggatac gcccgacgcg gcacgctgga gttgaaccac
    10981 ggcaaaatcg aaacgccggt atttatgcct gtcggtactt acggctcggt taaggcgatg
    11041 aacccgcaaa acctgcacga catcaaggcg caaattattt tgggcaacac ttatcatttg
    11101 tggctgcgtc cgggcttgga ggtcgtcgaa cagtttggag ggcttcacgg ctttatcggc
    11161 tgggacaaac cgattctgac cgactcgggc ggttttcagg ttttttcttt gtcggatatg
    11221 cgcaagctga cggaagaagg ctgtacgttc aaaagcccga tcaacggcga caagctgttt
    11281 ttatcgcctg aaatttccat gaaaatccaa acggttttga actcggacat cgcgatgcag
    11341 ctggacgaat gcacgccggg cgaagcaacg cgcgaacagg cgaaaaaatc gctgcaaatg
    11401 agcctgcgct gggcggaacg gagcaaaaaa gcctttgaag atttgaaaaa cccgaacgcg
    11461 ctgttcggca tcgtgcaagg cgcgatgtat gaggatttgc gcgaagaatc gctgcgcggt
    11521 ttggaacagt ttgattttcc cggccttgcc gtcggcggtt tgtccgtcgg cgaacccaag
    11581 cccgaaatgt accgtatgct gcgcgccgtc ggtccgattc tgccggaaca caagccgcat
    11641 tacctgatgg gcgtaggcac gccggaagac ctcgtgtacg gcgtggcaca cggcatcgat
    11701 atgttcgact gcgtgatgcc cacccgcaat gcacgcaacg gctggctgtt tacccgtttc
    11761 ggcgatttga aaatcaaaaa cgccaaacac aagctcgaca agcgtccgat agacgaaagc
    11821 tgcacctgct acgcctgcca aaacttcagc cgcgcctacc tgcaccatct gcaccgcacc
    11881 ggcgaaatct tgggcgcaca gttgaacacc atccacaatc tgcatttcta ccaagtcatt
    11941 atggcggaaa tgcgcgaagc cgtcgaacaa ggcaaatttg ccgactggca ggcgcgattc
    12001 cacgaaaacc gcgcacgcgg cacagactga ttccctgcaa tgccgtctga agccgcttca
    12061 gacggcattt tgccgcttta ccctacccgc actatttttc agaaaccgat catgcttcca
    12121 ttctttccag aaccctccct ttcctatacg caacaaaacc gtaccgccgt tttgctgttg
    12181 aacctcggca cgcccgacgc gccgaccgcg caggcggtac gaccttatct gaagtccttt
    12241 ctgaccgacc ggcgcgtcgt cgaactgccc aaatggctgt ggtatcccat tctgcatggt
    12301 ctggtactga cgctccgccc caaaaaaagc gcgcacgcct atgaaaaaat ctggtttaag
    12361 gaaggttcgc ccttagaagt ttacacagca cgccaagctg ccgcgcttgc caaacgtatg
    12421 cccgacctca tcgtccgcca cgccatgacc tacggcaatc cttcggtggc agacgtattg
    12481 tcggaactga aggcgcaagg cgcgggcaga ctgctggtca tcccgatgta cccgcaatat
    12541 gccgcttcca gcagcggagc ggcagtagat aaggtgtgcg aacaattact gctgcaacgg
    12601 aaccaaatga gcgtccgcac cgtttcgcgc ttttatgacg ataccggcta cattgatgcg
    12661 atgaaaaacc acatcctccg atattgggcg gaacacgggc gcgggaaaaa actgatgttg
    12721 agttttcacg gcgtaccgca gaagcaccac gacctcggcg acccctatcc cgacgagtgc
    12781 cgccacaccg ccaaactgct tgccgaagca ctggaactga ccgaagacca atatgtcgta
    12841 tcgttccaaa gccaattcgg cagggcaaaa tgggttacac cgagcacgca ggatttgttc
    12901 ggcaaactgc ccaaacaggg ggtaaccgag ctggacgtat tttgcccggg ctttttggca
    12961 gactgtttgg aaaccatgga agaaatcgcc ctgatggggc gggaacagtt ttacgaagca
    13021 ggcggcaaaa gctaccgcta catcccctgc ctcaacgaca accccgactg gatagacgca
    13081 ctcgtcgcac ttgccgaaga aaaccttggc ggctggcgtt gaaactgttt gacccatgtc
    13141 taaaatgccg tctgaacgcc gtgttcagac ggcattttcc cgctacttta gcgtatgatg
    13201 gcatctatgc cggacaacaa catcaagtgc aaccttacta ccgattcggc aaaaaaatct
    13261 ttcacccgct acgcaaaatc aacaacaaag tttcaaacat taaggaaaca ccaatgaaca
    13321 caacccgact gccgaccgcc ctcgtcttgg gctgcctctg cgccgccgct tctgccgccg
    13381 acaacagcat catgacaaaa gggcaaaaag tgtacgaatc caactgcgtc gcctgccacg
    13441 gcaaaaaggg cgaaggccgc ggaaccatgt ttccgccgct ctaccgctcc gacttcatca
    13501 tgaaaaaacc gcaggtgctg ctgcacagca tggtcaaagg catcaacggt acaatcaaag
    13561 tcaacggcaa aacctacaac ggattcatgc ccgcaaccgc catcagcgat gcggacattg
    13621 ccgccgtcgc cacttatatc atgaacgcct ttgacaacgg cggcggaagc gttaccgaaa
    13681 aagacgtaaa acaggcaaaa ggcaaaaaaa actaaacaga caaaatgccg tctgaaaccg
    13741 gcaatccggc ttcagacggc attcaaatca aacctttcta acgcggtact ttcagcccca
    13801 tagacccgat attgccgtcc ccgtcaacct ccttcgcggt cagcataaag ccgccgatgt
    13861 caatacggcc gcactcgacc ggctcctgac tgtcggaacg cttatcgaaa agctcggcaa
    13921 ggctcaaacc ctcttcgccc gcttccagcc tcaaaccgta agcaagtgcc aaatcacccg
    13981 aacgtgctgc cggcaaaacg acaaactcac cgaagaaatc aaaatactca cggacgctaa
    14041 tacccgtctc ggtaaagtat ttcgccattt tatagtgaa
//
LOCUS       DAWKST010000041        13952 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.041, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000041 DAWKST010000000
VERSION     DAWKST010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13952
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.041"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(197..4366)
                     /locus_tag="WJL87_000850"
     CDS             complement(197..4366)
                     /locus_tag="WJL87_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ7799300.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRINPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTLWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDRKTGISAKGGARGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNINRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGTLKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNGKGK"
     gene            complement(4421..6268)
                     /locus_tag="WJL87_000851"
     CDS             complement(4421..6268)
                     /locus_tag="WJL87_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ7799301.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKGGAYTVHITPGPRTKITNVGVTILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKAYPLAKLGNTRAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDVDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLTGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMELKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6680..7909
                     /gene="sstT"
                     /locus_tag="WJL87_000852"
     CDS             6680..7909
                     /gene="sstT"
                     /locus_tag="WJL87_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ7799302.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIILYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALAVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8021..9304
                     /locus_tag="WJL87_000853"
     CDS             8021..9304
                     /locus_tag="WJL87_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ7799303.1"
                     /translation="MFERSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPVVAE
                     KETEVKEDAPQAGSQGQGAPSTQGSQDMAAVSAENTGNGGAATTDKPKNEDEGPQNDM
                     PQNSAESANQTGNNQPADSSDSAPASNPAPANGGSNFGRVDLANGVLIDGPSQNITLT
                     HCKGDSCNGDNLLDEEAPSKSEFENLNESERIEKYKKDGKSDKFTNLVATAVQANGTN
                     KYVIIYKDKSASSSSARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGN
                     IFAPEGNYRYLTYGAEKLPGGSYALRVQGEPAKGEMLAGTAVYNGEVLHFHTENGRPY
                     PTRGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGGGDVSGR
                     FYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(9380..9595)
                     /locus_tag="WJL87_000854"
     CDS             complement(9380..9595)
                     /locus_tag="WJL87_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799304.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVFGQYFSEKKMKDDGFIN"
     gene            complement(9588..9803)
                     /locus_tag="WJL87_000855"
     CDS             complement(9588..9803)
                     /locus_tag="WJL87_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEZ7799305.1"
                     /translation="MKEYKVVIYQESQLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(9878..11221)
                     /gene="argG"
                     /locus_tag="WJL87_000856"
     CDS             complement(9878..11221)
                     /gene="argG"
                     /locus_tag="WJL87_000856"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ7799306.1"
                     /translation="MSQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEVSPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEGAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKK"
     gene            11346..12146
                     /locus_tag="WJL87_000857"
     CDS             11346..12146
                     /locus_tag="WJL87_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ7799307.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDHTRQAAAAALELPVVRHPEAAGFIEG
                     ITQKRGEPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLDTYYAGRFNQTQRGSRSVYVFEQPESRITPIMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            12186..13172
                     /locus_tag="WJL87_000858"
     CDS             12186..13172
                     /locus_tag="WJL87_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ7799308.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDMMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFQKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPDIPVYLVAEDMCASG
                     CYYIAAAADKIYADSSSIVGSIGVIGSSFDATELMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKAGVREALQAVR"
     gene            13830..13952
                     /locus_tag="WJL87_000859"
                     /pseudo
     CDS             13830..13952
                     /locus_tag="WJL87_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         2931 a         3569 c         4190 g         3262 t
ORIGIN      
        1 gtacggcgtt gccttgcctt agctcaaaga gaatcggttc cgtactattt gtactgtctg
       61 cggcttcgtc gccttatcct gatttttgtt aattcactat atcgattctg ttcccttgca
      121 ggcggacggc ggcggcggac ggtggaaaag caggttttca aatgtccggt ttggcggcgc
      181 gccgtctgaa aaccgcttat tttcctttgc cgtttccggc ggagtctttt ttgtccgaac
      241 cggagaagcg gtcgaaacgt atggtgtatg tcagctcgcc gcccgacgaa cggctaccga
      301 tacgggcaac cgcctgtatg gcgcgtgtca gccggtaaat cagtttgacg gactgttccg
      361 cgctggagat gctgtattcg tagccgatgt agagtttgcc ggtcagttgt ttgccgacgg
      421 tcagcacctg ttcggcgggg ttgagttcgc cggtttgcgc gttgcggctg cgcttgctgg
      481 taaagcccaa atcatccacc agcccgatgc ggtcgttgat ttgcccggca agcagcgcgc
      541 ctgcggctgc ggacagggcg gcattgtcgc cgctgctgcc actgccggcg cggttgagga
      601 tgagccagga gagcttgtct ttttcactca tcggttcgtt tgccgtcagc gtaatgcgcg
      661 gactgttgag gctgcccaat atttccacgc ccgcaccgac gggggaaagg cggcgttcgg
      721 cgcggatgtt gaggttgggg tcgttgagcg ggccgacaaa ggagaccgtg cctttggtaa
      781 tgtccaaatc ctgcccgtat gccttataac gccctttgat gacgcggacc gtgcccacgc
      841 cccgcacgct tccgcccgat tgggcggtca gggtcagttt gccgcctatg gtaacgtccg
      901 cgccgtagcc ggcgaagcgg atgccgtcat tgaggtctaa agtcaggttc atattgacgg
      961 ggagcggtgc cgccgcctct tttttaactt cgcctaatac gacgacatcg tcgccgacgg
     1021 acggcatcga ggatttttgc gaaccgaaca gcccctgatc cgttttaatc atcccggtaa
     1081 cggatatgcc tttttgcggc gaatagcgca ggcgggtgtt gccggaaacc gtcaggcggc
     1141 ggttagggcg ggacaggatg cggtatttgt cgaacaccgc gccgatatcg acatcgggtc
     1201 cgctgttttc cataccgacc gtgccggaga gttccgccgt tccttcgtgc cggaatttca
     1261 ggctgtcgat tacccatttt ctgcccgcga tatgcgaacg cagcgagccg ttgtccaaga
     1321 tgatgccttg ggtttggttg cggtaataga gcttgtcgcc gttgatgctg ccgcccaagt
     1381 gcgggtcggc gatgctgccg ccgagggtta cggcggcatt caggctgcct ttgacggttt
     1441 gtccgacggg caggaagttg cggaatactt cggcatcggc aacggtcagg ttgagcctgc
     1501 cgcccaaagg cgcggtatcg aaagagcggc tttgcccgac ggtgatgttg ccgttgattt
     1561 tcccgtagtt gctgctgccg ttgacggcgg cattgacgga cggagagccg acccgtccgc
     1621 cgatttgcgc ggcggcattc aggctgccgg taatgttttg cgcggcggcg ggcagaaagg
     1681 gcttcaatgt gcccaagtcg ggaagggagg cggtaatcct gccgccgagc ggtgcatttg
     1741 ccatattgcc gccgaaggcg ttgccgatgt ccaaatcggc gttaatccgc ccgaaacgtg
     1801 cgccgccgtc aagcaggatt ccgatacggt cgttttgaaa gcgcgttttc agggaaaatg
     1861 cgttcaaacc caaagcctgc ccgccgggca atacggcatc gccgctttgc cgattgatat
     1921 tgaggtagcc gcgcgcgttg cgcccgtagg cgacatccca gtcgccgttt aaaaccagat
     1981 tgtgttcgaa gggcggtttg aagaaattgt gcaactcggc gatatgcaga cctcgtgcgc
     2041 cgccttttgc cgatatgccg gttttcctgt cccaggaaaa gtgttgcagg ttgaggctgc
     2101 cgcccatagc ctgccaattt gccgcacttg ccgccacacg ttccgcaccg gcttcgagcg
     2161 tcatacggtt ttgcagtttt aggttgaatg cgccgccgat gtcgagaatg ccgatgctgc
     2221 ccttccatag ggtaagttcc ctgttgatgc cgcctgaagc gtccaaatcg aatttgaacg
     2281 gtttgccatc cagcgtcatg gcggcgtgtg tgcggatgcg gtgctgcacg cccgtgccgt
     2341 ccagcatcag gtcggcggta tcgacaaccg ccgctccgcc cgacagcgaa aggcggctgc
     2401 ctttgatgtc ggcgcgtatc gggcggcttg tgtcgggcga acctttgagc gtgaaatcga
     2461 gcgaacggat gtctgccgcc ttgccgatgt gcaggttgcg cgccgcgccg gaaaggtcgg
     2521 tttcaaaggt tcggatgccg ccgtccaaat cgccggaaag gtgtccgcgt acatttaaag
     2581 accccgcgag tccgaaaccg aaacgggata aatcgggtgc ggtgatgttg aggttgagcc
     2641 ggtcgccttt tttgccgaag ccgccgtctg ttttaataat gttccgcccc agccgcaaat
     2701 cgacggcggc acgcggaagg tggcgggact cgtaaacaat gtcggcactg ccggcaatcg
     2761 gtacgccgtt gaacgtgccg ggtaaaaacc gcattttgcc tgtgaatttc tcttttgcca
     2821 gttcgccggc aaggtttatt gagccgttga tattgcctgc cggaagttgc ggattgatgc
     2881 gcgaagggtc gaatgcgcgg gaacggatgt ccagcttgag caggcggtct ttaaacagct
     2941 cgagatagcc ttgcgcggtc aggctgcctt gcccggcggc gatgttgacg gtgtcgagca
     3001 ccagtttccg ctgtccgttt gcggggtcgc ttgcaatggc gaggctgccg tccgtgcgcg
     3061 ccgtgccgat gccgagttgc caagagattt tgggcgaggc ggtcgtgcca ccgatgccga
     3121 tgctgccgtc caacctgcct ttgaacgcgg tttgcagtac gtcttccgcg ccgacggagt
     3181 tgatgcctat atttaaatcg aggatgtctt tttcggtgtc gattttgccc gacagcctga
     3241 tgccgccccg tccgagcagg gcggcggacg tattgccgat atgcaccgtg ccgtcctgcc
     3301 ggatgacaaa gccgcctaaa acctgacgga cggggatgcc gttgcggtcg gcaaagccgg
     3361 ctttggtgtt ttccaaatcg agcgaacctt ccagcgcgat gccgtctgaa aacgacggga
     3421 tggcggtcag gtcgaaattc agcccggcat cgggcaggga gggcacgaag gcggccgggt
     3481 tgatgttgaa ccctttgacc agcacttctt ccaatgtttt atccaatgat tcggcaaacg
     3541 ggtggatgac ggattttccc gagaggcgga tattgccgcc gtcgatcgcc agttcggcgc
     3601 gcacatcctt caggctgccg ctcagccgcg ccgtactgtg tatggttttg ccttcgagtc
     3661 cgcctttggt gtaaatggcg gtatcgaggg caaacggttt tttcaagccg accgaggctg
     3721 accccgacga actgctccac ggcgtgtcgg cggccttcag gtcgaggcgg tgtcctttgc
     3781 ggtcgtaacg gtatgaagca tccagccgtt cgagatagac ggtttgtttg tcaaaggctt
     3841 tgcccatgct gattttgccc gtctcgaagc ggtcgagata gacggcggca ggcaggtcta
     3901 tgctgtcggg aaggctgagc ggagggcgtt cttctttagg cggagtcggt ttggtaacga
     3961 tggcgatgtc gccggcggaa atttcggtaa tgtgcaggct gcggcgcatc agttcggacg
     4021 gtttccacgc gaagcggaag cggctgattt taaggtctgc cccctcggtt tctatcgacc
     4081 agttgtcgcc gtcgaagccg tcgagcagcg tgcctttgag gttttgggag gaaatgttta
     4141 cgccgaacca agacgggatt tggtacagcc cgaagcgcaa acccgcttcc gtgccggcga
     4201 gccagccgag gaaacatact gccaaaatca ggacagacag cagtgccgcc gacagcttca
     4261 gcagcgggcg gcgttttttt gccggcgggg tggggcggtg ttcagacggc attttgcgcg
     4321 tgccgttttc ggtcggatcg gtatctgtcg gtgcggtatc ggtcataatg tctgtttcaa
     4381 aatagtttgc tgcaatgccg tctgaagcgg ccgtatcggt ttagaagcgt gttcccaagc
     4441 tgatgtgcca gcggattttc ttatcgctgt gcccgtaggc gatgtcgaag gaaaacggcg
     4501 cgagcgggct gaaccagcgc acgcccagtc ccgaaccgtg tttcagctcc atacgtttga
     4561 aattggcggc ggcatcgccc atatcgtgga ataccgcgcc ggaaagggtg cgcgtaaacg
     4621 gcagttggta ttccaggctg cccgtcaaga gggcgcgttc gggcaggacc gatccgttcg
     4681 ggccggcaag cccgatgctg tcgagttcgt aaccgcgcac ggaagacgcg ccgccgctgc
     4741 ggaacatcaa ccccgaagga acgtcggcat tgtcgcgggc aacggtgtaa cctgcttgtc
     4801 cgcgtatgat gaacgtgccg agttttttgt tttcgggcgt gaagaaataa cctgcgcggg
     4861 cagaggtgcg gatcagcgcg gtggaggaca ggaatgtgcc caaagtcgta ccgattttgc
     4921 cgtcgaggta atggccgttt tcgggatgca gcacgttgtt gagcagctgg cgtttccaag
     4981 aggcggtcag catcgtggcg tggctgttgc ccaaatcgac atccgagccg gggattttcc
     5041 ggccttctgc gagaaactcc gcccccagcc tggcatcgat gcccgcgcgg tcgcgcacat
     5101 accagatgcc gccggagaag gcgcgttttt cgaggttttg ggtggtcgaa cggttgtagg
     5161 agacgttgct tgtccagtag ttgccccgat agttgcgcgg ctggctgatg ccggcggcaa
     5221 gcgtggtttc gtatttgtcc atatcccaga cgaccgaacc gatatagcct ttgttgaaga
     5281 ggttgtaata gtcgtaggcg attttgccgc ccaaaccgta ttccgaatcg aggcggatgc
     5341 cggtttcgag tttgtggcgt ttgacctcgg ttacgctgac tttgacgggg acgcggtcgc
     5401 cttggaggcg gtcgaagtcg gcttgtacgg acgcgccgga ataatgcccg ttttgttcga
     5461 gcgcctgttg gaagtcgagc agcaggtcga ggtcgtaggg cgtgcccggt tggaagcgcg
     5521 ccaggccgga gacgatttgt tcggggtaac gctgtgtgcc ggtgatttca aagtcgccga
     5581 aggcgatggg gcggccgctg tccacgacga cgttcaaatc ggcggtggcg gtatcggggt
     5641 tgacggccgc ccgggtgttg ccgagcttgg caagcgggta ggctttgcgc gttaccgcgc
     5701 cgaggacgga agttttgctg ttttcccaac tgtcctgatc gaaatcgctg cctaccggct
     5761 gctgccagtt ttccagcgcg ttgcggtagt attcggcgag gttgccgtct gaaaggatgt
     5821 cgccgaggat ggtgacgccg acgttggtga ttttggtgcg cggccccggt gtgatgtgca
     5881 ccgtataagc cccgcctttt tccgtcaggc tgactttgct gctgaaataa cctttgctgc
     5941 ggagcatcgt tttaacgttg tccggtgctt cttcggcgag gaagcctgtc tgttccttgt
     6001 ccaatacttc ttcctgctgc tgcgtgatga gcggcaggtg ttcttcgacc atatctttga
     6061 tttcactgtc ctgcgtgtcg atgcggacgg ggaatttggg ttttaatttg actgattcgg
     6121 tgtcggggct tttgtttttg aacaatgcga aacccgccgc cttgttttcg gaaaggtcgg
     6181 cggcaggcgc gtatgcgtgc ggaaagaaaa ataaagccgg caggagcagg gcggtcggtt
     6241 tgatcatcat ggtacgggtg tcgtgcatcg gtgtggcaga tttcgattct aacactgttc
     6301 ggacagggtt tcgaacggct gtttttgtgt tgttcggacg gggcgggaca cttcagacgg
     6361 catactgccg ccggtatttc ggaggttgcg gcccgccgga aatgtatctg cttgttttaa
     6421 ggtattttgt ttccaatccg catatggaaa acaggctgcg tttcggaata gtgccggaaa
     6481 ataggcagga ttaaataaat ttattttcgt tcaataaatt aaaccgttgc gccgaatgat
     6541 aaagcaaagc ctgtccggca ctgcataggg caaatcaacg caaccttaaa acacagccga
     6601 aaaataaaaa aaaacacaga gaatgagttt tcttttaaaa tacaacattt tttaacatat
     6661 cacaacataa ggaagaatta tggcgttcgg caaatcctta tttcatgcga tcggccgcgt
     6721 cagcctggtc aggcagattg ccgccggttt ggcgttgggc atcgtaatcg gttcggtttc
     6781 cccgcaactg ggcttggcgg caggcttgtt cggcagcctg tttgtcggcg cgctcaaagc
     6841 ggtcgcgccg gttttggtat ttattttggt ggcggccaca atcgcgcagc accaaaaagg
     6901 caacaaggcg catatcaggc cgattatcat cctttacctc atcggtacgt tttccgcagc
     6961 cctgaccgcc gtcatcgccg gtatggtttt cccgacgcac attgttttgg cgggcgcggg
     7021 cgatgtgtcc gccgcgccgc cttccggcat tgtggaagta ttgaaatcgc tgctgatgaa
     7081 cctggtcgcc aacccgatta acgcgattgc caatgccaac tatatcggca ttttggcttg
     7141 ggctttggtt ttgggcgcgg cgttgcggaa tcacggttcg gacgttacgc ggcaggtcgt
     7201 tgccgatttg gcggaagcgg tttccaccgt cgtgaaatgg attatccgtt ttgccccttt
     7261 gggcattttc gggctggtgt cgtccacaat cgcggagacg ggtttcggcg cgctggcggg
     7321 gtacgcgaag ctgcttgcag tgctgttggg ctgtatggcg tttatcgcgc tggcggtcaa
     7381 tcccgccatc gtgtggtgga aaatccgccg caacccttat ccgctggtgt tcacctgcct
     7441 gcgcgaaagc ggcgtgtatg ccttctttac ccgttcttcc gccgccaata tccccgtgaa
     7501 tatggctttg gcgaaaaaac tggggctgca cgaagacact tattcgattt ccatcccgtt
     7561 gggggcgacc gtcaatatgg gcggcgcggc gattacgatt accgttttgg ctatggcggc
     7621 ggcgcacacc caaggcatac aggttgattt tgccaccgcg ctgctgttga gcctggtggc
     7681 gacggtcagc gcgtgcggcg cgtccggcgt ggcgggcggt tcgctgctgc tgattccgct
     7741 ggcgtgcagc ctgttcggca tcagcaacga tgtcgccatg caggtggttg ccgtcggctt
     7801 tatcatcggc gtgattcagg attcggcgga aacggcgttg aattcttcaa ccgatgtttt
     7861 gtttaccgct gccgccgatt tgggccgaca gagaaaccgg gcggaataaa ccaagctatg
     7921 ccgtctgaag cccgtttggc gttcagacgg catattttta caattttatg ggcatatggc
     7981 ggaaacaata ccggctgcct tgaatatgaa ggggatgatg atgtttgaac gcagtgtgat
     8041 tgcaatggct tgtatttttg ccctttcagc ctgtgggggc ggcggtggcg gatcgcccga
     8101 tgttaaatcg gcggacacgc tgtcaaaacc ggccgctcct gttgttgctg aaaaagagac
     8161 agaggtaaaa gaagatgcgc cacaggcagg ttctcaagga cagggcgcgc catccacaca
     8221 aggcagccaa gatatggcgg cagtttcggc agaaaataca ggcaatggcg gtgcggcaac
     8281 aacggacaaa cccaaaaatg aagacgaggg accgcaaaat gatatgccgc aaaattccgc
     8341 cgaatccgca aatcaaacag ggaacaacca acccgccgat tcttcagatt ccgcccccgc
     8401 gtcaaaccct gcacctgcga atggcggtag caattttgga agggttgatt tggctaatgg
     8461 cgttttgatt gatgggccgt cgcaaaatat aacgttgacc cactgtaaag gcgattcttg
     8521 taatggtgat aatttattgg atgaagaagc accgtcaaaa tcagaatttg aaaatttaaa
     8581 tgagtctgaa cgaattgaga aatataagaa agatgggaaa agcgataaat ttactaattt
     8641 ggttgcgaca gcagttcaag ctaatggaac taacaaatat gtcatcattt ataaagacaa
     8701 gtccgcttca tcttcatctg cgcgattcag gcgttctgca cggtcgagga ggtcgcttcc
     8761 tgccgagatg ccgctaatcc ccgtcaatca ggcggatacg ctgattgtcg atggggaagc
     8821 ggtcagcctg acggggcatt ccggcaatat cttcgcgccc gaagggaatt accggtatct
     8881 gacttacggg gcggaaaaat tgcccggcgg atcgtatgcc ctccgtgtgc aaggcgaacc
     8941 ggcaaaaggc gaaatgcttg ctggcacggc cgtgtacaac ggcgaagtgc tgcattttca
     9001 tacggaaaac ggccgtccgt acccgactag aggcaggttt gccgcaaaag tcgatttcgg
     9061 cagcaaatct gtggacggca ttatcgacag cggcgatgat ttgcatatgg gtacgcaaaa
     9121 attcaaagcc gccatcgatg gaaacggctt taaggggact tggacggaaa atggcggcgg
     9181 ggatgtttcc ggaaggtttt acggcccggc cggcgaggaa gtggcgggaa aatacagcta
     9241 tcgcccgaca gatgcggaaa agggcggatt cggcgtgttt gccggcaaaa aagagcagga
     9301 ttgatgctga tgctgtctga agcgggggcg ttcagacggc attgtgatgt ttgggttgcg
     9361 ggtccggttc aagtccgcat cagttgataa acccgtcgtc tttcattttc ttttcggaaa
     9421 aatactggcc gaataccagc aaggcggcga ggatgatgac aaacagcccc gccatagtca
     9481 gtgtgatttt gaagaacaat tccgcttcga gaaaagaaaa ccaaagttgc acaatcagca
     9541 gcagcaccca gccggcaaac agtccgagac aggcataaac gccaagctta aacacggtcc
     9601 cgctccaaaa tgacgacgta ggcctcgcgt ttgaaaaaca acagcatacg gcgcaaatct
     9661 ttttccatcg ttacaacccg ccagccttcg ggggtttgtt tgttgaggaa cgcgctgaaa
     9721 ttgacggggt tgacctttgc cgcgccgaaa aacaggctgg acaactggct ttcctgataa
     9781 atgacgactt tgtattcttt cataggattc cttttaacat aagggatcat cttttcagac
     9841 gaccccttaa gttgctaatg atgcaaaaca gcaaacctta tttcttattg cccaactgcg
     9901 gtaataccga gccttcgccc agcgacagca aaccgctttg cgagtagatg cccagtttgg
     9961 cgcgggtgtc ggtgatgtcg aggttgcgca tcgtgagctg tccgatgcgg tcgagcgggg
    10021 taaacgccgc accttcgact ttttccatac tcaggcgttc gggttggtag gtcaggttgg
    10081 gcgattcggt gttcagaatc gagtagtcgt tgccgcgccg cagttcgagg gtaacttcgc
    10141 cggtaacggc tttggcgacc cagcgttggg cggtttcgcg caacatcaag gcttggctgt
    10201 cgaaccagcg gccttggtag agcaaacggc cgaggcgcag gccgttgatg cggtattgtt
    10261 cgatggtgtc ttcgttgtgg atgccggtca ccaagcgttc gtaggcgatg tggaacaacg
    10321 ccatacccgg ggcttcgtag atgccgcgcg atttggcttc gatgatgcgg ttttcgattt
    10381 ggtcgctcat acccaagccg tggcggccgc cgatgcggtt ggcttcgagg aagagttcga
    10441 cggggtcggc gtattctttg ccgttcagtg caaccggcac gccttcttca aagcgcacgc
    10501 tgacttcttc ggggctgact tcaacgtttt cgtcccaaaa ggcaacgccc ataatgggtt
    10561 tgacgatttt gatgcccgag ttcaaaaatt ccaagtcttt ggcttcgtgg gtcgcgccga
    10621 gcatattgga atcggtggag taggcttttt caaccgacat tttgtagttg aagccgttgg
    10681 caatcagaaa ttcgctcatt tcgtgacggc cgccgagttc gtcgataaat tgctggtcga
    10741 gccagggttt gtagattttc agcgcgggat tggtgagcaa accgtagcgg tagaaacgct
    10801 cgatgtcgtt gcctttgtag gtgctgccgt cgccccagat gtttacgtcg tcttctttca
    10861 ttgcggaaac aagcatcgtg ccggttacgg cgcggcccag aggcgtggtg ttgaaatagg
    10921 cgatgccgcc ggtggaaacg tggaacgcgc cgcattggat ggcggcgatg ccttcgtgtg
    10981 ccaactgcgc gcggcagtcg attaagcggg cgttttccgc accgtattcc atcgcttttt
    11041 tgggaatggc gttgtagtcg tcttcatcgg gctgaccgag gttggcagtg taggcataag
    11101 gcagtgcgcc tttgagtttc atccacaaca gcgcggcgga ggtatcaagt ccgccggaga
    11161 aggcgatgcc gactttctga ccgacgggga ggttttgtaa aatagtatgg ttttggctca
    11221 tgaaaaaatc ctcggtagct tgaaaaatca aagccattat cttataaata ggaaatattg
    11281 tcagccatca tgccgtctga acgggtattc cgccgtttca ggcggtattt aaaaccgaaa
    11341 acattatgaa tgcgttcaac ctcatcatca tcggcgacga aatcttacac ggcagccgcc
    11401 aagacaagca tttcgccttt ttcaaatccc tgctcgaatc caaagggctg aagctcaatc
    11461 aggtgcaata tctgcccgac gaacccgatt tgctggtcag gcaactgcgc cgcagctttt
    11521 cagacggcat accgacgttt gttaccggcg gcatcggcgc cacgcccgac gatcataccc
    11581 gccaagcggc cgccgccgct ttggagttgc ccgtcgtccg ccatcctgaa gccgccgggt
    11641 ttatcgaagg cataacccag aaacgcggcg agccgcttga ttcgccggaa cacgcccaac
    11701 gcctgaaaat ggcagatttc cccgaaggcg cggaattggt gcccaatccg tttaataata
    11761 tcgccggatt ttccattcgc gagcattatt tcttccctgg ttttcctgtg atggcgcacc
    11821 cgatggcgga atgggttttg gatacttatt acgccggccg cttcaaccaa acccaacgcg
    11881 gcagccgcag cgtgtatgtg ttcgagcagc ccgaatcgcg cattacgccg attatggagc
    11941 atatcgagca aacttatccc ggcgtgcgtt cgtacagcct gcccagcgtc ggttggacgc
    12001 attcagacgg cacgcaggtc aaaccgcaca ttgagttcgg catcaaagcc gaaggagaag
    12061 ccgtcaacct tttggacgcg gcgtgggcgg aagtgttgca cagcttggac ggattgggag
    12121 cagagctgaa aaatcgggta aactgaaaac cgtttgccca aatcggacga tatttaagga
    12181 aaaccatgca ataccgaatc agaagagaaa acgaagcacc cgaagcaaaa aatgcggggg
    12241 aaaccttatg ggagcgcgat atgatgcgcg aagtgctgtt gtccgcctat caggacaggc
    12301 gcagggagcg gatgtggaaa aacatctggc gcgccgtcag caccctgatt ctggttgccc
    12361 tgattgcagg cattttccaa aaagacgaag ccgcattgca gttggcgggc aacacgccgc
    12421 ataccgcagt cgtcaacctg tacggcgaaa tcggcaacgg tgtagaagac caggtcaaaa
    12481 aactcaaaga cggtatggag gccgcctaca aaaacccgca ggcaaaagcc atcgtcatcc
    12541 gcgccaacag ccccggcggt tcgcccgtcg tctccaacac cgcttttgaa gaaatacgcc
    12601 gtctgaaagc gcaacatccc gacattcccg tttatctcgt ggcggaggat atgtgtgcat
    12661 ccggctgcta ctatattgcg gcggcggcag acaaaattta tgcggactcg tccagcatcg
    12721 tcggcagcat cggcgttatc ggcagcagtt ttgatgctac tgaattaatg gaaaaaatcg
    12781 gcgtgaaacg cagggttaaa attgcaggca gcaacaaagg catgggcgat ccgttttcgc
    12841 ccgaaacgcc cgaacagtcg aaaatctggg aagaaatgct gaccggcata cacggcgagt
    12901 tcatcaaagc cgtcaaaacc ggacgcggcg gccgcctcaa attccgacaa tatcccgacg
    12961 tgttcagcgg tcgcgtgtac acaggcgcgg atgcgctgaa agtcgggctg gtggacggac
    13021 tcggcaatat ttacagcgtg gcgcgagacg tggtcaaagc ccccgatgtg gtggattaca
    13081 ctccgaagga cgatttcggc agaatcttgg gcagacgctt cggcgcagaa ctcaaagccg
    13141 gcgtgcgcga agccctgcag gccgtccggt agcgttgttt acagtatttc taagggaata
    13201 ccaaaataaa aatatacaca aaaacactca cctccccagt atgctgccac taagtcagcc
    13261 gcgtcactat ttaccgtgca cttaaagccg taaaaggcag gttgtccccg ggaggttttg
    13321 tgcatcctga taccgatcgg ttgttgttgc tcaaaggaca gaaggccgct gataaacgag
    13381 attacctgtt tgtcgctatt gacgattttt atactctgcc actttgccgg acaaaaccgc
    13441 agacagtgct gccaagtttc tgaccgaaca tctggccgac ccctgcttgt acctgattga
    13501 gtacgtttac tctgacaatg ataggtaata taaagagccg tccaacatgc tttcggtgca
    13561 gcttgttatg ataatgggat tggttggagg cttgcccgat ttgcttgtcc gcagaccaac
    13621 ggtaaggcgg agcgggttat ccgtaccttg atggagatgt ggcatgagga acagtcgttt
    13681 gacagtccgg aacatcggca aaaggagttg tgctgcttta ttaatttagg ggctgcccta
    13741 gatcactagg gaaattcaaa ttaagttaga attatcccta tgagagaaag tcgtctaagc
    13801 cggtaataaa caaaataaac tcattttgtt atcgtggcta tgatgtattg gatgcgggcg
    13861 aatttagcca tttccgtatc aatcacagca cacattttgc cgaacgacaa aaccatatta
    13921 atggaactga gaacttttgg aatcaagcaa aa
//
LOCUS       DAWKST010000042        13819 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.042, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000042 DAWKST010000000
VERSION     DAWKST010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13819
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.042"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(122..1447)
                     /locus_tag="WJL87_000860"
     CDS             complement(122..1447)
                     /locus_tag="WJL87_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ7799309.1"
                     /translation="MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPAPAGTTVAGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNTGGTHTADLSQFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGKYMADKGYLKLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFF
                     RELTGSGNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            1659..2498
                     /locus_tag="WJL87_000861"
     CDS             1659..2498
                     /locus_tag="WJL87_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HEZ7799310.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGLGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKRQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIAVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVITWFLCGSRPSETLTRKE
                     ELK"
     gene            2495..3661
                     /locus_tag="WJL87_000862"
     CDS             2495..3661
                     /locus_tag="WJL87_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ7799311.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGA
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            3925..5190
                     /gene="efeB"
                     /locus_tag="WJL87_000863"
     CDS             3925..5190
                     /gene="efeB"
                     /locus_tag="WJL87_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEZ7799312.1"
                     /translation="MSKKQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(5262..5591)
                     /locus_tag="WJL87_000864"
     CDS             complement(5262..5591)
                     /locus_tag="WJL87_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEZ7799313.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVL
                     DANGAALQNGDTVILIKDLKVKGSSMVIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(5668..7038)
                     /gene="glmU"
                     /locus_tag="WJL87_000865"
     CDS             complement(5668..7038)
                     /gene="glmU"
                     /locus_tag="WJL87_000865"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ7799314.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHRIGGKPMLGRVIDTAA
                     ALNPQNICVVIGHGKDQVLNAVKRDVVWVEQTEQLGTGHAVKTALPHLSAEGRTLVLY
                     GDVPLIDVKTLKTLLEAAGNEVGLLTDVPADPTGLGRIIRDGNGSVTAIVEEKDADAA
                     QKAVKEINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVHT
                     SYLAAGVNNKLQLAELERIFQTEQAQELLKAGVTLSDPARFDLRGRLKHGQDVVIDVN
                     CIFEGEVEIGDNVEIGANCVIKNAKIGANSKIAPFSHLEDCEVGENNRIGPYARLRPQ
                     AKLAADVHIGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGNEVRIGSNCVLVAPVTLGNKVTTGAGSAITRNVEDGKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            complement(7093..8070)
                     /locus_tag="WJL87_000866"
     CDS             complement(7093..8070)
                     /locus_tag="WJL87_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ7799315.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGIFTLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMPETQNTLSR
                     LKGRKAVFSNGPSFYVRAVVNALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKSHALPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTPYPPKIRKNVMRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTP
                     VYYQDGSYSKNMNYNQYRPERHAVLPDQTDGSEQDVRGQNWQKPKFQNR"
     gene            8129..9130
                     /locus_tag="WJL87_000867"
     CDS             8129..9130
                     /locus_tag="WJL87_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7799316.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPELAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            9224..10072
                     /locus_tag="WJL87_000868"
     CDS             9224..10072
                     /locus_tag="WJL87_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ7799317.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            10099..10584
                     /locus_tag="WJL87_000869"
     CDS             10099..10584
                     /locus_tag="WJL87_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEZ7799318.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(10686..12254)
                     /gene="msrAB"
                     /locus_tag="WJL87_000870"
     CDS             complement(10686..12254)
                     /gene="msrAB"
                     /locus_tag="WJL87_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ7799319.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            12399..13664
                     /gene="ftsY"
                     /locus_tag="WJL87_000871"
     CDS             12399..13664
                     /gene="ftsY"
                     /locus_tag="WJL87_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ7799320.1"
                     /translation="MFSFFRRKKKQETPALEEAQIQETAAKAESETDQVIENIKEEVE
                     SLTESVKEQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAIAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         2969 a         3537 c         4142 g         3171 t
ORIGIN      
        1 aatttgatcc actataattc cgtcaaataa gaaaggaatt ttgtgcctgc ggtatcgcaa
       61 aacttcgcct taatgcgccc gattgcctag ggatgggctt cagacggcat tgttttccgg
      121 tttacgggcg gtattcgggc ttcataccgt tgggcagaag ctgccagaca tatcccgtgg
      181 ttttctgttt gccggcaagt tcgccggctt cgtcgccgta accccaaaaa taatccacgc
      241 gcaccgcgcc tttaatcgcg ctgccggtat cctgcgccat aatcaggcgg ttgagggctt
      301 tgcgggtaac cggatgggcg gtggcgacaa ataagggcgc acccaaggta atgtagtgcc
      361 ggtcgactgc gccggcatat tcccccatca gcggcgtgcc cagtgcgccg acagggccgt
      421 cattgccgct tccggtaagc tcgcggaaaa agatatagct ggggttttga cccaaaactt
      481 cggcgaggcg ttgcggattt tgccgcatat aagacttaat gccctgcatg gaggtttgtc
      541 cgagtttgag gtagccctta tccgccatgt attttccgat ggaaacatac ggatgctcgt
      601 ttttgtcggc atagccgatg cggatgtatt tgccggacgg ggttttcaga cggcccgagc
      661 cttggatgtg cataaaaaaa agttcgacag ggtcttcggc gtaaccgagt atcggtgctt
      721 tgccgtcaag cgcgccgccg ttgatttggt tgcgcgtgtg gtaggggagg aagcggcttc
      781 cttcaaacct gcctttgatt gccgttgtgc gcgcagtgat ggggaattgg gagaggtcgg
      841 cggtatgtgt gccgccggta ttgtcgattg tgccgctgtt ttttcctgtc tgcctgatgc
      901 ggacaagggc ttttccgctc cgcaaaccgg caggcagggg gacggagata aaatcgtcgg
      961 gaataccgta aatcgggaag cgggcttgtg ccgtccgcct gtcgtcgccc ttcagcaccg
     1021 gctcgtaata gccggtaacc gtgccggcaa ggcttccgtt gcctgcaacc tgccacggcg
     1081 tgaaatagcg ttcaaaaaac tgttttgcct gaaaggaatg gacgggggtt tgaaaggctt
     1141 gggcacacac atcctgccag ccttggcggt ttttcaaatt ggcgcagccg aggcggaagg
     1201 attgcaggct tttggcgaaa tcctgcgccg cccagtgggg cagggacagg tgcggcacaa
     1261 cggtataaac ggccccgccg ccggcaaccg tcgttccggc gggggcgggg ataccgaccg
     1321 gccggtccgg gccgttgatg acggatgtgt cgggttgcgg aaaggtttgg atgctcttgc
     1381 tttggcaggc ggcgaggatg gcggcggcga tgccgcacag ggcggcgcgg aacaggtatt
     1441 ttttcataat ggacaatgtt gtcagcagta ataaaaaaag atggtttcgg gcggcgttgc
     1501 agcagccgtg gagaggggat tttaacacag ggcgcagctg cagcctgcgg aactttccgc
     1561 cgcgcggtac tgcagataaa aataacttgc atttgtattt acaagcaatg aaaatattcc
     1621 gataatatat tattcatcat ccttgttcgt tcgcgtttat gctggtcgct tttttaatta
     1681 tgttgcgcga gggtatcgaa gccgcgctca ttgtcggcat cgttgccggt tttctgaaac
     1741 agtccggaca ttccaaactg atgcctaagg tctggttcgg ggttgttctt gcttctttga
     1801 tgtgtttggg gctggggtac ggcatccatt cggcaacagg cgagattccc cagaagcagc
     1861 aggagtttgt cgtcggcatc atcggtttgg ttgccgttgc catgctgact tatatgattt
     1921 tatggatgaa aaaggcggcg cgttcgatga agcggcagct tcaggattct gtgcaggcgg
     1981 ctttgaaccg tggcagcggt caaggatggg ccttggtcgg tatggcgttt cttgccgtgg
     2041 cgcgcgaagg tctggagagc gtttttttcc tgcttgccgt attcaaacag agcccgacgt
     2101 ggcagatgcc ggccggtgcg gtagcggggg ttttggctgc cgccgtgatt ggcgcgttga
     2161 tttatcaggg cgggatgcgc ctgaatctgg cgaagttttt ccgttggacg ggggcgtttt
     2221 tgattgccgt tgccgccggt ctgcttgccg gctcgctgcg cgcgctgcat gaggcaggta
     2281 tttggaacgc gcttcaggac attgtgttcg actcatcaaa atatttgcac gaagacagcc
     2341 cgttgggcgt gctgctcggc ggatttttcg gctataccga ccatccgacg cagggcgagg
     2401 ccttggtttg gctgctgtac cttattcccg tcataacttg gtttttgtgc ggcagcaggc
     2461 cgtctgaaac tttaacccgt aaagaggagc tgaaatgaga aaattcaatt tgaccgcatt
     2521 gtccgtgatg cttgccttag gtttgaccgc gtgccagccg ccggaggcgg agaaagctgc
     2581 gccggcagcg tccggtgagg cgcaaaccgc caacgagggc ggtgcggtca gtatcgccgt
     2641 caacgacaat gcctgcgaac cgatggaact gaccgtgccg agcggacagg ttgtgttcaa
     2701 tattaaaaac aacagcggcc gcaagctcga atgggaaatc ctgaaaggcg tgatggtggt
     2761 ggacgagcgc gaaaacatcg cccccggact ttccgataaa atgaccgtca ccctgttgcc
     2821 gggcgaatac gaaatgactt gcggtctttt gaccaatccg cgcggcaagc tggtggtaac
     2881 cgacagcggc tttaaagaca ccgccaacga agcggatttg gaaaaactgt cccaaccgct
     2941 cgccgactat aaagcctacg ttcaaggcga ggttaaagag ctggtggcga aaaccaaaac
     3001 ctttaccgaa gccgtcaaag caggcgacat tgaaaaggcg aaatccctgt ttgccgacac
     3061 ccgcgtccat tacgaacgca tcgaaccgat tgccgagctt ttcagcgaac tcgaccccgt
     3121 catcgatgcg cgtgaagacg acttcaaaga cggcgcgaaa gatgccggat ttaccggctt
     3181 tcaccgtatc gaatacgccc tttgggtgga aaaagacgtg tccggcgtga aggaaattgc
     3241 agcgaaactg atgaccgatg tcgaagccct gcaaaaagaa atcgacgcat tggcgtttcc
     3301 tccgggcaag gtggtcggcg gcgcatccga actgattgaa gaagtggcgg gcagtaaaat
     3361 cagcggcgaa gaagaccggt acagccacac cgatttgagc gatttccaag ccaatgtgga
     3421 cggttcgaaa aaaatcgtcg atttgttccg tccgctgatc gaggctaaaa acaaagcctt
     3481 gttggaaaaa accgatacca acttcaaaca ggtcaacgaa attctggcga aataccggac
     3541 taaagacggt tttgaaacct acgacaagct gggcgaagcc gaccgcaaag cgttacaggc
     3601 ctctattaac gcgcttgccg aagaccttgc ccaacttcgc ggcatactcg gcttgaaata
     3661 agccgcaagc gttcagacgg tatttggcgg cagatgccgt ctgaagtttt atagtggatt
     3721 aacaaaaacc agtacggcat tgcctcgcct tgccttgccg tactatttat actgtctgcg
     3781 gcttcgtcgc cttgtcctga tttttgttaa tccactatat ccgccatata ttgcagggcg
     3841 ggatttcaac ctgccgctat cggttaatgg aaaaacggcg tgcagggata cccatcctgc
     3901 tgcacggata ttgaaggaaa caccatgagc aaaaaacaac ccgcacaacc gaccaggcgc
     3961 actcttttta aaaccgcgat cgcagccgga gcagtcggcg caatcggagg ttatctcggc
     4021 ggcaaaaaac agggcgaaac cgccgaacgc accgccgaaa gccaacactc gccccaagcc
     4081 tatccctgct acggcgaaca tcaggcaggc atcgttacgc cgcagcaggc gttttcgatt
     4141 atgtgcgcct tcgacgtaac cgcgcaaagt gccaagcagc tggaaaacct gttccgcacg
     4201 ctgaccgccc gcatcgagtt tctcacccaa ggcggcgaat accaagacgg cgacgacaaa
     4261 cttccgccag ccggcagcgg cattttgggc aaagccttca accccgacgg gttgaccgtt
     4321 accgtggggg tgggcagcag cctgtttgac ggccggttcg gactcaaaga caaaaaaccg
     4381 attcatttgc aggaaatgcg cgacttctcc aacgataagc tgcaaaaaag ctggtgcgac
     4441 ggcgatttga gcctacaaat ctgtgccttt acccccgaaa cctgccaagc cgccctgcgc
     4501 gacatcatca aacacaccgt ccaaaccgcc gttatccgtt ggagtatcga cgggtggcag
     4561 cccaaatccg aacccggcgc gatggcggcg cgcaacctgt tgggcttcag ggacggcacg
     4621 ggcaacccca aagtttccga ccccaaaacc gccgacgagg ttttgtggac gggtgtggcc
     4681 gccaacagcc tcgacgaacc ggagtgggcg aaaaacggca gctatcaggc agtccgcctt
     4741 atccgccact ttgtcgagtt ttgggacagg acgccgcttc aagagcaaac cgacattttc
     4801 gggcggcgca aatacagcgg tgcgccgatg gacggcaaaa aagaagccga ccaaccggat
     4861 tttgccaaag accccgaggg gaataccacg cccaaagaca gccatatacg cctggcgaat
     4921 ccgcgcgatc ccgaattcct caaaaaacac cgcctcttcc gccgcgccta cagctattcg
     4981 cgcggactcg cctcaagcgg acagcttgat gtcggactgg tgttcgtctg ctatcaggca
     5041 aaccttgccg acggattcat cttcgtgcaa aacctcctca acggcgaacc gctggaagaa
     5101 tacatcagcc ccttcggcgg cggctatttc ttcgtcttgc ccggcgtgga aaaaggcggc
     5161 tttttggggc aagggctgct gggcgtataa atccgccata taaaaaacgc cgtccgaacc
     5221 ttgccaaacg ggttcggacg gcgtttcttg tttttgggcg gtcaggcttt tttgacgaat
     5281 tcggatttta aattcatcgc gctgccgtcg attttgcagc cgatgttgtg atcgccttcc
     5341 tgcaggcgta tgcctttgac ttttgtacct tgtttgatca ccatcgagct gccttttacc
     5401 ttgaggtctt tgatgaggat gacggtatcg ccgttttgca gtgccgcgcc gttggcatcg
     5461 agcacttgag ccgcaaggtc ggcggcggat tcggtttcat tccattcgtg ggcgcattcg
     5521 gggcagatgt attgtccgcc gtcttcatag gtgtattcgg aggcgcattg tgggcatggg
     5581 ggtaatgaca tggtttgcag tccttatctg atatcggagg ggtttgatgc cgtctgaaac
     5641 ctgaaaccgg cttcagacgg catggcttta ttgtttgtct ttttccggcc tcacccagcc
     5701 ttcgatgacg gtttggcggg cgcgggcgag ggcgagtttg ccgtcttcga cgttgcgggt
     5761 aatcgcgctg cctgcgcctg tggttacttt gttgccgagg gtaacggggg caaccagtac
     5821 gcagtttgaa ccgatgcgca cttcgttgcc gatgacggtt ttgtgtttgt gcacgccgtc
     5881 gtagttggca ataatcgtac ctgcgccgaa gttggttttg ctgccgactt cggcatcacc
     5941 aatgtaggtg aggtggttgg ctttggtgcc tttgccgatg gcggcgtttt tgatttcgac
     6001 gaagttgccg atgtgtacgt cggcggctag tttggcttgc ggacgcaaac gggcgtacgg
     6061 gccgattcgg ttgttttcgc cgacttcgca gtcttcgagg tgggagaagg gggcgatttt
     6121 gctgtttgcg ccgattttgg cgtttttgat gacgcagttt gcgccgattt cgacgttgtc
     6181 gccgatttcg acttcgcctt caaagataca gttcacatca attacgacat cttgtccgtg
     6241 tttcagacgg cctcgtaaat cgaaacgtgc cgggtcgctc agggtaacgc ctgctttgag
     6301 caattcttga gcctgttcgg tttggaaaat acgctcgagt tcggcgagct ggagtttgtt
     6361 gttcacgccg gcggcgaggt aggaggtgtg cacttggacg ggatgaactt taataccgtc
     6421 ggcaacggct ttggcgatga ggtcggtcag atagtattcg ccttgggcat tgttgctgga
     6481 gaggctgttc agccagtttt ccagtttggc gttgggcaga accaaaatgc cggtattgat
     6541 ttcttttacg gctttttggg cggcgtcggc gtctttttct tcgacgatgg cggttacgct
     6601 gccgttgccg tcgcggatga tgcggcccaa gcctgtcggg tcggcgggaa cgtcggtcaa
     6661 cagaccgact tcgttgccgg cggcttcgag cagggttttg agggttttaa cgtcaatcag
     6721 gggaacgtcg ccgtacagta ccagcgtgcg gccttcggcg gaaaggtggg gcagggcggt
     6781 tttgacggcg tggccggtac cgagctgttc ggtttgttca acccaaacga catcgcgttt
     6841 gacagcgttt aaaacttggt ctttgccgtg cccgatgacg acgcagatgt tttgcggatt
     6901 cagcgcggcg gcggtgtcga taacgcgccc gagcatgggc ttgccgccga tgcggtgcag
     6961 cactttgggc attttggaat acatgcgcgt gcctttgccg gcggcgagga taacgatgtt
     7021 taaagtgttt tgcggcatga cggtttcctg tgcaatgccg tctgaagcag cttcagacgg
     7081 catagggtag gtttatcggt tttgaaattt gggcttttgc cagttctgtc cgcgtacgtc
     7141 ttgttcggaa ccgtcggttt ggtcgggcag cacggcatgg cgttcggggc ggtattggtt
     7201 gtagttcata tttttcgagt agctgccgtc ttggtaataa acgggcgtgc cggcgggata
     7261 tttttgacgg acggcggtct tgccgttgcc gtcttgataa gtttcccacg cgcagcccga
     7321 caaaagggcg gcggcagtca tcaggataag gaaggtttta cgcataacat tctttcgtat
     7381 tttcgggggg tagggggtat tgtaatgatt ttggcggtgt tctgacaaag tttctgcata
     7441 ccgagccagt tgcgccatat cgcttacgga ggcatcgata aagggcagcg cgtgggattt
     7501 tgcaccgaac cggacggttt tcatacccag cgcctttgcc tgatgcaggt tgtccgcgct
     7561 gtcgtccacc ataatgcagc attcgggcgg tacgtccaac aggcggcaga cattgagata
     7621 cgcttgcgga ttgggtttgt acagcagccc gaaatcatcc gtgccgaaaa gcgcgtcgaa
     7681 acggttttcc aaaccgagtg cgttgacaac ggcacggacg taaaacgacg ggccgttgga
     7741 aaaaaccgcc ttgcgccctt ttaggcggct cagggtgttt tgtgtttcag gcatgccgtg
     7801 cagcctggtc aggattgcat cgatcggatg gctttcgcgc aaaaattcgg cgatgtcgat
     7861 ttcgggatgg tggatttgca gtccggcgag cgttgcgccg tagcggtgcc aatagtcttg
     7921 acgcaggtcg gacgcggcag attcggagag tttgaggcgg cgtgccatat agcgtgtcat
     7981 ggcgcggttg atgagtgtga agatgccggc atcggcattg tgcagcgtgt tgtcgaggtc
     8041 gaacagccac acggtcgggt tttcttgcat gttgaaccgt gaaaatttgt tagaatgtta
     8101 ttttacagcg aatagaggag gactcggaat gaaacggaaa atttggctgc tgccgctgct
     8161 ggcggtttcg gcatacctgc aggcgcagac ggaagtcagg ctggcggtgc ataagtcgtt
     8221 cagcctgccc aaagggctga ttgcgcgctt cgagcgggca aacgatgcga aggtgtcgat
     8281 tattcaggcg ggcggcgcga acgaaatgct caacaaactg attttgagcc gcgccaaccc
     8341 gattgccgac gcggtgtacg gtttggacaa cgccaatatc ggcaaggcgc gggaaatggg
     8401 cattttggcg gcagcgcaac ccgaatccgc ccccgtcgcg gtcgggctgc cttcggcttt
     8461 ggcggtcgat tacggctatg tgtccatcaa ttacgacaaa aaatggtttg aaggcaaaaa
     8521 gctgcccctg ccgcaaaccc tgcaggattt gacccgcccc gaatataaaa acctattggt
     8581 cgtgccgtct cccgccacgt cgtccccggg gctgggcttc ctgatggcga acatcagcgg
     8641 tctgggcgaa gaaggcgcgt tcaaatggtg ggcacagatg cggcagaacg gcgtgaaggt
     8701 cgccaaaggc tggagcgagg cgtattacac cgacttttcg cacaacggcg gcgcgtatcc
     8761 gctggtggtc ggttatgccg ccagcccggc ggcggaagtg tatttttcca aaggcaaata
     8821 cagcgagccg ccgacgggca acctgttttt aaaaggcggc gtattccgcc aggtcgaagg
     8881 cgcggcggtc ttgaagggcg cgaaacagcc ggaattggcg gcaaaactgg tgcaatggct
     8941 gcaaagtcgg gaagtgcagc aggcggttcc gtccgaaatg tgggtttacc ccgccgtcaa
     9001 aaacacgcgc ctgcccgacg tgttccgctt cgcccaagcc ccgacgcaca ccaccgcccc
     9061 cgcgcagcgc gatattgatg cgaaccagcg cggatgggtt tcccgttgga ttagaacggt
     9121 tttgaaataa aacaaacata ccgccccgca gggcttcata cggcattttt acacctgtgc
     9181 cgattacgcc gcacggggcg gatgttcgat caagaggaaa acaatggact tcaaacaatt
     9241 tgatttttta cacctgatca gtgtttccgg ttgggagcat ctggctgaaa aggcgtgggc
     9301 gttcgggctg aaccttgccg ccgcgctgct tatttttttg gtcggaaaat gggcggcgaa
     9361 acgcattgtc gctgtgatga gggcggcgat gacgcgcgcg caggtcgatg ccacgctgat
     9421 tagttttttg tgtaatgttg ccaatatcgg cttattgatt ttggtgatta ttgccgcatt
     9481 gggcagattg ggcgtttcca caacatccgt aaccgcctta atcggcggcg cgggtttggc
     9541 ggtggcgttg tccttgaaag accagctgtc caattttgcc gccggtgcgc tgattatcct
     9601 gttccgcccg ttcaaagtcg gcgattttat ccgcgtcggc ggttttgaag gatatgtccg
     9661 agagattaaa atggtgcaga cttctttgcg gacgaccgac aacgaagaag tcgtgctgcc
     9721 caacagcgtg gtgatgggca acagcatcgt caaccgttcc accctgccgc tttgccgcgc
     9781 ccaagtgata gtcggcgtcg attacaactg cgatttgaaa gtggcgaaag aggcggtgtt
     9841 gaaagccgcc gtcgaacacc ccttgagcgt tcaaaacgaa gagcggcagg ccgccgccta
     9901 catcaccgcc ttgggcgaca atgccatcga aatcacatta tgggcttggg caaacgaagc
     9961 agaccgctgg acgctgcaat gcgatttgaa cgaacaagtg gtcgaaaacc tccgcaaagt
    10021 caatatcaac atcccgttcc cgcaacgcga catacacatc atcaattctt aaacgccgtc
    10081 tgaaagggga gtgggaaaat ggacgcgctg cacaccatcg cccgaaacct gacgaaaaaa
    10141 cgtcaaaccg taagctgtgc cgagtcctgt acgggcggaa tgcttgccgc cgcattcaca
    10201 agcgttgcag gcagttcgca atggttcgac cagagttttg taacatacag caacaaagcc
    10261 aaagaagacc gcttgggcgt gttgcccgaa accctgctcg aacacggcgc ggtcagccgc
    10321 caaaccgtct atgagatggc gcgcggcgcg aaagccgtgg cgcaggcgga ttacgccgtc
    10381 ggtatttccg gcatcgccgg accgggcggc ggcagcgaaa gcaaacccgt cggcacggtt
    10441 tggttcgggt ttgcctttcc gggcggaagt tgcgaagcaa tgcgccgttt tgacggcaac
    10501 cgcgaatccg tccgcgcgca ggcggtcgcc ttcgcgttgg aacggttggc ggggctgatt
    10561 gaaaacggcg gcgatgctgt ctaaacaaaa tctccgtctg gacagaatcc ccgccggata
    10621 aaaaataccg tctgaaacgt tgcaggtttc agacggtatt ttgtcgggtt aggcggcggt
    10681 gcggcttatt tcactttact cttcaacgcg ccgtagcctg ccgcgtccat ttgttccagc
    10741 gggatgaatt tcaagctcgc gccgttgatg cagtagcgca gtccgccttt gtcgcgcggg
    10801 ccgtcgggga agacgtgtcc caaatgcgaa tcggcggcgt ggctgcgcac ttcggtgcgg
    10861 cgcatgttgt agctgaaatc atcgtgttcg gtaacggatt ttgcatcaat cgggcgcgtg
    10921 aagctcggcc agccgcagcc ggaatcatat ttgtcggcgg agctgaacaa aggttcgccg
    10981 ctgacaacgt ccacataaat gccgggtttg aacaaatggt cgtattcgtg gctgaaggca
    11041 tattcggtcg cgctgttttg ggtcacttgg tattgctctt cggtcagggt gcgtttgagt
    11101 tcggcgtcac tcggtttttt atacgttgcc gcgtcgaagc ctttgccttg cggggcggtc
    11161 ttggttttgc ccggcagcgg ttcgtcggct ttgcggatat cgatgtggca gtagccgttg
    11221 gggtttttaa tcaggtagtc ctgatggtat tcctcggcat cgtagaagtt tttcagcggc
    11281 tcgttttcaa caacgagggg cagttggtat ttttgctgct cgcgtttgag ggcggcggcg
    11341 atgacggctt tttcggcggg gtcggtgtag tacacgccgc tgcggtattg cgtaccggtg
    11401 tcgttgccct gtttgttgag gctggtcgga tcaacgacgc ggaagaaata ttgcaggatg
    11461 tcgtctaggc tgagtttgtc ggcatcgtag gtcactttaa cggtttcggc gtggcccgta
    11521 tggcggtagg acacgtcttc atagctcgga tttttcgtgt tgccgttggc gtagccggat
    11581 accgcgtcaa ccacgccgtc gatgcgttgg aaataggctt ccaagcccca gaagcagccg
    11641 ccggcgaggt aaatggtgcg tgtgttcatg atttttgaat cctttttctg agtgtcgggt
    11701 ttgtagaacg aatgtttcaa gctgcccaaa tcggcattcg ggtcgcggat taacgccaac
    11761 gcctgcgctt cgttgatgct gcctttgacg atgcgctgca cgtcgccgtc tttaccgatt
    11821 aacgcccacg aggggtaaac gctgatattc aggctttggg cgatcgtgcc gccgttgtcg
    11881 gttacgacgg gcagcttggg ataattcaaa ccggcatacc atttttggaa gtcgccgtct
    11941 tttttctcgt gcaaaaagcc cggagacgcg acggtaatca ggttggcgga gctgaatttt
    12001 gcatcttgcg cccatttttc ggtctgtccc aattcggaca aacacaaagg acaccagctc
    12061 gcccaaaatt taatcagcgt cggtttgtct tttttcaagt aaacactggc ggggcggttg
    12121 tccgcagttt tcaaagtgga taaagtgtgc ggcacggtcg cggctccggc atcgacgatt
    12181 ttgggcgaac aagcacccag cgcaagcagg cagccgaact tggcgcaaag ggaaaagaaa
    12241 gtacggtgtt tcattttgag tttcctgtgt ggacggtttg catgattaga cgtttgagat
    12301 gccgaaacct tacagctcgg attttcagac aaccttgccg cgtaaaatac gctacaatac
    12361 gccctgtttc aagtttctaa aattaaaagg aaaattcaat gttcagcttc ttccgtcgca
    12421 agaaaaaaca ggaaacgccg gctctcgagg aggctcaaat tcaggaaacc gcagcaaaag
    12481 cagagtccga aaccgatcaa gtaattgaaa atattaaaga agaagtcgaa tctttaacgg
    12541 aaagcgtcaa agagcaggtc gaatcggctg ttgaaaccgt cagcggtgcg gttgaacaag
    12601 taaaggaaac cgttgccgag atgctgtctg aagcagagga agcggcggaa aaagcagcgg
    12661 aacaagtcga agcggcaaaa gaagccattg ccgaaaccgt cggcgaggct gtcgggcaag
    12721 ttcaagaagc cgttgcaaca actgaagaac acaagctcgg ttgggcggcg cgtttgaaac
    12781 aaggtttgac caaatcgcgc gacaaaatgg cgaaatcgct ggcgggcgtg ttcggcggcg
    12841 gacaaatcga cgaggatttg tacgaagagc tggaaaccgt gctgattacc agcgatatgg
    12901 gcatggaagc caccgaatac ctgatgaaag acgtgcgcga ccgcgtcagc ctcaaagggc
    12961 tgaaagacgg caacgaattg cgcggcgcgt tgaaagaagc cttgtacgac ctgattaagc
    13021 ctctggaaaa accattggtc ttgcccgaaa ccaaagagcc gtttgtcatc atgcttgccg
    13081 gcatcaacgg cgcgggcaaa accacgtcta tcggcaaact cgccaaatat ttccaagcgc
    13141 agggcaaatc cgtattgctg gcggcaggcg acactttccg cgccgccgcc cgtgagcagc
    13201 ttcaggcatg gggcgaacgc aacaacgtta ccgtgatttc gcaaaccacg ggcgattccg
    13261 ccgccgtgtg cttcgatgcc gtccaagccg ccaaagcgcg cggcatcgac atcgtgcttg
    13321 ccgacaccgc cggccgcctg cccacgcagc ttcatttgat ggaagaaatc aaaaaagtga
    13381 aacgcgtgct gcaaaaagcc atgcccgacg cgccgcacga aatcatcgtc gtgcttgatg
    13441 ccaacatcgg gcaaaacgcc gtcaaccaag tcaaagcctt tgacgacgca ttagggctga
    13501 ccggtttaat cgtgaccaaa ctcgacggca cggcaaaagg cggcatcctc gccgcgcttg
    13561 cttccgaccg ccccgtcccc gtccgctaca tcggcgtggg cgaaggcata gacgatttgc
    13621 gcccgtttga cgcgcgcgcg tttgtggacg cactgctgga ttgagccgaa atgccgtccg
    13681 aaaacagcag accgatgccg tcattcccgc gcaggcggga atctagaacc atcggcacgg
    13741 aaacttatcg ggtaaacggt ttcttgagat ttttcgtcct ggattcccac tttcgtggga
    13801 atgacggaat gtaggttcg
//
LOCUS       DAWKST010000043        13581 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.043, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000043 DAWKST010000000
VERSION     DAWKST010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13581
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.043"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(63..623)
                     /locus_tag="WJL87_000872"
     CDS             complement(63..623)
                     /locus_tag="WJL87_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ7799321.1"
                     /translation="MNMKTLLALAVSAVCSVSVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
     gene            complement(1040..2080)
                     /gene="mutY"
                     /locus_tag="WJL87_000873"
     CDS             complement(1040..2080)
                     /gene="mutY"
                     /locus_tag="WJL87_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ7799322.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVRQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPAHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            2243..3284
                     /locus_tag="WJL87_000874"
                     /pseudo
     CDS             2243..3284
                     /locus_tag="WJL87_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            complement(3307..3525)
                     /locus_tag="WJL87_000875"
     CDS             complement(3307..3525)
                     /locus_tag="WJL87_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799323.1"
                     /translation="MPSEALSSLRRHFAAWRRSRHTRQHHSDILPLKCPSAFRIIKIT
                     FSSFDSSRTAHTTRLKKCRLKRIQTASA"
     gene            complement(3540..4178)
                     /locus_tag="WJL87_000876"
     CDS             complement(3540..4178)
                     /locus_tag="WJL87_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ7799324.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAIDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     RNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(4359..6194)
                     /gene="edd"
                     /locus_tag="WJL87_000877"
     CDS             complement(4359..6194)
                     /gene="edd"
                     /locus_tag="WJL87_000877"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEZ7799325.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVSGELNVLINETE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
     gene            6710..8155
                     /gene="zwf"
                     /locus_tag="WJL87_000878"
     CDS             6710..8155
                     /gene="zwf"
                     /locus_tag="WJL87_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ7799326.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERLAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAARGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALESRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
     gene            8435..9130
                     /gene="pgl"
                     /locus_tag="WJL87_000879"
     CDS             8435..9130
                     /gene="pgl"
                     /locus_tag="WJL87_000879"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ7799327.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            9111..10097
                     /locus_tag="WJL87_000880"
     CDS             9111..10097
                     /locus_tag="WJL87_000880"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ7799328.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            10147..10995
                     /gene="hexR"
                     /locus_tag="WJL87_000881"
     CDS             10147..10995
                     /gene="hexR"
                     /locus_tag="WJL87_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ7799329.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            11116..12762
                     /gene="pgi"
                     /locus_tag="WJL87_000882"
     CDS             11116..12762
                     /gene="pgi"
                     /locus_tag="WJL87_000882"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ7799330.1"
                     /translation="MKHFHDLPAWPKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDESAVAKHFVAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPAILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGPQKHDSSTERLIRLYLQTNRK"
     gene            12776..13327
                     /locus_tag="WJL87_000883"
     CDS             12776..13327
                     /locus_tag="WJL87_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ7799331.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHVCSAAM
                     ALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEVDRVSEWEERYKLSRSEFEQFWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKFSELACKTAETEARLEELH
                     NRKKALIDEMAREADKKELPKRL"
BASE COUNT         3287 a         3455 c         3688 g         3151 t
ORIGIN      
        1 ctaaatatct agcaaaaaag tgcggtcaaa attcaccgca cttttcgttt cgaacaatcg
       61 aattatttaa tcacgccaca tgccatacgt gggccgccac cgccaagtgg agctggatga
      121 tcggagtgat tatcaccacc cgtgtggatc ataatagagt gaccgcgaac atcatctaaa
      181 tgtttaagac gtggtgctaa aacaggattt gttgctgtgc catcatgcaa tacagttaat
      241 gcaggtaaat cacctaagtg tgcatcatct tgccatgggt aaccatgttg ttttgcacct
      301 ttaggatccc agtgaccgcc tgcgcctaaa ccagctgtca atttaccttc tttttctttt
      361 ggctcacagc ttgggttttc atggatgtgg aaaccatgta agccttcgct taatccttgt
      421 aaatcagggg taaacacaag accatagtta gattcagtaa tagtcactgt acccacatct
      481 ttgttaccgt ttactggatc aagttgttgc actttcactt caatagaagc acctttaggt
      541 atcgtattat gctcgtgtgc ttgcgcaaca ctaactgaac atactgcact aaccgctagt
      601 gctaataagg ttttcatatt cattgcaatc tcctttattg cagtaaaaac tcgcgactat
      661 tttagtcaat tttgttttaa aagccgataa atttttacga aaattttaca ttaattgtta
      721 tttgcagaaa attagaaccc gttatcacgt cttggataaa tgccaaaacc attgtgattc
      781 cgagaattaa aaagaaaaat ttcggaatcc tcatcctttt ctgaaacctg attgcggtat
      841 ccatggcttt gtctttgtca tttaagtttg tgttcatgtt tttgaccttc tttgcttttt
      901 aaatcctttt taaattgccg acttcttccg ttccaaatgt cccatttgaa cattatcccg
      961 ccgcctaaaa tacgacccaa attcatcggt tgttcttgct gcgatacctg caaacaacag
     1021 ttcaatgggt ttattttgtt tattctaacc tattaccgtt taaataaatt tccaaaggct
     1081 tgggcaggcc gtaatctttc aaatgcgccg gctttatcca aatgccgtct gaagggcttt
     1141 cagacggcat ttgtccttca aacggcgtaa tcatcagcag ccggtgcgtc agacggtggg
     1201 tcagggcggt ttgttcgtcc atatctgcca tggtcaggga gaatttggcg gcaaagtcgg
     1261 aaagcccgtt caaactttca aaacacggca cgcaatacag cccgccccaa atgcctttgg
     1321 cggggcgttt ttccagcaaa atcgcgccgt cccggttgcg gacaatcagc cagtaaagcg
     1381 gcagggtcgg tacttcggcg gcggtttttt tgcgcggcag ctcggcggtg cggttttgct
     1441 tttttgcttc gcagatgtcc gccatcgggc attggtggca caagggtttc gtccgtttgc
     1501 acacggtcgc gcccaaatcc atcaaacctt gtgtataggc aggcatatcg gcgttttcag
     1561 acggcagcag gctttcggca agtgtccaga gcgagttttc aaattttttg tcctgcggat
     1621 tgccgtcgcg ggcgaacacg cggcagagta cgcgtttgac gttgccgtcc aaaatggttt
     1681 cgcggcggtt gaaggagaag gcgcaaatgg cggcggcggt gcttctgcct acgccgcaga
     1741 gcgtttccaa gtctttgcgt tccgatggaa acgtgccgcc gaattgcctg acgacttgtt
     1801 gcgcggcttt gtgcaggttg cgcgcgcggc tgtaatagcc caagcccgcc cacaacgaca
     1861 acacttcgtc ttgcggcgcg gcggcaagcg tctgaacggt cgggaatttt tctaagaaac
     1921 gcggatagta gtccaacacg gtggcgactt gcgtttgctg gagcatgatt tcggaaagcc
     1981 agacgcaata agggtttttg acctgccaag ggaggtggtg gcgaccgtgt tgtttttgcc
     2041 agcggatgag ccgttcggag aaggggatgg gtgtgttcat taatatcaat cggtggtttt
     2101 atttatattt aaaacggtat gttattgtat aacattgtga aaataattct tattgactta
     2161 ttttttgtag gggcatataa ctcatataaa gaaactttat tgcggtattg aaattattta
     2221 tcaacaagca aggagtatca gcatgaaagc aatggtttat cacggcgcaa acgacatccg
     2281 ttttgaagaa aaaccccgtc cgcagattat cgatccgacc gatgcggtgg tgaaaatcgt
     2341 caaaaccacg atttgcggta ccgatttggg tatttggaaa ggcaaaaaaa cccgaagtcg
     2401 ccgacggtcg tattctcggt catgaaggca tcggtattgt agaagaagtc ggcgatgctg
     2461 taaaaaacat caaagtcggc gataaagtca ttatttcatg cgtcagcaaa tgctgtactt
     2521 gcgacaactg caaaatccaa ctttattcgc attgccgcaa cggcggctgg attttgggct
     2581 acatgattga cggcacgcag gcagaatacg tccgcacgcc ttatgccgac aacagcctcg
     2641 tgccgctgcc cgacaacgtc aacgaagaag tcgccctgtt gttgagcgac gctttgccga
     2701 ccgcacacga aatcggcgtg caatacggcg atgtcaaacc cggcgacacc gtattcatcg
     2761 caggtgccgc tcctgtcggt atgtccgcct tgttgaccgc ccaactgtat agccccgccg
     2821 ccattatcgt gtgcgatatg gacgaaaacc gtttgaaact ggcgaaagag ttgggcgcga
     2881 cccataccat cagccccgct tccggcgacg tctccaaaca agttttcgcc atcgtcggcg
     2941 aagacggcgt agattgtgcc atcgaagcgg tcggtatccc tgcaacttgg aatatgtgcc
     3001 aagacatcgt gaaacccggc ggccatatcg ccgttgtcgg cgtacacggt caatccgttg
     3061 atttcaaact ggaaaaactg tggattaaaa agcttgccat cactaccggc ctggtaaacg
     3121 ccaataccac cgaaatgctg atgaaggcaa tttccagcag ctccgtcgat tacaccaaaa
     3181 tgttgaccca ccgtttcaaa ttcagcgaat tggaaaaagc ctacgacgtg ttcaaacacg
     3241 ccgccgaaaa tcaagcgatg aaagtggttt tggaagcgga ttaatcggcg ttgaatcagt
     3301 tgttttttaa gcggatgccg tctgaatgcg tttcagacgg cattttttca gccgcgtagt
     3361 atgggctgtc cggctgctgt cgaatgaact gaacgttatt tttattatgc ggaaggcgga
     3421 tgggcatttt aggggcagta tgtcggagtg gtgctgccgt gtatgccgac tgcgccgcca
     3481 agcggcaaaa tgccgtctga gactggaaag ggcttcagac ggcattttta cgatgcggtt
     3541 caggctttgg gtttcaatgc cgccgcttct ttggcgagac gggtgatggt gtcccagtct
     3601 ttgtttttca cggcttcttt cggcgtcagc catgagccgc cgacgcacag gacgttgggc
     3661 agtgccaagt aatcgggcgc ggtggcgagg ctgatgccgc ctgtcgggca gaagcgaaca
     3721 tcggcgtaag ggccgtacag ggctttgagc atggctttgc cgccaacgac ttcggcaggg
     3781 aagagtttga gggtgtcgat gccgtgttcc aaagccagtt ggatttcgcc cggggtggca
     3841 acaccgggaa tcagggggat gccgctgttg cggccggctt tggcgaggga ttcatgcagc
     3901 cccgggctga tggcgaaaac cgcgcctgcg tcttcgacgg ctttaagctg ttcgggattg
     3961 gttaccgtac ctgcgccgac gatggcgttg ggcacttctt tggcaatcag gcggatggca
     4021 tcgaggccga caggggtgcg cagggtgatt tcgagggtag ggatgccgcc ttcgacaagg
     4081 gcgtgggaca aatcgatggc ggtgcttaag tcgtcaatcg ccattaccgg cacaactgcg
     4141 ccggcggtca aaatttcgcg gggggtcagt ttggacattt cggttctccg ggtggaatgg
     4201 ggtattttat taagatggga caggtcgtag gtcggattca agaaaccgac atcttggacg
     4261 gctgtgtcgg attcgagaat ccaacctacg tctatcttaa aacacttgaa taatttttca
     4321 gacggccttc ggtctgaaaa ggccgtctga aacgcgcatc aggcaaattc gccgccgaag
     4381 ctcatcgcgc cggtttcggc gctgctggtc atgctgcgga agttggcgaa aagttcgcgg
     4441 ccgcagcctt gttggttcgc gcccaagtcg atgctttcga cttcgcgggc attccattcg
     4501 gtttcgttaa tcaggacgtt gagttcgccg ctaacggagt cgaagcggat caggtcgccg
     4561 gtacggattt tggcgatgtt gccgcccatc agggcttcgg gcgtcatgtg gatggaggcc
     4621 ggaactttgc cggatgcgcc ggacatacga ccgtcggtca gcagtgccac tttgaagccg
     4681 cggtcttgca ggatgcccaa aggcggggtc agtttgtgca attcgggcat accgttggca
     4741 cgcgggcctt ggtagcggac gacgcacaca aaatcgcgtt ccaactcgcc gcgttcaaac
     4801 gcagccaaca cttcgcgttg gtcgttgaac acgatggcag gcgcttcaat aatgcggcag
     4861 ccttcgcgca cggcggacac tttaatcacg ccacgtccga tgttgccttt catcaggcgc
     4921 agaccgccgt cgggggagaa cgggttgtca gctttacgca ggatgtcgtc gttgccgctg
     4981 gtttcggggg cttcgcgcca ttcgagtttg ccgtcgatga ggaaaggctc tttggtgtag
     5041 tggcgcatac cgtgtccgac gacggtatcg acatcgtcgt gcaacaggcc tgcattcagc
     5101 aattcgcgga taacgaaagg cagtccgccc gctgcggtaa agtgattcac gtcggccttg
     5161 ccgttcggat aaacgcggat gagtagtggg atgatggagg aaatttcgtc gaagtcgtcc
     5221 cagttcaaaa tcacgccggc cgcacgcgcc atagcgacga ggtgcatggt gtggttggtc
     5281 gaaccgccgg ttgccatcag gccaatcaag gcgttgataa aggatttttc ggtcaacatt
     5341 tcgcccaaag gtttaatcgt gccgtttttg atgccgcgcg cgaggtgtcc ggcggcgtag
     5401 cgagtcagcg cttcgcgcag gtcggtgtaa gggtggacga aggcggcggc aggcaggtgc
     5461 acgcccatca tttccatcat catttggttg gagtttgccg tgccgtagaa agtgcaggtg
     5521 cccgggctgt ggtaagaacc catttcgctt ttcaaaagtt cgttgcgtcc gaccttgcct
     5581 tcggcgaaaa gctggcgggt gcgggctttt tctttgttgc cgataccgct ggacatcggg
     5641 cctgcgggga cgaagatacc cggaatatga ccgaacgaaa gcgcgcctat catcaaaccc
     5701 ggaacgattt tgtcgcatac gcccataaac aggctgccgt caaacatttg atgcgacagc
     5761 ccgacggcgg tactcatggc aatcacgtcg cgggagaaca gcgacagttc catgccggcg
     5821 tagccttgcg tgatgccgtc gcacatggcg ggcgtgccgc cggcgacttg ggcggtcgcg
     5881 ccgtttttct gcgcttcgtc tttgatttgg tcagggaagt ctttaaacgg ctggtgtgcg
     5941 gaaaccatgt cgttgtaggc ggtgatgatg cctaagttgg ggacggtttc ctgaagcatt
     6001 tcgattttga tacttttagg catggcagca tagccgtgtg ccaagttgct gcagccgagc
     6061 tggttgcgct ctaagcgtcc catttgtttg gcactgcgga tcttcgccag atatttttca
     6121 cgcgtcggac ggctgcgttc aataatgcgc ccggtgattt cggcgagttt ggagtggata
     6181 ggagtagtgt tcacggtcgg tctcctgtcg gaacgggggg tattggggat ttgtttgtgc
     6241 gtttctgatt tttgtatcgg gtattcagac ggtgagtata gtggattaac aaaaaccagt
     6301 acggcgttgc ctcgccttgc cgtattatct gtactgtctg cggcttcgtc gccttgtcct
     6361 gatttttgtt aatccactat attttaaaag gtataccgtc tgaaagtggg aagcgggtgt
     6421 tattatagta caaaatttgt aattttgtta caaaaaaatc agacatcgaa aagaaatttg
     6481 atttaaatcg cagaatcttc cgattgttgt aacggaatag gggaaacctg cccgattttt
     6541 ggactaggtc aaataaattg taaaaatggg aatagacaaa aagtgtagcg gtgtgtagta
     6601 ttactactca ttatgccgcc tgcctttgtt tccgatattg cgatgcagcg tgatggggga
     6661 aacaggtttc agacggcatc gaaaaattct ttcctgacga gataatacga tgagtacaca
     6721 aacaaatttt gatttggtgt tgttcggtgc gaccggcgat ttggcgatgc gcaagctgtt
     6781 gccctgtctg taccaggcgc atgttgccgg tctgcttcat cccgaagggc gtattttagg
     6841 ggtcagccgc agcgagttgg ataccgaagg ttttctggcg aaggtggaaa ccagttccaa
     6901 aatccatgtg aaagaaaatt tttccgatga ggcttgggca tcgtttgtcg aacgtctcgc
     6961 atatctcaaa gtcgatgtta cgcagccgga cgattttgcc gctttgggcg atttggtcaa
     7021 agcgcgcaaa gaaaccgaca atgtcgtcat ctatctttcc accgcgccga aattcttcgc
     7081 gcaaacatgt gaaaaccttg ccaaaatcgg tttgaacgcc gacaacgtgc gtgtggtttt
     7141 ggaaaaaccg ttgggtacgg atttggcttc ttcccagcaa atcaataccg atgttgcgcg
     7201 ttatttcaaa gaagggcaaa tttaccgcat cgaccactac cttggcaaag aaagcctgca
     7261 aaacctgctt gcgctgcgtt ttgccaatgt aatgttcgag ccgctgtgga acaataaata
     7321 catcgaaagc gtgcagttga ccatcgccga gcagttgggc gtggaagagc gcggcgagtt
     7381 ttacgacatt accggcgcgt tgcgcgatat ggtgcaaaac catttgatgc agatgctgtg
     7441 catgactgcg atggaagccc ccgccagctt ggatgccgac gcggtgcgcg atgaaaaagt
     7501 caaagtcatc aagtcattga agccgctgac cgtcgagtct gtcaatgaaa acgtcgtgcg
     7561 cggacaatat accgccgcca gaggcatgaa cggctatctt gaagaaatca acgttccgca
     7621 agacagcttt accgaaacct acgtcgccat taaagccgaa atcgaaaacg aacgctggaa
     7681 gggcgttccc ttctacctgc gtaccggcaa acgcatggcg ggcaaagtgg cggaaatcgt
     7741 tttgaacttc aaagatttga acagccatat ttttgaaggc agccgcaccg cgcccaaccg
     7801 gctcgttatc gagttgcaac catatgaatc cgtgcgcctc tatacgcaga tgaaaacccc
     7861 gggggcagga aataaggtcg aaaccgtgcc gctggcaacc gatttgggca aagcattgga
     7921 aagccgccgc gcggaagctt acgagcgcct gctgctggat gtgattaacg gcaaactcgc
     7981 tttgtttaac cgccgcgacg aactcgaagc cgcgtgggaa tatgtgatgc cgattttgga
     8041 aaactggaca aataacacca cgccgccgca cggctacggc gcacactcgt gggggcctga
     8101 agccgcgcgc gaactattgg cgcgcgacgg acacaagtgg cacgaagagc agtaatacaa
     8161 taatgcgttc agacggcatg gggtttgaaa tgccgtctga acgtaagtaa agcagtatag
     8221 tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacaaat agtacggcaa
     8281 ggcgaggcaa cgctgtactg gtttaaattt aatccactat aaaaagcagt cccgatattt
     8341 ggtatcaaaa gcataaacaa ctgttcggcc gacatattgc tcaacccgtt tccataacgg
     8401 aatatgccgt ctgaaataaa aaaaggacac aaatatgttt gtttggcacg aatacgaaaa
     8461 tgcggcagaa gcggcgcagt ctttggctga cgcagtggcg gatgctttgc agggcgcact
     8521 ggacgagaag ggcggtgcgg tgttggcagt ttccggcgga cgttcgccga ttgcattttt
     8581 caacgccctg tcgcaaaaag atttggattg gaaaaacgtc ggcatcacct tggcagatga
     8641 acgcatcgtg ccgaccgtcc acgccgacag caataccggt ttggtgcgcg aatacctgtt
     8701 gaagaacaaa gcggaagcgg caatgtggat tcctatggtg gaagacggaa aaactgaaac
     8761 cgaattacat cccgatgctg ttgtcgatta tgcactgaaa cattacaaac agcccgatgt
     8821 tttggttttg ggtatgggaa acgacgggca tacggcttcg attttcccga aagctccgca
     8881 gtttcagacg gcaatcgacg gttcggcagg tgtcgcgctg gtgcatacca cgcccgttac
     8941 cgcgccgcac gagcgcgtca gtatgacctt ggatgcgatt gcccatacgg ggcatgtgtt
     9001 tttggcgata cggggcgaag agaaaaaagc cgtgttcgac caagccgcac aaggcgaaaa
     9061 ccgcgaatat ccgatcaacc tcgttttgaa ccatcaagga gtgaactgcc atgtcttcta
     9121 cgccgaataa acaagccgga tatccccgac tggtcgccga tatcggcggg acgaatgcac
     9181 gctttgcgct ggaaaccgcg ccgcgcgtca ttgaaaaagc cgccgtgctt ccgtgtaaag
     9241 actacgatac ggttaccgat gcggtgcgtg cctatctgaa tcaaagcggt gcaacagccg
     9301 tacggcacgc ggcatttgcc atcgccaacc cgattttggg cgactgggtg cagatgacca
     9361 accaccattg ggcgttttcc atcgaaacca cccgtcagac tttggggctg gacaccctca
     9421 tccttttgaa cgactttacc gcgcaggcat tggcggtaac gcagacttca agcaaagacc
     9481 tgatgcaggt aggcgggcaa aagcctgtcg aatttgcccc caaagccgtt atcggccccg
     9541 gtaccggcct gggcgtgagc ggattggtgc acagccacgc aggctgggtg gctttggcgg
     9601 gcgagggcgg gcataccagt ttcccgccgt ttgacgatat ggaagtgctg atttggcagt
     9661 acgccaaaaa caaatacggc catgtttccg ccgaacgctt tttgagtggc gcgggcttga
     9721 gcttggttta cgaggctttg gctgcaaaac agaaagccaa acccgccaaa ctgatgccgt
     9781 ctgaaatcac ggaaaaggct ttgagcggcg cgtcgccttt gtgccgtcag actttggaca
     9841 tcttctgcgc catgctcggc acggttgctt ccaacctcgc cctgacgctg ggcgcgcgcg
     9901 gcggcgtgta cctgtgcggc ggcattattc cccgcgtgtt ggaatatttc aaaacttccc
     9961 cgttccgcag ccgtttcgag aacaagggca ggtttgaagc atatcttgcc gcgattcccg
    10021 tgtatgtcgt cttgagcgag ttccccggaa tttccggtgc ggctgcggct ctcgacaacc
    10081 atttgagaaa cgtttaacca cagcggctcc ttgcagcggg gctgcattat cgaagggcat
    10141 atcattatgt taagcaaaat cagcgaatca ctggcaaacc tttccggtgc ggaacgcaaa
    10201 gtcgccgaat gtgcattggc ggaacccaaa tggttcgttc atgccgccgt tgccgaaatt
    10261 gccgaacgtg cgtccgtcag ccagccgacc gtcatccgat tctgccgcag cttgggttat
    10321 aaagggctgc ccgagttcaa gctcgccttg tccgccagca tcggtcatga gggtatgccc
    10381 tatgtccacg aagaactcaa tgccgacgac gatatggcaa gcgtggtcga gaaagtgttg
    10441 ggcaatgccg ccgcctcgct gttgggcgaa cgccgcttcc tgaaagagtc ggagctggaa
    10501 aacgccattg ccacgctgat gcacgcccgt cgcgtcgagt tttacggtgt cggcaattcc
    10561 ggtatcgttg cgcaggacgc gcaacacaag tttttccgtt tcggaatgtc cacggtcgcc
    10621 tatgtcgata cgcacacgca gctgatggca gcatccgttt tgagcgatca ggatgttttg
    10681 gttgccattt ccaacacggg ttcgtctatc gaacttttgg atgcggtcag catcgccaaa
    10741 gaaaacggcg cgtccgtcat cgcactgacc cgcaacgatt cgcctctggc gcaacttgcc
    10801 gactgcgtgt tgagcgttgc cacacaggaa aatgccgaac tctacacgcc catggtttcc
    10861 cgcctcttgc agcttgccgt catcgacatt ctcgccatcg gacttgccct gcgcttgggc
    10921 gatgctgcca gcctgcaatt gcagaaaagc aaaaaaagca tacacaacaa gcacatcgat
    10981 tacgacaaag attgaccttc agacggcatc ccacaaatgc cgtctgaaat gccgaacaac
    11041 ggccgtcggc ggcttgcggc agtttccggc agccttttca tcccacaaca aaaaacctca
    11101 ttcaggagca tatagatgaa acactttcac gacttacccg catggccgaa attgtggaac
    11161 cactttgacg acagcaaaac attgcatatg cgcgaaatgt tcgagcaaga cccgcagcgt
    11221 gcggaacgct actggctgca agtcggcgga ctgacgctgg actactccaa aaaccgcatc
    11281 aacgacgaaa ccatgtcgct tttgttcgag ttggcgcgtg aagcaggcgt gccggagcgg
    11341 atgcggcaga tgttccacgg cgaaaaaatc aatacgacgg aaaaccgcgc cgtcctccac
    11401 gtcgcccttc gcaaccgcac caattcgccg attgtggttg acggtgaaga tgtgatgcca
    11461 aaagtcaacc gcgttttgca acgtatgggc gaatttgcac acgaagtccg cagtggaagc
    11521 tggctgggct ataccaacca agtcattacc gacgttgtca acatcggcat cggcggatcg
    11581 gatttgggcc cgttgatgat gtgtaccgcg ctcaaacctt tcggtcatcc acgcctcaat
    11641 atgcacttcg tctccaacgt ggacggctcg caactgcgcg acgtattgtc caaagtccac
    11701 cccgaaacca cgctgttcat tatcgcctcc aaaaccttta ccacgcagga aacgctgacc
    11761 aacgcgctga ccgcgcgcga atggtttttg aatcatgcgg gcgatgaaag cgccgtcgcc
    11821 aaacatttcg ttgctgtttc caccaaccaa aaagccgttg ccgaattcgg catcgacacc
    11881 gccaatatgt ttgaattttg ggactgggtc ggcggccggt acagcctgtg gtccgccatc
    11941 ggattgccga ttatgctgta tctcggcgaa gaaaacttca tcgaaatgct caacggcgcg
    12001 cacctgatgg accaacattt catcaacact ccgctcgagc gcaacctgcc cgccatcctc
    12061 gcccttatcg gcatctggta catcaactac tacggcggcg gaagccacgt catcgcacct
    12121 tacgaccaac acctccaccg cctgcccaaa ttcatccagc agctcgatat ggaaagcaac
    12181 ggcaaacagg ttacgttgga cggcaaagca gtcggacacg aaacctcgcc gattatttgg
    12241 ggcgaaacgg gcatcaacgg ccagcacgcc ttcttccaac tgctgcacca aggcacgcac
    12301 atcaccccca tcgacctcat cgcctcgctt gaaaaacgca gcaacctgcc cggacaccac
    12361 gaaatcctgc ttgccaacgt cttcgcccaa gcagaagcct ttatgcgcgg caaaaccccc
    12421 gacgaagtcc gcgccgaact caaagcgcag ggtatggatg aggcgcgtat cgaagagctg
    12481 gttccgcaca aaaccttctc cggcaaccgc ccgaccaacc tcatcctgat ggataaggtc
    12541 aacccccgca atatgggcag cctgattgcc atgtacgaac acaaaacctt cgtacaaggc
    12601 atcatttggg gcatcaacag cttcgaccag tggggcgtgg aactcggcaa acaactggct
    12661 aaaaccattt tgggcgaact gaccggcgaa accgggccgc aaaagcacga cagttcgacc
    12721 gaacgcctga tcaggcttta cctgcaaacc aaccgcaaat aaaacctgcg gaaaaatgcc
    12781 gtctgaacgc cgaccgtttc aaacggcatt tttatcgaac aggaaaaccg tcggtaaact
    12841 tgcagagcgt gtgcaatccc gatatgatgg tttgcataaa tttaaacatg tatgttccgc
    12901 agctatggca ctgattaaag agccgttgga caaagcgaaa caaaggaacg aagaacttga
    12961 agcggcagaa gaagcggcgg cgcaggaggc attgggtcgg gagcaggaag tcgaccgcgt
    13021 atccgaatgg gaagaacgct acaagctgtc gcgcagcgag ttcgagcaat tctggaaagg
    13081 attgcctcaa accgtacaga ataagctgca agcctcacag aaaacatgga aaagcgggat
    13141 ggataaaatc tgtgccaaca atgcgaaagc tgaaggtgaa acgccaaacg gcataaaatt
    13201 cagcgaactg gcatgcaaaa cggcggaaac cgaagcacgc ttggaagagc tgcacaaccg
    13261 taaaaaagcc cttatcgacg aaatggccag ggaagcggac aagaaagaac tgccaaagcg
    13321 gctctgaaca gcgcggttca ggcactgccc gccgatattg ccgaaaccgt catgcccgag
    13381 taccgcaact gacaaaacgg tttgaatgcc aaatgcgccg acggcgacga atacggcgtg
    13441 gcacaatctg actgccgtac cagagaaatc aatgcgaaaa ccaaagaaat ccaaggttat
    13501 ctgattgact gaaacttgga tgcgggaatg tcggcggctt ttgcgttttt gtcgttttta
    13561 tagtgaatta aatttaaacc g
//
LOCUS       DAWKST010000044        13568 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.044, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000044 DAWKST010000000
VERSION     DAWKST010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13568
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.044"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(77..152)
                     /locus_tag="WJL87_000884"
     tRNA            complement(77..152)
                     /locus_tag="WJL87_000884"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(117..119),aa:Asn,seq:gtt)
     gene            complement(343..1095)
                     /locus_tag="WJL87_000885"
     CDS             complement(343..1095)
                     /locus_tag="WJL87_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ7799332.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETRVFECGVMPHPNLTECEAGQF
                     DRDFWDNTGCAPEEYVRIRRAIRLLEVRYPDSLNELVCAAIATPLGEMLAVFGNKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            1406..1930
                     /locus_tag="WJL87_000886"
     CDS             1406..1930
                     /locus_tag="WJL87_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEZ7799333.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQLLSA
                     PYGFGCSGGNAPPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHWVVADIPADVRRRN
                     AASLQLSRCANIADDQSAAISAVISLRICRIRLTPSYTAKPMPSCCNHANTPQSAASA
                     TLCGTFSSASTAAA"
     gene            complement(2019..2298)
                     /locus_tag="WJL87_000887"
                     /pseudo
     CDS             complement(2019..2298)
                     /locus_tag="WJL87_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246300.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(2670..3815)
                     /gene="dapE"
                     /locus_tag="WJL87_000888"
     CDS             complement(2670..3815)
                     /gene="dapE"
                     /locus_tag="WJL87_000888"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEZ7799334.1"
                     /translation="MTETQSLELAKELISRPSVTPDDQNCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPAERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARDELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNKYFPPTSFQISNINGGTGATNVIPGELSVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTQAGKLTDVARAAIAETCGIEAELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLDDIPKLSAVYEGILARLLAGNAV"
     gene            complement(3929..4549)
                     /locus_tag="WJL87_000889"
     CDS             complement(3929..4549)
                     /locus_tag="WJL87_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenosine tetraphosphatase"
                     /protein_id="HEZ7799335.1"
                     /translation="MLTPKSCDLFNIPFFQFAQLKKYRPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYAQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPITLPDYNRWLDNFDTEKYASFDMWHGAESEYDDYRTVAQQNESDR
                     RLQNDEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(4583..5092)
                     /locus_tag="WJL87_000890"
     CDS             complement(4583..5092)
                     /locus_tag="WJL87_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ7799336.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQIAQYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHIGLHDN
                     WAAVSAEFAKLEADNAYVPDEEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(5187..5738)
                     /gene="azu"
                     /locus_tag="WJL87_000891"
     CDS             complement(5187..5738)
                     /gene="azu"
                     /locus_tag="WJL87_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ7799337.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPAKLADG
                     EYKFACTFPGHGALMNGKVTLVD"
     gene            complement(6019..6441)
                     /locus_tag="WJL87_000892"
     CDS             complement(6019..6441)
                     /locus_tag="WJL87_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ7799338.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLAPKPEKPPKTNT
                     LHLSKAALESFGSAAVKLEKRHPELAEALANLVRRHGA"
     gene            6572..9322
                     /gene="secA"
                     /locus_tag="WJL87_000893"
     CDS             6572..9322
                     /gene="secA"
                     /locus_tag="WJL87_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ7799339.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMQEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            9337..9489
                     /locus_tag="WJL87_000894"
     CDS             9337..9489
                     /locus_tag="WJL87_000894"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799340.1"
                     /translation="MPSEHPAPVSDGILPEPPHPTAIPKNPDFVPSVPKTDIPSAPHH
                     DSIRLH"
     gene            9467..11239
                     /locus_tag="WJL87_000895"
     CDS             9467..11239
                     /locus_tag="WJL87_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ7799341.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMVVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMLTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAESIKNHAAVPETAQV
                     LEYMRGSPYEETITRIFHSTHQSEEMNSSSEEDCENFQIGMKKLLNELKYSQIEALKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            11427..13355
                     /gene="rpoD"
                     /locus_tag="WJL87_000896"
     CDS             11427..13355
                     /gene="rpoD"
                     /locus_tag="WJL87_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ7799342.1"
                     /translation="MSKNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
BASE COUNT         3466 a         3847 c         3345 g         2910 t
ORIGIN      
        1 ctatcttgcc gcattccgaa cgccgtacgt gcaaatatga aaaagcccga acattcaagt
       61 tcgggcttca aaattctggc tccccgacct gggctcgaac cagggacctg cggattaaca
      121 gtccgtcgct ctaccgactg agctatcggg gaatggggtg tattatagcg tccgaaaaaa
      181 atgtgtcaat ccttaatttt ggaaaaatgg gcgacaaaac gacaagcata tgaatcagaa
      241 agacattaag accgatgcct taaaaggatt gccgttgtat gaatttccac agccgtcatc
      301 acaccatatt taagcccgat gagccgttct gccctccccc gcttaaaaca atgccgtctg
      361 aacttcgccg cgttccaaag ccagtaaaaa ctgtttgcgg ttcaacccgc ccgcgtagcc
      421 ggtcagtttg ccgtcgctgc cgatgacgcg gtggcaggga atcaggatag atactttgtt
      481 ctgcccgttg gcggcggcaa cggcgcggac ggctttgggg ttgcccaaac gctgcgcctg
      541 ctccttgtag ctgcgcgttt cgccgtaagg aatcgccaag agcgcgtccc atgcctgctt
      601 ttgaaactcg gtgccaatct gctccaaagg cgtggcaaag gttttcagac gacccttgaa
      661 gtataagtcc aattcctgcc gcaaaagttg cgtccgctca tcctcccgaa acacaaaccg
      721 tccgcgcaag gctttttgga cggcggcaat ttcctgttcc aaatgcttct gtccgacaaa
      781 ctccagcaga cacaatccct tatttccgaa caccgccagc atctcgccca aaggcgtggc
      841 aatggcggca cacaccagct cgttcaagct gtcgggataa cgcacttcca acagacggat
      901 ggctcggcgg atacggacat attcttcagg cgcgcagccg gtattgtccc aaaaatcccg
      961 atcgaactgc ccggcttcgc attccgtcag attgggatgc ggcataacgc cgcactcaaa
     1021 aacccgggtt tcgaggcaat ggcggatttc atcccatttt gacggcagat tgtttaagga
     1081 aggcagggta atcattcgtt tgctccgtat ctctatcatg gatttggcgg caaaatcccc
     1141 aattctcgtc attcccgcac gccgatgcag gaacgggcta tgacgtaaat cttgagggtt
     1201 gggttgcggc aatgcctaaa tatgcgatat ttctaaagcc tcagggaaag gaattaaaga
     1261 cttcagacga cctgcaaagg gtcgtctgaa accacgattt ttgcatttac gcattccggc
     1321 acatcatcca accgtttcgg cacattcctg ccgccgttga cagcctataa tgaatccact
     1381 tattcatcaa gcaaaggaat catctatgca aacccgcatc ctctccgccg tactgctggc
     1441 tttttcaacc gctgcctttg ccgggggcgc attcacgctg caattcgaca acccgtccga
     1501 agacggcggc ttcacgcaaa accagctttt gagtgcgcct tacggcttcg gctgttcggg
     1561 cggcaatgct ccgcccgcgc tgtcgtggaa aaatccgccc gccgggacaa aaagtttcgt
     1621 cctgaccgtt tacgataaag acgcgccgac cggactgggc tggatgcact gggtggtcgc
     1681 cgacattccc gccgatgtcc gccgccgcaa cgcggcctcg ctgcaattaa gccgctgcgc
     1741 caacatcgcc gacgaccagt ccgcagccat atcggcggta atcagtttgc ggatttgccg
     1801 catcaggttg acgccttcgt acacggcaaa accgatgccg tcatgctgca accacgccaa
     1861 cacgccgcaa agcgcggcct ccgccacatt gtgcggcact ttttcatccg ccagcaccgc
     1921 agccgcataa tcaaacgccg cgcccatccg cccgggatgc ggcagcttca ccgcatcgca
     1981 cactgcctgc gccgtcccgt attgtgcggc aaacctttct atggcttcct gttcgaaagc
     2041 aatccattgc gcctgataga ggtcgtctga atcgggaata ttgatgacgt caaacgtctg
     2101 cccgcctgcc aaggcgaccg cctcacccgc cgcaatcctc aattcgcgcc tcgctcaccg
     2161 cagcttcttg cttccgcgcc gcacgataag cacagccggt tcatataccg ccacgctgcg
     2221 gtacaaggcg gtatgatgtt gcacgatgcc gcccaaagca cccaatcgtt cgcgcgtatg
     2281 aaagtaattt gacggcacta ttatctccaa cacgcagggc cgcacataag gcgcagccct
     2341 gtttaccctg atttggaagg ggttacttcc caaaaataaa ccaaaatccc actgccccga
     2401 atggccgggt ttcaaccata ttccgtcggt gtgcctgcaa cttgactccc tcccctgtgg
     2461 gggagggttg gggagagggt aaaacggggc agatacagac aatatttccg ttaccgcccc
     2521 gatgccctct ccctaaccct ctcccacggg agagggaatg gattgccgtt gaaataaatc
     2581 gctctacata aaaaatcaat gtgttatctc aaacccacgt taggtctaag caaatggtcg
     2641 gacatccgta tccggcaagc aagctgcttt cagacggcat ttccagccaa caagcgcgcc
     2701 aatatcccct catacaccgc cgacagcttc ggaatatcgt ccagccgcac gttttcgttg
     2761 atttggtgga tggtcgcatt ggacgggcct aattcgatga gttcttttgc aatggctttg
     2821 atgaagcgtc cgtccgaagt gccgccggtg gtggacaatt cggcctcaat gccgcaggtt
     2881 tcggcaatgg ctgcgcgtgc cacgtcggtc agtttgcccg cttgggtcag aaagggctgc
     2941 cccgaacacg accactgtaa atcgtattgc acgccgtgtt tgtccaaaat ggcgtgaacg
     3001 cgttgtttca gccctgcttc ggtggactcg gtggagaagc ggaaattgaa tttgacgctc
     3061 agctcgcccg gaatgacatt ggtcgcgcct gtaccgccgt tgatattgga aatttgaaag
     3121 ctggtcggcg ggaagtattt gttgccttcg tcccagactt cctgcgtcag ctctaacaag
     3181 gccggggcaa aagtatgcac gggattgatt gccaaatgcg gataggcaat atggccttgc
     3241 ttgcctttga cggtcaggtt gcccgacagc gagccgcgcc ggccgttttt aatcatatcg
     3301 cccaatttgt ccacggctgt cggttcgccg acgatgcagt agtcgataag ctcgtcgcgc
     3361 gctttcaaca catcgacgac tttggtcgtg ccgtccagcg cgtcgccctc ttcgtcggaa
     3421 gtaatcagga gcgcgatgct gccttgatga tcggggtgtt cggcaacaaa gcgttcgcag
     3481 gcggtaacga aacaggcgat gctggttttc atgtccgccg caccgcgccc gtataatctt
     3541 ccgtcgcgct cggccggctc gaacgggggc gaatcccatt tttcgacagg gcctgtcggt
     3601 acaacgtcgg tatgccctgc aaaacagacg acgggagctt tcgtgccgcg tcgcaaccag
     3661 atgtttttgg tgtcgccgaa atggagttct tcagccgcaa aaccgatttt gtgcaggcgt
     3721 tcggcaagga gtttttggca attttggtcg tcgggggtaa cggatgggcg ggaaatcaat
     3781 tctttggcga gttctaggga ttgggtttcg gtcatatttg ttcacttttg aaattagacc
     3841 atctgaaacg ttctgaatgt gattttcaga cggcatttag gttaggttgg catacggggt
     3901 gggtattttt acccgtcagt cttctgaatc atttgccgtg acacgcttcg taaagcggca
     3961 gcaaatcttc caccgtttcc gctatccatt tcgcgacatc ctgcctgccc aaatcgtcgc
     4021 gttcgatgtg tttgccgatg cagaagaaat cttcgtcgtt ttgcaacctt cggtcggatt
     4081 cattttgctg cgcgacagtg cggtagtcgt cgtattcgct ttccgcgccg tgccacatat
     4141 cgaaagaagc gtatttttca gtatcaaaat tatccaacca acggttgtaa tcgggcagag
     4201 taatcggcga cacgtcggct ttgtagcagt gccaatccaa gctgacactc agacggcggc
     4261 ggttaagtag tatcgacagg atggcggcag agtttttgta ttgggcgtat ttgaaatagg
     4321 caaagaaatg ggcgcgaacc tgccagccgt tacaccagcg ttcgatgtgc ggcggcgcaa
     4381 acggcgcacc caattcggcg gcaacctgct gaatcagctg ctgccatatc tgccagtttt
     4441 ccttgtagtc ggctttgatt tgcggaatgc tttcaggccg gtatttttta agctgggcaa
     4501 actggaaaaa cgggatattg aacaaatcgc aacttttcgg ggtcagcata atatatcctt
     4561 gagacgattg tttcagacgg cattatttgc gccggcgcgc cgccataatt tcgccgattt
     4621 cggtcagttt ttcttttggg ataaaggtgt tgcccatatc aaacagcggc tcttcaatcg
     4681 ccaaatgaac atcatatccc gccacaaaac gtttgaacgc ttcctcatcg gggacataag
     4741 cgttgtctgc ttcgagtttg gcaaattcgg cggaaacagc cgcccagttg tcgtgcagcc
     4801 cgatatgttg gcgcaaaagc tcgtccacgc tttcttgggc ttgcggcgca tattgcagca
     4861 gcagcgggaa gaagttttct tcttcgtctt catggtgcag cggcgcggca acgttgaaat
     4921 actgggcgat ttggcggatg gtttgcaaaa caatctgatt gcagccgttt tcggcgatat
     4981 agtccgacag catggcgact tgtccgcaaa aacggcgcac tttgccgtgg caggcataca
     5041 gcatttcaat cggttcggca aaggtaacgc ttttggtttc aaacggattc atgttttcgt
     5101 tctcaacggg cacttttcaa gcagtcattt tataataaaa cagcctgcac aaagcaggct
     5161 gtccgtcttt tgagacttta agcggattaa tcgaccaaag tcactttgcc gttcatcaaa
     5221 gcaccgtgac ctgggaaggt acaagcgaat ttgtattcgc cgtcagccag tttggcagga
     5281 tccaaagtca gggaagactc ttcgccgccg ccgatcagtt tggtgtgggc aacaacgcgc
     5341 gcatcgtcag gtttgacata gtcggtatcg gcagcaccta cgccgtcttt aaatacgccg
     5401 tccatgtctt cagctttggc aatcacaagg ttgtgaccca tgctggcttt gggttgcgta
     5461 ccggtatgtt tcagagtgat ggtgaactct ttacatgctt tgctgacttg gatgtctttg
     5521 gtgttgaact gcatattgtc gttggattcg acagttgccg cacaattgcc ggcagcaggg
     5581 gcttcggcag catctgcagg agcagcttcg gcggcaggcg cttcggaagc gggtgcttca
     5641 gcagcaggag ttgcctcggc agcaggcgcg gcaggttctt gagagcaggc agccaaaccg
     5701 ataacggcgg cagaaatcag agccagatac gctttcataa caaatctcca atcgataaaa
     5761 taatattcgg ttttacagaa atcaaagtgc aaccgccatt aacaaaacct tgaaaaagat
     5821 tccgccgcgt tgcacaaaca gatgtttcgg ggcggcattt tgctacaaat ttcatttgaa
     5881 atcaaagcct gtctgcaagt ttacaatcgt ttactttaaa aggggcaatt ttaccccgaa
     5941 cctatttctt tagtattaga cccattatcc tttacttctt aatattaacg gatgtttaca
     6001 caaattcccg tatacatttt atgcgccatg ccttctaacc aagtttgcca atgcctccgc
     6061 caattcggga tgccgttttt ccaactttac cgccgccgaa ccgaaactct ccagcgcagc
     6121 cttactcaaa tgcagggtat tggttttcgg cggtttttcc ggtttcgggg ccagcctgac
     6181 cgaaacagag cgtatcgaag catcaagccc tgccaactgc ggcaataccg acggtgcaat
     6241 cattttcaag cgcgatgccg ccatattgtt tgccgccaaa aggacaagcc tgccgtcttc
     6301 gatacatgcc gtctgaaaat gcgggtgcag gttggcaggc agcagttttt tcacggcggc
     6361 atccaaccgc cgccactgtc ccgcctgttt caaaagtccg gaaagcagcg cgtcccgcct
     6421 gcccaactgt tccaaattca taaaacatac acccaaaaag attgaaatac cgcaaacgcg
     6481 cctttatttc agacggcatt agcactttgc acaaacgctt gtgttaaaat cgcgttttcg
     6541 cccactatta tatcaggcgc aggaattatt catgctgaca aacattgcca agaaaatctt
     6601 cggcagccgc aacgaccgct tgctgaaaca ataccgtaaa tccgttgcca gaatcaacgc
     6661 gctcgaagaa cagatgcaag ccctaagcga tgctgatctg caagccaaaa ctgccgaatt
     6721 caaacaacgc ctcgccgacg gtcagacttt ggacggcatt ttgcccgaag ccttcgccgt
     6781 ctgccgcgaa gcgtcccgcc gcaccctcgg tatgcgccac ttcgacgtgc agcttatcgg
     6841 cggtatggtg ctgcacgacg gcaaaatcgc cgaaatgcgt accggcgaag gcaaaaccct
     6901 ggtcgccacc ctcgccgtct atctcaacgc gctggccggc aaaggcgtac acgtcgttac
     6961 cgtcaacgac tacctcgcct cacgcgatgc gggcattatg gagccgctct ataatttcct
     7021 cggtctgacc gtcggcgtga ttatttcaga tatgcagccg ttcgaccgtc aaaacgccta
     7081 tgccgccgat atcacctacg gcaccaataa tgaattcggc ttcgactacc tgcgcgacaa
     7141 tatggttacc gaccaatacg acaaagtgca gcgcgaattg aattttgccg ttgtcgatga
     7201 agtggattcc atcttgattg acgaagcgcg cactccgctg attatctccg gtcaggcgga
     7261 tgacaacatc cagttgtacc aaatcatgaa caccgttccg ccccacctcg tccgtcaaga
     7321 gacagaagaa ggcgaaggcg actattgggt cgacgaaaag gcacatcagg tcatcctgag
     7381 cgaagcaggt cacgaacacg ccgagcaaat cctgacccaa atgggattgc tggcagaaaa
     7441 cgactccctc tattccgccg ccaatatcgc cctgatgcac caccttatgg cggcattgcg
     7501 cgcgcattcc ctcttccaca aagaccaaca ttacgtcatc caagacggcg aaatcgtcat
     7561 cgtggacgaa ttcaccggcc ggctgatgtc cggccgccgc tggtcggagg gtctgcatca
     7621 agccgtcgaa gccaaagaag gcgtggaaat caaacgcgaa aaccaaaccc ttgcctccat
     7681 caccttccaa aactatttcc gcctgtacac caagctctcc ggcatgaccg gcacagccga
     7741 taccgaagct ttcgagttcc aaagcatcta caacctcgaa accgtcatca tcccgaccaa
     7801 ccgccctgta cagcgcaaag acttcaacga ccagattttc cgttccgccg aagaaaaatt
     7861 cgaagccgtc gtcaaagaca tcgaagaatg ccacaaacgt gggcagcccg tcctcgtcgg
     7921 caccaccagc atcgaaaact ccgaactggt ctcccacctt ctgcaaaaag ccggactgcc
     7981 gcacaacgtc ctcaacgcta aagaacacga acgcgaagcc ctgattgtcg cccaagccgg
     8041 caaagtcggc gcgattaccg ttgccaccaa tatggcggga cgcggtacgg acatcgtttt
     8101 aggcggcaac ctgaagcacc aaaccgatgc catccgtgcc gacgaaacct tgagcgacga
     8161 agagaaacag gcacaaatcg ccgcactcga aaacggctgg caggcagaac acgataaagt
     8221 aatggaagca ggcggcctgc acatcatcgg tacggaacgc cacgaaagcc gccgtatcga
     8281 caaccaattg cgcggacgtt ccggccgtca gggcgacccc ggatccagcc gtttctacct
     8341 ttcgtttgaa gacccgctgc tgcgcttatt cgcactcgac cgcgccgccg ccatcctcaa
     8401 ccgtctcgcc cccgaacgcg gcgttgccat cgaacacaac ctgctgacgc gccaaatcga
     8461 aggggcgcaa cgcaaagtcg aaggcagaaa cttcgatatg cgcaaacagg ttttggaata
     8521 cgacgacgtt gccaacgaac agcgcaaagt catttacagc cagcgcaacg aaattctgac
     8581 cagcaaagac atcagcgacc tgatgcagga aatccgttct gatgtcgtca gcgacctcgt
     8641 ggatacctat atgcctcccg acagcatgga agaacaatgg gacatcccga ctttggagaa
     8701 ccgtctggct gccgaattca gactgcacga agacatccaa tcctggctga aggcggacaa
     8761 tgcgattgac ggtcaagaca tcaaagaacg cctgatcgaa cgcatcgaaa acgaatatgc
     8821 cgccaaaacc gaactggtcg gtaagcaggc aatggccgat ttcgagcgca acgtgatgtt
     8881 gcaggtcatc gacaaccaat ggcgcgaaca cctcgccgct atggactacc tgcgacaagg
     8941 catacacctg cgcagctatg cccaaaaaaa tccgaagcag gaatataaac gtgaagcctt
     9001 taccatgttc caagacctgt ggaacggcat caaattccat attgcctccc tgcttacctc
     9061 ggttcaaatc gaacaaaacc ctgtcgcggt ggttgaagag caacccatcg gcaacatcca
     9121 gtccatccat tccgaatcgc ccgatatgga agaacttttg ggtcagtcgc aaaccgatct
     9181 ggttaccgaa gcctttaatc ccgatgggac agatttcagc cccgaagcct tggaagcgcg
     9241 ggggcaaatc gtccaccgca acgacccctg cccctgcggc agcggtttga aatacaaaca
     9301 atgccacggc aaactggctt aagcgtttga acgcaaatgc cgtctgaaca tcccgctccc
     9361 gtttcagacg gcattttgcc tgaaccgcca catccgactg ccattccgaa aaatcccgat
     9421 ttcgtaccgt ccgtaccaaa aacagacatc ccgtccgccc cacatcatga ttccatccga
     9481 cttcattgac gagcttttag ccaaaaccga tattgtcgat attatcgacg agcaggttcc
     9541 gctgaaaaaa ggcggggcga actatatggc gtgttgcccg ttccacaagg aaaaaacgcc
     9601 gtcgttttcg gtcagtccaa ccaagcagtt ttaccattgt ttcagttgcg gggcgcatgg
     9661 ttcggcgatt ggttttgtga tggagcatca ggggctgtcg tttccggagg ctgtgcagtt
     9721 tcttgccgac cgtgtgggca tggttgtgcc taaagtgcgt gggcagaacg ataatcccga
     9781 agtccgtgcc gaacgtaaga aaaaacagca gacgctggaa gaaacgacgg cggcggcggc
     9841 tgatttttac gcgcaacagc taaaattcaa tccggcggca aaagcttatt tggacaagcg
     9901 cggcttgagt gcagaagtta tcgcgcatta tggtttgggc tacgcgcccg acggctggca
     9961 gcctttggcg caagtgttcc aaccgtatcc gaataccgcg ttggtggata cggggatggt
    10021 gattgacaat gaggggcggt attacgaccg cttccgccat cggattatgt tccctatccg
    10081 caatccgcgc gggcaggtca tcggtttcgg cggcagggtg ctggacgact ccaaacccaa
    10141 atatctaaac tctcccgata cacctttgtt tgacaaaggg aaaaaccttt acggcttgta
    10201 tgaggggcgt gccgctgtca aggaagcgga gcggattctg gtggtcgaag gctatatgga
    10261 cgtggtcgcg ctggcacagt tcggcgtggg ctacggtgtg gcggccttgg gcacggcaac
    10321 aacagctgaa cacgtcaaaa tcctaatgcg tcaggcagac agtatttatt tctgtttcga
    10381 cggcgacagc gcggggcgaa aagcggcttg gcgcgcgctg gaaaacgcgt tgccgcagtt
    10441 gaaagacgac aaatcgctgc attttttgtt cctgcccgag gaacacgacc ccgacagtta
    10501 catccgtgcc tacggcaaag cgcaatttga agacgcgctt ctgaatcaaa gcaagccttt
    10561 gtcggagtat ttctgggaac acctttcaga cggcattcat ctcaatacgc aggaaggcaa
    10621 ggcggaattg gtgaaaacca gttcgccgct tttggcgcag attaccgcgc cggcattggc
    10681 ttatttgtta aaacaacggc ttagcgaact ggtcggcatc gaccccgaca acctcgcgca
    10741 actgctagga caggaagcgc cgaagcggca cgtcaaacaa aaaaactaca aactgcctcc
    10801 gatttccgtc aaacagcccg tcatgctgac gctggtacag cggcaaatcc gcagcctctt
    10861 gataaatccg gattgggctg catatataga cctgcccgat tatctggcgt tggacggtga
    10921 tttcgcctgc cttgccaatc ttgccgaatc gattaaaaac catgccgccg tacccgaaac
    10981 cgctcaggtt ttagagtata tgcgcggctc gccttacgaa gaaacgataa cccgaatctt
    11041 ccattcaacg caccaatcgg aagaaatgaa cagcagcagt gaagaagatt gcgagaattt
    11101 ccaaatcggc atgaaaaaac tgctcaatga gttaaaatac agccaaatcg aagcattgaa
    11161 acaaaaaagc ctgcaatccg gcttaaatga aagcgagaaa aaacttttgc tgtcgttgct
    11221 gaccgcaaaa caaaattgac cggcggattc cgccacccgt aaacccgtta tgccgtctga
    11281 aaagcactta ccccggctgc aacaacgaca cctgcagaac acccatcccc aaaagccttc
    11341 agacggcatc agagtaccct actctgccac gccttcaggt gcgtccaaac gcaaaccgtc
    11401 ggcatcttgc caacagaaag cagacaatgt ccaaaaacca aaatcacgaa gaataccaag
    11461 acgatgcccg acccctgacg attgaggaac aacgcgcgcg cctgcgccaa ctcatcatta
    11521 tgggcaaaga acgcggctac atcacctact ccgaaatcaa tgacgcactg cccgacgata
    11581 tgtccgatgc cgatcaaatc gacaacatcg tcagtatgat ttccggtttg ggtatccaag
    11641 ttaccgaaca cgcccccgat gcggaagaca tattgttaag cgacaatgcc gccgttaccg
    11701 acgatgatgc cgtcgaagaa gccgaggccg ccctttccag cgccgactcc gagttcggca
    11761 ggacgaccga ccccgtccgt atgtatatgc gcgaaatggg acaggtcgac ctgctgaccc
    11821 gcgaagacga aatcatcatc gccaaaaaaa tcgaaaacgc cctgaaaaat atggttcagg
    11881 ccatctccgc ctgcccggga tccattgcgg aaatcttaga actcatcgaa aaaatccgca
    11941 aagacgaaat ccgcgtcgac gaagtcgtag aagccattat cgacccgaat gaagtattgc
    12001 tcaacgaatt gggcttgggg cacttggaaa ccacagcgcc cgagaaacct tccaacgaca
    12061 attcggacga aaacgaagac gacgaagaat cggaagaaga tgcggatgaa atctcggcag
    12121 ccaatctcgc cgaattgaaa caaaaagtca tcggccactt tgcccaaatc gaaaaagact
    12181 acaaaaaaat gatcggtcgt ttggaaaaac accacagccg gcacaaagac tatctcgcct
    12241 accgcgacgc gattgccaac aaactgctgg aagtccgttt cgccacccgg caaatcgaca
    12301 gcctcagcag cagcctgcgc ggaaaagtag aaaacatccg caaactcgaa cgcgaaatcc
    12361 gcgacatctg cctcgaccgc gtccatatgg agcgcgacta cttcatccaa aacttcctgc
    12421 ccgaaatcac caatctggaa tggattgaag aagaaatcgc caaaggcagg gtttggagcg
    12481 acgcgctcga ccgcttccgc cacgccatcc tcgaaaaaca aaccgagttg gcagatatgg
    12541 aaaaagaaac ccgcatttcc atcgaagagt tgaaagaaat caacaaaaat atggtgtcga
    12601 gcgaaaaaga aaccgcagcc gccaaacagg aaatgattca ggcaaacttg cgcctcgtca
    12661 tttccattgc caaaaaatac accaaccgtg gcttgcagtt ccttgatttg attcaggaag
    12721 gtaacatcgg cctgatgaaa gcggtcgaca agttcgaata ccgccggggc tacaaattct
    12781 ccacctacgc aacctggtgg atccgccagg caatcacccg ttcgattgcc gatcaggcgc
    12841 gtaccatccg cattccggta catatgattg aaaccatcaa caagatgaac cgcatctcgc
    12901 gccaacacct tcaagaaacc ggcgaagaac ccgattccgc caaacttgcc gaactgatgc
    12961 agatgcccga agacaaaatc cgcaaaatca tgaaaatcgc caaagagccg atttcgatgg
    13021 aaacccccat cggcgacgac gacgattcgc acttgggcga cttcatcgaa gatgccaaca
    13081 atgttgcgcc ggccgatgcg gcaatgtaca ccagcctgca cgaagtaacc aaagaaatcc
    13141 tcgaaagcct gacaccgcgt gaggcaaaag tcctgcgtat gcgtttcggc atcgatatga
    13201 acaccgacca cacgctggaa gaagtcggca gacagtttga cgtaacgcgc gaacgcatcc
    13261 gacaaatcga ggcaaaagca ctccgcaagc tgcggcatcc gacaagaagc gaccgtttga
    13321 gaagtttctt ggacagcgaa gacagcaagc tgtaaaccaa aaaaccgcag gtttcaaata
    13381 cctgcggttt tttcttacac aataaacaac gcttccacat atcccacact cctatcccga
    13441 gacctttgca aaatccccca aaatccccta aattcccacc aagacattta ggagcacctt
    13501 cttccaacaa accgcccaag ccatgattgc caaacacatc gaccgtttcc cgctattgaa
    13561 gttggacc
//
LOCUS       DAWKST010000045        13482 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.045, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000045 DAWKST010000000
VERSION     DAWKST010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13482
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.045"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(144..234)
                     /locus_tag="WJL87_000897"
     tRNA            complement(144..234)
                     /locus_tag="WJL87_000897"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(198..200),aa:Ser,seq:gga)
     gene            complement(273..359)
                     /locus_tag="WJL87_000898"
     tRNA            complement(273..359)
                     /locus_tag="WJL87_000898"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(323..325),aa:Leu,seq:cag)
     gene            complement(395..481)
                     /locus_tag="WJL87_000899"
     tRNA            complement(395..481)
                     /locus_tag="WJL87_000899"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Leu,seq:cag)
     gene            570..2945
                     /gene="rnr"
                     /locus_tag="WJL87_000900"
     CDS             570..2945
                     /gene="rnr"
                     /locus_tag="WJL87_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEZ7799343.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGIDRRGRREAVVLDIVERAQ
                     SKVVGRFYMDRGVAVLEAEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAAKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPNVERLCMVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSGGIGHPFKAQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLAALREQL
                     GLLGLQLGGGDNPSPKDYAALAGQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDGIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAEGSGRGRKAKSSASVKPAG
                     TAGKGKPKTAAEKKTARGGKVRGRGASAAAESGKKAKKPVPIKVKKRKGKS"
     gene            complement(3284..4747)
                     /gene="guaB"
                     /locus_tag="WJL87_000901"
     CDS             complement(3284..4747)
                     /gene="guaB"
                     /locus_tag="WJL87_000901"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ7799344.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVKWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(4948..5028)
                     /locus_tag="WJL87_000902"
                     /pseudo
     CDS             complement(4948..5028)
                     /locus_tag="WJL87_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(5074..5535)
                     /locus_tag="WJL87_000903"
     CDS             complement(5074..5535)
                     /locus_tag="WJL87_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799345.1"
                     /translation="MNIIMDSTRKFGILWEENSECNGFIYGKIQIIIGENIYPKICPY
                     GYFTLNAVFNSLKSSFEEKYYAGGNNGLDFGEQLFDIDKYNSLELCNIFSIDTTYMSG
                     GSNCEIDCLVLEMGYSGEEERLFYSFDNGKNFKEIRYKKGTVESVIFQLNL"
     gene            complement(5626..8184)
                     /gene="glnD"
                     /locus_tag="WJL87_000904"
     CDS             complement(5626..8184)
                     /gene="glnD"
                     /locus_tag="WJL87_000904"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEZ7799346.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWAEYFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGSVDELCESVRNDITGDTAFLEARFLFGNRQTVDKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNANRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRHRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILTVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTGEESDLLAWLVENHLLMSAVAQKEDIQDPDVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPQSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAEISSHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEIFFAHNVSLRYAKISTLDERAEDSFTVFSPDLKNPKIQSSLKQTLLEQLS"
     gene            complement(8233..8553)
                     /locus_tag="WJL87_000905"
     CDS             complement(8233..8553)
                     /locus_tag="WJL87_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7799347.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLKLMTNSADTRQMPS
                     ESGI"
     gene            complement(8878..9351)
                     /gene="bfr"
                     /locus_tag="WJL87_000906"
     CDS             complement(8878..9351)
                     /gene="bfr"
                     /locus_tag="WJL87_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ7799348.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(9379..9843)
                     /gene="bfr"
                     /locus_tag="WJL87_000907"
     CDS             complement(9379..9843)
                     /gene="bfr"
                     /locus_tag="WJL87_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ7799349.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(10306..11289)
                     /gene="lipA"
                     /locus_tag="WJL87_000908"
     CDS             complement(10306..11289)
                     /gene="lipA"
                     /locus_tag="WJL87_000908"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ7799350.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(11282..11905)
                     /gene="lipB"
                     /locus_tag="WJL87_000909"
     CDS             complement(11282..11905)
                     /gene="lipB"
                     /locus_tag="WJL87_000909"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ7799351.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNANRTASTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPIVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKGGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(11907..12182)
                     /locus_tag="WJL87_000910"
     CDS             complement(11907..12182)
                     /locus_tag="WJL87_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ7799352.1"
                     /translation="MTEQKNKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHKLVKVVL"
     gene            12367..13437
                     /locus_tag="WJL87_000911"
     CDS             12367..13437
                     /locus_tag="WJL87_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ7799353.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LAVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
BASE COUNT         2924 a         3189 c         3823 g         3546 t
ORIGIN      
        1 taccgcaaaa cataccataa aaaaaacggt aacggatagt tgtaagcggt tgatgacgat
       61 tatagacaat aacgggtttt cccaatgaaa ttattgtttg aataataaaa aatcccaaac
      121 cgtaaaagtt tgggatttgt atttggcaga gaggaaggga ttcgaaccct cgatacgcta
      181 ttcacgtata cacgctttcc aggcgtgcga cttaaaccac tcatccacct ctctaatggc
      241 ggaaattatc ccaatcggga taatttatta tttggtgcct gggagaagac tcgaacttcc
      301 acacccgtga ggataccagc acctgaagct ggcgcgtcta ccaattccgc cacccgggca
      361 atctaaatat tgaaataaag caaagcattt gatttggtgc ccgggagaag actcgaactt
      421 ccacacccgt gaggatacta gcacctgaag ctagcgcgtc taccaattcc gccacccggg
      481 ctttgcttta tttgctgttt tgcggtacag tgtcctgcag caaagaagtc gctattatat
      541 agttcataaa gagaatgtca acagtccaaa tgaataaaaa tattaaatct ttaaatttac
      601 gggaaaaaga cccgttttta agtcgtgaaa aacagcgtta tgaacatcct ttgcccagtc
      661 gggaatggat aatcgaattg ttggagcgca aaggtgtgcc ttcaaaaatc gaatcgcttg
      721 cgcgcgagct gtcgattacg gaagacgagt atgtcttttt cgaacgccgt ctgaaggcga
      781 tggcgcggga cggtcaggtt ttaatcaacc gccggggcgc ggtttgcgcg gcggacaaat
      841 tggatttggt caaatgccgt gtcgaggcgc acaaagacgg cttcggtttc gccgtgccgc
      901 tcacgcccgc caaagacggt gattttgttt tgtacgaacg ccagatgcgc ggcattatgc
      961 acggcgatat tgtcactgtt cgtcctgccg gcatcgaccg cagggggcgg cgggaagcgg
     1021 tggttttgga tattgtcgaa cgcgcgcaaa gcaaagtggt cgggcgtttc tatatggatc
     1081 gcggcgtggc ggttttggaa gcggaagaca agcgtctgaa ccaaagcatc gtattggaac
     1141 cggacggcgt ggcgcgtttc aaacctgaat ccggtcaggt catcgtcggc gaaattgagg
     1201 tttatcctga gcaaaaccgg ccggcagtgg caaaaatcat cgaagttttg ggcgattatg
     1261 ccgacagcgg catggagatt gaaattgccg tgcgcaagca tcatttgccg caccaattca
     1321 gtgaagcgtg tgccaaagcc gcgaaaaaaa ttcccgacca tgtacgcaaa agcgatttga
     1381 aaggccgcgt cgatttgcgc gatttgcctt tggtaacgat agacggcgaa acggcgcgcg
     1441 attttgacga tgccgtgttt gccgagaaaa tcggacgcaa ttaccgtttg gtcgtggcga
     1501 ttgccgatgt cagccattat gtccgccccg atgacgctat cgacacggac gctcaggaac
     1561 gcagcaccag tgtttacttc ccgcgccgcg tgattcccat gttgccggaa aacctgtcca
     1621 acggcatctg ctcgctcaat cctaatgtcg agcgtttgtg tatggtgtgc gatatggtca
     1681 tcacttacgc gggcaatatt aaagaatacc gcttctaccc cgccgtgatg cgctctcatg
     1741 cccgcctgac ctacaaccaa gtttggaaat ggctttcagg cggaatcggg catccgttca
     1801 aagcccaaat cgacacgctt tacaaactct tcaaaatcct tcagaaaaag cgtttcgaac
     1861 gcggggcggt ggagtttgac agcatcgaaa cccaaatgct tttcgacgac aacggtaaaa
     1921 ttgaaaaaat cgtccccgtt gtccgcaacg atgcccacaa gctgattgaa gaatgtatgt
     1981 tggcggcaaa cgtttgcgca gcggattttc tgttgaaaaa caagcatacc gcattgttcc
     2041 gcaaccattt ggggcccacg cccgaaaaac tcgccgcctt gcgcgagcag ctcggtctgt
     2101 tggggcttca acttggcggc ggcgacaacc cgtcgccgaa agactatgcc gcgcttgccg
     2161 gacagttcaa aggcaggccg gatgccgaat tgctgcaagt catgatgttg cgctccatgc
     2221 aacaggcggt ttacgaaccg cattgcgacg gacactttgg tcttgcctac gaagcatacg
     2281 cccacttcac ctcgcccatc cgccgctatc ccgacctgac cgtacaccgc gccatcaaag
     2341 ccgtgttgaa tcagcaaacc tacacgccaa aaaaaagctg gcaggctttg ggcgtgcata
     2401 cctcgttctg tgagcgccgt gccgacgacg ccagccgcga cgtggaaaac tggctgaaaa
     2461 cctattatat gcgcgataag gtcggcgaag tattcgaagg taaaatctcc ggcatgacca
     2521 gttttggtat ctttgtaaca ctggacggca tccacattga cggcttggtg catatcagcg
     2581 atttgggcga agactatttc aacttccgcc ccgaaatcat ggcaatcgaa ggcgaacgca
     2641 gcggcatccg tttcaacatg ggggacaggg ttgccgtccg ggtcgcccgt gccgatttgg
     2701 atgatggaaa aatcgatttt gtcctgattg ccgaggggag cggcaggggg cggaaagcta
     2761 aatcatccgc gtctgtcaaa ccggcaggga cggcggggaa agggaaaccg aaaaccgccg
     2821 ccgagaaaaa aacagcccga ggcggcaaag taaggggaag gggcgcgtct gccgccgcag
     2881 aatcggggaa aaaggcaaag aaaccggttc cgattaaggt aaaaaaacgg aaaggcaaat
     2941 cataatgctg acggggtggc ttggggaggg cggggcatac tttcccgaag ccgccgcccg
     3001 attttttgat atggcggccg gcgcatcatt tcaggccaat cgaaacgccc gtattgaaag
     3061 attgcgttta tttccaccgc cgttttgaag gccggcggta ttcggcagac ggggcgcaaa
     3121 cggcgttcag acggcatttt cattctttcg gcgtgtccgt ccgaattgct ttgcccgtcc
     3181 gcgcaatcag cccgtgcggc ttgccttgaa cggacaaaaa atgccgtctg aaacccgaaa
     3241 atcaggtttc agacggcatt ttgccttgaa aaggctgttc aaatcagcgg tggtagttcg
     3301 gtgcttcttt ggtaatttga acgtcgtgaa cgtgcgattc gctcatacct gcggaagtga
     3361 tttccacaaa ttctgctttt tcgtgcattt cggcaatatt ggcgcaaccc aaatacccca
     3421 tgctggagcg cagtccgccg gtcagttggt ggatgatgtt cacaatcggg cctttgtagg
     3481 gaacgcggcc ttcgatgcct tcggggacgt atttgtcggt gctgtcggtt ttgtcttgga
     3541 agtagcggtc ggcagaacct tggctcatcg cgcccaatga acccataccg cgataggatt
     3601 tgtacgagcg gccttggtag agttcgattt cgcccggcgc ttcttccgtg cctgcaaaca
     3661 taccgccgag catgacgctg tacgcgcctg cggcgagggc tttggcgatg tcgccggaga
     3721 agcggatgcc gccgtcggca atcagcggaa cgcccgtgcc tttgagggct tcggcaacgt
     3781 tgtgaatggc ggtcagttgc ggcacgccga cacctgccac gatacgggtg gtgcaaatcg
     3841 atcccggacc gataccgact ttgacggcat ccgcgccggc ggcgaccaaa tccaatgcgg
     3901 ctttggcagt ggcgatgttg ccgccgatga cttggatgtg cggataggtt tctttcaccc
     3961 atttcacgcg gtcgatcacg ccttggctgt gcccgtgggc ggtatcgacg acgatgacat
     4021 cgactcctgc ttctaccaat gcttttacgc gctcttcggt gtcgccgccg gtgccgactg
     4081 ccgcaccgac gcgcagacgg ccctcggagt ctttgttggc attgggaaat tcggtggttt
     4141 ttaaaatatc tttgacggta atcagacctt tgagttcgtc tttttcgttc agaaccaaaa
     4201 cgcgctcgac tttgtgcgtg tgcatcagtt cgcgcgcttc gtctatgctt gtgccttcgg
     4261 ggacggtaac cagacgttcg cgcggggtca taatggcgga aacgggcaaa tcgacgcggt
     4321 tttcaaaacg caggtcgcgg ttggttacga tgccgacgac tttgccgttt tcaacgacgg
     4381 gcaggccgga cattttgcgt ttgcgctgcg cgcgcatttc caagacttcg cggatgagcg
     4441 tcgtcggcgc aacggttacg gggtctttga ccacgccgct ttcgtggcgt ttcactttgg
     4501 aaacggcgcg cgcctgcatc tccggcggca tatttttatg gatgatgccg atgccgcctt
     4561 cctgtgccat cgaaatggcg aggcgcgcct cggtaacagt atccatcgcg gcggaaagca
     4621 gggggaggtt gagcgtgatt tcgcgggtga gcttggtttg aagtttaacg tctcgcggca
     4681 gcacggtcga atgtgcggga accaacaaaa catcgtcgaa agtataggct ttttctacga
     4741 tacgcataat gctcggtctt tcagtttgtg caagatgcac ggcattatag cacgttaccg
     4801 gcggcttgac agtttatcag gtttaatttt ggtccccttt gaatagcccg gttttccttt
     4861 gccgaccact gttgctcccg ttctttcaat ttcaggaaaa gcttttttct aatttttggt
     4921 aagtggctca gttattgaag ccctatatcg ggcggtaact tccagcacaa aaaaacggag
     4981 tagttcttta tttatttttt cctttaattt tcagtatatt atcttaatat ttcgagggta
     5041 acatatctgc taatctagtt acagccccat atattataga ttcaattgaa aaataacaga
     5101 ttcaactgta cctttcttat acctgatttc tttaaagttt ttcccattgt caaaactata
     5161 aaaaagtctc tcttcctcac cagaatatcc catttctaat actaaacaat ctatttcaca
     5221 attactccct ccactcatat atgtagtatc aatagaaaat atattgcata attccagaga
     5281 attatattta tctatatcaa atagttgctc tccaaaatct aaaccattgt ttccacctgc
     5341 ataatacttc tcttcaaagg atgacttcaa actattaaaa actgcattta gcgtaaaata
     5401 tccatatggg catatcttag gatatatatt ctctcctatt attatctgaa tttttccata
     5461 aataaaacca ttacattcag agttttcttc ccataaaatc ccaaattttc ttgttgagtc
     5521 cataataata ttcatataaa tccttatatt aataaattat ttacaatatc ccccgctttc
     5581 agacggcata cggcgtggcg gcggaatgcc gtctgaaggc gggcgttatg ataattgttc
     5641 cagcagcgtc tgcttcaatg aggactggat ttttgggttt ttcaggtcgg ggctaaaaac
     5701 ggtaaaactg tcttcggcgc gttcatccag tgtggagatt ttggcatagc gcaggctgac
     5761 gttgtgggcg aaaaagattt ccgccatatc ggcgagcagg aaggggcggt tgacggcggt
     5821 aatttcgacg gagtaccagt cggggtagtc ttcttcgggg gtaatggtga tgcttggcgc
     5881 gatcggcatg tagcggctgc ggcggctgat gcggcggcta tggctggaga tttcggcaac
     5941 ggtgtgtccg tggataaagc tgttgagttc ggcttcgagc gcgctttgga tgtcggggta
     6001 gtcttcggga gcgtgctgcg aggggatttg cacgatgaag gtgtcgagga tgtagtcgtg
     6061 ttcggtaata aaggcgcggg cggcgagaat gtcgaagccg tggcggctga agatacggca
     6121 gaggcgggcg aacaggcgcg ggccgttggg cataaaaacc atgacttgaa agctgtcgct
     6181 ttggggcagg atgcggctgc ggacgatggg ggtttcaaaa tcgtgtacga ggttggcggc
     6241 gtgccacagg atttcgcggg actggtggcg ggcgaagtag gcggaaccga gcgcgttcca
     6301 tagttttttc tgctgttttt cggggacggc ggcgcgggtg agtaagtcgg cggcttcctg
     6361 ccggcggcgg ccgaagaggg tgtgcgggtt gccgccgttg cctgtaaggt agcgtccggc
     6421 ggcatggaag aggctttcca gcaggctggc gcgccatgcg ttccacagct tgggattggt
     6481 gccgcgtatg tcggaaatgg tcagaaggta gagcgcgctg aggcgttcgt gggtttggac
     6541 gcgtttgcag aaagcatcga gtacgtcggg gtcttggatg tcttcttttt gggcgacggc
     6601 agacatcagc aggtggtttt cgaccagcca ggcgagcagg tcgctttctt ctccggtcag
     6661 gaagtggtcg gcggcaaatt ggcgcgcgtc tgcgatgcct tgtatggcat ggtcgccgcc
     6721 gcgtcctttg gcgatgtcat ggaaaaaggc ggcaaggtag aggatgtctt gtttttcaaa
     6781 ggactgcatc agtgcagagg cgtagggcag ctcgtggctg tgcatatcca aggcaaggcg
     6841 gcggacgttg cggacgacgg tgaggatgtg gtcgtccacg ggatagatgt ggaacaggtc
     6901 gtgctggagc aggccgacga ttttttccca cgcgggcagg tagcgtccca atacgccgta
     6961 gaggttgagg aagcgcaggg tttgggtcag tccgttgccg ttgcggaaaa agccggcgaa
     7021 gcggtggcgg ttttcagaat tttggtagaa gctgcggttg attttgcgcg tcgcgcccca
     7081 ccatgcgcgc agggtttgcg gttcgagcgc ggtaatatcg ttgcgctgct gcatgatttc
     7141 gacgattttg aaaatgtgtt cgggccgtct gaaaaaaata tcggtgtgcc gcgcggcgat
     7201 ttggttgttg acttggatgt agtcgtcgtc aatccgcagg gtaacgcgca acggggtgga
     7261 ggaaacgcgg ctttgcagca taggcgtgag gatgccgccc agttgtttga cggttttaat
     7321 cgcgcggtaa aacacgcgca tcagttcttc gctttggcgg cggaggttca agccttcata
     7381 acccatgctt tcggcgactt gcggctgcaa atcgaacagc aggcggtctt cggcgcgatt
     7441 ggcgtttaaa tgcagatgga tgcggatgtg ggcgaggcgg cggtagccgt gcgaaagcat
     7501 accggcttcg gcacgcgtca aaatccgctg tttgagcagg tcgggcaggt cggtcgccaa
     7561 gccttgcgcc ttcgctatcc aaagcagggt gtggatgtcg cgcagaccgc ccggacagct
     7621 tttgatattc ggctccaata ctgcccccga accttgcgat ttggcgtggc ggtgttccat
     7681 ctccaccagt tttgcctcga caaacgccgc cacattgcgc tgcgcgttca ttttttccgc
     7741 cagtttatcc accgtttggc ggttgccaaa caaaaaccta gcctctaaaa acgctgtgtc
     7801 ccccgtaata tcattgcgca cgctttcaca tagttcatca acgctgccgc tttttacaga
     7861 cggcatcagt ttgcagtccc acagggtttg gacaaaccgg gcaatctgtt cctgaatgcc
     7921 gtctgaaagc ggggcagggg agacaaccgc caaatccacg tccgaacagg gatacagttc
     7981 gccgcgcccg aagccgccta ccgccatcag gcataacgcg ctgttttgaa aatactctgc
     8041 ccacaatgcc gccagtaagg tttcgactgc cgccgtgtat tctctgaaaa ataccgacac
     8101 gcggttggct ttcaaataat gcgcttcggc ggcatcgcgc tgctgtttga aggtttccag
     8161 tgctgaagac aggtttgcag gcatttttat tctttcgatt ggcgggaaaa agggaggcgg
     8221 atggttcggc ggtcaaatac cgctttcaga cggcatttgt cgggtatcgg cggaattggt
     8281 catcagcttc aaccgttcct gcggcggcag cagcaactga tacgccgcca cgcccaaagc
     8341 cgccgccatt ttttcgatat tcgacagggc gatgttccag cgtttgcgct cgactgccga
     8401 cacataagtc ctgtccaaac cgcattgccg cgccaattct tcttgcgacc aacccttgtt
     8461 cacgcggaaa agccgcatat tgtatgccaa taccgcccgc aaatcctgtt cgtcaggcag
     8521 ttcggcaggc agagtcaatt tgttgcccat catttgcttc caaataaatg gttaaagtta
     8581 agcatttgct gttacggatt ttacttcaca taaaagccaa aaaaaagggg tggcggcagc
     8641 cacacccaaa cacacacaca tcaagaggaa agaggtaaca tcaagacaga ttgggaaaca
     8701 gcaaacaagc aatttagatt tcatatcggt attatcaaac aaaaaccgtt atcgagtatt
     8761 gattcaaatc aattgagatt tgattcgcaa accggtaaag aaaaacggca tacccgttta
     8821 tacggatatg ccgtctgatg atgcagggga ctgctgtatt tggcagtggt ttgtgtttta
     8881 gtcctcttgc gccgctgttt gcaggtagtt cggcaaacca attttgccga tcagttcctg
     8941 ctgggtttca agccagtcga tgtgttcttc gttggtgtct ttttgttttt ccaacaaatc
     9001 acggctgacg taatcctgtt gcgcttctgc tgtggcgatg gcggcaagca gggcttcgtg
     9061 tttttcctgt tctttggtca aatcgcaggc gatgatttct tcggtggact cgccaatcag
     9121 aagcttgccc agttcttgca ggttcggcag accttcgagg aacagaatgc gctcgatcaa
     9181 atcgtcggca gctttcattt caacgatgga ctgtttgaag aaatgttcgc ccagttcttc
     9241 aaagccccag tttttcaaaa tacgggcgtg aaggaaatat tggttgatgg ttaccagcag
     9301 caagcctaag tttttgttta gctcgcggat aaccaaacgg tcgcctttca tacggactcc
     9361 ttttattccg aatacggatt acagttggct ttggtagtag ttgccttcgc caatcagctc
     9421 gatcaggcgc agctgctgtt ccagccagtg ggcgtggtct tcttcggtat ctttcagttg
     9481 ggcaaccatc aggtcgcgcg taacatagtc ttgagcctct tcgcacagtt tgatgccttt
     9541 tttcaacgca tcacgtactt catattcggt ttgcaggtcg gctttgaggc aggaaaccac
     9601 gtccgtgccg atattcagtt cggcgcgtgc cattttcggc gtaccgccca gcatcaggat
     9661 gcggcggatg aagtcttcgg cgtgtgtggt ttcttcttcc atctcgtggt tgagacgttc
     9721 aaaaagtttg gtgtagcccc attcggagta gaggcgggag tggataaagt attggtcgcg
     9781 tgccgccagc tcgccagaca gcaattcgtt catataatca acaacagctt gattgccttg
     9841 cataatatct ctctttctgt aacttggttt tcggtatgtc tgaacagcat ctgtgtcgct
     9901 attcgggatg cgtgcattgt atgcaaaagc tgtcggcatg acaaattttc tatttaaaat
     9961 acaaacaatt atcaaaagaa atagggcgta ttccccgatg ccttccaaat cagtatgctg
    10021 tttcttatcg gtttttttgc tgctaaaact aagaatccat ctcatccata aagaacattt
    10081 acacttgtta accatagcta aaagcaaata ctgtacggtt ttttatcaaa atttataatg
    10141 aatcgttctc attaaccgac agattctttt gagattatcg gattttggga ataaattatg
    10201 ccgtctgagg ggctttcaga cggcatagac aattaaagct gattaaacaa cgcggcagac
    10261 agcagcgtag gaaattattt tttacttcgg aacggtattt tgggtttaat gatgcccgca
    10321 accgccgtgg ctttcgcgta aggcttcggc agcctgttcg tcggcgtggt agctggaacg
    10381 taccatcgcg ccgatggcgg cattgctgaa gcccagttcg tatgcttctt tttcaaagat
    10441 tttgaactgc tcgggcgtaa cgtagcgcag gacgggcagg tgtccgtctg aaggctggag
    10501 gtactgtccg atggtaatca tttcgatatt gtgcgcccgc atatcgcgca taatttcacg
    10561 cacgtcttcg tctgtttcgc ccaagccgac catgatgccg gatttggtcg ggatgtgcgg
    10621 catcatttct ttataacgtt ttaataagtc taaagaatgt tgataattgg caccgggacg
    10681 ggcttttctg tacaggctcg gatgggtttc taggttgtgg ttcatcacgt cgggcggggt
    10741 ttcggcaagg attttgagtg cgatgtccaa gcgtcctcgg aagtcgggga cgaggatttc
    10801 gattttggtg ttcgggctgg tttcgcggat ggctttgatg cagtcggcga aatgctgtgc
    10861 gccgccgtcg cgcaggtcgt cgcggtcgac ggaggtgatg acgacgtaac gcaggttcat
    10921 ggctttgacg gattcggcga ggtttttcgg ttcgtcgggg tcgagcatat tgggccgacc
    10981 gtgtcccacg tcgcagaacg ggcagcggcg ggtgcagata tcgcccataa tcatgaaggt
    11041 cgccgtgcct ttgctgaagc attcgccgat gttggggcag gaggcttcct cgcaaacggt
    11101 gtgcatcttt tgttcgcgca aaatgtcttt gatttcaaag aatttgcgcg atgggagttt
    11161 ggcgcgtatc cattcgggct ttttcagttt ttcctgaagg gggacgactt tgatggggat
    11221 gcgcgcggtt ttgtccgcgc ctctgagttt gatgccgcgt ttggggtcgt cggttttgat
    11281 ttcactcatt gttatctgct ttcggtgtga attgtgtttc aaggtgtgcg gtaagtttgg
    11341 cggcgacttc gtccggcgtg gggcagggtt ggacaaaatc cgcgatttgc gtcatttcca
    11401 taccggcgta gccgcagggg ttgatgtggg taaacgggct taaatccata ttgacgttga
    11461 gcgcaaggcc gtgatagacg gaacctcctt tgatacgcag ccccagtgag gcgattttgc
    11521 gttcgccgac gtaaacgccg gggcgtttgg gatctgccgc cgcttcgatg ccgtattccg
    11581 ccaatgtggc gatgatgctg ttttcaagcg cggaaacaat gttgcggacg ctggttttgc
    11641 gccgtttgaa atcaatcatc gtataaacga ccaattgccc gggtccgtga taggtaatct
    11701 gcccgccccg gtcgatttgg acgatgggaa tgtcgtcgcg gatcagcagg tgttcgggtt
    11761 tgccggccag cccctgtgtg aacacggggg gatgttctac cacccacaat tcatcttcgg
    11821 tagaggcggt acggttggcg ttgaaagttt tcattgcctc aaaagtcggc agatattcaa
    11881 ccaaaccttt atgtatgatt ttcatctcaa agtaccactt tgaccaattt gtgcgaagtc
    11941 agcgcgcggt agatattgtc aagttgttct tggttttcaa cctttacttg tacggtggcg
    12001 ccggtatagt tgcctttgct gctcggacgc gtggtgatgt ggtgcgcctg cgtgtcgggg
    12061 gcgtggaggc ggacggtgtc caataccgcc tgctcgaact cgggatgcac cgcgcccatt
    12121 actttcaatg ggaaggtgca gggaaattcg atgagggatg ttttgttttt ttgttcggtc
    12181 atgatgtgct gccttgtcgt gtacggtatg ccgtctgaag gcgggtttgc ctttcagacg
    12241 gcatcggatg tgcgttattt tagcctaaac cgcgataaca ggctatcggg aaggcggagg
    12301 cttttttgac ggcgcggcgg ttctgctata ctggcgcaca atattatttt tagaagggtg
    12361 gtttttatgt atcggaggaa agggcggggc atcaagccgt ggatgggtgc cggtgcggcg
    12421 tttgccgcct tggtctggct ggttttcgcg ctcggcgata ctttgactcc gtttgcggtt
    12481 gcggcggtgc tggcgtatgt attggaccct ttggtcgaat ggttgcagaa aaagggtttg
    12541 aaccgtgcat ccgcttcgat gtctgtgatg gtgttttcct tgattttgtt gttggcatta
    12601 ttgttgatta ttgtccctat gctggtcggg cagttcaaca atttggcatc gcgcctgccc
    12661 caattaatcg gttttatgca gaacacgctg ctgccgtggt tgaaaaatac aatcggcgga
    12721 tatgtggaaa tcgatcaggc atctattatt gcgtggcttc aggcgcatac gggcgagttg
    12781 agcaacgcgc ttaaggcgtg gtttcccgtt ttgatgaggc agggcggcaa tattgtcagc
    12841 agtatcggca acctgctgct gcttcccttg ctgctttact atttcctgct ggattggcag
    12901 cggtggtcgt gcggcattgc caaactggtt ccgaggcgtt ttgccggtgc ttatacgcgc
    12961 attacaggca atttgaacga ggtattgggc gaatttttgc gcgggcagct tctggtgatg
    13021 ctgattatgg gtttggttta cggcttgggg ttggtgctgg tcgggctgga ttcggggttt
    13081 gcaatcggta tggttgccgg tattttggtt tttgttccct atttgggcgc gtttacagga
    13141 ctgctgctgg caaccgtcgc cgccttgctc cagttcggtt cgtggaacgg catcttggct
    13201 gtttgggcgg tttttgccgt aggacagttt ctcgaaagtt ttttcattac gccgaaaatc
    13261 gtgggagacc gtatcgggct gtcgccgttt tgggttatct tttcgctgat ggcgttcggg
    13321 cagctgatgg gctttgtcgg aatgttggca ggattgcctt tggccgccgt aaccttggtc
    13381 ttgcttcgcg agggcgtgca gaaatatttt gccggcagtt tttaccgggg caggtaggcg
    13441 gttccgaaac atatttgaag cggaatacaa ccctgcccga gt
//
LOCUS       DAWKST010000046        13390 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.046, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000046 DAWKST010000000
VERSION     DAWKST010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13390
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.046"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(110..538)
                     /locus_tag="WJL87_000912"
     CDS             complement(110..538)
                     /locus_tag="WJL87_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ7799354.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(603..2351)
                     /locus_tag="WJL87_000913"
     CDS             complement(603..2351)
                     /locus_tag="WJL87_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ7799355.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTLMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPPAGDFGARFKQNTDHIVLTHVFNSGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2678..4111
                     /gene="ccoN"
                     /locus_tag="WJL87_000914"
     CDS             2678..4111
                     /gene="ccoN"
                     /locus_tag="WJL87_000914"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ7799356.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4138..4749
                     /gene="ccoO"
                     /locus_tag="WJL87_000915"
     CDS             4138..4749
                     /gene="ccoO"
                     /locus_tag="WJL87_000915"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ7799357.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4754..4924
                     /locus_tag="WJL87_000916"
     CDS             4754..4924
                     /locus_tag="WJL87_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ7799358.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4948..6045
                     /gene="ccoP"
                     /locus_tag="WJL87_000917"
     CDS             4948..6045
                     /gene="ccoP"
                     /locus_tag="WJL87_000917"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ7799359.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKAPVKKAEPAPTPAPAAEPAASAPA
                     EAAQAASEAKPAAAEPKAEEKAAPAAKADGK"
     gene            6085..6279
                     /locus_tag="WJL87_000918"
     CDS             6085..6279
                     /locus_tag="WJL87_000918"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ7799360.1"
                     /translation="MPGIPHVGIKADWADRIKKGKDTLHKHAIEGFNTMPAKGGRGDL
                     SDDEVKAAVDYMVNQSGGKF"
     gene            6481..7890
                     /locus_tag="WJL87_000919"
     CDS             6481..7890
                     /locus_tag="WJL87_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ7799361.1"
                     /translation="MDTQIKTEADNQSNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYAMDEIHSDMMLGSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPPKGRARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7992..11201
                     /gene="recC"
                     /locus_tag="WJL87_000920"
     CDS             7992..11201
                     /gene="recC"
                     /locus_tag="WJL87_000920"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ7799362.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSVLQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILS
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVGTDDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVRWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTAMFGRFAAFLETLSDIVRIWRQPATVDEWVGRCRGLLETLFQAEADDQKAVQNLE
                     NEWVKWQAETELAQFSGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMTGTNSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYATALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAETYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQNFQTAAKQIDTAVLNSPKLPPLSYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLARLLADTLAAAEKREGAGAA"
     gene            complement(11271..12839)
                     /gene="mtrF"
                     /locus_tag="WJL87_000921"
     CDS             complement(11271..12839)
                     /gene="mtrF"
                     /locus_tag="WJL87_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ7799363.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTLYPAP"
     gene            13173..13385
                     /locus_tag="WJL87_000922"
     CDS             13173..13385
                     /locus_tag="WJL87_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799364.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
BASE COUNT         3358 a         3194 c         3555 g         3283 t
ORIGIN      
        1 actgtaaaaa cggagaagga cagaggacgg cagtaaggtt tgccgtcctc tttttcaagt
       61 tgcggtggaa tgccgcttga aggtgtggtt taaacgtttc ggcttgtaat cagacgcggg
      121 cgttgtccag ccgctctctg aagttttcca aatttacgcc tgccgcttgg gcgcggcgga
      181 tgatttcgtc aaccggcata ccttctgccg cccggcggaa gccccacagg agggagcagc
      241 gcgtaccggt ccggcaatag gcaaggacgg gatattcggc ttgtccgatg agttggcgga
      301 aggtttcgac atcgtgtttt tggatgtcgc gtgcggtaac gggttggtga tggaatccag
      361 taacgcctgc ttgttccagc cactgtttga tttgggcgaa gtcgggttgc gattcttctt
      421 cgcggtcggg gcggttgcag atgacggttt tgatgcccag ttgcgcgatt tgttccgcat
      481 cggctttggt cagttgcgga gaaatataga ggtgttcgtc aagtttcaga atggccattt
      541 tgtttccttt actttgcggt tgtttttcag acggcatttt gtgtgcaatg ccgtctgaaa
      601 gtttaaccga acaaccagtt gtccaacagg ttccggtctg tgatatgtag gatggcagta
      661 tccgctgccg ctgcctgaac cgtcaagacg gtttgacgca atatgccggc acggaagaag
      721 tggatattga tttttgcatt gacgtggtat cgggcccatt gtgcggtaaa gtcggtgcag
      781 gcataaccgt ctaaagcaat gattttgtct tgcgggcaca gtgccgcaga ttccgcgctg
      841 ccgctgttga agacatgggt caggacgatg tggtcggtgt tttgtttgaa acgtgcgcca
      901 aaatcgcctg ccggcgggac ggggcagatg tgttctgcgt atccgccgcc gtgttgtcgg
      961 ggaagtggca ggaaggtcag tcccacgcct gcggttgcca ggcattcggc aagcggcaaa
     1021 tcttcggtac tgtataacgc tttttggaaa aaatcttcca aatccaagcc ggtaatttcc
     1081 tgacagcgga tttgccagtg tttttccgga atacccgagt gtgtgtccct ccactcccga
     1141 tagagtttgt ccattaacgt atcgagagaa tgtctgccgt tgcttcggtt gcgtattatc
     1201 agatcaaggc acaatgcggc aagcgcgcct ttctggtagt agctgacgat ggcgttgggg
     1261 ctgttttcat ccggtttgta aaatttgttc cacgcggtaa aactcgattc cgccaaggtc
     1321 tgcctcaaac ggccgcgggt ttgttgtacg cgcgtaatgc cttgtgccag caggtttaaa
     1381 taagattcgg gcgagatggt gcggctgcgt gccaaaaaca aatcgtcgta ataggatgta
     1441 ataccttcga atgcccatag ttgttcggta tagttttctt tgtcgaggtc ataagggacg
     1501 aacgcggcag gtttgatgga tttgacgttc cacgcgtgaa aatattcgtg ggagaaaagt
     1561 ccgagcaatg tggtgtaggt atcgtcggca tcggtcatac cgtacggcgg aaggctgtgg
     1621 cggtcggcga gcagggcggt gctgtcggtg tgttccaaac cgccgtaaat atggtcgccg
     1681 acgtggagca ggaacaaata tttttgaaac ggggcagggg aggaaaacac cgccagttct
     1741 gtttcgcaga tttttttgat atccgaaacc agcctgttgc ggtcgaaatc gggatatatg
     1801 ccgcttaagg caattgtgtg cggaatgcct gccgcctcaa aatctaaaaa ttcaatcaag
     1861 cccatctcga caggtcggtc aatcaattcg gcataagatg ccgcctgaaa gacaaacctc
     1921 cctgtttccg gcagcgttgt ggcaatacgc cattcggacg gaatacccgt caattccaag
     1981 cggtgcggca gcgtttccgt tccttcgact ttcaaaaaca ggcacgatcc gtcaaaaaaa
     2041 ccgcgttctg tcgtcaggaa agaacctcga accgacaaat cgaatgcata tacggtgtag
     2101 cggatttgcc actcgccgcg tacggcggca gcgtgccagc ggttttttgc aatttgttcg
     2161 agcggcatgg acgtgccgtt acaggatgca tggatagaag tgatgtggcg ggaaaaatcc
     2221 cgaatcagat agctgcccgg tacccaattc ggcaggctta tttctatagg aagatcatca
     2281 tcttgtgtga atgtcagcag gatgtgccac tcgtggtcga gaggggacgg ggttatttta
     2341 taatgaatca taactatcaa atatatcgtt aaacgggatg gattaaatac ctttaaaatg
     2401 cagcttaacc tacatcaata aagtgccaca aaaagggaaa attggaaaca aaagcaatgg
     2461 cggtcgggtt gtaatttatt ataacaatat gatttatatg taataaaaaa cagattaagc
     2521 aaagggctcg tcggcggcgc gaatgtaatt ccgcttggcg taataaggca gatatgttaa
     2581 atttagaaaa aattacgctt gcccggattg caccttaaac aggctgcgga agcggcagga
     2641 taaaaatctt tattcccata aattcatgga gactctcatg gacacacaaa cttacaacta
     2701 caaagtggtg cgccagttcg ccatcatgac tgtagtttgg gggattgtgg gtatgttggt
     2761 cggcgttatc gtcgccgccc agctttttgc tcctgccctc gacttgtcta atatcggacc
     2821 ttggttccac ttcggccgcc tgcgtccgct gcacaccaat gcggttattt tcgcatttgg
     2881 cggttgtggc ctgatcggca catcatacta cgttgtccaa cgtacttgta atacccgtct
     2941 tttcggcggt tggctgccgg catttacctt ttggggctgg caggcggtaa tcgttgccgc
     3001 cgtcgtcagc ttccctatgg gttggaccca aggtaaggaa tatgccgaac tggaatggcc
     3061 gatcgatatt ctgattactt tggtttgggt ggcttacgcc atcgtattct tcggtacgat
     3121 tgccaaacgt aagattaaac atatttacgt tgccaactgg ttctacggcg gctttatttt
     3181 ggcggtcgca cttttacaca tcgtcaacaa tatcagcatc ccggccggtt tgatgaagtc
     3241 ataccccgtc tattccggtg cgattgatgc tatggttcaa tggtggtacg ggcataacgc
     3301 cgtgggcttc ttcctgactg ccggcttctt gggtatgatg tactatttcg tacccaaaca
     3361 agcagcccgc cccgtttact cctaccgcct gtccgtcgtt cacttctggg cgttgatttt
     3421 cacctatatg tgggcgggtc cgcaccatct tcactacact gcgctgcctg actggacgca
     3481 atctttgggt atggttctgt ctttgattct gttcgcaccc tcttggggcg gtatgattaa
     3541 cggcatcatg accttgtccg gcgcgtggga caaactgcgt acagacccga ttcttaaatt
     3601 cctgattgta tccttgtcct tctacggtat gtctaccttt gaaggcccga tgatgtcgat
     3661 taaaacggtc aatgcattga gccactatac ggactggacc gtcgcgcacg ttcatgcggg
     3721 tgcgttgggc tgggtaggct ttgtaaccat cggttccgtc tattacatga ttccccgtct
     3781 gttcggcaaa gaacagatgc acagcaccaa gctggtagaa gcacattttt ggattgcgac
     3841 catcggcgtg gttctgtata tcgctgccat gtggattgcc ggtgtgatgc agggtttgat
     3901 gtggagttct ttgaacgatg acggtacgct gacctactcg tttgtcgaat ccgtaaaacg
     3961 caccatgcct tactacgtga tccgttttgc aggtggttta ttgtatctga gtggtatgtg
     4021 cattatggcg tacaacgtgt accgcacagc catcggtggt aaagcagtcg atgccgaaat
     4081 ccctgcggtt tcccaaacac agcaccacta aaatactaag aaagataggc taccaaaatg
     4141 aaattacaac aattggctga agaaaaaatc ggcgttctga ttgtgttcac gctgcttgta
     4201 gtcagtgtcg gtctgttgat tgaagttgtg cccttggcct ttaccaaggc ggcaacacag
     4261 ccggcaccgg gcgtgaagcc ttacaatgcc ttgcaggttg ccggacgcga tatttacatc
     4321 cgtgagggct gttacaactg ccactctcaa atgattcgtc cgttccgtgc ggaaaccgag
     4381 cgttacggtc attactctgt tgccggagag tcggtttacg accatccttt ccaatggggt
     4441 tccaaacgta ccggtcctga tttggcacgt gtgggcggcc gctattccga cgaatggcac
     4501 cgtatccacc tgctgaatcc ccgtgatgtc gtgcctgagt ccaatatgcc ggcattccca
     4561 tggcttgcac gcaataaagt cgatgtcgat gcaaccgttg ccaacatgaa ggctttgcgt
     4621 aaagtaggta ctccttacag tgatgaggaa attgcgaaag cgcctgaggc tttggcaaac
     4681 aaatccgagc tggatgctgt agtcgcctat ctgcaaggat tgggtctggc tttgaaaaac
     4741 gtaaggtaac atcatggata ttaacggtat tcgcgctctt ttcacggtat ggatctttat
     4801 ctgtttcctg ttggtactct atatcgtctt caacaggcgg aataagaaaa actacgataa
     4861 tgccgcaaac agcatttttg atgaaaacca agatgcgcaa gataagaaaa gcgaaaaccg
     4921 ttaatattgt gataacggag caaaacaatg aacacaacat cccaatttac cagtaatttc
     4981 tggaatatat atattgcagt tattgtctta ctgagcttta tcgctttggc ttggctgctg
     5041 ctgtctcaaa atgttgtcaa acgtccgaag aagggcgaag aagtacaaac tacgggtcat
     5101 gagtgggacg gcattgccga atacgacaat ccgctgcccc gctggtggtt ttggctgtgt
     5161 gttttgacgt ggctgttcgg tatcggttat ctggttatgt atccgggtgt cggcgactac
     5221 aaaggtctgc tgaaatggac cagccataac caatatgaaa aagaggtcaa aaaagccgat
     5281 gagcaatacg gcaaactgta tgccaagttt gcggatatgc cgattgaaaa agtggcaaaa
     5341 gaccctcaag ccaagcaaat cgcccaaaac ctgtttaaca cttattgtat ccagtgccac
     5401 ggctctgatg ctaaaggctc taaaggtttt ccgaatctga ccgatagcga ttggttgtgg
     5461 ggcggtgatc ccgataaaat ccacgagacc atcgaaaaag gccgtgttgc gactatgcct
     5521 gcctggggcc ctgctttggg cgaagaaggc gtgaaagacg ttgcccatta tgtgatgtcc
     5581 ctttcaaaac ccgaaggtca gtatgatgag gaacgtgccg cgcgcggaca agccttgttc
     5641 agcggtccgc ctgccaactg tttcacttgc cacggcgata agggacaagg tatccaaggt
     5701 ttgggtccga acctgactga tgacgtgtgg ttgtggggcg gtacgcaaaa atccattatc
     5761 gaaaccatta ccaacggtcg cagcagccaa atgcccgctt ggggacattt cttggataaa
     5821 gacaaactgc atattatgac tgcttatgta tggggtcttt ccgataaaga cggtaaagct
     5881 ccggtgaaaa aagccgagcc tgcaccgact cccgcaccgg cggcagaacc cgcagcctct
     5941 gctcctgcag aagcagcaca agccgcgtcc gaagccaaac ctgccgcagc agaacctaaa
     6001 gccgaggaaa aagctgcacc tgctgccaaa gcggacggca aataggttta tgaaaccgtt
     6061 tgtgccgcct gccatggcaa tgcgattccg ggtattcccc atgtaggcat caaagccgat
     6121 tgggccgacc gcatcaaaaa aggcaaggac acgttgcaca aacacgcgat cgaaggtttc
     6181 aatacgatgc ccgcaaaagg cggtcgcggc gatttgagcg atgatgaggt taaagctgcg
     6241 gttgactata tggtcaacca gtcaggcggc aaattctaac ttgactgagt ttccgatatt
     6301 atcggttatg aaaatccaga ttcagtattg aatctggatt ttttgtttta acgggcatcc
     6361 ggttttaaac gcagactgcc tatttgttgt aatgaatttt gactttagtc ataaagaatt
     6421 agtctttatt gtttataatc gcgcatcatc acgatagccc tattttcaaa aggacgatta
     6481 atggatacac aaatcaaaac tgaagccgac aatcagagca accggcgtta tctgaccgtt
     6541 tggcggtggc atttttatgc cggtctgctg gttatgcctt ttcttaccct gcttgccgtt
     6601 acgggtttgg gtatgctgct gtttgccaat attaccggta aggagggcga gcggattcat
     6661 gttgtgccgc aggcaacggt acaacctctg tctgttcagg cggaagcggc acgcagtgcc
     6721 gttaatccgg aaacttcgtc cgtcgttcag tatattgcgc cgcgtgccga tgatatggtt
     6781 gccgtgttcc gtgtcaacaa tgagggcaag gcaacgatgg tcgcggtcga tccttatacg
     6841 gcaaaagtgg tcagtaccat gccgcgcaat cagggttggt attacgcgat ggatgaaatc
     6901 cacagcgata tgatgctcgg ttcgacaggt gattatcttt tggaaacggc tgcatcgctg
     6961 acgattatca tgataatcag cggtttgtac ctttggtggg tgaaacggcg cggcatcaag
     7021 gcgatgctgc tgccgccaaa aggcagggcg cgttcttggt ggcggaatct gcacggcgcg
     7081 tttggaactt gggtgtcgtt gattttactg ttgttctgcc tgtcgggtat tgcttgggca
     7141 ggtatttggg gcggcaagtt cgtgcaggct tggagtcagt tcccggcagg caaatggggt
     7201 gtcgaaccga accctgtttc agtcgtgccg acccacggcg aggtattgaa tgacggcaag
     7261 gttaaggaag tgccgtgggt tttggagctt acgcctatgc ctgtttcagg gacgactgtg
     7321 ggcaaagacg gtattaaccc tgacgagccg atgacattgg aaaccgtcga ccgttttgcg
     7381 cgggaaatcg gtttcaaagg gcgttatcag ttgaatttgc ccaaaggcga ggacggagtg
     7441 tggactttgt cccaagactc tatgagttac gatatgatta gtccgtttgc cgaccgcacg
     7501 gtgcatatcg accagtacag cggcaaaatc cttgccgaca tccgctttga cgattacaac
     7561 ccgttcggca aatttatggc ggcaagcatt gcgctgcata tgggtacttt gggctggtgg
     7621 agcgtgttgg cgaacgtttt gttctgcctt gccgtcattt ttatcggtat cagcggctgc
     7681 gtgatgtggt ggaaacgccg tccgaccgga gcggtgggca tcgttccgcc ggcgcagaaa
     7741 gtcaagctgc cggtttggtg gatgatggca ttgccgctat tggcaatcgc actgctcttc
     7801 ccgaccgcac tgcttgccat tgccgtgatt tggctgttgg ataccttgct gctgtcgcgg
     7861 attcctgttt tgaggagatg gtttaaatga cccgatgccg tctgaagcgt tcagacggca
     7921 ttttgtttga aggcgggcgg gggaaaggct atataatccc gaatacttga ccacagcttc
     7981 tgtttgaaat catgttttat ctgtatcaat ccaaccgtct tgaaacgctg gcggcactgt
     8041 ttgcccgcat tcaaaaagtc aaaccgctga aatcggtttt acagcccgaa cagattattg
     8101 tgcagagtca ggggatgcgc cgctacctca atacctgcct cgcccgcgat ttgggcgtgg
     8161 cggcgaattt gtcgttcagc ctgcccgccg gtctgacgtg gaagctgatg aaaaaactga
     8221 ttcccggtat tccggaactc agcccgtttg cgcctgaagt gatgcgctgg cggctgttgg
     8281 atttgttccg cagcgaggca tttcggaata cggcagaatt tgaagatgtg aggaatgtgc
     8341 tgcaagacta tctaggcagc ggcgaatcgg cagattatca gcttgcggga cagcttgcgg
     8401 acatattcga ccaatacctt gtctatcgtc cgcagtggat agacgcgtgg cagcagggca
     8461 ggatactcag tttgggcgac gatgaaatct ggcagtccaa actgtggcgt tatcttgatg
     8521 acggcaggca gagcgcgccg caccgtgttg cgttgtggga aaagctgttg gaatctttga
     8581 gcagtgataa gctgcccgag cgttatttcg tgttcggcat ttccacgatg gcgccgatgt
     8641 atttgcagct tttgcacaag ctgtccgaac attgcgatgt gttcgtgttt gcactcaatc
     8701 cgagtgggat gtattggggc aacgtcatcg aagcggcgca aatcctcaaa ggcggcggcg
     8761 atcccgattt aactcaggca gggcatccgc tgctcgcctc attgggcaag caggggcgcg
     8821 actttttcga ctttttgaac gaaatggaaa tagaaggaga aacgccggta tttgaagaag
     8881 gcgggcgcga tacgcttttg cacgccctgc aaaccgatat tcaaaacctg aaaatgccgt
     8941 ctgaaaatgt gggaagcgtc ggtacggatg acggttcgat acgtatcgtc tccgcgcaca
     9001 gccctttgcg cgaattgcag atactcaaag acaagctgtt gaaaattctg catgaacatc
     9061 ccgattggca gccgcatgat atcgccgtat taaccccgaa tatcgaatcc tatacgcctt
     9121 ttatcgaggc cgtgttcgga caggcgcagc ccggtgcgca ggcattgccg tattccgttt
     9181 cggacgtgaa aataagccgc cgacaaccgc tgtttcatgc tttgtcatgc ctgttcgact
     9241 tgttggaaag ccgatttgaa gtcgataaag tgcttgtgct tttagaaacc gcacccgtgt
     9301 tgcgccgttt cggactgact gaggacgatt tgccgctttt gcacgacatg gttgccgatt
     9361 tgaacgtccg ctggggtttg gacggagaaa tgcgcggcgg cacggatcag ctgtttacct
     9421 ggaagcaggc ggtagaacgc atgatattgg gctggatgct gcccgaaggc ggcaatccga
     9481 tgtggcagga tgtcagcgcg tggtatgccg acgtgaatca aaccgccatg ttcggacgtt
     9541 ttgccgcctt cctcgaaacc ctgtcggata ttgtacggat atggcggcaa cctgcgacgg
     9601 ttgatgaatg ggtaggacgt tgccgaggtt tgcttgaaac attgttccaa gccgaagccg
     9661 atgaccaaaa ggcagtccaa aacctcgaaa acgaatgggt caaatggcag gcggaaacag
     9721 aattggcgca attttccgga cagttgccgc aacaaaccgt catccgccat atccgacgtt
     9781 tcctcgacag cgaaagcgag gcaggctttt tacgcggcgg catcaccttt tgcagtatgg
     9841 tgccgatgcg gagcctgccg ttcaaagtca tctgcctgtt gggtttgaac gacggagatt
     9901 ttccccgcaa taccaaagcc gccgtattcg acctgattgc caaacatccc gccaaaggcg
     9961 accgagcccg tcgcgatgac gaccgctacc tgttccttga agctctcatc agcgcgcgtg
    10021 aaatcctgta tttgtcctac atcgggcgcg atatccgcaa agacgaagag cttgccccgt
    10081 cttcgctgtt gggcgaactc atcgacacag ttgccgccat gaccggcaca aacagccgcc
    10141 aacttgcaca aaattggata gaacagcatc cgctgcaagc cttctcgcgc cgatatttcc
    10201 aagaaggcgg acgttcagac ggcatattcg gcacgcgtac cgactacgcc actgcgctcg
    10261 gacaaacgcc cgaaccgccg caaccctttt tcgaccaacc cgtagaaaac gccgaacctg
    10321 ttgccgaaat cggacaggac gaatttatcc gtttctggcg caaccccgtc aaagtatggc
    10381 ttcagcagca gcttgcgtgg agcgaacccc atatcggcga agcctgggag cctgccgaac
    10441 ccttcgagcc gcaacacgcc gatcaaatcg ccgaaaccta catcgaagca cggcgcgaag
    10501 gacgggattt tgcccaaacc gccgcccgca tcggggcgga aagcctcctg ccgtcgggag
    10561 agttgggcag actttggcag cagaacttcc aaactgctgc caaacaaatc gacacggcgg
    10621 ttttaaacag ccccaaactg ccaccgcttt catatgccat accgtcggac gggcaaatcc
    10681 tgaaaggcag cttgggcaat ctgtaccgct gcggacaagt gttttacgcc tacggcaaac
    10741 ccaacgcgcc gcaacgtatc gcttttctgc tggaacacct gatattttgc gccgttatgc
    10801 cgtctgaagc cgaaacgcgg caaaccttta tcgtccaatc cggagaaacc gaaatattgg
    10861 cggaaatcgc gcaagacagg gcattgcagc tattgtcgga atggatggcg tttttcaata
    10921 tcgggcaaaa ccgcccgctg ccgttttttg ccaagacctc gcttgccgct gccgaagcgt
    10981 ttgcccaaaa acaagattgg gaagccgccc tgaaaaaagc ccaaaccgcc tattacggca
    11041 acaaagtcag caaagggcag aaagactata ccgaagtcgc cctcgtgttc ggcaacgcaa
    11101 gccaaaaccc actcgaacaa cccctgtttg aaaacctcgc ccgcctgctt gccgacacgc
    11161 ttgccgcagc ggaaaaaagg gaaggggccg gagcagcctg acgggatgga aaggcaaatg
    11221 ccgtctgaaa acagcaaaaa ggttttcaga cggcattttg tttatcgtgt ttaaggtgcg
    11281 ggatacaaag tgggtgcgcc gggaccgacg ggcaggccca aaacaaatac ccaaatgcag
    11341 aataaggcaa tccatgcaat taagaagaaa gcggaatacg gcaacatcat agaaatcagc
    11401 gtacccacgc ccgcatcttt tttgtatttg atcactgtcg ccataatcag cccgaaataa
    11461 ctcatcatcg gcgtaataat attggtaacg gaatcaccga tgcggtaagc ggcttgaatg
    11521 acttcgggcg cgtagccggc caacatcagc atagggacga aaatcggcgc agttaccgcc
    11581 cattgcgcgg aggcggagcc tatcatcaga ttgataaaag cacaaattaa aataaaaccg
    11641 ataaacaaca cgctgccgcc caagccgact tcttttaaga acgtcgcccc tttaacggca
    11701 atatattgcc caatattcgt ccaattaaaa aatgcgacaa attgtgcggc aaaaaagatg
    11761 atgaccaaat aaagccccag agtactcatc gattcggcca tcgcattaac gacttcctgt
    11821 tcgccgcgca aacttcgggt tacccggcca taaacaatgc ccggcagcgc aaacaacaag
    11881 aaaataaaaa caacaatcga ttttaaaaac ggcgaaccgg aaaccaatcc tgtttcagga
    11941 tgacgcaaaa taccgtcggc agggacgatg ctccaagcca atagggcgga taaggcaaca
    12001 aacaccacgc cagcccaaat taatccttta tattccaaag gcgtgatttc attggaatgc
    12061 cgaatgtctt tttcttcttg tgacaaatct gattgataag ggcccaattg cggttcgacg
    12121 attttttcag taacaaaata accaatcaaa gcaatcacaa acgtactggc taccataaaa
    12181 aaccagttgg cttcagggcc tacgacgtag tcgggatgga tgatttgcgc cgcctgttgg
    12241 gtgatgcctg ccaagagcgg atcgattgtg cctaagaaca gattggccga ataaccgccc
    12301 gaaacgccgg cgaaagccgc agccagaccg gcaagcggat ggcggccgag ggaatgaaag
    12361 atgatggcgg acaaagggat taggacgaca tagcccaatt cagaagcggt attagataaa
    12421 atccctgtaa aaacaaccat aaaagtagtg agtttgcgtg gagattttgt gagcaataag
    12481 cgcattaatg cggaaatcaa gcccgatttt tccgcaatcc ccacgcccaa taaagaaacc
    12541 aacaccgttc ccaacggcgc gaaaccggtg aaatttttaa cggtatgcgt caggattttg
    12601 atcaaaccgt cagcatcgag caggctgaca acgtgaatca aaccgtcatc ggcacgtcct
    12661 ttcgcaccaa cagggcgcgg atcggggacg gatagtccga aatacgcacc ggcggcagag
    12721 gcaatcagca ataacacaat gaaaataata aaaagcgtaa ccgggtgcgg caacatattg
    12781 cccagccatt cgactgtgcg taaaaatcgt ccgtcccgtt gcgtatcggt ttgactcatc
    12841 tgcttctcct taaaaaaaat atattgagtg cattaaattt ttgaatgtaa catgaagtgt
    12901 tttgtaagca aactaaaata atcgtatatg tatataaccg taatagatta aaacagaaaa
    12961 tgcccacatc gttattctca taaaggcggt aatccgaaaa cttgacgtta aagccttatc
    13021 aggaatgact ggaactcaac aaagcggttt cccgctttcg cggaaatggc gggattttcg
    13081 gtttttgttt gcaaaaatgc cggagaaaaa tgcagtttac attttgaatg gtattgttat
    13141 taattgtaaa taaatattgg aaggtaaatg atatgaaaaa tgaaattcaa aaaattatgg
    13201 acaaatatga tccatggcat gaagatgatt ttgagtcgta tgaagacatt gccaaagatg
    13261 tatcgttgat gacagataaa acgttcattg aacattattt gttagaagtt tattcagaag
    13321 aaaacggaca ttttgaccaa gagaatgtcc atgcaatgat agaagaaatt aaaaatgcaa
    13381 tttagtgaat
//
LOCUS       DAWKST010000047        13346 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.047, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000047 DAWKST010000000
VERSION     DAWKST010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13346
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.047"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(24..359)
                     /locus_tag="WJL87_000923"
     CDS             complement(24..359)
                     /locus_tag="WJL87_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ7799365.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQGL
                     PPGVGDFFR"
     gene            complement(439..2568)
                     /gene="dnaX"
                     /locus_tag="WJL87_000924"
     CDS             complement(439..2568)
                     /gene="dnaX"
                     /locus_tag="WJL87_000924"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ7799366.1"
                     /translation="MAYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCESCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLHNMTSQQVADHLAHVLDSEKIAYEPPALQLLGRAAAGSMRDAL
                     SLLDQAIAMGSGSVAEQDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAQEMAACAV
                     GFDNALGELAILLQHLALIQAVPSALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLSLAPDEYAGFMMTLLRMLAFAPLAAASCDTNAVIENTELKSPSAQTAEKETAVK
                     KPQPRPEAETAQTPVQTASAAAMPSEGKTAGPVSNQENNDVPPWEDAPDEAQAAAGTA
                     QTSAKSIQTASEAETPPENQVSKNKAADNVTDAPLSEVPSENPIQATPNDEAVETETF
                     AHEAPAEPFYGYGFPDNDCPVEDGAEIPPPDWEHAAPADTAGGGADEEAEAGGIGGNN
                     TPSAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARAT
                     ADKKRLDKIRDTLAQAYGLQLTLQTQDWRDEAGRETPAMQDKRVQAEDRQKAQALLEA
                     DPAAQKILQAFGAQWQPESLELAANRP"
     misc_feature    complement(1340..1379)
                     /gene="dnaX"
                     /locus_tag="WJL87_000924"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            complement(2663..4639)
                     /gene="mutL"
                     /locus_tag="WJL87_000925"
     CDS             complement(2663..4639)
                     /gene="mutL"
                     /locus_tag="WJL87_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ7799367.1"
                     /translation="MSRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELAGGGIRLIRVSDNGGGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSEATEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSDNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSQQVHQLVFHTLNKALADTRADLTE
                     SVSNAGEVLHDITGVTPAPMPSENNSENLFDRASNYPTGNKSDTHNAFGSSGKTAPMP
                     YQSAYAPQQRSLSLRESRAAMNTYAELYKKTDDIDLELSRFEQARFGNMPSETPAPQT
                     DTPLSDGIPSPSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGNLQSQRLLIPVTFAASHEECAALADHAEALAGFGLELSDMGGNTLAVRAAPAML
                     GKSDVVSLARDVLNEFAQVGSSQTIEEHENHILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            4783..4968
                     /locus_tag="WJL87_000926"
     CDS             4783..4968
                     /locus_tag="WJL87_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ7799368.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            4979..5647
                     /locus_tag="WJL87_000927"
     CDS             4979..5647
                     /locus_tag="WJL87_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ7799369.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLMRLREKTGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEVGVFTGYSSTALALALPEHGRITACDINVSFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIVQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDPRIVPITLPVGDGLTL
                     LLKK"
     gene            5647..6363
                     /locus_tag="WJL87_000928"
     CDS             5647..6363
                     /locus_tag="WJL87_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ7799370.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRLEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGTLVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAMFKIGECQYRLQQKDIARATWRSL
                     IQAYPSSPAAKRAAAAVRKR"
     gene            6412..6897
                     /gene="purE"
                     /locus_tag="WJL87_000929"
     CDS             6412..6897
                     /gene="purE"
                     /locus_tag="WJL87_000929"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ7799371.1"
                     /translation="MTQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANENPELAQKLADFRAKQEQAVLSMELEQ
                     I"
     gene            complement(7080..8534)
                     /locus_tag="WJL87_000930"
     CDS             complement(7080..8534)
                     /locus_tag="WJL87_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ7799372.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPTEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPILGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(8531..9232)
                     /locus_tag="WJL87_000931"
     CDS             complement(8531..9232)
                     /locus_tag="WJL87_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ7799373.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREMGVSWGSEVERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            complement(9229..10008)
                     /locus_tag="WJL87_000932"
     CDS             complement(9229..10008)
                     /locus_tag="WJL87_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ7799374.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVARAQLDLFPEEWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPNMPRPKHIASFLLERTGGKA"
     gene            10292..11698
                     /locus_tag="WJL87_000933"
     CDS             10292..11698
                     /locus_tag="WJL87_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7799375.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADL
                     NESPLNMQLAVISYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSPLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGANLTRNTPS"
     gene            11810..13336
                     /locus_tag="WJL87_000934"
     CDS             11810..13336
                     /locus_tag="WJL87_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ7799376.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPKYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIEGLL
                     "
BASE COUNT         2708 a         3607 c         3760 g         3271 t
ORIGIN      
        1 tgcgtgggaa tgacggtcgg gtatcagcgg aagaagtcgc ccactccggg gggtagacct
       61 tgcgtgaatg cgcccattgt tttgtttgcg gtttcttcgg ctttgccccg ggcggatttg
      121 agggcggcga ggatgaggtc ttcgagcatt tctttgtcgt cggcggcttc ttgaatcaaa
      181 tcggggctga tgtcgatttt gcgtacttcg tgcgcgcagg tcattgtgat tttgaccagg
      241 ccgttgcctg cttcgccttc gatttcggtt tcggcaagtt tggcttgcgc ttttttcata
      301 ttttcctgca tttgctgcgc ctgtttcatc aggccgccta atccggcttt tccgaacata
      361 ctgaatactc ctgtctgttt tgattgaatg agggaaacgg caatgccgtc cgaagggttc
      421 gggcggcatt atatctgttt atggccggtt tgctgccaat tccagtgatt cgggctgcca
      481 ttgcgcgccg aatgcttgga ggattttttg tgcggcgggg tcggcttcga gcaatgcttg
      541 tgctttttgc ctgtcttcgg cttggacgcg cttgtcctgc atcgcggggg tttccctgcc
      601 ggcttcgtcg cgccagtctt gggtttgcag ggtgagttgc agtccgtagg cttgggcaag
      661 ggtgtcgcgg attttgtcga gacgtttttt gtcggcggtg gcgcgggctt cggcggtcat
      721 cgccaaaacc atcaggccgg tgtcgggatg gtattccgtc cacgcggagt gttgcgccgg
      781 catttgcgcc gcgccgagtt tgcgggcgaa atgccggacg atggctgccc agttttcggt
      841 ggaaaattcg ggcggcgggg cggacggcgt gttgtttccg ccgatgccgc ctgcttctgc
      901 ttcttcgtcc gcgcctccgc ctgcggtatc ggcaggggcg gcgtgttccc aatcgggcgg
      961 cgggatttct gcgccgtctt ctacggggca gtcattatcc ggaaagccgt aaccgtagaa
     1021 aggttctgca ggggcttcgt gtgcaaatgt ttccgtttca acggcttcat cattcggtgt
     1081 tgcctgaatg gggttttcag acggcacttc ggacaagggg gcatcggtta cgttgtcggc
     1141 tgccttgttc ttggaaactt ggttttcggg cggcgtttcg gcttcggatg ccgtctgaat
     1201 gctttttgcc gacgtttgcg ccgtgccggc tgcggcttgg gcttcgtctg gcgcgtcttc
     1261 ccaaggcgga acatcgttgt tttcttgatt ggaaacgggt ccggcagttt tgccttcaga
     1321 cggcattgcc gctgcggatg ccgtctgaac gggtgtttgg gcggtttctg cttcagggcg
     1381 cggttggggc ttttttacgg cggtttcctt ttcggcggtt tgtgccgatg gggattttag
     1441 ttcggtattt tcaatcacgg cgtttgtatc acacgaagct gccgccaagg gcgcaaacgc
     1501 cagcatacgc agcagggtca tcataaagcc ggcgtattcg tcgggggcga ggctcaggtc
     1561 gcgtttgccg tggacggcga tttggtagta aagctggatt tgttcgccgc ttatggtttg
     1621 ggcgaggcgg tgcaaaatat cggaatcggg gtcgtcgtgc gccaaggcgg agggcaccgc
     1681 ctgtatcagg gcgaggtgtt gcagcagtat ggcaagttcg cccaaggcgt tgtcaaagcc
     1741 gacggcacac gccgccattt cctgcgcttt ggcggtcagg gctgcgccgt cttggttgat
     1801 gatgcctgtc aagagttcgt aaaggtattg tttgtcaacc gcaccgatca tttggcggac
     1861 gtcttgttcg gcaacgctgc ccgaacccat ggcgatggct tggtcgagca ggctcaaggc
     1921 atcgcgcatc gatccggcgg cggcgcgtcc caaaagttgc agggcagggg gttcgtaggc
     1981 gattttttcg ctgtcgagga cgtgggcgag gtggtcggca acctgctgcg aagtcatatt
     2041 gtgcaagaca aattgcaggc agcggctcaa gacggtaacg gggactttat gcggatcggt
     2101 cgtggcaagg atgaatttga cgtgttcggg cggctcttcc agcgttttga gcatagcgtt
     2161 gaacgcgctt ttggaaagca tatgcacttc gtcgatgata tagactttgt atttgccggc
     2221 agtcggtgca tattgggcgt tttccaatac ttcgcggatg ttgtcgatgc ctgtgttgga
     2281 ggcggcgtcg atttcgagca ggtcgacata gcgtccggca tcgatttggg tgcagctttc
     2341 gcacacgccg caaggttcgc cgtgttgcgc gttttcgcag ttgaggcttt tggcaaggat
     2401 gcgggcgatg gtggttttgc ctacgccgcg cgtaccggtc agcaggtagg cgtggtgcag
     2461 cctgccttcg tccaaggcgt tttgcaaggc tttgacgacg tgttcctgac cgactaagtc
     2521 ggaaaaggtt ttgggccgcc attttcgggc gagaacttga taggccatgt ttttctcttg
     2581 gtttcggtcg tgatgtttct gtcggtgcgt cggaatgccg tctgaacggc ggcttcgggc
     2641 ggcgtattct agcactttcg gtttattgtc cgcgcaagaa cagtgcgtcc aattctttca
     2701 aagtcagttt gacccaagtc ggcctgccgt ggttgcactg gttgctgcgc ggcgtatttt
     2761 ccatatcgcg cagaagggcg ttcatttcgg gcagggtgag ccggcggccg gcgcggatcg
     2821 agccgtggca ggacatggtg gcgaggatgt ggttttcgtg ttcctcgatg gtttggctgc
     2881 tgccgacttg ggcgaattcg tttaatacgt cgcgggcgag ggagacgaca tcggatttgc
     2941 ccaacattgc gggtgcggcg cggacggcga gggtgttgcc gcccatatcg gacagttcca
     3001 gcccgaagcc tgccagtgct tcggcgtggt cggcaagggc ggcgcattct tcgtgggacg
     3061 cggcaaaggt tacgggaata agcaggcgtt ggctttgcag gttgccgttt tcctgacgtt
     3121 ggcgtttcat tttttcgtag ttgacgcgtt cggcggcggc gtgcatatcg atgagcaaca
     3181 ggctgtcttc ggcttgggca agaatgtaga tgccaagtaa ttgggcaatg gcgaaaccga
     3241 gcggcggcag ttcggatggg gacgggatgc cgtctgaaag cggcgtatct gtttggggag
     3301 caggcgtttc agacggcata ttgccgaaac gtgcctgctc gaatcggctt aactcaaggt
     3361 cgatgtcgtc ggtttttttg taaagttcgg cgtaagtatt cattgccgcg cggctttcgc
     3421 gcagggacag gctgcgttgt tgcggcgcat atgcggactg atagggcatg ggcgcggttt
     3481 tgcctgatga accaaaggca ttgtgtgtat ctgatttgtt gcctgtcggg tagttggatg
     3541 cgcgatcaaa cagattttcg ctgttgtttt cagacggcat tggtgcaggc gtaacgccgg
     3601 taatgtcatg caacacttcg cctgcgttgc tgacgctttc ggtcaggtcg gcgcgtgtgt
     3661 cggcaagggc tttgttgagc gtgtggaaca caagttggtg cacctgctga ctgtcgcgga
     3721 agcggatttc ggttttggtc gggtggacgt tgacatccac ggcttcgggc ggcaggtcga
     3781 gaaagaggac gaaagcaggc gtcagtgcgt tgtgcaatac gtcgcggtat gcctgcttga
     3841 cggcatggag catgactttg tcgcggacaa aacgatggtt gacgaagcag tattgtttgt
     3901 cggttttacc tttggcgaaa gtcggtttgg caatcgcgcc atagagccgc aatgcgccgt
     3961 tgtcgctgtc aatttccaat gatgccgtct gaaaatcatc gccgacaatg gcggcaatcc
     4021 gttcatgcag gctttgtgca gggagtttga acacttgttt gccgtcgcgt ttgagcgaga
     4081 aggcaatgtg cggatgcgcc agcgcgaggc gttcgagcat agtggcgcag tgggcgtatt
     4141 cggtggcttc agacttgagg aacttgcgcc gcgcaggggt attgaagaag agttcggcgg
     4201 cttcgatggt ggtgccgacg gggtgggcgg cggcggtggg gctgctgagt ttgccgtctt
     4261 cggctttgac ttgggttgcg tgcgaactgt cttcttgacg gctggtcagg gtcaggcggc
     4321 tgacggaggc gatgcttgcc aagccttcgc cgcgaaagcc catgctggca acgtgttcca
     4381 aatcgtttaa ggttttgatt ttgctggtag cgtgtcggtg gagcgcaagc tcgatgtcgt
     4441 cggggtggat accgccgccg ttgtcgctga cgcgaatcag gcggatgccg ccgcccgcca
     4501 gctcgacatc aatcgccgtt gcgcctgcat cgatactgtt ttcaacaatt tctttcaagg
     4561 cgttggcagg gcgttcgact acttcgccgg cagcgatttg gttgacaaga tggtcgggca
     4621 gggcggcaat tcgggacatg gcatgggctt ccgtttcaaa aaacgcttta ttctataata
     4681 aaacccctta tctttctgcc cgtattcttg ataaaagccg tttatcccgt ttggaaaata
     4741 ccagtataat cacgccgtat cccgtaaaat tggagcgcaa agatgtacca ctaccaatcc
     4801 gaagccacac aattcctcaa ccgcctgatt gaagaaaaac ctgagttgga acagcagcgt
     4861 ttggaaaacc gggggctttt gtgggatgtc gaactcaatc ccgaagaaca ggaaaacttc
     4921 gaggcggcaa aagtagcgaa aaaaccttat acctattacc aagactgaac ccgccgaaat
     4981 gacgacccat ctgtccaatg tcgcccccga cctgcaaaac tatttgaacg ccatcggcga
     5041 acctgaacat cccgttttga tgcggctgcg cgagaagacc gggcatcacc gtatgggcaa
     5101 aatggcgatt gcgcgcgaac aggcggcggt tttggtttgg ctggcaaagc tgatccgtgc
     5161 ggaaaaatat ctggaagtcg gcgtgtttac tggatacagc agcaccgcgc ttgcattggc
     5221 gttgcccgaa cacgggcgga ttaccgcctg cgacatcaat gtaagcttta ccgatacggc
     5281 gcgtcaggtt tggaacgagg caggtgtggc acataaaatc agcctgcacc tgcaacccgc
     5341 attgctgaca ttggatgatt tgattgtaca gggtgaagcc ggaagctacg atttggcact
     5401 gatagacgca gacaaaccgc ccacgccgca atatttcgag cgttgcctca aactcgtccg
     5461 gcaaggcggc atcattgcca ttgacaatat tttgttgaac ggaagggtga tgcgcgaagc
     5521 ggcttccgac gcgccgccca gtgtcggcat cctcaaagat ttcaatcaaa acctgccgaa
     5581 cgacccgcgc atcgtcccca tcaccctgcc cgtcggcgac ggtttgaccc tgcttctgaa
     5641 aaaataatga agaccaaatt accgcttttt atcatttggc tgtccgtatc cgccgcctgt
     5701 tcttcccctg tttcccgcaa tattcaggat atgcggctcg aaccgcaggc agaggcaggt
     5761 agttcggacg ctattcccta tcccgttccc actctgcaag accgtttgga ttatctggaa
     5821 ggcacactcg tccgcctgtc gaacgaagtg gaaaccttaa acggcaaagt caaagcactg
     5881 gagcatgcga aaacacaccc ttccagcagg gcatacgtcc aaaaactcga cgaccgcaag
     5941 ttgaaagagc attacctcaa taccgaaggc ggcagcgcat ccgcacatac cgtcgaaacc
     6001 gcacaaaacc tctacaatca ggcactcaaa cactataaaa gcggcaggtt ttctgccgct
     6061 gcctccctgt tgaaaggcgc ggacggaggc gacggcggca gcatcgcgca acgcagtatg
     6121 tacctgttgc tgcaaagcag ggcgcgtatg ggcaactgcg aatccgtcat cgaaatcgga
     6181 gggcgttacg ccaaccgttt caaagacagc ccaaccgcgc ccgaagccat gttcaaaatc
     6241 ggcgaatgcc aatacaggtt gcagcagaaa gacattgcaa gggcaacttg gcgcagcctg
     6301 atacaggctt acccgagcag cccggcggca aaacgcgccg ccgcagccgt acgcaaacga
     6361 tagtttttcc atttttccgc tttttacccc atccgcacca aggagagatt tatgacacaa
     6421 atcggcatca tcatgggcag caacagcgat tggcccgtta tgcggcaggc agcgcagttt
     6481 cttgaagagt tcggcgtaga atatgaggcg cgcgtcgttt ccgcacaccg cactccggat
     6541 ttaatgttcc aatacgccga aaccgcacgg gcgcgcggca tcaaagccat catcgcgggt
     6601 gcgggcggcg cggcgcattt acccggcatg gttgccgcca agaccaccgt ccccgttttg
     6661 ggcgtacccg tccccagcaa atacctgcgc ggcgaagatt cgcttttatc gattgtacaa
     6721 atgcccaaag gcgtacccgt cgccacattc gccatcggcg aggcaggcgc ggcaaatgcc
     6781 gcattgttcg ccgtttccat gctggccaac gaaaaccccg aactggcgca aaaactggca
     6841 gacttccgag ccaaacagga acaggctgtt ttgagtatgg aattggaaca gatttaaaac
     6901 ccgccgtccg cgaatatata gtggcttaaa tttaaaccag tacggcgtta cctcgccttg
     6961 ccgtactatt tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta
     7021 tacaaatgcc gtctgaagcc ctgtttcggg tttcagacgg catttttgca aacaactgtt
     7081 tatgcctgtt ctttttggcg cattttttct gccattagtt cgtgcagggt tttcttcgcc
     7141 ggtttcatcg gcacgcggtt ttgcgtccaa cccaactgtt tgcgcggggt caggttgcgg
     7201 aacttggtgg ctgcccaacc gaaggcgcgg taggttttgc tgccgctgaa aataccgttg
     7261 aatgtgcgcc acgccatttg ttcgccgaag gtatgcgatg cgccttgccc taggatgggg
     7321 tgtggcacgg tttcggtcgg cgaacgttgc gcttcaacgc gcaaacgctg catttgttcg
     7381 gtaatcggga tgcgtaccgg acagacttcc acacacgcgc cgcacatggt acaggcggtt
     7441 ggcaagtcgc gggtggcatc cagacccaac aggtgagggg aaataatctc gccaatcggg
     7501 ccgggatagg ttgtgccgta tgccgcgccg ccgatacggg tataaaccgg gcaatggttc
     7561 atacacgcgc cgcaacggat acattgcaag gtgcggcgca tttggtcttc ggcataggct
     7621 tggctgcgac cgttgtcaag caggaccaag tgcatttctt gaggaccgtc cagctcttcg
     7681 ctacggcgcg ggccggtaat catattgaaa taagtggtaa tgttctgacc aatggcagaa
     7741 cgcggcagca ggctgtacaa gggtgggatg tcggacaatt tcgccaccac tttttcaatg
     7801 ccggtaatag cgatatgcac gggcggtacg gtggtactca agcgaccgtt gccttcgttt
     7861 tccaccagac acagcgtacc cgtttcggca acagcaaagt ttacgccact caaaccgaca
     7921 tcggcagtgc tgtaaatatc gcgcagggct ttgcgggcga agccggtcag ttggtccacg
     7981 tcgtccgtaa gcggagttcc aaggttttgg tggaacagtt cactcacctg ttctttggtt
     8041 ttgtgaatgg cgggcatcac gatatgggtc ggtttttcgc ctgccatttg gacgatgaac
     8101 tcgcccaagt cgctttctac cgctttaatg ccttttgctt caagataatg gttcagctcg
     8161 atttcctcgc tgaccatcga tttgcctttg accatcagct tgccgttttt ggctgtgatg
     8221 atgtcgtgga taatttggca ggcttcggtc ggggtttctg cccagtgcac tttcacgccc
     8281 aacttagtca ggttttcttc cagctgctcc agcagggctg gcaatttaga caatgagcgc
     8341 tgacggacgt gttcgcacaa atcgcgcagg ctttgcagct cttcttcgtc ggtcaaaacg
     8401 gctttgcgtt tggtcatcag catatccatc gcggtacgca ggcttttgcg caaaggcttg
     8461 tcttgaaggg aaattgcggc gttttgcttg aaagtttccg gcttcatgtg aaatttgatg
     8521 gtttgcgtag tcatgcgttt tcctccaaat cggcagggga aatgtggtcg ggcaggatgg
     8581 cgaggatgac caaatcgcgc gggccgtgcg cgccgtaagc aagcgtcagt tggatgtctg
     8641 cggttttgga cgggccggaa atcaggaata cattggtcgg cataccgttt tccaccagtt
     8701 tttcgccttc gacggcatta tgaaactcgt tgtacatctt ggacgtatcg aacaggcaga
     8761 aatgcacggg cggaacgaga ctcaaagtac gcggttcttc ggggctggaa aacagcatca
     8821 gcgtgccggt gcgggcgatg ccgcattgcg cgccgctgaa gcccgcatcg atgttcgtga
     8881 aaaactcggt tttccaagta tcgatttcgc gctcgaaggc aatcggttcg atattgctgt
     8941 ccgccaatgc ggcacgggca atttgtccgt gttcggtcgc caagggcagc aggatgtttt
     9001 tcaaaccctt gccttctgcc gcttcgcgga aaacctgcat ccaattgctt ttcgtcaccc
     9061 aataaatttc ggttttgacc gcacgcatag cggcagccca atgtttcaga cgctcaactt
     9121 cgctgcccca agaaacaccc atttcacggt aataatcaaa aaccgcaggt tcttccatcg
     9181 gcaatgcgtc ggcttttttc agttttgcca aaatattttc gcgcgcgctc atgctttgcc
     9241 tccggtacgt tccaacaaga aggatgcgat atgtttcgga cgcggcatat tcggttcgtc
     9301 cttagcaatt ttgccgccga tgttcatcat acagccgcag tccgcgctga tgatttcggt
     9361 tgcgccggtt tctttcagcg cggcgacttt gtcggttacc atcgcgccgg aaatatcggc
     9421 ttgtttgacg gagaatgtgc cgccgaagcc gcaacattcg ctttcgtggt cgtggacgat
     9481 gcgttcgacg ttttccatac cgtcaatcag ttgccagccc gagagatgga cattcatttc
     9541 gcggcgggcg gcgcaggaag tgtgaacggc gactttgagc ggttcgccct tgtcttcggg
     9601 tttgaaaccg atggcaagca ggaaatgggt aaactcgatg atgcggccgg cgcaatccac
     9661 agccctttcc tcgtactcgc tgcctttaaa cagcgtcggc caatggtgct tcatcatacc
     9721 gccgcacgaa ccggacggta cgacaatcgg ccactcctcg gggaaaaggt cgagttgcgc
     9781 tctggcgaca tcaaaggctt cggtcggatg gccggatgaa taggcgggct gaccgcagca
     9841 gctttgcgcc atcgggaaat ggacgcgtat gccctgttgc tcgattaggg taatggcatc
     9901 catgcctgct tcgggcataa aaagatcaag gacgcaagtg ccgaaaaaat agacatcggt
     9961 cggattgctg tcgtatcgga tgatttttgg gggaatggtt gcgctcatgt tttttgataa
    10021 cgggaaaccc gtttttttct gtagaaaggt aagcgtttac tttaagtaat tgactgttgc
    10081 gggtcaagtc taattttaaa aaataatccg gtttttctta caaactaccc cataacgctt
    10141 actgtacctt aatctgatag ttttcgataa taattatcat tacaatgcaa tgccggttcg
    10201 tttgcttgtg aacattcaag atgccgactc tgacggcatt cagacagcat ctgaaaacaa
    10261 taacggcaca aggatggggt acaatcgctc tatggaaaaa aacaccctat ccgcccgcgc
    10321 accatcccct tggctgccgc ttctgctggc aattgccatt tttatgcaga tgttggatgc
    10381 gaccatttta aataccgcac tgcctgaaat tgccgccgac ctgaatgagt cgccattgaa
    10441 catgcagctg gcggttattt cctacacgct gacggttgcc ctgctgattc ctttgagcgg
    10501 ttatttggcg gacaggttcg gaacgaaaaa agtctttttc ggttcgattg ccgtttttat
    10561 gctcggatcg gcattgtgcg ccgcatcggg ttcgctgttt gaattgacgc tttcccgtgt
    10621 cgttcagggc atcggcggtt cgatgctggt tccgataccg cgtctgacca tcttgcgtgt
    10681 gtacgacaag tccaagctgc tcaacgccat caattatgcg gttatgcccg cattaatcgg
    10741 accggtttta gggcctttgg cgggcggtta tctggtcgaa tacgcttcgt ggcactggat
    10801 tttcctgctc aacctgccca tcggtcttct gggtttcata ttgggacgca acatcatgcc
    10861 cgatattaaa ggcagcaata tctctttaga cttcaaaggc tatctgattt tttctgccgc
    10921 cgcgtgcctc ttgttacttt cggcagaaag cctgtcgcac gcgctgcctc cgtattttgc
    10981 actgttgccg ctgtgcggcg gactgctgtt tgcacgccgt tatttccgac atatgaaaac
    11041 cgcgtccaaa ccgatttatt ccgccgacct gtttctgata cgcactttcc gtctaggact
    11101 ggcgggcaat ctgttcagcc gtctcggcat cagctcgatt ccttttctga tgcccctgat
    11161 gtttcaagtc gctttcggct tcggcgcaag cctgtcgggt tggctggtcg cccccgtcgc
    11221 cctgtcttcg ctgctggtca aaccgctgat tgcaccgctc atgaaacgtt tcggctaccg
    11281 cacggtactg ctttggaaca ccaagctgct tgccgccttc atcatgctgc tcgccctacc
    11341 tgatggaaac tcgccgctgt ggatttggat tttcctctcg ctggcgatcg gcgcgtgcaa
    11401 ctccctacag ttttctgcca tgaacacact gaccctcgcc gatttgcgcc cgcaacagac
    11461 cggcagcggc aacagcctga tggcggtcaa ccaacagctt gccatcagca tgggcattgt
    11521 tgtcggcgca ttaatcctta aaaactggac atttctgata ccggcttctt caggtctgca
    11581 ttccgccttc cgtatgaccc tgctcagtat cggcggcatt acccttgcct cgtcgttggt
    11641 tttcaagcgt ctgcacgttt cagacggtgc aaacctgaca cggaacacac cgtcctaaag
    11701 cggtccgcac gcaaaacttt tacccgtttc aacgtttgga ttatgattcc gcacttccat
    11761 gcgcaccaac cccaaaacac aggcaatgcc gtctgaaacc atatccctga tgaaaacacg
    11821 cagcctaatt tcccttttat gcctccttct ctgttcatgt tcttcatggt tgcccccact
    11881 ggaagaacgg acggaaagcc gtcatttcaa tacttccaaa cccgtccgcc tggacaacat
    11941 cctgcaaatc cggcacaccc ctcataccaa cgggctatcc gatatctatc tgttgaacga
    12001 cccccacgaa gcctttgccg cccgcgccgc ccttatcgaa tctgccgaac acagcctcga
    12061 tttgcaatac tacatctggc gcaacgacat ttccggccga ctgctgttca acctcgtgta
    12121 ccttgccgca gaacgcggtg tgcgcgtacg cctgctgttg gacgacaaca acacgcgcgg
    12181 attggacgac ctcctgctcg ccctcgacag ccatcccaat atcgaagtgc gcctgttcaa
    12241 ccccttcgtc ttacgaaaat ggcgcgcact cggctacctg accgacttcc cccgcctcaa
    12301 ccgccgcatg cacaacaaat cctttaccgc cgacaaccgc gccaccatac tcggcggacg
    12361 caatatcggc gacgaatact tcaaagtcgg tgaggacacc gttttcgccg acctggacat
    12421 cctcgccacc ggcagcgtcg tcggcgaagt atcgcacgac ttcgaccgct actgggcaag
    12481 ccattccgcc cacaacgcca cgcgcatcat ccgcagcggc aacatcggca agggtcttca
    12541 agcactcgga tacaacgacg aaacgtccag acacgcgctc ctgcgctacc gcgaaaccgt
    12601 cgaacagtcg cccctctacc aaaaaataca gacaggacgc atcgactggc agagcgtcca
    12661 aacccgcctc atcagcgacg accctgcaaa aggactcgac cgcgaccgcc gcaaaccgcc
    12721 gattgccggg cggctgcaag acgcgctcaa acagcccgaa aaaagcgtct atctggtttc
    12781 accctatttc gtccccacaa aatccggcac agacgcactg gcaaaactgg tgcaggacgg
    12841 catagacgtt accgtcctga ccaactcgct acaggcgacc gacgttgccg ccgtccattc
    12901 cggctatgtc aaataccgaa aaccgctgct caaagccggc atcaaactct acgagctgca
    12961 acccaaccat gccgtccctg ccacaaaaga caaaggcctg accggcagct ccgtaaccag
    13021 cctgcatgcc aaaaccttca ttgtggacgg caaacgcatc ttcatcggct cattcaacct
    13081 cgacccccgt tccgcacggc tcaatactga aatgggcgtt gttatcgaaa gccccaaaat
    13141 cgcagaacag atggagcgca cccttgccga taccacaccc aaatacgcct accgcgttac
    13201 cctcgacagg cacaaccgcc tgcaatggca cgatcccgcc acccgaaaaa cctacccgaa
    13261 cgaacccgaa gccaaacttt ggaaacgcat cgccgcaaaa atcctatccc tgctgcccat
    13321 agaaggttta ttatagaaat gtagcg
//
LOCUS       DAWKST010000048        13240 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.048, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000048 DAWKST010000000
VERSION     DAWKST010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13240
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.048"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(44..811)
                     /locus_tag="WJL87_000935"
     CDS             complement(44..811)
                     /locus_tag="WJL87_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ7799377.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGALTALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVYETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(899..1726)
                     /gene="mutM"
                     /locus_tag="WJL87_000936"
     CDS             complement(899..1726)
                     /gene="mutM"
                     /locus_tag="WJL87_000936"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ7799378.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCADYLYVRLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLRRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            complement(1778..2443)
                     /locus_tag="WJL87_000937"
     CDS             complement(1778..2443)
                     /locus_tag="WJL87_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEZ7799379.1"
                     /translation="MDSWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGVPSQILSEAHRILKPSGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRQLADVGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     IAD"
     gene            2622..4598
                     /locus_tag="WJL87_000938"
     CDS             2622..4598
                     /locus_tag="WJL87_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ7799380.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            4666..5358
                     /locus_tag="WJL87_000939"
     CDS             4666..5358
                     /locus_tag="WJL87_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEZ7799381.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMIAAVGNRVQHLHRRRFAHLETENLK
                     PGEWKFIDCPKF"
     gene            5902..6345
                     /locus_tag="WJL87_000940"
     CDS             5902..6345
                     /locus_tag="WJL87_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ7799382.1"
                     /translation="MTVAGATVGFGATWRFPYLVGENGGGAYVFLFCIAMLVIGIPMI
                     LVENVIGRRKGVNALDAFGGPMNGKPIAKIWKLVGWMGLLGAFGIMAYYMALGGWVIS
                     YIVNIIGGNLNISSPIDGVVTKGFFAEHIETALGKLRFIRCFLSP"
     gene            6405..7214
                     /locus_tag="WJL87_000941"
     CDS             6405..7214
                     /locus_tag="WJL87_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ7799383.1"
                     /translation="MPLLFLFLIAMVVRNVTLPGAMEGVAFYLKPDFSKITAELFVFV
                     LGQVFFALSLGFGVMITLSSYLDKNENLVQTAVITAITNTIIAILAGFMIFPSLFSFG
                     VAPDSGPTLVFQSLPIVFSHMWAGSVFAVIFFSLLLIAALTTSLTIYEVLITTIQEKT
                     KIRRTAAITIVLAAIFIFGNIPSILSYGPWKDVSIFGKNIFDAFDYISGNILFMLTAL
                     GSALFVGFVMKDEAKEELLYKGNHTTVNIWFAYVKYLVPLVILLIFISNLF"
     gene            7458..8114
                     /locus_tag="WJL87_000942"
     CDS             7458..8114
                     /locus_tag="WJL87_000942"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ7799384.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSGSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYREV
                     "
     gene            8270..9955
                     /gene="rpsA"
                     /locus_tag="WJL87_000943"
     CDS             8270..9955
                     /gene="rpsA"
                     /locus_tag="WJL87_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ7799385.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGDILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            9966..10280
                     /locus_tag="WJL87_000944"
     CDS             9966..10280
                     /locus_tag="WJL87_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ7799386.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(10630..11037)
                     /locus_tag="WJL87_000945"
     CDS             complement(10630..11037)
                     /locus_tag="WJL87_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ7799387.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            11185..12294
                     /locus_tag="WJL87_000946"
     CDS             11185..12294
                     /locus_tag="WJL87_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ7799388.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINKAFDLMHEGKSIRAVIHY"
     gene            12303..13130
                     /gene="fghA"
                     /locus_tag="WJL87_000947"
     CDS             12303..13130
                     /gene="fghA"
                     /locus_tag="WJL87_000947"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ7799389.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVASDTSPRGEQVPNDDAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
BASE COUNT         3510 a         3635 c         3046 g         3049 t
ORIGIN      
        1 atccgcaaaa gcgaatcata ctgccacatt ccggcaaccg gtttcagaca gcgatatccg
       61 catcgtcggc gacaacggca cggatgcttt cttcgatttt atcttttaaa gcataacggt
      121 cttcgctttc cgccgcatcc gccacgcaaa cgaaatcgac ttttatcgtt aattttttca
      181 tagacacaat gcgccacagg caggtcggca aaccgacatc ggcatatgag ggacgagccg
      241 tcctttttcc cgtttcgtca taataacgca gcgcgaccgc caaaaccttt gcccccgcat
      301 caatggcgga ttggaacagc gcggctttga acggcaggag ccccagtccg gaggaagtcc
      361 gcgcttcggg gaaaaaactg acgttttgac cgcgttgcaa ggtttcgtag acggcgcggt
      421 taatcggttc gatgtcgcgc cgcgaattgc ggttgatgaa caccgttccc gcgttctgcc
      481 ccatcttgcc caataccggc cagcttttga tttcctgctt ggcgataaag ctgctcggat
      541 aaaccgcgct catcgcgaaa atatccagcc aggacacgtg gttggcggca accaagacac
      601 cgttcggatg ttcgggtgcg ggcctgccca cctccaatcc gatatccaaa gccgtcagag
      661 cccccctgcc caactcgatt accgcccgat tgcgcgactc ggggcagccg ccgtcaatac
      721 cgcgcaggtt tttgccggtt ttgaatagcc agaccgtcaa gcggcacaag cggcgcagac
      781 gtgtaaaaaa tgaagcttta tttgaagaca tgaacaattc tttcccgata atcggtgttt
      841 gttgttgaaa cgttcggacg gcataccgat aaaatgccgt ctgaaaccgt tttccgccct
      901 atttctgaca attcgggcag taaaacgtgc cgcgctgccc caaagtttct ttcacaacca
      961 aaccgccgca ctgggggcac ggctgattgt gccgcccgta caccgtatat tcctgttgga
     1021 agtagccact tttgccgtcg ctgtccacaa aatccctcaa ggtactgccg cccgtttcaa
     1081 tggcgcgccg caacaccgct ttgacggttt caaccaaaag cgcacactct ttctttttca
     1141 gacggttggc aggacggtgg ggcgaaatgc ccgctctgaa caggctctcg ttggcataaa
     1201 tgttgcccac accgaccacg accgcattgt ccatcagggc aagtttgacc gcgcgtttct
     1261 gcgccttcag cctcacatac agataatccg cacaaaatgc ctccgacaaa ggctccggcc
     1321 ccagtttttc caacagcgga tgatgttctt cgattccctc ataccaaagt atcgcgccga
     1381 actttctcgg atcgcggtaa cgcatgaccg tgccgtctga aaacacaata tcgacgtgat
     1441 cgtgtctgtc cggcctgccg atacgtccgt ccgacggcgt aaaaatccgc aagctgcccg
     1501 acatccccaa gtgaatcagc aaaatccccg tttggaaacg gacaatcaga tatttggcac
     1561 gcctgccgca ggacaaaacc tgccgaccgg acaaaatctc ccccaaatcg ggattgatct
     1621 gccagcgcag cttcaattgg cgcaatacca cggcttccac cgttttccct tcaatatgcg
     1681 gcgcgatgcc gcgcaacgtc gtttccactt ccggcaattc cggcataacc cctcccgaca
     1741 tttcttctga cagatgccgt ctgaaagacg gctgtcccta atccgcaatc cttgccgcac
     1801 ccgccgcaag ggctttgccg cccaaatacc cgtcccacgc gggcaggggc gttacgcccg
     1861 ccgccctttt gaccaaaacc aaaccgtaca ctgcggcaca ctgcggccac caacggtcgc
     1921 ccgccttttc cataaaccgc caaaagcgta tttgcccgag cgacgaaacg ggcggcagat
     1981 acaccataaa tttcccaaat tcaatatcga aaccgacatc cgcaagctgc cttttcaact
     2041 cgggcagcgg cagacagaag cgtttttccg gcaggcgttc gccgtcaaac caacggctga
     2101 atccccagag cgaatacgga ttgaaacccg tcagcatcaa acgtccggac ggtttcaata
     2161 tccggtgcgc ctcggacagg atttgcgaag gaacaccgcc ttcaagcgta tgcggaaaaa
     2221 gcagcatatc cgcagaaaca tccgccaaag ccatattttc cgccgacatc gacatatctc
     2281 gcggcacaca gacaacatct tcagacaggc tcagccacgg accgcccacc tgaaccgcac
     2341 acattcccga aaaacggtat gaatctagat accgcccgaa gaaatcctgt tccaattttg
     2401 caacataccg ccccatcgcc gtatcttcaa accatgaatc catattgcgt ccgtttcaaa
     2461 cagattgcct gccgatattc tattccaaac aggatttctg tcaaaaaaca catcggccgc
     2521 ccatttccga atccgcataa agttcctgta aaacttgacg ctttttcagt caaacagtac
     2581 catcggacga taaaatatgt ttattccgcc gaatataaat catgtccaaa ctcaaaacca
     2641 tcgctctgac cgcatcaggt ctgtccgttt gtccgggttt cctatacgcc caaaacacct
     2701 catcacacca aatcggtttg gcgattatgc gcttaaactc ttcaatactc gacctgcccc
     2761 cgacaaaaca atatttccaa tccggcagcc tgtggagcga gctgcgccaa ggcttccgga
     2821 tgggcgaagt caatcccgaa ctggtacgcc gccacgaaag caaattcatc gcaagccaca
     2881 gctatttcaa cagggtcatc aaccggagta gaccctatat gtaccatatc gccaacgaag
     2941 tcaaaaaacg caatatgccc gccgaagccg ccctgcttcc cttcatcgaa agcgcgttcg
     3001 tcaccaaagc caaatcacac gtcggcgcat caggattatg gcagtttatg cccgctaccg
     3061 gcaggcatta cggcctggaa aaaacaccgg tttacgacgg caggcacgac gtttacgccg
     3121 ccaccgatgc cgcactcaac tatctgcaat acctctatgg actgttcggc gactggccgc
     3181 ttgcctttgc cgcctacaac tggggtgaag gcaacgtcgg acgcgccatc aaccgcgccc
     3241 gcgcccaagg gctcgaaccg acctacgaaa acctgcgtat gcccaacgaa acgcgcaact
     3301 atgtccccaa gctgctcgcc gtgcgcaaca ttattgccac tccccaatct ttcggcatga
     3361 atatcagcga catagacaac aaaccgtatt ttcaggcagt cgaaccggat cgtccgctcg
     3421 acaacgaagc catcgcccgg cttgccggca tcacgcaaag cgagctgctc gccctaaacc
     3481 ccgcattcaa cgtccccgcg tttatcccca aaagcaaacg caaactgctg cttcctgtcg
     3541 cgtccgtaca aaccttccaa agcaactacc tcaacgccgc acccgacagc ctgttttcat
     3601 gggaagtcta tacgcctgcc gccaaaacca gcctgtccga catctcgacg gcaaccggca
     3661 tgagcattgc cgacatcaaa cgcctcaaca acctgaacgg caaccttgtc aacgcaggac
     3721 gcagcatcct tgtcgccaag aacggcaaaa cccttcagac ggcatcggaa tccgtcgttt
     3781 ccatcgacat cgacaatacg cccgacacct accgttccaa tatgccggca ggcacggtga
     3841 acgtcggcat tgcccgaatc cgacccgccg ccgcacagac agcggacatt accgtcgcac
     3901 ctttgccgca gaaaaccgtc cgtacggaac ccgatcccct tgtccgtatt gccgaacctg
     3961 cccttgcgac agccgcagcg caacctcaaa ccgaaaaaca gaccgccatg ccgtctgaaa
     4021 cccaaaccgc aacactcgcg cagatcatcc cccaaaacga catgcaggcg gcagacgaac
     4081 tcatgcagct tgttgcccga aacaacctgc gccgacaggc tgaagaaacc atctccgccg
     4141 tcatcggcac gcctgacaca gttgccgaac acaacatttc ctcatctccg caacataccg
     4201 ttgccgccga cggcaaacgc cgggcacgtt tggaaacgcg cgtagccaaa gctgccgacg
     4261 gcgaagccga aatctccccg ctccatgcca gcatccaccg cgttgtagaa ggcgacaccc
     4321 tgttcaacat tgccaaacgc tacaacgtca gcgtagccga cctgattgtc gccaacaaca
     4381 tcaaaggcaa caccatccaa aaaggacagg tactccgcct ggcgcaggca gcccctgccc
     4441 aaacccgtat tgaaaaagta tcctacaccg cgcgcaaagg cgacaccttc aaaagtatcg
     4501 ccgcgcgctt caatatccat atcgacgaca tccgccggct caatcccaac ctgaacacca
     4561 tcaatccggg acagagggtc aaactgattg gaagctgatt cggatacggc acatgacagg
     4621 actttctcag tcctgtcttt ttcatcccac atttcccata ccatcatgaa actcatcaaa
     4681 tacctgcaat atcaaggcat aggaagccgc aagcagtgcc aatggctgat tgccggcggt
     4741 tatgttttca tcaacggaac ctgcatggac gacaccgatg cagacatcga ttcctcatct
     4801 gtcgaaacgt tggatattga cggggaagca gtaaccgtcg ttcccgaacc ctatttctac
     4861 atcatgctca acaagcctga agattacgaa acttcgcaca aacccaagca ctaccgcagc
     4921 gtattcagcc tgttccccga caatatgcgg aacatcgata tgcaggcggt cggcaggctg
     4981 gatgcggata cgaccggcgt attgctgatt accaacgacg gcaaactgaa ccacaacctg
     5041 acttcgccga gcagaaaaat tcccaagcta tacgaagtaa cgctcaaaca ccccacagga
     5101 gaaacgctct gcgaaacctt gaaaaacggc gtgctgctcc acgacgaaaa cgaaaccgtt
     5161 tgtgccgccg atgccgtttt ggaaaacccg accaccctgc tgctgaccat taccgaagga
     5221 aaataccacc aagtcaaacg catgatcgcc gccgtcggca accgcgtgca acaccttcat
     5281 cgtcggcgat tcgcacattt ggaaacagaa aacctgaaac ccggcgaatg gaaatttatc
     5341 gattgtccaa aattctgaaa caaaattcca taatttccct atattcaact atatgcttat
     5401 taaataagta gttttacaaa aattaactca aaaaaaaaca gttagcttgc gatataattc
     5461 gcttgtctga gtaacgcctg ttcaagcagg cttatgagta agacgttttc cccgtaatgt
     5521 gttttgccat ctatacttct cccccttgta tagatggttt ttttatcttc gtttactaca
     5581 catactacat atgttactga cgcagcattc ccgatcatat gacatatgaa attttatttc
     5641 accgtcccag tgtagaaaaa taaacgattc atctatctat aaaatctgat tcaatccttg
     5701 ttgatttatg caaatcagaa atatccacta taaaatataa ctctattagt gaaaataact
     5761 acacacttaa aaaacaatca gtcagttcct gttaaaaaac gcgtgtagaa tttccgcttt
     5821 atctcaatcg gacacggttt caatatgtct tccccttcaa ataccaatcg tcaaacctga
     5881 tccagccgat taacctatat cctgaccgtt gccggcgcga ctgtcggttt cggcgcgacg
     5941 tggcgtttcc cgtatttggt cggtgaaaac ggtggtggcg cgtatgtgtt tttattctgt
     6001 atcgcgatgc tggttatcgg cattccgatg attttggtgg aaaatgtcat cggacggcgc
     6061 aaaggcgtga acgcgctgga tgcgttcggc ggtccgatga acggcaaacc cattgctaaa
     6121 atttggaaac tggtcggctg gatggggctg ctcggcgcgt tcggcatcat ggcttattac
     6181 atggcactcg gcggctgggt aatcagctat atcgttaata ttattggagg aaatttgaat
     6241 atttccagcc ccatcgacgg tgtggttaca aaaggcttct ttgccgaaca cattgaaaca
     6301 gcccttggga aattgcgttt tatacgctgc tttttgtcgc cgtgaaccaa tggattttgg
     6361 tcaaaggcgt tatcggcggc attgaaaaag cggcaaaata gctgatgccg ctgctgtttt
     6421 tgttcctaat cgcgatggtc gtccgcaacg ttacccttcc gggcgcaatg gaaggggttg
     6481 ctttctatct gaaacctgat ttcagcaaga ttaccgccga actgttcgtc ttcgttttgg
     6541 ggcaggtatt ttttgccctg agcctgggtt tcggcgtgat gattaccttg tccagctatt
     6601 tggataaaaa cgaaaatcta gttcagacgg cagttatcac ggcaattacc aataccatca
     6661 tcgccatact tgcgggcttt atgattttcc cgtcgctctt cagcttcggc gttgcccccg
     6721 attccggccc gactttggtg ttccaaagct tgccgattgt gttctcacat atgtgggcgg
     6781 gatctgtgtt cgccgtgatt ttcttctcgc tgctcctgat tgccgcgttg acaacttcgc
     6841 tgaccattta tgaagtgttg attacgacca ttcaggaaaa aaccaaaatc cgccgtaccg
     6901 ccgcgattac gattgtattg gctgccatct tcattttcgg caacatcccg tccattttga
     6961 gctacggccc ttggaaagat gtttccatat ttggcaaaaa tatttttgac gcttttgact
     7021 acatcagcgg caacatcttg tttatgctga ccgcgctcgg ttccgcgctg tttgtcggtt
     7081 ttgtgatgaa ggacgaagcg aaggaagaat tgctttataa aggcaaccat acgacggtca
     7141 atatttggtt tgcctatgtg aaatacctcg tgccgttggt gattctgctg atttttatca
     7201 gcaacctgtt ctaatccgca gcaatcaatg ccgtctgaag gtcatacctc tttcagacgg
     7261 cattgcattg atgcccgcca caatacccgc aacgccggaa tccgaaccgg cttgccggct
     7321 tctgtcggat gtttccgggc aggcggcgtt ccgtctgctt agacaatcgt cctttaaaac
     7381 aggtagaatc cgccccaacg ggaaacacat ccttcagacg gcaaaaccca taccccaaac
     7441 catcaggaat cccccttatg aacagacaaa aagtcatcgc catcgacggc ccgggcgcat
     7501 cgggcaaagg cacggttgcc gcccgcgttg ccgccgcatt gggatacgat tatctcgata
     7561 ccggcgcact ctaccgcctg actgccctat atgcacaaaa acaaggcgtg ggatggcacg
     7621 atgaagaaaa cgtttccgaa ctggcaaaaa aactgcccgc cgtattttca ggcagccgca
     7681 tcctgctcgg cggcgaagac gtttcagacg gcatccggac agaagccatc ggcatgggcg
     7741 catccgcagt cgcacagttg cctaaagtcc gcgccgccct gctgcaacgc caacgcgatt
     7801 ttctgaccga aaaaggactg gttgccgacg gacgggacac cggatcggtc gtcttccccc
     7861 aagccgaact taaaatcttc ctgacggcag aatccaaaat ccgtgccgaa cgccgcgcca
     7921 aacaaatcgg catcccctgc gaaggtttgg cattcgagcg catcctgtcc gacatcgaag
     7981 ccagagacga ggcagaccga aaccgcaaag ttgcccccct gaaacaacag cccgatgccc
     8041 tgcttttgga cacaagccgc ctgactatag aagaaactgt aaaaaaagtg cttgattggt
     8101 atcgtgaagt ttaaattttc aggtataatc gcacaaatta cgtttcagac ggcataaaaa
     8161 tcccccatat gccgtctgaa accttatgta cccgtctgcc ctgccaagga cggcagatat
     8221 ccaccaaccc accccgcacc ccttggcggt gtaccgaaaa gagttatata tgtctatgga
     8281 aaattttgct cagctgttgg aagaaagctt taccctgcaa gaaatgaacc cgggtgaggt
     8341 gattaccgct gaagtagtgg caatcgacca aaacttcgtt accgtaaacg caggtctgaa
     8401 atcagaatcc ctgattgatg tagctgaatt caaaaacgct caaggcgaaa ttgaagttaa
     8461 agtcggcgac ttcgttaccg ttaccatcga atccgtcgaa aacggcttcg gcgaaaccaa
     8521 actgtcccgc gaaaaagcca aacgtgcagc cgattggatt gccctggaag aagccatgga
     8581 aaacggcgac atcctgtccg gcatcatcaa cggaaaagtc aaaggcggcc tgaccgttat
     8641 gattagcagc atccgcgcat tcctgccggg ttctttggtc gacgtacgtc ctgtaaaaga
     8701 cacttctcac ttcgaaggca aagagatcga attcaaagtg atcaaactgg acaaaaaacg
     8761 caacaacgtc gttgtttccc gccgcgccgt tctggaagcc actttgggtg aagaacgcaa
     8821 agccctgctg gaaaacctgc aagaaggctc cgtcatcaaa ggcatcgtta aaaacattac
     8881 cgattacggt gcattcgttg acttgggcgg catcgacggt ctgttgcaca tcaccgattt
     8941 ggcatggcgg cgcgtgaaac acccgagtga agtcttggaa gtcggtcagg aagttgaagc
     9001 caaagtattg aaattcgacc aagaaaaaca acgcgtttcc ttgggtatga aacaactggg
     9061 cgaagatcct tggagcggtc tgacccgccg ttatcctcaa ggcacccgcc tgttcggcaa
     9121 agtatccaac ctgaccgact acggcgcatt cgtcgaaatc gaacaaggca tcgaaggttt
     9181 ggtacacgtc tccgaaatgg actggaccaa caaaaacgta cacccgagca aagtcgtaca
     9241 actgggcgac gaagtcgaag tcatgatttt ggaaatcgac gaaggccgcc gccgtatctc
     9301 tttgggtatg aaacaatgcc aagccaatcc ttgggaagaa tttgccgcca accacaacaa
     9361 aggcgacaaa atctccggcg cggttaaatc cattaccgat ttcggcgtat tcgtcggcct
     9421 gcccggcggc atcgacggtt tggttcacct gtccgacctg tcctggaccg aatccggcga
     9481 agaagccgta cgcaaataca aaaaaggcga agaagtcgaa gccgtcgtat tggcaatcga
     9541 cgtggaaaaa gaacgcatct ccttgggtat caaacaactg gaaggcgatc cgttcggcaa
     9601 cttcatcagc gtgaacgaca aaggttcttt ggttaaaggt tccgtgaaat ctgttgacgc
     9661 caaaggtgct gttatcgccc tgtctgacga agtagaaggc tacctgcctg cttccgaatt
     9721 tgcagccgac cgcgttgaag atttgaccac caaactgaaa gaaggcgacg aagttgaagc
     9781 cgtcatcgtt accgttgacc gcaaaaaccg cagcatcaaa ctttccgtta aagccaaaga
     9841 tgccaaagaa agccgcgaag cactgaactc cgtcaatgcc gccgccaatg cgaatgccgg
     9901 caccaccagc ttgggcgacc tgctgaaagc caaactctcc ggcgaacaag aataaggttg
     9961 cagacatgac aaagtctgag ttaatggttc gtttggcaga agtgtttgcc gccaaaaacg
    10021 gcacgcatct tctggcaaaa gacgtagagt acagcgtaaa agtcttggtt gacaccatga
    10081 ctagatcgct tgcccgaggt caacgcatcg aaatccgcgg tttcggcagc ttcgatttga
    10141 accatcgtcc tgcccgcatc ggtcgcaatc ccaaaaccgg cgagcgtgtg gaagtacctg
    10201 aaaaacatgt accccacttc aagcccggta aagaattgcg cgagcgggtc gacttggctt
    10261 taaaagaaaa tgccaattaa accttagcat caaaacgccg ctgttacgcg gcgttttttc
    10321 tgtggtttaa cttcatccgc tgcttcaata ccttgagcca agcaagcaac ggattagagc
    10381 gtggattttt tatagtggat taacaaaaac cagtacggcg ttgcctcgcc ttagctcaaa
    10441 gagaacaatt ctctaaggtg ctgaagcacc aagtgaatcg attccgtact atttgcactg
    10501 tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctatatcatt gcttacaatc
    10561 cgctttttaa acaacaaatt tttgatttct attacgaaca ggacaaaaat cctgcttatt
    10621 gcactaaaac taagccgttt caggaatttg cggcaaattt acggctttta ccgaacctaa
    10681 tgctttcgct ttttggtaaa acgccaattt gtattcaagc aaatctaaat agcgttttaa
    10741 ttcggcaatt tgacacttca cattttctat ttgattttca aacaaggaaa ggcgttcttc
    10801 aatggtatcg tcgccaatga cggtacattc cgcaaagcgt ttgatgtctt ttaagctcat
    10861 tcccgtattt ttcaagcatt gcaataagcc caaccattgc aaatcgttat cggtaaaaca
    10921 gcggttaccg tattcatcac gtccgatatt gggcaacaaa ccttctttgt cgtaaaaacg
    10981 tagggtgtgg gcggagatgc ctattttttc ggcggctttg gcagtagtat aagtcatttt
    11041 ccctccttct aaacaaaaac agtaaaaaac acttgcttta gagttaactc taaagtgtaa
    11101 actgttgcta tgttgctcag gcaaggcaac tttgtcaatg aattaagagg aaagacaatg
    11161 gaaatgaaac aagccgattc aaccatcaaa tctcgtgcgg cggtggcatt cgcccctaac
    11221 caacccttac aaattgtgga aatcgacgta gaaatgccgc gtaaaggcga ggtgttaatc
    11281 cgcaataccc acactggcgt gtgccatact gatgcgttta cgttatcagg aagcgatcct
    11341 gaaggcgtat tccctgtggt gcttggacac gaaggtgcgg gtgtggtcgt tgctgtgggc
    11401 gaaggtgtgt caagcgtaaa accgggtgat cacgtgattc cgctttacac cgccgaatgt
    11461 ggcgaatgtg agttttgttg ttcaggtaaa accaacttgt gcgtctcagt gcgtgataca
    11521 caaggtaaag gcttaatgcc ggacggcacg acgcgttttt cttatcaagg tcagccgatt
    11581 tatcactata tgggctgttc gactttcagt gaatactccg ttgttgccga agtttcactg
    11641 gcgaaaatca accctgaagc caaccatgaa caagtatgtt tgctcggctg cggcgttacc
    11701 acaggtattg gtgcggtaca taatacggca aaagtgcaag aaggcgactc tgttgccgtg
    11761 tttggcttag gcgcgattgg tttggcggtg gtgcaaggtg cgcgtcaagc caaagccggc
    11821 cgcattatcg ccattgatac caatccatca aaatttgagt tggcaaaaca gtttggtgca
    11881 acggattgtt tgaacccgaa cgattacgat aaaccgatca aagatgtgtt gttagacatt
    11941 aataaatggg gcattgacca tacctttgaa tgtatcggca acgtaaacgt aatgcgtcag
    12001 gcattagaaa gtgcacatcg tggttggggt caatccatta tcatcggcgt agcaggtgca
    12061 ggacaagaaa tttcaacgcg tccgttccag ttggtaacag gtcgtgtttg gaaaggttca
    12121 gcatttggcg gtgtgaaagg tcgctctgaa ctgccgaaaa tggtggaaga ttcaatgaaa
    12181 ggcgacattg agttagaacc gtttgtaacc cacacaatga cactcgatca aatcaataaa
    12241 gcctttgact taatgcacga aggtaaatcg atccgcgccg ttattcacta ctaaggtatg
    12301 cgatgaaact gattgaacaa catcaaattt ttggcggttc gcaacaagtt tgggcgcatc
    12361 atgcccaaac gctgcaatgc gaaatgaaat ttgccgtcta tttgccaaat aatccagaaa
    12421 atcgaccgct tggtgtgatt tattggcttt ccggcttgac gtgtacggaa caaaatttca
    12481 ttaccaaatc aggctttcag cgttatgcgg cagaacatca agtaattgtg gtggcctccg
    12541 ataccagccc tcgcggagag caagtgccga acgatgatgc ttacgattta ggacagggtg
    12601 caggctttta tttgaatgcg accgaacagc cttgggcggc gaattatcaa atgtatgatt
    12661 acattttgaa cgagctgccc cgtctgattg agaaacactt tcctaccaac ggcaaacgtt
    12721 ccattatggg gcattcaatg ggcggacacg gcgcattggt attggcgctg cggaatcagg
    12781 aacgttatca aagtgtttct gccttttcgc ctattttatc gccaagcctc gtgccgtggg
    12841 gagaaaaagc ctttactgct tatttaggga aagaccgtga aaaatggcag caatatgatg
    12901 cgaactcact cattcaacaa ggctataaag tgcaaggtat gcgcatcgat caaggcttgg
    12961 aagatgagtt tttgccgaca caattgcgta ccgaagattt tatcgaaacc tgccgtgcgg
    13021 caaaccagcc ggtcgatgtg cgtttccata aaggctacga tcacagctat tacttcatcg
    13081 ccagttttat tggcgagcat attgcttatc acgccgcgtt tttgaagtaa accaaagagc
    13141 gttcagtgtt caaagcagtt ttgggatagc cggcacgagg gcggtaagaa gtgccggcat
    13201 aaacgtatgc cgtctgagcc ggaaggagcc gactctacgg
//
LOCUS       DAWKST010000049        13216 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.049, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000049 DAWKST010000000
VERSION     DAWKST010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13216
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.049"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(58..1368)
                     /locus_tag="WJL87_000948"
     CDS             complement(58..1368)
                     /locus_tag="WJL87_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ7799390.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            complement(1507..2685)
                     /gene="ftsZ"
                     /locus_tag="WJL87_000949"
     CDS             complement(1507..2685)
                     /gene="ftsZ"
                     /locus_tag="WJL87_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ7799391.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(2804..4048)
                     /gene="ftsA"
                     /locus_tag="WJL87_000950"
     CDS             complement(2804..4048)
                     /gene="ftsA"
                     /locus_tag="WJL87_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ7799392.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVTSCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            complement(4134..4862)
                     /locus_tag="WJL87_000951"
     CDS             complement(4134..4862)
                     /locus_tag="WJL87_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ7799393.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            complement(4852..5766)
                     /locus_tag="WJL87_000952"
     CDS             complement(4852..5766)
                     /locus_tag="WJL87_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ7799394.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRNDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAESCVRVDFLKDTDGKLYLLEINTLPGMTSH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            complement(5879..7288)
                     /locus_tag="WJL87_000953"
     CDS             complement(5879..7288)
                     /locus_tag="WJL87_000953"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ7799395.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            complement(7443..8510)
                     /gene="murG"
                     /locus_tag="WJL87_000954"
     CDS             complement(7443..8510)
                     /gene="murG"
                     /locus_tag="WJL87_000954"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ7799396.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPFTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVQAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(8514..9839)
                     /locus_tag="WJL87_000955"
     CDS             complement(8514..9839)
                     /locus_tag="WJL87_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ7799397.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(9965..11302)
                     /locus_tag="WJL87_000956"
     CDS             complement(9965..11302)
                     /locus_tag="WJL87_000956"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ7799398.1"
                     /translation="MTFQNQKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDVFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIATQDIPLQGLHN
                     ATNVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALVGKAKGVFLIGVDAPQIRRDLDGCDLN
                     MTDCATLEEAVQTAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIGAFKAL"
     gene            complement(11389..11985)
                     /locus_tag="WJL87_000957"
     CDS             complement(11389..11985)
                     /locus_tag="WJL87_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ7799399.1"
                     /translation="MKQTVKWLAAALIALGLNRAVWADDVSDFRENLQAAAQGNAAAQ
                     YNLGAMYYKGRGVRRDDAEAVRWYRQAAEQGLAQAQYNLGWMYANGRGVRQDDTEAVR
                     WYRQAAAQGVVQAQYNLGVIYAEGRGVRQDDVEAVRWFRQAAAQGVAQAQNNLGVMYA
                     ERRGVRQDRALAQEWFGKACQNGDQDGCDNDQRLKAGY"
     gene            complement(12111..13193)
                     /locus_tag="WJL87_000958"
     CDS             complement(12111..13193)
                     /locus_tag="WJL87_000958"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ7799400.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
BASE COUNT         3100 a         3812 c         3357 g         2947 t
ORIGIN      
        1 tggcaaacaa aatgccgtct gaaaaccttt cagacggcat ttttaatatc gaatcaatca
       61 gcccaaatac cagaatttca gtgcccacaa tacggcaaca atccatacca taggcggaac
      121 gtctttggtg cggcggcata aaagtttaac cacggcatag ctgatgaagc cgaaagcgat
      181 gccgtctgca atcgaataag taaacggcat gaaaacaatg gtcaggaacg caggtgcggc
      241 ttccgtcata tcgtcccaat caatatcccg ggcactgcgg agcatttgtg tgccgacata
      301 aagcagggcg ggcgcggtgg caaaagcggg aacacttttc gccaaaggtg aaaacatcag
      361 gcaggcgagc atcaatacgc cgacggtaac cgccgtcagg ccggtccgtc cgcctgccga
      421 tacgcccgcc gcgctttcca cataaggcgt ggtggaagaa gtacccaaag ccgcacctgc
      481 cacaatggcg gtagagtctg caagcagtgc gcgtttcagg cggggcagct taccgtccac
      541 cagcagcccg gcacggtggg atatgccgac cagcgttccg gtactgtcaa atagatcgac
      601 caagaagaag acgaaaatca cactgaccat gctgacggta aacaggcctt caaaatccat
      661 ctgcataaaa gtcggcgcaa tgctcggtac ttcgccgatg atgccgtgaa attcattcaa
      721 acccatcagg ctggcaatga cggtaatggt caagatggtg atgatgattg cgccttgaac
      781 gcggaaatgt cccaatacga ccaccatagc aaaaccgaac agtgccaaca acgcggacgg
      841 ctgatgaata tcgcccaaac cgaccaaggt tgccggattg gcaacgataa tgcctgcgcc
      901 tttcagggaa atcagtgcca aaaacaaacc gataccggca gcaatcgaca ttttcaaacc
      961 cataggcagt gcgttgacca gcatttccct gactttaaaa aagctgaaca ggataaaaat
     1021 cagaccggag atgaacaccg cacccaacgc aacctgccaa ggcacgccca tacccttaac
     1081 gacggcaaag gtgaaatagg cattcagccc catccccggt gcgagtgcaa tcggatagtt
     1141 gccgacaaaa cccataacaa aacagccgat ggcagacgcg atacaggtag cgacgaatac
     1201 cgcccccata tccatgccgg tctcgcccaa aatcagaggg ttgacgataa cgatgtagca
     1261 catcgtcaaa aaagttgtca aacccgccat caactcggta cgcaccgtcg taccgtttgc
     1321 cttcagctta aaaatcccgt ccaacagtgt ttgttttgaa gtgtccatat ctgaaagtct
     1381 gtatcaagtg gaaaaatctg aatccgccga ccgcaaaccg tacgaaattg caacatcttg
     1441 aaaaacatac ctcccaaacc ggacaaaccg attcgggagg cagcaggttt acgggcaaac
     1501 agcacattat ttgtctgaat tgtgttgacg acgcaaaatc gcagggattt caaagtcgtc
     1561 aagtacggac tgattgtcga aatccgcagc ggtaaggttc atcgtgcgga taccgcgatt
     1621 ggtgcggatc ataccttcga cattgtggct ttgctcctgt ttggacgggg caaccgcttc
     1681 tacctccctt gccggaacaa aatcgaccgc gcctttttct ttcagaccgg tagcgataat
     1741 ggtaatccgg atggcatctt cgctcatggt ctcgtcttca gccgcaccga atttgcattc
     1801 caaatcggga tgcgcgcttt ggttgacgat tttcatgact tcggacaact cggacatttt
     1861 caagcaaccc ggagcagtcg taatattgac cagcacaccg cgcgctccgt ccaaggttac
     1921 atcgtccagc agcggactgg aaatggcctg gtcggtcgcc atacgcgcac ggtcgatacc
     1981 ttgggcataa cccgaaccca tcatagcgat accgcggttg ctcatcacgg ttttcacgtc
     2041 ggcaaagtcg aggttgatga tttcgctcgg gcaagttacc acttcggaaa tgcctgcgac
     2101 cgcatcgcgc aatacattgt cggcggcacg gaaggcttcg cgcatcgtta cgtcttcacc
     2161 caatgcagtc atcagtttgt cgttcgggat gatgatcagc gaatcgacgt gttctttcaa
     2221 ctgttctaac cctgcctgtg cgacatggac gcgcttacct tcatatgcga acggtcgggt
     2281 aaccacggca acggtcagaa tgcccaaaga cttggcaatc tcagcaacaa ccggcgcgga
     2341 accggtaccg gtaccgccgc ccataccggt cgtgataaac agcatattcg caccgcgaat
     2401 ggcttcttca atggcttccc ggtcttcctg ggctgccgca cggccgatat cgggattcgc
     2461 gcccgcgccc aaaccgcgtg tcagattcgt acccaactgg attctcttcg ccgcatggtt
     2521 ttttgccaga gactgcgcat ccgtattggc actgataaac tccacaccgc gcacattgtt
     2581 ggcaaccatg ttattgattg cattgcaacc gccgccgccc aagccgatta ctttaatcac
     2641 cgcagggctg actgccgatt ctgccacgtc gtaaacaaat tccattcaaa aactcctgct
     2701 cgccccattc agaggacggt ttaaataaat tattattcat tatataagaa gtatcttgct
     2761 gctggcaaaa tacttctcac ctgtcaaacg gcaatccacc tgttcagaag ctgttttcaa
     2821 tccaccgttt caatcttgcc aacaaaccgc cgcccccttc cctctcttgc actgcaccgt
     2881 tttccggctg cggcaagttt ccttccagct tgcatgctgc atgaagcagc ccgatggcgg
     2941 tagaaaaacg cggtgtgcgg acgcggtcgg acaaaccgcc catttcttgg ggtgcaccgg
     3001 tgcgtacagg caaatcgaag attttttcgg caaattccac aatcccggtc atcatggaca
     3061 caccgccggt cagaacgata cccgcattca gcacttcttt ggggaaaccc gatttttgca
     3121 gctcgcccag cactacgcca aaaatctcct gaatacgtgc actgatgatt gctgccaaaa
     3181 ccttactgga aacctgacgc gatgtccggt cacccacgcc cggaacttca atcatctcac
     3241 ccaagccttc cgtatcgcat gatgtcacgc cataatggat tttaatgtac tcggcggcat
     3301 cgagaggtgt tctcaacgat ttggacaaat ctttggtaat cagattacca ccggccggaa
     3361 tgacggacgt atggcggatg gcaccgttca tataaacggc aatatcggtc gttccgccac
     3421 caatgtcgat gacgcatacg ccgaggtctt tttcatcttc agtcagcacc gcctgcccgc
     3481 ttgccaacgg ctgaagcatg atttgatcgc ttttcaagcc gcaccgctcg atacattttt
     3541 ggacattctg cactgccgta cttgcaccgg taatgatgtg cacccgcgta tccagacgca
     3601 caccgctcat accgatgggc tccctcacgc caagttgggt gtcgataatg tagtcttgaa
     3661 ccacggcatc gagaattttt tgatcgggcg ggatattgat tgcctttgcc gtttcaatgg
     3721 cgcggtcgat gtctgcctgc gtgacttccc cgtctttaat tttaaccaca ccttgcgaat
     3781 tgagactgcg gatgtggttg cctgcgatac ctgtggtaac gtgggtaatt ttggtatccg
     3841 ccatcagctc ggcatcattg accgcctgcc tgatggcttg gacggtggca tcgatattgg
     3901 ttaccatgcc cgcgcgcaag ccccgtgaag gagcctgccc caaaccgacg atgttgattt
     3961 tgtcgtcatc ttgaacttcc ccgatcagtg cgaggacttt agacgtaccg atatccagta
     4021 cgctgatgta tctttgctgc tgttccattg ttcgtctgct cttaaaactg attgaaattt
     4081 gcgtcgcacc gtttcagacg gcacggtcgt aatctgtccg atacctgttc ccactattct
     4141 tcggattctt tttcgggtaa accgtcggaa gcatagcgga ctgaaaatcc gtccttatac
     4201 ctcatatcca cataggataa ccgattttta tttttacgca acagatgctg ccacgcttcg
     4261 gtaaaaagcc ggaggcgttt catctcgttt tcccgtccga gcctgacggt gatgccgttg
     4321 tccaaaacga caatccacgc cgaacgtgcc gtataggtca tctctttgat gcccaaaccc
     4381 tgttttgcca aaacagtcga aaattcgtca taacggcgga gcatttcggc agacgttcct
     4441 tccgcgcctc tgaataccgg cattccgggt ctgtccaagc gggcttcaaa aacattgcct
     4501 tcgccgtcca ccaaggcatg gtcgccccaa cgcgcgaccg gcttgcgctc ggtcaggacg
     4561 acctcaaccg tgtcgggaaa acggcggcgc accatgaccg acgcaatcca cggataccgg
     4621 cggtaggcct cctgtgcgcc attgatgtcc gtcctcaaaa tattcccatg gatgtattct
     4681 ttcgccaaac tgcccaatgc cttcttatcg gaataaacca ggttgccctt cagcgacacc
     4741 tgcttgacgg gcagatgatt cgaattgtaa aaccaaacca gcccggacgc agcaagcagc
     4801 atcgccatca tgacaagcag ccagcgcgtc agccgttcca tcgcttcggc attatcccac
     4861 atgtgcggtc ttcaaaattt caatacataa atcggcaaaa cccacgcccg taacggcagc
     4921 ggatttcggt actaaactat ggctcgtcat accgggcagg gtgttgattt ccaacagata
     4981 gagtttgccg tcggtatctt tgaggaaatc gacgcgcacg cagctttccg caccgattgc
     5041 ctgcgcgccg cgaaccgcca gttcgcgcat caggctttct tcggcttcgg tcagatcttc
     5101 cgaaggacat tgataaatgg tgtcgttgcg gttgtacttg gcttcgtagt cataaaactc
     5161 ggtcgcgggg atgatgtgta tgccgggcag gcctttgccg ttcaagacgg ggcaggaata
     5221 ttcgccgccg ccgataaacc gttcggcaat gatttcgccc tgaaggtgtt tcaattcttc
     5281 gtaaacgctt ttcagacggc cttttccttt gacttttacc acgcctacgc tgctgccttc
     5341 ggccgccggt ttcacaaaca tcggcaggcc caatttttct tcgacggcat cgaaatcagt
     5401 gtcgtcgtgc aggacggcga actcgggaac gggcagtccc aatgcctgcc aaatcagttt
     5461 gcagcggtat ttgtccatgc cgatggcgga tgcggcgaca ccgctgccgg tatagggaat
     5521 gcccaacagt tccaatgcac cctgaaccgc cccgtcttcg ccgtaagtac cgtgaaggat
     5581 gttgaatgcc gtctgaaaac cttgtgcctt caattcagac aatggggttt ctttaggatc
     5641 gaaggcatat gcgtctatgc ctttgctttt taaagcattc aaaatggcgg tgccgctgtc
     5701 cagcgagatt tctcgttcgc tggaaaaacc gcccatcaat acggccactt tgccaaaatt
     5761 ctgcattgtt tttgttcttt cctgattgct ttatgcttgt tgccagaggt cgtctgaaac
     5821 ctgatttgcg gtttcagacg acctttatat gatgttccgt ctgtcaggcg ggtgtgcctc
     5881 aaatctgttt cgacaattcc agcagcgcgg cggggacgcg gttgatgctt cccgcaccca
     5941 tattcaacac gatgtcgccg tcctgcaaaa cgttcaacag catttcgggc agatcggcaa
     6001 cgttttcgca gtaaatcggc tcgagtttgc ccaacacgcg gatggcgcgg gcaagagcgc
     6061 gggaatcggc ggcggcaatc ggctcttcac cggcggcata aacttcggtc agcaccagcg
     6121 cgtcaacggt attgaggact ttggtaaagt cttcaaacaa atcgcgcgtg cgggtatagc
     6181 ggtgcggctg gaaggcgagt accaaacgtt tttccggata cgcgccgcgt gcggcggcaa
     6241 gggtcgccgc catttcgacg gggtggtgtc cgtagtcgtc caccaagagc gcggtcccgc
     6301 cgtttggcaa cttgatgtcg ccgtattttt ggaagcggcg gccgacacct tcaaagccga
     6361 gcaagccttt ttggatcgct tcaaccgatg cgccgacttc cagcgccacg ccgatggctg
     6421 ccaatgcgtt cagcacgttg tgtctgccgg gcatattcag cacgacttca aacgacccct
     6481 gctcatgtcc tttcatttga acatggacgg tgaatttcat ttgcgcgccg acgttttcga
     6541 tgtcggtggc gtagatgtcg gcggtatcgt ccaaaccgta agtagcataa ggtttgctca
     6601 ctttgggcaa aatcgcgcgg acgtgttcgc tgtcaataca caaaaaggct ttgccgtaga
     6661 agggcatacg gtggatgaaa tcgataaacg cctgatgcag tttttcgacg ctgtgcccgt
     6721 aggtatccat atggtcttcg tcgatattgg taacgacgga cataatcggt gtcaggtgca
     6781 gaaaggatgc atccgactcg tcggcttcgg caacgatgta ttcgcctttg cccaagcggg
     6841 cgttggtgcc tgcggcgttg agtttgccgc cgataacgaa agtcgggtca agtcctgccg
     6901 cgccgaggat ggaggcggtc aggctggtgg tcgtggtttt gccgtgcgtg ccggcaatgg
     6961 cgatgccgtc acggaagcgc atcaactccg ccaacatcag ggcgcgcgga ataacgggaa
     7021 tttgctgctc caacgcagcg acaacttcgg gattttcttt tttgacggcg gtagaggtaa
     7081 cgacgacatc cgcaccgtta acgtgttcgg cggtatggcc gggataaact tgaatgccca
     7141 ggctgcccaa atgctcggta gcggcattac gcgcctgatc cgaaccggaa actttaaaac
     7201 ccaaattgtg caagacttcg gcgatgccgc tcatgccgac gccgccgata ccgacaaaat
     7261 ggatgttggt aactcgattt ttcatcataa tgttgcgttc cggtggattt tcgatgcgta
     7321 aaggcgttat tttaaagggc tgaccgtttg cgcgccatag ttttctgaca aatatatagc
     7381 ggattgaaat aaaaaacatc catgctgtct ggacggcatt tcagacggca tggttcggca
     7441 gtttacgccg cacacgcaat cgcggcttcg gcaacgtcgt ccgcgctgtg cggcaatgcc
     7501 aaagtacggg cgttttccgc ccatttgaga catttttcgc ggttcaggct gccgaggatt
     7561 tcggcgagtt tttccgccgt caactgggtt tgcggcaaca gcaatcccgc ctccgcctgc
     7621 accataaaac gcgcgttggc ggtttgatgg tcatcaacgg cgtgaggata cggcactaac
     7681 aacgcaccca atcccgccgc cgtcaactcg gcaatcgtca gcgcgccggc acggcaaatc
     7741 accaaatcgg catcgcggta ggcggacacc atatcggtaa taaattccac gcattccgct
     7801 tgcacgccca gcgcgtcgta atccgcctgc aagctgccca gcttgccccg tcccgattgg
     7861 tggtacatct gcggacgcgc attgtcgggc agcaaagcca atgcctgcgg tacggttttg
     7921 ttcaaaacgt ccgcgcccaa actgccgccg accaccaaaa ttttcagacg gccttcacgc
     7981 ccttggaagc gttcggcagg cacgggcagg ttgctaatat cggcgcggac ggggttgccg
     8041 accaagccgc cttcgtggct gaacgctttc ggaaaagcgt acaacacccg cttcgcccag
     8101 cgcgacaggt ggcggttgga caaacctgcc acggcgtttt gctcgtgaat cacaatcggc
     8161 acgcctaata gcttcgccgc caaaccgccg gggaaggtaa cgaagccgcc gaagccgatg
     8221 acgcactcga cacggtgttt gcggataatc cgctgcgctt cgcggacggt ttgatacaaa
     8281 gtaaacggca gcatcagctt gcgtttgatg ccgttgccgc gcacgccttt aatcgccagc
     8341 gtttccaagc gtatgccgta ttgcggcacg atacgctctt ccatcgaatc cttgctgccc
     8401 agccaaatca catgatggcc gcgcgcgcgc aatgaatccg ccaccgccag cgcggggaaa
     8461 atatgtccgc ccgttccgcc cgccatcagc ataaaggttt taccgcccat gatttactcc
     8521 acccgataac cgcgcatttt ccggcggttt tcataatcta tacgcaacag cagcatcata
     8581 ctgatcagca tgaaaaagac tgacgaaccg ccataggaca tcaacggcag cgtcagacct
     8641 ttggtcggca aagcaccgat gttcacaccg atattgaaga aactttggat accgatccaa
     8701 atgccgatac ccgaagcgat ataggcgttg aaagtcaaac ccaaatcgcg cgactgcttg
     8761 ccgatggaaa acgcccgcac caccagccag ccgtaacaga atatcagcac acacataccg
     8821 aagaaaccga attcttcggc gatgatggca aaaataaaat cggtatgcgc ttccggcaga
     8881 aagccgcgtt tgctcaaact cgcacccaaa cccataccga accactctcc gcgcccgatt
     8941 gccatcagag agtgggtaag ctggtagccg gcaccctgag ggtctttcca cgggtccaaa
     9001 aatgccacta cccgctgcac acggtaggga gcggcggtaa tcatcagcac catcccgccc
     9061 aagacgctgc ctaccaggac gaaaaaatat ttccacggca atcctgccag aaacagcatt
     9121 ccaacggcaa tgacggtaat gacgacaaac gaaccgaaat ccggctgtac cattatcagc
     9181 accaaaccga acgccaccag cataatcggc aggatgatcg cccggaaacg gccgtacatt
     9241 tctaatgttt cacgacgtgc ctgcggattg gtggcggaca tgatcagatt ggccgtcccc
     9301 cgccaaatcg actgccaacc caaactttcc atgctgcgca acacttcttc acggcgcgtg
     9361 aacaggcttg ccaaataaag gatgaccgcc agcttgaaca gctcggtcgg ctggaaattc
     9421 aacggaccca aaggtatcca acgggtcgcg ccattgattt cgcgcccggc aatcaatacg
     9481 actaccagca acaggccgga tagggcaaaa atccacggca caagccgccg ccatgtcctc
     9541 atcctgcaaa gaaaccataa aaaaccgctc gctatcaagc cggcaacgac gaaccccgcc
     9601 tgtctggtca aatagaaaaa ctgatcgccg ccttcttttg atgccaaata cacagaagcc
     9661 gaataaatca tcagcaggct gaacgccgtc atcagcacta ccatccacaa aagcggcgcg
     9721 tcgaatttcc tgccgtcgcg cacaatcggc ctgtcgagca gcagagtgtg gacaccgtcg
     9781 cccactttta ccaatacttc cgaaatcttc aaaaaaacca cctgccagtc tgtttgcacc
     9841 tgccgcaaag ggcaaaaatt tcagacggca gacaatgccg tccgaacata cgatacatcc
     9901 caaatcggta ttctaaatct ttacttgccg cccaacaatg acggcgtttg cgtttcagac
     9961 ggtatcacaa agccttaaac gccccgataa acacttccga acggtgcgca tagcctttaa
    10021 acatatcgaa gcttgcgcag gcggggctga gcaacacgat gtcgcccgct tcggcttggg
    10081 cgtatgccgt ctgaaccgct tcttccaaag tggcgcagtc ggtcatattc agatcgcagc
    10141 cgtccaaatc gcggcggatt tgcggcgcat cgacaccgat caggaacacg ccttttgcct
    10201 tgccgacaag cgcgtcgcgc aggggcgtga agtcctgccc tttgcccatg ccgcctaaaa
    10261 tcacgaagag cggattttgc aaaccggcaa tcgcggcggc agtcgcgccg acgttcgtgc
    10321 ctttgctgtc gtcgatgaac accacgccgt ttttctcgcc gattttttcc acgcggtgcg
    10381 gcaggccttg aaaggttttg acgtgttcga gcaatgcttc gcgcggcaaa ccgacggctt
    10441 cgcacaaggc aacggcagcc atcacgttgg tagcgttgtg caagccttgc aacggaatat
    10501 cttgcgtggc aatcaaatct tcattgcctt gtttcaggcg gcctgtctcg cgttccaacc
    10561 aaaaatcggc ttcgtgttcc aacgaaaacc atttcacctc gcgcccggcg cgcttcatgg
    10621 cgcggcagaa cacgtcgtcc gcattcaaaa cctgcacgcc atcgccacgg aaaatcttgg
    10681 ctttggtatg cgcgtagtcg agcaagtcgt cgtagcggtc gagatggtct tcggaaatgt
    10741 tcagcaccgt cgccgcagtc gggcgcaggc tttcggtgtt ttccagttgg aagctggaaa
    10801 gctccaacac ccacacgtcc gcctttttgc cttcgcgctg ccattccgcc tccaaaaccg
    10861 gcgtgccgat attacccgcg ataacggtat ccagcccgca cttgatgcag agatagccga
    10921 ccaggcttgt taccgtggtt ttgccgttgc tgccggtaat cgcaatcacc ttgtcgcctc
    10981 ggcggttcac aatgtccgcc agcaattcga tgtcgcccaa cacgcgcccg ccgttttgct
    11041 tgaacgcctc gatatccggc tgccgctcgc tgatgccggg gctgagtgcc agaatatcga
    11101 aaccgttgtc cagcgcatct ttcagacggc ctgtataaaa caccagcccg tcaaacatct
    11161 taccgatttg cgacacgcgc tccggtttca gctccgcatc atacgcggca acctccgcgc
    11221 cgtttttgcg caggtaggca atcatggaaa tgcccgtacc gccgagtccg gcgacgagga
    11281 ttttttggtt ttggaaagtc attttggttt tgccctaaaa caaatcatat tgagcagggg
    11341 ggatgtccgc tcctgctcaa gccactttca gacagcatcg cgagccgatt aataacccgc
    11401 cttcaggcgt tggtcattgt cgcagccgtc ttggtctccg ttttgacaag ccttgccaaa
    11461 ccattcttgt gcaagggcgc ggtcttggcg cacgccgcgt ctttcggcat acatcacgcc
    11521 caaattgttt tgggcttggg ctaccccctg cgctgccgcc tgccgaaacc atctgaccgc
    11581 ttcgacatcg tcttggcgca ctccacgtcc ttcggcatat atcacgccca aattgtattg
    11641 ggcttggaca accccctgcg ctgccgcctg ccgataccat ctgaccgctt cggtatcatc
    11701 ttggcgcacg ccgcgcccgt tggcatacat ccagcccaaa ttgtattggg cttgggctaa
    11761 cccctgttcc gccgcctgcc gataccatct gaccgcttca gcatcatccc ggcgcacgcc
    11821 gcgtcctttg taatacattg cgcccaaatt gtattgggct gctgcatttc cctgtgctgc
    11881 cgcctgcaag ttttcccgaa aatccgatac gtcatccgcc cacaccgctc ggttcaagcc
    11941 caaggcaatc agggcggcgg caagccattt gactgtctgt ttcatggttt tacttctgtt
    12001 ttagtataag gcgggtttca gccaccgtta acgatagggc tgggcggatt gtcgccgcag
    12061 gtttattgcg cgttcaaatg ccgtctgaaa gatgttcaga cggcataggt tcagcggatt
    12121 ttgagggtac tcaaaccgat caacaccaag acgatggtaa taatccaaaa gcggacgacg
    12181 acttgggttt ctttccagcc tttttgttcg tagtggtgat ggatgggcgc catcaggaag
    12241 atgcgttttt tggttttctt ataccagcca acctgaagca taacggatac ggcttctacg
    12301 acaaataatc cgcccataat gacgaggaca aactcttggc ggacgatgac ggcaacggta
    12361 ccgagcgcgg cacccaatgc caatgcaccg acatcgccca taaagacttg cgcgggatag
    12421 gcgttaaacc acaagaaacc gaggcacgcg ccgcacatgg cggtacagaa aatcaccact
    12481 tcgtttgcgc cggcaacgta aggtaattgc aggtattggg caaattgtga gtggccgctg
    12541 gcataggcga agatggcgag gccggcagca acgaggacga cggggaaggt cgcaaggccg
    12601 tccaagccgt cggtgaggtt gacggcgttg gatgtgccga cgatggtcag gtaggacaac
    12661 accaaaaagc cgaccacgcc cagcggcagg gcgatttgtt tgaagaacgg gacaatcaaa
    12721 atattgttgg cggaattggc ggcaaggtaa aacaatgcca aaccggcgat aatggcaacg
    12781 cttgactgcc acaccatttt gaatttggcg gacacgccgt tggggtcttt atagacgact
    12841 ttgcgccagt cgtcgtaaaa accgagcgca ccggtggcaa gcagtacgcc caagagaatc
    12901 cagatatacg ggttcgccca gttgccccac aacagagtgg acacggtaat ggcggtcaga
    12961 atcagcgaac cgcccatcgt cggcgtgccg tttttgacga ggtgggtttg cgggccgtcg
    13021 gtacgcactg cctgcccgca tttgagcgcg gtcagcctgc gtatcgtcca cgggccgaac
    13081 atcagggaaa acgccaaggc ggtcaacgcc gccatgacgg cgcggaatgt ggtgtattga
    13141 aaaatattca gaccggttaa ccagttgctg aaatgtgcga gccataaaaa catggggctt
    13201 cctttttttg ttttgt
//
LOCUS       DAWKST010000050        13145 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.050, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000050 DAWKST010000000
VERSION     DAWKST010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13145
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.050"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(47..1330)
                     /gene="hemL"
                     /locus_tag="WJL87_000959"
     CDS             complement(47..1330)
                     /gene="hemL"
                     /locus_tag="WJL87_000959"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ7799401.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPSEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTARTEQLVQG
                     FQTAADAAGIEFAADSVGGMFGLYFASHTPRNYADMARSNIDAFKRFFHGMLDRGIAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(1394..1807)
                     /locus_tag="WJL87_000960"
     CDS             complement(1394..1807)
                     /locus_tag="WJL87_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ7799402.1"
                     /translation="MKPAVWAALLLCACTSNFGDREHQFLRDQTEEKIVQFAVRGKTT
                     RAEVEARFGRQNPFGCYAYHEVSLPIYNFLPTNFIYMKSERRHWEWCVDYDGEGVVRD
                     YRFTHKVEKDERSVIRDAVGVIRKEAGKSLSQPEK"
     gene            2010..3338
                     /gene="miaB"
                     /locus_tag="WJL87_000961"
     CDS             2010..3338
                     /gene="miaB"
                     /locus_tag="WJL87_000961"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ7799403.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
     gene            complement(3423..5336)
                     /gene="dxs"
                     /locus_tag="WJL87_000962"
     CDS             complement(3423..5336)
                     /gene="dxs"
                     /locus_tag="WJL87_000962"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ7799404.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYNTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLEDLR
                     GRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKESAAQMPSEKEPKPAAKPTYTQ
                     VFGKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGVPVSDGMETVEIGKG
                     IIRREGGKTAFIAFGSMVAPALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(5421..6338)
                     /gene="xerC"
                     /locus_tag="WJL87_000963"
     CDS             complement(5421..6338)
                     /gene="xerC"
                     /locus_tag="WJL87_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ7799405.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDAGGVPQDLSRRDFTAALRRLSQRGLNARTLARKLSSWRQYCVWLVERGLLHTDP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            6532..7596
                     /locus_tag="WJL87_000964"
     CDS             6532..7596
                     /locus_tag="WJL87_000964"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ7799406.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVGAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMASCYAKGELNQIVK"
     gene            7616..8578
                     /locus_tag="WJL87_000965"
     CDS             7616..8578
                     /locus_tag="WJL87_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding family protein"
                     /protein_id="HEZ7799407.1"
                     /translation="MPSEPPFGRHLIFASLTCLIDAVYKKRYHNQDVFILSILRMTRS
                     KPVNRTAFCCLSLTTALILTACSSGGGGVAADIGAGLADALTAPLDHKDKGLQSLMLD
                     QSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGQLITLESGE
                     FQVYKQSHSALTALQTEQVQDSEDSGKMVAKRQFRIGDIAGEHTSFDKLPKGGSATYR
                     GTAFGSDDAGGKLTYTIDFAAKQGHGKIEHLKSPELNVELATAYIKPDEKHHAVISGS
                     VLYNQDEKGSYSLGIFGGQAQEVAGSAEVETANGIHHIGLAAKQ"
     gene            8717..9394
                     /gene="tsaB"
                     /locus_tag="WJL87_000966"
     CDS             8717..9394
                     /gene="tsaB"
                     /locus_tag="WJL87_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ7799408.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGETCLFHQEVGSRQSALI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGVPSLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIALPEGYVFSDG
                     IGNAFALENRPPFSGKPDMPTAADFLNLAADGGFPAVAAAHAELLYVRDKIALTAKEQ
                     AERRARP"
     gene            9391..9831
                     /gene="rimI"
                     /locus_tag="WJL87_000967"
     CDS             9391..9831
                     /gene="rimI"
                     /locus_tag="WJL87_000967"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ7799409.1"
                     /translation="MNIRRAVYADCEELAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGRLAAFIVWQNLPDESELHLIATAPAYRRRGVASALLEYWFANLPEGTQRLLLE
                     VRAGNTAAQVLYAAHGFTDAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            9825..10565
                     /locus_tag="WJL87_000968"
     CDS             9825..10565
                     /locus_tag="WJL87_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ7799410.1"
                     /translation="MLSARYLHLHEALGLGPMWLKRGAVVLPPATLPESPTQIRPQKQ
                     TVLSIPQRPSEQHTGQARLKTMKVLETTAVHTRKPAPETETPLSGVSDGIAPVSAASG
                     ITKLAVVSLCPPTEDMVYGQLFHGKAGVLLDNILKAVGLDAAYVHKTSWVKTAAVGNP
                     MPSEQAIANALGQIARELDGCRAPAVLFLGQAFVQPERQTMIETLCAGRPFFIIDHPA
                     RLLRQPELKARAWQVLKQLKRALQAGGS"
     gene            10627..11268
                     /gene="pyrE"
                     /locus_tag="WJL87_000969"
     CDS             10627..11268
                     /gene="pyrE"
                     /locus_tag="WJL87_000969"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ7799411.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRQSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIQFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            11342..11776
                     /locus_tag="WJL87_000970"
     CDS             11342..11776
                     /locus_tag="WJL87_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799412.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVTVPPAAPARQEGFNWTIATLFALIVLIMQLSYLVIL"
     gene            11773..13083
                     /gene="argA"
                     /locus_tag="WJL87_000971"
     CDS             11773..13083
                     /gene="argA"
                     /locus_tag="WJL87_000971"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ7799413.1"
                     /translation="MIVPDLFVAHFREAVPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFYLDMLQTAASAAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAGGETRRLISSAVAALEGGVHRVQILNGASDGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFTEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
BASE COUNT         2963 a         4071 c         3453 g         2658 t
ORIGIN      
        1 caatctgcca aacagatcga acagagaaac tctgtccgtc aaaacatcat gcagccatcg
       61 ccttgaacac ttcaaccgca accgcaaccg tttcgtcaat cagctcgggc gtatgcgcgg
      121 cagaaacgaa acctgcttca taagcggacg ggccgaaggc aatgccgcgg tcgagcatac
      181 cgtggaagaa gcgtttgaaa gcgtcaatat tggaacgcgc catatcggca tagtttcgcg
      241 gcgtatggct ggcaaaatac aggccgaaca tcccgcccac gctgtcggcg gcgaactcga
      301 tgcccgccgc atccgctgcc gtctgaaaac cttgaaccaa ctgttcggta cgcgccgtca
      361 ggttttcata aaacccttcg cggcggatga tttccagcgt tttcaaaccg gcggcgacgg
      421 caatcgggtt gcctgataat gtacctgcct gatacacgcc gcccagtggg gaaatacatt
      481 ccataatgtc tttgcgcccg ccgaacgcgg caagcggcat accgccgccg atgactttgc
      541 ccatcgtggt caggtcgggc gtgatgccgt gcagcgattg tgcgccgccg agcgcgacgc
      601 ggaaaccggt catcacttcg tcgtaaatca acaccgcgcc gtgtttttcg gtcaattcgc
      661 gcaaggcttt gacaaaggct tcagacggcc taaccagatt catattgccg acaaaaggct
      721 ccaaaatcac acaggcaatg tcattgccgc tttgagcaaa ggcttcttcc agttgggcga
      781 tattgttgta ctcgaggact aaagtgtgtt tggtaaagtc ggcaggcacg ccggcggaag
      841 acgggttgcc gaacgtcagc agaccgctgc cggctttcac caacaggctg tcggaatggc
      901 cgtggtagca gccttcaaat ttgatgattt tgtcgcggcc ggtaaaaccg cgtgccagac
      961 ggatggcggt catagtggct tcggtaccgg agctgacaag gcgcagccgt tcgacggacg
     1021 gcatgatttt ggcgatttgt tcggcaatgg cgatttcgcc ttcggtgggc gcgccgaacg
     1081 acaaaccgcc caacgcggct tcgcgcacgg cttcgacgac ttcggggtgc gcgtgtccga
     1141 caatcgcagg cccccaagag ccgacatagt cggtataacg cgtgccgttt tcgtcccaaa
     1201 catacgcgcc ttcggctttt ttgatgaagc gcggcacgcc gccgacgctg ccgaatgcgc
     1261 ggacggggga attcacgccg ccggggatga tggctttggc gcggtcgaat aaaatttcgt
     1321 tacggttcat atttatcctc aaatgccgcc tgaacggcag gtttcgggct tagaagcaga
     1381 aagtcccatt ttatcatttt tcaggttgcg acaaggattt gcctgcttct ttgcggatga
     1441 cgccaaccgc atcccggatg acggaacgct cgtctttttc cactttatgt gtaaagcggt
     1501 agtcccggac aactccctcc ccgtcgtaat ccacacacca ctcccaatgc cggcgttctg
     1561 atttcatata aatgaaattg gtcggcaaaa aattataaat aggcaggctg acctcgtgat
     1621 aggcataaca gccgaacgga ttttgccgcc cgaaacgcgc ttccacttcc gcccgggtcg
     1681 tcttgcccct aaccgcaaac tgcacgattt tttcttccgt ctgatcacgc aggaactgat
     1741 gttccctgtc gccgaaattg ctggtacacg cacacagcag caatgccgcc cacacagccg
     1801 gtttcataca cacctcccat taaagccaaa cattatacag ccgtcccgac cgattaaatt
     1861 catattttaa aacaatattc tgtttacaaa acccacatcg tgctataatc cgcaccgatt
     1921 ttcagacggc atcgtcatgc cgtctgaaat tttttcattc caacaacaat cagccccgcg
     1981 attacggctg cctgagaaag acacaaacca tgaaaaaagt atttatccgc accttcggct
     2041 gccagatgaa cgaatacgac agcgacaaaa tgctcgccgt cctcgccgaa gaacacggcg
     2101 gcatcgaaca ggttacccaa gccgacgaag ccgacatcat cttgttcaac acctgctccg
     2161 tgcgcgaaaa agcacaggaa aaagtgttct ccgatttggg gcgcgtgcgt ccgctcaaag
     2221 aaaaaaaccc cggccttatc atcggcgttg ccggctgcgt cgcctcgcaa gaaggcgaaa
     2281 acatcatcaa acgcgcgcct tatgttgacg tggttttcgg cccgcaaacg ctgcaccgcc
     2341 tgcccaaaat gattgtggac aaagaaacca gcgggctgtc gcaagtcgat atttccttcc
     2401 ccgaaatcga aaaattcgac cacctgccgc ccgcccgcgt cgaaggcggc gcggcatttg
     2461 tatcgattat ggaaggctgt tccaaatact gctccttctg cgtcgtcccc tacacgcgcg
     2521 gcgaagaatt ctcccgcccg ctcaacgacg tattgacaga aatcgccaac cttgcccagc
     2581 aaggcgtgaa agaaatcaac ctcttgggac aaaacgtcaa cgcctatcgc ggcgaaatgg
     2641 acgacggcga aatctgcgac tttgccactt tgttgcgcat cgtccacgaa atccccggca
     2701 tcgaacgtat gcgctttacc accagccatc cgcgcgagtt taccgactcg attatcgagt
     2761 gctaccgcga cctgccgaag ctggtttccc acctgcacct gccgattcaa agcggttccg
     2821 accgcgtatt gagcgcgatg aaacgcggct acaccgcttt ggaatacaaa tccatcatcc
     2881 gcaaactgcg tgccatccgt cctgatttat gcctgagcag cgatttcatc gtcggcttcc
     2941 ccggcgagac cgaacgcgag ttcgagcaaa ccttgaaact ggtgaaagac atcgccttcg
     3001 acttgagctt cgtgtttatt tacagtccgc gccccggcac gcctgccgcc aacctgccgg
     3061 acgacacgcc gcacgaagaa aaagtgcgcc gcctcgaagc cttgaacgaa gtcatcgaag
     3121 ccgaaaccgc gcgcatcaac caaaccatga tcggcacggt tcaacgctgc ctggtcgaag
     3181 gcatctccaa aaaagacccc gaccaactgc aagcccgtac cgccaacaac cgcgtcgtca
     3241 acttcaccgg cacgcccgac atgattaacc aaatgatcga tttggaaatc accgaggcct
     3301 acaccttctc cctgcgcggc aaagttgtcg aagcctaaac cctcacgccg aaaaaatgcc
     3361 gtctgaagcg tttcagacgg cattttgcct tgtatcggca gacgatggcg cggccgggcg
     3421 gcttaatttg ccgcatcccg atccgacaac cacgcgcgca cacgccgttc caccgcttcg
     3481 gcactcaagc ccaaatcgtc taaaagtttt ttcggatcgc cgtgtccggt tacggtatcg
     3541 gcaacgccca aaagcaaaac gggtttgcag atgccgtgtt tcgccaatac ttccagcacc
     3601 gcgccgcctg cgccgccctg ttcggcgttt tcttcaaggg taacgatgcg gtcgtggctt
     3661 cgggcaaggc ggacaatcaa ctcttcgtct atcggtttga cgaagcgcat atcggcgacg
     3721 gtggcgttca gtttttcggc aaccgccaat gcgggggcga ccatactgcc gaaggcaatg
     3781 aatgcggttt tcccaccttc gcggcggata atgcccttgc cgatttccac ggtttccatg
     3841 ccgtctgaaa ccggcacgcc cgtacccgtg ccgcgcggat agcggacggc ggcgggcgcg
     3901 tctgcctgat agcaggtcga aagcagcagg cggcattcgt tttcatcgct cggcgcggcg
     3961 acaatcatgt tcggcacgca gcgcaaaaag ctcaaatcgt acagaccggc atgggtcggg
     4021 ccgtccgcgc cgacgatgcc cgcgcggtcg acggcaaaca aaacgggtag gttttgcagg
     4081 gcgatgtcgt gcaccagttg gtcgtaggcg cgttgtaaaa aggtggaata aatcgccacg
     4141 acgggcttca tcccttcgca agccaaaccg ccggcaaagg taacggcgtg ctgctcggca
     4201 ataccgacat cgaaatagcg gtcggggaat cgttgttcaa actcaaccaa gccgctgccc
     4261 tcgcgcatgg cgggggtaat cgcaaccagt cgggaatctg ccgccgcccg gtcgcacagc
     4321 catttgccga acacttgggt ataggtcggt ttggcggcgg gcttgggttc tttttcagac
     4381 ggcatttgcg ccgcgctttc tttaggcagg ttggcgacgg cgtggtattt gacgggatcg
     4441 ttttcggcga gtttgtagcc gttgcccttt ttggtgatga cgtgcagaag ctgcgggcct
     4501 ttgcgtccgc gcaggtcttc caatacatcg accagatttt cgacgttgtg tccgtccacg
     4561 gggccggtat agcggaagcc gaagttttca aacaaagaca gtgactgttt ggcgtgttcg
     4621 gcttcttcgg caagggtttt gattttgtgt tcgacttttt gggcaaactc catcgcgccg
     4681 ggtattttgt ctaatacctt gcccgtttgc gctttgacgg tactcaacag gccgtgcata
     4741 tcgcgcacga cgttgctggc aaggtatttc ggcagcgcgc cgacgttggg ggaaatcgac
     4801 atttcgttgt cgttgaggac gaccagcaaa tccacatcca tatcgcctgc gcaattcaag
     4861 gcttcaaacg cctgccccgc cgtcatcgcg ccgtcgccga tgatggcgac gctgcggcgg
     4921 tcgctgccca agagtttgtc tgccgccgcc atgcccaacg ccgcgccgat ggaggtggag
     4981 gaatgcccca cgccgaacgc gtcgtactcg gactcgcaac gtttcggaaa acccgccaaa
     5041 ccgccgtatt ggcgcatggt gtgcatctgg tttttgcgtc cggtaaggat tttgtgcgga
     5101 tagctttggt gtccgacatc ccacaccagc ttgtcttcgg gcgtgttgta cacatagtgc
     5161 agggcgatgg tcagctcgac cgcacctaag ttgctggcga aatgaccgcc ggtttgaccg
     5221 acggattcca gcagaaaggc gcgcaactcg ccggcaaggc gcggcagctg ttttttgtcc
     5281 agacggcgca aatcttgcgg gctgtcaatc aggtcgagta gggggcttgg gttcatggtg
     5341 tgtctttttt atgtgtcgtc cgggtgcaac ggccaattat atatcaagag tgtgcggctg
     5401 acggctgatt ttgccgtatg tcattcgtcc tgccgcttgg cgcgcgggtg ggcttcgtca
     5461 tacaggcggg cgatgtggtc gaaatcgagt ttggtgtaaa tctgcgtggt cgaaaggctg
     5521 ctgtgcccga gcagctcctg caccgccctg atgtcgcgcg aagcctgcaa caggtgtccg
     5581 gcgtagctgt ggcgcatcat atgcggcgaa acgtgcctgc cgtcgccgtt ttgcgccgcc
     5641 cattgcgcca aacgtttttg gatttggcgt tggctcaggc gcgtgccgtt cctgccggta
     5701 aacagggctt tgccgtccga tgccgtctga cgcagcggca gatagttttt cagggcttcc
     5761 acgcttttgc cggtcagcgg cacctgccgc tgcttgcgcc ctttgccggt aacgtgtacc
     5821 cacgcctcgt ccaaatagac atcatctgca ttcaagccgt gtatctcgct cacgcgcaaa
     5881 ccgctgccgt acatcagttc gaacagggcg tggtcgcgca ccgccagcgg gtcgccgccg
     5941 tccacgggca aatccagcat ccggttcagc cattcctgcg gcagtgcttt gggtacgcgc
     6001 tcgggctgct tcggcggttt gatgtcggcg gtcgggtcgg tatgcagcag accgcgttcc
     6061 accagccaaa cacaatactg ccgccaagac gacagcttgc gcgccagcgt ccgcgcattc
     6121 aaaccgcgct gcgacagccg ccgcaacgcc gccgtaaaat cgcgccgcga caagtcctgc
     6181 ggcacgccgc ctgcatcttc agacggcatt tgtgccaaca gcgcaaacag ttcttccaaa
     6241 tcgcgccggt atgccgccac cgtgtgctcc gacttgccct cgcgcacgat gttttccaaa
     6301 taagcgtcaa aatgcgccgc aaacccgtcc aaaaccataa ccgccccaca caaatatcaa
     6361 aaaaacagtg aaaacattat cataaacgac acaccaaaac cccgaaccgc ccaaaatccg
     6421 acccgtccgc ccgccgttat cgttaacata aaatcaatga gccgtttatg gttttttgcc
     6481 gcaaaaaaca ttataatccg ccttatttac ctattgccca aggagacaca aatggcactc
     6541 gtatccatgc gccaactgct tgatcatgct gccgaaaaca gctacggcct gcccgcgttc
     6601 aacgtcaaca acctcgaaca aatgcgcgcc attatggaag ccgccgacca agtcggcgcg
     6661 cccgtcatcg tacaggcgag tgcaggtgcg cgcaaatatg cgggcgcgcc gtttttgcgc
     6721 cacctgattc tggcggcagt cgaagaattt ccgcacatcc ccgtcgtgat gcaccaagac
     6781 cacggcgcat cgcccgacgt gtgccaacgc tccatccaac tgggcttttc ctccgtgatg
     6841 atggacggct cgctgatgga agacggcaaa accccttctt cttatgaata caacgtcaac
     6901 gccacccgta ccgtggttaa tttctcccac gcctgcggcg tatccgttga aggcgaaatc
     6961 ggcgtattgg gcaacctcga aaccggcgaa gcaggcgaag aagacggcgt aggtgcagtg
     7021 ggcaaacttt cccacgacca aatgctgacc agcgtcgaag atgccgtacg tttcgttaaa
     7081 gataccggcg ttgacgcatt ggctattgcc gtcggcacca gccacggcgc atacaaattc
     7141 acccgtccgc ccacaggcga cgtattgcgt atcgaccgca tcaaagaaat ccaccaagcc
     7201 ctgcccaata cacacatcgt gatgcacggc tccagctccg ttcctcaaga atggctgaaa
     7261 gtcatcaacg aatatggcgg caatatcggc gaaacctacg gcgtgccggt tgaagaaatc
     7321 gtcgaaggca tcaaacacgg cgtgcgcaaa gtcaacatcg ataccgactt gcgccttgct
     7381 tccaccggtg cggtacgccg ctaccttgcc gaaaacccgt ccgactttga cccgcgcaaa
     7441 tacctgagca aaaccattga ggccatgaag caaatctgcc tcgaccgtta tcttgcgttc
     7501 ggctgcgaag gtcaggcagg caaaatcaaa cctgtttcgt tggaaaaaat ggcaagctgt
     7561 tatgccaagg gcgaattgaa ccaaatcgtc aaataacagg ttgactgtaa acaaaatgcc
     7621 gtctgaaccg ccgttcggac ggcatttgat ttttgcttct ttgacctgcc tcattgatgc
     7681 ggtatacaaa aaaagatacc ataaccaaga tgtttttata ttatctattc tgcgtatgac
     7741 taggagtaaa cctgtgaatc gaactgcctt ctgctgcctt tctctgacca ctgccctgat
     7801 tctgaccgcc tgcagcagcg gagggggcgg tgttgccgcc gacatcggtg cggggcttgc
     7861 cgatgcacta accgcaccgc tcgaccataa agacaaaggt ttgcagtctt tgatgctgga
     7921 tcagtccgtc aggaaaaacg agaaactgaa gctggcggca caaggtgcgg aaaaaactta
     7981 tggaaacggc gacagcctca atacgggcaa attgaagaac gacaaggtca gccgcttcga
     8041 ctttatccgt caaatcgaag tggacgggca gctcattacc ttggagagcg gagagttcca
     8101 agtgtacaaa caaagccatt ccgccttaac cgcccttcag accgagcaag tacaagactc
     8161 ggaggattcc gggaagatgg ttgcgaaacg ccagttcaga atcggcgaca tagcgggcga
     8221 acatacatct tttgacaagc ttcccaaagg cggcagtgcg acatatcgcg ggacggcgtt
     8281 cggttcagac gatgctggcg gaaaactgac ctatactata gatttcgccg ccaagcaggg
     8341 acacggcaaa atcgaacatt tgaaatcgcc cgaactcaat gtcgagcttg ccaccgccta
     8401 tatcaagccg gatgaaaaac accatgccgt catcagcggt tccgtccttt acaaccaaga
     8461 cgagaaaggc agttactccc tcggtatctt tggcgggcaa gcccaggaag ttgccggcag
     8521 cgcggaagtg gaaaccgcaa acggcataca ccatatcggt cttgccgcca agcagtaacc
     8581 attgtgaaaa tgccgtccga acacaataat ttaccgttcg gacggcattt ttgcgccata
     8641 ccgccctagg acacgacggc acggcaggca agccgatttc actatataat gccgtctgaa
     8701 ccaacgagag aatgccatgc aagctgattt caaccgcccc gtcctcgccg tcgataccgg
     8761 aacttcctat ttgtcgctcg cactgcgtgc cgacggcgaa acctgcctgt tccatcagga
     8821 agtcggcagc cgccagtccg cactgattct gccggaaatc cgcaccctat tccgaaatgc
     8881 aggcattacc gccgccgatt tgggggcgat tgtttatgcg aaaggccctg gcgcgtttac
     8941 gggactgcgt atcggcattg gcgtggcgca aggtttggca acgccgtttg ataccccttt
     9001 aatcggcgta ccctcgctcg atgccgccgc ctcgctgccg ccgccgcaaa gctgtatact
     9061 cgccgctgcg gacgcacgta tgggcgaagt gttttatgca tggttcgaca cgctgaaccg
     9121 ccgccgtttg agcgattatc aggtcgggcg ggcggcagac attgccctcc ccgagggata
     9181 cgtcttttca gacggcatag gcaatgcgtt cgcgctggaa aaccgtccgc ccttctcagg
     9241 caaacccgat atgccgactg ccgccgattt tctcaatctg gcggcggatg gcggttttcc
     9301 cgccgtcgct gcggcacacg ccgagctgct ctacgtccgc gacaaaatcg ccctgactgc
     9361 caaagaacag gccgaacgga gagcgcgccc gtgaacatcc gccgtgccgt ttatgccgat
     9421 tgcgaggagc tggccgcact cgatgccgtc tgcaacccgt ccgcatggac gcaacgccaa
     9481 ttcgaatccg cactcgtttc gccgtccgaa caggttttcc ttgcggaaaa agacggcagg
     9541 ttggccgcct ttatcgtttg gcagaacctg cccgacgaat ccgaactgca cctgattgcc
     9601 accgctcctg cataccgccg ccggggtgtt gcgtccgccc tactcgaata ctggttcgcc
     9661 aacctgcccg aaggcacgca acgcctgctg ctcgaagtcc gcgcaggcaa caccgccgca
     9721 caggtgctgt acgcggcaca cggctttacc gatgcgggca ggcggaaaaa ctattaccgt
     9781 acagccgacg gtaaaaccga agatgccgtc ttaatggaga aaatatgtta agcgcgcgct
     9841 acctccacct gcacgaagcc ttgggcttgg gcccgatgtg gttgaagcgg ggagccgtcg
     9901 tcctgccgcc cgcaacattg ccggaaagcc cgacacaaat ccgcccgcaa aagcaaaccg
     9961 tcctcagcat tccgcagcgt ccgtccgaac agcataccgg tcaggcacgg ctcaaaacca
    10021 tgaaagtgtt ggaaacaacc gccgtacata cgcgcaaacc cgcgcctgaa accgaaacgc
    10081 ctctgtccgg cgtttcagac ggcatcgccc ccgtttccgc cgcttcgggc ataaccaaac
    10141 ttgccgtcgt cagcttgtgt ccgccgactg aggatatggt ttacgggcaa ctgttccacg
    10201 gcaaagcagg tgtcctgctt gacaacatac tcaaagccgt aggactggat gccgcttatg
    10261 tacacaagac cagctgggtg aaaaccgccg ccgtcggcaa cccgatgccg tctgaacagg
    10321 ccatcgcaaa tgcgctgggt caaatcgcca gggaactcga cggctgccgc gcccccgccg
    10381 tccttttcct cgggcaggct tttgtccagc ctgaacggca aacgatgatt gaaactttgt
    10441 gcgccggccg tcccttcttc atcatcgacc atcccgcccg gctgttgcgc caacccgaac
    10501 tcaaagcccg cgcttggcag gtgttgaaac agttgaaacg cgccttgcag gcaggcggca
    10561 gttgaagcag gccgcacggg gcggtagaat cacaactgca tcgcaatacc tgacagaaag
    10621 caaaaaatga ccgatttccg ccaagatttc cttaaattct ccctcgccca aaacgtctta
    10681 aaattcggcg aatttaccac caaagccgga cggcagtcgc cctatttctt taatgccggc
    10741 ctctttaacg acggcttgtc cacgctgcaa ctggcaaaat tttacgcaca atccatcatt
    10801 gaaagcggca tccaattcga tatgctgttc ggccccgcct acaaaggcat tattttggcg
    10861 gcggcaaccg cgatgatgct ggcggaaaaa ggcgtaaacg tcccgtttgc ctacaaccgc
    10921 aaagaagcca aagaccacgg cgaaggcggc gtgttggtcg gtgcgccgct taaagggcgc
    10981 gtgctgatta tcgacgacgt gatttccgcc ggaacatccg tacgcgaatc gatcaagctg
    11041 attgaagcgg agggtgcaac ccccgccggt gtcgccatcg cgctcgaccg catggaaaaa
    11101 ggcaccggcg aattgagcgc ggttcaggaa gtggaaaaac aatacggcct gcccgtcgcc
    11161 cccatcgcca gcctgaacga tttgtttatc ctgttgcaaa acaaccccga attcggacag
    11221 ttcctcgaac ccgtccgagc ctaccgtcgg cagtacggcg tagaataaaa acaaagcata
    11281 tgccgtctga actgccttac gcctcagacg gcatcaaacc tgacacacac gaggaaatac
    11341 catgcccgcc tgtttctgtc cgcactgcaa aacccgcctc tgggtcaaag aaacccagct
    11401 caatgtcgcc caaggcttcg tcgtctgcca aaaatgcgaa gggctgttta aagccaaaga
    11461 ccatctggca agcacgaaag aacccatatt caatgatttg cccgaagctg tttcggatgt
    11521 caaacttgtt caccgtatcg gcacgcgcgc catcggcaag aaacagattt cccgtgacga
    11581 aatcgccggc atcctcaacg gcggcacaac ccagcccgat attccgcccg caaccgccgc
    11641 cacccctgct gccgcaccgc aggttaccgt accacccgcc gcgcccgccc gtcaggaagg
    11701 gttcaactgg acgattgcaa ccctgtttgc ccttatcgtc ctcattatgc agctttccta
    11761 cctcgtcatc ctatgatcgt gcccgacctc tttgtcgccc acttccgcga agccgtcccc
    11821 tacatccgcc aaatgcgcgg caaaacgctg gtcgccggca tagacgaccg cctgctcgaa
    11881 ggtgatacct taaacaagct cgccgccgac atcgggctgt tgtcgcaact gggcatcagg
    11941 ctcgtcctca tccacggcgc gcgccacttc ctcgaccgcc acgccgccgc gcaaggccgc
    12001 acgccgcatt attgccgggg cttgcgcgtt accgacgaaa cctcgctcga acaggcgcag
    12061 cagtttgccg gcaccgtccg cagccgtttt gaagccgcat tgtgcggcag cgtttccggg
    12121 ttcgcgcgcg cgccttccgt cccgctcgta tcgggcaact tcctgaccgc ccgtccgata
    12181 ggtgtgattg acggaaccga tatggaatac gcgggcgtta tccgcaaaac cgacaccgcc
    12241 gccctccgtt tccaactcga cgcgggcaat atcgtctggc tgccgccgct cggacattcc
    12301 tacagcggca agaccttcta tctcgatatg cttcaaaccg ccgcctccgc cgccgtctcg
    12361 cttcaggccg aaaaactcgt ttacctgacc ctttcagacg gcatttcccg ccccgacggc
    12421 acgctcgccg aaaccctctc ggcacaggaa gcgcaatcgc tggcggaaca cgccggcggc
    12481 gaaacgcgac ggctgatttc gtccgccgtt gccgcgctcg aaggcggcgt gcatcgcgtc
    12541 caaatcctca acggtgcctc cgacggcagc ctgctgcaag aactcttcac tcgcaacggc
    12601 atcggcacgt ccattgccaa agaagccttc gtctccatcc ggcaggcgca cagcggcgac
    12661 atcccgcaca ttgccgccct catccgcccg ctggaagaac agggcatcct gctgcaccgc
    12721 agccgcgaat acctcgaaaa ccacatttcc gaattttcca tcctcgaaca cgacggcaac
    12781 ctgtacggtt gcgccgccct aaaaaccttt accgaagccg attgcggcga aatcgcctgc
    12841 cttgccgtct cgccgcaggc acaggacggc ggctacggcg aacgcctgct tgcccacatt
    12901 atcgataagg cgcgcggcat aggcataagc aggctgttcg cactgtccac aaataccggc
    12961 gaatggtttg ccgaacgcgg ctttcagacg gcatcggaag acgagttgcc cgaaacgcgg
    13021 cgcaaagact accgcagcaa cggacggaac tcgcatattc tggtgcgtcg cctgcaccgc
    13081 tgaccgcaac ggaaagccgc cgcagaaatg ccgtctgaac cccgtttcag acggcatttc
    13141 cccga
//
LOCUS       DAWKST010000051        12979 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.051, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000051 DAWKST010000000
VERSION     DAWKST010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12979
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.051"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..388
                     /locus_tag="WJL87_000972"
     CDS             <1..388
                     /locus_tag="WJL87_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799414.1"
                     /translation="VADDLSALLQPAEAPAVEENAAEITLETPDSNTSEADALPDFLK
                     DGEEETVDWSIYLSEENIPNNADTSFPSESVGSDAPSEAKYDLAEMYLEIGDRDAAAE
                     TVQKLLEEAEGDVLKRAQALAQELGI"
     gene            complement(561..977)
                     /gene="gloA"
                     /locus_tag="WJL87_000973"
     CDS             complement(561..977)
                     /gene="gloA"
                     /locus_tag="WJL87_000973"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ7799415.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(1002..2171)
                     /gene="lapB"
                     /locus_tag="WJL87_000974"
     CDS             complement(1002..2171)
                     /gene="lapB"
                     /locus_tag="WJL87_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ7799416.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVVDGRPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDMNP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(2182..2496)
                     /locus_tag="WJL87_000975"
     CDS             complement(2182..2496)
                     /locus_tag="WJL87_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ7799417.1"
                     /translation="MKLIYTVVKIIILLFFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIIFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESTK
                     QP"
     gene            2806..3804
                     /gene="ilvE"
                     /locus_tag="WJL87_000976"
     CDS             2806..3804
                     /gene="ilvE"
                     /locus_tag="WJL87_000976"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ7799418.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINDDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(4048..4833)
                     /gene="fabI"
                     /locus_tag="WJL87_000977"
     CDS             complement(4048..4833)
                     /gene="fabI"
                     /locus_tag="WJL87_000977"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ7799419.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGADLAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(4988..5809)
                     /gene="dapD"
                     /locus_tag="WJL87_000978"
     CDS             complement(4988..5809)
                     /gene="dapD"
                     /locus_tag="WJL87_000978"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ7799420.1"
                     /translation="MSLQNIIETAFENRADITPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPTGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(5819..6049)
                     /locus_tag="WJL87_000979"
     CDS             complement(5819..6049)
                     /locus_tag="WJL87_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799421.1"
                     /translation="MPSERRRRGIKVPPPFCIDSGAEKAIPAARPIPKRQCSATARVL
                     LTNMRLRGTIAHSFQMNILPENTGKRPTKHLT"
     gene            complement(6019..7662)
                     /gene="pgi"
                     /locus_tag="WJL87_000980"
     CDS             complement(6019..7662)
                     /gene="pgi"
                     /locus_tag="WJL87_000980"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ7799422.1"
                     /translation="MNAFTRAWHALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCNLADAADLEGKMRALRTGAKVNNSEGRAALHTALRLPDGA
                     GAVYADGRDVLPEIRRELNRALKFAHSLDDGSYQGTTGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETACHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEEGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGTIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            8172..9404
                     /gene="pilG"
                     /locus_tag="WJL87_000981"
     CDS             8172..9404
                     /gene="pilG"
                     /locus_tag="WJL87_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ7799423.1"
                     /translation="MAKNGGLSLFSKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            9478..10338
                     /locus_tag="WJL87_000982"
     CDS             9478..10338
                     /locus_tag="WJL87_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ7799424.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            10340..10972
                     /locus_tag="WJL87_000983"
     CDS             10340..10972
                     /locus_tag="WJL87_000983"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ7799425.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFIILIRRVLTISAPVEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHNG"
     gene            10965..11174
                     /locus_tag="WJL87_000984"
     CDS             10965..11174
                     /locus_tag="WJL87_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ7799426.1"
                     /translation="MAESRQTRLQVKCPTCQTAVVWEPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDMAYR"
     gene            complement(11259..12934)
                     /gene="pilB"
                     /locus_tag="WJL87_000985"
                     /pseudo
     CDS             complement(11259..12934)
                     /gene="pilB"
                     /locus_tag="WJL87_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
BASE COUNT         2967 a         3606 c         3407 g         2999 t
ORIGIN      
        1 cgttgccgat gatttgtccg cactgttgca acctgctgaa gcaccggccg ttgaggaaaa
       61 tgcagcggaa atcactttgg aaacgcctga ttccaacacc tctgaagcag acgctttgcc
      121 cgacttcctg aaagacggcg aggaggaaac ggtagattgg agcatctacc tctcggaaga
      181 aaatatccca aataatgcag ataccagttt cccttcggaa tctgtaggtt ctgacgcgcc
      241 ttccgaagcg aaatacgacc ttgccgaaat gtatctcgaa atcggcgacc gcgatgccgc
      301 tgccgagaca gtgcagaaat tgctggaaga agcggaaggc gacgtactca aacgtgccca
      361 agcattggcg caggaattgg gtatttgatt cccaactgcc ctttcgcaga tcaaggatgc
      421 cgtttcagac ggcatctttt tttgccttat cggtgtaacg gataaagttt gaaccggcac
      481 aggctcaaac agcaggccga cggcaacaaa atgccgtctg aaccccctaa aggcttcaga
      541 cggcattggc ggcggcggta tcaggcagtt tgataggcaa ccgaatcgtc gccgcttttc
      601 ttttgaatga actcgatttt gtatccgtcg gggtcttcga cgaaggctat cacggttgtg
      661 ccgtgtttca tcgggccggc ttcgcggacg acgtttccgc cctgccgctt cacacgttcg
      721 caggcttcgt aggcatcgtc cacttcaacc gcgatgtgtc cgtaggcgtt gcccaagtcg
      781 tatcgttccg tatcccagtt gtgcgtcagt tccaaaaccg tgctgtcggt ttcatcgccg
      841 taaccgacga aggcaagggt aaatctgcct tcgggataat cttttcggcg gagcagtttc
      901 atacccaaaa cgttttggta gaaatcgagg gatttttcga gattgcccac gcggagcata
      961 gtatggagta agcgcatttt ttgtgttcct ttcggtggtg gttaaacttc gattttattc
     1021 ggggtaaacg tctgccattt gttgcaggcg gggcagtgcc agaaaaagac ttgggatttg
     1081 aagtggcagt tgcggcaacg gtacatcacg ctgcgctgta gctgccgtcc gataaccgaa
     1141 cgcatcatgt cggcatcggc tttccaagcc ggattcatat cgctgagttt caaaccgagc
     1201 aggcggtaca cgccgttaag gtcgggcttg cggcggacaa gctcgacggc ggtttgcgcg
     1261 gcttctttct cgcacttaag cagcagggat ttctcgtaca cgacattgat caggtcaagt
     1321 tcgggaaacg tctgcatata tcctgtcaga cggttcaagc cttcttcagg ttttccctgc
     1381 gcggcatagg cttcgtaaag cttctcgccg accatgctca agtatgcatg gttttgctgc
     1441 tcgatggcgg cataggcttc gacggcggca gggaaattgc cttgtcggtg ttcgatgtcg
     1501 cccaaaatca tgttggcgcg ggtgcatttt ttgttggctt cgagtgcctt gccgacattg
     1561 aaacgcgcga catcgaaatt ggacttgaac agcgcggctt gggcaagttc gcaataaaac
     1621 tgggcgattt caaactgata ggtctgatcg tcatggctga gcagccgggc ggtttcaacc
     1681 gctttttccc aatccctgtc ctgttggtag atattgagca ggtgctgtct ggcttcacgc
     1741 gccattttac cgtcttgcag ccccaaaaaa atctgttcgg cacgatcgac caaccccgca
     1801 ctttggtagt tttgcgccaa ttcaaacagg acgcgcgcac gcttttcgcc gaccgtatcg
     1861 ggagaatcga gcattgtccg gtgtatgttg atggctttgt cgttttcgcc acgctggcgg
     1921 taaagtttgc cgagggtgag gttcaaatca tacgattgcg gccggccgtc gacgacttcc
     1981 gccaactccc ttgccgcgcg cccgctgttg cggtcgacca aagcgtccaa gcttttataa
     2041 aatcccgaag ggatgctttt tgcctgcttc aatacggttt tcatatccac gcgggcggca
     2101 aaccagccca tcgcgaagaa gacgggcaaa aggataatcg gcagcaggat aatccacaat
     2161 tcgttgtcca tatcggcttt cttaaggctg tttggtagat tcgggcgcat tttgcgccgg
     2221 tggtgcggtc agctccttcc ccgtcaaacg cgcatttttc tttacttcgg cacgcaacct
     2281 gccgttctcg ccacgtaacg acaacaaccg tccgaacaag gcaaacattc caaaaataat
     2341 accgactaca aatgcgccga acaatacgac aatcagcggc aaatcgaatt tttgccccgg
     2401 caggtaggag aaggtaacgg catccgtatt aatgacggca agcagcagga agaacagcag
     2461 gataatgatt ttgacgacgg tatagataag tttcatgttt gctccgtaaa taaaaacgcc
     2521 gcccggacag cttcgcactc tgaaccaaga ttgcgctagt ttaaacgatt tgcacggttt
     2581 cggataggat gcggcagcgt gttcggacga catacggagt atgcgattgc cgacaatttt
     2641 accaaataca ccgttccttt ccatttgaaa aataacggat tggacaccgc atcgacagaa
     2701 aaacccgccg cgcacttgtc aaaaccctgt ttgcaggcgt atctttacaa tcttcaaatt
     2761 caaaccgttc attgaaacat atcagaataa gaaaggcttt acatcatgag cagacccgta
     2821 cccgccgtat tcggcagcgt ttttcacagt caaatgcccg tcctcgccta ccgcgaaggc
     2881 aaatggcagc cgaccgaatg gcaatcttcc caagacctct ccctcgcacc gggcgcgcac
     2941 gccctgcact acggcagcga atgtttcgag ggactgaaag ccttccgtca ggcagacggt
     3001 aaaatcgtac tgttccgtcc gactgccaat atcgcgcgta tgcggcaaag tgcggacatt
     3061 ttgcacctgc cccgccccga aacccaagcc tatctcgatg ctttggtcga actggtcaaa
     3121 cgtgccgccg acgaaattcc cgatgcgcct gccgccctgt acctgcgccc gaccttaatc
     3181 ggtaccgatc ccgttatcgg caaggccggt tctccttccg aaaccgccct gctgtatatt
     3241 ttggcttccc ccgtcggcga ctatttcaaa gtcggctcgc ccgtcaaaat tttagtggaa
     3301 accgaacaca tccgctgcgc cccgcatatg ggccgcgtca aatgcggcgg caactacgct
     3361 tccgccatgc actgggtgct gaaggcgaaa gccgaatacg gcgcaaacca agtcctcttc
     3421 tgcccgaacg gcgacgtgca ggaaaccggc gcgtccaact ttatcctgat taacgacgat
     3481 gaaatcatta ccaaaccgct gaccgacgaa tttttgcacg gcgtaacccg cgattccgta
     3541 ctgacggttg ccaaagattt gggctatacc gtcagcgaac gcaatttcac ggttgacgaa
     3601 ctcaaagctg cggtggaaaa cggtgcggag gcgattctga ccggtacggc agccgtcatc
     3661 tcgcccgtta cttccttcgt catcggcggc aaagaaatcg aagtgaaaag ccaagaacgc
     3721 ggctatgcca tccgtaaggc gattaccgac atccagtatg gtttggcgga agacaaatac
     3781 ggctggctgg ttgaagtgtg ctgatgcttt aaataaaaaa tgccgtctga aacccgtttg
     3841 gcgtttcaga cggcattttc gcatccgaac cgtttccgct gcacctgcag caagtcggca
     3901 caaaggcaat cggttaaaac aagcgtccgc atttcccatc ccgcctgccg taagtcgggc
     3961 atttccctag aaatacgctt cagacggcaa aacgccgccc gaaaccgata tgcggcacgg
     4021 acggcgcacg gatttgaaaa cggcggatta tccctcggtg ctcaaggcat tgatgctgta
     4081 accgccgtca acgtaagtga tttcgccggt aatgccggac gacaggtcgg acagcaggaa
     4141 ggcggcggta ttgccgactt cttcgatggt aacgttgcgg cgcagcggat tgtgggcggc
     4201 gacgtgtccc aagagtttgc cgaaatcggc gatgccggag gcggcaagcg ttttgatcgg
     4261 gccggcggaa atgccgttgc agcggatgcc ttctttgccc aagcaggcgg cggtaaagcg
     4321 gatgcctgcc tcaaggctgg cttttgccat acccatcacg ttgtagttcg gaatcgcgcg
     4381 taccgcgccc aagtagctca gggcgacgat ggcggagttt ctgccgcgca tcatcggacg
     4441 ggcggctttt gccagcgcgg gcaggctgta tgcggaaatt tcgtgtgcgg tgttgaacgc
     4501 ttcgcggctg atgctatcga ggaagtcgcc gctcaaggct tctttcggcg caaaaccgat
     4561 ggaatgcacc aaaccgtcca agccgtccca atgtttgccc aaatcggcga acacttggtt
     4621 gatttcgtcg tcgctggcga catcgcagcg gaatacgagt tcggaatcca attccgccgc
     4681 cattttgcgg acgcgctctt ccagtttgtc tacaacgtag gtaaacgcca aatccgcgcc
     4741 ttgttcgcgg caggctttgg cgatgccgta agcgatggaa cgctcggaaa tcatgccggt
     4801 aatcagaatt tttttgcctt gcagaaagcc cattttgtat ccttcaaata gtgttcggtt
     4861 cgttctaaac cgttgattat agcaaattgt ccctgtttct gtgttttcac gttgcagcgt
     4921 gcaaacggca atgccgtccg aagcggattt cagacggcat cggacgtttc aaatacggtt
     4981 taaggcatca gatgccgcgc aacaattcgt tcacgctggt tttcgcacgg gtttgcgcgt
     5041 ccacgcgttt gacgatgacg gcgcagtaaa ggctgtggct gccgtctttg gaaggcatac
     5101 tgccggacac gacgaccgaa cctgtcggta cgcggccttg gtagatttcg ccggtggtgc
     5161 ggtcgaagat tttggtggat tgaccgatga acacgcccat cgaaatcacg ctgccttctt
     5221 cgacaatcac gccctcaacg atttcagaac gcgcaccgat gaagcagttg tcttcaatga
     5281 tggtgggggc ggcctgcagg ggttcgagta cgccgccgat accgacgccg ccactcaagt
     5341 gcacgttttt accgatttgc gcgcaagagc cgacagtcgc ccaagtatca accatcgcgc
     5401 cttcgtcgac gtatgcgccg atgttgacgt aagacggcat cagcacgaca tttttcgcca
     5461 caaagctgcc gcgtcgggca accgcacccg gaacggcgcg gaagcctgcg tttttgaact
     5521 cgtcttcaga ccagtcggca aacttggtcg gcactttgtc gaagtatttg ttcacgccgt
     5581 cgttgaggac ttcgttgtct tggatgcgga aggacagcaa cacggctttt ttcgcccatt
     5641 cgttgacttt ccactcaccc acgcccaagc gttcggcaac gcgcagtttg ccggaatcga
     5701 gttggcggat ggtttccaac acggcttctt tgacttcggg agtaacggtg gtcggggtaa
     5761 tgtccgcgcg gttttcaaag gcggtttcga tgatgttttg caaagacata atgtttcctt
     5821 atgtgagatg tttcgtggga cgtttgccgg tattttcggg cagtatgttc atttgaaatg
     5881 aatgcgctat tgtgccacgc aaacgcatat tcgtcagcaa tacgcgggcg gttgccgaac
     5941 attggcgttt cggaatcggg cgggcggcag gtattgcctt ttccgcaccc gaatcaatac
     6001 agaacggcgg cggcactttt atgccgcgcc gccgcctttc agacggcatt cgcggtaaaa
     6061 cgccatcagc ccttcggtcg aggcatcgtg tacggacgtt ccgccttcca gttcgccgat
     6121 gatggttttt gccaactgtt tgccgtattc cacaccccac tgatcgaagg ggttgacgtt
     6181 ccatatcgtg ccttggacga aggttttgtg ttcgtaagcc gccatcagca tacccagatt
     6241 gtagggcgtg aggcggtcga gcaggatgct gttactgggg cggttgccgg ggaactcttt
     6301 gtgcggcgcg aggcgttcgc gttccgcttc gggcaaatct gccaattcgg cgcgtgcttc
     6361 gtccaaggtt ttgcctttca tcagggcttc agcctgtgca aaggcgttgg caacggtaaa
     6421 acggctgcgt ccttcctcca cgccctgcgc cgtcatcggg acgataaagt cgcagggaat
     6481 caggcgcgtg ccttgatgaa gcagttggaa ataggcgtgc tggcagttga ccccttcacc
     6541 gccgaacacg atgccgcccg ttttgcacac ggcgggactg ccgtctgaag cgcggctttt
     6601 gcccaaactc tccatatcga gctggttcag ccacgccggc agcaggcgca ggttgtggct
     6661 gtacggaacg gcggtctgcc cgtccgcgtg ctggaaattg ttataccaca cggcaatcag
     6721 tgccatcaga acgggaatgt tgcggcgcgg cggcgtgtgg aaaaaatggc tgtccatcgc
     6781 gtgcgccccc gccaacaact cgcggaaacg cgccccgccg accgcaacca tcacgggcaa
     6841 accgacgggc gaccagacgg aatagcgtcc gcccacccag tcgtacatcg caaacacgcg
     6901 ttccgccgcg ataccaaaag ccgcagctgc cgcagtgtcg gcagacaccg cgcaaaaatg
     6961 gcacgccgtt tcagattccg agaaccccgc accgcgatac cacgccttga ctgcctgtgc
     7021 attgagcagg gtttctggtg ttttgaagga cttgctggca acgcaaaaca ctgtcgtttc
     7081 ggggttcaga cggcataaaa ccgcatccag gcaggcagga tcggcgttgg cggcaaaatg
     7141 gacggcgata tgccgtctga acggctcaag tgcctgcacg cacattgccg gcccgaggtc
     7201 ggatccgcct atgccgatgt ggacaaaatc cgtaatccgt tttccggttg tcccctgata
     7261 cgaaccgtcg tccaaactgt gtgcaaactt caacgcacga tttaactcgc ggcggatttc
     7321 gggcaacacg tccctgccgt ccgcataaac ggcacccgca ccgtcgggca ggcgcaaagc
     7381 cgtatgcagc gcggcacgcc cctcactgtt gttgaccttc gcacccgtcc gcaaagcacg
     7441 cattttccct tccaaatccg ccgcatcggc aagattgcag agcagttgca gcgtatcttc
     7501 gcccaaacgg tttttgctgt aatcgaacaa catcccgtcc aaacgctcgt gcatacgctc
     7561 aaaacggtcg ggttcgcagg caaagcggtc gcgcaaaagg acatgacgcg tatcctgata
     7621 atggcgttcg agcgcatgcc atgcacgggt aaaagcattc atctgttttc cttgattttt
     7681 cagaaccgga ttaaaatgta gcagaatgta gtttaacaaa cggcagcggc tttggcgaat
     7741 ctccggaaca ccgcccgccg cgtccaatag caccttattt ccatatggcg aaaatatagt
     7801 gaattaacaa aaatcaggac aaggcggcga gccgcagaca gtacaaatag tatggaaccg
     7861 attcacttgg tgcttcagca ccttagagaa tcgttctctc ttgagctaag gcgagccaac
     7921 gctgtaccgg tttttgttaa ttcactatac cctccgaccg gcatttgacc cgcaacaata
     7981 tcctgcaaga tttattgtgt acgcataaat gccggacagc cgcctaaata aggtataatc
     8041 gcatccgatt ctgtcccgtt tgccgcccgc aggcgggatg tcggcggttt tcatcttacc
     8101 gggaacacgc cggtactgtt ttttaacgat gtttcttaca ttttattcca attactttac
     8161 ggggctagaa tatggctaaa aacggaggac tttctttatt ttcaaagaaa gaaaaacgct
     8221 ttatctttga aggcaggcat tccgcctccg acaaactggt caacggcgaa gtatccgcgt
     8281 ttaccgaaga agaggcgcgc aaaaaactgg caaaacgcgg catccgcccg ttgcagatta
     8341 cccgtgtgaa aacaagctcc aagcgcaaaa tcacacaaga agacatcacc gttttcaccc
     8401 gccagctgtc cacgatgatt aaagcgggcc tgccgctgat gcaggcattt gaaatcgtgg
     8461 cgcgcggaca cggcaacccg tctatgacgg aaatgctgat ggaaatccga ggcgaagtgg
     8521 aacagggcag ctcgttgagc cgcgcattct caaatcaccc gaaatatttc gaccgcttct
     8581 actgcaacct ggttgcggcg ggcgaaacgg gcggcgtatt ggaaagcctg ctggacaaat
     8641 tggcaattta caaagaaaaa acccaggcca tccgcaaaaa ggtaaaaacc gcgctgacct
     8701 atccggtatc cgtcatcgcc gtcgccatcg gtttggtatt cgtgatgatg attttcgtac
     8761 tgcccgcctt taaagaagtt tacgccaata tgggcgcgga gcttcccgca ctgacccaaa
     8821 cagtgatgga tatgtccgac tttttcgtct catacggctg gatggtgctg atcgcactgg
     8881 gctttgccat atacggcttc cttaaattga aggcgcgttc gattaaaatc caacggcgta
     8941 tggatgccat actgctgcgt atgccgattt tcggagacat tgtccgcaaa gggacgattg
     9001 cccgctgggg caggacgacg gcgacgctga ttgcggcagg cgtgcctttg gtcgatgtat
     9061 tggactccac tgccggcgcg gcgggcaatt taatctatga agaagccacc cgggaaatcc
     9121 gtacgcgcgt catccaaggt ttgtctatga cttcggggat gcgtgcgacg gaactgttcc
     9181 ccaatatgat gttgcagatg tcttccatcg gcgaggaatc gggttctttg gacgatatgc
     9241 tcaacaaagc cgccgaattt tacgaagacg aggtggacaa tgcggtcggc aggctgtccg
     9301 ctatgatgga gccgatcatt atcgtgattt taggcttggt catcggcacg cttctggtcg
     9361 ccatgtatct gccgctgttt aatttgggca acgtggtcgc ctgatttgcc gcacagatcc
     9421 ggcgcggatt cgctctgcgc cggtttgttt ttgttttgaa tacattgaga acaaaatatg
     9481 tctgatttgt ctgtattgtc gccgtttgcc gtgcctttgg cagcggtgtt cgggctgctg
     9541 gtcggcagtt tcttaaatgt cgtcatttac cgcgtgccgg tcatgatgga acgcggctgg
     9601 acggtatttg ccaaagaata tttaaacctg ccgctgaccg aagaggaaag ccgtaccttc
     9661 aacctgatga aaccggattc ctgctgtccc aaatgccgcg tgccgatacg cgcgtggcag
     9721 aacatcccga ttgtcagcta cctgctcctg cgcggcaaat gcgcctcctg ccaaaccaaa
     9781 atcagcatac gctatccctt aatcgaactg ctgaccggcg tattgttcgg gctggttgcc
     9841 tggcaatacg gctggtcttg gattacattg ggcggtttga tactgaccgc gtttctgatt
     9901 tccctgacct ttatcgatgc ggacacccaa tacctgcccg actcgatgac attacccttg
     9961 atttggctgg ggctgatatt taatttggac ggcggcttcg tgcctttgca gtctgccgtt
    10021 ttaggtgcgg ttgccggcta tggttcgata tggcttttat gcgcggtata taaactgatt
    10081 aaaggcgaaa tcggtatggg caacggagat ttcaaactga ttgccgcatt gggcgcgtgg
    10141 ctcggcatat ccgcattgcc cgtgctgatt tttgtttcct cgctgatcgg tttggtcgcg
    10201 gcaatcgtta tgcgcgtcgc caaggggcag cattttgcct tcggccccgc actgacagtt
    10261 tcgggctgga taatttttac ggcaaacgat tccgtatggc gggcggtcaa ctggtggctg
    10321 acccatccgg tgctgtaaga tgacggtatg ggtcggactg accggcggaa tcggcagcgg
    10381 caaatcggca gccgcgcaat gttttgccga tttgggcgtg ccgcgcatcg atgcagacgc
    10441 ggcggcgcac tcgctgacgg cttcagacgg catcgccctg ccggaaatca ggcggctgtt
    10501 cggcgacacc gttttcgaca cacagggttt gttgcggcgc gacatattgc gtaaagaagt
    10561 ctttgcctcc ccatcgcgaa aagccttgct cgaatccgtg atgttgccgc tgattttctc
    10621 agaaatcaaa aaacagcaag aaacctttac cgatgcggct tacggcattg tcgaaattcc
    10681 gctgctgacg gaaaagcgtc aatttatcat cctgatacgg cgtgtcctga ccataagtgc
    10741 ccctgtggaa aaacgtatcg gcagggtgat ggcccgcagc gggctgacgc gcggcgaggt
    10801 ggctgccgtc atcagccatc aggcatccga atccgaacgc ctgctgcttg cagacgatgt
    10861 gctgctcaat gacggcagcc tcaaaagcct gcgtgagaaa acaatgcgcc tgcacgcgtt
    10921 ttattcaggg attttcgcct caaaaccaac acaaggaaaa cacaatggct gaatcgcggc
    10981 aaacacgcct tcaagtcaaa tgtccgacct gtcaaacagc agtagtatgg gaacccgaaa
    11041 acgcattccg ccccttctgt tcgcaacgct gcaaactgat cgacttgggc ggatgggcag
    11101 acgggaaata tacggtttcc ggccaaacgg aaagtttgcc ggaaatatcc gaacccgaca
    11161 tggcataccg ctgaccgccc cgccttcccg gcaaacaccc tgaaagtcaa atgccgtctg
    11221 aaacaaacac gcttcagacg gcattttcat tctcaaacct aatcgttggt atttgccgtt
    11281 acctcttcca atgaagtaat gccctgcata actttcaaaa taccggcccg gcgcaaatcc
    11341 accataccct ccttataggc aacgtccaaa atatccactt ccgtaccgtt gttcataatc
    11401 acacgctgca tttcttcgct gatgggcata acctcataca cgcccgcacg ccccttataa
    11461 ccctgccccg gcaacggtcg caaccgacgg cgcggtaaag tttccaatct tttgcaaggt
    11521 cctcatcggt gaagccgact tccttcaaag cagaggcaga cgggcgttcc acttcctgtt
    11581 tgcagctcga acacagcctg cgtaaaagac gctgcgccat aatcaggctg accgaactgg
    11641 caatattaaa cggcgcgaca cccatattca gcatacgcga caacgtcgcc ggcgcattat
    11701 tggtgtgcag ggtggaaaac accatatgcc ctgtttgtgc cgccttaatc gcaatatcgg
    11761 cagtttccaa atcacgaatc tcaccgacca taatgatgtc cgggtcctga cgcaggaaag
    11821 acttcaaagc agcggcaaaa gtcagaccct gcttatcatt gacgttaacc tgattgatgc
    11881 ccggcaggtt aatctcggca gggtcttccg ccgttgcaat atttaccgac tccgtattca
    11941 aaatattcaa acaggtatag agcgacaccg tcttacccga acccgtcgga ccggttacca
    12001 gcaccatccc gtagggacgg tgaatcgctt ccaacaacaa ttttttctga aacggctcaa
    12061 aaccgagctg gtcgatgttc aaagacgcgg catcggaatt caaaatccgc atcacgacct
    12121 tttcgccaaa cagcgtcggc aatgtgctga cacggaaatc gacaggcttg ccgccctttt
    12181 gaaaggtcag ctgcatcctg ccgtcctgcg gtatccgttt ttcggaaatg tccaaacgcg
    12241 acattacctt aatccgggaa gcaagctgcc cccttaccgc aatgggcggc tgaaccacct
    12301 cgcggagctg cccgtccaca cggaaacgga tacgggcatt gtgttcgtaa aactcgaaat
    12361 ggatgtccga tgccccgctg cgcaaggcat ccgacaaagt cttatggata aacctcggaa
    12421 cagggccgtc ttctgcctcc tcgttgtcga tatacagggt gtggctttcc tcttcctcct
    12481 gcccctcccc aagctcctga agcagcgatg tcgaacgcga acccacccaa tcgagcaaac
    12541 ccgccaactg gtcatcctcg acaatgacca actcaacctc aatccctgcg gcagaaacgg
    12601 ttttctgaat ttgcggcatc tgtgtcggat cggaaaccgc aaaaaatact ttgtcgcccc
    12661 gacggaaaac cggcaaacag tggaactcca ccatctgctc ctccgtcaac acccccatca
    12721 gcaccctgtg gcgcggataa tgacgcaaat caagaatcga ataactgaac accctcgcaa
    12781 tcaatgccgc aagcgacttg ggcgaaatga caccgtctga aaacagcatc ggcaacactt
    12841 ccttacccgc ctgcgactca ttgcagtaat gctcggcctg ctcaacagta accacctggt
    12901 tttgaaccag aatcctcagc aaacctacgc tcatacgacc ttatccccaa atttattcat
    12961 tgttatacct gtacagctt
//
LOCUS       DAWKST010000052        12719 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.052, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000052 DAWKST010000000
VERSION     DAWKST010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12719
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.052"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(215..1066)
                     /locus_tag="WJL87_000986"
     CDS             complement(215..1066)
                     /locus_tag="WJL87_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEZ7799427.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QESACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLKQALTALQKFDPAGVAA
                     ADLNESLILQIERSGECAAKPAALHIVRNALDSIDGNRSQTLARIKKHLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESLEIPAA
                     HKRKTAE"
     gene            1415..2929
                     /gene="katA"
                     /locus_tag="WJL87_000987"
     CDS             1415..2929
                     /gene="katA"
                     /locus_tag="WJL87_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ7799428.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGIGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
     gene            3253..3465
                     /locus_tag="WJL87_000988"
     CDS             3253..3465
                     /locus_tag="WJL87_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ7799429.1"
                     /translation="MGCKVGVGWASAHQLHQSHQPHQSANLTGTGKPMPSESGRIGGG
                     GGLKPTLQPTNPTNPLFRVIPTKVGI"
     gene            3583..3831
                     /locus_tag="WJL87_000989"
     CDS             3583..3831
                     /locus_tag="WJL87_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799430.1"
                     /translation="MHRTGDRVDVGWASAHQSHHSTNSANLTGAGKPMPSEGGGIGGG
                     GGLKPTLQPPPPKKNKKKPNLTKSDKKCQPETDKNCHN"
     gene            4088..4534
                     /locus_tag="WJL87_000990"
     CDS             4088..4534
                     /locus_tag="WJL87_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ7799431.1"
                     /translation="MKAIQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQMSEAL
                     TLAEGQKSAVVEYYSDNGTFPNSNTSAGIAASNEIKGKYVASVKVEGNASVASITATM
                     NSSNVNKDIKGKTLVLVGKQNSGSFSWECKKGSVDEKFLPSTCRTK"
     gene            4988..5488
                     /locus_tag="WJL87_000991"
     CDS             4988..5488
                     /locus_tag="WJL87_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7799432.1"
                     /translation="MSNYRRDFTRGAHYFFTLALQDRTKDYLIRHIDRLRLAYRETKT
                     RYPFETAAICILPDHLHFIMRLPEGDHDYPIRIAYLKTRFSQLIPKECRAPNGSKTKK
                     REAGIWQRRFWEHLIRDEQDLNNHRDYIYFNPVKHGYAASVKEWRYSSFFKDVEKGIY
                     PLDWGG"
     gene            5952..6518
                     /locus_tag="WJL87_000992"
     CDS             5952..6518
                     /locus_tag="WJL87_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEZ7799433.1"
                     /translation="MSLYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMMREGLSGKAEDVRESKRES
                     LWMTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAVIGMATTVMVTVGLTAGRALGVLF
                     GKRAEFAGGLVLIAIGTWTLLSHLGLIG"
     gene            complement(6952..8988)
                     /locus_tag="WJL87_000993"
     CDS             complement(6952..8988)
                     /locus_tag="WJL87_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ7799434.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVTDTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPKDSLAMFAAAAQSESKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDDGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLGLADLQPWDLG
                     YAGEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFADGTLQLPTAYLVCNFTPPVG
                     GKEARLSHDEILTLFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSTHEETGEPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKEVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPRIDALLRHSGFDNAA"
     gene            complement(9076..9948)
                     /locus_tag="WJL87_000994"
     CDS             complement(9076..9948)
                     /locus_tag="WJL87_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ7799435.1"
                     /translation="MEIWNRLDTWLGAVPIRAEAVESVAAVAALLLACALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            10218..12608
                     /gene="gyrB"
                     /locus_tag="WJL87_000995"
     CDS             10218..12608
                     /gene="gyrB"
                     /locus_tag="WJL87_000995"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEZ7799436.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLNV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDSRTIEGEELANTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
BASE COUNT         2998 a         3438 c         3509 g         2774 t
ORIGIN      
        1 cattcccgcc aaacccccgt cattcccgcg aaagcgggaa tccaggttcg ttgagtttcg
       61 gtcatttccg ataaattctt gcagctttgc gtttctagat tccctctttc gcgggaatga
      121 cgggatgcag gtttccgcgc ggacggattc ggcattcccg ctttcgcaga aatgccggct
      181 tttggtttgt tgtctttata agattattca gcaattattc tgcggttttg cgtttgtgtg
      241 ctgccggaat ctcaagggat tctctgtatt tggcaatggt gcggcgagaa acctccatgc
      301 cgcggaaagc cagcaggttg gcgagtgcct cgtcagaata cggcgtgggt gtggacgagg
      361 caaaaccggc ggcgggaaag ggattgagcg aagcaatgag gtcgagtgcg gcttcgagtg
      421 tgccgctgtc ggtttggggc aggtgttttt ttattcgtgc gagggtttgg ctgcggttgc
      481 cgtcaatgct gtcgagggcg tttcggacga tatgcagggc ggcgggtttg gcagcacatt
      541 cgcccgatct ttctatctgc agtatcagcg attcgttcaa atcggcggcg gccacgcctg
      601 ccgggtcgaa tttttgcaat gcagtcaggg cttgtttcag cattgcttca tccaacatcc
      661 actccaaggg cgtatggtcg aggatgtctt cgatgctgtc ggtcagataa ccttgctcgt
      721 caaggaaatc gatgaggatg tggacacagg cggattcttg gtcggaaagc gggtgttcgc
      781 atacttgcgc gtgcaggtat tgcttgaaat cctgctcgcc ggcgatgttg gacagcatat
      841 cttcgccttc gtctccgccg atttgacggg caggcgcagt gtaatggctg aactcggcat
      901 cggaaaattc atccgtgtct ttgcgttcga gcagggggtt gtccgacagc cagttttcga
      961 cctcgcgttc aagttcgata cccgacatct gcaatacgcg caaagattgt tgcagccgct
     1021 ggttgagctg ctgggtctgt ttgagcttta ttccgagtaa ggtcatgata atgtgggaga
     1081 aattgttatt ttcagcctgt cggcgcaaaa aaatgccgca aagcgtcatt gcattataaa
     1141 tggttttaat gagcgggttc ggattccgtt cgataacaaa aaacaaacga aaatcaaaaa
     1201 ccgattgctt ataacaatat taaatcgatt tcatagtttt aatagcgaga atcttggcgt
     1261 atagtcgcat ccatagtttt tacaaaagga aaacaaaatg tcgattcaag aaatttattg
     1321 caatcaagaa accggttacg aatacgcttt ccgccaaatc gtactgtaag ccgtttaccc
     1381 ccatttgacc aacctgagaa aaggaacaag agcgatgact acctccaaat gccccgtaac
     1441 ccatctgacc atgaacaacg gcgcgcctgt tgccgacaat caaaacagcc tgaccgccgg
     1501 tcctcgcggc cctctgctgg cgcaggattt gtggctgaat gaaaaactcg ccgacttcgt
     1561 gcgcgaagtc atccccgaac gccgtatgca cgccaaaggt tcgggcgcgt tcggtacgtt
     1621 taccgtaacg cacgacatca ccaaatacac ccgcgccaaa atcttcagcg aagtcggcaa
     1681 aaaaaccgag atgttcgccc gtttcaccac cgtggcaggc gaacgcggcg cagccgatgc
     1741 agaacgcgac atccgtggct ttgcgttgaa attctatacc gaagaaggca actgggatgt
     1801 ggtcggcaac aacacgcccg tgttcttcct gcgcgatccg cgtaagttcc ccgacttgaa
     1861 taaagccgtc aaacgcgatc cgcgcaccaa tatgcgttcc gccacaaaca actgggactt
     1921 ctggacgctg ctgcctgaag cgttgcacca agttaccatc gtgatgagcg accgcggcat
     1981 ccccgccggc taccgccata tgcacggctt cggttcgcat acctacagct tctggaacga
     2041 agcaggcgag cgtttttggg tgaaattcca tttccgcacc caacaaggca ttaaaaacct
     2101 gaccaacgaa gaagccgcca aaatcatcgc cgacgaccgc gaaagccatc agcgcgactt
     2161 atacgaagcc atcgaacgcg gcgagtttcc gaaatggacg atgtacatcc aagtcatgcc
     2221 tgaagcagac gcggaaaaag taccttatca tccgtttgac ttgaccaaag tttggccgaa
     2281 aaaagactat ccgctgattg aagtgggcga attcgagttg aaccgcaatc ccgaaaactt
     2341 cttcgccgat gtagaacaat ccgcctttgc tccgagcaac cttgtgcccg gtatcggcgc
     2401 gagccccgac aaaatgctgc aagcgcgttt gttcaattac gccgacgcgc aacgctaccg
     2461 cttgggcgtg aacttccgcc aaatccccgt caatcgtccg cgttgccctg ttcacagcaa
     2521 ccagcgcgac ggacaaggcc gagccgacgg caactacggc agcctgccgc actacgaacc
     2581 caacagcttc ggacaatggc agcagcagcc cgacttcgcc gaaccgcctt tgaaaatcaa
     2641 cggcgacgcg gcacactggg actaccgcca agatgatgac gactacttca gccaaccgcg
     2701 cgccctgttc aacctgatga acgacgcgca gaaacaggca ttgttcggca acaccgccgc
     2761 cgcaatgggc gacgcgcccg acttcatcaa ataccgccat atccgcaact gctaccgttg
     2821 cgatccggca tacggtgaag gcgtggcaaa agccctcgga ctgactgtcg aagacgcaca
     2881 agccgcccgc gcgaccgacc ccgcactcgg ccagcccggt ttgctgtaag ggggcattat
     2941 gtggatggaa attgaagaaa tcctgtcccg cgccgtccgc taccggaaat aaccgggcat
     3001 aaaaatgccg tctgaaacat tgtccgaccg tttcagacgg cattccccca tcccgccccg
     3061 ccgtttcagc gggcgttttt tattaaacgc aaaatatccc gtcattccca cgcaggcggg
     3121 aatctagaac gtaaaatcta aagaaacctt tttccccata agtttccgca ccgacagacc
     3181 tggattcccg cctgcgcggg aatgacggtt taggtatttc tgacgattca gtaagccgta
     3241 tcggactgta gaatgggctg taaggtgggc gtagggtggg cttcagccca ccaactccac
     3301 caatcccacc aaccccacca atccgccaat cttaccggta ccggcaaacc gatgccgtct
     3361 gaaagcggca gaatcggcgg aggcggtggg ctgaagccca ccctacagcc caccaatccc
     3421 accaacccac ttttccgcgt cattcccacg aaagtgggaa tctagaacgt aaaatctaaa
     3481 gaaacctttt tccccgataa gtttccgtgc cgacaggtct ggattcccac ctgcgcggga
     3541 atgacggttt aggtattcac ataaggcgga tgtgtaaggt agatgcatag gacgggcgat
     3601 agggtggacg tagggtgggc ttcagcccac caatcccacc attccaccaa ctccgccaat
     3661 cttaccggtg ccggcaaacc gatgccgtct gaaggcggcg gaatcggcgg aggcggtggg
     3721 ctgaagccca ccctacagcc acccccccca aaaaaaaaca aaaaaaaacc caatctgaca
     3781 aaatctgaca aaaaatgtca gccggaaact gacaaaaatt gtcacaactg acaaaaaacg
     3841 tcagcccgcc gcattattcc gccgcaggcc gcgccgcgtt gatataaaaa aataccaata
     3901 ccaaatagac aaataaaaaa tattctgtaa aatgtcgagc cgaacccctt tttggcacgc
     3961 cgcttgctgg taagaaggcg aaccgaagta aaaaaaacca cggtcaaata cattacgggt
     4021 ttacgtttgc ggcggatgcc gcaaatttcc aatcaaactt aaacattttc aaacaggagt
     4081 catccaaatg aaagcaatcc aaaaaggttt caccctgatc gagctgatga tcgtcatcgc
     4141 catcgtcggt atcttggcag ccgtcgccct gcccgcatac caagactaca ccgcgcgcgc
     4201 ccaaatgtcc gaagccctga ctttggcaga aggtcaaaaa tccgcagtgg tcgagtatta
     4261 ttccgacaac ggcacattcc cgaacagcaa tacttccgca ggtattgctg cctctaacga
     4321 gattaaaggt aagtatgtgg catcggttaa ggttgaaggt aatgcctctg ttgcttctat
     4381 taccgctacc atgaactcta gtaatgtgaa taaggacatc aaaggtaaaa ccttggtact
     4441 cgtcggcaaa caaaactccg gctcgttctc ttgggaatgt aaaaaaggtt ctgtggacga
     4501 aaaattcttg ccatctacct gccgcaccaa ataaggacaa tgaccgggtt tgacccggtc
     4561 gtgaaaacaa atgccgcccg aaccgctcgg gcggcatttt ctattcccct ttgccgctgt
     4621 agggctgtag ggtgaggcgt aggtgcgtag ggtgggctgt agggtgggct tcagcccacc
     4681 attccaccaa cccaaccaac tccaccacat caaggatttt tggcgaaccg ttgggattcg
     4741 gcgggtttcg agtttcggcg gggattggcg gaatggttgg tttgggttgg gtgggaatgg
     4801 ttggattggc ggaatggtgg gctgaagccc accctacgaa acaccctacg aaacacccta
     4861 cagcctacac atcagcccta cgccctccct acccatttcc ctgcaaatcc cctaaaaatc
     4921 ctcccgcacg ccctgaaaac aaggaataat acaggtttcc gcccatcggg ctaaaaaggg
     4981 gataaatatg tcaaattacc ggagggactt cacgcgcggg gcgcactatt ttttcacatt
     5041 ggctttgcaa gacaggacga aggattacct gatacggcat atcgacaggc tgcgcctcgc
     5101 ctatcgtgaa acgaaaacac gttatccgtt tgaaacggcg gcaatttgta ttttgccgga
     5161 tcacctgcat ttcattatgc ggctgccgga aggcgaccac gattacccga tacggattgc
     5221 atatttgaaa acccggtttt cccaactcat ccccaaagag tgccgcgccc ccaacgggag
     5281 taaaacaaaa aaacgcgaag ccggcatttg gcagcgcagg ttttgggaac acctgatccg
     5341 ggacgaacaa gatttaaaca accaccggga ttatatttat ttcaaccccg tgaaacacgg
     5401 gtatgcggcg agtgtgaagg aatggcgtta ttcttccttc ttcaaagacg ttgaaaaagg
     5461 catttaccct ttggattggg gagggtaaag ctcccgccgc cgccaaaatc cgcaggacgg
     5521 acgcggggca gatgagtcgg gcgggacgta gggtgaggcg taggtgcgta gggtggatgt
     5581 gtagggtggg cttcagccca ccaatccaac cacctccgcc aatccaacca ttcccaccaa
     5641 cccaaaccaa ccattccgcc aatccccgcc aaaaaccgaa accgccgaat cccgacggtt
     5701 tgccaaaaac ccttgatgcg gtggaattgg cgggattgga tgagttggtt tggcagaatt
     5761 ggtgggctga agcccaccct acagcccacc ctacaacccg ccctaagtcc cacctacgtt
     5821 ccatccgacg catccgccct acggttccgg attcccctgc gaaccgcccc gcgcacgaaa
     5881 actgctttac gcggcgtttt tcgcgttttg ctgttttgct ataatccgcc ctttttaagg
     5941 acgggtgcgg tatgagtctt tacgctttgc tcttgattgc gctggggatg tcgatggatg
     6001 cgtttgccgt cgcgctggca aagggtgcgg cggtcaggat gcctccgcgc aaaattgcag
     6061 caacagcctt ggtgttcggc acggttgagg cactcacgcc gctggcaggc tgggtaggcg
     6121 gtttttatgc caaaccgttt atcagcgaat gggaccattg ggcggctttc gtcctgctgg
     6181 gcgggctggg tctgaaaatg atgcgcgaag ggctgtccgg caaggcggaa gatgtacgcg
     6241 aaagcaaacg ggaaagccta tggatgacgg ttttgactgc ttttggaacc agcatcgact
     6301 ctatgatcgt cggggtgggc ttggcgttta tggaggtaaa catcgccttt gccgccgccg
     6361 tcatcggcat ggcgacgacg gtgatggtta cggtcgggct gacggcggga agggctttgg
     6421 gcgtattgtt cggcaaacgc gccgagtttg ccggaggttt ggtgttgatt gccatcggta
     6481 catggacgct cttgtcgcac ttggggctga tcgggtaagg actgccaaaa aatgctgtct
     6541 gaagcgttgt ttgaccgttt cagacggcat tttttatttt ttgggcgtag ggcgaatccg
     6601 taaggcggga tgtaaggtgg gatgtagggt gggcttcagc ccaccaatcc caccacctcc
     6661 gccgctcccg ccaaatccgc cattcctcgc cgaaaaccga aaccgccgaa tcccgacggt
     6721 ttgccaaaaa cccttgatgc ggtgggaatg gtgggaacgg tgggattggc ggaggagatg
     6781 ggattggtgg gctgaagccc accctacgcc cactgttttt gattttttgt ttttgcgaga
     6841 atggcgggat tttggcttac tgtttttgtg aaaatggcga aaccgcgcaa atgctgatgc
     6901 cgtctgaaac actgtttgat tgtttcagac ggcatttttt gttcacatcg atcaagccgc
     6961 gttgtcgaaa ccgctgtggc gcaagagtgc gtctatgcgc ggttcgcgtc cgcggaaggc
     7021 tttgaaggat tctgccgcgc tgcgcgatcc gccgacggcg aggatttcct gccaaaagcg
     7081 tttgcctgtg gcggcgacat cgtcgctttc ttcaaaggcg gcgtatgcgt ccgcgctcaa
     7141 tacttccgcc cacgcgtagc tgtaatagcc tgcggaatag ccgcctgcga agatgtggcc
     7201 gaagctcaag gcgaagcggt tgtattcggg cggttggatg acggcgactt ctttgcgcac
     7261 gctgtctaaa acctgttgcc agtttttcag acggccttcg tcgtcttcgc tgtaaatcat
     7321 catatcgaag agggcgaact ccatctggcg gacgaggaac atgccgcgtt ggaagttttt
     7381 ggcggcgagc attttgtcga agagttcttt cggcaggggc tcgccggttt cttcgtgggt
     7441 ggacatttgc gccaatacat tgtattccca aacgaaattt tccataaact ggctgggcag
     7501 ttcgaccgcg tcccattcta cgccgttgat accggacacg cccagttcgt ccacttgggt
     7561 aagcaggtgg tgcaggccgt gtccggtttc gtggaagagg gtgaggattt cgtcatggct
     7621 caagcgggct tctttgccgc cgacgggcgg ggtgaagttg cagacgaggt aggcggtggg
     7681 cagttgcagc gtgccgtcgg cgaagcggcg gcggcctttg tagtcgttca tccatgcgcc
     7741 gccgcgtttg ccttcgcgtg cgtacaaatc catataaacg ccgcctatgg tttcgccgtt
     7801 ttgttgcaat tcaaaatagc gcacgtcttt gtgccagacg gggacggttt tttcggtaaa
     7861 tccgatgccg tagagttttt tgatttgggc gaacagtccg tttaatactt tgccgacggg
     7921 gaagtatttt ttgacttcgg tttcgctgaa tgcgtatttg gcttcgcgca gtttttcgcc
     7981 ggcgtagccc aagtcccacg gttgcaaatc ggcgagaccg aggctttcgc gggcgaaggc
     8041 tttgacttcg gcgaggtctt tttcggcgta gggtttggcg cggcgggcga ggtcgtgcag
     8101 gaagtttaaa acttgttcgg gcgtgtccgc cattttggtt gccagcgaca attcggcgta
     8161 gtttttgaag ccgagcagtt tggcggtttg cagggcgttt gcgagcgtgc ggtcgatgtt
     8221 ggcggtgttg tcgaatttgc cgtcgtctga cagttcgctg gcgcgggtaa cgtaggcgcg
     8281 gtagatttgt tcgcgcagtt cgcggttgtc ggcgtattgg atgacggcga ggtagtgcgg
     8341 aatctgcaag ccgattttgt agcctgtttt gctttcgctt tgcgcggcgg cggcaaacat
     8401 ggcgagcgag tctttgggaa tgccggcaag cggtgcggca tcgtcaaagt aaatgccgaa
     8461 cgcgtcggtc gcgtctagga cgttttggga gaatttggcg gaaagttgcg cgccttcggt
     8521 ttgcagtttt gccagttctg cctgctgttc gggcggcagt tccgcgccgc taaggacgaa
     8581 atcgcgcagg tcgtggttga gcttggtttt ttgtgcgggg gagagggtgt cgaattcggg
     8641 ggaatttttg atggttttga agcggttgta cagctcgatg tcttgtccga tttcggtgaa
     8701 gaagacggtg atttcgggca tcagttcgtt atagacggcg cgcagttcgg gcgtatcggt
     8761 gacggagttg aggtgcgaca ccacgcccca aatcctgccg acgcgttcgg tgatgccggt
     8821 caggggttcg acagtgtttg cccagccggt gtgcgtttgg gctttgatgg cggcgatttg
     8881 ttcgcgcgct tcggcgatgg cggtttgcag ggcgggtttg atgtcttcgg ttttgatttg
     8941 atcaaaacgg ggttcttcgc ccaaatggag cagtgcgttg tcagtcataa gatgggtttc
     9001 ctttgcgtgt tgtgcttcag acagcatatc ggcaatatgc cgtctgaagc cggaaatggg
     9061 gtcggtcggg gaaagttaaa gtgtttcgga gccggcgggg taatttaaca ggcggtattg
     9121 tacgcgcaaa aattcgtcca taaccgcctg ttggatttcc agccgctttg aaacggggga
     9181 ggcgaagcgg acgatgatgc ggtatgcctt gtcatcgtac ggcacgcggg taacgcgcgg
     9241 tctggcggcg ggcgtgataa acagtttttc cgcctgcacg ttttccaaat gccgttggat
     9301 ggcggggatg tagggcgcgc acaagggctc gagtacggct ttcagacggc atacggcttc
     9361 atccgaatcc aaatggatgg ggacggggat ttcgaccgta tggatgacat agtcgcccaa
     9421 aatattgtcg cggcgcacgg ggtggctcaa caacaggctg ttggggaaag aaacggtggt
     9481 tcccgcaagc tgtccgacca aggggttcgg accgacctgc atcatcagcg tgttcaacag
     9541 gttgatgtcg accacgcgcc cgcgcaggcc gttgatttcg atatagtcgc cgaccgagta
     9601 ttgctgggtg gcagacctta aaatactgcc cgacagacac ataatcagtt ccttcgtcgc
     9661 cacgacgacc gccgccgcca ccgcaaacat cgacaaagcc agcgtttgga tttgcgccga
     9721 ccagataaat gccagcgaaa acagcaccaa aagcagcgtt atattgcggc tggcaaccaa
     9781 aaaccgccgc ttgctttcga tgccgaaatc cggatgccgt ttgaagtgga tattcaacag
     9841 aagggcgcac gccagcagca aagccgcaac cgccgccacg gattcgaccg cctccgcacg
     9901 tatcgggacg gcaccgagcc aagtgtccaa cctattccat atttccattt ccgcgcccct
     9961 gtccgaaaag tatagggtgt agattttagt ggcaaaaaaa cgcttttgcc actttacggg
    10021 aaaatccccg acctgatgcc ggaggcaaac gtaccgtttc atcttttccc ggcggcacac
    10081 tgatgcggat aacgctgaat taggcggcaa aatcggctat aatacacaat tatatatgcc
    10141 gtcccgcccg ctttttccac aaggtgcgga cttgttttgg cggcatcgca aaatcttttc
    10201 aaaatccggc aaaaaatatg actgaacaaa aacacgaaga atacggcgcc gacagcattc
    10261 aggtgctcga aggcttggaa gcggtacgca aacgccccgg catgtacatc ggcgacacgc
    10321 aggacggcag cggcctgcac catatggtgt tcgaagtatt ggacaacgcc attgacgaag
    10381 cactcgccgg acattgcgac aaaatcacgg taacgataca cgccgaccat tccgtcagcg
    10441 tcgccgacaa cgggcgcggt atgcccaccg gcatccaccc gaaagaagga cgctccgccg
    10501 ccgaagtcat catgaccgta ttgcacgcgg gcggtaaatt cgacaacaac agctacaaaa
    10561 tctccggcgg cctgcacggc gtgggcgtgt ccgtcgtcaa cgcgctgtcc gactgggtaa
    10621 cgctgaccat ctaccgcgac ggcaaagaac acttcgtccg cttcgtgcgc ggcgaaaccg
    10681 aagagccgct gaacgttgtc ggcgattccg ataaaaaagg cacgaccgtg cgcttcctcg
    10741 ccagtacgga aaccttcggc aacgtcgaat acagcttcga catccttgcc aaacgcatcc
    10801 gcgaactttc gttcctgaac aacggcgtgg acatcgaatt gaccgacgag cgcgacggca
    10861 aacacgaaag cttcgccctt tccggcggcg tggctggatt cgtgcaatac atgaaccgca
    10921 aaaaaacgcc cttgcacgaa aaaatcttct acgcgttcgg cgagaaagac ggcatgagcg
    10981 tcgaatgcgc aatgcaatgg aacgacagct atcaggaaag cgtgcagtgc ttcaccaaca
    11041 acatccctca gcgcgacggc ggtacgcacc tgaccgcgtt gcgccaagtg atgacgcgca
    11101 ccatcaacag ctacatcgaa gccaacgaag tcgccaaaaa agccaaagtg gaaaccgccg
    11161 gcgacgatat gcgcgaaggt ttgacctgcg tgttgtccgt caaactgccc gaccccaaat
    11221 tttcgtccca aaccaaagac aaactggttt ccggcgaaat cggtcccgtc gtcaacgaag
    11281 tcatcagcca agccctgacc gacttcctcg aagaaaaccc tgccgaagcc aaaatcatca
    11341 ccggcaaaat cgtcgatgcc gcccgcgcgc gcgaagccgc ccgcaaagcc cgcgaaatca
    11401 cccgccgcaa aggcgtgatg gacggtttgg gcctgccggg caaactcgcc gactgccaag
    11461 aaaaagaccc cgcattgtca gaactttatc tggtcgaggg cgattctgca ggcggttccg
    11521 ccatgcaggg gcgcgaccgc aaattccaag cgattttgcc gcttaagggc aagattttga
    11581 acgttgaaaa ggcgcgcttt gaaaaaatgc tcgccagtca ggaagtagcc gctttgatta
    11641 ccgcgttggg cgcgggcatc ggcaaggaag aattcaatgc cgagaagctg cgttaccacc
    11701 gcatcattat catgaccgat gccgacgtgg acggcgcgca catccgcacc ctacttttga
    11761 ccttcttcta ccgccaaatg cccgaactgg tcgagcgcgg ctacatctat atcgcccagc
    11821 cgcctttgta taaagcgaaa tacggcaaac aggagcgtta cctcaaagac gaactggaaa
    11881 aagaccaatg gctgctcggt ttagccttgg aaaaagccaa aatcgtttca gacagccgca
    11941 ccatcgaagg cgaagagctt gccaataccg ccaaacaatt cctgttggcc aaaaccgtca
    12001 tcgaacagga aagccgcttc gtagacgaac tcgtcctgcg cgccatgcta cacgcgtcgc
    12061 ccattgattt gacgtcgtct gaaaacgccg ataaagccgt tgccgaactt tccggtctgc
    12121 ttgacgaaaa agaagtcgcc ctcgaacgca tcgaaggtca tgaaggacac cgattcatca
    12181 aaatcacgcg caagctgcac ggcaacgtca tggtcagcta catcgaaccc aagttcctca
    12241 acagcaaagc ctaccaaacc ctcacccaaa ccgccgccgc gctcaaaggc atggtcggcg
    12301 agggtgccaa gctttacaaa ggcgaaaacg ggtacgacgc ggacagcttt gaaaccgctt
    12361 tggacatctt gatgagcgtt gcccaaaaag gtatgtccat ccaacgatac aaaggcttgg
    12421 gcgagatgaa ccccgaacag ctgtgggaaa ccacgatgga tcccgccgtg cgccgcctgt
    12481 tgaaagtgcg catcgaagac gccatcgccg ccgacgaagt gttcgttacg ctgatgggcg
    12541 acgaggtcga gccgcgccgt gcctttatcg aaaacaacgc gctgattgcc caaaatatcg
    12601 acgcataagt gccgttttaa aaaaggagac gggcatcgtg ccgcgtctcc ttttggtttg
    12661 tcaaacggaa gccatgccgt ctgaaaccgc ctttcggagc aaaaccatga tcagcattt
//
LOCUS       DAWKST010000053        12712 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.053, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000053 DAWKST010000000
VERSION     DAWKST010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12712
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.053"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..1722
                     /locus_tag="WJL87_000996"
     CDS             <1..1722
                     /locus_tag="WJL87_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ7799437.1"
                     /translation="LYTKLTRKRQEIFFNQTLAFDEIDRLFDAKAFSKFSRHTADGKQ
                     AVGEIKRHSDGTPAENLIIKGNNLIALHSLATQFKGKVKLIYIDPPYYFVKKKQQDSF
                     GYNTNFKLSTWLTFMKNRLSIAKELLTDDGIIVISIDDDGNAYLKILLDEIFGFENFI
                     GNLPTIMNLKGNNDEYAFAGTHEYTLVFAKNKDKSTFYEFPIDEDNFLEKWEEDEIGF
                     YKKGAPMRATGTEDKREDRPEMFYPFLVKNNTVSTITDEEFSQIYNKDLEVFNDDFIQ
                     KLKEKYENLGYNFILPIADKQWGRWRWGYSIKNKARLQTDIIVSQSKNGISLYKKQRP
                     ELDDLPTKKPKTIFYKPEYSSGNGTEQMKNLFGEKAFKNPKPEELIQDFITITTNESD
                     IVLDYHLGSGTTAAVAHKMNRQYIGIEQMDYIETLAVERLKKVIDGEQGGISKAVNWQ
                     GGGEFVYAELAPFNETAKQQILACEDSDGIKTLFEGLCERYFLKYNVSVNEFSQIIQE
                     PEFQSLALDEQKQMVLEMLDLNQMYVSLSEMDDEQFAGCLNDDDKALSREFYQSAKNQ
                     AEKKDGE"
     gene            1712..4486
                     /locus_tag="WJL87_000997"
     CDS             1712..4486
                     /locus_tag="WJL87_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ7799438.1"
                     /translation="MANNKTLFDWVEDRKSTLEEIEQTDFFALPEFVERNLKYPFFEW
                     QKSALENFVIFDRASKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIDPAHAKFLFTEKILQGDTVIPIRKVEMFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHKLNLVMLGDEAHHLNAQTKGKKQDELDLEVELKA
                     NAGNAEIERKGWEHMVLELLLNKNGNPGQNVLLEFTATLPENADVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSSTLNKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYSAFLNWAENVQADDFEFLDTFSGSLNNSDNANEQGKTRTEQALKFMQENG
                     FEFAHLANWVKQNYQKHNVIITNSETNKNKTEKTDSETEKLLNNLEAADNPIRAIFTV
                     DRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEG
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYISELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFADNKDFRELLIWANKKIPNPNAKSNNADSLQANPQTLSFQIHGNQLLQETQ
                     FTADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFDRLQSKLN
                     IQNRNELQNNLLKDWQIEFLGLGQDKQVRPDDKLAGCLKILEMVEKHLNESDMPFIGT
                     KEFTPKKLWEIFGTPKQKWVKKDDVKTTIATQNDWYVMDNFAGTGLEEALIQFISARL
                     GDLKSKYDVHLIRNEEVFKLNNFADGEGFMPDFILLLKDKQKSSSNGVNDFLHYQIFI
                     EPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTES
                     FPLGAASLEK"
     gene            complement(4635..5807)
                     /locus_tag="WJL87_000998"
     CDS             complement(4635..5807)
                     /locus_tag="WJL87_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ7799439.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            6097..6543
                     /gene="iscR"
                     /locus_tag="WJL87_000999"
     CDS             6097..6543
                     /gene="iscR"
                     /locus_tag="WJL87_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ7799440.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            6572..7786
                     /locus_tag="WJL87_001000"
     CDS             6572..7786
                     /locus_tag="WJL87_001000"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ7799441.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WEAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILISVMWANNEIGVV
                     QDIPAIGEICRERKIVFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYINGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            8047..8433
                     /gene="iscU"
                     /locus_tag="WJL87_001001"
     CDS             8047..8433
                     /gene="iscU"
                     /locus_tag="WJL87_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ7799442.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            8520..8840
                     /gene="iscA"
                     /locus_tag="WJL87_001002"
     CDS             8520..8840
                     /gene="iscA"
                     /locus_tag="WJL87_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ7799443.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(8800..9021)
                     /locus_tag="WJL87_001003"
     CDS             complement(8800..9021)
                     /locus_tag="WJL87_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HEZ7799444.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKRGSFRRSRRSL"
     gene            9103..9603
                     /gene="hscB"
                     /locus_tag="WJL87_001004"
     CDS             9103..9603
                     /gene="hscB"
                     /locus_tag="WJL87_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ7799445.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            9701..12451
                     /gene="gyrA"
                     /locus_tag="WJL87_001005"
     CDS             9701..12451
                     /gene="gyrA"
                     /locus_tag="WJL87_001005"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ7799446.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLAHPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            12608..12712
                     /locus_tag="WJL87_001006"
                     /pseudo
     CDS             12608..12712
                     /locus_tag="WJL87_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         3855 a         3151 c         2809 g         2897 t
ORIGIN      
        1 ttatacacta aattaacccg aaaaagacaa gaaatctttt ttaatcaaac gcttgctttt
       61 gatgaaattg accggctttt tgacgcaaaa gcattctcaa aattctctcg ccacaccgca
      121 gacggcaaac aagcggttgg cgaaatcaaa cgacattcag acggcacacc agccgaaaat
      181 ctcattatca aaggcaataa tctgattgcc ctgcattcgc ttgccacgca gtttaaaggt
      241 aaagtgaagc tgatttatat tgatccgcct tattattttg ttaaaaagaa acaacaagat
      301 agttttggtt ataacactaa ctttaaatta tctacttggc ttacatttat gaaaaatcgt
      361 ttatcaattg ccaaagaatt attaactgat gatgggatta ttgttatttc cattgatgat
      421 gacggaaatg cttatttaaa gattttgtta gatgaaattt ttggatttga aaattttatc
      481 ggaaatttac ccactattat gaatttaaaa ggtaataatg atgaatatgc ttttgcaggc
      541 actcacgaat acacactagt ctttgctaaa aataaagata aatctacttt ttatgaattt
      601 ccaatagatg aagataattt tttagagaaa tgggaagaag atgaaattgg gttttataaa
      661 aaaggagctc caatgcgagc aacaggaacc gaagataaaa gagaagatag acctgaaatg
      721 ttctatcctt ttttggtaaa aaataacaca gttagtacaa ttacagatga agaattttct
      781 caaatctata ataaagattt agaagtattc aatgatgatt ttattcaaaa actaaaagag
      841 aaatacgaaa atcttggata caattttatt ttacctattg ctgataaaca atgggggcgt
      901 tggcgttggg ggtattcaat aaagaataag gcaaggctgc agacggatat tattgttagc
      961 caatctaaaa atggaatttc cttgtataaa aaacaaagac ctgaattaga tgatttacct
     1021 acaaaaaaac ctaaaactat tttttataaa cctgaataca gtagtggtaa tggaactgaa
     1081 cagatgaaaa atctttttgg tgaaaaggca tttaaaaatc ctaaaccaga agaacttatc
     1141 caagatttta ttaccatcac aaccaacgaa tccgacatcg tcttagacta ccatcttggt
     1201 agtggcacaa ccgccgccgt tgcccacaaa atgaaccgac aatatatcgg tattgaacaa
     1261 atggattata ttgaaacgct tgccgttgaa cgcttgaaaa aagtgattga tggcgagcaa
     1321 ggcggtattt ccaaagccgt gaattggcaa ggtggtggcg agtttgttta tgccgaactt
     1381 gccccattta acgaaaccgc aaaacaacaa attttggctt gcgaagattc agacggcatc
     1441 aaaacgctgt ttgaaggttt atgcgaacgc tatttcttga aatacaacgt cagcgtaaat
     1501 gaatttagtc aaatcattca agagcctgaa tttcaatcct tggcattaga cgaacaaaaa
     1561 caaatggtgc ttgaaatgtt ggatttaaat caaatgtatg tttcattatc cgaaatggat
     1621 gatgagcaat ttgcaggttg cttaaatgat gacgataaag cgttaagccg tgaattctat
     1681 caatcagcaa aaaatcaagc ggagaaaaaa gatggcgaat aataaaacgt tatttgattg
     1741 ggtggaagac cgcaaatcaa cgctggaaga aatagaacag acagattttt tcgcgctgcc
     1801 tgaatttgta gaacgcaatt taaaatatcc gttttttgaa tggcaaaaat cggctttaga
     1861 aaactttgtg atttttgacc gcgcttcaaa attaaaggat ttccctgaca tcaaaaaccg
     1921 ccctacccat ttgctgttca atatggcaac aggtgctggc aaaacgatga tgatggcggc
     1981 gttgattttg tattattttg aaaaaggtta tcggcatttt ttgtttttcg tcaatcaaaa
     2041 taatatcgtg gataaaacgg aaaataattt tattgacccg gcgcacgcaa aatttttatt
     2101 taccgagaag attttgcaag gtgatacggt aattcctatt cgcaaagtgg aaatgttcag
     2161 cccacattca gacggcattg aaattaaatt taccagcatt caaaaactgt ataacgatat
     2221 ccacaccgag cgagaaaacc aaaccacatt ggcagatttg cacaaattga accttgtgat
     2281 gctgggcgat gaagcacacc atttaaacgc acaaaccaaa ggcaaaaaac aagacgagtt
     2341 ggatttggaa gtggaattaa aagccaatgc aggcaatgcc gaaattgaac gcaaaggctg
     2401 ggagcatatg gttttggaat tgttgctcaa taaaaatggc aatcccggcc aaaatgtgct
     2461 gttggaattt accgccacgc tgcctgaaaa tgctgacgtg caacaaaaat atgcggacaa
     2521 aatcatcaca aaatttggct taaaagagtt tttgcaaaaa ggttatacca aagaaatcaa
     2581 tttggtatcc agtacgctga ataagaaaga gcgggtgttg cacgctttat tatttgcttg
     2641 gtatcgccat caaatcgcgt tgaaatacgg cattgccaat ttcaagcctg tgatgttgtt
     2701 tagaagtaag acgattgatg aatcaaaagc ggattattcg gcatttttaa attgggcaga
     2761 aaatgtacaa gcggatgatt ttgagttttt agatacgttt tcaggcagcc tgaataatag
     2821 cgacaacgcc aacgaacaag gcaaaacccg aaccgaacaa gccctaaaat ttatgcagga
     2881 aaatggcttt gagtttgcac atttggcaaa ttgggtgaaa caaaattatc aaaaacataa
     2941 tgtgattatt accaactccg aaaccaacaa aaacaaaact gaaaaaaccg acagcgaaac
     3001 cgaaaaattg ctaaacaatt tggaagcggc tgataatcca atccgtgcca tttttacggt
     3061 agatagatta accgaaggtt gggacgttct gaatttgttt gatattgtgc gtttgtatga
     3121 agggcaaaat ggcggtggtt caaataaaaa atcaggcaaa acggctgccg ctaccgtatc
     3181 ggaaaagcag ttgattggtc gtggcgtgcg ttattttcca tttgcgtttg aaggtaaaca
     3241 gccgaataaa cgcaaatttg acaacgacat gcaacacgaa ttgcgtatct tggaagaatt
     3301 gttttattac acgcacgatg agcaatcccg ctatatttca gaactgaaaa atgagttgcg
     3361 aaaagacggt tatttgcctg aaaaagacga tgataaggtg ttggcaacat ttaaactcaa
     3421 atctgaattt gccgataata aggattttag agagttgtta atttgggcga ataaaaaaat
     3481 ccccaaccca aacgccaaaa gcaataatgc agacagcctg caagccaatc cgcaaacgct
     3541 ttcattccaa attcacggca atcaattgtt gcaggaaacg caatttacag ccgatgaaaa
     3601 tgatgaaaca gcccgacaaa tcggcacaca aaataatttt actcaaacca taaaaatgag
     3661 tgaaatggag cggcacattt tcaataaagc attacatatc aaagggaaaa atagtcaatc
     3721 tttattccat tttgaccgcc tgcaaagcaa actcaacatt caaaatcgca atgaattgca
     3781 aaataacttg ttgaaagact ggcaaattga atttttagga ttagggcaag acaaacaggt
     3841 tcgcccggat gataaacttg caggctgcct gaaaatcttg gaaatggttg aaaaacatct
     3901 gaatgaaagt gatatgccgt ttatcggtac aaaagaattt acacctaaaa aattatggga
     3961 aattttcggc acgccaaaac aaaaatgggt caaaaaagat gatgtaaaaa cgactattgc
     4021 cacgcaaaat gattggtatg tgatggataa ttttgctgga acgggtttgg aagaagcgtt
     4081 aattcaattt atttcagcgc gtttgggcga tttgaaatct aaatatgacg ttcatttaat
     4141 ccgtaatgaa gaagtgttta aattgaataa ctttgccgat ggtgaaggat ttatgccgga
     4201 ctttatttta ttgctgaaag ataaacaaaa atcttcttcc aatggtgtga acgacttttt
     4261 gcattaccaa attttcattg agcccaaagg tgagcattta gtggaaactg atggatggaa
     4321 agaagaattt ttagaagcca tcactgtgga atatgggatg gataagatcc tgcaaaaaga
     4381 tacaccgcat tatcgtttga tcggtttacc gttttttact gatcatcaga aaaatggaca
     4441 atttacggag tcattccctt taggtgcggc atcgcttgaa aaatagagta gtgcattgca
     4501 ggcaaccccg tttgacaaaa cttcctttac aaaagggcgt tttgtcagat atttaatcaa
     4561 cacattatta aaatacagcc aaattttaat gccgtccgaa ccctgtgttc agacggcatc
     4621 gtatttttca gtatctaaac cgtttcccta ccccaatctt taccccttaa aatcgaggca
     4681 tcgacatctt gaatatcgcg gtgtcccgta aacgccatag atatatccat ttctttatat
     4741 agaatttcca gcgcacgggt tacgccttct tcgccatacg cgcccaagcc atacaggaac
     4801 gcccgaccta tcattgtacc tttcgcgccc aaagcccacg ccttcaaaat atcctgaccg
     4861 ctgcggatgc cgctgtccat ccaaacttcg atgtcgctgc ccactgcgct gacgatgtcg
     4921 ggcaaggctt tgatggcaga cacggtatcg tcgagctgtc ggccgccgtg gttggaaaca
     4981 atcaatgcgt ccgcgccgct tttcgctgct ttttccgcgt cttcgggttc cataatgcct
     5041 ttgataatca gcttgccgcc ccacaaatct ttaatgcgcg ccacatcgtc ccagctcagg
     5101 cgcgggtcga attgttcgga agtccatgaa gacagcgaag acaaatcgcc gacgttcttc
     5161 gcgtgtccga cgatattgcg gaacgtgcgg cgttccgtgt tcagcatttt catgcaccat
     5221 tccggtttgg tcgccagatt gattaaattg gcgatggtcg gtttcggcgg cgcggacagg
     5281 ccgtttttga tgtctttgtg gcgttgaccc aaaacctgca aatcggcggt caataccaat
     5341 gccgaacatt tggcatcctt cgcgcgctta atcaggtttt ccataaactc gcggtcgcgc
     5401 atcacataaa gctgaaacca aaacggcgca ctggtgttct cggcaacgtc ttcaatcgag
     5461 cagatggaca tggtggacaa cgtaaacgga ataccgaact tctccgccgc ccgcgccgcc
     5521 aaaatttcgc cgtctgcgtg tgccatgccg gtaaaacccg tcggcgcaat cgccaccggc
     5581 attttcacat cctgcccgat cattttagtc tcaaggctgc gtccctccat attgaccaat
     5641 actttttgac ggaagcggat gtctttgaaa tccgaagtat tttcccgata agtcgtttcc
     5701 gtccacgaac ccgaatcgat gtaatcgtaa aacatacgcg gcattttgcg cttggcaacg
     5761 cggcgcaagt cttcgatgca ggtcattttg ctcaaatcac gtttcatttg tcgccccctg
     5821 aatacctgaa taactttata tgaaatagat aatgtatatc aatattgatt ataaggcaaa
     5881 tcatttcaac atttactgca cccgccgccg ctcccacttc aagcggcata aggtttagaa
     5941 ttcaaaaata atatattaaa atcaaaatat taaaaatcaa tcaatctgcc gatttaaaca
     6001 gccaatcgcg caatccgccc ccatacttga ctgaaacact cagatattgg acaattccgc
     6061 ccactataaa aaaagccgac acgggcaacc accaccatga gactgaccac caaagggcgt
     6121 ttcgccgtta ccgctatgct ggatttggcg atgaacgcgc aaaccggcgc cgtcaaactc
     6181 agtgccatca gcgaacgcca aaacatatcc ctctcctatc tcgagcaatt gttcggcaaa
     6241 ctccgccgcg ccggacttgt tgaaagcctg cgcgggcccg gcggcggcta catcctcgcc
     6301 gcaccggcgg cacgcatcaa catcgcccaa atcatcgccg ccgccgaaga ccggctggac
     6361 gcgacccaat gcggcagcaa agccaactgc caccacggcg cgccctgcct gacgcacgac
     6421 ctttgggaga atttaaacaa aaccatcaac gactacctcg gcagcgttac cctgcaaagc
     6481 atcatcgaac agaaaaacaa cggcgacggc agccgcgtcg tccaatttac acacatccat
     6541 taaataacac ccgaaaaaga aagggcaaac catgaccgtc aaaacccccg tttacctcga
     6601 ctacgccgcc accacccccg ttgacaaacg cgttgccgaa aaaatgattc cctatctgac
     6661 cgaaaccttc ggcaaccccg cctccaacag ccacgcattc ggctgggaag cagaagaggc
     6721 cgtcgaaaaa gcccgcgccg acatcgctgc cctgattaat gccgacccca aagaaatcgt
     6781 cttcaccagc ggcgcgaccg agtccgacaa cctcgccatc aaaggcgcgg caaacttcta
     6841 caaaaccaaa ggcaaacacc tcatcaccgt caaaaccgaa cacaaagccg ttttagatac
     6901 aatgcgcgaa ctcgaacgcc aaggttttga agtaacctac ctcggcgttc aagaaaacgg
     6961 tttgattgat ttggaagaac tcaaagccgc catccgcgac gacaccatcc tgatttccgt
     7021 aatgtgggca aacaacgaaa tcggcgtggt gcaagacatt cccgccatcg gcgaaatctg
     7081 ccgcgaacgt aaaatcgtct tccacgtcga tgccgcccaa gcctgcggca aagtgcctgt
     7141 cgatgtcgaa gccgccaaaa tcgacctgct ctcgatgtcc gcgcacaaag tgtacggccc
     7201 caaaggcatc ggcgcgctgt acgtccgccg caaaccgcgc gtccgcctcg aagcccaaat
     7261 gcacggtggc ggccacgaac gcggcttccg cagcggcaca ttaccgaccc accaaatcgt
     7321 cggtatgggc gaagccttcc gcatcgccaa agaagaactc gaacaagata tggcgcacta
     7381 ccgcaaactg cgcgacatct tcctcaaagg catcgaaggc atcgaagaag tctatatcaa
     7441 cggcgacctc gaacaccgcg ccccgaacaa cctgaacgtc agcttcaact tcgtcgaagg
     7501 cgaaagcctg attatggcag tgaaagaact cgccgtatcc agcggctccg cctgcacctc
     7561 cgcctcgctc gaacccagct acgtcctgcg cgcattgggc agaaatgacg aattggcgca
     7621 ctcgtccctg cgcatcacct tcggtcgcat gaccactgaa gaagaagtgc aatttgccgc
     7681 cgaactgatt aaatccaaaa tcggcaaact gcgcgaactg tcgccgttgt gggaaatgtt
     7741 caaagacggg attgatttga actcgattga atgggcggcg cattaaagcg taccaacatg
     7801 ccatccgaac cttcagacgg cattccaaaa acaaagcaat caagagaaaa atatgaacga
     7861 acaagattta gatttggaca acctcgacaa cctgcttgac gatttcgacg gcgttaccgt
     7921 ggaaggcggc gtcgattcgg aaaacgacga cggctgtgaa ggcggagcgt gcaaaatcta
     7981 actcaggccg tctgaaaacc ggcaaacaaa ccacttaaat catcaaaaca ttaaggaaac
     8041 cacatcatgg catacagcga taaagtaatc gaccactacg aaaatccccg caacgtcggt
     8101 actttcgaca aaaacgacga atctgtcggc accggcatgg tcggcgcacc ggcctgcggc
     8161 gacgtgatgc gcctgcaaat caaagtgaac gatgaaggca tcatcgaaga tgcgaaattc
     8221 aaaacttacg gctgcggttc cgccatcgct tcgtccagcc tgattaccga gtgggtcaaa
     8281 ggcaaaagtc tggatgacgc gctggcaatc aaaaacagcg aaatcgccga agagttggaa
     8341 ttgccgccgg taaaaatcca ctgctccatc ttggctgaag atgcggtaaa agcggccgtt
     8401 gccgactacc gcaaacgtca ggaaaacaga taaagccttt cagacggcat cgtcccgcaa
     8461 tgccgtccga accacccgcc gcttcaggtc cgtccggggc ggtacaacaa ggaagaaata
     8521 tgattaccct taccgagaat gccgcaaaac acatcaatga ctatctcgcc aaacgcggca
     8581 aaggcttggg cgtacgcttg ggtgtgaaaa ccagcggctg ctcggggatg gcgtacaacc
     8641 ttgaatttgt cgatgaagcc gatggcgacg acctgatttt cgaaggacac ggcgcgcgca
     8701 tttatatcga tccgaaaagc ctggtttatc tggacggcac gcaagtcgat tacaccaaag
     8761 aaggtttgca ggaaggtttc aaatttgaaa accccaatgt caaagactcc tgcggctgcg
     8821 gcgaaagctt ccacgtttaa ggcataaaaa cggcgggaca gtatcaaaac cgtcccgcca
     8881 ttttttcgct tcctgcctgt tgtagctgcc tttgcctttc cttttccgtt ccaccttgtg
     8941 ccggaacaaa tcggatttca ctaaggcttt taaagcattg tcgcgtattt tgcctttatt
     9001 gtgctgcact ttgccgccca tattcagtcc tttcgtttaa gaagctgcag attataaggc
     9061 aaaaacagtt ttctgccaaa atcttacatt tatcattcta ctatgtccca atatttcacc
     9121 ctcttccgga ttgaacccgc tttcgatatc gacaccgaaa acttggaaca aacctaccgc
     9181 gccttggccg cccgtttcca tcccgataaa ttcgcttcag cttccgcctt tgagcaaaag
     9241 caggcagtga tgatgtcttc caccatcaac gatgcctacc gcaccttgaa aaaccccatc
     9301 gaccgcgccg cctacctgct gaaaacatcg ggcatcgatg ccgacgcgcc ggagcatacc
     9361 gctttcgccc ccgaattcct tatgcagcaa atggaatggc gcgaaacgct gatggaggca
     9421 cgggcaggca acgaccttga atccttgaaa aatctcgaca acgaaatccg cgacgaacaa
     9481 gaaaaactgt tctgcggtct gaaacagtcg tttgcccgac aagattacga cacagccgca
     9541 caacaagtcc gccaaggcag gtttctcgac aaactccgca acgaaatttc ctcggcatta
     9601 taatccgcac cgtgtttcag acggcgtaac cgccgcaccg ttccgcgtca aaatatgcta
     9661 aaataagcaa caattttttg ccatacgaaa cattgaaacc atgaccgacg caaccatccg
     9721 ccacgaccac aaattcgccc tcgaaaccct gccggtaagc cttgaagacg aaatgcgcaa
     9781 aagctatctc gactacgcca tgagcgtcat tgtcgggcgc gcgctgccgg acgttcgcga
     9841 cggtctcaag ccggtacacc gccgcgtact gtacgcgatg cacgaattga aaaacaactg
     9901 gaatgccgcc tacaaaaaat cggcgcgcat tgtcggcgac gtcatcggta aataccaccc
     9961 ccacggcgat accgccgtat acgacaccat cgtccgtatg gcgcaaaatt tcgctatgcg
    10021 ttatgtgctg atagacggac agggcaactt cggatcggtg gacgggcttg ccgccgcagc
    10081 catgcgctac accgaaatcc gcatggcgaa aatttcccac gaaatgctgg cagacattga
    10141 ggaagaaacc gtcaatttcg gcccgaacta cgacggtagc gaacacgagc cgcttgtact
    10201 gccgacccgt ttccccacac tgctcgtcaa cggctcgtcc ggcatcgccg tcggcatggc
    10261 gaccaatatc ccgccgcaca acctttccga taccgtcaat gcctgcctgc gcctgctcga
    10321 tgcacccgac accgaaatcg acgaactgat cgacattatc caagcccccg acttcccgac
    10381 cggggcaacc atctacggct tgagcggcgt gcgcgaaggc tataaaacag gccgcggccg
    10441 cgtcgttatg cgcggtaaga cccatatcga acccataggc agaaacggcg aacgcgaagc
    10501 catcgttatc gacgaaatcc cctatcaggt caacaaagcc aagctggtcg agaaaatcgg
    10561 cgatttggtt cgggaaaaaa cactggaagg catttccgag ctccgcgacg aatccgacaa
    10621 atccggtatg cgcgtcgtta tcgagctgaa acgcaacgaa aatgccgaag tcgtcttaaa
    10681 ccaactctac aaactgactc cgctgcaaga cagtttcggc atcaatatgg tggttttggt
    10741 cgacggacaa ccgcgcctgt tgaacctgaa acagattctc tccgaattcc tgcgccaccg
    10801 ccgcgaagtc gttacccgac gtacgctttt ccggctgaag aaggcacgcc atgaagggca
    10861 tattgccgaa ggcaaagcgg tcgcactgtc caatatcgat gaaatcatca agctcatcaa
    10921 agaatcgccc aacgcagccg aggccaaaga caaactgctt gcgcaccctt ggcgcagcag
    10981 cctcgttgaa gaaatgctga cgcgttccgg tctggatttg gaaatgatgc gtccggaagg
    11041 attggctgca aacatcggct tgaaagagca aggttattac ctgagcgaga ttcaggcaga
    11101 tgctatttta cgcatgagcc tgcgaaacct gaccggcctc gatcaagaag aaattgtcga
    11161 aagctacaaa aacctgatgg gtaaaatcat cgactttgtg gatatcctct ccaaacccga
    11221 acgcattacc caaatcatcc gcgacgaact ggaagaaatc aaaaccaact atggcgacga
    11281 acgccgcagc gaaatcaacc cgttcggtgg cgacattgcc gatgaagacc tgattccgca
    11341 acgcgaaatg gtcgttaccc tgacacatgg cggctatatc aaaacccagc cgaccaccga
    11401 ctatcaggcg cagcgtcgcg gcgggcgcgg caaacaggcg gctgccacca aagacgaaga
    11461 ctttatcgaa accctgtttg ttgccaacac gcatgattat ttgatgtgct ttaccaattt
    11521 gggcaagtgt cattggatta aggtttacaa actgcccgaa ggcggacgca acagccgcgg
    11581 ccgtccgatt aacaacgtca tccagttgga agaaggcgaa aaagtcagcg cgattctggc
    11641 agtacgcgag ttccccgaag accaatacgt cttcttcgcc accgcgcagg gaatggtgaa
    11701 aaaagtccaa ctttccgcct ttaaaaacgt ccgcgcccaa ggcattaaag ccatcgcgct
    11761 caaagaaggc gactacctcg tcggcgctgc gcaaacaggc ggtgcggacg acatcatgct
    11821 gttctccaac ttaggtaaag ccatccgctt caacgaatac tgggaaaaat ccggcaacga
    11881 cgaagcggaa gatgccgaca tcgaaaccga aatttcagac ggcatcgaag atgaaaccgc
    11941 cgacagcgaa aacgcactgc cgagcggcaa acacggtgtt cgcccgtccg gtcgcggcag
    12001 cggcggtttg cgcggtatgc gcctgcctgc cgacggcaaa atcgtcagcc tgattacctt
    12061 cgcccctgaa accgaagaaa gcggtttgca agttttaacc gccaccgcca acggatacgg
    12121 aaaacgcacc ccgattgccg attacagccg caaaaacaaa ggcgggcaag gcaatattgc
    12181 cattaacacc ggcgagcgaa acggcgattt ggtcgccgca accttggtcg gcgaaaccga
    12241 cgatttgatg ctgattacca gcggcggcgt acttatccgc accaaagtcg aacaaatccg
    12301 cgaaaccggc cgcgccgcag caggcgtgaa actgattaac ttggacgaag gcgaaacctt
    12361 ggtatcgctg gaacgtgttg ccgaagacga atccgaactc tccgacgctt ctgtaatttc
    12421 caatgtaacc gaaccggaag tcgagaactg aaaatcatct cccgatgccg tctgaagatt
    12481 cagacggcat ttattttatc cctcatccgt catccagctt ctcacaatat agcggattat
    12541 agtcaattaa aaacaagggg ctgtcctaga taactaggga aattcaaatt aagttagagt
    12601 tgcccctatg agaaaaagtc gtctaagccg gtataaacaa ataaactcat tgaactgttt
    12661 gtcgcaggtg taactgcaag aacgacagca gagttagtag gcgttaataa aa
//
LOCUS       DAWKST010000054        12383 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.054, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000054 DAWKST010000000
VERSION     DAWKST010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12383
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.054"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            109..1650
                     /locus_tag="WJL87_001007"
     CDS             109..1650
                     /locus_tag="WJL87_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ7799447.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAAMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGWQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRACSDKAVSPVMPSEPQSVGEYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLAAFALGIFLLLDGGEGGK
                     RTETL"
     gene            1679..2278
                     /locus_tag="WJL87_001008"
     CDS             1679..2278
                     /locus_tag="WJL87_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ7799448.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            2275..2880
                     /gene="nadD"
                     /locus_tag="WJL87_001009"
     CDS             2275..2880
                     /gene="nadD"
                     /locus_tag="WJL87_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ7799449.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPTGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRNLASQGVSDGIPPAAARYIREHGLYEK"
     gene            2939..3325
                     /gene="rsfS"
                     /locus_tag="WJL87_001010"
     CDS             2939..3325
                     /gene="rsfS"
                     /locus_tag="WJL87_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ7799450.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            3379..3849
                     /gene="rlmH"
                     /locus_tag="WJL87_001011"
     CDS             3379..3849
                     /gene="rlmH"
                     /locus_tag="WJL87_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ7799451.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYVLKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKTWQQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE"
     gene            3924..4190
                     /locus_tag="WJL87_001012"
     CDS             3924..4190
                     /locus_tag="WJL87_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ7799452.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            4347..5030
                     /locus_tag="WJL87_001013"
     CDS             4347..5030
                     /locus_tag="WJL87_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEZ7799453.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEGSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            5154..5666
                     /gene="coaD"
                     /locus_tag="WJL87_001014"
     CDS             5154..5666
                     /gene="coaD"
                     /locus_tag="WJL87_001014"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ7799454.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            5698..6351
                     /locus_tag="WJL87_001015"
     CDS             5698..6351
                     /locus_tag="WJL87_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ7799455.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNRESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRRGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
     gene            6693..8233
                     /locus_tag="WJL87_001016"
     rRNA            6693..8233
                     /locus_tag="WJL87_001016"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            8334..8410
                     /locus_tag="WJL87_001017"
     tRNA            8334..8410
                     /locus_tag="WJL87_001017"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8368..8370,aa:Ile,seq:gat)
     gene            8416..8491
                     /locus_tag="WJL87_001018"
     tRNA            8416..8491
                     /locus_tag="WJL87_001018"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8449..8451,aa:Ala,seq:tgc)
     gene            8898..11789
                     /locus_tag="WJL87_001019"
     rRNA            8898..11789
                     /locus_tag="WJL87_001019"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            11886..11999
                     /gene="rrf"
                     /locus_tag="WJL87_001020"
     rRNA            11886..11999
                     /gene="rrf"
                     /locus_tag="WJL87_001020"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            12035..>12383
                     /locus_tag="WJL87_001021"
     CDS             12035..>12383
                     /locus_tag="WJL87_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEZ7799456.1"
                     /translation="MLYPEKMSGMAGRHPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASQQELEALPGIGPAKAKAIAE
                     YRAQNGAFKSVDDL"
BASE COUNT         3047 a         2985 c         3593 g         2758 t
ORIGIN      
        1 ttcagacggc gggaggaagg cggatacggt atgtagtcgg attgaaatat acggtatgtg
       61 gggcttttgg gtacaatcgc aacatcagtg tttcaagtgg ggaaggcgat ggatggacgg
      121 cgttgggcgg tatggggtgc ttttgccctg ctgccttcgg cttttttggc ggcaatggtc
      181 gttgcgcctt tgtgggcggt ggcggcgtat gacggtttgg cgtggcgcgc ggtgctgtcg
      241 gatgcctata tgctcaaacg tttggcgtgg acggtatttc aggcagcggc aacctgtgtg
      301 ctggtgctgc ctttgggcgt gcctgtcgcg tgggtgctgg cgcggctggc gtttccgggg
      361 cgggctttgg tgctgcgcct gctgatgctg ccgtttgtga tgcccacgtt ggtggcgggc
      421 gtgggcgtgc tggctctgtt cggggcggac ggtctgttgt ggcgcggctg gcaggatacg
      481 ccgtatctgt tgttgtacgg caatgtgttt tttaacctgc ccgtgctggt cagggcggcg
      541 tatcaggggt ttgtgcaagt gcctgcggca cggcttcaga cggcacggac gttgggcgcg
      601 ggggcgtggc ggcggttttg ggacattgaa atgcccgttt tgcgcccgtg gcttgccggc
      661 ggcgtgtgcc ttgtcttttt gtattgtttt tccgggttcg ggctggcatt gctgctgggc
      721 ggcagccgtt atgccacagt cgaagtggaa atttaccagt tggtcatgtt cgaactcgat
      781 atggcggttg cttcggtgct ggtgtggctg gtgttggggg taacggcggc ggcagggttg
      841 ctgtatgcgt ggttcggcag acgcgcgtgt tcggataagg cagtttctcc tgtgatgccg
      901 tctgaaccgc agtcggtcgg ggaatatgtg ctgctggcgt ttgcggcggc ggtgttgtct
      961 gtgtgctgcc tgtttccttt gttggcaatt gttgtgaaag cgtggtcggc cggcgaatcg
     1021 tggcgtgtgt taatggaaag tgaaacgtgg caggcggtgt ggaatacttt gcgcttctcg
     1081 gcggcggcgg tgtttgcggc ggcggttttg ggtgtggtgt atgcggcggc ggcgcggcgg
     1141 tcggcgtgga tgcgcgggct gatgtttttg ccgtttatgg tgtcgccggt ttgtgtttcg
     1201 gcgggcgtgc tgctgcttta tccgcagtgg acggcttcgt tgccgttgct gctggcgatg
     1261 tatgcgctgc tggcgtatcc gtttgtggca aaagatgttt tgtcggcttg ggacgcactg
     1321 ccgccggatt acggcagggc ggcggcgggt ttgggcgcaa acggctttca gacggcatgc
     1381 cgcatcacgt tccccctctt gaaaccagcg ttgcggcgcg gtctgacttt ggcggcggca
     1441 acctgcgtgg gcgaatttgc ggcaaccttg ttcctgtcgc gtcccgagtg gcagacgctg
     1501 acgactttga tttatgccta tttgggacgc gcgggtgagg ataattacgc gcgggcgatg
     1561 gtgctgacat tgctgttggc ggcgttcgcg ctgggtattt tcctgctgtt ggacggcggc
     1621 gaaggcggaa aacggacgga aacgttataa tgcgaaccct ttttcagagg acggcaaaat
     1681 gagcgagttg gataacatcc ttgcccataa ccggcagttt gtcgagtcgg gcgaatatga
     1741 aaaatacttt accgacaaat accccgaacg cgggctggca gttttgtcct gtatggatgc
     1801 gcggattatc gggctgctgc ccgacgcgtt gggtttgaaa aacggcgatg ccaagctgat
     1861 taaaaatgcc ggcgcgctgg ttacgcaccc gtggggttcg gtgatgcgga gccttttggt
     1921 tgccgtgttt gaactgaagg tcagagagat tatggtcatc gcccatcacg attgcggtat
     1981 gcaggggctg aatgccgaag aattcctcgg gcgcgtccgg gaaagccgga ttcccgaaga
     2041 ccgtatcgaa accctgcgtt atgccggtat cgacctcgac ggctggctga ccggtttcga
     2101 caacgtcgaa gacagcgtgc gccacacggt ggaccttatc cgtaaccatc cgctgatgcc
     2161 gcgccatatc gccgttcacg gactggtcat ccatcccgtt accggcaaac tgacgctggt
     2221 tgtggacggc agtgtttcag acggcatgga cttatcggaa ggaatggaaa catcatgaag
     2281 aaaatcggac tgttcggcgg cactttcgac ccgatacaca acgggcatct ccacatcgcc
     2341 cgcgcctttg ccgacgaaat cggcttggac gcggttgttt tcctgccgac aggcggcccg
     2401 tatcacaaag acgcagcctc cgcttccgcc gccgaccgcc ttgccatggt cgaactggcg
     2461 acggcagaag acgcgcgttt tgccgtcagc gattgcgaca tcgtccgaga aggtgcaacc
     2521 tatacttttg ataccgtcca aatcttccgc cagcagttcc catccgcgca actctggtgg
     2581 ctgatgggca gcgacagcct gatgaagctg cacacatgga aaaaatggca gatgctcgtg
     2641 cgcgaaacca atatcgccgt cgccatgagg cagggcgaca gcctgcacca aaccccgcgc
     2701 gaactgcacg cgtggctggg caagtccctt caggacggca gcgtccgcat cttgtccgcc
     2761 ccgatgcata atgtgtcgtc aacggaaatc cgccgcaacc ttgccagcca aggcgtttca
     2821 gacggcatcc cgcctgccgc cgcacgctac atccgcgaac acggtttgta tgaaaaataa
     2881 agtcaaatca gtaagaaacg gctataatgc cgtctgaaaa catcccgtta ggaaaataat
     2941 gaacgaacaa gaactgcaag acctgcaaaa aatggtcggg gtcgccgtca acgccctcga
     3001 agacatcaaa gccaaagaca tttccgttct cgaaacgcaa gacaaaactt cgctgtttgc
     3061 cagaatgatt atcgccagcg gcgacagtac gcgccaagtc aaagcactgg ccaacaacgt
     3121 tgccgtcgat ttgaaagaag ccggttttga aatcctcagc accgaaggcg acagcggcga
     3181 atggacgttg gttgatgcag gagacctcgt cgtccacgtt atgctccccg ccgtgcgcga
     3241 cttctatgat atcgacaccc tttggggcgg cgagaaaccg agtttccacg ccggaatgca
     3301 gaagccgtgg cacgcggcag actgattccc gatgccgtct gaatgttcag acggcatttt
     3361 cctgtaaggg agaaagcatt gaacatcacc gttttggcag tcggcaccaa aatgccgcgc
     3421 tgggttgatg aggccgtcgc cgaatacgcc aaacgcttcg gacgcgacgt cgcctacgta
     3481 ctcaaagaaa tcaaacccga aaaacgcggc gcgggcgtga atgccgccca aggaatggcg
     3541 gcggaagaaa aacgcatcct cgaagccata ccgcaaggcg cgttcctcgt cgtccttgac
     3601 gaacgcggca aagcaccgac ctccgtcgag ctggcggaac acctcaaaac ttggcagcaa
     3661 aacggcgaac acgtctgctt cgtcatcggc ggcgcggacg gcatgaccga ccgcctcaaa
     3721 cagcaggccc gcatgatgat gcgcctgtcc agcctgaccc tgccgcacgg gatggtgcgc
     3781 gtccttctga ccgaacaact ctaccgcgcc gcctccatcc tgcacaacca cccctaccat
     3841 cgggaataag aaggcttttg tccgcactcc caatcggtta aaatcccccc gttatttctg
     3901 aacacacagc aaaggaatcc gctatggcac gcatggtatt ctgcgtcaag ctcaacaaag
     3961 aagccgaagg catgaaattt ccgccgcttc ccaacgaatt gggcaagcgc atttttgaaa
     4021 acgtatcgca agaagcgtgg gcggcgtgga cgcgccacca aacgatgctg attaacgaaa
     4081 accgtttaag cctcgccgat ccgcgcgcgc gcgaatacct ggctcagcag atggagcagt
     4141 atttcttcgg cgacggcgcg gatgccgttc agggatacgt tccgcaataa cggttttccg
     4201 ttttgaacac aggctgtccg aaactgcttc agacggcctt taaaatacgc cggacaactt
     4261 tatcttcacg caaccggaca aattttgaca ttgggcaatg ttaaaatccc ccattattcc
     4321 caaccgcgct ttcaggagca gatgatatgc aacacgacgt ttacgactac accgcgcata
     4381 cggtttctaa aaacaccgtc ctgcagaaaa cctaccgcct gctcggattt tcattcattc
     4441 cggcagccgc aggcgcggca cttgccgcca atgccggttt caatttttac gccgccttcg
     4501 gttcgcgctg gatagggttt gccgtcgtgt tggcgttttt ctacggtatg atccacttca
     4561 tcgagaaaaa ccgttacagc aataccggcg ttaccctgct gatggtattc acattcggta
     4621 tgggcgtatt gatcggcccc gtgctgcaat acgcgctcca tattgccgac ggtgcgaaaa
     4681 tcgtcggcat tgccgccgcg atgaccgccg ccgtcttttt aacgatgtcc gccttggcgc
     4741 gccgaacccg gctcgatatg aacgcgctcg gacgcttcct gaccgtaggt gcggtcattc
     4801 tgatggtcgc cgtggttgcc aatctgtttt tgggtattcc cgcactcgcc ctgaccattt
     4861 ccgccggttt tgtcttgttc agttccttaa tgattatgtg gcaggtacgc accgtcatcg
     4921 acggcggcga aggcagccac atcagcgcgg cactgacact gtttatctcg ctttacaaca
     4981 tcttcagcag cctgctgaac atcctgttgt ctttaaacgg cgaggattga tgtcggtaaa
     5041 acgccgtccg actatgccgt ctgaaaatgc ttcagacggc attttacttt gggcatacaa
     5101 tacggatgca cacgcaccaa cacacagccg gctatgaagc cgggaggaat caaatgttac
     5161 cgaatacacc gcgccgcgcc gtttatgccg gcagtttcga tccgcccaca ttggggcatc
     5221 tgtggatgat acggcaggcg caatctatgt ttgacgaact catcgtcgcc atcggcatta
     5281 accccgacaa acgcagcacc tataccgtcg ctgaaaggca ggatatgttg tgcgatatta
     5341 ctaaaatgtt tcccaacgtc agaaccgatg tatttgaaaa ccgatttctg gtgcattatg
     5401 cccgtgaggt agatgcagga ttcatcgtgc gcggcatccg ttctgcttcg gattacgaat
     5461 acgaacgttc catgcgccac atcaacagcg acctcgcccc cgaaatatcc accgtattcc
     5521 tcatgccgcc gcgcgaaatc gccgaagtgt cgtccactat ggtcaaagga ctggtcgggc
     5581 cggaaggctg gacggaaacg atccaccgct acgtgccgca agctgtgtac gaaaaaatcc
     5641 ttgccgaaca tcaacacgaa aattgaaccc ttctattttt gcaaggcata tatggatatg
     5701 ttggaaattt tgttccgcca tcaagacttc gtcgccatca acaaaccggg cggcatatcc
     5761 gtccaccaag acagcggcga gaccggactg gcccgaacac tcgccataca gttgggcgta
     5821 gagcgtgtgt ggctgctgca ccgattggac aaacagacca gcggcatttt actgtttgcc
     5881 ctcaacagag aaagcgcgtc cgcccttagc ggacagtttg ccggcaaaag catcaaaaaa
     5941 acctatttgg cactatccga ccgcaaaccg tccaaaaagc agggttggat taaaggcgga
     6001 atggaaaaat ccagacgcgg aatgtggaag ttgacgcgca atacagaaaa tatcgccgtt
     6061 acccgattcc acagcatcag tatcgccgaa aaactgcggc tgttcatcct ggaaccgcat
     6121 acgggcaaaa cgcaccaatt gagagtggcg atgaaaagtt tgggcagtcc gattctgggt
     6181 gacagcttat acggcggaac agaatccgaa accatgtttc tgtatgcatg gaaaatacaa
     6241 tttgcctacc aaaaccgaca aatcgaaatt gttgcaccct taaaaaacga atggcagact
     6301 gaaaatatct ctcatgcgct ggaagaattt tatatggcaa aagcaggtta ataaatataa
     6361 attaatttat aaacaatatg ataaatattt ttataaaaat ttttcaagaa aacggttgac
     6421 tgcaaatctc ttcaagtgta tagttcggtt cttcgctgct gcagaagcgg cggacgaact
     6481 gaaaagtata gcacagaatg ttggggatat cgagagatat cttgacaggc ggaagaaata
     6541 ctttataatt caaacgctct ttaacaaaac agattaccga taagtgtgag tgccttgagc
     6601 ctcacactgt ttgaaagaca gacaagataa tgttttgaac attgtcctgt cggtttcttt
     6661 gaagcagacc agaagttaaa aagttagaga ttgaacataa gagtttgatc ctggctcaga
     6721 ttgaacgctg gcggcatgct ttacacatgc aagtcggacg gcagcacaga gaagcttgct
     6781 tctcgggtgg cgagtggcga acgggtgagt aacatatcgg aacgtaccga gtagtggggg
     6841 ataactgatc gaaagatcag ctaataccgc atacgtcttg agagagaaag caggggactt
     6901 tcgggccttg cgctattcga gcggccgata tctgattagc tagttggtgg ggtaaaggcc
     6961 taccaaggcg acgatcagta gcgggtctga gaggatgatc cgccacactg ggactgagac
     7021 acggcccaga ctcctacggg aggcagcagt ggggaatttt ggacaatggg cgcaagcctg
     7081 atccagccat gccgcgtgtc tgaagaaggc cttcgggttg taaaggactt ttgtcaggga
     7141 agaaaaggct gttgctaata tcagcggctg atgacggtac ctgaagaata agcaccggct
     7201 aactacgtgc cagcagccgc ggtaatacgt agggtgcgag cgttaatcgg aattactggg
     7261 cgtaaagcgg gcgcagacgg ttacttaagc aggatgtgaa atccccgggc tcaacccggg
     7321 aactgcgttc tgaactgggt gactcgagtg tgtcagaggg aggtagaatt ccacgtgtag
     7381 cagtgaaatg cgtagagatg tggaggaata ccgatggcga aggcagcctc ctgggacaac
     7441 actgacgttc atgcccgaaa gcgtgggtag caaacaggat tagataccct ggtagtccac
     7501 gccctaaacg atgtcaatta gctgttgggc aacttgattg cttagtagcg tagctaacgc
     7561 gtgaaattga ccgcctgggg agtacggtcg caagattaaa actcaaagga attgacgggg
     7621 acccgcacaa gcggtggatg atgtggatta attcgatgca acgcgaagaa ccttacctgg
     7681 tcttgacatg tacggaatcc tccagagacg gaggagtgcc ttcgggagcc gtaacacagg
     7741 tgctgcatgg ctgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg
     7801 caacccttgt cattagttgc catcattcag ttgggcactc taatgagact gccggtgaca
     7861 agccggagga aggtggggat gacgtcaagt cctcatggcc cttatgacca gggcttcaca
     7921 cgtcatacaa tggtcggtac agagggtagc caagccgcga ggcggagcca atctcacaaa
     7981 accgatcgta gtccggattg cactctgcaa ctcgagtgca tgaagtcgga atcgctagta
     8041 atcgcaggtc agcatactgc ggtgaatacg ttcccgggtc ttgtacacac cgcccgtcac
     8101 accatgggag tgggggatac cagaagtagg tagggtaacc gcaaggagtc cgcttaccac
     8161 ggtatgcttc atgactgggg tgaagtcgta acaaggtagc cgtaggggaa cctgcggctg
     8221 gatcacctcc tttctagaga aagaagaggc tttaggcatt cacacttatc ggtaaactga
     8281 aaagatgcgg aagaagcttg agtgaaggca agattcgctt aagaagagaa tccgggtttg
     8341 tagctcagct ggttagagca cacgcttgat aagcgtgggg tcggaggttc aagtcctccc
     8401 agacccacca agaacggggg catagctcag ttggtagagc acctgctttg caagcagggg
     8461 gtcatcggtt cgatcccgtt tgcctccacc aatactgtac aaatcaaaac ggaagaatgg
     8521 aacagaatcc attcagggcg acatcacact tgaccaagaa caaaatgctg atataataat
     8581 cagctcgttt tgatttgcac agtagatagc aatatcgaac gcatcgatct ttaacaaatt
     8641 ggaaagccga aatcaacaaa caaagacaaa gcgtttgttt tgatttttta ttctttgcaa
     8701 aggataaaaa atcgctcaca agagaaaaga aaacaaacac agtatttggg tgatgattgt
     8761 atcgacttaa tcctgaaaca caaaaggcag gattaagaca caacaaagca gtaagcttta
     8821 tcaaagtagg aaattcaagt ctgatgttct agtcaacgga atgttaggca aagtcaaaga
     8881 agttcttgaa atgatagagt caagtgaata agtgcatcag gcggatgcct tggcgatgat
     8941 aggcgacgaa ggacgtgtaa gcctgcgaaa agcgcggggg agccggcaat aaagcaatga
     9001 ttccgcgatg tccgaatggg gaaacccact gcattctgtg cagtatccta agttgaatac
     9061 atagacttag agaagcgaac ccggagaact gaaccatcta agtacccgga ggaaaagaaa
     9121 tcaaccgaga ttccgcaagt agtggcgagc gaacgcggag gagcctgtac gtaataactg
     9181 tcgagataga agaacaagct gggaagcttg accatagtgg gtgacagtcc cgtattcgaa
     9241 atctcaacag cggtactaag cgtacgaaaa gtagggcggg acacgtgaaa tcctgtctga
     9301 atatgggggg accatcctcc aaggctaaat actcatcatc gaccgatagt gaaccagtac
     9361 cgtgagggaa aggcgaaaag aaccccggga ggggagtgaa acagaacctg aaacctgatg
     9421 catacaaaca gtgggagcgc cctagaggtg tgactgcgta ccttttgtat aatgggtcaa
     9481 cgacttacat tcagtagcga gcttaaccga ataggggagg cgtagggaaa ccgagtctta
     9541 atagggcgat gagttgctgg gtgtagaccc gaaaccgagt gatctatcca tggccaggtt
     9601 gaaggtgccg taacaggtac tggaggaccg aacccacgca tgttgcaaaa tgcggggatg
     9661 agctgtggat aggggtgaaa ggctaaacaa actcggagat agctggttct ccccgaaaac
     9721 tatttaggta gtgcctcgag caagacactg atgggggtaa agcactgtta tggctagggg
     9781 gttattgcaa cttaccaacc catggcaaac tcagaatacc atcaagtggt tcctcgggag
     9841 acagacagcg ggtgctaacg tccgttgtca agagggaaac aacccagacc gccagctaag
     9901 gtccccaatg atagattaag tggtaaacga agtgggaagg cccagacagc caggatgttg
     9961 gcttagaagc agccatcatt taaagaaagc gtaatagctc actggtcgag tcgtcctgcg
    10021 cggaagatgt aacggggctc aaatctataa ccgaagctgc ggatgccggt ttatcggcat
    10081 ggtaggggag cgttctgtag gccgatgaag gtgcattgta aagtgtgctg gaggtatcag
    10141 aagtgcgaat gttgacatga gtagcgataa agcgggtgaa aagcccgctc gccgaaagcc
    10201 caaggtttcc tgcgcaacgt tcatcggcgt agggtgagtc ggcccctaag gcgaggcaga
    10261 aatgcgtagt cgatgggaaa caggttaata ttcctgtact tgattcaaat gcgatgtggg
    10321 gacggagaag gttaggttgg caagctgttg gaatagcttg tttaagccgg taggtggaag
    10381 acttaggcaa atccgggtct tcttaacacc gagaagtgac gacgagtgtc tacggacacg
    10441 aagcaaccga taccacgctt ccaggaaaag ccactaagct tcagtttgaa tcgaaccgta
    10501 ccgcaaaccg acacaggtgg gcaggatgag aattctaagg cgcttgagag aactcaggag
    10561 aaggaactcg gcaaattgat accgtaactt cgggagaagg tatgccctct aaggttaagg
    10621 acttgctccg taagccccgg agggtcgcag agaataggtg gctgcgactg tttattaaaa
    10681 acacagcact ctgctaacac gaaagtggac gtatagggtg tgacgcctgc ccggtgctgg
    10741 aaggttaatt gaagatgtga gagcatcgga tcgaagcccc agtaaacggc ggccgtaact
    10801 ataacggtcc taaggtagcg aaattccttg tcgggtaagt tccgacccgc acgaatggcg
    10861 taacgatggc cacactgtct cctcctgaga ctcagcgaag ttgaagtggt tgtgaagatg
    10921 caatctaccc gctgctagac ggaaagaccc cgtgaacctt tactgtagct ttgcattgga
    10981 ctttgaagtc acttgtgtag gataggtggg aggcttagaa gcagagacgc cagtctctgc
    11041 ggagccgtcc ttgaaatacc accctggtgt ctttgaggtt ctaacccaga cccgtcatcc
    11101 gggtcgggga ccgtgcatgg taggcagttt gactggggcg gtctcctccc aaagcgtaac
    11161 ggaggagttc gaaggttacc taggtccggt cggaaatcgg actgatagtg caatggcaaa
    11221 aggtagctta actgcgagac cgacaagtcg agcaggtgcg aaagcaggac atagtgatcc
    11281 ggtggttctg tatggaaggg ccatcgctca acggataaaa ggtactccgg ggataacagg
    11341 ctgattccgc ccaagagttc atatcgacgg cggagtttgg cacctcgatg tcggctcatc
    11401 acatcctggg gctgtagtcg gtcccaaggg tatggctgtt cgccatttaa agtggtacgt
    11461 gagctgggtt taaaacgtcg tgagacagtt tggtccctat ctgcagtggg cgttggaagt
    11521 ttgacggggg ctgctcctag tacgagagga ccggagtgga cgaacctctg gtgtaccggt
    11581 tgtaacgcca gttgcatagc cgggtagcta agttcggaag agataagcgc tgaaagcatc
    11641 taagcgcgaa actcgcctga agatgagact tcccttgcgg tttaaccgca ctaaagagtc
    11701 gttcgagacc aggacgttga taggtggggt gtggaagcgc ggtaacgcgt gaagctaacc
    11761 catactaatt gctcgtgagg cttgactcta tcatttgaag aacttcaaga gataaaagct
    11821 tactgactga ttcagtcatt accgaatata ttgattaagg ctttaccgat ttgtaacagt
    11881 ttaagtttgg cggccatagc gagttggtcc cacgccttcc catcccgaac aggaccgtga
    11941 aacgactcag cgccgatgat agtgtggttc ttccatgcga aagtaggtca ctgccaaaca
    12001 ccctttcaga aaacccccga ttattcgggg gtttttgctt tacccggaaa aaatgtcggg
    12061 gatggcggga cggcatccgt acggcgtccg gtcgggtttg cggaggaacg gcttgaaact
    12121 ttgggatatt cattttagaa tgactcgttt tatcgtcgca agatgcggtt tattgtttgc
    12181 aacccttaaa ggaaaaacca tgaagaaaat gttcgtgctg ttctgtatgc tgttctcctg
    12241 cgccttctcc cttgcggcgg taaacatcaa tgcggcttcg cagcaggagt tggaggcgct
    12301 gccgggcata ggccctgcga aggcgaaggc cattgcggaa taccgtgcgc aaaacggtgc
    12361 gttcaagtct gtggacgatt tga
//
LOCUS       DAWKST010000055        12249 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.055, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000055 DAWKST010000000
VERSION     DAWKST010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12249
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.055"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            23..259
                     /locus_tag="WJL87_001022"
     CDS             23..259
                     /locus_tag="WJL87_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEZ7799457.1"
                     /translation="MWIFSACSRGADPIGRPFAFVNILENLEIRSTLPLIANRPTFPQ
                     LWVNGELIGGSDIILQMYQSGELKPLIEEHSPKV"
     gene            427..2202
                     /gene="ggt"
                     /locus_tag="WJL87_001023"
     CDS             427..2202
                     /gene="ggt"
                     /locus_tag="WJL87_001023"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ7799458.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSRLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGSYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            2421..2616
                     /locus_tag="WJL87_001024"
                     /pseudo
     CDS             2421..2616
                     /locus_tag="WJL87_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2683..3933
                     /locus_tag="WJL87_001025"
     CDS             2683..3933
                     /locus_tag="WJL87_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ7799459.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLIAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLENVRKQITALCDKYPVYGA"
     gene            4024..4668
                     /locus_tag="WJL87_001026"
     CDS             4024..4668
                     /locus_tag="WJL87_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ7799460.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            4687..6597
                     /locus_tag="WJL87_001027"
     CDS             4687..6597
                     /locus_tag="WJL87_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ7799461.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGVQKLDDGQIIIQNNLKIVYVPQESFFDKDATVFDTVAEGLGEIRDLLRRYH
                     RVGHELENGSGEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKVQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFAYGDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELNPTYGRIRIGSKQEVAYFDQFRS
                     TLNENDTVFYTLGQGNDYVEVGGKKKHIMSYLEDFLFHPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILILDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     IFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            6618..6983
                     /locus_tag="WJL87_001028"
     CDS             6618..6983
                     /locus_tag="WJL87_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799462.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            7094..8569
                     /locus_tag="WJL87_001029"
     CDS             7094..8569
                     /locus_tag="WJL87_001029"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ7799463.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVVDNLSGKIYLIVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSSLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIHAAQMVQEGLDK"
     gene            8566..9111
                     /locus_tag="WJL87_001030"
     CDS             8566..9111
                     /locus_tag="WJL87_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEZ7799464.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKSREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRGAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            9108..10244
                     /locus_tag="WJL87_001031"
     CDS             9108..10244
                     /locus_tag="WJL87_001031"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ7799465.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDA
                     PAAEFADCHLCAPFNDQAALDELAKCAAVTTEFENVNADAMRFLAKHTNVSPGSDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRV
                     KTLDELKAAFAEHGGVDCVLEKMVDLRSEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADVQQQARQMAQRLADELDYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTIDACAADQFQQQVRIMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWLPLQS
                     HPNAHLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            10291..10773
                     /locus_tag="WJL87_001032"
     CDS             10291..10773
                     /locus_tag="WJL87_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ7799466.1"
                     /translation="MSLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            10879..11934
                     /locus_tag="WJL87_001033"
     CDS             10879..11934
                     /locus_tag="WJL87_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7799467.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDSFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            11931..12143
                     /locus_tag="WJL87_001034"
     CDS             11931..12143
                     /locus_tag="WJL87_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799468.1"
                     /translation="MTAMPSEPPFRRHSTHQNRKTAPKIIITRHNGKPDTKPNTAAKM
                     PSEAVGATILIWGALQGRITFLNHPF"
BASE COUNT         3380 a         3338 c         2792 g         2739 t
ORIGIN      
        1 aaggcacgcc ccagtttccc caatgtggat tttcagcgcg tgcagtagag gtgctgaccc
       61 aattggcaga ccctttgctt ttgttaatat cctagaaaac ctagaaattc gcagtactct
      121 accactcatc gccaatcggc caacctttcc acagctttgg gttaatggtg agttaatcgg
      181 tggtagtgat attatcctac aaatgtacca atcaggtgag ctaaaaccac tcattgaaga
      241 gcatagccca aaggtctgat tacttaatat cttaaaataa atatcaaaaa agactgctga
      301 tgacattagc ggtctttttt tgatggtcaa gattgatagt ttggattgaa ttatttagcc
      361 atattaaata caataatcag catgccttgt atgaatcatc aatcaaactc aggcgaagga
      421 gtgcttgtgg ctaaaacata tttattgact gcattgataa tgtctatgac aatctctgga
      481 tgtcaagtca tccatgccaa tcaaggtaag gttaatactc attctgctgt catcacaggt
      541 gcagacgctc acacgcctga acatgcaacg ggactgaccg aacaaaagca ggtgattgca
      601 agtgatttta tggtagcgtc agccaatcca ttagcaacac aagctggcta tgatatctta
      661 aagcaaggcg gtagcgctgc agatgcgatg gtggcggtgc agacgacact aagcttggta
      721 gagccacagt cgtcaggctt gggcggtggt gcatttgtgt tgtattggga taataccgcc
      781 aaaacattga ccacatttga tgggcgtgag acggcaccga tgcgtgcgac gccggaatta
      841 tttttggata aagatggtca accattgaaa tttatggaag cggtggtcgg tggtcgctcg
      901 gtgggtacgc ctgctatccc taaactgatg gagacgatac atcagcgata cggtgtattg
      961 ccttggggaa aattatttga tacgccgatc cgcttggcaa aacaaggctt tgaggtgtcg
     1021 ccaaggcttg ccatctcggt tgagcaaaat cagcagcatt tggcacgcta tccaaaaaca
     1081 gccgcttatt ttttgccgaa tggtgtgccg ctacaagcag gcagcttgct gaaaaattta
     1141 gaatttgctg acagtgttca ggcgttagca gctcaaggtg caaaagctct gcatactggt
     1201 aaatatgccc aaaatatcgt ttcagttgtc caaaatgcta aggataaccc cggtcaatta
     1261 tccttacaag atttatctga ttatcaagtg gtggaacgcc cgcctgtttg tgtgacttat
     1321 cgtatttatg aagtatgcgg tatgggtgca ccaagctcag gtgggattgc tgtgggtcag
     1381 attttgggga ttttaaatga attttcaccc aatcaggtgg ggtatgatgc tgaaggttta
     1441 cgtctcttgg gcgacgcttc taggcttgcg tttgcggatc gtgatgtata tttgggcgac
     1501 cctgattttg taccagtacc cattcgccag ttgatttcta aagactacct aaaacatcgc
     1561 agccggctgc ttgagcagtc ggataaggca ttgcctagcg tatctgcagg cgattttatt
     1621 catgagtggg tgtcctcaca agcgattgag ttaccttcta ccagtcatat atcaattgtg
     1681 gataaggcgg gcaatgtgct atcaatgacg acttccattg aaaatgcgtt tggctcaact
     1741 ttgatggcaa atggctacct gctgaataat gagctgacag actttagttt tgagccaata
     1801 aagcagggca aacaggtcgc caatcgtgtc gagcccggca agagaccacg ctcctcaatg
     1861 gcaccaacca tcgtatttaa agcaggcaag ccttatatgg caatcggctc cccaggtggt
     1921 agccgtatca ttggctatgt cgctaagacg attgtggcac atagtgactg gaatatggat
     1981 atccaagatg ccatcagtgc acccaattta ttaaatcgct ttggtagcta tgaactagaa
     2041 acgggtacaa ccgccctcca gtggcaacaa gcattaaatg atttgggcta taagactgac
     2101 gtgcgtgagc taaattcagg tgttcaagcc attattatcg agccaagcag gctggttggt
     2161 ggtgccgatc cacgccgaga aggtcgagtg atgggtgatt agaggtgaag cttgtatttt
     2221 gtgaatttga ccgcattaag ttaatattaa aataggggga aattgtgagt aatcaaacaa
     2281 aaatacaagc catcaagcaa gcttctgagc agatcttcgc aatttgtgag acgccaaaca
     2341 cagcattaca agccattcat ctgattttaa gacatggtgg ggcaggagag ttgtcttggc
     2401 aagtggtgta tcagcgggtt atggctgata aagatgtggt tggtgctggt tatttgattg
     2461 attttgcaca aactgccgaa aatttaccat ttgatgcatt gcctttgggt agccttgtac
     2521 taacataaag gtaatgagac gctaaaaacc agcatgttaa ataaattacc tgataatgcc
     2581 aaagaaaatt tacgcattat ggactatctg ccttaggttt tgaagtattt ttttatacaa
     2641 aaaacactca tgataccctg attacacaaa ggagccgaga acatgttttc aaaaagcgtt
     2701 accctcgcac aatatgaccc cgatttggca gcagccattg cccaagaaga ccaacgccag
     2761 caagaccacg tcgagctgat tgcctctgaa aactacgtca gctgcgccgt aatggaagcg
     2821 caaggttccc agctgaccaa caaatacgcc gaaggctacc ccggcaaacg ctactacggc
     2881 ggctgcgaat acgtcgatat tgtcgaacaa ttggcaatcg accgcgtcaa aaaactgttc
     2941 ggcgcgcaat acgccaacgt tcaaccgcac tccggctcgc aagccaacca agccgtgtac
     3001 gcttccgttt taaaaccggg cgacaccatt ttgggtatgt ctttggcgca cggcggccac
     3061 ctgacccacg gcgcgagcgt taatatttcc ggcaaactct acaatgccgt tacttatggt
     3121 ttggatgaaa acgaagtgct cgattatgcc gaagtggaac gcttggctct cgaacacaaa
     3181 cccaaaatga ttgtggcggg tgcgtctgcc tacgcgttgc aaatcgactg ggcaaaattc
     3241 cgcgaaatcg ccgataaagt cggcgcatac ctctttgtcg acatggcgca ctacgccgga
     3301 ctgattgccg gtggcgaata tcccaacccc gtgccattct gcgacttcgt gaccaccacc
     3361 acccacaaaa ccctgcgcgg ccctcgcggc ggcgtgattc tgtgccgcga caatacccac
     3421 gaaaaagcac tgaactcttc catcttccca agcctgcaag gcggtccgct gatgcacgtc
     3481 atcgccgcca aagccgtagc gtttaaagaa gcgttgcaac ccgagttcaa acaatacgca
     3541 aaacaagtga aaatcaatgc tgccgccatg gcggaagaac tggtcaaacg cggtttgcgc
     3601 atcgtttccg gccgcaccga aagccacgtt ttcctcgtcg acctgcaacc gatgaaaatc
     3661 accggcaaag ccgccgaagc cgctttgggc aaagcgcaca tcaccgtcaa caaaaacgcc
     3721 attccgaacg atccggaaaa acccttcgtt acctccggca tccgcatcgg ctccgccgcc
     3781 atgaccacac gcggttttaa cgaagccgac gcacgcgtat tggcgaactt ggttgccgac
     3841 gtattgtcta accccgaaga cgaagcgaat ctggaaaacg tccgcaaaca gattaccgcc
     3901 ctctgcgaca aataccccgt ttacggcgcg taaacttttt taaaaacaaa tgccgtctga
     3961 aaatttcaga cggcattttt ccaatcggtt aaaatacgcg gtttgttttt ttaggaacgc
     4021 gccgtgcttg cctccgccat cgacttcatc ctccatatcg accaacacct gctcgcgctg
     4081 tcggcgcaat acggtgtgtg gatttatgcg attctgtttt tgattgtttt ttgcgaaacc
     4141 ggcctgattg ttacgccctt actgcccggc gattcgctgc tgtttgccgc cggcgggatt
     4201 gccgcgctgg gcggtatgga tattcatttg atggttgcat tattaagcct tgccgccata
     4261 ctcggcgacg cgctcaattt taccgtcggc aaatatttcg gcggcaggct gttcgccaac
     4321 cccgattcta aaatcttccg acgtgaatat ctcgataaaa cccaccgttt ttacgaaaaa
     4381 cacggcggca aaaccatcat cattgcccgt tttatgccca tcgtccgcac ctttgccccc
     4441 ttcgtcgccg gtatggggaa aatgcactat gccaaattta tccgctacaa catcatcggt
     4501 ggcttgttgt gggttatcct gttttcttac gcgggctatt tctttgccaa cttccccgtc
     4561 gtaaaaaaca atctgggctt ggtgatgggc ggcatcatta ttgtttccgt cctgccgggt
     4621 gcggttgaaa ttgcccgcgc caaacttgcg gtaaaatcca aacggtaact gttgagaatt
     4681 tgcatcatga atatattatc tgtagaaaac gcttcttttg ccatcggcca cgttgccttg
     4741 ctcgacaaaa cttcttttca actcgacagc ggcgaaaaag tcggcttgat cggacgcaac
     4801 ggcgcaggta agtcttcgtt tctgaaaatc ctcgccggcg tgcaaaagct cgacgacggg
     4861 cagattatca ttcaaaacaa cctcaaaatc gtttatgtac cgcaggaatc cttttttgat
     4921 aaggacgcaa ccgtatttga taccgttgcc gaaggtttgg gtgaaattcg cgatttattg
     4981 cgccgttacc accgcgtcgg ccatgagttg gaaaacggtt cgggtgaggc tttgttgaaa
     5041 gaactcaacg aattacaact tgaaatcgaa gcgaaggacg gctggaagct ggatgcggca
     5101 gtcaagcaga ctttggggga actcggtttg ccggaaaacg aaaaaatcgg caacctttcc
     5161 ggcggtcaga aaaagcgtgt cgccttggcg caggcttggg tgcagaagcc cgacgtattg
     5221 ctgctggacg aaccgaccaa ccatttggat atcgacgcga ttatttggct ggaaaatctg
     5281 ctcaaagcgt ttgaaggcag cttggttgtg attacccacg accgccgttt tttggacaat
     5341 atcgccacgc ggattgtcga actcgatcgc ggtattttgc gttcctatcc cggctcgttc
     5401 tccaaataca gcgaaaaaaa agtgcaggaa ttggcggttg aagcggaaca caaccgcctg
     5461 tttgacaaat tccacgcgca ggaagaagcg tggatacgca aaggcatcga agcgcgccgc
     5521 acccgcaacg aaggccgcgt gcgccgtttg gaagagctgc gccgccaacg cgccgaacgc
     5581 cgcaatgtgc agggacaggt caacttcaag ctcgacagcg gcgagaaaag cggcaaaatc
     5641 atcgccgaat tggaacatgc ctcgtttgcc tatggcgaca aagtcattat ggacaaattc
     5701 tccgcgattt tgcagcgcgg cgataaaatc ggtctaatcg gccccaacgg catcggcaaa
     5761 accacatttt taaaactgat tttaggcgaa ctcaacccga cttacggcag aatccgtatc
     5821 ggcagtaagc aggaagttgc ctatttcgac caattccgca gcacgttgaa cgaaaacgac
     5881 accgtgtttt acacgctcgg tcaaggcaac gattacgtcg aagtcggcgg caagaaaaaa
     5941 cacataatga gctatctgga agatttctta ttccatccag cccgcgcaca aagtcccgtt
     6001 tcatcgctct ccggcggcga acgcaaccgc cttttgctgg caaaactctt tacccgtcct
     6061 gctaatattt tgattttgga cgaaccgacc aacgacttgg atatcgatac ccaagagctg
     6121 cttgaagatt tgctgcgcga ttatcaaggc acggtattcc ttgtctcaca cgaccgtatg
     6181 ttcctcgata acgtgattac ccaaagcatt atttttgaag gaaatggaag gttaaaagaa
     6241 tatatcggcg gctatcagga ctacatcgac gcaaaatcac gcgaagcgaa gattcagacg
     6301 gaatccgcac ccaaaacagc cgatgccgaa ccggtgaaag aaaaacccaa agccaaccgc
     6361 acggtcaaac tgtcctacaa agaacagcgc gaactcgacg ccctgcccga cgaaatcgcc
     6421 gccttggaag ccgaacaggc agaaatcaac gcgcagcttt ccgatcccga aatcttcaaa
     6481 gattacgaaa aagccggcgc actgcaaagc cgcgccgaag aaatcgaaat gctgcttttg
     6541 gaaaagctgg aacgctggga gctgctggaa accaaacaga acggcaatgc cgtctgaaat
     6601 caaacaggaa gaaaaaaatg ttcaaacgac ctgaagaaat cattgtcctg atacttgccg
     6661 tcctctggat tgccggtacg tattttctcg ccgccctgtt tggagcggat gcttataccg
     6721 tgttaaaaat cactgccctt accctgcttt ggtctgccgc atcattcctc ctttggcaaa
     6781 aaaacctcag ccggcatatc tggccgctgc tgctcggctg cctgactgcc tgttggtggc
     6841 cgtatctgaa tcaatcggtc gcacccggtt ctttaccttg gtatgcatca tggacgttca
     6901 aaaccatctt agccctgatt cccgtaaccg caggttatct gtttaaatgg aaacaatacc
     6961 ggcagcgcaa aacaaagccg taagcccgtc cttttcagac ggcatctccg gtcagactgt
     7021 caacaattcc gataaaatgc tatactcgcg cccgttgccg cgccgtccga aaccgccctt
     7081 tgggaaagca gaaatgatca gcaaacaaga ataccaagcc caagccgctc aaggctacaa
     7141 ccgcatcccg ctcgtgcaag aactccttgc cgatttggat acgccgcttt ccctctatct
     7201 caaactcgcc aaccgcccct atacctacct gctcgaatcc gttgtcggtg gcgaacgttt
     7261 cggccgctat tcttttatcg gcctgccttg cagccactat ctcaaagcca gcggcaaaca
     7321 cgtcgatgtt tatcaaaacg gcgaaattgt cgagcaacac gacggcaacc ccctgccctt
     7381 tatcgaagcc ttccacaacc gcttcaaaac acccgaaatc ccaagcctgc cgcgctttac
     7441 cggcggactg gtcggctact tcggttacga aaccatctac aattttgaac acttcgccca
     7501 ccgcctgaaa aacaccacca aagccgaccc gctcggcacg cccgacatcc tcctgatgct
     7561 ctcgcaagag cttgcagtag tggataacct aagcggtaaa atctacctga ttgtttacgc
     7621 cgatccgtcg cagcccgacg gctacgaacg cgcacgcgaa cgcctcgaag acatccgtac
     7681 acagttacgc caaagttgcg ccatcccgct ttcgctcggc agcaaacaca ccgaagccgt
     7741 cagcgaattc ggcgaagagc cgttcaaagc ctgcgttaac aaaatcaaag actacatctt
     7801 tgcaggcgac tgcatgcagg ttgtccccag ccagcgcatg agcatggaat tcaccgacag
     7861 ctcgctcgcc ctctaccgcg ccttgcgcac gctcaaccct tcgccttatc tcttctacta
     7921 cgatttcggc gatttccaca tcgtcggctc ctcgcccgaa atcctcgtcc gccgcgaacg
     7981 cgacgatgtc atcgtccgcc ccatcgccgg cacgcgcctg cgcggcaaaa cccccgccga
     8041 agaccttgcc aacgaacaag atttgttaag cgatgccaaa gaaatcgccg aacacgtcat
     8101 gttgattgac ttaggacgca acgacgtcgg ccgcatcagt aaaaccggcg aagtcaaagt
     8161 taccgacaaa atggtgattg aaaaatactc ccatgtaatg cacatcgttt ccaacgtcga
     8221 aggccgtctg aaagacggca tgaccaacat ggacatcctc gccgctacct tccctgccgg
     8281 cacactctcc ggcgcaccca aagtccgcgc catggaaatc atcgaagaag tcgaaccgag
     8341 caaacgcggc atctacggcg gtgccgtcgg cgtatggggt ttcaacaacg acatggattt
     8401 ggcaatcgcc atccgcaccg ccgtagtaaa aaacaacacg ctatacgtcc aaagcggcgc
     8461 gggcgtggtt gccgactccg atccggcctc cgaatggcag gaaacacaaa acaaagcccg
     8521 agccgtcata cacgccgcgc agatggtgca ggaagggttg gataaatgac ttatgaaaaa
     8581 ctttatgagc ttcgccaaac actcagaccg actaccaaag acggtctcta tacagataat
     8641 gaaaaaagtc gagaaatcct aacggttaga tggagcggca acacagaaaa tcctaaaaat
     8701 ttctcagctg ttgctatagg aattaatcca agtaaagcaa atgatgaaag aagcgacaaa
     8761 acccttaccc aacttgcacg tttcttggat atgtacggtt ttaccaactt caaaatgtta
     8821 aatatttttt ccagttactc tacacagcaa acaggaatta gggcaaacac ccaaaccgat
     8881 ttttccaaat ttaaaggatg tttggaagat gctgatatga ttattcttgc atggggtaca
     8941 gaccgcggtg cttataagga tgaaaaaaac agaatactgg aatttctaaa agcagaaaaa
     9001 tttatggaaa aagtattctg tatttctgaa actggtaatt ccagcgatac aagacatccg
     9061 tcaagaatta gttatagcta tcaacttgta caatttgaag agtcagcatg aaaaacatat
     9121 ctctttctcc gcccgccatg cttggcatcc tcggcggcgg acaattaggc agaatgttta
     9181 ccgttgccgc caaaaccatg ggctacaaag taaccgttct cgaccccgat ccggacgcgc
     9241 cggcagcaga atttgccgac tgtcatttat gcgcgccgtt taacgaccaa gctgctttgg
     9301 acgaattggc aaaatgcgcg gcggtgacca ctgaatttga aaacgtcaat gccgatgcga
     9361 tgcgttttct cgccaaacac accaatgttt cccccggcag cgactgcgtt gccatcgcgc
     9421 aaaaccgcat tcaggaaaag gcatggatac gcaaagcagg attgcaaacc gcgccgtatc
     9481 aagcaatttg caaagccgaa gacatcactg aagaaagcat acaatttctg cccggcatcc
     9541 tgaaaacggc tacgttgggc tacgacggca aaggtcaaat ccgcgtaaaa acattggatg
     9601 aactcaaagc cgcgtttgcc gaacacggcg gcgtggattg cgttttggaa aaaatggtgg
     9661 atttgcgcag tgaaatttcc gtaatcgtat gccgtttgaa caatgacaac gtgcaaactt
     9721 tcgaccctgc cgaaaacatc cacgaaaacg gcatcttggc ttattccatc gtccccgcgc
     9781 gactgagtgc cgacgtgcag caacaggcgc ggcagatggc gcaacgcttg gcggacgaat
     9841 tggattatgt cggcgtattg gcggtagaaa tgtttgttgt cggtgacacg catgaattgg
     9901 tcgtcaacga aatcgccccg cgcccgcaca attccggaca ccatacgata gatgcctgcg
     9961 cagcagacca gttccagcag caggtacgca ttatgtgcaa cctgccgcct gccgatacca
    10021 aattactgag ttcttgctgt atggcaaata ttttgggcga cgtttggcag gaagacggcg
    10081 gcgaaccgga ttggctgccc ttgcaaagcc atccgaatgc acacctgcac ctttacggca
    10141 aaaaaaccgc gcacaaaggg cggaaaatgg gacactttac cgttttaacc accgattcgg
    10201 acaccgcatt tcaagaagca aaaaaactgc atcagtccct ataatccgac caacggcaca
    10261 gatgccgtct gaagccgccg acataccgcc atgagcctgc tgacactgtt acgccccgcc
    10321 accgtgcaag actgcaaaga catcttcaaa gtccacctgc actccgtcca atacacctgc
    10381 atcctcagct acaacgaaca cgccctaaaa gtatgggaag gcttgctaaa cacagaaagc
    10441 tacctgccga ccatttccga tcccgacaaa gcactttggg ttgccgaata caaaggcaac
    10501 atacagggat ttttccaaat cgactgccaa gaagcacaac tcgatgcact ttacgtccac
    10561 cccctgttcc acaacctcgg actgggcact gccctacttc atcaggcgga aaccatcgcc
    10621 cacaaatccg gtttgagctt tctgaaactc tacgcatcgc tcaactccgt cccgttttac
    10681 cttctcaacc gctacgaatc gctcggttcg gcagtattgc agcttgaccc cagcatcaaa
    10741 atcaaatgcg aactgatgcg gaaacacctc tgatccgtcc ttataaccaa aactgataat
    10801 cttcaaatca aacattattt ccacaagcgc agacagccgt ataaagttgc cccatctacc
    10861 aaccgacagg acaagcatat gaaaacctac gcaccggcac tctataccgc agccctgctc
    10921 accgcctgca gccccgcagc cgattcaaat catccgtccg gacaaaatgc cccggccaat
    10981 accgaatccg acggaaaaaa cattaccctg ctcaacgcct catacgatgt ggcacgggat
    11041 ttttacaaag aatacaaccc cttatttatc aaaacatacc aatccgaaca ccccggcaca
    11101 tccgtcagca tccaacagtc ccacggcggc tccagcaaac aggcattatc cgtagccaac
    11161 ggccttcaag ccgatgtcgt aaccatgaac caatcctccg acatcgacct gctcgaaaaa
    11221 aaaggactgg tagaaaaagg ctggcaacaa gccctccccg accacgccgc gccctacacc
    11281 agcactatgg ttttccttgt ccgaaaaaac aaccccaaac agatccgcga ttggaacgac
    11341 cttgccaaag acggcgttaa catcgtcatc gccaatccca aaacctcggg caacggacgc
    11401 tacgccttcc tcggcgcata cggttacggt ctgaaaacca ccaacggcaa cgaacaggaa
    11461 gcccaaaaac tcgtcgcatc catcctcaaa aacacccccg tttttgaaaa cggcggacgc
    11521 gccgccacca ccaccttcac acaacgcaac atcggcgacg tactcattac ttttgaaaac
    11581 gaagccaact acgtcagcaa aaaactgacc caaggacagt ttgaaatcgt ctatccgagc
    11641 tacaccattt ccgccgaaag ccccgtcgcc gtcgtcaaca gcgtcgtcgc gaaaaaaggc
    11701 acgcaaaaaa ccgcccgcgc ctatctcgaa tacctttgga gcgaaccggc gcaagaactc
    11761 gccgcctcac tctacctgcg cccccgcaac cccgaagtat tggcaagaca caaagccgac
    11821 ttcccagact tagactcatt ctctcccgaa gaaaaattcg gcggatggga caacatcatg
    11881 aaaacctact tcgccgacgg cggcatattc gaccgcctga ccgcacaaaa atgaccgcta
    11941 tgccgtctga accgcctttc agacggcatt ccacacatca aaacagaaaa accgcgccca
    12001 aaatcatcat cacaagacac aacggcaaac cggatacaaa acccaacacc gccgccaaaa
    12061 tgccgtctga agccgtcggc gcaaccatcc ttatctgggg cgcgcttcag gggcgcataa
    12121 ctttccttaa ccatcccttt tgacagccaa ataaacagtg cttttgcctg tcagaaacaa
    12181 aaccaatatt ttgcagttcg tatcggaaca tttatagtgg attaacaaaa accagtacag
    12241 cgttctcgc
//
LOCUS       DAWKST010000056        12249 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.056, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000056 DAWKST010000000
VERSION     DAWKST010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12249
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.056"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(592..1527)
                     /locus_tag="WJL87_001035"
     CDS             complement(592..1527)
                     /locus_tag="WJL87_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEZ7799469.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCPESIDYVDERKGVP
                     LAAMKYILMYTEAAASHAYLFEHDLKKFKQYAYVAGKLGILQSVDDEDPEPFFFPCDM
                     LNIQDPMFLMLMSDSPQLREFLVRNIDNIANDTEAFVNRYDLNRHMIYNTLLMVEGKQ
                     LDRLKQRSEKVLAHPTPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMA
                     AKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDQEIAPRDLIVYDPLPADEYQDIFDF
                     MKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
     gene            complement(1530..2150)
                     /locus_tag="WJL87_001036"
     CDS             complement(1530..2150)
                     /locus_tag="WJL87_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799470.1"
                     /translation="MLGVVPGIGESIQAYKVAKAAKNLQGMKKALDKAATVATAQGYV
                     SKTKIKIGQIELRVTAATDKQLLKAIGEGRDTTGKMTEQLFDSLAKQNGFRVLSGGKY
                     GGNNGFDHVWQAADGSVVLIVESKQIRNGTVQLNPNGAGGYTQMSREWIKQVVKSLPD
                     GSPAKAVVLKANQNGKLKTVIAGVDRQTGKAVILSVKVPSKTNIRR"
     gene            complement(2229..2654)
                     /locus_tag="WJL87_001037"
                     /pseudo
     CDS             complement(2229..2654)
                     /locus_tag="WJL87_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            complement(2702..2974)
                     /locus_tag="WJL87_001038"
     CDS             complement(2702..2974)
                     /locus_tag="WJL87_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799471.1"
                     /translation="MNKRMKMCPACQQGYLYHSKPKYLHDEIILCDECDAVWLKGMNI
                     FYGEYEKDFYSYVPFMESQGITSECIWEGDLFDHPYYEDENSNDMD"
     gene            complement(3162..3650)
                     /locus_tag="WJL87_001039"
     CDS             complement(3162..3650)
                     /locus_tag="WJL87_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799472.1"
                     /translation="MKKDIFYCEQWSYGYKRLHKPFSEKQAEEKHIKGELYSAVIGSA
                     TQPEYVITLREEVGFFSVDFFDKFGRDYLTHQFQKYSNSNYYFLSMAVWRDYITLDSH
                     DLAEGYTYFFNENTDDCYVLKEDFINNERYEKTELYSQKDKVILFPKFGEYDLVLNPD
                     II"
     gene            complement(3647..6148)
                     /locus_tag="WJL87_001040"
     CDS             complement(3647..6148)
                     /locus_tag="WJL87_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ7799473.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRIILSAADIAAENGK
                     IKLQSYGDQSYYAGQGELYTLERRSYKTGKWYNRKHITEVKEHKNAKPDAVNLSASQG
                     IDIKSGGSIDAYATVFDAPKGSINIEAGRKLTLYAVEELNYDKLDSHKKRKFLGITYD
                     KVHDTSTQQMQTALPSRVVAESANLQSGWDTKLQGTQFETTLGGAVIRAGVGDQARAD
                     AKIILEGIKSSIHTETVSSSKSALWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYI
                     VDIPKGNLKTQIETLTKQPEYAYLKQLQVAKNINWNQVQLAYDKWDYKQEGMTPAAAA
                     VVVIVVTVLTYGALSAPAAAGTAGAAGAGAGGAAAGTAAGTGVAAGTAATTGVAAGTS
                     AAAITTAAGKAALASLASQAAVSLINNKGDINHTLKELGKSSTVRQAATAAVTAGVLQ
                     GISGLNTQAAEAVSKHFHSPAAGKLTANLINSTAAASVHTAINGGSLKDNLGDAALGA
                     IVSTVHGEVASKIKFNLSEDYIAHKIAHAIAGCAAAVANKGKCRDGAIGAAVGEMVGE
                     TLLDGRDVGKLSPQERQKVIAYSQIIAGSAVALVKGDVNTAANAATVAVENNSLLARR
                     RVNIRWTPRQELEHEYAILEIQAITNQIRRLDPKFNGIAIMRNPREPWTRHDVQTYRQ
                     YYNQLRESRGIPVLPIYRMRINNGNEFNRIMSSKYPYNELYVANPKSVTGHFRVDSYN
                     PATREIISRKFTQFSQIQESTGIGYIKEAVRKYSPGAVISNVPSTPASLRGRKLTGKL
                     ILEVPAQVNPIPQSVLRAAQEENVIIRDTTGRIYK"
     gene            complement(6398..6781)
                     /locus_tag="WJL87_001041"
     CDS             complement(6398..6781)
                     /locus_tag="WJL87_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799474.1"
                     /translation="MEKIKNYIEFKKLFKNITEKFVRDLDITLIEAAEYSIIEFEIYL
                     SEYPEDSTFIFTAWAIYLLENNCLNERGKDNFFIAEMKKCYQQTNLDRIKSIYSSSDY
                     HIFAKDVNKIQQTFKLNLANIMQQE"
     gene            complement(6786..9173)
                     /locus_tag="WJL87_001042"
                     /pseudo
     CDS             complement(6786..9173)
                     /locus_tag="WJL87_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
     gene            complement(9280..9543)
                     /locus_tag="WJL87_001043"
     CDS             complement(9280..9543)
                     /locus_tag="WJL87_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799475.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            complement(9562..10992)
                     /locus_tag="WJL87_001044"
                     /pseudo
     CDS             complement(9562..10992)
                     /locus_tag="WJL87_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224022.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
     gene            complement(11377..11751)
                     /locus_tag="WJL87_001045"
     CDS             complement(11377..11751)
                     /locus_tag="WJL87_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ7799476.1"
                     /translation="MDEKQKIKILDFQIDLSSIFNSYKNQMGINIQDKNLKNNFCSFM
                     EELLNDGSIRLHDYTDGIGIPLTGTSKEQVQKLKDIWPTLEDAQAIWPEDPWYYLEWL
                     WWDIACPIDLADLPNIDIYEQA"
     gene            complement(11770..>12249)
                     /locus_tag="WJL87_001046"
     CDS             complement(11770..>12249)
                     /locus_tag="WJL87_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799477.1"
                     /translation="AAPKETINNLANLAKAEQQILFRIAQRDTQLDAWKTGFNNRVRK
                     GAGLLDASNIPITINGKTIKPVQAISLKGAPVYSGVSEQEIFALYRQMTGQNPNFRVL
                     PDGRLANGIISTGEWAGTKIALRNFSKTENSTQARWTLDLQNPPSFIKGTKLELKFQ"
BASE COUNT         2964 a         2831 c         2591 g         3863 t
ORIGIN      
        1 cttcctgttg tatctctaat gataacattt tcttcttgtg ccgcccttaa tacagattgt
       61 ggaattggat tgacctgagc aggaacttct aaaataagtt ttccttcaag ctgtcttcct
      121 cttagcagat taggtgtact tggaacattg gaaatgacaa agtgagtgcg caaagccacc
      181 tgaaggcgat gtgtttgaac ctgttgaaag cggccaacag gctaaatgcg cccgctgccg
      241 cctaaaaggc agcccggatg cctgattatc gggtgtccga ggaggattaa gggggtattt
      301 gggtaaaatt aggaggtatt tggtacgaaa acagccgaaa tcctgtgttt gggtttcgga
      361 tgtcggggaa gggctttttt gcaaaggtct caactcatgt tattgcaaag atctcatgcc
      421 ttgttttgcc agcaggttga gttcttctcc ggcaatcagc gtggtaaagc cccgccacaa
      481 cacagatcta tgtactgtgg aagggcttca atttatagct ttcaaaaatc tttcagcttt
      541 aagacggcct aaagttgtaa tctataaaac aggagctcag agttttacgg ctcactctcg
      601 cttcgcgtta ccaaatacca gcttatcggt tttgaacgta tagtaatcta tccaatcctg
      661 cagatattca tacgggtaag acaagtcata ctgtttcata aaatcgaaga tgtcttgata
      721 ttcgtctgcc ggcagcggat cgtaaacaat caaatccctc ggtgcgattt cttgatctat
      781 gcccaaatcg aaaccgtgca tggatgcgat tttggcgtag gtaacgattt gcggctgcag
      841 gtagaaatcg aaataggaca atgtttcttt ggcagccata cgcgcggttt ttttatcgaa
      901 aagcggctct aaggcggcct tcatcgcctc ggcatcctgt tcggcgaaag cgaggaagaa
      961 gcggtaatcg tacaaccgct tttgcagcca tttgctaggg gtgggatgcg ccaagacttt
     1021 ctcgctacgt tgtttcaacc gatcaagctg cttaccctcc accatcagca gagtattgta
     1081 aatcatatga cggttgaggt cgtatcggtt tacgaaggct tctgtatcgt tggcgatgtt
     1141 gtcgatattg cgcaccaaaa actcgcgcag ctgcgggctg tcgctcatca gcatcagaaa
     1201 catcggatct tgaatgttga gcatgtcgca gggaaagaag aagggttcgg ggtcttcatc
     1261 atctacactc tgcaaaatac ccaactttcc tgcaacataa gcatattgct tgaatttctt
     1321 aagatcatgt tcaaatagat aggcatggga agccgcagct tcagtgtaca ttaaaatgta
     1381 tttcatcgct gcaagcggaa cgccttttct ttcatctaca taatctatag attcggggca
     1441 accgctattg aaattagcag tattgcctat gattacatta gtaatatgtt cataccattt
     1501 ttgggtggtc atcatattgt gccccattgt tatctcctta tattggtttt agaaggaact
     1561 ttgacagaaa gaataacggc cttacctgtt tgacgatcaa cgcctgctat taccgttttt
     1621 aatttgccgt tctgatttgc ttttaagaca actgccttag caggactacc atcaggtaaa
     1681 ctttttacaa cttgtttaat ccattcacga ctcatctgcg tatatccacc cgcaccattc
     1741 ggattcagct gtaccgtacc gttcctaatc tgcttacttt ctacaatcaa aacaacacta
     1801 ccatcggcag cctgccatac atgatcaaaa ccgttatttc cgccgtattt gccgcccgaa
     1861 agcactctga agccattttg tttagctaaa gagtcaaata actgctcggt cattttacct
     1921 gtcgtgtccc ttccttcgcc aatagctttc agcaattgtt tgtcagttgc tgcagtaacc
     1981 cttaattcaa tttgaccgat tttgattttg gttttactga catagccctg tgcagtggca
     2041 acggttgctg ccttgtccaa ggcttttttc atgccttgta aattttttgc cgctttcgct
     2101 actttatagg cctgtatcga ttcaccgata cccggaacca cacccagcaa agcaaacagg
     2161 tgatcggcag cggtctgtgc ttgtacaaaa ctctgtatat caccgataat tggaataaat
     2221 cctgtctgaa gaagcaaaac ttctcgatat tcttcaggat tttcacgtat ccatttttgt
     2281 tgttcctttt gaaattcatc acactttgca ccaacgcatc taggatacaa agtattattc
     2341 tccaccgcta tctgtgccgc attcgccgct gtattcacat ccccgcctac aacggcagac
     2401 gcagtgccgg caaccagttt tgaataggca gtaatcttcg ctttagattt tttgatttcg
     2461 gtcgcactca tacgactgaa atcagtattc ttaaccaaag cctcaccaac aatctcaccg
     2521 actgcagcgc ctatcgcgcc atcctgacac ttgcccttat tcgccgccgc tgccgcacag
     2581 cccgctatgg catgggcaat cttgtgggtg gtatatcgct gccagccctc tctgttgata
     2641 atcttgcgta tgattacgcc gtttgatggc taatcgtacc tacgcaggct taaaatttcc
     2701 atcaatccat atcatttgag ttttcatctt cataatatgg atgatcaaac aaatctcctt
     2761 cccaaataca ttcactcgtt ataccttggg attccatgaa aggaacataa gaataaaaat
     2821 ctttttcata ttctccataa aatatattca tacctttgag ccatactgca tcgcattcat
     2881 cacacagaat aatttcatca tgaagatatt taggtttcga atggtagaga tagccttgtt
     2941 gacaagcagg acacattttc attctcttat tcatgattac tccatataat cgatttattt
     3001 ttgtgggtaa gcagtagtga tttgattgct gcctttagtc tgtacaacaa tacgtatata
     3061 gcaatctgat aatttcctgg tttttatcag ctttatcctc agattccaaa cccaataata
     3121 aaagccgttt gaaatttttt tcagacggcc ttaaaactta attaaataat gtccggattt
     3181 aacaccaaat catattcgcc aaactttgga aatagaatta ccttatcttt ttgggaatat
     3241 aattctgttt tttcatatcg ctcattatta ataaaatcct ctttcaaaac atagcaatca
     3301 tccgtatttt cattgaagaa ataagtatat ccttctgcta agtcatgaga gtctaaagtt
     3361 atataatctc tccatacagc catagaaaga aaataataat tcgaattgga atatttttga
     3421 aattgatggg ttaaataatc ccttccaaat ttatcgaaaa aatctaccga aaaaaaacct
     3481 acttcctctc gcaaggtaat tacatattca ggttgtgtcg ccgaacctat tacggcacta
     3541 tataattccc ctttaatatg tttttcttca gcttgtttct cagaaaaagg cttatgaagt
     3601 ctcttataac cataagacca ctgctcacaa taaaaaatat ctttcttcat ttgtaaatcc
     3661 ttcctgttgt atctctaatg ataacatttt cttcttgtgc cgcccttaat acagattgtg
     3721 gaattggatt gacctgagca ggaacttcta aaataagttt tcctgtaagt tttcttcctc
     3781 ttagcgaagc aggtgtactt ggaacattgg aaatgacagc accagggcta tattttctaa
     3841 cagcctcctt gatataacca atccccgtac tttcttggat ttgagaaaat tgggtaaatt
     3901 ttcttgaaat aatttccctt gttgcaggat tatacgaatc taccctaaaa tgtcccgtta
     3961 ccgatttagg attggctaca taaagctcat tataagggta ttttgatgac atgatacggt
     4021 taaattcatt gccgttgttt atcctcattc tataaattgg gagaacagga atgcctctgg
     4081 attcccttaa ttgattataa tattgcctgt atgtttgtac atcatgtctt gtccacggct
     4141 ctctaggatt cctcataata gcaatcccgt taaatttcgg atccagcctt cggatttgat
     4201 tggtaatggc ctggatttca agaatggcat attcatgttc caattcttgt cgcggagtcc
     4261 aacgtatatt taccctcctg cgagctaaaa gactattatt ctccactgcc acagtagccg
     4321 cattcgccgc cgtattcaca tcccctttaa ccaatgccac tgcgctgcct gcgataatct
     4381 gcgagtaggc tatgactttt tggcgttctt ggggtgacag tttgcctaca tcgcgtccgt
     4441 ccaacagggt ttctcccacc atctcgccga ctgccgcgcc gattgcgccg tcccgacatt
     4501 tgcctttatt tgctaccgct gccgcacagc ccgctatggc atgggcaatc ttgtgggcaa
     4561 tgtagtcttc gctgagatta aatttgattt tgctcgctac ttctccgtgt acggtactga
     4621 ctatcgcacc cagtgcggca tcgcccaagt tgtcttttag gctgccgccg ttgatggcgg
     4681 tatggacact tgcggcagcg gtgctgttga tcaggttagc ggtcagtttg cctgctgcgg
     4741 gactgtgaaa atgtttgctg acggcttcgg ctgcttgggt gttcagcccg cttatgccct
     4801 gcagtacgcc tgcggttacg gcggcggtgg cggcctgtct gacggtgctg cttttgccca
     4861 gttctttcag ggtatggttt atgtctcctt tgttgttgat gagggaaact gcggcttggc
     4921 tggcgagact ggccagtgcg gctttgcctg cggctgtggt gatagctgca gctgatgtgc
     4981 ctgctgctac tccggttgtg gctgccgttc ctgctgctac tccagttccg gctgccgttc
     5041 ctgctgcggc tcctcccgct cctgcgcctg ccgcgcccgc cgttccggcg gctgccgggg
     5101 cggacagtgc accgtaggtc aatacggtta cgacgataac gacgacagct gctgctgcgg
     5161 gtgtcatgcc ctcctgtttg tagtcccatt tatcgtaagc aagctgcacc tgattccagt
     5221 tgatgttttt cgcaacttga agttgtttca aataagcata ctcgggctgc ttggtgaggg
     5281 tttcgatttg ggttttcaga ttgcctttcg gaatgtcgac aatgtaaccg ccgggagcag
     5341 agagtacggg cgcaacgggg ccggtgaaac ttggcagttg cagggtttcg atgttgctgc
     5401 cgcgtcctgc ctgtttttgc cagagggcgg atttgctgct gcttacggtt tctgtgtgga
     5461 tgctgctttt gatcccttcg aggataatct tggcatcggc ccgtgcctgg tcgcctacgc
     5521 ctgcacggat gaccgcgccg cccagcgtgg tttcaaactg cgtaccttgt aacttggtat
     5581 cccaacctga ttgcagatta gctgattcag ccactacacg gcttggtaag gcagtttgca
     5641 tctgttgggt agaggtatca tgtaccttat cataagtaat gccgagaaat ttgcgtttct
     5701 tatgactgtc tagtttgtcg tagttgagct cttctacggc atagagtgtc aatttccgcc
     5761 cggcttcgat gttaatgctg cctttggggg catcgaatac ggtggcgtag gcgtcgatgc
     5821 tgccaccaga tttgatgtcg ataccttggg atgcgctgag gtttactgcg tcgggcttgg
     5881 cgtttttgtg ttctttgact tcggtaatgt gtttgcggtt gtaccatttg ccggttttgt
     5941 agctgcggcg ttctaaggta tagagttcgc cctgcccggc gtaataggat tggtcgccgt
     6001 aggattgcag tttgattttg ccgttttctg cggctatatc ggcggcactc aaaatgatgc
     6061 ggctgttctc attggcatac ggtgcgctaa tgctcacacc ggttttaccc gaaagttctg
     6121 cagcaagcgt agggggtgtc ttcagcacgc acgcgtctgg gcagtatata gggaatctga
     6181 atatttactt gaataacaaa tgccgtctga aaaattgtga gcttttcaga cggcattgag
     6241 ccgtaaatca tggaacgcgt gcgtgctgaa gcacacacct tacgcatgga ttttaggttt
     6301 catgcaggct acagcttgct gcggcggcgg tataaaagta tttttagtga tttaccgttt
     6361 ttggtaattt tgcccgacgg ggttaaaaat acagttgcta ctcttgctgc atgatattgg
     6421 caagatttaa tttaaatgtt tgttgaatct tgtttacatc cttagcaaaa atatgatagt
     6481 cactcgaaga atagatagat tttattctat ccaaattagt ttgttgataa cattttttca
     6541 tttccgcgat gaaaaaatta tcttttcctc tttcattgag acagttattt tctaacagat
     6601 agattgccca tgctgtaaaa ataaatgtac tatcttccgg atattcagat aaataaattt
     6661 cgaattcaat tatagaatat tcagctgctt caattaatgt tatatctaaa tctctgacaa
     6721 atttttccgt tatattttta aataatttct tgaattctat ataatttttt attttttcca
     6781 tgattctatt ttcctgtaaa aatatttaat tgaatatttg gatagcgatt tctaaactct
     6841 aaaataacat tgctgcaaga ttgacaggcc tttaattctg taaatagatc aattctacct
     6901 gatacattac gattatttcc gagttgctgt gctatagttt caagtaattt atattctcca
     6961 tctatatttc ttaatgttcc tctaggagga gaggcaatat tatgatattt atcaacagat
     7021 ataggtttaa aaatttttgt ttcaggtaac gaaataaatc catgttcccc tttttggaaa
     7081 gaactaaatg cttccatcct ttgcggtaat cctttaacat caatttttgc tatcgcaaga
     7141 ttccctattt gtcgatactc ttccggcagt ttcatgcgga ttctctggct ttcagcagtt
     7201 aaagcttgtc ttaaagcagg agcactgtca ttgctcagat aacgggtaac aggtgcaggc
     7261 ttggccgata ttgcagcctg tgcagataca gatcctgcaa ccatcaatgt gccattaaca
     7321 acctgaactg taccatgcag atattctcca ttagagattg ctttcgctcc ctgtccaatg
     7381 ttatatcctc ctgctgccac agtagctgct ttgacactga agccaagcgg tccgtttaat
     7441 aagtaaagtg caggttgtgc ggcaacagct gatttgtaat caaaagaatc ataacgactc
     7501 aatgtttgat aggctttcga taaggtgtta ttaatatctt tggaaggaat agtgagatta
     7561 cccattgact ggggcgtttt cagtatcgaa gctgcccaag ctgtgttacc tccagaggtt
     7621 tggttaataa aatatgcaag aatatttttt tcttgattat ttaaactact tggatttttt
     7681 tgatatcggt caagcaattg attagaatat cgatctttct caatcaaata gataaactgc
     7741 cttgcctctt gttcggtcag gctgccccaa tctaaagata ataggccttt ccctttggct
     7801 tttttgatca atttttctct aagagcatac tcttcttgag ataataggtt attttcaatc
     7861 gcgactttag ccgcattcgc cgctgtattc acatcgccgc cgaccacacc gcttaccgta
     7921 ccggcaacca gtttgctgta tgccaaaatc tgttcgcgtt ctttagctgt caaagtggca
     7981 ggatttttgc cgtttgtcaa agcctccccg actatctcgc ccacagccgc accgatcgca
     8041 ccatcctgac acttaccctt attcgccgcc gctgccgcac agcccgctat ggcatgggca
     8101 atcttgtggg taatgtagtc ttcgctgaga ttaaatttga ttttgctcgc tacttctccg
     8161 tgtacggtac tgactatcgc acccagtgcg gcatcgccca agttgtcttt gaggctgccg
     8221 ccgttgatgg cggtattaat cagtgcggca ctgcccgcat tggctaggtt gacggtcagg
     8281 ttgttgatcc actgcttatc gctgacattg ttcagtgccg aagcgccgat tttgtcggct
     8341 acgcctgcgg tagcggcggc aaccaccaga tttttcaccg tgctgcttct gcccagctct
     8401 ttcagggttt tgccgacatc gcctttgttg ttgatgaacg atacggaagc ctggctggcc
     8461 aaagaggcga atgctgcatc ggttgcggcg gcggccgcac cgtttaatcc caatacggct
     8521 ccggttcctg cgcctgaggt gaccacggta acggccagtg cgataatcgc cgcacctgct
     8581 tcggttaagc cctcctgttt gtagtcccat ctgtcgtaag caagctgcac ctgattccag
     8641 ttgatgtttt tcgctacttg gagctgtttc agataggcat actcgggctg tttggccagc
     8701 ttttcgattt cggttttgag gttgcctttg gggatgtcgg cgatatagcc gccgggagcg
     8761 gtcagcttag gcagtgccgg cccttcaaag ctcggtagct tcagcgtttc aaccgtgctg
     8821 ccgcttccgg cctgcttttg ccatacggtc gagttggatt ccagcttttc ttcggtttgg
     8881 atgcggttaa cgatgccttt taggataatt ttcgcatcgg ctcgggcttt ttcacccacc
     8941 cctgcctgta tgtcggctcc ggaaagggtg gttttgaatt cggtgccttc gagtacggta
     9001 tcccagccgg aacgggtttt ggctgtttgg gcgataacgc gtacgggcag tttggtttcg
     9061 ttcagctcgt ttttgctgta attgctttta cccactttga tgccgatgaa acgggtactt
     9121 ttctgaacat tcaattggtg ttggtgaatg ccttcggctg ccagcagttg cattgaggcc
     9181 gtctgaaaaa cggatattca gacggcctat tgctacttta aataatattt gggtttaaaa
     9241 tatcatcgta tttcccaaat tcggggaaca gaaaatcaat tatttaaatt tttcatcctc
     9301 ataatatggg tgatcaaaaa gctctccttc ccacatattt atttcttcta tgcctttatc
     9361 tttcataaac tcatgataat cataaaaatc ttttccatat tgtccgtaaa aaattttcat
     9421 atccccaagc cacatagcat aacattcatc gcataaaatg acctccccag agaaatattt
     9481 aggctttgca tgataaaggt agccttgctc acatcttggg caaattttaa tttctttatc
     9541 cataagagat tcctttctta attatttctg aggatatgca gaaatgattt tatttgttcc
     9601 tttttgcaca acaatccgca cttttgtttc tccttttgtt ccaataggtt tcttcatatc
     9661 tacaagatat acggatgaat catttggcaa aggatttcct ttcattctcc aagcttcatc
     9721 aaccaaaggc aaaacttctt tacgggacac attaaaaaca gaatgaacag gtttgtttgg
     9781 atttggaatt gtatgggcta aaacatgttt aatcctatta ccatgcttag catctaaccc
     9841 ataatctaac cccgccggtg ttgtccaaac cttattaacc ttatcataag taagcttgat
     9901 tccggtctgc ttctgaattt cctgtatggc tttagccgta tcaactgctt cgtctcccgc
     9961 cttcagaaca taagccccgc ctcggctgat tttagtcgca cctgccggaa caaagggaac
    10021 ggcagctgcg gcggcatcca caccaaaatc aatcgccgct tctcgagcca tctgcttatc
    10081 gccaatagat ttggcgtaaa tccatttacc gccgtcccat acgaggttgc cgatatccca
    10141 aataatatct aatgcattat ttttaacagc tgtttcggaa gcatttgccg ccacatcaac
    10201 attgcctcct gtaacaccta cagtcgctcc ggctaccaat tttgcatacg cagtaatatt
    10261 cgctttagct ttttcaattt cagaagcagt cataccgctg aaatcggtat tcttaaccaa
    10321 agcctccccg acaatctccc ccacgaccgc accaatcgcg ccgtcctgac acttgccctt
    10381 attcgccgcc gctgccgcac agcccgctat ggcatgggca atcttatggg caatgtagtg
    10441 ctgatccaat tgtttgattt tacttgctgc ctctccatgc gcagtattca ccaaagccgc
    10501 aaggatattc gcttccagat tgtctttcag actgccgccg ttaacggcgg tgttaatcag
    10561 cgcggcactg cccgcattag ccaagttgac gttgaggttg tttacccaag gggtttcgct
    10621 ccaagtggca agggaggaag caccgatttt gttggatacg cctgcgatgg ccgctgctac
    10681 aaccagtttt ttcactgtgc ggcttctgcc cagctccttc agggttttac cgacatcgcc
    10741 tttattgttg atgagcgata cggaagcctg gctggccagc gaggcgaatg cggcatcggt
    10801 tgccgctgcg gccgcgccgt ttaagcccag tgcggctccg gctcccgcgc ccgcagtaac
    10861 cacggtaaca gccagcgcaa taatcgctgc tccggctccg gttaagcctt cctgtttata
    10921 gtcccatttg tcgtacgcca gttgtacctg gttccagttg acgtctttgg tgacttggag
    10981 ctgtttcaaa tagcaagcgt agggggtgtc ttcagcacgc acgcatctgg gcagtatata
    11041 gggaatctga atatttactt gcataacaaa tgccgtctga aaaaattgag cttttcagac
    11101 ggcattgagc cgtaaatcat ggaacgcgtg cgcgctgaag cacacacctt acgcatggat
    11161 tttaggtttc atgcaggcta cagcttgctt ccataaatca tttttatcag agctcgtagg
    11221 tacggttagc cgcctttagc ggcgtaaccg tacgaatgaa atgccaagtt gcaaggccgt
    11281 ctgaaaaagt tgaaaaacag atttcagacg gccttgttat tttataaagt ttgctgatat
    11341 gcgtacggtt acgccgctaa aggcggctaa ccatacctac gcttgctcat aaatatcaat
    11401 attcggcaaa tcggccaaat ctattggaca cgcaatatcc caccaaagcc attctaagta
    11461 ataccaaggg tcttcaggcc atattgcttg ggcatcttcc aaagtaggcc atatgtcttt
    11521 caatttctgc acttgttctt ttgaagttcc agttagagga attccgatac cgtcggtata
    11581 atcatgtaaa cggattgaac cgtcatttaa cagttcttcc ataaaagaac agaaattgtt
    11641 ttttaagttt ttatcttgaa tattaatacc catttgattt ttataagagt taaaaattga
    11701 ggataaatcg atttgaaaat caagaatctt aattttttgt ttctcatcca cggtaaaatc
    11761 ctttgtaaat tattggaatt taagctccaa tttagtacct ttaataaatg atggaggatt
    11821 ctgcaaatct aatgtccatc gtgcttgagt tgaattctct gtttttgaaa aatttcttaa
    11881 tgcaattttt gttcctgccc attctccagt actgataatg ccatttgcta atcttccgtc
    11941 aggcaaaact ctaaaattcg gattctggcc agtcatctgc cgataaagcg caaaaatctc
    12001 ctgttcgctt acgccgctgt aaacgggtgc tccctttaag cttatggctt gtacaggttt
    12061 gatggttttt ccgttaatgg ttatcggaat attacttgca tcaagcaagc ctgctccttt
    12121 ccttactcta ttgttaaatc ccgtcttcca tgcatccagt tgcgtatcgc gttgggcaat
    12181 acggaataaa atctgctgtt ctgctttggc taaatttgcc aaattgttta ttgtttcttt
    12241 aggagcagc
//
LOCUS       DAWKST010000057        11563 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.057, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000057 DAWKST010000000
VERSION     DAWKST010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11563
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.057"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(109..1152)
                     /locus_tag="WJL87_001047"
     CDS             complement(109..1152)
                     /locus_tag="WJL87_001047"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ7799478.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIWDIPTWEHWYGMPHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEVGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETILRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLGAIPLLP"
     gene            complement(1149..1430)
                     /locus_tag="WJL87_001048"
     CDS             complement(1149..1430)
                     /locus_tag="WJL87_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799479.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPAGFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQRSDGIPANHTKEKP"
     gene            1740..2840
                     /locus_tag="WJL87_001049"
     CDS             1740..2840
                     /locus_tag="WJL87_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ7799480.1"
                     /translation="MYQKPKLFKGAVRNLEAASWKDINEIYQRADPTAPLFYLRKKGA
                     IVPKEEYVESFDALGKTRYRFIKSVIYEHMKNGASLVYNHINNEPFSDHISRQVARLA
                     GAHTIVSGYLAFGSDESYKNHWDTRDVYAIQLFGKKRWQLTAPDFPMPLYMQQTKDTD
                     ISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHLAVGTFPPNGYNYLEWLMKK
                     FPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEAFSEDFLGKERTDTAFHLE
                     QFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISVDELGKKVLEHIGKNEPLL
                     LKNLLVNFNQGKYEEVRKLIYQLIELDFLEIL"
     gene            3069..3422
                     /locus_tag="WJL87_001050"
     CDS             3069..3422
                     /locus_tag="WJL87_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799481.1"
                     /translation="MIELQLHELKLVSGGGPVTDNIAGNVANAATTKGGPTWGDLVAI
                     PAAAAAVYYLPKNAYGAAGANGVYNVTRDWVNDAVNAPPYNGRPIFEIEHGLTAPATK
                     ADKSGNGYTDGTDYC"
     gene            3483..4109
                     /locus_tag="WJL87_001051"
     CDS             3483..4109
                     /locus_tag="WJL87_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HEZ7799482.1"
                     /translation="MNRPKQPFFRPEVAVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTAEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHGNETRSLKATVERLENQKLH
                     ISQQIDGQKRRIRLAEDQWQKRHWADRNCRDWAWYPPIWRRATNLFIS"
     gene            4061..4261
                     /locus_tag="WJL87_001052"
     CDS             4061..4261
                     /locus_tag="WJL87_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799483.1"
                     /translation="MVSSYLAKSNEPVYLVKIKPDKPTITAYGEEKPLQIGMTLEADI
                     LHEKRRLYEWVLELIYSMSGKL"
     gene            4571..4873
                     /locus_tag="WJL87_001053"
     CDS             4571..4873
                     /locus_tag="WJL87_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799484.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
     gene            5157..5396
                     /locus_tag="WJL87_001054"
     CDS             5157..5396
                     /locus_tag="WJL87_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ7799485.1"
                     /translation="MPHLQNLSLGLKKKLPVILQTEISECGLACLAAVAGFHGFHTNL
                     RALRSKYCPRPLQNSPKSPKCLGGNFGEFCKGLIL"
     gene            5457..7199
                     /gene="hrpB"
                     /locus_tag="WJL87_001055"
     CDS             5457..7199
                     /gene="hrpB"
                     /locus_tag="WJL87_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HEZ7799486.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTARKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISTFNNKFPLYRNKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEGKS
                     DLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLAHK
                     TDLTDATGTETASGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQY
                     QSSLAAERMLWRNRLHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYL
                     NRWQLDGKLSYKRGTGMRQSMPAPEENGGDILPGTSRMKIITAGLDAAAPFILGKQQF
                     FYATAIQAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLFGERGFYWQNTLTWYFHPNH
                     QFYLGADYGRVSGESAQYVSGTQLMGAVVGFRGGHKVGGMFAYDLFAGKPLHKPKGFQ
                     TTNTVYGFNLNYSF"
     gene            7308..>11563
                     /locus_tag="WJL87_001056"
     CDS             7308..>11563
                     /locus_tag="WJL87_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEZ7799487.1"
                     /translation="MNKGLHRIIFSKKHSTMVAVAETANSQGKGKQAGSSVSVSLKTS
                     GDLCGKLKTTLKTLVCSLVSLSMVLPAHAQITTDKSAPKNQQVVILKTNTGAPLVNIQ
                     TPNGRGLSHNRYTQFDVDAKGAVLNNDRNNNPFLAKGSAQLILNEVRGAASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGAPQIGKDGALTGFDVRQGTLTVGA
                     AGWNDKGGADYTGVLARAVALQGKLQGKNLAVSTGPQKVDYASGEISAGTAAGTKPTI
                     ALDTTALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADG
                     TEASPTYLSIETTEKGAAGTFISNGGRIESKGLLVIETGEDISLRNGAVVQNNGSRPA
                     TTVLNAGHNLVIESKTNVNNAKGSANLSAGGRTTINDATIQAGSSVYSSTKGDTELGE
                     NTRIIAENVTVLSNGSIGSAAVIEAKDTAHIESGKPLSLETSTVASNIRLNNGNIKGG
                     KQVVLMADGDIQAKASNLNASGHLYIHAGKDLDLNADKDLSTQSISLRADNTALISSN
                     GNTLTAEKNLDIQAGSLSVRQSNLQSSGGNVQMSATKGNISLNQSWINASQNIDTAAL
                     QGNIISDGLTAVAEVGRVSLLANGNVDFNGLNTLIAEGDINAGSVGKGRLKMDNTDIY
                     ASAGDVKLVAGGQLDLGNGTVNGGHISLDSNKGSMVVQDVHLNARASLKVDADQTLTI
                     NNSKLNSGHNTQINTNHGHMTLNQLDAHSRRHMSISAQGKGKGKDSGQILQNDQQNSK
                     STLAADGVLSLNSSALQVLDNTTLRGGAINIKAGGGIIKRGYIDWETQDTATMRSAEL
                     KPLSGMMSIESGGDNPLTVEPGNRIVSAGDLAVNHNGTFQISARAGNNGNPSAQTASV
                     SAKGNIGIVAGEVDIDAANIAAGKDLALVATKGNISLNSIRNTFSNYQLKTDKHNITQ
                     QLTDVEQELSKLTSDPKYRKAQDLPQMLRRKYKRRDKVFGDSEARLRGLRAEINAADE
                     AWAELQSPVKALLERKQLLQQALLTVSQPGSGHENQGSTLSGQNIKLLAAGGIRIQGS
                     KVAATQQANIQAAGFLPAPAAEELQEGRLQSAIDISGVFDTFEYGQQGSDKYGYAIFS
                     RPSEISGKTGVTLSAPNANENSRISLSAANIEAENGKIKLQSYGDQSYYAGQGELYTL
                     ERRSYKTGKWYNRKHITEVKEHKNAKPDAVNLSASQGIDIKSGGNIDAYATAFDAPKG
                     SINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYSKAHDTTTQVMKTALPSRVVAE
                     SANLQSGWDTKLQGTQFETTLGGATIRAGVGEQARADAKIILEGIKSSIHTETVSSSK
                     STLWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYIVDIP"
BASE COUNT         3294 a         2657 c         2825 g         2787 t
ORIGIN      
        1 tttcgtggga atggatgggc ccaaagccgt ccgaaccaaa aaaaaccgcc gctttcagac
       61 ggcatcaaaa aaccgcaggc acgatgcctg cggtttgccg tttgcgcttc aggggagcag
      121 ggggattgcg cccaagccgt gtgtttcttt cagtccgaac atgatgttca tattttgcac
      181 cgcctgaccc gccgcgcctt tgacgaggtt gtcgatgacg gaaaggacga cccacacatc
      241 ggattgcgcc gcctgttgga tgctgatgcg gcagaggttt gcgccgcgca cgctgcgggt
      301 ttctggtgtc gaaccggcgg gcaggatgtc cacgaacagg ctgtcgcggt aatagtcgcg
      361 caggatggtt tcggggcaga tgccgtctga aaggtggagg taaacggtgg cgtgcatacc
      421 gcgtatcatc ggcgcgaggt gcggcgtgaa cacgaatcct tcggcgatgc cgtcctgaag
      481 cccggcgatg gtctgcctga tttcgggcag gtggcggtgt ccggctatgc cgtaggcttt
      541 gaagttgtcg ccgacttcgc acaacagcga accgacattg cctttcctgc ccgcgccgga
      601 cacgccggat ttgcagtcgg caatcagcgg cataccgggc ttcagacggc attgccgcaa
      661 cagcggcacg agcggcaggg atacgcaggt cgggtagcag ccggggttgg cgacgaggcg
      721 cgcctgtgcg acggcttcgc ggttgagttc gctcaatccg tacacggctt gggaaacgag
      781 gccgggggcg gcgtggggca tgccgtacca gtgttcccag gtcggaatgt cccatatgcg
      841 gaagtcggcg gaaaggtcga tgacgcgcac gccctgttca atcaggcgcg gcgcgtcttt
      901 catggcgatg ccgttgggcg tggcgaagaa gacgatgtcg cattgttcca aacctgcctc
      961 gtcgggcgtt tggaaggcga ggccgtacac gccgcgcaaa ctcggaaagt aatcggcaac
     1021 tgcggttccc gcttcgctgc ggctggttac ggcggcgact tcgacatcgg gatgggcggc
     1081 aagcaggcgc agcagttcca cgccggtgta gcccgtcgcg ccgacaatgc cgactttgat
     1141 ttttttactc atggtttttc ctttgtgtgg ttggcgggta tgccgtccga acgctgtccg
     1201 acggggtttt gattggcggc ggaaggacgg tacacaaagc gtttgaaaag cgtgtctaat
     1261 ttaatggcat ccgcctctgt ttttaaaacc cagccctgcc gtccggaata aacataaccg
     1321 taggcgcgaa agccggcagg atatttcggt gcctgcccca aacccaaacc gtatgcggcg
     1381 aaagcggtgg tcgtggagaa ggaaagcagg aggaggacga gggttttcat aaggttccaa
     1441 accgaacggg gcgatgaagc ccgattatag caaaaggtcg tctgaaaacc gttttcagac
     1501 gaccttttat ttaattacaa taaaaatgtt aacaacaaaa aacaaaccgc ttttttccgt
     1561 ttggagacaa atttttaact aaaattgtca acaattcctt gacgcacaca caaaaaaaaa
     1621 ctttaatatt tgtaaatata tgtaacatta taaattttaa atattttctg agtaagggga
     1681 agtaatgagc atccatctcg acttcggcat tagtcctaaa acgttccgac agacttaatc
     1741 tgtatcaaaa gcccaagctc tttaaaggag cggttcggaa tctcgaagcc gcatcttgga
     1801 aagatatcaa cgagatatac caacgagcag acccaaccgc accgctgttt tatctgcgta
     1861 aaaaaggtgc aattgttcct aaagaagaat acgtcgaaag tttcgacgct ttgggcaaaa
     1921 ctcgctaccg ttttattaaa tccgttatct acgaacatat gaagaatggt gcgtcgttag
     1981 tctataacca tattaacaac gagccgtttt cagatcatat ttcccgtcaa gtcgcccgcc
     2041 ttgccggcgc acatactatt gttagtgggt atcttgcttt tggcagcgac gaatcttata
     2101 aaaaccattg ggacacccgc gatgtgtatg ccatccagct tttcggcaag aaacgttggc
     2161 aacttactgc ccctgatttc cctatgccat tgtatatgca acagactaaa gatactgata
     2221 tttccattcc tgaacatatc gatatggata ttatccttga agcaggtgat gtcctctaca
     2281 tcccgcgcgg ttggtggcac agacctatcc cgctcggctg tgaaaccttc cacctcgctg
     2341 tcggtacctt tccgcccaac ggctataatt acctcgagtg gctaatgaag aaattcccca
     2401 ccatagaaag tctgcgccac agtttctcag actgggagca agataggacg cgtatcaacg
     2461 atactgccgc acaaattgct gccatgattg ccgaccccgt caattatgaa gccttcagtg
     2521 aagactttct cggcaaagaa cgtaccgata ccgcttttca tctcgaacag ttcgcgaatc
     2581 ccaacgctac tccgctttca gacgacgtca ggttgagatt aaatgccaat aatttggata
     2641 cgttggaaaa gggatatttg attgggaatg ggatgaagat aagcgtagat gagttgggga
     2701 aaaaagtttt agaacacatc ggtaagaatg aaccgttatt gttgaaaaat ctactggtta
     2761 acttcaatca gggaaaatat gaagaagtta ggaagttgat ttatcagttg atagagttag
     2821 attttctgga aattttgtga gggattctat gaaaaactgg aagcaattta catttttcgt
     2881 aatattagta atagcttgtt atcaagtgct atatttttta tcagatatgt ttcttctcga
     2941 ctacattaac aaatatagtt ggaatttgaa ttttattcag ggtactttga attttttttc
     3001 aatatatctg ccatatgttt ttgttagccg aatttttagg aataccaacc aagaaaagga
     3061 gtataaaaat gattgaactt caacttcatg aattgaagct ggtttcaggg ggaggtcctg
     3121 taacagacaa tatagctgga aatgtagcta atgctgccac aaccaaagga ggtcccacat
     3181 ggggggattt ggttgcaata cctgctgccg cagcggcagt ttattatctg ccgaaaaatg
     3241 cttacggtgc tgctggtgca aatggtgtat acaatgtgac tcgtgattgg gtaaatgatg
     3301 ccgttaatgc acctccttat aacggaagac caatctttga gattgaacat ggattaactg
     3361 ctcccgcaac aaaagcagat aaatcaggaa acggctacac tgacggtaca gattactgct
     3421 gatatttctc attgttcaga cgatctctag gggtcgtctg aaactttctt aacttcaatt
     3481 ttatgaatag acccaagcaa cccttcttcc gtcccgaagt cgccgttgcc cgccaaacca
     3541 gcctgacggg taaagtgatt ctgacacgac cgttgtcatt ttccctatgg acgacatttg
     3601 catcgatatc tgcgttattg attatcctgt ttttgatatt tggtaactat acgcgaaaga
     3661 caacagcgga gggacaaatt ttacctgcat caggcgtaat cagggtgtat gcaccggata
     3721 cggggacaat tacagcgaaa ttcgtggaag atggagaaaa ggttaaggct ggcgacaagc
     3781 tatttgcgct ttcgacctca cgtttcggcg caggaggtag cgtgcagcag cagttgaaaa
     3841 cggaggcagt tttgaagaaa acgttggcag aacaggaact gggtcgtctg aagctgatac
     3901 acgggaatga aacgcgcagc cttaaagcaa ctgtcgaacg tttggaaaac cagaaactcc
     3961 atatttcgca acagatagac ggtcagaaaa ggcgcattag acttgcggaa gatcagtggc
     4021 aaaaacggca ctgggcagac aggaattgtc gggattgggc atggtatcct cctatttggc
     4081 gaagagcaac gaacctgttt atctcgtgaa aataaaaccc gacaaaccaa ccatcactgc
     4141 atacggtgag gaaaaaccgc tgcaaatcgg catgacgttg gaagcagaca tcctgcacga
     4201 gaaacggcgg ctgtacgaat gggtattgga gctgatttat agtatgtcgg gcaaactgta
     4261 agacttgagg ctgaaagata aagaagttgt ctgaaacctt ttcagacgac cagaagacaa
     4321 taaaaaacac aaacttcaaa actgcctgaa accatcccct tttacaggca ctccgtttgt
     4381 aaaagcaagg atagggcatc tgcccttttg attgtttgat taacgataca aggagtttca
     4441 aaatgagagt tttatagtgg attaacaaaa accagtacag cgttgcctcg ccttgccgta
     4501 ccatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt taaatttaat ccactatatg
     4561 tgttcatgaa atgacttggg tcggaggctc aggtaatgcg caacaaagct catattattg
     4621 cgaaatttgc gaatctgcag ggcttaacga tacgggaaat cctgataaat ctttaggatt
     4681 gccaaacaat acgttcagta atccgcctgg ttggggagct acaatcggag ctttagcagg
     4741 tagccgcata gggatgcctg aatttggtac gtttgcgagc catgccattg aaaatttcga
     4801 ctggtcatgg tatcgacgtt atagggaaat tgccgaaacg attgaacgag aatattcagg
     4861 cggtttgcct taatagttga ggaggtcatg atgtttgcca aacattatca attcatcgca
     4921 ctcggcatca tgctgcttct ttatatgttg attctctata cgaccgattt ttccaatctg
     4981 acgtattgga tgctgttttt tatctgtttt attacaggaa aaatattagc tcgtttgtta
     5041 gagaaaagct ttaaataaaa tagcagctag tcgcaaaagg tcgtctgaaa ccttttcagg
     5101 tggcctttct aaaatacatc caacttccta atccctattt ttcaaaaagg aaatctatgc
     5161 cccatctgca aaacctgtct ttgggcttaa agaaaaagct gcctgttatc ctgcaaacag
     5221 aaatatcaga atgcggcttg gcatgtctgg cggctgtggc gggatttcat ggtttccata
     5281 cgaatttacg cgcactgcgt tcaaaatact gtccgagacc tttgcaaaat tccccaaaat
     5341 cccctaaatg tcttggtggg aattttgggg aattttgcaa aggtctcatt ctataactgt
     5401 aaatactttt aaatttatga caaaatagta aatattgcta aaataatatt gatgtcatga
     5461 aattttttcc tgctccatgt ctgttggtta tcctggctgt catacccctt aaaaccttag
     5521 ctgccgatga aaacgatgca gaacttatcc gttccatgca gcgtcagcag cacatagatg
     5581 ctgaattgtt aactgatgca aatgtccgtt tcgagcaacc attggagaag aacaattatg
     5641 tcctgagtga agatgaaaca ccgtgtactc gggtaaatta cattagttta gatgataaga
     5701 cggcgcgcaa attttctttt cttccttctg tgctcatgaa agaaacagct tttaaaactg
     5761 ggatgtgttt aggttccaat aatttgagca ggctacaaaa agctgcgcaa cagatactga
     5821 tcgtgcgtgg ctacctcact tcccaagcta ttatccaacc acagaatatg gattcgggaa
     5881 ttctgaaatt acgggtatca gcaggcgaaa taggggatat ccgctatgaa gaaaaacggg
     5941 atgggaagtc tgccgaaggc agtattagta cattcaataa caaatttccc ttatatagga
     6001 acaaaattct caatcttcgc gatgtagagc agggcttgga aaacctgcgt cgtttgtcga
     6061 gtgttaaaac agatattcag attataccgt ccgaagaaga aggcaaaagc gatttacaga
     6121 tcaaatggca gcagaataaa cccatacggt tcagtatcgg tatagatgat gcgggcggca
     6181 aaacgaccgg caaatatcaa ggaaatgtcg ctttatcgtt cgataaccct ttgggcttaa
     6241 gcgatttgtt ttatgtttca tatggacgcg gtttggcgca caaaacggac ttgactgatg
     6301 ccaccggtac ggaaactgca agcggatcca gaagttacag cgtgcattat tcggtgcccg
     6361 taaaaaaatg gctgttttct tttaatcaca atggacatcg ttaccacgaa gcaaccgaag
     6421 gctattccgt caattacgat tacaacggca aacaatatca gagcagcctg gccgccgagc
     6481 gcatgctttg gcgtaacaga cttcataaaa cttcagtcgg aatgaaatta tggacacgcc
     6541 aaacctataa atacatcgac gatgccgaaa tcgaagtgca acgccgccgc tctgcaggct
     6601 gggaagccga attgcgccac cgtgcttacc tcaaccgttg gcagcttgac ggcaagttgt
     6661 cttacaaacg cgggaccggc atgcgccaaa gtatgcctgc accggaagaa aacggcggcg
     6721 atattcttcc aggtacatct cgtatgaaaa tcattactgc cggtttggac gcagccgccc
     6781 catttatttt aggcaaacag cagtttttct acgcaaccgc cattcaagct caatggaaca
     6841 aaacgccgtt ggttgcccaa gataaattgt caatcggcag ccgctacacc gttcgcggat
     6901 ttgatgggga gcagagtctt ttcggagagc gaggtttcta ctggcagaat actttaactt
     6961 ggtattttca tccgaaccat cagttctatc tcggtgcgga ctatggccgc gtatctggcg
     7021 aaagtgcaca atatgtatcg ggcacgcagc tgatgggtgc agtggtcggc ttcagaggag
     7081 ggcataaagt aggcggtatg tttgcttatg atctgtttgc cggcaagccg cttcataaac
     7141 ccaaaggctt tcagacgacc aacaccgttt acggcttcaa cttgaattac agtttctaac
     7201 ctctgaattt tttactgata tttagacggt ctttccttat cctcagaccg tcaaacttta
     7261 cctacgtact tggcgcgcag tacgttcatc ttcaaaatgg aatagacatg aataaaggtt
     7321 tacatcgcat tatctttagt aaaaagcaca gcaccatggt tgcagtagcc gaaactgcca
     7381 acagccaggg caaaggtaaa caggcaggca gttcggtttc tgtttcactg aaaacttcag
     7441 gcgacctttg cggcaaactc aaaaccaccc ttaaaacctt ggtctgctct ttggtttccc
     7501 tgagtatggt attgcctgcc catgcccaaa ttaccaccga caaatcagca cctaaaaacc
     7561 agcaggtcgt tatccttaaa accaacactg gtgccccctt ggtgaatatc caaactccga
     7621 atggacgcgg attgagccac aaccgctata cgcagtttga tgtcgacgct aaaggtgctg
     7681 tactgaacaa cgatcgcaac aacaacccgt ttctggccaa aggcagtgcg cagttgattt
     7741 tgaacgaggt gcgcggtgcg gccagcaaac tcaatggcat cgttaccgta ggtggtcaaa
     7801 aggccgatgt gattatcgcc aaccccaacg gcattaccgt gaacggcggc ggctttaaaa
     7861 acgtcggccg cggtatctta actaccggtg cgccccaaat cggcaaagac ggtgcactga
     7921 caggatttga tgtgcgtcaa ggcacattga ccgtaggagc agcaggttgg aatgataaag
     7981 gcggagccga ctacaccggg gtacttgctc gtgcagttgc tttgcagggg aaattacagg
     8041 gtaaaaacct ggcggtttct accggtcctc agaaagtaga ttacgccagc ggcgaaatca
     8101 gtgcaggtac ggcagcgggt acgaaaccga ctattgccct tgatactacc gcactgggcg
     8161 gtatgtacgc agacagcatc acactgattg ccaatgaaaa aggcgtaggc gtcaaaaatg
     8221 ccggcacact cgaagcggcc aagcaattga ttgtgacttc gtcaggccgc attgaaaaca
     8281 gcggccgcat cgccaccact gccgacggca ccgaagcttc accgacttat ctctccatcg
     8341 aaaccaccga aaaaggagcg gcaggcacat ttatctccaa tggtggtcgg atcgagagca
     8401 aaggcttatt ggttattgag acgggagaag atatcagctt gcgtaacgga gccgtggtgc
     8461 agaataacgg cagtcgccca gctaccacgg tattaaatgc tggtcataat ttggtgattg
     8521 agagtaaaac taatgtgaac aatgccaaag gctcggctaa tctgtcggcc ggcggtcgta
     8581 ctacgatcaa tgatgctact attcaagcgg gcagttccgt gtacagctcc accaaaggcg
     8641 atactgaatt gggtgaaaat acccgtatta ttgctgaaaa cgtaaccgta ttgtccaacg
     8701 gcagtattgg cagtgctgct gtaattgagg ctaaagacac tgcacacatt gaatcgggca
     8761 aaccgctttc tttagaaacc tcgaccgttg cctccaacat ccgtttgaac aacggtaaca
     8821 ttaaaggcgg aaagcaggtg gtactgatgg ctgatggcga cattcaggca aaagcctcca
     8881 atctgaatgc atccggccat ctgtatattc atgcaggtaa ggatctggat ttgaatgccg
     8941 ataaagactt atcaacacaa agtatcagcc tgagggcaga caacaccgcc ctcatcagta
     9001 gcaacggtaa caccttgact gcagaaaaga atctggatat tcaggcaggt agcttgagtg
     9061 tgcgtcaaag taatctgcaa tccagtggcg gtaatgtgca gatgagcgcg actaaaggca
     9121 acatcagctt gaatcagagt tggataaatg ccagtcagaa tattgatacg gcagcacttc
     9181 aaggcaatat tatttcagac ggtctgactg ctgttgccga agttggacgc gtatctcttc
     9241 ttgccaacgg caatgttgat ttcaacggtc taaatacctt gattgcggaa ggagacatta
     9301 atgctggttc agtcggtaaa ggccgtctga aaatggacaa taccgatatt tatgcttctg
     9361 caggcgatgt gaaactggtt gccggaggtc aattagattt aggcaacggc accgttaacg
     9421 gcggtcatat cagcttagac agcaataaag gcagcatggt ggtgcaggat gtacacctga
     9481 atgcccgcgc atcactgaaa gtggatgcgg atcaaacttt aaccattaat aacagcaagc
     9541 tcaattccgg tcacaatacc cagattaata caaatcatgg tcatatgacg cttaatcagc
     9601 ttgatgctca ttcacgtcgt catatgagta tcagtgcaca gggtaaaggc aaaggaaaag
     9661 acagcggtca aattttacaa aacgaccagc aaaacagtaa aagtactttg gcggcagatg
     9721 gtgtattgtc attgaacagc agcgcattac aggttttgga caatactacc ctgcgcggtg
     9781 gcgcgataaa catcaaagct ggcggaggca tcatcaaacg gggctatatc gattgggaaa
     9841 cccaagatac ggctactatg cgttcagcgg aactgaaacc actgtctggt atgatgtcga
     9901 tagaatcggg cggtgataac cctttaaccg ttgaacccgg taaccgtatc gtttctgcag
     9961 gtgatttggc cgtgaaccac aacggcacat tccaaatcag tgccagagca ggaaataacg
    10021 gcaatccgag tgcgcaaaca gccagcgttt cagctaaagg caatatcggg attgtggcgg
    10081 gagaggtgga tattgatgca gcaaatattg ccgcagggaa agatttggct ttagtggcaa
    10141 ctaaagggaa tatttcacta aacagtatta ggaatacgtt tagtaactat caattgaaaa
    10201 cagataaaca caatatcaca cagcaactta ctgatgtaga gcaggagctt agtaagttaa
    10261 cgagcgatcc taaatatcgt aaagcacagg atcttccaca aatgttgagg cgtaaatata
    10321 agaggcgtga caaagtattc ggtgatagtg aggccagatt acgtggttta cgagcagaga
    10381 taaatgctgc tgatgaggct tgggcagaac tccaatcccc agtaaaagcg ttacttgaac
    10441 gtaaacaatt gttacaacaa gcccttttaa cagtttccca accgggtagt ggtcacgaaa
    10501 accaaggtag cacccttagc ggtcaaaata tcaaactgct cgctgcaggt ggtatccgta
    10561 tccagggctc caaagttgct gccacccaac aggcaaatat ccaagctgca ggatttttac
    10621 ctgcaccagc cgcagaggaa ctgcaagaag gtcgacttca atcagccatt gacatcagcg
    10681 gtgtatttga tacttttgaa tatggtcagc aaggcagcga taaatacggc tatgccattt
    10741 tcagcaggcc atctgaaatt tcaggaaaaa caggtgttac cctctctgca ccaaatgcca
    10801 atgagaacag ccgcatcagt ctgagtgcgg caaacatcga agctgaaaac ggcaaaatca
    10861 aactgcaatc ctacggcgac caatcctatt acgccgggca gggcgaactc tataccttag
    10921 aacgccgcag ctacaaaacc ggcaaatggt acaaccgcaa acacattacc gaagtcaaag
    10981 aacacaaaaa cgccaagccc gacgcagtaa acctcagcgc atcccaaggc atcgacatca
    11041 aatctggtgg caacatcgac gcctacgcca ccgcattcga tgcccccaaa ggcagcatta
    11101 acatcgaagc cgggcggaaa ttgacactct atgccgtaga agagctcaac tacgacaaac
    11161 ttgacagcca aaaaaggcgc agatttctcg gcatcagcta cagcaaagca cacgacacca
    11221 ccacccaagt catgaaaacc gcgctgccct caagggtagt tgcagaatca gccaacctcc
    11281 aatcgggctg ggataccaaa ctgcaaggca cacagtttga aaccacactg ggtggcgcaa
    11341 ccatacgcgc aggcgtaggc gagcaggcac gggcagatgc caagattatc ctcgaaggga
    11401 tcaaaagcag catccacaca gaaaccgtga gcagcagcaa atctactcta tggcaaaaac
    11461 aggcaggacg gggcagtaac atcgaaacct tgcaattgcc gagtttcacc ggtcccgttg
    11521 cgcccgtact gtccgctccc ggcggctata tcgtcgacat tcc
//
LOCUS       DAWKST010000058        11533 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.058, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000058 DAWKST010000000
VERSION     DAWKST010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11533
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.058"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            121..543
                     /locus_tag="WJL87_001057"
     CDS             121..543
                     /locus_tag="WJL87_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799488.1"
                     /translation="MKKTFLSAVLLLSAATQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGWGRGRWATMKFQNGEFMYTVDAGFDSV
                     THAVGSGVLVERRGKEIARFDCTSKTAQANFDDDDFAW"
     gene            582..1322
                     /locus_tag="WJL87_001058"
     CDS             582..1322
                     /locus_tag="WJL87_001058"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ7799489.1"
                     /translation="MLIVRPSGRAKDDVETCRRAEWQAEVLSPIEIETDKASLKRLPE
                     MYARADAVFWVSPTAVETAVPYLNLSDGIKMHISVGQGSRRALERCLGRTVIAPDDGN
                     DSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFRRHKPLNF
                     QNFQTENIAAAYITSAELVQSLFAQVPPQFSRFFKSLLYFTHHPRIAEALKREGVCSV
                     ETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            1337..2671
                     /locus_tag="WJL87_001059"
     CDS             1337..2671
                     /locus_tag="WJL87_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ7799490.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLMLDGVLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKTIEAPSLPSERKPEQPAKKQPVPEKAGRSPSAKGERA"
     gene            2668..3885
                     /locus_tag="WJL87_001060"
     CDS             2668..3885
                     /locus_tag="WJL87_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ7799491.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVFNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLVNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETH"
     gene            3960..5024
                     /locus_tag="WJL87_001061"
     CDS             3960..5024
                     /locus_tag="WJL87_001061"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ7799492.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     MQHEADIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGNQVALQGNFDPSALFGTPESIRAEVARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            5202..6581
                     /gene="radA"
                     /locus_tag="WJL87_001062"
     CDS             5202..6581
                     /gene="radA"
                     /locus_tag="WJL87_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ7799493.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSSVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQTA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(6856..7335)
                     /locus_tag="WJL87_001063"
     CDS             complement(6856..7335)
                     /locus_tag="WJL87_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ7799494.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPQTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAAHRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(7467..7826)
                     /locus_tag="WJL87_001064"
     CDS             complement(7467..7826)
                     /locus_tag="WJL87_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ7799495.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPLSAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAKAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            complement(7865..11479)
                     /gene="recB"
                     /locus_tag="WJL87_001065"
     CDS             complement(7865..11479)
                     /gene="recB"
                     /locus_tag="WJL87_001065"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ7799496.1"
                     /translation="MSAPIQAFDPLTVPIAGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAKLGDDTLSDGIAAYCAE
                     HHKGDTFLPALLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLVPAQDFWRERVSNDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQTDLPQARDAALASWQNICGNLAELKETFWRIHPLLNGNSYRKNSFAELF
                     DALEQKALSPDLPFLEAKLHERLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFRRD
                     LDALEEAEEATIIRLQLDLIGYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPERPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAAECRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNSGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQ
                     DSRETVRQTYDREKDGIAMLKRNWRRLTDNAPAGTDFAFTEDAPPPAAYRGNAGQTAE
                     FAANSIPERRFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYCLSDIPPECRCPEMGFTLHTEDFGLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPDGNICVIDYKSNHLGADASAYTQQAMDEAVAHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK
                     "
BASE COUNT         2520 a         3049 c         3419 g         2545 t
ORIGIN      
        1 tgccgcccta tgtgttgccc taatttggaa ggggttacac ccttttcaaa taaaatctga
       61 tgctgctgcc acgaaggacg gatgtccaag tggcggggtt ttaaccatta aggaaatacg
      121 atgaaaaaaa cgttcctttc tgccgtattg cttctgtcag ctgcgaccca aaccgtgtgg
      181 gcagatacgg tgttttcctg taaaacggac aacaacaaat atatagaagt tcaaaaaatt
      241 aaccgcaatc tttacgaata ttcgttcggc agtgcggcaa aaaaagaaat tgccatacgc
      301 aacagcaaag ctgatctgtt ggggcgttcc gacaggtggc aaggctgggg cagaggccgt
      361 tgggcgacga tgaaattcca aaacggcgaa tttatgtaca ccgtagatgc aggcttcgat
      421 tccgtgaccc atgcagtagg cagcggtgtc cttgtggagc gtaggggcaa ggaaatcgca
      481 cggttcgact gtacgtcgaa aaccgcgcag gcgaatttcg acgatgacga ttttgcctgg
      541 taatcggggc ggataaggcg atagaaacag tgaaacccat catgctgatt gtccgaccgt
      601 cgggcagggc gaaggacgat gtggaaacct gccgtcgtgc cgaatggcag gcggaagtgt
      661 tgagtccgat tgaaatcgaa accgacaaag caagcctgaa acggctgccg gaaatgtatg
      721 cccgtgcgga tgccgtgttt tgggtcagtc cgaccgccgt tgaaaccgcc gtcccgtacc
      781 ttaacctttc agacggcata aagatgcaca tttccgtagg gcagggcagc cgccgtgcat
      841 tggaacgctg tttgggcaga acggtcatcg cgcctgatga cggcaacgac agcgaggcgg
      901 ttttgcgcct gccggtttgg aacagtctgc ccgaaggtgc gcgcgtattg tttgtgcgcg
      961 gacacggcgg gcgggatttt ctgatgaatg ccttgcagga gaaaggtttt cggacggagg
     1021 tggcagaagt ctatttcaga cggcataaac ctttgaactt tcaaaatttc caaaccgaaa
     1081 atattgccgc cgcctatatt acgtccgcag aactcgtgca gtcgctgttt gcacaggttc
     1141 cgccgcaatt ttcccgattc ttcaaatcct tgctatactt tacccatcat ccgcgcattg
     1201 cggaggcatt gaagcgcgaa ggcgtgtgtt cggtcgaaac cgtccctacg ctggaagccg
     1261 cgctttccca ttcttccatt tccgtttcag acggcatggt ctttcccgga acctcaaatt
     1321 aataaggagc aaaacggtgg gcgaacctga aaacaaatca tccgaacccg tacgcgagat
     1381 acaggcatca aaagaaatgc cgtctgaaac ctcttcccca cgcaaagaaa acgaaacaga
     1441 agtacacatt cctgccgctc cttttatcgt caaacagtcc ggcagcaacg ctttggcagt
     1501 ctgcgccctg gtattggcgg cattgggttt gggtacaagt ggttttttgt ttgtccaggg
     1561 acagaatgtc ttgaaaaacc aagagctggc attcaaccaa aaaatcgaca aagccgcctt
     1621 gggcgagtcg gaaaacgccg ccctgttgaa agacaacctc aaccggcaag ccgccataca
     1681 atcagagctc gaccgtttgg acggaaacgt caaagcaaac ggcgaacaaa tcttggaaat
     1741 gcaaaaatcc tatcgcgagt tgaccaaagg acgcgccgat tggctggtgg acgaaaccga
     1801 gaccatactc aatctggcgg cgcaacagct ggtgttgacc ggcaatatcc aaacggcagt
     1861 cggcgtattg gagcatatcg acagccgcct gtcccgtttc gatcaggcag agcttctgcc
     1921 gatcaaacag gcagtcagca gcgacttggc ggaactgaaa aaccgtccct atgtcgatat
     1981 ttccggcacg gcattgcgcc tcgacaggct ggaaaccgcc gtatccggac tgccgctgat
     2041 gctcgacggc gtattgaagc cgggcgtaca ggtgaagaac gaagccgctt ctgcttcatg
     2101 gtggcagaac gtatgggaaa aatccctcgg cacattgaag gggctggtcg aaatccgccg
     2161 tttggaaaac aacgatgcca tgctgatttc tcccgaacag gcatattttg tgcgtgaaaa
     2221 cctgcgcctc cgccttttgg atgcgcggac tgcattaatg cagcgcaaca gcgaagtcta
     2281 tcagggcgat ttgaacaatg ccgaagccgc cgtcagacag tatttcgatg ccaagtctcc
     2341 cgccacgcag tcgtggctga aagaactggc ggaattgaag gcgttggatg tgcgcatgac
     2401 ggcggatgac ggtttgaaaa acagcctaaa tgccgtccgc gcctatcgcg acggtacgcg
     2461 catgacggcg gcggaaaatc aagaagcaga acaggcggct tccgaaccgg caaacgaaaa
     2521 cacagcttcc gaaccggctg ccgcatcgga tgtgaagacc atagaagcac cgtccctgcc
     2581 ttcggaacgc aaaccggaac agcctgcaaa aaaacagccc gtaccggaaa aggcagggcg
     2641 ttcgccgtcc gctaaaggag aacgcgcatg aaaacggtag tctggattgt cgtcctgttt
     2701 gccgccgccg tcgggctggc attggcttcg ggcatttaca ccggcgacgt gtatatcgta
     2761 ctcggacaga ccatgctcag aatcaacctg cacgcctttg tgttaggttc gctgattgcc
     2821 gtcgtggtgt ggtatttcct gtttaaattc attatcggcg tattcaatat ccccgaaaag
     2881 atgcagcgtt tcggttcggc gcgtaaaggc cgcaaggccg cgcttgcctt gaacaaggcg
     2941 ggtttggcgt attttgaagg gcgttttgaa aaggcggaac tagaagcctc acgcgtgttg
     3001 gtcaacaaag aggccggaga caaccggact ttggcattga tgttgggcgc acatgccgcc
     3061 gggcagatgg aaaacatcga gctgcgcgac cgttatcttg cggaaatcgc caaactgccg
     3121 gaaaagcagc agctttcccg ttatcttttg ttggcggaat cggcgttgaa ccggcgcgat
     3181 tacgaagcgg cggaagccaa tcttcatgcg gcggcgaaga tgaatgccaa ccttacgcgc
     3241 ctcgtgcgtc tgcaacttcg ttacgctttc gacaggggcg acgcgttgca ggttctggca
     3301 aaaaccgaaa aactttccaa ggcgggcgcg ttgggcaaat cggaaatgga acggtatcaa
     3361 aattgggcat atcgccgcca gctggcggat gctgccgatg ccgccgcttt gaaaacctgc
     3421 ctgaagcgga ttcccgacag cctcaaaaac ggggaattga gcgtatcggt tgcggaaaag
     3481 tacgaacgtt tgggactgta tgccgatgcg gtcaaatggg tcaaacagca ttatccgcac
     3541 aaccgccgcc ccgagctttt ggaagccttt gtcgaaagcg tgcgcttttt gggcgaacgc
     3601 gaacagcaga aagccatcga ttttgccgat gcttggctga aagaacagcc cgataacgcg
     3661 cttctgctga tgtatctcgg tcggctcgcc tacggccgca aactttgggg caaggcaaaa
     3721 ggctaccttg aagcgagcat tgcattaaag ccgagtattt ccgcgcgttt ggttctggca
     3781 aaggtttttg acgaaaccgg agaaccgcag aaggcggagg cgcagcgcaa cttggttttg
     3841 gcaagcgttg ccgaggaaaa ccgaccttcc gccgaaaccc attgaaacct tcagacggca
     3901 tcgttgaaat gccgtctgaa aaccatttca cttgacaaac agactgacag aaaacccgta
     3961 tgactctttt aaaaaacgat accttcctcc gcgccctgct caaacagccc gtcgaataca
     4021 cgccgatttg gatgatgcgc caggcggggc gttatctgcc cgaatacaaa gccacacgcg
     4081 cgaaagcggg cagcttcctc gatttgtgca aaaacaccga attggcgacc gaagttacca
     4141 tccaaccttt ggaacgcttc gatttggacg cggcgatttt gttttccgac atcctgaccg
     4201 tccccgacgc aatgggcttg ggactgtatt ttgccgaagg cgaaggcccg aaattcaaac
     4261 gcgccatgca acacgaggcc gacatcgcca agctgcacgt tcccgatatg gaaaaactgc
     4321 aatacgtttt cgacgcggta acttccatcc gtaaagcatt ggacggccgc gtaccgctta
     4381 tcggcttttc cggcagcccg ttcacactcg cctgttatat ggtcgaaggc ggcggcagca
     4441 aagaattccg caccatcaaa accatgatgt actcgcgccc cgatttgctg cacaaaatcc
     4501 tcgataccaa cgcccaagcc gttaccgcct acctcaacgc ccaaatcgac gcgggcgcgc
     4561 aggcggtgca aattttcgac acttggggcg gcgtgttgag cgacgcggcg tttaaagaat
     4621 tcagcctcaa atacatccgc cagatcgtcg ccggactcaa acgcgaaagc gaaggccgcc
     4681 gcgtgcctgt tatcgtattt gccaaaggcg gcgggctgtg gctggaaagt atggcccaaa
     4741 tcggcgcaga cgcattgggc ttggactgga cgtgcaacat cggcgaagca cgccgccgcg
     4801 tcggaaacca agtcgccctg caaggtaact tcgacccgtc cgccctcttc ggtacgccgg
     4861 aatccatccg cgccgaagtc gcacgcatcc tagccgacta cggacacggc agcggccatg
     4921 tcttcaacct cggacacggc atcaaccaac atgccgaccc cgaacacgcc aaaatcttag
     4981 tcgacaccgt acacgagctg tcgcggcagt atcacggcgg gtaagccggc aggaaaccgc
     5041 ccgatatgcc gtctgaagcc gagggatgga aaaataaggc aatgcggcaa tatccgccgt
     5101 gtatggatag tacatgacgg cggcggtgtt gccgcattgg cgcaatccca accgtcccta
     5161 ttttcagacg gcatttttgt tttcagatgc agggaaaacc gatggcaaaa acgcttaaaa
     5221 ccctttacca atgcaccgaa tgcggcggca cttccccgaa atggcagggc aaatgcccgc
     5281 attgcggcga gtggaacaca cttcaggaaa gcttggccgc gcccgagccg aaaaacgccc
     5341 gcttccaatc ttgggcggcg gatacctcga ccgtccaatc cctctcctct gttaccgcga
     5401 ccgaagtgcc gcgcaatccg accggtatgg gcgaactcga ccgcgtattg ggcggcggtt
     5461 tggtcgatgg tgcggtcatc ctgctcggcg gcgatcccgg catcggcaaa tccacgctgc
     5521 tgttgcaaac cattgccaaa atggcgcaaa gccgcaaagt gctgtacgtt tccggcgagg
     5581 aatctgccca acaggtcgcc ctgcgcgcgc aacgtttgga actgcccaca gaaggtgtga
     5641 acctgcttgc cgaaatccgc atggaggcga ttcagacggc cttgaaacag catcagcccg
     5701 aggtcgtcgt catcgactcc atccaaacca tgtattccga ccaaatcact tccgccccag
     5761 gctccgtgtc gcaggtgcgc gagtgtgccg cccaactgac gcgcatggcg aaacagatgg
     5821 gcatcgccat gatactggtc ggacacgtga ccaaagacgg cgcgattgcc ggcccgcgcg
     5881 tgctggaaca catggttgat accgtgctgt atttcgaggg cgaccaacat tccaactacc
     5941 gcatgatacg cgccatcaaa aaccgcttcg gcgcggcaaa cgaactgggc gtgttcgcga
     6001 tgacggaaaa cggtttgaaa ggtgtgtcca acccgtccgc catcttcctc gccagctacc
     6061 gcgacgatac gcccggctcg tgcgttttgg ttacacagga aggcagccgc ccgcttttgg
     6121 tcgaaattca ggcattggtc gatgacgcgc acggcttcac gcccaaacgc ctcaccgtcg
     6181 gactggaaca aaaccgtctt gcgatgctgc ttgccgtgtt aaaccgccac ggcggcatcg
     6241 cctgtttcga tcaggatgtg ttcctcaacg ccgtcggcgg cgtgaaaatc ggcgaaccgg
     6301 cggcggattt ggcggtcatc ctcgcgatgc tttccagctt ccgcaaccgc cctatgcctg
     6361 aaaaaaccgt ggttttcggc gaaatcggct taagcggcga agtccgcccc gtcgcacgcg
     6421 ggcaagagcg gctcaaagaa gcggaaaaac tcggcttcaa acgcgccatc gttcccaaag
     6481 ccaatatgcc gcgcaacgcc aaagagtttc cgaacctgaa aatctacggc gtttcgagtt
     6541 tgcaggaagc catcgatatt tgccgtgaca gcagggaata aacggaaatg ccgtctgaaa
     6601 tcaggtttca gacggcattt ggtttgtggc ggattgaaac aagaaggcat accggcgaca
     6661 gataagattt gcggcaaagt tgcctgtgat atggcaaaac gcacacgccc gtcatccccg
     6721 caagggcggg aatccggaat cgtccgtttc ggcaatgatt gaaaatcacg gtaacccaac
     6781 cgcttggatt cccgacttcg tgggaatgag gggcgtgtgt gtttgatttc catccgccat
     6841 atgtcggcga cgggcttatt cgcctacggt tttttgtatc agtttttcgg cgtttgccaa
     6901 agtgtttgcc acttcgtcaa aaccgatgcg gctgccggcg atgagggcgc gcgtatcggt
     6961 ataggcggcg cgtactttgc cgtctgtttc ggtaacgagg acgcgcaggg gcagttgcag
     7021 ggcgaaggcg gggtctttga ccatcagcgg cgtgccggct ttgggcgtgc cgaagacgat
     7081 gacttttgcc ggctgcatcg ttaagccgtt tcggtgggcg gcttcctgat ggtcgatgac
     7141 ggcaaaaatg tccatccctt tgctttttat ggcggtttca aggcggctga cggtttcgtc
     7201 aaaactgtat tttgaggtga gggtatgcgt ggtcatagcg gtttcgtttt gggtttgcgg
     7261 ttcgctggca ggatgtgccg aagcggttga aatgcagagt gcggatgcgg caatcagggg
     7321 gagtatgtgt ttcatcgtat ttcctttttc cttttcggtt tgtttatcag gcttcggacg
     7381 gggcggcacg cgccgcgccc gatgttgcgc cgcgtgctgg ggtgtcgtat gccgtctgaa
     7441 agcctgtcct ttcagacggc attgcgtcat ttcatccctt ttttgagcag gtcttcataa
     7501 ccgccgtgat tggcaacatt tgtataacct gcttttttca gctcctgaag ggcggctttg
     7561 gcacgccgtc cgctgcggca gtagaggttg accggcgtgt ctttgtcggg cgcggcttcg
     7621 tgtatgcggc ggacgatttg gtcgacgggg atgttgaccg cgttgtgcaa atgaccttcg
     7681 ctaaattcct gttcggaacg gacatcgatc caaacggccg gatgttgcgc ggtttgggcg
     7741 gcggataggg gtttttgcgg ggctgcctgc gcggcaaagg cggctgaggc aatgagtgcg
     7801 gcggtaatca ggtgtttgat attcataggg ttctcctgcg gttgtcgtct taagggacgg
     7861 gaagttattt tatctgttcc aaagcggcgg catctatgtc ccaacgccaa acgccgccgc
     7921 ctttgccatc caatccgcgc aaaaacaggt agcggacgga aacggcggcg ggcggttgtc
     7981 cgcgcagttt gaagtagcgt gcggcggcaa cggcgtaaat cagcgcctga aggtaatagt
     8041 ggtggtgcgc gacggcttcg tccatcgcct gttgcgtgta ggcggatgcg tccgcgccga
     8101 gatggtttga tttgtagtcg atgacgcaga tattgccgtc ggggtcttgg cagaccatat
     8161 cgataaagcc gtttaaaaag ccgttgacgg tgtggaagtc gagcgtttcg gcggcggcac
     8221 ggcagacttc gggcaatcgg atatcgtcgc gggcaaacca gtcgcgcagc cgtttgaggc
     8281 cgaagtcttc ggtgtggagg gtaaagccca tttcgggaca gcggcactcg ggcgggatgt
     8341 cggacaggca gtatgccccc gtcagcggcg ttttgcggca ggcttccgcc atttcggcaa
     8401 cggcaggcag ccatatttct tcaaatccgt attttttcag cttgtcggca atgagagttt
     8461 cctgtccggc ggctgcttgt ccgaatttga aatcttcaag aatttcgtgc aggcacagcc
     8521 ccgcctgcgt gcctttcgga aaatcgtgta tcgatatgcc gtctgaagcc gtcggcgttt
     8581 cagacggcat cgccggcacc gaggtttcgg cggcatccaa ggacgggcag gcatcttctt
     8641 cgccgccgtc gggcgtttgg gtatggcggc ttaaggcggt aaagctggtg tggcggacaa
     8701 atcggaatct gcgttcggga atgctgtttg cggcaaattc ggcggtttga ccggcgttgc
     8761 cgcgataggc ggcagggggc ggcgcatctt ctgtgaaggc gaaatccgtg ccggccgggg
     8821 cattgtccgt caaacgccgc cagttgcgtt tgagcatcgc gatgccgtct ttttcccggt
     8881 cataggtttg gcggacggtt tcgcggctgt cttgcggcaa gccttcaatc aggtaggcga
     8941 gggggttgtc ggcggtatcg gaagaatagg cggcgtagat gttgagctgt tcctcggcac
     9001 gcgtcagcgc gacataaagc aggcgcaggc gttccgccat ttcctcatcg gcgtattgct
     9061 tctgttcgtc ttccgacagt tgcgcctttg ccaacagttc ggttcgattt gcgctttggt
     9121 ggaggatttg ccagtcggac ggcccggtat cttgcgcgtc ccacgcaaac gggcagtaca
     9181 ccagcggata ctgcaaacct ttcgaggcgt gcatggtaac gattttgacc aaatcttcgt
     9241 cgctttccag acggatggcg cggttgtcgc cgctgttgtt gccggccagg ctgatttggt
     9301 cgcgcaacca tttgtgcagc gcggcggggt tgcggttttg cgcgtcttcg gcggcaagca
     9361 gttcgagcag ttggaaataa ttggtcaggc tgcgcccgtt gttccggctt aagaggcgcg
     9421 tttcgatgcc gtgtgtttgg gaaaattgct gcatggcggc gaaaatgccg tattgattcc
     9481 agttgtcgag cgcggttcgg gcagattccg cccaatgcaa aatctcgctt tcgttttggt
     9541 tgaagtcgtg caattgctgc gcgtcataac cgaatatgcc gcttgtcagg acaaagcgca
     9601 gcgttccggc gcggcgcggt tcgagccaga agccgatgag tgcggaaagg gcggcggctt
     9661 cgggcgaggc gaacacggat tcgcgcgaaa gcaggacgct ttgcacctgc cgttttttca
     9721 gggcagcgga aaccatcacc gcctcgttgt gcgtgcgtac cagcacggca atatcgcccg
     9781 actgcaacgg gcagcctttg aaattcagac ggcattcggc ggcttcgttg agcgcgtggg
     9841 cgatttcgtc ggcgcaatag tcggcggcac ggcggcgcaa aacgtctttg ttggcttttt
     9901 cattgtcgtt ttcgtgcagc caacgaacct gtacggcagg acgttcgggg gacaacctgc
     9961 tttcggcacg cgccgcaccg acttccgaat agccgatgtt ttccaaaacg aacgggcgtt
    10021 ctttgaggcg gaacagcgcg cctatgctgc cgataagcgc ggcgtggctg cggtagttgg
    10081 tggcgagcgt gtagcggtgc cgcgcgtctt ccgccgcctg aaggtaggcg taaatgtccg
    10141 ctccgcgaaa gctgtaaatc gcctgtttgg gatcgccgac gaggaacagc ggtcggtttt
    10201 gggcgatgaa gattttttgg aagatttcgt attgcagcgg gtcggtgtct tggaactcgt
    10261 cgatcagcgc ggtttcccag ttttcggcaa cggcgcgggc gagggtttcg gcgtgcggat
    10321 tgtcggtcag cgcggtgtgg acatcgagca gcaggtcgtc gaaaccgcgt tcgcggcgcg
    10381 attttttcat ctcggcaagg ctgcggttga ggtatccgat taaatccagt tgcagccgga
    10441 ttattgttgc ttcttccgct tcttcgagtg cgtccaaatc acgcctgaag tctgccagtt
    10501 tctgcaattc ggcaaatact gccgcatcgg gcgttttgcc ttttttcagt ccggcttcga
    10561 gtttgtcgga cgcaagtttc aagagccgtt cgtgcagctt tgcctccaaa aagggcaaat
    10621 ccggcgacag ggctttttgt tccaatgcgt caaacagttc ggcaaaactg tttttccgat
    10681 atgaattgcc gtttaaaagg ggatggatgc gccaaaaagt ttcttttaat tcagccagat
    10741 ttccgcaaat gttttgccag cttgccaaag cggcatcccg ggcttgcggc aaatcggttt
    10801 gcggacggcg gaaattcagg tacgggcggg acaggtaggc gcggatttgg gcaaggacgg
    10861 tttgcggcac agctttgcgt ttaaacgcca atgcggcaag caccggatca ttgctgacgc
    10921 gttcccgcca aaaatcttgc gccgggacaa gcaggcggtc gccgtcttct tcggtcagtt
    10981 cgacatcgaa cggtgcttgg cacaaaaacg cgtagtcgcg caggatgcgc tggcagaagc
    11041 cgtggatggt atagatggcg gcgttgtcga attgcccgat ggcggctttg aggcggacaa
    11101 tcagacgcgt ccggccctct ttttgcaaag cctgttttaa gagtgcgggc aggaaggtgt
    11161 cgcctttgtg gtgttcggcg cagtaggcgg cgatgccgtc tgaaagcgtg tcgtctccaa
    11221 gtttggcaat ttctttactt tctaaaacct gcaacacatc gtccaaacgt gcgcgcaggc
    11281 gtgttttcaa ctcggcggtg gcggctttgg tgaaggtaac gaccaatacg cgctcgacgt
    11341 ttttttgttc caatacgatc aggcgcgtaa acagggcggc aatgccgtag gtttttccgg
    11401 tgccggcaga ggcctcaatc aggttggtgc cggcaatggg gacggtcagc gggtcaaatg
    11461 cttggatggg tgcagacata gtgcgcgctc ggaaaacggt tggacggtat agtggattaa
    11521 caaaaatcag gat
//
LOCUS       DAWKST010000059        11305 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.059, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000059 DAWKST010000000
VERSION     DAWKST010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11305
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.059"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            42..455
                     /locus_tag="WJL87_001066"
     CDS             42..455
                     /locus_tag="WJL87_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ7799497.1"
                     /translation="MMPAYAYAALAFSAFTSATLLPGTSEAAFALFVRRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLPFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            501..1130
                     /gene="nth"
                     /locus_tag="WJL87_001067"
     CDS             501..1130
                     /gene="nth"
                     /locus_tag="WJL87_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ7799498.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            1268..2767
                     /locus_tag="WJL87_001068"
     CDS             1268..2767
                     /locus_tag="WJL87_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ7799499.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSSNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQEGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLIMRRGNTLFIALNLQ"
     gene            2967..4487
                     /locus_tag="WJL87_001069"
     CDS             2967..4487
                     /locus_tag="WJL87_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ7799500.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAIITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAVSDGSRGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVDPSLIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            4544..5629
                     /gene="nagZ"
                     /locus_tag="WJL87_001070"
     CDS             4544..5629
                     /gene="nagZ"
                     /locus_tag="WJL87_001070"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ7799501.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAVGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMSTLGEIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHHNPEAVAHLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDRRSLSELEAADLAPFHIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGVGGLKERAR
                     ISFEAGCDIVLVCNRPDLVDELREDFRIPDNPALAQRWQYMANTLGSAAAQAVMQTAD
                     FQEAQAFVAGLASPQDTAGGVKVGEAF"
     gene            5857..5933
                     /locus_tag="WJL87_001071"
     tRNA            5857..5933
                     /locus_tag="WJL87_001071"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5891..5893,aa:Pro,seq:cgg)
     gene            complement(6269..6907)
                     /locus_tag="WJL87_001072"
     CDS             complement(6269..6907)
                     /locus_tag="WJL87_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ7799502.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGIPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDMLKAEGYFL
                     CIETNGLNPAPPQIDYVATSPKACYAAKYEKSCIETADEVRIVADGDVLAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDASVHWQLSVQTHKWAGIE"
     gene            complement(6904..7275)
                     /locus_tag="WJL87_001073"
     CDS             complement(6904..7275)
                     /locus_tag="WJL87_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ7799503.1"
                     /translation="MKLLSTLLVLLVTAEHFYIAWLEMTQIPSEKAAEIFKLPYEFME
                     QKRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            complement(7544..7966)
                     /gene="queD"
                     /locus_tag="WJL87_001074"
     CDS             complement(7544..7966)
                     /gene="queD"
                     /locus_tag="WJL87_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ7799504.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGIIKGG
                     AKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNSRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            complement(7973..8488)
                     /locus_tag="WJL87_001075"
     CDS             complement(7973..8488)
                     /locus_tag="WJL87_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ7799505.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSETPKTEMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDHVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGKP
                     AEIGFQGYLPI"
     gene            complement(8516..9175)
                     /gene="queC"
                     /locus_tag="WJL87_001076"
     CDS             complement(8516..9175)
                     /gene="queC"
                     /locus_tag="WJL87_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ7799506.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQIYGRENVQAITFQYGQRHA
                     VELERARWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGVLDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            9280..9705
                     /locus_tag="WJL87_001077"
     CDS             9280..9705
                     /locus_tag="WJL87_001077"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799507.1"
                     /translation="MPSEHAAYADLMASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSAGGIAGATVLEIVEDEDFEGDLGSWADSVKM
                     VRSSDGITVVPMPSETAAVQAFVPDGKIGYNIGHQCPTY"
     gene            9720..10946
                     /locus_tag="WJL87_001078"
     CDS             9720..10946
                     /locus_tag="WJL87_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ7799508.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWR
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKKGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMTPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(10967..11248)
                     /locus_tag="WJL87_001079"
     CDS             complement(10967..11248)
                     /locus_tag="WJL87_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ7799509.1"
                     /translation="MIHEEICAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDAATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
BASE COUNT         2473 a         3147 c         3028 g         2657 t
ORIGIN      
        1 acaatccgtc gccgattttt gcctgtttga gactgtttgc aatgatgccc gcctacgcct
       61 acgccgcgct cgccttttcc gccttcactt ccgccaccct cctgcccggc acatccgaag
      121 ccgcctttgc cctgttcgtc cgccgctttc ccgaacacgc atacggcgcg ttgctgtgcg
      181 ccggccttgc caacggattg ggcagtatgg tttcctattg gatgggacgt ttgctgcctt
      241 cccgaaaaat gccgtctgaa aaaacactga atctgatgcg gcgtttcggt atttggctgc
      301 ttccgtttgc ctggctgccc gtcgtcggcg acgcactgcc gcttgccgcc ggctggctgc
      361 ggttgaatcc gtggacaagc gggctgatgc tggttatcgg caaaacggcg cgttacgcct
      421 tcattctgtg gggaatgcaa tattacgccg cctgaaccgt gcgttataat gccgaccgca
      481 caaaccgact gcacaaaaaa atgaacagac acatccgcca agaaattttc gaacgcttcc
      541 gcgccgccaa cccccatccg accaccgagc tgaatttcaa ctcccctttc gagcttttaa
      601 ttgccgttct gctttcggcg caggcgaccg atgtcggcgt aaacaaggcg acggcgaagc
      661 tgtttccggt tgccgatacg ccgcaggcga tgctggattt gggtttggac ggcgtgatgg
      721 aatacacgaa aaccatcggg ctgtataaaa ccaagtccaa gcacatcatg caaacctgcc
      781 gcatcctgct ggaaaaatac aacggcgaag tgccggaaga ccgcgaggct ttggaatcgt
      841 tgcctggcgt ggggcgcaaa acggcaaacg tggtattgaa cacagcgttc ggacaccccg
      901 ttatggcggt cgatacgcat attttccgcg tgtccaaccg aaccaaaatc gcccccggga
      961 aagatgtgcg cgaagtcgaa gacaaactga tgcgcttcat tcctaaagaa tttctgatgg
     1021 acgcgcacca ctggctgatt ttgcacggac gctacacctg caaggcactc aaaccgcaat
     1081 gccaaacctg catcatcaac gatttgtgcg aatatcccgc caaagcctga ccgccgccgc
     1141 ccttcaaaat gaaacgcttg aaaattcgga cggacgttgt agaatttcaa cctatgccgt
     1201 ctgaagccga caaaatgcgc ttcagacggc attaaagccg gtaccggcat ccgacaaagg
     1261 acaaaacgtg ttcaaaaaat accaatacct cgctttggca gcactgtgtg ccgcctcgct
     1321 ggcaggctgc gacaaagccg gcagcttttt cggtgcggac aaaaaagaag catctttcgt
     1381 agaacgcatc gaacacacca aagacgacgg cagcgtcagt atgctgctgc ccgactttgt
     1441 ccaactggtt caaagcgaag gcccggcagt cgtcaatatt caggcagccc ccgccccgcg
     1501 cacccaaaac ggcagcagca atgccgaaac cgattccgac ccgcttgccg acagcgaccc
     1561 gttctacgaa tttttcaaac gcctcgtccc gaacatgccc gaaatccccc aagaagaagc
     1621 agatgacggc ggattgaact tcggttcggg cttcatcatc agcaaagacg gctatattct
     1681 gaccaatacg cacgtcgtta ccggcatggg cagtatcaaa gtcctgctca acgacaagcg
     1741 cgaatatacc gccaaactca tcggttcgga tgtccaatcc gatgtcgccc ttctgaaaat
     1801 cgacgcaacg gaagagctgc ccgtcgtcaa aatcggcaat cccaaagatt tgaaaccagg
     1861 cgaatgggtc gctgccatcg gcgcgccctt cggcttcgac aacagcgtga ccgccggcat
     1921 cgtgtccgcc aaaggcagaa gcctgcccaa cgaaagctac acacctttca tccaaaccga
     1981 cgttgccatc aatccgggca actccggcgg cccgctgttc aacttaaaag gacaggtcgt
     2041 cggcatcaac tcgcaaatat acagccgcag cggcggattc atgggcattt ccttcgccat
     2101 cccgattgac gttgccatga atgtcgccga acagctgaaa aacaccggca aagtccaacg
     2161 cggacaactg ggcgtgatta ttcaagaagt atcctacggt ttggcacaat cgttcggttt
     2221 ggacaaagcc ggcggcgcac tgattgccaa aatcctgccc ggcagccccg cagaacgtgc
     2281 cggcctgcag gagggcgaca tcgtcctcag cctcgacggc ggagaaatac gttcttccgg
     2341 cgaccttccc gttatggtcg gcgccattac gccgggaaaa gaagtcagcc tcggcgtatg
     2401 gcgcaaaggc gaagaaatca caatcaaagt caagctgggc aacgccgccg agcatatcgg
     2461 cgcatcatcc aaaacagatg aagcccccta caccgaacag caatccggta cgttctcggt
     2521 cgaatccgca ggcattaccc ttcagacaca taccgacagc agcggcggac acctcgtcgt
     2581 cgtacgggtt tccgacgcgg cagaacgcgc aggcttgagg cgcggcgacg aaattcttgc
     2641 cgtcgggcaa gtccccgtca atgacgaagc cggtttccgc aaagctatgg acaaggcagg
     2701 caaaaacgtc cccctgctga tcatgcgccg tggcaacacg ctgtttatcg cattaaacct
     2761 gcaataacac acataattta accaaaatat tttttaaatg ataaaatgcc cgtcaatacc
     2821 gattaacggg cattttaaaa aatcagttaa ttgatttcaa ataagtattt tccggaacag
     2881 aaaatacttt cgcccatttt gccaacaaaa tccgaaccgt tgcggacaga aaccgcccca
     2941 tccggacgct taacaccccc ataaccatgc agctgatcga ctattcacat tcatttttct
     3001 cggttgtgcc accctttttg gcactggcac ttgccatcat tacccgccgc gtactgctgt
     3061 ctttaggcat cggtattctg gtcggcgttg cctttttggt cggcggcaac cccgtcgacg
     3121 gtctgacaca cctgaaagac atggtcgtcg gcttggcttg gtcagacggc gattggtcgc
     3181 tgggcaaacc aaaaatcctg gttttcctga tacttttggg tatttttact tccctgctga
     3241 cctactccgg cagcaatcag gcgtttgccg actgggcaaa acggcacatt aaaaaccggc
     3301 gcggcgcgaa aatgctgacc gcctgcctcg tgttcgtaac ctttatcgac gactatttcc
     3361 acagcctcgc cgtcggtgcg attgcccgcc ccgttaccga caagtttaaa gtttcccgcg
     3421 ccaaactcgc ctacatcctc gactccactg ccgcgcctat gtgcgtgctg atgcctgttt
     3481 caagttgggg cgcgtcgatt atcgccacgc ttgccggatt gctcgttacc tacaaaatca
     3541 ccgaatacac gccgatgggg acgtttgtcg ccatgagcct gatgaactat tacgcgctgt
     3601 ttgccctgat tatggtgttc gtcgtcgcat ggttttcctt cgacatcggc tcgatggcgc
     3661 gtttcgaaca agccgcgttg aacgaagccc acgatgaaac tgccgtttca gacggcagcc
     3721 ggggcagggt ttacgcattg attattcccg ttttggcctt aatcgcctca acggtttccg
     3781 ccatgatcta caccggcgcg caggcaagcg aaaccttcag cattttgggg gcatttgaaa
     3841 acacggacgt aaacacttcg ctggtattcg gcggcacttg cggcgtcctt gccgtcgttc
     3901 tctgcacgct cggcacgatt aaaaccgccg actatcccaa agccgtttgg cagggtgcga
     3961 aatctatgtt cggcgcaatc gccattttaa tcctcgcttg gctcatcagt acggttgtcg
     4021 gcgaaatgca caccggcgat tacctctcca cactggttgc gggcaacatc catcccggct
     4081 tcctgcccgt catcctcttc ctgctcgcca gcgtgatggc gtttgccaca ggcacaagct
     4141 gggggacgtt cggcattatg ctgccgattg ccgccgccat ggcggtcaaa gtcgatccct
     4201 cactgattat cccgtgtatg tccgcagtaa tggcgggggc ggtatgcggc gaccactgtt
     4261 cgcccatctc cgacacgacc atcctgtcgt ccacaggcgc gcgctgcaac cacatcgacc
     4321 acgttacctc gcaactgcct tacgccttaa ccgttgccgc cgccgccgca tcgggctacc
     4381 tcgcattggg tctgacaaaa tccgcgctgt tgggctttgg cacgacaggc attgtattgg
     4441 cggtgctgat ttttctgttg aaagataaaa aacgcgccaa cgcctgaccc ctttccctgt
     4501 tcagacggca tatgccgtct gaagcttttt ggagcaaacc gcaatgaccg tcccccatat
     4561 tccgcgcggc cccgtcatgg cagacattgc cgccttccgc ctgaccgaag aggaaaagca
     4621 acgcctgctc gatcccgccg taggcggcat catcctcttc cgccgcaact tccaaaacat
     4681 cgaacaactc aaaacactca ccgccgaaat caaagccctg cgcacacccg aactcattat
     4741 cgccgtcgat cacgaaggcg gcagggtgca acgtttcatc gaaggcttca cccgcctgcc
     4801 cgcaatgagt acgctcggcg agatttggga caaagacggc gcgtccgccg ccgaaaccgc
     4861 cgccggacaa gtcggccggg ttttggcaac cgagctttcc gcctgcggca tcgatttgtc
     4921 cttcacgccc gtcttagatt tggactgggg aaactgcccc gtcatcggca accgcagttt
     4981 ccaccacaat cccgaagcgg tcgcccacct cgccctcgcc cttcaaaaag gtttggcaaa
     5041 aggcggtatg aaatcctgcg gcaaacattt tcccggacac ggatttgtcg aaggagacag
     5101 ccatctggtc ttgccggaag acaggcgcag cctgtccgaa ctcgaagccg ccgacctcgc
     5161 ccccttccac attatgagcc gcgaaggtat ggcggcggtt atgcccgccc acgtcgttta
     5221 tccacaagtg gacacaaagc ccgcagggtt ttccgaaatc tggctcaaac aaattttgcg
     5281 ccgcgatatc ggattcaaag gcgtgatttt ctcggacgat ttgactatgg agggcgcgtg
     5341 cggtgtcggc ggcctcaaag aacgcgcccg catttccttt gaggcaggtt gcgacatcgt
     5401 cctcgtctgc aaccgtcccg accttgtgga cgaactgcgc gaagatttcc gcattcctga
     5461 caatcccgct ttggcgcaac gttggcaata tatggccaac acgctcggca gtgccgccgc
     5521 gcaagccgtt atgcagacgg cggatttcca agaggcgcaa gcctttgttg ccggccttgc
     5581 ctcgccgcag gatacagcgg gcggcgtaaa agtcggcgaa gccttttaaa ccggctgcgt
     5641 ttcaacaatc tgccgatttc atcaaccgtt tgtcagggaa aggcaatgat tttttcggtc
     5701 aggtagaaag ttatcttatt gaattactta aagataatga tgattatcaa cactgtattc
     5761 aaacacactc ctttaaatcc gcccgacacg cggcaaaaag gcaaaacggt ataggcacgc
     5821 cgtatttttt gcgctataat cacgccttac ttaattcgga atgtagcgca gcccggtagc
     5881 gcacttcgtt cgggacgaag gggtcggagg ttcgaatcct ctcattccga ccatattcaa
     5941 accgcctcgg catggattgc tggggcggtt tttccctttt ggcgtcaaca gaaaaatgcc
     6001 gtctgaaaca gaatgctgtt tcagacggca tttcgttttc cctaaccctg ccatttgcgc
     6061 gcactggcac agagtatcgg caaggttcaa aagaccatcg gaatttagga agccgagctt
     6121 gtacccaacg gcagcatctt attcagtaag gctccgtcct gattgggcgg tggaaatgcc
     6181 ggcatttcca cgcagaagcg gcacactttc cccgtatgtc aaacatcggt aaaaccgtac
     6241 gccgtccctt tgagttacac tgcttaaact actctattcc cgcccatttg tgcgtctgca
     6301 cgctcaactg ccaatgcacg gatgcgtcgg ggcgactgtt taaaataccg atttggcgga
     6361 tggtgtcgta gatgttcatc gcaccgtctt gctcacaggg cgaaaggtag taatgatgcg
     6421 cgcggatttt gcgttccata ttttcacaga acgcaaggac atcgccgtcg gcaacaatcc
     6481 gcacttcgtc ggctgtttcg atacagcttt tttcgtattt ggcggcatag caggctttgg
     6541 ggctggtggc gacatagtcg atttgcggcg gcgcgggatt gagtccgttg gtttcgatac
     6601 agaggaaata accttccgcc ttgagcatgt ccagcagcat atcgagatgc ggctggatgg
     6661 tcggttcgcc gccggtgatg atgatgttgc gggcggcata ggttttcaga cagcctaaga
     6721 tgtcggacag gctcatcatg ccgaatgtca aataatcggt atcacaccag ccgcaggcga
     6781 ggttgcattt gcccaagcgg acgaaaacgg cgggtatgcc cgtgttccag ccttcgcctt
     6841 gcaggctttc aaagatttcg acgatgcggt attggggatt ttcgggggca acattgattt
     6901 ttttcatacc gccaacaccg ccgccgctgc caaaaatgcg ggcaaacctt gtttgacgag
     6961 tatgcctttg ttgccggaag agaacgcgcc ccacgcggcg gcaatcagga cgaagccgag
     7021 aaacagtacc gttgcgccgt acaccgcgtt atccggagcg gcaaaccgcg accacaccag
     7081 cccgatgccg agaaagccgt tatacagccc ttggttgccg aacagggtct gcacgcgctt
     7141 ttgttccata aattcataag gcagcttgaa tatttccgcc gctttttcgc tgggaatctg
     7201 cgtcatttca agccaggcga tgtagaaatg ttcggcggtg acaaggagga ctaagagggt
     7261 ggagagaagt ttcatgggta ttccttttaa tgggtgtatt tgatatggag ggtcgtctga
     7321 aaaaacgggg aatccgtaca ggagaaaaaa ccgttttacg gtaaattcaa ataaggacgc
     7381 tctaaaccgt cctttccgcg caggcgggaa tccaaagatt tggaattgct gcaattttta
     7441 aatattcctg aaaaaccaag gtctggattc ccgcctgcgc gggaatgacg cggttgggca
     7501 tgtcttattg gaatttacta tatcgatacg ttcaagatat tccctactcc ccttcatact
     7561 ccgcacacga tgtcggcgtt tcccacaatt tgacgcggca cacgttcagc cccgcgtttt
     7621 tcagacggtc gtacatttcg accgccatat tttcggcagt ggtgcggcag ggcaggcgca
     7681 gggttttcat gttccagcct tccaagagtg cggcgatttg agattcgcgg ctgttgccgc
     7741 cgtggtagat gaaggcgtgg tcgaaggggt cggtaatgtg ttgtttgaca atggctttca
     7801 agtcggtaaa gtccatcacc ataccgtctt tcgcgccgcc tttgataatg ccgtctgaaa
     7861 cggtgatttc gagtttgtag gtatgtccgt gcaagttttg gcatttgccg tcatgcccgt
     7921 cgagcatatg cgaggagtcg aaggtgaaga ttttggtgat tttcatgggt tctcaaatgg
     7981 gcaaatagcc ttgaaagccg atttcggcgg gtttgccgtg cggattcggg gtcaggcgga
     8041 tatggaaatt gccgatgcgg tcgagcgcaa gcaggttgtc cacgtctttt aagttgtctt
     8101 tatgttgctg aacatggtcg gtcaacaggg tttgcagggc tttttgtttg gcttcggcag
     8161 gcgtggcggc ggcgaacagc ccgaacgcgt gcgcctcggc aagctgcccc gcgcgataac
     8221 cgccgacatt ggcaaaatac aggcgcgggg cgtgttcaga cggcatttcc gttttgggcg
     8281 tttcggatac ggcgatgtcg taaccgtccg cccattcgac aacctgccag ccgtcgatgt
     8341 ggattttgtc cgcatcgccg aaccacgcgg ctttgagtgc ggggacggct tcacggtagt
     8401 cgtcgcacac ggcaaattgg atgtcgtgca cttcgatatt cgacctgccg gcattgccgc
     8461 cgaggtaaaa catatggagc ttgggcatat cgtattcctt atggtttgcg cgtgttcaga
     8521 cggccttttt actttccaga tattccgcca gcccgcgttc gcgcaagata cagctcgggc
     8581 attcgcggca gccgccgacg atgccgttat agcaggtgtg ggtttgctcg cggatatagt
     8641 ccagcacgcc catttcgtcc gccaacgccc acgtttgcgc cttggtcaga tacatcagcg
     8701 gcgtgtggat ttggaaatca tagtccatcg ccaaattaag ggtaacgttc atggatttga
     8761 cgaacacgtc gcggcagtca ggatagccgg agaagtcggt ttcgcacacg cccgcgatga
     8821 tgtgccgtat cccctgccct ttggcgtaaa tcgcggcata gagcaggaaa agcgcgttgc
     8881 ggccgtctac aaaggtattc ggaacgccgt tttcggcagt ttcgatggcg gcggtgtcgt
     8941 ccatcagggc attgtgcgta atctgccgca tcaggctcaa gtcgagtacg gtttgtttga
     9001 cgcccaaatc ctgcgcaatc cagcgggcac gttccagctc gacggcatgg cgttgcccgt
     9061 attggaaagt aatggcttgg acgttttcgc gcccgtagat ttggattgcc tgaatcaggc
     9121 aggtggtcga atcctgaccg cccgaaaaga tgaccaaggc tttttggttt gacatatcga
     9181 atcctagttt tggtaacgcg ggaggtttgc gaaccgcgaa gttggacagt aaagacgcgg
     9241 attatacagg caaacgcgtt cagacggcat ctgaacgcca tgccgtctga acacgcggct
     9301 tatgccgact tgatggcgag ttccgccaag cgtttgcttt gtgcgaaggc gatattgttt
     9361 tcttcggcgg tcaggacggg agccgctgtc gggaatatct tttttgacgg cttcgcagac
     9421 ggcggagact ttggggacgg tgcgcaatac ggcttcgcaa ccttcaacgc tgtcgatgcc
     9481 gcgagcgatt cggcgggcgg gattgcgggt gctaccgttt tggaaatagt agaggacgag
     9541 gattttgagg gggatttggg ttcatgggcg gattccgtta aaatggttcg gtcttcagac
     9601 ggcataacag ttgttccgat gccgtctgaa acagccgcag ttcaggcatt tgttcctgac
     9661 ggcaaaattg gctataacat cgggcatcaa tgcccaactt actgaccggg caagaaatta
     9721 tgaccttttt acaacgtttg caaggtttgg cagacaataa aatctgtgcg tttgcatggt
     9781 tcgtcgtccg ccgctttgat gaagaacgcg taccgcaggc ggcggcaagc atgacgttta
     9841 cgacgctgct ggcactcgtc cccgtgctga ccgtgatggt ggcggtcgct tcgattttcc
     9901 ccgtgttcga ccgctggtcg gattcgttcg tctccttcgt caaccaaacc attgtgccgc
     9961 agggcgcgga catggtgttc gactatatca atgcgttccg cgagcaggcg aaccggctga
    10021 cggcaatcgg cagcgtgatg ctggtcgtta cctcgctgat gctgattcgg acgatagaca
    10081 atacgttcaa ccgcatctgg cgggtcaatt cccagcgtcc gtggatgatg cagtttctcg
    10141 tctattgggc tttactgacg ttcgggccgc tgtctttggg cgtgggcatt tcctttatgg
    10201 tcggctcggt acaggatgcc gcgcttgcct caggtgcgcc gcagtggtcg ggcgcgttgc
    10261 gaacggcggc gacgctgacc ttcatgacgc ttttgctgtg ggggctgtac cgcttcgtgc
    10321 caaaccgctt cgttcccgcg cggcaggcgt ttgtcggggc tttggcaaca gcgttctgtc
    10381 tggaaaccgc acgttccctc ttcacttggt atatgggcaa cttcgacggc taccgctcga
    10441 tttacggcgc gtttgccgcc gtgccgtttt ttctgttgtg gctgaacctg ttgtggacgc
    10501 tggtcttggg cggcgcggtg ctgacttctt cactctccta ctggcgggga gaagcgttcc
    10561 gcaggggctt cgactcgcgc ggacggttcg acgacgtgtt gaaaatcctg ctgcttctgg
    10621 atgcggcgca aaaaaaaggc aaagccttgc ctgttcagga gttcagacgg catatcaata
    10681 tgggctacga cgagttgggc gagcttttgg aaaagctggc gcggcacggc tacatctatt
    10741 ccggcagaca gggttgggtg ttgaaaacgg gggcggattc gattgagttg aacgaactct
    10801 tcaagctctt cgtttaccgt ccgttgcctg tggaaaggga tcatgtgaac caagctgtcg
    10861 atgcggtaat gacaccgtgt ttgcagactt tgaacatgac gctggcggag tttgacgctc
    10921 aggcgaaaaa acagcagcaa tcttgagtat ttttgaaact gtatttttat cctaaagaca
    10981 ttttcctatc tgctgattcc accgtttcct ttctgtgtgc cgccataatt tttatgatag
    11041 acaagccatt caagtttgca ttgactgctt tttcattggc aatatccaaa tggctggtcg
    11101 ctgcatctaa aaataggatt ttcggttcgc aatataaggc ccgcgccaat acgatgcgtt
    11161 gtttttgtcc gcctgacagt gcgctgccca tatcgccgat caaggtttca tagcccatag
    11221 gcatggcgca gatttcttcg tgaatcatag caaggcaggc gcaatgttcg atttttcctc
    11281 tatccggttc cgtatcgaaa aaaca
//
LOCUS       DAWKST010000060        10536 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.060, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000060 DAWKST010000000
VERSION     DAWKST010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10536
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.060"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            213..395
                     /locus_tag="WJL87_001080"
     CDS             213..395
                     /locus_tag="WJL87_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799510.1"
                     /translation="MFWYVIGFCSFVVALLSLWVNVGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPDTEEKQQ"
     gene            complement(457..1458)
                     /locus_tag="WJL87_001081"
     CDS             complement(457..1458)
                     /locus_tag="WJL87_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ7799511.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEINV"
     gene            complement(1535..5368)
                     /locus_tag="WJL87_001082"
     CDS             complement(1535..5368)
                     /locus_tag="WJL87_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEZ7799512.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETSQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEVAPLVRALIELDLVIIPRGGGTGYTGGAIPLDAMSAVIN
                     TEKLDKHRGVEYVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWQMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGLNIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITGVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVAAVKERWDWLLANLDTPLADYKARYGAA
                     VHAAPEAKDNESCFTAFRDFRLRVSVKADVMKPLAEIFSGKTDTKIIQGLGKIHAKTV
                     RGRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     AMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVQGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYTDDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5629..5725
                     /gene="ffs"
                     /locus_tag="WJL87_001083"
     ncRNA           5629..5725
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WJL87_001083"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5893..7584
                     /gene="dld"
                     /locus_tag="WJL87_001084"
     CDS             5893..7584
                     /gene="dld"
                     /locus_tag="WJL87_001084"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ7799513.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPSEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            7716..7823
                     /locus_tag="WJL87_001085"
                     /pseudo
     CDS             7716..7823
                     /locus_tag="WJL87_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7879..8250
                     /locus_tag="WJL87_001086"
     CDS             7879..8250
                     /locus_tag="WJL87_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ7799514.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            8405..8953
                     /locus_tag="WJL87_001087"
     CDS             8405..8953
                     /locus_tag="WJL87_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ7799515.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDDELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            9088..10179
                     /locus_tag="WJL87_001088"
     CDS             9088..10179
                     /locus_tag="WJL87_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ7799516.1"
                     /translation="MNAQTLIANVAEFVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRAVKDK
                     YLADILQGKVLAGTGMSNTVKHLADIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANVVNAEVNRYLDDGYDSLKTRLD
                     GAWAETERLAGLAWSGTPDNLTTLKLREAAAVLTLAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
BASE COUNT         2443 a         2889 c         2853 g         2351 t
ORIGIN      
        1 gcggtttgct ggaagaaggt gctcatgaga aatcccctaa atgtcttagt aggaatttag
       61 gggattttga ggaattttgc aaaggtctct atttgtactg tcttcggctc gccgccttgt
      121 ccttatttaa atttaattca ctatatttgc ccgtgcgttt gtgataaatt cggcactgta
      181 tttgatatga ttttaacaaa tcgggagggg caatgttttg gtatgtaatc ggcttttgct
      241 cctttgtcgt cgcgctgctg tcgctgtggg tcaatgtcgg cgcattcggt atgcaggaag
      301 acgatacccc gcaatcggat tatgaaaaac gtttgggctt gggtgcgaag ctgaaaaaca
      361 aaaatacgcc ggatacggaa gaaaagcagc aataaagata tgaaatgccg tctgaaagat
      421 tttcagacgg catttgcttt gaagcatggg agacagtcaa acatttatct catgctcaaa
      481 tgcgttcagg attcggcgga cttcatccgc atccttaacc gttcggacgc ggtcaaacag
      541 cgtttgcgcc gggtcgaacg tttttttcat catgcccaac cattgcttaa gacgggcgat
      601 cgggtattta ttgtttgcct cttttgtcag gcacagctcg aaaaactgcc gtatccattt
      661 agaaacttcg gcaaaatccg tgtctttgac cggcccgccg ttctcgtatt gcttgatttg
      721 ccgcgccaaa tcggggcgga tgaccgcgcc gcgaccgagc atcacgctgt tgcagccgct
      781 gattgttttg atgccgatat agtcttgcag gctgaaaacg tcgccgttgg cggtaacggg
      841 aatattgacg ctgtctcgga ttttccttat ccattcccaa tgcgccggcg gttcgtaacc
      901 ctcggctttg gtacgcgcgt gtacggtcag tccgcatgcg cccccttcgg caatcgcaca
      961 ggcgcattcc aaagcccggc ttttgtcttc gtaaccgagc cgcatttttg cggtgagcgg
     1021 aatatgtgcg ggcaaacgtc cgcgcagcgt tttgacgatg tggaatatca gttccggctc
     1081 ttttaaaagg attgcgccgc ctttgtgttt gttgacggtg ggcgcggggc agccgaagtt
     1141 caaatcgatt ttgttcgcac cgaagcggac ggcttccagc gcattcgccg ccatattgtc
     1201 cgcatcgctg cccaaaagtt ggacggtgca gggcgtgccg gaaaacgttt tgtttccgtt
     1261 ggcgatttcg gggacatatt ttaaccatat ggatcgggaa tgcacggtat gggtaatgcg
     1321 tacaaattcg ctgacgcatt cgtcgtagcc gccaatacgc gtcagcaggt cgcgcatcac
     1381 gtcatcgacc agaccctgca taggggcaag gatgattcgg gttttttgtt tgggttcggt
     1441 tgtttgtccg tcaatcatga ttcgggtagg tatttgattc aaacaatgcc gtctgaaggc
     1501 tttcggcgtt cagacggcat ttccgtgtcc gttgttacag caatactttt tccacgccgc
     1561 cgttgttggc ttttttcaca aactcgtcta gccagttttc gccgaggatg tgtttcgcca
     1621 tttcgatgac gatgtagtcg gcaggcatat tgttgtcgtc ggtgtagcgg ctcaagcctt
     1681 gcaggcaggc tgggcaggaa gtaagcattt tgacgggttc accctgcggc agctctttga
     1741 ggtttttctc gatttcctct tgtttgcgga atttgacttg ggtggcgata tcgggtcgtt
     1801 tgacggcgaa cataccggac tcgccgcagc agcggtcgct taagacgact ttctgcccca
     1861 tcaggctgct ggccatttgg gtggcgttca tggttttgat gggggtgtgg caggggtcgt
     1921 ggtagaggta ttgctgacct tgcacgccgt cgagtttcac gcctttttcg agcaggtatt
     1981 cgtggatgtc gatgatgcgg cagccgggga agatttcttc aaagcggtat ttttcgagct
     2041 ggtcgtaaca agtgccgcaa ctgacaacga cggtcttaat gtcgaggtag ttgagggtgt
     2101 ttgccatacg gtggaaagcc acgcggttgt tggtgctcat ttcttcggct ttagccttgt
     2161 tgccgcccgc gtcttgcgga tagccgcaac acatatagcc gggcggcagg acggtttgta
     2221 cgccgacgtg ccagagcatg gcttgaacgg caagtccgat ttggctgaac aggcgttccg
     2281 aaccgcaacc cgggaagtag aacacggctt cggcatcttc gggcgcggcg gggttgcgga
     2341 tgatggggat gcttttgccg tcttcgatgc ccaataagga gcgcggtgtt ttggcgggta
     2401 cgcttttcgg caaaggacgg ttgatgaaat ggataacctg ttctttaatt ggggctttgc
     2461 cgacggttgc cttgggttcg gctttttgct ttttcgtgcc gatcggaaga agcttgccga
     2521 ttttgtaggc aaagttttgc gctgggaagc cggtctgtat catggcggcg cgaagggctt
     2581 tgatggtttt cgggccggtg gcgttcaaaa acgccatacc catagctgcg gcaggcgcaa
     2641 atcgtttgtg gccggaatcg gcaaggtagt tgcggacggc tacggttacg tcgccgaagt
     2701 cgatgttgac ggggcagggt ttgacgcagc ggtggcacac ggtgcagtgg tcgccgatgt
     2761 ccatgagttc ttcaaagtgt ttgacggaaa cgccgcggcg ggtttgttct tcgtataaga
     2821 aggcttcggt cagcaagccc acgccgagga ttttgttgcg cggactgtac agcaggttgg
     2881 cacgcggaac gtgagtagag cagacgggtt tgcatttgcc gcagcgcagg cagtctttga
     2941 cggaatcggc aatcgtgccg aggtctgatt tttccataat cagcgattcc gcgcccaaaa
     3001 gctcgaaaga cggcgtgtag gcgttgcgta agtccgagcc tttcatcagt ttgtggcggt
     3061 tgaaggtgtg tttgggatcg acttggtttt tgtagtccca aaacggctgc atttcttcat
     3121 cgcttaaaaa ttcgagcttg gtgatgccga tgccgtgttc gccggaaatt acgccgccga
     3181 gcgaacgggc gattttcata atgcgttcga ccgaacggta ggctgtctga agcatttcgg
     3241 catcgtctga gttgaccgga atattggtgt ggacattgcc gtcgccggcg tgcatatgca
     3301 gggcgacaaa gactctgccg cgtacggttt tggcgtggat tttgcccaag ccttggataa
     3361 tcttggtatc ggttttgccg ctgaagattt cggcgagtgg tttcattacg tctgctttga
     3421 cagacacgcg caggcggaaa tcgcggaatg cggtaaagca gctttcattg tctttggctt
     3481 cgggcgcggc gtggacggct gcaccgtagc gggctttgta gtcggcaagc ggcgtgtcca
     3541 aattggcgag caaccagtcc caacgttctt tgacggcggc aacgtgggcc aaggcgtgtt
     3601 tgccgcgttc gcccaaaagt tcggcggtcg gcaggtcagt gcccattttg tcgatgggga
     3661 gtttgcccga aagatattgc tccaaggcgg cacagagttt gagcttgttt tggatggaaa
     3721 gctcaatgtt gatgcgttcg atgccgtccg aatactcgcc gagcctttcg agcgggatga
     3781 ccacgtcttc gttaatttta aaggcgttgg tgtgcttggc gatggcggcg gtgcggctgc
     3841 ggtcgagcca aaaggttttg cgcgcttcgg gagacacggc gataaagcct tcgccatcgc
     3901 gggcgcgggc aagttcgcaa atgtgttcgg cggctgcctc tacggcggct tcgtcgtcgg
     3961 aaaccacgtc cgccagcaag accattttcg gccgtccctt gcccgccgct ttggttgcgt
     4021 agccgacggc gcggacgtaa cgccagtcca aatgttccaa acctgccagc cgaacgctgt
     4081 cgtgggcgag caggaaatcg cggatttcga cgatagaagg cgtagcggtg gcgaccgtgc
     4141 cgaaaaactc catacacaca gtgcgcgtgt atttcggcat tttgtgcaac acgaaagcaa
     4201 cgccggtgat gatgccgtcc gtgccttctt tctgtacgcc gggcaggccg ctcaagaatt
     4261 tgtcggtaac gtctttgccc aaaccgactt tgcgaaattt gtggccggga atttccaaac
     4321 gttcggtttt aacgatattg aggccgtctg aatccagcgt atgtacgtca aacacggcgg
     4381 tttcttcatc gtggattttg ccgaaattgt ggcgcacgcg ctcgatacgc agccattcgc
     4441 cttgagggtt gaccatctgc cagtaggcga ggttgtccaa cgccgtcccc cacaacacgg
     4501 cttttttgcc gcccgcgttc atcgccacat tgccgcccac gcaggacgca tcggcagaag
     4561 tcggatcgac ggcgaacacc aaacctgcct gatgcgcggt ttcttccacc cgccgcgtaa
     4621 ccacgcccgc gccgcaccgg ataatcggat gcttgccgtc caatcctgcc agctcaacgt
     4681 attccacgcc gcgatgcttg tcgagctttt cggtattgat gaccgcgctc attgcgtcca
     4741 aaggaatcgc gcctccggta taacccgtgc cgccgccgcg cgggataata accaagtcca
     4801 attcgattaa agcgcgcacc aaaggcgcga cttccgcctc cgtgtcggga ttgacgacga
     4861 caaacggata ctccacgcgc cagtcggttg catcggtaac gtgcgttacc cgcgccagcc
     4921 cgtcgaacat aatattgtgc ggcttggtga ttttgctcaa acgctccaaa atctgccgcc
     4981 gtttttggct ggtttcatca aaactgctat caaaacgctc gactgctttt tctgctgcgg
     5041 caaccaacac atccacttgc cgattatcgt cgcggcgttt gcggatttca tttaagcggt
     5101 ggcgcatttc acgtaccagc gcggcgcggc gttttgggtg ctccagcaag tcatcgacca
     5161 gatacggatt gcgcacgacc acccaaatat cgcccaacac ttcaaacagc atccgcgccg
     5221 aacgcccggt tttgcgctgt ccgcgcaagt cttgcaggat ttgccacgcc tcatcgccca
     5281 gtaggcggat gacgatttcg cggtcggtgt aggaagtata gttgtaggga atttcccgaa
     5341 tacgctgagg ggcggtagtc gtggtcatgg tgtgtccgtg tttggcagtt tttatttgaa
     5401 tgccgtctga aaattcggtt tcaggcgggt ttgaaaaggc gataatgtag ttcaattttt
     5461 gatatttttc aatgccgaaa caggaaatac ccttatagaa acaaaataac gctttgagaa
     5521 attgttgaca atattatcaa atataaattt taggtggtgt ttccggcgta tgaccgtatc
     5581 aaaacggttg agtttttcag aaaacccgtt ataatgccgt ttctctgagg cgggtctccc
     5641 cgcatggcaa atcggaacac cgggtcaggg gcggaagcca gcagcccact ccgatgcgcc
     5701 agtgccgggg gtttggcccg ccgccctatt tgaaacgccg gagctttgat gttccggcgt
     5761 tcccgtatcg caccgttgcc gtctgaaagc cgttcagacg gcattttctt tactttgcca
     5821 taacgtataa ctacaatgtg aaaacaattc caatgtgcat atgcacctgc cgacaatttt
     5881 aaggaaactg ccatgagcgc atcacaatta ctgtcaagac tgacccaaac cgtaggcgaa
     5941 aaatacatca tcaccgaccc cgcgaaaacc gaacaatacc gccaaggcta ccgcttcggc
     6001 gagggtaagg cgttggcggt ggttcgcccc ggaagcattc tggaaatgtg gaaaattctg
     6061 caggcgtgcg tcgaagcaga cgtgattgtg attacgcagg cggcgaatac cggactgacc
     6121 ggcggttcga cccccgacgg caacgattac gaccgcgaca tcgtgattgt gaacaccatg
     6181 cggatgaaca tcatccaaac catcaacaac aacgaacaag tcgtctgtct gcccggttcg
     6241 accctgaacc agcttgaatt gctgctgaaa cctttggggc gcgaaccgca ttcggtcatc
     6301 ggctcatcct gtatcggcgc gtccgtcttg ggcggcgtgt gcaacaactc cggcggcgcg
     6361 ttggtacagc gcggcccggc ctacaccgaa atggcgttgt tcgcccaaat caacgaagaa
     6421 ggcaggttgg aactggtcaa ccacttgggc atagacttgg gcgacacgcc cgaagaaatc
     6481 ctgaccaacc tgcaaggtca tcattatcaa aacaaagaca tcaaacaaga cgcgggcaaa
     6541 ggacacgacc acgcctattg cgaacacgtc cgccaagtgg acgaaccgac cgccgcgcgt
     6601 ttcaatgccg accccgcccg ccattacgaa gcctcgggct gcgcgggcaa actgatggtt
     6661 tttgccgtcc gtttggacac cttcccgcaa gaaaaacaaa ccgccgtgtt ctacatcggc
     6721 acgaacgaca tcaacgagct gaccgacatc cgccgcgccg ccttgggcga atttgaaagc
     6781 ctgcctgttt ccggcgaata catccaccgc cacgctttcg atattgccga cgtgtacggc
     6841 aaagacacgt tttacgtcat caaaaaattc ggtacgcacc aactgccgaa attatttgac
     6901 ttgaaggcgc gcgtggacag gttcggcaaa aaagccagct tcctgcccaa acatttttcc
     6961 gacaaggcca tgcagttcgt cagcaaatac ctgcccgacc acctgcccaa atccatgcgc
     7021 gattaccgcg acaaatacga acaccacctg attctgaaaa tgggcgggaa aggcgtggat
     7081 gaggcgcgcg cgttcttaaa agaatatttt gcacaccaca gcggcgcgtt tttcgagtgc
     7141 aacgccgaag aaacccaagc cgcgatgctg caccgtttcg ccgtcgcctc cgccgccatc
     7201 cgctaccgcg ccgtgcatga tgacgaagtg gaagacttgg tcgcgctcga catcgccctg
     7261 cgccgcgacg atcgcgactg gtttgaaaaa ctgccgtcgg aaatcgacaa taaaatcatc
     7321 cataaactct actatggaca tttcatgtgc cacgttttcc atcaggatta catcatcaaa
     7381 aaaggcaacg actgcatggc gttggaacac gaaatgctgc atcttttaga ccaacgcggc
     7441 gcgcaatatc ccgccgaaca caacgtcggt catttgtatg aagccaaacc cgcgctcaaa
     7501 cagttttacc gcaaacttga cccgaccaac agcttcaacc cgggcgtagg caaaaccagc
     7561 aaaaagaaaa actgggcgga ataagcgcgt ccgctttgaa ggcaggcaat gccgtctgaa
     7621 ggcaaaacgc cgttcagacg gcattgtcgg gtttgaagtt ccccatcggg taatcgcttt
     7681 caagccgtat gccggcacag gcgcgcgcgc ctcccaaata tagtggatta aatttaaatc
     7741 aggacaaggc aacgaagccg cagacagtac agatagtacg gcaaggcgag gtaacgccgt
     7801 actggtttaa atttaatcca ctataaagtc tgatcctacc ttcccaaagg gcagggtttc
     7861 aaccgaaaag gaaacacgat gaaaccatac aaaatctaca cccatcccgc cctgccgccc
     7921 caagccgtca aacaaggctg gtcgtggccg ggccttttgt tcggcacgct gtgggcgtgt
     7981 ttcaaaagaa tgtggggttt ggggctgggg ctgacgggcg cgatattcgt gctggcggtg
     8041 tttgcccaac tggtttacgg cgacacgccc gccacagact cggcgtttaa cgtattgggc
     8101 ttggcggttt ccgtctggtt cggcgcaaag ggcaacagcc tttatgcacg ccacctttta
     8161 tcgcgcggct ataccgaatt gcccgaaacg gttgaggcag ccaatcccca agccgcattg
     8221 gcgcaatatt tcgggcgcgg gggccgataa gcgcgttttc ttttccatca tgccgtctga
     8281 aaaatgttca gacggcattt cttataacct tttgtttgca gcacataacc aatcagtctt
     8341 tcctattccg ttccgaaatg cataccatgc gcccgtaccc aatatttgcg aacaaggaaa
     8401 gaaaatggca cgtttaaccg tacacaccct cgaaaccgcc cccgaagccg ccaaagcgcg
     8461 cgtcgaggcg gtacttcaaa acaacggctt tatccccaac cttatcggcg tattatcaaa
     8521 cgcccccgaa gccttggcgt tttaccaaga agtcggcaag ctcaacgccg ccaacagcct
     8581 gaccgctggc gaagtcgaag tcatccagat catcgccgcc cgtaccaacc aatgcggttt
     8641 ttgcgtggca gggcacacca aactcgcaac cctgaaaaaa ctcctgtccg aacaatccgt
     8701 caaagccgcg cgcgctttgg cagcaggcga atttgacgat gccaaactcg gcgcgctcgc
     8761 cgccttcacc caagccgtaa tggcgaaaaa aggcgcggta tccgatgacg aactcaaagc
     8821 atttttcgac gcgggctaca accagcagca ggcagttgaa gtcgtgatgg gcgtagcctt
     8881 ggcaactttg tgcaactacg tcaacaacct cggacaaacc gaaatcaacc ccgaattgca
     8941 ggcttacgcc tgatacggca aaccgcccga atatcgggcg gtttttcaaa aacaccccct
     9001 caaacaaaaa caagccgcct aagcggcagg gaagcagccg cgcatcaggc gcgcccccga
     9061 tttccccgca aaccttaagg aacaaagatg aacgcccaaa ccctgattgc caacgttgcc
     9121 gaattcgtca aaaccaagct caaacccata gtggacgaca tcgaccgcaa aggatactac
     9181 cccgaagcat ttatgcgcga actcggcgca atcggtggat tcggcgcagt cggtacagaa
     9241 gccgaaggcg gcaacggctt gggtttggca acgcaaatcg ccgtattgcg cgaaatcggc
     9301 aaagaatgcg gcgcaacctc tttcagtgcg tggtgtcagg cggcttgcgc gtggtatctg
     9361 caccaaacgc ccaaccgggc cgtcaaagac aaataccttg ccgacatcct gcaaggcaaa
     9421 gtattggcgg gtacgggcat gtccaatacc gtcaaacacc ttgccgacat cgaaaaacac
     9481 aacctccaag ccgagcgcgt ggagggcggc tacacagtca acggcgcgct gccgtgggtg
     9541 tccaacatcg gcgaagacca catttgggcg aataccgccc aaatcggcgg cggctacgtc
     9601 atgttcatca caggcggaca atgggaaggc gtaagcctgc aaaactgccc cgaattttgc
     9661 gccctcgaag gcacgcgcac cttcagcctg aacttcaaag acgtatttat ccccgacgaa
     9721 gacatcatcg ccgcgcccga acagtttgcc gactacatcc aaagcatcaa agcaggcttt
     9781 atcctcctgc aaatcggcat cggcgcgggc gtgatagacg gcagcctcgg catcatccgc
     9841 ctcgccaacg tcgtcaacgc cgaagtcaac cgctatctcg acgacggcta cgacagcctc
     9901 aaaacaaggc tggacggcgc gtgggcagaa accgaacggc tcgccggatt ggcttggagc
     9961 ggcacgcccg acaaccttac caccctcaag ctgcgcgaag ccgccgccgt actgacactt
    10021 gccgccgccc aatccgccgc cctgcattcc ggcgcaaaag gctacctgat gcggagtccc
    10081 gcccaaaggc gcgtccgcga agcaatgttt gtcgccatcg taactcccgc gatcaaacac
    10141 ctccgcaaag aaatagcggc aatcgaagcc gcaaaataaa caggcggaaa aaaccgaaac
    10201 ccccatcgcg tcattcccgc gaaagcggga atccagcccc caacgcaaca ggaatccatc
    10261 ggaaaaaaca gaaaccccca aaccgtcatt cccgcaaaag cgggaatcca gccccctaac
    10321 gcaacaggaa tccatcgaaa aaaccgaaac ctccaaaccg tcattcccac ggaagtggga
    10381 atccagtaac cgaaaaacca caggaatcta tcgaaaaaaa acccaacccc caaaaaaacc
    10441 gggcggatac ctcaccatcc tcccaaaccc tgatttatag cggatgaaca aaaatcaggg
    10501 caagacggcg aaaccgcaga cagtacaaat agtacg
//
LOCUS       DAWKST010000061        10533 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.061, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000061 DAWKST010000000
VERSION     DAWKST010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10533
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.061"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            54..1064
                     /gene="waaF"
                     /locus_tag="WJL87_001089"
     CDS             54..1064
                     /gene="waaF"
                     /locus_tag="WJL87_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ7799517.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAIIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLNLGKPVLAFCPGAEFGPAKRWPTRHFAELGKHYLAAGWQVWLFGS
                     QKDDEIAEEINRLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            1119..1565
                     /gene="smpB"
                     /locus_tag="WJL87_001090"
     CDS             1119..1565
                     /gene="smpB"
                     /locus_tag="WJL87_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ7799518.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            1996..3981
                     /locus_tag="WJL87_001091"
     CDS             1996..3981
                     /locus_tag="WJL87_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEZ7799519.1"
                     /translation="MISLFFSILYTSPLLAVDYVYDNSKLTNEQIDRLKKLRDKSSEY
                     WKQETYLLKPTNGSSQNSLKVPALYSKTHQFENINNSKKISFYDKEYTEDYLVGFARG
                     FGVEKRNGEEEKTLQQYFKDCVNTGNSNNDQCKQPSLGSYVPILIKSDIFSLGPQIKN
                     SHINSEILSVGNYIEWLRPTLNKLTGWQEQLYSGLDPFHYIEVTDNSHVIGQTISLDQ
                     FRLENSLWEPRWDSDVGELKTTNADIRFNTKSESLLVKEDYAGGARFRFAYGLKDKVP
                     ETPVLTFEKNITGTSDIIFENPIDDLKSLDGHQIIKVNGTADKHAFRLSGKHQKGIYT
                     LSLQQRPEGFFTKVQERDDIAIYAQQAQAANTLFALRLNDKNSDIFDRTLPRKGLWLR
                     VIDGHSNQWVQGKTAPVEGYRKGVQLGGEVFTWQNESNQLSIGLMGGQAEQRSTFRNP
                     DTDNLTTGNVKGFGAGVYATWHQLQDKQTGAYIDSWVQYQRFRHRINTEDATERFTSK
                     GITASIEAGYNALLAEHFTKKGNRVRFYLQPQAQLTYLGVNGKFSDSENAHVNLLGSR
                     QLQSRVGVQAKAQFSLYKNIAIEPFAAVNALYHNKPFGVEMDGERRVINNKTAIESQL
                     GVAVKIKSHLTLQATFNRQTGKHHQTKQGALNLQWTF"
     gene            complement(4162..5529)
                     /locus_tag="WJL87_001092"
     CDS             complement(4162..5529)
                     /locus_tag="WJL87_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ7799520.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKFPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAIPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(5639..5920)
                     /locus_tag="WJL87_001093"
     CDS             complement(5639..5920)
                     /locus_tag="WJL87_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799521.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPATEAPAAEAPAAEAAAT
                     EAPAAEAPTAEAAATEAPAAEAPAAEAAATEAPAAEAPAAEAPAAEAAK"
     gene            6393..6875
                     /locus_tag="WJL87_001094"
     CDS             6393..6875
                     /locus_tag="WJL87_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ7799522.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            6962..7774
                     /gene="hpnC"
                     /locus_tag="WJL87_001095"
     CDS             6962..7774
                     /gene="hpnC"
                     /locus_tag="WJL87_001095"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ7799523.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLQRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEGQIAEGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(7845..8315)
                     /locus_tag="WJL87_001096"
     CDS             complement(7845..8315)
                     /locus_tag="WJL87_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ7799524.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIIEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHKIA"
     gene            8406..10247
                     /locus_tag="WJL87_001097"
     CDS             8406..10247
                     /locus_tag="WJL87_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ7799525.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTNPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEVGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANKP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
BASE COUNT         2640 a         2276 c         2869 g         2748 t
ORIGIN      
        1 ttttccaacg gtttcgcggt aaaattctgc ctgtctgatt tcggtttctt gttatgtcca
       61 tcaaaatcct gattatttct cccagttgga taggcgactg cgtgatgacc cagcccttgt
      121 tccgccgttt gaaggaactt caccccggtt gcacgattga tgtgttcgca ccgaagtggt
      181 cgatggcggt gtttgagcgt atgccggaag tgaatgagat tcttgaaaat ccgttcggac
      241 acggtgcgtt ggagttgaaa cgccgctggc gggtcggtag ggatttgggg cggcgcggat
      301 acgatcaggt tatcgtgttg cccggttctt tgaaatcggc aatcatcgcg ctggcgacag
      361 gtatcggtaa aaggacgggt tatgtcggtg aaagccgtta ttttctgttg aacgatatac
      421 gcaggctgga taaggaacgt ctgcctttga tggtggacag atatacggct ctcgcgcatc
      481 cgagccagga ggattttgac gggcattcgg gattccccga gttttccatt gatgaacggc
      541 ggcgggaaat ttctgtcgaa actttcggtt tgaatcttgg aaagcctgtt ctggcttttt
      601 gtccgggtgc ggaattcggg ccggcaaagc gttggccgac aaggcatttt gccgagttgg
      661 gcaaacatta tctggcggcg ggttggcagg tttggttgtt cggttcgcaa aaagatgatg
      721 aaattgccga ggaaatcaac cgcctttcag acggtatgtg tgtcaatttg tgcggcaaaa
      781 ccgatttgtc gcaggcaatg gatttgctgt cgttggcgga cacggtcgtg tgcaacgaca
      841 gcgggctgat gcacttggcg gcggcattgg gcaggaaagt ggtggcggtt tacggttctt
      901 ccagcccgac gcatacgccg cctttgagcg acagggcgaa aatcgtcagc ctacacttgg
      961 aatgttcgcc ctgtttcaaa cgtgaatgtc cgttggggca taccgactgc ctcaacaggc
     1021 tgtatcccga gaagattgtg caggcggttg aagaggcggt atgatgggtt ctgttttcgg
     1081 gtttatgccg tctgacggcg gatattgata ggatgtttat ggctattgcg aacaataaaa
     1141 aagcatttca cgattttttc attgaagacc ggattgaagc cggtttggtc ttggaaggct
     1201 gggaagtcaa agcaatccgc gccgcgcgcg tacagcttaa agagagttat atctattgga
     1261 aaaaagacgc gttttatctg gtcggctgcc atattacggc tttgcccaca gcctcgacac
     1321 acattaaacc agatgccgta cgtccgcgca agctcttgtt gaaccagtcg gaaatcaaca
     1381 aattaatcgg caaaaccgaa cgtgccggct acaccattgt gccgctggat ttgcattttt
     1441 cgcgcggaaa aatcaagatg gaaatcggtt tggcaaaagg taagaaacag cacgacaaac
     1501 gtcagagtat gaaagaggcg gattggaaac gcgagaaaca gcggttgatt aagcatacgc
     1561 gctgattgtg gaaacagtgc ttcagacggt atgggcaagg ctgccgcctg atgtgtaaac
     1621 gttctacacc taaaccagca tccgctttaa tcagcacggc atgatgcccc aaaccttcgt
     1681 caaaggtttg agcaatttga tgataaattg gcattagcca agggtaaagt gcggtcattt
     1741 ttcctgaaat ttttgtaaat aacgagggca attatacaca ttttcaaact ggcttgacaa
     1801 aatagcaaaa agcaaacgtt tacttttaat aaataagaaa taaatagaga aaaatgtgag
     1861 ctaaatgaca ttttttgttc aattctatgc taaaatccac gccccattta tctttacttg
     1921 acaaaggaaa atttattatg aaactcgaag caagcaagca agcaagcaag caagcagaag
     1981 tttaaaaaat catttattat aagtctattt ttttctattc tttatacctc tcctcttttg
     2041 gctgttgatt acgtttacga caattctaaa ttaacaaatg agcaaattga tcgcttaaaa
     2101 aaacttagag ataaaagcag cgaatactgg aaacaagaaa cttatcttct aaaaccgacg
     2161 aatggtagct ctcagaactc tctcaaagtt cccgcactat actctaaaac acaccaattc
     2221 gaaaacatca acaactcaaa aaagatttct ttttatgaca aggaatacac tgaagattac
     2281 cttgttggct tcgcccgagg tttcggggtt gaaaaaagaa atggggaaga agaaaaaaca
     2341 ctccagcaat attttaagga ctgtgtaaat actgggaatt ccaataatga tcagtgtaaa
     2401 caaccatcct taggaagcta tgtcccgata ctcataaaga gcgatatttt ctccttaggt
     2461 ccacaaataa aaaatagcca tatcaacagc gaaattttat ctgtaggtaa ttatatcgaa
     2521 tggcttcgcc caacacttaa taagcttact ggttggcaag aacaactata ttctggttta
     2581 gatccattcc actatattga ggttacagac aactcgcacg ttatcggaca aacaattagt
     2641 ttggatcaat tcagattaga aaattctctc tgggaacccc gttgggattc tgatgttggg
     2701 gaattaaaaa caacgaatgc agacatccgt tttaatacta aaagtgaaag tttactcgta
     2761 aaagaagatt atgctggcgg agctcgtttt cgttttgctt acggtctaaa agataaagtc
     2821 cctgaaacac cagttttaac ttttgaaaaa aatataactg gcacttccga catcatcttc
     2881 gaaaatccaa tagacgattt aaaaagcctc gacgggcatc aaattatcaa agtaaatggc
     2941 acagcagata aacacgcatt ccgtctttct ggcaaacacc aaaagggaat ttatacgctt
     3001 tctttacaac aacgcccaga gggctttttt accaaagtgc aagaacgcga tgatattgcg
     3061 atttatgcac aacaggctca agccgccaat accttattcg ccttgcgttt gaacgacaaa
     3121 aacagcgata tttttgaccg cactttacca cgcaaaggct tgtggttgcg tgtgattgac
     3181 ggacattcca accaatgggt gcaaggcaaa acagcacctg tagaaggcta tcgtaaaggt
     3241 gtgcaactcg gtggcgaggt gttcacatgg caaaacgaaa gtaatcaact ttctattggc
     3301 ttaatgggcg gacaagcaga acaacgcagc actttccgta acccagatac agacaatctt
     3361 acaacgggaa atgtgaaagg ctttggtgca ggcgtttacg ccacttggca tcagcttcaa
     3421 gataaacaaa caggggcata tattgacagc tgggtacaat atcaacgttt ccgccaccgt
     3481 attaacactg aagatgctac agaacgtttt acttcaaaag gtattactgc ctcaattgaa
     3541 gcaggttaca atgctttatt ggcggaacac ttcactaaaa agggcaaccg tgttcgtttt
     3601 tacctgcaac cacaggcaca attgacttat ttgggggtaa acggaaaatt cagcgatagc
     3661 gaaaatgccc acgtgaattt acttggctct cgccaattac aaagccgagt gggcgttcaa
     3721 gctaaagctc aattttcact ctataaaaat atcgctattg aacctttcgc cgctgtgaac
     3781 gcgctttacc acaacaaacc tttcggtgtg gaaatggacg gagagcgtcg agtgataaac
     3841 aataagacgg caattgagag ccaattaggt gtagcagtga aaatcaaatc tcacttaacc
     3901 ttacaagcca cattcaaccg ccaaacaggc aaacatcatc aaactaaaca aggtgcgttg
     3961 aatttgcagt ggacgtttta agttaagttg taacaaaata aaatcggcag ggaaattcac
     4021 tgccgatttt tttattttaa aaatgcggtc atttttcgtg tcatttccac caatttttta
     4081 ccgcactcac gagctcgcgc taaaccgaca atcggatcta ttgtcccttt ctttgttgtg
     4141 tatgcaaatc agaaatgccg gttacggaag cagtttgccc ggattcataa tgttatatgg
     4201 atcaaggtgt tgtttgatgc ttttcatcag ggcgatttcg gcaggcgtgc gtactttgtc
     4261 cagccactgt tttttgatga tacctatgcc gtgttcggcg gcaatcgtgc cgttgcaggc
     4321 aagaacgttg cgataaactg cgtcgttgat gtcgttttcg taacgataga cttcattgct
     4381 gaggatttcg ggcaggaaag tattgtagtg caggctgccg tcgccaagat gtccgaagca
     4441 gacgatttgt atgcctttaa aattctgttc caaatctttg gcgcaccggc ggacaaagtc
     4501 ggcaacgcgt ccgataggaa tggcaatatc gtgtttgatg ctggtgccca gtttgcgttg
     4561 cgatgcggag atgttttcgc gcaatgccca catatggata cgttcttgtt cgctttgtgc
     4621 caatacgctg tcggtaaatc cttttttata aagaaattcg acaagccgat catcaagatt
     4681 gctgtcgggt aatgagtcgg tcaactcaag taaaatatgc cattctgaat gtgtcggcag
     4741 ggggaatttg ctgaattcgg aagacaactc ggcggcaaaa cggccgatca gctcaaaact
     4801 gcataggcgt tcggcaaagt gagcttgggt ttcggtcagc aggcggacgg cggattcgat
     4861 gtcgggtatg ccgacccatg ccgttgcctt gtccgaggga cgggcaaaaa gcttgagcgt
     4921 ggcggcagtg atgatgccca gtgtgccttc gctgccgata aacagatggc gcaggtcgta
     4981 gccggtggtg tttttatgca ggggatggag atgggaaacc agctcgccgt tggggaggac
     5041 gacttccaaa ccgataacca ggtcgcgcat cgtgccgtaa cgcaatacgt tcaaacctcc
     5101 ggcattgcag gcgatgttgc cgccgatttg acaggagcct tcgctggcga gactgagtgg
     5161 gaacagcctg tttgaggctt cggctgcctg ttggacggtt tggagtacgg aacctgcttc
     5221 gacggttatg cagttgtctg acaaattgat gctgcggatg cggttgagtt tggaaaggtt
     5281 cagcaatacg ccgttttccg atactgccgc gccgcacaaa ccggtattgc cgccttgcgg
     5341 cgtaaccgga atacggtgtt cgtaacaaaa acgcataatg gtttgcacgc tttcaacgct
     5401 gcgcggctgc aaaatgatgt ctggtgcaga cgtaaagcgg ttgcgctggt cttttaaaag
     5461 cgtcggagaa agttcggcaa tttcatctgc cggcaggagt cgggaaaatt cggtatgaag
     5521 gtcaggcatg tttgtcggaa gggtagggag aagattgagg aagggaatct gatgccgtct
     5581 gaaaaatcaa aaaagcagga tactgaacgt atcctgcttt tttgcaagcg gaaaatgctt
     5641 attttgcagc ttcggcagca ggagcttcgg cagcaggagc ttcagcggca ggtgcttcgg
     5701 tagcggcagc ttcagcagcg ggagcttcgg cggcaggtgc ttcagtagcg gcagcttcag
     5761 cagtgggagc ttcggcggca ggtgcttcag tagcggcagc ttcagcagcg ggagcttcgg
     5821 cggcaggtgc ttcggtagcg ggagcttcag cggctttttc accgccgcag gctgccagga
     5881 ccaaagacaa caaagcagcg gcaaacaggg attttttcat tttagtaacc tttagaacca
     5941 atttaaatta ttgaaacaaa taggtcttta tcgaccatac tacaaaccgg gttggaaatt
     6001 catcgctgaa tttgcgtaat tatgcactat ggaataaggg attgcaatgt cgtacagatg
     6061 ggaaaaaggt ttgaatgaaa agttttctgg cgggcggatt gataaatcct gtaaaacggt
     6121 tttgctgtta aaaattcgat agggataaat attatagaga ttaatataat ttcagtatct
     6181 tgtatgattt attaaggcgg attgggaaag tcggcagaca ggtcggaatg gttttaagaa
     6241 tgttgtaaaa aagttacacc gcaataaaat aacaaaaatt tacattgaaa tatgtgctgt
     6301 acagtttatg aacaggttaa cggtaatcgg ccggcgcggc ggttgtgtta gaatgcgcct
     6361 gtaatcaatc tgctgttcct gaaaggacaa atatggctat cgtgaaaaat tccgtggttt
     6421 cgctgcatta tgagatgtat gatgccaaca atcagctttt ggacaaaacc gaagaaccga
     6481 ttgcgtatct gcacggcggt tacgacggca ttttcccttt ggtggaagag gcgttgcacg
     6541 gtaaggatgc cggcgatacg gtcgatgtgg cgctgtcgcc cgacgatgcg ttcggcgagc
     6601 aggatccgga gttggtccgt attgaagatg cgggcgtgtt ccctgttgaa gtcgaagtcg
     6661 gcatgatgtt tgaagccgac gatcctgaaa ccggcgatgt tgtcgtctat cgtgtaaccg
     6721 atgttgccga cggcaaggcg gtggtggacg gcaaccatcc tttggcaggc atgaaaatcc
     6781 gctttaaggc tacggttgaa agcgtgcgcg atgcatccga tgaggaaatc gcacacggtc
     6841 atgtccacgg cccgcacggt catcaccacc actgattgcc ggcagcttgg aggtatctga
     6901 tgccgtctga aaagggtttg ttccgtttca gacggcattt tgtttgatgg atggagaagg
     6961 tatgtcggtt gggcattatg agaattttcc cgtcggttcg ctgattttgc cgcgcaggtt
     7021 gaggaagccg gttcacgcgg tgtacgcgtt tgcacggacg gcggacgata tggcggacga
     7081 gggcagtatg ccgtctgaag ccaggttggc ggggttggag ggtttgcagc gcgagttgga
     7141 cgtgttggca tcgggcggcc ggtcggcgca tcctttgatt gcacgattgg atgccgaggc
     7201 ggttgtgccg ttcggtttgg atttgcagcc gttttatgat ttgctttctg cattttctca
     7261 ggatgtggtt aaaacgcggt acgcgcattt cggcgagctg gccgattatt gtcggcgttc
     7321 cgccaaccct gtcggacgca ttatgctggc tttgtacggg aaaacggatg cggtgtgcgt
     7381 ggcgcaaagc gacggtatct gtacggcttt gcaactggtg aatttttggc aggatgtggc
     7441 tgtggattgg caaaagggca gggtttatat cccgcaggac gatttgttga aattcggtgt
     7501 ttccgaagga cagattgcgg agggtagggc ggatgcggcg tttcagcggc tgatggcgta
     7561 tgagtgccgg cgcgcattcc gtatgctgaa ggcgggttcg cctttggcgc gcgaattgaa
     7621 agggcgtatc ggtttggaac tccgtatgat tgtgttgggg gcgcagttga ttttgcagaa
     7681 actggacgcg tgccgatacg atgtgtttgc acaacgtccg gttttggata agaaggattg
     7741 gttgattatg ttgaaacgcg cgttgtggaa atgatggggg aaatgaatgc cgtctgaaag
     7801 gggttgttcc gcttcagacg gcattttgca ggcggttggt atggtcaggc gattttatgc
     7861 gggttcaacc tggcttgatc ttctgcaccc aagccgatga gtttgcgcag tcgggtcatg
     7921 tagaaattga catccaaacc ggtgccgtag cgttgcgccg tccacagtgt ttccgccagt
     7981 gcctccatca tttcgtgttc ggcttccagc cagccgcgtt tggcgcacag ggtgtcgtgg
     8041 attgcgcgta tgccgtgcgg ctggtctatg cccgcctgtt cttggacgga caaatgcagc
     8101 gacatatgca ggaaggggtt gctttcgccg ttttcgggca gccagtcggt gtccagatgg
     8161 tcttcgatgc gttcgagata acggtggtat tcgggatggg cttcgataat gcggagggct
     8221 ttctgttcca gtgcgctcag ttgcagcgga ttgagccgct gctgccacac gcgggcgaaa
     8281 aagcggcgga catcgtgggt attgacatcg tacatggcgg gttcggtaag atgtttatgg
     8341 aaataggttc ggataatggc gggttttaca taaattaaat cccaccgtca accttaccat
     8401 ttataatgca gacaaattat ttgtctgaaa cggattcgat tatgaaaaaa ctgatttgcc
     8461 tgttcgccgt atttttgatg ttgtgcggac gagctttcgc gctggatgcg aacgatctgc
     8521 tgccgccgga aaaggcattc gtgccggagc ttgccgttgc cgacgacggt gtgaacgtcc
     8581 gtttcaggat tgccgacgga tactatatgt atcaggcgaa aatcgtcggc aagaccaatc
     8641 cggcggattt gttgggacag ccttctttca gcaagggcga agagaaggaa gacgagtttt
     8701 tcggcaggca gacggtttac catcacgaag cgcaggttgc ctttccttat gcaaaggctg
     8761 tcggcgaacc gtataaattg gttttgacct atcagggctg tgccgaagtc ggcgtgtgct
     8821 atccgcccgt ggataccgag tttgatattt ccggcaacgg cacttaccat ccgcaaaccg
     8881 acgaaccggc atccgccaaa gaccgctttt tgcagccttc ctctcaaaac ggcagcgggg
     8941 cgctgccgcc cccgaagggg gatgagggcg gcgacagccg tttcaagctg tcttgggata
     9001 cgctcaacgc caatcttttg gcgttttttc tcgccggttt gggtctgagt tttaccgcct
     9061 gtatgtatcc cctgttgccg attgtttcca gtattgtggt cggcgacaaa aaggcgggca
     9121 aggcgcgggc gtttgtgctg tccgtcgttt atgttcaggg tttggctctg acttatacgc
     9181 tggtcggcat tgttgccgga ctgacgggcg cgctgctgac cgtatggttg cagcaggctt
     9241 gggtcgtgtt ggcggcatcg gctttgatgg tcgtcttggc actgtctatg ttcgggctgt
     9301 tcaacatcca gcttcccaac gccgtgcagt cgtattttca gaatcaaagc agcaggcttt
     9361 caggcggtaa aatcgtttcc gtctttatta tgggcatatt gtccgcgctg attgtcgggc
     9421 cgtgcgtcgc cccgccgctg gcatttgctt tgggctacat cggtcagacg ggcgatgcgg
     9481 ttttaggcgg tttggcactt tacactttgg cgttgggcac cggcgttccg ctgattgcca
     9541 tcggcacgtt cggcgggcat atcctgccta aggcaggcga ttggatgaat gccgtcaaat
     9601 acgctttcgg ctttatcctg cttgccgtcg ccgtttacct cgccacgccg cacttgccct
     9661 attatctcgt cgtcgcgctg tacacgctgc tgatgctggt tcctgcctgt atgctgctgg
     9721 tcaacggacg caggcagaaa cgccgtccga aagctgtggc attcgcattg ggcggcatat
     9781 tgctgatagg cggcgcgtgg ttcggctggc agggcgcaaa cggcaaaacg accgcgctgc
     9841 accatttcct gaccctcaat ccgccggccg aagcaggcaa atcttcggaa cacggaaaaa
     9901 tgtttgccga tactgccgcg ctgaaggcag cgatggatac ggcgttgaaa gaacatcccg
     9961 acaaacccgt cgttttggat ttttatgccg actggtgcat ttcctgcaaa gaaatggcgg
    10021 cttacacgct caatcagccg gaagtgcatc aggcagtcga tatggaacgc tttttccaaa
    10081 tcgacgtaac cgccaacaag cccgaacatc aggcgttgtt gaaagaatac ggtctgttcg
    10141 ggccgccggg cgtgtttgtc gtccgctccg acggcagccg cagcgagccg ctgctgggtt
    10201 ttgtcaaagc agacaagttt atcgagtggt atgaacaaaa ccgctgattc cgatgtttca
    10261 aatgccgtct gaagttctca gacggcattt ttgtcagaaa ggaaaacggc aatatccact
    10321 atacacacgg aagcagtcta aagatgtgaa aaccgtgtgt ataagttagt ttgcgtattg
    10381 aaattaaaag ataaaaatac gatgattaaa aaataggcat aagaaaagat aaacacagcc
    10441 ggtgtttgcc ggtgtgtgta tcttttgtgg aaaaacatct taatgtcatg tattttatgg
    10501 aaaataataa catgattaaa aaatagtcaa tca
//
LOCUS       DAWKST010000062        10235 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.062, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000062 DAWKST010000000
VERSION     DAWKST010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10235
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.062"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(324..1541)
                     /locus_tag="WJL87_001098"
     CDS             complement(324..1541)
                     /locus_tag="WJL87_001098"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ7799526.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVEAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLAAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1743..2471)
                     /gene="rph"
                     /locus_tag="WJL87_001099"
     CDS             complement(1743..2471)
                     /gene="rph"
                     /locus_tag="WJL87_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ7799527.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2553..3017)
                     /locus_tag="WJL87_001100"
     CDS             complement(2553..3017)
                     /locus_tag="WJL87_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ7799528.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3234..4010
                     /locus_tag="WJL87_001101"
     CDS             3234..4010
                     /locus_tag="WJL87_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ7799529.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERVAQINPGCQVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVYSTENIVPPQSREAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            4255..5109
                     /locus_tag="WJL87_001102"
     CDS             4255..5109
                     /locus_tag="WJL87_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ7799530.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFIALLELAKEGLVGIVQEVGFGEIRISLNHEGAHSDGIFG
                     TQGKCDVF"
     gene            5151..6359
                     /gene="pncB"
                     /locus_tag="WJL87_001103"
     CDS             5151..6359
                     /gene="pncB"
                     /locus_tag="WJL87_001103"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ7799531.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIKEDLEVELDALCQLRFTHDELDYLRSLRFIKSDFVDYLELFQLQRRFVEIGTD
                     DKDRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6451..8169
                     /gene="argS"
                     /locus_tag="WJL87_001104"
     CDS             6451..8169
                     /gene="argS"
                     /locus_tag="WJL87_001104"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ7799532.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIADSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGIAKTDRPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLAFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKARFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQAVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRFGTLHADRLLY
                     VVDHRQALHFEQLFTTSHKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKASEWDATAPTVLTEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAEGASRNSRLQLAKLTGDTLKQGLELLGI
                     DVLDVM"
     gene            8720..10138
                     /locus_tag="WJL87_001105"
     CDS             8720..10138
                     /locus_tag="WJL87_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ7799533.1"
                     /translation="MNIHALLSKQWTLPPFLPKRLLLSLLILLAPNAMFWVLALLTAT
                     ARPIVNLDYLPAALLITLPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVAAAGYFIGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
BASE COUNT         2359 a         3013 c         2578 g         2285 t
ORIGIN      
        1 cgcaacatta aatttctaga ttcccacttt cgtgggaatg acgggatgta tagtggatta
       61 acaaaaccag tacggcgttg cctcgcctta gctcaaagag aacgattctc taaggtgctg
      121 aagcaccaag tgaatcagtt ccgtactatt tgtactgtct gcggcttcgt cgccttgtcc
      181 tgatttttgt taatccacta taaatttaat ccactatatt ttttgttcca aagtcaaaat
      241 atgccgtccg aacattcggg cggcagacaa aacggcactg cccgataaag gcagtgccgt
      301 tgtccgtttc aaaccgtgaa acatcagccc aaattaaagg ctttatgcaa taccctggtt
      361 gccagttcca tgtatttttc atcaatcaat acggaaactt tgatttcgga ggtggaaatc
      421 atttggatgt tgataccctc ttcggcgagc gtgcggaaga ttttggcggc tacaccgacg
      481 tgcgaacgca tacccaaacc gactgcggag actttgcaca cggtgtcgtc gccgtcgata
      541 gaagccgcgc cgatactgtc ttgacgttcc gacaggattt ccaaagtctg cttgtaatcg
      601 ccgcgcggta cggtaaagga aaaatcggtt gtgccttcgc tgccgacatt ttggataatc
      661 atatcgactt cgatgttggc atcggcaacc gcgcctaaaa tctgataggc gacgccgggt
      721 ttgtcgggta cgccgcgcac gttgatgcgg gcttggtttt tgtcgaatgc gataccggtt
      781 acggcagctc tttccatatt gtcgtcctct tcaaaggtaa ttaaggtgcc gttgccgccg
      841 tcttgcaggc tgctcagtac gcgcaggcgc actttgtatt ttccggcaaa ttctactgaa
      901 cggatttgca aaactttcga gccaaggctg gccagctcaa tcatctcttc aaacgttacc
      961 gtatccatgc ggcgcgcttc gggtacgacg cgggggtcgg tggtgtaaac gccgtccacg
     1021 tcggtataga tttggcattc gtctgctttg agggctgcgg cgagcgcaac ggcggaggtg
     1081 tcggagccgc cgcgtccgag cgtggaaatg tcgccttcgc tgctgatgcc ttggaaaccg
     1141 gcgacgatga cgactttgcc ggcggcaagg tcggcacgca ttttttcgtc atcaatgctt
     1201 tcgatgcggg ctttggtgtg ggcggtatcg gttttgaggg cgacctgcca gccggtgtag
     1261 cttttggctt ccacgccgat gtctttcaat gccatcgcca aaaggccgat ggttacttgt
     1321 tcgccggtgg acaaaaccac gtccaattcg cgcggatcgg gatgctcctg catttcgtgc
     1381 gccaacgcga ccaggcggtt ggtttcaccg ctcatggcgg atacgacgac gacgatgtcg
     1441 tgtccttcgg cgcgggcttt ggcgacacgt ttggccacgt ttttaatgcg ttcgggcgag
     1501 cctacggatg tgccgccgta tttatgtacg attaacgcca tgtttcgtgc tttcttgtgt
     1561 gggattggtc gggcagcatg gtttgctgga aaagcggcta ttattactat tttttacatg
     1621 aagttcaaga acggactgcg ctttcccgcc tgccgtttga cagcggtcag cgaaaaacct
     1681 gttctttcag attgttgaca aaatgccgtc tgaacggttt tcagacggca tccggacgac
     1741 aatcaggcgg cggacaacgc attttgctgg tgttgcagca gttcgcctat gcctttttgc
     1801 gccagtgcaa ccagtttgcc caattcgtcc aaactgaacg gcgcgtcttc cgccgtacct
     1861 tggatttcga tgatttttcc cgatgcggtc atgacgatat tcacatcact gtcgcaaccg
     1921 gagtcttcgg gataatccaa atccaaaagc ggcacgccgt tcactacgcc tactgacaca
     1981 gcggcaacgg cttcgcggat ggggttttca ctcaaaatgc cgtctgaaac cagtttgccg
     2041 acggcgattt gcagcgcgac aaacgcaccg gtaatcgaag ccgtgcgcgt accgccatct
     2101 gcctgaatca catcgcagtc aatcaagatt tgtcgttcgc cgagtttttc catatctacg
     2161 accgcgcgta gcgaacgccc gataaggcgt tggatttctt gtgtgcgccc ggactgtttg
     2221 cccgccgaag cttcgcggcg catccgggaa gcagttgagg caggcagcat cccgtattcc
     2281 gccgttaccc agccttggtt tttaccgcgc agaaacggcg ggacgttttc atctatggaa
     2341 gcggtacaaa tcactttggt attgccgcat tcaataaggc acgaaccgtc cgtatgcggc
     2401 aggaaatgag gggtgatttt gatatcgcgc aggctgtccg cggcgcgcga gatgcggatg
     2461 taatcaggca tactgccctc ccgttaaaaa cagataaatt aaaaagcctt aaatatgaaa
     2521 aatcacattt aaggccttca aactgaaaat ttctacgcct cttcggcttt gctgcggata
     2581 atcaaaagcg gcaggtggct ttggcgcatt accgtttcgg caaaactgcc cattaaaagg
     2641 tgcatcagtc cggtacgtcc gtgcgtaccc aacaccagca ggtcggcacc gttttcatcg
     2701 gcataatcaa ccaaatcctg cgccatttca cgcgcaccct tattggcaac cagcaggtgt
     2761 ttgacggtat tttccacacc cagttcccgg gcggtgcgct cggcggcatc caaaacttcg
     2821 ttgccttgcg cgacggcggc ggcttcgtag ctttcgtgtt gcaaaaattc gggggcgagc
     2881 gccatatatt cggcaggatt ggcaacgtgt accaaagtca gacgcgcacc gttgaccccg
     2941 gcaagctcgg cggcatgttt cagggcattg atggacgttt cactgccgtc aacggcaaca
     3001 accagatgtt tgtacatatc gtattctcct tttgcaccgc ctcgcggtgc cctcttgtcg
     3061 gatgggcgca gggacagttt gcgccgtttc attatagacc cgctgtcggg ctttatacaa
     3121 cagccgaaca gcccgaccgc tttccagtat aatatgccgc ttccgtgcag tcaggcattt
     3181 tttgccggct ttcgttcact ttttgatttg acgcaatctt gcaggattcg accatgtccg
     3241 acaacgcttt gacctcttcg cgacgcttcg gcggcatcgc cagactctac ggagactctg
     3301 ccttggcgca cttttcacag gcacacgtct gcgtagtcgg cgtgggcggt gtcggctcgt
     3361 gggcggtcga ggctttggcg cggacgggca tcggacgttt gactttgatt gatttggaca
     3421 acgttgccga atcgaatgtc aaccgccagc tgcacgccct gaccggcgac ttcggcaaag
     3481 caaaagttac cgccttgcgc gaacgcgtcg cccaaatcaa cccaggatgc caagtgtttg
     3541 aaattgaaga tttcgttacc gaagacaatt tgccggaata cttcggaaaa ggttttgatt
     3601 tcgtcatcga cgcgatcgac caagtgcgcg tcaaagcagc aatggcggct tattttgtgg
     3661 aacgcaaaca accgtttgtc ctcagcggcg gcgcgggcgg acaaaaaaat ccggcgttaa
     3721 tccaaaccgc cgatttgagc cgcgtaaccc acgacccgct gcttgccaac ctgcgctaca
     3781 ccttgcggaa acgctacgga ttcagccgcg atacgaaagc aaatatgcgc gtgccttgcg
     3841 tgtattcgac cgaaaatatc gtgccgccgc agtctaggga ggcttgttcg gcagatgccg
     3901 ctccgcaagg cttgtcgtgc gccggctacg gtgcaagcat gctcgttacc gcttcgttcg
     3961 ggctatattg cgcacaggcg gcggtggaac acatcgcaga caaaaaataa gcaatgccgt
     4021 ctgaaacagg attcagacgg catttgaaca aacgcggcaa tcaagctgtc ggttcagaaa
     4081 aaccaacatc ggcacgccta aaaccgtatt cacaatctac tccttttcaa aacatttgca
     4141 tttaaaagcc gttataatgc cgtctgaaca tctgcccgac cacattatac gtgaatgtcg
     4201 gcagattgtt ttcttttgta aacttatatt aaaatccact taccgattca cgccatgccg
     4261 cccatccctg ccccatctgc accatccgag cacactgtcg catgggtatt cggccaaccc
     4321 gttaccgatt tgccccagga tttgtttatt ccgcccgatg cattgaaagt cgtattgggc
     4381 agcttccaag gccctttgga tctactgctg tatctgatcc gcaaacagaa tatcgacgta
     4441 ctggatattc cgatggtgaa gattaccgag cagtatctgc actacatcgc ccaaatagaa
     4501 acctatcagt ttgatttggc ggcggaatat cttttgatgg cagcaatgct gattgaaatc
     4561 aaatcgcgcc tgctgctgcc gcgtaccgaa accgtcgaag acgaagaagc cgacccgcgt
     4621 gccgagttgg tgcgccgcct gctggcttac gaacagatga agctggcggc gcagggtttg
     4681 gacgcgctgc cccgagccgg acgggatttc gcgtgggctt acctgccgct ggaaattgcc
     4741 gtcgaagcca agctgcccga agtctatatt accgacttga cgcaagcgtg gctgggtatt
     4801 ttgtctcggg caaaacacac gcgcagccac gaagtaatca aagaaaccat ctccgtgcgc
     4861 gcgcaaatga cggcaatcct gcgccgtttg aacggacacg ggatatgcag gtttcacgac
     4921 ctgttcaatc ccgaacaggg cgcggcttac gtggtcgtca acttcatcgc actgttggag
     4981 cttgccaaag aaggtttggt cggaatcgta caggaagtcg gtttcggaga aatccgaatc
     5041 agcctcaatc atgagggggc acattcagac ggcattttcg gtacacaggg caaatgtgat
     5101 gtgttctaat acacccccaa gccgccacca aaaatcggga gacacgccat atgaccggca
     5161 tcatacattc gctgcttgac accgacctct acaaattcac tatgctgcaa gtagtcctgc
     5221 accagtttcc gcagacgcac agcctttacg aattccgatg ccgcaacgcc tcgaccgtct
     5281 atccgcttgc cgacatcaaa gaagacttgg aagtcgaact cgacgcgctc tgccaactac
     5341 gcttcaccca cgacgaactc gactacctcc gcagcctgcg tttcatcaaa agcgactttg
     5401 tcgattatct cgaactcttc cagctccaac gccgctttgt cgaaatcggc acagacgata
     5461 aagaccgtct gaacatccgc atcgaaggtc cgatgataca ggcgatgttt tttgaaatct
     5521 tcatcctcgc cattgtcaac gaactttact tccgccgcct ggaaacccct gcagtcatag
     5581 aagaaggcga acgccggctt caagccaaag ccgcgcgcct caaagaaatc gccgccgcac
     5641 aaaaccccga cgaaccgccc ttcctgattt ccgacttcgg cacgcgccgc cgctacaagc
     5701 tcgcgtggca ggaacacgtc atccgcaccc tgctcgaagc cgccccaagc atcgtgcgcg
     5761 gcaccagcaa tgtctttctc gccaaaaaac tcggcatcac ccccatcggc accatggcgc
     5821 acgagttcct gcaggcattc caggccctcg acgtacgcct gcggaatttc caaaaggccg
     5881 cgctcgaaag ctgggtgcac gaataccggg gcgatttggg cgttgccctg accgacgtgg
     5941 tcggtatgga tgccttcctg cgcgatttcg acctctattt cgccaaactc ttcgacgggc
     6001 tgcgccacga cagcggcgac ccttacgttt ggggcgacaa agcctacgcc cactatcaaa
     6061 agctcaaaat cgacagccgc accaaaatgc tgactttctc cgacgggctg gacatcgaac
     6121 gctcttgggc attgcaccaa tatttcaaag accgcttcaa aaccggcttc ggcatcggca
     6181 ccaacctcac caacgatatg gggcatacac ccttgaatat cgtcttgaaa ctggtcgaat
     6241 gcaacgggca gtccgtcgcc aagctgtccg actctccggg caaaaccatg accaacaaca
     6301 gcaccttcct cgcctacctg cgccaagtgt tcgacgtacc cgaacccgaa acgccgtaaa
     6361 ccggcagaaa aagcgcacaa ttcctgtttc tgccgcataa aatcttttaa aataccgcct
     6421 gatttgaatt taaccgaaag accgaacttc atgaacctac atcaaaccgt cgaacacgaa
     6481 gccgccgccg cctttgccgc cgcaggcatc gccgacagtc ccgttgtttt gcagccgacc
     6541 aaaaatgccg aacacggcga tttccaaatc aacggcgtga tgggtgcggc gaaaaaagcc
     6601 aaacaaaatc cgcgcgaact ggcgcaaaag gtcgccgacg cattggcggg caacgccgtc
     6661 atcgaaagcg cggaagtcgc cggtccgggc tttatcaacc tgcgcctgcg ccccgaattt
     6721 ctcgcgcaaa acattcagac ggccttgaac gacgcgcgtt tcggcatagc gaaaaccgac
     6781 aggccgcaaa ccgtcgtcat cgactattcc tcacccaatc tggcaaagga aatgcacgtc
     6841 ggccacctgc gttccagcat catcggcgac agcatttcgc gcgtgttggc atttatgggc
     6901 aacaccgtca tccgccaaaa tcacgtcggc gactggggta cgcagttcgg catgttggtc
     6961 gcttatttgg tcgagcagca aaaagacaat gccgcgttcg agctggcgga tttggagcag
     7021 ttttaccgcg ccgccaaagc gcgctttgac gaagaccctg cctttgccga caccgcacgc
     7081 gaatacgttg tgaagctgca aggcggcgat gaaaccgttt tggcgttgtg gaaacagttt
     7141 gtcgatattt cgctctcaca cgcccaagcc gtttacgaca cgctgggctt gaagctgcgc
     7201 cccgaagacg tggcaggcga atcgaagtac aacgacgatt tgcaagccgt ggtcgatgac
     7261 ttggttcaaa aaggtctggc agtcgaagac gacggcgcga aagtcgtatt cttggacgag
     7321 tttaaaaaca aagagggcga acctgccgca tttatcgtgc aaaaacaagg cggcggcttc
     7381 ctttatgctt ccaccgactt ggcatgtttg cgctaccgtt tcggcacgct tcatgccgac
     7441 cgcctgctgt acgtcgtcga ccaccgccaa gccctgcact tcgagcaact ctttaccact
     7501 tcccacaaag caggctatct gccggaaaac gtcggcgcgg catttgtcgg cttcggcacg
     7561 atgatgggca aagacggcaa accgttcaaa acgcgcagcg gcgacaccgt aaaactggtc
     7621 gatctgctga ccgaagccgt cgaacgcgcc accgctttgg tgaaagaaaa aaatcccgaa
     7681 ttgggcgcgg atgaagccgc caaaatcggc aagaccgtcg gcatcggcgc ggtcaaatac
     7741 gccgatttga gcaaaaaccg caccagcgat tatgtgttcg actgggacgc catgctctcg
     7801 tttgaaggca acaccgcccc ctacctgcaa tacgcctaca cccgcgtgca aagcgtgttc
     7861 cgcaaagcaa gcgaatggga cgcaactgcg ccaaccgttt tgaccgaacc tttggaaaaa
     7921 cagcttgccg ccgagctgct gaaattcgaa gacgtgctgc aaagcgtggc ggacacggcg
     7981 tatccgcact acctcgccgc ctacctctat caaattgcca ccctgttcag ccgcttctac
     8041 gaagcctgcc cgattttgaa agccgaaggc gcaagccgga acagccgcct gcaactggcg
     8101 aagctcaccg gcgacacgct gaaacaaggc ttggaattgt taggcatcga tgtgttggac
     8161 gtaatgtaaa accgcaccgc ccgattgcgg acaacagccc tgccatcctt atccgaatct
     8221 gaaaaaagcg gcgcgataca ccgtatccgc cgcccctccc aaaatgcgaa acagacaaac
     8281 gccaagcaag caagcaagca agcaagcaag caagcaagca agcaagcaaa aaattataac
     8341 ccccttcctg ccgacgcacg cactttccgc gcggcgcatt cccctttttc cgcccctcaa
     8401 acccgccttt tcttcaggca gggtttcagc ccgccccctt tccctgtttt cctttccccg
     8461 acacgcgcgc gcgacccctg ccgcatcgtg ccgcactttc gcgcccggac ggcatcgttc
     8521 cgccatccgg ttctctgttt caaatacccc tgtttcagaa agaaattcta gatgcttcaa
     8581 cacacaggac gacatataaa gcgtcgccct atatgttgcc ctgatttgga aggggttacg
     8641 cctcccaaat aaagcctgat cctgccgccc cgaaggacag atgtccgagt ggcggggttt
     8701 caaccgaaaa ggaaatacga tgaatattca cgccctgctc tccaaacaat ggacgctgcc
     8761 gccattcctg ccgaaacggc tgctgctgtc cctgctgata ctgctggccc ccaatgcgat
     8821 gttttgggtt ttggcactgc tgaccgccac cgcccgcccg attgtcaatt tggactacct
     8881 tcccgccgcg ctgctgatca ccctgccttg gcgtttcgtc aaaattgccg gcgtattggc
     8941 gttttggctg gcagttttgt ttgacgggct gatgatggtg atccagcttt tcccttttat
     9001 ggacctcatc ggtgccatca acctcgtccc cttcatcctg accgcccccg ccccttatca
     9061 gataatgacc gggctgttgc tgctgtatat gctggcgatg ccgtttgtgt tgcagaaagc
     9121 cgccgccaaa accgacttcc ggcacattgc cgtctgcgcc gccgttgtgg cggcagccgg
     9181 ctatttcatc ggccatttga gttactacga ccggggtcgg atggccaata tcttcggcgc
     9241 aaacaacttc tactacgcca aaagtcaggc gatgctctac accgtcagcc agaatgccga
     9301 ctttattacc gccggcctgg tcgatcccgt cttcctcccc ttgggcaatc aacagcgtgc
     9361 cgccacgcat ctgaacgagc cgaaatctca aaaaatcctc tttatcgtcg ccgaatcttg
     9421 ggggctgccg gccaatcccg aacttcaaaa cgccactttt gccaaactgc tggcgcaaaa
     9481 agaccgtttt tcggtttggg aaagcggcag ttttcccttt atcggcgcga cggtcgaagg
     9541 cgaaatgcgc gaactgtgcg cctacggcgg tttgcgcggg ttcgcactgc gccgcgcgcc
     9601 cgacgaaaaa tttgcccgct gcctccccaa ccgtttgaaa caagaaggtt acgccacctt
     9661 tgcgatgcac ggcgcgggca gttcgcttta cgaccgcttc agctggtatc cgagggcggg
     9721 ctttcaagaa atcaaaaccg ccgaaaacct gatcggtaaa aaaacctgcg ccattttcgg
     9781 cggcgtgtgc gacagcgagc tgttcggcga agtgtcggca tttttcaaaa aacacgacaa
     9841 gggactgttt tactggatga cgctgaccag ccacgccgac tatcccgaat ccgacatttt
     9901 caaccacagg ctcaaatgca ccgaatacgg cctgcccgcc gaaaccgacc tctgccgcaa
     9961 cttcagcctg cacacccaat tcttcgacca actggcggat ttgatccaac gccccgaaat
    10021 gaaaggcacg gaagtcatca tcgtcggcga ccatccgccg cccgtcggca acctcaatga
    10081 aaccttccgc tacctcaaac aggggcacgt cgcctggctg cacttcaaaa tcaaataaca
    10141 acaatgccgt ctgaacgcac caacagcctt cagacggcat tttgcagaca gaccgaccct
    10201 tcaagcccac ttttttcatc atctccgata aattg
//
LOCUS       DAWKST010000063        10180 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.063, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000063 DAWKST010000000
VERSION     DAWKST010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10180
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.063"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..1488)
                     /locus_tag="WJL87_001106"
     CDS             complement(<1..1488)
                     /locus_tag="WJL87_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ7799534.1"
                     /translation="MNKTLKRQVFRHTALYAAILMFSHTGGGGAQAETHNYAIVMNEQ
                     NQLKVKQKGSYSTLREKDRERKFIYNKSWQGGGSVFFDNTDTLVSRQSGTAVFGTATY
                     LPPYGKVSGFDANALKERNNAVDWIHTTRAGLAGYAYTNVVCRSHQCPQLVYKTRFSF
                     DNPDLAKRGGGLDRHTEPSRDNSLIYKLKDHPWLGVSFNLGSENTVKNSQSSSRLISS
                     FSEDNNNQTIVSTTENHPISLGDRQREHTAVAYYLNAKLHLLDKKGIKDITGKTVRLG
                     VLKPSIDVKTQRTGLGGLLGFHAKWDIKDTGQIPVELGLPQIKAGRCINKPNPNPKAQ
                     ALSPALTAPALWFGPVQNGKVQMYSASVSTYPDSSSSQIFLQNLSRKDDTSKPGRYSL
                     KPLSTSEIKSKEPTFTGRQTVIRLDGGVQQIKLQGNEVTSFNVNNDNNTFGIVKDLGV
                     DPDASEWKKVLLPWTVRASNDDNQFKTINQQLNQQKIQYSQRYRIR"
     gene            complement(2125..3204)
                     /gene="apbC"
                     /locus_tag="WJL87_001107"
     CDS             complement(2125..3204)
                     /gene="apbC"
                     /locus_tag="WJL87_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ7799535.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIAAAVADSVQEILMPETGGAHIHLSIDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGATAQLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
     gene            3415..3490
                     /locus_tag="WJL87_001108"
     tRNA            3415..3490
                     /locus_tag="WJL87_001108"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3448..3450,aa:Lys,seq:ttt)
     gene            3569..4078
                     /locus_tag="WJL87_001109"
     CDS             3569..4078
                     /locus_tag="WJL87_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ7799536.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWIRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSSA
                     EVAAYMAKRGLGFPTANDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            4421..4861
                     /gene="nadR"
                     /locus_tag="WJL87_001110"
     CDS             4421..4861
                     /gene="nadR"
                     /locus_tag="WJL87_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ7799537.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            4976..5476
                     /gene="hpaC"
                     /locus_tag="WJL87_001111"
     CDS             4976..5476
                     /gene="hpaC"
                     /locus_tag="WJL87_001111"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ7799538.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVTP
                     VTDEPPTVMLCINRKARIIPILSENGSLCINTLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRFLD"
     gene            5492..6187
                     /locus_tag="WJL87_001112"
     CDS             5492..6187
                     /locus_tag="WJL87_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ7799539.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVVNGDVLTDIDFTAAFQAASSLPEHISAHLWLVENPPHNPDGDFSLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(6418..6849)
                     /locus_tag="WJL87_001113"
     CDS             complement(6418..6849)
                     /locus_tag="WJL87_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ7799540.1"
                     /translation="MSSNAWLFWALASAGFASLTAVFAKMGLQGIDSDFATFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGRNWTFLILSGLATGASWLAYFKALQLGNASQVAPID
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            7024..8700
                     /locus_tag="WJL87_001114"
     CDS             7024..8700
                     /locus_tag="WJL87_001114"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ7799541.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELDIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKAHGAMAVLLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLADLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGVGGADLARKVVNAIENQPNNFNFSYDVELSIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RITVRGITVSSGAGFIVALCGNMMKMPGLPKVPAAEKIDVDAEGVIHGLF"
     gene            8814..9905
                     /gene="ychF"
                     /locus_tag="WJL87_001115"
     CDS             8814..9905
                     /gene="ychF"
                     /locus_tag="WJL87_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ7799542.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LERLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
BASE COUNT         2252 a         2584 c         2897 g         2447 t
ORIGIN      
        1 gcggatgcgg tatctttggc tgtattggat tttttgttgg ttaagttgtt ggttaattgt
       61 tttaaattga ttgtcatcat tggaagcacg aaccgtccaa ggcagcagca cttttttcca
      121 ctcgctggca tcaggatcga cgcccaagtc cttaacaatg ccgaaagtgt tgttgtcgtt
      181 gtttacatta aaactagtga cctcattgcc ttgaagttta atctgctgta cgccgccatc
      241 caatcggatg acggtttgcc gccctgtgaa agtcggctct ttacttttaa tctccgacgt
      301 actcaagggt ttgagggaat agcggcccgg tttgcttgtg tcatccttgc gggaaaggtt
      361 ttgcaggaaa atttggctgc tcgaactgtc ggggtaggtg gaaaccgaag cggaatacat
      421 ctgcaccttg ccattttgca caggtccgaa ccacagcgcg ggggcagtca gtgccggcga
      481 aagggcttgg gctttgggat tggggttcgg tttgttgatg cagcggcctg ctttgatttg
      541 cggcaggccg agctcgaccg gaatctgccc ggtatcttta atgtcccact tagcatgaaa
      601 acctagcaag ccgccaagcc ccgtcctttg tgtcttcaca tcgatgctcg gcttcaagac
      661 acccaaccgc actgttttgc cggtgatatc tttaatccct tttttatcca gcaggtgcag
      721 tttggcgttc agataatagg ccacggcggt atgttcgcgc tgcctgtcgc caagggaaat
      781 agggtggttt tctgtcgtag agacgatggt ttgattatta ttgtcttcac taaaagaaga
      841 tatcaatctg ctggatgatt ggctattttt gacggtattc tcgctgccca aattgaaaga
      901 tacgcccaac catggatgat ccttcaattt gtaaatgagc gaattgtcgc ggcttggctc
      961 tgtgtgccta tccagcccgc ctcctctttt tgccaagtcg ggattatcga aggaaaatcg
     1021 ggttttatag acaagttggg ggcattggtg gcttctgcat acgacgttgg tgtaggcgta
     1081 gcctgccagc ccggcccggg tggtatgaat ccaatcgacg gcattgttgc gctctttcag
     1141 agcgttggca tcaaaaccgg aaaccttgcc gtagggcggc aggtaggtgg ctgtgccaaa
     1201 aacggcagta ccgctttgtc gggaaacaag ggtatcggta ttgtcgaaaa agacagagcc
     1261 tccaccctgc cagcttttat tatagataaa tttgcgttcc ctgtcttttt cccttaaggt
     1321 tgaatatgaa cccttctgct ttaccttcag ctgattttgt tcgttcatga caatagcgta
     1381 gttatgggtt tccgcctgcg cccccccccc cccggtatgg gaaaacatca agatggcggc
     1441 ataaagcgcg gtatggcgga aaacctgcct ttttaaagtt ttattcatcg tatttccttt
     1501 tcggtcaaac cccccgccct tcgggcatcc gtccttcggg gcggcaggat caggctttgt
     1561 ccgggagggg tgcaagccct tccgaatcag ggcggcgcct tatgtgccgc cctgcgtgtt
     1621 ggaacatatg tttcgcaggg tgtttcgtgg gtttcgcagg gcgggctgta gggcgggctt
     1681 tagcccactc tatggcgcac cttgcgaatc tgccttataa aaatacccga accgtcattc
     1741 ccgcgcaggc gggaatctag gatgcggaat ctagagaaac cgtttatccg ataagtttcc
     1801 gttccgacag gtctggattc ccgcctgcgc gggaatgacg gacgggaacg gcgcacgggc
     1861 attgccggaa tcttttgtgt ttccattatc atactctttt taatgcgcta ttccaagaat
     1921 atgatgccct ccggcggcat cccaccctac ggcttgctat aaaaatacct aaaccgtcat
     1981 tcccgcaaac ctacattccg tcattcccac gaaagtggga atccggaacg taaaatctca
     2041 aaaaaaccgt tttatccggc aagtttgccc gccggcaggc gcggggcttg ggacggcatt
     2101 gctgttgcgg tttcggacgc ggctttattc gacgacaatt ttggggaagc ggctgctgaa
     2161 gtctttgcct ttgtcggcaa tgcccagggc gatttggaat gcggcatcgg tgtagattcg
     2221 ggcgatggcg gggtgttcgt cgaacagttg cgccgtcgcc cctccgtcca tggcttcgcg
     2281 cacgggcagg cttaatggaa gctgtcccag cagggggacg ttgaggcgtg ccgccaagtc
     2341 tttgccgccg tctgtgccga acagtgcttc gctgtgtccg cagttggagc agatgtggac
     2401 ggacatattt tccaatacac ctaaaatggg aatgttgact tttcggaaca tatccacggc
     2461 tttgcgcgcg tctatcaggg cgatgtcctg cggcgtggtt acgatgaccg atccggttac
     2521 ggggatgcgt tgggacagcg tgagttggat gtcgcccgtg ccggggggca ggtcgataaa
     2581 caggtagtcc acttcgtccc actcgctttg aaacatcagt tgctgcaagg cttggctgac
     2641 catcggcccg cgccagacga cggcttggtc ggtatcgacg agaaagccga tagacatgac
     2701 ctgtatgccg tctgaagatt cgacgggaat gagtttttgg tttttctgat cgggtttgcg
     2761 gtcgtccaca cccaacatgg tcggttggct cgggccgtaa aggtcggcat cgagcacgcc
     2821 gacgcgcgcg cccatccgcg ccattgcggc ggcaaggttg gcggtggttg ttgatttgcc
     2881 cacgccgcct ttgccggagg cgacggcaat gatgtttttc acacctttga tggtggtaac
     2941 gccaggttgg actttatgtg tgccgatttc ggtgtctatg gacagatgga tgtgcgcgcc
     3001 gcctgtttcg ggcatcagga tttcttgtac ggaatcggcg acggcggcgg cgatgtgcgc
     3061 gacagggaag ccgaaatgca gggcgatatg gatgccgtct gaacgctgtt cgaccgaacg
     3121 gacggccttt tcgccgccga gcgtgcgtgc cgtattcgga acggcgacgg tgtcgaggag
     3181 ggtgcggatg ttttgtatgt tcataatgtg tgtttctgtg tgcagtttgg gaaacgggcg
     3241 gaaagtgtac attgatttcg ggcggtttgc ttttccttgt gcgaactttt acacggtatg
     3301 cggcggattg tgaaaatccg ttgtcaaaat atcccgtgca aacagtggtc tgcaattaag
     3361 cctgtaaaat ttgcttgacg aggtaggatg gtgctgtata atgcacggct tatcgggtcg
     3421 ttagctcagc cggtagagca gcggactttt aatccgttgg tcgagcgttc gaatcgctca
     3481 cgacccacca gataaccgga agccaagttt tgagacttgg ctttttgttt gcctgccggt
     3541 tgcgtgggcg taccgtttga gagagcgtat gaaaaagaaa ctgctttcgg gcatcaaatt
     3601 tgccgttcag acggcattgg tgtttttatt ggtgtcgctg tttttggatt ggatacgcaa
     3661 gcccgacgaa cctgccggtg cggcagggcg gcctttgacc ctgctgtcgg ggcagcggct
     3721 gactttggga cagtttagcc gagataaggc ggttttggtg tatttttggg ggagctggtg
     3781 cggcgtgtgc cgttatcagt cgccgataat cgatgatttg gcggcggacg gcgtgccggt
     3841 cgtcggcgtg gcggtacgtt ccggcagttc ggcggaagtg gcggcgtata tggcaaaacg
     3901 cggcttgggc tttccgactg ccaacgatga ggacgggggt ttggcaaggt cttggcggat
     3961 tgtcgcaacg cctgccgtcg ttttggtcaa aaatgggaaa atggtccatt atacgacggg
     4021 aatcagcagt tattggggct tgcgcgcacg aatttttcag gcggatgttt tcggttaact
     4081 tttgtttttg ttaaacttta cgctatgtag agaatcaaac ggcatatacc ttgctcgtct
     4141 gatgcggcgg cttttgtttt ttctggaaga aagttctttc gacagtaaat gtaatacttt
     4201 gttgagattg tagggaaaat agactaagat ataactatta aaatattttt agataggatt
     4261 atcggaatta aagtctttta tacccagtcg tccgatgcgg tttatagcgt attgttgcta
     4321 tatgttcgtt ttgttatata acggttgcat caaaatttac gcccacaggc tttcccgacg
     4381 gtttgaaagt ttgattttcg ataacttgga gacttgaaca atgcctaccc aatcaaaaca
     4441 tgcgtctatc aatatcagtc tgatacaggc aagggaagcc ctgatgaccc aattcaggcc
     4501 tattctgaat caggcgaata ttaccgatca gcaatggcgg attatccgtc ttttggcgga
     4561 aaacggcacg ctggactttc aagatttggc gaatcaggcg tgtattctgc gccccagcct
     4621 gaccggtatc ctgacccgcc ttgaaaaagc gggtttggtt gtccgcctga aaccttccaa
     4681 cgaccaacga cgtgtttttc tgaagctgac tgccgagggc gagaagctgt atgaggaaat
     4741 cggcgaagaa gtggacgagc gttacgacgc tatcgaggaa gtgctgggcc gcgagaaaat
     4801 gctgctgctt aaagacctgt tggcagaact tgccaaaatc gaggatgcgt tgaactcgta
     4861 atacgccgta acgcgcggaa acgtccgacc gacggctttt tgaatcagaa ctgctgcaca
     4921 tggggggtgc cttgtgtgca gcattcttat ataggggaca gtttaaaggg gaaaaatggc
     4981 ggatttgcag aaaacttttc aaacttcgtt ccgtgatgcg atggcatcct gtgcggcagg
     5041 cgttcatgtc atcacgacag acggtgcggc agggcgttac ggcattacaa tgacggcggt
     5101 tacaccggtt acggacgagc cgccgaccgt gatgctgtgc atcaaccgta aagcccgtat
     5161 catcccgata ctgtcggaaa acggcagcct gtgcatcaat acgttggcag acgaacatca
     5221 ggatgttgcc gaacattttg ccgggctgac cgggctgtcg cccgaagagc ggtttgccta
     5281 ccacatctgg catcgcggca aaacgggaca acttgaaata gagggcgcgt tggcgcacct
     5341 gcacgggcat attgtcggca aacatgaaat cggcacgcat tttgtgtttt acgtcaggct
     5401 cgacgaaatc aaaaactgcg ggtgcaaacg ccccgcgctg ctgtatttca gacggcagtt
     5461 tagattttta gactgatatt cggacagata tatgaaagcg atgatactgg cggcaggacg
     5521 cggcgagcgt atgcgccctt tgaccgatac cactccgaag ccgctgctcg atgtggcggg
     5581 taagcctcta atcggttggc acctatgccg tctgaagcag gcggggttta ccgaaatcgt
     5641 catcaaccac gcttggctgg gccggcagat agaagatgct ttgggcgacg gctcggctta
     5701 tggcgtgaac atcgcctatt cgcccgaacc cgcaggcggt ttggaaacgg caggcggcat
     5761 cgcgcaggca ttgccgctgt tgggtgggca gccgtttttg gtggtcaacg gcgacgtgct
     5821 gaccgacatc gattttaccg ccgcgtttca ggcggcatcg tccctgccgg aacatatttc
     5881 cgcccatctg tggctggtgg aaaatccgcc gcacaacccc gacggcgatt tttccctgct
     5941 gcccgacggc agcgtgcggc cggaagtaaa tggcggcaac ggattgacat tcagcggcgt
     6001 gggtatttac cgtcctgaaa tgtttgacgg aatcgaagcg ggcagtgtgg cgaaactcgc
     6061 gcccgtattg cgtggcgaaa tgcggcaaaa ccgcgtgagc ggtcagaagc atacgggctt
     6121 gtggctggat gtcggcacgg tatgccgtct gaaagaggct caagcccttg caggggcttg
     6181 gaagtgaaat aaaacggtgg cttgccgagc cggctgccga acggttccga ccggatgtcc
     6241 agcatatttt cgtggaggcg tgaaaatagg gcgttcgggg ctttccgtcc aacaaatgcc
     6301 atctgaagcg cggcggtttg ccgtcggcgc ggtgtcggat tttattgggt aactttgttc
     6361 agaccgccgc ccgtcagatt gtcaaaaccc ggtttcaggc ggtatggcgg attcggttta
     6421 acgtttcaac gccagcacca acacgcccgc cgttaccagc cccaagccta tccattcctg
     6481 cgtgttcggg cgttcgtcca agaaaaccac cgccatcagc gcgaccaaga ccaggctgaa
     6541 tttgtcgatg ggggcgactt gcgaggcgtt gcccagttgc agggctttga agtaggcgag
     6601 ccaggatgcg ccggtagcaa ggccggatag gataaggaat gtccagttgc gccccgtaaa
     6661 gccgttcaca ccctgccatt tgccggtgta ggtcaaaaac aataccaaag cggcgaggat
     6721 caccaaggtg cggataaagg tggcgaaatc ggaatcgatg ccctgtaaac ccattttggc
     6781 gaaaacggcg gtcaatgagg cgaagcctgc cgatgccaat gcccaaaaca gccatgcgtt
     6841 gctgctcatg ttttctcctt ggattgtgaa caatatgaac ggtatttttg ttgcagcgtc
     6901 aaaaatttca ctgcgggttt ggtgcggata acgttataat atgcctgata ttattttcaa
     6961 tccacctgtt tgtcgcctga tgctttcaga cggcatgtcc ctcctcattt ctaaaggaaa
     7021 atcatgagct tcaaaaccga tgccgaaatc gcccaatcct ccaccatgcg cccgattggc
     7081 gaaattgccg ccaagctggg tctgaatgcc gacaacattg agccttacgg tcattacaag
     7141 gcgaaaatca atcctgccga agcgttcaaa ctgccgcaaa aacagggcag gctgattttg
     7201 gttaccgcca ttaatccgac tccggcgggc gaaggcaaaa ccaccgtaac catcggtttg
     7261 gcggacgcgt tgcgccacat cggcaaagat gccgtgattg ccctgcgcga accttctctg
     7321 gggccggtgt tcggcgtgaa aggcggcgcg gcaggcggcg gctatgccca agttttgccg
     7381 atggaagaca tcaacctgca cttcaccgga gattttcacg ccatcggtgc ggcaaataat
     7441 ctgcttgccg cgatgctcga caaccatatc tatcagggca acgagttgga catcgacccc
     7501 aaacgcgtgc tgtggcggcg cgtggtcgat atgaacgacc gccagttgcg caacatcatc
     7561 gatggcatgg gtaaacccgt tgacggtgtg atgcgccccg acggttttga tatcaccgtt
     7621 gcctccgaag tgatggcggt attctgcctt gccaaagaca tcagcgattt gaaagagcgt
     7681 ttgggcaaca tcctcgtcgc ctacgccaaa gacggcagcc ccgtttacgc caaagatttg
     7741 aaggcacacg gcgcgatggc ggtcctgctc aaagatgcga ttaagcccaa tttggtgcaa
     7801 accatcgaag gcacgcccgc cttcgtacac ggcggcccgt tcgccaacat cgcccacggc
     7861 tgcaactccg taaccgcaac ccgtctggcg aaacaccttg ccgattacgc cgtaaccgaa
     7921 gcaggcttcg gcgcggattt gggcgcggaa aaattctgcg acatcaaatg ccgccttgcc
     7981 gatttgaaac ctgatgcggc ggtcgtcgtg gcgactgtcc gcgctttgaa atacaacggc
     8041 ggcgtggaac gtgccaacct cggtgaagaa aacctcgaag ccttggcaaa aggtttgccc
     8101 aacctgctga aacacatttc caacctgaaa aacgtattcg gactgcccgt cgtcgttgcg
     8161 ctcaaccgct tcgtgtccga ctccgatgcc gagttggcga tgattgaaaa agcctgtgcc
     8221 gaacacggcg ttgaagtttc cttgaccgaa gtgtggggca aaggcggcgt gggcggtgca
     8281 gacttggcgc gcaaagtcgt caacgccatc gaaaaccaac cgaacaactt caatttttcc
     8341 tacgatgttg agctgagtat taaagacaaa atccgtgcga ttgcccaaaa agtgtacggc
     8401 gcggaagatg ttgatttcag cgcggaagcg tctgccgaaa tcgcctcgct ggaaaaactg
     8461 ggcttggaca aaatgccgat ttgcatggcg aaaacccaat attctttgag cgacaacgcc
     8521 aaactcttgg gctgccctga aggcttccgc attaccgtgc gcggcattac cgtttcttcc
     8581 ggtgctggtt tcatcgttgc gttgtgcggc aatatgatga aaatgccggg tctgcccaaa
     8641 gttcccgccg ccgagaaaat cgacgtggac gcagaaggcg tgattcacgg cttgttctga
     8701 acggtttttg aaaccagatg ccgtctgaag ccgtttcaga cggcattttt tcggaacgcg
     8761 ggcggcggta tgctataatc cgccgttaaa tttctctatt ttcaggaaaa aacatgagtt
     8821 tgaaatgcgg catcgtcggt ttgcccaacg tcggcaaatc cacccttttt aacgcgctga
     8881 cccaatcggg tatcgaagcg gcaaactatc ctttctgtac catcgaaccc aacgtcggca
     8941 tcgtcgaagt ccccgatccg cgtatggccg aattggcaaa aatcgtcaat ccgcaaaaaa
     9001 tgcagcctgc catcgtcgaa tttgtcgata ttgccggttt ggttgcaggc gcgagcaaag
     9061 gcgagggctt gggcaaccag ttccttgcca acatccgcga aaccgatgcg attgtgaatg
     9121 tcgtgcgctg ctttgacgac gacaacatcg tccacgttgc aggccgcgtc gatccgattg
     9181 ccgacatcga aaccatcggc acagagttgg cacttgccga cctggcaagt gtggaaaaag
     9241 ccatcgtccg cgaagaaaaa cgcgcccgtt caggcgacaa agacgcgcaa aagctggtcg
     9301 atttgtgcaa aaaactgctg ccgcatctgg acgaaggcaa acccgtgcgt tccttcggtt
     9361 tggacgcgga agaacgcgcg atgctcaaac cgctgttcct gctgaccgcc aaaccggcga
     9421 tgtatgtggg caacgtcgcc gaagacggtt ttgaaaacaa tccgcacctc gaacgcctga
     9481 aagaattggc ggcaaaagaa aacgcccccg tcgtcgccgt ttgcgccgca atggaaagcg
     9541 aaatcgccga attggaagac gacgaaaaag ccgagttcct cgccgaaatg ggcttggaag
     9601 aaccgggcct gaaccgcctg atccgtgccg gctacgacct tttggggctg caaacctact
     9661 ttaccgccgg cgtgaaagaa gtccgcgctt ggacgataca caaaggcgac accgccccgc
     9721 aagccgccgg cgtgattcac accgactttg agcgcggctt catccgcgcc caagtcattt
     9781 cttacgacga ctttgtctcg ctcggcggcg aagccaaagc caaagaagcc ggcaaaatgc
     9841 gtgtggaagg caaggaatac gtcgtgcagg atggggatgt gatgcacttt ttgtttaacg
     9901 tgtaacccaa atgcggcagg ttttcagacg gctttgccga aaatgccgtc tgaagtcggt
     9961 tttggtggct ttcggcgttt ccataccgcc ggaatgcggc tgcatcaaaa taaaatcccg
    10021 cccgcatttc cgatttgccc tccccgattc ctgcaaaaca aaccgcctgc cccgctgttg
    10081 caaaaagccg tccggcattc cgaatatccc gagccccgat acaaaatgac ctttcagacg
    10141 gcatttgcgc cgccgcgttt cgagtatagt ggattaactt
//
LOCUS       DAWKST010000064        10119 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.064, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000064 DAWKST010000000
VERSION     DAWKST010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10119
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.064"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(83..277)
                     /locus_tag="WJL87_001116"
     CDS             complement(83..277)
                     /locus_tag="WJL87_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ7799543.1"
                     /translation="MKHKLSSWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(267..2348)
                     /locus_tag="WJL87_001117"
     CDS             complement(267..2348)
                     /locus_tag="WJL87_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ7799544.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPALQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DSKQPEAQSEA"
     gene            2824..3204
                     /locus_tag="WJL87_001118"
     CDS             2824..3204
                     /locus_tag="WJL87_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ7799545.1"
                     /translation="MNIRFFALTVPVLSLAACAVPETYDGGGRGHMPPVQNQARPADF
                     RAFSCENGLSVRVRRLDGGRIALRLDGRRAVLSSDVAASGERYTAEHGLFGNGTEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3488..4114)
                     /locus_tag="WJL87_001119"
     CDS             complement(3488..4114)
                     /locus_tag="WJL87_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799546.1"
                     /translation="MEKIEKFKSELLDDIFQYIQCISIFVYKKKIYYLIDYKENFELN
                     AKISFDIYLREGIITKEQYNYDYKNYRNGIWQLTKDNFESYLQSDSVIVLKKDELKEL
                     MFQGFTSAEAVRLYSVVENKLSYNDPISDSGRQSDFLKINQISSRLPLFYVNFDTEVY
                     LHMDWDRCHEDYVYDGWFSKAMDFGYLIPDEFCYWKIEGRDYWKFGQL"
     gene            complement(4268..4759)
                     /locus_tag="WJL87_001120"
     CDS             complement(4268..4759)
                     /locus_tag="WJL87_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdiI family contact-dependent growth inhibition
                     immunity protein"
                     /protein_id="HEZ7799547.1"
                     /translation="MIFKQDQNYWAVFYANEEALIVQTCSGLGLTAIDHLYPPHILPL
                     DTDNETLGTTVLQALANSRTLDNEAERIDFLKQENFKPRYEDWVAKLCGNLGYKTKRA
                     LFKNMMSVDIWLHNGCLKISPSRHVKLEAWDAIDADDVILSLDNSPEEIGAGLKLALS
                     RCR"
     gene            5092..6525
                     /locus_tag="WJL87_001121"
     CDS             5092..6525
                     /locus_tag="WJL87_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ7799548.1"
                     /translation="MNEYSQLIKHPDISLSPISDGIGVGNPATGEILAYVRKTDSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWCWYFLVKENKEALARLMTMEQGKSLTESRGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIIKPASLTPLSAYALASLAYEAGVPQDLLPVVSGRASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSGIYDAFCRKLSEKAAALKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALSKGAICLTGGKRSALGGTFFEPTVLSGITAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            6635..7423
                     /locus_tag="WJL87_001122"
     CDS             6635..7423
                     /locus_tag="WJL87_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ7799549.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVVEAFEQDRMTIVTAAEVVVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDQWLPYSDKEAEEIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            7791..9347
                     /locus_tag="WJL87_001123"
     CDS             7791..9347
                     /locus_tag="WJL87_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ7799550.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELAPQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            9364..10110
                     /gene="surE"
                     /locus_tag="WJL87_001124"
     CDS             9364..10110
                     /gene="surE"
                     /locus_tag="WJL87_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ7799551.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEKALWTLLAHFFKNPPQSPILWNIN
                     IPAVEPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGRVSDKEEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHAD"
BASE COUNT         2438 a         2519 c         2731 g         2431 t
ORIGIN      
        1 cttgtcctga tttttgttaa tccactatat tataaaaatt ccgtctgaag cggcgttcag
       61 acggaatttg tttcaagcag gcttaacagc agcgtccgcc gtttgcgccg cagcggcgtt
      121 tgcggcaata gtcttgaaac tgcagcttgg tcatcacagg ggcgttggga ttatgtttgc
      181 gctgctgtgc aacgtagttt tcataatcgg gcacgcctgc catcaagttt gctgtcagct
      241 tgatggtttt ccaccaagac gagagcttat gcttcacttt gtgcctccgg ctgtttgctg
      301 tcgcggtaca ccgccgggat ttctttggcg gtcggccagc cgactttgcg tgctttgagg
      361 gcggtacgca aaccgtacgc ggcgacaatc acgacaaccg acaagaagag gatggtcaga
      421 ccggcattaa tcttgtcgtt gaagatgatt tgcgccattt cgccgatgtc tttggcaggc
      481 gcaaggactt cgtttttagc caatgcgtcg ctgtatttgc cggcgtgggc aaggaagctg
      541 atgcgcgggt cgctgtggaa cagtttttgc aggccggcgt agcaggttac gaacagtacg
      601 ccgacggcgg gaacgagtac cacccaaaca taacggtcgc gtttcatctt aatcagcacc
      661 acggcgcaca taatcaaggc cacgcctgcc agcatttggt tggcgatgcc gaacaaaggc
      721 cagagcgagt tgatgccgcc cagcgggtcg gttacgccgg tgtagaggaa gtagccccac
      781 aatgccacgg cgaagaaggt cgcaatcagg ttggcgggga tggagtcggt gttgccgaaa
      841 ggtttgtaga agatgctgcc caagtcttga atcataaaac gtgcgacgcg cgtaccggca
      901 tcgacggcgg tcaggatgaa caaggcttca aacaataggg cgaagtgata ccagaacgcc
      961 atcatcgcct cgcccggaat caggcggctc ataatgtgcg ccataccgac tgcgagggtt
     1021 ggcgcaccgc ctgcacggga aaggatggtg ttttcgccca cttccttagc ggtatgcaac
     1081 agggttgccg catcgacagg gaattgcagc ttggtggtaa tcacttcggc ggcggtgttg
     1141 gcatccgtac cgatcagggc ggcagggctg ttcatggcga agtacacgcc gggatcaagc
     1201 gatgcggcgg cggcaagcgc cataatggct acgaaacttt ccatcaacat accgccgtaa
     1261 ccaatcatac ggacgtgggt ttcgttttcc agcattttcg gcgtggtgcc ggaggaaatc
     1321 agcgcgtgga agcccgaaac cgcaccgcag gcgatggtaa tgaacaagaa cgggaacaat
     1381 gcgcctgaga ataccggacc cgaaccgtcg ataaagtggg taaccgcagg catttgcaaa
     1441 gcgggattga cgatgacgat acccaaagcc aaggccgcaa tcgtaccgat tttcaggaag
     1501 gtagagagat agtcgcgcgg agtgagcagc aaccatacgg gcaatacgga ggcgacaaag
     1561 ccgtaaatca taatcgccca agtgagctgg atgccgtcaa ggtcgaacca atgtccgatg
     1621 gaacttttcg ccacatcttc gccgtaaatt accgccagca tcagcaaaat aaagccgacg
     1681 atggaaatct cgccgatttt gcccggacgg atatagcgcg tgtaaatacc cataaacagg
     1741 gcaatcggca tagtggcggc gatggtaaac gtaccccaag ggctgtgaac caatgctttc
     1801 acaacgatca atgccaacac cgccataatg atgaccataa tcatcaaaat accgatggat
     1861 gcaatcacgc cggggacagt gccgagttcc tgtttcacaa tatcgcccaa agacttaccg
     1921 tcacggcgca tagaaacaaa cagtaccatc atatcctgta ccgcgccggc aaacaccacg
     1981 ccgaagataa tccacaaagt accgggcagg taacccattt gcgccgccaa aaccgggccg
     2041 accaaagggc ccgcgcccgc aatcgcggca aagtggtgtc cgaacaatac gcctttgtgc
     2101 gtcggaacgt agtccaagcc gtcgttgtgg cgttctgccg gagtcaggcg gttaggatcg
     2161 agccgcatta cgcggttggc gatgtagagg ctgtaaaaac ggtaggcgat gcagtaaacg
     2221 gatatggcgg cggtaaccat ccataccgcg ctgacctgtt cgcctcggct gagggccaga
     2281 gtggtaaagg atgctaagcc gaccagtacc actatgcccc aaatgaggaa ggttttgagt
     2341 gatttcatcg aataaatcct tatctcacac tgtcggaata tgcctgaacg gcgggaaacg
     2401 gatgaccgcc tgccggcacg ggcacaaacc ggagtctgaa tgattgacga agtatgaaac
     2461 agtgcgcttg tcgggatttg tgccttatgc ccgcatcaaa cagtgcaggg atgcgggcgc
     2521 acaaaacgtt aaacgccgaa taggatttta acgcaaatta gcacactgat agcggttttt
     2581 acttggaatt tggaaaaaat ttacattcct tcgggcggac aggcgggttt ggacggcatc
     2641 gtcaggcaaa aggcggcgcc ggaagacggg taaagaaggg gcgcgcaatc cggattattg
     2701 attcatcgcg ggtaaattcc ggttatcggg cttgtgtgtt tgcgcgtccg tttatagtat
     2761 ggcgttgccg cagcttggaa tcagggcggt tgtttcatat cttattttta ttggggagct
     2821 tttatgaata tcaggttttt cgcactgacc gtgccggttt tgtctctggc ggcctgtgcc
     2881 gtgccggaga cgtatgatgg cggcggacgc gggcatatgc cgcccgttca aaaccaagcc
     2941 cgcccggcag attttcgggc attttcctgc gagaacggtt tgtctgtgcg cgtccgccgt
     3001 ttggacggcg gcagaatcgc gttgcggttg gacggcaggc gtgccgtcct ctcttccgac
     3061 gttgccgcat ccggcgaacg ctataccgcc gaacacggtt tgttcggaaa cggaaccgag
     3121 tggcaccaga aaggcggcga agcctttttc ggctttaccg atgcctacgg caattcggtc
     3181 gaaacttcct gccgcgcccg ttaaacgggg cagcctgttt gaaccgccgt ccggattttt
     3241 cgggcggttt tttatgtcgg atttgttttg cagttcggcc tccggcaggg ttttggctac
     3301 ctgataccgt ccgtaacttc gggtattgcc aagtttgtcg gcaacggccg ctgcgacttg
     3361 gtcggcgttt ctgccgaact cctgcgttac ttccctttgt aggttgatct ctttggcaac
     3421 cgcgtctttg tcgaagctgt ttttcagacg accttttgaa ttcatacaag ctatggcttg
     3481 cttatcttta taattgtcca aatttccaat aatcccgtcc ttcaattttc caataacaaa
     3541 attcgtccgg tattaaatac ccaaaatcca tcgcctttga aaaccagccg tcataaacat
     3601 aatcttcatg acatctgtcc caatccatat gtaagtatac ttcagtgtca aaattgacat
     3661 agaataaagg cagcctcgag gaaatctgat tgatttttaa gaaatcactt tgccggccgg
     3721 agtctgagat aggatcattg tacgacagct tgttctcgac aacggaatat aaccttaccg
     3781 cttcagcaga agtaaatccc tgaaacatca gttccttcaa ttcatccttt tttaatacaa
     3841 tgactgaatc cgattgaaga tagctttcaa aattgtcctt agttaactgc cagataccat
     3901 tacggtaatt tttataatca taattatatt gttcttttgt tataattcct tctcttagat
     3961 aaatatcaaa agatattttt gcatttaatt caaaattttc cttataatca atcaagtaat
     4021 atatcttttt cttatagaca aaaatagata tgcattgaat atactgaaag atgtcatcca
     4081 ataattcaga tttaaatttc tcaatctttt ccatatttgc caaattcctt aataacttta
     4141 tcttcagcaa gccgtaaccc acattaaatc tgcaggtaag gtgtggtacg cacgccagtg
     4201 aacttgagtc taataaaaag gtcgtctgaa acatttttcc ggctttcaga cggccttttg
     4261 ccaaatatta tcggcagcgg ctcaatgcca actttaaacc tgctccgatt tcttcagggc
     4321 tgttatccaa tgataaaatt acatcgtctg catcaatggc atcccacgct tccagcttga
     4381 catggcggct cgggctgatt ttcaggcagc cgttgtgcag ccaaatatcc acgctcatca
     4441 tgtttttaaa tagggcgcgt ttggttttat agcccaagtt cccgcatagc ttggcaaccc
     4501 aatcctcata gcgtggttta aaattttcct gttttaaaaa atcaatacgt tcagcttcgt
     4561 tgtctaacgt cctgctgttt gccaacgctt gcaagaccgt tgtgcctaaa gtttcattgt
     4621 cggtatccaa tggcaggata tgggggggat ataggtggtc tattgccgtt aaccccaaac
     4681 ctgaacatgt ttgaacaatc agagcttctt cattcgcata aaaaactgcc caataatttt
     4741 gatcctgttt aaaaatcatt atttgatctc cgtaattttg actttaatgt ttcaattttt
     4801 gccatactct accacacgtt gcaactgcaa tctttgctct ttatatattg caccccatca
     4861 aagggctgca ttacttttct ttctaaagat tacttttgta taaaaagtat ttttcttaag
     4921 cgatagggat tacgtccgcg ccatattcag acatcgcccg gatgttgcgc tgcccgatcg
     4981 gatgcttcag acggcatcgg aagggtttgc ggttttggaa tatgaagatg ataatgtccg
     5041 cgagattgac ggcatttgaa agcggttatc ctacataccg gaggacacga aatgaacgaa
     5101 tattcccaat taatcaagca tcccgatatt tccctttccc cgatttcaga cggcatcggg
     5161 gtcggcaatc cggcgacggg tgaaattttg gcgtatgtcc gcaagacgga ttcggacaaa
     5221 ctgaaaaacc tgattcaaaa ggcggcggca gcgcaaaaat tatgggcggc gaaaactgct
     5281 ttggaacgcg ccgacatatt gtggtgttgg tattttttag ttaaagaaaa caaagaagca
     5341 ctggcgcgcc tgatgacgat ggagcagggc aaaagcctga ccgagtcgcg cggcgaaatt
     5401 gattatgcgg cttcgtttgt gcgctggttt gccgaagagg cgcggcggat tgacggcgat
     5461 gtgctgacaa gcgtgaaagc gtcgcaaaaa ctggtcgtgt tgaaacagcc cgtcggcgtt
     5521 accgctgcga ttacgccgtg gaactttccg tccgcgatga ttgcgcgcaa ggccgcgcct
     5581 gctttggcgg tgggttgcgc gatgatcatc aaacccgcat cgctcacgcc tttgagtgcg
     5641 tatgccttgg cttcgttggc ttacgaagcg ggcgtaccac aggatttgtt gcccgtcgtc
     5701 agcggccgcg catcggaaat cggccgtgaa tttgccacga acccgattat ccgcaaaatc
     5761 agcttcaccg gctctaccga agtcggtgcg aaaatttttg ccgacagcgc ggcggacatt
     5821 aaaaaactca gtttggagct gggcggcaac gcgccgttta tcgtgtttga cgatgccgat
     5881 ttggacaaag ccgtcgaagg tgcgctcgcc agcaagttcc gcaacagcgg tcagacctgc
     5941 gtctgcacca accgcgttta cgcccaatcg ggcatttacg acgcattttg ccgcaaattg
     6001 agtgaaaaag cagccgcgct caaattgggc aacggcttgg aggatggcgt aaaccaaggg
     6061 ccgttgattg aggaaaaagc ggtggaaaaa gtcgagcagc acatcgccga cgcgctctca
     6121 aaaggcgcga tctgtctgac cggcggcaaa cgcagcgcgt tgggcggaac gttttttgag
     6181 ccgactgtct taagcggcat tacggcacaa atggcggtgg cacgcgaaga aaccttcggg
     6241 cctttgtgtc cggtattccg ttttgaaacc gaagccgagg tcatcgaggc tgcgaacaat
     6301 acggaatacg gtttggcagc ttaccttttc accgccgaca ccgcccgcca atggcgcgtc
     6361 ggcgaagcct tggaatacgg catggttggc atcaatacgg gcttaatcag taatgaagcg
     6421 gcaccgtttg gcggcgtgaa acgcagcggt ttgggacgtg aaggcagcaa atacggtgcg
     6481 gacgaatatc tagaattgaa atatctgtgt atggatgtcg ggtgacggat gccgtctgaa
     6541 tggcagggtt tcagacggtg ccgcatttta agcagtctct atctgttgta caatgcgccc
     6601 tgtttttacg gttatttttg atgtgaatta agatatgatg gaaaacggaa aaacagtccc
     6661 gagatggcgt tttgccttga aaagtgcggg ttggcatctc ttaatcagcc tgtcggttgc
     6721 agggctggcg gcattgctgg tttttaaggt ttggtatcct tatccttatg ccgagctgac
     6781 gggagggctg tcgctttatc agctggtggt ggctgtcgat attgtatgtg gtccgctgct
     6841 gactttaatt ttggcaagcc cgaagaaaaa gacaaaggca cgcatggtcg atttttccat
     6901 ggtcggcatc atccagctgg cggctttggt gtacggtctg cacagcgttt cgctggcgcg
     6961 tcctgtggtg gaagcgtttg aacaggatcg tatgaccatt gttacggcgg cggaagtcgt
     7021 ggtcgaagat ttgcacaaag cccccgaagg gctgcaaagc ctgtcgtggt tcggcatccg
     7081 ccgcattgca ttgaaagaac ctgaggatgc ggatgagaag aacaagacgc tggatttgtc
     7141 cctgaaaggt atcgagccga gtatgcgtcc cgaccagtgg ctgccgtatt ccgacaagga
     7201 agcagaagaa atccgcaaac atctgaaacc gctgaaagtc ttggcggatg cgagaaaaac
     7261 gacggttgcg gacattctga aacaggcagg tctcgccgaa ggggaggagc tgtattacct
     7321 gccgtttacc agcagcaggc agaaagagtg gatagtcatt accgataaag agggcaacac
     7381 caaaggctac gcgccgatag acggcttcat catcacccct taagcgttgg gactccgtcc
     7441 gcactcgaac atccgttctt cgcggcggta gaatcagact gtatttgaga ggggcgcaac
     7501 ccctcaatca gggcaacaca taggggaagg ctttatgtgc cgtccgtgtg ttgaaacatt
     7561 tcaaaataag ctcatgccgt ccgaacatcc tttcagacgg tatggcattt tgccattgcc
     7621 ccgataagct gttaaactat ttaaattatt tcgaccgaag gtacacgcac atgcaagacc
     7681 ccgagcagag cagcaagccc gcccgccgtt ttttgtgcgt accgtctgtt caggcagctt
     7741 gagcgcttca ggctgccttt tcgcgtttga acttgaggaa tacgaaaatc atggatttca
     7801 gttggttggc agaaccgcat acctggatag gttttgccac gcttttggtg ttggaagttg
     7861 tattggggat agacaatctt gtctttgtgg cgattttggc aaacaaggtc cagcccgcac
     7921 ggcgcgaccg cgcacggatt atcgggctgg ggctggcagt cgtcatccgc atcattatgc
     7981 ttgcttttat ggcgcacatc atcacgctga ccgagccgct gttccaaatc ggcggcctcg
     8041 ccgtttccgg caaggacatg attatgctcg cgggcggtat tttcctgctt tacaaagcca
     8101 ccaccgaact gcatgaacgc ctcgaagggc acaaccgttt taccgttgcc gacaaccaaa
     8161 aaaaacacgc gccgttttgg ggcgtggtcg cgcaaatcct gatactggat gccgtgtttt
     8221 ccatcgattc ggtcattact gcggtggcga tggtcgatca tatcgtcgtg gcgatgggtg
     8281 cggtcgtcgt cgcgatggcg gtgatgattt ccgccagcaa actcttgacc gaatttgtag
     8341 acagacaccc taccgtcgtg atgctctgcc ttggtttttt gttgatgatc ggtttcagcc
     8401 tgattgccga agccttccat ttccacattc ccaaaggcta cctctacgcc gccatcggct
     8461 tctcgatttt aatcgaactg tttaaccaga tttcccagcg caacagccgc aaaaacgact
     8521 acatcggcag ctcgtggcgc aagcgcaccg ccgaaaacgt tttgggtatg atgggcatac
     8581 gcgaaagcgt gcttgccgat gcgggcggcg aatccgggga cgacgcgcat tttgaagaaa
     8641 acgaaaaatc gatgatacgc agcgtgctga cgcttgccga acgcccgatt atgggggtga
     8701 tgatcccacg ccgcgacatc gaacggctgg acatttccca aagccgcgaa gaacagtgtg
     8761 cccaactgca aaacacgcct tacagccgcc tgctcgttgt cggtaaggcg ggcgtggacg
     8821 aacctttggg ctacatcaac aaaaaagacc tgctgtccca actgctggaa acaggcggtc
     8881 tcgacattca gacggcattg cgccagccac tcgtcctgcc cgacagcacc accgcgctgg
     8941 gcgcaatcga actcttccgc caaagcagcg cggattatgc tttggtggtg gacgagttcg
     9001 gcgcggtatt gggcatggta accatgaaag acctgctcga aaccatcgca ggcgagttcc
     9061 ccgaagaatt tgagcgcgaa gaagaaccag ccgttcaggg gaatcccgat gaaagcctga
     9121 cggtggaagg cgcgttggaa tatgtggaac tcgcaccgca actcaacctg ccgcagcagg
     9181 aggaagatgc cgatttccat acggttgccg ggctgattat ggaagaattg caaaccatcc
     9241 ccgatgtcgg cgattttgcc gatttccacg gctggcggtt tgaagtggtc gaaaaagaag
     9301 ggcagcgcat cgagcgggtc aaaatcacca aattgcccga agaataagca ttcaggatag
     9361 aaaatgaacg ttttgatttc caacgacgac ggctacctct ccgaaggtat tgccgttttg
     9421 gcgcgcgtta cggcggaatt tgccaacgtc agggtggttg cgcccgaacg cgacaggagc
     9481 ggggtcagca attcgctgac gctggaacgc cctttgcagt tgaaacaggc gcaaaacggg
     9541 ttctactatg tcaacggcac gccgaccgac tgtatccaca tcgggcagtc tgtattttcg
     9601 gattttcagg ccgattttgt cttttcgggc atcaaccggg gcgcgaatat gggggacgac
     9661 acgctttatt cggggacggt tgcggcggca accgaagcct accttatggg catacccgct
     9721 gtggcgtttt ccttaaacga cgcttccgga cgctattggg caaccgcaga aaaggcactg
     9781 tggacattgt tggcgcattt tttcaaaaac cccccgcagt cccctatttt gtggaacatc
     9841 aatatccccg ccgtcgagcc ggaagatgtg cggggcatta aaatcgcccg tttgggcagg
     9901 aggcatcacg gtcagaacgt catccccgcg cgcaatccgc gcggcgaaca gatttattgg
     9961 ataggcccgg taggcagggt ttccgacaaa gaagagggaa cggatttcgg tgaatgcggc
    10021 gcaggtttca ttaccgtaac gccgctgcaa atcgacctga ccgcctatcc ggacatggcg
    10081 gaaacagcgg cgttctggca tgcggactga ccgtttcat
//
LOCUS       DAWKST010000065         9985 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.065, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000065 DAWKST010000000
VERSION     DAWKST010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9985
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.065"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            50..289
                     /locus_tag="WJL87_001125"
     CDS             50..289
                     /locus_tag="WJL87_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799552.1"
                     /translation="MNVWDIWGRMVEIYHKYKKPCLVLAVDFVMGMVFIEPNEEPCIG
                     RCYAPMSESPDFANAVAMAVAMICIVWIAVLSMTD"
     gene            503..1804
                     /gene="bioA"
                     /locus_tag="WJL87_001126"
     CDS             503..1804
                     /gene="bioA"
                     /locus_tag="WJL87_001126"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ7799553.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLVDGMSSWWCAIHGYNHPVLNQAVETQIKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYRQVRGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPTSRFDDEWDEADLQPVRALFEVHHADIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELDKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
     gene            1801..2448
                     /gene="bioD"
                     /locus_tag="WJL87_001127"
     CDS             1801..2448
                     /gene="bioD"
                     /locus_tag="WJL87_001127"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ7799554.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGMLAKQLLQQGKSVITQKPVQTGC
                     QDIAEDIAVHRKIMGIPMQEADKRRLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEIVLVEGAGGLMVPLTEKLLTIDYIQQQAYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLHSLVFNHIHDSRDAHIAQYSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            2463..2939
                     /locus_tag="WJL87_001128"
     CDS             2463..2939
                     /locus_tag="WJL87_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ7799555.1"
                     /translation="MEHLFGKWLPDLPAAVSDGIDLPMSRLLKARSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDRIPVVEARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            2969..3859
                     /gene="ubiA"
                     /locus_tag="WJL87_001129"
     CDS             2969..3859
                     /gene="ubiA"
                     /locus_tag="WJL87_001129"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ7799556.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVGNSVPVEAWILFTANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            4044..4493
                     /gene="ptsN"
                     /locus_tag="WJL87_001130"
     CDS             4044..4493
                     /gene="ptsN"
                     /locus_tag="WJL87_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ7799557.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            4497..5459
                     /locus_tag="WJL87_001131"
     CDS             4497..5459
                     /locus_tag="WJL87_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ7799558.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGNLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            5440..6294
                     /gene="rapZ"
                     /locus_tag="WJL87_001132"
     CDS             5440..6294
                     /gene="rapZ"
                     /locus_tag="WJL87_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ7799559.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEEAVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYVVEKLARRLKGRYELLIRHR
                     QAQNLSDR"
     gene            6375..7316
                     /gene="ylqF"
                     /locus_tag="WJL87_001133"
     CDS             6375..7316
                     /gene="ylqF"
                     /locus_tag="WJL87_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ7799560.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTRAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEEGGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            7417..8019
                     /locus_tag="WJL87_001134"
     CDS             7417..8019
                     /locus_tag="WJL87_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ7799561.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLMEMPPTDEEEPEAVPSDTLPN"
     gene            8204..9877
                     /gene="recN"
                     /locus_tag="WJL87_001135"
     CDS             8204..9877
                     /gene="recN"
                     /locus_tag="WJL87_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ7799562.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKSVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSREQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIALLPSSPTSHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRVEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
BASE COUNT         2407 a         2532 c         2737 g         2309 t
ORIGIN      
        1 atagggctgt aaccttcaat cggaatttgt tgcctgcggg atatgcggta tgaatgtttg
       61 ggatatatgg ggcaggatgg tggaaatcta tcataagtat aagaagccgt gcctggtttt
      121 ggcggtggat tttgtgatgg gtatggtatt catagagccg aatgaggagc cgtgcatcgg
      181 cagatgctat gcgcctatgt cggagtcccc tgattttgcc aacgctgttg cgatggctgt
      241 tgctatgatc tgtatcgtat ggattgccgt tttgtctatg actgattgaa gcgttgggcg
      301 gaggctgcgt gaaacggtat tgggcgttgg gccgtctgat tccaatcggg cttgaggaat
      361 gcgaaacggt gtgcgcttat gttgcggaac gatttgtttc gcggttttgc gcccgaaacg
      421 gatggagagg tgtgggaaac ggtctgtcgg agtagaatac gcgtttgcgt ttgaatgcag
      481 caataagaaa agagagaaac ttatgccgtc tgaacatcaa cacacatcat cattacttga
      541 tttcgaccgt acccatctgc tccatcccta tacttccatg accgatccgc ttcccgttta
      601 tcctgtcaaa cgtgcagaag gggtgtttat cgaattggcg gacggcacgc ggctggttga
      661 cgggatgtcg tcctggtggt gtgcgataca cggttacaat catcctgttt tgaatcaggc
      721 ggttgagacg cagattaaac aaatgtcgca tgtgatgttc ggcggtttga cgcatgagcc
      781 tgcggtggag ctgggtaaat tgttggttgg gattttgccg caggggttgg accgtatttt
      841 ttatgcggat tcaggttcgg tttcggtgga agttgcgttg aaaatggcgg tgcaataccg
      901 gcaggtgcgg gggttgacgg cgaagcagaa tattgcgacg gtgcggcgcg ggtatcacgg
      961 cgatacttgg aacgcgatgt ccgtctgcga tccggaaacg gggatgcacc atattttcgg
     1021 cagcgcgttg ccgcagcgtt attttgtcga taacccgaca agccgtttcg acgatgaatg
     1081 ggacgaggcg gatttgcagc ctgtccgcgc cttgtttgaa gtgcatcatg cggatattgc
     1141 cgcctttatt ttagagccgg tcgtgcaggg cgcgggcggc atgtattttt atcatccgca
     1201 gtatcttcgc ggattgcgcg atttgtgcga cgagtttgat atcgttttga tttttgatga
     1261 aatcgccacc ggattcgggc gcacgggcaa gatgtttgcc tgcgaacacg cggaggtcgt
     1321 gccggatatt atgtgcatcg gcaagggttt gagcggcggc tatatgacgc tggcggcagc
     1381 aatcacttcg caaaaagtta ccgaaacgat ttcgcgcggc gaagcgggcg tgtttatgca
     1441 cggcccgacg tttatggcaa acccgctggc gtgtgccgtt gcctgcgctt cggtcaaact
     1501 gcttttgtct caagactggc aggcaaatat ccgccgcatt gaaagcatct taaaaggccg
     1561 tctgaaagcc gcgtgggaca ttcgcggcgt gaaagacgta cgcgttttgg gtgccatcgg
     1621 cgtgatcgag ctggataaag gcgtggatat ggcgcgtttt caagcagatt gcgtggcgca
     1681 gggcatttgg gtgcgcccgt tcggcaggct ggtatatctg atgccgccgt atattatttc
     1741 agacggcgtt ttgaccaaac ttgccgacaa aaccgtgcaa atcttgaagg aacacagcaa
     1801 atgaaaggcg tttacttcgt cagcggcata gacacggaca tcggcaaaac tgtcgccacc
     1861 ggtatgttgg caaaacaatt gttgcagcag ggcaaaagcg tgattacgca aaagcccgtg
     1921 caaaccggtt gccaagacat cgccgaagac atcgccgttc accgcaaaat catgggcata
     1981 cccatgcagg aagccgacaa acggcggctg actatgcctg aaatcttcag ctatcccgcc
     2041 tcgccccatc tcgccgcccg tctggacggc agggctttgg acttggacaa aatccgtacc
     2101 gctacgcaag aattggcggc acaatacgaa atagtcttgg tcgaaggcgc gggcggtttg
     2161 atggttccgc tgacggaaaa gctgttaacc attgattata ttcagcaaca ggcttatccc
     2221 gtcatcctcg ttaccagcgg tcggctcggc agcattaatc acaccctgct cagtttcgcc
     2281 gcgctcaaac aatacggcat tcgcttgcac agcctcgtat tcaaccatat tcatgacagc
     2341 cgcgacgcac acatcgccca atacagcctg agctacttga aatgccgtct gaaagccgat
     2401 ttttccgaag cggaatggat ggagttggca aaaacagacg cggtataaag attgggaaaa
     2461 atatggaaca cctatttggg aaatggctgc ccgacttgcc cgccgccgtt tcagacggca
     2521 tcgacctgcc gatgagccgg ttattgaaag cccggtcgct gaccgccgca ttgcgcgcct
     2581 tgccgcatac attttcggtg gaactgctga aactgggcga attggagacg gaatgcggag
     2641 ggaggctggt gcgcgaagtt ttgttgaagc tggaccgtat ccctgttgtt gaggcaagga
     2701 gcgaatgccg gataggttcg gcgttttggc aaaacatttt ggactgcggc acgcgccctt
     2761 tgggcgagcg tctgtttcaa gccgatttgg aaggggcgcg ttcggcgttt gagtttgccg
     2821 tttccggcga aggatgcgga cggtactttg ccgcgcggcg ttctcggttt tcccgtcacg
     2881 gcgaggaaat gctgctgacc gagtattttc tgcccgaact gaaacgcttt atcggataaa
     2941 ataccgtttt ttcaagctgt gcggcaatat gaatcctaaa tcccctttat ttttacgact
     3001 gtccgatcgt ttggatgtgt acctgcgcct gatgcgggcg gacaagccca ttgggacgct
     3061 gcttttactg tggccgacct actgggcatt gtggctggct tcagacggca ttcccgattt
     3121 ggcggtattg gcggcgttta caatcggtac gtttttaatg cgcagtgccg gttgcgtcat
     3181 caacgatttt gccgaccgcg attttgacgg tgctgtcgag cgcaccaaaa accgtccgtt
     3241 cgcacagggc agggtcaaga aaaaagaagc cctgctgctg acggcatttt tgtgcctgct
     3301 tgccgcattg tgcctgattc cgctgaatca tctgacttgg ctgatgagcc tgcccgccct
     3361 gtttcttgcg ctgacttacc cgtttaccaa acgttttttt ccgattcccc aactctatct
     3421 cgggcttgcc ttttccttcg gtatcccgat ggcgtttgcc gcagtgggca acagcgtccc
     3481 cgttgaagca tggatacttt ttaccgccaa tgttttatgg acactggctt acgacactgt
     3541 ttacgccatg gcggacaaag aggacgattt gaaaatcggc atcaaaacct ccgccgtcac
     3601 gttcgggcgt tacgacatcg ccgccgttat gctgtgtcat ggcggcttta ccctgctgat
     3661 ggcagtattg ggggcggtta tcggtgcggc atgggcatat tggacggcaa tccccatcgt
     3721 cctgctgctg caataccgcc aatatgccgc catcaaaagc cgcgtccggc aaatctgttt
     3781 tgaaacgttt ttggcaaaca acagaattgg ttgggtgtgg tttaccgcca tttttgccca
     3841 tacgtttttc gcgaaataag gcagggcaat gccgtctgaa gagccgtaaa ctgctttgga
     3901 cggcatttct atctgtgccg aaaagcgtta aaatatgttt ttaaaacgct gtgttatgtc
     3961 agcccgtacc gtatgcggga ttgagatttg ccccggcagt cggtacaatc tttctgtttt
     4021 gcgatgtctg aaaagagaag cttatgagcc ttatcggcga aattttgcct ttgtcccata
     4081 ttgttttgga tatggaggtg ggcagtaaaa aaaggctgtt tgaggaagcg ggcctgcttt
     4141 tggaacgcga atcctcattg tcccatgctg atgttttcga atgtcttttt gcccgtgaaa
     4201 aactcggttc gaccggtttg gggcagggcg ttgccatccc gcacggccgt catgccggcg
     4261 tgaagcaggc gacgggcgcg ttcatccgca cgcgcgaacc cgtcggattt gacgcaccgg
     4321 acggcaaacc ggtttccctg gtttttatct tgctggttcc ggaaaatgcg acgggtgagc
     4381 atttggaggt attgtccaaa ctggccggca agttttccca aaaaagcatc cgcgaatcgc
     4441 tgatgacggt ttcttctgcg gaagaagccc gcgccatcct gaccgaagaa taaaatatgc
     4501 ccagtatctc tgtccgccga ctgtttgacg acaaccaata caaactgcaa ctcgcctggg
     4561 ccgccggcaa ttcgggtgcg gacaaccgta tcggcgtaga ggcggacaag cccgtcctcg
     4621 ctttggtcgg acacctgaat ttcatccacc ccaaccaaat ccaagtggtc ggtttggcgg
     4681 agtcggaata tctgaaccgc ctcgaatcgg gggaaacggg ttatcagttc ggaaacctgt
     4741 tcgacatttc tatgtctttg gtcattgtgg caaacggctt gccggtttcc ccgggactgc
     4801 gcgactactg ccacaaaaac gatattccac tgctgacttc caaactcgaa agcccctatc
     4861 tgatggacgt gttgcggatt tacctgcaac gcaccctggc ggcatcgtcc gtcaaacacg
     4921 gcgtatttct cgatgtgttt gaaatcggcg tgctgattac cggacattcg ggtttgggta
     4981 agagcgaatt ggcgttggag ctgatttcgc gcggccacag cctgattgcc gacgatgcgg
     5041 tcgagctgtt ccgcatcggc ccggaaacgc tggaagggcg gtgttcgcct atgctgcgcg
     5101 attttttgga agtgcgcggc ttggggatac tcaatatccg ccatattttc ggcgaaactt
     5161 ccatccgccc caaaaaaatc ctgcaactca ttatcaattt ggtcgaggcg gacgacgagt
     5221 atatgaagca gctcgaccgt ttgagtatcc gtaccgaaac cgaatccatc ctcaacgtca
     5281 acgtccgttc ggtaacgctg cctgtcgcgg tcggacgcaa ccttgccgta ttggttgaag
     5341 cggcggtgcg caattatatt cttcagttgc gcggtaagga tagtacgcgc gaatttttgg
     5401 aacgccatca gacgcagctt aaagaaaacg aacagaatca tgaaaatcgt cctgattagc
     5461 gggttgtccg gttcgggcaa atccgtcgca ctgcgccaaa tggaagattc gggttatttc
     5521 tgcgtggaca acctgccctt ggaaatgttg cccgcgctgg tgtcgtatca tatcgagcgt
     5581 gcggacgaaa ccgaattggc ggtcagcgtc gatgtgcgtt ccggcataga catcggacag
     5641 gcgcgggaac agattgcctc tctgcgcgga ctggggcaca gggttgaagt tttgtttgtc
     5701 gaggcggaag aggccgtgct ggtccgccgg ttttccgaaa ccaggcgagg ccaccctctg
     5761 agtaatcagg atatgacctt gttggaaagc ttaaagaaag aacgggaatg gctgttcccg
     5821 cttaaagaaa tcgcctattg tatcgacact tccaagatga atgcccaaca gctccgccat
     5881 gcagtccggc agtggctgaa ggtcgaacgt accgggctgc tggtgatttt ggagtccttc
     5941 gggttcaaat acggtgtgcc aaacaacgcg gattttatgt tcgatatgcg cagtctgccc
     6001 aacccgtatt acgatcccga gttgaggcct tacaccggta tggacaagcc cgtttgggat
     6061 tatttggacg gacagccgct tgtgcaggaa atggttgacg acatcgaaag gtttgttacg
     6121 cattggctgc cgcgtttgga ggatgaaagc aggagctacg ttaccgtcgc catcggctgt
     6181 accggcgggc agcaccgttc cgtctatgtt gtcgaaaaac tcgcccgaag gttgaaaggg
     6241 cgttatgaat tgctgatacg gcacagacag gcgcaaaacc tgtcagaccg ctaattccgt
     6301 caaaccatta tgccgtctga aaccccgtcc tccgtttcag acggcatttc aaatattgaa
     6361 aagaaagaac agacatggca atccaatggt ttcccggcca tatgaacaag gcgaaaaaag
     6421 ccatcgccga gcgtgcaaaa agcgttgata tggtgattga aatgctggac gcgcgtatgc
     6481 ccgcctccag cgaaaacccc ctgcttgccc agctttccaa aggcaaaccc aaacttaaaa
     6541 tcctaaacaa acaagatctt gccgaccccg aacgcacaaa ggtctggctt gaccactaca
     6601 acagccgccc cgacacgcgc gccattgccc tcgattcctc cgaaaccggc gcacacggca
     6661 aaatcaccca agcctgccgc gcgatgattc cccaccggca aggcatagac aaacccttgc
     6721 gcgtcttgat ttgcggcatt cccaacgtcg gcaaatccac cctcatcaac ggcatgatag
     6781 gcaaaaaatc cgccaaaacc ggcaacgaac ccggcatcac caaagccgaa caacgccttt
     6841 tccttgccga cgacttctgg ctctacgaca cccccggaat gctgtggccg aaaatcatcg
     6901 tcgaagaagg cggctacaac cttgccgccg gcggcgcagt cggacgcaac gcgttggacg
     6961 aagaagaagt cgccctcgaa cttttagact acctccgccg ccactacctc cctatgttgc
     7021 aagaacgcta ccaagccgac aaagacccca gcagccactg ggacgaaaac gtttggctcg
     7081 aatggatagc caaaaaacgc ggcgcagtcc tcagcggcgg acggatcaac taccaaaaag
     7141 ccgccgaaaa catcctcacc gacttccgtg aaggcaaaat cggcagaatc accctcgaaa
     7201 cgccgaacca atgggaaact tggctcaaaa aagcccgtca gaaagaagcc gaactcaaag
     7261 ccatacgcga agccagaaaa gcagagcgta aagggcagaa gccttcggaa gcataaagaa
     7321 tgccgtctga aaaatatttt tcaggcagct tctctctact ccaaccgatt tcagacggca
     7381 tatccaaacc catgccgttt cagcacggat acccgtatga ccgacaaaat ttctcccgac
     7441 gcactaattg aagccgcact gctgacccaa accgaaccgc tgaccgaaaa atctatgcgc
     7501 gaattgtgtg tgccgccact gtcgcaggac aaactgattg atgtgttggc gcagttgaaa
     7561 acgcgttggc aggatagggc gttgcaactg gtgcatacgc aagagggctg gcgttttcag
     7621 attgttcaga cggcattcga gcggctgggc agcctgcaag aacagcgtgc gccgcgttac
     7681 tcccgcgccg tgatggaaac actggcgatt atcgcctacc agcagcccgt aacgcgcggc
     7741 gacatcgagg gcatacgcgg cgtggcggtg tcgcagaacg tgatgcagac cttgcaggat
     7801 cgggggtgga ttgaagtcat cggacatcgg gacacattgg gaaaacccgc attgtgggcg
     7861 acaacggcaa cttttctcag cgatttgcgc ttggacggtc tggaagaact tccgccgctg
     7921 accgaactgg gcgaactggt tttacccgat ttaatggaaa tgccgcctac ggatgaagaa
     7981 gagccggaag ccgtaccgtc cgataccctg cccaactgaa attccaaatg ccgtctgaaa
     8041 cgcacattgc ttcagacggc attgcaacaa ataagcagat aaaaacaagt actaagcaaa
     8101 attaaggaaa acttattttt aatttaaaaa aaatcttagt tataattcgt atatctaaag
     8161 ttgatattgc ttttgtcggt agaattgcta aggaatcctc acaatgcttc taacactttc
     8221 tttgcgtgat tttgtcattg ttgaaaatct gaatctggat tttcaaagcg gctttaccgt
     8281 attgaccgga gaaaccggcg cgggcaagtc cattactttg gatgcgattg gtctgctgtt
     8341 gggcgacaaa gccgattaca gccaagtccg cagcggtgca aaagaagcgc agttgtcggc
     8401 attgttcgat atttcccatt tgcctgtttt aaaagcagaa ttgtatgaac aggggctttt
     8461 aaacgacgga gaagaagaac tcagtatccg ccgcattatc gatgccaaag gcaaaagccg
     8521 cagctttatc aacaatcagg ccgctacctt ggcgcaactc aaatccgtcg gtagccagct
     8581 tatcgacatc cacgggcaaa acgcccatca ttcgcttaat caggaagccg cccagcgcga
     8641 attgttggac gcatttgcgg gtagcaggga gcaggcggaa accgtcaggc agctttatca
     8701 aaattgggcc aatgcgaaaa aagccctcca agaggcgcag gaacacgccg atgccgtcat
     8761 tatcgagcgg gagcgtctgg aatggcagtt taacgaactg aatcagttgg acattaaaca
     8821 aggcgaatgg gaagccctca gccaaagcca cgacagcctt gcccattctg ccgagctgtt
     8881 gcaggctgcc gaagaagtcg gaagcaagat tgacggcgac aacggcatcc aacgccatat
     8941 ctatcaatgt caaaaactat tggccaatct gcaaaacatc gagccgcgct ttgccgagag
     9001 cctgaatatg ttggcaagca tcgaagccga attgggcgaa atcagtgcca atatgcgcga
     9061 tgtggcaggt cgcagcgaca tcaatcccaa tgaattggcg gcacaggaac agcgcatggg
     9121 cgagctgatg gggatggcgc ggaaataccg gatcgagcct gaagagttgc ctgccaagtt
     9181 ggcagaaatc gaagaacgcc tgcaaagcct gcaagctgcc gccgatttgg acgcgctcga
     9241 gcataatgtt gcccacaatt ttgccgaata tcaggaagct gcccacatcc tttctgccat
     9301 gcgccatcag gcggcagagc gtttgagcgg cgaaacgacc gagcatatgc aacaccttgc
     9361 catgaaaggt gcgcgttttg acattgccct actgccgtct tccccgacgt cgcacggctt
     9421 ggagcaagta cagtttcaag ttgccgccaa caaaggcaat ccgccccgtc cgctgaataa
     9481 agttgcctcc ggcggcgaat tggcgcgtat cagccttgcc ttacaggttg ttgccagcca
     9541 atatacccaa gttcccaccc tgatttttga tgaggtcgat accggtattg gagggggagt
     9601 ggctgaaatg gtcggcaagg cattacgtgc gttgggcaga aaacatcagg tgcttgccgt
     9661 tacccatctt ccgcaagtcg catcctgcgg cgaaaaccac tggcgggtgc gcaagcacag
     9721 cgagggagag caaaccgtca gcgaaatcag tatattggat gaaatccaac gggtcgaaga
     9781 ggttgcccgt atgttgggcg gagaagtcat taccgatacg acgcggcaac atgcggcaga
     9841 attgctgcaa cttgcgtcga aaaatagttt attttaaaat caatcagtta aaaaataact
     9901 aaaaataaaa gtctaaaaca atagacagaa ctcagataaa tccgtattat cacgctttct
     9961 taatcacttg aacaagtgat tgtgc
//
LOCUS       DAWKST010000066         9982 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.066, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000066 DAWKST010000000
VERSION     DAWKST010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9982
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.066"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(129..464)
                     /locus_tag="WJL87_001136"
     CDS             complement(129..464)
                     /locus_tag="WJL87_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ7799563.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            593..1498
                     /gene="mtrA"
                     /locus_tag="WJL87_001137"
     CDS             593..1498
                     /gene="mtrA"
                     /locus_tag="WJL87_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HEZ7799564.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     VVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1560..2048)
                     /locus_tag="WJL87_001138"
     CDS             complement(1560..2048)
                     /locus_tag="WJL87_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ7799565.1"
                     /translation="MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2195..3097
                     /locus_tag="WJL87_001139"
     CDS             2195..3097
                     /locus_tag="WJL87_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ7799566.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPCWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3138..4268)
                     /locus_tag="WJL87_001140"
     CDS             complement(3138..4268)
                     /locus_tag="WJL87_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7799567.1"
                     /translation="MAKHLPLAVLTALLLAACGGSDKPPAEKPAPAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4473..7097
                     /gene="alaS"
                     /locus_tag="WJL87_001141"
     CDS             4473..7097
                     /gene="alaS"
                     /locus_tag="WJL87_001141"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ7799568.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAAFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGDAYPELKEKQVQIEEALKNEESRFAQTLETGMALLENALAKGSKKLDGEI
                     IFKLYDTYGFPYDLTADICRERNIELDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVDELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGRHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAVLANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKALTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPAVLDSVKDWVGAKLV"
     gene            complement(7186..8208)
                     /locus_tag="WJL87_001142"
     CDS             complement(7186..8208)
                     /locus_tag="WJL87_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ7799569.1"
                     /translation="MLTKKEFEKFLDEVCTVLRQEAKNAPFKSQDIFENRVRALCIAK
                     IPAYADIVINPEPVPQIFPDVPIGEYGVEVKFTLKDTWRSVANSIQEKNKADGVQHIY
                     VVFGKMGGIPDVKWQLYEDSVIHVRTSHVPRFEVEIESDRPSLFEGFGISYAEFADLD
                     MHEKMEYIRKYARNRLKEGERLWWIEDIDAVDSHDLPLEVRLYTSLPQEEKIRLRAEA
                     ALVSPRIVCSGRAKHKYDNAVLYLITYRGVLCHQARDLFSAGSVANSAEDYRGGNYVE
                     KSLKLIEHEIEKAALEMDDALIVEYWGESVPTEKRLEYWIKMLDDLADGWIPSESLFN
                     GRYKAK"
     gene            complement(8262..9413)
                     /locus_tag="WJL87_001143"
     CDS             complement(8262..9413)
                     /locus_tag="WJL87_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ7799570.1"
                     /translation="MTEFARKNWNKLAGDAIKAKMHEHGIGFIQLSELFLEQGESETA
                     NAICNKVNRGTFSHAFYLKCMELMTKQEKQEDREKKYKIISLFSGCGGMDLGFLGGFE
                     FLNRTYDKHDFDIIWANEIDEHAVRTYRHNLGNHIVHGDIWQHLDEIPTSADIIIGGF
                     PCQDISINGKGAGISGSRSGLYKAMLSAVERVRPKIFVAENVKGLLMKHHRKDLETVL
                     SDFRNLGYQVDYYLLNAADYGVPQTRERVIIVGRLPEIPEIQIEKTNTSNSYVTARQA
                     IGDLVDKVEDEIANHIWSRAEKSPDQGSRKLDAERAGYTIRAECHGNIQWHYELPRRI
                     SMREAARIQSFPDNFIFQAKLRATERQIGNAVPPVLGWHIAAAVSKALK"
BASE COUNT         2470 a         2634 c         2373 g         2505 t
ORIGIN      
        1 cttacccctt gtttctcaaa tgccgtctga aaaaacggct gcatatgttg tctacaaaat
       61 atcgggaagc ataacaggca aaatgccgtc tgaaatattt tcagacggca tttcttatcc
      121 gaaacggatt atttttgcgt ttcaaccgct tccaatgcac gcagggcata agtgtaagcg
      181 gcaccggcat tcagggcgat ggcggttgcc aatgcgcctg cgatttcgct gtcggtcgca
      241 ccggctttgg cggcagcggc ggcgtgaacg ctgatgcagc tctcgcaacg agtggtaatg
      301 gcaacggcga tggcaatcag ttcgcgcgtt ttggcatcaa gtgcttcggc ggcggcggcc
      361 tgttccaatg cgccgtaggc ctgcagcatt ttaggatgcg ccttacccag ctcgccgaac
      421 gattttttaa ccaatgcggt atgttctttc caatctttaa acattttctt ttcctttctc
      481 ttgcgtttaa ccctgatacg cgcttgcgta tctgttttcg atgtgcgtat tattgcaatt
      541 attcagttgt gtttctcgtt taatcatctc attttatggt tcaaaaagat ttatggacat
      601 tctggacaaa ctggtcgatt tcgcccaatt gacgggcagt gtggatgtgc agtgcctttt
      661 gggcggacaa tggtcggtac ggcatgaaac cttgcaacgc gaaggattgg tacacattgt
      721 tacatcgggc agcggctatc tctgcatcga cggcgaaact tccccgcgtc cggtcagtac
      781 aggggatatt gtatttttcc cgcgcggctt gggtcatgtg ttgagccacg acggaaaatg
      841 cggagaaagt ttacaaccgg atatgcggca gcacggtgcg tttacggtca agcagtgcgg
      901 caacggacag gatatgagcc tgttttgcgc ccgtttccgc tacgacaccc acgccgattt
      961 gatgaacggg ctgcctgaaa ccgtttttct gaacattgcc catccgagtt tacagtatgt
     1021 ggtttcaatg ctgcaactgg aaagcaaaaa acctttgacg gggacggttt ccgtggtcaa
     1081 cgcattgtcg tctgtcctgc tggtgcttat cctgcgcgcc tatctcgaac aggataagga
     1141 tgtcgaactc tcgggcgtat tgaaaggttg gcaggacaaa cgtttgggac atttaatcca
     1201 aaaggtgata gacaaaccgg aagacgaatg gaatgtcgac aaaatggtgg cggctgccaa
     1261 tatgtcgcgc gcgcaactga tgcgccgttt caaaagccgg gtcggactca gcccgcacgc
     1321 ctttgtgaac catatccgcc tgcaaaaagg cgcgttgctg ctgaaaaaaa acccggattc
     1381 ggttttgtcg gtcgcactgt cggtaggctt tcagtcggaa acgcacttcg gcaaggcgtt
     1441 caaacggcaa tatcacgttt cgccgggtca ataccggaaa gaaggcgggc aaaaataaat
     1501 cggggcttca aacgcaaatg ccgtctgaaa aggctttcat acagcatttg cgtaccgcgt
     1561 catttcaagg gctgcatctt catcacttcc atcaaaaagt tggtaaatgc ggggttgttg
     1621 ggtttgacat ccatattttt ccaacgctgc tgccagccgc gcaaggcatt ctggatatac
     1681 agcttggact gttccgtatt gattgcgccc cgctggctgt ctatcgccga acgcaggtag
     1741 atttcataca tactgtcatc gacggcattg cgtccgacca ggcgttttct gaagttgttc
     1801 agatattgcg ccgcctgaac cttggtcatt ttaccgatac ccacctgata gcccaagcgc
     1861 gtcgcttcat cgctgatttt ggcaacatcc gtccaatgcg aagaggcaag gcggaaacct
     1921 tttgcaggtg cttccgtttt gacggtattg ataggattca cggggatttc cgtcaatgtg
     1981 ggcacataaa tagactggca gccggaaaga actgccacaa tggaaagagg gataaggtat
     2041 tttttcatgc ccccattata atcaagtttg ccttgagaaa acaaattgtt cggcaagaaa
     2101 aataaaattt cggcatcaga agcaggcaaa aacacattcc acaagccttg ccgcaaggtt
     2161 tacaatccga ccgtccttat cgcaacgacc gtttatggat accgcaaaaa aagacatttt
     2221 aggatcgggc tggatgctgg tggcggcggc ctgctttacc attatgaacg tattgattaa
     2281 agaggcatcg gcaaaatttg ccctcggcag cggcgaattg gtcttttggc gcatgctgtt
     2341 ttcaaccgtt gcgctcgggg ctgccgccgt attgcgtcgg gacaccttcc gcacgcccca
     2401 ttggaaaaac cacttaaacc gcagtatggt cgggactggg gcgatgctgc tgctgtttta
     2461 cgcggtaacg catctgcctt tggccaccgg cgttaccctg agttacacct cgtcgatttt
     2521 tttggcggta ttttccttcc tgattttgaa agaacggatt tccgtttaca cgcaggcggt
     2581 gctgctcctt ggttttgccg gcgtggtatt gctgcttaat ccctcgttcc gcagcggtca
     2641 ggaaacggcg gcactcgccg ggctggcggg cggcgcgatg tccggctggg cgtatttgaa
     2701 agtgcgcgaa ctgtctttgg cgggcgaacc ctgctggcgc gtcgtgtttt acctttccgt
     2761 gacaggtgtg gcgatgtcgt cggtttgggc gacgctgacc ggctggcaca ccctgtcctt
     2821 tccatcggca gtttatctgt catgcatcgg cgtgtccgcg ctgattgccc aactgtcgat
     2881 gacgcgcgcc tacaaagtcg gcgacaaatt cacggttgcc tcgctttcct atatgaccgt
     2941 cgttttttcc gccctgtctg ccgcattttt tctgggcgaa gagcttttct ggcaggaaat
     3001 actcggtatg tgcatcatca tcctcagcgg cattttgagc agcatccgcc ccactgcctt
     3061 caaacagcgg ctgcaatccc tgttccgcca aagataaaaa atgccgtccg aacatccttc
     3121 agacggcata tcgggcttta tttccccgcc ttcacatcct gccactggcg caccataaac
     3181 ttcaatgccg ccggctggat aggcaccatg ataaagctgt ttttcaaatc ctcctcggtt
     3241 gggaaaatcg tattgtcgtt tttaaattcg tcttccatca gctcacgcgc cggcttgctc
     3301 gaaggcgcat aagtaacgaa attgccgttt ttcgccgaca cttccgggtc gaggaagtcg
     3361 ttgatgtatt tgtgcgcgtt ggcgacgttt ttcgcatctt tcggaatcac gaaagaatcc
     3421 acccaaatcc ccacgccctc tttgggcatc atcacgcgga ttttttcctt gccgcccgct
     3481 tcttcggcac ggcgtttggc gatgttcaaa tcgccgccga aaccgattgt tacgcaggta
     3541 tcgccgcgcg ccaaatcatc gataaagccg gacgaagtaa agcgtttgat attggggcgg
     3601 tttttcttga gcagggcggt tgcctccctg atgtcttccg tattgctgct gttcgggttt
     3661 ttacccaaat agttcaacac cataggatag atttccgccg cgctgtctaa ataactgata
     3721 ccgcattgtt tgagtttgga cgtgtattcg gggtcgaaca ccaaatccca ctggttgtcc
     3781 ggcagcttgt ccgtacccaa agcctttttc acgcgttcgg tattgatggc gaaggtattt
     3841 gtcccccaat aaaacggcac ggcgtattcg tggccgggat cgaccccgtc catcagcctc
     3901 atcatttcgg gattgaggtg tttataattg ggaatcagcg atttatcgat tttctgatac
     3961 gcacctgcct taatctgcct gcccacaaac gcattggacg gcgcgacaat gtcgtaaccg
     4021 gattttccgg tcagcacctt gctttccagc gtttcatcgc tgtcgtacac atcataagta
     4081 accttgatgc cgtttttctt ttcaaaatcg gcaacggttt ccggatcgac gtattccgac
     4141 cagttgtaaa ttttcaatac gtttcggttt tccgccggcg ccggtttttc ggcaggcggt
     4201 ttgtccgaac cgccgcacgc tgcaagcagc aaagcagtca ggacggcgag tggcagatgt
     4261 ttggccatta tcattccttg catatcgggt tggagaaagc ggtcattata gccgatattg
     4321 gcaacagggc ttcagacggc attcaaaatc ccgccacacc cttccgaaaa ccgccgcttc
     4381 catagctaga aacagggatt tgcggtaaga taccgccgtt cgttttccct gcttttacca
     4441 tgacaagaca tttgagagac attgaaaaaa ttatgaaaac ctccgaactg cgccaaaaat
     4501 tcctaaaatt ttttgaaacc aaaggccaca ccgtcgtccg ctcttccagc ctcgtgccgc
     4561 acgacgaccc gaccctgctg tttaccaacg cgggcatgaa ccagtttaaa gacgtattct
     4621 taggtttcga caaacgcccg tacagccgcg ccaccaccgc gcaaaaatgc gtacgcgcag
     4681 gcggcaagca caacgacttg gaaaacgtcg gctacaccgc ccgccaccac accttctttg
     4741 aaatgatggg caacttctcc ttcggcgact acttcaagcg cgacgcgatc cacttcgctt
     4801 gggaatttct gacttccccc gaatggctca atatccccaa agacaaactg ttggcgaccg
     4861 tttacgcgga agacgacgaa gcctacaaca tctggttgaa cgaaatcggt atgccgtccg
     4921 agcgcatcgt ccgcatcggc gacaacaaag gcgcgaaata cgcgtccgac aacttctggc
     4981 aaatgggcga caccggccct tgcggcccct gctccgaaat tttctacgac cacggcgaag
     5041 aaatttgggg cggcattccc ggcagccccg aagaagacgg cgaccgctgg atcgaaattt
     5101 ggaactgcgt attcatgcag ttcaaccgcg acgaacaagg caatatgaat ccgctgccca
     5161 agccgtccgt cgataccggc atgggcttgg aacgcatggc cgccgttatg cagcatgttc
     5221 acagcaacta cgaaatcgac ttgttccaag acctgctcaa agccgttgcc cgcgaaaccg
     5281 gcgcagcttt cagcatggaa gaaccaagcc taaaagtcat cgccgaccac atccgctcct
     5341 gctcattcct gattgcagac ggcgtattgc cttccaacga aggccgcggc tacgtattgc
     5401 gccgcattat ccgccgcgcc gtgcgccacg gttacaaact gggtcaaagc aaaccgttct
     5461 tccacaaact ggtggccgat ttggtcaaag agatgggcga tgcctaccct gaattgaaag
     5521 aaaaacaagt tcaaatcgaa gaagcgttga aaaacgaaga aagccgtttt gcccaaactc
     5581 tggaaaccgg tatggctttg ttggaaaacg cgcttgccaa aggcagcaaa aaactcgacg
     5641 gcgaaatcat cttcaaactc tacgatacct acggtttccc atacgacttg actgccgaca
     5701 tctgccgcga acgcaatatc gaactggacg aagcaggctt cgagcgcgaa atggaagccc
     5761 aacgcgcacg cgcacgcgcc gcacaaagct tcaaagccaa cgcccaactg ccttatgacg
     5821 gtcaagacac cgagtttaaa ggttatagcg aacgccaaac cgaatccaaa gtcctcgccc
     5881 tctacaaaga cggcgagcaa gtcgacgaat tgaacgaagg cgacagcggc gcagtcgtca
     5941 tcgactttac cccgttctat gcagaatccg gcggccaagt cggcgatgtc ggctatatct
     6001 tctcaggcga aaaccgcttt gaagtacgcg atacccaaaa aatcaaagcg gccgtattcg
     6061 gtcaattcgg cgtacaaact tcaggccgtc tgaaagtcgg cgacagcgtt accgccaaag
     6121 tggacgacga aatccgcaat gccaatatgc gcaaccacag cgcaacccac ttgatgcaca
     6181 aagccctgcg cgatgtattg ggcagacacg tcgaacaaaa aggctctttg gttaccgccg
     6241 aatccacccg tttcgacatt tcccatcccc aagcggtaac tgccgaagaa atcgccgaag
     6301 tcgaacgccg cgtcaacgaa gccgttttgg cgaacgttgc cgtcaacgcc gccattatga
     6361 gcatggaaga cgcgcaaaaa accggcgcca tgatgctctt cggcgaaaaa tatggcgacg
     6421 aagtacgcgt attgcaaatg ggcggtttct ctaccgaatt gtgcggcggc acgcacgttt
     6481 cacgcaccgg cgacatcggc ctcttcaaaa tcatcagcga aggcggtatt gccgcaggcg
     6541 tgcgccgtat cgaagccatc accggcctga acgcactcaa atgggcgcaa gagcaagagc
     6601 gtttggtgaa agacatcatt gctgaaacca aagcccaaac cgaaaaagac gtactggcga
     6661 aaatccaagc aggcgcggca cacgccaaag cattggaaaa agaattggca cgcgccaaag
     6721 ccgaactcgc cgtccacgca ggcgcaaaac tcttggacga cgcaaaagac ttgggcgcag
     6781 ccaaactcgt tgccgcccaa atcgaagccg acgcagccgc cctgcgcgaa atcgtaaccg
     6841 acctgaccgg aaaatccgac aacgccgtga ttcttttagc ggcagtaaac gacggcaaag
     6901 tctccctgtg cgccggcgta tccaaagccc tgaccggcaa agtcaaagca ggcgatctgg
     6961 ttaaatttgc agccgaacaa gtcggcggca aaggaggcgg cagaccagat ttggcacagg
     7021 cagggggcac ggatgccggc aaattgcccg ccgtgttgga tagcgtgaaa gactgggtcg
     7081 gcgcgaagct ggtttgatgt gggaaaggca gcctgaaagg gattcaggct gccttttgtg
     7141 ttaaagatgc cgtctgaaag atattttcag acggcctctg tcagtttatt tggctttata
     7201 cctaccattg aatagcgatt cagaaggtat ccagccatct gccaaatcat ccaacatctt
     7261 aatccaatac tctaggcgtt tttctgtcgg gacgctttct ccccaatatt cgacaattag
     7321 agcatcatcc atttccaaag cagctttttc aatttcatgc tcaatcaatt ttaaagattt
     7381 ttctacatag tttccgccac ggtagtcttc cgccgagttg gcaacgctgc ccgcactgaa
     7441 taagtcccgc gcctgatgac aaagcacacc gcgataggta atcagataca ggacggcatt
     7501 atcgtatttg tgcttcgccc tgcctgagca gacaatacgg ggggagacta atgctgcttc
     7561 agctcggagg cggatttttt cttcctgcgg cagggaagtg taaaggcgca cttccaaagg
     7621 cagatcatgg ctgtctactg cgtcaatgtc ttctatccac cataaccgct cgccttcttt
     7681 cagcctgtta cgggcatatt ttcggatata ttccattttt tcatgcatat ccaaatcggc
     7741 aaattcagca taggaaatac cgaaaccctc aaacagagac ggtcgatcag attctatttc
     7801 tacctcaaag cgcggaacat gcgatgtgcg gacatgaatt acgctatctt cgtaaagttg
     7861 ccatttcaca tcgggaatgc cccccatttt tccgaatacc acatagatat gctgtacacc
     7921 gtcagctttg tttttctctt ggatactgtt tgctacgctg cgccaagtat cttttagcgt
     7981 gaatttgact tctacaccat attcccctat tggaacatcg gggaaaattt gcggcacagg
     8041 ttcaggattg ataactatgt cagcataagc gggaattttt gcaatgcaca gcgcacgaac
     8101 tctgttttca aaaatatctt gggatttgaa aggtgcgttt tttgcctctt gcctcaatac
     8161 ggtgcaaacc tcgtctaaaa acttctcaaa ctcctttttt gtcagcataa tgcattacca
     8221 cttaaaaata taaagtagta attttatctt atttaacagg gttatttaag tgccttacta
     8281 acggcggcgg cgatgtgcca ccctaaaaca ggagggacgg cgttgccaat ttgccgttct
     8341 gtggcacgca attttgcctg gaaaataaaa ttgtcgggaa acgattggat gcgcgccgcc
     8401 tcacgcatgg agatgcggcg gggtaattcg taatgccact gtatgtttcc atggcattct
     8461 gccctaatag tgtagcccgc cctttcggca tccaattttc gactaccttg atcggggctt
     8521 ttttctgctc tactccaaat atggttggca atttcgtctt ctactttgtc gaccaaatcg
     8581 cctatagcct gtctagctgt tacatagctg ttgctggtat ttgttttttc aatctgtatt
     8641 tcgggaattt caggtagcct tccaacgatg ataactcgct cccgagtctg tggaacaccg
     8701 taatcggctg cattcagcaa gtagtaatct acctgataac ccaaattgcg aaaatctgaa
     8761 agcactgttt ccaaatcttt ccgatgatgc ttcatcagta gccctttgac attttcagca
     8821 acaaatattt tggggcgaac gcgttcaaca gcagacaaca tggctttata cagcccgctg
     8881 cggctgcctg aaatacctgc acctttacca ttaatagaaa tatcctgaca aggaaagcca
     8941 ccgataatga tgtctgcgct agtgggtatt tcatccaaat gctgccaaat atcaccatgt
     9001 acaatgtgat ttcctaagtt gtggcggtag gttctaacag cgtgttcgtc gatttcgttc
     9061 gcccaaataa tatcaaaatc atgtttgtca taagtacggt ttaaaaactc aaagcccccc
     9121 agaaaaccca aatccattcc gccacaaccg ctaaataaag aaataatttt atattttttt
     9181 tcgcggtctt cttgcttttc ctgtttggtc attaactcca tacatttaag ataaaaagca
     9241 tgggaaaaag tacctctatt tactttgtta caaattgcat ttgccgtttc actttcccct
     9301 tgctccaaga ataattctga taattgtata aagccaattc catgctcatg cattttggct
     9361 tttatggcat ctccagctaa cttgttccaa ttttttcgtg caaattcggt catggttaat
     9421 ctttcagtca ttcataaatg acattatttt agcgtttttt ataatttaag ccaagattta
     9481 aaatctacct ttaggtttac aggccgtctg aaaacaaaaa tctcgttttc agacgacctt
     9541 tttccgctaa aatcccccta ccgtccaacc cgaaacaagg agaaccatca tgcaagttcc
     9601 tacttccgcc atcatcaacg gcgaatttgc cgacaaatac ggcaaacgcg gcagcaagcc
     9661 gtagccagca cccctgtcaa ccgcgtgcgt ggctagacca cacaccctac cactgcgggc
     9721 gacgcaaacc ttaagattga ggtgtagggt gtgtgcggta cgcacgcacg cggtttttgt
     9781 tactaccgaa tgattccaaa ccatgccgta atccagtcag cagcaaacgc tgcttgggtc
     9841 atgtaccaac aaaactcaat atcaaaggcc gtctgaagaa taattttcag acggcttctc
     9901 tctatcaaaa gcagcctgca cattcaaaaa tcgaagtgca ggctgctttt tgtgatgatt
     9961 ggaaaaaaca cacactattt ga
//
LOCUS       DAWKST010000067         9875 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.067, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000067 DAWKST010000000
VERSION     DAWKST010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9875
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.067"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(312..545)
                     /locus_tag="WJL87_001144"
     CDS             complement(312..545)
                     /locus_tag="WJL87_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ7799571.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(711..911)
                     /locus_tag="WJL87_001145"
     CDS             complement(711..911)
                     /locus_tag="WJL87_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ7799572.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(1083..1958)
                     /gene="xerD"
                     /locus_tag="WJL87_001146"
     CDS             complement(1083..1958)
                     /gene="xerD"
                     /locus_tag="WJL87_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ7799573.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFSSVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYAGRAGIGYISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(2010..2450)
                     /locus_tag="WJL87_001147"
     CDS             complement(2010..2450)
                     /locus_tag="WJL87_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ7799574.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTLGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            2771..3709
                     /locus_tag="WJL87_001148"
     CDS             2771..3709
                     /locus_tag="WJL87_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ7799575.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            3838..4131
                     /gene="hfq"
                     /locus_tag="WJL87_001149"
     CDS             3838..4131
                     /gene="hfq"
                     /locus_tag="WJL87_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ7799576.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPTSTLVQVETVQQPAE"
     gene            4216..4782
                     /locus_tag="WJL87_001150"
     CDS             4216..4782
                     /locus_tag="WJL87_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7799577.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCRLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTHTETSIPALCATLSLLKEGGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLSYGFTNRKNNPPYLLAFEKLRQK"
     gene            4836..5210
                     /locus_tag="WJL87_001151"
     CDS             4836..5210
                     /locus_tag="WJL87_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ7799578.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRVFLLWKNPKKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            5220..5714
                     /gene="folK"
                     /locus_tag="WJL87_001152"
     CDS             5220..5714
                     /gene="folK"
                     /locus_tag="WJL87_001152"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ7799579.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDTRLTLPHPRAHERSFVIRPLAEILPDFVLGKHGKVAELSKRLGNQGIRL
                     LPDR"
     gene            complement(5839..6318)
                     /locus_tag="WJL87_001153"
     CDS             complement(5839..6318)
                     /locus_tag="WJL87_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ7799580.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDCKDNAYKRALSALSRKL
                     GRCKDKLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVFQI"
     gene            complement(6538..7275)
                     /gene="ubiE"
                     /locus_tag="WJL87_001154"
     CDS             complement(6538..7275)
                     /gene="ubiE"
                     /locus_tag="WJL87_001154"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ7799581.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDADSYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(7318..7686)
                     /locus_tag="WJL87_001155"
     CDS             complement(7318..7686)
                     /locus_tag="WJL87_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ7799582.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            7806..9425
                     /gene="mnmC"
                     /locus_tag="WJL87_001156"
     CDS             7806..9425
                     /gene="mnmC"
                     /locus_tag="WJL87_001156"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ7799583.1"
                     /translation="MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLREHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITQAEAEKIAGIPLSVPYDHPSCGLYWQHGVW
                     LNPPAFVRTLLNHPLIGLHEDTPLTDISHDGEKWIASTPNGIFSATHIIYCTGAHSPR
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALSESLFAANPNPQKHQGHAAIRCDSPDHLPLVGAL
                     GDIAAMQQTYAKLALDKNYRIDAPCPYLPNAYANTAHGTRGLATAPICAAAVAAQILG
                     LPHPFSQRLRHALHPNRTIIRAIVRRKDLTP"
     gene            complement(9422..9571)
                     /locus_tag="WJL87_001157"
     CDS             complement(9422..9571)
                     /locus_tag="WJL87_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799584.1"
                     /translation="MDSKGNLLGIPYSLFWEENVMGVFDILPSGLVCLFEYDFLWSGW
                     HAAKA"
BASE COUNT         2636 a         2995 c         1988 g         2256 t
ORIGIN      
        1 taccgattac acaaacacat atcatcttta cacaatcatg cttccatcaa cagtaaaaca
       61 tgatatgatt gccaacaata acatctcaca ataaattttc taatttttat tgaaaaaatc
      121 aataaataag aattctccca accgacaaat aaaataaaga aagggttaat atgcaacatc
      181 gaagaagact agcaatttac caagcatcca aacgtgcttc ctttaccggc aggtcatccg
      241 ccccgcaaaa acgtaaagaa cgttgatttg aaaaaaatgc cgcctgaagt cctgcttcag
      301 acggcatttt tttaccgttc gagaaactgt ttcaacctgt cctcatccaa aaaccagtgg
      361 cagatttcct catctcccgc caacaaaacg ggaaccagct cattgtattt ttcttccaaa
      421 acaggatttt catccacatc gaccacttcc agcccgaacc cgtattcatt ttgaaaaggt
      481 ttgagtgcat cgcgcatttt gtggcacaag ctgcaatatt cacgaaacat caaggtcaat
      541 ttcatccgcg tttccttatc tgtcaatttg cacacgccaa agccttagac gcagcagaat
      601 catggtctat ttgggaaaaa acaatgtttt caaggaagat gatactcaag tcctgccaaa
      661 aacagcaaaa atgccgtctg aacagttcag acggcatttc caaaaccgtt ttacgcttga
      721 acattgatac ccgccgtaac cgtcggttgc gcattgtgcg ccgtcagaaa cgaagcggca
      781 agctccccac agcagccgca gcagctcgct acgcccaatt gcgactgcaa atcgcccatt
      841 gtggtcgcgc cggcggcgat ggtttccttg atttgatggt cggtaacggc attgcagatg
      901 cagacaaaca ttttgtgctc cgtgtgttct caaactatcg taccgatagc ggcttttatt
      961 atcgtatgcg aatataaata aaaacggttc gcattgcaag gtcggtatac acggtttgtc
     1021 ttggtaattt tttatcaagt ttgcattttc gattgatttc ttattaaacc aaagtagaag
     1081 cctcagtttc gggaatggtg ttccttcact acaccctgca accatacgtt ggcaacatgg
     1141 gtataaatct gcgtcgtatt caaatcggca tgtcccaaca tatcctgaac cacgcgcaaa
     1201 tccaagccgt gccgcaccag atgcgtggca aaggcgtggc gcaggctgtg cgggctgatg
     1261 tacccgatac ctgccctgcc ggcatattct ttgacaatca tccatgccaa ctgacgggaa
     1321 atgcccgttt ttttctgact gacaaacaat gcgtcgcaat tcctgccttt cagcagaagt
     1381 gggcgtgcct ccgtataata gcgttccacc caatacgccg actcctgccc catcgggacc
     1441 atcctctgct tatcaccctt tcccagcgcg gtaatacagc ccctgtccaa atccacactg
     1501 ctgaagttca gcccgaccgc ctcgctgacg cgcaagccgg tcgcgtacat caattcgagc
     1561 agggccttgt cccgcaaacc gtgcggcgtg tcggtatccg gggcggcaag cagtcgggaa
     1621 atctgctgct cggtgatcag ggtcggaata ttcttgtcga ttttgggcgg tttcagcaaa
     1681 cgggtgggat tgtccgtcct tatgccttca cgctccatcc atatatacag gcgtttgcat
     1741 gccgataatg cgcgcgcctg cgaactccgt tgctctccgt caacataaac cgccgccgcc
     1801 aaatccgctt cgtccgcatc cttcagcatt ctgcccgatt gggacaggcg gcgggcgatt
     1861 ttttccaaat cgcgccggta accgtttaaa gtattctgac tgagccgcct gtccaaccac
     1921 agcgtttcaa gcagcctgtc gatcaaacct tcttccatac cgttccaaac aaatgccgtc
     1981 tgaatcttct tcagacggca tggttcacat tatcgggaaa gcgtttccaa tacttcctgc
     2041 gcgtgacccg ccactttgac tttccgccat tcatgggcga tttctccatc cttattcaag
     2101 acgaacgtac tgcgctcgat acctaacgac tctttcccgt acagtttctt caatttgatg
     2161 acatcaaaca ggcggcacac tgtttcatcc ttgtcgctca acagctcgaa ccggaaaccc
     2221 tgcttggcgc aaaaattctg atgcgccttt acgccgtcgc gggaaatacc gaccacggta
     2281 taacccaatg cctcaaactg ttccaaacgc gcattgaaat ccaagccttc cgtcgtacag
     2341 cccagcgtac tgtctttcgg ataaaaatac acgaccaaag gcagatgttc tgccgaatga
     2401 aaatccgcac cgctgctcga aggcagggta aattcatatt tcacatccat agtcctactc
     2461 ccgatattcc cattattcaa aacggcacgc agacgcaccg ccgcaattgc caaaccaacc
     2521 ccgattctac cgccccaaag gacaaggatt caaccgccgg aaacatccaa accgacacac
     2581 gacggcatga aaaatatcca tgtcaaacca caaaatatgt tccgatttaa aaacagaatg
     2641 ttataaaacc caatccccaa aacacaacaa gaccgcccgc ctacgggcag tctcctgtca
     2701 gacgacatac tttacagatg gctgtttttt caacaaaata acgccaatac tcaaaaatat
     2761 ggaatcaaaa atgtccatcc atactctgaa acgcctgccc tcatcgctgc tgctcggtct
     2821 ctgcctttcc ctgccgtcag cccacctttt tgccgacaac gacattttag ggcaattttt
     2881 agaacagaac atgcttacct cctccgatcc gatagaaata ttcgccgaaa gcacgataca
     2941 ccccaccaac acccaagcca ttacaggcgg tctgattctc tcctcacagt ctgccctggt
     3001 cgtcaacaac aaaaccggac agatactgta tcagaaaaac gccgacagga ttatgcccat
     3061 cgcctccatt tccaaactga tgagcgcgat ggtcgttttg gatgcaaact tggacatgaa
     3121 cgaaaccgtt accattacgc ccgacgaaat cgaccgcatc aaagggaccg gcagccgtct
     3181 tgccataggt acggcactta cacgcaaaga actgctgcac ctgagcctga tgagcagcga
     3241 aaaccgcgcc acccatgcat tgggcagaac ctaccccggc ggcatgggcg catttgtcgc
     3301 cgccatgaac cgcaaagccc aaagcctcgg tatgtacggc agccgctttt acgaaccgac
     3361 cggactcaac ttccaaaacg tttccaccgc caaagacctg agccttatgg tcaacgccgc
     3421 cgcccaatat ccgcaaatcc gcaccaactc gacttccaac tacgcctcgg tacagaccaa
     3481 aaacgggcag cagaactaca aaaactccaa tgcgctggtc agggaaggca tgtggaacat
     3541 cgaattgcag aaaaccggct acatacgcga agcaggcagg tctatggttg tcaaagccaa
     3601 cattcaaaat caacccgtta ccatcgtatt gctgaactcg cccacatccg ccacacgcgt
     3661 caacgacgcc cgcaaaatcg aatcgtggat gctgcagcaa cgctcctgac atacaaatgc
     3721 ccggcggaaa accgcccaaa cacaaaaaaa attggtaatt tttcctattc aaggctacaa
     3781 ttcgacacgc acagggcatc tgcccgattt cacaatcata atagcggagt taaaaatatg
     3841 acagctaaag gacaaatgtt gcaagatccc ttcctgaacg ccctgcgtaa agagcatgtt
     3901 ccggtttcga tttacttagt taacggtatc aaattgcaag gtcaggttga gtctttcgat
     3961 caatacgttg ttctcctgag aaacacttcc gtcacccaaa tggtttacaa acacgccatt
     4021 tccaccatcg taccggcacg ctccgtcaac ctacaacatg aaaacagacc ccaagccgca
     4081 ccgacttcga ccctcgtcca agtggaaacc gtccagcagc ctgccgaata atccgcacga
     4141 agcatgacgt gtcatatctt tcaatacctt accggacaac ggtaaggtat ttttattttc
     4201 agacagcatt taaaaatgtt attgcaaaac atccttccat tcgcccattg ccttttgcgg
     4261 aaggcacttc ccgaaggcgg caatgctttg gacggcaccg ccggcaacgg acacgacacc
     4321 cttttcctcg cacaaaccgc aggcatccgg gggaaagtgt gggcattcga catccagccg
     4381 caagccctga gccgcactcg atgccgtctg aaagaagcga gatacgagaa tgtgcaactc
     4441 atcttagacg gacacgaaaa cctgaaacaa tatgtttcag aaccactaaa tgccgccatt
     4501 ttcaactttg gctggctgcc cggcggagat aaaagcctta ccacccatac ggaaaccagc
     4561 attcccgccc tttgcgcaac cctctccctg ctgaaagaag gcggtatgct tattgccgtc
     4621 ctctatccgg gacacgaaaa cggcaaacag gaggcagaag caatcgaaca atgggcaaaa
     4681 aacctgcctc aagaacagtt tgccgttttg agttacggct ttaccaaccg gaaaaacaac
     4741 ccgccctatc tgttggcatt tgaaaagtta cgtcaaaaat aaatgtttgc ggtaaaataa
     4801 gccgattcta acccttattt tgatacgagc cgaccatgca gtatctgatt gtcaaataca
     4861 gccatcaaat cttcgttacc atcaccattt tggtattcaa catccgtgtt ttcctacttt
     4921 ggaaaaatcc aaaaaagccc ttggtcggct tttggaaggc actgccccac cttaacgaca
     4981 ccatgctgct gtttacggga ttgtggctga tgaaaattac ccatttctcc ccgttcaacg
     5041 cgccttggct cggtacaaaa atcctgcttc tgctcgccta tatcgcattg ggtatgatga
     5101 tgatgcgcgc ccgtccgcgt tcgaccaagt tctacaccgt ttacctgctc gccatgtgtt
     5161 gcgtcgcctg catcgtttac cttgccaaaa ccaaagtcct gcctttctga aacaccgtta
     5221 tgaacaacag acattttgcc gtcatcgccc tgggcagtaa tcttgaaaac cctgcccaac
     5281 aggtacgcgc cgcattggac acgctgtcgt cccatcctga catccgtctt aaacaggctt
     5341 cctcactgta tatgaccgcg cccgtcggtt acgacaatca gcccgatttt gtcaatgccg
     5401 tctgcaccgt ttccaccact ctggacggca ttgccctgct tgccgaactc aaccgtatcg
     5461 aggctgattt cggacgcgaa cgcagcttcc gcaacgcgcc gcgcacattg gatttggaca
     5521 ttatcgactt tgacggcatc tccagcgacg acacccgact caccctgccg catccgcgcg
     5581 cgcacgaacg cagtttcgtc atccgccctt tggcagaaat cctccctgat tttgttttag
     5641 gaaaacacgg aaaggttgcc gaattgtcaa aacggctggg caatcaaggt atccgtcttt
     5701 tacccgacag gtaattccgc acgcggatgc cgtctgaaag cctttcagac ggcatttttc
     5761 ctttgccgcc aacacgcgtg caaaaaaatc gccccttgga aaaggggcgc aaaaggaaca
     5821 caaaccacta ccaaaacttt aaatctgaaa cactgcctgc cgcatactgt atccgacagg
     5881 atataaagcc ctcactaaat cgtttcgaga aatccaaact tcttcatcgc cgacagaaaa
     5941 tctgcctttc tccggtacca gctccaacag aaacggttga accgccgtat gcaacttgtc
     6001 cttacaccgc cccagctttc tagacaaggc ggacagggcg cgtttgtagg cattatcctt
     6061 gcagtcaagc tcccgcgcac tgccgaccca gctcaaacga aggttgcggc tttcatcctc
     6121 aatcagcaaa atgcctgttt tgacttcccc cacctcgggc tcgcatgaaa gcgcaatata
     6181 atatttgccg tcgatgttga catacgccgc ttcatgcacc tgacagttgc agccggaagt
     6241 ggcaagcagc gaataaacag agtcccgatg cagtttcaaa atatcaaggg aaatctgttt
     6301 aaaaatactt tccggcattt ttccctcctt tcgtctgctg cgttgtttac tggattgact
     6361 gcattataca gacatctccc aatcttgcaa atgataattg ttcctatttt ttaaaaaatt
     6421 tataaattat tgatggatat gtgaattaat tttaagattt aaaatctctt tctcaaaaaa
     6481 acacaaaaat gccgtctgaa acgtgttcag acggcattgg cttcacaggc agtccgttta
     6541 aaatttcacg cccttatgca gcgcgacgat gcccgcactc atattgtgat aatccacgct
     6601 gtcgaagccc gcatccagca tcatctgttt caaagtttcc tgatcggggt gcatacggat
     6661 ggattcggcg agatactggt agctgtccgc atctttggca atcagtttgc ccatgaccgg
     6721 cagcagcttg aacgaataga aatcatacgc gccttcgagc ggcttgtaga ttttggaaaa
     6781 ctccaacacc agcaacgtgc cgccaggttt caaaacacga tacatctctt tcagcgcggc
     6841 atctttatgc gtcatgttcc gcaagccgaa cgccacggaa actaagttga aataattgtc
     6901 agggaaaggc agtttttccg catcggcaag cgacacgggc aaaatcatgc cttcgttcaa
     6961 cagacggtcg cgcccgacgg tcagcataga ggaattaata tcggtcagcc aaacctcgcc
     7021 ttccttgccg acccgtttcg cccaaccgcg cgacaaatcg cccgtaccgc ccgcaatgtc
     7081 caacacttta tcgccttttt tcaggtgcgc cgtgttgatg gtgaaatgct tccacaccct
     7141 gtgcagccct gccgacatga catcattcat aatgtcgtag tttttggcga cggagtggaa
     7201 cacttccgcc actttgccgg ctttctcatc ttcgttgacc gtgctgaacc cgaaatgcgt
     7261 tttttgtcca cccatattct aaccacctcc ctgataccgc cctacggcat cccaaactta
     7321 acaatctgat tgcttttcaa aacgcgacgc gcccgccgcc gccaatttgt ccaaatattc
     7381 ctgccacatc gcatcgtatc cgtatgccag tctgtgcaga tacgcccaat cgtaaagacc
     7441 gctgtcgtgc ccgtccgaaa aactgatttt cagcgcgtac tgtccgacag gctgcaaatc
     7501 cgtaatttgc acatctgcct taccggtctg caaaacatcc tgtcccacac cgtgtccgcg
     7561 cacttccgca ctcggcgaat agacgcgtaa aaattcggca ggcagatttt taggttcgtc
     7621 cccataagtc aaaaccaaca cggtacggtt tttcaacaaa cggatttcaa caggaatatt
     7681 attcaaaaaa cacctcggtt cggcatacaa tatccggcag gcgcaggcgt gccttcaaac
     7741 ggcgcgtatt ttaacagctt tatcccgata ccgatattca aacccacact ttaaacggca
     7801 ttgatatgga caatctcgta tgggacggcg ttcccgacat ccgcacactc gaccaaacca
     7861 tccgcaaaca cgcacacccg ctcaacctga ttgtctgcct ccccgataat cagattcccg
     7921 attttcaaac cgcacaagat gcttcggacg cggaatgccg tctgaagcac cgtttggatc
     7981 aggcaacgca gtgcctccag ttcgacagca tcaacctcat cgaacacatc ctgcccgatg
     8041 tccgcttctg gctggttccc ccttcacgca cccgccgcct gcgcgaacac ttccaccaca
     8101 tttcctggca gaccgaagcc atcccgcaaa ccgaaagtaa gcccgacaaa ccctggtttg
     8161 cacttccaca aacatccgaa cggcaaaaac cggaacacat cctcgttatc ggcgcgggca
     8221 tatccggcgc ggcaaccgcc cacgccttag catcgcacgg catttccgtt accgtattgg
     8281 aagcccgaaa agccgcccaa gccgccagcg gcaaccgcca agggctgctc tacgccaaaa
     8341 tctcgccgca cgacaccgaa cagaccgaac ttttgcttgc cggctacggc tacaccaaac
     8401 gcctgctcgg acacatcctg cccgaatccg aaacctgggg cggcaacggc atcatccacc
     8461 tcaattacag ccgcaccgaa caacaacgca atcacgaatt gggtttgcaa aaacaccata
     8521 accacctcta ccgcagcatc acgcaggcag aagccgaaaa aatcgccggt atcccactgt
     8581 ccgtcccata cgaccaccct tcatgcggac tctactggca acacggcgta tggctcaatc
     8641 cacccgcatt cgtccgcacc ctcctcaacc atccgctcat tggactacac gaagacacac
     8701 cgttaaccga catttcccac gacggggaaa agtggattgc aagcacgcca aacggcatat
     8761 tttccgccac acacatcatc tactgcaccg gcgcgcacag cccccgcctg cccgaaacca
     8821 acctcgccgc cctgcctctc aggcaaatac gcggacaaac cggcctcaca ccgtccaccc
     8881 cgttttccga acaactgcgt tgcgccgttt caggcgaaag ctacatcagc ccgtcgtggc
     8941 acggactgca ctgctacggc gcgagtttta ttcccaacag cagccatacc ggatggaacg
     9001 aagccgaaga agcctcaaac cgccaagcat tggcacacct taaccccgcc ctttccgaat
     9061 cattgtttgc cgccaaccca aacccccaaa aacaccaagg gcacgccgcc atacgctgcg
     9121 acagccccga ccaccttccc ctagtcggcg cactcggcga cattgccgct atgcaacaaa
     9181 cttacgccaa actcgcgctg gacaaaaact atcgcatcga tgccccatgc ccatatctgc
     9241 ctaatgccta cgccaacacc gcccacggca cacgcgggct tgccaccgcc cccatctgcg
     9301 ccgccgccgt tgcagcccaa atcctaggct tgccccatcc cttttcacaa cgcctgcgcc
     9361 acgccctaca ccccaaccgc accatcatcc gcgccatcgt cagaaggaag gatctaaccc
     9421 cttaagcctt tgccgcatgc catcccgacc acagaaaatc atattcaaat aaacatacca
     9481 atcctgacgg taaaatatcg aaaacaccca taacattttc ttcccaaaat aaggagtaag
     9541 gaatgcctaa caaattgcct ttgctgtcca agatgaatgt tccgtaacat tcgaagactt
     9601 ccttaaccct gccagccgtt tcggtttctg taccccctgt tagataagga aggaatgaaa
     9661 aagccctgtt gattgtatga agtaaaggga gttaattttt gttaatcttc ccttctcagg
     9721 aactcaatat ataaggatgt taatttttat ttcccttctc gaggactcaa tatagtggat
     9781 taacaaaaac cagtacggcg ttgcctcgcc ttgccgtact atttgtactg tctgcggctt
     9841 catcgccttg tcctgatttt tgttaatcca ctata
//
LOCUS       DAWKST010000068         9864 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.068, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000068 DAWKST010000000
VERSION     DAWKST010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9864
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.068"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(128..1174)
                     /gene="adhP"
                     /locus_tag="WJL87_001158"
     CDS             complement(128..1174)
                     /gene="adhP"
                     /locus_tag="WJL87_001158"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ7799585.1"
                     /translation="MKMQAVVVNKNVAGDVEIVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SSVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDEKLAFAKESGADLVINAA
                     KEDAAKVIQEKTGGAHVAIVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(1458..1847)
                     /locus_tag="WJL87_001159"
     CDS             complement(1458..1847)
                     /locus_tag="WJL87_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ7799586.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRIRLSEVK
                     STLLMNAQTMERYYRQKGTFENYDKNKLKQNKYFNVTLSKVSPDHFTLQADPNPTTND
                     GETCVVTLNDGGTIAAFGTNQSCPGFD"
     gene            2073..3251
                     /locus_tag="WJL87_001160"
     CDS             2073..3251
                     /locus_tag="WJL87_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ7799587.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKENATSKEDLESAQDAFAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSAPTIIQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            3317..5251
                     /locus_tag="WJL87_001161"
     CDS             3317..5251
                     /locus_tag="WJL87_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ7799588.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARAEKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHKAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLADLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAMSVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(5569..6351)
                     /locus_tag="WJL87_001162"
     CDS             complement(5569..6351)
                     /locus_tag="WJL87_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ7799589.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKINFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            6490..8679
                     /locus_tag="WJL87_001163"
     CDS             6490..8679
                     /locus_tag="WJL87_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ7799590.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSEPLPQGTRVLVPFCNKTVVGIV
                     WETDIAPDMDAARILGVQTTFSDEPPLPESWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQVNAQA
                     VKLIENWAEQGWIETTEAAKPVLRSYHGQASHSEFVLNANQQKASDAIQTALGRFRPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVGNRFADVPTAVLH
                     SQMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPLPDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQSGCPIILGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCSDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPVPFKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSQFGAAPMLMVRLA
                     ERERAQIFLESTSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            complement(8726..9799)
                     /locus_tag="WJL87_001164"
     CDS             complement(8726..9799)
                     /locus_tag="WJL87_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ7799591.1"
                     /translation="MRPFCVGATVLAILGALVFFISPGVVVLHRQIFLELMLPAAYGG
                     FLTAALLDWTGFSGNLKPATTLMAVLLLVAAVLLPFLPQLAAFFVAAYWLVLLLFCAW
                     LIWLARNTDNFALLMLLAAFTVFQTAYAATGDLNLLRAQVHLNMAAVMFVSIRVSVLL
                     GAEALKECRLKDPVFIPNIVYKNIAITFLLLHAAAELWLPAQTAGFTALAVGFILLAK
                     LRELHHHELLRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGGMMGGV
                     MMVWLTAGLWHSGFTKLDYPKLCRIAVPILFAAAVSRTVLMNVNPIFFITVPAILTVA
                     VFVLYLFTFVPIFQANAFTGDPE"
BASE COUNT         2336 a         2538 c         2838 g         2152 t
ORIGIN      
        1 gacgtgttgc gatacgggtt ttcagacggt atttcaaaca gaacagatta aaatcaaatc
       61 caaatccata aaaaatgccg tctgaacagc gttcagacgg catttcgatg tgtatcgcca
      121 cgtcaaatca gtggtgatga ccgcagccgc attctttttt catatcgatc accatacggc
      181 cggtgatttt gccttcgcgc atttcttgga aaatggcggg tgcttcatcc aaagcacgca
      241 gttggacttt cggcacaacc aaaccttccg cgccgaattg gaaggcttct tccaaatctt
      301 tgcgcgtgcc gaccaaagag ccgaccactt cgatgccgtc caaaaccaaa cgcgggatgg
      361 acaaatccat cgattccggc ggcagtccga tggcaaccac acgtccgccc gcgcggacgc
      421 aattcacggc agagttgaat gcggcggcgg atacggcggt aacaatggct acgtgtgcac
      481 cgccggtttt ttcctgaatc actttggcgg catcttcttt ggcagcgttg atgactaaat
      541 ctgcgccgct ttctttggca aaagccagtt tttcatcgtt gatgtcgatg gcgacgacgt
      601 gtgcgccaaa tacttttttc gcgtattgga cccccaagtt gcccaaaccg cccgcgccgt
      661 agatggcaat ccactgtccc ggacgaacac tggaaacttt aatggcttta taagtggtta
      721 caccggcaca agtaatgctc gaagcttgcg caggatccaa gccttctggg actttgaccg
      781 cgtaatcggc actcacgata cagtgggtcg ccatgccgcc gtcggcagta tagccagcgt
      841 tcaatacaga gcggcacagg gtttcgcggc cggtattgca gtattcgcaa gagccgcagc
      901 tttggaacag ccaggcgatg ctgacgcggt cgccgacttt cagatttttc acaccgtcgg
      961 caacttcttt aaccaaaccg atgccctcgt gtcccagcac gcggcccggt ttttcgccgt
     1021 agtcgcctgc cgcaacgtgc aggtcggtgt ggcacacgcc gcaatattcg acttcgacca
     1081 acgcctcgcc gtattccaac gggcgaacct cgcgttcgac tatttccaca tcgcccgcta
     1141 catttttatt cacaacaact gcctgcattt tcatgattgc gctccttagt tacggcaaaa
     1201 aaacctgtaa acggaatgtt gtccgatata agggtaagca tacgcttccg catctcacag
     1261 gtcaagtggt atgttgttga aaaatataga ttatatgtta tattataaca tcttggaaag
     1321 gcgcggcatc ggggcggttg ccggatgagg ggcggcaggt ttcaagtttg aaaaaccgga
     1381 tgtgcaaacc cataaagata ccgtctgaag ctgtgtccgg acggcatctt tacgggtttg
     1441 cgggcttcgg cggaggatta gtcgaagccg gggcaggatt ggtttgtacc gaaagcggca
     1501 atggtaccgc cgtcgttgag cgtaacgacg caggtttcgc cgtcgttggt ggtgggattg
     1561 gggtcggcct gaagggtaaa gtggtcgggg ctgactttgc ttaaggtaac gttgaaatat
     1621 ttgttttgtt tcagtttgtt tttatcgtag ttttcaaacg tccctttttg gcggtagtaa
     1681 cgttccatgg tctgcgcgtt catcagcagg gtggatttga cttccgacag gcggatgcgc
     1741 cggatgtagg ttttatagga agggtaggtg atgagcgtca ggatgccgag gatggcgacg
     1801 gcaatcatca gctcgagcag cgtaaagcct ttttgaacgt ttttcatagc aatgtgtttc
     1861 catttgtttg tcggtcggcg acattataac gccgtatcgg aaatggcgga atatgtaaac
     1921 ggattgaaat tttcgggaaa gcagattgta taagccattt aaaacaaatg gttattttta
     1981 ttgtcggcag tttgccgcct tggatggggc agggacttgc ggtagaatcc gcttccgatt
     2041 tatgggattg acgcatacag agaattgaaa acatggcaaa aatgatgaaa tgggcggctg
     2101 ttgcggcggt cgcggcggca gcggtttggg gcggatggtc ttatctgaag cccgagccgc
     2161 aggctgctta tattacggaa acggtcaggc gcggcgacat cagccggacg gtttctgcaa
     2221 caggggagat ttcgccgtcc aacctggtat cggtcggcgc gcaggcatcg gggcagatta
     2281 agaaacttta tgtcaaactc gggcaacagg ttaaaaaggg cgatttgatt gcggaaatca
     2341 attcgacctc gcagaccaat acgctcaata cggaaaaatc caaattggaa acgtatcagg
     2401 cgaagctggt gtcggcacag attgcattgg gcagcgcgga gaagaaatat aagcgtcagg
     2461 cggcgttatg gaaggaaaac gcgacttcca aagaggattt ggaaagcgcg caggatgcgt
     2521 ttgccgccgc caaagccaat gttgccgagc tgaaggcttt aatcagacag agcaaaattt
     2581 ccatcaatac cgccgagtcg gaattgggct acacgcgcat taccgcaacg atggacggca
     2641 cggtggtggc gattctcgtg gaagaggggc agactgtgaa cgcggcgcag tccgcaccga
     2701 cgattatcca gcttgccaat ttggatatga tgttgaacaa aatgcagatt gccgagggcg
     2761 atattaccaa ggtgaaggcg gggcaggata tttcgtttac gattttgtcc gaaccggata
     2821 cgccgattaa ggcgaagctc gacagcgtcg accccgggct gaccacgatg tcgtcgggcg
     2881 gctacaacag cagtacggat acggcttcca atgcggtcta ttattatgcc cgttcgtttg
     2941 tgcccaatcc ggacggcaaa ctcgccacgg ggatgacgac gcagaatacg gttgaaatcg
     3001 acggtgtgaa aaatgtgctg attattccgt cgctgaccgt gaaaaatcgc ggcggcaagg
     3061 cgtttgtgcg cgtgttgggt gcggacggca aggcggcgga acgcgaaatc cggaccggta
     3121 tgagagacag tatgaatacc gaagtaaaaa gcgggttgaa agagggggac aaagtggtca
     3181 tctccgaaat aaccgccgcc gagcagcagg aaagcggcga acgcgcccta ggcggcccgc
     3241 cgcgccgata aacgaatatg ccgtctgaac acggaaacgg tttcagacgg catttgttat
     3301 tgatttacgg aatattatga gcttgatcga atgtaaaaac atcaaccgct atttcggcag
     3361 cggcgagaac cgcgtccata ttttgaaaga catcagcctg tcgatagaga agggcgattt
     3421 tgtcgccatc atcgggcagt ccggttcggg caagtccacg ctgatgaaca tactcggctg
     3481 tttggatacc gccggttccg gttcgtaccg aatcgacggc atcgaaactg ccaaaatgca
     3541 gcctgacgag ctggcggcat tgcgccgcga acgcttcggt ttcatcttcc aacgctacaa
     3601 cctcttaagc tcgctgaccg caagggacaa cgtcgcgctg ccagccgtgt atatgggcgc
     3661 gggcggcaaa gagcgttccg cgcgtgcgga gaagttgttg caggatttgg gtttggcaag
     3721 caaagagggc aacaagcccg gcgaactctc gggcggacag cagcagcgcg tctccatcgc
     3781 ccgcgccctg atgaacggcg gagaaatcat cttcgccgac gagccgaccg gcgcgctcga
     3841 taccgccagc ggcaaaaacg tgatggaaat catccacaag ctgcacaaag ccgggcatac
     3901 cgtcattatg gtcacgcacg accccggcat cgccgccaat gccaaccgcg tcatcgaaat
     3961 ccgggacggc gaaatcattt ccgacacctc gaaaaatccc gaaatccccg caagcaatgt
     4021 cgggaggatt cgggaaaaag cctcgtggtc gttttattac gaccagtttg tcgaagcctt
     4081 cagaatgtcg gtgcaagcag tattggcgca caaaatgcgt tcgcttctga cgatgctcgg
     4141 catcatcatc ggtatcgctt cggttgtctc cgtcgtcgca ttgggcaatg gttcgcagaa
     4201 aaaaatcctt gaagacatca gttcgatagg gacgaacacc atcagcatct tcccggggcg
     4261 cggcttcggc gacaggcgca gcggcaggat taaaaccctg accatagacg acgcaaaaat
     4321 catcgccaaa caaagctacg ttgcttccgc cacgcccatg acttcgagcg gcggcacgct
     4381 gacctaccgc aataccgacc tgaccgcttc tttgtacggc gtgggcgaac aatatttcga
     4441 cgtgcgcgga ctgaagctgg aaacggggcg gctgtttgac gaaaacgatg tgaaagaaga
     4501 cgcgcaggtc gtcgtcatcg accaaaatgt caaagacaaa ctctttgcgg actcggatcc
     4561 gttgggtaaa accattttgt tcaggaaacg ccccttgacc gtcatcggcg tgatgaaaaa
     4621 agacgaaaac gctttcggca attccgacgt gctgatgctt tggtcgccct atacgacggt
     4681 gatgcaccaa atcacaggcg agagccacac caactccatc accgtcaaaa tcaaagacaa
     4741 tgccaatacc caggttgccg aaaaagggct ggccgatctg ctcaaagcgc ggcacggcac
     4801 ggaagatttc ttcatgaaca acagcgacag catcaggcag atagtcgaaa gcaccaccgg
     4861 tacgatgaag ctgctgattt cctccatcgc cctgatttca ttggtagtcg gcggcatcgg
     4921 cgtgatgaac atcatgctgg tgtccgttac cgagcgcacc aaagaaatcg gcatacggat
     4981 ggcaatcggc gcgcggcgcg gcaatatttt gcagcagttt ttgattgagg cggtgttaat
     5041 ctgcgtcatc ggcggtttgg tcggcgtggg tttgtccgcc gccgtcagcc tcgtgttcaa
     5101 tcattttgta accgacttcc cgatggacat ttccgccatg tccgtcatcg gcgcggtcgc
     5161 ctgttcgacc ggaatcggca tcgcgttcgg ctttatgcct gccaataaag cagccaaact
     5221 caatccgata gacgcattgg cacaggattg aggttggaca aagatgccgc ctgaagctgc
     5281 agaaccggtc attttggagc agaaacttat tggacaaaaa cggtttctta gattctacgt
     5341 tccagattcc cacttgcgtg ggaatgacgg cggtgtgttt cgtattgttc aatttattat
     5401 tttcaatcat tcaatgggtt aggatgtgtt tgttggcttg ctgtactggt ttaaatttaa
     5461 tccactataa atttaatcca ctataacttt cagtgcggat tggttttcag gcggcatttc
     5521 ccgcaaaaaa ggcttggaat tttccaagcc ttttgttttg cggatggatt attgattttt
     5581 gcggatgatt tcctccagtt ggggcatcgg gctgtagccg ctttggctgc gcccgttggg
     5641 gaagacgagg gtcggcgtgc cgttgaagcc gaattgttcg cccaaggaag tggtttccgc
     5701 gacgggattg tcgcagatgc tgccgccgac cgggaatttg cctttacgca tccaatccgt
     5761 ccacgctttg gcgcggtcgg gctgacacca taagatttgc gccttgcgcg cggcatcggg
     5821 gtgcaggccg gcaatgggca tcataaagct gtaaaccgtc acgtcggtca ttttttcaaa
     5881 ctcgtgttcc aagcgtttgc agaacggaca atcggggtcg gagaagacgg cgactttcag
     5941 cttgccgttg ccgcgcactt ctttgatggc tttgtccaaa ggcagggagg cgaagttgat
     6001 tttgttcaaa tcggcggcgc gttcttcggt caggtttttg cgcgtgtcga tgttgatgag
     6061 ttcgccgacg aacatatagc cgccttcggc atcggtgtag ataatctgcc tgccgctgac
     6121 gacgacttcg taaatgcctt tgaccggtgt ttcgctgacg ctcaacactt tcaaatcttg
     6181 ggcggaatag gttttttcca aacgcgcttt caaagaggcg gcaacggatt tgccggcgga
     6241 ctcggctttg acggcgggtt cggcgttggc attggaaacg ggcgtttgcc cgcaagccag
     6301 cagcgggagg acggtaaagg gggtcaagat tttgattaac ttggttttca tataaagatg
     6361 attgcgcgtg ttggaaaagc ggaattgtat caaatctctg ttgcgcctgc attgcgccta
     6421 ggctcaattt atcgtctgaa aatagcttat gcctgttaaa atacgcaaaa aaatgatttg
     6481 cctgcctgta tgatctacca ccgcatcgcc gtaaacgtgc cgctttcaga cggccttttg
     6541 acttattcac attccgagcc gctgccgcag ggaacgcggg tgcttgtgcc tttctgcaac
     6601 aagaccgtgg tcgggattgt gtgggaaacg gatattgcgc cggatatgga tgcggcgcgg
     6661 attttgggcg ttcagacgac cttttcggac gaaccgccgc tgcccgaaag ctggcgtgat
     6721 ttgttggcgt ttacgtcgcg ttattaccac tatccgacgg ggcaggcggt gtttgccgca
     6781 ctgccgcaag gtttgaagga aacgcgcgcg gtggaaatgc cgcagccgcc attgttttat
     6841 gctttaaacg aagcaggcag ggcgcaaacg ccgccgccgg cgcggttcaa caaaaaagcg
     6901 gctttgtggg acgcgctgct gtcgggcgaa atgacgatgg cagcgttgaa gcaggtaaac
     6961 gcgcaggcgg tgaaattaat cgaaaattgg gcggaacagg gttggattga aacgacggaa
     7021 gcggcgaaac ccgttttaag gtcgtaccac gggcaggctt cgcactctga atttgtgttg
     7081 aatgccaacc agcagaaagc ttccgatgcc attcagacgg cattgggacg cttccggccg
     7141 tttctgctgt acggcatcac cggcagcggc aagaccgaag tgtatttcga tgcgatggcg
     7201 aaagtgttgg ctcaggggcg gcaggtgttg tttctgttgc ccgaaatcaa cctcacgccg
     7261 cagcttttga agcgggtggg aaatcgtttt gccgacgtgc cgaccgccgt gttgcacagt
     7321 cagatggcgg caggcaggcg cacgcaggat tatttgcgcg cgatgttggg gcaggcgaaa
     7381 ttggtcatcg gcacgaggct ggcggtgttc acgcctttgc ctgatgtcgg gctgattgtg
     7441 gtcgatgagg aacacgacgg ctcgtttaaa caagacaacg aattgcgcta ccacgcccgc
     7501 gatttggcgg tgtggcgggc gaagcagagc ggctgcccga tcatattggg cagcgcgacc
     7561 ccgagtttgg aaagctggca caaggcgcaa agcggcgcgt accgcctgct gcaactgacc
     7621 gagcgcgccc ataccgctgc gcaactgccg caagtggaca tcctcaacgt aggccgtctg
     7681 aaactcgaca acggtttctc gccgcaagcc ttgcagcttt tgaaacagaa ttttgaagcg
     7741 ggcggtatgt cgttggtgta cctcaaccgt cgcggtttcg cgccagcgct gttttgcagc
     7801 gactgcggcc ataccttcgg ctgcccgaac tgctccgcca aaatggtgct gcaccaacgt
     7861 gcccgccaac tgcgctgcca ccactgcgac caccgcgaac ccgtcccgtt caaatgcccc
     7921 gactgcggca accaagatct gaccgccgtc ggacacggca cgcagcgcgt cgaagaaacc
     7981 ctgcgcgcct tcctgcccaa ggcagccgtc gtccgtgttg acagggacag caccgcgcac
     8041 aaaaacgact gggcggattt gtaccgccgc attgccgaca acgaaatcga cattctggtc
     8101 ggcacgcaga tgctcgccaa agggcatgat ttcgcgcggc tcaacctcgt tatcgtgttg
     8161 aacgccgacg gcagcctgta cagcgcggac tttcgcgccc cggaaaggct gttcgccgag
     8221 ctgatgcagg tgtccggcag ggcggggcgc gccgacaaac ccggcaaggt gttgatacag
     8281 acccaactgc ccgaacatcc cgtcttcgcc gccgtcaaag cgcaggatta tgccgtgttc
     8341 gccgaaaacg aattgaacga gcggcaaatg ttcgcgatgc cgcccttcgg tttccagacc
     8401 gccgtccgcg ccgatgcgcc gcgtgttgcc gatgcgatgg aatttctcaa cgccgccaaa
     8461 gaaaccctcg ccccgctgtt gcccgaaagc gtttcccagt ttggcgcggc tccgatgctg
     8521 atggtgcgcc ttgccgaacg cgaacgcgcg caaatcttcc tcgaatcaac atcccgacaa
     8581 gatttgcacc gagccgtgag tttgtgggtg caggtgttgc agcaaaacag ggacggcaaa
     8641 atccgatggt cggtggatgt cgatccgcag gaggcttgat tattggcaat ccgatgccgt
     8701 ctgaaaaccg tttcagacgg catttttatt ccggatcgcc cgtaaacgca tttgcctgaa
     8761 agatcggtac gaacgtaaag agataaagca caaacacggc gacggtcaga atcgcgggga
     8821 cggtgatgaa gaatatcggg ttcacgttca ttaaaacagt gcgcgaaacg gcagcggcga
     8881 aaaggatggg gacggcgatg cggcagagtt tggggtagtc gagcttggta aagccgctgt
     8941 gccacagtcc ggcggtcagc cacaccatca tcacgccgcc catcatgcct ccgagggtaa
     9001 tcaggtgcag gggcgcggag gcaggtaagt tttgcagttt cgccgcgcct gtccacaaat
     9061 agcctgcggc ggcaaagagc tggagcaggt aataagtgcg gacgtagtgt ttgcgcagga
     9121 gttcgtgatg gtgcagttcg cgcagtttgg caagcaggat aaagccgacg gcgagcgcgg
     9181 taaaaccggc ggtttgcgcg ggcagccaaa gttcggcggc ggcgtgcaag agcaggaaag
     9241 taatggcgat gtttttataa acgatatttg gaataaaaac agggtctttc agacggcatt
     9301 ctttaagggc ttccgcgccc agcaaaacac tcacacgtat ggaaacaaac atcaccgcag
     9361 ccatatttaa atgcacttgc gcgcgcaaca ggttcaagtc gccggttgcc gcgtatgccg
     9421 tctgaaaaac ggtgaacgcg gcaagcagca tcagcagggc gaagttatcc gtgttgcggg
     9481 ccagccaaat caaccaagcg cagaacagca ataaaaccag ccaataagcc gctacaaaga
     9541 atgcggcaag ttgcggtaaa aacggcaata aaacagccgc aacaagcaac aacaccgcca
     9601 tcaaagtagt ggcaggtttc aggttgcccg aaaaacccgt ccagtccaac aaagccgcag
     9661 tcaggaaacc accatatgct gcaggcagca tgagttctaa gaagatttgg cggtgcagga
     9721 cgacgacacc ggggctgatg aaaaacacca acgcgccgag tatggcaagc actgtcgcgc
     9781 cgacgcaaaa cgggcgcata gcaacagtat ttttcacccc gtcgggcaaa aataccaaaa
     9841 ctcaaatcaa gccgtccgga tacc
//
LOCUS       DAWKST010000069         9798 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.069, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000069 DAWKST010000000
VERSION     DAWKST010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.069"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(13..1308)
                     /locus_tag="WJL87_001165"
     CDS             complement(13..1308)
                     /locus_tag="WJL87_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ7799592.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMEEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAIRSQHWEELQQWHEHAQKYYGATHYQL
                     WDNTELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGTKIFEHSPMIRIEP
                     YQNGWLVYTPEGSVECKNLVYAVNTYAGLNPIFQPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     DDVKIEYSWGGECDITANLVPHFGRLAANVFYAQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1434..2321)
                     /locus_tag="WJL87_001166"
     CDS             complement(1434..2321)
                     /locus_tag="WJL87_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ7799593.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2321..3286)
                     /locus_tag="WJL87_001167"
     CDS             complement(2321..3286)
                     /locus_tag="WJL87_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ7799594.1"
                     /translation="MNLNKLKNKLFRRPGQRVVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNITISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALMTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3302..4426)
                     /gene="potA"
                     /locus_tag="WJL87_001168"
     CDS             complement(3302..4426)
                     /gene="potA"
                     /locus_tag="WJL87_001168"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ7799595.1"
                     /translation="MTATTASSAKPYLQIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4622..4729)
                     /locus_tag="WJL87_001169"
                     /pseudo
     CDS             complement(4622..4729)
                     /locus_tag="WJL87_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4808..5077)
                     /gene="rpsO"
                     /locus_tag="WJL87_001170"
     CDS             complement(4808..5077)
                     /gene="rpsO"
                     /locus_tag="WJL87_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ7799596.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5300..6235)
                     /gene="secF"
                     /locus_tag="WJL87_001171"
     CDS             complement(5300..6235)
                     /gene="secF"
                     /locus_tag="WJL87_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ7799597.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTKGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6239..8092)
                     /gene="secD"
                     /locus_tag="WJL87_001172"
     CDS             complement(6239..8092)
                     /gene="secD"
                     /locus_tag="WJL87_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ7799598.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALQNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRVGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(8304..8570)
                     /gene="yajC"
                     /locus_tag="WJL87_001173"
     CDS             complement(8304..8570)
                     /gene="yajC"
                     /locus_tag="WJL87_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ7799599.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            8698..>9798
                     /locus_tag="WJL87_001174"
     CDS             8698..>9798
                     /locus_tag="WJL87_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ7799600.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKIFRL
                     DNDTAISTGSLSAAHFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2511 a         2585 c         2430 g         2272 t
ORIGIN      
        1 tgcctgcgac gcttaacggc tgtctttcca ctgataatat ttcgagccga ggaaagaacc
       61 gagtttgcgc aaaaacggtt gcaggataat attcggctgg cgcaaccgct caaacggctt
      121 cagacggcat tcgtccccta aaattgcttc ggcaaccgcc agacctgcaa tgcctgttat
      181 cgccatcccg tgtccggaat aaccttgtgc ataaaaaaca tttgcggcca aacgtccgaa
      241 atgcgggaca aggttggcgg taatgtcgca ctccccgccc cacgaatatt cgattttgac
      301 atcgtcaagc tgcggaaaaa ctttaagcat atcttggcgg acaagctcgg tcatacgcgc
      361 aggattgtcg ataaactcgt tatccttacc gccgaaaagc agtctgccgt ccgcgctgag
      421 gcggtaataa tccaaaatat ggcggttgtc gcatactgcc atattgttac ggataagccc
      481 ttttgcgcgc gcccccaagg gttcggtcgc aataataaag gtgctaacgg caatcgcctt
      541 gcgttccaaa ggctggaata tcgggttcaa acctgcatag gtattgacgg catacaccag
      601 atttttgcat tcgacgctgc cttcgggggt gtaaaccagc caaccgtttt gataaggttc
      661 aatgcgtatc atgggagaat gctcaaaaat cttcgtacca gcttcggcag cggcgcgggc
      721 gatgcccaac gtgtaattga gcggatggag atgcccggac aagggatcga actgtgcgcc
      781 ttggtacata tcgctgtcaa gctgctgctt cagttcagtg ttatcccaga gttgataatg
      841 agttgcaccg taatattttt gggcgtgctc atgccattgt tgcaattctt cccaatgctg
      901 cgaacggatg gcaaccgtgg cataaccgcg ctgccaatcg caatcaatgg catgtttgcg
      961 gacgcgttcg tccaccagtt cgaccgcctg caaagactgt tgccaaaacc attgcgcttg
     1021 ctccaaaccg acctgttttt caatttcctc cataccgcag gcgtaatcgc tgataacctg
     1081 cccgccactc cgtcccgacg cgccgaaacc gatacgcgcg gcttccaaca caaccgtttc
     1141 atgtccctgc tccgccaagg gcaatgcagt gcacaaacca ctcaatccgc cgccgatgat
     1201 acaggtatcg gttttcagac ggcattgaag tttcggataa acagtatgag gattaaccga
     1261 actgaaataa taagaaggca gatattcttg aaaatcaggg cgaatcattg tgtttgcttt
     1321 atcgggtata ttttcggacg gaatgataca ggctgtcgag ccatatcgtc caaacagaaa
     1381 atcggttgaa gaaaacaggc tgacccagtc atgcggtcag cctgccttat taattaattt
     1441 gctttctcgg cagccaattt ttcttggcgg taggcttctg ccgcttctcg gtcacgcttg
     1501 gttgcctgcc tcatcatcca ataattgacg atgatgacca atgttccgat gatgccgatc
     1561 aggatggttg ccaaaacgtt catctgcgga tcaagaccca gcttgatttt ggagaaaatc
     1621 acctgcggca atgtggatga accggggccg gagaggaatg aggtaatcac caaatcatcc
     1681 aaagacaggg taatgccgag cagaaagcct gaagcgatgg caggggcaat caaaggcaaa
     1741 gtgatgacaa aaaagatttt cagcggacgc gcgcccaaat ccattgcggc ttcttcgagc
     1801 gattggtcaa gctcaaccag acgcgaacgg ataacaacgg taatgtatgc catgcacagc
     1861 gtcgtatgtc ctaagaaaat agtgaaaaag ccacgatcga agtagagatg ttgtaaccat
     1921 tcactgccct gcaaaaatat ctgtacctga ataatcagca gcagcataga cagaccggta
     1981 atcacgtcgg gcatcaccat aggtgcggaa atcataccgg cgaacaaggt actgccgcga
     2041 aaacgtttaa tccgcgccat cgcatagcct gccagcgtgc ccaaaacgac ggcggcaagc
     2101 gaagacacaa cggcaatccg cagcgacagc caagcggctt ccaagatggt gtcgttttcc
     2161 agcaatgcgc cgtaccactt ggtcgaaaag ccgccccaaa cggttaccag cttggattcg
     2221 ttaaacgaat agatgaccaa aacaaccagc gggatataca gaaacgccag cgacagtgcc
     2281 aacatcagtt tcaagaacca agataatttg gatttctgca ttatttggct ccttcttcca
     2341 attcgcggtt ttcataatgc tgaaacaggg caatcggcac gaccagcagc gcgaccatca
     2401 cgacggcgac ggcggaagcc agcggccagt tgttttgatc gaagaacgcc tgccacaaga
     2461 ctttaccaat catcaggttt tccgaaccgc cgaccagctc gggaatgacg aactcgccga
     2521 cagcagggac gaaaaccagc atggagcctg caataatgcc ggttttcgac aaaggcaggg
     2581 taatcgtcaa gaacgatttg accggccccg cgcccaaatc ggaagccgct tcaagcaggc
     2641 ggttgtcgag tttcaccagt tgcgtgtata gcggcagaat cataaacggc agataggcgt
     2701 aaaccatcac caaattgagc gaaaaggcat tgtagaacaa atccaaaggc tcgctgataa
     2761 tacccatttt aatcaacagg ttgtttacaa taccgttatg cccgagcaga cccatccacg
     2821 catagacgcg caacaggaac gatgtccaaa agggcagcat aatggcaagc agcaaaccat
     2881 tgcggacaga aggattggca cgagaaatcg cataggcggt cggataaccg accaacaggc
     2941 aaatcactgt cgtcatcaat gcggttttga ttgaagacca gtaggtcatc agatagatat
     3001 tgctgttttc actgtcgccg aacgggttaa gggtatgcca aaaattttgg aaaatatcgg
     3061 catagttttg ataactgatg gtgatattca gacgacctaa atcttcgtcg atgctggtca
     3121 gcggcgtaaa cggcggaatg gcgatttctt gttcggcaaa gctgattttc agcacgatgg
     3181 cgaacggaat cagaaacagc accaaaagcc aaatatacgg tacggcaatc accacacgct
     3241 gccccgggcg gcggaacagt ttgtttttca gtttattaag gttcattgca ttccccttaa
     3301 atcaacggaa cagtggcgta ggctggtttt ccggccagct gatatacacg gtttcgtccc
     3361 aagttggcgg tgtaatgttg cgtacatacc agtaaggtgc aggaacttgg cttttgacga
     3421 cgcgcccgtt ggcgagtttg atatggtata tggcaaagct gcccaaatag gcgatttctt
     3481 ttaccgtgcc tttcgcccag ttgtagtcgc ccaaatattc gggtttttct ttatataaat
     3541 caatatcctc tggtcgaata ctaacccaaa ggtcctgctc gctcggacca cccaaaccgt
     3601 gatcgatgcg gacgtggttt tccaaacctt cgcattcgat aacggcatag tcggcatgat
     3661 cttcaatcac cacaccgtca aagatgttgg tttcgccgat aaactcggca gtgaagcggc
     3721 tgttgggata gtcgtacacg tcgctgggtg tgccgacttg ctgcaactga ccgtcagaca
     3781 taatggcgat gcgggtcgcc atcgtcatcg cctcttcttg gtcgtgcgta accataatac
     3841 aggttacgcc gacttgttcc agcgtattga ccaactcaag ctgggtttgt tggcgcagtt
     3901 ttttgtccaa tgcaccgagg ggctcatcca gcagtagaat tttcggacgt tttgccagac
     3961 tgcgtgccaa agcaatgcgc tgctgctgac caccagaaag ctggtgaggt ttgcgtttgg
     4021 caaatttggt catctgaacc aggcggagca tttcttcgac gcgcgcggcg atttcgcctt
     4081 taggcatttt gtcctgtttc agaccaaagg caatgttttg ttctacggtc atatgcggaa
     4141 aaagcgcgta actttggaac atcatattga tggggcggtc atagggtgca agtttggtaa
     4201 tatcctgacc atcaaggata attttcccct gattggggct ttccataccc gccagcatac
     4261 gcagcagcgt ggattttccg ctgccggaac tgcccaaaag ggcgaagatt tcgtgttgat
     4321 aaatgtccaa gtcgatgtta tcgacagcgt aattgtcacc aaactttttc accaagcctt
     4381 ggatttgcag ataaggtttg gctgaagacg cagtggttgc ggtcataatg gcaatactcc
     4441 aataaaaaaa cgagtaccgg caaaacggat tttcgaatgg gtgataaaaa gctgtttgat
     4501 tgctggcggg agtcgcaacg tctgatgccg tctgaaactc ttgtgaagcg cacgggcagc
     4561 atgaaatgga acaagattcc aaagaacttt atattatatt agtttatgcg gttttcgggc
     4621 aatatagtgg attaaattta aaccagtaca gcgttgcctt gccttgccgt actatttgta
     4681 ctgtctgcgg cttcgtcgcc ttgtcctgat ttaaatttaa tccactataa atatggattt
     4741 gagcttgtcg gaaagcaaca gaaaagaaaa caccgcccat ttttctgggc ggtgtcggaa
     4801 agcgtaatta tttacgcaga cccaagcggg taatcaacgc gcgatacgta tcgggctggg
     4861 tacggcgcaa gtaggccagc aggcggcggc gttggctgac cattttcaac aggccgcgac
     4921 ggctgtggtg gtctttgggg ttggctttga agtggggggt caggtcgttg atgcggaaag
     4981 tcaacagagc gacttgtact tcggaagagc cggtgtcgcc ttctttgcgt tggaaatctt
     5041 taacgatttg tgctttttgt tctacggtca gtgccataat gaaaactcca aaaatataag
     5101 aatccccata gggattcaga caagtttgcc aagcctgaag acaacggcaa actccctatg
     5161 cccaagatag gacaacgtgg cattatgaca caattcccgc atttctgcac aatattttaa
     5221 gaccgtggaa gtaaccgtta aaaatgccgt ctgaagcatt gtctgcttga gacggcaatg
     5281 ttcagacggc atatgcgctt caaaccacga tttcttcttt cttcttcggt tctttaccga
     5341 tattgtcgcg gctcaaaccg aacattagca gaagcgggct ggcaaccaat acggaagaat
     5401 aaatgccgaa cacgatgcca atggtcaacg ccatagaaaa gccgtgcaag gccgcaccgc
     5461 cgaacaccag catagaaaca accatcgcct cggtcgaacc gtgggtaatg atggtgcggc
     5521 tcatcgttgc ggtaatcgcg ttgtcgatga cttccggcac ggcatgtccg cgcatcgccg
     5581 gcttgcggaa gttttcacgg atacggtcga agacgacgac ggattcgttc acagaatagc
     5641 ccaatacggc aaggataccc gccaagacgg tcagcgaaaa ttcccattgg aagaaggcaa
     5701 agcagccgag aataatcacg atgtcgtgca tattggcgat aatggcagat acggcaaaac
     5761 gccattcaaa acgcatcgac aggtaaataa tgatgccgat aacgacaaaa cctaaagcca
     5821 tcaatccatt acttaccaat tcctcaccga cttgcgggcc gataaattcg acttggcgca
     5881 aggtaacgtc gggactgtct tttttcagca aatccataac ctgattggac aactgtgcgg
     5941 aagtaacacc ttctttgttc ggcaggcgga tcatgatgtg tttgttcgta cccaatgcct
     6001 gaacctgtac atcacctatt ttcagcgtat cgaggcgttc gcgcatctta ttgacatccg
     6061 caccctgctg atattggact tccattaccg taccgccggt aaattcgaca gagaaattca
     6121 ggcctttggt caccaagaag aacacggcag cgataaacgt aaccaacgaa atgaaggtcg
     6181 tcagtttgcc gtagctcata aacggaatat cgcgtttgat tttaaagagt tccatagctt
     6241 actccttacc tgccgcagtt tcggcttcag gtttccatac cgcaccgatg gaaatattct
     6301 gcaatttgcg tctgcgtccg taccacagat tgaccaacgc acggaatacg acgacggacg
     6361 aatacatcga agtcagaata cccaaacagt gtacgaccgc aaaaccgcgt accgggccgg
     6421 aaccgaatac caaaagcgcg ataccggcaa tcagcgaagt caggttggaa tcgacaatgg
     6481 tcgcccatgc gtgttggaaa ccgagattga ttgcctgctg cggcggcacg ccgacacgca
     6541 attcttcgcg gatacgttcg ttaatcaaga cgttggagtc gattgccata cccaaagtca
     6601 acgccagcgc ggccataccc ggtaacgtca acgttgcctg catagcagac aaaataccga
     6661 ttaggaacag tatgttggca ctcaatgcaa tggtagaaaa gaaacccatc agacgatagt
     6721 aaaccaccat gaatgcagca acgatggcaa aaccccataa agtcgaatgg aagccttttt
     6781 cgatgttctc cttacccaaa gacggaccga tggtacgttc ttcgacaatc tgcatcggcg
     6841 cggcaagaga accggcacgc aacagcaaag acgtatcatt ggcttcggct gtcgtcatgc
     6901 ttccggaaat ttccacgcgt ccgccggtaa tggcagtacg gataaccggc gcggttacaa
     6961 cctcggattt tccttggtcg atcaaaacca tcgccatgcg tttgccgaca tttgcggcag
     7021 tcagttcgcc gaaaatgctg ccgcccgcgc tgtccaagct cagactgacg gcaggtgcgc
     7081 ccatttggtc gaaactcggt tgcgcatcgt tgatgttgtc gcccgtcagc tcgacctgtt
     7141 tgctgattag cagagtttcg ggacgatctc cgccgcttga aagcagctca taaccgctcg
     7201 gcacgttgcc ttccaatgcc tcgcgcaact tggcaggatc gtcctccacc atacgcaatt
     7261 ccaaagtcgc ggtacggccg atgatgtctt ttgccttggc agtatcctga acgcccggaa
     7321 gctgcacgac gatacggtct gcacctgact gctggatgac gggttcggcc acgcccaact
     7381 cgttcacacg gttgtgcagg gtagtgatgt tctgtttgac cgcatcggaa cgcactttat
     7441 tgaccgcctc ttccgaaagc gtcaagacga tattgctgcc gtctgaattc agcgttgctt
     7501 caggaaacag cttgcgcaac tgcggcagtg ccttttgcac atcacctgca tcctgcaaag
     7561 ggacggtcag gctgtttcca gcctgacgca ccgtgccgct gcggattttt tcgcggcgca
     7621 gttcgcggcg gatgtcgccc gaataacgtt caaacgtttt ctgcatcgcc gctttcatat
     7681 cgacctgcat ggtgaaatgc acgccgccgc gcaggtccaa acccaaaaac atcggattgg
     7741 ctttgatttt cgccatccat tcggggctgt ccgccaacag gttgagcgcg gtaatatacc
     7801 cttcgcccaa agtgttttcg atgacgtcgc gcgctttaag ctgcgtttct gtgtctttga
     7861 aacgcacttt cagtgaattg tccacaacaa acatcccgtc ggtctgaata cccgcgtttt
     7921 gcagcgcggc atccactttg gattgagtct gttcgttgat gatgatggct tgtcggttgg
     7981 tcgatacctg cacggcgggt gtttcgccga ataggttggg cagcgaatac actgcggcaa
     8041 ccgcaatcgt gaacacaatc agcagatatt tccataaagg ataacggttc atcattgttc
     8101 cttaatggtt ggaaccccac cctttcggtg gtgtcggaat cgggctattt cagaagaggc
     8161 aaaaaccctt cccagccagg caagaccgga aggcggcatc ttgaatatgc cgccctgcgt
     8221 gtcggaacat ggtcaagcct tcggttggaa ttcaaaacaa agtgccgcat tcgggctttc
     8281 cagatgcggc ttgtcggcac aaatcaatcg acttttgcgg caatcgcatt gcgttctact
     8341 tcgacctcga tttttgtacc ctgtccgata tccacggtaa aaaactgttc gccgactctg
     8401 gttaccttac ccttgaaacc tgccgccaag accactttgt cgccgacttt caaggcggca
     8461 agcattgcct gatgcgcttt gaatttcttt tgctgcggac gcatgatcag gaagtagaac
     8521 accaccataa tcaacactaa aggagcaaat tgtgcaacag cttgattcat aatttatccg
     8581 ttctttctaa tatggttgaa aatcgagagg ggtatataat aacataagac cgtaaacaat
     8641 atatcgggtt tgccgtccgt accgaccgta taccgcagcc tgcccgtcca caaacccatg
     8701 tccttgaccc gcctaatctt gaaattctat gcactgttgc gccttttttt gggcaaaaac
     8761 gcccgcaccg catggatttc gcatcccgcc tgtgccgggc acgaacccgg cgcaaaccat
     8821 cccgattcgc ccgaccgcat cctctgcatc gagcaggcat tgcgccgcgc cggtatttgg
     8881 cagcacctcc aaaccataga ggcggaagaa atcagcgata cgcgcctcgc acttgtccac
     8941 tcgagcaaat atctgaaccg tttggaatct tgcctgcccc aaaaaggcaa gattttccgc
     9001 ctggataacg acactgcaat cagcacaggc tcgctgtctg ccgcacactt tgccgccggt
     9061 tcggcagttc aggcagtcga catggtcatg aaccgtaaag catggcatgc cttttgcgcc
     9121 gcccgcccgc ccggacacca tgcgggcagc ggcaaagccg gcggattctg cctgctgaac
     9181 aacgttgccg ccggcgtcat gcatgccatt gccgaatacc gcctgaaacg cattgccgtc
     9241 atcgatttcg atgtccacta cggcgacggt acggcagaaa tattcaaaga cgatccgcgc
     9301 atcctgtttt tcaacctgtt tgaaaccgac cttttcccct tccccgaaaa caacgatatg
     9361 cccgacggcg gcaatatggt gcacctgccc ttgccgccgg gaacgggcag ccgcacattc
     9421 cgcgaagccg tccgcaggca gtggctgccc cgacttgccg cattcaaacc cgaactggtg
     9481 ctgctgtcgg caggattcga cgcacaccgt ctagacgaat cgggcaggct caacctgcac
     9541 gaggcggatt ttgcctggct gacacacaaa atcattcaga cggcatcggg ctgccccggc
     9601 aaaatcgtat ccgtactcga aggcggctac acccttgaac ctctggcaca atctgctgcc
     9661 gagcatatcc gagtgctggc agggctgggc aaatccgatg ccgcaaccgc ctatcagaaa
     9721 acactggacc caacaaaaaa acggtttgcc aaaccgaaaa ccggacaggt gcgccaacct
     9781 acccaatccg accgttac
//
LOCUS       DAWKST010000070         9645 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.070, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000070 DAWKST010000000
VERSION     DAWKST010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9645
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.070"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..545)
                     /locus_tag="WJL87_001175"
     CDS             complement(<1..545)
                     /locus_tag="WJL87_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7799601.1"
                     /translation="MFCTVSHLRRLKGHDVLLTAFARALAQCPQLRLNIGGSGQEEQR
                     LKQQAADLGITHAVTFLGALQPEAVLDLMRNSDAFVLASRTETFGVVYIEALSQGLPV
                     IATRCGGAESIVSDGNGYLVPVDDDDALADALIKMYEHHSDFEPARLRENCLNEFGEN
                     AVIGRLIGIFRQAIAEYGKKIR"
     gene            complement(577..960)
                     /locus_tag="WJL87_001176"
     CDS             complement(577..960)
                     /locus_tag="WJL87_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7799602.1"
                     /translation="MYFFPRFPFIDIDCIRWVCAGLKAFKHYIRENGLPDLIHAHRMN
                     YAGILAQKISQKYGIPYVVTEHSSTITRGLIRPHQWQPMEKAAAHASALLAVSRHFAH
                     VLQHKYGCEWQYLPQHTGRNIQPTV"
     gene            1329..2033
                     /locus_tag="WJL87_001177"
     CDS             1329..2033
                     /locus_tag="WJL87_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ7799603.1"
                     /translation="MLRSILAASLLAVSFPTAAEALNYNIVEFSESAGVEAVQDTMSA
                     RFQVTAEGRDKNAVNAEFVKKFNNFTRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDALNRFIADVQADAALEYTDFHVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     PGVEEISISVNGTVQF"
     gene            complement(2097..2663)
                     /locus_tag="WJL87_001178"
     CDS             complement(2097..2663)
                     /locus_tag="WJL87_001178"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ7799604.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(2729..3145)
                     /locus_tag="WJL87_001179"
     CDS             complement(2729..3145)
                     /locus_tag="WJL87_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799605.1"
                     /translation="MLKHLAFLLPAMMFALPAASAVLTSYQEPGCTYEGDVGKDGKPA
                     GKGTWRCQDGRNYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            complement(3197..4096)
                     /locus_tag="WJL87_001180"
     CDS             complement(3197..4096)
                     /locus_tag="WJL87_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ7799606.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSIMLEELVSYLGGWQD"
     gene            complement(4127..5584)
                     /gene="der"
                     /locus_tag="WJL87_001181"
     CDS             complement(4127..5584)
                     /gene="der"
                     /locus_tag="WJL87_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ7799607.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEEIL
                     ENFPEPEVEEADAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENADDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(5734..6363)
                     /locus_tag="WJL87_001182"
     CDS             complement(5734..6363)
                     /locus_tag="WJL87_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ7799608.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELTKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFS
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(6364..7659)
                     /gene="hisS"
                     /locus_tag="WJL87_001183"
     CDS             complement(6364..7659)
                     /gene="hisS"
                     /locus_tag="WJL87_001183"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ7799609.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVNRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGERADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAEDLTHTLQQWKNA"
     gene            complement(7785..8327)
                     /locus_tag="WJL87_001184"
     CDS             complement(7785..8327)
                     /locus_tag="WJL87_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ7799610.1"
                     /translation="MQSWKARRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVL
                     KKLDKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFT
                     HHPKRQTEFAVRQIRQARAE"
     gene            complement(9444..9644)
                     /locus_tag="WJL87_001185"
     CDS             complement(9444..9644)
                     /locus_tag="WJL87_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799611.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
BASE COUNT         2254 a         2320 c         2463 g         2608 t
ORIGIN      
        1 cgtattttct taccgtattc tgcgattgcc tgtcggaaaa tgccgatcaa cctgcctata
       61 acggcatttt cgccaaattc gttcagacag ttttccctaa gtcgggcagg ttcaaaatca
      121 gagtggtgtt catacatttt gatgagtgcg tcggcaaggg catcgtcgtc gtcaacagga
      181 accaaatatc cgttgccgtc tgaaacaata gattccgcac cgccgcagcg tgttgcaatg
      241 acgggcaatc cttgggacag tgcttcgata tagactacgc cgaaggtttc ggtgcggctg
      301 gcaaggacga atgcgtcgct gttcctcatc aaatccaaga ctgcttcggg ctgcaatgcg
      361 cccaaaaatg taacggcatg ggtaatgccc aagtctgccg cctgctgttt cagccgctgt
      421 tcttcctgtc cgctgccgcc gatgttcagg cgcagttgcg ggcattgtgc caacgcccgg
      481 gcaaaggcag tgagtaggac atcgtgtcct ttgagacggc gaaggtgcga gacggtgcag
      541 aacacgaaat gcggtttgtt gtttttttct ctttgttcaa acggtcggtt gaatattccg
      601 cccagtatgt tgggggaggt actgccattc gcagccgtat ttgtgttgca ggacgtgtgc
      661 gaaatggcgg ctgacagcga gcagtgcgga ggcgtgcgcc gctgcttttt ccatcggctg
      721 ccattggtgg ggacggatca gaccgcgcgt gatggtgctg ctgtgttccg tgacgacata
      781 ggggatgccg tatttttggg aaattttttg ggcgagtatg ccggcatagt tcatgcggtg
      841 ggcgtgaatc aggtcgggca gcccgttttc gcggatgtag tgtttgaaag ctttcaaacc
      901 cgcacacacc cagcggatgc agtcgatgtc gatgaacgga aagcggggga agaaatatat
      961 gccgtgccat gcatagatgt ccaaaccgct ttgccgatat tttgcaaaac cgtaaggccg
     1021 gtcaggatgc ttgctgtttc tttccgcaag tagcggaaca tcggtgcaag cacggcggtt
     1081 ttgatgcctt tcctctgcaa tgccagtgcc tgattttgaa aaaaatcccg tccacatcct
     1141 gttcggattg cggataccat gaggggatga cgaggacgtg caagggttcg ggcatagtgg
     1201 gattccgtat cggaaaggcg gttattataa gacagacgca gaccgaatat ttaaattgtt
     1261 gccttacgct aatgcaattt agcgcgccga tatgctagat tggcggtttt ataagtaagg
     1321 atacggatat gttgcgttct attttggcgg cttccctgct ggcggtatct tttccgacgg
     1381 cggctgaagc attgaattac aatattgtcg aattttctga atcggcgggt gtcgaggcgg
     1441 ttcaggatac aatgtccgca cgtttccaag tgacggcgga aggacgggac aaaaatgccg
     1501 tcaatgccga gtttgttaaa aaattcaaca atttcaccag aaaatcaaaa aatggtagct
     1561 ttaaaaccga attggtatcg cgcagtgcga tgccgcgcta tcaatatacc aacggcagac
     1621 gcattcaaac aggttgggag gagcgtgcgg aatttaaggt cgagggtagg gattttgatg
     1681 cgttgaaccg ttttattgcc gatgttcagg cagatgccgc gttggaatat acggatttcc
     1741 atgtgtcgcg cgaacgccgc aacgaggtca tcgatcaggt cagcaaggat gccgttttgc
     1801 gtttcaaggc gcgtgccgaa aagttggcgg gcgttttggg tgcgtccggt tataaaatcg
     1861 tcaaattaaa tttgggacac atcggcagcc atatcgcggg agggggagct gctcaggcaa
     1921 aaatgcttcg tgccatgccg atggcggcaa gcgtcaatat ggagggtgcg gattccgccg
     1981 cgcctggtgt ggaggaaatc agcatcagcg tcaatgggac ggttcagttc tgatttgagg
     2041 tgaacggcaa atgccgtctg aaacccgacg ataagggttc agacggcatt tatatttcag
     2101 gctttgggca gggtaacgcc ggtttgcccc atatatttgc cgttgcggtc tttgtatgag
     2161 gtttcgcaga tttcgtcgct ctcgaagaag aggacttgcg ccacaccttc gcctgcgtag
     2221 attttggcgg gcaggggggt ggtgttggaa aattcgaggg taacgtagcc ttcccattcc
     2281 ggttcgaacg gggtaacgtt gacgataatg ccgcagcggg cgtaggtgga tttgcccaag
     2341 cagacggtca ggacgttgcg cgggatgcgg aaatattcga ccgtgcgcgc cagtgcgaag
     2401 gaattgggcg ggatgatgca gcagtcgtct tcaacggtaa cgaagttttt cgggtcgaag
     2461 tttttgggat cgacgatggt gctgttgatg ttggtgaaga ttttaaattc gtttgcgcag
     2521 cggatgtcgt agccgtagct ggacgtaccg taggagatga tgcgtttgcc gtcggcttct
     2581 ttgatttggt tcggctcgaa agggtcgatc atgccgaatt cttcgctcat tcggcgtatc
     2641 catttgtcgg acttgatgct cataatgttt tccttgtttc ttgcagtgtt cggacaaagc
     2701 attgggggat gccgtctgaa aacggggctt atttgttttt gggcagcttc acttctttaa
     2761 tcatgccgtt ttcgcatttc ataatgaaga gggtttcgcc gttttgcgag acggtaaatc
     2821 tgccgtgtgc caagcctttt ttgaatgtgc ccgagagtac catgttgcgg aatttggtac
     2881 tgtcggaatt gaacggttcg ataaatattt cgcggttggc ggcaacggta taaacgcctt
     2941 gtccgtcgaa tttgccattt ttaaacgaac cggtatagtt gcgcccgtct tggcagcgcc
     3001 atgtgccttt gccggcgggt ttaccgtctt tgccgacatc gccttcgtag gtgcagccgg
     3061 gttcttgata ggaagtcaga acggcggacg cggcggggag ggcgaacatc atggcgggca
     3121 gtaggaatgc gagatgttta agcataaggg ttattccatt ggattttggc tgacggtata
     3181 aaagccgtct gaaaaatcaa tcttgccagc cgcccaaata ggaaaccagc tcttccaaca
     3241 tgatgctgac ggattccgcc atcaggattt gcgaggcgaa ggcgagaccg gcggcatcgt
     3301 cgccgttgct ttcggcttct tcctgcaata cgtcgaggta ttggatgcgc ttgagtgtga
     3361 agtcttgagt gaggataaag gcgatttgtt cgcgccaaac caaaccgagc tgggttacgg
     3421 ttttaccgtt tttgacgtgt tgaaccactt cgtcggcggt taaatcttgt ttggatactt
     3481 tgacgacggg aacaatatcg cccgtgcctt tgagttcgca atcgctgtcc aattcaaaac
     3541 cgccttcgca atgcccttgc aacagccagc cggtcatcag ggaagagggc gattgtttgg
     3601 tattcggcag tgaggcttcc aaaccgccca aagcttcgcg cagcttggtc aggatgtttt
     3661 ctgctttggc ggaagccgcg ttattgacga gcaggtagcc gtggcgggtg ttaaacaccg
     3721 cttctgtacg gctgctgcgg gtaaacgctc ggggcagcag gtcgtctgta atttgctctt
     3781 taagctcttg tttttctttg cgaccgacat tgcgggcttc attgttttgg atttccgcta
     3841 ccttctcttc caaaatatcg cggatgacgc cggcaggcag gactttttcc tcttttttca
     3901 gggcgacgcg caaggtaaag tcggcaggaa aaacgagttc cggggagaat gaaaccggtg
     3961 cggtaaagcc ttcgctgaac cagtctaagc cttggcaatg ggtaaattca gcttcagcaa
     4021 gtttgtcggc aagtacgtcc gcctcaggca gcttttcttt gttgagcgga taaaaactaa
     4081 tctgcttgaa ccacataatg tttcctgttg tttgaaatgt cgggaattat ttgctgaatt
     4141 gttttttcac actgactttg gttttcttct tgaagcggtt tttctcttct ttgcggcctt
     4201 cgcgtttctc aatacggttg ctcaggctga cgcggcgcag cggttttttc ttcggtttgt
     4261 cgtcggcgtt ttcatacggg ttttctgaaa cattgtattg aatccgcaac ggcgtgcctt
     4321 gcaggttgaa ggctttgcgg aacgtttggg tcagatagcg cgtatagctg tcggaaatcg
     4381 cgtgcagcga attgccgtgt accacaatta cgggagggtt catgccgcct tggtgggcat
     4441 aacgcatttt cggacgcacc aagccggcac gcggcggttg ctgacgctcg attgcgcttt
     4501 gcaggacgcg cgtgattttc ggcgtcggca tcttaatcat cgccgcgttg taggcagcct
     4561 gaatgctgtc aaacaaaccg tctataccgc gctctttcaa tgcggaaata aagtggaact
     4621 tggcaaaatc gaggaaatac agtttgcggt tgatatcgcg tttcacttgc tcgcgacgtt
     4681 cttcgctgat gccgtcccat ttattgaccg ccaccaccaa agcacggcct gcctccaaag
     4741 cgaaacctgc aatcgtcgca tcttggtcgg cgatgtcctg ctgcgcgtcc aataccaaaa
     4801 cagcgacgtt tgccgcttca accgcctgca tcgctttgat aacggagaat ttttccactg
     4861 cttcatccac tttgccgcga cggcgcacac ctgcggtatc gatgatggta aacggtttgc
     4921 cttcgcgctc gaaatcgata tggatactgt cgcgcgtcgt gccggccata tcgaaggcaa
     4981 tcacgcgctc ttcgccgaga atggcgttta ccagcgtgga tttgccgacg ttcggacgac
     5041 cgatgacggc aaaaacggga tgttttgcat cggcttcttc gacttccggc tcggggaagt
     5101 tttctaaaat ttcttcaatc aggtaataca cgccgtcgcc gtgcgcaccg gaaataacat
     5161 aagggtcgcc caaagcaagt tcgtagaact cggcggcaag cacggccctg ttgccgcctt
     5221 cgcctttgtt taccgccaga taaacagggc gcgggctttg gcgcaaacgg tcggcaatga
     5281 ttttgtcttg cggggtcaga ccggtgcggc cgtccaccaa aaatacgacg gcatcggctt
     5341 catcgacagc ctgcaaggtt tgttttgcca tttcgtgcaa aatgccgctg tccacaaccg
     5401 gctcgaaacc tccggtatcg atgaccaaat aaggtttgct gccgactttg ccgtgtccgt
     5461 aatggcggtc gcgcgtcaga ccgggcaggt cgtgtacgag cgcgtctttg gtgcgcgtca
     5521 aacggttgaa taaagtagat ttgccgacgt tggggcggcc gacaagcgcg atggttggtt
     5581 tcatgattgg gtctttctgt gtcaagtgcc gttcgggaga actgaacaca agcaggtgtc
     5641 cgttgggaca cggcagatgg ttttacggga attgccgtag gatagtgttg tctgaaatgc
     5701 cgtctgaaga gagggtggca tttcagacgg catttatttc agcgaatcga gtttcatttg
     5761 aaccaattca cgaccgacag aatcttgagg cattttttct aaagcctgac cgtagttttt
     5821 taaggcttcc tggctttttc cctgtgcggc atagacatcg cctttggttt ccatcagcag
     5881 gggggagaag tccgcttcaa ccggcgtgtc gagcgcggca agcgcggcat cgtatttttt
     5941 ttgttgcaac aacacaacgc ccagacgctg cgccgccaac gcttgaatca ggctgtcttt
     6001 ttggttggac aacacccatt tcaaatggcc ttcggcaaca tcgtaacgct gcgcgtcaaa
     6061 ttcggttgcc gccgccatca gcgtggcttg ggcggcggaa atagaatgcg ggtagctttg
     6121 ttggagtttg gtcaattcgg cattaatttc gctttgcggg gctttgtttt gcgccttttc
     6181 cacgatgttt gccagcaccg ccgccgcttc ctgattttgg gaagccgcac ggttttggta
     6241 aaccgtgtat cccaagtagc cgagtgctgc caaaatcagc agggcaaaca gccatttgcc
     6301 cgtggttttc caaaaatatt taaagttgtc taactcttgt tgttcttcga gatgggctgc
     6361 catttatgcg ttcttccatt gttgtaaagt atgggttaaa tcctcggcgg cgacagtttg
     6421 ctgaccgtgt gcgccgttca tgtctttgag cgtaaccgta ccgttcgcca gttcgtcttg
     6481 cgcgacaatc agggcaaagc gcgcgccgct gttgtcggct tttttcattt gcgctttcag
     6541 gctttgatag ccggaatgct gcattacatt gaaaccttgc gcgcgcaagg cttgcgcgta
     6601 tttcatcacc tgcaagtccg ccctttcgcc ttggtgcatt gcatagacat caggcgcagc
     6661 gttcacttcc agcgagccgt attcgctcac caaaagcagc agccgttcga tgcccatcgc
     6721 aaagccgatg gacggcgcag gtttgccgcc aagttcctca atcaaaccat cgtaacggcc
     6781 gccgccgcac acagttgcct gcgcgccgag tttgtcggtc gtccactcaa aaaccgtctg
     6841 attgtaataa tccaaaccgc gaaccaagcg cggattttca atatattgga tacccaaacc
     6901 gtccaacatc gccttgaagc gtgcatagtg gttttgcgaa gcctcgccca aataatccac
     6961 caaacgcggc gcggcgttgc agatttcctg caaatcaggg tttttcgtat ctaaaacacg
     7021 caaaggattg gttttcagac ggcgtttgct gtcttcgtcc aatttatctt catagcgtgt
     7081 caaatattca accaacgccg cacggtgtgc cgctcgctcc tcacggttgc ctaagctgtt
     7141 gatttccaaa gtcaggtatt cgcggatacc caatttttcc cacaagtcgg cagacatcgc
     7201 gatgatttcc gcatcgatat ccggcccttc aaaacccaaa gcctcgatac cgacctgatg
     7261 gaattgacga taacggcctt tttgcggacg ctcgcggcgg aacatcggcc ccatatacca
     7321 cagcttttgc gggctgttgt acagaaggtt gtgttcgacc accgcacgca gacaagaagc
     7381 cgtaccttcc ggacgcaggc tcaaactcaa agaatcgttt gaatcggaaa aggtgtacat
     7441 ttccttgccg accacatcgg tttcctcgcc gatggagcgg acaaacaaac cggtttgctc
     7501 gacaatcggc gtacggattt gctgatagcc gtaagcgcgc gtccagcggt ttaccgtatc
     7561 ctcaaacgcc tgccaaaacg cagccgtcag tttgaaatct ttttgctcga caggcagaag
     7621 gtcgttcatg cctttgacgg attggatttt ttgtgccatt tcaagtaaga atgcttaaat
     7681 caaattgcgg gcgattatag cggattttta agggtttgtg aggttggggg tggtttgcgg
     7741 acgggtaaaa tgccgtctga aacagttttc agacggcatt tgacttactc tgcacgtgct
     7801 tgcctgattt gtcggactgc aaactctgtc tgccgcttgg gatgatgtgt gaaaaacaat
     7861 ttatttgaaa ttgtctcggc ttttttcggc acgaccgcca aaatcttacc tgccaaattt
     7921 ccctcacggg tttgccaagc atccaaaaac tgcgccctgc tcatcgaaac atgacccagc
     7981 gacgggtcgg caagcaaaac cgtattgccg tctataccgc gcaataccga gaaatgatca
     8041 tccttacggt atttcagata cacgatgacg gggattttca actgcgcgag ctgctcgaaa
     8101 gacagggcat agcctttcgc ttcaaaaccc aaatctggca taatgcgccg catatcctca
     8161 aacgacgcgc gcatctgctc cttatccagc tttttcaaca cttcttcttc cgtcagcgtt
     8221 tgcccgtaaa aattgttcaa aagcgtcgcc accgaagccg ccccgcagga aaaatccaaa
     8281 tcctgcttta caatattgaa atcccgccgc gctttccaac tctgcacttt gatttttccg
     8341 taaacaacag gattatcgtt aaacatcggt gcagcattca gacgataaga taaagaaacg
     8401 acaacacacg ccaacagaaa aacatatttg aacttcatca tattgtccac ataaagggca
     8461 gcctgaaaat ctttcaggct gcccttgtca aattattcct agctttcggc ttttttggca
     8521 aaccaaacaa tccgattacc cgcataatac tttccattta ttgaaatccg acaagccgcg
     8581 cccaaaaatg ccatgcactg tcgatttccg cagcaatctt tgtaccgttt tcttcaaatt
     8641 ccaaatattc acccaataat aaacttgaaa cagaacgcgt gggcagcaag tgcccaccct
     8701 acgcttattc aaataatttg attaaataaa gaataaagaa aatgcatcag gaacaaaatt
     8761 ataatctgcc acctgactca caccgctttt aaagtaaggg gcatcaaaat caaaaccgca
     8821 aaaaaaataa tttttgcatt gatttttaat agatttaaaa ttcgaatata gtgtttctcc
     8881 agatttaaaa agtttatctt tatctatata tttgatgctt tccctatcca aaataaatat
     8941 tcaaacatta aaaaatcatt acatgaccaa gccaaatcat aaatttgatt caaatgggta
     9001 tcataaagat aaaaataata aggtttggga acaggtaaaa tatttaatgg aaaaggagga
     9061 ctaattttct taatatctga tgactgatat ccatatcttt ctatattctt aaaaatttca
     9121 tctttcttaa gataacaaga cattctgaag ataacaagac attctgacaa tcacaacctg
     9181 ttcatcggat atattatcta tttgcatggc gcgtataaca cgccatgcct gattaaaatt
     9241 agtctccctt accttagaat ctattatctt ttccaaagat gaaaatgccc cttatcttga
     9301 actctccagt tagaacctgg aagtttcgta cctcttaaat gtgtattaat atttcttata
     9361 gtttcattaa aatgccacgc gctgccgatt tcaacggtaa ttttcgtacc gctttcttca
     9421 aattccaggt attcccccat tagctaacgc aaagaagcag acgccatttc ggcttcgtta
     9481 tgataaaccc gccttccgtt gatatagact tccgcccctg tccgctccaa attccaaaaa
     9541 ttccgtactg aaatttccat atcccgatat tgtgcagacc atgttttttc gaaggttttc
     9601 ataaaatttc ctatacctgt ccaatcggca catatcaatt gcatt
//
LOCUS       DAWKST010000071         9557 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.071, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000071 DAWKST010000000
VERSION     DAWKST010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9557
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.071"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            41..196
                     /locus_tag="WJL87_001186"
     CDS             41..196
                     /locus_tag="WJL87_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799612.1"
                     /translation="MHKLCLATSRSDDFIRKTAASGTISNRPTEDGILSTRPAAYAAE
                     EPLYQAS"
     gene            205..1254
                     /locus_tag="WJL87_001187"
     CDS             205..1254
                     /locus_tag="WJL87_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ7799613.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKGETRKTATQAY
                     GQKASDGIKPQNAPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWITNGWITVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANILK
                     WAEMLLPGEYRRKKA"
     gene            complement(1323..2864)
                     /locus_tag="WJL87_001188"
     CDS             complement(1323..2864)
                     /locus_tag="WJL87_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEZ7799614.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSIGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYGVTMYIMACLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(3157..4512)
                     /locus_tag="WJL87_001189"
     CDS             complement(3157..4512)
                     /locus_tag="WJL87_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ7799615.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVAGKISIYETRGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPARPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGTGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMKQNLRA
                     HTARFERQTQALQHYRPDVSVCKNNIVRLQTALPAAFSQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKDTRGQVIRNADVLKQGQKLHITFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            4709..5530
                     /gene="nadE"
                     /locus_tag="WJL87_001190"
     CDS             4709..5530
                     /gene="nadE"
                     /locus_tag="WJL87_001190"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ7799616.1"
                     /translation="MMRIFSTGGKMDTQAVITHIARWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRPTLLLDMPIRQHPDQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGIHQTAFDNQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            complement(5598..6071)
                     /locus_tag="WJL87_001191"
     CDS             complement(5598..6071)
                     /locus_tag="WJL87_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ7799617.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAGLLAYMQNELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTDNFQPPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            6369..6701
                     /gene="trxA"
                     /locus_tag="WJL87_001192"
     CDS             6369..6701
                     /gene="trxA"
                     /locus_tag="WJL87_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEZ7799618.1"
                     /translation="MSSELIVHTSDAAFEKDVLNADIPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            complement(6754..7701)
                     /locus_tag="WJL87_001193"
     CDS             complement(6754..7701)
                     /locus_tag="WJL87_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7799619.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPEEYEAWLAEVLEAVAFVTGFEPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRRFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            8029..9417
                     /locus_tag="WJL87_001194"
     CDS             8029..9417
                     /locus_tag="WJL87_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ7799620.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPTDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGRRGDHKPGKEGFGGKTRGEGFKKEGFKKDGFKKTGEGFKGKRKAGDS
                     FAGRGERRYKDR"
BASE COUNT         2333 a         2660 c         2533 g         2031 t
ORIGIN      
        1 ttgaaagatt ctacccccgc acgcaaaccg atttcaaccg ttgcacaaac tttgcctcgc
       61 cacaagccgc agcgacgatt tcatccgcaa aaccgccgca tcaggtacaa tatcgaaccg
      121 tccgactgag gacggcattt tatcaacccg tcctgccgca tacgccgcag aagaaccgct
      181 ttatcaagcg agttaggaaa aatgatgtcc aaacagccca ccagcaaacg ccaatggcgc
      241 gacggcgcag ccccgtctgc caagaaaacc gccaaacctt tcaaaagcaa agcccgtccc
      301 aaaggcgaaa cgcgcaaaac cgccacccaa gcttacggac aaaaagcttc agacggcatc
      361 aagccgcaaa acgcccccaa acagcgcgcc gccaaagcca aaaaactcgt cgtccgcaat
      421 cccaaccaaa aaattatgga acacgcgcgc gatttgaaag aacgccgcag cgacctgtcg
      481 cgcatggaac ccgaacgcct gcaaaaagtg cttgccgcgt ccggcgtcgg ctcgcgccgc
      541 gaaatggaag aatggattac caacggctgg ataacggtca acggcaaaac cgcgcaactg
      601 ggcgacaaag ttacccccga cgaccacgtt accgtcaaag gcagcatcat caagctcaaa
      661 tgggcggacc gcctgccgcg catcatcctg tattacaaac aagaaggcga aatcgtttcc
      721 cgtgacgacc cgcaaggccg cgtcagcata ttcgaccgcc tgccgcaggc cgccagcagc
      781 cgctgggtcg ccatcggacg cttggacatc aacaccagcg gacttctgat tcttaccacc
      841 tccggcgaac tcgtccaacg tttcgcccac cccagcttcg aagtcgaacg cgaatacgcc
      901 gtgcgcgtct tgggcgggct gaccggcgaa caaatgcgcg tcctcaccga agaaggcgtg
      961 atgctcgaag acggcttggc aaaagtcgaa cgcatccgcg aacaaggcgg cgaaggcgcg
     1021 aacaaatggt acaacgtcgt gattaaagaa ggccgcaacc gcgaagtgcg ccgcattttt
     1081 gaaagccaag gactcaccgt cagccgcctc gtgcgcgtcg gcttcggtcc catcggactg
     1141 cccaaccgcc tcaaacgcgg gcagttctac gaactcaacc ccgccgaagt cgccaacatc
     1201 ctcaaatggg cggaaatgct gctgccgggc gaataccgcc gcaaaaaagc ctaaacccgc
     1261 caaaacacaa aaatgccgtc tgaaacatct gctgtttcag acggcatttt attcgggcgt
     1321 tttcaggaga aaaggtcgag tgctttgaca aagaccatca ccacgccgta ggcgagtggt
     1381 atgacggcca atgcccaacg ccaccagacc gatatgccgc cgccggaaac tttttcgccc
     1441 acaaggtaag cgtcggatat ggcggtttcg tcgtcggggt tgccgctgtg tgcggcggtt
     1501 ttgatgtctt tttcgtggtg tttttcgtgt acggatttga cggcgaggtt gcacaacaaa
     1561 ccgataatca gcaggcacgc catgatgtac atggttacgc cgtatgcctg tgccgccggt
     1621 atgccgctgt cgatttggct ttggcgtatg taattgacca gtaccgggcc gatgacggcg
     1681 gcggttgacc aggccagcag gatgcgcccg tgaatcgcgc cgacctgata ggtgccgaac
     1741 aggtctttca ggtaggcggg aatggcggca aatccgccgc cgtacatgga aataatcacg
     1801 caaaagccga tgatgaacag ggctttgctg ccgccctcgc cgatggaggg aacggcgaaa
     1861 tacagcagcg agccaagtac gaagaagatg gtgtaggtgt ttttgcgtcc gattttgtcg
     1921 gaaacgctcg accacaaaaa gcgtccgccc atgttaaaca ggctcaggag gctgacgaag
     1981 cctgccgccg cacctgcgcc gactgctgcc tgcctgccta tggaggtttc ggaaaagagt
     2041 tcctgaatca tcacggatgc ctgacccaat acgccgatgc cggcggttac gttcaggcac
     2101 aatacccaga acaacagcca aaactgcggc gttttcatgg cttgggacac gttgacatga
     2161 ttactgctga ccagcttgtt ttgcgttttc ggcgcggtat agccttcagg tttccagccg
     2221 tcggcaggta cgcggatggt aaacgcgccg aacatcatca gtgcgaggta aagcaggccc
     2281 aatacggcaa aggtttcggc aaccccgacc gaagcggcgt ttgaaaaggc gttcatcagt
     2341 gatacggaaa gcggagaggc cagcattgcg ccgccgccga aacccataat cgccaaaccg
     2401 gtcgccatac ccggcttgtc gggaaaccat ttcatcagtg tggaaaccgg cccgatgtag
     2461 cctaagccta agcctacgcc gccgatgacg ccgttgccca aatagagcag gaagaggttg
     2521 tgcgtacgca cgccgaatgc ggatacgaag aagcccaggc tgaaacagca ggcggcggca
     2581 aatatggctt tgcgcggacc tacccgttcc atccatgtgc cgaacagggc agccgacgcg
     2641 cccagcatcg cgagtgcgat actaaaaatc caacctacgg tcgtcagctt ccaatctccg
     2701 gccgccgatt cggttatgcc gataagtttg gtcagcggcg cgttgaatac ggaataggcg
     2761 taaatctgcc cgatggcaag gtgcaccgcc aatgctgcgg gcggtacgag ccaacggttg
     2821 aaacccggct tggcaatgct tgcctcacgg tctaaaaact tcataacatc ctctttctgt
     2881 cagttgaaaa ataaaatttc atttgcccaa tggaaactta ttgaaaatta taaaaaaata
     2941 tcggatcggt tttttatccg ccccaagatg ccccgtctga aacatttcgg gtgtacggaa
     3001 aggtttctgt tttttccgac aaattcctgc ggcttttcgc ttccggattc ccgctttttc
     3061 aggaatgacg aattaaagat tatcttaagg tcaagggact ggattcccgc tttcgcggga
     3121 atgacggcgg agggggagcg gtttttccga ttgggtttaa atgcaatcga acaaatcctg
     3181 ctgcccttgt tctttgctta cgcgcacgtc ggtttcgccg tcggcgaagg taatgtgcag
     3241 cttctgcccc tgcttcaaaa catcggcgtt gcggatgact tgtccgcgtg tatctttgac
     3301 gacggaaaag ccgcgttcca aaatgtgctg cggcgaaacg gcttcgagca atgcggcttg
     3361 ggcggtcagg ctttggcggc ggtgggtaag cagttggctg aaggcggcgg gcaatgccgt
     3421 ctgaaggcgg acgatgttgt ttttgcaaac ggaaacatca ggacggtaat gttgcagggc
     3481 ttgggtttgg cgttcaaaac gggcggtgtg ggcacggagg ttttgcttca tagagtaaga
     3541 cagcgtttgc gccagcttgc cgattgaagc gcgctgctca tcaagttttt ggcgcggatg
     3601 acggatttgc cgcgcaagcc agtcgagttt ttggctggca tcgaaatagc gttgttccaa
     3661 aacggttttc agacggcctt gcgcctgagc gagacgatgt agcgattctt ggcggttggg
     3721 gctgaccagc tccgccgcac cggtcggcgt gggcgcgcgc acgtcggcga caaaatcggc
     3781 aagcgtgaaa tcggtttcgt gtcccacgcc actgacgacg ggaaccgtgc aggcttcgat
     3841 tgcgcgcacg accggttctt cgttaaacgc ccacaagtct tcaatgctgc cgccgccgcg
     3901 acagacaatc aacacgtcgc attcggcgcg ttgcgaggcg gttttaatcg cttgtgcgat
     3961 ttgtaactcg ctgcctgtgc cttgaacagg tgtcggataa acgataacgg ggatttcggg
     4021 tgcgcggcgt ttcaaggtag taacgacatc gcgcaaagcc gccgccgcca gactggttac
     4081 gatgccgata cattgcggtc gggcgggcaa aggtttcttg cgttccgccg caaacgcgcc
     4141 ttccgcctgc aactgcgcct tcaaccgctc gtaggcttcg taaagctgcc ccaagccctt
     4201 gagccgcact tcgttcacgg taatctgaaa ttcgccccgc gtttcataaa tactgatttt
     4261 gcccgccact tcgatatggt cgccttcttt caaaggtttc gccaaacgcg ccgccgcacc
     4321 cttgaacatc gcgcaacgca cctgcgcgcg gctgtctttg agcgagaaat aataatgccc
     4381 gctggcggca cgggtcaggt tggacacttc gcccgcaatc cacagaccgg caaggtggtt
     4441 ttccaaaata cttttggcaa atgcgttcaa ctcggaaacg gacaacacgt cagaatggaa
     4501 aaaatcagac atcgaatcaa tcaaatagta aaaaatatga atatgttttg aagcccaagg
     4561 cggcatagag ccgcctaaat tgtcaacaat attataacac gcgccatctt gccgcccgcc
     4621 ttttcccata tgactttttt aagcggggaa tgggaaaaat attcatcaac ctgcctgcaa
     4681 tctattcaaa ttgaaccgcc gacaagctat gatgcggata ttttcgacag gaggaaaaat
     4741 ggatacgcag gcagttatca cacatatcgc ccgatggttg gacgaatacg ccgcacgggc
     4801 aaatgcaaaa gggtttgtcg tgggcgtttc cggcggcatc gattccgccg tcgtctccgc
     4861 actcgccgcc cgcaccggcc gccccacgct gcttctggat atgccgatac gccaacaccc
     4921 cgaccagctt gagcgggcaa ggctgcacat ccgcaatctg caacggcaat atgccaatgt
     4981 aagcgcgcaa acggtcgacc tgaccgacac cttccagacc tttgaacaaa ccgtcggcat
     5041 tcatcagacg gcatttgaca atcagccgct ttccctcgcc aacgccagaa gccgcctgcg
     5101 tatgctgacc ctgtactact acgggcagat acacggactg ctggttacgg ggacaggtaa
     5161 taagattgaa gatttcggcg tgggcttttt tactaaatac ggcgacggcg gcgtagacat
     5221 cagcccgatt gccgacctga ccaaaacgca ggtttaccgg cttgccgaag cattgggcgt
     5281 ggacgaggcg attcaaaaag ccccgccgac cgacggcctg tgggatacgg aacgcaccga
     5341 cgaagaacag atgggcgcaa gctatcccga actggagtgg gcaatgggcg tgtacggcac
     5401 gcgcaaaccc gaagattttg aagggcggca gcgcgaagtt ctggaaatct atacgcggct
     5461 tcaccgcgcc atgcagcaca aaatcaaccc gattcccgtg tgccgcatcc cgcccgaatt
     5521 gttgggctga acgtgaaaat gccgtctgaa gcggaaaacc gtgtttcaga cggcatggaa
     5581 atatccggac gcgctcctta agaatcgagt acgcgggcaa acagaatatc gttttccaaa
     5641 tgaatgtggt cgttcaaatc ctccaccatt tctttcgcca gcgcgtaaag ccgcgtccag
     5701 cttccgcaag ccccttctgg cggttggaaa ttgtcggtca gctctttgag ccgtgcgatg
     5761 gcgcggtcgt gttcttcgtg ttcgtgcatc atcacgctga tgggcattgc cgcaccgcgt
     5821 ccgacaccct gattaatcat cggaaacagc atcctttcct ctttcatcat atgcatcagc
     5881 agttcgttct gcatataggc aagcagcccg gcaatttccg ccggaaaggt atcggcatgg
     5941 acttgggcca ctttctgagc cagcggcacc aattcttcaa attgtgcgcg gtggacattg
     6001 tggtagcgtt gcaggatatg atcgacggtt gcaccaaagg gggcggtctc ccaaacggaa
     6061 aaatcagtca tcgcagtgtt ccttttacag ggtttcgggt ttggttttga acattcatac
     6121 tttaagaatc aattcaaacg gagcatacac cgcccgcgcg cttctgtaca gcctcaaacg
     6181 tattccttac attttgataa taaaagtaat tttcagaaat aaaatactgt ccgaaccgtt
     6241 ttttagaatt tgcaaaggcg attggggcgg tacagaaaaa ctattatccc gcccgcccac
     6301 ttgaaatttt tatgcccaag ccctatcctg cacgctatcg tgccaatccc aaccgaaaag
     6361 gaaaaataat gagcagcgaa ttgattgtac acaccagcga tgccgctttt gaaaaagacg
     6421 ttttaaatgc agatatcccc gtcctgctgg acttttgggc tccgtggtgc ggcccctgca
     6481 aaatgattgc cccgattttg gacgacattg ccgccgaatt tgaaggccgt ctgaaagtgg
     6541 tcaaaatcaa catcgacgac aacgaagcca ccccgtcccg tttcggcgtg cgcggcattc
     6601 cgaccctgat ggtgttcaaa aacggcgaag tcgtcgccac caaagtcggc gcattggcaa
     6661 aaggtcagct gaccgccttt gtcgaagcct ctatcgcctg ataaagcgca atcgaaaaag
     6721 ccgccggaag attccggcgg ctttttcgca cccttaagat ttgtggcgga tttcccagca
     6781 cctatggatt tttttgttgc ggaaatcttc gggaacggat tgtttggaaa tgtctttgac
     6841 ggcgtattgt tccgatacca agtcgtctaa gacgaagctg cgcaggttgt tggaaaagta
     6901 caaaatgccg tctgaagcga gcagcttcac cgcgccgtca atcagctttt tgtggtcgcg
     6961 ctggatgtcg aggatgtcgg acattttctt gctgttggaa aaactgggcg ggtccatcac
     7021 aatgaggtcg aaccgcctgc cttccccata tgccgtctga agatattgga acacgtcggc
     7081 gcggacgatt ttatgccgtt ccgtatcgat gccgttcaat tcaaaattgc gtctcgccca
     7141 atcgagataa gtgttagaca aatcgacagt ttcgctggat gccgcgccgc cggtggcggc
     7201 atagacagtg aagctgccgg tgtaggaaaa caggtttaaa aaacgtttgc ccgccgccgt
     7261 ttcgccgact tttttgcgcg tgtttcgatg atccaaaaaa agccccgtat ccaaatactt
     7321 atcaaggttg acccaaaact tgcggccgtt ttcggtgatg acgaaatcgt cgcccgcctt
     7381 gccggttttt tcgtattgct gcaaaccttt ttggcgttcg cggcgtttga ggcggatttg
     7441 ttcgggttca aaaccggtaa cgaaagcgac ggcttccaag acttcggcaa gccacgcttc
     7501 gtattcttcg ggccgcatca gccagccggt atcgtattcc tgaaggtgga ttcgatcgcc
     7561 gtaaacatcg gcggcaaagg ggaattgggg gatgtcgcgg tcgtaaatgc gccaggcttc
     7621 aatgccgttg cgtttcgccc atttcataag gtgtttgatg tttttgccca agcggttggc
     7681 aaacggtgtg atgtcggtca ttggtttcag gcggaataaa gtggaaaacg gcaattttac
     7741 tgtaattaac gcccgattgc ttgaccgttt cgggcaaacc ctataccatc cgccgcttat
     7801 cttgtcatac gaagccatcg ccttccaacc taaaccgccc ttacgggcgc gtttcttttg
     7861 ttgctttgat tttgcaaagc ctttctgtgc aggttgtcgt cgatgtaaac cacaagcaag
     7921 ccgcttgcga caaccctgta acttcacatt ccccgtatcg ttacccttcc ctgcttcagg
     7981 ccgtctgaac ctttcggacg cgggcgttgt tgtcttccaa ggatagccat gtctattaaa
     8041 tttgccgatt tgaaccttga taaaaacatt ttgtccgccg tcagcagcga gggttacgaa
     8101 agcccgacgc cgattcaggc gcaggcgatt ccgtttgctt tggaaggccg cgacatcatg
     8161 gcttcggcgc aaaccggctc cggcaaaacc gccgcctttc tgttaccgac tttgcagcga
     8221 ctgaccaaac gcagcgaaaa accgggcaaa ggcccgcgtg ctttggtgtt gaccccgacc
     8281 cgcgaattgg cggcacaagt ggaaaaaaac gcgctggcgt atgccaaaaa tatgcgctgg
     8341 ttccgtaccg tcagcatcgt cggcggcgca tcgttcggct accaaacccg cgccctgagc
     8401 aaaccggtcg atctgattgt cgccacgccg ggccgcctga tggacctgat gcaaagcggc
     8461 aaagttgatt ttgaacgttt ggaagtgctg attttggacg aagccgaccg tatgttggat
     8521 atgggcttta tcgacgacat cgaaaccatc gtcgaagcca ccccgaccga ccgtcagact
     8581 ttattgttct ccgccacttg ggacggcgcg gtcggcaaac tggcacgcaa actgaccaaa
     8641 gaccctgaaa tcatcgaagt cgaacgcgtg gacgaccaag gcaaaatcga agaacaactg
     8701 ctgtactgcg acgatatgcg tcacaaaaac cgcctgctcg accacatcct gcgcgatgcc
     8761 aatatcgatc aatgcgtgat tttcacgtcc accaaagcga tgaccgaagt catcgcggat
     8821 gaattgtacg aaaaaggttt cgccgccaac tgcctgcacg gcgacatgcc gcagggctgg
     8881 cgcaaccgca cgctgatgga tttacgcaaa ggccgctgca aaattttggt tgccaccgac
     8941 gttgccgcac gcggtatcga cgtaccgacc attacccacg tcatcaacta cgacctgccg
     9001 aaacaggcgg aagactacgt ccaccgcatc gggcgcaccg gccgcgcagg ccgcacgggt
     9061 attgcgatta cgtttgccga agtgaacgaa tacgtcaaag tccacaaaat cgaaaaatac
     9121 attaaccgca aactgcccga actgaccatc gaaggcatgg aaccgacccg caaacgcaaa
     9181 tccgcaggcg gtaagccaaa aggcaaaggc ggctggggcg ataggaaatc cggcggccgg
     9241 cgcggcgatc ataaaccggg caaagaaggc ttcggcggca aaacgcgcgg cgaaggtttc
     9301 aagaaagaag gctttaagaa agacggtttc aaaaaaaccg gcgaaggctt caaaggcaaa
     9361 cgtaaagccg gcgattcttt tgcaggcaga ggcgaacgcc gttacaaaga ccgctaagcc
     9421 ccaacctgcc tcataaacca atgccgtctg aaaccgattt cgagtttcag acggcatttt
     9481 tgcaatgttt caacaccgcc cggctttgat acccaaagga ttaatctgca atatagtgga
     9541 ttaacaaaaa ccagtac
//
LOCUS       DAWKST010000072         9435 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.072, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000072 DAWKST010000000
VERSION     DAWKST010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9435
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.072"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(135..1577)
                     /gene="aldA"
                     /locus_tag="WJL87_001195"
     CDS             complement(135..1577)
                     /gene="aldA"
                     /locus_tag="WJL87_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ7799621.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRERADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVVAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVST
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1612..1812)
                     /locus_tag="WJL87_001196"
     CDS             complement(1612..1812)
                     /locus_tag="WJL87_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799622.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTDANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTAIYLGIN"
     gene            complement(1821..2210)
                     /locus_tag="WJL87_001197"
     CDS             complement(1821..2210)
                     /locus_tag="WJL87_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799623.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLIN
                     YSYLDNCLVTLTADRISVSFIREIQAA"
     gene            complement(2418..3335)
                     /locus_tag="WJL87_001198"
     CDS             complement(2418..3335)
                     /locus_tag="WJL87_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7799624.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRCRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWDLPPNLRGLAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFAGGA
                     HQVNRLTDALNISERTLQRRMRDHFGITTSEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            3508..4308
                     /locus_tag="WJL87_001199"
     CDS             3508..4308
                     /locus_tag="WJL87_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7799625.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILKNINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            4356..5132
                     /gene="mlaE"
                     /locus_tag="WJL87_001200"
     CDS             4356..5132
                     /gene="mlaE"
                     /locus_tag="WJL87_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ7799626.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            5183..5677
                     /gene="mlaD"
                     /locus_tag="WJL87_001201"
     CDS             5183..5677
                     /gene="mlaD"
                     /locus_tag="WJL87_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ7799627.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5714..6304
                     /locus_tag="WJL87_001202"
     CDS             5714..6304
                     /locus_tag="WJL87_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ7799628.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVSQIRQNATQVLSILKN
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGSK"
     gene            6361..6639
                     /locus_tag="WJL87_001203"
     CDS             6361..6639
                     /locus_tag="WJL87_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ7799629.1"
                     /translation="MHTELNNGTLHISGDITVKTLTGEAFERFQQQCRLKETIAVDFG
                     GVKRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6636..7463
                     /locus_tag="WJL87_001204"
     CDS             6636..7463
                     /locus_tag="WJL87_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ7799630.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            7588..7950
                     /locus_tag="WJL87_001205"
     CDS             7588..7950
                     /locus_tag="WJL87_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ7799631.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            7947..8333
                     /locus_tag="WJL87_001206"
     CDS             7947..8333
                     /locus_tag="WJL87_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ7799632.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            8330..8818
                     /locus_tag="WJL87_001207"
     CDS             8330..8818
                     /locus_tag="WJL87_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ7799633.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8902..9069)
                     /locus_tag="WJL87_001208"
     CDS             complement(8902..9069)
                     /locus_tag="WJL87_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ7799634.1"
                     /translation="MEDDVWPGRRVIVMAGVTVGRGSVVGAGAVVTKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(9036..9416)
                     /locus_tag="WJL87_001209"
     CDS             complement(9036..9416)
                     /locus_tag="WJL87_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HEZ7799635.1"
                     /translation="MGSKFFFLLLRFAGSVLPPSHMRGIGIVGRRVRGFLARRISPHI
                     GRGVNIERGAYVFPDTVLGDGSGIGANCEICRGPVVGKNVMMEPECLFYSNNHKFDRS
                     KNALRATRKSVRLRWRTMSGRGAG"
BASE COUNT         2177 a         2628 c         2385 g         2245 t
ORIGIN      
        1 ttaaatttaa tccgctatac aaagacaaaa acatcgacct cggtcatgac tgattgccgg
       61 tgaagcaata aaaatgccgt ctgaacctga aaacgggttc agacggcatt ttctatcggg
      121 gttttaaagc ggcattaaat gtcggtttcc aaataaacga cttgggtttg cagatattct
      181 tccaaaccgt gtttgccgtc cgcaccgccg atgccggatt ttttccaacc ggcgtggaaa
      241 ccctgcatcg cttcaaagtt ttcgcggttg atgtaggttt cgccgaattg caggcggcgg
      301 gtaacgtaga aggcttcgtt taaattagtc gtataaacag aactggtcag accaaactcg
      361 caatcgttag ccagtgcgat gacttggtcg agcgtgtcga aagtggaaac aggcaggacg
      421 gggccgaagg tttcttcttt cataatgtcc atactgttgt cggtgtcggt cagcagggtc
      481 ggctcgaaga aatagccgcg tccttcggcg cgtttgccgc cgcaaaccaa tttcgcacct
      541 tgtttgactg cccgttccac tttttcggca acggctttga cggcgcgctc ttcaatcagc
      601 ggacccattt ccagcgcgcc ggcttcggct tcggcagggt tgccgtagcg cacgcctttc
      661 atcgcggcgg tcattttttc aatgaatgcg tctttcaggc tgctgtggac atagacgcgc
      721 tcggcgcagt tgcagatttg accggtgttg ccgacgcgcg aagccaagat ggatttcact
      781 gccaaatcca aatccgcgtc tttcaaaacg atggcagggg ctttgccgcc gagttccaac
      841 gaaactttgg tgatgttggc ggaggcggct tccatcactt ggcggcctgc ttcgacggag
      901 ccggtcaggc tgaccatatc gacttgcgga tgggcggaca aggcattgcc gatttccgca
      961 ccagggccgt tcactacgtt gaacacgcct gcgggcagtc cgaccgcatc gacgatttcg
     1021 gcgaagatgt ggcagttgat cggggttacg ctgctgggtt tgacgacgat ggtattgccc
     1081 gttaccaaag ccgggcccat tttgcgggca atcaggaaga aggggaagtt ccacggcaaa
     1141 atgcctgcca cgacgcccag cggacgtttg aacaataaaa tgttttcgcg cgggcggtcg
     1201 ctttggatga tttcgccttc gtaacggcgc gcccattcgg cttggtaatc gagataatcg
     1261 gcagtgaaca tgacttccac gcgcgccaag tctttggttt tgccgccttc ggcaacgatg
     1321 gtgtcggtca gctcgtcggc gcgttcgcgt atgccttggg cgattttgcg caaatacgcg
     1381 ccgcgttcga ccgcaggcag acgctcccaa gccggttgcg ccgcacgcgc cgccgcgacg
     1441 gcgcggtcaa cgtccgcctt gccgcctttg ggttcgcggg cgatggtttc ttcggtggat
     1501 gggttcaata cgtcgcgcca ttcgccgttg aaatcgtttt caaagcgtcc gttgatgtac
     1561 atggccaatt gtttcatttc gggttctcca gttttgtagt cagatgtagt cttagtttat
     1621 tcccaaataa attgccgtac aagtttcttt tgaaatgttt agacgagaag ctactatgtc
     1681 tgcatattgt tctgctaatt tatattcact cgaatgcaac tgccgattat tccaatctgc
     1741 attcgcatca gtaaaagccc cagcattgcc gcctgtggca taaccaatcg ctaaactgcc
     1801 aacggtatcc acaacaggtt tcaggctgcc tgaatttctc taataaatga aacacttatt
     1861 ctatcagccg ttaatgtaac caaacaatta tcaagataag agtagtttat taaatactca
     1921 tcttcttcaa attgtatatt ttgtagggtt agatgattat tttttaattc tgccgttatt
     1981 tcatacttga aactatcatc tatttttcta aatccaatat ttgaagttga tttatccagt
     2041 atgtaatcat taaagatttc ataggataat tccacttcat aaaattgatt ttcaaatatt
     2101 ttgaatgggc agtacgatat aaaacaatgt agttcataac catttatttc aatcaatact
     2161 gcctcttcaa tggtgttatc taactttttg attttacatt tatataacat ttatcttgcc
     2221 tttggataaa ttgttttgtt ttttggattg ttattcaaag ttctccaaaa gtcttttcct
     2281 gattttgtat gtatcgtggt aatactacca ttttcattgg ttccaacaaa aataaattta
     2341 gattgacctt tggaatcagt acccaaaaac tgaacatatc cagacacgcg gatttcgggt
     2401 tttaaatcaa atgcggctta ccggttttct tttttggttt ccgcaggcgt gctgccgaaa
     2461 tattgcctga atgcctgagt aaagctggaa acgtggcggt agccgcataa atatgcggtt
     2521 tcgcctatgc ttttgccccc gttttgcaac agataaagcg cgtgctgcat ttgtttgtgg
     2581 tgcagccact cgcttgtggt aatgccgaaa tggtcgcgca tacggcgttg cagcgtcctt
     2641 tcgctgatgt tcagcgcgtc tgtcagccgg ttgacttggt gtgcgccgcc ggcaaacgcg
     2701 gcattcaggg tgcggctgaa gtcttcagac ggcatagcgt ctgcttccgc cgtttgcccc
     2761 gccgccggcc cgatgccgtc tgaaaccgtg tcccacaagt ccgacagcag ccgcaacacg
     2821 tccgcctcgc ggcgcaatgt ttcgcccaaa tgcccctttg ggacggcttt caggcaggat
     2881 gccgccaagc cgcgcaggtt cgggggcaaa tcccatatcc tgaccggttc gcggtaaagc
     2941 aggggtgcga aacgcgcgta ttccggacgc agcagccatt gttccatacc tttaatggtc
     3001 attttgaccg ttttgccgcc tcggtaaaga tagcggctga acaggacttc ttccccgaca
     3061 gcaattagga caatcttgcc gccgtccgca tcgatttgga agcggcagcg gttgatgccg
     3121 aagtccaaac tgccttccag caagagcacg aacgacacat aaggttcggc gaggcggctg
     3181 ctgcaaaaat cacaccgtgc cgttactgtg ccgccgtgga tggaaatgcc gttggacaaa
     3241 gtatcgaaac ggtagctgcc ctccacatag ctgccgccgc cgcttttgac tagattgacg
     3301 aaccggctgc tgttcaaatg ttctgctttt tccatagttt tctgataaag gttatcattt
     3361 gaaagataac atttttcgcc acagcaaaca atccgccgcc gacaacggca ttttcacaac
     3421 atttaccgtc cgtatgccgt ctgaaaattg cccgtcttcc cgataagcct taaaatacac
     3481 gccgtcaaac ctaccgtccg tccgtacatg agcccatccc cctttatcga aatgaaagac
     3541 gtcgccttcg cgtatggcga ccgcccgatt ctgaagaaca tcaatttcag cattccgcaa
     3601 ggcaattttg ctgccgtaat gggcggttcg ggcagcggca aaaccacgct gatgcgactg
     3661 attacaggac agattcgtcc gcagtccggg caggttttga ttgaaggacg ggatttggcg
     3721 ggcttttcgg ctgacgagtt gtatgaacac cgccgccgta tgggcgtatt gttccaacac
     3781 ggcgcgctgt ttactgattt gtcggtattc gacaatatcg cttttccgat gcgcgaactg
     3841 acgcgcctgc cggaagcggt gattcgggat ttggttttgt tgaaattgaa cgcggtcggt
     3901 ctgcgcggtg tggaaaacct gatgccgtcc gagttgtccg gcgggatgtc gcgccgcgtc
     3961 gcgcttgccc gcacgattgc gctcgaccct gaaattatgt tgtacgacga gccgtttacc
     4021 ggcctcgatc cgatttcctt gggcgtgatt gcccacttga tcagccgcgt caacaaggct
     4081 ttgcgttcga ccagtattat ggtaacgcac gacattgaaa aatctttgga aatcgtcgat
     4141 caggtgattt tcttggcgca cggcgaaatt atgttctccg gctcgccgca ggaaatgcgc
     4201 gaactggatt cgccttgggt gcgccagttt gtcggcgggc tggcagacgg ccccgtagca
     4261 taccgttatc cggcgcaaac gtcgttgcag caggacctgt tgggataaat gagttgatgc
     4321 cgtctgaaac cgttgaactt tacacataga aatttatgaa ctttatccgt tccgtcgggg
     4381 cgaaaaccct cggccttatt caatccctcg gcagtatcac gctgtttctg ctgaacattc
     4441 tggcgaagtc cggtacggct ttcgtccgtc cgcgcctgag cgtgcgccaa gtgtatttcg
     4501 ccggtgtgct gtcggtgttg attgttgccg tttcggggct gtttgtcggc atggtcttgg
     4561 gtttacaggg ctatacgcag ttgtcgaaat tcaaatccgc cgatattttg ggctatatgg
     4621 tcgcggcttc gctattgcgc gagctgggtc cggtattggc agcgattttg tttgccagca
     4681 gcgcgggcgg tgcgatgacc agcgaaatcg gtctgatgaa aacgaccgaa cagctcgaag
     4741 cgatgaatgt gatggcggta aaccctgtcg cccgcgtggt tgcgccgcgc ttttgggcgg
     4801 gcgtgttttc catgccgctt ttggcttcga ttttcaacgt ggcgggcatt ttcggtgcgt
     4861 atttggtcgg tgtaagctgg ctgggcttgg acagcggcat tttctggtcg caaatgcaga
     4921 acaacatcac gatacattac gatgtaatca acggtctgat caaatccgcc gcgttcggcg
     4981 tggcggtaac gctgattgcc gtgcatcagg gcttccactg tgtcccgact tcggaaggca
     5041 ttttgcgcgc cagcacgcgt acggtggttt cgtccgccct gacgattttg gcggtcgatt
     5101 ttatattgac cgcgtggatg tttacagatt gacagggcgt ctgaagacga aacgcggaac
     5161 atcggatatt caaggaactt taatgaaaaa gaacatattg gaattttggg tcggactgtt
     5221 cgtcctgatt ggcgcggcgg cggttgcctt tctcgctttc cgcgtggcgg gcggtgcggc
     5281 gttcggcggt tcggacaaaa cttacgccgt ttatgccgat ttcggcgaca tcggcggttt
     5341 gaaggtcaat gcccccgtca aatccgcagg cgtattggtc gggcgcgtcg gcgctatcgg
     5401 acttgacccg aaatcctatc aggcgagggt gcgcctcgat ttggacggca agtatcagtt
     5461 cagcagcgac gtttccgcgc aaatcctgac ttcgggactt ttgggcgagc agtacatcgg
     5521 gctgcagcag ggcggcgaca cggaaaacct tgctgccggc gacaccatct ccgtaaccag
     5581 ttctgcaatg gttctggaaa accttatcgg caaattcatg acgagttttg ccgagaaaaa
     5641 tgccgacggc ggcaatgcgg aaaaagccgc cgaataaacg tatgccatca tattcaaact
     5701 aggacactta atcatgaaaa aatcctccct catcagcgca ttgggcatcg gtattttgag
     5761 catcggcatg gcatttgccg cccctgccga cgcggtaagc caaatccgtc aaaacgccac
     5821 tcaagtattg agcatcttaa aaaacggcga tgccaacacc gcccgccaaa aagccgaagc
     5881 ctatgcgatt ccctatttcg atttccaacg tatgaccgca ttggcggtcg gcaacccttg
     5941 gcgcaccgcg tccgacgcgc aaaaacaagc gttggccaaa gaatttcaaa ccctgctgat
     6001 ccgcacctat tccggcacga tgctgaaatt aaaaaacgcc aacgtcaacg tcaaagacaa
     6061 tcccatcgtc aataaaggcg gcaaagaaat catcgtccgc gccgaagtcg gcgtacccgg
     6121 gcaaaaaccc gtcaacatgg acttcaccac ctaccaaagc ggcggtaaat accgtaccta
     6181 caacgtcgcc atcgaaggcg cgagcctggt taccgtgtac cgcaaccaat tcggcgaaat
     6241 tatcaaagcg aaaggcgtgg acggactgat tgccgagttg aaggctaaaa acggcagcaa
     6301 gtaatccata agccatgccg ccagactgaa gatggcatgg attttttcct accgaacact
     6361 atgcatacag aactcaacaa cggaacactg cacatcagcg gcgacatcac cgtcaaaacc
     6421 ctgaccgggg aggcttttga gcgtttccag cagcaatgcc gtctgaaaga aaccattgcc
     6481 gtcgatttcg gcggtgtcaa acgtgccgat tccgcctgcg tgtcgctgct gctcgaagtg
     6541 ctgcgcggtt gcaaaggcag cgtcaggctg accggcattc ccgaatccgt gcgcgcgctg
     6601 tccgaactgt acgaaatcaa agactggctg aaatcatgaa aaaaaccgcc tatgccttcc
     6661 tcctgctgat cgggttcgct tccgcccctg catttgccga aacccgcccc gccgaccctt
     6721 atgaaggcta caaccgcgcc gttttcaaat tcaacgacca agccgaccgc tacattttcg
     6781 cccctgccgc gcgcggctac cgcaaagttg cgccgaaacc cgtccgcgcc ggcgtgtcca
     6841 atttttttaa taacctgtgc gacgtggtca gcttcggcag caatatcttg cgcttggaca
     6901 tcaaacgcgc aagcgaagac cttgtccgcg tcggcatcaa caccactttc ggtttgggcg
     6961 ggcttatcga catcgccggc gcgggcggca ttcccgacaa taaaaacacc ttgggcgaca
     7021 cgtttgcttc gtggggatgg aaaaacagca attatttcgt gttgcccgtc ttagggccgt
     7081 ccaccgtccg cgacgcgctc ggcacgggta ttacctccgt ttattcgccc aagaatatcg
     7141 tcttccgcac ccctgtcgga cgctggggca cgactgccgt atccgccgtc agtacgcgcg
     7201 aaggcctgct cgatttgacc gacagcctgg acgaagccgc catcgacaaa tacagctaca
     7261 cgcgcgacct ctatatgaaa gtccgtgcgc ggcagaccgg tgcaacacct gccgaaggta
     7321 cggaagataa catcgacatc gacgaattgg tcgaaagtgc cgaaaccggc gcggcggaaa
     7381 ctgccgttca agaagattcc gtatccgaaa cacaggcaga agcagcaggg gaagccgaaa
     7441 cgcaacctgg aacacaacct taaagccatg ccgtctgaaa atccgtgttc ggacggcatt
     7501 cccttctgtc ggaacggaca gtccgaaacc acagattggg ctataatctc ttttttgcgc
     7561 ataccgcgcg acagcaaagg aaaaaccatg tacgaagtca accgcagcgt attcgtcctc
     7621 atccctttag aacctttttg gaactggctg caaaccctgc cgggcaacca cctcgacgga
     7681 ctgactcttg aagacatcca agccgacgcc aattcctacc ttgtccgccc gtgcgaaacc
     7741 gccgacgaag tatgggacga aatcgaagcc cgttttgaag acattttcgc cgccgaactt
     7801 gccgactggt gcgaagacga acgcgagtgg cctgcgctcg atgccgacat tttcaacgaa
     7861 tggttcgaca tccagctttc taccgtcatc accgacctcg aacacgaacc gcttgcccgc
     7921 gaagccttcc aacccatcaa cctgaactga tgaagctgac cgtccgcaac taccatctcg
     7981 acggctacgg ccatgtcaac aatgcgcgct acctcgaatt tttcgaagaa gcgcgctggg
     8041 cgttttttga agaacgcgga ctgctgcacg agttggcagg cctcatactg attgtcgccc
     8101 gcatcgacat ccgatacagc cgccccgccg tcgaaggcga cgtattgcag ttttcatgcc
     8161 gcctgaaaac ccccggcacg cgccgtattg tgctgaccca gacgattaca ctgccaaacg
     8221 gcaaaaccgc cgcagaagcc gacatcaccc tgatgcccgt ccacgccgcc acacaacgca
     8281 ccgtcagcct gcccgccacc cttgcccgcg cattggaagc cttgtccgaa tgaaaaaaat
     8341 actcaccgcc gccgtcgtcg cactgatcgg catcctcctt gccatcgtcc tcatccccga
     8401 cagcaaaacc gcgcccgcct tctccctgcc cgacctgcac ggaaaaaccg tttccaacgc
     8461 cgacctgcaa ggcaaagtaa ccctgattaa tttttggttt ccctcctgtc cgggttgtgt
     8521 gagcgaaatg cccaaaatca ttaaaacggc aaatgactat aaaaacaaaa acttccaagt
     8581 ccttgccgtc gcccagccca tcgatccgat agaaagcgtc cgccaatatg tcaaagacta
     8641 cggtttgccg tttaccgtca tgtatgatgc ggacaaagct gtcggacagg cgttcggcac
     8701 acaggtttat ccgacttccg tccttatcgg caaaaaaggc gaaatcctca aaacctacgt
     8761 cggcgaaccc gatttcggca aactctacca agaaatcgat accgcgctgg cacaatagcg
     8821 atgccgtctg aggcgcaaaa aaaaacagaa acgatgttcc tgttttttta gatcatccgc
     8881 cccgacacgt tcagacggca ttcaaccttc cggcagattc tttttcacca ctgccggatt
     8941 gcctgccgcc aaagagtagg gcggaatgtc ttttgtaacc accgcgcctg cgcccacgac
     9001 cgaaccgcgt ccgacggtta cgcccgccat tacaatcacc ctgcgccccg gccagacatc
     9061 gtcctccaac gtaatcggac ggatttccgt gtagccctca aagcgttttt tgaacggtca
     9121 aacttgtggt tatttgaata aaacagacat tccggctcca tcatcacatt tttgccgacc
     9181 accggcccac ggcagatttc acagtttgcc ccgatgcccg agccgtcgcc caaaaccgta
     9241 tccgggaaca catacgcccc gcgttcgata ttgaccccgc gtccgatatg cggagaaatc
     9301 cgccgtgcca gaaaaccgcg cacccgtctg ccgacgatgc cgatgccgcg catatgcgac
     9361 ggcggcaaca ccgaaccggc aaaacgcagc agcaggaaaa agaattttga tcccatcaaa
     9421 cctccctcaa aagaa
//
LOCUS       DAWKST010000073         9405 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.073, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000073 DAWKST010000000
VERSION     DAWKST010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9405
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.073"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            122..331
                     /locus_tag="WJL87_001210"
     CDS             122..331
                     /locus_tag="WJL87_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ7799636.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            420..1634
                     /locus_tag="WJL87_001211"
     CDS             420..1634
                     /locus_tag="WJL87_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ7799637.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKSYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVSQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIHDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(1721..4300)
                     /gene="clpB"
                     /locus_tag="WJL87_001212"
     CDS             complement(1721..4300)
                     /gene="clpB"
                     /locus_tag="WJL87_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ7799638.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHSGVNVPQVKQRLQQHLNSLPKVSGQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVENTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGIQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQDNIRNIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            4560..5570
                     /gene="trpS"
                     /locus_tag="WJL87_001213"
     CDS             4560..5570
                     /gene="trpS"
                     /locus_tag="WJL87_001213"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ7799639.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANSENNQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFRELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETVEFTKMLADGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            5576..5917
                     /locus_tag="WJL87_001214"
     CDS             5576..5917
                     /locus_tag="WJL87_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799640.1"
                     /translation="MPSEPLKGLPEQNVVRLTGKHPNDLEAVSGKCMETDDKGGPVGW
                     AENGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            6237..6602
                     /locus_tag="WJL87_001215"
     CDS             6237..6602
                     /locus_tag="WJL87_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799641.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAKEAVTEAKEAVTEAAKDTL
                     NKAADATQEAADKMKDAAK"
     gene            complement(6811..8319)
                     /gene="ppx"
                     /locus_tag="WJL87_001216"
     CDS             complement(6811..8319)
                     /gene="ppx"
                     /locus_tag="WJL87_001216"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEZ7799642.1"
                     /translation="MTTTPANILASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQSAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEVV"
     gene            complement(8436..9167)
                     /locus_tag="WJL87_001217"
     CDS             complement(8436..9167)
                     /locus_tag="WJL87_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ7799643.1"
                     /translation="MSREINAGGHHFLYGGISGGGVHYWDNKDFSEQCLHLSLGYKNR
                     SVTRSFGIVPFVGQNLLGGSRYNFVGGFNADFSQHLSERWRLTLNAGNMWKHYQEDRT
                     AARYDSHMPLAGATLMYSAPKDWLLYGGADWSHDLTKEAEQASVRKGLRIGAVKTFDG
                     GLGLRANLRYTRRTFDAPGTIVYRFPRKDHEYQANLSFWHDKISWKGFTPQLNFRYLK
                     IDSNMKSFYTRKNTQIFMSVEKDFK"
BASE COUNT         2187 a         2433 c         2467 g         2318 t
ORIGIN      
        1 gccttgtcct gatttttgtt aatccgctat atcgatttga atttttcaga aaatgaagcg
       61 gacgtttggg cggaggcaac ttgtttgata agatagcaat atttcaaaac ggagaaagat
      121 catgccttat gtcaatatta aagtaaccgg cggcaaggaa gcaccgactg ccgcgcaaaa
      181 agcggaactg atcggcggcg taaccgaatt gctggcacgc gtgctgggca aaaatcccga
      241 aacaacggtt gtcgtgattg atgaagtgga taccgataac tggggaatag gcggcaaaag
      301 cgtcagcgaa cggcgcaaag agggcaggta aaaagcctga aaatctcggt ttgatgcttt
      361 aaattccgcg tgaaaaagag tacattccca cccattgccc aaaatttacg gaacacatca
      421 tggataaatt tcccaagtct gcaaagctcg atcacgtctg ttacgacata cgcgggccgg
      481 ttcacaaaaa agcccttcag ttggaagagg agggcaataa aatccttaaa ctcaatatcg
      541 gcaaccctgc gccgttcggc tttgaagccc ctgatgaaat cttggtcgat gtcatccgca
      601 acctgccgac ttcgcaaggc tattgcgatt ctaaagggct gtattccgcc cgcaaagcca
      661 ttgttcacta ctatcagacc aaaggtttgc gcgatattac ggttgatgat gtctatatcg
      721 gcaacggcgt gtccgagctg attacgatgt ctatgcaggc attgctcaac gacggcgacg
      781 aaatcctgat tcccgcgccc gactaccctt tgtggacggc ggcggcaacg cttgcgggcg
      841 gtacggtacg ccattatctg tgcgacgaag aaaacggctg gttccccaac cttgccgata
      901 tggaagccaa aatcacgccc aaaaccaaag ccatcgtcgt catcaatccc aataatccga
      961 caggcgcggt gtacagcagg gaaatcctgt tggaaatcgc cgaactggcg cgcaagcacg
     1021 gtttgattat tttcgccgac gagatttacg ataaaatcct ttatgacggc gcggttcacc
     1081 accacatcgc cgcgcttgcc cccgaccttt tgacggtaac gttcaacggt ttgtccaaat
     1141 cctaccgcgt cgccggcttc cgccaaggct ggatggtgct caacgggccg aaacatcacg
     1201 caaaaggtta catcgagggt ttggatatgc tctcgtctat gcgcctgtgt gccaatacgc
     1261 cgatgcagca cgcgattcag acggcattgg gcggctatca gagcatcaac gaatttattt
     1321 tgcccggcgg gcggcttttg gaacagcgca acagggcgtg ggaactggtc agccaaatcc
     1381 ccggcgtgtc ctgcgtcaaa ccgatgggcg cgatgtatat gttcccaaaa atcgataccg
     1441 aaatgtaccg tatccacgat gacatgaaat tcgtttacga tttgctggtg cgcgaaaaag
     1501 tcttgctggt acagggaacg gggtttaatt ggattaaacc tgaccatttc cgcattgtca
     1561 cgctgcctta cgtccatcag attgaagagg cgatgggcag gttggcaaga ttcctgcaaa
     1621 cctaccgcca ataaggggac ggtttgtctg ccgaggataa aaaatgccgt ctgaaacgga
     1681 gattcccgtt tcagacggca ttttcaacag caggaacgaa tcaggcaaat ttcagtctgt
     1741 cgccgtcggc ttctaccctg atttcgcttt cgggcgcata gtttccggca agcagggctt
     1801 ttgccagcgg gttttcgatt tccgactgga tggcgcgttt gagcggacgt gcgccgtaaa
     1861 tcgggtcgaa accggctttg gcgatgatgt ccagagcggc atcggaaaca gtcaggcgga
     1921 ggttttgttt ttccaaacgt ttttccaagc ctttgagctg gattttcgca atgttgcgga
     1981 tattatcctg atccagtccg tggaacacga ccacttcgtc gatgcggttg atcatttcag
     2041 gacggaaatg ttctttcaca tcctccatca caacttcttt taccgcttcg taatcctgaa
     2101 tgcccatttg ttggatgtgc tggctgccga tgttggaagt catcacaatc acggtatttt
     2161 taaaatctac cgtcctgcct tgcccgtcgg tcaaacgacc gtcatccaat acttgcagca
     2221 ggatgttgaa cacgtccgga tgggcttttt ccacttcatc caaaagaatc acgctgtatg
     2281 gtttgcggcg gacttgctca gtcaggtagc cgccttcttc gtagccgaca tagcccggag
     2341 gcgcaccaat taggcgggca acagcatgtt tttccatgta ttcggacata tcgatacgaa
     2401 tcagatgatc ttcgctgtcg aacaggaagc ctgccaatgc cttgcacaat tcggtcttac
     2461 ccacgccggt cgggcccaag aacaggaagc tgccgtaggg tttattcggg tcggcaagac
     2521 cggaacggct gcggcggata gcgtcggaca cggcacgcac ggcttcgtct tgaccgacta
     2581 cgcggcggtg caacacttct tccattttca gcaacttgtc acgttcgcct tccatcattt
     2641 ttgacacagg aatgccggtc atacgggaaa caacttctgc gatttcctct gcgccgacat
     2701 tattacgcaa gagtttgttt gccggttttg tgctgtccgt atctgcccgt tcggcggctg
     2761 cacgctgttt ttccaaatgc tccaaatctt catacatcaa ttttgaagcc agtgccaaat
     2821 cgccttgccg ttttgcctgt tcgattttaa ttttgacttc gtcaatttgt ttcttaatat
     2881 tagcagcacc gtctgaaatt gctttttcgg ctttccagat ttcgtctaaa tcggcgtatt
     2941 ctttttgcag accgttgatt tcctcgtcta tcagttccaa acgttttttg ctggcatcgt
     3001 ctttttcttt ttcaacgtgc gccttttcca tccgaagctg aattagacgg cggtcgattt
     3061 tgtccattgc ttccggcttg gtttcttttt ccatcttgac acggctggcg gcttcgtcaa
     3121 tcaaatcaat cgctttatcg ggcaggaagc ggtcggtgat gtagcggtcg ctcaactccg
     3181 ctgcggcaac gatagcaggg tcggtaatat cgataccatg gtggatttca taacgctcct
     3241 gtaaaccgcg caaaatagcg atggtgtttt ccacgcttgg ctcgccgacc aatacttttt
     3301 ggaagcggcg ttcgagtgcc gcatcttttt cgatgtattg gcggtattcg tccaaagtgg
     3361 tcgcgccgat acagtgcaat tcgccacgtg ccaaagccgg tttcagcata ttgcccgcat
     3421 ccatcgcacc gtcggttttg cctgcgccga ccaaagtatg gatttcgtcg atgaaaatca
     3481 gagtgttgcc gtcgtctttc gccaaatcgt tcaacacgcc tttcaagcgt tcttcaaatt
     3541 cgccgcggta tttcgcgccg gcaatcaaag ccgccaagtc cagaaccaaa aggcgtttgt
     3601 tacgcaggga ttcaggcact tcgccgttga cgatacgttg cgccaagcct tcaacaatgg
     3661 cagttttacc cacgcccggt tcgccaatca gcacggggtt gtttttggtg cggcgttgca
     3721 gcacttgaat cgcgcggcgg atttcgtcgt cacggccgat aacggggtca agtttgccgt
     3781 cgcgggcgcg ctgggtcagg tcaagcgtat attttttcag cgcatcgcgt tggtcttcgg
     3841 cattggcatc gttcacgttt tgtcctcctc gtaccgcatc gattgcggca ttgatgtttt
     3901 gttcggtcgc gccggcttct ttcaaaattt tgccggccgc atcgttctgc tgcaccaagg
     3961 caagcaggaa aagttcactg gcgatatagg catcgccgcg tttggttgcg gctttgtcca
     4021 tcaggttcaa caccgcctgc aattcgcggc tgggcagaat gtcgccgccc tggccggaca
     4081 ctttcggcag gctgttcaaa tgctgctgca aacgctgttt cacctgcggc acgttcacgc
     4141 ccgaatgcgc cagcaacgcg gcggctccgc tgttttggtc gtcaagcagg gcttttaaca
     4201 caaagcctgc ttccagatag ctgccgtccg cagccaacgc caaactctga gcttctgcaa
     4261 gggcttgttg gaatttggcg gttaatttgt cgtatcgcat ttttgtttcc ttttcaaaat
     4321 gtccgctgtc gaagcctata tgtgcataat tgtggataac tcaagttctg ttttctgttt
     4381 ttctatattt aattcgatat atcattgaat ttaaagtata taaaaatgta taataatgtg
     4441 tataactata tcttcttaat atggaaaagt ctgttgtcgg ctggatgtag gtggcaaatc
     4501 gggtataatc ggcacatctt tttccctttc agacggcatt gatgccgcaa ggacatttta
     4561 tgagcaaaaa acgagttctg accggcgtaa ccaccaccgg catcccgcat ctgggcaact
     4621 acgtcggtgc tatccgcccc gccgtccgcg cggcgcaaaa ccctgatacc gaatccttcc
     4681 tcttcctcgc cgattaccac ggtatcatca aatgccacga gccggagatg attcaccaat
     4741 ccacccaagc cgttgccgcc acttggcttg cctgcggact cgaccccgag cgcaccacct
     4801 tctaccgcca aagcgacatt cccgaagtga tggaattgaa ctggattctg acctgtatca
     4861 ccgccaaggg tttgatgaac cgcgcccacg cctacaaagc cgccgtgcag gcaaattcag
     4921 aaaacaatca ggaagatccc gatttcggcg tggaaatggg tttgttcagc tatccgattc
     4981 tgatgaccgc cgacatcctt atgttcaacg ccaacgaagt gcccgtcggg cgcgaccaaa
     5041 tccaacacgt cgaaatggcg cgcgacatcg ccggtcgctt caatcaccgc ttccgagaac
     5101 tctttaccct gcccgaagtg aaaatcgacg aaaacgtcga actcttggtc ggtttggacg
     5161 gacgcaaaat gtccaaatcc tacggcaaca ccattccgct ttgggaaaac gacaaaaaaa
     5221 cccaaaaatc ggtcaacaaa atcatcacca atatgaaaga gccgggcgag ccgaaacagc
     5281 ccgacgaaag cccattgttt gaaatctaca aagccttctc cacgccgtct gaaacggtgg
     5341 aatttacgaa aatgcttgcc gacggcttgg cttggggcga ggcaaaaaaa cttttggcgg
     5401 cgaaaatcaa cgccgaactc gccgaaccgc gcgaacgcta caacgagctg accgccgacc
     5461 cttcgcaaat cgaagagatt ttgcaggcag gcgcgcaaaa agcgcgtaaa gaagcacgcg
     5521 aattgttgga caaagtacgc gatgcggtcg gcatccgccc gttgaaataa acccaatgcc
     5581 gtccgaacct cttaaaggct tgccggaaca aaacgtcgtc cggctgaccg gcaagcatcc
     5641 caacgacttg gaagccgtca gcggcaaatg catggaaacc gacgataagg gcggtccggt
     5701 aggctgggcg gaaaacggcg tgtgccatac cttgtttgcc aaactggtgg gcaatatcgc
     5761 cgaagacggc ggcaaactga cggattacct gatttcgcat tccgccctgc aaccctatca
     5821 ggcaggcaaa agcggctatg ccgccgtgca gaacggacgc tatgtgctgg aaatcgacag
     5881 cgagggggcg ttttatttcc gccgccgcca ttattgaggt attcggacat cccggaatat
     5941 atttgggttt ttcaaaccct gcaggaaaaa gtccgcaccg tcgggaaact caaaaggagg
     6001 gggatatgtg ttacaatttt ccgaactgtt ttcataaaat agttttcgga cgtgtttcaa
     6061 tatggcattg atgccgcctt attgttcgag aaaaaaacct ttatatttaa atataatggg
     6121 ttttaactaa acgggaaacc gttttctctc cggccgatgg gcaaaatcag ccgattgatg
     6181 gaacacggtc cgatttttaa gcaaaacctt aatcaacaac tcaagaagga taagacatga
     6241 aaaaattatt gattgccgca atgatggcgg ctgccttggc agcttgttcg caagaagcca
     6301 aacaggaggt taaggaagcg gttcaagccg ttgagtccga tgttaaagac actgcggctt
     6361 ctgccgccga gtctgccgct tctgccgtcg aagaagcgaa agaccaagtc aaagatgctg
     6421 cggctgatgc aaaggcaagt gccgaggaag ctgtaactga agccaaagaa gctgtaactg
     6481 aagccaaaga agctgtaact gaagccaaag aagctgtaac tgaagcagct aaagatactt
     6541 tgaacaaagc tgccgacgcg actcaggaag cggcagacaa aatgaaagat gccgccaaat
     6601 aatttgttgc cttggcaaaa atgatgggat gccgcctgcc ggcaacccaa acgaaaccgc
     6661 ctgaagattt tcaggcggtt tttgagttgt atgccccttg ttttttacgc ctgcatgacc
     6721 gttccgagca gatgtgccgc gcatatcggg atgttgcgga caggcaaatg ccgtctggac
     6781 agggtttgga cggcatttgt tccgtaaggt tcagacgacc tcgactttaa acggcatatt
     6841 gattttttgc cattggacgc tttcatattc caatgcgtcg gcaatcaggg gatggcgttc
     6901 cagccattcc ctgtcaatac gcaggatgaa gccgcagctt tccgtatccg tgcgcaactg
     6961 catatttttc gggaaagaca ggtcttggcg cgaacggcag aacagggcag caaggcgcag
     7021 ggacaaaacg gcataccaca acatttcgtt ggtgccgatg atgccgctca tttttttcat
     7081 atcgccgcga tgaccgatga ccagttgggc aagtatggtc tgttctttgc gtgagaaacc
     7141 cggcatatcg gcgttttcga ggatgtaggc ggaatgcttg tgatagccgg tgtgggcgat
     7201 gtccaaaccg atttcgtgca gcgcggcggc gcgtccgaga tactgttgcc acaaggcaag
     7261 ctcttgaact gtaacgtttt tagcgtggca gaggctgtcc ataaaggttt gcgcggtctc
     7321 ggcggtgcgt ttcgcctgat tgaggctgac gtggtagcgg tgttggaact cggcaaccgt
     7381 ttgtccgcgc atatcttcgt ttaaaccgcg cccgatcaaa tcgtaaaaca cgccgtcgcg
     7441 cagggcggct tcggttacgg tcatcctgtc gagtttcatt tcctcaaacg ccgccatcat
     7501 cacggcaagt ccgccggcaa aaacttcgat gcgttccggt ttcaggtttt caaatttggc
     7561 ttttttgacc gaaccggctt cgatgatgcg ttcggcgagg gcgcgcatgc ctttgtaggt
     7621 aatgtccgcc tcttggggca tttcggcggc aagcacgtcg cggatggatt ttgccgaacc
     7681 cgatgtgccg acggcgaaat cccaaccttc gcgcctcata tttttgctga tgcgctggat
     7741 ttcgttgcgg gcggcggaaa tggcagattg gaagtctttg gcggtgattt tgttttggaa
     7801 gaagcgcagg ctgtaggtta cgcagcccaa gggcaggctt tcagtaatgt cgggattcag
     7861 cgtcgagccg atgacaaatt ctgtcgaacc gccgccgatg tcgataacca gcattttgcc
     7921 gccgcccggg gggagggtgt ggatcacgcc ggtataaatc agccgcgcct cttcgcgccc
     7981 ggcgatgatt tcgatgggga aacccagtgc cgcttcggct ttgggaagga aatctgcgat
     8041 gtttttggcg acgcggaatg tattggttgc cacggcgcgt acctgttcag ggcggaagcc
     8101 gcgcaggcgt tcgccgaatt ttgccagaca gtccaaagcc tgttcttggg aagcggcact
     8161 caggtttttc tgttcgtcca gtccggcggc gaagcgcacc atctgtttga acgaatcgat
     8221 gacttttaat tgtccgttgt tgttttcgca aatctggagg cggaaactgt tggaacccaa
     8281 atcgacggag gcgaggatgt ttgcgggggt ggtggtcatg gcggatatcg gtgggggaaa
     8341 aacgcaatgt tactctgacg gcgcaggcgt tgacaataaa tgatgcggcg gtttttgatt
     8401 ctgtccacgg atgttgccga cggcattttt tgcgcttatt tgaaatcctt ttccacgctc
     8461 atgaaaatct gcgtgttttt gcgtgtgtaa aaacttttca tattgctgtc gattttcaga
     8521 tagcggaaat tgagttgcgg cgtaaagccc ttccaagaga ttttgtcgtg ccaaaacgac
     8581 aggtttgcct gatattcgtg gtctttgcgc gggaagcggt acacaatggt cccgggcgcg
     8641 tcaaacgtcc tgcgtgtata gcgcaggttt gcccgcagac ccaagccgcc gtcgaacgtt
     8701 ttgaccgcgc cgatacgcaa acccttgcgg acggaagcct gttccgcctc cttcgtcaag
     8761 tcgtgcgacc agtccgcccc gccgtaaagc agccagtctt tcggcgcgga atacatcagc
     8821 gtcgcgcccg ccagcggcat atggctgtcg tatcgggcgg cggtgcggtc ttcctgataa
     8881 tgcttccaca tattgcccgc gtttagtgtc aaccgccagc gttcgctcaa gtgttgggag
     8941 aaatcggcat taaagccgcc gacgaaattg tatcggctgc cgcctaagag gttttgcccg
     9001 acaaacggca cgatgccgaa cgagcgcgtt accgaacgat ttttatagcc gagcgacagg
     9061 tgcaggcact gttcgctgaa atctttgtta tcccaataat gcacgccgcc gccgctgatg
     9121 ccgccgtaga ggaaatgatg cccgcccgca ttgatttcgc gcgacacgcc taagccctag
     9181 cgcaaaccgt gtgccttttg cggcaggctg tcggcggttt tcgtccagct tctgcccgca
     9241 aattcaatcg tttttttgga cgaagcgttg tttatagcgg attaacaaaa atcaggacaa
     9301 ggcgacgaag cctcagacag tacagatagt acggaaccga ttcactcggt gcttcagcac
     9361 cttagagaat cgttctcttc gagctaaggc gagacaacgc cgtac
//
LOCUS       DAWKST010000074         9325 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.074, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000074 DAWKST010000000
VERSION     DAWKST010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9325
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.074"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            168..1511
                     /locus_tag="WJL87_001218"
     CDS             168..1511
                     /locus_tag="WJL87_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ7799644.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPAITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     DRYAPDALAKLSGKEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPVVVIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTGRVVALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRSKE
                     LLGWVAPQPDKYSITRTTLGHFLKNKLFKFNTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1514..2746
                     /locus_tag="WJL87_001219"
     CDS             1514..2746
                     /locus_tag="WJL87_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ7799645.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYTLAD
                     ALGINMSSEAGVLGKMLFGAIYFLPIYATVFAVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            2739..3515
                     /locus_tag="WJL87_001220"
     CDS             2739..3515
                     /locus_tag="WJL87_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ7799646.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVADAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            3515..4141
                     /locus_tag="WJL87_001221"
     CDS             3515..4141
                     /locus_tag="WJL87_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ7799647.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGIMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4145..4738
                     /gene="nqrE"
                     /locus_tag="WJL87_001222"
     CDS             4145..4738
                     /gene="nqrE"
                     /locus_tag="WJL87_001222"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ7799648.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            4752..5969
                     /gene="nqrF"
                     /locus_tag="WJL87_001223"
     CDS             4752..5969
                     /gene="nqrF"
                     /locus_tag="WJL87_001223"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ7799649.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPEEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            6121..7176
                     /locus_tag="WJL87_001224"
     CDS             6121..7176
                     /locus_tag="WJL87_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ7799650.1"
                     /translation="MPSETRLPNFIRVLIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKSGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     TGGYRTAMSSEFEKLLR"
     gene            7202..7417
                     /gene="nqrM"
                     /locus_tag="WJL87_001225"
     CDS             7202..7417
                     /gene="nqrM"
                     /locus_tag="WJL87_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ7799651.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRTIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(7541..8119)
                     /gene="grpE"
                     /locus_tag="WJL87_001226"
     CDS             complement(7541..8119)
                     /gene="grpE"
                     /locus_tag="WJL87_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ7799652.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            8303..9121
                     /gene="cysE"
                     /locus_tag="WJL87_001227"
     CDS             8303..9121
                     /gene="cysE"
                     /locus_tag="WJL87_001227"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ7799653.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
BASE COUNT         2253 a         2625 c         2313 g         2134 t
ORIGIN      
        1 taaaaacggc ttccgcaagg aaaaaagcag caaatctgac aaacaggtac aattcctgat
       61 agaatctaac actttgctac attaatttat tcaatgcttt tgatgccacg cgacacggta
      121 tcaaaacacg ccgtttttac cttaaccatc acaagtaatg caaatatatg attaaaatca
      181 aaaaaggtct aaacctgccc atcgcgggca gaccggagca agtcatttat gacggcccgg
      241 ccattaccga agtcgcgttg cttggcgaag aatatgccgg tatgcgcccc tcgatgaaag
      301 tcaaggaagg cgatgccgtc aaaaaaggcc aagtgctgtt tgaagacaaa aagaatccgg
      361 gcgtggtatt taccgcgccg gcttcaggca aaatcgccgc catccatcgc ggcgaaaagc
      421 gcgtacttca gtcggtcgtg attgccgttg aaggcaacga cgaaatcgaa ttcgaccgct
      481 acgcgcccga tgccttggca aaattgagcg gcaaagaagt gcgccgcaat ctgattcaat
      541 ccggtttgtg gactgcgctg cgtacccgtc cgttcagcaa aatccctgcc gtcgatgccg
      601 agccgttcgc catcttcgtc aatgcgatgg acaccaatcc gctggcggca gaccctgtgg
      661 ttgtgatcaa agaagccgcc gaggatttca gacgaggttt gctggtattg agccgtttga
      721 ccgagcgtaa aatccatgtg tgtaaagcag caggcgcaga cgtgccgtct gaaaacgccg
      781 ccaacatcga aacacatgaa ttcggcggcc cgcatccggc cggtttgagt ggcacgcaca
      841 ttcatttcat tgagccggtc ggtgcaaaca aaaccgtttg gaccatcaat tatcaagatg
      901 taattgccat cggacgtttg tttgcaacag gccgtctgaa caccgggcgc gtggttgcct
      961 tgggtggttc tcaagtcaac aaaccacgcc tcttgcgtac cgttttgggt gcgaaagtat
     1021 cgcaaattac tgcgggcgaa ttggttgacg cagacaaccg cgtgatttcc ggttcggtat
     1081 tgaacggcgc gattgcacaa ggcgcgcacg attatttggg acgctaccac aatcagattt
     1141 ccgttatcga agaaggccgc agcaaagagc tgctcggctg ggttgcgccg cagccggaca
     1201 aatactccat cacgcgtaca accctcggcc atttcctgaa aaacaaactc ttcaagttca
     1261 acacagccgt caacggcggc gaccgcgcca tggtgccgat tggtacttac gagcgcgtga
     1321 tgcccttgga tatcctgccc accctgcttt tgcgcgattt aatcgtcggc gataccgaca
     1381 gcgcgcaggc attgggttgc ttggaattgg acgaagaaga cctcgctttg tgcagcttcg
     1441 tctgcccggg caaatacgaa tacggcccgc tgttgcgcaa agtgctggaa accattgaga
     1501 aggaaggctg attatgggct tgaaacattt tctggaaaaa atcgaaccgc acttcctgcc
     1561 gggcggcaaa catgaaaaat ggtatgccct ctacgaagcg gcggcgacga ttttctacac
     1621 atccggtgcg gtaacgcgca aagcggcaca cgtccgcgac gcgctcgact ccaagcgcat
     1681 gatgattttg gtgtggctgg ctttgttccc cgccatgttt tacggcatgt acaacgtcgg
     1741 cgcacaggca ttcggtgcct taacgcccga tttgctgcaa caaagcatcg ccaacgactg
     1801 gcattacacc cttgccgacg ctttgggcat caatatgtcg tctgaagcgg gcgtgttggg
     1861 caaaatgctg ttcggcgcga tttacttcct gccgatttac gcgaccgtat ttgccgtcgg
     1921 cggtttctgg gaagtcttgt tcgccaccgt acgcaaacac gaaatcaacg aaggtttctt
     1981 tgttacctcg attctgtttg ccttaatcgt tccgcccacg ctgccgctgt ggcaggcggc
     2041 tttgggtatt tctttcggcg ttgtggttgc gaaagaagta ttcggcggta cgggtaaaaa
     2101 ctttatgaac cctgcgctgg caggccgcgc cttcctgttc ttcgcctacc ccgccaactt
     2161 gagcggcgat gcggtttgga cggcggttga cggctattcc ggcgcaaccg cgctggcgca
     2221 atgggcggca cacggtgcag acggcctgaa aaacgccgta accggtcaaa ccatcacttg
     2281 gatggacgcg tttatcggca aactgcccgg ctccatcggc gaagtctcca ctttggcact
     2341 cttaatcggc ggcgcgttta tcgtgtttgc ccgcatcgct tcttggcgca ttattgccgg
     2401 cgtgatgatc ggtatgattg cgatgtcttc gctgttcaac ttcatcggtt cggacaccaa
     2461 cgctatgttt gccatgcctt ggtactggca tttggtcgtc ggcggcttcg ccatcggtat
     2521 gctgtttatg gcgaccgacc ctgtttccgc ttcctttacc aatgtcggca aatggtggta
     2581 cggcgcgctg atcggcgtga tgtgcgtgtt gatccgcgtg gtcaatccgg cttaccccga
     2641 aggtatgatg ttggcgattc tgtttgccaa cctgtttgcc ccgattttcg actatttcgt
     2701 cgcacaagcg aacatcaaac gcagaaaggc gcgcagcaat ggctaagaaa ttcgataaag
     2761 acagcttcag cggcacgctg attgtcgtgt tggcggtcag cctgatttgc tcggtcatcg
     2821 ttgccggtgc ggtcgtcggc ttgaaaccca tccaagagaa acaaaaactc caagacaaac
     2881 aaggctatat cttgagcgta gccggtttga tggataagga caccgacatc ggcaaaacct
     2941 ttgccgagcg tatcgagcaa cgtgttgtcg atttggcgac cggcgaatac gtcgccgatg
     3001 cgcctaaaga cttcagcgcg cgcatcgcag gcaaagaccc tgcccaaagc atccgcatca
     3061 aacccgaaga cgatttggca ggcatcaaaa gccgtgccaa atacaccgag gtttatttgg
     3121 taaaaggcga agacggcaaa atcgggcaaa tcatcctgcc tatgcacggt aacggtttgt
     3181 ggtcggtcat gtacggcttt gtcgccatcc agcccgacgg caacaccatc aacggcatta
     3241 cctactacga acaaggcgaa accccgggct tgggcggcga aatcggcaat ccgttgtggc
     3301 agcaaaaatt cgtcggcaaa aaactgtttg acggacaagg caaactcgcc ctgcacgtcg
     3361 gcaaaggcgc gggttcggac aaagaacacg gcgtagatgc cctctccggc gcatcgctga
     3421 catccaaagg cgtgcaaggt tcgttcgcct actggttcgg cgaaaacggc tatatcccct
     3481 acctgaacaa attgaaatca gcaggagcgc aataatggct gatatgaaac gcttgaaaca
     3541 tttgatgttt tcacccttta tcgacaacaa cccgattgcc ttgcaggttt tgggtatttg
     3601 ttcggctttg gcggttacca ccaaacttca gacggccatc gtgatgggta tttccgtagc
     3661 tttggtaacc ggtttttcca gcttcttcat ttcgctggta cgcaactaca tccccaacag
     3721 catccgtatt atcgtgcaaa tggcgattat cgcgtcgctg gttacgctgg tcgaccaact
     3781 cttgcaggca tttgcctacg aattgtccaa acagctttcc gtattcgtcg gtctgattat
     3841 taccaactgt atcgtgatgg gccgcgccga agcatttgcg atgaaagagc cgccgctgga
     3901 aagcctgatc gacggcatcg gcaacggcgc gggctacggg ataatgctgc ttgtcgtcgc
     3961 caccgtccgc gaactgattg gctcgggcaa actcttgggc tacaccgttt tccaaaccgt
     4021 gcaggacggc ggctggtatc agaccaacgg cttgttcctg ctcgccccca gcgcgttctt
     4081 catcatcggc tttttgattt ggggactgcg tacctggaaa cccgaacagg cggaggaata
     4141 agctatggaa cactatttga gcctcttcat caaatccgtc ttcattgaaa atatggcact
     4201 atccttcttt ttgggtatgt gcacgttttt ggcggtatcc aaaaaagtat ccaccgcatt
     4261 cggtttgggt gtggcggtaa ttttcgtact cgggctgtcc gtccctgtca accaactcgt
     4321 ttactcgctg ctcaaagacg gcgcgattgt cgaaggcgtg gatttgacct ttttgaaatt
     4381 catcaccttc atcggcgtga ttgccgcttt ggtgcagatt ttggaaatgt tcttggataa
     4441 attcgtcccc gccctctata acgcattggg catctacctg ccgctgatta ccgtgaactg
     4501 cgcgattttc ggcgccgtat cgtttatggc gcaacgcgaa tacaacttcg gcgagtccgt
     4561 tgtatacggc ttcggcgcgg gcttgggctg gatgttggcg attgtcgctt tggcgggcat
     4621 taccgaaaaa atgaaatatt cggacgctcc caaaggcctc aaaggcttgg gcattacctt
     4681 tatcgccgcc ggcctgatgg cgatggcgtt tatgtcgttc tctggcatcc agttataaga
     4741 aagggatcgg tatggagatt attttaggta tcgtgatgtt taccgtcatc gttttggttt
     4801 tggcactgat gattctgttt gccaaatcca aactggtcag cgaaggcgac atcaccatca
     4861 aagtcaacgg cgaaaaagag ctgacgatgc ccgccggcgg caaactcttg ggcgcgcttg
     4921 ccaacgaagg catctttatc ccctccgcct gcggcggggg cggttcgtgc ggacaatgcc
     4981 gcgtcgtcgt aaaaagcggc ggcggcgaca tcctgccgac cgagctgtcc cacatcagca
     5041 aacgcgaagc acgcgaaggc tgccgtctgt cgtgccaagt caacgtcaaa accgacatgg
     5101 acatcgaagt ccccgaagaa gtgttcggcg tgaaaaaatg ggaatgcacc gtcatctcca
     5161 acgataacaa agccacgttc attaaagaac tcaagcttgc cattcccgaa ggcgaagaag
     5221 tccccttccg cgccggcggc tacattcaaa ttgaagcccc gccgcacacc gttgcctaca
     5281 aagacttcga cattcccgag gaataccacg aagactggga caaatacaat ttgtggcaat
     5341 acgtttccaa agtggacgag ccgattttgc gtgcctactc tatggcttcg tatcctgaag
     5401 aaaaaggcat cattatgctg aacgtgcgta tcgccacgcc gcccccgcgc gtacctgatg
     5461 cgcctccggg acaaatgtcg tcctacatct ggtcgctcaa acccggcgac aaagttacga
     5521 tttccggccc gttcggcgaa tttttcgcca aagacaccga tgccgaaatg gtatttatcg
     5581 gcggcggtgc gggtatggct ccgatgcgtt cccacatttt cgaccagttg aaacgtttga
     5641 actccaaacg taagattacc ttctggtacg gcgcacgttc caaacgcgaa atgttctatg
     5701 tcgaagactt cgaccaactc gcagcagaat tcccgaactt cacatggcac gtcgccctgt
     5761 ccgacccatt gcctgaagac aactgggacg gctacacagg cttcattcac aacgtggttt
     5821 acgaaaacca tctgaaaaac cacgaagcgc cggaagactg cgaattttat atgtgtggtc
     5881 ctccgatcat gaaccagtcc gtcatcaaaa tgcttaaaga cttaggcgtg gaagacgaaa
     5941 acatcctctt ggacgatttc ggcggttaaa tattcggctt caaacacaaa aggaagaaac
     6001 ctcggtttct tcctttttct atccgatgcc gcccgaaccc ccttcagacg gcatcggcat
     6061 agaaacatat cggccgtttt gcgatataat tcaaaccatt tgccaaaacc gtcaaacact
     6121 atgccgtctg aaacacgcct gccgaacttt atccgcgtct tgatatttgc cctgggtttc
     6181 atcttcctga acgcctgttc ggaacaaacc gcgcaaaccg ttaccctgca aggcgaaacg
     6241 atgggcacga cctataccgt caaatacctt tcaaataatc gggacaaact cccctcacct
     6301 gccgaaatac aaaagcgcat cgatgacgcg cttaaagaag tcaaccggca gatgtccacc
     6361 tatcagcccg actccgaaat cagccggttc aaccaacaca cagccggcaa gcccctccgc
     6421 atttcaagcg acttcgcaca cgttactgcc gaagccgtcc gcctgaaccg cctgacacac
     6481 ggcgcgctgg acgtaaccgt cggccccttg gtcaaccttt ggggattcgg ccccgacaaa
     6541 tccgttaccc gtgaaccgtc gccggaacaa atcaaacaag cagcatctta tacgggcata
     6601 gacaaaatca ttttgaaaca aggcaaagat tacgcttcct tgagcaaaac ccaccccaag
     6661 gcctatttgg atttatcttc gattgccaaa ggcttcggcg ttgataaagt tgcgggcgaa
     6721 ctggaaaaat acggcattca aaattatctg gtcgaaatcg gcggcgagtt gcacggcaaa
     6781 ggcaaaaacg cgcgcggcga accttggcgc atcggcatcg aacagcccaa catcgtccaa
     6841 ggcggcaata cgcagattat cgtcccgctg aacaaccgtt cgcttgccac ttccggcgat
     6901 taccgtattt tccacgtcga taaaagcggc aaacgcctct cccatatcat taatccgaac
     6961 aacaaacgac ccatcagcca caacctcgcc tccatcagcg tggtcgcaga cagtgcgatg
     7021 acggcggacg gcttgtccac aggattattc gtattgggcg aaaccgaagc cttaaagctg
     7081 gcagagcgcg aaaaactcgc tgttttcctg attgtcaggg atacaggcgg ctaccgcacc
     7141 gccatgtctt ccgaatttga aaaactgctc cgctaaacat acaaaccgat taggaaacac
     7201 catgaaaacc ctgctcctca ccttcggcat cttcctgacc gtcatcatcg gtatggcggt
     7261 cggctatatc ttctccaaac gcaccatcaa aggcagttgc ggcggcatta ccgccttggg
     7321 tatgaaaaaa atgtgcgact gcgacacgcc ttgcgacacc ctgcaaaaga aactggatga
     7381 agaaaaacag gcaggcggca tacgggttga ccgttaacac cgaaacaaaa tgccgtctga
     7441 agcccgacag gcgggttcag acggcattgc cgctttactt cgatcggttt atacgcaaaa
     7501 cgcgcttcag gaaataaatc agattattcc ccagacgcct tcaggcttcc ttctgtgcca
     7561 ccgtaaccat agccgggcgc aacacgcggt cggacagcgt ataacccttc ttcatcacgc
     7621 ccaccacggt attgggttcc tgttcgcttg ccaccgcctg catcgcctga tggatattcg
     7681 gatcgagctt atcgcccgct ttaggattga tttccttgat ttgcgtagca tcaaatgctt
     7741 tctgcaactc gttcaaagtc atctgcacgc ccattttcag cgcatcgaaa ttgccgctct
     7801 gatccaaaag cgccatttcc agataatcct tgaccggcag catttccacg gcaaacttct
     7861 gtccggcgaa cttgtgcgta tccgcaattt cctgctggtg gcggcggcgc aggttttgct
     7921 cgtttgccaa agcgcgcagc tgctcgtctt tcaactgcgc ttccagctcg gcaatccgcg
     7981 cctgcaaatc ctcataagcc ggctctgcgg cagcctgttc ctgcacaccg tccgcatttc
     8041 ctactgtctc gacggtttcc accgcctcca cattttcaac cgcttcttca ctgttttgct
     8101 gctgtgtctg ttcgctcata tcgtatccct taaaacaaat tggaaatcaa aatccagtta
     8161 ttacccgcgc aagataagga cattttcaag aaacttcaag caaaagcaga gaatttcaaa
     8221 ttcattttca aatcccctac cgaaaaaata atatagacgg taagatactg ccgaccatat
     8281 cgattttcaa acgtcatcac tcatgaaaaa agaccattta aacacaaccg gtttcgacct
     8341 ctggcacacc atccgcgaag aaaccgcggc cgcggtctcc gccgaaccga tgctggcaag
     8401 ttttttgcac caaaccgtgt tgcgccacga gtccctcggc tccgtccttg cctaccacct
     8461 ttccagcaaa ctcggcagcc cgattatgga cgtgcgcgcg ctgtttgaaa tctaccagca
     8521 ggcgttgggc agcgacaccc aaatcggcaa atgcgtcgaa gcagacttaa aagccatcta
     8581 cgaacgcgat cccgcctgcg acgaatattc gctgccgctt ttatatttca aaggcttcca
     8641 cgcgattcag gcacaccgca tcaaccatcg gctgtatctc gacgggcgca aaacgctggc
     8701 gtatttcctg caaaaccgta tgtccgaagt attcggcgtg gacatccatc ccgccgcccg
     8761 tttcggatac gggctgatgc tcgaccacgc caccggcttt gttgccggcg aaaccgccgt
     8821 gttgggcaac aatatttcga ttttgcacgg cgtaacgctc ggcggttcgg gcaaagaagg
     8881 cggcgaccgc caccctaaaa tcggcgacgg cgtgatgatc ggcgcaaacg cctcgatatt
     8941 gggcaatatc cgcatcggca gcaatgccaa aatcggggcg ggcagcgtcg tggtttcaga
     9001 cgtgccgccg tccatcacgg ttgtcggcgt acccgccaaa cccgtggcgc gatcgctcaa
     9061 aacaccgtcg gcggatatgg atcaaaatat ccagtttacc gaaatcgact ttatgatttg
     9121 aatatccgca ccaatgccgt ctgaaacgcc aaacgggctt cagacgacat tgcccgcttc
     9181 aagcctgcgc cttgatatgc cgccgatgtc ggcacttgcc agtcggacaa aatgcctttt
     9241 attcaataca cccgattccc acgcaaccgg aaatatagtg gattaacaaa aatcaggaca
     9301 aggcgacgaa gccgcagaca gtacg
//
LOCUS       DAWKST010000075         9187 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.075, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000075 DAWKST010000000
VERSION     DAWKST010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9187
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.075"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..299)
                     /locus_tag="WJL87_001228"
     CDS             complement(<1..299)
                     /locus_tag="WJL87_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ7799654.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYIDEVLTAGHGKLYQSDGRHR"
     gene            538..1437
                     /gene="crgA"
                     /locus_tag="WJL87_001229"
     CDS             538..1437
                     /gene="crgA"
                     /locus_tag="WJL87_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ7799655.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1664..4879)
                     /gene="carB"
                     /locus_tag="WJL87_001230"
     CDS             complement(1664..4879)
                     /gene="carB"
                     /locus_tag="WJL87_001230"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ7799656.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFKLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQVLGYGICATRGTA
                     QYLTEQGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(4886..5434)
                     /locus_tag="WJL87_001231"
     CDS             complement(4886..5434)
                     /locus_tag="WJL87_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799657.1"
                     /translation="MRTIFFSGSRSISRLNPQIRERINNILSNNFDIVIGDANGADKA
                     IQKFLQEQDYANVHIYFSGKIYRNNVGNWQFVQVDSKGTGRVFYTAKDKKMAEIADYG
                     FILWDGKSIGSLNNIAELLQLNKPSLVYHSQTKEFFKIKSSADLENILSNIEDDVLAS
                     ILEKGNTFLKSYVTKQPSLIQE"
     gene            complement(5437..6000)
                     /locus_tag="WJL87_001232"
     CDS             complement(5437..6000)
                     /locus_tag="WJL87_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ7799658.1"
                     /translation="MAIELNGNWTKGFALDHHMQKSILLGYDELGHPHFDSKRSVIGE
                     WVYQLKYRNQTQNVSLLVDYILQKFSGLESINLIVPAPFTTERINQPVQLIAKELSDR
                     LNIPYSPILRKSSSHTPLKNIEEKSEKLEILNNSITIDNIDLSNKNILVIDDLFDSGA
                     TLEISTEKLFSKNARSVIVLAMTKTRG"
     gene            complement(6407..6574)
                     /locus_tag="WJL87_001233"
     CDS             complement(6407..6574)
                     /locus_tag="WJL87_001233"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799659.1"
                     /translation="MADFDWIIENNRIKPTELSIYKGRLKNKKQPAQPVFPAEPLIPT
                     AAPSSLPVGKR"
     gene            complement(6578..6967)
                     /locus_tag="WJL87_001234"
     CDS             complement(6578..6967)
                     /locus_tag="WJL87_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ7799660.1"
                     /translation="MSTHEKLLTADNPVLHQRAKAMRQEMSEAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(7559..7684)
                     /locus_tag="WJL87_001235"
                     /pseudo
     CDS             complement(7559..7684)
                     /locus_tag="WJL87_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(7730..8863)
                     /gene="carA"
                     /locus_tag="WJL87_001236"
     CDS             complement(7730..8863)
                     /gene="carA"
                     /locus_tag="WJL87_001236"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ7799661.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         2088 a         2302 c         2351 g         2446 t
ORIGIN      
        1 cggtgtctgc cgtcgctttg gtagagtttg ccgtgtccag cggttaatac ttcgtctatg
       61 tattttttca ctgtccaagg ctcgtgcatc caccagcccg gcatctgcca aatcacggca
      121 tccatccaaa cgaatttttc gatttctgcc tcaacatcat agccggcatc aatcacggtt
      181 tcttgaacat tgtgtccgag cgcggtcaaa acttctttcg cttttttgtg aagcgtgcgg
      241 tttaacccgc cgtgagaatg tccgaacgcc ttgccgccgt ctaataataa aatattcatt
      301 tgttacctcg tttgtgaatt gatgaatgta ttgtgccgtt ttactttata atttaaaagt
      361 gcaaaaataa gaaaacactt ttgcgtcaaa tgaaataatc agatgtttca acacacggga
      421 tgacacataa agcgtctccc tatgtgccgt cctgattcgg aaggggttac acccctccca
      481 aataaagtct gatcctgccg ccctaaaggg cggggttcca atcgaaaagg aaacacgatg
      541 aaaaccaatt cagaagaact gaccgtattt gttcaagtgg tggaaagcgg cagcttcagc
      601 cgtgcggcgg agcagttggc gatggcaaat tctgccgtaa gccgcatcgt caaacggctg
      661 gaggaaaagt tgggtgtgaa cctgctcaac cgcaccacgc ggcaactcag tctgacggaa
      721 gaaggcgcgc aatatttccg ccgcgcgcag agaatcctgc aagaaatggc agcggcggaa
      781 accgaaatgc tggcagtgca cgaaataccg caaggcgtgt tgcgcgtgga ttccgcgatg
      841 ccgatggtgc tgcatctgct ggcgccgctg gcaacaaaat tcaacgaacg ctatccgcat
      901 atccgacttt cgctcgtttc ttccgaaggc tatatcaatc tgattgaacg caaagtcgat
      961 attgccttac gggccggaga attggacgat tccgggctgc gtgcacgcca tctgtttgac
     1021 agccgcttcc gcgtaatcgc cagtcctgaa tacctggcaa aacacggcac gccgcaatct
     1081 acagaagagc ttgccggtca ccaatgttta ggcttcaccg aacccggttc tctaaataca
     1141 tgggcggttt tagatgcgca gggaaatccc tataagattt caccgcactt taccgccagc
     1201 agcggtgaaa tcttacgctc gttgtgcctt tcaggttgcg gtattgcttg cttatcagat
     1261 tttttggttg acaacgacat cgctgaagga aagttaattc ccctgctcgc cgaacaaacc
     1321 tccgataaaa cacacccctt taatgctgtt tattacagcg ataaagccgt caacctccgc
     1381 ttgcgcgtat ttttggattt tttagtggag gaactgggaa acaatctctg tggataattc
     1441 tataaaaacc tgccataaaa ttgtttttac tcacttaata actttgagaa aaaataaata
     1501 agatgatgta tagccggcgg caatggcagt ctatatgggg agcgtgatag ggctgttcct
     1561 tatgtacggc ttgtgtgtgg caacatcggc tttccaaatg acaaaatgcc gtctgacacc
     1621 gtaaaaaaag gtttcagacg gcattttcaa cctgattcag gcatcagttg cggtttttca
     1681 aacgcccgtg caactcttga acgctgtaca cgcccagatg gtctcggctt ttcgcacctt
     1741 cgctcatcgc ttcgccgccg gcagtggtgg tgtattgcgg cacacgttgc tgcaatgcgc
     1801 tttggcggat gatgtggctg tcggacacgg attgcggatc gctggaaacg gtattcacga
     1861 ccagtgcgat ttcaccgttt ttcagtgcgt cgccgatgtg cgggcggcct tcggggactt
     1921 tattgatggt ttgtacaatc agtccctgtt cggtcaggta ttgcgccgtg ccgcgtgtgg
     1981 cgcagatgcc gtagcctaaa acctggaagt ttttagcggt tttgataacg cgttctttgt
     2041 cttcttcgcg cacggagagg aagattttgc cggtcgggtt gaggcgttcg cccgcaccga
     2101 gttgggcttt gtagtaggct tcgccgaagc ttgcgcccac gcccatcact tcgccggtgg
     2161 agcgcatttc cggtcccaaa atcgtgtcta cgcccgggaa tttgatgaat gggaacacgg
     2221 cttctttaac ggcatagaaa tcggggacaa cttctttttc cacgccttgt tctttcaggg
     2281 aaatacctgc catgcagcgt gcgccgactt tggcgagcgg cacgccggtg gctttggaga
     2341 cgaagggcac ggtacggctg gcgcgcgggt tcacttccaa cacgaacacc acgccgtcct
     2401 gcacggcaaa ctgcacgttc atcagaccga ccacgcccag cgcgtacgcc atcgctttgg
     2461 tttggcggcg gatttcgtct tggatttctt cgcttaagga gtagggcggc agcgagcagc
     2521 cggagtcgcc ggagtggatg cccgcctgtt cgacgtgctg cataatgccg ccgataacca
     2581 cgtctttgcc gtctgaaacg cagtccacat cgacttcaat cgcgttgttg aggaagaaat
     2641 cgagcaacac ggggctgtct tcggaaacct gcacggcttc gcgcatgtat ttttgcaagg
     2701 cttcggcaga gtggacaatc tgcatggcgc ggccgcccaa aacataagac gggcgcacga
     2761 ccagcggata gccgatttct tcggctttga cgagtgcttc ttcttcgttg tgggcgatgc
     2821 ggttgggcgg ttggcgcaga cctaagtcgt tcaacacttt ttggaagcgt tcgcggtctt
     2881 cggcggcgtc gatgctgtcg gcggatgtgc cgatgatgtt tacgccgttt tcaaccagcg
     2941 cgttggcgag tttgagcggg gtttgaccgc cgtaatgcac aatcacgccc cacgggtttt
     3001 cggtgcggac gatttccaac acgtcttcca atgtcagcgg ctcgaaatag aggcggtcgc
     3061 tggtgtcgaa gtcggtggac acggtttctg ggttgcagtt caccataatc gtttcaaagc
     3121 ccgattcgcg cagggcgagt gcggcgtgaa cgcagcagta gtcaaactcg atgccctgac
     3181 cgatgcggtt cgggccgccg ccgagaatca tcactttttt acggtcggaa ggacgggatt
     3241 cgcattcttc ttcgtaagtg gagtaaagat aggcggtttc ggtggcgaac tcggcggcgc
     3301 aggtgtcaac gcgtttgtaa accggatgca gcttcagcgc gtagcggtgt tcgcgcactt
     3361 ctttttcgct tacgttcaac aattgtgcca aacgtttgtc ggagaagcct ttgcgtttca
     3421 gacggcgtag ggcggcgaaa tccaaatcac tcaaaatgcc gtctgaaacc gctttttctt
     3481 ccttcatcaa gtcttcgatt tgcgccagga accaagggtc gatggcgcag atttcgtgga
     3541 tttcttccag cgtgaagccg gcgcggaacg cgtctgccac aaacagcata cgttcggggc
     3601 cggggtttgc cagttcgcgg cggatttccg ctttgtcgga gcttctcgga ttgaagccgc
     3661 acaagcctgt ttccaagccg cgcagggctt tttggaagct ttcctgaatc gtgcggccca
     3721 tcgccatcac ttcgcccacg gatttcatct gcgtggtcag gcggtcgtct gcggcaggga
     3781 atttttcaaa cgcaaaacgc gggattttgg ttaccacata gtcgatggac ggctcgaatg
     3841 acgcgggggt tttgccgccg gtgatgtcgt tgcgcaactc gtccagctta aagccgaccg
     3901 ccagtttcgc cgccaccttc gcaatcggga agcccgtcgc tttggaagcc agcgcggacg
     3961 aacggctcac gcgcgggttc atctcaatca caatcatctc gccgttttca gggttcaccg
     4021 caaactgcac gttcgagccg ccggtatcca cgccgatttc gcgcaatact gccaacgaag
     4081 cattacgcat gatttggtat tctttgtccg tcagcgtttg cgcaggcgca accgtaatcg
     4141 agtcgcccgt atgcacgccc atcgggtcga agttttcaat cgagcagata atgatgcagt
     4201 tgtcgttctt gtcgcgcacc acttccatct cgtactcttt ccagccgaga acagactgct
     4261 caatcagcag ctcgtgcgta ggcgacgcat cgaaaccgcg ttcgcaaatc gccaaaaact
     4321 catctttatt gtaggcaatg ccgccgcccg aaccgcccat cgtgaaagac ggacgaatca
     4381 gggtagggaa gccgacctgt tcttgcgctg ccaaagcttc gttcatcgtg tggcagacaa
     4441 aagatttcgg gcaggagagg ccgatttttt ccatcgcttc tttaaaacga ccgcggtctt
     4501 ccgccttgtc aatcgcgtct tccgtcgcgc cgatcagctc gacattgtat ttcgccagca
     4561 cgccgttgcg cgccaaatcc agcgcacagt tcagcgcggt ctgaccgccc atcgtgggca
     4621 gaatcgcatc aggacgctcc ttggcgataa tcttctccac cgtctgccac ataattggct
     4681 cgatgtaggt aacatccgcc atttcagggt cggtcatgat ggtggcgggg ttggaattca
     4741 ccagaatgac tttatagcct tcttcacgca aagccttaca ggcctgtgcg cctgaatagt
     4801 caaattcgca ggcctgaccg ataacgatag ggccggcgcc gatgataagg atggatttta
     4861 ggtcggtacg tttgggcatg ggtggttact cttgaattaa agaaggttgt tttgttacat
     4921 agcttttaag aaaagtattt cccttttcta aaattgaagc taaaacatca tcttctatgt
     4981 tagataaaat attctctaaa tctgcgcttg atttaatttt aaagaattct tttgtttgag
     5041 aatggtatac aagagaaggt ttatttagct gtaaaagttc tgctatatta tttaatgaac
     5101 caatactctt accatcccat aaaataaagc cataatcagc aatttctgcc attttcttat
     5161 cttttgctgt ataaaatacc cttccagttc ctttgctgtc tacttggaca aactgccaat
     5221 tccccacatt atttcggtaa attttgccag aaaagtaaat atgcacatta gcataatctt
     5281 gttcttgtag aaatttttgg atagctttat cagccccatt agcatctcca ataacaatat
     5341 caaaattatt gctaagaata ttgttaattc tttctcgtat ttgtggattt agtcgtgaaa
     5401 tgctgcgtga accagaaaag aaaatagtgc gcatttttat cctctagtct ttgtcattgc
     5461 taaaacaatt acagatctag cattttttga aaacaatttt tcagttgata tttccaatgt
     5521 tgctccgcta tcaaataaat catcaattac taaaatattc ttattggata gatctatatt
     5581 gtcaattgtg atggaattat tcagtatttc tagtttttct gatttttctt caatattttt
     5641 gagtggtgtg tgtgagctag attttcttaa aataggagaa tatggaatat tcaagcgatc
     5701 acttagttct ttggcaatta attgaactgg ttgattaata cgctctgttg taaaaggggc
     5761 gggaacaatt aaatttatag attctaaacc actaaatttt tgtaaaatat agtctaccag
     5821 caaagaaaca ttttgggttt gattacgata ttttaattga taaacccatt ctccaatcac
     5881 acttctcttg ctatcaaagt gaggatgccc taattcatca taacctaaca aaatactttt
     5941 ttgcatatga tggtctaaag caaatccttt tgtccagtta ccatttagtt ctatcgccat
     6001 ttcattgact ccttgtcaaa attaagtata acgagcaatc ctagcaagcc gtagcccgca
     6061 tgtagggtat gtgcggtacg cacgcacgcg ttcttcattt tccctgtaac cccaacccac
     6121 cgcatgcgcg ccttgcggca cacactctgc cgacggattc aaaggtcgtc tgaaaaaacg
     6181 ctcgcggcgt ttgttacatc tgccttgcga taaaggctgc ctgaaaaatt cccactgttg
     6241 atatttcggg cattctttct caaaccgcct gcaaccgcaa aatctgctga aacctactcc
     6301 ctcccccgtg ggggagggct ggggagaggg cattctccga atttcggcaa cctttcctaa
     6361 cactctaacc gccaagacac aagccttgcg gcttgttgcc ctctctctaa cgctttccca
     6421 cagggagaga ggacggggcg gctgttggaa ttaagggttc tgcaggaaaa acaggttgtg
     6481 cgggctgttt tttgtttttc agacggcctt tataaatgct caattcagtc ggctttatcc
     6541 tattattttc aataatccaa tcaaaatcag ccatctgcta tcgtgcagct tgcttttcca
     6601 attcttgcaa cacgagcagg atttctgtca gcacatcgtt tgtctgctgt aaaatctcgt
     6661 gattccaaaa ccgcagtacg gtaaagccca agctgttgag atatgccgtc cgcgcgtggt
     6721 cgtatgcggc ttgttctgtg tgctgccctc cgtctgcttc gacaatcaac ttgggcgtta
     6781 cgcacataaa atcaacaata tagtttccca tcggctgctg gcggcggaat ttatagccgt
     6841 tcagacggcc tgcccgcagg tgctgccaca attttgcttc cgcctcgctc atttcttggc
     6901 gcatggcttt ggcgcgttga tgcaggacgg ggttgtcggc ggttaaaagt ttctcgtgcg
     6961 tgctcattct gttgtcgggt tactgtttgt gtaagcctta cagtttgttg ccctctctct
     7021 gactctctcc catgggagag aggacgtggt ggcggttggg attaagggtt ctgcaaggaa
     7081 aacaggttgt gcaggctgct ttttattttt cagatgacct gaaacaacag ctgcaaatct
     7141 aagttgcctt tcaaatcgcc tgcaaccgca aaatctgctg caaccttctc cctcccccgt
     7201 gggggagggc tggggagagg gcattcttcg aatttcggca accttcccca acaccctaac
     7261 cgcccaaaca caagccttgc tgtttgttgc cctctctcta acgctttccc acagggagac
     7321 aggacggggc ggctgtagtg attaagggtt ttgcaaggaa aacaaggaaa acaagttgta
     7381 caggctgttt tttatttttc aagcgacctg aaatcaacgc ttgtaaatcc aagtcgcttt
     7441 tcaaactgcc tgcaaccgca aaatctgctg caacctaccc cctcccccgt gggggagggc
     7501 tggggagagg gcattttcca agttgcggca atctttccca aatcccttag tcttctaaaa
     7561 tacaagcctt acggttgttg ccaaatgaaa cttcttccat acccaaaact tgcgtgtaaa
     7621 acgcaatggt tcggtcaatg tcggcaacgg tcagtaccag atggtcgagt gcgctaatct
     7681 tcatttcccg ctttcttttt caggcggcgg cagtgccgtc tgaaagccat tatgcccgtt
     7741 ttgccgcttt catattgcca atgaatttgt caaacaaata gccgacatct tgcggaccgg
     7801 ggctggcttc ggggtggcct tggaagcaga acacaggttt gtcggtcagc tcgatgcctt
     7861 gcaaagtgtt gtcaaacaag gatttgtggg taatgcgtgc gttagcgggc agggtgtcgg
     7921 catcaacggc aaaaccgtgg ttttggctgg tgatgacgac tttgccgctg tccaaatctt
     7981 gcacagggtg gttcgcaccg tggtggctga agcgcatttt cagggttttc gcgccgatag
     8041 cgaggctgat gagctggtgt cccaagcaga tgccgaaaat cggtttgccg ctttccatca
     8101 gtttttgcac ggcttcgatg gcgtaggtgc aaggctcggg gtcgccgggg ccgttggaca
     8161 ggaacacgcc gtcgggattg agtgccaaca cgtcttgtgc gctcgtttgg gcggggacga
     8221 cggtcaggcg gcagccgcgc gaggcgagca tacgcaggat gttggttttc acgccgaaat
     8281 cgtaggcgac gacgtggtaa ggctgttcgt caggggtaac gaaacccttg cccaattccc
     8341 attcgccttc cgtccattcg taagtttccg tgcaggaaac ttcttttgcc aagtctttgc
     8401 cgaccatact gccgaacgcg gcgatgagtt cttgcgcttt ttcgattgtg gcatccgcac
     8461 cggtcagaat cgcgccgcct tgcgcgcctt tttcgcgcaa cagcgtggtc aggcggcggg
     8521 tatcgatgtc ggcgatggcg acggttttgt tgcgtaccag atagtcgtgc aggctttcgg
     8581 aggcgcggaa gttgctgtgc aagagcggca ggtcgcggat aatcaggccg gcggcataaa
     8641 cgctgcggct ttcttcatct tcggcgttgg tgccggtgtt gccgatgtgt gggtaggtga
     8701 gggtaacgat ttgtttgcag taggacgggt cggtcaggat ttcctgatag ccagtcatcg
     8761 aagtattgaa cacgacttcg ccggaagtcg aaccttcgta accgattgat gtgccgtgga
     8821 atacgctgcc gtcagcgagg acgaggaggg cgggggtgct catgatggga atcctgtttt
     8881 taataaaatt cttgacaatg ccgtctgaag ggacttcggc agggggcatc cggtcggaaa
     8941 aatccggtca aaaaaaaaca cgccgcgcaa gatagacgcg taacgtgctt ttcgggcaag
     9001 tgcctatacc ctgaaagcag catattttaa gacgaaacat acccgatttc aagcgcaaac
     9061 gcggcaggcg ggctgatatg ttcgcgcgaa aacggaaatc cgtggcgtaa aaccgcccgc
     9121 tctgcgccca ctatgcccgt tacgcatttc cccacgtccg tccggcgaaa ctatgggaat
     9181 acccgaa
//
LOCUS       DAWKST010000076         9169 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.076, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000076 DAWKST010000000
VERSION     DAWKST010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9169
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.076"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..275
                     /locus_tag="WJL87_001237"
     CDS             <1..275
                     /locus_tag="WJL87_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HEZ7799662.1"
                     /translation="DLNQMYLEDGSLSVQILGRGFAWLDTGTHESLHEAASFVQTVQN
                     IQNLHIACLEEIAWRNGWLTKKDVETRAKPLEKTAYGQYLLRLIGK"
     gene            320..859
                     /gene="rfbC"
                     /locus_tag="WJL87_001238"
     CDS             320..859
                     /gene="rfbC"
                     /locus_tag="WJL87_001238"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ7799663.1"
                     /translation="MNIIDTALPDVKLLKPQVFKDGRGFFMETFRDEWFKTQVCERTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAIDMREGSPTFGKWAGATLS
                     AQNQCQLWIPEGFAHGFYVLSDTTELTYKCTDYYKPEAEQVLIWNDPTVGIGWPLQTA
                     PNLSPKDLAGKVLAEAVTF"
     gene            883..2985
                     /locus_tag="WJL87_001239"
     CDS             883..2985
                     /locus_tag="WJL87_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ7799664.1"
                     /translation="MFLFSDGLQSINNNNRRKRIVKNAYIPSRGIRKIPHLFTLLPEF
                     HICKDGKGAEAVIGWGLRPTTHKARAFAAEHQLPFIALEDGFLRSLGLGVAGYPPYSI
                     VYDDIGIYYDTTRPSRLEQLILAANMPSEMLNQAQQAIDLILTHRLSKYNHALDFSDG
                     LVQDSKNIVLVIDQTFGDMAIQYGGADEATFRQMFQTALAENPDAEIWVKTHPDVLSG
                     KKQGYLTDLAQQPRVRLLAEDINPISLLQNIDKVYCVTSQMGFEALLCGKPLTTFGLP
                     WYAGWGVSDDRHPKIDSLVQTQRRAPRNLLQLFAAAYLQYSRYLNPNTGEAGSLFDVI
                     DYLATVKRKNDKLRGELYCVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLARVKLSDD
                     ARILAWGNGKEAIVRFAEQHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMGIYFNA
                     EAPSRLEHILQNQNFDDQDFQTALKLQKMLTENHISKYNVGNSDFTAPSTDKTVILVP
                     GQVEDDASIRYGSPQIYHNLDLLRTVRERHPDAYIIYKPHPDVVSGNRIGHISPEDAA
                     RYADQTAEQADILTCLQYADEIHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLTQDLL
                     PIPRRSRRLELWQLIAGTLIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNNGLHR
                     GCFAKKLGKIKQLYRSFK"
     gene            3122..4381
                     /locus_tag="WJL87_001240"
     CDS             3122..4381
                     /locus_tag="WJL87_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HEZ7799665.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFSSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSNSSTPVI"
     gene            4749..6155
                     /locus_tag="WJL87_001241"
     CDS             4749..6155
                     /locus_tag="WJL87_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ7799666.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYAEAAFVLG
                     NHLLQNGQPEQALSWLEAAAAQRHPKALFSLLQQREHNGTPTGQLLNDYAWLGEQGHS
                     EAQLILMRYHAQRNDPQSLYWAELAAARYAAPAYYHLARHHQRQGDVETAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSLNHAQAEHYLEPAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAADKEQAEAQSKLAQYALTGELSERDPFQAARYAKAAAEKNHPEALKIMGD
                     LYRYGLGIKADNHIAQDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAEAQACAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGIGCHVPTACYWLQAAISNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            6373..7587
                     /gene="gltS"
                     /locus_tag="WJL87_001242"
     CDS             6373..7587
                     /gene="gltS"
                     /locus_tag="WJL87_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ7799667.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     VAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYIFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            7686..8702
                     /locus_tag="WJL87_001243"
     CDS             7686..8702
                     /locus_tag="WJL87_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ7799668.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSVALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDADVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            8713..8910
                     /locus_tag="WJL87_001244"
     CDS             8713..8910
                     /locus_tag="WJL87_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799669.1"
                     /translation="MNTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAV
                     RLGENGFTEEQMENIANLFGR"
     gene            8928..9077
                     /locus_tag="WJL87_001245"
     CDS             8928..9077
                     /locus_tag="WJL87_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799670.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVAYADVSTRRTIYKAL
                     PYVPS"
BASE COUNT         2372 a         2621 c         2140 g         2036 t
ORIGIN      
        1 ccgacctcaa ccaaatgtat ttggaagacg gttcattgtc cgtccaaata ttaggacgcg
       61 gtttcgcatg gttggacacc ggcacccacg agagcctgca cgaagccgct tcattcgtcc
      121 aaaccgtgca aaacatccaa aacctgcaca tcgcctgcct ggaagaaatc gcttggcgca
      181 acggttggct gacgaaaaaa gatgtggaaa cacgggcaaa gcctttggaa aaaaccgcct
      241 acgggcaata cctgctgcgc ctgatcggca aataaatgcc gtctgaacca ctttcttcag
      301 acggcataca gagaaaatca tgaacatcat cgacaccgcc ctccccgacg taaaactttt
      361 aaaaccgcaa gtcttcaaag acgggcgcgg cttttttatg gaaaccttcc gcgacgagtg
      421 gtttaaaacc caagtctgcg aacgcacctt cgtgcaggaa aaccactcca aatccggcaa
      481 aggcgtattg cgcggcctgc actaccaaac cgaaaacaca caaggcaaac tcgtacgcgt
      541 agttgtcggc gaagtgttcg acgtggccat cgatatgcgc gaaggttcgc cgactttcgg
      601 caaatgggcg ggcgcaaccc tgtccgcgca aaaccaatgc cagctttgga tacccgaagg
      661 cttcgcgcac gggttttacg ttttgagcga cacgaccgaa cttacctaca aatgcacgga
      721 ctattacaag cctgaagccg aacaggtttt aatatggaac gacccgacag tcggcatagg
      781 ctggccgctt caaaccgcgc cgaacctgtc gcctaaagac ttggcaggca aagtattggc
      841 cgaagcagtc acgttttaag actattcagg ccgtctgaaa gaatgttcct cttttcagac
      901 ggcctacaat ccattaataa caataatcga cgaaaacgca ttgtgaaaaa cgcctacatc
      961 ccctctcgtg gcatccgcaa aatcccccat ctcttcaccc tattgcctga gtttcatatc
     1021 tgcaaggatg ggaaaggagc agaggctgtt atcggctggg gtttgcgccc gacgacacac
     1081 aaagcgcgtg cttttgccgc tgaacaccag cttcccttta ttgctttgga agacggcttt
     1141 ttacgctcgc ttggcctggg cgttgccggc tatcctccct attccatcgt ctatgacgac
     1201 atcggcatct attacgacac aacccgccca tcgcgtttgg agcagttgat tttagccgca
     1261 aacatgccgt ctgaaatgct taaccaagcg cagcaggcca ttgatttgat tttgacgcac
     1321 cgtttgtcta aatataacca tgctctggat ttttcagacg gcctcgtaca agacagcaaa
     1381 aacattgttt tggtaattga tcaaactttc ggcgatatgg cgattcaata tggcggcgca
     1441 gatgaagcca cattccgcca aatgtttcag acggccttag ccgaaaatcc tgatgcggaa
     1501 atctgggtca aaacccatcc cgatgtattg agcggcaaga aacagggtta ccttactgat
     1561 ttggcacagc agccgcgtgt gcgtttattg gctgaagaca tcaatccgat ttctttgttg
     1621 caaaacatcg ataaagttta ttgcgttacc tcgcaaatgg gttttgaggc gcttttgtgc
     1681 ggcaagccgc tgaccacttt cggcctgccg tggtatgccg gatggggcgt gagtgacgac
     1741 cgccatccta aaatcgacag ccttgttcaa acccaacgcc gcgccccccg caacttgctg
     1801 cagctcttcg ctgcagccta tctgcaatac agccgctacc tcaaccccaa taccggcgaa
     1861 gcaggcagcc tctttgatgt catcgactat ctggcgacgg tcaaacgcaa aaacgacaaa
     1921 ttgcgtggcg agttatattg cgtcggtatg tctttgtgga aacgcgcggt tgccaaaccg
     1981 ttctttaacg taccctcttg ccgtctgaaa tttatctctt ccacccaaaa actggcaagg
     2041 gtcaaactgt ccgacgatgc acgcatcctg gcttggggca acggcaaaga ggccatcgtc
     2101 cgctttgccg aacaacacca catccccctg ctgcgcatgg aagacggctt tatccgctcg
     2161 gtcggactcg gctccaactt agtgccgccg ctgtcgctcg ttaccgacga tatgggtatt
     2221 tacttcaatg ccgaagcccc atcccgcctt gaacacatcc tgcaaaacca aaacttcgac
     2281 gatcaagact ttcagacggc cttgaagttg caaaaaatgc tgaccgaaaa ccatatcagc
     2341 aaatacaacg tcggcaactc agactttacc gccccatcaa ccgacaaaac cgtgatcctc
     2401 gttcccggtc aggttgagga tgatgcgtct atccgctatg gttcgcccca aatctatcac
     2461 aatctggatt tgctccgtac cgtacgcgaa cgccatcccg atgcctatat catctacaaa
     2521 ccgcatcccg atgtagtcag cggtaaccgc atcggccata tttcccctga agatgctgca
     2581 cgatatgccg accaaaccgc cgaacaagcc gacatcctga cctgtctcca atacgcagac
     2641 gaaatacata ccatgacttc gctgaccggt tttgaagcct tgttgcgcgg caaaaaagtc
     2701 agctgctacg gcctgccttt ttacgcaggc tgggggctta cccaagatct gctccccatc
     2761 ccgcgccgta gccgcagact tgagctttgg cagctgattg ccggcacgct catccactat
     2821 cccgactaca tccaccccga aacccatcag gccataaatg cagaaaccgc agcccaaatc
     2881 ctgatacgac aaaaaaatat gcaaaaaaac aacaacggat tacatcgcgg gtgctttgcc
     2941 aaaaaattag gtaaaatcaa acaactatat cgatctttca aataaatacc atcaaagtta
     3001 acgatgcgtc ataaacttgc ctctattgcg gcatcattgc ctttgcatcg ttaattctct
     3061 tggcgtatgc ttgaaagttc aacctaaaac tattacataa aaaacaaaac cacattgcaa
     3121 catgaaacag accgtcctca aaaataacct gcaaaacctg cttgaaagcg cagaaaatat
     3181 cctgctgctt caaggccctg tcggcgattt ttttctgcgc cttgccgact ggctgactgc
     3241 aaacggcaaa accgtacata aattcaactt taatgcaggc gacgactatt tttatccgcc
     3301 cactcaagcg cataccgttg tttttaacga caactacgat gcctttcctg agtttttgca
     3361 agaatacatc actcaacatc acatccaggc cgttgtctgc tttggcgaca cacgccctta
     3421 tcacgtcatt gcaaaacgca ttgcaaacga aaaccaagcc agtttctggg cgtttgaaga
     3481 aggctatttc cgcccctact acatcacctt agaaaaagac ggcgtcaacg cattttcccc
     3541 gttgccgcgc cgtgccgact tttttcttga acaattccct aagcttgccc agcaagaata
     3601 taaagcgcca acgccggtac acggcggttt tacgcccatg gcaaaaaacg ctatccgtta
     3661 ctatatcgag ttgttccgca atccacgcaa ataccccgac tacatccacc accgcgcacc
     3721 caatgccggc cattacctca aaccgtggtc gctctccatc ctcaagcgtt tgaactacta
     3781 tattgaagac atccaaatcg ccaaacgtgt ggaagcaggc aaatacggca agttttttat
     3841 tgttccctta caggtattca acgacagcca agtccgtatc cattgcgact tttccagcgt
     3901 ccgcagcttc ctgctccatg ttttgagttc atttgccgag cacgcgcctg ccgataccaa
     3961 catcatcatc aagcatcatc cgatggaccg cggttttatc gactactggc gcgacattaa
     4021 acgctttatc aaagaacacc ccgaactcaa aggccgcgtg atttatgtcc atgatgtccc
     4081 cctgcccgtt ttcctgcgcc acggtctcgg catggtcacc atcaacagca ccagcggcct
     4141 gtccggcctg attcacaata tgccagttaa ggttctcggc cgtgcctatt atgatattcc
     4201 cggcattact gaccaaaata ccttggcaga attttggaat catccgacac cgcctgacaa
     4261 agagctgttc catgcctacc gaatgtacca cctcaacgtg acccaaatta acggcaactt
     4321 ctacagtcag gtgtttttcc ccaacaaaaa aacctccaac tcttccacac cagtaatctg
     4381 acttagcgaa ggaaagttca ggccgtctga aaacatttca gacggcctga aacaatcaat
     4441 accttagcta ctgccatgta aataaaacac aaaaatctgc atttatcatt aacaataaat
     4501 tacaaaaaca gtataatgac cgagctgcca tgagcgcata ccgactcaac ctgagccctt
     4561 tgtaacacac aaaatatgga tatatcccta ggcaaaacaa tataacaagc caaacatcct
     4621 aaagataagc cggcaaggca atacactcta taaaactgtg ccgagcaaaa tttttacaaa
     4681 gccctcaacc ggtatcgccg cccatatgcc gcagcatccg tcttccactt tatatccgcc
     4741 cgcaaaccat gaccgccgct cctgatatcc tctaccggca agccgccgcc cttttggaac
     4801 aatccaatac cgcccaagcc ctgcccctgt tgcaacaggc ggcagagcaa ggttatgcgg
     4861 aagctgcttt cgtattgggc aaccatctgc tgcaaaacgg ccaaccggag caggcacttt
     4921 catggttgga agccgccgcg gcccaacgcc atcccaaagc actcttctcc ctgctgcaac
     4981 aacgcgaaca caacggcacc ccgaccggac agcttctcaa cgactatgcc tggctgggtg
     5041 agcaggggca ctcagaagcc caattaatcc tcatgcgtta ccacgcgcaa cgcaacgatc
     5101 cacaatcgct ctactgggcg gaacttgctg ccgcccgata tgccgcacct gcgtattacc
     5161 atctggcacg ccatcatcaa cgccaaggcg acgttgaaac agccatcgaa caatacgaaa
     5221 aagcggcagc actcggcgta actgccgcct gctggcaact tggtcaaatc tacttctacg
     5281 gtacaggtgt cagcctcaac cacgcacaag ccgaacacta tctcgaacca gccgcacaag
     5341 ccggccacat cgccgcacaa acgctgctgg ctgaccttct tgccgcccaa cgcaaacctg
     5401 aagccttgga atggtatcgt cgtgccgccg ataaggaaca agcggaagca cagtctaagc
     5461 tggcccaata cgccctgacc ggcgaacttt ccgaacgcga tccgttccaa gcggcacgat
     5521 atgccaaagc cgctgccgag aaaaaccatc ctgaagccct gaaaatcatg ggcgacctct
     5581 accgctacgg tctcggtatc aaagccgaca accatatcgc gcaagattac taccaccgtg
     5641 ccgccgcgct gggttctgcc gccgcagcac aaaaactcat cagcgacgcc gcgctgtacc
     5701 atccgcaaca atacgaacaa atcaaaactg ccgccctgca acaacaacaa accgaaacca
     5761 tctaccgttt ggcggaagca caagcctgcg ccatcggccg tcccgccgac tacaatgccg
     5821 cgcgaaaaaa ttacatggaa gctgccgggt tccaccataa aaacgcagcg gcagccttag
     5881 gccgcatcta ccattacggc ctcggtacgg cgcaagatcc tcgggcggct gcacactggt
     5941 acgccattgc tgccgaacaa aaccaccctt ccgcccaata ccacctcgcc tgtttttact
     6001 atcacggaca aggtatcggc tgtcatgttc cgaccgcctg ctactggctg caggccgcca
     6061 tcagcaacgg ccacacttcg gccgaatcat taatatccct attagaacaa tggcgacgcg
     6121 aagcacacca tgccatcgga caaaaggccg tctgaaaaga tttacactcg cattttttga
     6181 caatctttaa ctattcccct aatatttgcc agttattttt cacggacacg ccattgtttt
     6241 catttctttc tgaaaacacc ttgtccgcgc atcaatacca tgacactcgg cggataacgc
     6301 caagcgttga aacacactac atccggaaca aaaacggatg ctcggaaaaa tatttctagg
     6361 aggtgaaaca acatggaatg ggaattcaac agttattaca cactgattgc cgccacgctc
     6421 gtgttgctgg ttggtaaatt tctggttcaa aaaatcaaat tcttacgaga cttcaatatt
     6481 cccgagccgg tcgctggcgg tttggttgcc gcgattatcc tgtttgcgct gcacgaggcg
     6541 tacggcgtga gcttcaaatt tgagaaaccg ctgcaaaatg cgtttatgct gatttttttc
     6601 acgtccatcg gcttgagcgc agatttttcc cgtttgaagg cgggcggttt gccgctggtg
     6661 gtttttaccg cgattgtggg cggatttatc ttggtgcaaa actttgtcgg ggtcggactg
     6721 gctacggctt tgggtttgga tccgctcatc ggtctgatta ccggttcggt gtcgctgacg
     6781 ggcggacatg gtacgtcagg tgcgtgggga cctaattttg aaacgcaata cggcttggtc
     6841 ggcgcaaccg gtttgggtat tgcatcggct actttcgggc tggtgttcgg cggcctgatc
     6901 ggcgggccgg ttgcgcgccg cctgatcaac aaaatgggcc gcaaaccggt tgaaaacaaa
     6961 aaacaggatc aggacgacaa cgcggacgac gtgttcgagc aggcaaaacg cacccgcctg
     7021 attacggcgg aatctgccgt tgaaacgctt gccatgtttg ccgcgtgttt ggcgtttgcc
     7081 gagattatgg acggcttcga caaagaatat ctgttcgacc tgcccaaatt cgtgtggtgt
     7141 ctgtttggcg gcgtggtcat ccgcaacatc ctcactgccg cattcaaggt caatatgttc
     7201 gaccgcgcca tcgatgtgtt cggcaatgct tcgctttcgc ttttcttggc aatggcgttg
     7261 ctgaatttga aactgtggga gctgaccggt ttggcggggc ctgtaaccgt catccttgcc
     7321 gtacaaaccg tggtgatggt tttgtacgcg acttttgtta cctatatctt tatggggcgc
     7381 gactatgatg cggcagtatt ggctgccggc cattgcggtt tcggcttggg tgcaacgccg
     7441 acggcggtgg caaatatgca gtccgtcacg catactttcg gcgcgtcgca taaggcgttt
     7501 ttgattgtgc ctatggtcgg cgcgttcttc gtcgatttga ttaatgccgc gattctcacc
     7561 ggttttgtga atttctttaa aggctgattt tccgcctttc cgacaaagca cctgcaaggt
     7621 ttaccgcctg caggtgcttt tgctatgata gccgctatcg gtctgcaccg tttggaagga
     7681 acatcatgta tcggaaactc attgcgctgc cgtttgccct gctgcttgcc gcttgcggca
     7741 gggaagaacc gcccaaggca ttggaatgcg ccaaccccgc cgtgttgcaa ggcatacgcg
     7801 gcaatattca ggaaacgctc acgcaggaag cgcgttcttt cgcgcgcgaa gacggcaggc
     7861 agtttgtcga tgccgacaaa attatcgccg ccgcctacgg tttggcgttt tctttggaac
     7921 acgcttcgga aacgcaggaa ggcgggcgca cgttctgtat cgccgatttg aacattaccg
     7981 tgccgtctga aacgcttgcc gatgccaagg caaacagccc cctgctgtac ggggaaaccg
     8041 ctttgtcgga tattgtgcgg cagaagacgg gcggcaatgt cgagtttaaa gacggcgtat
     8101 tgacggcagc cgtccgcttc ctgcccgtca aagacggtca gacggcattt gtcgacaaca
     8161 cggtcggtat ggcggcgcaa acgctgtctg tcgcgttgct gccttacggc gtgaagagca
     8221 tcgtgatgat agacggcaag gcggtaaaaa aagaagacgc ggtcaggatt ttgagcggaa
     8281 aagcccgtga agaagaaccg tccaaaccca cgcccgaaga cattttggaa cataatgccg
     8341 ccggagggga tgcagacgta ccccaagccg cagaaggcgc gcccgaaccg gaaatcctgc
     8401 atcctgacga cggcgagcgt gccgataccg ttaccgtatc acggggagaa gtggaagagg
     8461 cgcgcgtaca aaaccagcgt gcggaatccg aaattaccaa actttgggga ggactcgata
     8521 ccgacgtgca aaaagagttg gtcggcgaac aacgcaagtg ggcgcaggaa aaaatcagca
     8581 actgccgaca agccgccgcg caggcagacc ggcaggaata cgccgaatac ctcaaattgc
     8641 agtgcgacac gcggatgacg cgcgaacgga tacagtatct tcgcggctat tccatcgatt
     8701 aggggcaaac cgatgaatac cgtcccaaaa agcaggattc ccgtcaaacc gctgcccgaa
     8761 aaaaccacag acgaagccaa agtcgaaaaa tggcggcagc tcggtgcgga acacggtttg
     8821 tcgggcgaat gggcagttgc cgtcagattg ggcgaaaacg gttttaccga agaacagatg
     8881 gaaaatatcg ccaacctgtt cggcagataa agagaaaatt gacggaaatg ccgtctgaaa
     8941 ccctgttatc ggtttcagac ggcattttga ccaatacggt acgcaggcgc aaaacagccg
     9001 gcttttcctg tgttgcctat gctgatgttt caacacgcag gacgatatat aaagcgttgc
     9061 cctatgtgcc gtcctgattc ggaaagggtt acgcccctcc taaatatagt ggattaacaa
     9121 aaaccagtac ggcgttgcct cgccttagct caaagagaac gattcttta
//
LOCUS       DAWKST010000077         8964 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.077, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000077 DAWKST010000000
VERSION     DAWKST010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.077"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            129..971
                     /locus_tag="WJL87_001246"
     CDS             129..971
                     /locus_tag="WJL87_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ7799671.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
     gene            1000..1464
                     /gene="nrdR"
                     /locus_tag="WJL87_001247"
     CDS             1000..1464
                     /gene="nrdR"
                     /locus_tag="WJL87_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ7799672.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPSCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSTELADMVLKELLEHNTEAAIRFAALHKRFDSPEHFASWLAQAVKTGGKA"
     gene            1496..2605
                     /gene="ribD"
                     /locus_tag="WJL87_001248"
     CDS             1496..2605
                     /gene="ribD"
                     /locus_tag="WJL87_001248"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ7799673.1"
                     /translation="MFSDTDISMMETALRLAALGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPLVAGKGLALLEAAGIKTECGLLEHQARKLNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPPNSHLVTDGQSPTYIATLERNEDRLHPYREHAHVRILMPSETADGK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAEDLADEIVLYRSPKILGGGSGLFCLP
                     ENRAALSAPPLWTPVSSEILGHDIKTVFRKNGNAF"
     gene            2800..4221
                     /locus_tag="WJL87_001249"
     CDS             2800..4221
                     /locus_tag="WJL87_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEZ7799674.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSHPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLPLT
                     VGLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRHAPFSPAVLHRGLRYGIP
                     IALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFR
                     AIEENAPPARLSATAESAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPP
                     LFCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACATSFW
                     LFFVFKTESSCRLWQPLKRLPLYTHTLLCLTSSAAYTCFGTPANYPLFAGVWAVYLAG
                     CILRHWKDLHKLFHYLKKQGFPL"
     gene            4218..5291
                     /locus_tag="WJL87_001250"
     CDS             4218..5291
                     /locus_tag="WJL87_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ7799675.1"
                     /translation="MKIILTTSMSGLGGTETATVRLGRLLKRRGHDIILASSDGPFVG
                     EAQASGIRWQPVDFYRGGLAGYLKSTFAYARMLRREQPDIIDCQMARVVPACALAAKI
                     VSPKTKIIYHSHGLDAATYPKIAKLFDKLGVYIIGNCKHEREKLIRHGFPAGRIAYAY
                     NALPPPPEFHFRKTKKECAVLGTLSRLDTVRAVHLMLDILKKMVDRGMPVRLNVAGIG
                     EEMDNLKAQAKRLGIEDKVTFLGGVRDLTGYFKEVDILVNTPHCVGDHGAGVGNNILE
                     AGLYDTPVVTYNMAGISEMVITGQTGYCIPFGDDEAFIEAFDTLIKHPELRSQMGKAL
                     HKHVETLCSDDEIYRTTMAAYEM"
     gene            5305..6477
                     /locus_tag="WJL87_001251"
     CDS             5305..6477
                     /locus_tag="WJL87_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7799676.1"
                     /translation="MNITIVAPYCSLPSEPHFNRFWYLAELLSQSHDVLLITSNFKHY
                     DKSFRRPEDAEAASQGRLKVMLLEESGYSKNVSLGRVTSHHRFVKHFEKWLENCRPGE
                     QDVVFSAYPLIATNLLLGKHKARLGYKLIVDVQDVWPESFSSVVPFLKKVPHKLLPFA
                     SRANRAYRYADALVAVSQTYLDRAKETNPNVPGEVIYIGADFPKLDAAPAKDFGDDKT
                     RFFYLGTLSYSYDVETVCKGVRKLLDDGENVELHIMGGGPDLDRLKQYACDGIKFYGY
                     IPYAEMMSVAKGCDIAINAIQSYSMASITNKLSDYMALQKPILNSQVNDEVAEVLTLL
                     PHADYRSGDVDGFVQADKDILKRKNDPVQSEEIVRRFKRDVAYQKIVNLIERLAHE"
     gene            6470..7087
                     /locus_tag="WJL87_001252"
     CDS             6470..7087
                     /locus_tag="WJL87_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_046551259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEZ7799677.1"
                     /translation="MNKFFKRLFDIIASASGLIILSPVFLILVYLIRKNLGSPVFFIQ
                     ERPGKDGRPFKMIKFRSMRDAIDKDGNPLPDSERLTPFGKKLRAASLDELPELWNVLK
                     GDMSLVGPRPLLMQYLPLYDNFQNRRHEMKPGVTGWAQVNGRNAISWDEKFAHDVWYI
                     DNFSFWLDIKILFLTVKKVFVKEGISAEGEATMPYFTGNKTDEKK"
     gene            7074..8048
                     /locus_tag="WJL87_001253"
     CDS             7074..8048
                     /locus_tag="WJL87_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEZ7799678.1"
                     /translation="MKKNNILILSAGRRVELVQDFQTEAARFSDGISVFATDLNPRMS
                     SACHVSDGSFEVPPISADSYIDSIFNLAVRENIGLIIPTIDTELQKLADERERFEAAS
                     VHIVVSDSDFIAQCRDKRKTATLFARYGIRSPEIYDRKKLVFPCFAKPYDGSRAIGAK
                     RIDTPADLTPEVLADTKLMFCQFIDIENKFSEFTVDMYYDRQCRLKCAIPRKRLEVRS
                     GEISKGVTCKNSLYQTLLEKMSVLQGARGCITAQFFYNEETGEFYGVEINPRFGGGFP
                     LTYAAGGNYPSRLMREYLGDEEIPFSDDWENNLIMLRYDAKVLVHEND"
     gene            8035..8721
                     /locus_tag="WJL87_001254"
     CDS             8035..8721
                     /locus_tag="WJL87_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ7799679.1"
                     /translation="MKTINPETTVIVFDLDDTLYSEYEYKLSGIRSVIDTVAALYPDW
                     NSDNLWRNIDPDGKDWLDKLCRHCGFNESEKQVLLWQYRLHRPTLTPYAPPDFLSELT
                     APFAARALITDGRSLTQRLKLEALGLSSLFDDILISEACSSEKPDSKRFRHLQDKYAD
                     KAGCFIYIGDNISKDFIAPNTLGWITIGLLPSGQNIHRHQPESFNTEYHPDFWVNSFQ
                     DLVPIIDSNT"
BASE COUNT         2034 a         2805 c         2203 g         1922 t
ORIGIN      
        1 atcttaagtt tccgtctttg ttttctgttt tcgagggaat aatggggaac aagccgtatt
       61 tcagacggca ttttcagttc ggggtataat ccgaatactt gcgaccatct gaatcattgg
      121 gacaaaccat gtgtcaactg ctgggcatga actgcaatac gccgaccgat attatgtttt
      181 cctttgaagg cttccgccgc aggggcggca ttaccgacca ccatgccgac ggtttcggca
      241 tcggcttttt cgaaggcaaa ggcgtgcgcc tgttccatga cgacaaaccc agtgccaatt
      301 cgcccgtcgc cgacctcgtg cgcgcctacc agattaaatc ggaaaacgtc gtcgcccata
      361 tccgcaaagc atcacaagga caaacctcgc tggcgaacac ccatcccttt atgcgcgaaa
      421 tgtggggcgg ctactggctg tttgcccaca acggacattt gattgatttt ttccccgaac
      481 agggcgaatt tttccacccc gtcggcacaa ccgattccga acgcgcgttc tgccacatcc
      541 tcaaccgcct gcgcacccgc tttgccgccc gtcccgacga cgacacgctg tttgacgcga
      601 ttgcggggct gacgcacgaa atccgcaagt tcgggctgtt taactttatg ctttcagacg
      661 gcattgccct gtttgcccac gccagcacac tgctgcacta catcgtccgc caagccccgt
      721 tcggcaaggc gcgcctgctc gacgacgacg tgatggtcga ttttgccgaa gtaaccacgc
      781 cctccgaccg cgtcgccgtt atcgccaccc tgccactgac ccgcgacgaa tcatggtccc
      841 aacttgccgt ggacgaactg gtcatgttcc gcgaaggcaa catcgtccga cacgaccgtc
      901 ccgaaaaccc cgtctatatg agtgccgaag aaggtctgga aatcgcccgc gccgccggcg
      961 tatcggtttg acgttcagac ggcatcagga agaaacgcta tgaaatgccc gttttgcgcc
     1021 caccccgaca cccgcgtcgc cgattcgcgc ctgatggaag aacgcaacgc cgtgcgccgc
     1081 cgccgccact gccccagctg cggcaaacgc ttcggcacgc tcgaaaccgc cgaactcaaa
     1141 atgcccgccg tcatcggtcc ggacaaaaaa cgttcgccct ttaatgcaca acgcctccgc
     1201 aacgacctga ccgccgccgc ccgaaaatcc gccctgacac ccgaacagat cgacgaaacc
     1261 gtccgcctga cggaacacag gctctacact tcgggtcagc gcgacatccc ctccaccgaa
     1321 cttgccgaca tggtgctaaa agagctgctt gagcacaaca cggaagccgc catacgcttc
     1381 gccgcactgc acaaacgctt cgacagcccg gaacatttcg cctcgtggct ggcgcaagcc
     1441 gtcaaaacag gcggcaaagc ctgattcccc caacccatac tgatacggta tccctatgtt
     1501 ttcggacaca gatatatcca tgatggaaac cgccctccga cttgccgctt tggggcgttt
     1561 ttccacttcg cccaatccgc gcgtcggctg cgttatcgca cacggcagcc aaattgtcgg
     1621 gcagggcttc cacgtcaaag cgggcgaacc ccatgccgaa gtccacgccc tgcgtcaggc
     1681 gggcgaaatg gcacaaggcg cgaccgcctt tgttaccctc gaaccgtgca gccattacgg
     1741 acgcacaccg ccctgcgccg aagctctgct ccgttccggc gtaacacgcg tcgtcgccgc
     1801 catgcgcgac cccaacccgc tggttgcagg caaagggctt gccctgctcg aagcagcagg
     1861 catcaagacg gaatgcggtt tactcgaaca tcaggcaagg aaactcaacc gaggcttcct
     1921 gtcgcgcatc gaacgccgcc gcccctttgt ccgcctcaaa tgcgccgttt cgctggacgg
     1981 caaaaccgcc ctttcagacg gcagcagctt ttggattacc ggcgaagacg cgcgtgccga
     2041 cgtacaggtt ttgcgtgccg aaagctgcgc ggtgctgacc ggcatcggca cggtgttggc
     2101 ggacaatccc cggctcaacg tccgcgcttt tccaactttg cgccaacccg cacgcatcgt
     2161 tttagacagc cgcctgcgcc tgcccccgaa cagccatttg gttaccgacg gacaatctcc
     2221 gacctacatc gccacactcg aacgcaacga agacagactg cacccctatc gggaacacgc
     2281 acacgtccgc atcctgatgc cgtctgaaac ggcagacggc aaaatcgacc tgcaccacct
     2341 gatgcgcctc cttgccgacg aaggtttcgg cgaaatcatg gtcgaagcag gctccgaact
     2401 cacatccgca tttttggcag aagatttggc ggacgaaatc gttttatacc gctcgcccaa
     2461 aatcctcggc ggcggcagcg gtttattttg cctgcccgaa aaccgcgccg ccctttccgc
     2521 accgcccttg tggacacccg tttcaagcga aatcctcgga cacgacatca aaaccgtgtt
     2581 ccggaaaaac ggcaacgcct tttaaagggt ttgcgccgtt tcactatata ataacgccga
     2641 taaaaaacgg ccccgttcag accgccgccc gcccgaaaaa acgcaacccg gactgccgca
     2701 ccccgccggc agccgcgacg gtctgaaagc cgtcaaattc cgatcaagaa aggcttcaga
     2761 cggcacaggc agcatcccgc cgccgcccgg acatcaaaaa tggacacaaa agaaatcctc
     2821 ggctacgcgg caggctcgat cggcagcgcg gttttagccg tcatcatcct gccgctgctg
     2881 tcgtggtatt tccccgccga cgacatcggg cgcatcgtgc tgatgcagac ggcggcgggg
     2941 ctgacggtgt cggtgttgtg cctcgggctg gatcaggcat acgtccgcga atactatgcc
     3001 gccgccgaca aagacacctt gttcaaaacc ctgttcctgc cgccgctgct gtctgccgcc
     3061 gcgatagccg ccctgctgct ttcccacccg tccctgccgt ctgaaatcct gttttcactc
     3121 gacgatgccg ccgccggcat cgggctggtg ctgtttgaac tgagcttcct gcccatccgc
     3181 tttctcttac tggttttgcg tatggaagga cgcgcccttg ccttttcgtc cgcgcaactc
     3241 gtgcccaagc tcgccatcct gctgctgctg ccgctgacgg tcgggctgct gcactttcca
     3301 gcgaacaccg ccgtcctgac cgccgtttac gcgctggcaa accttgccgc cgccgccttt
     3361 ttgctgtttc aaaaccgatg ccgtctgaag gccgtccggc acgcaccgtt ttcgcccgcc
     3421 gtcctgcacc gggggctgcg ctacggcata ccgatcgcac tgagcagcat cgcctattgg
     3481 gggctggcat ccgccgaccg tttgttcctg aaaaaatatg ccggcctgga acagctcggc
     3541 gtttattcga tgggtatttc gttcggcggg gcggcattat tgttccaaag catcttttca
     3601 acggtctgga caccgtatat tttccgcgca atcgaagaaa acgccccgcc cgcccgcctc
     3661 tcggcaacgg cagaatccgc cgccgccctg cttgcctccg ccctctgcct gaccggcatt
     3721 ttctcgcccc tcgcctccct cctgctgccg gaaaactacg ctgccgtccg gtttatcgtc
     3781 gtatcgtgta tgctgccgcc gctgttttgc acgctggtag aaatcagcgg catcggtttg
     3841 aacgtcgtcc gaaaaacacg cccgatcgcg ctcgccacct tgggcgcgct ggcggcaaac
     3901 ctgctgctgc tggggcttgc cgtaccgtcc ggcggcgcgc gcggcgcggc ggttgcctgt
     3961 gccacctcat tctggttgtt ttttgttttc aagacagaaa gctcctgtcg cctgtggcag
     4021 ccgctcaaac gcctgccgct ttatacgcac acattgctct gcctgacctc ctcggcggcc
     4081 tacacctgct tcggcacacc ggcaaactac cccctgtttg ccggcgtatg ggcggtatat
     4141 ctggcaggct gcatcctgcg ccactggaaa gatttgcaca aactgtttca ttatttgaaa
     4201 aaacaagggt ttccattatg aaaatcatcc tgaccacttc tatgtcgggc ttgggcggca
     4261 cggaaaccgc caccgtccgc ctcgggcggc tgctcaaacg gcgcgggcac gacatcatcc
     4321 ttgcttcttc ggacgggcct tttgtcggag aggcgcaagc atcgggcatc cgttggcagc
     4381 cggttgattt ttaccggggc gggcttgccg gctacctcaa aagcaccttt gcctacgccc
     4441 ggatgctgcg ccgcgaacaa cccgacatca tcgactgcca aatggcgcgc gttgttccag
     4501 cctgcgccct tgccgccaaa atcgtttcgc cgaaaaccaa aatcatctac cactcgcacg
     4561 gtttggacgc ggcaacctac cccaaaatcg ccaagctctt cgacaagctg ggcgtgtaca
     4621 tcatcggcaa ctgtaaacac gagcgcgaaa aactcatccg ccacggcttc cccgccggcc
     4681 ggattgccta cgcctacaac gcactccccc cccccccgga atttcatttc cggaaaacca
     4741 aaaaagaatg cgccgtactc ggcacacttt cccgtttgga caccgtccgc gccgtgcatc
     4801 tgatgttgga tattttgaag aaaatggttg accgcggtat gcctgtacgc ctcaacgttg
     4861 caggtatcgg cgaagaaatg gacaacctca aagcccaagc caaacgtttg ggcattgaag
     4921 acaaagtcac cttcctcggc ggcgtccgcg atttgaccgg ctacttcaaa gaagtcgata
     4981 ttttggtgaa cacgccgcat tgcgtgggcg atcacggcgc aggtgtcggc aacaatattt
     5041 tggaagccgg cctttacgac acgcctgtcg tgacctacaa catggcgggc atttccgaaa
     5101 tggtcattac cggccaaacc ggctactgca ttcctttcgg cgacgacgaa gcatttatcg
     5161 aagccttcga tacactcatc aagcatccag agttgcgcag ccaaatgggc aaagccctgc
     5221 acaaacatgt cgaaacctta tgctccgacg acgaaatcta ccgcaccacc atggctgcgt
     5281 acgaaatgta aggccgtctg aaacatgaac attaccatcg tcgcccctta ttgctcgctg
     5341 ccgtccgagc cgcatttcaa ccgcttctgg tatcttgccg agctgttgtc gcagtcgcat
     5401 gacgtgttgc tgattaccag caacttcaag cactacgaca aatctttcag acggcccgaa
     5461 gatgccgagg ccgcctcaca aggccgtctg aaagtcatgc tgttggaaga aagcggatac
     5521 agcaaaaacg tgtctttagg acgcgtgacc agccatcacc gcttcgtcaa acattttgaa
     5581 aaatggttgg aaaactgccg tccgggcgaa caagacgtcg tcttttccgc ctatccgctg
     5641 attgccacca acctgctgtt gggcaaacac aaagcgcgtt tgggttacaa actgattgtc
     5701 gatgtgcagg acgtatggcc ggagtctttc tcctcggtcg taccgttttt gaaaaaagta
     5761 ccgcacaagc tgctgccctt tgcttcacgc gccaaccggg cctaccgcta cgccgacgcg
     5821 ctggttgccg tatcgcagac ttatctcgac cgcgccaaag aaaccaatcc gaacgtaccc
     5881 ggcgaagtca tctatattgg tgctgacttt cccaaactcg atgccgcacc tgccaaagat
     5941 tttggcgacg acaaaacccg ctttttctac ttgggcacgc tcagttacag ctatgacgtg
     6001 gaaaccgtgt gcaaaggcgt tcggaaactt ttggacgacg gcgaaaatgt agagctgcac
     6061 atcatgggcg gcggccccga tttggacagg ctcaaacaat atgcctgcga cggcatcaag
     6121 ttttacggct acattcctta cgccgaaatg atgtcggtcg ccaagggctg cgacattgcc
     6181 atcaacgcca tccagtccta ttcaatggca tcgattacca acaaactctc cgactatatg
     6241 gctttgcaaa aaccgattct gaacagccag gtcaacgacg aagttgccga agtccttacc
     6301 ctgctaccgc atgcggacta ccgctccggc gatgtggacg gtttcgttca agccgataaa
     6361 gatattttga agcgcaaaaa cgatcctgtt cagtccgaag aaatcgtccg ccgcttcaaa
     6421 cgcgacgttg cctatcaaaa aatcgtcaac ctgattgaaa gattagctca tgaataaatt
     6481 tttcaaacgc ctgtttgaca ttattgcctc cgcctcaggg ctaattatcc tgtcgcctgt
     6541 gtttttgatt ttggtgtacc tcatccgcaa aaacttaggc tcgcccgtgt tcttcatcca
     6601 agagcggccg ggcaaggatg gcaggccgtt taaaatgatt aaattccgct ccatgcgcga
     6661 tgcaatcgat aaagacggca acccgctgcc cgacagcgag cggctcacac ccttcggcaa
     6721 aaaactgcgt gccgctagtt tggacgaact gcctgaatta tggaatgtct taaaaggcga
     6781 catgagcctg gtcggcccgc gcccgctgct gatgcaatat ctgccgctgt acgacaactt
     6841 ccaaaaccgc cgccacgaaa tgaaaccggg cgtgaccggt tgggcgcaag tcaatggccg
     6901 caacgccatt tcatgggatg aaaaattcgc ccacgatgtt tggtacatcg acaacttcag
     6961 cttttggctg gatatcaaaa ttttgttcct gacggttaaa aaagtcttcg tcaaagaagg
     7021 catctcagcc gaaggagaag ccaccatgcc ttatttcacg ggtaataaaa ctgatgaaaa
     7081 aaaataatat actgattctg tcggcaggtc gccgtgtcga attggtgcag gacttccaaa
     7141 ccgaagccgc acgtttttca gacggcatca gcgtttttgc cactgacctc aacccccgca
     7201 tgtcttctgc ctgccatgtt tcggacggtt catttgaagt gccgccgatt agtgccgatt
     7261 catatatcga cagcattttc aatctggcgg tacgcgaaaa tatcggttta atcatcccaa
     7321 ccatagacac cgaactgcaa aagctggcag acgagcgcga acgctttgaa gcggcaagtg
     7381 tccacatcgt cgtttccgat tcggacttca tcgcacaatg ccgcgacaaa cggaaaaccg
     7441 ccaccttgtt tgcccgctac ggcatacgct cacccgaaat ttacgaccgc aagaagttgg
     7501 ttttcccttg cttcgccaag ccttatgacg gcagccgcgc catcggtgca aaacggattg
     7561 acacacctgc cgacctgaca ccggaagtct tggcagacac caaattaatg ttctgtcagt
     7621 ttatcgacat cgaaaataaa ttttccgagt ttaccgtcga tatgtattac gaccgtcaat
     7681 gccgtctgaa atgcgccatc ccgcgaaaac gcttggaagt gcgctccggc gaaatcagca
     7741 aaggcgttac ctgcaaaaac agcctctacc aaacgctgct ggaaaaaatg tcggtattgc
     7801 agggcgcgcg cggctgtatc acggcgcagt tcttctacaa cgaagaaacc ggcgaattct
     7861 acggcgtgga aatcaacccc cgcttcggcg gtggcttccc gctgacgtat gccgcaggcg
     7921 gcaattatcc gagccggttg atgcgcgaat acctcggcga cgaagaaatc ccgttttcag
     7981 acgactggga aaacaacctc attatgttgc gctacgatgc caaggtcttg gttcatgaaa
     8041 acgattaatc ctgaaaccac cgtcatcgtc ttcgacttgg acgataccct gtattcggaa
     8101 tacgaataca agctctccgg tatccgttcg gtcatcgata ccgttgccgc actgtatccc
     8161 gattggaatt cagacaacct atggcgcaac atcgaccccg acggcaaaga ttggctggac
     8221 aaattgtgcc gccactgtgg ttttaatgaa tcggaaaaac aggttctttt gtggcaatac
     8281 cggctgcacc gcccgacgct gactccgtat gctccgccgg atttcctgtc cgaactgacc
     8341 gcgcccttcg ccgcacgcgc gctgattacc gacggcagaa gcctgaccca acgcctgaag
     8401 ctggaagcct tgggtctgtc ttccctgttt gacgacatcc tcatctctga agcctgctct
     8461 tcggaaaagc ccgacagcaa acgcttccgc catcttcaag acaaatatgc cgacaaggca
     8521 ggctgcttca tctatatcgg cgacaacatt tccaaagact ttatcgcgcc gaacactttg
     8581 ggctggataa ccataggatt gcttccctcc ggacagaaca tccaccgcca ccagcctgaa
     8641 agttttaaca cagaatatca tcccgacttt tgggttaact cctttcaaga tttagttcct
     8701 ataattgatt caaatactta accattacat aatcaaacat gcactcacaa tattaaaagg
     8761 actatttcat gacaaaatat tattcatggg gcgtaaacgg ctttccgccc tacgttttcg
     8821 acaccatttg gaaacgatgg aagttgaatg ggcgcggggg cggttatcgt gtgcgacatc
     8881 ccggacggca tgaccgtcgc gggcaatccg gcaaagccgc tgccgcgcaa aaaccccaag
     8941 accgggacgg cataagcgat taaa
//
LOCUS       DAWKST010000078         8922 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.078, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000078 DAWKST010000000
VERSION     DAWKST010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8922
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.078"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            188..622
                     /locus_tag="WJL87_001255"
     CDS             188..622
                     /locus_tag="WJL87_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ7799680.1"
                     /translation="MNFALSVITFTLASFLPVPPAEAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(671..2014)
                     /locus_tag="WJL87_001256"
     CDS             complement(671..2014)
                     /locus_tag="WJL87_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ7799681.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLVSDGIPLAGAWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(2019..2672)
                     /locus_tag="WJL87_001257"
     CDS             complement(2019..2672)
                     /locus_tag="WJL87_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ7799682.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYSDIALIIVAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2823..3113
                     /gene="gatC"
                     /locus_tag="WJL87_001258"
     CDS             2823..3113
                     /gene="gatC"
                     /locus_tag="WJL87_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ7799683.1"
                     /translation="MALTLADVDKIARLSRLHLTAAEKEKSLQELNDIFAMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAVAPEVRNRLYIVPQVIEE"
     gene            3176..4621
                     /gene="gatA"
                     /locus_tag="WJL87_001259"
     CDS             3176..4621
                     /gene="gatA"
                     /locus_tag="WJL87_001259"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ7799684.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNPEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAITGFDPKDSTSLEREKEDYTRDLNQPLKGVKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEAGTNLSRYDGVRYGHRAAQFA
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4618..5550
                     /locus_tag="WJL87_001260"
     CDS             4618..5550
                     /locus_tag="WJL87_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HEZ7799685.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLTDIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEARGISDGIQTEKPLRRTA"
     gene            5593..7023
                     /gene="gatB"
                     /locus_tag="WJL87_001261"
     CDS             5593..7023
                     /gene="gatB"
                     /locus_tag="WJL87_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEZ7799686.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAYASVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPELR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGSKVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ASGQGKPTANWMNGELAAALNKAGLELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPESTIAEIIEKHGLQQMTDTGAVEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7115..8125
                     /locus_tag="WJL87_001262"
     CDS             7115..8125
                     /locus_tag="WJL87_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ7799687.1"
                     /translation="MNHTVTLPDQTTFAAGDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQIGGHSEQALSEAEKAQGKILMCCTTAQSDISLNIPGYKADALPVRTLP
                     ARIESMVFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANSPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQDSGRAVHFYWGARHQDDLYALEEAQGLACRLKNACFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(8254..8886)
                     /gene="pdxH"
                     /locus_tag="WJL87_001263"
     CDS             complement(8254..8886)
                     /gene="pdxH"
                     /locus_tag="WJL87_001263"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ7799688.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
BASE COUNT         2278 a         2743 c         2170 g         1731 t
ORIGIN      
        1 tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctatattttt gggaatctgt
       61 tttaccccaa tatataaagc accatattaa ggcggagttt cttccccact ttgaaccgaa
      121 cccggaaaag acaccgccca agccaatcct gatgctgccc cggcagccaa ccatcaagga
      181 aaccctaatg aactttgctt tatccgtcat cacatttacc ctcgcctctt tcctgcccgt
      241 cccgcccgcc gaagccgccg tctttacttg gaaggacggc ggcggcaaca gctattcgga
      301 tgtgccgaaa cagcttcatc ccgaccagag ccaaatcctc aacctgcgga cgcgccaaac
      361 caaaccggcg gtcaaacccg cccaagccga cgcagggaag cgcacagacg gcgcggcaca
      421 ggaaaacaat cccgacactg ccgagaaaaa ccggcagctt gaggaagaaa agaaaagaat
      481 tgccgaaacc gaacggcaga acaaagaaga aaactgccgg atttcaaaaa tgaacctgaa
      541 agcggtggga aattcaaatg caaaaaacaa ggatgatttg attcggaaat acaataacgc
      601 cgtaaacaaa tactgccgtt aatcggctct agcgcaaacc ccatgccgtc tgaaacggca
      661 cggggtttgt ttatttctgc cagtaggttt tgacgttgac gaactcgtac agcccgaatt
      721 cggacaattc gcgcccgtaa ccggaatctt tgactccgcc gaaaggcagg cgcaaatcgc
      781 tgctggtatg gcggttgata aacaccgatc ccgcctgtat tttttcggca aaccgccaag
      841 cgcgttcggt atcggcggta taaatgcagg cacctagccc gaacggggaa tcattggcaa
      901 gggcgatggc atgttcttcg ttttcggcgc gcaaaatcag ggcggccggc ccgaatactt
      961 cttctctcca gacgcggcag gcaggattta ccctgtctaa aaccgtcgcg ggataaaacc
     1021 agcctcgccc ttgtgggatt tttccgccgg tcaggcatac cgcgccgttt gaaacggcat
     1081 cttcaacctg cccgtgaacc ctgtcccgca aatcttcgcg gtgcagcggt gcaagcgtag
     1141 tatcgggatg tttggggtcg cccattttca atttggcgca ttcggcaaga aacagcgtga
     1201 taaaacgatc ggctgcggct tcggttacga tgatgcgttt ggcggcgttg cacgattgcc
     1261 ccgcatcgcg gaaacgggaa taacaggctt ctgcggcggc gcgttccaaa tcggcatcgg
     1321 gcatcacgat aaaggcgttg ctgccgccga gttccaagac ggttttcttg aggtttgcgc
     1381 ccgcatgtgc cgcaaggatg cgccccgtat gcgtcgagcc ggtaaacgcc atcgcatcgg
     1441 tatcttcaac cgccttgagc gtgcccgcct catccaacca cgcgcctgcc aaaggaatgc
     1501 cgtctgaaac caaatcaaac aacgcctgac tgacgcgtgc cacgctgggc gcgggtttga
     1561 cggcgcacgc gttgcccgcg cacatagcgg gaacggcgaa acgcaatacc tgccagacgg
     1621 gatagttcca aggcatgacg gcaaacacca cgcccaaagg ctcaaacctt acctgactca
     1681 aactcgcctg cgtcgcgatg gttttgtggg cgagcagttc gggagcgagg cgggcgtaat
     1741 agcgtatcag ttcgatagac ttgccgattt ccgcacggca ttcgtgcagg cagcgtccga
     1801 cttcttcgca caccatttcc gcaaaacgct ctttctccgc ctccaaacgg tcggcaaatt
     1861 tttgcagacg cgcggcacgt tcggttacgc ccagttgcgc gaacgccccg ccgcgcattt
     1921 tcaaatccgc cagccgccgt tcaaactccg cataatcttg agctggacgg cggtaaagcg
     1981 tttcgcccgt aaatacattg acactgtgaa acatcgaatc aacctgccag ttgcgggaat
     2041 atcgttttca gtcccgacac aataatctcc accgataccg ccgccagcat catacccatg
     2101 atacggttta aaatcgtcag ccccgtcgcg cccagcaggc ggctgacctt ccctgcaaca
     2161 attaaaatgg cataacaaat cgcactgacc accaaaccgg ccacgataat cagcgcgatg
     2221 tcgctgtacg ttttggctgc cgaagcgtaa ataatcacgg tcgaaatacc gcccgggccg
     2281 atggtgatcg gtatggcgat gggcacgacg gcaatcgctc cggcattgcg ggcggggcgc
     2341 gcctgccccg tttccggctg cgcgccgaga ttctgcttgg cgggattgtc gttgccgttc
     2401 atcatcgaaa tggcgatcag cagcaccaaa atcccgccgc cgacctgaaa cgaaccgacg
     2461 ctgatgccca aaaccttcag cagcgtaccg ccgatcagcg caaataccgc aatcacggca
     2521 aacacggcaa cggcggccgt ccgcgcgacc ttcctgcgct ccttcgtgct gtgcccgttg
     2581 gtcaggtcaa ggtaaagcga caacgcgcta aacggattaa tcagcaccaa aaaagccaca
     2641 atcagcttgc cgatttccat gcccaatccc attatttccc cctccttcaa acccgtgcgg
     2701 caggcatccg atgctgcaaa ttgccgccgc aacggatttt tccgttataa ttaaaaattc
     2761 aagcaatacg ccccatcata cccgaacgac ggtatcttta ccatcagaca aggatgcttt
     2821 tcatggcact gacacttgcc gacgtagaca aaatcgcccg actctcccga ctgcacctga
     2881 ctgcggcaga aaaagaaaaa tcgcttcaag aattaaacga cattttcgct atggtcgaac
     2941 agatgcaaac catcaacaca gacggcatcg aaccgatggc tcatccgcac gaggccgccc
     3001 tgcgcctgcg cgaagacgaa gtaaccgaaa ccgaccgcgc cgccgaatat caggcggttg
     3061 ctccggaagt acgcaaccgt ctgtatatcg taccgcaagt tatcgaagaa taatccgaat
     3121 atgcttcaga cggcatcagc aataccgccc gaagcccttt aaggatggaa gatttatgac
     3181 ccaatacaca ttgaaacagg caagcgtcct gttgcagtcc aaacagattt ccgccgtcga
     3241 actggcaagc gcataccttg ccgccatcgc cgaaaaaaat cccgccctca acggctacat
     3301 caccatcgac caagataaaa cccttgcaga agcccgtgcc gccgacgaac gtatcgcgca
     3361 gggcaacgcc tccgcgctta ccggcgtacc cgtcgcctac aaggatattt tctgccaaac
     3421 cggctggcgc agcgcgtgcg cttccaaaat gctcgacaac ttcatctccc cttacaccgc
     3481 caccgtcgtc caaaacctgc tcgacgaagg catggtaacg ctcggccgca ccaatatgga
     3541 tgagttcgct atgggttcga ccaatgaaaa ctcattctac ggcgcggcca aaaatccgtg
     3601 gaatccggaa cacgtccccg gcggttcgtc aggcggttcc gccgccgtcg ttgccgcgcg
     3661 ccttgcccct gccgcgctcg gttcggatac cggcggctct atccgccagc ctgcatcgca
     3721 ctgcggcatt accggcatca aacctaccta cggcacggtt tcccgcttcg gtatggtcgc
     3781 ctacgcctcc agcttcgacc aggccggccc gatggcgcaa actgccgaag actgcgcgat
     3841 tctgttgaat gcaataacag gtttcgaccc caaagactcc accagtctcg aacgtgaaaa
     3901 agaagactac acccgcgatt tgaaccaacc gctcaaaggc gtgaaaatcg gtctgcccaa
     3961 agaatatttc ggcgaaggca acagcgccga tgttctgacg gcattgcaaa acaccattga
     4021 tttgctgaaa gcccaaggcg cggaattgat tgaagtttcc ctgccgcaaa ccaagctgtc
     4081 catccccgcc tactacgtcc tcgcctccgc agaagccggc accaaccttt cacgttacga
     4141 cggcgtacgt tacggacacc gtgccgccca attcgccgat ttggaagaaa tgtacggcaa
     4201 aacccgcgcc gaaggtttcg gcagcgaagt caaacgccgc atcatgatcg gcacttatgt
     4261 actgtcgcac ggctactacg atgcctacta tctcaaagcc caaaaactgc gccgcctcgt
     4321 tgccgatgat tttcagacgg catttgcacg gtgcgacctc atcctcgcgc cgaccgcacc
     4381 cactgcagcc ccaaaaatag gagcggatgc ttcgccggtt gaaacctact tgagcgatat
     4441 ctacaccatc gccgtcaacc tcgccggact gcccgcattg accctgcccg caggcttcag
     4501 cggcggcgga ctgcccgtcg gcgttcagct tgtcggcaac tacttcgccg aagccaaaat
     4561 cctcggtgcg gcgcatcaaa tccaactcaa cagcgattgg cacggcaaac gacccgaatg
     4621 aagcagaacc gcacctttac cttccccgat tttcgcaccg tttacagcta tgcgccttta
     4681 tatcggctgc aacatttaaa atacacattg cgaaaatttt tcggaaaaaa agaaatttac
     4741 gccttcgagc agtttgtcaa cgcctcccct atccgtcagg ggctgttcct ccactgcccg
     4801 caaaatgcct atccgctgct gcgcgaattt gttgacaggc gttttaactg caaacgccgt
     4861 ttagatgcga tgacggcaga ttttctcatg gcggaaaaac tgttcggcac agacatcctg
     4921 caccaaatgg aagactaccg cttccatttg gtcttggcgc acctttcaga cggcatcagc
     4981 ttgtggctca accgcaacga caactgcgtc gaagaaggcg cgtggtcttt atctttgcgc
     5041 gacgaagcag gcaaccggct gtatatggcg actttcgcct ttgtcggcac acacctgctg
     5101 acagcctccg tacaagggcc tgcgggtgaa gaagccaaag acactgtccg ccgcataacc
     5161 aaacaacttc acggcttgcg tccccaacaa ctgatggtaa ccgcactgca atatttcgcc
     5221 gccgtactcg gcttggacgg cgcaatgggc attgcacaaa aacatcaggt caaactgcgc
     5281 tggaaactta aaaagcgcgt caaaatgaat tacgacgcat tctggcagga atacggcgca
     5341 agtttggaac gggacggcta ctggcatctc ccccaaaccc ccgcccgcaa agaccttacc
     5401 gacatcgaaa gcaaaaaacg ctcgatgtac cgcaagcgtt atgaaatgct ggacaacatg
     5461 gttgcagaga tgaaggacgg tctgaaaaca gaagcacgcg gcatttcaga cggcatccaa
     5521 acggaaaaac cgctccgccg gacagcctga cgcgaagact atcgaattga tattttagag
     5581 aaagaagctc ttatgacctg ggaaaccgta atcggcttgg aaatccacgt ccaattgaac
     5641 accaaatcca aaatcttcag cggcgcatcg accgcattcg gcgcagaacc caacgcgtac
     5701 gccagcgtag tggaatgcgc gctgccgggc gtactgccgg taatgaaccg agaagtcgtt
     5761 gaaaaagcca tcaaattagg cttggctttg gatgcgaaaa tcaaccggaa aaacgtgttc
     5821 gaccgtaaaa actacttcta tcccgactta ccaaaaggct atcaaatcag ccagttggat
     5881 ttgccgattg tcgaacacgg caaattggaa atcgtagtcg gcgacgatgt gaaaaccatc
     5941 aacgtaaccc gcgcccatat ggaagaagac gcgggcaagt ccgtgcatga aggcttgaac
     6001 ggcgcgaccg gtatcgacct gaatcgcgcc ggcacgcctt tattggaagt cgtatccgaa
     6061 ccggaattgc gctccgccgc cgaagccgtt gcctacgcca aagccttgca cagcttggta
     6121 acctggctgg acatttgcga cggcaatatg gcggaaggtt cgttccgcgt cgatgccaac
     6181 gtatccgttc gcccgaaagg tcaagcggaa ttcggcacgc gccgcgagat taaaaacctc
     6241 aattccttcc gtttcttgga gcaggcgatt aattacgaag cggaagcgca aatcgagatt
     6301 ttggaagacg gcagcaaagt gcagcaggcg acgatgctgt tcgacccaga aaaaggcgaa
     6361 acccgcgtaa tgcgcctgaa agaagacgcg caagactacc gctacttccc cgacccggat
     6421 ttgttgcccg tcatcatttc agacgctcaa atgcaaaaag ccaaagcaga aatgcccgag
     6481 ctgccgaaag aaatggcggc acgtttcgtg gcagattacg gcgtgtccga atacgacgcg
     6541 cgcctgctca ccgcaagccg cgcacaggcc gcctattttg aagaagccgc caaagcaagc
     6601 gggcaaggca agccgactgc caactggatg aacggcgaac tcgccgccgc gctgaacaaa
     6661 gccggcctgg aacttgccga cagcccgatt accgccccgc gcctcgccgc gctggtgggc
     6721 aaaatcgccg acggcacatt aagcagcaag ttagcgaaaa aagcctttga agccatgtgg
     6781 gcagaacccg aatccaccat tgccgaaatc attgaaaaac acggcttgca gcaaatgacc
     6841 gataccggcg cagttgaagc gatggtggac gaagtgctgg caaacaacgc caaagccgtg
     6901 gaacagttta aatccggcaa cgaaaaagcc ctgaatgcga ttgtgggaca agtgatgaaa
     6961 gccagcaaag gcaaagccaa ccccgcgcaa gttcaagaac tgattaaagc caaacttgcc
     7021 tgataagcaa gatgccgtct gaaacccaga tccgacgttc agacggcgtt ttcacagtct
     7081 gaccacatta aaacagcata aaaaacaata ttccatgaac cacaccgtta ccctgcccga
     7141 ccaaaccacc tttgccgccg gcgacggtga aaccgttttg gccgctgccg cccgtcaaaa
     7201 cctcaatctg ccccattcct gcaaaagcgg tgtctgcggg caatgcaaag ccgaactggt
     7261 cagcggcgat attcaaatag gcggacactc ggaacaggct ttatccgaag cagaaaaagc
     7321 gcaaggcaag attttgatgt gctgcaccac tgcgcaaagc gacatcagcc tcaacatccc
     7381 cggctacaaa gccgatgccc tacccgtccg caccctgccc gcacgcatcg aaagtatggt
     7441 tttcaaacac gatgtcgccc tcttgaaact tgccctgccc aaagcccctc cgtttgcctt
     7501 ctatgctggg caatacatcg atttactgct gccgggcaac gtcagccgca gctactccat
     7561 cgccaattcg cccgaccaag aaggcatttt ggaactacac atccgcaggc gcgaaaacgg
     7621 tgtctgctcg gaaatgattt tcggcagcga acccaaagtc aaagaaaaag gcatcgtccg
     7681 cgttaaaggc ccgctcggtt cgtttaccct gcaggaagac agcggcaaac ccgtcatcct
     7741 gctggcaacc ggcacaggct acgcccccat ccgcagcatc ctgctcgacc ttatccgcca
     7801 agacagcggc cgcgccgtcc atttctactg gggcgcgcgt catcaagatg atttgtatgc
     7861 cctcgaagaa gcacaagggt tggcatgccg tctgaaaaac gcctgcttca cccccgtatt
     7921 gtcccgcccc ggagagggct ggcagggaag aaaagggcac gtacaagaca tcgcggcaca
     7981 agaccacccc gacctgtcgg aatacgaagt atttgcctgc ggctctccgg ccatgaccga
     8041 acaagcaaaa aatctgtttg tgcaacagca taagctgccg gaaaacttgt ttttctccga
     8101 cgcattcacg ccgtccgcat cataattccc cggtataaaa aagattcagg ctttccgttc
     8161 ggaacacaaa aaacttcccg tccgtatttt ccccataaaa aatgccgtct gaagcccgat
     8221 tccggttttc agacggcata tgttttttcc tgttcaaggc gacagccgct cgcgtatcca
     8281 gccaccatcc agcaaacggt attggatgcg gtcgtgcagc ctgctcggcc tgccctgcca
     8341 aaactcaatc aaatcgggaa tcacaatata gccgccccaa tgcggcggac gcggcacgtg
     8401 caaaggatgt ttgagtccga ccgccgccgc ctttgccacc aataccgcct tgttcggaat
     8461 cacctcgctc tgcgcacttg cccacgcacc caaacggctc tgatacgggc ggctctcaaa
     8521 atattcgtcc gacaacttct ccgccagcct ttcaacacgc ccttccacgc gcacctgacg
     8581 ctccagctcc ggccaaaaaa acgtcatcgc cgcaaatgga tgagcatcca gcgaacgccc
     8641 cttgcggctg tgataattcg taaagaaaac aaaaccttca gaattaactt ccttcagcag
     8701 caccatacgg ctgttaggcc tgccgcgtcc gtcgaccgcc gccacattga ccgccgtcgg
     8761 ctcgttgacc tgtgcgcgta ccgcctcgtc caaccaccgc tcgaactgct cgatcggatt
     8821 gtcggcgcaa tcgccttccg acaattcccg tttgctgtaa tcttcccgaa tattgtgcaa
     8881 atccatttac cgctcctctt aatagtggat taacaaaaac ca
//
LOCUS       DAWKST010000079         8824 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.079, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000079 DAWKST010000000
VERSION     DAWKST010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8824
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.079"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            179..1354
                     /locus_tag="WJL87_001264"
     CDS             179..1354
                     /locus_tag="WJL87_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ7799689.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(1565..2113)
                     /locus_tag="WJL87_001265"
     CDS             complement(1565..2113)
                     /locus_tag="WJL87_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ7799690.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNTESK"
     gene            complement(2116..3612)
                     /gene="ccoG"
                     /locus_tag="WJL87_001266"
     CDS             complement(2116..3612)
                     /gene="ccoG"
                     /locus_tag="WJL87_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ7799691.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            4140..6119
                     /gene="tkt"
                     /locus_tag="WJL87_001267"
     CDS             4140..6119
                     /gene="tkt"
                     /locus_tag="WJL87_001267"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ7799692.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEKGAKLEAEWDKLFAQYQAKYPAEAAEFVRRMDKKLPENF
                     DGYVQTALKEVCAKAETVATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIINGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGGYVISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDTAYQAAVLPEGLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKEFGFTVDNVVDTVKSVL"
     gene            6267..7655
                     /gene="fumC"
                     /locus_tag="WJL87_001268"
     CDS             6267..7655
                     /gene="fumC"
                     /locus_tag="WJL87_001268"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ7799693.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYKLALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAVGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(7720..8643)
                     /locus_tag="WJL87_001269"
     CDS             complement(7720..8643)
                     /locus_tag="WJL87_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ7799694.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKWRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAKPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYVGALVVVGGAVTAAVGDRLFKRR"
BASE COUNT         2231 a         2537 c         2160 g         1896 t
ORIGIN      
        1 cactataata ttttctctcc cgattgaaac aggcgtaaca gaatatgccc gaagctccgg
       61 ctgctttctt gtttaccgcc gcgatattta gagtataata ccaaatttga gcaatagttc
      121 taaaacagtt agaaccattt ttcatgagcc tgactgattc gtacactcgg agaaactgat
      181 gcagaatatt tttgaccctt tggttattcg tggaaaatcc cttaccccca tcgtgcaagg
      241 cggtatgggg gtcggtgttt ccgcatcggg tttatccagc gcggtggcgc gtgaaaacgg
      301 tatcggaacg attgccagtg tggatttgcg ccaccttcac gaagacctac tcgccgaatc
      361 acaaatcaat ccgagtgaag agaaatatac atctttgaac tgtaccgcat tagacaggga
      421 aatccaaaaa gccaaaagcg cttcagaggg aaaaggactg attgcggtca acgtgatgaa
      481 ggcggtcaaa gaccacgccg cgtatgtccg ccaggcttgc gaatcagggg cggatgcggt
      541 tgtaatgggt gccggcctgc ctttagacct gccggaaatg accgagggct atcataaaga
      601 tgtcgcgctg ctgccgattc tgtccgaatc gcgcggtatt aatatcgtct tgaaacgttg
      661 gatgaaaaaa ggcatattgc ccgatgcgat tgtagtcgaa catcctgccc acgcggccgg
      721 acatttgggt gcatcaaccg ttgaaggcgt aaacgatgcc aagttcgact tcaaacgcgt
      781 gattgaggaa acgtttgaag ttttcaaaag tttagggctg gaaagcgaaa aaatcccgct
      841 tattcttgcg ggaggcatgg caaattttga aaaagtcaaa accgccctaa agaactgggg
      901 agcatccgcc gttcaaatcg gtacggcttt tgccgttacc gaagaaggag atgcacacct
      961 taacttcaaa aaaacgctcg ccggtgcgga aactgaaaaa gtagtcgaat ttatgtctgt
     1021 tgccggtttg ccggcgcgcg gtgtccgcac caaattccta gacagctaca tcaagcgtga
     1081 aagcaaactt cagacaaacg ccaaagccga cccgcgccgc tgtacccaag gtttaaactg
     1141 cctaaccagt tgcggtctgc gcgacgggct ttccaaagca ggacagttct gtattgatat
     1201 ccagcttgcc gccgcattcc gtggagaagt agataaaggc ctgttcttca gaggtaaaga
     1261 cccgctgccc ttcggcaatg ccatccgcac cgtccgcgag acgatacaat atctgctgac
     1321 ggggagcgaa cctgttgcaa cgctcggacg ctgacatcag gtaggatttg atttgcaaac
     1381 aaaatgccgt ctgaagggct ttcagacggc gtttttcagg ctgcgttccg gaatagtgtt
     1441 aaaaaataaa cgggatgaga tacatttatt ttcgtccgac aaatcaaacc atcgcgccga
     1501 atgatcaaat aatgcctgca cggcattaca tctggcaaag caatgcaatg aaaacacggc
     1561 ttttttattt gctttcagta ttattgaaaa gcttgtccat cggggtcaaa tcgaccgcat
     1621 tgccttggct ggtaatccat ttgttctcga cgcgccacac gcctgccgcg tcctccacgc
     1681 gcacatacca gtggttggtc ggcgaaaggg ttttgaacac cgcctcatat tccgccctgc
     1741 cgttctgcgc gctgccgacg ggcttgaggg cgacggtttg atcgtccgcc ttgcgggtcg
     1801 ggtgcatcag cagcaggttc aaaggctgtt tgccgtcaaa ctcgccgccg acaaacactt
     1861 ttgccgcatt catatcgggg gaaatgagaa cctgcacccc gatatgccgt ctgacggctt
     1921 cttcatcccg atgaagctgg atgtcgatat gtttgccgtc tttataataa tcgtccgtaa
     1981 ccaaatctgt cgcgtgctgc tgcgcgacaa aaaacatagc gacgctggcg atgacgacaa
     2041 aaatcggccc cgccatcaag atccacggcc agacgtgttt gtaccaaggt ttgattggag
     2101 tgttttgaga cacggttatt ctccgataaa ggttgcatct tcttccaaga cgaccggctt
     2161 gccgtcgggc gcgccgcttt cgcggtattg gaaggtaaat tcgatagggt ggctgccttt
     2221 gtccgcgtat tccggaatgg tggacacttg gacgggaagg gttaccgttt cgcgcggggc
     2281 aaccttgata ccgccttcgg gcagcccggt cagggcgatt tcgtcaaagc ctttgacact
     2341 tgcggtaatg agctgttctt tttcactttt gttgatgata cgcaggctgt atgcgttttc
     2401 cagccagcct ttggcgtttt cgcgcaccag tacgccacgg tctttcaaaa tatcgacctc
     2461 gaccattttg cgcgtggaca aaccggccag gaaggcaatg ataactaacg ccaacaccgc
     2521 gccgtaacct gccacgcgcg gtctgagcag ccgtttttta atgtcttttt cagaatattc
     2581 gtgttccagc gcgctttcgg tcgtataacg gattaatccg cgcggatagc ccattttgtc
     2641 cataatctca tcgcacgcgt cgatacaggc ggcgcagccg atacattggt attgcagacc
     2701 gttgcggatg tcgatgccga cggggcagac ttggacgcac atcgcacagt tgatgcagtc
     2761 gcccaaaccc gcctcttcct tattgaccgt tttcttgcgc gcgccgcgcg gttcgccgcg
     2821 ttccgcgtca taagaaacaa tcagcgtgtc cttgtcgaac atcgcgcttt ggaaacgtgc
     2881 atacggacac atatgcaggc atactttttc acgcataatg tgggcgaaga agaaggtcat
     2941 aaagccataa aacgctgcgg caaacatcgc gccgccacct gctgctccag tgaataaatc
     3001 gggaacgaac tggcggatag ggacaaacca gcctgcaaac gtgatgcccg tccacgcgca
     3061 gacaaggaaa atcagcaggt atttggtggc tttgatgcgg attttagtga aattccacgg
     3121 cgatttttcc agtttcagcc gtttgtttct atcgccttcg accaggttgt caatccacag
     3181 cataatttcg gtgtaaaccg tttgcgggca ggaatagccg caccacagtc gccctgcaat
     3241 cgtcgtccac caaaacagcc cgaaggcgca aatcatcagc agcaaggcaa ggtaaatcaa
     3301 atcgcccacc cccaacgaca atccgaaaat gaagaaatgc cgttcgggga tattgaaaac
     3361 gacggcctgc ctgccgctcc agttgaacca cggaatgacg taaaacacaa actgcgtcgc
     3421 caatacggcg gcgatacgca gtttggcgaa ccgtccttcc gcctttttgg gatggatgcg
     3481 ttcgccttcg ggatggattt gaatcacgct ggctcgcgga tcgaatgttt tttttgcttt
     3541 cggcgcggct tttgtttgtt cggacgtgcc gattccggat gccggactgc cggcttggtt
     3601 ttccgtggtc attctgcatt ccttagattt ttgattgatg gtttgcccgt taccgccgcc
     3661 gtttgctttt cagacgtcat ttttcttgtt ttttaaggcg ttgtgtttca agttttgaga
     3721 aaatccgttt ttcccaaaat atatttccgc tattgtacaa ctttatgcgc cgtccggatg
     3781 tatggggcgg atacatttcc catccgcatc aaaacgcctg gattttacct taccgcccga
     3841 acaaaatccg aatacggtta aaaaaaaaga ctaaaaaaac cgacaccccc atatcggcag
     3901 aaccgacggc gcaagctcat aaacaaacgc tatcgacaat ccggcacaca atctataact
     3961 ttttatttca aaaggaataa tggcaggctt cgcccgcaaa tcgaaaatcc ttccccgcct
     4021 gtcccctgcc gccgccttcc cacgcgtccg cccttttctt gaaagcataa gcgaatcggg
     4081 cgataatcaa cgctttccga ttatccactt atctgaaaca ccagcaagga aaatacaaaa
     4141 tgtctcaact ggcaaacgca atccgcttcc tctcggccga tgccgttcaa aaagccaatt
     4201 ccggccaccc cggcgcgcct atgggtatgg cggaaatggc ggaaacattg tggacgaaat
     4261 tcctcaatca caaccccgcc aaccccaaat tctacaaccg cgaccgcttc gtcctctcca
     4321 acggccacgc gtctatgctg ttgtacagcc tgctgcacct gaccggctac aacctaagca
     4381 ttgaagactt gaaaaacttc cgccaactgc acagcaaaac ccccggccat cccgaatacg
     4441 gctacaccga cggcgtggaa accacgaccg gcccgttggg gcaagggatt gccaacgcgg
     4501 tgggtatggc attggcagaa aaaatccttg ccgccgaatt taataaagac ggtttgaaca
     4561 tcgtcgatca ttacacctac gtctttatgg gcgacggctg tctgatggaa ggcgtatcgc
     4621 acgaagcctg ttcgctcgcc ggcaccttgg gcttgggcaa actgattgtt ttatatgatg
     4681 acaacaatat ttccattgat ggtaaagtgg acggctggtt taccgaaaac atcccgcaac
     4741 gctttgaaag ctacggctgg cacgtcgttc ctaacgtaaa tggtcatgac accgccgcca
     4801 ttcaagccgc catcgaagcc gcacgtgccg aaaccggcaa accgtccatc atctgctgta
     4861 aaaccttaat cggcaaaggc agtgccaata aagaaggcag ccacaaaacc cacggcgcac
     4921 ctttgggtgc ggacgaaatc gaagccacgc gcaaacattt gggctggact taccctgcct
     4981 ttgaaatccc gcaagaaatt tacgatgcgt ggaatgccaa agaaaaaggc gcgaaactgg
     5041 aagccgaatg ggacaaactg ttcgcgcaat accaagccaa atatcctgcc gaagccgcag
     5101 aatttgtgcg ccgtatggat aaaaaactgc cggaaaactt tgacgggtac gttcagacgg
     5161 cattgaaaga agtgtgcgcc aaagccgaaa ccgtcgccac ccgcaaagcc agccaaaaca
     5221 gcatcgaaat cttggcaaaa gagctgcccg aactggtggg cggctctgcc gacctgaccc
     5281 cgtccaatct gaccgactgg tcaaacagcg tctccgttac ccgcgacaaa ggcggcaact
     5341 acatccacta cggcgtgcgc gagttcggca tgggtgcgat tataaacggc ttggtattgc
     5401 acggcggcgt gaaacccttt ggcgcgacct tcctgatgtt cagcgaatac gagcgcaacg
     5461 ccctgcgtat ggcggcactg atgaaaatca accccgtgtt cgtgttcacc cacgattcca
     5521 tcggtttggg cgaagacggc ccaacccacc agccgattga gcaaaccgcc accctgcgcc
     5581 tgattccgaa tatggacgta tggcggccgt gcgacaccgc cgaatccttg gtcgcttggg
     5641 cagaagccgt caaagccgcc gatcacccgt cctgcctgat tttcagccgt caaaacctga
     5701 aattccaagc gcgcagcgaa caacaactga acgacatcaa acgcggcggc tacgtcatca
     5761 gcgaagccca aggcaacgcc caagccgtca tcattgccac cggttccgaa gtcgagctgg
     5821 ctttggaagc gcaaaaagca ctcgccgcgc aaaacatcgc cgtgcgcgtc gtttccatgc
     5881 cgtccaccaa cgtattcgac cgccaagaca ccgcctatca agccgccgtc ctgcccgaag
     5941 gcctgccgcg cattgccgta gaagccggac acgccgacgg ctggtacaaa tacgtcggcc
     6001 tgaacggcgc agtcgtcggc atcaaccgct tcggcgaatc cgcccctgcc gatctgctgt
     6061 tcaaagaatt cggctttacc gtggacaatg tggtcgatac agtgaaatca gtgctgtaac
     6121 cccacaccta aacaaatgcc gtctgaaacc aattagggct tcagacggca tttgtttgcc
     6181 ctctctcctt gatacttcct cattcctcca atctccatta taatatttgc gaatcactct
     6241 tattcacatt tcaaaaggag aaacgtatga acacccgcac cgaacacgac accatgggca
     6301 atgtcgaagt cccgtccgaa gcctattggg gcgcgcagac ccagcgcagc cgcaacaatt
     6361 tcaaaatcgg cggcgaaacc ctgccgcagc cgctgattta tgctttggcg ctggtgaaaa
     6421 aagccgctgc cgcgaccaat gtttccctcg gcaggattaa gccggaacag gcggatttga
     6481 ttacgcaggc ggcggatgat gtgttgaacg gcaggcttga cggacagttt cctttagtgg
     6541 tttggcagac cggctccggc acgcagtcca atatgaacat gaacgaagtg ctggcgaacc
     6601 gcgccaacga aatcgccggt acgggtttgg cggcgtatca gcccgtccat cccaacgacc
     6661 atgtgaacca cgcgcaatcg accaacgacg cattcccgac cgccatccac gttgccgccg
     6721 cgattgaaat caaccgccac ctcatccccg ccgtaaaagc cctgcgcgac accttggaca
     6781 aaaaagccca agccttcgcc cccatcgtca aaatcggccg cacccacttg caggacgcga
     6841 cgccgctgac tttaggccaa gaattttccg gctacgtttc ccagctcgac cacggcttag
     6901 gccgtctgaa tgacgcgctt aaagacttgt ataaacttgc tttgggcggt acggcggtcg
     6961 gcacgggttt gaacagccat cccgaatacg ccgaaaaagc cgccgccaaa ctcgccgaat
     7021 tgtccggctt gccgtttgtc agcgcgccga acaaatttga agccttgggc ggacgcgatg
     7081 ccgccgttgc cgcttcgggc gcattgaaaa cgctggcggc aagcctgaat aaaatcgcca
     7141 acgacatccg ctggctggca agcggcccgc gctgcggttt gggcgaaatc aaaatccccg
     7201 aaaacgagcc gggttcgtcc atcatgccgg gcaaagtcaa cccgacccaa tgcgaagcga
     7261 tgaccatggt gtgctgccaa gtgttcggca acgacgttac catcggcatg gcgggcgcgt
     7321 cgggcaattt cgagctgaac gtctatatgc ccgttatcgc ctacaacctc ttgcaatcca
     7381 tccgcctgtt gggcgacgcg tgcaacagct tcaacgaaca ctgcgccgtc ggcatcgaac
     7441 ccgtaccgga aaaaatcgac tatttcctgc accattccct gatgctggtt actgcgttaa
     7501 accgtaaaat cggctacgaa aacgccgcca aagtcgccaa aaccgcctac aaaaacaaca
     7561 aatcgttgcg cgaaaccgcc gttgagttgg gcttgctgac gggcgaagaa tttgacgaac
     7621 tggtcgttcc tgccgatatg gttcatccgc gctaatcttt ccctcaaata aaatgccgtc
     7681 tgaaacctcg ttcagacggc attttccgtt gcctgcgaac tagcggcgtt tgaacagcct
     7741 gtcccccacc gccgccgtaa ccgcaccccc gaccacgacc agtgcgccaa cataacccaa
     7801 accgttcata tccggcgcgg caaaagtatc aggcatcaca taatgcccga gcaaagaaaa
     7861 tattacggta aacacgggga gcaaggttgt taccgcgctg actttggaag cctcccaatg
     7921 tttcaacgcc tcgccgaacg agccgtaacc gattaacgta ttcaagcagc aatacgcaaa
     7981 acaaacccac gccaacgtac cgtccaaact tccgatgtgt gccggtttgg caaacggcag
     8041 gaacacggcg gcacttgccg cataaatcaa cagcagaatc tgttgcggtc cgaattgcgc
     8101 cgacagcagc ttttgcgcca cggcataaca cacccatgcc atactgcctg ccgcacacag
     8161 caacacgccc ttcgcatacg cgcccaaacc cgacaactcg ccgaatttat cgttaaaaaa
     8221 cataagcaaa ccggcaagca gcaaaaccaa gccgattttc tgagcggcag tcatccggtc
     8281 tttaaacacc aacacaccga caacaatcat cgtaaacggc gaaatctgcc acaaaacctg
     8341 cgtcgtggtc ggcgaaatat aatgcagccc ttgggcaatc agcacaaagt ttgccgaaat
     8401 gcccgccacg ccgagcagca gcagcctgaa tgagcaccaa gaaaaatccc gccacttcgg
     8461 cagccgcccg cccaatgcca gcaaaacaaa caataccgcc gccgccacgg taaaacgcac
     8521 ccacaccagc gtcggcgcat cgacaaactt caatacctgc cgcacggcaa tcggcagcgt
     8581 tccccacgtc atcgccgcca aaagtgccaa cgcgaagcct aggagcggcc tttggttttc
     8641 catcctgatt ttcctatttt taaacaaccg tattgccgga cgatgccggt ttgccgcatc
     8701 gggcaatgat ggttcaagcg tttgacgttt gattccaacc ctttgatttc aaacaaaccg
     8761 gctgaagctc ggctattgct tcgcgctatt tgaaaacacc gcctgaattt taaaatatag
     8821 tgga
//
LOCUS       DAWKST010000080         8608 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.080, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000080 DAWKST010000000
VERSION     DAWKST010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.080"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            122..1507
                     /locus_tag="WJL87_001270"
     CDS             122..1507
                     /locus_tag="WJL87_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ7799695.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            1527..2045
                     /locus_tag="WJL87_001271"
     CDS             1527..2045
                     /locus_tag="WJL87_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ7799696.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(2297..3214)
                     /gene="thrB"
                     /locus_tag="WJL87_001272"
     CDS             complement(2297..3214)
                     /gene="thrB"
                     /locus_tag="WJL87_001272"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ7799697.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLVAADFPLEMENPRYDAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(3251..3979)
                     /gene="ubiG"
                     /locus_tag="WJL87_001273"
     CDS             complement(3251..3979)
                     /gene="ubiG"
                     /locus_tag="WJL87_001273"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ7799698.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACANLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            4131..5372
                     /locus_tag="WJL87_001274"
     CDS             4131..5372
                     /locus_tag="WJL87_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ7799699.1"
                     /translation="MPTKTPSLFGCAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGIGNALGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKMPVLFDTQAPAGTGYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWMGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            5430..6485
                     /locus_tag="WJL87_001275"
     CDS             5430..6485
                     /locus_tag="WJL87_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7799700.1"
                     /translation="MELKIDIATNNFKHGGGTERYTLDLVKGLNRQNITPAVYATKFD
                     HGIPEYAMIEPHLVDQHRTLKKLRSFLFSSRLAQTRKNSAAKLIACHHADYADLLICG
                     GTHLGYLHHMAQKPNLLDRLAIRRNRSNYATAKLIMAHSHMMRRELVGLYGVPPERIQ
                     VAPPPADTERFFPQPGETADLRAKYGFADHETVFLFPSTGHTRKGLELLADFFEHTGL
                     PVKLAVVGSPLPRPMKNVVGLGFCTDMPELYRAADFTIMASLYEPFGLVGVESVLCGT
                     RVVLSENMACTEVMNEEAGFFFSRQNPETLAQAVAQAVSLKKQGGHRLSDPMRALNYN
                     PSLSHHIDRLTDILASV"
     gene            complement(6552..8204)
                     /locus_tag="WJL87_001276"
     CDS             complement(6552..8204)
                     /locus_tag="WJL87_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ7799701.1"
                     /translation="MVAYVFLFLFVTAALVLIIRSHYRWTYFFASALFVFLAGGMLML
                     TAQWQRALNFASVWFVVLILFHRLKIHYYKQPLLISDFLLIADWRNWETLFHYKEAVI
                     GMAGLLALAAYAVFGWSGADALGLPWRWAGTVLFAAAFVSMRHFSKHPGAVKTWLDSL
                     PDDGRDVFLNLPMSCRAVFFQVPVFEGDGEAFAKQMPSETQPYGMSDEKPDIVVTLME
                     STLDPHCFDFAAAKIPDLKMFGRQEDTVFSSPLRVHTFGGATWKSEFAFLAGVPSTDF
                     GALASGVFYSVVPHLQTGFVRNLREHGYFCVALSPFTKGNYNAKAAYDHFGFNLMFQP
                     QDLGYPAPMGKNLWHISSEEMMQYARMILEKRHPDLENVRQPMFVYVLTMKEHGPYRT
                     DTDNVFDLDVPDLNAKTVSALNDYIGRIADLDKAVESFDRYLHERGKPFVFGYFGDHQ
                     VPFEGVSVRKKWDYAQPDYVTQFAVRSNIAGGFVQRQDFLDLAFAGGVLMEAAGLEAK
                     DGFMRANMAMRGLCGGGLEDCPNRKLVGDYRNYLYDVLKIAS"
     gene            8374..>8608
                     /locus_tag="WJL87_001277"
     CDS             8374..>8608
                     /locus_tag="WJL87_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ7799702.1"
                     /translation="MNYKIYDGAKIIKKENLSIGDYSQIDDFVFFNCGEKSILGSFVH
                     ISSFTSIIGGGEFYMDHFSGLSAGCRIITGSDDF"
BASE COUNT         2070 a         2621 c         2052 g         1865 t
ORIGIN      
        1 ttcaaataaa caggaatata acttcagaca aacaacttac cgcccgattt gtgctatcgt
       61 tttcgcacaa cttaaaaaac ctgacaattt tgtactttta ttacagagaa aggctttaca
      121 aatggacggc tggacacaga cgctgtccgc gcaaaccctg ttgggcattt cggcggcggc
      181 aatcatcctc attctgattt taatcgtcaa attccgcatc cacgcgctgc tgacactggt
      241 catcgtcagc ctgctgacgg ctttggcaac cggtttgccc acaggcagca ttgtcaacga
      301 cgtactggtc aaaaacttcg gcggcacgct cggcggcgtg gcgcttctgg tcggcctggg
      361 cgcaatgctc ggacgtttgg tcgaaacatc cggcggcgca cagtcgctgg cggacgcgct
      421 gatccggatg ttcggcgaaa aacgcgcacc gttcgcgctg ggcgttgcct cgctgatttt
      481 cggcttcccg attttcttcg atgccggact aatcgtcatg ctgcccatcg tgttcgccac
      541 cgcacggcgc atgaaacagg acgtactgcc cttcgcgctt gcctccatcg gcgcattttc
      601 cgtcatgcac gtcttcctgc cgccccatcc gggcccgatt gccgcttccg aattttacgg
      661 cgcgaacatc ggccaagttt tgattttggg tctgccgacc gccttcatca catggtattt
      721 cagcggctat atgctcggca aagtgttggg gcgcaccatc catgttcccg ttcccgaact
      781 gctcagcggc ggcacgcaag acaacgacct gccgaaagaa cctgccaaag caggaacggt
      841 cgtcgccatc atgctgattc ccatgctgct gattttcctg aataccggcg tatcggccct
      901 catcagcgaa aaactcgtaa gtgcggacga aacctgggtt cagacggcaa aaataatcgg
      961 ttcgacaccg atcgcccttc tgatttccgt attggtcgca ctgtttgtct tgggacgcaa
     1021 acgcggcgaa agcggcagcg cgttggaaaa aaccgtggac ggcgcactcg cccccgtctg
     1081 ttccgtgatt ctgattaccg gcgcgggcgg tatgttcggc ggcgttttgc gcgcttccgg
     1141 catcggcaag gcactcgccg acagcatggc ggatttgggc attcccgtcc ttttgggctg
     1201 tttccttgtc gccttggcac tgcgtatcgc gcaaggttcg gcaaccgtcg ccctgaccac
     1261 cgctgccgcg ctgatggctc ctgccgttgc cgccgccggc tttaccgact ggcagctcgc
     1321 ctgtatcgta ttggcaacgg cggcaggttc ggtcggttgc agccacttca acgactccgg
     1381 cttctggctg gtcggccgcc tcttggacat ggacgtaccg accacgctga aaacctggac
     1441 ggtcaaccaa accctcatcg cactcatcgg ctttgccttg tccgcactgc tgttcgccat
     1501 cgtctgacag acggaaagga tagtaaatga ctacgcattt tgtcgttatg ggcgtatgcg
     1561 gctgcggcaa gaccaccgcc gcgctgtccc tgcagaaaca cctcggtcaa tgtccctatg
     1621 ccgaaggcga cgagttccac acccaagcca accgcgacaa gatgggcgcg ggtattccgc
     1681 tgaccgatga agaccgctat ccgtggttgg gcaatctgcg cgactggatg acgcaacagg
     1741 cgcaaaacgg tgcgaaccac accatcgtaa cctgttccgc cctcaaacgc ggctaccgcg
     1801 acattctgcg cggagccgaa ggcaaagctg ccttcatcca cctcagtccg ccgcaagaca
     1861 tcaacctcga gcgcatgatg tcgcgcaaag gacattacat gaaagcaggg atgctcgatt
     1921 cgcaactgga aatcctcgag gaactgggcg aaggcgaata cggggtcaaa atcgccaacc
     1981 ccggcacgcc cgaagcggtc gaagccgata ttctgaactg ggttgcctcg gaaaacctgc
     2041 tttgaagcaa tatgccgtct gaagcccgac acaggatggg tttcagacgg cataaacatc
     2101 gggaacagaa tggattacat tgatttatag tggattaaca aaaaccagta cagcgttgcc
     2161 tcgccttgtc ctgatttttg ttaatccact ataaaatgga aaatacccgg ctatcgtctc
     2221 attttcgttt taatcagcca taaaaatgcc gtctgaaacc ctttcagacg gcatttctgt
     2281 caaacgccgg acgcactcaa tccaaactca acagcaggtt gcggaacgcg ttcgggtctt
     2341 tgataaacgt catctcgccc gcctgcggaa aatgaaaatc caacaggcgc gacacccaaa
     2401 aacggatgca gccggcgcgt tgggcggtcg gaaaatacgc cttttcttcg gcactcaagg
     2461 ggcgcacgcc ctcataaccg ccgataaacg cctttttcaa cgcctcatcc aacttattgt
     2521 ccgccgtcct tgcccaatcg ttgaccgcaa tcgccaagtc atacataaaa ttgccccggc
     2581 aggcataata aaaatcgata aagcccgaca cctgaccgcc gtcaagcaac acattgtctt
     2641 taaacaaatc ggcgtggatg atgcccgaag gcagatgatt gccgagattg tccttcagcg
     2701 catcgatttc ggaacacagc agcgcagcat cgtcttgcga caggacgggc agcagccggg
     2761 cgcacgcctc cgtccaccac gcatcgtaac gcgggttttc catttccaaa gggaaatcgg
     2821 cggcgacaag gtgcattttg gccaacatcg caccggtatg gaaacactgc tccgccgtcg
     2881 gcagcgcagt atccgaacct ttcaggcagg caaccaagca ggcgggcttg cccgccaaaa
     2941 cggaatcaag ccggccgtct ttgcgcgcaa ccggcgcggc aaccgccacg cccttcatac
     3001 tcaaatgccg gttaagctcc agaaaaaacg gcagctcttc ctgtttcaac acttcaaaca
     3061 cggtcagcac ataacgtccc gaagtcgtcg tcagaaaata attgctgttg gtaatcccct
     3121 gcgcgatgcc ctgcagggaa acaaattccc ccaaatcgta accgctcagg aagccgcgca
     3181 tttcatcatc ggaaacactg gtatagacag acatatcaac tcacttgctc aaaaacgggc
     3241 aaacccgccg tcagaacgcc ggacggcagg caaacatata attcacatcg gtcgaatcgc
     3301 acagggcata agtttgcgac aacacatggt aagtcatacc cttcgtatcc gccacatcca
     3361 agcctgcctg acggcacatt cgcgccagct cggcaggtgc gatgaatttt ttccagtcgt
     3421 gcgtgccttt ggggacaaac ttcaacagat attccgccgc cacaatcaga tgcaggtacg
     3481 atttcgggtt tttattgatg gtagaaaaaa acaccatgcc gtccggtttg accagattgg
     3541 cacaagcacg cacgatggcg gcgggatcgg ggacgtgttc catcatttcc atgcacgtta
     3601 ccacatcgaa cgagtgcggt tccgcctcgg caaggtcttc cacgcggata cattcgtatt
     3661 cgatatcggc gacattgttc aaagccgcgt gcaggcgggc ggtttccagc gactgctccg
     3721 ccatgtcgat accctttaca aacgccgcgc cgcgccgcgc catgctttcc gccaggatgc
     3781 cgccgccgca gcccacatcc aaaacccgtt tgccgcacaa atccgcgtgt ccgtcgatat
     3841 aatccagccg cagcggattg atgtcgtgca aggttttgaa ctcgcccgac ttgtcccacc
     3901 atttgtcggc aatccggctg aatttggcga tttccccctc atcgacatta tattttttgt
     3961 cggacatttt ccctcccatc tgacgaaccg cccactccaa aacccaagat tatactatgg
     4021 aacgcaatgc cgtctgaaag ccttttcggg cggcgcggcg caaatacctt acaaatcctt
     4081 acactttacg gcataatggc ggctcgcttt ttctggcaga aagacaaaat atgcccacca
     4141 aaaccccttc actgttcggc tgcgcgatga ttatcgccgg cacggtcatc ggcgcaggta
     4201 tgctcgccaa cccgaccgcc acatccggcg tatggtttac cggctcgctg gccgtgttgc
     4261 tgtacacctg gttttccatg ctctccagcg gcctgatgat tttggaagtc aacacccact
     4321 acccccacgg cgcgagcttc gacaccatgg ttaaagacct gctcggacgc ggctggaaca
     4381 tcatcaacgg tatcgccgtc gccttcgttt tatacctgct tacttacgct tatatcttcg
     4441 tcggcggcga cctgaccgcc aaaggcatcg gcaacgcctt gggcggcaat gtttcgctta
     4501 ccgtcggaca actcgtcttc ttcggcatcc tcgccttttg cgtatgggca tccgcacgct
     4561 tggtcgaccg attcaccagc gtcctcatcg gcggcatggt attaaccttt atttgggcaa
     4621 ccggcggcct gattgccgat gccaaaatgc ctgttttgtt tgacacccaa gcccctgccg
     4681 gcaccggcta ctggatttac gccgccaccg ccctgcccgt ctgcctcgct tccttcggct
     4741 tccacggcaa cgtttccagc ctgctcaaat actttaaagg cgacgcgccc aaagtggcta
     4801 aatccatctg gatgggcaca ctgattgcgc tggtaattta cgtcctctgg caaaccgcca
     4861 tccaaggcaa cctgccgcgc aacgagttcg cccccgtcat cgccgccgaa gggcaagtct
     4921 ccgtcctcat cgaaaccctg tccaaattcg cccaaaccgg caatatggac aaaatattgt
     4981 ccctgttttc ctatatggcg atcgccacct cgtttttagg cgtaacgctc ggactcttcg
     5041 actacatcgc agacatcttc aaatggaacg acagcgtgtc cggccgcacc aaaaccgccg
     5101 cgctgacctt cctgccgccc ctgatttcct gcctgctctt ccccaccggc ttcgttaccg
     5161 ccatcggcta cgtcggcctg gcggcaaccg tctggacagg catcatcccc gccatgctgc
     5221 tctaccgttc gcgcaaaaaa ttcggcgcag gcaaaaccta taaagtttac ggcggcttgt
     5281 ggctgatggt ttgggtcttc cttttcggca tcgtcaacat cgccgcacag gtattgagcc
     5341 aaatggaact cgtccccgta tttaaaggat aaaggcaaaa tgccgtctga agccctgttc
     5401 agacagcctt acgacattcg gaaaatcata tggaactcaa aatagacatt gcaaccaaca
     5461 acttcaaaca cggcggcggc acggaacgct acacattgga tttggtaaag ggtctgaaca
     5521 gacaaaacat cacaccggcc gtttatgcga cgaaatttga tcacggcatt cctgaatacg
     5581 ccatgatcga accccatctt gtcgatcaac accggacgct gaaaaaacta cgctcattcc
     5641 tcttttcaag tcggctcgct caaaccagaa aaaacagtgc cgccaaactg attgcctgcc
     5701 accacgccga ttacgccgac ctcctcatct gcggcggcac acacttgggc tacctgcacc
     5761 atatggcgca aaaaccgaac ctgctcgacc gtctcgccat acgccgcaac cgcagcaact
     5821 acgccaccgc caaactgatt atggcgcatt cccatatgat gcggcgcgaa ctggtcggac
     5881 tgtacggcgt tccccctgaa agaatccaag tcgccccccc ccccgcagat acggaacgct
     5941 tctttccaca acccggagaa actgccgacc tgcgcgccaa atacggcttt gccgaccatg
     6001 aaaccgtttt cctgttccca tcgaccggcc acacgcgcaa aggtctggaa ctgcttgccg
     6061 actttttcga acataccggc ctgcccgtca agcttgccgt tgtcggctcc ccgctccccc
     6121 gccctatgaa aaacgtcgtc ggactgggct tctgcaccga tatgcccgaa ctctaccgcg
     6181 ccgccgactt taccattatg gcctccctgt atgaaccctt cgggctggtc ggtgtcgaat
     6241 ccgtcctatg cggcacacgc gtcgtcctct ccgaaaacat ggcatgtaca gaggtcatga
     6301 acgaagaagc cggattcttt ttctcacgcc aaaacccgga aaccctggcg caagccgttg
     6361 cccaagccgt cagccttaaa aaacagggcg gacaccgcct gtccgacccg atgcgtgcac
     6421 tgaactacaa cccgtccctg tcgcaccaca tcgaccgact gaccgacata ctggcatccg
     6481 tataacaaaa tgccgtctga acgagttttc agacggcatt tttgcaaccg ttgcagccgt
     6541 gcggcaaaaa gctaactggc aattttcaaa acgtcgtaca gatagttccg atagtctccg
     6601 accagtttcc ggttcgggca gtcttccaac cctccgccgc acaaaccgcg catcgccata
     6661 ttcgcacgca tgaagccgtc tttggcttcc agaccagccg cctccatcag cacgccgcct
     6721 gcaaaggcaa ggtcgaggaa atcctgccgc tgtacgaatc cgccggcaat attgctcctg
     6781 acggcaaact gcgttacata atccggctgc gcgtaatccc atttcttcct gacggacacg
     6841 ccctcaaacg gtacctgatg atcgccgaaa taaccgaaaa caaagggttt gccgcgttcg
     6901 tgcagataac ggtcgaaact ttccaccgct ttgtccaaat cggcaatgcg cccgatgtag
     6961 tcgttaagcg cggatacggt tttcgcattc aaatcaggta catccaaatc aaacacattg
     7021 tcggtatccg tccgatacgg cccgtgctcc ttcatggtca gcacatatac gaacatcggc
     7081 tgccgcacgt tttccaaatc gggatggcgt ttttccaaaa tcatccgcgc atactgcatc
     7141 atttcctcac tggaaatatg ccacaggttt ttgcccatcg gcgcgggata gcccaaatct
     7201 tgcggctgga acatcagatt aaagccgaaa tggtcatatg ctgccttggc attgtagttg
     7261 cccttggtaa acggcgagag cgccacgcaa aaataaccgt gttcgcgcag gttgcggaca
     7321 aaaccggtct gcaaatgcgg cacgaccgag taaaacacgc cgcttgccaa cgcgccgaaa
     7381 tccgtggacg gaacacccgc caaaaacgca aattctgact tccacgttgc gccgccgaaa
     7441 gtatgcacgc gcaaaggtga ggaaaatacg gtatcttcct gccgtccgaa cattttcaaa
     7501 tcgggaatct tggcggcggc aaagtcgaaa cagtgcggat cgagcgtcga ttccatcagg
     7561 gtaacgacaa tatcgggttt ctcatccgac atgccgtacg gctgggtttc agacggcatc
     7621 tgtttggcaa acgcttcccc gtcgccctcg aacacgggta cttggaaaaa caccgcccga
     7681 caggacatcg gcaggttcaa aaatacgtcg cgcccgtcgt ccggcagcga gtccagccac
     7741 gtctttaccg cgcccggatg tttggaaaaa tgccgcatcg acacgaacgc cgccgcaaac
     7801 aaaaccgtgc ctgcccaacg ccatggcaaa cccaaagcat ccgcaccgct ccagccgaaa
     7861 accgcatatg ccgccaaagc cagcagcccc gccataccga taaccgcttc cttataatgg
     7921 aacagcgttt cccaattccg ccagtcggca atcagcaaaa agtcggaaat caacaacggc
     7981 tgtttgtaat aatggatttt cagcctgtgg aacagtatca gcaccacaaa ccagaccgaa
     8041 gcgaaattca aggcgcgctg ccactgagcc gtcaacatca gcataccgcc cgccaaaaag
     8101 acaaacagcg cggatgcgaa aaaatacgtc caacggtagt gcgacctgat aatcagcacc
     8161 aacgccgccg ttacaaacaa aaataggaaa acataggcaa ccatcccgtc ccactcctca
     8221 aaacggcata cctgagccgt tgcgaattat tatcgaaagt gtgcattata atgcagcaca
     8281 ggcgcatcac tgccgatatt tcagtataat tattcgagat ggaatcactg aaaatggtga
     8341 agttacaatg agccctttta gaggaactaa aaagtgaatt ataaaatata cgatggtgca
     8401 aagattatta aaaaagaaaa tttatcaatt ggggattatt ctcaaattga tgattttgta
     8461 ttttttaatt gtggagagaa aagtatttta ggaagttttg ttcatatatc atcatttacc
     8521 agtattattg gtggtggaga attttatatg gatcattttt caggattaag cgcagggtgt
     8581 agaattatta ccggtagcga tgatttta
//
LOCUS       DAWKST010000081         8603 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.081, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000081 DAWKST010000000
VERSION     DAWKST010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.081"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(280..3132)
                     /gene="gcvP"
                     /locus_tag="WJL87_001278"
     CDS             complement(280..3132)
                     /gene="gcvP"
                     /locus_tag="WJL87_001278"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEZ7799703.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEKSMDEFVGNTVPQ
                     SIRMPSELDLPDALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFGFELVVSDFAKADDGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDAFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGINVVHKVFFDTVTVDFGNK
                     EKADQVFAAALESGYNLRRVNDTQVAAAFHETSACEDLVDLYRAFTGKDTFAFADDVK
                     GRLNAELLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTAGYRELLADMENSLKSITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAQRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIVHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHALTDTHSASADQT
                     AVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVGRGEWPKEDNPLVNAPHTASDVTGEWAHPYSREEAVFPL
                     PFVREHKFWPSVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3163..3522)
                     /locus_tag="WJL87_001279"
     CDS             complement(3163..3522)
                     /locus_tag="WJL87_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799704.1"
                     /translation="MTDRPSLLWIKPGQAEIMEYCEPEEASDPYATYRRASLMAMLPL
                     FVVILVLLNIVFPLLAHPLAWLVPAGFMVLVGGFPLSLPLVALLVLTCCILAHCPPLS
                     RLLCCPCPNHPMSKNSA"
     gene            3470..3700
                     /locus_tag="WJL87_001280"
     CDS             3470..3700
                     /locus_tag="WJL87_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ7799705.1"
                     /translation="MISACPGFIHSRLGRSVMPSEPAGRLFQKTPYRRNFMRLYGVGF
                     ALQTAYASRSSLVFQSVHDYINNLGADKPLFI"
     gene            complement(3693..4334)
                     /locus_tag="WJL87_001281"
     CDS             complement(3693..4334)
                     /locus_tag="WJL87_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ7799706.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(4324..4875)
                     /locus_tag="WJL87_001282"
     CDS             complement(4324..4875)
                     /locus_tag="WJL87_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ7799707.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLNWTLMLKKRQAFQTAFEGFDIDTVAAFDDTDRERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDTHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAI"
     gene            complement(4879..5397)
                     /locus_tag="WJL87_001283"
     CDS             complement(4879..5397)
                     /locus_tag="WJL87_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ7799708.1"
                     /translation="MRLFPIAAALSLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFIPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5397..7037)
                     /gene="pqiB"
                     /locus_tag="WJL87_001284"
     CDS             complement(5397..7037)
                     /gene="pqiB"
                     /locus_tag="WJL87_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEZ7799709.1"
                     /translation="MTDNSPPPNGHAQAHVRKNNTFLSAVWLVPLIALIAGGWLWIKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGITLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSSSPKLRPHTVYAGDTVIATQGGGLDDLQVKLADLLDKFGKLPLDKTVSGLN
                     ASLAELKSTLKSINTLSNNPQTQNIPNELNQTLKELRTTLQGVSPQSPIYGDVQNTLQ
                     SLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(7030..8490)
                     /locus_tag="WJL87_001285"
     CDS             complement(7030..8490)
                     /locus_tag="WJL87_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ7799710.1"
                     /translation="MGSAVGGQPLEKIFLPDDKGLFILPNLPVLHPDWVYRLFSFIMP
                     PLIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCRADVPRLDRGEAAFCPR
                     CGYKLFRVGRHPFSAPPAYAAASLILMAFAYGMTYIEVGIPGAASVLSLPEMMRLMVF
                     QDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKRVYPALRLATRVMVRLRQAMMVD
                     VFFVSTLVAYIKLSSVAEVRFGSAFYLMFALSVMLIRTSVSVPQHWVYFQIGRLTGDN
                     AVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLSISSAFLTAAVILYFPA
                     NILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSASILVPVLKIAAMSVLIA
                     SARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFHTYAARVIPGSAAVYFC
                     LVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKHD"
BASE COUNT         1926 a         2200 c         2433 g         2044 t
ORIGIN      
        1 gaggcaacgc tgtactggtt tttgttaatc cactatattt gtttgtccgt gtggaaatga
       61 catcaaaggg tgtgaatagg cttttcatct ttgaaatctg tttgcacgat cggatgaata
      121 aaaagtagga tgataaaaaa gctgatgtat ggatactggt ttaactcatt tgttttaaag
      181 ttatagtcga gatacgtgta ttggtgcaac cagtctgccg ttgacgaaaa tgccgtctga
      241 aaatgtttca gacggcattt gtcttcgggg tttcaacggt cagtcttcat aattttccat
      301 cggtgggcag ctgcacacga gattgcggtc gccgtacacg tcgtccacac ggttgacgct
      361 cggccagaat ttgtgttcgc ggacgaatgg caacggaaag acggcttctt cacgggagta
      421 cggatgcgcc cattcgccgg ttacatcgga tgcggtatgc ggagcgttga ccagcggatt
      481 gtcctctttc ggccattcgc cgcgcccgac tttcagcact tcctgtttga tttgtttcag
      541 ggcggcgatg aagcggtcga gttcggcttt gctttcgctc tcggtcggct cgatcatcag
      601 cgtaccggca acggggaagg aaaccgtcgg ggcgtggaag ccgtagtcca tcaggcgttt
      661 ggcgatgtcg gtttcagtga tgccgctttc ggctttgagc ggacgcaaat cgacgataca
      721 ttcatgcgcg acgcggccgt ttttgcctgt atacagaatc ggatagtctt cgctcaggcg
      781 tttggcaacg tagttggcgt tgagcaatgc ccagcgtgtc gcctgttcca tgccttgttt
      841 gcccatcatg gtcaggtaca tccaagtaat cggcagtatg gacgcagaac cgaaggccgc
      901 agcagcaacg gcggtttggt cggcactggc gctgtgggtg tcggtcaaag cgtgtcccgg
      961 tgcgaacgga gccagatggg ctttcaagcc aatcggaccc atgcccggac cgccgccgcc
     1021 gtgagggata cagaaggttt tgtgcaggtt catgtgcaac acgtccgcgc cgacttcggc
     1081 aggctgcatg atgccgattt gggcgttgag gttggcgccg tccatgtaaa cctgtccgcc
     1141 gttttcatgg acgatgcggc agatgtcgcg gatgccttct tcgtacacgc cgtgggtgga
     1201 cggataggta atcatgatgg cggacaaagc gtcgcggtgt tgctcggctt tggctttcaa
     1261 atcgtcgatg ttgacgttgc cgtgttcgtc ggtatcgacg acgacgactt tcaaaccgag
     1321 catggcggcg gtggcggggt tggtgccgtg ggcggatttg ggaatcagac agatgttgcg
     1381 ctgcgcttcg ccttgcgctt cctgatagcg gcggatggca agcataccgc tgtattcgcc
     1441 ctgcgcgccg gagttgggct ggaaggaaat tgcatcaaaa ccggtgatgg atttcaggct
     1501 gttttccata tcggcgagca attcgcggta gccggcggtt tgcgcttcag gagcgtaagg
     1561 atggatgtcg gagaactccg cccaagtaat cggcaacatt tccgcagtcg cgttgagttt
     1621 catggtgcag ctgcccaatg aaatcatgct gcggttcatc gccaagtcgc ggtcttcgag
     1681 ttttttcagg tagcgcagca tttcgtgttc ggtgtggtaa cggttgaaca cgggatgttg
     1741 cagaatgtcg tcttgacgca gcagttcggc gttcagacgg cctttgacat catcggcaaa
     1801 tgcgaacgta tccttgccgg taaacgcgcg gtacaaatca accaaatctt cgcatgccga
     1861 tgtttcgtgg aatgcagccg caacttgagt atcgttaacg cggcgcaggt tatagcccga
     1921 ttccaaagca gcggcaaaca cttggtctgc tttctctttg ttgccgaaat cgacggtaac
     1981 ggtatcgaag aagactttgt gaaccacatt aatgccgtct gaaaccagcg catcggcaaa
     2041 agcagaagcc agcgcgtgaa tgcggttggc gatgcgtttc acgccttcag ggccgtggta
     2101 aacggcatac atacccgcca aattcgccag caatgcttgt gcggtacaaa tattggacgt
     2161 ggctttttcg cggcggatgt gttgctcgcg ggtggacaac gccatgcgca aggcaggttt
     2221 gcctgatgcg tctttggata cgccgatgat gcggccaggg gcggaacgtt tgaacgcgtc
     2281 tttaaacgcg aaataagcgg cgtgcggccc gccgaagccc ataggcacgc cgaaacgttg
     2341 ggtgttgccc aaagcaatat ccgctcccaa ttcggcaggt gatttcagta aaaccaagct
     2401 catgatgtcg gcggcaacag caacgatcgt gccttttgct ttcagacgac cgataacatc
     2461 ctgcaagtct tgcacatcgc cgtctttgcc gacgtattgg aacagcgcgc cgaagtattc
     2521 gccgtcatcc gctttggcaa aatcgctgac caccagctcg aagccgaaat atttggcgcg
     2581 ggttttcatc acgtccaaag tttgcggata cacgcgcgca tccacaaaga aacgctcgga
     2641 tttcaccttg cccacgcggt gcgccatcgc catcgcttca gccgcagcag ttgcctcgtc
     2701 cagcaaagac gcgcccgcca cagggaaacc ggtcaaatcg atacacactt gttggaagtt
     2761 taacaacgct tccaaacgac cctgggcaat ttccgcctga tacggcgtgt aggcggtgta
     2821 ccaacccgga ttttccaaca cgttgcgcaa aatcacgttc ggcacgcggg tcgggtaata
     2881 gcccaaaccg atataggact tgttgatcac gtttttcgac gcaatgcctt tcaatttcgc
     2941 caacgcgtcc gcttcggtca gggcatcggg caaatcgagt tcggacggca tacggatgct
     3001 ttgcggcacg gtattgccga caaattcgtc catgcttttc tcgccgacag cggcaagcaa
     3061 cgccgcttcg tcgccgaaac tcaaatggcg cgcggcaaat tcgttcgggt tgaacaattc
     3121 agatagtttc atttctactc cttcgggttt gtagggctgc catcaggcag aatttttaga
     3181 catcggatga ttcgggcaag ggcagcacaa aagacgggat aatggcggac aatgcgccag
     3241 aatgcagcag gtcaggacaa gcagcgccac aagcggcagc gataagggaa agccgccgac
     3301 caaaaccatg aaacctgcag gcaccagcca agccaaggga tgcgccagaa gcggaaaaac
     3361 aatattgagc agaaccaaaa tcacaacaaa cagcggcagc atcgccatca ggctggcacg
     3421 cctgtatgtt gcgtacgggt cggatgcttc ttcaggttcg caatattcca tgatttcagc
     3481 ctgtcctggc tttatccaca gcaggctcgg acggtcggtc atgccgtccg aaccggcagg
     3541 ccgtcttttt caaaaaacgc cataccgccg caacttcatg cggctgtatg gcgtaggttt
     3601 tgcacttcag acggcatatg cgtcccgaag cagtttggtg ttccagtcgg ttcatgatta
     3661 tataaacaac ttgggtgcgg ataaaccttt gtttatctaa acccctgttt tctcaaaaat
     3721 tgattcaaat cttcggcaaa tttccgatag cctttgccgt tggcgtggat ttggtcggat
     3781 ttcagattat tatcgcccaa aatttccgcc cacgcgccgc cgaacagcgg aatgccgtat
     3841 tcctcggaca aatcctcata cagcggatga tcgctcaaat gcccgaacaa cgcgcccagt
     3901 gtgatgtgcg gcacgccgac gaggacggcg gggatgtttt ccttctgcac ggtttcgatg
     3961 attttcgcga tattggcgcg ggtctgctcc ttgggaactt tgcgcagaaa gtcgttgccg
     4021 cctatgccga caatcacaag cttgggtttg cgtgccaaca gcgcgggcag gcgcgacagg
     4081 gcttgggcgg atgtatcgcc cgatacgccg ccgttgacaa tattccaacc cgtcagtttt
     4141 tgcagttgcg cggggtagga ttcgccaggg tttgcgccgt agccgaaggt aagcgaatcg
     4201 cccaaggcaa gtacggtgct tccttcggga attttggcgt gggttcgggc ggattttctg
     4261 ccgcaggcgg taaggagcaa cgcgcctgcg ccgaggagga aggttcttct gttcattggt
     4321 ttttcagatg gcatcgagcc atttcgggtg gtatttcagg gtttcacggt aaaccggaca
     4381 gctttcggcg tgcgcgcctt tgaggtagcc ggtactcatc agaaattcgc cgacgatttc
     4441 gccgccgacg aatttgaaat gttttttaaa gagtttaacc cattcgtctt tgcttcgcgg
     4501 atggtgcgtg tcgagccagt tcttgaacga gccgtattct tgttgcaacg cttggatttg
     4561 ccgtgcattg aaaatggcgg catcgatttt caggcggttg cggacaatgc ccgcgtcggc
     4621 aagcaggcgt tcgcggtcgg tgtcgtcgaa ggcggcaacc gtatcgatgt cgaaaccttc
     4681 aaatgccgtc tgaaacgcct gccgcttctt cagcatcagc gtccagttta atcctgcctg
     4741 attgatttcc aacaccagcc gctcaaacaa ttcattgtcg tcctcaatcg gaaaaccgta
     4801 ttgcgtgtcg tggtaatgtt tgttcgggtt atcggtgttt tcgggaagtg aggcggcaaa
     4861 ttcgcaatag ttcacgtttt actcgaccat ctgttgcgcc gcctgtttca gtccctgttc
     4921 gagtgcggcg gtcatcgcgg cgtagccgtc accctgctgt tcggtttcga tatggaaggg
     4981 tctgttcgta ccgtcgggta ggacggcgta gccgctgatg agggttttgc ccgtgtagct
     5041 gccttggaat gcgtcaatat agaccgtcca tttttcggta ctgccgctgc gtgaggcagg
     5101 aataaagatg cgtgtgctgt ccaaacggtt gaatgcattg ctcaacgccg cttcgagcat
     5161 atcgtccaag gtgtccgccc aaacgtggtt ttgtgcggtg ttgaggcggt aggggtcggt
     5221 ttgatagacc agtccgccgc gtttgagcgg ttcggcaaga cggacttcga cggcagtttc
     5281 gccgccttgc gttgcaggac ggatgtagcg gctgtcgggc aacacgaaat attgtgtgct
     5341 ttgcacagta ccgcaggcgg caagcgacag ggcggcggca atcgggaaaa ggcgcattat
     5401 cggcttcctt tcgggatagg gtctttgctg ctgctgttga aaatcagcgc gttgggtttt
     5461 tctttcaaag tgttaatgac gggttgaacg tcttttaagg ttttgtccaa actttgcagc
     5521 gtattttgta cgtcgccgta gataggcgat tgcggcgata cgccttgcag ggttgtgcgc
     5581 aactctttca gggtttggtt caactcgttc ggaatgtttt gcgtttgcgg attattgctt
     5641 agcgtgttaa tggatttcag cgtggatttg agttcggcga gcgaagcgtt taaacctgaa
     5701 acggttttgt ctaaaggcag tttgccgaat ttgtccagca aatccgccaa tttgacctgt
     5761 aagtcgtcca aaccgccgcc ttgtgtagcg atgacggtat cgcctgcata aacggtatgc
     5821 ggtcgcagct tgggcgaaga cgaaggctga tcgttcaact caatcatttt gctgccggtc
     5881 agcaggttgt tgctggagat ggtggcggtc aggcctttgt ttaaagccgt ctgaaattgt
     5941 tgtttccaat gctctttgct ttgttcgtcg gcattgattt ccaaacggga aggctcgatg
     6001 cggatgcgta cgggaatcca gccgttttca aataaatgca ggctgtcgtt gcggtcaaaa
     6061 taagggacat cggaaaccac gccgacattc agccctttgt actcgacggg cgaaccgacg
     6121 gtcaggccgc gcacggattg tttgaaaaac gcggtgtagt acagcgagcg gtcgtcaggc
     6181 aggttggcga cttcgctgcg gctgtcgtaa agcgtgaagc tgtcttcgct tttgacgttt
     6241 ttactgtttt tggttttcgg cgaatcaaat gaaatcgcgc ccgacagcag ggcaggcaga
     6301 ggggcggaat tgagtgtgat gccgctgcct gtggtttcga tattgatgcc gctttccagc
     6361 caaaaacggc tggcggaatg aatcagtttg tcgttggggc tttggatgaa gatggtgtaa
     6421 tgcacgcttt ggtcggacgg gtcgaaatgc gcgctttcga cttgcccgac cataaagttt
     6481 tcatacaaaa cagggctgtt gacgttgagg atgcggtcgt ttttaccaat caaattcaag
     6541 cgcagcccgc tttgcccgat ggcggtaacg ggcggaatgt cctgcacttg gaacacgtct
     6601 tttgcctcgt cgcttttgcc gggtgtaaaa gcgatgtacg aacccgaaag cagcgtaccc
     6661 aaaccggtta cgccgctttg gtcgatacgc ggcttgacca cccaaaactg ggtatcgctg
     6721 cggatgaggc cggatacgtc cgcattgagt tgggcggtta cttccacgcc tttttggtcg
     6781 tcgcgcagtt tgattcgggt aacgcgtccg acatcgatgc tcaatacttt gatgaccgta
     6841 ttgttgacct caatgccttc cgcgctgtcc atcaagagcg taaccacagg ccccctgttg
     6901 cggatttcct taatccacag ccagccgccg gcaatcagcg cgatcagcgg aaccagccag
     6961 acggcagaga ggaaggtgtt gtttttgcgg acgtgtgctt gggcgtgtcc gtttggagga
     7021 gggctgttgt cagtcatgtt tttccgtttc gttgaaagca atgccgtctg aagcgcgttt
     7081 gtcccaaagc aggcgcgggt cgaaataata ggcggacagc atcgtcagaa tcacgaccag
     7141 gcagaaatag actgccgcac tgcccggaat gacgcgcgcg gcataagtgt ggaacgaaca
     7201 cattaaaata ataatcacaa aaatatcaat catcgaccag cggccgaccg cttcggtgat
     7261 gcggtagagg tgtgacaatt tctttgcgcc tgtcggcaaa gcgaagcggg cggacgcaat
     7321 taaaaccgac attgccgcaa tcttcagcac cggcaccaaa atactcgcgc tgaaaataac
     7381 cgccgcaatc agcctgtcgc cctcgtccca catataagcg atgccgttaa ggatggtatt
     7441 gacctccgtg gcggcaggat tggacgaaat cataatcggc aggatattgg cagggaaata
     7501 caaaataacc gccgccgtca gaaacgccga cgaaatactc agacttttcg gccgtcggcg
     7561 gtacagttcc gcgccgcaca caccgcaggg ggattcggca ctgtcgcgga aatacaggca
     7621 gcggctgcaa caggttttgc cttccgatgc cgtctgaacc gcattatccc ccgtcagccg
     7681 cccgatttga aaatacaccc aatgctgggg aacggatacc gaagtccgaa tcagcataac
     7741 cgacagcgcg aacatcagat aaaacgccga cccgaagcga acctctgcca cagacgagag
     7801 cttgatatac gccaccagcg tggaaacaaa aaacacatcc accatcatcg cctgcctcaa
     7861 gcgcaccatc acacgcgttg ccaaacgcag cgcaggatac acccgtttcc gtatcagcgc
     7921 ggcatagaca tacaggcaca gcagcagaaa cagaaccggc gcgccgaagg tcagcacaaa
     7981 catcacttcg gccaaaaaac cataatcctg aaacaccatc aggcgcatca tctcgggcag
     8041 cgaaaggacg gatgccgcac ccggtatccc gacctcgata tacgtcatac cgtaagcaaa
     8101 cgccatcaaa atcagcgaag ccgccgcata agcgggcggg gcggaaaaag gatgcctgcc
     8161 caccctgaag agcttatagc cgcagcgcgg gcagaacgcc gcctcccccc tgtccaaccg
     8221 aggcacatcc gcgcggcagc cgcattccgg gcaatctacc gtatgcggcg gtagggcttc
     8281 gtcgcggcag ttgccgcgaa ggctgtgggg gatattttcg gcaaacggct tcatcgggaa
     8341 agtggcaaat ataagtggcg gcattataaa ggaaaaaagg cgatacaccc aatcaggatg
     8401 caaaaccggc aggttcggca gtatgaacaa acccttgtcg tccggcaaaa atattttttc
     8461 gagcggctgt ccgccgactg ccgaacccat ttgataaaat cccgattctg taaagaatag
     8521 taaaatacct gcttaaaaac atcacgcaaa tgatattttt tccaaaaata tgtgataata
     8581 aatttcgttt atgattcttt att
//
LOCUS       DAWKST010000082         8514 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.082, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000082 DAWKST010000000
VERSION     DAWKST010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.082"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            83..1267
                     /gene="coaBC"
                     /locus_tag="WJL87_001286"
     CDS             83..1267
                     /gene="coaBC"
                     /locus_tag="WJL87_001286"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ7799711.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMQIVERLAVYL
                     NK"
     gene            complement(1334..3490)
                     /locus_tag="WJL87_001287"
     CDS             complement(1334..3490)
                     /locus_tag="WJL87_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ7799712.1"
                     /translation="MPAPQLSAPYDPLTAEARALLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTVAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDSCPTLLKSDPE
                     QQLDADWENMNGQNYRVVLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLDQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3583..3789)
                     /locus_tag="WJL87_001288"
     CDS             complement(3583..3789)
                     /locus_tag="WJL87_001288"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ7799713.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(3848..4465)
                     /gene="gmk"
                     /locus_tag="WJL87_001289"
     CDS             complement(3848..4465)
                     /gene="gmk"
                     /locus_tag="WJL87_001289"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ7799714.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEVNGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRNALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            4593..5192
                     /locus_tag="WJL87_001290"
     CDS             4593..5192
                     /locus_tag="WJL87_001290"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ7799715.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYHLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(5238..6026)
                     /locus_tag="WJL87_001291"
     CDS             complement(5238..6026)
                     /locus_tag="WJL87_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ7799716.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALCGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVHEVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEQEGLKTVRWHEEAVDLLPAGASKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPGVDEDGVLRGLQDLGVI"
     gene            6351..7706
                     /gene="yegQ"
                     /locus_tag="WJL87_001292"
     CDS             6351..7706
                     /gene="yegQ"
                     /locus_tag="WJL87_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ7799717.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHDRNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQQCPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHHTQDYQNYLTGHSTAKQSQYVGHVTEIDKNGWATVEVKNR
                     FAVGDSLEIIHPGGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPNMQGKEKALIARVLN
                     P"
     gene            7840..8316
                     /locus_tag="WJL87_001293"
     CDS             7840..8316
                     /locus_tag="WJL87_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HEZ7799718.1"
                     /translation="MPAVCQSGSAPKNAHTASAGTPAGESCKVIRDDTAGSGKNIIYR
                     CSNGQAAINAAIADGVLESGIPVSFGGGGVPVANGRNLVTRQAANGVGKSDSEACEHA
                     LINAARKFQQTAGKLGGRSVTGFHSYFDKQPLQGGQYDCQTGSFHVRVVMRANVVR"
BASE COUNT         2161 a         2329 c         2018 g         2006 t
ORIGIN      
        1 ttgtatccaa acggtatcta atgtatccac actttgttat agaatggctg ctgttttttc
       61 ttcgtaatta gaaattgtca aaatgggcaa acatattctt ttaggtgtaa cgggcagtat
      121 tgcggcgtat aagtcttgcg agttggttcg tctgctgaaa aaacaggggc attcggttac
      181 ggtggttatg agccgctcgg caactgaatt tgtttctccg ctgacttttc aggctttaag
      241 cggcaatcct gtcctgaccg acacgcacgg cggcaacggt tcaaacggta tggaacatat
      301 caacctgacc cggaatgcgg atgtttttct gattgcgccg gcaagtatga ataccgtggc
      361 aaaaatctgt aacggcgtgg cagataacct actgaccagt ctggcagccg cacggaaatg
      421 tccgcttgcc atcgctcccg cgatgaatgt ggaaatgtgg ctcaaccctg ccaaccaacg
      481 gaatatcgca caactggttt cagacggcat tactgtctat atgccgggct tgggcgaaca
      541 ggcttgcgga gaaaatggca tgggaaggat gccggaacct gccgaattgc tggatttgct
      601 tccggacttg tggacaccga aaattttaag gggcaagaaa atcttgatta ccgcaggtgc
      661 gacatttgaa gccattgacc ctgtcagggg catcacaaat acctccagcg gaaaaatggg
      721 ggtagctttg gcgcgggcgt gccgtgccgc cggtgcggaa gtcagcctga ttcacggaca
      781 gcttcaaacc gcgctgcctt tcggcatatc cgatacggtt caagccgtca gcgctgaaga
      841 tatgcatcgc gcagtgcatc gtttgattga aaaacaagat gcttttattt ctgttgccgc
      901 cgtctcagac tataaggtta aaaacaggag tacacaaaaa ttcaaaaaag ataaaaatgc
      961 caaaccgtta tccatcgaat tggatgagaa ccccgatatt ttggcttcta ttgcctcatt
     1021 gccgaacccg ccgttctgca tcggttttgc cgctgaaacg gagaatgtaa tggcatatgc
     1081 acgagaaaaa cgtattaaga aaaaaatacc ggtgatagtt gccaatgatg tttcaatcgc
     1141 gatgggcaaa ccgaccaacc ggattaccat tatcggggac gacggggaac tatcttttcc
     1201 cgaaacaagt aaagatgaag cggcgatgca gattgttgaa aggcttgccg tatatttgaa
     1261 caaataagca attgaacgga taaaccataa aacgggttgc ctgttaatca aaaggcaacc
     1321 cgttttacct gcttcaactt ctgatgactt tgcggatata tggaatacta tgcagatttt
     1381 gaataatctg attcaactga tccaaattct tgactttcaa taagaatttg aattcgacaa
     1441 aaccttccgt tcccgactgg gatttagacg gtgtttcgac cgactcgatg tccgcaccgg
     1501 aatcggaaat cgcttgcgcc attaatgcca acaggccgtg gctgtcttcc gattggactt
     1561 gaagcacgac acggtagttc tgcccgttca tattttccca gtctgcatcc agctgctgtt
     1621 cgggatcgga cttcaacaac gtcgggcagc tatccctatg gataatcatg ccttttccct
     1681 taaccaacag caaacggatg gaatcgccgg gaacagggtg gcagcactct gcaaaatgaa
     1741 tatgcccgct ttcctgacca tcgactttaa tggaactgag cctgacctcg ctgccgaaat
     1801 gctcccctgc caactcagca atgtgcatgg cgacataaac aggcagggta tgccccatcc
     1861 ctacgttgta cagcacttct tcaaacgatg tctgcttgtc gttgagatcg gcaagatatt
     1921 tttccttgat gccgtctgaa agcaggacat ctttgggcag caaactggac agggcttttt
     1981 gtaagaggct ctctcccaaa acgaccgcat cgtgccggtt aaggttttta atatattggc
     2041 gtatggcact gcgcgccctg ccggacacgg cgaaattcaa ccacgcggga ttgggtttgg
     2101 cgtgttcgga tgtgataatt tcaacagaat caccggtttt gagcttcgta cgcaacggca
     2161 tcatgatatt gttgatacgt gcggcaacgg ttttgtgccc gatatcggta tgcaccgcat
     2221 aagcaaaatc gacaggcgtt gcccctttgg gcaaagttag gatttttcct tttggcgtaa
     2281 ggatgtagat ttcgttcgga aacaaatcga ctttgacgtg ttcgagaaac tcaatggcat
     2341 tggcactgct tgcctgcaaa tctaagatat ttttcagcca ccggtttgtg tgaagcaccg
     2401 cctgatcgac cgtcttagaa tatgatttat agctccaatg tccggcgatt ccaccttcgg
     2461 caacagcatc catttccttg gtacgtatct gaacttcaat cggcaagccg taagggccga
     2521 ccaaagtcgt atgcagactt tgatacccgt tgcttttcgg aatggcgata tagtctttga
     2581 accgcccggg cttgggctga tagagggtgt gcaatgcgcc gagtgcggca taacaggctg
     2641 gaatgctgtt gacaatgacg cggaaaccgt aaatatccat aacctcggca aagcgcagct
     2701 ttttcgccat cattttctga tggatgccgt acaggttttt ttccctgcct ttgattttgg
     2761 cctctatatt cgcgcctacc agccgctggc cgaatgcgcg caagactttg ccgacaacgt
     2821 cctgccggtt cttccggctc ttgtccatcg ctttttttaa agtctcgtag cggttgggat
     2881 gcaggttttg gaacgataaa tcctgaagct cttgatatgc gttattcaaa cctatacggt
     2941 tggcaatctg tgcatagatt tcaagggttt cccttgcaat ccggcggcgt ttgtccgggc
     3001 gcatcgaacc gagcgtccgc atattatgca gacggtcggc aagtttaacg acaatcacgc
     3061 gcacatcttt ggtcatcgcc aaaatcagtt tgcggaaact ctccgcctga tgttccgcat
     3121 gatcttcaaa tttgagtttt tcgagtttgg acagcccgtc caccatctcg gcaaccgtat
     3181 tgccgaacac cgccgccatt tccccttttg tcacgcccgt atcttccaat acgtcgtgca
     3241 tcacgcctgc acaaagaccc tgtatgtcca tatgccaaag ggcgagctgc gtcgcaacgg
     3301 caatcggatg cgtgatgtag ggctccccgc ttttgcgggt ttgcccgtcg tgggcgcgaa
     3361 acgcataggc gacagctttt tcaagctccg cctgttcctc gggcttgagg taggaggcgg
     3421 tatggaaaag cagggcacgc gcttcggcgg tcaggggatc ataaggggcg gaaagttggg
     3481 gggcgggcat ttcagacggc tttcggaaca tattttattt ttcgtttcaa accgtcggac
     3541 tgcacggcgg cagaatgtgc cgacgtgcgt ttccggcaga atttatttat tgcgcgtcaa
     3601 cagttccgta ccgatatgcc cggcggcgat ttcccttaag gcggtaacgg tcggtttgtt
     3661 attgcggaca tcgtccacaa gcggcgtatt gccattttca agctggcggg cccgacgggc
     3721 cgctaccaat gtcaggtcaa aatggttgga aatttttccg gtacagtctt cggtggtaat
     3781 acgtgccata ttatttgctt tctttcaaaa atatttaaaa taggaaactg ggtattttcg
     3841 cggtttttta ggaattttcc aacaaatctg caataaatcc cagttgccgc gaccttttca
     3901 gacggcaggc attcacaata tggcgcaaat cctcctccgc ccgcgccaag tcatcattga
     3961 ccacgacaaa atcgaacaat acggactgtt cgatttcatg ccttgccttc gacagcctcc
     4021 tttggataac ttcccgactg tccgtcccgc gtccgttcag acgtgcagca agcacatcga
     4081 aagacggcgg caggataaag atgccgacgg cttccggcag cgcgttgcga acctgcgccg
     4141 cgccctgaac gtcgatttcc aaaattacgt catagcctgc cgccgccaaa gcattcacgc
     4201 cctccgcgct tgtgccgtaa tagttgccga acacgtctgc gtattccaaa aaagcttcct
     4261 gcgcgataag cgactcaaac tcttctttag aaacaaaatg ataatgtacg ccgtttactt
     4321 cgccttcacg cggcggacgc gtcgtgtgcg atacggaaac gcgcaaaccg ttatggtttg
     4381 ccaacagccg cgacaccagc gtggttttgc ccgtgccgga agcggccgaa atgataaaga
     4441 tgttgccttt tcgataagcg gacatatttt ttacctgtat attttccagc cgattgtatc
     4501 acaacggaca ctcggtttcc tatttgccga tgcccatatt ttgccgctat tgttttgatt
     4561 gatttggcaa gcggcaggct gacggctaca atatggcgtt aaaaacatca aacttggaac
     4621 acgcaatgct ggttcatccc gaagctatga gtgtcggcgc gcttgccgac aaaatccgca
     4681 aaatcgaaaa ctggccgcaa aaaggcatct tattccacga catcacgccc gtcctgcaaa
     4741 gcgcggaata cttccgactt ttggttgatt tattggttta ccgctatatg gatcagaaaa
     4801 tcgacatcgt tgccggtttg gacgcgcgcg gcttcattat cggcgcggca ctcgcctacc
     4861 atctcaacgt cggttttgtc cccatccgca aaaaaggcaa gctgcctttt gaaaccgtat
     4921 cgcaaagcta cgcgctcgaa tacggggaag ctgcggtgga aatccacacc gatgccgtca
     4981 aacccggttc gcgcgtgctg ctggtcgatg atttggttgc cacgggcggc acaatgcttg
     5041 ccgggctgga actgatccgc aaactcggcg gagaaattgt cgaagctgcc gctattttgg
     5101 aatttaccga ccttcaaggc ggcaagaata tccgtgcaag cggcgcgccc ttatttaccc
     5161 tgcttcaaaa cgaaggctgt atgaagggct gaaaaccgac cctgccgtct gaaaccggca
     5221 gggttgttat tatgcgttca aatcacgccc aaatcttgca agcccctcaa cacgccgtct
     5281 tcatcaacgc cggggcaaac atatttcgcc gcttctttcg ccgcctgttc cccgttgccc
     5341 attgccacgc cgaacccgac ttctgacagc atttccacat cgttcaaacc gtctccgaac
     5401 gccatcacgt ccgccatttc caaacccaat gcttcaacca cgcttctgat gccgtctgtt
     5461 ttcgacgcac ccgcaggcag caaatcgacc gcttcctcgt gccagcgcac cgttttcaag
     5521 ccttcctgtt ccacaatatc cgaccagagc ggcatttcgt tttcctccgc aaacaccagc
     5581 atctgataca ccggtttgct tgaaaaataa tccttatcgg caaaaaaatc gctggcgata
     5641 tgctgcaagg cgcggcacac gcattccgac agcgcggaca cggcgatccc ctctccgccg
     5701 acaaacgcat aatccatgcc caagccatcc aaatgcgcgc aaaccctgcc catcaaaccg
     5761 gcatccatcg gtacttcgtg cacagttttt ccgtgcagca gcgcaaactg tccgtttatc
     5821 gttaccacgg catccatacc cgtttccgcc atcatgtccc tgaccttttc gggaatcgtc
     5881 gccaaagacc gccccgttgc caacgccgtc aatatacctt tgccgcacaa agccgccacc
     5941 gccgttttca cggaagggcg caaggtatcc gtatatttgc ggtacagcgt atcgtcaatg
     6001 tcgaaaaaca cgattttagg attcatcaca ttctctctcg cattcaaaca gccgcattgt
     6061 atcccaagca ggcaaatact tgataaatcc ttataaattt cccggcaaaa ttgaccgcga
     6121 acacgaaaag gcggataatc cgcccatcct caaacccttt tcagacggca tttgcagcaa
     6181 tgccgtctga aacattttta caaagcatac aaatcatgtt tcaacacaca ggacgacaca
     6241 taaagcgtcg ccctatatgt tgccctgatt cgaaaggggt tacgcccctc ccaaataaag
     6301 tctgattcta ccgccctaaa gggcggggtt tcaaccgaaa aggaaacacg atgaaagcac
     6361 ccgaactctt attgcccgcc ggcggattgg aaagaatgcg cgccgcctac gattacggcg
     6421 cagatgccgt ttacgccggc agcccgcgtt actccctgcg cgcccgcaac aacgaatttg
     6481 ccaaacttga tgttttagaa caaggcatta aagaagcgca cgatcgcaac aaaaaattct
     6541 ttttgaccgt caacaccctg ccgcacaatt ccaaactcaa aacctttgtt tccgatatgg
     6601 aacccttgat tgccatgaaa cccgacgcgc tgattatggc ggatccgggt ttgatcatga
     6661 ccgtgcgcga aaaatggccg gaaatgccga tccacctgtc cgtacaagcc aacaccacca
     6721 actactgggg cgtgaaattc tggcagaata tcggcgtcga acgcatcatc ctgtcgcgcg
     6781 aattgagtat ggaagaaatc gccgaaatcc gccaacaatg ccccgacatc gaactcgaag
     6841 tcttcattca cggcgcattg tgcatcgcct actccggccg ttgcctactc tccggctact
     6901 tcaaccaccg cgaccccaat caaggcacct gcaccaactc ctgccgttgg gactacaaag
     6961 tccacaacgc tacggaaagc gatgcgggcg atgcccagct tctgcaaggt ttcaactttg
     7021 aaaaagccca agaagaagcc aaccaaaact ttgaaggcat caacggtcaa aaacgccatc
     7081 cctacgccga caaggttttc ctgattgaag aatccaaccg tccgggcgag atgatgccga
     7141 ttatggaaga cgaacacggc acctacatca tgaactccaa agaccttcgt ggcattgaag
     7201 tggttgagaa actcgccaaa atcggcgtgg acagcctcaa agtcgaaggc cgcaccaagt
     7261 cgctctatta tgtcgcacgc gtggctcaat cctaccgcaa agcgattgac gatgccgtcg
     7321 caggccgtcc gtttgattac agcctgttga gcgaactcga aggccttgcc aaccgcggct
     7381 acaccagcgg cttcctcgaa cgccaccaca ctcaggatta tcaaaactac ctgaccggcc
     7441 attccaccgc caaacaaagc caatacgtcg gacacgttac cgaaatcgac aaaaacggct
     7501 gggcaacagt ggaagtcaaa aaccgcttcg ccgtcggcga ttcactcgaa atcatccacc
     7561 cgggcggcaa ccaaaccatc aaattggaac aaatgacccg caaaggccag cctgtcgatg
     7621 tcgcacccgg caacggcatt caggtcaaaa tccccaatat gcaaggtaaa gaaaaagccc
     7681 tcatcgcacg cgtattgaac ccctaagcca cgatgccgtc cgaaccccgt tttcagacgg
     7741 cattttgttg aatcttacgg tacaatggca atgatttccc accgcaagca aggatatccg
     7801 acatgaaaaa aaatcctcat actctccgcc gccgccctga tgccggcagt ctgccaatcc
     7861 ggcagcgcac ctaaaaacgc gcacacggca tccgccggca caccggccgg agaatcatgc
     7921 aaagtcatcc gggacgacac cgcaggaagc ggcaaaaaca tcatttaccg ttgcagcaac
     7981 ggtcaggcgg caataaatgc agccattgca gacggcgtat tggaatcagg gattcccgtc
     8041 agcttcggcg gcgggggcgt accggttgca aacggcagga atctggttac acggcaagca
     8101 gcaaacggcg ttggcaaatc cgactccgag gcctgcgaac acgccctaat caatgccgcc
     8161 cgcaaattcc agcagaccgc aggcaaactc ggcgggcgca gtgttaccgg cttccacagc
     8221 tacttcgaca agcagccgct gcaaggcggg caatacgact gtcagacagg ttcgttccac
     8281 gtccgcgtgg tcatgcgcgc caatgtcgtc cgttaacata tcggcaatca aaaaattagg
     8341 ctgtgtttta gtaatctgtt gatttcaatt atttgcaaag gaaaagacaa ttattttttc
     8401 ggttaggaag agacctatcc tattgaatac atttaagtta aatacgtcta tcaacactat
     8461 attatagtgg attaacaaaa atcaggacaa ggcgacgaag tcgcagacag taca
//
LOCUS       DAWKST010000083         8514 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.083, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000083 DAWKST010000000
VERSION     DAWKST010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.083"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(142..1338)
                     /locus_tag="WJL87_001294"
     CDS             complement(142..1338)
                     /locus_tag="WJL87_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ7799719.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPNHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKDLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            1689..1988
                     /locus_tag="WJL87_001295"
     CDS             1689..1988
                     /locus_tag="WJL87_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799720.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGSLRNEYLTVSGQTFVLNDLTGNM"
     gene            1988..2173
                     /gene="ccoS"
                     /locus_tag="WJL87_001296"
     CDS             1988..2173
                     /gene="ccoS"
                     /locus_tag="WJL87_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ7799721.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            2163..3473
                     /locus_tag="WJL87_001297"
     CDS             2163..3473
                     /locus_tag="WJL87_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7799722.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLSETVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            3629..4363
                     /gene="dnaQ"
                     /locus_tag="WJL87_001298"
     CDS             3629..4363
                     /gene="dnaQ"
                     /locus_tag="WJL87_001298"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ7799723.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIQVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGAAAEEKMETKPVVHTETKRSGKLKVIRADENELAEHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            4360..5049
                     /locus_tag="WJL87_001299"
     CDS             4360..5049
                     /locus_tag="WJL87_001299"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ7799724.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTLGIFER
                     HEGVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAIPVADTLKRADGGNISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGVRPLLIQGDVRNLKLTQPQD
                     AYIVRLLLDAV"
     gene            5081..5563
                     /locus_tag="WJL87_001300"
     CDS             5081..5563
                     /locus_tag="WJL87_001300"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ7799725.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRALLRDAYRSVQAKGWHVVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            5640..6311
                     /gene="rpiA"
                     /locus_tag="WJL87_001301"
     CDS             5640..6311
                     /gene="rpiA"
                     /locus_tag="WJL87_001301"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ7799726.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFICIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDKPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            6380..7051
                     /locus_tag="WJL87_001302"
     CDS             6380..7051
                     /locus_tag="WJL87_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ7799727.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDVNGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGRGNKDWMDAVGE
                     WLGIW"
     gene            7230..7976
                     /locus_tag="WJL87_001303"
     CDS             7230..7976
                     /locus_tag="WJL87_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ7799728.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
BASE COUNT         1898 a         2075 c         2508 g         2033 t
ORIGIN      
        1 tttaaattta atccactata aggttcaggt aagcggctgc aaccgtcaat gtctgaaaag
       61 aggcagtgct gtttaagcat ggaaagccgg ataaaaaaaa ctgcctccgt ttccggaggc
      121 agttcgtgtg cggataaaac cttacagacc gctcaaacgg gcagtgtcgt gggcaatcat
      181 cagctcttcg ttggtcggaa tgaccacggc aacggctttg ctgtcggcag tggtaatcac
      241 gccggcgttg ccgaagcggg ctttcaggtt ggcttcttgg tcgatgttca gaccgaggaa
      301 gcccaagtag ccgatcacgc gttcgcggat gatgtcggag ttttcgccga tgccgccggt
      361 aaagaccagt gcgtccaaac cgcctgcggc aaccgccata ctgccgatgt atttggcaag
      421 gcggtagata aacatatcca aggccaattt cgcgccttta tgccccttgg cggcttcttc
      481 ttcaatggtg cggcagtcgt tggacaggcc ggaaatgccg agcagaccgg attttttgtt
      541 cagcatttca gtgatttggg cgatggtcat attggcgttt tcggcgagaa agccgaatac
      601 ggaaggatcg atgtcgccgc tgcgcgtacc cattaccagc ccttccagcg gggtcaggcc
      661 catactggtg tcgcgcgatt cgccgttggc gacggcggta atggacgcgc cgttgcccaa
      721 gtgggcaatg accatacgca ggtctttttt gtctttgccg aggaagcgcg cggtttcgtc
      781 ggcgacgaag cggtagctgg taccgtgcgc gccgtaacgg cgcaggccgt atttttcata
      841 caactcctgc ggaacggcgt atttgtaggc gacttcgggc atggtttggt ggaaggaggt
      901 atcgaatacg acgacgttgg gcaggtcttt gaaaatgctt tgcgcggcac gcaggcccaa
      961 gaggtgggcg gggttgtgca ggggggcgag cgggatgcat ttttcgatgc cggcaatgac
     1021 ttcgtcgtca acgaggatgg attcgctgta cagttcgccg ccgctgacga cgcggtggcc
     1081 gatggcgccg atgcggctgt cgaggccgtg ggctttgagt tcttccatca gggcttcgac
     1141 cgcgccggtg tggttgggat gtgcggacag atcgactttg tgtttttcgc cgtttacttt
     1201 gaatgtgatg taggcatcgg gcaggttgag tttttcggca aggcagctga gcaggacttc
     1261 gccgctgccg ttatccagga ccgcgccttt gagggacgag ctgccgcagt tcaaaaccaa
     1321 gatcaatttt tgggacattt tcttactccg gaaagtttca gacggcattg gaatcggaca
     1381 cggatactaa ccggattcgt gccgaatccg ttttgccttc ctgggcggga aagtagtggg
     1441 gccgtctgaa acggttgata aaagaagcag gttattctaa caaaaaatct ttgcaattgc
     1501 ttggcttaat cgggcgtttg cgtgaaaatg gcggaagtcg ggtattttga cggaaaaaag
     1561 ggggatatgc ggtttgccgg tgttttcggc tgggggaggg agaatttctt tgcattcttc
     1621 ccgtccaaac tggtacattc gccctgtttt tagttggtgt cgtctgaaaa aaggcgggtg
     1681 ggaaatttat gcattatttc agtattcatg accaatcggg acggcatata ggctttttca
     1741 tcctgcttgc cgacgacgaa tcggaagcac ggccgcaaag cgggcgtttt gccgtcaaac
     1801 ttgaaggcgg aatggaaaat catgtcctgt cgccgttcgg tcagacggaa atcccccaat
     1861 attggcgcgt ggtcaaagac cgtatcgagc tgttttttga cgaagctccc gtcggaagtt
     1921 tgcgtaacga atatctgacc gtgtcggggc agacttttgt tttgaacgat ttgacaggga
     1981 atatgtgatg gaaagcatgt ttatattggt gcccatcagc attattttgg cgtttgtcat
     2041 cggctggttt ttttggtggt cgggcaaaaa cgggcagttt gacgacttgg aagggccggc
     2101 gcaccgcatc ctgatggatg atgattcgac aagtaaactg attgaaaaag agagggaaaa
     2161 cgatgtacgc taaaaaaggc ggtttgggac tggttaaaag ccgccgtttc gcacctcttt
     2221 tcgctacgca gtttctcggc gcgttcaacg acaatgtgtt caaaaccgcg ctgtttgtga
     2281 tgattgggtt ttacggtttg gggcaaaacg gcttcctgcc tgccggacag atgttgaact
     2341 tgggcgcgtt gctgtttatt ttgccgtatt tcctgttttc ctcgctgtcg gggcagttgg
     2401 gtaacaaatt cgacaaggcc gttttggcgc gttgggtcaa ggtgctggaa atgatcatta
     2461 tggcggtggc ggcatacggg ttttatatcc ggtctgcccc gctgcttttg gcgtgtctgt
     2521 tttgcatggg cgcgcaatcg acgctgttcg ggccgctgaa atacgccatc ctgcccgatt
     2581 atctcgacga caaagagttg atgatgggca acagcctgat tgaatcgggt acgtttgtcg
     2641 ccatcctgtt cggtcagatt ttgggaacgg cggtggcagg tgtaccgcct tatattgtcg
     2701 ggatactggt tttgctggtc gccgtcggag gcacggtcgg cagcctgttt atgccgtctg
     2761 tacccgccaa ggctgccgat acacaaattg agtggaatat tgtccgtggc acaaaatccc
     2821 tgctgagtga aacggtgcgg cacaagcccg tttttaccgc cattatcggt atttcgtggt
     2881 tttggtttgt cggcgcggtt tataccacgc aactgccgac ctttacccaa atccatctgg
     2941 gcggcaacga caatgttttc aacctgatgc ttgccctgtt ttccatcggt attgccgccg
     3001 gttcggtact gtgtgccaag ttcagcaggg aacggctgat gttggcttgg gtaacggttg
     3061 gtgcgttggg tttgacggtt tgcggcctgg ttttggtgtg gctgacgcac ggacaccgtt
     3121 ttgaagggct gaacggcatt ttttggtttt tatcgcaagg atgggcatat cccgtgatgg
     3181 cggtgatgac gctgatcggc tttttcggcg gatttttctc cgttccgctc tatacttggc
     3241 tgcaaaccgc cagtagcgag actttccgcg cccgcgccgt tgccgccaac aatatcgtta
     3301 acggtatttt tatggtttcc gccgccgttt tgagcgcggt gttgctgttt ttgtttgaca
     3361 gcatttcctt gttgtatctg attgtcgctt tgggcaatat tccgttgtcg gtatttttga
     3421 ttaagcgcga aaggcggttt ttaggcgcgg cggcaatcag gaaaaaacct tgaatgccgt
     3481 ctgaaaaaat tgtccccaaa atacacaatc cgcctgccca atgttgtgaa cacactgtga
     3541 ataagtgggt ttgtgcattg aaatgaaaac agaaaaatac attgcttaaa aaataagcaa
     3601 tttacgaaga tagaagaaag acaccgatat gacgacacgc caaatcatcc tcgataccga
     3661 aaccaccggt ctgtatgccg acggcggcga ccgcttggtc gagtttgccg gtttggaaat
     3721 ggtcaaccgc caaatgaccg acaaaaacct gcacctctat gtccaccccg agcgcgatat
     3781 gcccgaagag gcggcacgcg tccacggctt gacgatacag gttttggaag ggaaaaacgc
     3841 gccgccgttt gcagaggtcg gcaggcagat tgccgatttc ctgcgcggtg cggaactgat
     3901 tatccacaat gccaaattcg acgtgggctt cctcaatatg gagttccgcc ggatggggtt
     3961 gccgaccgtt gaggaactcg gctgtaccgt taccgatacc ctggcgatgg cgcgcgaaat
     4021 gttccccggg cagaaagcca gcctggatgc cttgtgcaac cgtttttccg tcgaccgcag
     4081 caagcgcgtc ctgcacggcg cattgatcga ctgcgaactt ttgggcgaag tctatcttgc
     4141 tatgacgcgg cggcagttcg acctgatggg tgcggccgca gaggaaaaaa tggaaaccaa
     4201 acccgtggtg cataccgaaa ccaaacgcag cggcaaactg aaagtcatca gggcggacga
     4261 aaacgagttg gcggaacacg ggcaatattt ggacggcttg ggcgaagcct gtatctggcg
     4321 caaggaagcc gtgccgtctg aaaacggcgg gacggacgca tgaagcgcaa aaatatcgcg
     4381 ctgattcccg ccgccggcat cggggcgcgt ttcggtgcgg acaaacccaa gcaatatgtc
     4441 gaaatcggaa gcaaaaccgt tttagaacat acgcttggga tttttgaacg gcatgagggc
     4501 gtcgatttga ccgtcgttgt cgtctcgccc gaagacacgt ttgccgataa ggttcagacg
     4561 gcatttccac aggttcgggt gtggaaaaac ggcggacaga cccgcgccga aaccgtccgc
     4621 aacggtgtgg caaaattgtt ggaaaccggt ttggcggcgg aaaccgacaa tattctggta
     4681 cacgatgccg cgcgttgctg cctgccgtct gaagctttga cgcggttgat agaacaggcg
     4741 ggcaacgctg ccgaaggtgg gattttggca attcccgttg ccgatacgct caagcgcgcg
     4801 gacggtggga acattagtgc aaccgtcgag cggacgagcc tttggcaggc gcaaacgccg
     4861 cagcttttcc gcgccgggct gctgcaccgc gcattggctg cggaaaactt ggacggcatt
     4921 accgatgaag cgtccgccgt ggaaaaattg ggtgtgcgtc cgctactgat acagggcgac
     4981 gtgcgcaatt tgaaactgac gcagccgcag gacgcataca tcgtcaggct gctgctcgat
     5041 gccgtctgaa cttcaattca aaacatcagg aacacaaacg atgacgaaca tccgtatcgg
     5101 acagggctac gatgtccacc aactgaccga aggcagaaag ctgatacttg gcggagttga
     5161 aatcccattt gaaaaagggc tgctcggaca ctccgatgcc gatgcgctgc tgcacgccgt
     5221 taccgacgcg ctgctcggtg cggcaggttt gggcgatatc ggcagccatt tccccgacac
     5281 cgccgcagag ttcaaagatg cggacagccg cgccctgctg cgcgatgcct atcggagcgt
     5341 tcaagctaag gggtggcatg tcgtcaatgt cgatacgacc gtgattgcac agaaacccaa
     5401 actcgcgccg cacattccgc aaatgcgtgc caacatcgcc gccgatttgg gcatagacat
     5461 ttcgtgtgtc aacatcaaag gtaaaaccaa cgaaaaactc ggttatctcg ggcgcatgga
     5521 aggcatagag gcgcaggcgg cggtattgct ggtacggatt tgaacgcggc gggcaggacg
     5581 tgcaaataaa tgttaagatg gcggctgttt gttgatacgg tttcccaaag gacaaaaata
     5641 tgacgacaca agacgaactc aagcgcatcg ctgccgaaaa agcagtcgaa ttcgtacccg
     5701 aaaacgaata catcggcatc ggtaccggtt cgaccatcaa ctttttcatc gaagccttgg
     5761 gtaaaagcgg taaaaaaatc aaaggcgcgg tatctacttc caaaaaatcc agcgaactgc
     5821 ttgcccagta cgacattccc gtcgtttccc tgaacgaagt atcgggtttg gcggtctata
     5881 tcgacggtgc ggacgaagtg aaccacgccc tgcaaatgat taagggcggc ggcggcgcgc
     5941 acctcaacga aaaaatcgtc gccagcgcat cagaaaaatt catctgcatt gcggatgaaa
     6001 gcaaatatgt ttcacgtttg ggcaaattcc ctctgcccgt ggaagtcgtc gaaagcgcgc
     6061 gttcccttgt ttcgcgcaaa ctgctcgcta tgggcggaca gcccgaactg cgtatcggct
     6121 acaccacgtt ttacggcaac caaatcgtcg atgtccacgg cttgaatatc gacaaaccgc
     6181 tgacgatgga agacgaaatc aacaaaatca ccggcgtact cgaaaacgga atattcgccc
     6241 gcgatgccgc cgatgtgttg attttgggga cggaagaggg tgcgaaagtc atctatccct
     6301 gccaagggta atcctgacgt taagatgccg tctgaaatcc gattccgata tgttcagacg
     6361 gcatcggcat ttgaaaaata tgcagattaa aaaaatcatg aaatggcttc ccgtcgcctt
     6421 gtcgcttttg ggtgcgttgg gttatacggg gtacggcagc gaggcggtgc ggacggcggt
     6481 tgccgtactc gacgtactcg gcgcggcagg ggacgcgggt tccgacgcgc ccgcccgccg
     6541 ccgagcatcg gcgaaatccg gtcaccgcta cacaggcacg gtgtccaaag tctatgacgg
     6601 cgacaccctg cacgttatcg atgttaacgg cgcaaagcac aaaatccgta tggcgtatat
     6661 cgatgcgccg gagatgaaac aggcttacgg cacgcgttcg cgcgacaacc tgcgcgcggc
     6721 ggcggaaggt aggaaagtca gcgtgcgcgt gttcgatacc gaccgctacc agcgtgaagt
     6781 ggcgcaggtt tctgtcggca aaaccgattt gaacctgatg caggtgcagg acggggcggc
     6841 gtggcattat aaaagttatg ctaaagaaca gcaggataag gcggattttg ccgattatgc
     6901 cgacgctcaa attcaggcgg aaagggaacg caaaggcttg tggaaagcta aaaatccgca
     6961 agcgccgtgg gcgtaccgcc gggcaggcag gagcggcagg ggcaataagg attggatgga
     7021 tgccgtgggc gaatggttgg gcatttggta aagacaccgc acggctttga acggcaaatg
     7081 ccgtctgaaa accgcgcgtc cggcattttt gcggagacgc gcaaccgccg tctgccgcga
     7141 taaaaaagtt caggcggcgg tttttacggt aaaatggcgg attattttgc aacggcggca
     7201 gttccgccgg tttttgatac ggacggttta tggattttcg ttttgacatt atttacgaat
     7261 accgctggat gtttctttac ggcgcactga cgaccttggg gctgacggtc gtggcgacgg
     7321 cgggcggttc ggtattgggt ctgttgttgg cgttggcgcg cctgattcac ttggaaaaag
     7381 ccggtgcgcc gatgcgcgtg ttggcgtggg cgttgcgtaa ggtttcgctg ctgtatgtta
     7441 cgctgttccg gggtacgccg ctgtttgtgc agattgtgat ttgggcgtat gtgtggtttc
     7501 cgtttttcgt ccatccttca gacggcattt tggtcagcgg cgaggcggca atcgcgctgc
     7561 gtcgcggata cgggccgctg attgccggtt ctttggcact gatcgccaac tcgggggcgt
     7621 atatctgtga gattttccgc gcgggcatcc agtctataga caaaggacag atggaggcgg
     7681 cgcgttcttt ggggctgacc tatccgcagg cgatgcgcta tgtgattctg ccgcaggcat
     7741 tgcgccgcat gctgccgcct ttggcgagcg agttcatcac gctcttgaaa gacagctcgc
     7801 tgctgtcggt cattgctgtg gcggagttgg cgtatgttca gaatacgatt acgggccggt
     7861 attcggttta tgaagaaccg ctttacaccg tagccctgat ttatctgttg atgacgactt
     7921 tcttaggctg gatattcctg cgtttggaaa aacgttacaa tccgcaacac cgctgattgg
     7981 agtcatatca acaaaaagct gcccgggtat tcatttgagg cagctttttt gtggcaaatt
     8041 tatcgtggat taacaaaaac cagtacggcg ttgcctcgcc ttagctcaaa gagaacgatt
     8101 ctctaaggtg gctgaagcac caagtgaatc ggttccgtac tgtttgtact gtctgtggct
     8161 tcgtcgcctt gtcctgattt ttgttaatcc actataaatt caaagagaaa acgccctatg
     8221 ccgtctgaaa gtctttcaga cggcatctgt cagcctcacg caagagggaa tcccatccat
     8281 acggaaacct gcaccacgtc attcctacga acctacatcc tgtcattccc acgaaagtgg
     8341 gaatccagaa cgtaaaatct gaagaaaccg ttttatccga taagtttccg caccgacaga
     8401 cctagattcc cgcctgcgcg ggaatgacgg cggagaggtt tcggtttttc cgataaattc
     8461 ccgtggcttt gggtttccgg attcccgctt tcgtgggaat gacgaatcca tcca
//
LOCUS       DAWKST010000084         8273 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.084, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000084 DAWKST010000000
VERSION     DAWKST010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.084"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(59..1315)
                     /locus_tag="WJL87_001304"
     CDS             complement(59..1315)
                     /locus_tag="WJL87_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ7799729.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPSDAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEETEFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWNEEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCAELLQSLKIDL
                     DTGKYLRLDSAKHQTDGFFAAVLQRK"
     gene            complement(1351..2208)
                     /locus_tag="WJL87_001305"
     CDS             complement(1351..2208)
                     /locus_tag="WJL87_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ7799730.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADPAPLTEHLTRRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGGGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPQSFHAFEPPLNPYGWRGIYIQD
                     WAEYGFERQEVRYHLLDRMAYLAPARAAETETLNETEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(2269..3084)
                     /locus_tag="WJL87_001306"
     CDS             complement(2269..3084)
                     /locus_tag="WJL87_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ7799731.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            3148..3318
                     /locus_tag="WJL87_001307"
     CDS             3148..3318
                     /locus_tag="WJL87_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799732.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVGRYFTRFGRIYPNQINSDFAAP
                     KDCASEPAVCHP"
     gene            3328..4113
                     /locus_tag="WJL87_001308"
     CDS             3328..4113
                     /locus_tag="WJL87_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ7799733.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMTGEDGWLESGDIVAANPKVLAQMLKIISAHV"
     gene            4383..6506
                     /locus_tag="WJL87_001309"
     CDS             4383..6506
                     /locus_tag="WJL87_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ7799734.1"
                     /translation="MGKANAFTAPITVVNYDEQALNNTEARTLVDAVAKKDASVWQFG
                     GESNTLTGLYFRGYQLDARQFSVNGLAGMYGTQGTASVQVGSAQLIKGASTAVNGMNP
                     EGAVSGSVNIETKKAADEGNRKIGLGRFSNNRAQGTFDLGQRFGENKAFGVRANGKLR
                     HGDTPRHGYSEDNKEFAVNADYRGETLRVAFDSIYAKRKTNGGRARMQDIQNAGGRLF
                     GAPDGKTNLLPSWNWQNTAGQTNMLTFEWDAFDNAQITGGIGYNKARYYGTLISPTVC
                     LNATSTCTDMQNTNASGKVTARNYDYNTGTARLTDQYFRTLSMNLTARGEFETSPVTH
                     NWSTAFDRVIRQRKTIRGTAAGAGKVEVKANENIANQLASFKADYPNSWENSANLDAN
                     IKVNSLALSDTLGFVDNKYRLTLGGRFQAVEYTNKKKSQSGDAKRFSPMLMAAWVPQP
                     DLVVYGNYMEDLEPADIKTDDSGETTMAKPRVSRQFEVGVRKNWGNFVTTLNAFQIKR
                     PGYWRGHTTTKTTKGVTTTLTYGNNSDFARYKAQGGAAGDEQGMERNRGIEFNTYANL
                     LNNTLRPTLGLMYLQSTVKEYPNSRDMLVNGVQVANPRVIAKAGLEWDTPFAKGLTLN
                     SNVSYFGKSYQDTQKQYAFPSYTLVDVGARYKTKLGKNTLTVSSSVENLFNKNYWQVQ
                     RGQYDRSFAVVGMPRTYWLKAELDF"
     gene            6630..8180
                     /locus_tag="WJL87_001310"
     CDS             6630..8180
                     /locus_tag="WJL87_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ7799735.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQETGFL
                     TVESHQYERGWFTSMETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQAPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGSFINSEGQFRFDTLVYGNEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTQPSGKIDVGGKIMFK
                     DMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEA
                     GMVSEPQQ"
BASE COUNT         2006 a         2030 c         2307 g         1930 t
ORIGIN      
        1 atgacatgcc gtctgaacgc tttgaaaagg gttcagacgg cattttgttt aaacagggtt
       61 atttgcgttg caatacggcg gcgaagaagc cgtcggtttg gtgttttgcc gaatcgaggc
      121 gcaggtattt gccggtatcc aaatcgattt tcaggctttg cagaagttcg gcgcagttga
      181 cgggttcaaa ttcgggatgt tcggacagga aacgttcgac ttgcagctcg ttttcttcgg
      241 gcaggatgct gcaagtggcg taaaccaagc ggccttgcgg ttttaccagt ttggaggcag
      301 catcgaggat gctgtgttgc tgttccaaaa ggttggccac ggtctcggca gattggcggt
      361 atttgaggtc gggattgcgg cgtaaagtac ccaaaccgga gcagggcgcg tccaccaaca
      421 cacggtcggc tttgcctgcc agtcgggcga tacggctgtc gtgttcgctg ctgatgcgtt
      481 cggggtggat attggtcagt ccggcgcggg tcatacgcgg tttgaggttg gcaaggcgtt
      541 tttcggcgat gtcgaaggcg tagattctgc ctttattggc catttgcgca ccgacagcca
      601 aggttttgcc gccggcaccg gcacagaaat cgacaatgat ttcgccgcgt tttgcgccca
      661 ccaataaagc aagcagctgg ctgccttcgt cttggacttc cagtgtgccg tctaaaaaca
      721 gttcgtgttt gttaagcgcg attttgtttt tcaggcggat gccccaaggc gaataaggcg
      781 ttgcctctgc atcggcactt tcggcttgca acagcggcag cactttatcg cgtttgcctt
      841 tcaaagtgtt gacgcggatg tcgagcggcg cggcttggtt gatgctgcgt ccgaaggcga
      901 ggatttcttc ttcgttccaa tgctgttgca gttgttcgac cagccattgc ggcaattctg
      961 cggcggtatt cagaccgtct gaaaactcgg ttttgcgggc tttcaaattg ccgaggaact
     1021 ctgtttcttc ttcatcaagc aggtctttga tttggctgat gttggtgctt ctgccgagaa
     1081 ccagtgcggc gagtgcggct ttgcgtggct gcgcgtgagg acggcgcagg gcagtgctga
     1141 ttttttgata gtggcgcagc gcggcaaagg cggtttcggc gatttcgtgg cggtcttggc
     1201 ggccgagctt tttatgttcg cggaagtagg cggagaggac ggcatcggaa ggctgtttga
     1261 aagtcagcat ttcagccaaa actttggcgg tatggtcgag ttgtgcggcg ttcataatgt
     1321 aatgggttct cttaaagttg gcaaagggtt tcagacggcc ttcataatgc gttcgatttc
     1381 gtgccaaaag ccgtccggcc gacattccaa aacggcaatc aagccttggt cgatacggcg
     1441 gatttcggtt tcgttcaatg tttcggtttc ggcggcacgc gcaggtgcga ggtaggccat
     1501 gcggtcgagc agatggtagc ggacttcttg gcgttcaaac ccgtattccg cccagtcttg
     1561 aatatagatg ccgcgccagc cgtatgggtt aagcggcggc tcaaaagcat ggaaactttg
     1621 tggaaaaaat ccgatgccgc gcacgatgga aacgggtttt acgtcaagcg gcagaccgtg
     1681 ttgccgcaag gttcggatgc cgtctgaagt gtgcggcaga ccgagttggg cggtcagttt
     1741 ggcggctttt cccaacagcg tgtctttggg gtcgagtccg cgcatgtctt cgagcctgcc
     1801 cgtgccgccg ccgtaatatt tgcaggtcag ctcgatatgg tagggcttgc cgttaagcct
     1861 taccacaaaa tccgccgcgc cttgcgtatt gccgtccgaa ccggaaaccg tgaggttgtg
     1921 cgcgagcagt tcggcgtgtg gcgcgttggc aaaccaaaat gccagcagtt cttcggcgta
     1981 aatgcccaag cggtgtccga acggggcgcg tcgggtgagg tgttccgtca gcggcgcggg
     2041 atcggcatcc aatgccaaaa gatagcggaa accgcgttcc cccaatagtt cgcgcacgcc
     2101 caattcgcaa ccgctttgcc acaaaggcgg cgcagtcagc agcgaggcga ggtcgcggac
     2161 gggttggctg gtgagcttcc accataatgc gtctagggcg taattcatgc ttatccgaat
     2221 atcagtcatg ccgtctgaaa agctggaaat gttttcagac ggccttaatc agtctttttt
     2281 atggatacga tggctgacgg tattgaaaaa accgcgcagg atatcgatgt cttcggtttg
     2341 cgtgttggcg cgtccgaaca ggctctgcat acggcgcatc agacgctcgc cgttgcggcg
     2401 gttgaaaaag ccgatgtcgt tcatcacgct ttccatgtgg gcgaccatgc ctttgatttg
     2461 ctcgtgggtc gcagcgtggt cttcttgttg aagatgggtc atgggcgaac cggtttggct
     2521 gaagatttcg tagcacacga cctgcacggc ttgggcgagg ttgagcgaga aatagtcggg
     2581 attgccgttg atggtcatca gtcggttgca ggcttggact tcttcgatgc tcaagccgaa
     2641 agtctcgttg ccgaaaacca gtgccacttt ctcgcctcgg tttgcggtct gcagtaattc
     2701 ggataccaaa tcgcgcgggg tttgcagcgg cgcagtaatt tcgcggcggc ggctggtcag
     2761 ggcgcaggcg atggtggtgt cggcaagggc ttcgtccaaa gaagcggcaa tggtggcatt
     2821 ttccaaaacg tctgccgcgc cggaagcgag gatgaagctt tcttccggta atttaaacga
     2881 ttgaggatgc tccgggtcaa acacgggcgg gttttccgtc atcggcgttg ccatcagatt
     2941 tggggcgacg atggtcagtt tgtgcagacc cattgttttc atcgcgcgcg cggcagagcc
     3001 gatgttggcg ggatggctgg tgcgcgtgag gatgatgcgg atgttgtcca gataagcggg
     3061 cagggcgggt ttgagagtag tcatgttcgg ttttcggtcg gaatggaata gggtataatg
     3121 cgccgtcttt ttcgttcgga cggtcaaatg ccgtctgaaa tgttctttaa caacgtcgga
     3181 aatttaacgt tgcgcctgaa gcagacacgc gtcgggcggt attttacccg atttggcagg
     3241 atttacccca accaaataaa ctccgatttt gccgctccga aggactgcgc gtccgaaccg
     3301 gcggtatgcc acccttaagg aaatctgatg aatccgtttt tgaatacagc ctttaaagcc
     3361 gcccgccgtg ccggtcagat gatgatacgc gccgcgggca atctcgatgc cgtcaaaacc
     3421 gacagcaaag cattcaacga ttttgtttcc gatgttgacc gcaattccga aatcatcctg
     3481 gttgaggctt tgaaagaagc ctatccgcac cacaaaatca cttgcgaaga aagcggctcc
     3541 cacggtaaag ccgcagccga atacgaatgg attatcgatc cgctcgacgg cacgaccaac
     3601 ttccttcacg gccatcccca atacgccatc tctatggcac tcctgcacaa aggcgtgttg
     3661 caagaagctt tggtgtacgc ccccgaacgc aacgacgttt acatggcttc gcgcggcaaa
     3721 ggcgcgttgc tcaatgaccg ccgcatccgc gtttccaacc gcatcgaatt gaaccgctgc
     3781 ctgatcggta ccggctttcc tgttgtcgat caaagcatga tggacaagta tctggcgatt
     3841 ttgaaagatt tcttggcaaa aaccgccggc ggccgtcgtg aaggcgcggc ttctttggat
     3901 ttgtgtgctg tggcgactgg tcgttttgac ggctttttcg agttcaacct caaaccttgg
     3961 gatattgccg caggcgcatt gattgttcag gaagcgggcg gtatcgtaac cgatatgacg
     4021 ggtgaagacg gctggctgga aagcggcgat attgttgccg ccaatcccaa agtgctggcg
     4081 caaatgttaa aaatcatttc cgcacacgtt taaatttcag cccgctcccg ggaatgcggg
     4141 aaaacgggcg ggtatgccgt ctgaagtatc gcgccgatat ttcagacggc atttttactg
     4201 gcagcggggg caggaaggtg gaaacggcta aatgcccgaa actgccgaag atggaaaccc
     4261 atatctgatt aaacggagaa ccctgtacga tttgtttata gtggattaac aaaaaccagt
     4321 acggtgttgc ctcgccttag ctcaaagaga acgattctct aaggtgctga agcaccaagt
     4381 gaatgggcaa agccaacgcc tttaccgcgc cgattaccgt cgtcaactac gacgaacaag
     4441 ccctcaacaa caccgaagcg cgtactttgg tggatgccgt agcgaaaaaa gacgcttccg
     4501 tttggcagtt cggcggagaa agcaacacgc tgaccggact gtatttcaga ggttatcagc
     4561 ttgatgcgcg ccaattcagc gtcaacggtt tggcaggtat gtacggcacg caaggcacgg
     4621 ccagcgtgca agtcggctcc gcacaactga ttaaaggcgc gtccaccgcc gtaaacggca
     4681 tgaaccctga aggtgcggta tccggttccg tcaatatcga aaccaaaaaa gccgccgatg
     4741 aaggcaaccg caaaatcggt ttgggccggt ttagcaacaa ccgcgcccaa ggtacgttcg
     4801 acttgggtca acgcttcggc gaaaacaaag cattcggcgt acgcgccaac ggcaaactgc
     4861 gccacggcga cactccgcgc cacggttaca gcgaagacaa caaagaattt gcggtaaatg
     4921 ccgactatcg cggcgaaaca ctgcgcgtag cgttcgattc catctacgca aaacgcaaaa
     4981 ccaacggcgg tcgcgcgcgt atgcaggaca ttcaaaacgc cggcggacgc ttgtttggcg
     5041 cgcccgatgg taaaaccaac ctgctgcctt cttggaactg gcaaaatacc gccggccaaa
     5101 ccaatatgct tacgtttgaa tgggatgcgt ttgataatgc acagattaca ggcggtatcg
     5161 gctacaacaa ggcacgctat tacggcacgc tgatttcccc gaccgtatgt ttgaatgcta
     5221 ccagcacttg taccgatatg caaaacacaa acgccagcgg caaagtaacg gcacgcaact
     5281 atgactacaa taccggtact gcgcgcctga ccgaccaata tttccgcaca ttgagtatga
     5341 acctgaccgc acgcggcgaa tttgaaacca gcccggtaac acataactgg agtacggctt
     5401 tcgaccgcgt tatccgccag cggaaaacca ttcgcggaac ggcagccggc gctggcaagg
     5461 tagaagtgaa ggcaaacgaa aatattgcga atcaattggc atcgttcaaa gccgactatc
     5521 ccaattcttg ggagaactct gccaacttgg atgccaatat caaagtcaac agcctggctt
     5581 tgtccgacac cttgggcttc gtggataaca aataccgcct gactttgggc ggacgtttcc
     5641 aagcggttga atacaccaat aagaaaaaat cacagagcgg cgatgccaaa cgcttcagcc
     5701 cgatgctgat ggcggcgtgg gtgccgcaac ctgatttggt tgtgtacggc aactatatgg
     5761 aagacttgga gcctgccgat atcaaaaccg atgattccgg cgaaacgaca atggcgaaac
     5821 cgcgtgtcag ccgtcagttt gaagtgggcg tgcgtaaaaa ctggggcaat ttcgtaacca
     5881 cgttaaacgc gttccaaatc aaacgcccgg gctactggcg cggtcataca actaccaaga
     5941 caacaaaagg ggtaacaaca acgctgacct atggcaacaa ttccgatttc gcccgctata
     6001 aggcgcaagg cggcgcggcc ggcgacgagc aaggtatgga acgcaatcgc ggtattgagt
     6061 tcaacaccta cgcgaacttg ctcaacaata ctttgcgtcc gactttgggt ctgatgtatc
     6121 tgcaatcgac cgtgaaagag tatccaaatt cacgcgatat gctggttaac ggcgtacaag
     6181 tcgccaatcc gcgcgtaatt gccaaggcag gtttggaatg ggatacaccg tttgccaaag
     6241 gcttgacttt aaacagcaat gtttcgtatt tcggcaagtc ttaccaagac acgcaaaaac
     6301 aatacgcttt cccgtcttac actttggttg acgtaggcgc gcgctacaaa accaagctcg
     6361 gcaaaaatac gttgaccgtc agcagctcgg tggaaaacct gttcaacaaa aactactggc
     6421 aggtacagcg cggccaatac gaccgcagct tcgccgtcgt cggtatgccg cgtacttact
     6481 ggttgaaagc ggaattggat ttctgatttc tccgcatccg gaaaatgccg tccgaacagg
     6541 tttcagacgg catttctttt aaagaaatat ggcgggatat gcttaaataa tgctattcta
     6601 gcgtctttga aacctgcttg tttgaagtga tgaaaaaacc tttgatttcg gttgcggcag
     6661 cattgctcgg cgttgctttg ggtacacctt attatttggg tgtcaaagcc gaagaaagcc
     6721 tgacgcagca gcaaaaaata ttgcaggaaa cgggcttctt gaccgtcgaa tcgcaccaat
     6781 atgagcgcgg ctggtttacc tctatggaaa cgacggtcat ccgtctgaaa cccgagttgc
     6841 tgaataatgc ccgaaaatac ctgccggata acctgaaaac agtgttggaa cagccggtta
     6901 cgctggttaa ccatatcacg cacggccctt ttgccggcgg atttggcacg caggcgcaca
     6961 ttgaaaccga gttcaaatac gcgcctgaaa cggaaaaagt tttggaacgc ttttttggga
     7021 aacaagctcc ggtttccctt gccaataccg tttattttaa cggcagcggt aaaatggaag
     7081 tcagtgttcc cgccttcgat tatgaagagc tgtcgggcat caggctgcat tgggaaggat
     7141 tgacgggaaa aacggtttat caaaaaggtt tcaaaagcta ccgcaacagc tatgatgcgc
     7201 ccttgttcaa aatcaagctg gcagacaaag gcgatgccgc gtttgaaaaa gcgcatttcg
     7261 attcggaaac ttcagacggt atcaatccgc ttgctttggg cagcagcaat ctgaccttgg
     7321 aaaaattctc cctagaatgg aaagagggtg tcgattacaa cgtcaagtta aacgaactgg
     7381 tcaatcttgt taccgatttg cagattggcg cgtttatcaa tcccaacggc agcatcgcac
     7441 cttccaaaat cgaagtcggc aaactggctt tttcaaccaa gaccggggaa tcaggctcat
     7501 ttatcaacag tgaagggcag ttccgttttg acacgctggt gtacggcaat gaaaaatacg
     7561 gcccgctgga catccatatc gccgccgaac atctcgatgc ttctgcctta accgtattga
     7621 aacgtaagtt tgcacaaatt tccgccaaaa aaatgaccga ggaacaaatc cgcaatgatt
     7681 tgattgccgc cgtcaaaggc gaggcttccg gactgttcac caacaatccc gtattggaca
     7741 ttaaaacttt tcgtttcaca cagccgtcgg gaaaaatcga tgtgggtgga aaaatcatgt
     7801 ttaaagacat gaagaaggaa gatttgaacc aattgggttt gatgctgaag aaaaccgagg
     7861 cagacatcag aatgagtatt cctcaaaaaa tgttggaaga cttggcggtc agtcaggcag
     7921 gcaatatttt cagcgtcaat gccgaagatg aggcggaagg cagggcaagt cttgacgaca
     7981 tcaacgagac cttgcgcctg atggtggaca gtacggttca gagtatggca agggaaaaat
     8041 atctgacttt gaacggcgac cagattgata ctgccatttc tctgaaaaac aatcagttga
     8101 aattgaacgg taaaacgttg caaaacgaac cggagccgga ttttgatgaa gccggtatgg
     8161 tttcagagcc gcagcagtaa tgggtcaaat gccgtctgaa atgttcaggc ggcattttgt
     8221 tgtattggaa actgccgttt tcaaccattc ctttagataa gccgtgtcgg att
//
LOCUS       DAWKST010000085         8247 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.085, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000085 DAWKST010000000
VERSION     DAWKST010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8247
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.085"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(148..1080)
                     /gene="cysK"
                     /locus_tag="WJL87_001311"
     CDS             complement(148..1080)
                     /gene="cysK"
                     /locus_tag="WJL87_001311"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ7799736.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1274..1879)
                     /locus_tag="WJL87_001312"
     CDS             complement(1274..1879)
                     /locus_tag="WJL87_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799737.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(1876..2727)
                     /gene="dapF"
                     /locus_tag="WJL87_001313"
     CDS             complement(1876..2727)
                     /gene="dapF"
                     /locus_tag="WJL87_001313"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ7799738.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPRFMPSEIPFVPELGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAEAVF
                     EGELAYS"
     gene            2864..3160
                     /locus_tag="WJL87_001314"
     CDS             2864..3160
                     /locus_tag="WJL87_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799739.1"
                     /translation="MFRILLLLFPCAILAACTRNIPANPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANRILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            3174..3842
                     /locus_tag="WJL87_001315"
     CDS             3174..3842
                     /locus_tag="WJL87_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ7799740.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(3914..6034)
                     /gene="pnp"
                     /locus_tag="WJL87_001316"
     CDS             complement(3914..6034)
                     /gene="pnp"
                     /locus_tag="WJL87_001316"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ7799741.1"
                     /translation="MFNKHVKTFQYGNQTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAINA
                     INEFADEVNPELWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWNA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTSV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6271..7134)
                     /locus_tag="WJL87_001317"
     CDS             complement(6271..7134)
                     /locus_tag="WJL87_001317"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ7799742.1"
                     /translation="MSEIGLVKIYFGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLVHIMPNHFTGQTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIQLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECERIIGKELAAEVRAKAIQLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPADQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPANVIQKTVEK
                     YQEALTLLTQD"
     gene            7335..8198
                     /gene="rfbD"
                     /locus_tag="WJL87_001318"
     CDS             7335..8198
                     /gene="rfbD"
                     /locus_tag="WJL87_001318"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ7799743.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGNAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLAAAIIRLLQQTSPVRGIYHYAGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
BASE COUNT         1974 a         2379 c         1909 g         1985 t
ORIGIN      
        1 tatttccctc aagcgatgtg gcggcaccaa cacctgatta aaacacaacc ggcgttttcg
       61 gcatatacgc aaaaacgcat tttccggcac ggcgttacac cgataccgaa aaatgcgtct
      121 gaatgtttcg acaatccgat taaagcatta tgccaaatct gcaaaaagtg gcgtagagag
      181 atagcgttcg ccataagaag gcagcagcac gactatcagc ttgccttcgt tttcaggctg
      241 tttggcaagc tgcaacgcac tccaaaccgc cgcaccggaa gaaataccca ccaaaatgcc
      301 ttctttttcc gctattgcgc gggcggtttc aaaagccgct tcgttcgaca ctttggtaat
      361 gctgtcgtaa attttggtat tcaaaacggt cggaataaag cctgcgccga tgccttgaat
      421 cgggtgcggg cctttttcgc cgccgctcaa tacgggtgaa gcttcaggct cgacggcaac
      481 cactttaact tcgggtttgt attttttcaa cacttcgccc acgccggtaa tcgtaccgcc
      541 cgtgccgacg ccggcaacga agacatcgac tttgccgtcc gtatcccgcc aaatttcctc
      601 ggcggttgtt ttgcggtgga cttcggggtt tgcctcattg tcgaactggc gcggcataaa
      661 ataagtgtcg ggatgcgcgt ccaccaagga tttcgctttg gcaatcgcgc ccgccatacc
      721 ttcggcggca ggggtcagaa tcagctccgc accaaacgcg cgcaacagca ttttgcgctc
      781 cttactcatg ctttccggca tggtaatcgc cagcttgtag ccgcgtgcgg cacataccat
      841 tgccaaaccg acacccgtat tgccgctggt tgcttcgaca atgacggtgt ttttgttgat
      901 tttgcccgct ttttcggcac cctcgatcat tgcttcggca atgcggtctt tgacgctgct
      961 gcctggattg aaaaattcca gtttcacggc aacctccgcc ttcaaacctt cggtcagacg
     1021 gttcagtttg accaaaggcg tgttgccgat tagttcggtg atgctgtttg caattttcat
     1081 cttctgctcc tgaataataa ggtttcgaca gtaaggcgca atccccatag gcatcctgtc
     1141 cgattgcagg ggatttgcgc cgaaacataa acaaatttcg gatatggctt tatcctaaac
     1201 ctatgccgcc taaaaaacca atatccaaaa tttctttctt tataactttt tgttccgata
     1261 cggccgcacc gtattatctt ccaatttctg aaagcagcag caccagtacc gagacctgca
     1321 tcaacgctgc cgtcaacccg ccgagtcgca gctgcgagcc gccgaacgtt gacggcggct
     1381 gccggtctat accgcagcgg tggacatagg caaccgccat cagcacaaac tgcaacacga
     1441 accaataaac cggcttcagc gcataaagtt tgagcagtgc cgcaaacaca aacacttgaa
     1501 ccgttcccga aaagcaataa tgcaccaacg cacatattcc gacaaaagca agcgtcatcg
     1561 atacattact gacggcaaac agcccgagca gcggatgttc ggggtctgcc tgccatccgt
     1621 ttccatccgt tttccggttc caatgcccga tgaaaaaaaa tatgctgccc aaagtcaggt
     1681 aaaaatgggg gatgaacaag ggcgcggcgc gggtcattcc ccatatgccg ggcatcagct
     1741 ttgcccccaa aaccgatatg cccagccaca gcataatact cgcccaaaac cattgcaaca
     1801 ttcctgcgcg gaatatcaga aacagaaaaa ccgccgcata cagtaaggca gacaaaaaat
     1861 ccaaatgcag caaaatcatg aatacgccaa ctcgccttca aacaccgctt ccgcagggcc
     1921 ggtcatcatc acatcgccgc cgcaggccca ttcgatatat aaagtcccgc ccggcaaaat
     1981 cacctctacc gttttccctt catccaacaa ccccagacgg atacccgcca ccacagccgc
     2041 acacgcgccc gtaccgcacg cttgggtttc gcccacgccg cgctcgaaca cgcgcaaacg
     2101 aatcgcggtt cggctgacaa tctgcataaa gccgacgttg acgcgttcgg gaaactgcct
     2161 gtgcggttcg ataagcgaac cggtttcgcg caccggcgcg cattctacgt catcgaccac
     2221 aatcaccgca tgggggttgc ccatattgac gcagctgaca ggctgaatgc cggattcgag
     2281 atgcaccccg taaatacagg catcatcccc ctcgcccaat tcggggacaa acggtatttc
     2341 agacggcata aacctcggtt tgcccatatt gaccgtaacc atgccgttat cggacaattt
     2401 cggaaaaata acgccatttg ccgtttcaac acaaatttct ttcttatcgg tcaaaccctt
     2461 gtctgcaaca aaacgggcaa aacaacgcgc tccgttgccg cattgcccga cctcgctgcc
     2521 gtcggcattg aaaatacggt aacggaaatc cacgccttcg gtttccgaac gcccgaccac
     2581 caaaagctgg tcgaagccca cgcctcggaa gcggtccgcc catgccgcaa tcggcgcgtc
     2641 ctcgggggta aaatcctgac tgaccgcgtc aatcaccata aaatcgttgc ccaaaccgtg
     2701 cattttggta aatttcaacg tgttcatatt gttccgaaat cccgtatcgt ctgttgcttc
     2761 cgaatccgaa atcccccgca ccgaagggaa atgcttccat ctcccgccgc ctgaggatta
     2821 aaatcagcca ttatactgtt tgttacccct tacaagaaac accatgttcc gcatattact
     2881 gttgcttttc ccttgtgcaa tccttgccgc ctgcacccga aacataccgg caaacccggc
     2941 cgtttcaaat tgcgcgcatc ttcaaaaccg caccatcctg tattttgacg cacacggcaa
     3001 acccgccgac ggcgcagtaa agggcggatt ttacaaaatt atcgaaatac agggtgcaaa
     3061 ccgcatcctt attcaagact ttaacagcac gggcaatgcc ctcggcggat cctatgtgcc
     3121 ggactgccgc aaatcatccc ttttcccgaa aaatatttga cttcaaactg cctatgaacc
     3181 acgacatcac tttcctcacc ctgttcctac tcggtttctt cggcggaacg cactgcatcg
     3241 gtatgtgcgg cggattaagc agcgcgtttg cgctccaact ccccccgcat atcaaccgct
     3301 tttggctgat cctgctgctt aacacaggac gggtaagcag ctatacggca atcggcctga
     3361 tactcggatt aatcggacag gtcggcgttt cactcgacca aacccgcgtc ctgcagaata
     3421 ttttatacac agccgccaac ctcctgctgc tctttttagg cttatacttg agcggtattt
     3481 cttccttggc ggcaaaaatc gagaaaatcg gcaaaccgat atggcggaac ctgaacccga
     3541 tactcaaccg gctgttaccc ataaaatcca tacccgcctg ccttgcggtc ggaatattat
     3601 ggggctggct gccgtgcgga ctggtttaca gcgcgtcgct ttacgcgctg ggaagcggta
     3661 gtgcggcaac gggcgggtta tatatgcttg cctttgcact gggtacgctg cccaatcttt
     3721 tggcaatcgg cattttttcc ctgcaactga aaaaaatcat gcaaaaccga tatatccgcc
     3781 tgtgtacggg attatccgta tcattatggg cattatggaa acttgccgtc ctgtggctgt
     3841 aagccccatt cattcccgat acccgtaaaa ataccgtctg aaacctgttc agacggcact
     3901 ttcaccctaa gcgttactcg gcggcatttt cctcacgggc aggcgcgtcc agcagggctt
     3961 tgatggacag acggacacgg cctctgtcgt ccacttccaa tgctttcacg ttcaccacct
     4021 gaccgacttg caggtagtcg ccgacattgc gtacgcgctc gtgggcgatt tggctgatgt
     4081 gtaccaaacc gtctttgccc ggcatcacgc tgacaatcgc gccgacattg ttgtcgagga
     4141 ttttcaccac agtgccttcg tacactttgc ccacttccac ttcggcagta atctgctcga
     4201 tgcgtttttt cgccgcatcg ccggcttctt gagtggttgc ggcaatggta atcgtaccgt
     4261 cttcggcaat attgatttcc gtaccggttt cagcggtaat cgaacggatg gtttcaccgc
     4321 ccttaccgat aacttcgcgg attttgtctt ggttgatttt catcgtgaac aagcgtggcg
     4381 cgtgtgcgga cagctcttgc gggcccgcaa cggcggcttt catctgatcc aagatgtgca
     4441 gacgcgcttc tttggcctgt gccaaagcga tttgcataat ttctttggta atgccttgga
     4501 ttttgatgtc catttgcagc gcggtaacgc cttcggtcgt accggccact ttaaagtcca
     4561 tatcgcccaa gtggtcttcg tcgcccaaaa tgtcggtcag gacggcaaat ttgttgcctt
     4621 ccagaatcag acccatcgcg ataccggcaa cgtgtgcttt caaaggcacg ccggcagaca
     4681 gcaggctcag gcagccgccg cagacggaag ccatagagga agagccgttg gattcggtaa
     4741 tttcggagac cacgcgcatg gtgtagctga aatcttcagg tttcggcaat acggccaaca
     4801 atgcacgttt agccaaacgg ccgtgaccga tttcacggcg tttcggtgcg cccatgcggc
     4861 ccacttcgcc ggtagagtac ggcggaaagt tgtagtgcag cataaagcgg tcggtgtatt
     4921 cgccggacag cgcgtcgatg atttgctcgt cgcgcgaagt acccaaagtt gcaacggcca
     4981 aagcttgggt ttcgccacgg gtaaacaatg cagaaccgtg cgtgcgcggc aatacgctgg
     5041 tttggatgtt cagcggacgg acggtgcggg tgtcgcggcc gtcgatgcgc ggttggccat
     5101 ccaaaatttg gctgcggacg acatcggctt ccaagtgttt gaaaatgcct ttgatttcgt
     5161 tggctgccaa agtgtcggtt tcttcggtaa tcaacgcttc tttcacggca ttccaagctt
     5221 cgtccaactt ggcagaacgc gcttgttttt gacggatttt gaacgcttct ttaatggttt
     5281 cgccggcaat cccgcggact ttggcaacca gttcctcatt ggtttcaggt gctttccaat
     5341 cccaaagttc cggattgact tcgtcggcaa attcattgat tgcattgatg gcaacctgca
     5401 tttgatcgtg gccgtaaacc accgcgccca gcatcacgtc ttcgggcagg attttggctt
     5461 cggattccac catcaacacg gcttttgaag taccggcgac caccaagtcc aattgcgatt
     5521 tagccaattc ggctttagtc gggttcaaaa cgtacacgcc gtttacataa cccacgcgtg
     5581 ccgcaccaat cggaccggca aacggtacgc cgctcaacac cagcgcggca gatgcgccca
     5641 acattgcagg aatgtcggaa tcgatttccg gatcgacaga caccaccatc gctacgattt
     5701 ggatgtcgtg gtagaaacct tcagggaata aaggacgaat cggacggtcg atcagacggc
     5761 tggtcaggat ttctttttcg ctttgtttgc cttcgcgttt gaagaagccg ccggggattt
     5821 tgcctgcggc gtaagtacgc tcgaggtaat cgacggtcag ggggaagaag tcttgacctt
     5881 ctttcacttc tttattggtg gtaacggcaa ccaaaacgac ggtgtcgccc atagatactt
     5941 taacggcagc ggcggcttgg cgggcgattt cgccggtttc caaagtaacg gtttgattac
     6001 cgtattggaa ggtcttaacg tgtttattga acattattgt tcctttcaaa ataccgcact
     6061 gctaaaacac taataatgca cactaaaatc cgaatgtgca tagttagggt ttcagaccgt
     6121 gcggcaggtt ataaacaagc tttcagacgg cactccggac gctgaaaaca gcccccgatt
     6181 ataaaggcaa ccatccttaa aatccagtat tgatacaata aaaaggccgt ctgaaccata
     6241 tttctttcag acggccttca aacttaaaaa tcaatcctga gtcagcaaag tcaatgcttc
     6301 ctgatatttt tcgactgttt tttgaatcac attggcaggc actttaggcg caggggcttt
     6361 tttgttccaa ccgctttgtt ccagccagtc gcggacaaat tgtttgtcaa aagacggcgg
     6421 atttgtgccg actttatatt ggtcggcagg ccagaagcgg ctcgaatcgg gggtcaatac
     6481 ctcatccatc agcgtcagcg taccgtttgt atccaaaccg aattcaaatt tggtatcgca
     6541 aatgatgatg ccgcgcgatt tggcatattc cgccgcttct gtataaagct gaatcgcttt
     6601 ggcgcgcact tccgccgcca attcttttcc gataatgcgt tcgcattctt caaagctgat
     6661 gttttcatcg tgatcgccga ctgcggcttt ggttgagggc gtaaaaatca cttcaggcag
     6721 ttgttgcgct tcctgcatac cttcaggcag ttgaataccg caaaccgagc cggttttttg
     6781 ataatctttc caaccgctgc ctgccagata accacgcaca atcgcctcta ctttcaccgg
     6841 agtgagcttt ttagccacga cggcgcgttt ctctaaagct ttggcttcgt tttcaggcaa
     6901 aacatcgtaa accgtttgac cggtaaagtg gttgggcata atatgcacca gttttttaaa
     6961 ccaaaaattg gaaatctgcg tcagaatctc ccctttgctc ggaatcgggt cgtccaaaat
     7021 cacatcgaat gcagacaggc ggtcggaagc gaccatcagc atacgtttat cgtcgatttc
     7081 atataaatcg cgcacttttc caaaatagat ctttaccaaa ccaatctcac tcatttcgcc
     7141 ccccccctga aaatatcttg aaaataccga cccgacaccc gacaggtttg aatcacaaac
     7201 caatattcta gccgaagtcg gcgcaaaaca atacccatgg cacaaaaaac caacccgtca
     7261 accgtcggca aagttttggc actataatac cgacagcaag tcctacaata cacttttacc
     7321 aaaggaaata cctcatgaga atcctattga caggctcaaa aagccaactg gcacgctgcc
     7381 tgcgcgaccg tcttccggaa gactgggaaa ccattgcgac ggattccgca tccctagaca
     7441 ttaccgatgc cgatgccgtc tgcaacatgg tcaaaagttt ccaacccgac gctattgtca
     7501 acacggctgc ctatactgcc gtcgacaaag cggaaggcaa tgcggcagcg gcatttgccg
     7561 tcaatgcttc cgccgtttac aaccttgcct tggcagcaca tcgcgcccat gcccgattca
     7621 tccacatctc aaccgactat atctttgacg gtaaagggaa aaggccctat caggaaagcg
     7681 actttaccaa tccttccaat gtatacggac aatccaaaac cgcaggcgag ctgctcgcac
     7741 tgtctgccaa tcccgacagc cttatcctgc ggacttcttg gctgtttagc gaatacgggg
     7801 acaactttgt ccgcaccatg ctgaaccttg cgcgggaacg ttccccgctg tccgccgtcc
     7861 acaaccaaat cggctgcccg acctatgccg gcgatttagc tgccgccatc atccgccttt
     7921 tgcagcaaac gtcgcccgtg cgcggcattt accactacgc cggcggcaaa tccgtatcct
     7981 ggtacgaatt tgcccaacat attttccaag cggcatcgca acagcagaca tccttccccg
     8041 ttcccgaatt gactgccgtt tcagacaagg aatatccgac cgccgccccc aggcccgcat
     8101 acagcatttt ggactgccgc aaaatcgaaa acgacttcgg catcaaaccg tcagactggc
     8161 aaaaagccct tgcacaggtc gtttccaagc tgctctgatg ccgcccgcct ctccgtttcc
     8221 gccgtcaagc accgccttgg cggtttt
//
LOCUS       DAWKST010000086         8234 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.086, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000086 DAWKST010000000
VERSION     DAWKST010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8234
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.086"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(208..1593)
                     /gene="pntB"
                     /locus_tag="WJL87_001319"
     CDS             complement(208..1593)
                     /gene="pntB"
                     /locus_tag="WJL87_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ7799744.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIAPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALGVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TSSSGSQEIGEYREVKAADVAEMLKGANSVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1659..2042)
                     /locus_tag="WJL87_001320"
     CDS             complement(1659..2042)
                     /locus_tag="WJL87_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ7799745.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRDFLAHTQ
                     GTAARAHAAQQNGFEAFAPFAAAVLTAHATGNAAQSTINTLACLFILFRLAFIWCYIA
                     DKAAMRSLMWAGGFACTVGLFVAAA"
     gene            complement(2046..3587)
                     /locus_tag="WJL87_001321"
     CDS             complement(2046..3587)
                     /locus_tag="WJL87_001321"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ7799746.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTHDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KPEPKPVSLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIAGINIFGGFAVTRRMLN
                     MFKKG"
     gene            3877..4710
                     /gene="serB"
                     /locus_tag="WJL87_001322"
     CDS             3877..4710
                     /gene="serB"
                     /locus_tag="WJL87_001322"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ7799747.1"
                     /translation="MPQALVLQFPSAEALPSDFPSRLPEPDCADEKRMRFIVEEGFSL
                     SGADAALLGSRQIDHAVLPDMDFGELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPMLKEAGIGVAYRAKPKAQAAADACINFGGLERVRGLF
                     G"
     gene            4774..5996
                     /locus_tag="WJL87_001323"
                     /pseudo
     CDS             4774..5996
                     /locus_tag="WJL87_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            6161..7741
                     /gene="purH"
                     /locus_tag="WJL87_001324"
     CDS             6161..7741
                     /gene="purH"
                     /locus_tag="WJL87_001324"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ7799748.1"
                     /translation="MPSIKRALISLSDKTGAVEFAQTLTKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRCDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGEPEIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYVTDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDIHR
                     LNRADLKVVSKRQPTEQEWNDLMFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDANLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTDVRHFRH"
BASE COUNT         1830 a         2259 c         2342 g         1803 t
ORIGIN      
        1 taaaaatcca tcttcaacac ggagatggat tttgtttgtt tcgttgattt tgtgtcagtt
       61 tcagatgtag gtgcttattc ggacgggccg tctgaaaatg tttgccccaa tgcaaaaaaa
      121 atcactgcaa accttcataa acggggtttg cagtgatttt ttcaaatcaa acagattgaa
      181 aacctgcgcc gaattgttca gacggcatta ttttttcagt tcggacagaa tgtcatccac
      241 ggttttcttc gcatctccga aacacattac gctgttttcg ttgaagaaca gcgggttttg
      301 tacacctgcg tagccggtat tcatcgagcg tttgaagacg acgacttctt ttgccttcca
      361 tacttccaac acgggcatac ccgcaatcgg gctgttcggg tcggtttggg cggcaggatt
      421 gacggtatcg ttcgcaccaa tcaccaagac cacatcggtt tcggggaagt cgtcgttgat
      481 ttcgtccatt tccaacacga tgtcgtaggg gactttggct tcggcaagca gtacgttcat
      541 atgaccgggc aggcggccgg cgacggggtg gatgccgaag cgcacttctg tgccgttttt
      601 acgcaaaagc tcggtgattt cggcaacggg gtattgcgct tgtgcgactg ccataccgta
      661 gcccggggta atgatgacac tgtttgcgcc tttcagcatt tcggcaacat cggcagcttt
      721 gacttctcgg tattccccta tctcttggct gccggaagat gatgtgccgc tgtcgctgcc
      781 gaaaccgccg gcgattaccg agacaaacga ccggttcatg gctttgcaca taatgtagga
      841 cagaatcgcg ccgcttgagc cgaccaacgc gccggtaacg atgagcaggt cgttggagag
      901 cataaagcct gccgctgcgg ccgcccagcc ggagtaggag ttgagcatgg acacgaccac
      961 gggcatatct gcgccgccga tggaggcaac caagtgccag ccgaatgcga gggcaatcag
     1021 ggtcataatc agcaggatga agccgctgcc gtcaatgccg acgaatacga gcagcaacac
     1081 aaacgatacg ccaagtgcca gtgcgttgag cttgtgtttg gcgggcagtt gcagcgggct
     1141 gctgctgatt ttgccgttga gtttgccgaa tgcgaccagc gagccggtaa aggttaccgc
     1201 gccgatgaag atgcctaaat acacttcgac cagatggatg gtgtgcatat cgtgcgaaac
     1261 gttgcccggc gcgatatagc tgttgaagcc gaccaaaacc gccgctaggc cgacgaagct
     1321 gtgcagcagg gcaatcagtt cgggcatttc ggtcatttcc acctttttgg ctttgtagat
     1381 gccgattgcc gcgccgatga gcatggcgat gatgatccag cccagtccgt gggtattgtc
     1441 ggaaaaaaca gttacaaaaa gggcgaccgc cataccggcg ataccggaat agcagccctg
     1501 tttggcggtt tcctgtttgg acagccccgc cagtgagaag atgaataaaa ttgcggcaac
     1561 gatatacgcc gctgttacga gtcctgaaga catggaaatt ctccgatttt cgatgatttg
     1621 ttttcaatgc cgtccgaact gttcagacgg tatctgtttc aagcagccgc gacaaacagc
     1681 ccgacggtgc aggcaaatcc gcctgcccac atcagtgagc gcatagcggc tttgtcggcg
     1741 atatagcacc agataaaggc gaggcggaac aggatgaaca ggcaggcaag cgtgttgatg
     1801 gtcgattgcg ccgcattgcc ggttgcgtgt gccgtcaaaa cggcggcggc aaacggtgca
     1861 aaggcttcaa aaccgttttg ctgcgcggcg tgggcacggg cggctgtgcc ttgcgtgtgc
     1921 gccagaaaat cgcgcggatt gtggttgtct ttaaaccgga atccgcccgc ttttttggca
     1981 tatgccgcac aaaaaagcgg caataggcag gcaatcagga tgcaccaata ggcgaaagtc
     2041 atggcttacc ctttcttaaa catattcagc atacgccgtg taaccgcaaa gccgccgaag
     2101 atgttgatgc cggcaatcag gatggcaaca aacgacagca gcgaaacaaa gccgttgccc
     2161 tgaccgattt gcagcagcgc gccgacgacg atgatgccgg agatggcgtt ggtcacggac
     2221 atcagcggcg tatgcagcga gtggctgaca ttccaaacga cgtagtaccc gatgacgcag
     2281 gcaaggacga acacgataaa gtggttcagg aatgctgcgg gtgcgaccgc gccgacccac
     2341 agcaccaata cggcggcgat gacggcgggc gcgagttttt tccacaggga aacaggtttc
     2401 ggctcgggct tggcggcagg cgcggctttt tcagacggcg tttgctgcgg ctgggcggaa
     2461 acttgaatcg gcggaggcgg gaaggtgatt tcgccgtcgt gggtaacggt catgttgcgg
     2521 ataatcacgt cttcgaagtc caacgtgatt tcgccgtctt tgttcgggct taacagcttg
     2581 gtcaggttga ccaagttggt ggcgtaaagc tgggaagact gtccggcaag gcggtttgcc
     2641 atgtcggtgt agccgatgat tttcacgccg ttgccggtta cggacaattc gcccgggcgg
     2701 gtgagttcgc agttgccgcc cgtcgccgcc gccaaatcga cgatgacgga gccggatttc
     2761 atgctttcca ccatttcttt ggtaatcagc ttgggcgcgg gtttgcccgg aatggcggcg
     2821 gtggtgatga tgatgtccac ttctttcgcc tgctcggcaa agagcttcat ctcggcggcg
     2881 ataaattcgt cgctcatcac tttggcgtag ccgtctccgc tgccgcccga ttcttgtggg
     2941 aagtcgagtt tcaggaactt gccgcccatc gattcgattt gttccgccac ttccaagcgg
     3001 gtatcgaacg cgcgtaccac tgcgcctagt gaatttgccg taccgattgc cgccaaacct
     3061 gccacacctg caccaatcac caaaacctgc gcaggcggca ctttgccggc ggcagtaatt
     3121 tgaccggtga agaaacggcc gaaggcgttg gcggcttcaa tcacggcgcg gtagccgctg
     3181 atgtttgcca tcgaagacaa agcgtccaaa gcctgcgcgc gggaaatacg gggcaccatg
     3241 tccatcgcca gcgcattgac tttcttggcg cgcaaggctt cgaccaaagc ctcgttttgg
     3301 cgcggccaca ggaagctgac gatggtttga ccttcgttca aaagcggcag ctcgccttcg
     3361 gacggcgcgt taaccttata aatcaaaggg caggcccaaa ccgccgcttt gtcggcaacg
     3421 gttgcgcctg ctgtttggta agcggcatcg tccaaacttg ccgccaaacc tgcaccgctt
     3481 tcgacaacgg tttcaaagcc cagtttgccc agcagggcga cggtggcggg cgtgcaggcg
     3541 acgcgggttt cgccggataa tgactcgcgt gggataccga ttttcatctc tgaatccttt
     3601 ttcgggtagt ttatatgtat cgtgggttaa atttaaatcg ggggggggcg gagcaacgcc
     3661 gtaccggttt aaagccgact cacttcaaat gttaatatat tttagataat ccccttataa
     3721 cgaattttca tcaggctggc aatagttgcg gcattttccc gtgttgtccg acacatactg
     3781 ccgtttgtgt ttgaagtcat tttccgattg caggcccgcc cgccgtaaca ttctttgccg
     3841 tttcactata taatccgcat tttttgagcc gccgttatgc cgcaagccct cgtcctccaa
     3901 tttccctccg ccgaagccct gccttccgac ttcccctccc gcctgcccga acctgattgc
     3961 gccgatgaaa agcgtatgcg ttttatcgtt gaagaagggt tttctttaag cggggcggac
     4021 gcggcgttgc ttggcagccg tcaaatcgac cacgccgtgt tgccggatat ggatttcggc
     4081 gaactcggtt tgattgtcag cgatatggat tcgacgctga ttaccatcga atgcgtcgat
     4141 gaaattgcgg caggcgtggg tttaaaagac aaggtagcgg aaattaccga gcgttcgatg
     4201 cgcggagagt tggatttcga gcagtcgttg cgcagccgcg ttgcgctgtt ggcgggattg
     4261 gacgaacaga ttttggcgga cgtttatgaa aacgttttga agctctcgcc cggtgcggaa
     4321 tttttgttgg acgaatgcaa aaggcacgat gtgaaattcc tgctggtgtc cggcggcttc
     4381 acgtttttta ccgaacgtct gcaacaacgc ctcggcttcg aataccaaca cgccaatgtt
     4441 ttggaaattg aaaacggcag gttgaccggc cgtctgaaag gcagaatcat cgacgcgcag
     4501 gcaaaggcgg atttattgcg cgaataccgc agccgcctcg gattgcagcc acatcaggtg
     4561 ttggcgatgg gcgacggtgc gaacgatatt ccgatgctca aagaagcggg cataggcgtg
     4621 gcttaccgtg ccaaaccgaa agcgcaggca gccgccgatg cctgtatcaa tttcggcggt
     4681 ttggagcgtg tacgcggcct gttcggatag gcggatagga aacggatgtc gtccgaaagg
     4741 ttttcagacg gcatttgaac ggcaggaacg acagtgggac gcagaaaact ttggtttgcc
     4801 ctggcagcag caggcgttat cggcggtttg gtcggcattg tgctgacgga actgatgcac
     4861 ttcatacagc atacggcaac ggttatggcg cggacggcgt gtacacttcg ttccgcgaag
     4921 gcgtggcaca ggcttccggt atgcggcgcg ttgccgtgct gacgctgtgc ggcgcggtcg
     4981 caggcagcgg ctggtggttg ctgaaacgtt tcggcaagcc gcaaatcgaa atcaaagccg
     5041 ccttgaaaca gccgttgcag gggctgccgt ttctgacgac ggttttccat gttctgctgc
     5101 aaatcataac ggtcggactc ggttcgccgc tcggacgcga agtcgcgccg cgagaaatga
     5161 ccgccgcgtt tgcttcggca ggcggcaaac gcttgggttt ggacgaaggc gaaatgcgcc
     5221 tcttgattgc ctgtgcgtcc ggcgcgggtt tggcggcggt gtataacgtg ccgctcgcct
     5281 ccacgctgtt tatcctcgaa gcgatgttgg gcgtgtggac gcagcaggcc gccgccgccg
     5341 cgttgctgac ttcggtcatt gccaccgccg tcgcgcgcat cggcttaggc gacgtgcagc
     5401 aatatcatcc cgccaacctt gccgtaaaca cctcattact ttggttttcc gccgtcatcg
     5461 gcccgatact gggcgcaacg gcggtatatt tccggcgcag tgccgaaaag ttccccttcc
     5521 tcaagcgcga caatccgaga attattcctt tggcgattgc tttgtttgca ctcatcggcg
     5581 cgattgccgt ttggtttccc gaaattttgg gcaacggcaa agcaggcaac caactgacct
     5641 ttggcggatt gaccgactgg caacacagcc ttgagctgac cgccgtcaaa tggctggttg
     5701 tcttgatggc gttggcggca ggcgcatacg gcggactgat tacgccgtct atgatgctcg
     5761 gcagcaccat ttcttttgca gccgccgccg cgtggaacag cgttttcccc gaaatgccgt
     5821 ctgaaagcgc ggcagtcgtc ggcgccgccg ttttcctcgg cgtttccctc aatatgccgc
     5881 tgaccgccgc cgtctttatt ttggaactca cctacgcccc cgccgctttg ttgatgcctt
     5941 tatgtacggg tatggcgggc gcggtatgga cggcaaggaa aatgggatgt aaataaccga
     6001 aaggcaaaat gccgtctgaa accggttttc agacggcatt cctttgggga ttgtcaacaa
     6061 tatctgcaaa aacctactgc cggaaagtgg aaaatagcgg ataataccgc cccgacaaca
     6121 ttaccgtttc ccatcccaac gtcagaaaaa ggaaatccct atgccttcca tcaaacgcgc
     6181 cctgatcagc ctgtccgaca agacgggcgc agtcgaattt gcccaaaccc tgaccaagct
     6241 cggtgtcgaa atcctctcta ccggcggcac ggcgaagctg cttgccgatg cgggcgttcc
     6301 cgtgattgaa gttgccgact acaccggttt ccccgaaatg ctcgacggcc gcgtgaaaac
     6361 cctgcatccg aaaatccacg gcggtatttt gggccggtgc gatttggacg aacacgtcgc
     6421 caagatggaa gaacacggca tcggcaacat cgacctcgtg tgtgtcaacc tctacccctt
     6481 cgccgccacc atcgccaaac caaactgcac gctggaagac gcgattgaaa acatcgacat
     6541 cggcggcccg accatggtgc gctccgccgc gaaaaactgg aaacacgtcg ccatcgttac
     6601 cgacacagcc gatttcccgg ccatcgccgc cgaactcgaa gccaacaatg gcgcattgag
     6661 cgacaaaacc cgcttcaacc tgtcgcgcaa ggcgttcagc cacaccgccc aatacgacgg
     6721 catgatttcc aactacctga cctcactttc agacgacgtt ttgagcggag agcctgaaat
     6781 cggcgaattt ccaagccagt tcaaccaaag ctggattaaa gtgcaagaca tgcgctatgg
     6841 cgaaaacccg catcagcgcg ccgcgttcta ccgcgatatt gaccccgccg caggcagcct
     6901 ttccgcctac aaacaactgc aaggcaaaga attgtcttac aacaacatcg ccgatgccga
     6961 tgccgcatgg gaagccgtca aatcctttga cgcgccagcc tgcgtgatcg tcaaacacgc
     7021 caatccgtgc ggcgtcgccg ttgcagccga tacattgacc gcctacaaac tcgcctacgt
     7081 caccgacacc accagcgcgt tcggcggcat catcgccttc aaccgcgaag tggacggcga
     7141 aaccgtcaaa cagattaccg acaaccagtt tatggaagtc ttgatggcgc cgaagtttac
     7201 cgccgaagcc ctcgaaatcg ccgccgccaa gaaaaacgtg cgcgtattgg aagtgccgct
     7261 tgaggcaggc gcaaaccgct tcgaactcaa acgcgtcggc ggcggactgt tggtacaaac
     7321 ccctgatatt caccgcctca accgcgccga tttgaaagtc gtctccaaac gccaaccaac
     7381 cgagcaagaa tggaacgacc tgatgtttgt ttggaacgtc gcaaaatacg tcaaatccaa
     7441 cgccatcgtc ttcggcaaag gcggtcaaac ctacggcatc ggcgcaggcc aaatgagccg
     7501 cgtggacagc acccgcatcg ccgcccgcaa agcgcaagat gccaatctcg acctcaacgg
     7561 cgcatgtgcc gcttccgatg ctttcttccc cttccgcgac ggcgtggacg tgattgccga
     7621 gcagggcatc aaagccatca tccatccggc aggttcgatg cgcgatcagg aagtcttcga
     7681 cgcagcggac gaacacggca tcgccatggt cgtaaccgac gtgcgccact tccgccatta
     7741 acgcagatga acaaggcaat gccgtctgaa gggctttcag acggcatttt gcgctatttt
     7801 gtgaaggtag ggatgacggt tcgggtattc ctgacagggt ggattttcaa ggtgttgtat
     7861 agggtgtagg aggattcgta aaaggtggga tgcagggtgt gcttcagccc gctgcatcaa
     7921 aaatttttgg agaatcggcg ggagtcggcg gttttggttt cggcggggac ggtggaaatg
     7981 ggtaacattg acggaatcga cggaagcggt gggcttcagc ccaccgcttg tatattggaa
     8041 tcaccgtatc atagcgccac ccgcgcccac ccaaggcaca caaccgttgc gtagctcagg
     8101 gagcggcagg gcaacccatc gacacaaccg gacagttgcc ggacaacaca accgaatgca
     8161 aggcaggttt atgatgagta cccaatacca ttacgcaggt atagtgaatt aaatctaaac
     8221 cagtacagcg ttgg
//
LOCUS       DAWKST010000087         8135 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.087, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000087 DAWKST010000000
VERSION     DAWKST010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8135
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.087"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(67..291)
                     /locus_tag="WJL87_001325"
     CDS             complement(67..291)
                     /locus_tag="WJL87_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEZ7799749.1"
                     /translation="MDAVQELEHRITELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(292..1680)
                     /locus_tag="WJL87_001326"
     CDS             complement(292..1680)
                     /locus_tag="WJL87_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ7799750.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGMMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(1766..2587)
                     /gene="prmC"
                     /locus_tag="WJL87_001327"
     CDS             complement(1766..2587)
                     /gene="prmC"
                     /locus_tag="WJL87_001327"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ7799751.1"
                     /translation="MTFDEWLGLSKLPKNEARMLLQYASEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYLLGWREFYGRRFAVNPNVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISPPSLETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLSQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(2594..3037)
                     /locus_tag="WJL87_001328"
                     /pseudo
     CDS             complement(2594..3037)
                     /locus_tag="WJL87_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3102..4586)
                     /gene="tldD"
                     /locus_tag="WJL87_001329"
     CDS             complement(3102..4586)
                     /gene="tldD"
                     /locus_tag="WJL87_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ7799752.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRESYASVPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     regulatory      4764..4867
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4934..6157
                     /gene="cytX"
                     /locus_tag="WJL87_001330"
     CDS             4934..6157
                     /gene="cytX"
                     /locus_tag="WJL87_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ7799753.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRQARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGVSANNISAKLSETPIAVAVAVVGTLLAVLLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     gene            6154..7254
                     /locus_tag="WJL87_001331"
     CDS             6154..7254
                     /locus_tag="WJL87_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ7799754.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVVRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPEDLQAQYDWLIDCRGYGAKTAWNASAEHGSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVVGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYSLARRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALVDGKDAPERDEESGLAYIRRQD"
     gene            7275..7892
                     /gene="thiE"
                     /locus_tag="WJL87_001332"
     CDS             7275..7892
                     /gene="thiE"
                     /locus_tag="WJL87_001332"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ7799755.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKTLHGDE
                     LKREIARCVAACQGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYVASGAIFPTTTKQMPTAPQGLDKLCEYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAENPEAVVKAFQALWDG"
BASE COUNT         1735 a         2504 c         2206 g         1690 t
ORIGIN      
        1 ttaggtttgc atcggcggaa tattcaaaca cagccttttt taaggaaatc cggatacggc
       61 ggcgcatcaa taatgcggcg gaatctcgtc gcgcagggaa tacggctctt gcgcgtcggg
      121 attcctgtcc tgcatttttt gatacagcag cctcaactga gcctgctgca aatccagcgt
      181 ctgccgcaat tccgccacca tcgcgttcag gccggcgatt acgtcctcct gaagcgcgga
      241 ttggatttcc agctctgtga tgcggtgttc caactcttga accgcgtcca tttacagcac
      301 catcgcggca atccagccgg caatcagcag cgggatattg tagtggatga aggtcgggat
      361 aacggaatcg cggatgtggt cgtgttgtcc gtcggcgttc agccccatcg tcgggcccaa
      421 cgtggaatcg gacgcaggcg aaccggcatc gcccaacgcc cccgccgtgc cgacgatggc
      481 gacggtggca agcggcgaaa aacccaaacc gacacacaaa ggcacataaa tcgcggcaat
      541 aatcggcaaa gtggaaaagg acgaaccgat gcccatcgtt accaaaagcc ccaccaccag
      601 catcgccaat gccgccatac ctttgctgtt gccgaatatc gccatactgc tttccaccag
      661 cggctgaata tgcccggtcg cattcatcac ggcggcaaaa ccctgcgcgg caatcataat
      721 gaagccaacc atcgccatca tcttgatgcc ttcgccgaat acgtcgtttg ccttgtcgcg
      781 gttgatgacg cccaacatca taaatacggc gaaaccgagc atcgcgccca acaccagcga
      841 gtcttcatac atcaactgga tggcaaagca tacggcaatg gcggcggcgg cggccaggct
      901 gcggtaggcg gacggctgcg gacggtttgc cgcatcggcg ttgcccgccg tatcggcatt
      961 gttgctttgg tacaggcgcg gtttgcggta atggacaaac gccagcagga gtccggccag
     1021 cattcccaac gcgggaatcg ccattgccgc cattacgttg atgtttttca catcaagctg
     1081 cggcgcggcg gaatggatgt tgcccaacag gatttcgttc aaaaaaatcg cgccgaagcc
     1141 gtaaggcagg aacatatagg tcgtaaccag cccgaaagta atgacgcacg caatcaggcg
     1201 gcggtcgatt ttcaggcggt tgaacaccaa aagcagaggc ggaacaatca tcgggataaa
     1261 ggcaatgtgg atggggatga tgttctgact catcatgccc atcacaagga tgatggaaag
     1321 cagcagccat ttgaccgcgc cctcgcccga acgcacgctg tcgggcatac cgccccggtt
     1381 cagcttgcgg acgaccgcgc cggcaagctg ctgcggcagg ccggaatggg taatcgccat
     1441 tgcaaacgcg ccgagcatcg cataagaaag cgcaatcttc gcaccgcctt ccaaaccttt
     1501 gttgaacacg gggataatcc ccgcctggct gacctgtccc gccgcatcgg caatgttttg
     1561 cagcggcata cccgccaccg cgccgccgac aaacgcgccg accgtcaggc tcaataccac
     1621 gtgcacgcgc gacagcgaca gcaccagcat aacgattacg gcaactacga ctgcattcat
     1681 tgtttatcgc tccaaaccta taaatgttta catatcgaaa cacatcataa cccaataacg
     1741 gaaaacccgc caattttgca aacaattatt tcaaatgctt catatacttc cccagcgtaa
     1801 ccctgtccaa acccgccaag tccggcaggg tttccactcc cgaaaaacca ttctccgcca
     1861 acacgccgcg caccgccgcg ccctgatcga aaccgtgttc cagcaataaa aaaccgcctt
     1921 ccgccaaacg gtcgggcgcg ccttgcgcca aggtgcggat gcagcttagg ccgtctgaaa
     1981 agtcggtcag cgcgatttgc ggctcaaacc gcaaatcgcc ttgcgacaaa tgtttatcac
     2041 cgttttcgat atagggcggg ttggacacga tgatgtccca tttcccttca gacggcatat
     2101 cggtgtcgaa ccacgaaccg tgtgcaaatt cgacccgcgc gcccaaatcc gccgcatttt
     2161 tccgcgccgt ttcaagggag ggcgggctga tgtcggatgc gcgcacaaac gcatcggggc
     2221 gttcgagcgc gacggttacg gcaaccgcgc cgctgcccgt ccccaaatcc cacacgcgcc
     2281 cgttttccgg caggcgcgcc aatacggctt cgaccaaatg ttcggtttcg gggcgcggaa
     2341 tcagcacatt cgggttgacg gcgaaacgcc gtccgtaaaa ctcgcgccag cccaacagat
     2401 aggcaaccgg ctcgccgttc agacggcgtt gcgccagcct gtccgcccgc tgtcggactt
     2461 cgtccggcat ttcttcaccg ccccgcgtca acaactgcac gcgcgtatat tccgaagcat
     2521 attgcagcag cattcttgct tcatttttgg gcagttttga caagcccaac cattcgtcaa
     2581 acgtcatttt tatcccgtct gccgctgatg cggcttttct ttccttattc tttccggcaa
     2641 acgtaccgat ggtggcaacc gcaaatgcgg cataccacaa ataaaatcct gcaccgtagc
     2701 gcacaatatc cgatgtattc cctgcttcat cgacgtatac ggctttcaca ctgaaagcca
     2761 ccaacgccaa gccccaaagt gccgcatgga caggcacgac cttcttccgc aacgccagca
     2821 aaacaatggc cgccaaccaa acataattcg catagaccgc acaatacctg atatccaaag
     2881 aagcaaatat cgaccccaaa atcaaaacgg tcaaaccctc catgcttcca tgattgccca
     2941 aataaaatgc aacattggat aaagacgcta tccacagggc aaccgacacc agcaacatca
     3001 ctatgggaaa acttgatttc cgattctgtt cctgcatggt tttatcctaa tgtaaaaggc
     3061 cgcctgaaaa cctttcagac ggcatcgtgc cggattccgc gtcagattgc gctgccgccg
     3121 acggtcagcc cggcatcgat acgcagggtc ggctgcccca cgccgacggg gacgctctgc
     3181 ccttctttgc cgcacacgcc gacaccgctg tccaacgcgg tatcgttgcc tatcatcgaa
     3241 acgtgtttca gcacttcggg cccgttgccg ataatggtcg cgcctttgac ggggtattgc
     3301 agcctgccgc cttccaccca ccatgcttcg gacgcgctga acacaaattt gccgctggta
     3361 atgtccacct gtccgccgcc aaagttgacg gcgtaaatgc ctttgtcgat ggacgcgatg
     3421 atttcttccg gctcatagct gccattttcc ataaaggtat tggtcatgcg cggcatcggg
     3481 acggaggcgt agctttcgcg gcggccgttg ccggtggatt gcgtacccgt caggcgggca
     3541 ttggtttcgt cctgcatata gccgactaaa atgccgtctt caatcaatac ggtgcggcgg
     3601 gtttcgttgc cttcgtcgtc gatgttgagc gaaccgcgcc ggccggcaat atcgccctga
     3661 tcgacgacgg taacgccttt ggcggcgacg cgctcgccta ttctgccgga aaagacgctg
     3721 gtccccttgc ggttgaaatc gccttccaaa ccgtgtccga ccgcttcgtg cagcaacacg
     3781 cccggccagc cgttgcccaa aacgacggtc atttcgccgg cgggcgcggg gcgggattcg
     3841 aggttggtga gtgcctgttt gacggcggca tcgacaaact gccgcaccaa gttttcatcg
     3901 aaataagcca agtcgtagcg tccgccgccg cccgcgctgc cctgttcgcg gcgttcgccc
     3961 tgtttggcga tgacggtaac gttcaggcgc accatcgggc ggatgtcggc ggcgtgtttg
     4021 ccgtccagac gggcgaggta aaccatatcg tattcgcagg tcaaaccggc catcacttgc
     4081 acgatgcgcg gatcggcggc tttggcgatt gcttccactt tgttcaacag cgcgactttg
     4141 gcggcggaat cgaggccggc aatggggtcg gacgcggaac aaaccggctt gccgcgcgtt
     4201 tcagacggca ttttggcgga caccttgccg cctgccgccc caatcgcgcg gacggcgcgg
     4261 gcggaacggt ttatcgaatc gatgcacagg ctgtcggcgt aggcaaaggc ggttttgtcg
     4321 cccgaaacgg cgcgcacgcc cacgccctga ttgatttgga agctgcccga tttgacgatg
     4381 ccctcttcca aatgccagct ttcataagcg gtgcgctggc agtagatgtc ggcgtaatcg
     4441 acgtggtgcg cgccgatgat gcacaggctt ttggcgagca gttcggggga aaggcggttg
     4501 gcttcgagca gccgcgcctg tacggcggaa taggtcggat gcatagtgtc ggcgcataaa
     4561 aaatcagggg cttgattata cggcatttgt tatatagtgg attaacaaaa aacagtacgg
     4621 tgttgcctcg ccttgccgta ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt
     4681 tttgttaatc cactatagaa atgcgccgtg ccgcctgaaa tgtaagattt ttgccaacgc
     4741 cccctgcttt tgtgtacact taaagctcct tgtcggagtg ccgccgccgg gcggctgaga
     4801 ttgcgaaagc agaatccgta gaacctgtcg gggtaatgcc tgcgtaggaa acaaaccgtc
     4861 aaatgcctta tcaggcttcc gttccctttt ccgcacttcc ccgcccacct tccttctttt
     4921 taaaggacgt catatgtcgg gcaatgcctc ctctccttca tcttccgccg ccatcgggct
     4981 gatttggttc ggcgcggcgg tctcgattgc cgaaatcagc acgggtacgc tgcttgcgcc
     5041 tttgggctgg caacgcggtc tggcggctct acttttgggt catgccgtcg gcggcgcgct
     5101 gttttttgcg gcggcgtata tcggcgcact gaccggacgc agctcgatgg aaagcgtgcg
     5161 cctgtcgttc ggcaaacgcg gttcagtgct gttttccgtg gcgaatatgc tgcaactggc
     5221 cggctggacg gcggtgatga tttacgccgg cgcaacggtc agctccgctt tgggcaaagt
     5281 gttgtgggac ggcgaatctt ttgtctggtg ggcattggca aacggcgcgc tgattgtgct
     5341 gtggctggtt ttcggcgcac gcaaaacagg cgggctgaaa accgtttcga tgctgctgat
     5401 gctgttggcg gttctgtggc tgagtgccga agtcttttcc acggcaggca gcaccgccgc
     5461 acaggtttca gacggcatga gtttcggaac ggcagtcgaa ttgtccgccg tcatgccgct
     5521 ttcctggctg ccgctggccg ccgactacac acgccaagca cgccgcccgt ttgcggcaac
     5581 cctgacggca acgctcgcct atacgctgac gggctgctgg atgtatgcct tgggtttggc
     5641 agcggcgttg ttcaccggag aaaccgacgt ggcaaaaatc ctgctgggcg caggtttggg
     5701 tgcggcaggc attttggcgg tcgtcctgtc gaccgttacc accacttttc tcgatgccta
     5761 ctccgccggc gtaagtgcca acaatatttc cgccaaactt tcggaaacac ccatcgccgt
     5821 tgccgtcgcc gttgtcggca cactgcttgc cgtcctcctg cccgttaccg aatatgaaaa
     5881 cttcctgctg cttatcggct cggtatttgc gccgatggcg gcggttttga ttgccgactt
     5941 tttcgtcttg aaacggcgtg aggagattga aggctttgac tttgccggac tggttctgtg
     6001 gctggcgggc ttcatcctct accgcttcct gctctcgtcc ggctgggaaa gcagcatcgg
     6061 tctgaccgcc cccgtaatgt ctgccgttgc cattgccacc gtatcggtac gccttttctt
     6121 taaaaaaacc caatctttac aaaggaaccc gtcatgaccc gtatcgccat cctcggcggc
     6181 ggcctctcgg gaaggctgac cgcactgcag cttgcagaac aaggttatca gattgcactt
     6241 ttcgataaag gctgccgccg gggcgaacac gccgccgcct atgttgccgc cgccatgctc
     6301 gcgcctgcgg cggaagcggt cgaagccacg cccgaagtgg tcaggctggg caggcagagc
     6361 atcccgcttt ggcgcggcat ccgatgccgt ctgaacacgc acacgatgat gcaggaaaac
     6421 ggcagcctga ttgtgtggca cggacaggac aagccattat ccagcgagtt cgtccgccat
     6481 ctcaaacgcg gcggcgtagc ggatgacgaa atcgtccgtt ggcgcgccga cgacatcgcc
     6541 gaacgcgaac cgcaactcgg cggacgtttt tcagacggca tctacctgcc gaccgaaggc
     6601 cagctcgacg ggcggcaaat attgtctgca cttgccgacg ctttggacga actgaacgtc
     6661 ccttgccatt gggaacacga atgtgccccc gaagacctgc aagcccaata cgactggctg
     6721 atcgactgcc gcggctacgg cgcaaaaacc gcgtggaatg cttctgccga acacggcagc
     6781 accctgcgcg gcatacgcgg cgaagtggcg cgggtttaca cacccgaaat cacgctcaac
     6841 cgccccgtgc gtctgctcca tccgcgttat ccgctctaca tcgccccgaa agaaaaccac
     6901 gtcttcgtcg tcggcgcgac ccaaatcgaa agcgaaagcc aagcccctgc cagcgtgcgt
     6961 tccgggctgg aactcttatc cgcactctat gccgtccatc ctgccttcgg cgaagccgac
     7021 atcctcgaaa tcgctaccgg cctgcgcccc acgctcaacc accacaaccc cgaaatccgt
     7081 tacagcctcg cccgacgcct gattgaaatc aacggccttt tccgccacgg cttcatgatt
     7141 tcccccgccg taaccgccgc cgccgtcaga ttggcagtgg cactggttga cggaaaagac
     7201 gcgcccgaac gtgatgaaga aagcggtttg gcgtatatcc gaagacaaga ttaaagccgt
     7261 ctgaaaggaa ccccatgacc ttcccgccct tgaaatcccc gctcaaattc tacgccgtcg
     7321 tccccaccgc cgattgggtg gggcgcatgg tcaaagcagg tgccgacacg gtgcaactgc
     7381 gctgcaagac cctgcacggc gatgaattga aacgcgaaat cgcccgctgc gtcgccgcct
     7441 gtcaaggcag ccgtacgcag cttttcatca acgaccactg gcgcgaggca atcgaagcag
     7501 gcgcgtacgg cgtgcatctc ggacaagaag acatggacac cgccgacctt gccgccatcg
     7561 ccgccgccgg tttgcgcttg ggtttgagta cgcactccgt tgccgaactc gaccgcgccc
     7621 tgtccgtaca ccctagctac gtcgccagcg gcgcgatttt cccgaccacg accaagcaaa
     7681 tgcccaccgc cccgcaaggc ttggacaaac tgtgcgagta tgtgaaacag gcaggcggca
     7741 cgcccgtcgt cgccatcggc ggtatcgact tgaacaacgc ccgagccgta ctcgccaccg
     7801 gcgtttcctc actcgccgcc gtccgcgccg tgaccgaagc ggaaaatccc gaagcggtgg
     7861 ttaaagcgtt tcaggctttg tgggatggat aaaaccgaaa gaagaaaatt caatttgccg
     7921 tgtcggcaaa acttagcccg ttatcgcaaa catacttaac tttaaatgtg gcatatcatc
     7981 aaattccgtc atttccgcgc aagcgggaat ccgccttaaa acttgagaaa ccatcatttg
     8041 aaaaacagtt tccgaatttc aaaaatggat tcccgcctgc gcgggaatga cggcaaccgg
     8101 ccggttgcgt atcaaaaaat aaagtaattc ggcta
//
LOCUS       DAWKST010000088         8073 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.088, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000088 DAWKST010000000
VERSION     DAWKST010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8073
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.088"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            96..404
                     /locus_tag="WJL87_001333"
     CDS             96..404
                     /locus_tag="WJL87_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799756.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQIRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            486..1517
                     /gene="ruvB"
                     /locus_tag="WJL87_001334"
     CDS             486..1517
                     /gene="ruvB"
                     /locus_tag="WJL87_001334"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ7799757.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLIGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(1540..2268)
                     /gene="rpe"
                     /locus_tag="WJL87_001335"
     CDS             complement(1540..2268)
                     /gene="rpe"
                     /locus_tag="WJL87_001335"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ7799758.1"
                     /translation="MTAYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRRV
                     RAMLDRYEAQSGRHIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            2414..2848
                     /locus_tag="WJL87_001336"
     CDS             2414..2848
                     /locus_tag="WJL87_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ7799759.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQSQHNELRKIRTAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(2861..3724)
                     /locus_tag="WJL87_001337"
     CDS             complement(2861..3724)
                     /locus_tag="WJL87_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ7799760.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLVRLAGVGRQTELLVRNTEQFIVGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(3732..4349)
                     /locus_tag="WJL87_001338"
     CDS             complement(3732..4349)
                     /locus_tag="WJL87_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEZ7799761.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFHTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDGRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(4512..5126)
                     /locus_tag="WJL87_001339"
     CDS             complement(4512..5126)
                     /locus_tag="WJL87_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEZ7799762.1"
                     /translation="MKTSNFSKRTSAMKTFALILADGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPGDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQIDLQEG"
     gene            5332..7104
                     /locus_tag="WJL87_001340"
     CDS             5332..7104
                     /locus_tag="WJL87_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HEZ7799763.1"
                     /translation="MILPARFSDGISLSLRLKLLTGVWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRSQIDNQVDEFEKSLNRISQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGESDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFSDGISLTGDDRTLLQTLIRQLGVSLASAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENISFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIHAV
                     LEIRSQAQQGTTVSLTVASEESLK"
     gene            7101..7757
                     /locus_tag="WJL87_001341"
     CDS             7101..7757
                     /locus_tag="WJL87_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ7799764.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECIRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVLE
                     "
BASE COUNT         1982 a         2369 c         1889 g         1833 t
ORIGIN      
        1 agttttgagg acgataccca atccaagctt tgcaacagcc gccgccatat ccgctataat
       61 tcacgcttca gccatcctgc ccccgacata aaatcatgac cctgaaaacc gatttattgc
      121 ctaaaatcaa caacgaagat tatcaacgcc tcatcctcaa acacagtgcg gaatttagcg
      181 gtggcgaaat ccgcctgttg aacgaaatcc tcgaaaaatt caatttcgac gttgttcagg
      241 cgcaggcatt ggcgcaagcc gtcatgcagc aaatccgctt cgaccccaac gcctaccaca
      301 tcgacagcga cgacgaagac accaccggca tctgccccca ctgcatcaac ccgcctatgc
      361 cgcccctgcg cgactatctc gtttggcgcg aaacccgcgg ataaaacgct tttgaccgtt
      421 atcttttcaa tgccgtctga aacgccgccg accgttcgga cggcataccc gacaaaggga
      481 acactatgct gcaaaccgac aacctgaccg ccgcgcaacc gcaacgcatc gttgccgccc
      541 aaaccgcctc cgcacaggaa gaactgctcg aacgcgccct ccgccccaaa acgctggacg
      601 actacatcgg gcaagacaaa gccaaagaac agcttgccat tttcatccaa gccgccaaaa
      661 aacgcggcga agcactcgac cacgttttgc tcttcggccc gcccggactg ggcaaaacca
      721 cactggcgca catcatcgcc aaagaattgg gcgtaaattt gcgccaaacc agcggccccg
      781 tcctcgaacg cgcaggcgac ctcgccgccc ttttgaccaa ccttgatccg cacgatgtat
      841 tgttcatcga cgaaatccac cgcctcagcc ctgttgtcga agaaatcctc tatcccgcgc
      901 tcgaagacta ccggctcgac attatgatag gcgaaggacc cgccgcccgt tccgtcaaaa
      961 tcgacctgcc gcccttcacg ctcatcggcg cgaccacccg cgccggtatg ctgaccaatc
     1021 cgttgcgcga ccgcttcggc atcgtctccc gccttgagtt ttacgaaaac cgagacctta
     1081 ccaccatcgt cagccgttcg gcacaactgt tgcagctcga tatgtccgaa gaaggcgcgg
     1141 aagaaatcgc caaacgcagc cgcggtacgc cgcgcatcgc caaccgcctg ttgcgacgcg
     1201 tgcgcgattt cgccgacgtg aaaaacaacg gcacaatcga cggcggcatc gccgatgccg
     1261 ctttaagtat gctggacgtg gacgcgcagg ggctggacgt gatggacagg aaatttctcg
     1321 aagccgtttt gcacaaattc ggcggcggcc cggtcggttt ggacaatgtt gccgccgcca
     1381 tcggcgaatc tacagacacc atcgaagacg ttatcgaacc ctaccttatc caacaaggct
     1441 tcctgcaacg caccccgcgc ggcaggatgg cgaccgaacg cgcctacctg catttcgggc
     1501 tgcccgtcga aaaataacgc aatgccgtct gaaacagagc taattttcag acggcatttc
     1561 tatttcaatc attggcgcaa ggttcagcct gccgcttttt tccagttccg ccctcatcgc
     1621 atcaatcacc gccttatagt ctggtttgcc gaaaatcgca gaaccggcaa caaaggtatc
     1681 cgcaccagct cgggcaacgg cggcaatatt gtcggttttg atgccgccgt ccacttcgat
     1741 ggcgatgtgc cgcccgcttt gtgcttcgta ccgatccagc atcgcccgca cccggcggat
     1801 tttttcaagg gtgtgcggga tgaagctttg tccgccgaat ccggggttga ccgacatcag
     1861 caaaaccata tccagcctgt ccaatacgtt ttccaacaga tatacgggcg ttgccggatt
     1921 caacaccagc cccgcctgac agcccatatc acgaatcagg ctcaagctgc ggtcgatatg
     1981 gcggctcgct tcgggatgaa aagtgataat cgatgctcct gctttggcaa acgactgaat
     2041 caggtcgtca acgggttcga ccatcagatg cacatcgatc ggcacgcttg cataaggctt
     2101 caacgccgcg caaaccatag ggccgaaggt caggttcggc acataatggt tgtccatcac
     2161 gtcaaaatgg atcagatctg cacctgccgc aatgacgctt tccacctctt ctccgaggcg
     2221 ggcaaagtct gccgacaaaa tactgggtgc gatacggtag gcggtcatat tttttccttc
     2281 aatgccttta cgggcggact caacgcggga aagccccgaa acagcattat taacaaaagt
     2341 taactgccat aataccatct ttcacacggc aatcaagtat attgtccgaa atatctggat
     2401 taaaggaaaa gccgtgccac tgcctgctcc ctgccgtttt gccaaacctg ccgcctcttt
     2461 tttaagtatg gctttgcttt cctgtcagct ttcccacgcc gccacggctt atatcccccc
     2521 gaacgatttt caaccgaact gcgacatacg ccgactcggg ctgacccaaa gtcagcacaa
     2581 tgagctgcgt aaaatccgca ccgccttcaa aatggcgggc gacagggcgc gtttgaaggt
     2641 tatgcattcc gaacacagcc gccgccggtc tgtcgtcgaa atcatttcct cggatgtttt
     2701 taatcggaac gaggcgcgcg attatgtcga aagccgctat ttgtccggta tggattttgc
     2761 ggtggacgaa ttggaaatcc aacaccggtt cttccatatc ctcacaccgc aacagcagca
     2821 aatgtggctt tcttcctgcc tcaaataatc cccgaaacgc tcacaacgcc cgttgtttcg
     2881 gcagcctgcc cgcccagtcg caggcaaact gccacgcgga acgtcccgaa cggctgcccc
     2941 gcgtctgcgc ccactgcaat gccgccatct gtgcggtttc atcatacggc acgccgaaat
     3001 cttccagcca attttgcacc gccgccagat aatcgttttg atcgaacgga taaaaactga
     3061 gccacaatcc gaatcggtcg gacagggata ttttttcttc caccgcttct ttctgatgga
     3121 tttccccccg catccccgtc gtaccggcat tctcgtcaaa atattcgggc atcaggtgcc
     3181 gtctgttgga agtcgcgtaa accaaaacgt tggcgcaacg ttgagacaga ccgccgtcta
     3241 acgcggtttt caatgcctta taggtttcat cgccgctttc aaacgacaaa tcgtcgcaaa
     3301 atacgataaa tttttccgga cgttccttca aaagcgtcaa caggtaaggc aggccgatta
     3361 aatcgctttt atcgacttcg atcaggcgca atcccttatc cgcatattcg tgtagcaggg
     3421 ctttgaccag cgaggatttg cctgttccgc gcgcgccgct catcaataca ttgttcgcgg
     3481 gtctgccgac aatgaactgt tcggtattac gcaccagcaa ttcggtttgc ctgccgactc
     3541 ccgccagcct taccaaggga aaggtgtgcg gatcgggcaa gtgttccaaa aaaccttttt
     3601 tgcccgcact ctgccagcgg aaggcaagcg cgttccaatc cgtatgcccg ggttcgggcg
     3661 gaagcacggc atccaaacgc cgcaaaacgg cataggcttt atcgagaaat tcgttcaatt
     3721 ccatctctgc ctcactttgc atatctttgc gccatcagcc gttcgacggt atcgacgatt
     3781 gcctgcgtat tcggatcgat ttcgatgttg atcctgtcgc cgacctttct gctgccgaac
     3841 agcgtccgtt ccaaagtttc gggaatcaga tggacattga aacggccgtc ttcgactttg
     3901 cctatggtca ggctgcaacc gtccaagccg acgaaccctt tggtcaggat atagggtttg
     3961 agttcatgcg ggagcgaaaa ccaaaccgtg cggttgaacc cgtcccgttc gatttcgaca
     4021 ataggcacgg ttgccataat gtgtccgctc atgacgtgtc cgccgatttc gtctccgaaa
     4081 cgcgccgccc gttcgatgtt gacgcaatcg ccttccttca gcagccccaa attggttttt
     4141 gccaaagttt ccgccattaa atcgaaactg acgcggtttc cttcgatttc ggtaatcgtc
     4201 aggcagcagc cgttattggc gaccgatgcg ccgcgttgca gattgtccgc cgcctcttgc
     4261 ggaagctcga cgacataagt gtgaaatgcc tccgacgggc ggtggattgc cgtcagtttt
     4321 cccaatcctt gaacaatgcc tgtaaacata atcctgtttc cctgtgtcgg taaaaatggt
     4381 gcaaattgta gcatctcccc gcgaaaaatg ccgtctgaaa tgccttcaga cagcattatg
     4441 cctccgattc gggcaaaaac cgcccggtat ggcttgacct ttcctttcca cgccggtcgg
     4501 cggtcttgcc cttatccctc ctgcaaatcg atttgcgtgt tcaagtcggc aaaatgcccg
     4561 tcaaactcga atctgaccgg ccgcgccctt tgctgctgca accagcttct taatgttttc
     4621 atacccgaaa acagataggg aatcgcgctt tgcagaatct gcgggcggat atacataatg
     4681 ttgtagtgca tcgttatcgg cgtttccaca taatacgcat tgcacaacgg tgtgcgtttc
     4741 gacacggttt caaacctcgc caccaaatcg cccggcagat acggcatatc gcacggcaca
     4801 accaaaagcc agtcggcagt cgccaactgc aaatcgttgg ctgcggtaca caatgccgaa
     4861 agcgggccga aatgctgcca ctgccgtgcg tccggaaaaa tatgcggact tcttcgagca
     4921 tattcttcca aattccggtt ggtgctgatg gcgatatggc tgacctgcgg cctgaccctg
     4981 tcgatgacat gatctatcag tgccttaccc cccaaaagag caagcccctt gtcctcgcct
     5041 cccatacggc tcgcctgacc gtcggccagt atcagggcaa aagttttcat tgcggatgtt
     5101 ctcttggaaa agttcgaggt tttcatgatt gcagtctgcc gttcccaatg actcaaaatg
     5161 ccgtctgaag cagacggcaa ataaattcat attatctgaa ttttatcata acatgattta
     5221 acactgaagc ggtacggatt atgttttcct taaccaattc tttctgagca gttgtatcta
     5281 atcccaaaga atgatattgt ttgcattatt tagaacaatt tttcgccgag catgatactg
     5341 ccagcccgtt tttcagacgg catcagcctt tccctacgcc tgaaactcct gaccggagtg
     5401 tgggtcgggt tggcggcatt gtctgtcgtt ttgacactgc tgctctcttt gcgtctggaa
     5461 aacgcggcct ccgtcatcga agaggcgggc aacttgagaa tgcaggcata ccgtctggca
     5521 tacatggcgg gtgaaggatc tccccgctcc caaatagaca atcaggttga cgaatttgaa
     5581 aaaagcctga atcgtatttc acaaagtgat gccatccatc cgctgattcc ttcggacacc
     5641 cctcttgctt atgatttgat acaatccatg ctgattatag attggcaggc acacatcctc
     5701 cccccgctcc agtcctaccg gcgaccgact caggtcgatc tctaccgctt tgccggaaac
     5761 atcgaactgt ttttgcaggc attggaaaat gccaacgaaa aaaacacatg gtggctcagg
     5821 cgttttcaat gggcaattat gttgatgacg ctggtgtcgt ctgtactgat gctgttttgg
     5881 caccagattt gggttatccg gccgctgcag gcgttaaggg aaggtgcgga acgcatcgga
     5941 cggaggtgtt tcgatattcc ggttcccgaa ggcggtacgc cggaattcaa acaggtcggg
     6001 cgttgtttca atcaaatggg cggcaggttg aaaattttat atgatgattt ggaaggacaa
     6061 gtcgccgagc agacacgcag tctcgaaaaa caaaatcaaa acctgaccct gctgtaccaa
     6121 actacacggg acctgcacca atcctacata ccgcaacagg ctgcagaaca ttttctaaac
     6181 cgtatcctgc ccgccgtagg agcagattcc ggcagagttt gtttggacgg cgaatccgat
     6241 gtttatgttt ccattcatca tgcggattgc ggcacagcag cttcggattt ggggaagtac
     6301 catgaggaaa tcttccccat tgagtaccag aacgaaacat tgggcaggct gttgctcagc
     6361 ttttcagacg gcatttccct caccggagac gaccgcacgc tgcttcaaac cctgattcgg
     6421 caattgggcg tatcgctcgc tagcgcgaag caggaagaag aaaaacgcct gcttgcagta
     6481 ttgcaggaac gcaacctgat tgcgcaagga ttacatgaca gcatcgcaca agcattaacg
     6541 ttcctaaacc tacaggtaca gatgctggaa accgcctttg ccgaaaacaa acgggaggaa
     6601 gccgcagaaa acatcagctt catcaaaaca ggcgtgcagg aatgttatga agatgtccgc
     6661 gaactgctgc tcaacttccg taccaaaatc agcaataaag aatttcccga agccgttgcc
     6721 gacctattcg cccgctttac gcaacaaacc gggataacgg tcgaaaccgc ctgggaaaac
     6781 ggttcgttcc tgccgcctca ggaagcgcag ctccaaatga tttttatcct gcaggaaagc
     6841 ctgtccaaca tccgcaaaca cgcccgcgcc acccatgtaa aattcaccct ttccgaacac
     6901 ggcggacgct ttaccatgac catccaagac aacggacaag gtttcgacac ggagaaaata
     6961 ggagaaccca cgggcagcca tgtcggactg cacatcatgc aggagcgtgc caaacgcatc
     7021 catgccgttt tagaaatccg ttcccaagct caacagggaa ccaccgtctc attgacggtt
     7081 gcatctgaag aaagcttgaa atgactatta aaattattct gatagacgac cataccctct
     7141 tccgcagcgg cattaaagcc cttttgtcgc gccaacacgg ttttgaagtc atcggcgaag
     7201 ccgcagacgg cctctcgggc atcaaaatga ttagtcggct gcaacccgat gtcgtcctgc
     7261 tcgatttgga tatgcccggc atgaacggac gcgaagcact ctcccaaatc atcagcatca
     7321 atccgcagca ggcagtgatt atgctgaccg tttccgaaga cagcgacgac ttgaccgaat
     7381 gtattcgcat cggcgcgcgc ggttacctgc tgaaaaacat caacgctgac tttctgctcg
     7441 aaagcatacg caaagccgcc gaaggcgata atgtattctc gcccgagatg accgccaaac
     7501 tcgttaaaag cctgatttcc ccccaacctg cccaagggac acaggcactc tcctcactta
     7561 cccctcgcga actggaaatc ttgggctatc tcgccgcagg acacagcaac aaaatcatcg
     7621 cccgccacct cgatcttgcc gaatccaccg tcaaagtcca cgttcaaaac ctgctccgca
     7681 aactcaacct cagcagccgg gtgcaggccg ctgtttacgc catccggcac aacgtccccc
     7741 aacccgtgct ggaataaggc gggttcagac ggcatattag gggttttaat ccccgtacgg
     7801 tcattcggat aacagaccaa gcatgtaagt ttatgccccc ataagtacgc ttggcatagc
     7861 agtaatattg ttcggtttag tgttttccgt ttgcccctat ctgatactgc aatatcagct
     7921 atgccgtctg aaaacgtatc atcatgatat tttcagacgg cataataaaa agcggaaata
     7981 ctaatgcggg gtaaaatgtt ccattccgaa tcccataaat atacaatggc ttatatcgtt
     8041 taagcatgtg taacccaccc tcatatcaat caa
//
LOCUS       DAWKST010000089         8046 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.089, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000089 DAWKST010000000
VERSION     DAWKST010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8046
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.089"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(79..1425)
                     /gene="mnmE"
                     /locus_tag="WJL87_001342"
     CDS             complement(79..1425)
                     /gene="mnmE"
                     /locus_tag="WJL87_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ7799765.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAEALCGKTPKP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGVNDKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGEGEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1556..1711
                     /locus_tag="WJL87_001343"
     CDS             1556..1711
                     /locus_tag="WJL87_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799766.1"
                     /translation="MEKRNTYAFLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1708..2139
                     /locus_tag="WJL87_001344"
     CDS             1708..2139
                     /locus_tag="WJL87_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEZ7799767.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2273..6637
                     /gene="app"
                     /locus_tag="WJL87_001345"
     CDS             2273..6637
                     /gene="app"
                     /locus_tag="WJL87_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ7799768.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFTYKIVKRNNYKAGTNGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGRKYIDQNNYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPRQNGKYSFHD
                     NNNGTGKIDAKHEHNSLPNRLKTRTVQLFNVSLSETAQEPVYHAAGGVNSYRPRLNNG
                     ENISFIDEGKGELILTSNINQGAGGLYFQGDFTVSPENNETWQGAGVHISEDSTVTWK
                     VNGVANDRLSKIGKGTLHVQAKGENQGSISVGDGKVILDQQADENNKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKEST
                     VTITGNKDIATTGNNNSLDSKKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLS
                     GGTNLNGNITQTNGKLFFSGRPTPHAYNHLNDHWSQKEGIPRGEIVWDNDWINRTFKA
                     ENFQIKGGQAVVSRNVAKVKGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEK
                     TITDDKVIASLTNTDISGNVSLADHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNG
                     DLSLVGNAQATFNQATLNGNTSASGNASFNLSDHAVQNGSLTLSGNAKANVSHSALNG
                     NVSLADKAVFHFESSRFTGQISGSKDTALHLKDSEWTLPSGTELGNLNLDNATITLNS
                     AYRHDAAGAQTGSATDAPRRRSRRSLLSVTPPASVESRFNTLTVNGKLNGQGTFRFMS
                     ELFGYRSDKLKLAESSEGTYTLAVNNTGNEPASLEQLTVVEGKDNKPLSENLNFTLQN
                     EHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAEKDNAQSLDALIAAG
                     RDAVEKTESVAEPARQAGGENVGIMQAEEEKKRVQADKDTALAKQREAETRPATTAFP
                     RARRARRDLPQLQPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDELDRVFAEDR
                     RNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENTFDD
                     GIGNSARLAHGAVFGQYGIDRFYIGISAGAGFSSGSLSDGIGSKIRRRVLHYGIQARY
                     RAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQHI
                     SITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAAAK
                     GPQLEAQHSAGIKLGYRW"
     gene            complement(6746..7468)
                     /locus_tag="WJL87_001346"
     CDS             complement(6746..7468)
                     /locus_tag="WJL87_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ7799769.1"
                     /translation="MFPDKYKLSLKENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASVSLEDIQTILNLKRAYQYVISHISNGEPVNISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGIDYFGTHEDIDFPDPPNMG"
BASE COUNT         2131 a         2227 c         1948 g         1740 t
ORIGIN      
        1 aaccaaacgg atcggattcc cgtttttatg ggataacgga atgttcagcc ggacaccgcc
       61 acgatgcttt cgatccgttt attttccgat acaaaacctc gaaaaaatca cgccgagcag
      121 gtcgtccgcc gtaaactcgc ccgtgatttc gccgcatgcg acctgcgcca agcgcaagtg
      181 ttcggcaaac agctcgattt gatggttgcc gcacaatgcc gccagcgaca attcttcctg
      241 cgctgctttg agtgcgttga cgtgccgcgt ccgcgccaaa aacagccctt cgccctcgcc
      301 ctgccatccg gcctcgcgca acaacgtccg tttcagcgcg tccaagccgt cgccggtttt
      361 cgccgacagc gcgatgacgg tttccgcgcc cgtaccgaac ccgcctgccg cgtgtgcgtg
      421 caaatcggat ttgctgtgga tttcgatgcg tttcaactcc ggcggcaacg cgtccaaaat
      481 cgcccgtgtc ttgtcgttca cgccttcgcg cggatcgacc aacaccagcg cgacatcggc
      541 ttcggatacg gctttgcggc tgcgttcgat gccgatgcgt tcgaccacgt cgtccgtctc
      601 gcgcaaacct gccgtatcga caatatgcac cggaacgccg tcaatcagga tacgttccct
      661 gaccgcgtcg cgcgtcgttc cggcaatatc ggtaacaatc gccacttcgt cgcccgccag
      721 cgcgttcaac aggctggatt tgccgacatt cggcgcgccg accaatacga cattcagacc
      781 ttcgcgcaaa atcgcgccct gctgcgcgtt ggcaagcaca tcatccacgg cgcggcgcaa
      841 gccgtccagt ttgccgcgtg cgtctgccgc ttcgagaaaa tcaatgtctt cctcgggaaa
      901 atctaacgtc gcttcgacca gcatccgcaa ggtaatcaag tcttcgacca gaccgtgtat
      961 ccgccgcgaa aaatcgcctt tgagcgagcg caacgccaga cgcgccgccg aacggctgga
     1021 tgcgtcaatc aaatccgcca cgccttccgc ctgtgccaag tccagtttgt cgtttaaaaa
     1081 cgcacgcttc gtaaactcgc ccggttcggc aaggcgcgcg cccaattcca aacagcggtt
     1141 cagcagcata tccatcacta ccggcccgcc gtgtccctga agctcgataa catcttcgcc
     1201 cgtaaaactt gccggtgcgg caaaaaaaag caaaagcccg ctgtcgattg cctgaccgtc
     1261 cgcgtccgta aaatcagcat aggttgccac gcgcggcttg ggcgttttcc cacacaaagc
     1321 ctctgccatc ggcagcaggt ttttccccga tatgcgtatc acgcccacgc cgccgcgccc
     1381 gggtgcggta gcgactgccg caatcgttgg aacgttatcc gacataaaac ccccgaaaat
     1441 tcaaaacagc cgcgattata gcaaatgccg tctgaagtcc gacggtttgg ctttcagacg
     1501 gcataaaacc gcaaaaatgc ttgataaatc cgtccgcctg acctaatata accatatgga
     1561 aaaacgaaac acatacgcct tcctgctcgg tataggctcg ctgctgggtc tgttccatcc
     1621 cgcaaaaacc gccatccgcc ccaatcccgc cgacgatctc aaaaacatcg gcggcgattt
     1681 tcaacgcgcc atagagaaag cgcgcaaatg accgaaaacg cacaggacaa ggcgcggcag
     1741 gctgtcgaaa ccgtcgtcaa atccccggag cttgtcgagc aaatcctgtc cgacgagtac
     1801 gtgcaaataa tgatagcccg gcgtttccat tcgggaccgt tgccgccgcc gtccgacttg
     1861 gcgcaataca acgacattat cagcaacggg gcagaccgca ttatggcaat ggcggaaaaa
     1921 gaacaagccg tccggcacga aaccatacgg caagaccaaa ccttcaacag gcgcgggcaa
     1981 ctgtacggct tcatcagcgt catcctgata ctgctttttg ccgtcttcct cgtatggagc
     2041 ggctaccccg caaccgccgc ctcccttgcc ggcggcacag tggttgcctt ggcgggtgct
     2101 ttcgtgattg gaagaagccg agaccaaggc aaaaattaat gacaaatcct agggcgtgct
     2161 tcatatccgc ccgaacgccg aaccgcacat ataggcacat cccgtgcgcc gcccggaagc
     2221 ggaagccgcg ccctcccaaa caaacccgaa tcccgtcaga taaggaaaaa taatgaaaac
     2281 aaccgacaaa cggacaaccg aaacacaccg caaagccccg aaaaccggcc gcatccgctt
     2341 ctcgcctgct tacttagcca tatgcctgtc gttcggcatt cttccccaag cttgggcggg
     2401 acacacttat ttcggcatca actaccaata ctatcgcgac tttgccgaaa ataaaggcaa
     2461 gtttgcagtc ggggcgaaag atattgaggt ttacaacaaa aaaggggagt tggtcggcaa
     2521 atcaatgaca aaagccccga tgattgattt ttctgtggtg tcgcgtaacg gcgtggcggc
     2581 attggtgggc gatcaatata ttgtgagcgt ggcacataac ggcggctata acaacgttga
     2641 ttttggtgcg gaaggaagca atcccgatca acatcgtttt acttataaaa ttgtgaaacg
     2701 gaataattat aaagcaggga ctaacggcca tccttatggc ggcgattatc atatgccgcg
     2761 tttacataaa tttgtaaccg atgcagaacc tgttgaaatg accagttata tggatgggcg
     2821 gaaatatatc gatcaaaata attaccctga ccgtgttcgt attggggcag gcaggcaata
     2881 ttggcgatct gatgaagatg agcccaataa ccgcgaaagt tcatatcata ttgcaagtgc
     2941 gtattcttgg ctcgttggtg gcaatacctt tgcacaaaat ggatcaggtg gtggcacagt
     3001 caacttaggt agtgaaaaaa ttaaacatag cccatatggt tttttaccaa caggaggctc
     3061 atttggcgac agtggctcac caatgtttat ctatgatgcc caaaagcaaa agtggttaat
     3121 taatggggta ttgcaaacgg gcaaccccta tataggaaaa agcaatggct tccagctggt
     3181 tcgtaaagat tggttctatg atgaaatctt tgctggagat acccattcag tattctacga
     3241 accacgtcaa aatgggaaat actcttttca cgacaataat aatggcacag gaaaaatcga
     3301 cgccaaacat gaacacaatt ctctgcctaa tagattaaaa acacgaaccg ttcaattgtt
     3361 taatgtttct ttatccgaga cagcacaaga acctgtttat catgctgcag gtggtgtcaa
     3421 cagttatcga cccagactga ataatggaga aaatatttcc tttattgacg aaggaaaagg
     3481 cgaattgata cttaccagta acatcaacca aggtgctgga ggattatatt tccaaggaga
     3541 ttttacggtc tcgcctgaaa acaacgaaac gtggcaaggc gcgggcgttc atatcagtga
     3601 agacagtacc gttacttgga aagtcaatgg cgtggcaaac gaccgcctgt ccaaaatcgg
     3661 caaaggcacg ctgcacgttc aagccaaagg ggaaaaccaa ggctcgatca gcgtgggcga
     3721 cggtaaagtt attttagatc aacaagcaga tgaaaataat aaaaaacaag cctttagtga
     3781 aatcggcttg gtcagcggca ggggtacggt gcaactgaat gccgataatc agttcaaccc
     3841 cgacaaactc tatttcggct ttcgcggcgg acgtttggat ttgaacgggc attcactttc
     3901 gttccaccgt attcaaaata ccgatgaagg ggcgatgatt gtcaaccaca atcaagacaa
     3961 agaatccacc gttaccatta caggcaataa agatattgct acaaccggca ataacaacag
     4021 cttggatagc aaaaaagaaa ttgcctacaa cggttggttt ggcgagaaag atacgaccaa
     4081 aacgaacggg cggctcaacc ttgtttacca gcccgctgca gaagaccgca ccctgctgct
     4141 ttccggcgga acaaatttaa acggcaacat cacgcaaaca aacggcaaac tgtttttcag
     4201 cggcagacca acaccgcacg cctacaatca tttaaacgac cattggtcgc aaaaagaggg
     4261 cattcctcgc ggggaaatcg tgtgggacaa cgactggatc aaccgcacat ttaaagcgga
     4321 aaacttccaa attaaaggcg gacaggcggt ggtttcccgc aatgttgcca aagtgaaagg
     4381 cgattggcat ttgagcaatc acgcccaagc agtttttggt gtcgcaccgc atcaaagcca
     4441 cacaatctgt acacgttcgg actggacggg tctgacaaat tgtgtcgaaa aaaccattac
     4501 cgacgataaa gtgattgctt cattgaccaa taccgacatc agcggcaatg tcagccttgc
     4561 cgatcacgct catttaaatc tcacagggct tgccacactc aacggcaatc ttagtgcaaa
     4621 tggcgataca cgttatacag tcagccacaa cgccacccaa aacggcgacc ttagcctcgt
     4681 gggcaatgcc caagcaacat ttaatcaagc cacattaaac ggcaacacat cggcttcggg
     4741 caatgcttca tttaatctaa gcgaccacgc cgtacaaaac ggcagtctga cgctttccgg
     4801 caacgctaag gcaaacgtaa gccattccgc actcaacggt aatgtctccc tagccgataa
     4861 ggcagtattc cattttgaaa gcagccgctt taccggacaa atcagcggca gcaaggatac
     4921 ggcattacac ttaaaagaca gcgaatggac gctgccgtca ggcacggaat taggcaattt
     4981 aaaccttgac aacgccacca ttacactcaa ttccgcctat cgccacgatg cggcaggggc
     5041 gcaaaccggc agtgcgacag atgcgccgcg ccgccgttcg cgccgttccc tattatccgt
     5101 tacacctccg gcttcggtag aatcccgttt caacacgctg acggtaaacg gcaaattgaa
     5161 cggtcaggga acattccgct ttatgtcgga actcttcggc taccgcagcg acaaattgaa
     5221 gctggcggaa agttccgaag gcacttacac cttggcggtc aacaataccg gcaacgaacc
     5281 tgcaagcctc gaacaattga cggtagtgga aggaaaagac aacaaaccgc tgtccgaaaa
     5341 ccttaatttc accctgcaaa acgaacacgt cgatgccggc gcgtggcgtt accaactcat
     5401 ccgcaaagac ggcgagttcc gcctgcataa tccggtcaaa gaacaagagc tttccgacaa
     5461 actcggcaag gcagaagcca aaaaacaggc ggaaaaagac aacgcgcaaa gccttgacgc
     5521 gctgattgcg gccgggcgcg atgccgtcga aaagacagaa agcgttgccg aaccggcccg
     5581 gcaggcaggc ggggaaaatg tcggcattat gcaggcggag gaagagaaaa aacgggtgca
     5641 ggcggataaa gacaccgcct tggcgaaaca gcgcgaagcg gaaacccggc cggctaccac
     5701 cgccttcccc cgcgcccgcc gcgcccgccg ggatttgccg caactgcaac cccaaccgca
     5761 gccccaaccg cagcgcgacc tgatcagccg ttatgccaat agcggtttga gtgaattttc
     5821 cgccacgctc aacagcgttt tcgccgtaca ggacgaatta gaccgcgtat ttgccgaaga
     5881 ccgccgcaac gccgtttgga caagcggcat ccgggacacc aaacactacc gttcgcaaga
     5941 tttccgcgcc taccgccaac aaaccgacct gcgccaaatc ggtatgcaga aaaacctcgg
     6001 cagcgggcgc gtcggcatcc tgttttcgca caaccggacc gaaaacacct tcgacgacgg
     6061 catcggcaac tcggcacggc ttgcccacgg cgccgttttc gggcaatacg gcatcgacag
     6121 gttctacatc ggcatcagcg cgggcgcggg ttttagcagc ggcagtcttt cagacggcat
     6181 cggaagcaaa atccgccgcc gcgtgctgca ttacggcatt caggcacgat accgcgccgg
     6241 tttcggcgga ttcggcatcg aaccgcacat cggcgcaacg cgctatttcg tccaaaaagc
     6301 ggattaccgc tacgaaaacg tcaatatcgc cacccccggc cttgcgttca accgctaccg
     6361 cgcgggcatt aaggcagatt attcattcaa accggcgcaa cacatttcca tcacgcctta
     6421 tttgagcctg tcctataccg atgccgcttc gggcaaagtc cgaacgcgcg tcaataccgc
     6481 cgtattggct caggatttcg gcaaaacccg cagtgcggaa tggggcgtaa acgccgaaat
     6541 caaaggtttc acgctgtccc tccacgctgc cgccgccaaa ggcccgcaac tggaagcgca
     6601 acacagcgcg ggcatcaaat taggctaccg ctggtaacac cggatatgcc gaaaggggtt
     6661 tgacgatgcc gccgtgcgct gtcaaaccct ttttctgccg ccgccttgtg cctgctccat
     6721 tgtctgataa ccgtcaaatt gatttttagc ccatgtttgg tgggtcgggg aaatctatat
     6781 cttcgtgcgt gccgaaatag tctatgcccg atatacaatt ttgatacaca aacttggaaa
     6841 tatcggtatc gtcgccggag cgatagaatg cggacagttt ttcattgaat tgcggcatct
     6901 gcatttcgga gatttccaag atgccgcagc cccccgccat catcagaccg ttggcaaata
     6961 aggttgccgt tcgtttgttg ccgtcccaaa aaacctgctg ccgcatacaa taaagcataa
     7021 aacggacggc tgcctccgtc gtcgaaccgc tttgcagtcc gatattttgt aacacgcggg
     7081 ccacttcaat ttccttcact ggattcgggg catgacggga accgtccaat agcgttacgc
     7141 cgaccgaacc ggtacggaaa tcaccgggtg ccaaagaatc gtccttggca acaatgttgt
     7201 tgattttttt aaggagtgaa atattgaccg gttcgccatt tgaaatatgc gaaatcacat
     7261 attgataggc acgtttcagg ttcaagatgg tttggatgtc ttcaagtgac acggaggcta
     7321 catttttgcc atggataatt tgttcggtct gcaataaggt cgtctgacaa ttctcaaaac
     7381 ggctgaggtt gtgaatttgg gcaaccaata ctttctttgc caaaaaaata ttttctttca
     7441 aactcaactt gtatttgtcg ggaaacacga taattcctta aataattaaa gcagtacgtt
     7501 aaatcatcat cttatcatca ctttcggggt ttatgttgca tgcgtccgtt ttctaatacg
     7561 gtttcccatc ccgcgaaacg aggcggaggc ctgtgcaaaa aacctgttca cgaaattttc
     7621 cgcattaacg atacgctgaa aatgcgctaa aaatgactgt gtttgaatat cggttgattt
     7681 catccgtttg tgtaacctcg cgccggtttt gtcatgattt tgcattatag tgaattaaat
     7741 ttaaaccagt acagcgttgc ctcgccttgc cgtactatct gtactgtttg cggcttcgtc
     7801 gccttgtcct gatttttgtt aattcactat atttttgtca tgcggatatt tcacgggatg
     7861 acaaaacggg cgcaaaaaag cccgattgga aaatccgaat cgggcttttt tgtgatgttc
     7921 tgttcgattt gcgtactaac tgccggccgg agcgtccgcg cccggtttgg caggtcggat
     7981 tctcgaatcc gacggctatt tgagatggca ggcttttgtc ggatacaagt atccgaccta
     8041 cggctt
//
LOCUS       DAWKST010000090         8010 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.090, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000090 DAWKST010000000
VERSION     DAWKST010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8010
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.090"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            107..1048
                     /gene="miaA"
                     /locus_tag="WJL87_001347"
     CDS             107..1048
                     /gene="miaA"
                     /locus_tag="WJL87_001347"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ7799770.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYTGM
                     DIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLVGEISSRGRFALIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQQVDPETACRLKPNDSQ
                     RIGRALEVYYLTGKPMSTHLGSPTSHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRLYPSLTADSPAIRCVGYRQAWEYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVTDPFSDGNSCTRLIEAAKRFFGA"
     gene            1052..1771
                     /locus_tag="WJL87_001348"
     CDS             1052..1771
                     /locus_tag="WJL87_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family DNA transformation protein"
                     /protein_id="HEZ7799771.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGAVQTFLLLKASGLT
                     LTKSTLWQLQSLLDGTPPQEMAQHDKTRLLAELKNHPPVAAFPPQGEMAHFMGLALEQ
                     AKLSALMGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            1776..2264
                     /locus_tag="WJL87_001349"
     CDS             1776..2264
                     /locus_tag="WJL87_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEZ7799772.1"
                     /translation="MTALLVILALALIAAGTAGIVYPALPGLALMFAGTWLLAYSGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLIVGTTFKIGCAVSILFILLVKYIAY
                     LF"
     gene            2330..3082
                     /gene="rlmB"
                     /locus_tag="WJL87_001350"
     CDS             2330..3082
                     /gene="rlmB"
                     /locus_tag="WJL87_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ7799773.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYVQEGKNDVRTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVASGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGNADLYHCDLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
     gene            complement(3144..4535)
                     /locus_tag="WJL87_001351"
     CDS             complement(3144..4535)
                     /locus_tag="WJL87_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEZ7799774.1"
                     /translation="MSGHMGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            complement(4550..4804)
                     /locus_tag="WJL87_001352"
     CDS             complement(4550..4804)
                     /locus_tag="WJL87_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799775.1"
                     /translation="MLPIVAYFTAGVKSGGSGMHGAAWRRMRRVCIFVFEIRLLRFAN
                     DIPAATVRNLFAGRARMPSEIEIEAVWRIRYNPRFGFFNI"
     gene            4842..5717
                     /locus_tag="WJL87_001353"
     CDS             4842..5717
                     /locus_tag="WJL87_001353"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ7799776.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMTNDTILRLSEIPNIVGV
                     KEASGNVGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            5726..6922
                     /gene="bamC"
                     /locus_tag="WJL87_001354"
     CDS             5726..6922
                     /gene="bamC"
                     /locus_tag="WJL87_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ7799777.1"
                     /translation="MPSEAFRRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSHAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKKPTLPAAN
                     EMARIEGKSLIVLGDYGRNWRRTVLALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(6979..7911)
                     /gene="pip"
                     /locus_tag="WJL87_001355"
     CDS             complement(6979..7911)
                     /gene="pip"
                     /locus_tag="WJL87_001355"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ7799778.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIRHIPTIIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLVDALVQAVEDILPHLL"
BASE COUNT         2036 a         2636 c         1874 g         1464 t
ORIGIN      
        1 gcatacggta aacacgcccc cttgccgtcg gcgtgcttcc gcaaaccaaa atataacatc
       61 ggcacgcttt ccgctataat ccgcattttc gcaaccctga aacatcatgc cgaccccgaa
      121 agcctttgcc ctgctcgggc cgaccgccgg aggcaaaacc gcgctcgccc tcaaaattgc
      181 cgaaaccctg cctgtcgaaa tcatcagcct cgattccgcg ctggtctata ccggtatgga
      241 catcggcacg gcgaaaccgt ccgcctccga acgcgccttt gtcccgcacc accttatcga
      301 catcattacg cctgttcaga catacagtgc cgcccgtttt gtcgaagact gcacgcgctt
      361 ggtcggggaa atttcctcac gcggcaggtt tgcccttatt gtcggcggca caatgatgta
      421 tttccgcgcc ctgacccaag gtttgaacga tttgcccgaa gccgatgcct gcctgcgtgc
      481 cgacttggac gaacaaaaac aaatgtacgg cttggatttc ctctaccgca ccctgcaaca
      541 ggtcgaccct gaaacagcat gccgtctgaa accgaacgac agccagcgta tcggacgcgc
      601 tttggaagtt tattatttaa ccggcaaacc gatgagtacg catctcggca gcccaacctc
      661 ccatacgctc cccttcgatt tgcataccgc cgccctgatt cccgaaaacc gcgcccgcct
      721 gcatgaaaac atcgccctgc gtttccacct gatgcttgaa cagggcttta tcggcgaagt
      781 agaaaacctg cgccgcctct atcccagcct gaccgccgac tcccctgcca tacgctgcgt
      841 cggctaccgt caggcatggg aatatctgga cggaaaaacc gatttcccgg catttgtcga
      901 gaaaggcatt gccgccacgc gccaacttgc caaacgccaa ctgacttggt tgcgcaaaac
      961 acctttagac tgcgttaccg acccgttttc agacggcaat tcatgcacac gcctgattga
     1021 ggcggcaaaa cggtttttcg gcgcataaac tatgctgacc acgccgccgc tcgcccccaa
     1081 aaccgtcgcc gccctacaca ggctcggtat ccgtacgctc gaagaactgc ggcaaaacgg
     1141 cgcggttcaa accttcttgt tattgaaggc atcaggtttg accctcacga aaagcacgct
     1201 gtggcagctt cagtcgctgc tggacggcac gccgccgcaa gaaatggcgc aacacgataa
     1261 aacccgcctg cttgccgagt tgaaaaacca tccgcccgtc gccgcctttc ccccgcaagg
     1321 cgaaatggcg catttcatgg gtttggcatt ggaacaggcg aaactgtccg cattaatggg
     1381 cgaaataccc gtcggggcag tcatcgtttc agacggcaaa atcatcgcat cggcacacaa
     1441 cacctgcatt gccgactgca atgtcagccg gcacgccgaa atcaacgcct tggcacaggc
     1501 aggcagcgaa atgcaaaact accgccttga cggatgcgac atctacatca ccctcgaacc
     1561 atgcgccatg tgcgcgtccg cactgataca ggcacgaatc aggcgcgtga tctacggtgc
     1621 ggcagaaccc aaaaccggcg cggcaggaag catcgtcaac ctgtttgccg acaaacgcct
     1681 caatacgcac accgccatac ggggcggaat cctgcaagaa gaatgccgcg ccgtattaag
     1741 ccgttttttc caaaacaaaa gaaaaggttg aacctatgac cgccttgctc gtcatcctcg
     1801 ccctcgccct gatagccgcc ggtacggcgg gcatcgttta ccccgccctg cccggattgg
     1861 cattgatgtt tgccggaacc tggctgctcg cctactccgg cggctaccaa atctacggcg
     1921 cgggcgtttt gtggacggtc ggactcatca gccttgccgg catactggcg gactatgtgg
     1981 caggcatatg ggggacaaaa tataccggag cgggcaagct cgccgttcgc ggcgcattgg
     2041 ccggcagcat catcggcata tttttctccc ttcccggact aatactcggt ccctttatcg
     2101 gcgcggcggc aggcgaactg atcgatcggc gcaatatgct tcaggcaggt aaagcgggct
     2161 tgggtacgct gttggggctt atcgtcggta cgacattcaa aatcggctgc gccgtatcca
     2221 tcttgtttat cctgttggtg aaatacatcg cctacctgtt ttaacccctt cagacggcat
     2281 accggcaaat tgctataatg ccgtctgaac ccttttcaca aagcaccata tggcaaacca
     2341 aagacctatc tacggcttcc atgccgtcaa cgcccgattg tggcaaaacc ccaaatccat
     2401 cgtcgaactc tatgtccaag aaggcaaaaa cgatgtacgc acgcgcgaag tgttggaaaa
     2461 ggcggcaaac gaaaacatcc gcgtatattt tgccgatgcc gaccgcctca acgccatcag
     2521 caaaggtgcg cgccatcagg gcgtggtcgg atttatcgat gcctccaaaa accacgtcca
     2581 cctcgaagac gtattggaaa acttaagcga accgccgctg ctgctgatac tcgacggcat
     2641 caccgatccg cacaacctcg gcgcgtgcct gcgtaccgcc gacgcaatgg gcgtacatgc
     2701 cgtcatcgca ccgaaagaca aaagcgcggg gctgaacgcc accgtcagca aagtcgccag
     2761 cggcgcggcg gaaaccgtcc cctacatcac cgtaaccaac ctcgcccgca ccctgcgcga
     2821 gctgaaagaa tacggcattt ggatcatcgg caccgatatg ggcggcaacg ccgaccttta
     2881 ccattgcgat ctgcccgaca gcgcggcatg ggtgatgggc aacgaaggcg acggtatgcg
     2941 ccgcctgaca cgcgaacatt gcgacatgct ggtgtccata cccatgttcg gcacggtcga
     3001 aagcatgaac gtctccgtgt ctgccggaat ggtattaagc gaaacccgcc ggcagcgcgt
     3061 attaaaaaac gaaaaagcgt aagcctcaaa aacaatgccg tctgaaacag ggattctgtt
     3121 tcagacggca ttcgttttca aaatcagtgt tccaagtggt cggtatcaaa cttgaccgcc
     3181 gcctcggttt tatcttcggg caagaccaaa ttcagcaaga ctgccgtaat gccgccggcg
     3241 gaaatagagt tttggaacaa gacaggcagg tttttaaaca cttccggctc aaacgccacg
     3301 cccaagccca agccgaccga agtcgccgca atcaccgcct cgcgcctgcg gatgccgtgg
     3361 ctgaccaaaa tccgcacacc cgcaatcgca atcaagccga acatcaaaac catcgcgccg
     3421 cctaaaaccg gactcggaat cgtcgtaaac gcgcgtccga caacggggaa cagacccaac
     3481 agcaccaaaa tcacggcaat atatttgccc acatggcgcg aagccacgcc ggtcatctga
     3541 atcacgccgt tgttttgcgc aaacgttgtc agcggcagcg aacccaaagc cgtcgcaatc
     3601 accgacacca agccgtctgc caacacgccg ccgcgcaggc gtttggtgta ttcctcgcct
     3661 tcaatcggct gatcggacac cattgccgtt gcggtcaaat cgccgaccgc ctcaaacacg
     3721 ctcaacagga aaatcgcacc cgccacaata aatgcgtgcc agtcaaaagc aaaaccatat
     3781 ttaaacggta cgggcagcgt aaccagcggc aggttttgca gtgccgaaaa atccaccttg
     3841 cccaaaaaca gcgcgacgat atagccggca atcagaccga ccgcaatgcc gctcatccgc
     3901 agcagcgggt ttttcatgca gttgaacacc agcacaatca gcagcaccag cgatgccagc
     3961 cccaagtttt ccatcgagcc gaacgtgccg tccgcctttg cgccgaagcc gccgccgaaa
     4021 tcggtaatac cgacgtgtac caaactcaag ccgatcagca tcaccaccac accgctgacc
     4081 gtcggcgtaa tcactttttt caaatacggc agaagccacg ccgagaaaca caccaaaaac
     4141 gcgccgacaa acgatacgcc caagagcgtc gaaatcatcg catccttagt caaaccgccc
     4201 tctttcatcc ccgcgcccag cgcaatcatg acggtaacga acgagaaatt caccgactgg
     4261 atagacagca tccccgaacc gaccggcccg aagcggttga cctgcaaata agtgccgaca
     4321 cccgacgcaa ccatcgccat cgacacgaga tacgccgtca tctccaccgg cagttccagc
     4381 gcgccgccca caatcagcgc gggcgtaatc atcggcacga aaatcgccaa aagatgggta
     4441 accgcgctca acagcgcatt accgaacggc ggcctatcct ccaaaccgta caccaaatca
     4501 ggcgcatccg ccccttttcc catatgaccg ctcataatgt ttccttgctt caaatgttga
     4561 aaaaaccaaa gcgcggattg tagcgaatcc gccataccgc ttcaatttcg atttcggacg
     4621 gcatacgcgc ccgtccggca aacagattcc gaaccgtcgc cgccggaata tcgtttgcaa
     4681 aacgcaataa cctgatttca aacacaaaaa tacaaacccg acgcatccgc cgccaagccg
     4741 ccccgtgcat accggacccg ccggatttca cacctgcggt aaagtaggct acaatcggaa
     4801 gcattaccgc cccctacacc acaactgata aaaggttctg tatgttacaa ggtagcctgg
     4861 ttgccctgat taccccgatg aatcaagacg gcagcatcca ttacgaacaa ctccgcgact
     4921 taatcgactg gcacattgaa aacggcacgg acggcatcgt cgccgtcggc acgacaggcg
     4981 aatccgccac cctctccgtc gaagaacaca ccgccgttat cgaagccgtc gtcaaacacg
     5041 ttgccaaacg cgtccccgtc atcgccggca caggggcgaa caacaccgtc gaagccatcg
     5101 ccctttccca agccgccgaa aaagccggcg cggactacac cctctccgtc gtcccctatt
     5161 acaacaaacc ttcgcaagaa ggcatgtacc gccatttcaa agccgttgcc gaagccgccg
     5221 cgattccgat gatactctac aacgttcccg gccgtaccgt cgtcagcatg actaacgaca
     5281 ccatcctgcg cctttcagaa atccccaata tcgtgggcgt taaagaagcc agcggcaacg
     5341 tcggcagcaa catcgaactc atcaaccgcg cccccgaagg cttcgtcgtc ctgtccggcg
     5401 acgaccacac cgccctaccg ttcatgctct gcggcggaca cggcgtgatt accgtagccg
     5461 ccaacgccgc gcccaagctc tttgccgata tgtgccgcgc cgcgcttcaa ggcgatatcg
     5521 cccttgcacg cgaactcaac gaccggctga ttccgattta cgacaccatg ttctgcgaac
     5581 ccagccccgc cgcaccgaaa tgggcggtat ccgccctggg cagatgcgaa ccgcacgtcc
     5641 gcctgccgct cgttccgctg acggaaggcg gacaggcaaa agtccgcgcc gccctgaaag
     5701 cctcaggaca actttaaata tagccatgcc gtctgaagcg ttcagacggc ataacgcaac
     5761 aaacacttta atatccatca cacaggatga cacgatgacc catatcaaac ccgtcattgc
     5821 cgcgctcgca ctcatcgggc ttgccgcctg ctccggcagc aaaaccgaac agcccaagct
     5881 cgactaccaa agccggtcgc accgcctgat caaactcgaa gtcccacctg atttgaacaa
     5941 ccccgaccaa ggcaacctct accgcctgcc tgccggttcg ggcgccgtcc gcgccagcga
     6001 tttggaaaaa cgccgcacac ccgccgtcca acagcctgcc gatgccgaag tattgaaaag
     6061 cgtcaaaggt gtccgcctcg agcgcgacgg cagccaacgc tggctcgttg tcgacggcaa
     6121 gtctcatgcc gaaatctggc cgctcctgaa agccttttgg caggaaaacg gcttcgacat
     6181 caaatccgaa gaacccgcca tcggacaaat ggaaaccgag tgggcggaaa accgtgccaa
     6241 aatcccccaa gacagcttgc gccgcctatt cgacacagtc ggtttgggcg gcatctactc
     6301 caccggcgag cgcgacaaat tcatcgtccg tatcgaacag ggcaaaaacg gcgtttccga
     6361 catcttcttc gcccacaaag ccatgaaaga agtgtacggc ggcaaagaca aagacacgac
     6421 cgtatggcag ccctccccgt ccgatcccaa cctcgaagcc gctttcctga cgcgctttat
     6481 gcaatatttg ggcgttgacg gacagcaggc ggaaaacgca tcggcaaaaa aacctaccct
     6541 tcccgccgcc aacgaaatgg cgcgtatcga aggcaaaagc ctgattgtct taggcgacta
     6601 cggcagaaac tggcggcgca ccgtgctcgc cctcgaccgc atcgggctga ccgtcgtcgg
     6661 tcaaaacacc gaacgccacg ctttcctggt tcaaaaagcc ccgaacgaaa gcaatgcagt
     6721 taccgaacaa aaacccggcc tgttcaaacg cctgctgggc aaaggcaaag cggagaaacc
     6781 tgccgaacag ccggaactga ttgtctatgc cgagcctgtc gccaacggct cgcgcatcgt
     6841 cctgctcaac aaagacggca gcgcatatgc cggcaaagac gcatccgcat tattgggcaa
     6901 actccattcc gaactgcgtt aaaccattca aaacaaaagc agcttccaaa cagaagctgc
     6961 ttttttatgc ggaacttttt acaacaaatg gggcaaaata tcctcaactg cctgaaccaa
     7021 cgcatccacc aaaggcggat cgaacgcacg atgccctgcc tgaaccaccc tcaattccgc
     7081 ttcgggaaag gctttcgaca gcgcccacgc actctgcatc ggcgtacaca aatcataccg
     7141 cccctgcacg ataatggtcg ggatatgccg tattttgccg atattgttca aaatcgccct
     7201 atcgccctgc aaccagccgc cgttgacaaa ataatggttt tccaaacgcg cgattgccag
     7261 cgaggcataa gcatcttcat ccacttcctc cggctcgaaa cggatcagat agctttccca
     7321 atccgcccaa gcctttgcgg cagacaggca cacttcttca tcttgatgaa acagcaaccc
     7381 gtgatacgcc tcaatcagcc ggttccgccg attttcagca atcggcgcga caaatttttg
     7441 ccattgttcc ggataaatcc ggctcacacc gcccgcctcg ttcagccacg ccgtttcaga
     7501 cggcctgcac aaaaatatcc cgcgcaacac caatcccttt acccgttccg gatgggtttg
     7561 ggcataagcc agcgacaaag tactgcccca cgaaccgccg aacaccagcc atttcccgat
     7621 acccagcatt tcacggactt tttcaatatc cgccaccaaa tcccaagtcg tattgtcttc
     7681 cgcacaagca tacgggcgcg aacgtccgca accgcgctgg tcaataatga cgatgcggaa
     7741 cacatcggga ttgaaaaaac cccgacattc aggcgaagcc cccgcccccg gtccgccgtg
     7801 taaaaaaatg accggcacac cgtcgggatt gcccgactct tcccaataaa tttgatgaat
     7861 ttcagacacc tgcaagtatc cgctatgaaa aggctgtttt atctcataca tcatcttctc
     7921 ccatattgcc tctgacaatc cgaacattcc cacacgcaac atcacgcacg aaacacaagc
     7981 cgccccgaaa ggcaaattcc gaaaaatcgc
//
LOCUS       DAWKST010000091         7861 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.091, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000091 DAWKST010000000
VERSION     DAWKST010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7861
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.091"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(19..2061)
                     /gene="recG"
                     /locus_tag="WJL87_001356"
     CDS             complement(19..2061)
                     /gene="recG"
                     /locus_tag="WJL87_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEZ7799779.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTFPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALCQALPFALTDAQEKVVSEICRDM
                     AQTHPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLVDGSVKIAVGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDKLPPGRTPIKTRL
                     VNNVRRAEVEGFVLGACRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGGLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQAREIAPILIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(2147..2590)
                     /gene="secB"
                     /locus_tag="WJL87_001357"
     CDS             complement(2147..2590)
                     /gene="secB"
                     /locus_tag="WJL87_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ7799780.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(2611..2868)
                     /gene="grxC"
                     /locus_tag="WJL87_001358"
     CDS             complement(2611..2868)
                     /gene="grxC"
                     /locus_tag="WJL87_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ7799781.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            3081..4574
                     /gene="rng"
                     /locus_tag="WJL87_001359"
     CDS             3081..4574
                     /gene="rng"
                     /locus_tag="WJL87_001359"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ7799782.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(4608..5858)
                     /locus_tag="WJL87_001360"
     CDS             complement(4608..5858)
                     /locus_tag="WJL87_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ7799783.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRILVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTEQGRIAV
                     MQENEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQPAVLQQSIRRNKHLLEQLLALARSQSDETPLTKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVRGDVEVSADETEIYTLIKTFADNAVRYTPPEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERARVLDPFYRILGMEQQGTGLGLSIADTLATEYGRYL
                     ELSDSRRFGRGLLIRALLDKESLK"
     gene            complement(5854..6029)
                     /locus_tag="WJL87_001361"
                     /pseudo
     CDS             complement(5854..6029)
                     /locus_tag="WJL87_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(6252..7565)
                     /locus_tag="WJL87_001362"
     CDS             complement(6252..7565)
                     /locus_tag="WJL87_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ7799784.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDLVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
BASE COUNT         1849 a         2324 c         2051 g         1637 t
ORIGIN      
        1 tttcagacgg cattttgctc acacgcccaa atacccttcc ctgccggaaa gccaccttgc
       61 caaatgcgct tcgacgattt cggggttttg ctcaatcagt atcggggcga tttcgcgcgc
      121 ttgttccaaa aggtgcaggt cttcttcgag tttggcgaaa cgcagcatag gcacgccgct
      181 ttggcgcgcg ccgagaaact cgcccgggcc gcggatgttg aggtcttggc gggcgatttc
      241 gaagccgtcg gtgtgttcgt agatgacttt cagccgcgct ttggcgagtt cgcccaaggg
      301 ttcggcaaac aggaggacgc acacgctttc cgccgcgccg cgcccgaccc gaccgcgtaa
      361 ttggtgaagc tgcgccaagc ccatgcgctc ggcgtgttcg atgaccatca gggcggcatt
      421 gggcacatct acgccgactt cgatgacggt ggtggcgacc aagacgttca gcccccccga
      481 agaaaaccgc gccatcactt cggccttttc ggcggccttc atgcgcccgt gtaccagtcc
      541 gatattgagt tcgggcaatg ccgtctgaag ccgggcgagg gtttcggcgg cggtttgcag
      601 ttgcagggtt tcgctttctt caatcaatgg gcagacccaa tacgcctgcc gcccttttcg
      661 gcaagcgccg aggacgaagc cttcgacttc ggcgcggcgg acgttgttga cgaggcgcgt
      721 tttaatcggt gtgcgcccgg gcggcagttt gtcgataacg gacacgtcca aatcggcgaa
      781 aaaactcatc gcaagcgtgc gcgggatggg cgtggcggac atcatcagct gatggacttc
      841 gcgccctttg tttttgaggg cgaggcgttg ggcaacgccg aaacggtgct gttcgtccac
      901 aatggtcaag cccaaattgt gaaacgccac gccgtctgaa aacagggcgt gcgtgccgac
      961 ggcgattttg acgctgccgt cgacgagttt ggctttggct tcgtctttgg cttttttacg
     1021 caaactgcca aaaaggcgga caacttcaat acccaaaggt tcgagccatt gtttaaattt
     1081 aataaaatgc tgttcggcaa ggatttcggt cggtgccatt acagccacct gcgcgccgga
     1141 ttcgatagcc gtcaaagcag acaaagcagc cacaatggtt ttgccgcttc cgacatcgcc
     1201 ttgcagcagg cggtgcatgg gatgggtttg tgccatgtcg cggcagattt cggaaacaac
     1261 tttttcttgc gcatcggtca gggcaaacgg caaggcttgg cacagggctt gggtcaatgt
     1321 gccgtcgccg cccaatgccg ccgccgtgcc gccgatacgc ttctgccgcg ccaagcgcat
     1381 ggaaagctgt tgtgccaaaa gttcgtcgaa tttgagccgt tgccatgcag gaaacgtgcc
     1441 gtctgaaagt tgatgaatgg tgaaactcgg cggcggcgaa tgcaaaaggc gcaggctttc
     1501 ggcaaggtgc ggcagcttca gacggcacag cagggcatcg ggcagcgtgt cgtgcagcgg
     1561 cgtaacgtcc aacgccgtct gaataatacg gcgcaaagtg ggctggttca aaccgtttac
     1621 ggtcgggtaa accggcgtga ggctttccgc caaaccgccg ccctcggcat cgcggatttt
     1681 gggatgaatc atctcgtcgc cgtaaaagcc gtgtttgatt tcgcccacgg cgcggatgcg
     1741 tttgccgacc gccgtctgtt tctgatggct ggcgtaaaag tggatgaagc gcagaaaaag
     1801 gacgctgccg gaaccgtcgg cgatttggac aatcaactgc ttgcgcggtt tgaacgttac
     1861 ttcctgatgg ataacctcgc cctcgacctg gcacggcacg ccaatcggcg cgtccttaat
     1921 cggcataatg tgcgtctcgt cctcgtaacg cagcggcagg tgcaacacca aatcccacgc
     1981 ggtatggagg ttgagtttgt cgagcttctt ggcggaaaca tcggtgattt tgagctgttt
     2041 ctgggtttcg ggcgacatca taggcagatt cctttgaacg cacctatttt atccgaaaac
     2101 aaaaataccg tctgaaacgg attcagacgg catcggcagg caggaatcaa gccccggcgg
     2161 cttcggcttc ctgctgttgt tggtaaatcg cctcaaaatt aatcggcgcg agcaggacgg
     2221 gcgggaagcc ggcacgtgtt accgtaccgg aaacggcttc gcgcgcgtaa gggaagagga
     2281 tgttcggaca cgccacgccc agcagcagct gcacatcttc ttcggggatg ttttccaaac
     2341 ggaaaatacc gctttgggtt acttcgttca aaaacatcgt gcgctcgtta tccaatttgg
     2401 cggttacggt aacggttaca tccacgttgt agtagccgtc ttccagcttt tggctgccgg
     2461 tggaaacgcg catatccact tcgggctcgc cctgttccaa aaagatttgc ggcgcgtgcg
     2521 gcacttccaa agacaagtct ttgacataca gtcgctcgat gctgaatacg ggttgcagtt
     2581 cttcgctcat tttgttttcc tagttggggg ttaagggttc agcagtccgt ccagcccgcc
     2641 ttcctgctgg aggcggtaga ggtcggtaaa tccgccgacg tgcgtttcgc cgatgaaaat
     2701 ctgcggcacg ctgcgctgtc ccgaaagctg ctgcatttcg gcaaaggctt cggggcttgc
     2761 atcgacacgg atttcgtcga tatgtccgac acctgccgcg tgcagcagcc ttttcgccat
     2821 cgcgcagtag ggacaaaacg gacctgtgta catggtaacg gtctgcatat tgggtttccg
     2881 aaagttttgc aatgataatc aatatagggg catttcccct gtttggcaag tgcggaacag
     2941 atgcacgttc aaacggcatg tgcggaatgt gtcaaagttt cttttttaaa gtatgataga
     3001 cattgtgaaa aatatttttg cacccgcgct gcgcggcgga acggatgcaa aatattttta
     3061 ttacattttc aggaaaaacc atgttgtcag gactccccat ccccaaagac atcgcgcgcc
     3121 cgcccgaaac gatattggtc aacatcacgc cgcaggaaac gcgcgtagcg gtgttggagg
     3181 aaaacaatat ctgcgagctg cacatcgagc gcaacagcga acacagccta gtcggcaata
     3241 tctatttggg cgtggtgcgc cgcgtgctgc ctgggatgca gagcgcgttt atcgacatcg
     3301 gcttggaacg cgcggcgttt ttacacatcg tcgatgtcct cgaacaacgc cgcaaccccg
     3361 aagaaaccca gcgcatcgaa catatgctgt ttgaagggca gtctgttttg gtgcaggtca
     3421 tcaaagaccc gatcaacacc aaaggcgcgc ggctttccac ccaaatctcg ctggcggggc
     3481 gtttcctcgt ccatcttccg caagaagacc acatcggcgt gtcccaacgc atcgaagacg
     3541 atgccgaacg cagcagcctg cgcgaacgcc tcgacaagct cctgccggaa aatgcctgcc
     3601 ggggctacat catccgcacc aacgccgaaa acgccaccga cgaacagctc cagtccgaca
     3661 tcgactacct gaccaaagtg tgggaacaca tccaagaaca ggcgaaaatc cggccgccgg
     3721 aaaccctgct ttatcaggat ttgcccttga gtttgcgcgt gttgcgcgat atggtcggct
     3781 gcgacacgca aaaaatcctc gtcgattcca ccgtaaacca cggccgcatg acgcgttttg
     3841 ccgaacaata cgtccacggc gcattgggca ggatagaatt gttcaaaggc gaacgcccgc
     3901 tatttgaaac ccacaacgtc gagcaggaaa tcagccgcgc cctgcaaccg cgcgtcaacc
     3961 tcaacttcgg cagctacctg attatcgaat ccaccgaagc gatgaccacg atagacgtga
     4021 acaccggcgg cttcgtcggc gcacgcaatt tcgatgaaac gattttccgc accaacctcg
     4081 aagcctgcca caccatcgcc cgcgaattga ggctgcgcaa cctcggcggc atcatcatca
     4141 tcgacttcat cgatatggcg caggaaagcc accgcgaagc cgtgttgcag gagcttgcca
     4201 aagccctcgc cttcgaccgt acccgcgtta ccctgcacgg ttttaccagc ctgggactgg
     4261 tcgagctgac gcgcaaacgc tcgcgcgaaa acttaaacca agtcctctgc gaaccctgcc
     4321 cttcctgcca aggcagaggc cgtctgaaaa cgccgcaaac cgtatgctac gaaatccagc
     4381 gcgaaatcgt ccgcgaagcg cgccgttacg atgccgaaag tttccgcatc ctcgccgccc
     4441 ccaacgtcat cgatttgttt ttggacgaag aatcgcaatc cttggcaatg ctgatagatt
     4501 tcatcggcaa accgatttct ctggcagtcg aaaccgctta cacgcaggaa caatacgaca
     4561 tcgttttgat gtaaaaatgc cgtctgaagc cttcagacgg catctgtcta tttcagggat
     4621 tccttgtcca acaaggcgcg tatcagcagc ccgcgcccaa accggcggct gtcggacaat
     4681 tccaaatatc tgccgtattc tgtggcaagc gtgtcggcga tggacagccc cagccccgtc
     4741 ccctgctgct ccattcccaa aatacggtaa aacggatcga ggacgcgggc gcgttcggat
     4801 tcgggaatgc cgttcccgtt atcttccacc cacacggcaa gatatttccc ttcgtccgtg
     4861 aaacccaaat ctatcctgcc ttcaggcggc gtgtaacgta ccgcgttatc ggcaaaggtt
     4921 ttaatcagcg tatagatttc tgtttcgtcg gcagacactt cgacatcgcc tctgaccgcc
     4981 acaccgatgt cctgacgttt ttccaaagcc agcggcatca gttcctgcaa cacttggcgg
     5041 aaacggcttt gcagaccgaa tgtcgttttc gtcagagggg tttcgtccga ctgcgaacgc
     5101 gccagtgcca aaagctgttc gagcaggtgt ttgttgcgcc tgatgctctg ctgcaaaacg
     5161 gcaggctgcc gcgctgcatc gggtgggagc gacatattgt tgagccgttc cgcctgaagg
     5221 gaaagggctg tcatcggcgt acgcaattcg tgtgccgcgt cggcgacaaa ccgctgacgg
     5281 tggcggatgt cttcatccgc acgtttcaaa agcaagttga tggcggttac gaaccctctg
     5341 atttcactgg gaatattgtc cacactcaag gcagacaggt cattgattcg gcgttgttcg
     5401 agactttgcg acaatttgcg gacggggcgc atggctttgt gcgtaatcca cacggtcagc
     5461 agtatcatca gcggcagtgc cgccagcagg ggcaacacgc tttgccgtgc cgcatccgcc
     5521 gccaaatctt cacggtattc gttttcctgc ataacggcaa tccgtccctg ttcggtcgtg
     5581 cggatataga cgcggtaata atcgtcgtcc ccgtccgcct gaagcgtgtg cagaccgtcc
     5641 gccagatgcg caggcaggct gacaacaggg tcttcctgct gcggcatctg taccaaaata
     5701 cgcgtatcgc cgtcgccctc gggcaaagtt tcgggtttgg aatcgggggc gacgtacaat
     5761 gccgcctgac ggagcaggtc gtcctgcaac gcttccgttt cgtggaaggt ttcgtagtag
     5821 gaaaacatac ctgcaagcat tgccagcgga acaaacatcc aaaccgtccc gccccggcaa
     5881 acccaaatcc agcagcatca agtcataagg ctgggcagcg gcaaccgcgc gccgtttttg
     5941 acccaatcca ccgcatagcc gccgtctttc aaacttgccg acaccgcctc cgcaatcatc
     6001 gcatcgtctt ccaccagcaa aacacgcatc aactttccct tcaaaataaa ccgtgcctat
     6061 tctaacaccc caaaattagc cgcaatttag cggtctttac gcttgccggt atttttcaaa
     6121 actgcagcac aaaaaaaccg cgccggcaac tgccttcaga cggcattggg gcgcgattgc
     6181 aacacacggg caggcaggca gagcctgcga cagaccacag gaacgattcg ggcttcagac
     6241 ggcttcgccg tttacggcag aggcacgatt cctgccgcta tcgaactggc caatatcgcc
     6301 agcgacaaac cccacgccca gaaaaacgaa taacggatgt gtttgcccat cgacaatttc
     6361 gccaaaccca agcccatcca caaagccggc gaaagcggcg taacaaaagt gccgacgata
     6421 ctgccgatca acatcgcata acctgctgct tcaggcgcca cgcccgcctg cgaggtaatc
     6481 tgctccacaa tcggaaacag tccgaaataa taagcgtccg tactcaaaac caactcaagc
     6541 ggaatgccca acacaccgat ggcaatatgc agataaggca gcagcgcgtc cggcaggata
     6601 tgcacaaggt ctttggaaat cgcgtccaac atccccgcac ccttcaaaat ccccaaaaac
     6661 gtacctgccg ccaaaataat ggacgccatc atcaccgcgc cgccggcgtg ggcataaatc
     6721 cgctccatct gttcctgcgg gctgcggtaa ttcaaaagca acgccgccgt tgcagccagc
     6781 ataaatacat aacccggcgg gaagatgccc gaaaaaagca ggctcatcgc cgccaaaaac
     6841 agcaggacat tccaccaaaa cagtttcgga cgcgccaatt tttgttcttc ttccgacaaa
     6901 ggcaccggct ttatcaaatc cgccacggcg ggcaacgcgc ccaactcccg gacaatccgc
     6961 cttttttcac gcacacccaa aagcagggac agcgcaagga taaacactac accgataatt
     7021 tgcaccgtca acaaaggttt atacaattcg cccacatctg cgcccaacac gcttgcaacc
     7081 cgcccagtcg gcccgcccca cggcagaagg ttaatcaatc ccgcactgga agtcagcagc
     7141 aaaaacagca ggtaaggatt catatgcaga cgcttgtaaa gcggcaaaag ggcggggacg
     7201 accaataaaa acgtcgtcgc acccgccccg tccaactgcg ccaccaccga caccaagacc
     7261 gtccccacac tcactgccac gatattaccc cgagtcagct taatcaaacc gcctatcatc
     7321 ggacggaaca gccccacatc gttcatgatt ccaaaaaaca aaatggaaaa cataaacata
     7381 atcacaatct gcatcaccga tttggtgccg cccgaataaa attcttttaa ttgggataca
     7441 tcaaaccccg ccagcaacgc cccaaacagc ggcaccaaga ttaatgcgat gatgggcgac
     7501 actttttccg tcagcagcaa ccatacgatg accccgataa tcagcagtcc gataaacgtc
     7561 agcatcattt ctcctttatt ttattttaaa cagaaaaccg accgtgcagg caaaaccgcc
     7621 cacagacgcg ggataagcct tgcattctac ttttttattt tgaaacaagt caatcggtca
     7681 tttcctccca tttacgcctg ccgccattcc tgtatccgtc cgtcatttca cggcggcaac
     7741 cgatacggaa caaccggcaa accggtatcg ggacggcgcg ggggcattca tcccggtgcg
     7801 ccgattcaaa cgaaaccgcc cctatcattg cggagcgcgg ggcgtgccgt acacgcggga
     7861 t
//
LOCUS       DAWKST010000092         7719 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.092, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000092 DAWKST010000000
VERSION     DAWKST010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7719
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.092"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            252..1787
                     /locus_tag="WJL87_001363"
     CDS             252..1787
                     /locus_tag="WJL87_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ7799785.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            1784..1876
                     /locus_tag="WJL87_001364"
     CDS             1784..1876
                     /locus_tag="WJL87_001364"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ7799786.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1936..3156)
                     /gene="lysA"
                     /locus_tag="WJL87_001365"
     CDS             complement(1936..3156)
                     /gene="lysA"
                     /locus_tag="WJL87_001365"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ7799787.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQDEDVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGS
                     LLTRVEFVKYGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKDIATLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVNGGGYKLIRRR
                     ETLEQQMANELACL"
     gene            complement(3167..3337)
                     /locus_tag="WJL87_001366"
     CDS             complement(3167..3337)
                     /locus_tag="WJL87_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ7799788.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QGKPQSAPPTQK"
     gene            3408..3731
                     /gene="cyaY"
                     /locus_tag="WJL87_001367"
     CDS             3408..3731
                     /gene="cyaY"
                     /locus_tag="WJL87_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ7799789.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     EIEEL"
     gene            3762..4181
                     /locus_tag="WJL87_001368"
     CDS             3762..4181
                     /locus_tag="WJL87_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HEZ7799790.1"
                     /translation="MAQLPLYRTAEIGNFTVGTPKVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALCVNDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSREPT"
     gene            4203..4709
                     /gene="luxS"
                     /locus_tag="WJL87_001369"
     CDS             4203..4709
                     /gene="luxS"
                     /locus_tag="WJL87_001369"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ7799791.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCIPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVGNVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            4855..7647
                     /gene="polA"
                     /locus_tag="WJL87_001370"
     CDS             4855..7647
                     /gene="polA"
                     /locus_tag="WJL87_001370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ7799792.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVMEHAS
                     EIKGKVGENLRAALPRLPLSYDLVTIKTDVDLHAELSDGIESLRRTAPKWAQLVVDFK
                     RWGFRTWLKEAESNMNTGSTDDVFGSDSIGEQAALNAEMPSEKQAEKATAPERLDYQA
                     VTTEAQFAALLDKLSRANTIGIDTETTSLDAMNASLVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLGNPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQIGFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDEKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QQAYAAADQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPKDGRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVRNCLSDGIGSKLVMQVHDELVLEVVETELDFVKEKLPQIMAKVDGGLLDV
                     PLVAEVGVGENWEEAH"
BASE COUNT         1778 a         2077 c         2105 g         1759 t
ORIGIN      
        1 cggcagctaa aaatcctaca ggattggtgt gggatttagg gctaatctag tacagcccct
       61 aaaatttttc gttttcaagc cttcaccgct tgccatcagc gttaaatttt tttacgataa
      121 gcacatagat tgtaaacaat cggccacaag ccggtttgtt ttttcagaag acattatccc
      181 tgtcagacgc tatttctata tatttcgcct ataacggctt gtttttaata aataattcaa
      241 gaggtatcaa cgtgtctgat tccaaaacga aagaacgcgc cacattcggc acgcgccgcg
      301 cgtttatgat tgccgccatc ggatccgccg tcggcttggg caatatttgg cgtttcccct
      361 atattgcttt tgaaaacggc ggcggcgcgt tcatcctgcc ctatctggtc gcgcttctga
      421 cggcgggcat tccgctgctg ctgctcgatt atgccatcgg ccaccgttac cgtggttctg
      481 cgcccttggc tttccgccgc ctcggacgct ggtttgagcc ggtcggctgg tggaacgtga
      541 tgaccaatat cgtcatctgc atctattacg cggtaattat cggttgggcg gcaagctata
      601 cctattattc ggtcaacgcc gcctggggtg cggatccgca gggttttttc tttaaagact
      661 tcctgcaaat ggcgggcccg gaagccttgg ggttggattt tgtcggcaaa gtggccggtc
      721 ctttggcggg cgtgtgggtt tttaccgccg ccattatggc tttgggcgtg caaaagggcg
      781 tggcgcgcgc ctcgtcgttc tttatgccgc tgcttttggt gatgtttttg attatggtcg
      841 gcatttcgct gacgttgccg ggcgcggcaa agggcttgga cgcattgttt acgcccgact
      901 ggtcgaaact cgccgattcc aaggtctggg tggcggcata cgggcagatt ttcttttcgc
      961 tttccatctg cttcggcatt atggttacct attcttctta tttgaagaaa aaaaccgact
     1021 tgggcggaac ggggctggtg gtcggttttg ccaacagcag ctttgaactg ctcgcgggca
     1081 tcggcgtgtt tgccgcattg ggctttatgg cgcaggcggg cggtaaggcg gtcaacgagg
     1141 ttgcctcagg cggcatcggt ttggcgttta tcgcctttcc gaccattatc aaccaggcac
     1201 cgatgggctg gctgatcggc atattgtttt tcggttcgct ggtattcgcc ggcgttacgt
     1261 cgatgatttc catccttgaa gtgattgtgg cggcgattca ggacaagctg aacatcgggc
     1321 gcgtcaacgc cacgctgctg gtctgcattc cgatgggcat tgtttccacg ctgctgttcg
     1381 gtacggcgac ggggctgccg gttttggacg tgatggacaa attcgtcaac acctacggca
     1441 ttgttgccgc cggctttgtt tatgttgccg ccatcatcat cagcggcagg ctgccggaat
     1501 tacgcaagca cctgaacgct ttgtcctcca tccgcatcgg cggcttgtgg acggtctgcg
     1561 tcgtggttac cgtcgtgatg ctcggctata tgctgtttaa agataccagc ggcctgatgg
     1621 agaaaaatta cgaaggttat ccggatggtt tcctcagtat tttcggctgg gggatgtcgg
     1681 cggcgttggt cgtgttcggg ctgctgctgt cgttgctgcc ttggaaacac ggtcaggatt
     1741 tcaacgtcaa agacgaacac gaacatgaac aaggagaaga aaaatgagta cttccgccat
     1801 tgtgatgatg attgtctcaa tcgtgataat ctggggaggg ctgctgcttt ccctgttaag
     1861 gctgccgaac gagtaagcct ttagagcgtt aaaaatgccg tctgaactgt ttcagacggc
     1921 attttgatgt tcggcttaca ggcaggcgag ttcgtttgcc atttgctgtt ctaaggtttc
     1981 gcgtcggcgg atgagtttgt atccgccacc gttaaccaac acttccgccg cacggttgcg
     2041 cgcgttgtaa ttgctcgcca tactggcccc gtatgcgccc gcgctgcgga taagcagcaa
     2101 atccccttct tcgcaggcga tggtgcggtc tttgccgagg aagtcgccgg tttcgcaaat
     2161 cggaccgacg atgttggcgg tcagcgtcgc gatgtctttg gtttcgaccg cctcgatgtg
     2221 atgataggca tcataaagcg ccgggcgcat caaatcgttc atcgccgcat cgaccatcac
     2281 aaagtttttc tcttcgccgt atttgacaaa ctcgacgcgt gtcagcagcg aacctgcgtt
     2341 gccgaccagg ctgcggccgg gctcaagaat gagtttcaga cggcgtgtgc cgatcagttt
     2401 ttgaacggct tgggcatacg cgcccaaatc aggcacatct tcgtcttggt aaacaatgcc
     2461 gacgccgccg cccaagtcca agtgttccaa cacaatgcct tcggcggcaa gcgcgtcaac
     2521 caaaatcaaa atgcgctcgc aggcttcgac aagcggactt aagtcggtta gttgggagcc
     2581 gatgtggcag tcgatgccga tgattttcaa attgggctgt tgtgcggcgt ggcggtaggc
     2641 ttcgagcgcg tcggcgtagg cgatgccgaa tttgttggct ttcagacctg tggagatgta
     2701 gggatgggtt ttcgcatcga catcgggatt gacgcgcagg gagacgggcg cggttttacc
     2761 caaacgcgcg gcaactttct gaatacggtc gatttcgggg atgctttcca tattgaagca
     2821 tttcacgcct gcattcagcg cgaactcgat ttccgcctcg cttttgccta cgcctgaaaa
     2881 tatggttttt gccgcgtcgc cgcctgccgc caaaacgcgt gccaattcgc cgccggacac
     2941 aatgtcaaaa ccgctcccca aagaagcaaa gtgtttgatg atgctcaggt tgccattggc
     3001 tttaaccgca tagcagacga gcggggagag ctcggcaaag gcagtttggt agtgttcaaa
     3061 tgcttcggtc agcgcggatt ggctgtacac ataaagcggt gtgccgaatg cttcggcaag
     3121 gcgggggtag gggacttgtt cgcaaaatag ggtcatattt tcgttttcat ttttgggttg
     3181 gaggggcgga ttgcggtttg ccttgaagct gcaaaccggt ttggattacg ccgaaacgcg
     3241 ccttgtcgcc ttctttgggc aggtagaggt cgcctttgta accgcaggcc gagagcagga
     3301 gggcggttgc cgccgcaaaa aatacgccgt atttcatcgg taaacttcct tcataagcgc
     3361 gaatgtggca agattcggca tcttaaacaa aaaacacgca aaaagctatg atgaccgaaa
     3421 gcgagtttat ccgcgcgagc gaagcattat ttgaacacat cgaagaccaa atcgacgaaa
     3481 acggctggga tttcgactgc cggtttgccg gaaacgtcct gaccatcgaa gccggagacg
     3541 gcacgcaaat catcgtcaac cgccacacgc ccaatcagga attgtggatt gccgcaaaaa
     3601 gcggcggcta ccatttcgcc gaacaaaacg gcaaatggct ggcaacgcgc gacagccgcg
     3661 atttttatga tgttttgaac gaagcactga gcgcggcttc gggcgaagcg gttgagattg
     3721 aggaattgtg attcaatttg atataaaaag attgttaccc aatggcacaa ttacctctat
     3781 atcggactgc cgaaatcgga aattttactg tcggcacgcc taaagtttta gaatcatttt
     3841 ccaaacatat cccttatggt gtcgtctttg aagacgacgg cgacacgggc tacttctatg
     3901 ctgcttcgca agacgggatt ttagatgcct tgcacatcta caatgtcgaa gatgtatccg
     3961 acaaacatat ccccaatcat gtcttgattt tatgggatga tgcctgcacc atagccgcat
     4021 tgtgtgtcaa cgactacatt catgccgtct atgattttgt cgaacaggca ggatattgcc
     4081 gcaacggctt ccctgaagca ggcggcgaat gggtgaaagt cgaaaaccgc gtcttggatg
     4141 atgaattgct ggacaaaatc ctatcccgag aacctacata accctcacaa aaggataccc
     4201 aaatgcccct actagacagt ttcaaagtcg atcacacccg tatgcatgcc cccgccgtac
     4261 gcgtggcgaa aaccatgacc acgcccaaag gcgacaccat taccgtgttc gacctgcgct
     4321 tttgcattcc caacaaagaa atcctgcctg aaaaaggcat acacacgctg gagcatttgt
     4381 tcgcaggttt tatgcgcgac cacttgaacg gcaacggcgt ggaaatcatc gacatttccc
     4441 cgatgggctg ccgcaccggt ttctacatga gccttatcgg cacgccttcc gaacagcagg
     4501 tcgccgatgc atggctcgcc tcgatgcagg atgtgggcaa tgtcaaagac caaagcaaaa
     4561 tccccgagtt gaacgaatac caatgcggca cttatcaaat gcactcgctc gccgaagcgc
     4621 agcaaatcgc gcaaaacgtg ttggcgcgca aagtggcggt gaacaaaaac gaagagctga
     4681 cgctggatga agggctgctg aacgcctaat ccgccaaaaa tgccgtctga acaagggttt
     4741 cagacggcat ttgccttttc cgttataatc cggggttgtg cggggtcggg ttttaagccg
     4801 gcatcgtcct tccctatttt tttctgtccc ttatcggttt taagcgggtt ttttatgtcc
     4861 aacagaccta cactcctcct cgttgacggc tcgtcctacc tctaccgtgc gtatcacgcg
     4921 atggggcaaa acctgaccgc ccccgacggc gcgccgacgg gtgcgctgta tggcgtattg
     4981 aatatgttgc gccgtttgcg gtcggaatat ccgcacgatt attgcgcggt ggtttttgat
     5041 gcgaaaggca aaaatttccg ccatcaaatg tttgaagaat acaaggcgac gcgcccgccg
     5101 atgcccgacg atttgcgccc gcaggcggaa gcactgccgg atttagtgcg cctgacaggc
     5161 tggccggtat tggtgattgg gcaggtggag gcggacgatg tgatcggcac gctggcgaaa
     5221 cagggggcgg aacatggttt gcgagtcatt gtttcgaccg gcgataagga catggcgcag
     5281 ttggtggatg agcgcgttac gctggtgaac acgatgagcg gagaaacgct ggatattgaa
     5341 ggcgtgaagg caaaattcgg cgtgcgcccc gaccaaatcc gcgattatct cgcgctgatg
     5401 ggcgacaagg tggacaacgt gccgggcgtg gaaaaatgtg gcccgaaaac ggcagtgaag
     5461 tggctggaag cctacggctc gttgcaaggc gtgatggaac acgcttcgga aatcaagggc
     5521 aaagtgggcg agaatctgcg tgccgccctg ccgcgcctgc cgctgtcgta tgatttggta
     5581 acgattaaaa ccgatgtgga cttgcacgcc gagctttcag acggcatcga aagcctgcgc
     5641 cgtaccgcgc cgaaatgggc gcaactggtt gtcgatttca aacgctgggg cttccgcacc
     5701 tggctgaaag aagcggaatc aaacatgaat accggctcga ccgatgatgt gttcggcagc
     5761 gacagcatcg gtgagcaggc ggctttgaat gcggaaatgc cgtctgaaaa acaagctgaa
     5821 aaagccaccg cccccgaaag gctggattat caagccgtta ccaccgaagc gcagtttgcc
     5881 gctttgttgg acaaactgtc gcgggcgaac acaatcggca tcgatacgga aaccacgtca
     5941 ttagacgcga tgaacgcctc gctggtcggc atcagcatcg ctttccaagc aggcgaagcg
     6001 gtttacatcc ccgtaggaca cagcctgacc gcagcgcctg aacagcttga tttacaagac
     6061 gtattaggcc gtctgaaacc gcatttggga aaccccgccc taaaaaaaat cgggcaaaac
     6121 ctcaaatacg accaacacgt tttcgccaac tacggcatcg ccctgaacgg cattgccggc
     6181 gacgccatgc tcgcttccta catcatcgag agccatctcg gacacggctt ggacgaattg
     6241 tccgaacgct ggctcggctt ggaaaccatt acctacgaat cgctgtgcgg caaaggcgcg
     6301 aagcaaatcg gttttgccga tgtcgccatc gggcaggcga ccgaatacgc cgcccaagac
     6361 gccgatttcg ccctgcgcct cgaagcgcac ctgcgcgcgc aaatggacga aaaacagctt
     6421 gaaatgtatg aaaaaatgga gctgcccgtc gcgcaggtat tgtttgaaat ggaacgcaac
     6481 ggcgtgcaaa tcgaccgcgc cgaactcgcc cgccaaagcg cggaactcgg cgccgagctg
     6541 atgaagctcg aacaacaagc ctatgccgcc gcagaccagc cgttcaacct caattcgccc
     6601 aaacagctgc aagaaatcct gttcgacaaa atgggcatcc ccaccaaagg cctgaaaaaa
     6661 accgccaaag gcggcatttc caccaacgaa gccgtgctcg aacagctcgc gcccgactac
     6721 cccctgccta aaatcatcct gcaaaaccgc agcctggcga agctcaaatc cacctacacc
     6781 gacaaactac ccgaaatgat ttcccccaag gacggccgcg tgcataccac ctacgcccaa
     6841 gccgtcgcca ttaccggccg cctcgccagc aacaacccca acctgcaaaa catccccatc
     6901 cgcaccgccg aaggccgccg cgtgcgccgc gcctttaccg caccgcaagg cagcgtcatc
     6961 gtttccgccg actattccca aatcgagctg cgcattatgg cgcacctctc cggcgacaaa
     7021 accctgattg ccgcgttcca aaacggcgaa gacgtacacc gccgcaccgc cgccgaagtg
     7081 ttcggcactg cgcccgaaaa cgtctcgtcc gagcaacgcc gctacgccaa aaccatcaac
     7141 ttcggcttaa tttacggtat ggggcaatac ggtttggcaa aatcattggg catcgacaac
     7201 ctttccgcca aaaactttat cgaccgctac ttcgcccgct accccggcgt cgccgaatac
     7261 atgcagcgca ccaaagaaca agccgccgcc caaggctacg tcgaaaccct gttcggcaga
     7321 aggctctatt tgcccgacat ccgcaacaaa aacgccaacg cccgcgcagg agccgagcgc
     7381 gccgccatca atgcccccat gcaaggcacc gcgtccgacc tcatcaaacg cgccatgata
     7441 gacgtgcgaa actgtctttc agacggcatc ggcagcaaac tggtcatgca ggtgcatgac
     7501 gaactggtgc tggaagtcgt tgaaaccgaa ctggattttg tcaaagaaaa actgccgcag
     7561 attatggcga aagtggacgg cggattattg gatgtaccgc tggtggctga ggttggcgta
     7621 ggggagaatt gggaagaggc acattgattg aaaggtgtta tatgctatct ttatttaaat
     7681 aaaatttaat ttttggtata ttttttctaa atgttccta
//
LOCUS       DAWKST010000093         7715 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.093, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000093 DAWKST010000000
VERSION     DAWKST010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.093"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(181..1482)
                     /locus_tag="WJL87_001371"
     CDS             complement(181..1482)
                     /locus_tag="WJL87_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ7799793.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCAAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPDLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDDPSDGNPEHTLRLTLWQQGQTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAAAHKTDPDGT
                     FVRRHIPELAHLSADTVHTPWRFACSVDTHGYPAYPVAGIP"
     gene            complement(1472..2383)
                     /locus_tag="WJL87_001372"
     CDS             complement(1472..2383)
                     /locus_tag="WJL87_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ7799794.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAAGALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGSLVRLLPEYTVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGYRNAV"
     gene            2491..2889
                     /locus_tag="WJL87_001373"
     CDS             2491..2889
                     /locus_tag="WJL87_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ7799795.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            2938..3543
                     /locus_tag="WJL87_001374"
     CDS             2938..3543
                     /locus_tag="WJL87_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ7799796.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEIVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALCAVVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            3729..5840
                     /locus_tag="WJL87_001375"
     CDS             3729..5840
                     /locus_tag="WJL87_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ7799797.1"
                     /translation="MKISFHLALLPTLIIASFPVAAADTQDNGEHYTATLPTVSVVGQ
                     SDTSVLKGYINYDEAAVTRNGQLIKETPQTIDTLNIQKNKNYGTNDLSSILEGNAGID
                     AAYDMRGESIFLRGFQADASDIYRDGVRESGQVRRSTANIERVEILKGPSSVLYGRTN
                     GGGVINMVSKYANFKQSRNIGAVYGSWANRSLNMDINEVLNKNVAIRLTGEVGRANSF
                     RSGIDSKNVMVSPSITVKLDNGLKWTGQYTYDNVERTPDRSPTKSVYDRFGLPYRMGF
                     AHRNDFVKDKLQVWRSDLEYAFNDKWRAQWQLAHRTAAQDFDHFYAGSENGNLIKRNY
                     AWQQTDNKTLSSNFTLNGDYTIGRFENHLTVGMDYSREHRNPTLGYSRAFTASIDPYD
                     RASWPASGRLQPILTQNRHKADSYGIFVQNIFSATPDLKFVLGGRYDKYTFNSENKLT
                     GSSRQYSGHSFSPNIGAVWNINPVHTLYASYNKGFAPYGGRGGYLSINTSSSAVFNAD
                     PEYTRQYETGVKSSWLDDRLSTTLSAYQIERFNIRYRPDEQNDPYTWAVGGKHRSRGV
                     ELSAIGQIIPKKLYLRGSLGVMQAKVVEDKENPDRVGIHLNNTSNVTGNLFFRYTPTE
                     NLYGEIGVTGTGKRYGYNSRNKEVTTLPGFARVDAMLGWNHKNVNITFAAANLLNQKY
                     WRSDAMPGAPRTYTARVNYSF"
     gene            6043..6702
                     /locus_tag="WJL87_001376"
     CDS             6043..6702
                     /locus_tag="WJL87_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEZ7799798.1"
                     /translation="MKTAILIDGAFFIKRVRSLGLSQNPHDAKRIADLAFRYSMLHLK
                     DKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFSKTPEAIFRCNLHTELKKKR
                     KLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVVLDVKQKGVDMRIGLDISSI
                     TLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWNHVPPDLMEHIDGLKTKCPK
                     P"
     gene            6873..7568
                     /locus_tag="WJL87_001377"
     CDS             6873..7568
                     /locus_tag="WJL87_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ7799799.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSAQSSSSAPAAPAE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
BASE COUNT         1901 a         1956 c         2066 g         1792 t
ORIGIN      
        1 ttgtccttct tcatcgactt ctatttttgc cgacgatggg aacggtggcg gcttgggtgg
       61 cggtgatttg ggcatacctg atgattgaaa gtgaaaaaaa cggaagatat tgagtcattc
      121 ggacgcaatg ccgtctgaaa cggaagttca gacggcattt gttttaggtt gccgtaccgc
      181 ttagggaata ccggcgacag gataggcggg atagccgtgg gtatcgaccg aacaggcaaa
      241 ccgccaaggc gtgtggacgg tgtcggcgga caggtgggcg agttcgggaa tgtgccgtct
      301 gacaaaggtg ccgtcggggt cggtcttgtg tgcggcggcg gcaatgtcgg ggcaggtgtg
      361 ccgtgaggcg gcaagccgcc agttgccttg gttgattgct gcatcgaaat cggtcagctg
      421 tcgggcaaac catatctcgc cttcgcggcg ggggaggttt aaaacgtggc agaaaaaatc
      481 cgcgctcaag cgtctcaggg cggggtggag gctgccggtt ttgtgcaaac agcgcatcgc
      541 ggcatcgata atcggaatgc cggtctggcc ctgctgccaa agcgtcaggc gcagggtgtg
      601 ttcaggattg ccgtctgaag ggtcgtcatc cgtgtgctgc aaggcaagtt gaaggaaaaa
      661 atcgcggcgg atgatgttgt ccgcccacgc gttcagacgg cgttcgaggc tttcccgcgc
      721 gagcaggcgc ggcgagatgc agccggcact caaatacgcg cccatcagcg aagtgtgttt
      781 gcgcgagggg aaatccttta aaacggagta ggaatccgcc tgttcgagaa accgccgcca
      841 ctgccgccaa gccgccgttt cgccgctgtt ttgcggcagg aagatgccgt ctgaaagggc
      901 ggcaggcggc ggggcggaaa ggttttcggg gaagggttgg cggtatgccg cgaataggtc
      961 cggaccggcg gggggctgct tggaaaagcg gtcgagccat acttcgcggt agcggtcgaa
     1021 atcggtgtgt gccgtgccgc cgtcgggtat caggtcggtt ttgccgaaaa cggcacggtc
     1081 gttgacgaag gttaacgcga tgccgtgttt gtccaattca tgccaaaggg cgttgtcggc
     1141 gagtttgtcg gcaaaagtat gggattcgtc ggcgatgacg gcgcggatat tgaggcggac
     1201 ggcgagccgg acgagctcgg caggcgatgc cgccgtgtag agcgggatgc cgcgccctgc
     1261 aagcccttgg gcgagttcgg cggcggattg gcggtagaac gcggcgcggc gagggttgtc
     1321 tgtttcggca tcgtcaatcc aaataccgat aacgggcaaa ccttcggcaa cggcggcgca
     1381 taaggcggcg ttgtcgcgga tgcggaggtt ttggcggaac cagacgagcg tgtgtgcggc
     1441 gcacgtgtcc gcataaaggg ggcgggcggt ttcagacggc atttcggtag cctttcctgc
     1501 tggcgatttt ttcgttcaga aaatcgatga agctgcggac tttcgcgctt aagaacgccc
     1561 tgtccgcata aacggcattc aggcggtcgg tcgggacggt atattcgggc agcagcctca
     1621 ccagcgagcc gcagcgcaaa tcgtgttccg ccgcccaaag cggctgataa ccgatgcacg
     1681 cgcccgcctt aatcatttcg cgcatcatca gcgtgttgtc ggtacggatg acgggggtca
     1741 gttcaagccg gtattttttg ccgtccgatt tgcgggtgag gtcgagtttc tgctggttgg
     1801 tgtaggtcgg caggacggcg ggcagccccg ccacttcttc cggcgtttcc ggcacgccgt
     1861 tgcgcctcag gaaatcgggc gaggcgagca gggcaaattc gatttccgcc agtgggcgcg
     1921 caatcagcga cggggacagg gtttgggaaa cgcgcaacgc caaatccacg ccttcggcaa
     1981 tcaaatcgac gtggcggttg tccaaaatca gttctaatgt cacttcggga taacgttcgc
     2041 ggtattccgc cagccagttg catatctggc tgccggcaaa ccacagtggc atcgttacgc
     2101 gcagcagccc ctgcggtttt tccgtccccc cggcggcttt ttgcgcggca tcgtcgagcg
     2161 tgtcgagcgc gtaactgcat tgccggtagt attcttcccc ggcttcggtc aggctgaggt
     2221 tgcggctgtt gcggtgcagg agtttggctt ggacggtgtt ttccaagtgg ctgacgtgtt
     2281 tgcttgccat tgcggtggag atgccgagcg cgccggcggc gcgggtgaag ccgccgcttt
     2341 ggacgacttg gcggaaaacc ttgaggctga acagggtgtc catattttct tgtgtggaaa
     2401 agttgtatca ataaaagcag tatatatttg aaaaggggaa acatctatac tctaccgcct
     2461 gaaatgaaga caaatatcaa aggagctttt atgtccgatt gctgcaaccg tatccaaccg
     2521 gttttgcttt ctgttttgcg tatcgtaacc gcttacctgt ttttgttgca cggtacgtcg
     2581 aaaatcttcg ccttccccat tgaaatgggc agcggttcgc ccggcgggct gttgctgctt
     2641 gccggtattt tagaaattgt cggcggcatt ttgctggtgt tgggcctgtt tgcgcgccct
     2701 gccgcgtttg ttttgtccgg ccagatggcg gttgcctatt ttatggcgca cgcttccgga
     2761 aatgctttgt tcccgattgc caacggcggc gagtccgcag tgctgttctg cttcgtattc
     2821 ctctatatcg cggcggcggg cggcggagca tggtcgctgg acaggctgtt tttcaagcgt
     2881 aaagcctgaa tcagactgcc taaagtgtat tttgttgaat gtttttgagg aaaagaaatg
     2941 acccgtcaat ctctgcaaca ggctgccgaa agccgccgtt ccatttattc gttaaataaa
     3001 aatctgcccg tcggcaaaga tgaaatcgtc caaatcgtcg aacacgccgt tttgcacaca
     3061 ccttcttcgt tcaattccca atctgcccgt gtggtcgtgc tgtttggcga agagcatgat
     3121 aaggtgtggc aatttgtcga agacgcgctg tgtgccgtcg tgcctgccga cagttttgaa
     3181 ccgaccgcgc aaaaattgaa cctgtttaag gcgggtgcgg caactatttt gttttatgaa
     3241 gatcaaaatg tcgtcaaagg tttgcaggag cagttccctg cttatgccgc caactttccc
     3301 gtttgggcgg accaggcgaa cgcgatggtg cagtatgccg tttggacgac acttgccgcg
     3361 gtcggcgtag gtgcaaacct gcaacattac aatcccttgc ccgatgcggc gattgccaaa
     3421 gcgtggaata tccccgaaaa ctggttgttg cgcgcacaaa tggttatcgg cggtattgaa
     3481 ggggcggcag gtgaaaagac ctttgaacca gttgcagaac gtttgaaagt gttcggcgca
     3541 taatttcgcg gtcaaaaaaa tgccgtctga accctgttca gacggcattt ttcagtatca
     3601 ggcggcgagt tttccgcatt ctgagacctt tgtttacaaa tatcatgttc aatatagtta
     3661 aaagaaatta ttctcatttc ctccgtgagg caatataatt cggttgtttt gttaaattga
     3721 gtataaaaat gaaaatatca tttcatttag ctttattacc cacgctgatt attgcttcct
     3781 tccctgttgc tgccgccgat acgcaggaca atggtgaaca ttacaccgcc actctgccca
     3841 ccgtttccgt ggtcggacag tccgacacca gcgtactcaa aggctacatc aactacgacg
     3901 aagccgccgt tacccgcaac ggacagctca tcaaagaaac gccgcaaacc atcgatacgc
     3961 tcaatatcca gaaaaacaaa aattacggta cgaacgattt gagttccatc ctcgaaggca
     4021 atgccggcat cgacgctgcc tacgatatgc gcggcgaaag cattttcctg cgcggttttc
     4081 aagccgacgc atccgatatt taccgcgacg gcgtgcgcga aagcggacaa gtgcgccgca
     4141 gtactgccaa catcgagcgc gtggaaatcc tgaaaggccc gtcttccgtg ctttacggcc
     4201 gcaccaacgg cggcggcgtc atcaacatgg tcagcaaata cgccaacttc aaacaaagcc
     4261 gcaacatcgg tgcggtttac ggttcgtggg caaaccgcag cctgaatatg gacattaacg
     4321 aagtgttgaa caaaaacgtc gccatccgtc tcaccggcga agtcgggcgc gccaattcgt
     4381 tccgcagcgg catagacagc aaaaatgtca tggtttcgcc cagcattacc gtcaaactcg
     4441 acaacggctt gaagtggacg gggcaataca cctacgacaa tgtggagcgc acgcccgacc
     4501 gcagtccgac caagtccgtg tacgaccgct tcggactgcc ttaccgcatg gggttcgccc
     4561 accggaacga ttttgtcaaa gacaagctgc aagtttggcg ctccgacctt gaatacgcct
     4621 tcaacgacaa atggcgtgcc caatggcagc tcgcccaccg cacggcggcg caggattttg
     4681 atcatttcta tgcaggcagc gaaaatggca acttaatcaa acgtaactac gcctggcagc
     4741 agaccgacaa caaaaccctg tcgtccaatt tcacgctaaa cggcgactac accatcggcc
     4801 gttttgaaaa ccacttgacc gtaggcatgg attacagccg cgaacaccgc aacccgacct
     4861 taggttacag ccgcgccttt actgcttcca tcgatccata cgaccgagca agctggccgg
     4921 cttcgggcag attgcagcct atcctcaccc aaaaccgcca caaagccgac tcctacggca
     4981 tctttgtgca aaacatcttc tccgccacgc ccgatttgaa attcgtcctc ggcggtcgtt
     5041 acgacaaata cacctttaat tccgaaaaca aactcaccgg cagcagccgc caatacagcg
     5101 gacactcgtt cagccccaac atcggcgcag tgtggaacat caatcccgtc cacacacttt
     5161 acgcctcgta taataaaggc ttcgcgcctt atggcggacg cggcggctat ttgagcatca
     5221 acacgtcgtc ttccgccgtg ttcaacgccg accccgagta cacccgccaa tacgaaaccg
     5281 gtgtgaaaag cagttggctg gacgaccgcc tcagcactac gttgtctgcc taccaaatcg
     5341 aacgcttcaa tatccgctac cgccccgacg agcaaaatga tccctacact tgggcagtcg
     5401 gcggcaaaca ccgttcgcgc ggcgtggaat tgtccgccat cgggcaaatc atccccaaaa
     5461 aactctatct gcgcggttcg ttgggcgtga tgcaggcgaa agtcgttgaa gacaaagaaa
     5521 atcccgaccg agtgggcatc catttgaata acaccagcaa cgttaccggc aacctgtttt
     5581 tccgttatac cccgaccgaa aacctctacg gcgaaatcgg cgtaaccggt acaggcaaac
     5641 gctacggtta caactcaaga aataaagaag tgactacgct tccaggcttt gcccgagttg
     5701 atgccatgct tggctggaac cataaaaatg ttaacattac ctttgccgca gccaatctgc
     5761 tcaatcaaaa atattggcgt tcggatgcca tgcccggcgc gccgcgcact tatacggcgc
     5821 gggttaatta cagtttctaa tcccgttcgt gcaacagaat gccgtctgaa aatcctgata
     5881 atccgtaaat tcagcacacg aactttttga caggctttat ctacatcagt ataatccaaa
     5941 aagaaatagc gcccttggca gtacgcatta cccattaaat catagcgggt atgccgatcc
     6001 caagggcttt tttatatctt tgacaaatat aggggaagca atatgaaaac cgccattttg
     6061 attgacggtg cattttttat taagcgggta cggtcattag gcctgtctca aaatccacac
     6121 gatgcaaaaa gaattgctga tttggctttc cgatattcga tgctgcatct gaaagataaa
     6181 atcaacggca cagatcaaat ggatgattta tatagaattt tcttttacga ctgcgtgccg
     6241 tttgaaaaga aaatgcacaa tcccgtatct catcgtgcca tagatttttc aaagactccg
     6301 gaagccatat ttcgttgcaa tctgcatacc gaattgaaga agaagcgtaa attagcgtta
     6361 cgtttaggca agctgtcgga caatacagca tggatattaa aaccccaagt catgaaaaat
     6421 cttctgaaaa acccgtcaac tcaaattacg gaaaacgatg tcgtgctcga tgttaaacaa
     6481 aaaggtgtag atatgcgtat aggcttggat atttcatcta ttaccttaaa aaaacaagcc
     6541 gataaaatca tcttgttttc tggtgattcc gattttgtcc cagcagccaa attagccaga
     6601 cgggaaggta tcgattttat tcttgatcct atgtggaatc atgtcccacc tgacttaatg
     6661 gaacacatcg acggattgaa gacaaaatgt ccgaaaccgt aaattactag aacaaaggcc
     6721 gtctgaaaat aaggctaatt atctaagcca tatttcagac ggcctttata aaggctgaaa
     6781 taaaactaaa cacatttttt cactcaaatc gaacacgcct tcaataaaat gccataaaat
     6841 ccgcacatta atctgacaca caagagatac ctatgaaact gaaaacctta gctttgactt
     6901 cattgaccct gttggcattg gccgcttgta gcaaacaggc tgaaaccagt gttccggcag
     6961 acagcgccca aagcagctca tctgctccgg cagcccctgc tgagttgaac gaaggtgtga
     7021 actacactgt attgtctacg cctattccgc aacagcaggc cggtaaaatc gaagtattgg
     7081 aatttttcgg ctacttctgc ccgcattgcg cccatcttga gccggtcttg agcgagcaca
     7141 tcaaaacgtt taaagacgat acctatatgc gccgggagca tgtcgtgtgg ggtgatgaaa
     7201 tgaaaccttt ggcacgtttg gcggccgcag tggaaatggc cggtgaatca gataaagcca
     7261 acagccatat tttcgatgcg atggttaatc aaaaaatcaa tctggccgat accgataccc
     7321 tgaaaaaatg gctgtccgag caaacagcgt ttgacggcaa aaaagtattg gctgcatttg
     7381 aggctcctga aagccaagcg cgtgcggctc aaatggaaga gttgaccaat aaattccaaa
     7441 tcagcggcac accgactgtg attgtcggcg gcaaatacca agttgaattt aaagactggc
     7501 agtctggtat gaccacgatt gaccagttgg tggataaagt acgcgaagag cagaaaaagc
     7561 cgcaataagt tgaggattga atgagtaaag gccatctgaa aataggattt cagacggcct
     7621 tttgtattta ggctttatag aagagatgat tgcttaaagc cttatggttt taaatcagaa
     7681 tatatagcgg attaacaaaa accagtacgg cgttg
//
LOCUS       DAWKST010000094         7691 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.094, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000094 DAWKST010000000
VERSION     DAWKST010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7691
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.094"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(45..461)
                     /gene="dksA"
                     /locus_tag="WJL87_001378"
     CDS             complement(45..461)
                     /gene="dksA"
                     /locus_tag="WJL87_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ7799800.1"
                     /translation="MAKLTEQDILNWSGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(608..1399)
                     /locus_tag="WJL87_001379"
     CDS             complement(608..1399)
                     /locus_tag="WJL87_001379"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ7799801.1"
                     /translation="MNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRERLQKEL
                     GVETSETLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLPVDTLSRYLGG
                     TRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDEAQMHSI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1448..1855)
                     /locus_tag="WJL87_001380"
     CDS             complement(1448..1855)
                     /locus_tag="WJL87_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEZ7799802.1"
                     /translation="MEIRAIKYTATAALLAFTVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRAKEYESAQQSYFYRKIGKFEACGLDWRTR
                     DGKPLIETFKQEGFDCLKKQGLRRNGLSERVRW"
     gene            complement(1855..2541)
                     /locus_tag="WJL87_001381"
     CDS             complement(1855..2541)
                     /locus_tag="WJL87_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ7799803.1"
                     /translation="MTVLQERYREVSDRIGKLVLQAGRKPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAAKHGVAPEEAVALAVEVAKLPNLVVRG
                     LMCVAKANSSETELKTQFQTMQKLLADLNTAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2661..3704
                     /locus_tag="WJL87_001382"
     CDS             2661..3704
                     /locus_tag="WJL87_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ7799804.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3867..5093
                     /locus_tag="WJL87_001383"
     CDS             3867..5093
                     /locus_tag="WJL87_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ7799805.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5379..7529
                     /gene="yccS"
                     /locus_tag="WJL87_001384"
     CDS             5379..7529
                     /gene="yccS"
                     /locus_tag="WJL87_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ7799806.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIITTVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQENVANAYEALGSYLEAKADFFDPDEAEWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNAAQALRAGKDYTYSKRLGRAIEGCRQSLRLLSDGNDNPDIRHLGRLLDNLGS
                     VDQQFRQLQHNGLQVENDRMGDTRIAAIETGSLKNTWQTIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVVVSTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPDMEPDRFDAELDSLRSELDNLRVQSVGAQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1893 a         2538 c         1698 g         1562 t
ORIGIN      
        1 ctgtctgaaa cttagggctt cagacggcat tttgtttgcc gccatcagtc ggcaaactgt
       61 tttttcatcc tctctcggcg ttcttgggac tcaacagata aagtggctgt cgggcgtgcc
      121 agcagccgct tcagaccgat aggctctccc gtatcggcac agaatccata atccccttca
      181 tcaatattgc ggatggtcgc ctgtatttta ctgagaagtt ttcgttcccg atcgcgggta
      241 cggagttcca atgcgtactc ttcttcctgt gtggcacggt cggcaggatc gggggctgat
      301 tcgtgttctt ggagatgccc tgtcgtagcg gaagcatttt cgatgagttc gtcttgcatt
      361 tttaccagca attcgcggaa aaaagccaaa tggtcgtcat tcatataatc gtcttccggc
      421 ccgctccaat tcaaaatatc ttgttctgtc agctttgcca taatgtatat cctctcggtt
      481 caccgaaaaa aacaaagcac cacaaagtgg ctttattcct ttgattttta taaggcttat
      541 tgcctaaggc gtttttgaaa acgcgcatca taacacgttt tggattggtt tttttatttt
      601 ctttacatta ttgatattgc cgttccattt cctgcgaacg gcgcacacag gcacaaacgc
      661 cctcacttat ggcttcggcg acacgatgcc gcctgaaggc ttccacggct tcgtgggttg
      721 tcccgccttt tgacgttaca tttttttgaa gcttctcgaa atcttcaccc gtctgctcgg
      781 caagggcaac cgctccttta aacgttgcca gactgagcgc gcgtgcttct gccatatcaa
      841 acccttgtcg gatggcggca ttttgcaatg cgtccagcag ataaaacaca tatgccggcc
      901 cgctgccgct gataccggta atgctgtgca tttgcgcttc atcttccaac caaacagtca
      961 aaccgactga tttcatgatt cgatcggcaa tcctgcggtc tgtttccgat acttccgctt
     1021 cggcatacat accggatacg cccagcccga tttttccggg tgtattcggc ataacccgga
     1081 caatgcggcg tgttcccccg aggtaacggc tgagcgtatc gaccggcaat ccggctgcga
     1141 cagaaagcac caatgcgccg ttggtgcgga tatttttgca cgcggcttcc atatcctgcg
     1201 gcttgacggc aaggattaaa acatcgtcgg aatgaagctc cggcaaggtt tcagaagttt
     1261 cgacccccaa ctctttttgc aaacgttcgc gcttttccgc accccggttg gcaatatgga
     1321 tgcggtaacc gccttgtttg accaatccgc ccgcaacggc agccgccata ttgccgccgc
     1381 cgagaaaata aacattcatt atttctaccc tattcaaaat tacctgacta aattcattcc
     1441 caatttttta ccatcggacg cgctcggaca gaccgttgcg ccgcaacccc tgctttttca
     1501 aacaatcaaa accttcctgt ttgaacgtct caatcaaagg tttgccgtca cgcgtacgcc
     1561 aatccaaccc gcaggcttca aacttcccta ttttcctgta aaagtaagat tgttgtgcgg
     1621 attcatattc ctttgccctg acggaacgat tgccgtctga aagcggtatc tcgtagtcct
     1681 ctaaagacgg cggactctcg ccgccaatat cccaaaaatc gatggcggcc ggttttctcg
     1741 gcttacacca gccggacagg gacgaacact cataccaacc cgccaaccgg cagcctgcaa
     1801 ccgtaaaggc aagcaacgca gccgttgccg tatattttat tgcccgaatt tccatcatcc
     1861 ccttttcccg aaaatcgcgc tgccgatacg gacgtgtgtc gcaccgcact caatggcggc
     1921 aggcatatcg tccgacatcc ccatagacag cacgtccgcc ttaacgccag ccgtattgag
     1981 gtcggcaagc aacttttgca tggtctgaaa ctgggttttc aactccgttt cactgctgtt
     2041 ggctttggca acgcacatca gtccgcgcac gacaaggttg ggcaacttcg ccacttcgac
     2101 cgcaagcgcg accgcttctt cgggagcaac accgtgcttt gccgcctcgc ctgcaatatt
     2161 cacctcgata cacacctgca aaggcggcat tgaagaagga cgctgcccac tcagccggac
     2221 ggcggtcttt agacggcata cggtatgcac ccaatgcgcg cgttcggcga caaacttggt
     2281 tttgttggac tgcacatcgc cgatgacgtg ccacacgatg tcggtcaaat ccgccaactc
     2341 ttccgttttg ccgtaccact cctgaatata gttctcgccg aaatcacgct gtccggcggc
     2401 gtaaacttcg cggatgccgt ctgaagggaa agtcttaccg acggcaatca ggctgacgga
     2461 atgcggcttc cttcccgcct gcagaaccaa ttttccaata cggtcggaca cctcccgata
     2521 acgttcttgc aacaccgtca tagattatcc cctaattaaa aatgattaaa cagttgaaac
     2581 ccctccagtc agggcggtac aatcaaggtt gttagaacca ttccaaccaa tcgaaacatt
     2641 atactaaaca gagccgcatt atgcagatta ccgacttact cgccttcggc gctaaaaaca
     2701 aagcatccga ccttcacctg agttcgggca tatcccctat gattcgggtt cacggcgata
     2761 tgcggcgcat caaccttccc gaaatgagcg cggaagaggt cggtaatatg gtaacttcgg
     2821 tgatgaacga ccaccagcgg aaaatctacc agcaaaactt ggaagtcgac ttctcgttcg
     2881 aactgcccaa cgtcgcccga ttccgcgtca acgccttcaa caccggccgc ggtcccgccg
     2941 ccgtattccg caccattccc agcaccgtct tatcgctgga agaattgaaa gccccgagca
     3001 ttttccaaaa aatcgcagaa tcgccgcgcg gcatggtatt ggttaccggc cctaccggtt
     3061 cgggcaaatc gaccacgctt gccgcgatga tcaactacat caacgaaacc cagccggcac
     3121 acatcctgac catcgaagac ccgatcgaat tcgtccacca aagcaaaaaa tccctgatta
     3181 accagcgcga gctgcaccag cacaccctca gcttcgccaa cgcgctgcgt tccgcattgc
     3241 gcgaagaccc cgacgttatc cttgtcggcg agatgcgcga ccccgaaacc atcggcttgg
     3301 cactgaccgc cgccgaaacc ggacacttgg ttttcggcac gctgcacacg accggcgcag
     3361 caaaaaccgt cgaccgtatc gtggacgtat tcccggcggg agaaaaagaa atggtgcgct
     3421 ctatgctgtc cgaatcgctg accgccgtca tctcccaaaa cctgctgaaa acgcacgacg
     3481 gcaacggccg tgtcgcctcg cacgaaatcc tgattgccaa ccccgccgtc cgcaacctca
     3541 tccgcgaaaa caaaatcacg cagattaact ccgtcctgca aaccgggcag gcgagcggta
     3601 tgcagacaat ggaccaatcg ctgcaatcgc tggtgcgcca agggctgatc gcaccggaag
     3661 tcgcacgcag acgcgcgcaa aacagcgaaa gtatgagttt ctgacacaca accgccttcc
     3721 ggccataccg gcgggaaaac aaggcgcaaa cacgcggggc gggacgcagc atcccgcccg
     3781 gctacctttc cgaacaagaa gcgtccgcct tcctgttgaa acctgccgcc gcaaactgca
     3841 aggcttaaac cgaaaagaag ctaacgatga ataccgataa cctgcacgac atcttggacg
     3901 aaatggttca agtgtattct caaaaaaaac aaagccgatc cgaaaccccg gccgaaatcg
     3961 gcgcacactt ccacccgctg ctcgaccgcc tgtgcgaaac cgcagaagca caaaacgcgt
     4021 ccgacatcct tatcagcaaa ggattcccgc cctcgttgaa aatcaacagc gcattaaccc
     4081 cgcagccgca aaaggcgctg acaggcgagg aaaccgccgc catcgccgca tcgacgatga
     4141 acgccgaaca atcggaaata ttccggcgcg acggcgaaat caactactcc gtccagtcgc
     4201 gcagcggcac gcgctaccgc gccaacgcct accacagcca aggcagcgca ggtttggttt
     4261 tgcggcgcat caaccacgtc atcccgcaaa tgcaggaatt gggcctgccc gaaaaactca
     4321 aagacctcgc cgtcgcaccg cgcgggctgc tgattatcgt cgggcctacc ggttcgggca
     4381 aatccaccac gatggcgact atgctcgaac accgcaacaa aaccctgccc agccatatcg
     4441 ttaccatcga agacccgatt gagtttatct acaaaccgcg ccgctgcatc tttacccagc
     4501 gcgaaatcgg cgtcgacacc ataaactggc agacggcggt acaaaacgct atgcgccaat
     4561 cccccgacgt ggtctgcatc ggcgaagtcc gcagcaggga aagtatggaa tacgcgatgc
     4621 agctcgccca aaccggccac ctgtgcattt ttacgctcca cgccaacacc gcgccgcagt
     4681 cgctcgaacg catactcaac ttctacccca aagaacagca caaccaaata ctgatcgaca
     4741 tcgccctcaa cctgaccggc atcatctgcc aacgcctcgc cctcaaacaa gacaaaacgg
     4801 gcaggacggc ggttgtcgac ttgctcatca acacgcccgc catccaagac ttcatcctga
     4861 agggcgacct gatgaacatc agtaaaatca tggaaaccgc caaaaccgac ggaatgcaga
     4921 cgatggatca aaaccttttc gaactgtacc gtcacggcat catcagttac gaagaagccc
     4981 tgcgccagtc cgtttccgcc aacaacctgc gattgcacat ccaactgcac aaagaaggca
     5041 aaacgcccga actcctttac gacagggtca acggtctcaa cctcatttcc tgatccgcaa
     5101 aacccaatgc cgtctgaaaa ccgcatcccc gttttcagac ggcatgattt tatccgcccc
     5161 attcatgtgc tacactttat agtggattaa atttatagtg gattaacaaa aatcaggaca
     5221 aggcgacgaa gccgcagaca gcacaaatag tacggaaccg attcacttgg tgcttcagca
     5281 ccttagagaa tcgttctctt tcagctaagg cgaggcaacg ccgtactggt ttttgttaat
     5341 ccactatatt cacttcataa caataaaccg gtaaaaccat gaaaacccca ctcctcaagc
     5401 ctctgctcat tacctcgctt cccgttttcg ccagtgtttt taccgccgcc tccatcgtct
     5461 ggcagctagg cgaacccaag ctcgccatgc ccttcgtact cggcatcatc gccggcggcc
     5521 ttgtcgattt ggacaaccgc ctgaccggac ggctgaaaaa catcatcacc accgtcgccc
     5581 tgttcaccct ctcctcgctc acggcacaaa gcaccctcgg cacagggctg cccttcatcc
     5641 tcgccatgac cctgatgacc ttcggcttta ccattttagg cgcggtcggg ctgaaatacc
     5701 gcaccttcgc cttcggcgca ctcgccgtcg ccacttacac cacacttacc tacacccccg
     5761 aaacctactg gctgaccaac cccttcatga ttttatgcgg caccgtactg tacagcaccg
     5821 ccatcatcct tttccaaatc atcctgcccc accgccccgt tcaagaaaac gtcgccaacg
     5881 cctacgaagc gctcggcagc tacctcgaag ctaaagccga ctttttcgat cccgacgaag
     5941 ccgaatggat aggcaaccgc cacatcgacc tcgccatgag caacaccggc gtcatcaccg
     6001 ccttcaacca atgccgttcc gccctgtttt accgccttcg cggcaaacac cgccacccgc
     6061 gcaccgccaa aatgctgcgt tactactttg ccgcccaaga catacacgaa cgcatcagct
     6121 ccgcccacgt cgattatcag gaaatgtccg aaaaattcaa aaacaccgac atcatcttcc
     6181 gcatccaccg cctgctcgaa atgcaggggc aggcatgccg caatgccgcc caagcactca
     6241 gggcgggcaa agactacaca tacagcaaac gcctcggccg cgccatcgaa ggctgccgcc
     6301 agtcgctgcg cctcctttca gacggcaacg acaatcccga catccgccac ctcggccgcc
     6361 tgctcgacaa cctcggcagc gtcgaccaac aattccgcca actccagcac aacggcctgc
     6421 aggtagaaaa cgaccgcatg ggcgacaccc gcatcgccgc catagagacc ggcagcctca
     6481 aaaacacctg gcagacaatc cgtccgcagc tgaacctcga atcaggcgta ttccgccatg
     6541 ccgtccgcct gtccctcgtc gttgccgccg cctgcaccat agtcgaagcc ctcaacctca
     6601 acctcggcta ctggatactg ctgaccgccc ttttcgtctg ccagcccaac tacaccgcca
     6661 ccaaaagccg cgtccgccag cgcatcgccg gtaccgtact cggcgtaatc gtcggctcgc
     6721 tcgtccccta cttcaccccg tctgtcgaaa ccaagctttg gattgtcgtc gtcagcacca
     6781 ccctcttttt catgacccgc acctacaaat acagcttctc gacatttttc atcaccattc
     6841 aggcactgac cagcctgtcg cttgcagggt tggacgtata cgccgccatg cccgtgcgca
     6901 tcatcgacac cattatcggc gcatcccttg cctgggcggc ggtcagctac ctatggccag
     6961 actggaaata cctcacgctc gaacgcaccg ccgcccttgc cgtatgcagc aacggcgcct
     7021 atctcgaaaa aatcaccgaa cgcctcaaaa gcggcgaaac cggcgacgac gtcgaatacc
     7081 gcgccacccg ccgccgcgcc cacgaacaca ccgccgccct cagcagcacc ctttccgaca
     7141 tgagcagcga acccgcaaaa ttcgccgaca gcctgcaacc cggctttacc ctgctcaaaa
     7201 ccggctacgc cctgaccggc tacatctccg ccctcggcgc ataccgcagc gaaatgcacg
     7261 aagaatgcag ccccgacttt accgcacagt tccacctcgc cgccgaacac accgcccaca
     7321 tcttccaaca cctgcccgac atggaacccg accgcttcga cgcagaatta gacagcctgc
     7381 gcagcgaact tgacaacctg cgcgtgcaaa gcgtcggcgc gcaaagccat atcctcctcc
     7441 aacagctcca actcatcgcc cgacagctag aaccctacta ccgcgcctac cgccaaattc
     7501 cgcacaggca gccccaaaac gcagcctgaa aaagtttcgg cattttgtaa gagagagcag
     7561 attgtcagac aggttgcaag atagtggata aagctttgcg cagggtaaat gcgtagcaac
     7621 tgaaccgtca ttcccacgaa cctacatccc gtcattccca cgaaaacaga aaaccaaaaa
     7681 cagaaaccta a
//
LOCUS       DAWKST010000095         7669 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.095, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000095 DAWKST010000000
VERSION     DAWKST010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7669
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.095"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(308..730)
                     /locus_tag="WJL87_001385"
     CDS             complement(308..730)
                     /locus_tag="WJL87_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ7799807.1"
                     /translation="MQVTSKWIDGMCFVGTTESGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(848..1519)
                     /locus_tag="WJL87_001386"
     CDS             complement(848..1519)
                     /locus_tag="WJL87_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEZ7799808.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QSAKP"
     gene            complement(1535..2161)
                     /locus_tag="WJL87_001387"
     CDS             complement(1535..2161)
                     /locus_tag="WJL87_001387"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ7799809.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AERGIPTDSLHHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGVVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2268..3074)
                     /locus_tag="WJL87_001388"
     CDS             complement(2268..3074)
                     /locus_tag="WJL87_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7799810.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAASEPAAASAAQGDTSSIG
                     STMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQEVMM
                     KFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGEGKQP
                     SKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEATFYI
                     PSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3333..3773)
                     /locus_tag="WJL87_001389"
     CDS             complement(3333..3773)
                     /locus_tag="WJL87_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ7799811.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGRLQEL
                     DKMLWQYEAESFIPHEIWEMEEAMPSETSVLLACGGNPPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3836..5242)
                     /locus_tag="WJL87_001390"
     CDS             complement(3836..5242)
                     /locus_tag="WJL87_001390"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ7799812.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDIHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHSAHEAVVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLVDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5412..6527
                     /gene="lptF"
                     /locus_tag="WJL87_001391"
     CDS             5412..6527
                     /gene="lptF"
                     /locus_tag="WJL87_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ7799813.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGEFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6524..7594
                     /gene="lptG"
                     /locus_tag="WJL87_001392"
     CDS             6524..7594
                     /gene="lptG"
                     /locus_tag="WJL87_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ7799814.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISIGNTGIWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLTGRFFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
BASE COUNT         1662 a         2096 c         2021 g         1890 t
ORIGIN      
        1 aataaattaa tatatggctt aaaataacgg gatgcccgcc ttccgcccgc ccgcagaagc
       61 aggcgggtat cattttaaaa cgcggcattt aaaatttgac cgaaaattgt tgacaatccg
      121 gaatcaagtc tgcacaatac cccgacaagt ccaagtatta taaaggctga ataaagagga
      181 aacagcaggc agatatattc gggaggtgca gtccgaatat atctgctttt ttatgcgcct
      241 ccggattgcc tgccgcacct ttcccttcag acggcatcag ccgtttcccc ataatgccgc
      301 ccgatgccta tttatctgcc ccggcaattt caaaactgtg ggtaatcttt gccgctttgc
      361 ccaacataat cgaagccgaa cagtattttt cggcagacat ctgaacggcg cgctcaatgg
      421 ccgattcttt caaatcatgc ccgaatactt tgaaatggat gtggatttcg gtaaacacgc
      481 gcggcgcatc gtccgcccgt ttcgccgtaa ccgtcgcacg gcagtcagtc actttctgac
      541 gctgtttttc ggcaatcatc accacatcga tgctcgaaca gcccgccacg cccaacagca
      601 gcatttccaa agggctgggc ccgcgcttgg ccgcaccttc tgccgccgaa ccctccataa
      661 cgacgctgtg cccgctttcc gtcgtgccga caaagcacat tccgtctatc cattttgatg
      721 taacctgcat ggtgtcattc ctgaaaatag cgttaaaacc gctttgcata tggcgttatt
      781 gtaaacaatt tcaagcggct tatgcagaaa tatggacaaa acggcaaaaa aacgcctgaa
      841 aaccgattta cggtttggcg gactggccat tgatctgcac cgatttgagt ttcagcgtat
      901 aggttttgcc gtcgtcggta tagccgattt gtgccggaat attgttcagg gacggtgcga
      961 agaaatacat taccgtatcg tcgccgcgcc gcacccgata tttgacgact tcggtttcca
     1021 cgccgcctat gctgtatttt cctgtacccg ccttattcaa accgccgacg gaataaagtt
     1081 ttttgccgtt ggtgattttc agccccgggg ggagtttcgc gtcatttgcc gccaactgcc
     1141 aggcaagcgt gaacagatcc atagccttgg ggctttgctc ggttttgctc tcgcccgctt
     1201 tgccgtaggt tacgctgccg tcggcgaatt tggcttccgc atacagtttg cccctgcgta
     1261 tgtctctata gtaggtaggg tgcagggtat tgccgacaac cgtaccgccg gactcgaaac
     1321 ggatattgta tagcggcact ttaatcgtcg aaacgatttt gtaagcattg ccgctgcgtt
     1381 caaatgtcat cgtggcggga atgccgtagc tgccggaata gtgcagcacg gcggattggg
     1441 gcagccctgc cgcatacgcg cacggcaggg cggcggacaa aatggcggcg gaaaatatat
     1501 ttttaaaagt cttcatcatt tgctcccgcc cggtttacgc cgtcagaaaa cgggcggcat
     1561 cggcgttttc cgaatttctg acgcggtttc cctcgataat caggcggccg gcggcaaaat
     1621 cggcaacggc tttcggatag agtttatgct cgacagccaa aacccgggcg gcaacatcgt
     1681 ctgccgtatc gccgtcgagt atcggcacaa ccccttgcga tacaatcggg ccgcaatcca
     1741 gttcggcagt aacgaaatgg atggtgcagc cggcaacgcg acaacctgcc tccaaagcgc
     1801 gttcgtgcgt atgaagtccg gtaaacgagg gaaggatgga cgggtgaatg ttcatcagcc
     1861 tgccttcgta atgggcacag aactcagggg tcagaatccg cataaaacct gccaaaacca
     1921 ccaagtcggg ttgatatgcg tcgatttttt ccatcatggc ggtatcgaag gcaagtcgtg
     1981 attcaaagtt tttatgatgc aggctgtcgg tcgggatgcc gcgttcggcc gcccattgca
     2041 aaccggcagc cgtttcgctg ttgctcaaca cggcggcaat gcggacgttg ggaatggcgg
     2101 cattgacgat tgcctgcata ttgctgccgc gtccggaaat caggatgacg atgtttttca
     2161 taatggtacg cttttgaaag ggatgccgtc tgaaccgctg tttggtggtt tcagacggca
     2221 tttgccgtaa aaatgcccga aaacctgttt cgggcatgga ttcggactta atttactttt
     2281 ttgatgtcga cttgagccgg ctgcttggcg ggcgcgtttt cgggtgcgcc gattttgacc
     2341 agtttcacat caaataccaa agtggcgttc gggccgattt tgtcgcccgc accctgttcg
     2401 cggtaggcaa ggttggacgg gatgtagaac gtggcttcgc cgccttcttt cagaagctgt
     2461 acgccttcgg tccaacccgg aatcacttgg ctcaaaggga aggtgaccgg gccgccgttg
     2521 gctttgctgc tgtcgaatac cgtaccgtca atcaggcggc cttcgtattc cacggtaacg
     2581 atgtcgtctt tggacggctg tttgccttca ccctgtttgg tgattttgta ttgcaggccg
     2641 gaagcagtgg tcttcacgcc gtctttggcg gcattttctt tcagaaaggc ttcgcctttt
     2701 tctttattgg ccttcgcgtc cgccttgtgt ttttctacgg ctttagcctg ttgttcctga
     2761 aggaatttca tcatgacttc ctgagcctgc tcttcagtca ttttgatttc tttgccgtca
     2821 tacactgcct gcatggcttc ggtaaagact ttcaaatcga tttccgcacc ctgttccttc
     2881 atttgcttca gggagcgtcc gatgtccacg cccatcgcat agcttgcctg ctgcatcgtg
     2941 ctgccgatcg aagaggtgtc gccctgcgcg gcagaagcgg cggcaggttc ggatgcggct
     3001 tcttttttgc cacaggcgga aagtgccaaa gcggcggaaa gggtcagtgc gctgattttg
     3061 aaaatggtgt tcatgatgga tcttcgctgt cgataaggtc ggaaaaacgg gattatagcc
     3121 gagtttgaat gtttcaacac acaggatgac acataaagcg tcaatcgtgt gttgccctgt
     3181 tttggaaggg attgaacctt ccaaaataag ttttgattct accgccccga gggacagatg
     3241 tccaagtggc ggggttcaac cgataaggaa attttaatca aatagaatca agcctgttta
     3301 aattttgtaa atgcggcatt tcagacggca ttttatgcct tgccctccat gccgtgatgt
     3361 tcgatggcaa aaccgcttcg gcggtaggcg gtaaagcgtt cgcgcgcgtc ggcgagatct
     3421 tccaagctgt tgccgacgat ttccaaaacg cgcgcgggca agaccggagc ggtgttccaa
     3481 aaaccgtcgg acaggttcaa aacagtcatg ccttcgggaa ttcggggcgg attgccgccg
     3541 caagcaagca ggacggatgt ttcagacggc atggcttctt ccatttccca aatttcgtgc
     3601 ggaatgaaac tctcggcttc gtattgccaa agcattttgt ccaattcctg aagccgcccg
     3661 aacgaatcgg accacaccag tatcctgccg ccgtcccgaa tcgcacgggc aatcaggcgg
     3721 caggtgaaaa tcggaacttg ggcaacgtgc gtgtaaaagg tggctttcgg catattgttt
     3781 gagcatttgg caggacgatg ccgtctgaaa ggcttcagac ggcattgtgg caaaattaaa
     3841 gattccgcag atagttcagc agcaagggaa cgggacggcc agtcgcacct ttttccgctc
     3901 cggatttcca cgccgtaccc gcgatgtcaa ggtgcgccca cgggtagcct tcggtgaagt
     3961 aagacaggaa tgttgcggcg gtaatcgtgc ccgcgccggg cgtgccgata ttggggatgt
     4021 cggcaaagtt ggatttgagc tggtctttgt aggtttcaaa gagcggcagt tgccacgctt
     4081 tgtcgtccac gttgtaggaa gcggcaagca ggctgtcgac caaatcttga ttgttgccca
     4141 tcacgccgct gacatcgtgc cccaaggcga caatacacgc gccggtcagg gtggcgacgt
     4201 cgatgacggc tttgggtttg aactgttcgg cgtaagtgag cgcgtcgcac aaaatcagac
     4261 ggccttcggc atcggtgttc aacacttcga tggtcagacc tttcatactt ttcacgacat
     4321 cgcccggttt gtttgccgcg ccggaaggca tattttcaca agtggcgacg acggcaatca
     4381 ggttaatcgg cagttgcagt ttgacggcgg cgcagaaggt gctgatgacg gttgccgcgc
     4441 cgcacatatc aaacttcatt tcgtccatgt tcaggccggg tttgagggaa atgccgccgg
     4501 tgtcgaaggt aatgcctttg ccgaccaata ccacaggcgc ggcttctttg tccgccgcac
     4561 cgaaatagct cagttcgacc aaatagggat cttcgacact gcctttggcg accgaccaaa
     4621 acgaacccat gttttctttg atgtagtctt tttcgatgat tttggcgtgc gcgcccagtt
     4681 tttcggcttc ggctttggcg gtgcgcgcta aaaattcggg cgtgcattcg ttgggcgcgg
     4741 cgttgcccaa gtcgcggcag aggctttgtc cgtaaacttg cgcttcggcg acgcgcaagg
     4801 cttctttaac gacggcttcg tgtgcgctgt ggaacacggc ggtttcaaat ttggcgggct
     4861 tggcttcttt tttgtagcgg tcgaaacggt aggcggcgtt gccgaacgca atcgcaaacg
     4921 cttcggctac ggcggcggct tgcgcttcat cgaaggcatg gatgtccaca ttgaccgttt
     4981 cctgattttg cgcccatttg gcggcttcgg cggcggcctt gttcaatgcg gcgcggccgg
     5041 tgcttttcag acagcatacg gcaacagcct gcaaaccgtt gcctgtcggg atttttgtgt
     5101 cggcaaaatt ttgaccttct tcaagcgaag acaaaagggc aagggcggtc ggatggttta
     5161 attgcgcctc ttcggtgcag acaaataact gtgcgcctgc ctgctgttcc tgcaagattt
     5221 cggtttttgt gctaaattcc acgtttattc tcctgattga gacggttgtc ggtagttttc
     5281 ggacggcctt tcgctcaaaa gaccgtctga agacggctgg cacgattgta ccccatttga
     5341 agcaccgtct gaaaccttgc gcggacaatc cgcctgcgcc gaaccgctta ccgccccctg
     5401 accgcgattc tatgatttat caaagaaacc tcatcaaaga actctctttt accgccgtcg
     5461 gcattttcgt cgtcctcttg gcggtattgg tctccacgca ggcaatcaac ctgctcggcc
     5521 gtgccgccga cgggcgtgtc gccatcgatg ccgtgttggc attggtcggc ttctgggtca
     5581 tcggtatgac gccgcttttg ctggtgttga ccgcatttat cagtacgttg accgtgttga
     5641 cccgctactg gcgcgacagc gaaatgtcgg tctggctatc ctgcggattg gcattgaaac
     5701 aatggatacg cccggtgatg cagtttgccg tgccgtttgc cgttttggtt gccgtcatgc
     5761 agctttgggt gataccgtgg gcagagctac gcagccgcga atacgctgaa atcctgaagc
     5821 agaagcagga attgtctttg gtggaggcag gcgagttcaa cagtttgggc aagcgcaacg
     5881 gcagggttta ttttgtcgaa accttcgata ccgaatccgg catcatgaaa aacctgttcc
     5941 tgcgcgaaca ggacaaaaac ggcggcgaca acatcatctt cgccaaagaa ggtaacttct
     6001 cgctgaacga caacaaacgc acgctcgaat tgcgccacgg ctaccgttac agcggcacgc
     6061 ccggacgcgc cgactacaat caggtttcct tccaaaaact caacctgatt atcagcacca
     6121 cgcccaaact catcgacccc gtttcccacc gccgtaccat tccgaccgcc caactgattg
     6181 gcagcagcaa cccgcaacat caggcggaat tgatgtggcg catctcgctg accgtcagcg
     6241 tcctcctact ctgcctgctt gccgtgccgc tttcctattt caacccgcgc agcggacata
     6301 cctacaatat cttgattgcc atcggtttgt ttttaattta ccaaaacggg ctgaccctgc
     6361 tttttgaagc cgtggaagac ggcaaaatcc atttttggct cggactgctg cctatgcaca
     6421 tcatcatgtt cgtcatcgca atcgtacttc tgcgcgtccg cagcatgccc agccagccct
     6481 tctggcaggc ggttggcaaa agtctgacat tgaaaggcgg aaaatgaacc tgatttcacg
     6541 ttacatcatc cgtcaaatgg cggttatggc ggtttacgcg ctccttgcct tcctcgcttt
     6601 gtacagcttt tttgaaatcc tgtacgaaac cggcaacctc ggcaaaggca gttacggcat
     6661 atgggaaatg ctgggctaca ccgccctcaa aatgcccgcc cgcgcctacg aactgatgcc
     6721 cctcgccgtc cttatcggcg gactggtctc tctcagccag cttgccgccg gcagcgaact
     6781 gaccgtcatc aaagccagcg gcatgagcac caaaaagctg ctgttgattc tgtcgcagtt
     6841 cggttttatt tttgctattg ccaccgtcgc gctcggcgaa tgggttgcgc ccacactgag
     6901 ccaaaaagcc gaaaacatca aagccgccgc cattaacggc aaaatcagca tcggcaatac
     6961 cggcatttgg ctcaaagaaa aaaacagcat tatcaatgtg cgcgaaatgt tgcccgacca
     7021 taccctgctg ggcattaaaa tctgggcccg caacgataaa aacgaactgg cagaggcagt
     7081 ggaagccgat tccgccgttt tgaacagcga cggcagttgg cagttgaaaa acatccgccg
     7141 cagcacgctt ggcgaagaca aagtcgaggt ctctattgcg gctgaagaaa actggccgat
     7201 ttccgtcaaa cgcaacctga tggacgtatt gctcgtcaaa cccgaccaaa tgtccgtcgg
     7261 cgaactgacc acctacatcc gccacctcca aaacaacagc caaaacaccc gaatctacgc
     7321 catcgcatgg tggcgcaaat tggtttaccc cgccgcagcc tgggtgatgg cgctcgtcgc
     7381 ctttgccttt accccgcaaa ccacccgcca cggcaatatg ggcttaaaac tcttcggcgg
     7441 catctgtctc ggattgctgt tccaccttac cggacggttc ttcgggttta ccagccaact
     7501 ctacggcatc ccgcccttcc tcgccggcgc actacctacc atagccttcg ccttgctcgc
     7561 tgtttggctg atacgcaaac aggaaaaacg ttgaaccaat gccgtctgcc ccccttcgga
     7621 cggcattggt ttttattatc atattcccgc agacagatag ccgttccct
//
LOCUS       DAWKST010000096         7650 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.096, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000096 DAWKST010000000
VERSION     DAWKST010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.096"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(127..1989)
                     /gene="hscA"
                     /locus_tag="WJL87_001393"
     CDS             complement(127..1989)
                     /gene="hscA"
                     /locus_tag="WJL87_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ7799815.1"
                     /translation="MALLQISEPGMSASPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTY
                     YLPYRFGDNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDGAITRMLKDSMDNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAKELQQI
                     RQGIADLQGRLKDGKAEDIRSAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            2160..3032
                     /gene="hpnD"
                     /locus_tag="WJL87_001394"
     CDS             2160..3032
                     /gene="hpnD"
                     /locus_tag="WJL87_001394"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ7799816.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCHCVAGVVGCLIARILGFSDDQTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMRRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPDADKKAQKVGLVMAAVYYALLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            3429..4742
                     /locus_tag="WJL87_001395"
     CDS             3429..4742
                     /locus_tag="WJL87_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEZ7799817.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARALAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRTLPLPAPLHILGGVLLARRVPSAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPCAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKAHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFASAEACLQSLSDAV"
     gene            4825..5544
                     /locus_tag="WJL87_001396"
     CDS             4825..5544
                     /locus_tag="WJL87_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ7799818.1"
                     /translation="MATLSDKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGYPEPFAICFDLISAEEKEFEHFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASTESKGRSGEIVYL"
     gene            5709..6380
                     /locus_tag="WJL87_001397"
     CDS             5709..6380
                     /locus_tag="WJL87_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEZ7799819.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVEKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            6390..6758
                     /locus_tag="WJL87_001398"
     CDS             6390..6758
                     /locus_tag="WJL87_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ7799820.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            6755..7402
                     /locus_tag="WJL87_001399"
     CDS             6755..7402
                     /locus_tag="WJL87_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ7799821.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
BASE COUNT         1707 a         2196 c         2117 g         1630 t
ORIGIN      
        1 acgcccttga tgacggtttt catcgaatag acaatgcctg acttttccat atcgatgttt
       61 ttcaagtgtt ttcgagcctt cagacggcat caggttatat ctatgccgtc tgaaaccgtt
      121 taagtatcaa atattatcga cactctggcc tgtcaatgcg cgttggatat tgcggttcat
      181 gcgtttggcg gcgaagttgt cggtgctgcg gctgagtttg gcgacggcgg agcggatgtc
      241 ttcggctttt ccgtctttca gacggccttg caaatcggcg atgccttgcc ggatctgttg
      301 caattctttg gcatccagta aatcgctgtc caactcgagg gcggcgttga cggcatcggt
      361 caggctttcg gcttcgacta cggcttcggc gcgggcgcgt gccgccatat cttcggcggc
      421 gttgtccata ctgtctttaa gcattcgggt aatcgcgccg tcgtccaagc cgtaggaagg
      481 tttgacttcg atttgcgcct gtacgccggt gctttgttct tgggcggaaa cggacagcag
      541 cccgtccgca tcgatttgga aggttacgcg gatacgcgcc gcacccgccg ccatcggtgg
      601 aatgccgcgc agggtgaatt tggcaaggct gcggcagtca gaaacgagct cgcgctcgcc
      661 ttgcacgacg tgtatcgtca tcgcggtttg accgtctttg aaggtggtaa agtcctgcgc
      721 gcgcgcggtg gggatggtgg aattgcgcgg gatgattttt tccgccaagc cgccgtaggt
      781 ttccaaaccg agcgacaaag gtgtaacgtc cagcagcagc cattcgccgt cggttttgtt
      841 gcctgcgagg acgtttgcct gtatggcggc gccgagcgcg acgacttcgt cgggattgag
      901 gttgttcagc ggggtttgtc cgaagaaggt ggcgactgcc tgttggacgt gcagcatacg
      961 ggtcgaaccg ccgaccataa tcacgccttt gacttcgttt ttaccgacac cggcatcttt
     1021 caacgcctgt gtgaccggtt cgagcgtttt catcaccaaa tgctgcgtca ggttgtggaa
     1081 ctcggcgcga ctgatgcttg tgtcgattgc catgccgtct gaaagcgtcg cctgaatgcg
     1141 cgcttcggtt tgcgtggtta attgttcttt ggcggcgcgg acgagcgaga gcaggagttg
     1201 gctgtcttgt tcgttgagtt gggagagtcc gttttgttcg agcaggcggc agaacaggcg
     1261 gtggtcgaaa tcgtcgccgc ccaacgcgct gttgccgccg gtggctttga cttcaaacag
     1321 tcctttggtc agttgcaata cggatacgtc gaatgtgccg ccccctaagt cgtacacgac
     1381 aaacgtgcct tccgaggcgt tgtccagccc gtaggcgatt gcggcagcgg tgggttcgtt
     1441 gagcaggcgc aatacgttca aacccgccag acgcgcggca tctttggtgg cttggcgttg
     1501 ggcatcatcg aaataggcag ggacggtaat caccacgccg accaaatcgc cgcccaaggt
     1561 ttcttcggcg cgcgatttaa gggttttgag gatttccgcc gacacttcga caggcgtttt
     1621 caccccctgc cgcgtatgca gttcgataac gcgttgattg tcgccgaaac ggtaaggcag
     1681 gtaatacgta ttttgatgca gatcggcaag cgtccgcccg ataaggcgtt tggcggagct
     1741 gacggtgttc agcgggtcgg ttttttgggc ggacagggcg gttttgccga cttcaatgcc
     1801 gccgttttcc agatagcgga cgacggaagg cagggtaacg cgcccttcgg catcgggcag
     1861 acaggcggca ctgccgctgc ggacggtggc gaccaagctg ttggtcgtac ccaaatcgat
     1921 gcctgctgcc aaacggtgcc ggtgcgggga ggcggacatg cccggttctg aaatctgcaa
     1981 aagagccata aattctgcct gtcggtgcgg tgtgtaaagg aaggaagtcg gcattttaac
     2041 agaatttgcg ccgatagttg agcaaaccgc taaggattgg gtcgtgccgc cgaaattgcc
     2101 ctctaccgcc aaatgccgat aaaatccgcc tttcccgccc atcattagga atataaatcg
     2161 tgaaaggact cgactattgc cgccaaaaag cagaagaaag ccgctccagt tttttgtcgg
     2221 gcttccgttt cctgactcag gaaaaacgag atgcggtaac ggttttatat gctttttgcc
     2281 gcgaattgga cgatgtggtt gacgaatgtt ccaaccccga tgttgcacag gcgacattga
     2341 actggtggcg cggcgatttg gacaaggtat tcggcggcgc gatgccggaa caccccgtca
     2401 atcaggcctt gcggcaggtt aaggaaacct tcaagctgcc gaaatatgaa ctggaagcct
     2461 taatcgacgg gatgcagatg gatttggttc aagcccgtta cggcagtttt gaagaattga
     2521 aactgtattg ccactgcgtc gcaggcgtgg tcggctgcct gattgcgcgg attttggggt
     2581 tttcagacga ccaaacgctg gaatacgccg acaagatggg acttgcgctg caactgacca
     2641 acatcatccg cgatgtcggc gaagacgcgc gcaggggacg gatttacctg ccgatggagg
     2701 aaatgcggcg gtttgacgta cccgcaagcg tgattttgca atgcagcccg acgggcaatt
     2761 ttgccgaatt gatggcgttc caaatcaaac gcgcccgcga aacctaccgc gaagccgtat
     2821 cgctgctgcc tgatgccgat aaaaaagccc aaaaagtcgg actggtcatg gcggcggttt
     2881 attacgcgct tttaaacgaa atcgaccgag acggcgcgca aaacgtcctc aaatacaaaa
     2941 tcgccctccc ttcgccgcgc aaaaaacgca ttgccctgaa aacctggtta ttcggattca
     3001 aaccgcgccc cggcacgccg gaacgggcat aaggcgcata ccgcccgccg cccgtcaggc
     3061 aaacccgatt cccacagccc gccggacggg gtttcaacca tcgtctgaaa gtctgcatcg
     3121 taaccgcgca atcccgttgg cgttcgcgct ttgaaatcga attgctggga atttaaacag
     3181 gagcagaagt aaatgaaagc agtcagggat atagccttat ggctggcagt aacggtttgg
     3241 atcaactttt tccccgacaa ttaccgctat gataccgttc cacagggacg gtacggatac
     3301 tggcatgcag accatccgtg gtatccctat gtgcgctttc tgctgccttt atttttagcc
     3361 tgtatccttt tctaccgtca ttttcggaaa cggaaataga tacggctgca aaaccattgg
     3421 aggaataaat gatgaacacg ccgcatccgc gcccgaaaat cgccgtcatc ggcgcaggct
     3481 gggcaggact gtccgccgcc gtcaccttgg cgcggcacgc cgacgttacc ctgtttgaag
     3541 ccggccggca ggcgggcggc agggcgcgcg cactggccgg aaataccgac ggtttcggtt
     3601 ttttggacaa cgggcagcac attttgctcg gcgcataccg gggcgtgttg cgcctgatga
     3661 aaaccatcgg ttcagacccc cgtgccgcct ttttgcgcgt accgctgcac tggcatatgc
     3721 acggcggttt gcagttccgc accctccccc tgcccgcgcc gctgcatatt ttgggcggcg
     3781 tgctgcttgc ccggcgcgta ccgtccgcat tcaaagccaa actgcttgcc gatatgtccg
     3841 atttgcagaa gtccgcacgc ctcggacagc ccgacacgac agttgcacaa tggctgaaac
     3901 agcggaacgt gccgtgtgcc gccgtgatgc agttttggca gccgctggtg tggggtgcgc
     3961 tcaacacgcc tttggaaacc gcaagcctgc gcgtgttgtg caacgttttg tccgacggcg
     4021 tgctgacgaa aaaatccggc agcgactatc ttctacccaa gcaggatttg ggcgcaatcg
     4081 tcgccgaacc cgccttggcg gagcttcaac ggctcggcgc ggacatccgc ctcgaaacgc
     4141 gcgtatgccg tctgaacacc ctcccggacg ggaaagtgct cgtcaacggc gaagctttcg
     4201 atgccgccgt ccccgccacc gcgccctacc acgccgccgc gctcctgccc gaaggcacgc
     4261 ccgaacacgt tcagacggca tatcaaaacc ttcgctacca cgccatcacc accgtctatc
     4321 tgcgctacgc cgaacccgtc cgcctgcccg ccccgctgac cggccttgcc gacggcacgg
     4381 tgcaatggct gctttgccgg ggcaggctcg gactgcctga aaacgaagtg tccgccgtca
     4441 tcagcgtttc cgaccgcgtc ggcgcgtttg caaaccgggc gtgggcggac aaagcccacg
     4501 ccgacctcaa acgcatcctt ccgcatttgg gcgaacccga agccgtgcgc gtcatcaccg
     4561 aaaaacgcgc cacaaccgca gccgatgccc cgccgccgga cttgtcgtgg ttgcaccggc
     4621 accgcatctt ccccgccggc gactacctcc acccggacta ccccgccacg ctcgaagccg
     4681 ccgtacaatc aggtttcgcg tcggcggaag cctgcctgca aagcctgagc gatgccgtct
     4741 gaaaacgccg gccggtatcg ggacgcttta caaggtgcgg caaaacgcta aaatacctta
     4801 accgaccacc aaaaaggaaa aagtatggcg acactgtccg acaaaaccat cttagttacc
     4861 ggcgcatcgc aaggtctggg cgaacaggtc gccaaagcct atgcggcggc aggcgcaacc
     4921 gtgattctgg tggcacgcca tcagaaaaaa ctggaaaaag tctatgacgc gattgtcgaa
     4981 gccggatacc ccgaaccatt cgccatctgc tttgacctta ttagcgcgga agaaaaagaa
     5041 tttgaacatt tcgccgccac cattgccgaa gccacgcaag gcaaactgga cggcatcgtc
     5101 cactgcgccg gctattttta cgccctctcg ccgctggatt tccaaaccgt cgccgaatgg
     5161 gtcaaccaat accgcatcaa caccgtcgca cctatggggc tgacccgcgc cctgttcccg
     5221 ctgctgaagc agtcgcccga cgcgtccgtc atcttcgtcg gcgaaagcca cggcgaaaca
     5281 cccaaagcct actggggcgg cttcggcgcg tccaaagccg cgttgaacta cctgtgcaaa
     5341 gtcgccgccg acgaatggga acgcttcggc aacctgcgcg ccaacgtcct cgtccccgga
     5401 cccatcaatt ccccgcaacg catcaaatcc catccgggcg aagccaaaag cgaacgcaaa
     5461 agctacgggg acgtgctgcc cgcatttgtc tggtgggcaa gtaccgaaag caaagggcgg
     5521 agcggcgaaa tcgtttacct ctaaatccgg aaaaggcagg catcccgccc gcgccgcgat
     5581 tccgtttgaa aaatatccgt accttccgcg cgcatccgtc ccgcacagac aaacaccttg
     5641 acttaaattt acataaggac aataatgata acccgttatt tccttaactt gaccttaggg
     5701 taaattttat gaaaaccgtt tccaccgccg ttgtccttgc cgccgctgcc gtttcactga
     5761 ccggctgtgc gaccgaatcc tcacgcagtc tcgaggtaga gaaagtcgcc tcctacaata
     5821 cgcaatatca cggtgttcgt accccgattt ccgtcggaac attcgacaac cgctccagct
     5881 tccaaaaagg cattttctcc gacggggaag accgtttggg cagccaggca aaaaccattc
     5941 tagtaacgca cctgcaacag accaaccgct tcaacgtact gaaccgcacc aatttgaacg
     6001 cattaaaaca ggaatccggc atttccggca aagcgcataa cctgaaaggc gcagattatg
     6061 tcgttaccgg cgatgtaacc gaattcggac gcagagatgt cggcgatcat cagctcttcg
     6121 gcattttggg tcgcggcaaa tcgcaaatcg cctatgcaaa agtggctctg aatatcgtca
     6181 acgtcaatac ttccgaaatc gtctattccg cacagggcgc gggcgaatac gcactttcca
     6241 accgtgaaat catcggtttc ggcggcactt ccggctacga tgcgactttg aacggcaaag
     6301 ttttagactt ggcaatccgc gaagccgtca acagcctggt tcaggctgtt gacaacggcg
     6361 catggcaacc caaccgttaa ggagaaatga tgaatccgaa aaccttgagc cgtttgtcgc
     6421 tgtgtgcggc ggtcttggct ctgaccgcct gcggcggcaa cgggcaaaaa tccctgtatt
     6481 attacggcgg ctatcccgat accgtctatg aaggtttgaa aaacgacgac acttcgttgg
     6541 gcaagcagac cgaaaagatg gaaaaatact ttgtggaagc cggcaacaaa aaaatgaatg
     6601 ccgccccggg tgcgcacgcc catctgggac tgctgctttc ccgttcggga gacaaagagg
     6661 gcgcgttccg ccagtttgaa gaagagaaaa ggctgtttcc cgaatcgggc gtatttatgg
     6721 acttcctgat gaaaacaggt aaaggaggca agcgatgaaa cctttgattt tagggcttgc
     6781 cgccgtgttg gcgctgtctg cctgccaagt tcaaaaagcg cccgatttcg actacacgtc
     6841 attcaaggaa agcaaaccgg cttcaatttt ggtggttccg ccgctgaacg aatcgcccga
     6901 tgtcaacgga acatggggtg tactggcttc gaccgccgcg ccgctttccg aagccggcta
     6961 ttacgtcttc cccgccgcag tcgtggagga aaccttcaaa caaaacggct tgaccaatgc
     7021 cgccgatatt cacgccgtcc ggccggaaaa actgcatcag attttcggca atgatgcggt
     7081 tttgtacatt acggttaccg aatacggcac ttcatatcaa attttagaca gcgtgacgac
     7141 cgtatccgcc aaagcacggc tggtcgattc ccgcaacgga aaagagttgt ggtcgggttc
     7201 ggccagcatc cgcgaaggca gcaacaacag caacagcggc ctgttggggg ctttggtcag
     7261 cgcagtggtc aatcagattg ccaacagcct gaccgaccgc ggttatcagg tttccaaaac
     7321 cgccgcatac aacctgctgt cgccctattc tcacaacggc atcttgaaag gtccgagatt
     7381 cgtcgaagag cagcccaaat aagcatatgc cgtctgaacc tctccgagct tcagacggca
     7441 tatgcgaatc ttgatacttg caagccgcac cgcccaaaat cgggcggccg gtttcaatat
     7501 ccgaacaagg ttcgggttct gtattttgca gaacccgaac cttttattca aatttaaact
     7561 taattccggc gtggttgtat ggtatagtgg attaacaaaa atcaggacaa ggcgacgaag
     7621 ccgcagacag tacaaatagt acggaaccga
//
LOCUS       DAWKST010000097         7541 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.097, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000097 DAWKST010000000
VERSION     DAWKST010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7541
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.097"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            24..419
                     /locus_tag="WJL87_001400"
     CDS             24..419
                     /locus_tag="WJL87_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ7799822.1"
                     /translation="MFGLHKSFGFLTLTVITLRIVWAVANRAKRPQSDSKAAAAGHGI
                     LYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGWLLF
                     AAVVGHVAMVVVHRVQGKDVLYRMTGRVR"
     gene            complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="WJL87_001401"
     CDS             complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="WJL87_001401"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ7799823.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREVGFKVVPVVGASAVMAALSVAGVAGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPVVMFETPHRIGATLADMAELFSERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYNLALSWKNK"
     gene            1376..1723
                     /locus_tag="WJL87_001402"
     CDS             1376..1723
                     /locus_tag="WJL87_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ7799824.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GSRPPEWIQNITG"
     gene            1728..2321
                     /locus_tag="WJL87_001403"
     CDS             1728..2321
                     /locus_tag="WJL87_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ7799825.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAGKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFNHVF
                     SKQVRALGRAGDILVGISTSGNSANVIEAIKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPHPRTARIQENHILLIHAICDCIDSVLLEGM"
     gene            2385..2993
                     /locus_tag="WJL87_001404"
     CDS             2385..2993
                     /locus_tag="WJL87_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ7799826.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            3001..3651
                     /locus_tag="WJL87_001405"
     CDS             3001..3651
                     /locus_tag="WJL87_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799827.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRAVLLICI
                     TAIGALAIGIVSTFNPNGDKTLQAEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            3691..4470
                     /gene="map"
                     /locus_tag="WJL87_001406"
     CDS             3691..4470
                     /gene="map"
                     /locus_tag="WJL87_001406"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ7799828.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGSVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            4625..4924
                     /locus_tag="WJL87_001407"
     CDS             4625..4924
                     /locus_tag="WJL87_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ7799829.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5155..5529
                     /locus_tag="WJL87_001408"
     CDS             5155..5529
                     /locus_tag="WJL87_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ7799830.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(5717..7183)
                     /locus_tag="WJL87_001409"
     CDS             complement(5717..7183)
                     /locus_tag="WJL87_001409"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ7799831.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSTPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLCAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         1946 a         2286 c         1844 g         1465 t
ORIGIN      
        1 aagaggcgga atgggcgggc agcctgttcg gcctgcacaa atctttcggc ttcctcacgc
       61 tgacggtgat tacattgcgc atcgtgtggg cagttgccaa ccgcgccaag cgtccgcaaa
      121 gcgacagcaa ggctgcggcg gcaggacacg gcattctgta tctgctcatg cttgccgttc
      181 ccgttatcgg catgatccgc caatacggca gcggccgcgg cccgttgaaa gtgttcggcg
      241 ttgaggtgat gcagggttcg ccggaaaaaa tcgagtggat ggcaaacttg ggcaacacgt
      301 tccacggcaa tttgggctgg ctgctgtttg ccgccgtcgt cggacacgtc gccatggtcg
      361 tcgtccaccg tgttcaaggc aaagacgtgc tgtaccgcat gacggggcgt gtccgttgat
      421 tccgttcgca ctatggtgcc ggtccgtccg gcatcatttg tttttccaag acagtgccag
      481 attgtacaaa gctttttttc cctcgcccgt gattttggcg gcaagctccg ccgcctgttt
      541 ggtcggcagc tcggctgtga ggattttcat gatgttttgc gcggactcgg acaagccttc
      601 gtgtttttca tcctgcgccg gataaagcac caacaccatc tcaccgcgcg attggttgcc
      661 gtcggcagac aatgccgtct gaatttcccc aaccgtgccg cttaagaacg tttcaaacgt
      721 tttcgtaatt tcgcgcgcca gcattaatcg gcgttcggag aacagttccg ccatatcggc
      781 aagcgtcgca ccgatgcggt gcggcgtttc aaacatcacg acgggaaacg ccgcccgcac
      841 ccatttggca aacagtttcc tgcgctctcc cgatttcggc ggtacaaaac cgttgaaata
      901 aaaatcggat cccgccacac cggccacact caaagccgcc atcaccgcgc ttgcgccgac
      961 aacagggaca actttaaacc cgacctcacg cacgcggcgg gcgagtttcg cgcccgggtc
     1021 gcacacggcc ggcgtacccg catcggaaac ctgtgccaca accatgccgt ctgaaagata
     1081 gccgacaatc ttgtccgcca tctgccgttc gttgtgttcg cgcacgctga caagtttgcc
     1141 ctgaatgccg tacgcgctca aaagctgcgc ggtaacgcgc gtgtcttcgg cacagatgat
     1201 gtccgccttt tgcaataccg ccaaagcgcg cagggtaatg tccgccaaat tgccgatggg
     1261 cgtggcaacc acgtataatg tccctccgac gacgctgtcg gaggctttct gcaagtgttt
     1321 ctgaaacata aaaaatgccg tctgaaaaac agacattata aaggttaaac cgattatgcg
     1381 cctaaaccac aaacagggcg aggcagggga agatgccgcg cttgccttcc tccaatccca
     1441 aggctgcaca ctgcttgccc gcaactggca ctgcgcctac ggcgaaatcg acctgattgt
     1501 caaaaacggc ggcatgattc tgtttgttga agtaaaatac cgcaaaaatc ggcaattcgg
     1561 cggtgccgca tacagcattt ccccatccaa attattgaaa ctgcaacgaa gtgtagagta
     1621 ttatctgcaa cagaacaggt tgacaaacgt accgtgccgc ctcgacgcgg tactcatcga
     1681 aggcagccgc ccgcccgagt ggatacagaa tattacaggt tgacgatatg acgacattac
     1741 aagaacgcgt tgccgcccat tttgccgaaa gcatccgtgc caagcaggaa gccggaaaag
     1801 tattggtcga gccgaccgta caggctgccg agctgatgct gcaatgcctg atgaacgacg
     1861 gcaagattct ggcgtgcggc aacggcggtt cggctgccga cgcgcagcac ttcgccgccg
     1921 aaatgaccgg acgctttgaa aaagagcgca tggaactcgc cgccgtcgcc ctgaccacag
     1981 acacctccgc gctgaccgcc atcggtaacg actacggctt caaccacgtc ttcagcaaac
     2041 aggtacgcgc cctcggacgc gccggcgaca tcctcgtcgg catttccact tccggcaatt
     2101 ccgccaacgt catcgaagcc attaaagcgg cacacgaacg cgatatgcac gtcatcgccc
     2161 tgaccggccg cgacggcggc aaaatcgccg ccatactcaa agacaccgac gttctgctca
     2221 acgtccccca tccgcgcacc gcccgcattc aggaaaacca catcctgctg atacacgcca
     2281 tatgcgactg tatcgactcc gtactgctgg aaggaatgta acccttttca gacggcatgg
     2341 cgcaaagcaa tgccgtctga aacgcccaag aaaggaagcc cccgatgaaa cccaaaccgc
     2401 acaccgtccg caccctgatt gccgccattt tcagccttgc ccttagcggc tgcgtcagcg
     2461 cagtaatcgg aagcgccgcc gtcggcgcga aatccgccgt cgaccgccga accaccggcg
     2521 cgcaaaccga cgacaacgtt atggcgttgc gtatcgaaac caccgcccgc tcctatctgc
     2581 gccaaaacaa ccaaaccaaa ggctacacgc cccaaatctc cgttgtcggc tacaaccgcc
     2641 acctgctgct gctcggacaa gtcgccaccg aaggcgagaa acagttcgtc ggtcagattg
     2701 cacgttccga acaggccgcc gaaggcgtat acaactacat taccgtcgcc tccctgccgc
     2761 gcactgccgg cgacatcgcc ggcgacactt ggaacacatc caaagtccgc gccacgctgt
     2821 tgggcatcag ccccgccaca caggcgcgcg tcaaaatcgt tacctacggc aacgtaacct
     2881 acgttatggg catcctcacc cccgaagaac aggcgcagat tacccaaaaa gtcagcacca
     2941 ccgtcggcgt acaaaaagtc atcaccctct accaaaacta cgtccaacgc tgactcggca
     3001 atgccgtctg aaccgccttc agacggcatt gcccgacacc ccaaaagcac aatcaaaatg
     3061 gcaaaaaaac cgaacaaacc cttcaggctg acccccaaac tcctgatacg cgccgtattg
     3121 ctcatctgta tcaccgccat cggcgcattg gcaataggca tcgtcagcac attcaacccg
     3181 aacggcgaca aaacccttca agccgaaccg caacacaccg acagcccccg cgaaaccgaa
     3241 ttctggctgc caaacggcgt agtcggacaa gatgccgccc aacccgaaca ccaccacgcc
     3301 gcctcatccg aacccgcaca gccggacggc acagacgaaa gcggcagcgg actgccgtcc
     3361 cctgccgcac ccaagaaaaa ccgggtcaaa ccgcaacctg ccgacacagc tcaaaccgac
     3421 aggcagccgg acgacgccgg aacacaagct gaaaacacac tcaaagaaac ccccgtactg
     3481 cccacaaacg tcccccgtcc cgaaccccga aaagaaacac ccgaaaaaca ggcgcagccc
     3541 aaagaaacac ccaaagaaaa agaaacgccc aaagaaaacc ataccaaacc ggacaccccg
     3601 aaaaacacgc cgcccaaacc ccataaagaa attctcgaca acctcttctg acccggcacg
     3661 gcaggcacac ccgcaatcca aggaagcatt atgaacggca tcatcatcaa aacccccgaa
     3721 gaaatcgaaa aaatgcgcga gctgggcaaa ctcgtcgccg aagccctcga ctacatcgga
     3781 caattcgtca aacccggcgt aaccaccgac gaaatcgaca aactcgttta cgactaccac
     3841 gtcaacgtcc aaggcggcta tcccgccccc ctgcactacg gcaacccgcc ctaccccaaa
     3901 tcctgctgca cctccgtcaa ccacgtcatc tgccacggca ttcccgacga caagccgctc
     3961 aaagaaggcg acattatcaa catcgacctc accatcaaaa aagacggctt ccacggcgac
     4021 tccagccgta tgtttaccgt cggcaaagtc tcccccatcg cccaacgcct gatcgacgta
     4081 acccacgcct ccatgatggc gggcatagaa gccgtcaaac ccggcgcgac actgggcgac
     4141 gtaggttacg cctgccaaca ggttgccgaa aacgccggct attccgtcgt acaggaattc
     4201 tgcggacacg gcatcgggcg cggtttccac gaagccccgc aagtgttgca ctacggaaaa
     4261 aaaggacagg gctccgttct aaaaccgggt atgattttta ccgtcgaacc gatgatcaac
     4321 caaggcaaac gccacctgcg tatcctcaac gacggctgga cggtggttac caaagaccgc
     4381 tccctctccg cccaatggga acacgaagtc ttggtgaccg aaaccggcta cgaaatcctc
     4441 accgtcagcc ccgcctccgg caaaccctga agccggacgt atccgcccca taaaaacaaa
     4501 caatgccgtc tgaaagaaac ggcagatatg atatataata taaaaacagg cttgacccgg
     4561 cacattacga aaacaaagca aatcggaatt tgccccgcaa ccagacaaac ttaaaggaag
     4621 ttttatgaaa atatttgaaa atatagaaga tgttaaagcc atccgtaaaa agaccgggct
     4681 gaaccagata gacttctggg gcaaggtcgg cgttacccag tctggaggct cgcgctacga
     4741 aaccggccgc aaaatgccca aacccgtacg cgaactgctc cgcctcgtcc atatcgaatg
     4801 catcgatttg gcgaaagtca acaaaaaaga tatggaaatc gccgccctgt tgaaaaaaca
     4861 ccatcccgac ctatatgccg agttgtccaa acagaccaag tccgaaagaa aaaaacaaag
     4921 ttaaaccgca acctccggat gcccgacagt ttttcatttc cgaaaaacgc aaacaatgcc
     4981 gtctgaaaca ccggacaggt cgccgtatgc cgcccctgcc tcaaaccgcc gaaccgcccg
     5041 aacccgcctt tttacaaact ttatccaatt tcctgtttat ttcaggatac gccgacatta
     5101 gaatgtcaaa cagctcgaaa cgggcaaact ccacatccat ccaaaggaat aaaaatgaaa
     5161 cttctgacca ccgcaatcct gtcttccgca atcgcgctca gcagtatggc tgctgctgcc
     5221 ggcacgaaca accccaccgt tgccaaaaaa accgtcagct acgtctgcca gcaaggtaaa
     5281 aaagtcaaag taacctacgg cttcaacaaa cagggtctga ccacatacgc ttccgccgtc
     5341 atcaacggca aacgtgtgca aatgcctgtc aatttggata aatccgacaa tgtggaaaca
     5401 ttctacggca aagaaggcgg ttatgttttg ggtaccggcg tgatggatgg caaatcttac
     5461 cgcaaacagc ccattatgat taccgcacct gacaaccaaa tcgtcttcaa agactgttcc
     5521 ccacgttaat caggcaacaa aaaacagcgt tttcagaaat gaaaacgctg tttttttgac
     5581 cgttccatta ttcgcaaaag ggaaaaaacg attacctgcc ccgtgtatca aaacctgccc
     5641 tgccggatga agggcataac cggcagggac ggcatcaaca ccatatgggg gtacggcttt
     5701 tcttgaaaga ttcggcttaa atatccaaca ctttcgcggt ataggcgata atttcatccg
     5761 ccctttcggg gttttcgttc aacttgatgc cgtaacccgg taccagctct ttcagacggc
     5821 cttcccaaga cggggcgcgc tcggggaagc attggtgcat cagccggatc atcagcggca
     5881 cagcagtcga tgcgcccggc gacgcgccca gcaatgcggc gagtgagccg tcggcgtggg
     5941 cgacaatctc cgtaccaaac tggagcacgc cgcctttttc ggagtctttt ttaatgattt
     6001 ggacgcgttg ccctgcggtg atgagttccc agtcgtcggg gtttgcctcg gggtagtatt
     6061 ccagcaggga ggcgaagcgt tcttctttgg ttttacgcaa ttcgcccagc aggtatttgg
     6121 tcagcggcat attcgcccag ccggcgcaca gcataggata gaggttgtcc atatggatgg
     6181 acagcggcaa atccataagc gagccttgct tgaggaagtt ggaacggaag cctgcataag
     6241 ggccgaacat aaggtggcgt ttgccgtcca cgttgcgtgt gtcgaggtgc gggacggaca
     6301 tcggcggcgc gccgacggaa gcctgcccgt acactttggc gttgtgttgt tcggcggttt
     6361 caggggtgct gttgcggaag aacaggccgg acacggggaa gccgccgtag cctttgcctt
     6421 cggggatgcc ggatttttgc agcagggtca gcgcgccgcc gcccgcgccg aggaagagga
     6481 agcgggtacg gagggtgagc tgcccgtcgg ggttgcgggt atcggcggtt ttgagcaccc
     6541 acgcgccgtc ggattcgcgt ttgatgtctt cgacgtggcg gttgaactcg gtttttacgc
     6601 ccttgccctg caaatatttc accatttggc gcgtcagccg tccgaaatcg acatccgtgc
     6661 cttcggcgga gtagttggcg gcgacgggtt ggttttcgtc ccggccgcgc atcatcagcg
     6721 gagcccaatc ggaaattttg ttccgatcgg tggaaaattc catattttca aaaagttttt
     6781 gggttttaaa cgcgtcataa cgtttttgaa gataagaaca atggtcttca ttcatcacca
     6841 aagacatatg cggcacggca ttgatgaagg aattgtcttc caacttgcct tccgcgacca
     6901 acgtcgccca aaactggcgg ctgacatgaa actgttcggc aatattgagg gcgcgcgccg
     6961 gatcgataat cccatttgca cccaacggcg catagttcaa ttcgcacagc gcggaatgcc
     7021 ccgtgccggc gttgttccac gcgtttgacg attccaacgc cacatcttcc aagcgttcaa
     7081 tcagggtgat ttcccaagac ggttcgagtt ctttgagcaa aacgcccaaa gtcgcgctca
     7141 taatgccgcc gcccaccaag acaacgtctg tcgcttcagc catggtttac tcctaaaaaa
     7201 caggcatctt ctgcccttat ggttatttgc cgtactacaa acgcctgaat cgcaaaagca
     7261 gggaaaaccg gcaatggtgt gtgtccgagt atgctgtttc ggggttggaa tgcgttgcaa
     7321 gtatggcttc cgacaccgct tcaggggctt gtaatatgtt atcgtgaatg tagtggattt
     7381 tactgggaaa tgcaaagttt ttctgtcgcc cgccaagccc gggaactgcg aaatgaaaaa
     7441 taaaaatagt tatttatcta tatatatcaa atttttaata gataaaaaac caaaattgtt
     7501 tatatattaa tttttaaaag attgtcagca tattgcgtta a
//
LOCUS       DAWKST010000098         7528 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.098, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000098 DAWKST010000000
VERSION     DAWKST010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7528
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.098"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            26..2041
                     /locus_tag="WJL87_001410"
     CDS             26..2041
                     /locus_tag="WJL87_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ7799832.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLENDKARTLS
                     DGILAQMQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCAAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEKPDRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNQVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIDKDAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSAEGEAKPLNLPNDCDVVGYLAGHLLL
                     TLRKDWNRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEVELPRLPSGALEMTDQPWGGDVVYLAAGDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGIEVRQFWVMSSDGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGVPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPQHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEVCKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQREAADELACVLLFLKEFLG"
     gene            complement(2155..3111)
                     /locus_tag="WJL87_001411"
     CDS             complement(2155..3111)
                     /locus_tag="WJL87_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7799833.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRSFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            3347..4312
                     /locus_tag="WJL87_001412"
     CDS             3347..4312
                     /locus_tag="WJL87_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7799834.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTRSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGSRQLIQAAEQLKEAFEKAEPVAAGKE"
     gene            4856..5179
                     /locus_tag="WJL87_001413"
     CDS             4856..5179
                     /locus_tag="WJL87_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ7799835.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSLVLIELSD
                     PSEAV"
     gene            5293..7470
                     /locus_tag="WJL87_001414"
     CDS             5293..7470
                     /locus_tag="WJL87_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ7799836.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLDADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPASFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDLSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT         1683 a         2282 c         2175 g         1388 t
ORIGIN      
        1 acctgcccaa ccctcaagga ccccgatgaa atcctacccc gacccctacc gccattttga
       61 aaacctcgat tccgccgaaa cgcaaaactt cgctgctgaa gcgaatgccg aaacgcgcgc
      121 gcgtttttta gaaaacgaca aggcgcgcac gctgtcggac ggcattttgg cgcagatgca
      181 ggacacgcgg cagattccgt tttgtcagga acaccgcgcg cggatgtacc atttccatca
      241 ggacgcggag tatccgaagg gcgtgtaccg cgtgtgtgcg gcggcgacgt atcgttccgg
      301 ctatcccgag tggaaaatcc tgttttcagt ggcggatttc gacgagctgc tcggcgacga
      361 tgtgtatttg ggcggcgtgt cgcacttggt ggaaaagccc gaccgtgcgt tgttaacact
      421 gagcaaatcg ggcggcgata cggcgtacac gctggaagtg gatttggaag caggggagtt
      481 ggtagaaggc ggttttcact ttccggcagg caaaaaccaa gtgtcgtggc gcgatgaaaa
      541 cagcgtgtgg gtgtgtccgg cttgggacga acgccagttg accgaatcgg gctatccgcg
      601 tgaggtgtgg ctggtggaac gcggcaagag tttcgaggaa agcctgcctg tgtatcaaat
      661 cgataaagat gcaatgatgg tgaacgcgtg gcgttatctc gatccgcagg gttcgccgat
      721 tgatttgatt gaagcgtcgg acggttttta caccaaaacc tatttgcagg tgtcggccga
      781 aggcgaggcg aaaccgttaa acctgcccaa cgattgcgat gtggtcggct atctggcggg
      841 gcatcttttg ctgacgctgc gcaaggactg gaaccgcgcg aaccaaagct atccgagcgg
      901 tgcgctggtg gcggtgaagc tgaatcgggg tgaactcggg gcggcgcagc ttttgtttgc
      961 gcctgatgaa acgcaggcat tggaaagcgt ggaaacgacc aagcgttttg tggtggcgag
     1021 cctgctggaa aacgtacaag gccgtctgaa agcgtggcgt tttgccgacg gcaaatggca
     1081 ggaagtcgaa ctgccgcgcc tgccttcggg cgcgttggaa atgaccgacc agccttgggg
     1141 cggcgacgtg gtttaccttg ccgccggcga tttcaccaca ccgctgacgc tgtttgcgct
     1201 ggatttgaac gtgatggaac tgaccgtcat gcgccgccag ccgcagcagt tcgattcaga
     1261 cggcatcgaa gtgcggcagt tttgggtgat gtcgtccgac ggcgaacgca tcccttattt
     1321 ccacgtcggc aaaaacgccg cgcccgacac gccgacgctg gtttatgcct acggcggttt
     1381 cggcgtaccc gagctgccgc attatctggg cagcatcggc aaatactggc tggaagaggg
     1441 caacgccttc gtgttggcga acatacgcgg cggcggcgag ttcggcccgc gctggcatca
     1501 ggcggcgcag ggaatcagca aacacaaaag cgtcgatgat ttgcttgccg tcgtgcgcga
     1561 tttgtccgag cgcggcatca gttcgcccca acacatcggt ttgcagggcg gcagcaacgg
     1621 cggcctgatt accgccgccg ccttcgtgcg cgaaccgcaa agcataggcg cactggtgtg
     1681 cgaagtgccg ctgaccgaca tgatccgtta tccgctgctc tccgccggtt caagctggac
     1741 agacgaatac ggcaatccgc aaaaatacga agtctgcaaa cgccggttgg gcgaattgtc
     1801 gccgtatcac aatctttcag acggcatcga ttatccgccc gcgctcatca ccaccagcct
     1861 cagcgacgac cgcgtccatc ccgcccacgc gctcaagttt tacgccaaac tgcgcgaaac
     1921 ctcgccgcaa tcttggctct actcgcctga cggcggcggc cataccggca acggcacgca
     1981 gcgcgaagcc gccgacgaac tcgcctgcgt gttgctgttt ttgaaagagt ttttgggcta
     2041 agggcgggga gcggcactgc cgccgcgaat gaaaaaaggt cgtctgaaac tgctttttca
     2101 gacgaccttt tttaatggtt gtttcaaatc aaaatatcta tgccgccggc cccatcagca
     2161 cttcttcaca tccgaaggca aaaatccgta atgccgtctg aacgcttcgt tgaaccgtcc
     2221 cgcgtggcgg tagccgcaaa agtgcatggc ggcttggacg gtgctgccgg attcgatgag
     2281 ggcgagcgcg tgttccagcc gcaggcggcg caggcatccg gcgacggttt cgccggtttg
     2341 cgctttgaaa tagcgtttca ggtagcattc gttcagtccg acgcggcggg cgatttcggc
     2401 gatggtcagc ggacgggcga attcgtgttg caggatgtcg gcggcttcgt ctatgcgccg
     2461 acggcggtaa ccgttgtcgt ggcggcggaa ggtgaagcgc aataatcggg cggagagttc
     2521 cagcgcggcg gcttcgtcgg caagcaggcc gaagccgtcc gattcgaacg agcgttgcag
     2581 cagtgggcag gccgccgccg tcagtgccgc tgcgttttgc gccagccgtt gcagggcgaa
     2641 tcggcctatc gtttgcggcg aaaacaggcg ttcgtccagc aagccttcgt cgtgccagcg
     2701 gcgcagtttt tccagcgaaa aatccaaatg cagcgcgcac atgccgctgt tgtcgggcag
     2761 cagggtttcg gatacgtccg ccaactcgcc gcgtaccagc cagatttcgc cggcagacgg
     2821 gcggtattcc ctgccgccca tttgtaaccg gttctgcccc gacaccatga cgaacaaggc
     2881 gcagttgtgg ctgaaattgt ggatttcggt gggaaacgcg cccgttccgc cgccgcgcat
     2941 ccgcgacaag gtgatgcccg agtcgaagcg gttgatgcac atttccagat gcaaaccggg
     3001 ctgttttgcc tgcgcaatga gcgcgctgtc ggaacagccg tccaacgccc agccggattt
     3061 atcggagcgg acataggttt ggtactggcg gtagatggcg gcggtgttca tgattggata
     3121 ggaacgagtt gtctaacaaa tgaattaaat aggaattatt accaataatc aagcgcaggg
     3181 attggttgaa acggaaaagg tcgtctgaaa gggtgtttca gacgaccttt tccgtatcgg
     3241 gaatttgttt tgccgtatcg ggaattttgc gttttgcggc gtggtttctg caggttgttt
     3301 gcttaataat aaacattctt attcgtatgc aaaggaaccg cacaccgtga aaccgcgttt
     3361 ttattgggca gcctgcgccg tcctgctgac cgcctgttcg cccgaacctg ccgccgaaaa
     3421 aactgtatcc gccgcatccg catctgccgc cacactgacc gtgccgaccg cgcggggcga
     3481 tgccgttgtg ccgaagaatc ccgaacgcgt cgccgtgtac gactgggcgg cgttggatac
     3541 gctgaccgaa ttgggcgtga atgtgggcgc aaccaccgcg ccggtgcgcg tggattattt
     3601 gcagcctgca tttgacaagg cggcaacggt ggggacgctg ttcgagcccg attacgaagc
     3661 cctgcaccgc tacaatcctc agcttgtcat taccggcggg ccgggcgcgg aagcatatga
     3721 acagttggcg aaaaacgcga ccaccataga tctgacggtg gacaacggca atatccgcac
     3781 cagcggcgaa aagcagatgg agaccttggc gcggattttc ggcaaggaag cgcgcgcggc
     3841 ggaattgaag gcgcagattg acgcgctgtt cgcccaaacg cgcgaagccg ccaaaggcaa
     3901 aggacgcggg ctggtgctgt cggttacggg taacaaggtg tccgccttcg gcacgcggtc
     3961 gcggttggca agttggatac acggcgacat cggcctaccg cctgtagacg aatctttacg
     4021 caacgagggg cacgggcagc ctgtttcctt cgaatacatc aaagagaaaa accccgattg
     4081 gattttcatc atcgaccgta ccgccgccat cgggcaggaa gggccggcgg ctgtcgaagt
     4141 attggataac gcgctggtac gcggcacgaa cgcttggaag cgcaagcaaa tcatcgtcat
     4201 gcctgccgcg aactacattg tcgcgggcgg ctcgcggcag ttgattcagg cggcggagca
     4261 gttgaaggag gcgtttgaaa aggcagaacc cgttgcggcg gggaaagagt agggagtcgt
     4321 ctgaaaacgg agtttccgaa ggaagcgggg ggtttctgcg aagctaaagt gcggtttcaa
     4381 cgaattgaaa agcagcctgt atgttgaaaa taccgctcaa gcaaacctac ggtttgccgc
     4441 cctctcccta tccctctccc acagggagag gggattgggg tgcaggctgc ctttaaggtt
     4501 taggcaaatt tttaacttcg ttgaagctgc gatttcagaa gctccgtttt agcttcgcag
     4561 aaactccgct tccttcgaaa gctccgtttt cagacgacct tttggagtac cgcaggcaca
     4621 cgcatcgaac ggctgaatca aagttcagac cgatggcagc ccgcacccga gtttatgcgg
     4681 caaacagcga ggctacggca acccgtcccc tctccctgtg ggagagggtt agggagaggg
     4741 cggtaagccg caggcttaca tcaaagccga taacgcttcc gttacaactc cgcccactga
     4801 acgaagccaa aacgacaaac cgcatcgtaa accacccaac ccataggaga accccatgca
     4861 aaacgaaacc atcaacctga aacagcacct tgccgccatc aaagaatact ggcagcccga
     4921 aatcatcaac cgccacgggt tccaattccg tttggtcaaa cttttgggcg attacggctg
     4981 gcatacgcac ggatacagcg acaaagtgct gtttgccgtg gagggcgaca tggcggtgga
     5041 cttcgccgac ggcggcagca tgacgatacg cgagggcgag atggcggtcg tgccgaagtc
     5101 ggtgtcgcac cgcccgcgtt cggaaaacgg ctgctcgttg gtgctgattg agttgtccga
     5161 cccgtccgag gccgtctgaa aacgaagttt ccgaaggaag ctgagtttct gcgaagctaa
     5221 aagcagcctg caccttcaat caatatgccg aaaatacaac cccaccgcac accaacacac
     5281 aaaggaaatc ccatgacacg cttcaaatat tccctgctgt ttgccgccct gttgcccgtg
     5341 tacgcgcagg ccgatgtttc tgtttcagac gaccccaaac cgcaggaaag cactgaattg
     5401 ccgaccatca ccgttaccgc cgaccgcacc gcgagttcca acgacggcta cactgtttcc
     5461 ggcacgcaca ccccgctcgg gctgcccatg accctgcgcg aaatcccgca gagcgtcagc
     5521 gtcatcacat cgcaacaaat gcgcgaccaa aacatcaaaa cgctcgaccg cgccctgttg
     5581 caggcgaccg gcaccagccg ccagatttac ggctccgacc gcgcgggcta caactacctg
     5641 ttcgcgcgcg gcagccgcat cgccaactac caaatcaacg gcatccccgt tgccgacgcg
     5701 ctggccgata cgggcaatgc caacaccgcc gcctatgagc gcgtagaagt cgtgcgcggc
     5761 gtggcggggc tgctggacgg cacgggcgag ccttccgcca ccgtcaatct ggtgcgcaaa
     5821 cgcccgaccc gcaagccatt gtttgaagtc cgcgccgaag cgggcaaccg caaacatttc
     5881 gggctggacg cggacgtatc gggcagcctg aacaccgaag gcacgctgcg cggccgcctg
     5941 gtttccacct tcggacgcgg cgactcgtgg cggcagcgcg aacgcagccg cgatgccgaa
     6001 ctctacggca ttttggaata cgacatcgca ccgcaaaccc gcgtccacgc aggcatggac
     6061 taccagcagg cgaaagaaac cgccgacgcg ccgctcagct acgccgtgta cgacagccaa
     6121 ggttatgcca ccgccttcgg cccgaaagac aaccccgcca caaattgggc gaacagccgc
     6181 caccgtgcgc tcaacctgtt tgccggcatc gaacaccgct tcaaccaaga ctggaaactc
     6241 aaagccgaat acgactacac ccgcagccgc ttccgccagc cctacggcgt agcaggcgtg
     6301 ctttccatcg accacaacac cgccgccacc gacctgattc ccggttattg gcacgccgac
     6361 ccgcgcaccc acagcgccag cgtgtcattg atcggcaaat accgcctgtt cggccgcgaa
     6421 cacgatttaa tcgcgggtat caacggttac aaatacgcca gcaacaaata cggcgaacgc
     6481 agcatcatcc ccaacgccat tcccaacgcc tacgaatttt cccgcacggg cgcctatccg
     6541 cagcctgcat cgtttgccca aaccatcccg caatacggca ccaggcggca aatcggcggc
     6601 tatctcgcca cccgtttccg cgccgccgac aacctttcgt tgatactcgg cggcagatac
     6661 agccgttacc gcaccggcag ctacgacagc cgcacacaag gcatgaccta tgtatccgcc
     6721 aaccgtttca ccccctacac aggcatcgtg ttcgacctga ccggcaacct gtcgctttac
     6781 ggctcgtaca gcagcctgtt cgtcccgcaa tcgcaaaaag acgaacacgg cagctacctg
     6841 aaacccgtaa ccggcaacaa tctggaagcc ggcatcaaag gcgaatggct tgaaggccgt
     6901 ctgaacgcat ccgccgccgt gtaccgcgcc cgtaaaaaca acctcgccac cgcagcagga
     6961 cgcgacctga gcggcaacac ctactaccgc gccgccaacc aagccaaaac ccacggctgg
     7021 gaaatcgaag tcggcggccg catcactccc gaatggcaga tacaggcagg ttacagccaa
     7081 agcaaaaccc gcgaccaaga cggcagccgc ctgaaccccg acagcgtacc cgaacgcagc
     7141 ttcaaactct tcactgccta ccactttgcc cccgaagccc ccagcggctg gaccatcggc
     7201 gcaggcgtgc gctggcagag cgaaacccac accgaccctg ccacgctccg catccccaac
     7261 cccgccgcca aagcccgcgc cgccgacaac agccgccaaa aagcctacgc cgtcgccgac
     7321 atcatggcgc gttaccgctt caatccgcgc gccgaactgt cgctgaacgt ggacaacctg
     7381 ttcaacaaac actaccgcac ccagcctgac cgccacagtt acggcgcact gcggacagtg
     7441 aacgcggcgt ttacctatcg gtttaaataa ggtcgtctga aaacggagtt tctgcgaagc
     7501 tatagtggat taacaaaaac cagtacgg
//
LOCUS       DAWKST010000099         7464 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.099, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000099 DAWKST010000000
VERSION     DAWKST010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7464
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.099"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(16..771)
                     /locus_tag="WJL87_001415"
     CDS             complement(16..771)
                     /locus_tag="WJL87_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ7799837.1"
                     /translation="MKFARLLFLCAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCTSR"
     gene            complement(898..2043)
                     /gene="nirK"
                     /locus_tag="WJL87_001416"
     CDS             complement(898..2043)
                     /gene="nirK"
                     /locus_tag="WJL87_001416"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
                     /protein_id="HEZ7799838.1"
                     /translation="MKRQALAAIIASMFALAACGGEPAAQTPAASAEAASSAAQTAAE
                     TPSGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYHYWTFDGD
                     VPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTSTFSFK
                     ALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKGKKGA
                     QGLQPFDMDKAVAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLVSSFH
                     VIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRAFNKG
                     ALGQLKVEGAENPEIMTQKLSDTAYAGSGAAPAASAPAASAPAKSDY"
     gene            2413..4669
                     /locus_tag="WJL87_001417"
                     /pseudo
     CDS             2413..4669
                     /locus_tag="WJL87_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
     gene            4848..5381
                     /locus_tag="WJL87_001418"
     CDS             4848..5381
                     /locus_tag="WJL87_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ7799839.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(5444..6628)
                     /locus_tag="WJL87_001419"
     CDS             complement(5444..6628)
                     /locus_tag="WJL87_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ7799840.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRF
                     QRFAGTDAQRVADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(6668..7072)
                     /locus_tag="WJL87_001420"
     CDS             complement(6668..7072)
                     /locus_tag="WJL87_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ7799841.1"
                     /translation="MTAQPCPICTAQDEDILLQTPKLRVIAVHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFQPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
BASE COUNT         1651 a         1815 c         2082 g         1916 t
ORIGIN      
        1 ctataattga aggggttatc ggcttgtgca acggaagccc aagttgtgca agacatattt
       61 ggattgcagg ctggtgcgga tgccgtagcg gaggaaggcg gcatagttgg acgagtcgct
      121 cgacccgata gacgcgccgc tgcaaaacat ttgcgcgttg gcattgccgg aagaaagcag
      181 gctgctgttg aaatcttccg tccattccca aatcagcccg tgcatatcat aaacgcccca
      241 gtagttcggg cggcctttgc cgacatcgtg caggcctttc cgtccgccgt cggcatacca
      301 atcgagaata gtgcggttgt agccgggttc gtttgagccg tttttctgcg tggcggaagc
      361 aagtccggca aattcccatt cgtcaatggt cggcaggcgt ttgccttgtg cggcgcaata
      421 ggcgttggcg gcaaaccagg aaacatttgt taccggttgt tttaattcgc ccgccttcgg
      481 cgcatagctg cggctgccgt ttttcatcca atgcttcagg taagcgggtt ctgcctgttt
      541 ggaaccgatc ctgccttttt gccattgggg gtggctgttg acaaattcgg caaactcggc
      601 attggtaacg ggatatttat ccagtttgaa tggtttgact ttaatcaggc cggtatcttt
      661 tttcagataa agcgggcggt agctgccgcc ttcgatttga accatttcgg cagctgccgc
      721 ttgagtgccg gcgagtgccg cacagagaaa gagtaaccgg gcaaacttca tatcatgcct
      781 cctgacaggc agttaaaatc aatcttccga aaggaaagat tggttgttaa aaaaccactg
      841 ccgtgcgtaa tgaagtacgg cggcagtggt tcgtttgcct aaaaatgggt gttcaattta
      901 gtaatcgctt tttgcaggag cagaagctgc gggagcggaa gcagcaggag ctgcaccgct
      961 gccggcgtaa gcggtatcac tcaatttttg agtcataatt tcagggtttt ccgcaccctc
     1021 tactttcaat tggcccaacg cgcctttgtt gaatgcgcgg aagatggagt ggtcgaccaa
     1081 agtgtagctg cccgggatgt cgactttgaa ttcgacgatg gcagagccgc cggcaggaac
     1141 gatggtgctt tgtacgtttt cgttgatcag tttgccgcct tcaacataaa ctttgtcgaa
     1201 gatttcgccg atgacgtgga aggaagacac caggttcgga ccgccgttac cgacgtacat
     1261 acgcacggtt tcgcctgctt tggctttcag cgcgttatcg ccggcgatag cacctacatg
     1321 accgttgaat acgacgtatt caggctgttc ggcaacggct ttgtccatat cgaacggttg
     1381 caggccttgc gcgccttttt tgcctttggt gtagaagtcg ccttggacga tgtagaactc
     1441 tttatccact ttcggcagac cttctttagg ctcgaccaaa atcagaccgt acataccgtt
     1501 ggcgatgtgc ataccgaccg gtgcgacggc gcagtggtag atgtacagac ccggttgcag
     1561 ggctttgaag ctgaatgtgg aagtacggcc cggagcggta aaggttgcgg ccgcgccgcc
     1621 gccctggccg gtagccgcgt ggaagtcgac gttgtgcggt acggtggaag aaggattgtt
     1681 ggaaaattcc acttcaaccg tatcgccttc gcgtacgcgg atcatgcggc ccggaacatc
     1741 gccgtcaaat gtccagtaat ggtattccac gccgtcgtcc attttcatgg ttttttcgac
     1801 ggtttccatt tttacgcgga ctttggcagg atagtcgcgg tcgattgcgg gaggtacttc
     1861 gggagcgtgg gtggtaaccg catcgataac gggcaactcg ccggacggtg tttcggcggc
     1921 ggtttgtgcg gcggagcttg cggcttcggc ggaagcggca ggggtttgcg cggcaggttc
     1981 gccaccgcag gcggctaaag caaacataga agcgataatt gcggctaagg cttggcgttt
     2041 cataatgttt tccttttgta agaaaagtag ggagattggt taagaactgg tgtgattatg
     2101 cgtttgcccc atcaccgaca ttttgattta agtcaaataa ttcataaaat atgaacttaa
     2161 aatgatactt ctaatacttt atagctacta aaaattacaa taaataggct gatttgctta
     2221 agggcttcgg caaatcaggc aaaggcaggg tagttgccga ctaaagaata atgatagtta
     2281 ttatcatata tgtatttgtt ttatatgtga gtttctttga acaaatgttt atttgcgcgg
     2341 taaaccgtgc tacaatcttt aacattcata ttttgtgaat tttaatccac tatattaaaa
     2401 ggagctctca aaatgggaca gtacaagaag ctgtggtact tgctgtttgc cgttctggcg
     2461 gtatgcttta ccattcttgg ctatatgggc agcgaggttt ataagaaagc cccgccttac
     2521 cccgaacagg tcgtttccgc atcaggcaaa gtgctgatga cgaaagacga tattttggca
     2581 ggtcagtctg cgtggcagag taccggcggt atggaagtcg gttcgattct gggtcacggc
     2641 gcatatcagg ctccggactg gacggcggac tggctgcatc gtgagttgtc cgcttggttg
     2701 gatttgaccg cgcaacagac ttacggcaaa aaattcgatg aagtttcccc tgaagaacaa
     2761 gccgttctga aaacccgact ggctgacgaa taccgcaacc aaagccgtat taaagaagac
     2821 ggcagcgtcg tcatcagcga tacgcgcgtg aaagccatcg aaagcatcct gccttattac
     2881 cacggtgttt acggcgatga tcccgcgctg caaaccaccc gcgaacactt tgcaatgaaa
     2941 aacaacacat tgccaagcca agaagcgcgt gaaaaactgt tcgacttctt cttctggact
     3001 tcttggtctg cttcgaccaa ccgtcctgac gagacgttca cttataccaa caactggccg
     3061 cacgagcctt tgatcaacaa cgtaccgacc actgaaaact atatgtggtc attcaccagc
     3121 gttgttcttc tgctgatggg catcggtttg ctgatgtggg gttattcctt cttgaccaaa
     3181 cacgaggaag tggaagtgcc gtctgaagac ccgatttcca aaatccaact gaccccttcg
     3241 caaaaagcat tgggcaaata cgtcttcctg acggtcggcc ctgtttgtgg tacaagtatt
     3301 gctgggcgga ctgaccgcgc actacaccgt cgaaggccag ggtttctacg gcattgacga
     3361 ggcgttgggc tttgaaatgt ccgactggtt cccctacgcc ctgacccgta cttggcacat
     3421 ccaatccgcc atcttctgga ttgcaaccgg ctttttgacg gcaggtttgt tcctcgcgcc
     3481 gattgtcaac ggcggtaaag atcccaagtt ccaacgtgcc ggcgtaaact tcctctatat
     3541 cgccctcttc atcgtggtcg gcggttctta cgcgggcaac ttcttcgcgt tgacgcacat
     3601 ccttcccccg gaatttaact tctggttcgg acaccaaggt tacgaatacc tcgatttggg
     3661 acgtttctgg caactccttt tgatggtcgg tctgctgtta tggctgttcc tgatgttgcg
     3721 ttgcacggtt tccgccttta aagaaaaagg cgtggacaaa aacctgctgg caatctttgt
     3781 cgcttctatg gtcggtgtgg gcgtattcta cgcaccaggc ctgttctatg gcgaaaaatc
     3841 cccgattgct gtaatggaat actggcgttg gtgggtggtt cacctgtggg tggaaggctt
     3901 ctttgaagta tttgccactg ccgcttttgc ctttgtcttc tacaatatgg gctttgtccg
     3961 ccgcagtacc gccacagctt ctactctggc cgctgccgcc atctttatgt tgggcggcgt
     4021 tccaggtacg ctgcaccacc tgtacttctc cggctctacc tccgcctcta tggcaatcgg
     4081 tgcctgcttc tccgctttgg aagtcgtgcc gctggtgttg ctgggccgtg aagcatacga
     4141 gcattggtct taccaacacc tgtccaaatg ggcgaaacgc ctgcgttggc cgctgatgtg
     4201 cttcgtggca gtcgcctttt ggaatatgat cggtgccggc gtattcggct tcctgatcaa
     4261 tccgccgatt tccctgttct atattcaagg tttgaacact tctgccgttc acgcgcacgc
     4321 cgccttgttt ggtgtgtacg gtttcttggc attgggcttc gtattgttgg ttgcccgtta
     4381 tttgaaaccg aacgcgcagt ttgatgacaa actgatgact tggggcttct ggctgctcaa
     4441 cggtggcttg gtcggtatga ttgttatcag cctgctgcct gtcggcgcga ttcaggcata
     4501 cgcctccatc acgcacggtc tgtggtatgc ccgcagcgaa gagttcctgc aaatggaaat
     4561 cctcgatacc ttgcgttggg tacgtacggc agctgacttg gtctttatcg gcggtgcaat
     4621 ctgcgttgcc atccaagcta ctaaaatcgt attcggccgg gacaaataag ttttgcggta
     4681 tgaaatgagc aactgcaccc gttttcgggt gcagtttttt attgcggatt gggacgggaa
     4741 tggaaaagga tgtggatttt ttattttaat cggctataca attgctgctt tcatgccgtc
     4801 tgaagcacgt ttcagatggc atcggtttac atcagataag gataaaaatg ggtatttacg
     4861 attttcaaat gaaagatgcg gaaggcaatg cggttgattt gtcaggctat cgcggcaagg
     4921 ttctgctgat tgtcaacacg gcaacgcgtt gcggtctgac cccgcaatac gaggctttgc
     4981 agaagctgta tgcacaatat accgcagaag gcttggagat tttggatttt ccgtgcaacc
     5041 agttccgcga acaggctccc gaaagcagcg gagaaattgc ccaagtgtgt atgatgaagt
     5101 ttggcacgaa gtttaagatt ttcgacaaaa tcgaagtcaa tggagcaaat accgcgccct
     5161 tgtatgccta cctgaaatcc gtcaaaccgc aagataaagg caatcatctg tttaaagatt
     5221 tcgtgttgaa gctcgcagct ttgggagaga agcgcgacga aggcgacatc aaatggaact
     5281 ttaccaaatt cctcgtcaac cgcgacggcg aagtggtcga gcgttttgcg cctagtgtta
     5341 cgcccgaaga aatcgaagcc gatattcggg cattgctgta atctgccgcc gtcttgggaa
     5401 atgccgtctg aaggcttcag acggcatttg tccgtttgcg ggtttattcc gaaatatcgg
     5461 caacaccgct ttcggggaag atgggcaaat ccggcggtgg gagcagggta tccaaagtca
     5521 gggcggacgc atcgagtgtg gggtagcctt caaacgcgac cgaataaccg ctgtttccgt
     5581 tcatacggat tcgggcgtgc gcgcccagag ggaaagtgat tttgtcggca atgtgcccga
     5641 agggaaagcc cgtcagcacg gggattttcg ccgtgcgcga aacatggttg gcaacggcgg
     5701 aaaaatcata agacggatca tagacatctc gaattttttc catacggaaa ttgccgaaca
     5761 cgatggcgcg ctgtttcttc aaaatacccg aaagatacag cgtattgagc atacgttcga
     5821 tgcggtaggg ctgttcgccg acatcttcga ggaacaaaat gccgccgtcg atgtcgggca
     5881 tataaggcgt gccggcgagc gaggcgagga cgcttaagtt gccgccccac aatatgcctt
     5941 cggtttcgac atcggcgcgt tggatataag gaacatcaac ggtcaggcgg ttttgggttg
     6001 cacccttgat aaaggcatcc atcgtaaacg caccgggggc gggtttgcca aaatcgctat
     6061 aagccatcgg gccggcaaaa ctcatcatat tgccttttgc caacaatgcc agctggacgg
     6121 cgcatacgtc gctgaatccg aaaaacagcg tgccgtgttc gcgcatcctt gcgccgagcg
     6181 aagcaaaatc gatatgcggc agaatccgcg ccgcaccgta accgccgcgc aagcccatca
     6241 gcactttagg cgtggcgacg cggccggaag cgacctcttg gaaatcggcg acacgttgtg
     6301 cgtccgtacc agcaaaccgt tggaagcggc ggctgcccgc ctgttggttg gttacggtaa
     6361 aaccggcatt gtaaaggcgg gttaaggctg tgttgacgcg gttggtgtct tcggcaaagc
     6421 ctgacgaagc gacaacgcgc agaaggttgc cgtccgaact ttgacggcgt ggcgtttgga
     6481 gaggcacggt tcgggctttc acaacagaat gggaagaggg aaggggcgga acggatgtgg
     6541 cggatgtgcc gcaagcctga agcagtcccg cgccggcggc ggcggtgcag gttttcagaa
     6601 aacgacggcg ggaggtcggt tcggtcataa tttccctttc atacggtttc agacggcatc
     6661 ggcgggctta aagcagcttt ttaagctgtt ccgtccaatc ttgcggcagg gtcataccgt
     6721 gttttcggac ggggtttgcc caaatcggcg cagggaagga cgcatcgttt tcaaagcggg
     6781 cgataatatg ccaatgcagg tgcggcacga cattgcccaa gctggcgagg ttaatcttgg
     6841 caggttgaaa cacttggcgc atagcggctt cgactttgta caccatttcc atcaattcgc
     6901 cgcgttccgc tgccgaaagg tcggtcattt cggcaatatg cttacgccaa atgacgcggc
     6961 agaatgcagg cgaaccgctg tcgttatgga cggcgatgac gcgcagcttg ggggtttgca
     7021 gcaaaatatc ttcatcttgt gccgtgcaga tggggcaggg ttgggcggtc atttttcctt
     7081 attcctgaaa gtgtttaaaa aacggtaggg taatttttaa tatttcaatc tgtctgtccg
     7141 cattgccgtt aattgtacca cttcataggg cggtgctcta tgtgtcgtcc tgtgtgttga
     7201 aacatatcgg acgcgtttga acagttgtgc ggcaaactgt tttagcggca ggcaagagat
     7261 gccgtctgaa gcccattgtc cgtcattccc acacaggcag ttgctacggt tgctgaagcc
     7321 ccttagccgt cgcgcaggcg gttgctacgg ttactaaaat cccgtcattc ccgcgcaggc
     7381 gggaatccag tccgttcggt ttcagttgtt tctgataaat cttgcagcgt tgggttttta
     7441 gattcccgct ttcgcgggaa tgac
//
LOCUS       DAWKST010000100         7424 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.100, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000100 DAWKST010000000
VERSION     DAWKST010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.100"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(172..450)
                     /locus_tag="WJL87_001421"
     CDS             complement(172..450)
                     /locus_tag="WJL87_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799842.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPKYNGSLWK
                     MSDGQPLRLLLAECSLKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
     gene            complement(457..741)
                     /locus_tag="WJL87_001422"
     CDS             complement(457..741)
                     /locus_tag="WJL87_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799843.1"
                     /translation="MFGGRLKNAPSITAKHFLKENIMKFINTCRKYGAKLAVVTAAPL
                     ALAAHANATLPDTAKNALEAAKADGMEAGWIVVGVFAALFVFSIVKRVMK"
     gene            complement(749..946)
                     /locus_tag="WJL87_001423"
     CDS             complement(749..946)
                     /locus_tag="WJL87_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799844.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            complement(951..1241)
                     /locus_tag="WJL87_001424"
     CDS             complement(951..1241)
                     /locus_tag="WJL87_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799845.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            complement(1286..2629)
                     /locus_tag="WJL87_001425"
     CDS             complement(1286..2629)
                     /locus_tag="WJL87_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ7799846.1"
                     /translation="MEDKQGMKKAVAGVMTDASSDGRKPATASNLPPLSNRGGTETET
                     AGRVQEVFECYEKYITDGKGNLLGVPLRRGVSDSAFIDQISFSFHEKTFFDKYGVRVS
                     LLEDEDFIRAASMLAEEVFGFCIYKESKGSGGRFYERCWLMGSEDVLYGRVHFGGQNN
                     TILFELTGTGCGVAKEGWESRLFAFLTNAIRPKITRVDIAKDFFNGEYSPNQAREDRN
                     KGMFTCHHVKPKGECLGSDWEEDDEAKMTKGKTYGIGSRESSKYVRVYEKGKQLGDKT
                     STWTRFEIEFKAKDIVIPFEVLQNPGEYFGGAYPICERFAQKATRIHAVKEDKVISAD
                     RYLEWVKKQFGRAANGLKFIFPELDKAKLFELIEPSHHKLPKSLAPEAYDCAFLKAQA
                     IHEQPAFKPYKDPYYMYEYYENLEKQLEQQKHVNNEESYNNFIYDKFARLPISWA"
     gene            complement(2714..2929)
                     /locus_tag="WJL87_001426"
     CDS             complement(2714..2929)
                     /locus_tag="WJL87_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799847.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYILRP"
     gene            complement(3217..3570)
                     /locus_tag="WJL87_001427"
     CDS             complement(3217..3570)
                     /locus_tag="WJL87_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799848.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            3806..5233
                     /locus_tag="WJL87_001428"
     CDS             3806..5233
                     /locus_tag="WJL87_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ7799849.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQSRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVVAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            5302..6705
                     /locus_tag="WJL87_001429"
     CDS             5302..6705
                     /locus_tag="WJL87_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ7799850.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(6869..7315)
                     /locus_tag="WJL87_001430"
     CDS             complement(6869..7315)
                     /locus_tag="WJL87_001430"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEZ7799851.1"
                     /translation="MDETGFDRYLFRPYARSLKGQIVKAQISGKRYQRLSLVSAQVGN
                     RLIAPMVYQNTMTGVFFEAWFQQCLLPALTQKSVIILDNARFHRMGVLREMAEKLGHK
                     VLPLAPYSPELNPIEKVWANIKRYLRTVLSDYARFDDALLSYFDFN"
BASE COUNT         1857 a         1776 c         1851 g         1940 t
ORIGIN      
        1 ttataaagtg ccaactgcgc ctgagtgata aacggcttgt tcatggttct gcctttcaaa
       61 ggtttttttg aaagcctgat tttgacacca taacttcatg cgctcaattc ttaaacagaa
      121 ccgccccgat taatacgggt acggaaacgc cgagataaaa ataaaaatcc atcatttcaa
      181 aacctttttc agcagggaaa caaagtaaac ggacgcgagg atgccgaata ctatccagcc
      241 tgtttcaagg ccgctttgca ggttgtcttt cagactgcat tccgccaata aaagccttag
      301 cggctgaccg tccgacatct tccacaggct gccgttatat ttcggcctga caatctgtcc
      361 gttttctttg attcttggta ctaccaagct gaaataaagg ttttcagcct ggtgcttctc
      421 aagacattta tttccgactt ggtagtacat gccgtcttac ttcatcactc tcttaacgat
      481 ggaaaataca aaaagcgcgg cgaaaacgcc aacgacaatc caaccggctt ccataccgtc
      541 cgcttttgcg gcttccaaag cgttttttgc tgtatcgggc aacgttgcat ttgcatgtgc
      601 ggccaaagcc aggggagcag ctgttacaac agccagtttt gcgccgtatt tacggcaggt
      661 gttaataaat ttcatgatat tttccttcaa aaagtgtttg gcggtaatgg atggagcgtt
      721 tttcagacga ccgccgaaca tccgaaaatc agtctttcaa aaatccgaat acgacaaatt
      781 cgtattggtt gccgatttct tccaaacctg cgttaatcgc ttcttcgaag tcgtagaaat
      841 aatcggcatt ggtgattaat ttggtatgtc cgatgtcgcc cgtttcagga gagagataca
      901 gaaagtcccc tgttgatacg gactggacaa catagacttt ctgcattcaa tcagcctttc
      961 ttcacgagtt gaaaaccgat gactttcagt ttttgggttt tgcccgtagt aacgatttcc
     1021 acgttcaggt ttgcttcgat gggaaattgg gcgtttcgga actgctcgaa attggcagag
     1081 ccgccgaaat cgtattcagt agtagagctg cccaatgcgt tgccttggga gctgtctaag
     1141 ggtgtggcga caatcaggcg gcaatagtcg aagctcttgc cttcgatttg tccgttgaat
     1201 tttttaacgc cgacgatgtg gccttgaagt tggatgttca ttttttggtt tccttgtgtg
     1261 attaaacgtc tttcgggcag acactttaag cccatgaaat cggtagtctt gcgaatttgt
     1321 cgtaaatgaa gttgttatag ctttcttcat tgttgacgtg tttttgctgt tcaagctgtt
     1381 tttcaagatt ctcgtaatat tcgtacatat agtaagggtc tttgtacggt ttgaatgcgg
     1441 gctgttcatg aatggcttga gctttcaaaa aggcgcagtc gtaggcttcg ggagccaaag
     1501 acttgggcag cttgtgatga ctcggctcaa tcagttcaaa cagtttggct ttgtccaatt
     1561 cgggaaaaat gaatttcaga ccgtttgccg cacgtccgaa ctgttttttt acccattcaa
     1621 ggtagcggtc ggctgaaatg accttatctt ccttaaccgc gtgtatgcgc gttgcctttt
     1681 gggcgaatcg ttcgcaaatc ggatatgcgc cgccgaaata ttcgcccgga ttctgcaaaa
     1741 cttcgaaagg gataacgatg tcttttgctt tgaattcaat ttcaaagcgc gtccatgtgc
     1801 ttgttttatc gcccaactgc ttgccttttt catagacgcg gacatatttg gacgattcac
     1861 gggagccgat accataggtc ttgcctttgg tcattttggc ttcatcgtct tcttcccaat
     1921 ctgaccccaa acattcgcct tttggtttga cgtgatgaca ggtaaacata cctttatttc
     1981 ggtcttcacg ggcttggttc gggctgtatt cgccgttgaa aaagtctttt gcgatgtcaa
     2041 cgcgtgtgat ttttgggcgg attgcattag tcaggaatgc gaaaagtcgt gattcccagc
     2101 cttcttttgc gacgccgcaa ccggtgccgg tcagttcgaa aaggatggta ttgttttgtc
     2161 cgccaaaatg gacgcgaccg tataggacgt cttccgaacc catcaaccaa cagcgctcat
     2221 agaaacgacc gcccgagcct ttggattctt tgtagataca gaaaccgaaa acttcttcgg
     2281 cgagcatgga cgcggcgcga ataaaatctt cgtcttccaa aagacttaca cgaacgccgt
     2341 atttatcgaa aaaggttttt tcatgaaatg aaaagctaat ttgatcgatg aacgccgaat
     2401 cagatacacc gcgacgaagc ggaacgccta aaaggtttcc tttgccgtct gttatgtact
     2461 tttcataaca ctcgaagact tcttgaaccc tgcccgccgt ttcggtttct gtaccccccc
     2521 tgttagataa ggggggaaga tttgaagcgg ttgccggctt cctgccgtcc gatgacgcgt
     2581 ccgtcatcac gccggcaacc gcctttttca tcccttgctt atcttccatg gtgcgaatcc
     2641 tcaaaaacgg gcaaaaaaaa gccctgttac ttgtagaaag taaaggacgt taatttttgt
     2701 taatcgtccc ttcttaggga cgcaatatat aaggttttaa aacgctaaga acaaaatccg
     2761 ataacggctc tgaagaagta agtctttctt tattgcacaa gatagcaaac ttccacggca
     2821 ttctcgcccc acccgtgccg tccaagcctt tttcaaagac atagatggtt ttcttggcaa
     2881 tcacttcgtc agtttggcgg tcgataatcc gtatggtcgc acctgccacc cagtgcctgc
     2941 gcagcttgga atcgacattg ttttcgaacg ttaccgcata acgggcgggg tgtatatgat
     3001 taaatatttg atttatcgga aaatctttac ctgaataccg aataatatcg gaatggttgg
     3061 gttgcaggac atccacatag cggtattccc catcgctaaa aaatcctagg aaacgagcaa
     3121 taaaatttac gccttcgctg gtctgtaaag ccgcattgtc ccacatcggg tctctggttt
     3181 ttgcatctgc cgaaacggta cgctcaggta ctaccttcaa cagcataatc ccttccacat
     3241 tgtccgccgt ctggtaaatc tttcccccgc cgttttgcat tgttcgttaa acacggcttc
     3301 ggcttctttg tattttctgt cccactcttc ttgtgcctgt atttcttctt tgatcgggcc
     3361 gaattgtttg ggaataatcc aaacaaacag catcaggata gcggcggcgg tcaggctgcc
     3421 tgaaaggatt ttgccggggt tccgtttggg ctttttatag gcaaagcgga cgagaaacca
     3481 aagcaacagc agcatggtgc cccaatagcc gattgagaat aggatggcca aaccttctag
     3541 gaaatggcgt aaatcgtttg tggtaaacat gggttgttcc tgtggttaaa tgtgcaggct
     3601 gctttttgcc gaaccttgcc gcatctcaaa agcagcctgc gcttcagcgt tgcgttacgc
     3661 agtaaaataa tgaatatttg taacgacttg ggtatttttt gtcaatattc cagcctttcc
     3721 cttaacagct gccgcgcttt ccgttaaaat tcctttacat atttatattg tttcctgttt
     3781 ctatattgcc aaggttatac ccgttatgtt tttctccgcc ctgaaatcct ttctttctcg
     3841 atacattact gtatggcgca atgtttgggc ggtgcgcgac cagttggaac cgcccaaacg
     3901 cacggcggaa gaacaggcgt ttttgcccgc gcatttggaa ctgaccgata cgccggtctc
     3961 tgccgctccg aaatgggcgg cgcgttttat tatggcgttt gcgcttttgg ctttgttgtg
     4021 gtcctggttc ggcaaaatcg atattgtggc ggcggcttcg ggcaaaacgg tgtcgggcgg
     4081 gcgcagcaaa accatccagc cgctggaaac ggcggtggtt aaggcggtac atgtgcgcga
     4141 cgggcagcat gtgaaacagg gagaaacgct ggcggaactg gaggctgtgg gaacagacag
     4201 cgatgtggtg cagtcggagc aggctttgca ggctgcccaa ttgtccaaac tgcgttatga
     4261 agcggtattg gcggcattgg aaagccgtac cgtgccgcat atcgatatgg cgcaagcacg
     4321 gtctttaggt ctctccgatg ccgatgtgca atcggcgcag gtgttggcgc agcaccagta
     4381 tcaggcatgg gcggcgcagg atgcgcaatt gcagtcggct ttgcgcggcc atcaggcgga
     4441 attgcagtcg gccaaggcgc aggagcagaa gctggtttcg gtgggggcga tcgagcagca
     4501 gaaaacagca gactaccgcc gtttgcgggc cgacaatttt atttcggaac atgcgttttt
     4561 ggagcagcag agcaaatcgg tcagcaattg gaacgatttg gaaagtacgc gcggtcagat
     4621 gaggcagatt caggcggcca ttgcacaggc ggagcagagt cgggtgctga atacgcagaa
     4681 cctgaaacgc gatacgctgg atgcgctgcg ccaggcaaac gaacagattg accaataccg
     4741 cggccaaacg gataaggcaa agcagcggca gcagctgatg acaatacagt cgcctgcgga
     4801 cggcacggtg caggaattgg ccacctatac ggtgggcggt gtggtgcagg ctgcccaaaa
     4861 aatgatggtg gttgcgcccg atgacgacaa aatggacgtg gaagttttgg tattgaacaa
     4921 agacatcggt tttgtggaac agggacagga tgcggtggtg aagattgaga gttttcccta
     4981 tacgcgctac ggttatctga cgggcaaggt gaaaagtgtc agccatgatg cggtaagcca
     5041 cgaacagttg ggcttggttt atacggcggt ggtgtcgctg gacaaacata ccttgaatat
     5101 tgacggcaaa gcagtgaatc tgacggcggg catgaatgtc acggcggaga ttaaaacggg
     5161 taaacggcgg gtgctggatt atctgttaag cccgctgcaa accaaattgg acgaaagctt
     5221 tagggagcga taggcggatc cgtactgggc atttgttatc cgccggttcg gacatgcaga
     5281 ctgcctgaaa ccattgcccg gatgacattg ctcaatctaa tgataatgca agattacggt
     5341 atttccgttt gcctgacact gacgccctat ttgcaacatg aactattttc ggctatgaaa
     5401 tcctattttt ccaaatatat cctacccgtt tcacttttta ccttgccact atccctttcc
     5461 ccatccgttt cggcttttac gctgcctgaa gcatggcggg cggcgcagca acattcggct
     5521 gattttcaag cgtcccatta ccagcgtgat gcagtgcgcg cacggcaaca acaagccaag
     5581 gccgcattcc ttccccatgt atccgccaat gccagctacc agcgccagcc gccatcgatt
     5641 tcttccaccc gcgaaacaca gggatggagc gtgcaggtgg gacaaacctt atttgacgct
     5701 gccaaatttg cacaataccg ccaaagcagg ttcgatacgc aggctgcaga acagcgtttc
     5761 gatgcggcac gcgaagaatt gctgttgaaa gttgccgaaa gttatttcaa cgttttactc
     5821 agccgagaca ccgttgccgc ccatgcggcg gaaaaagagg cttatgccca gcaggtaagg
     5881 caggcgcagg ctttattcaa taaaggtgct gccaccgcgc tggatattca cgaagccaaa
     5941 gccggttacg acaatgccct ggcccaagaa atcgccgtat tggctgagaa acaaacctat
     6001 gaaaaccagt tgaacgacta caccgacctg gatagcaaac aaatcgaggc catagatacc
     6061 gccaacctgt tggcacgcta tctgcccaag ctggaacgtt acagtctgga tgaatggcag
     6121 cgcattgcct tatccaacaa tcatgaatac cggatgcagc agcttgccct gcaaagcagc
     6181 ggacaggcgc ttcgggcagc acagaacagc cgctatccca ccgtttctgc ccatgtcggc
     6241 tatcagaata acctctacac ttcatctgcg cagaataatg actaccacta tcggggcaaa
     6301 gggatgagcg tcggcgtaca gttgaatttg ccgctttata ccggcggaga attgtcgggc
     6361 aaaatccatg aagccgaagc gcaatacggg gccgccgaag cacagctgac cgcaaccgag
     6421 cggcacatca aactcgccgt acgccaggct tataccgaaa gcggtgcggc gcgttaccaa
     6481 atcatggcgc aagaacgggt tttggaaagc agccgtttga aactgaaatc gaccgaaacc
     6541 ggccaacaat acggcatccg caaccggctg gaagtaatac gggcgcggca ggaagtcgcc
     6601 caagcagaac agaaactggc tcaagcacgg tataaattca tgctggctta tttgcgcttg
     6661 gtgaaagaga gcgggttagg gttggaaacg gtatttgcgg aataaagcag gctgaaacgg
     6721 ttatgaaatt cccaaagcag cctgcacccc gtttcgaaag tgcaggctgc tttgggattg
     6781 atccgatact ttcacattct cattgccttt aaataaagca cagttaaaaa cacatttaaa
     6841 ttaagtgcga attaattcaa atgtatattc aattaaaatc aaaataggac agtagtgcat
     6901 cgtcaaatcg ggcgtaatca gacaaaacgg ttcgcagata ccgcttaata ttcgcccaca
     6961 ctttctcaat cgggttgagc tcaggtgaat aaggtgcaag aggcaatacc ttatgtccca
     7021 atttttccgc catttcccgt aagacaccca tacggtgaaa tcgcgcatta tctaaaataa
     7081 tcaccgattt ttgagtcaat gcgggcagta ggcattgctg aaaccacgct tcaaaaaaga
     7141 ctccggtcat cgtattttga taaaccatcg gagcaatcag ccggttgccg acttgtgcgg
     7201 acaccaaaga taagcgttgg tatctttttc cacttatctg cgctttcact atttgccctt
     7261 tcaggctgcg ggcataggga cggaacaggt agcggtcaaa tcctgtttca tccaaataaa
     7321 cacgttggta gtcggaaaat tcggccggct gtgtcaaata atgcgttact ttggccgggt
     7381 cttgttcttt gtaagtggtg gtcttttttt gcgcgttatc ccca
//
LOCUS       DAWKST010000101         7403 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.101, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000101 DAWKST010000000
VERSION     DAWKST010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7403
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.101"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(151..573)
                     /locus_tag="WJL87_001431"
     CDS             complement(151..573)
                     /locus_tag="WJL87_001431"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ7799852.1"
                     /translation="MSIMQVEVVSSEQNIYSGEASFVVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            complement(584..1981)
                     /gene="atpD"
                     /locus_tag="WJL87_001432"
     CDS             complement(584..1981)
                     /gene="atpD"
                     /locus_tag="WJL87_001432"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ7799853.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            complement(2019..2894)
                     /gene="atpG"
                     /locus_tag="WJL87_001433"
     CDS             complement(2019..2894)
                     /gene="atpG"
                     /locus_tag="WJL87_001433"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ7799854.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYQNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            complement(2919..4466)
                     /locus_tag="WJL87_001434"
     CDS             complement(2919..4466)
                     /locus_tag="WJL87_001434"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ7799855.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            complement(4477..5010)
                     /locus_tag="WJL87_001435"
     CDS             complement(4477..5010)
                     /locus_tag="WJL87_001435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ7799856.1"
                     /translation="MAEFATIARPYAKALFGLAQEKSQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            complement(5015..5485)
                     /locus_tag="WJL87_001436"
     CDS             complement(5015..5485)
                     /locus_tag="WJL87_001436"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ7799857.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            complement(5556..5792)
                     /gene="atpE"
                     /locus_tag="WJL87_001437"
     CDS             complement(5556..5792)
                     /gene="atpE"
                     /locus_tag="WJL87_001437"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ7799858.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            complement(5849..6715)
                     /gene="atpB"
                     /locus_tag="WJL87_001438"
     CDS             complement(5849..6715)
                     /gene="atpB"
                     /locus_tag="WJL87_001438"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ7799859.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAAFGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            complement(6705..7058)
                     /locus_tag="WJL87_001439"
     CDS             complement(6705..7058)
                     /locus_tag="WJL87_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ7799860.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAVWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
BASE COUNT         1949 a         1971 c         1679 g         1804 t
ORIGIN      
        1 ctatatgttt cggttaggcg gcaggctgcc ctattgaata ccttaaagca ggctatgcct
       61 gccaacgcca tatccaaaca cagtctttaa tttaaatccg gaaaataaaa agcacgacca
      121 aacggtcgtg cttttccaaa ccaaacaagt ttatttcttg tgcgaacgga tatagtccaa
      181 agttttgagc tgtgcaatcg cagcagccaa tgctttatgc gcctccgcca aagccttatc
      241 gtctttagct tgggaaatgc ccgcttctgc ggcttttttc gcctcttccg cacgtgcctg
      301 atccatctcc gcactgcgga cggcaacatc cgccaagaca gttactttat caggctgtac
      361 ttccaaaaca ccgccggaaa cagcaaccaa aacctcttta tcctcgcccg gaacggtcaa
      421 acgcaaagcc cccggccgca ccaaactcat aatcggctcg tgtcgcgggt aaataccaag
      481 ctcaccttga acggtcggaa ccactacaaa gctggcttcg cccgaataga tgttttgctc
      541 gctactgacc acctcaactt gcatgatgct catgccgacc tcctcagttt aaggttttcg
      601 ctttctcaac cgcttcttcg atgctgccga ccatgtagaa tgcctgttcg ggcagatgat
      661 cgtattcgcc gttcaagatg gctttgaagc cggcaatggt atcgcgcagg gcgacatatt
      721 tgcccggaga acctgtaaac acttcggcaa cgtggaacgg ttgggacagg aagcgttgga
      781 ttttacgcgc acgcattacg gtcagtttgt cttcgtcaga caattcgtcc atacccaaga
      841 tggcgatgat gtcgcgcaat tctttgtatt tctgcagggt ggactgtacg ccgcgcgcca
      901 cgtcgtagtg ctcttgaccc aagaccatcg gatccagttg gcgcgaagtg gaatcaagcg
      961 gatcgactgc cgggtaaata cccaaagagg caatatcgcg gctcaatacg acggttgcgt
     1021 ccaagtgggc gaacgttgtt gccggagacg ggtcggtcaa gtcgtccgca ggtacatata
     1081 cggcttggat agaagtaata gaaccggttt gggtagaggt aatacgctcc tgcaaacgac
     1141 ccatttcttc tgccaatgtc ggttggtagc ccactgcaga cggcatacgg cccaacaatg
     1201 cggatacttc ggtaccagcc agagtgtaac ggtagatgtt gtccacgaag aacaatacgt
     1261 cgcggccttt gccgttttcg tctttttcgt cacggaagta ttccgccatg gtcaaaccgg
     1321 tcaatgcgac gcgcaaacgg ttgcccggag gttcgttcat ctgaccgtaa accattgcca
     1381 ctttatccaa tacgttggaa tctttcatct cgtggtagaa gtcgttacct tcgcgggtac
     1441 gctcacccac gcctgcgaac acggacaagc cgctgtgcgc tttggcgatg ttgttgatca
     1501 attccatcat gttcacggtt ttacccacac cggcaccgcc gaacagacct actttaccgc
     1561 ctttggcaaa cggacacagc aagtcaatca ctttaatgcc cgtttcgagc aattcggttg
     1621 tggaagacag ttcgtcaaac ttaggagcgg cttggtggat ggcacggctc ttgtcggtat
     1681 cgattggacc tgcctcgtca acaggcgtac ccaatacgtc gacaatacga cccaatgtac
     1741 ctttacctac cggcacagta atgggcgcac cggtattgct cacagtcatg ccgcgtttca
     1801 aaccgtccga gctgcccatc gcaatggtac ggactacgcc gtcgcccaaa agctgttgga
     1861 cttccaaagt cagaccgttt tcgtctaatt tcaaagcgtc gtaaacgcgc ggaatcatgt
     1921 cgcgtggaaa ttccacgtca acaaccgcac cgataatttg tacgattttg ccttggctca
     1981 ttatcgtatc ctaatttctg tacaggattc agacggcatc agacagccgc cgcacctgct
     2041 acaatttctg acaattccgt ggtaatcgca gcttgacgcg atttgttata taccaaacgc
     2101 aactctttga tggcattgcc tgcattgtct gttgcagctt tcatggcaac catgcgggct
     2161 gcctgttcgg atgccatatt gtcgctcaac gcctgataaa ccacagactc taaatagcgg
     2221 cgaaccagat attccaacac tgcaagtgca gtcggttcgt agcggtattc ccagctgaac
     2281 ggtgatttgg gagctgaatc gccaatcacg ttctcaccga taggcagcaa tacttccatt
     2341 ctcggttctt gacgcatggt attgacaaaa cccgaataca ccagatggat tctgtcaatt
     2401 tcatgtttct cataccgttg gaagagttct gtcaaaggtc cgagcagcat ttccattttt
     2461 ggggtatcgc ccaaatttac ggcactggca accacattca gaccaatgct ctgacacgcc
     2521 atcagacctt tactgccaag gcatacgatt tcctcttcaa taccttgatt ctgatactct
     2581 tgaacttgtg ccaaaaactt tttcaacacg ttggcgttca aaccgccaca caaaccctta
     2641 tcagacgtaa tcaaaataaa accgacacgt ctgatttccc gatgagattc cagtaacgga
     2701 ataccatgat cggtattggt ttgcgcaaga tggctcatca ccatacgcac tttttcggca
     2761 tacggacgcg ccaaacgcat ccgttcctga gtcttccgca ttttagaggt tgacaccatc
     2821 tgcatcgctt tagtgatctt ttgggtattc tgaacactgc ggattttggt gagaatctct
     2881 tttcctactg ccatttcaga ctcctttcac ttcaagcctt atgcctgata ggcgtaagaa
     2941 gatttgaagg atttcatggc tgcttcaagc gttttctcgc tctcgtcgga cattgcacct
     3001 gaagcattga cggcttccaa aacttccgga tgttgggtac ggacaaagct caaaaattca
     3061 gattcaaaag ccagagcttt ggcaaccgga acatcagaat acgaaccgtt gttgattgcc
     3121 caaagggtca aagccatttc agccgtattc aacgtactga actgtttctg tttcatcagt
     3181 tcggttacga cttcgccatg ctccaattgt ttgcgcgtag cttcatccaa atcggatgca
     3241 aattgcgaga acgccgccaa ttcacgatat tgtgccaacg ccaaacggat accgccaccc
     3301 agctttttaa tcactttggt ttgtgcagca ccgcctacgc gggatacgga aataccggca
     3361 ttgattgcag gacggatacc ggcgttgaag aggtcggttt ccaagaaaat ctgaccgtcg
     3421 gtaatcgaaa tgacgttagt cggaacgaaa gcagatacgt cgcccgcttg ggtttcgata
     3481 atcggcaaag cagtcagaga accggttttg ccttttactt cgccgttggt caatttctcc
     3541 acttcgtgtt cattgacacg tgctgcacgt tccaacagac gggagtgcag gtagaacaca
     3601 tcgccgggat aggcttcgcg gccgggcgga cggcgcaaaa gcagggaaat ttgacggtaa
     3661 gccacagcct gtttggacaa atcgtcataa acaatcaagg catcttcgcc gcggtcgcgg
     3721 aagaattcac ccatcgtaca accggagtaa ggtgcgatat attgcaatgc cgccgcttca
     3781 gatgcagttg cagcaaccac aatcgtgtgt tccatcgcgc catgctcttc caatttgcgg
     3841 accacgttgg caatagaaga tgctttttga ccgatagcga catagataca gataacaccc
     3901 gtaccttttt ggttgacgat ggcatccagc gctacggccg ttttaccggt ttgacggtcg
     3961 ccaataatca actcacgctg accgcgaccg acaggaacca tagagtcaat cgccttcaga
     4021 ccggtttgca tcggctggtc aaccgatttg cgcgcaatca cgcccggtgc gattttttcg
     4081 ataggggcgg tcaaagttgt attaatcggg cctttgccgt cgataggccg acccaatgca
     4141 tcaacgacgc gtccgaccag ttcgcgtccg accggcactt ccaagatacg accggtacag
     4201 gtaaccgtgt cgccttcttt aatatgttcg tactcgccca acactacggc gccgacggag
     4261 tcgcgctcca ggttcatcgc caagccgaaa gtgttacccg ggaattcgag catctcacct
     4321 tgcattgcat ctgacaaacc atggatgcga acgataccgt cagttaccga aatcaccgta
     4381 ccacgggtac gcacttcggc atttacagac agattttcga tcttggcttt aatcaaatcg
     4441 ctaatttcag caggattaag ctgcatgaaa actctcctaa ttcgtcatag tcgtgtacaa
     4501 ggcactcaat ttgccttgta cagacaaatc caaaacctga tcacccactt caacttttat
     4561 gccgccaatc agctccggtt cgatttcgac agagattttc agctcgctgt cgaaacgctt
     4621 attcagcatt tgcgccaact cgccgacctg tttgtcggtc aacggatagg cactgtaaat
     4681 gacggcagat ttgatatggt tgaatgataa ggtcaagtct tgatattgag catatacttc
     4741 cggcaatatc gacaaacgtt tctgcccggc caagacgata acaaagtttt tcaactcctt
     4801 gtctttcaaa ccgaccaaat cgatgaggat atctgctttt tctgaagcat tcgtttcagg
     4861 acggtcaatc aatgaagcca ccttcccttc ctgaacaacc gccgcaagtt tttccagtcc
     4921 gcccaaccaa gactcaattt ggcttttttc ctgagccaga ccgaacaatg cctttgcata
     4981 aggtctggca atcgttgcga actctgccat aagattacag ctcctgtttc agggtatcga
     5041 gcagttttgc gtgtttggaa gcatcgactt cgctgcgcaa aatagattcg gcacctttga
     5101 cagccaacac ggcaacctgc tcgcgcaggg attcgcgtgc gcggaacaat tcctgctcca
     5161 catcggcctt tgcctgagct gcaatgcgcg ccgcctcgga agaagcctgt tctttggctt
     5221 cttcgacaat tttggcggca cgtttttcgg cgttggcaac catttcggaa acctgattac
     5281 gcccttctgc caagagttct gcaacctttt tttcagcctg ctcgaaatcg cttttaccac
     5341 gctcggcagc tgccaagcct tcggcgactt ttgcggcacg ctcatccaaa gcttttgcaa
     5401 tcggcggcca cacgaatttc atggtaaacc ataccaaacc gaaaaagacg atgatttgag
     5461 cgaataatgt tgcattgata ttcacgttac ttaaccttcg tattggggtt aatcaaacag
     5521 gctgcgcctg tacggaacgg acgaatccgt cctgattatg cacctgcaaa cgggttaacg
     5581 aaggcgaaca gcagtgcaat ggcgacacca atcaagaatg cggcatcaat caaaccggca
     5641 atcaggaaca gtttggtttg cagcggaccg atcagttcgg gctgacgggc agaagactcc
     5701 aaatatttag aaccgaccat tgcgataccg atagaggcac ccagtgcgcc caatgcaacg
     5761 atcaaaccac atgcgatagc aatcaaaccc attttaaact ccttaaagaa acaaaggtta
     5821 aactacaaaa acaaactact taggaaaatc agtgcgcatc atgtgcctgt ccgatataga
     5881 cgaacgccaa cgccatgaaa ataaacgcct gcagggtaat caccaaaata tggaaaatcg
     5941 cccatgccaa accggcaata atgtggaata caaacagaat cggatccatg acttcgacgc
     6001 tgccgaaagc cgcccaagca ccgccaagca aggctatcaa caagaatacc aactcgcctg
     6061 catacatatt gccgaacaac cgcataccgt gggatacggt tttagaaaga aactcgacca
     6121 aattcagcag gaagttcgca ggtgcgagtt ttgcaccgaa cggcgcgctg aacaactcgt
     6181 gaaaccatcc gcccaatcct ttgattttga tgttgtaata gatacaaatc agcaacacgc
     6241 cgacagcgag tgccaaagtg gtgttcaaat cggcagtcgg tacgacgcgc aacagggcgt
     6301 gatggttgcc ggtaatgccc tgccatacca tcggcagcaa atcgaccggc agcatatcca
     6361 tcgcgttcat caggaaaatc cagacaaaca gcgtcagacc caacggcgcg acggcttttc
     6421 tagacttttc gttgtgaatg atgctcttac acatatcgtc cacaaactca aacaagattt
     6481 ccacggcggc ctggaaacgt ccgggaacgc ctgccgtcgc tttttttgca ccgcgccaca
     6541 acagaaagct gccgattacg cccaacagga cggcaaaaaa gacggcatca aggttaataa
     6601 acgaaaaatc agcaatgttt ttcagtccct gaccctgagt aacatccgac aaactggtca
     6661 agctctgcaa gtggtgcttg atgtagtcgg cagcggtaat ggtttcacct gccataatct
     6721 ttcactctca acaatactaa aaaaaccaaa tggctgacac cgagcagccc catcagaaac
     6781 ggggcgaaca ccagcgattg atgccataca gcaaatacgg cgagcatgga caacagcgac
     6841 agcactactt ttaaaatctc tccgaagacg aacatcctgc tttgcaggaa ggggtttccc
     6901 ctgaaaagtt ttaaaagtaa aactgcaaca aacgtgggaa gcaggtagga caaaccgcca
     6961 ccgaccgccg aaaggaatcc ggcaaaaccc catacggcaa aggcaactgc ggcgcatatg
     7021 gacaatacgg cggattgtag gatgataatc tgcttcataa agggaatgtt tccgcctcga
     7081 atttgaggcg cggctaatat aatttagaag ccttattacg tcaagcaata tgttaatctt
     7141 tgtgaaacaa cgtatcccaa tccgccgcgc tcgccgcctg aataacggcg acaggtgtca
     7201 ttctaacaca cattacatat aattacagga tattaaggag tttgtccgca atttctttac
     7261 acttttaatg ttcttacgtg atttgtttgc tttacgtgga aataataaaa aatcaacgcg
     7321 aaattgtagc agtttatcgg tcggattgtc ggcagtttgg ggaatttgct caataaataa
     7381 aaggtcgtct gaaaatattt tca
//
LOCUS       DAWKST010000102         7365 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.102, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000102 DAWKST010000000
VERSION     DAWKST010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7365
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.102"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(205..2808)
                     /gene="pepN"
                     /locus_tag="WJL87_001440"
     CDS             complement(205..2808)
                     /gene="pepN"
                     /locus_tag="WJL87_001440"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ7799861.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     IGEPLVLDGSAKLLSVKINGAAADYMLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GECSDDRHWVKWEDPFAKPSYLFALVAGDLAVTEDYFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNVFELTIKQTVPPTPDMADKQPMMIPVKIGLLNRNGEAAAFDYQGKRATEAVLLLT
                     EAEQIFLLEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIELPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGA
                     ENIDPLRYHQAREALLDTLAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALIGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(2940..3734)
                     /locus_tag="WJL87_001441"
     CDS             complement(2940..3734)
                     /locus_tag="WJL87_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ7799862.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRMRNYLSAQVVEKPHWEEFVRAWE"
     gene            complement(4484..5353)
                     /locus_tag="WJL87_001442"
     CDS             complement(4484..5353)
                     /locus_tag="WJL87_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEZ7799863.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKLADLTGLLAYLLVKPRRRIGEIN
                     LAKCFPEWSEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAKRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREEDNTVTLHFYPAWESFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(5404..5940)
                     /gene="ruvC"
                     /locus_tag="WJL87_001443"
     CDS             complement(5404..5940)
                     /gene="ruvC"
                     /locus_tag="WJL87_001443"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ7799864.1"
                     /translation="MSATVRILGIDPGSRVTGFGVIDVRGRDHFYVASGCIKTPADAP
                     LADRIAVIVRHIGEVVAVYRPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LASQLNPSGMQVKRGRFQ"
     gene            complement(5943..6182)
                     /locus_tag="WJL87_001444"
     CDS             complement(5943..6182)
                     /locus_tag="WJL87_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ7799865.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6212..7222)
                     /gene="dusB"
                     /locus_tag="WJL87_001445"
     CDS             complement(6212..7222)
                     /gene="dusB"
                     /locus_tag="WJL87_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEZ7799866.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRFCRDFGAGWAVCEMLTS
                     DPTLRNTKKTLHRSDFTNEGGIVAVQIAGSDPQQMADAARYNVSLGAQVIDINMGCPA
                     KKVCNVQAGSALMQDEPLVAAILEAVVRAAGVPVTLKTRLGWHDDHKNLPAVARIAED
                     CGIAALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGDITSPQKAAAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECSAAILNHIRAMHAFYGETAGV
                     RIARKHIGWYIGEMPDGEQARRDINCLDSAAAQYDMLAGYLERLAEKTDSWACAYRPN
                     TF"
BASE COUNT         1636 a         1955 c         1941 g         1833 t
ORIGIN      
        1 acaaaaacca gtacagcgtt gcctcgcctt accgtactat ctgtactgtc tgcggctttg
       61 tcgccttgtc ctgatttttg ttaatccact ataattaata tgactttgcg gccgttttgc
      121 cattgcgtaa taaaacgatg gggaagtgat gataaaacgt gtgtgtaact atatcagacg
      181 gcattgtttt tctgtttgac ggcctcaatc caaaattttg ccgacgattt cgcccacgtc
      241 tttcgacaat ccttcctgcg cccgaatgcg ctgcaatgct tgtttcacca agtttttgcg
      301 gtgcggctcg agcttgttgc agaggttgaa cgcctgcacc aaacgggcgg cgacctgcgg
      361 gttgaagcgg tcgatttcga tgactttgtc ggcgatgaag cggtagccgc tgccgtcttc
      421 tgcgtggaaa tgcgggacgt tgcggctgaa gctgccgatg agcgaacggg ctttgttggg
      481 gttttcgagg ctgaatttcg gatgctgcaa ggcggtttga acctgttgca gggtgtcgct
      541 gcggcggctt gagccgataa gggcgaaata tttgtccatc accagcgcgt cgtctgaaaa
      601 cttgtcggca aactgcgcca gcaggcggtt gcgcgtatcg ctttcgttgc cgttgacggc
      661 ggacaggatg ccccattcgt gggtcatgtt ttgcgccatt tcgccgtatt tttcggcaac
      721 ggtttcgatg tgcgcggggt cggcgcgcag gacgaaggcg cggcagacgt tgcgcagcgt
      781 gcgccagccg gcagcttcgg ggctgtactc gtagctttgg ttttcctgct tcgccgcctg
      841 acggttcaat tcgtgccatt tcggcaggaa gcggacggca agcgtgtcca acaaggcttc
      901 gcgcgcctga tggtagcgca gcgggtcgat gttttccgcg ccgtcccaca gctcggcttc
      961 agacggcacg cccaaaagca gggctttgaa ggcgttgtct aagaggtcgt ctgaaatgac
     1021 tttttcgacg gcggcaagca gtttttcgtg tttcggcagt tcaataccgt ctgaaagtgc
     1081 ggcaaggttg gcggcgacgg cacggcggta gagcgtttgt gcggcttccc agcgcgtgaa
     1141 ggcgtcgctg tcatgggcga gcagaagcag caggtcgtcg tcgctgtacg gatagttcag
     1201 atgcaccggc gcgctgaacc cgcgcagcag cgagggaacg acggcttcgg ttacgccttc
     1261 gagcaggaag atctgttcgg cttcggtcag cagcaatacg gcttcggtcg cgcgtttgcc
     1321 ctgataatcg aatgccgccg cttcgccgtt gcggttcagc agcccgattt tgacgggaat
     1381 catcatcggc tgcttgtccg ccatatcggg cgtaggcggc acggtttgtt taatggtcaa
     1441 ctcgaacaca ttgtttttca gacgaccttc cgcttccaaa acgggcgtac ccgcctggct
     1501 gtaccacaag gcgaactggt cgagattgat gccgttcgcg tccgccatcg ccgcgcggaa
     1561 atcgtcgcag gtaacggcct ggccgtcgtg gcgttggaaa tagagcttca tgcctttttg
     1621 gaagccctct tcgccgagca gggtgtgata catccgcacc acttccgcgc ctttttcata
     1681 aacggtcatg gtgtagaaat tgttcatctc ctcatagctg gcggggcgca ccggatgggc
     1741 ggtcggacct gcgtcttcgg ggaactggtg ctggcgcaaa aggcggatgt tttcgatacg
     1801 gcgcacggcg cggctggcgc ggtcgccgga aaattcttgg tcgcggaaca cggtcagccc
     1861 ttctttcagc gaaagctgga accagtcgcg gcaggttacg cggttgcccg tccagttgtg
     1921 aaaatactcg tgtccgacca cggattcaat gccttcgaaa tcggtatcag tggcggtgcg
     1981 gctgtcggca aggacgaact tggtgttaaa aatgttcaaa cccttgtttt ccatcgcacc
     2041 catattgaaa tcgcccacgg cgacgaccat gaaaatatcc aagtcgtatt ccaaaccgaa
     2101 gcgcgtttcg tcccacttca tcgcgttttt cagcgattcc acggcaaagc cgactttggg
     2161 tttgtccgct tcggtggtgt aaaactcgat tttgacgttt ctgccgctca tagtggtgaa
     2221 atagtcttct gtgaccgcca aatcgcccgc gaccaaagca aacagatagc tcggtttggc
     2281 aaacgggtct tcccatttca cccaatggcg gtcgtctgaa cactcgccgc cgtcgatttt
     2341 gttgccgttg gagagcaaaa cgggatagcg ttttttgtcc gcaacgatgg tggtcgtgaa
     2401 cttggacatc acatccggac ggtcgatgta gaacgtgatt ttgcggaagc cctccggctc
     2461 gcactgggta aatagattgc cgccggaagc atacagcccc atcagcgatt tgttttccgc
     2521 cggcaggatt tcggtttcca cttcgacggt gaagcgttcg gacggcacgt ctgcaatcgt
     2581 cagcgtttcg ccttccaaca tataatccgc cgccgccccg ttgattttga cggacaaaag
     2641 tttcgccgaa ccgtccaaca ccagcggctc tcctatcctc tgtggctcga ccgtcaaacg
     2701 ggatttcacg acggtttgcg gttcggcaat atcgaaatgc aggtcggttt cgagaatgcg
     2761 gtaggcaggc gtttggtaat ctttcagata acggacggtt ttgctcattt ttttctttca
     2821 atattgtttg gtttgactgg aaaaggcttc agacggcatg ggcgcatccc gcgtatgccg
     2881 tctgaagccg caacggcggc acgggcgcgc cgccggacaa ccggtttgaa tttcaatctt
     2941 tattcccacg cgcggacaaa ctcttcccaa tgcggctttt ccacgacttg tgcggacagg
     3001 taattccgca tccgcttgat ttccatttcg tattcatccg catcaaacct tccgctgacc
     3061 aaacagaaac gcaggtacat cagataggta tttaccgcat cggtttcgca ataattgcgg
     3121 atttccttca gcctgcccgc gtggaacgcc tcccagacct tgctgccgtc catacccaat
     3181 ttacccggaa aaccgcacag tttcgccata tcgtccagcg gcacattcgc cctcggctgg
     3241 taaagcgcga gcaaatccat caaatcgcag tggcgttggt gataacggct gatgtagttg
     3301 ttccacttga aatcgcggct gtcgccgaaa tcgccgtcgc ccatatccca atagcgcgcg
     3361 gcgttgatgc cgtatatcag ggagcggtaa tgcagtacgg gcagatcgaa accgccgccg
     3421 ttccaactga ccagttgcgg cgtatgtttt tcaatcaatt cgaaaaattt agcaatgacc
     3481 acttcctcgc cgtcatccat ctcaccgatg gtgccgacat gtactttatc ctgcccccaa
     3541 cgcatgcagc acgaaatcgc cacaacctga tgaagatgat gctgcataaa atcgccgccc
     3601 gtctgagcac ggcgtttttg ctgggcaaac agcaccactt cgtcgtcggg cagcgaggac
     3661 ggcagctcgt acaatgttcg gataccctgg acatcaggta cggtttcaat atcgaaagcc
     3721 aaaatcgtat tcatggcagt accttgcatt caaaaacaga cttgcgccta ttgtgtcatt
     3781 aaaaggccga taaaaaaaga ggcaaccccc cacaggattg ccccaatacc tcaaatcaga
     3841 gatttacgct tcacaaacaa tacaggcttt cgcctgcggc tttgcccgcg tagctcaact
     3901 ctacgccggc aaactttcgt ttcaccgttt ccgatgaaac cccgaccaat cgcaagactg
     3961 accggaaaat cctttcagac ggcatttcct gcctgtcgtg taattccatg tagcgaaatg
     4021 tacgccattt tctacgcttt gccaagcatt ttttacaata taaatgtcaa aacattaatt
     4081 ttataaaatt gctgaaaata ttaaatatat ggatttttat tttttatatt tcaataaata
     4141 taaatttaat tttgatttat atttaaattt aagcataaaa tgtcaaatat taaaggaaac
     4201 atgaaaggca tatattaaat atttatttat aacgctatgt ttttaaagaa aattaatttt
     4261 aatatattaa ctagattgtc tgcatatatt cataggtttg cggtatttct tccaaaacct
     4321 gcttcgaatt tcccgaccaa gtcttaaaaa tattgttttt gagatactta aatagcagcg
     4381 attatcaaat gaaatctgtt catataatct gccattttgc atttaaaaaa acaatcagga
     4441 gtttcgactc gaaacgcctg atatgttttg taattttacg tagtcagtaa aaatcggggc
     4501 tgccttccgg acgggtttta aaacgcttgt gcagccaaaa atactgctcg ggatgttcgc
     4561 gcacgcgttc ctcaataaag cggttcatgc gctgcgcgtc ggcctgcgca tcttcactcg
     4621 gaaaggattc ccaagcaggg tagaaatgca atgtaaccgt attgtcctcc tccctgacgg
     4681 gaatggcggg tatcactttt gcatttgcaa gcgcggcaat gcggctcaat ccggtaatcg
     4741 ttgccgtctg aataccgaaa aaatccacaa aaaccgaatc gttgcgtccg aaatcctgat
     4801 cgggcagata cagaaacggc gcgctgcttt tgcggaactg tttgacgagg gcgcgcagcc
     4861 cttcagtgcg cccgataagg aagacgttgt gatagcggtt tcggcctttc aaaatctgtt
     4921 cgtccaatat cttgtttttt tggtgggaat acatactgat cagcgggaca tcctgattaa
     4981 gcgcgtacac cgccatttca aaagcggtga agtgcggata caggatgatg actttttccc
     5041 ccgccgccag cgcgtcgtcc aaataatgct tattgcggta acgcaccagc gatttcaggc
     5101 gtttggcagg cgcgtaccaa tataagccgt attcgagcat cagtttcgcc atatgtttga
     5161 aatgctgttt caacacggtt ttacgctttt cctcactcca ttcgggaaaa cattttgcca
     5221 aattgatttc gccgatacgg cggcggggtt tgaccaaaag gtaggcgagc aagcctgtca
     5281 gatcggcaag tttgtgcagc agcgcaaacg gcagaaactg caaaacatac agtacaaaaa
     5341 atataaattt catctcgata cacattttct tttcagacgg caaaatacaa atgccgtctg
     5401 aaactattgg aaccttccac gcttgacctg catccccgac gggttgagct gggacgcaag
     5461 cccgtggttg cgtaaggcgt gggtcagcgc gacggcaaga ccgtccgccg catccgcctg
     5521 cggtgttccc gaaagcccca gcatctgcac caccatatgc tgcacctgtt cttttgccgc
     5581 cttacccttg ccgactaccg cctgtttgac ctgcaaggcg gtgtattccg aaacgggcag
     5641 cttatggctg accaatgccg ccaatgccgc gcccctggcc tgacccaaca tcagtgtcga
     5701 tgcaggattg acgttgacga aaacctgctc cactgccgcc tgttgcggcc ggtaaacagc
     5761 aacgacttcg ccgatatgcc gcacaatcac ggcaatcctg tctgccagag gcgcatcggc
     5821 gggcgttttg atgcagccgg aggcaacata aaaatgatcg cgccccctga catcgatgac
     5881 accgaaaccc gttacgcggc tgcccgggtc gatgcccaaa atacggacgg ttgccgacat
     5941 attcacagca aaccgtgttg aatcagtttc ttacgcaagg tattgcggtt cagccccagc
     6001 atcacggagg ctttggactg gttgccgccg cattgttcca tcacgcacac cagcagcggt
     6061 ttttccacct gatgcaagac catatcgtac acgccgcaag gttcggtacc gttcaggtct
     6121 ttgaaatatt gttctaaatt ttgtctgatg cattgagaaa tatcgggaag ggtatggggc
     6181 atgattgcac tttcaaagga taatcaagtg ttcagaaggt atttgggcgg taggcgcacg
     6241 cccaactgtc ggttttttcg gcaagtcttt caagataacc tgcaagcatg tcgtattgcg
     6301 ctgccgcact gtccaagcag ttgatgtcgc ggcgcgcctg ttcgccgtcg ggcatttcgc
     6361 cgatgtacca acctatgtgt ttgcgtgcga tgcgcacacc ggcggtctca ccataaaacg
     6421 cgtgcatggc gcggatgtgg ttcaaaatgg cggcactgca ttctgccaaa ctcaaggcag
     6481 gcggtaaaac accgtgttcg gcgtaatgtt tcaaatcgcg gaagaaccac ggtctgcctt
     6541 gcgcgccgcg ccctatcatg atgccgtcag cggcggtttg tttgaggacg gcggcggctt
     6601 tttgcggcga agtgatgtcg ccgttgaccc agaccgggat gttcagacgg catttggttt
     6661 cggcgatgag ttcgtaacgc gcctcgcctt tgtacatttg cgtgcgcgtg cgtccgtgga
     6721 cggcaagggc ggcgatgccg caatcttcgg cgatgcgggc gacggcaggc aggtttttgt
     6781 ggtcgtcgtg ccagcccaaa cgggttttga gggtaacggg tacgcctgcc gcacggacga
     6841 ctgcttccaa aatggcggca accagcggct cgtcctgcat cagcgcgcta ccggcttgga
     6901 cgttgcacac tttcttggcg ggacagccca tgttgatgtc gataacctgc gccccgaggc
     6961 tgacgttgta acgcgcggca tccgccatct gctgcggatc gctgccggca atctgcacgg
     7021 caacgatgcc gccttcattg gtaaaatcac tgcggtgcag ggtctttttg gtatttctaa
     7081 gcgtcgggtc gctggtcagc atttcgcaca ccgcccaacc cgcgccgaaa tcgcggcaga
     7141 agcggcggaa gggtttgtcg gtaatgcccg ccatcggcgc aagtgcgatg gggttgtcga
     7201 taaaatagcc gccgatgtgc ataatgggcc cgcgtttcaa aaaagtacgc cattgtacat
     7261 tttttaagca ggatttccaa tctccggacg cgcccgcgat tggggcagac accgttttat
     7321 ggcataatcc gcacacagat tccctgcccc gccactcaca ggagg
//
LOCUS       DAWKST010000103         7264 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.103, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000103 DAWKST010000000
VERSION     DAWKST010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.103"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            46..990
                     /gene="tehA"
                     /locus_tag="WJL87_001446"
     CDS             46..990
                     /gene="tehA"
                     /locus_tag="WJL87_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HEZ7799867.1"
                     /translation="MSASKKFPIPLSYFSIALGLFALGLSWRYGASVGLLPALAAESL
                     LAAASVVWLLLVAAYLIKMFAYRNDFLSDLRDLVQCCFISAIPITAMLEGLALKPYQA
                     GAAAVLIYVGVAGQLAFSMYRAAGLWRGLHSLEATTPIIYLPTVATNFVSASSLAALG
                     HHDYAALFFGAGMFSWLSLEASILGRLRTAAPVGTAARGVVGIQLAPAFVGCGAYFAV
                     GGKVDGFALALIGYGCLQLLFLLRLTRWFWEGGFTMSFWGFSFGFAAMAGCGLHLAAS
                     GVLSGLGLTLATAGSAGVALLLVGTLHRIATGRFLVRG"
     gene            complement(1064..1747)
                     /locus_tag="WJL87_001447"
     CDS             complement(1064..1747)
                     /locus_tag="WJL87_001447"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ7799868.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGQLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDKDIMGLEIPTGQPLVYKLDDN
                     LKVLEKFYL"
     gene            2044..4347
                     /gene="parC"
                     /locus_tag="WJL87_001448"
     CDS             2044..4347
                     /gene="parC"
                     /locus_tag="WJL87_001448"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ7799869.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSSK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLSDENEKRKLIIKEIQADMKQYGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTTFKEGDRLKQTLEGRTV
                     LPVVILDSSGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATNQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEPEILIESEGRRGAAHKDRIPI
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            4367..5787
                     /locus_tag="WJL87_001449"
                     /pseudo
     CDS             4367..5787
                     /locus_tag="WJL87_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            5839..6306
                     /locus_tag="WJL87_001450"
     CDS             5839..6306
                     /locus_tag="WJL87_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEZ7799870.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDSSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            6410..7162
                     /locus_tag="WJL87_001451"
     CDS             6410..7162
                     /locus_tag="WJL87_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7799871.1"
                     /translation="MTDILIDNTATEAVRALIQAFSLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQDTAARITLHFGNAAVQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
BASE COUNT         1624 a         2195 c         1902 g         1543 t
ORIGIN      
        1 cactatatct gtgcgttacg acggcatgcc gtctgaaggg tgtttatgtc tgcatctaag
       61 aaatttccga ttcctttgag ctatttcagc atcgcgctgg gcttgtttgc cttggggctg
      121 tcgtggcgtt acggcgcgtc tgtcgggctg ctccccgcct tggccgccga atcgctgctt
      181 gcggcggctt cggttgtctg gctcttgctg gtggcggcat acctgatcaa aatgtttgcg
      241 taccgaaacg attttttgtc tgatttgcgc gacttggtgc aatgctgctt catcagcgcg
      301 attccgatta ccgctatgct ggagggactc gcgctgaagc cctatcaggc aggcgcggcg
      361 gcagtcctga tttatgtcgg cgttgccgga cagttggcgt tttcgatgta tcgggcggcc
      421 ggtctgtggc gcggcctgca ttccttggag gcgacgacgc cgattattta tctgcctacg
      481 gttgcgacaa actttgtcag cgcgtcatct ctggcggcgt tggggcatca tgattatgca
      541 gctttgtttt tcggcgcggg tatgttttcc tggctgagct tggaagcctc catcttgggc
      601 aggctgcgca cggcggcacc ggtcggcacg gcggcgcgtg gcgtggtcgg catccagctt
      661 gcgcccgcct ttgtcggctg cggcgcgtat tttgccgtcg gcggtaaagt cgacggtttt
      721 gcgttggcat taatcggcta cggctgcctg cagcttttgt tcttgctacg cctgacccgc
      781 tggttttggg aaggtggttt tacgatgagc ttttggggat tttcattcgg tttcgcggca
      841 atggcaggat gcggtctgca tctggcggct tctggcgtat tgtcgggctt ggggctgacg
      901 cttgccaccg ccggatcggc aggcgtggcg ctgctgcttg tcggtacgct gcaccggata
      961 gcgacggggc gtttcttggt acgcggctga tgcgttttgc cgccttgtca aaaatgccgt
     1021 ctgaacgctc aagccttcag acggcatttt ttatttcaca cccttacagg tagaattttt
     1081 cgaggacttt caaattgtcg tccaatttgt acaccaacgg ctgaccggtc gggatttcca
     1141 agcccatgat gtctttgtcg gaaatgccct cgatgtgttt tgccagcgcg cgcagggagt
     1201 tgccgtgcgc cgccaccaag acgcgtttgc cgctcaaaat cgcgggggcg atttggtctt
     1261 cccaaaacgg caatacgcgc tccagcgtta ctttcaggtt ttcgccgtcg ggtacgacat
     1321 cggcaggcag atgggcatag cggcggtctt tgtgtgcgga aaactcatcg tctttgtcca
     1381 aaagcggcgg cagggtgtcg tagctgcgcc gccagatgcg gacttgctcg tcgccgtatt
     1441 gttcggcggt ttgttttttg tccaggcctt gcagttggcc gtagtggcgt tcgttcagcc
     1501 gccatgtttt gatttgcggt acgaacagtt ggtcggattc ttccaaaacg atgttgcagg
     1561 tcttaatcgc gcgggtcagg acggatgtga aggcgatgtc gaactcatag ccgttttctt
     1621 tcagtttttt accggcggcg gcagcctcgg caagcccctg ctcgctcagc ttcacgtcgc
     1681 gccagcctgt aaacaggttt ttcgcgttcc attcgctttg tccgtggcgg ataaatacca
     1741 gttccatatc gtctccaatg tgtgaaagtg ggaaagcctt atttataaca tattttcaca
     1801 tttcccgtat ttgattcaga ttcagacacg cgcccactat ggtttgccgt tttgatttac
     1861 aataatgtcc tttgttttac attccgcata cacaatgttt caacacacag gacgacacat
     1921 aaagcaccgc cctatgtgtt gccctgattt ggaaggggtt gccccctccc aaataaagtc
     1981 tgattctacc gccccgaagg acagatgtcc aagtggcggg gtttcaaccg aaaaggaaat
     2041 acgataaata tgcaaccgca cgctccacat accgattcca atacgctgat gctcggccga
     2101 tacgccgaac gcgcctatct cgaatacgcc atgagcgtgg tcaaaggccg cgcgctgcct
     2161 gaagtttcag acggccaaaa gcccgttcag cggcgcattt tgtttgcgat gcgcgatatg
     2221 ggtttgacgg cgggggcgaa gccggtgaaa tcggcacgcg tggtcggtga gattttgggt
     2281 aaataccatc cgcacggcga cagttccgcc tatgaggcga tggtgcggat ggcgcaggat
     2341 tttaccttgc gctatccctt aatcgacggc atcggcaact tcggttcgcg cgacggcgac
     2401 ggtgcggcgg cgatgcgtta caccgaagcg cggctcacgc cgattgcgga attgctgttg
     2461 tccgaaatca atcagggaac ggtggatttc gtgccgaact acgacggcgc gtttgacgag
     2521 ccgctgcacc tgcccgcccg cctgcctatg gtcttgctca acggcgcgtc aggcatcgcg
     2581 gtgggcatgg cgaccgagat tccgtcgcac aatttgaacg aagtgacgca ggcggcgatt
     2641 gcgctgttga aaaagccgac gctggaaacc gccgacctga tgcaatatat tcctgcgccc
     2701 gattttgccg gcggcggtca aatcatcacg ccggcggacg aattgcgccg gatttatgaa
     2761 accggcaagg gcagcgtgcg cgtgcgtgcg cgttatgaaa tcgaaaaatt ggcgcgcgga
     2821 caatggcgcg tcatcgtaac cgagctaccg ccgaacgcca attcctccaa aatccttgcc
     2881 gaaatcgaag agcagaccaa cccgaaaccg aaagcgggca aaaagcagct caaccaagac
     2941 cagctcaata ccaaaaagct gatgctggat ttaatcgacc gcgtgcgcga cgagtccgac
     3001 ggcgaacatc ccgtgcgact ggtgttcgaa ccgaaatcca gccgcatcga taccgatacc
     3061 ttcatcaaca cgctgatggc gcaaacttcg ctggaaggca atgtgtccat gaacttggtg
     3121 atgatggggc tggacaaccg ccccgcgcag aaaaacctga aaacgatttt gcaggaatgg
     3181 ctggatttcc gcaccgtaac cgtaacacgc cgtctgaaat tccgtttgaa ccaagtggaa
     3241 aaacggctgc acatcctcga aggccgtctg aaagtctttc tgcacatcga cgaagtgatt
     3301 aaagtcatcc gcgaatcaga tgacccgaaa gccgatttga tggcggcgtt cgggctgacc
     3361 gaaatccagg ccgaagacat tttggaaatc cgcctgcgcc agttggcgcg tttggaaggt
     3421 ttcaaactcg aaaaagaatt gaacgagttg cgcgaggaac aaggccgtct gaatatcctt
     3481 ttgagcgacg aaaacgaaaa acgcaagctg attatcaaag aaatacaggc ggatatgaaa
     3541 caatacggcg acgcgcgacg cacgctggtg gaagaagccg gacgcgccgt gttgacgcag
     3601 accactgccg acgaacccat cacgctgatt ctgtcggaaa aaggctggat acgcagccgc
     3661 gccggacaca accttgattt gagccaaacc acgttcaaag aaggtgaccg cctcaaacaa
     3721 acccttgaag gccgcaccgt tttacctgtc gtcatcttgg attcatcggg cagaacctac
     3781 acgctcgatg ccgccgaaat ccccggcgga cgcggcgacg gcgtgccggt ttcatcctta
     3841 atcgagctgc aaaacggtgc gaaacccgtt gcgatgttga caggattgcc ggaacaacat
     3901 tatttattat caagcagcgg cggctacggc ttcatcgcca aactgggcga tatggtcggg
     3961 cgcgtgaaag cgggcaaagt ggtgatgacc gcagacagcg gcgaaaccgt tttgccgccg
     4021 gttgccgtct atgcctcctc gttcatcaac cccgactgca aaatcattgc cgccaccaat
     4081 caaaaccgcg ccctcgcctt ccccatcggc gaattgaaaa ttatggcgaa aggcaaaggg
     4141 ctgcaaatca tcggattaaa cgccggcgaa tcgatgacgc ataccgccgt ttcttccgag
     4201 ccggaaatcc tgattgaaag cgaaggcagg cgcggcgcag cgcacaaaga ccgcatcccc
     4261 atctccctgc ttgaggcaaa acgcggcaaa aaaggcaaac tattacccat atcgggcagc
     4321 ctgaaacagc tttcttcccc gaaataaacc cgactctgca catactatgg taatttccaa
     4381 tacccgcgaa cttgagaaac tcaaagaccg gattcccaat ctgatcaaca tcgcccgcat
     4441 cgccatcgtt tttccgctga tgattatgca catcctcggg ctggaaaccg gcagccgtgc
     4501 gaacctgcac gcttcgtgga cggcgtgggc attttatgtt tggctcgcca ttgcctgctg
     4561 gctgattttc ttttccatca tccatccgca ttggcaatgg cagtcgctga aaatgccgcg
     4621 tttcagcgcg gtggcggaca tcacgatgat cggcgtgctg acctacctgt tcggcggcat
     4681 cgattccggc ttcggcatcc tgattctgcc cttcgtcgtc tgctcctgcc tgctcagcta
     4741 cgggcgttac cccctgctct atgccagcta cgccgccatc ctgctgatat tcaacgccat
     4801 tgccgacggc gatatcggca aatatccgct catatcggat gcccgaaccg cctcggcaac
     4861 cttcatcctt gtcgccgcct cctatctttc cgccatcttc acctccctgt cggtcaaata
     4921 catcgaccgt gccggaaaac ttgcccatga aaaccacgcc gcctaccgcc gcatcagggg
     4981 cttgaaccaa accgtactca accgcgttca ggaagcggtc gtcgtcatca acgtcgagca
     5041 tcagaccata ctgttcaata aaaaggcaaa agacctgcta cccatgcttg aaatcggaca
     5101 gcataccgcc ctgttcgacc ctattaccgt tttatgggac aaggcctctc cgcacacttt
     5161 cgaatacgtc ctcggcacat ccggcctgaa cgccggcatc cgcgccgttc cggtcaacaa
     5221 agggccggac aagctgctta tcctctacat ccgcccgcaa agcgaaattc aggcggaagc
     5281 cctgtccgtc aaacttgccg cgctcggaca actgaccgcc aacctcgccc acgaaatccg
     5341 caacccgatg tccgccatcc gccacgccaa cgacctgctg cgcgaaaata tggaagcggg
     5401 ggcggcaaaa ccgaacgcga aaccatcggc ctgataccgt tttgggaaga attcaaacaa
     5461 gagttcctgc tcggccatcc cgatgccgcc ggctgcatcc gcttggatat gcagggcaat
     5521 cacccgaccg cctatttcga tcccgcccac ctgcggcaga ttatgtggaa tctcgccaac
     5581 aacgcgtggc ggcacagccg caaacagccc ggctcgattt ccgtcaccat ccgccccgcg
     5641 caaaaaaaca ccgtctgcat cctctttgcc gaccgcccga agtgcaggaa cacctgttcg
     5701 aaccctttta caccacggcg gaaaacggca ccggcctcgg gctgtatgtc gcccgcgaac
     5761 tggcgcacgc caatttcggc gatttgacct acctaccgga agccaaatgt ttcgaactca
     5821 cattaccgga aaaaaccaat gactgaactg caacaccccg tcctcgtcgt cgatgacgaa
     5881 accgacattc tcgacctgat ggaaatgacc ctgatgaaaa tgggcttgcg cgtccatacc
     5941 gcgtcaggcg ttgccgaagc caaaaacaag ctcgacagcc aacgctattc gctcgtcctg
     6001 accgatatgc gtatgccgga cagctcgggg ctagaagtcg tccaacacat caacagccgc
     6061 ctgctcgata cgccggttgc tgtcatcacc gccttcggca atgccgatca ggcacaggaa
     6121 gccctgcgtt gcggcgcgtt cgaccccgat accatgcaga tacaggacta tctcgaccaa
     6181 atcgaacgcg acatcatcga acaaaccctc aaacaaaccg aaggcaaccg cacgcaggcc
     6241 gccaaacgct tgggcatcag cttccgttcc atgcgctacc gtatggaacg cctcaacatc
     6301 ggctgacgac aaaacggcat ccgcaccatc tccgcccacc cgaaaaaatg ccgtctgaaa
     6361 cggcacggga aagcgggttc gccccacgcc cgaacggaca caaaacacca tgaccgacat
     6421 ccttattgac aacaccgcca ccgaagccgt ccgcgccctg atacaggcat tctcccttgt
     6481 gcccgtttcc caaccgcccg aacaaggcag ttacctcctt gccgaacacg ataccgtcag
     6541 cctcaggctt gtcggggaaa aaagcagcgt catcgtcgat tttgcctccg gcgcggcaca
     6601 ataccggcgc acaaaaggcg ggggcgaact catcgccaaa gccgtcaacc acaccgcgca
     6661 ccccaccgtt tgggacgcaa ccgcaggatt ggggcgcgac agcttcgtcc tcgcctcgct
     6721 cgggctggcc gttaccgcct tcgagcaaca tcccgccgtc gcctgcctgc tttcagacgg
     6781 catccgccgc gccctcctca atcccgaaac gcaagacacc gccgcgcgca tcaccctgca
     6841 tttcggcaac gccgcagtac aaatgcccgc acttgttcaa acacaaggca aacccgacat
     6901 cgtctatctc gaccccatgt atcccgaacg ccgcaaaagt gccgccgtta aaaaagaaat
     6961 gacctacttc caccggctcg tcggcgaagc gcaagatgaa gcggcactcc tgcataccgc
     7021 acgccaaaca gcaaaaaaac gcgtcgtcgt caaacgcccc cgcctcggcg aacaccttgc
     7081 cggacaagcc cctgcctacc aatacaccgg caaaagcacc cgcttcgacg tttacctgcc
     7141 ctacgggacg gacaagggat aacgcccata aaacaagaca ccgaaaaatt tgccgttctt
     7201 atgcaaacga gaaaccggtt tttgcgtttc gactgttttg gataaatcat cacaccttaa
     7261 agtt
//
LOCUS       DAWKST010000104         7215 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.104, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000104 DAWKST010000000
VERSION     DAWKST010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.104"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..139
                     /locus_tag="WJL87_001452"
     CDS             <1..139
                     /locus_tag="WJL87_001452"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ7799872.1"
                     /translation="LQVRIGQRFYRTTARAVIGSGGTAEIPAIADEPGAAANVATARR
                     N"
     gene            139..750
                     /locus_tag="WJL87_001453"
     CDS             139..750
                     /locus_tag="WJL87_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ7799873.1"
                     /translation="MAAPAGVATECRLTVQGGTDRESDASLLARLLKIIRRPPAGGNR
                     YDYKNWALSVDGVTSAYVYPLRRGLGTVDIAITSADGVPSEETVRRVQAYIDEMRPVT
                     AKNALVLKPTVTAVPVTVQVKLDGIDLDEAKRRIRTALKEYFDTLIPGDGLTVSQIEA
                     AISNVDGVIDRRLTAPTANRAADTVNRIEWFKAGAINVTEMPS"
     gene            747..1307
                     /locus_tag="WJL87_001454"
     CDS             747..1307
                     /locus_tag="WJL87_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ7799874.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQRRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            1318..3291
                     /locus_tag="WJL87_001455"
     CDS             1318..3291
                     /locus_tag="WJL87_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ7799875.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVTTGNQEIGGQKTFTAQTQFQSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHGANNRISYNNHDIFHKANKPRFAEDIEGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLSTAVGSSNLPVENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVRNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGMTAWFPSDQIPTDWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNSLAVGTKQEDEIKRHVHKVFSHWTNHTDTA
                     AVGYEDHNERQRSALVSTWTDGDLNDNGFLTPRPDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQNLQDKADRAHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRRDGLLEALQD"
     gene            complement(4096..4407)
                     /locus_tag="WJL87_001456"
     CDS             complement(4096..4407)
                     /locus_tag="WJL87_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HEZ7799876.1"
                     /translation="MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQS
                     SITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQRKTFDIAPLFPEPPLSSMQTGAR
                     A"
     gene            complement(4415..4693)
                     /locus_tag="WJL87_001457"
     CDS             complement(4415..4693)
                     /locus_tag="WJL87_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ7799877.1"
                     /translation="MIVSFKHKGLERFFKTGSLSGIQAGHSVKLNLLLTALNAAQTPS
                     DMAVPSWNLHPLKGSLSGHWAVKVNGNWRLTFRFNDGNAEVVDYQDYH"
     gene            4778..5383
                     /locus_tag="WJL87_001458"
     CDS             4778..5383
                     /locus_tag="WJL87_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799878.1"
                     /translation="MTIYFKNGFYDDTLGSIPEGAVAVRAEEYAALLAGQTQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAAARGVELDVLIEKVVEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            5362..5658
                     /locus_tag="WJL87_001459"
     CDS             5362..5658
                     /locus_tag="WJL87_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HEZ7799879.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKVLYTLINGVFFDRQHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            5655..5864
                     /locus_tag="WJL87_001460"
     CDS             5655..5864
                     /locus_tag="WJL87_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799880.1"
                     /translation="MNYFGMVEFLRLMAIVRPPFVFFSGTRSELPAYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(5974..7116)
                     /locus_tag="WJL87_001461"
     CDS             complement(5974..7116)
                     /locus_tag="WJL87_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEZ7799881.1"
                     /translation="MQKWQTELYSTPLWLCLTPKSTVKVLLLITVMVTLLLTEVRLNV
                     LSTFMSKGLYDSMQDLNASAFWMFAAMNAGVVLIRAFNNVVNDFLDQGLAIKWSEQLN
                     EVLTTRWLADKNYYRLQMRRHAPDNIDQRIQQDAQDFIASTIEFVRGMVNSVVTSLEF
                     AVVLWGLAGILTVFGFDIPRGIVWLVFIFVILATFIAMWIGNPLIRYNYENEKLNGDY
                     RYSLIRVRDHAESVAFYNGEQHEHGRLSDRFKAIIRNRWRIARQSVCLSGFNDMFTNG
                     IKLFPIILQAPRLFAGQIKIGDIQQTVQAFARLQNALSFFRMFYNKFTAYRARLERLY
                     GFLLSTEEQHSARQPEISEVSDGIALECKAAAAFARIGGSFCTAEA"
BASE COUNT         1819 a         2114 c         1976 g         1306 t
ORIGIN      
        1 cctgcaagtg cgtatcggcc agcgttttta ccgcactacc gcccgcgccg ttatcggcag
       61 cggcggcacg gcggaaatac cggcaatcgc cgacgagccg ggcgcggccg ccaatgtggc
      121 gacggcgagg cgcaactgat ggccgccccc gccggtgtgg ccaccgaatg ccgccttacc
      181 gtacaaggcg gcaccgaccg agaaagcgat gcctcactgc tggcgcgtct gttgaaaatc
      241 atccgccgac cgcccgcagg cggcaaccgt tacgactata aaaactgggc gttgagtgtt
      301 gacggcgtaa ccagcgcata tgtttatccg ctgcgccgcg gcttgggtac ggtggatatt
      361 gccattacct ccgccgacgg tgtgccatcg gaagaaactg tgcgccgcgt acaggcttat
      421 atcgacgaga tgcgcccggt aacggcaaaa aatgcgctgg tactcaagcc aaccgtaacg
      481 gcggtgcctg ttaccgtgca agtcaagctc gacggcatcg acttggacga ggccaagcgc
      541 cgcatacgga cggccctaaa agaatatttc gacaccctga tccccggcga cggcctgact
      601 gtgtcgcaaa tcgaggcggc tatcagcaat gtggatggtg tgatcgaccg ccgtctgact
      661 gcgccgacgg ccaaccgtgc cgccgatacg gttaaccgca tcgagtggtt taaagcgggc
      721 gcgattaatg taacggagat gccgtcatga gctatcaaga catcttgcgg ggtctgttgc
      781 cccccgtgtc gtatgcccgc aatgccccgc gtgtgcgggc gcaggcagaa atagacggcg
      841 cagcgctgga tgcggtagcg gaatcggctc aaagcgttgc cgatgccgtc gacccgcgca
      901 gcgccggcca aatgctggcc gattgggagc gcgtattagg tttggacggt acgggcaaaa
      961 accgccagcg ccgtgtgttg gccgtcatgg ccaagctaaa cgaaacaggc ggcttgagta
     1021 ttccttattt tgtgcgtttg gccgaggcgg cgggctatca aatccaaatc gacgaaccgc
     1081 agccgttccg cgccggtgtc aaccgtgcgg gcgaccgtct tgcgccgcag gaaatcatgt
     1141 gggtgtggca cgttaacgtg cgcggcggca acaaccgcat tacccgattc cgcgccggta
     1201 tctcggcggc gggcgacagg ctgaccgatt acagcgatgc cgtgatcgaa agcctgttca
     1261 accgcctcaa gcccgcccac accgctatcc gatttaccta ccgataagga ctaaaccatg
     1321 caccccatcg aaacccccga taagaccttc cacgacggcg acggcgtgtc cgaattgggc
     1381 accatcctgc ccgcgtggtg gctcaaccaa gtgcaatccg agctgctggc cgtgctgact
     1441 gcggccggta tccagccgga taagtcgcag cctaatcaat tactggcagc actgaataga
     1501 ctggctgtag ttaccaccgg caaccaagaa atcggcggcc aaaaaacctt taccgcccaa
     1561 acccaattcc aaagcggcat ccatttatcc gccaaccaga cgaactggaa cggcggccac
     1621 aaagcctaca tcggcgcgga tgccgacaac gcccacatcg tcttcggcga cgacaccctg
     1681 cgcctgcacg gcgcaaacaa ccgcatttcc tacaacaacc acgacatctt ccacaaagcc
     1741 aacaaaccgc gttttgccga agacatcgaa ggcaaaccga acacactgtc cggatacggc
     1801 atcggcaatt tcaaagtcga aaccttccgg ggcgatttga acaccctcaa aacagacggc
     1861 atctattccc tgtcgacggc ggtcggcagc tccaacctgc ccgttgaaaa caccgcctgc
     1921 catatccaag tcatcgccgg cacgaaacac ggctggtgca ggcagttggg ttatcccgcc
     1981 tacacgtccg acgtgtacga acgccaccaa acgagcagcg caaacgacaa ctggtccgca
     2041 tggaaaaaac tcaattcgga cggcatcccc gtcggcgcga tcgtatcctt tcccaaagcc
     2101 gtccgaaacc ccgcaggcta tctcaaagcc aacggcacga cctttgcaca aaacaccttc
     2161 cccgacctct accgcgcctt gggcaacagc aaccgcctgc ccgatttaag ccgtaccgac
     2221 atcggcatga ccgcgtggtt tccgtccgac caaatcccga ccgactggct ggcgtttgac
     2281 gacatccgca cgcgcgtaac cgaaaccgct tatcccgagc tgtaccgtct gctgaccgga
     2341 aaatacggca gcatccaaaa cgtcccgcag gcggaagacc gctttatccg caacgcaggc
     2401 aacagcttgg cagtcggaac gaagcaggaa gacgaaatca aacggcacgt ccacaaagta
     2461 ttttcacact ggacaaacca cacagacacg gcagccgtcg gttacgaaga ccacaacgaa
     2521 aggcagagaa gcgcgctcgt atcgacttgg acggacggcg atttaaacga caacggcttt
     2581 ttaaccccgc gcccggacag caaaatggcg acaggcggcg acgaaaaccg ccccaaagcc
     2641 ctggttttaa aactgtgcat caaagccgcc gacaccttgg gcgaagccgt gttttggata
     2701 aagtcccacg gcgaaaccat caacgccggc gcgctggacg cgggcacgct ggcgcaaaac
     2761 ctccaagaca aagcagaccg cgcccacacc cacaccgccg cccaaatcca agggctggac
     2821 gaaaaaatca gcaccgccgt tgccgcgcaa ttcacacgcc aaaccatcgg cggcgtggat
     2881 attgtcagat tccccgacgg cacaatgata cagaccggca gctacaggtt cacacgaagc
     2941 ggcggcccca tcgaaaacga agtcgtcttc cccgtcgcct ttgccgacgg caacgtcaaa
     3001 tgcttcgtat ccgaacgcca ttcgggacgc gttaccggcg atcgaaggca acacaactgg
     3061 ctgtttatcc gcgcaaaaaa ccacgccgcc gccattatta ccaactggta cgaaggcagt
     3121 tgcgactgga tggccatcgg caaagccgcc tcgggaaacg ccgccagctc cccgataggc
     3181 cccgaaatac ctgaaaccaa cgaagaaccg caaagagaga gtggaagaac atcaaccgga
     3241 ccccgaaacc gccgccgccg agacggcttg ctcgaggcac tgcaagacta gcgggctgta
     3301 gagatggctg tagagacggg ctgtagagat ggctgtagag atggctgtag agatggctgt
     3361 agagacgggc tgtagagatg gctgtagaga cgggctgtag agatggctgt agagatggct
     3421 gtagagacgg gctgtagaga tggctgtaga gacgggctgt agagacgggc tgtagagatg
     3481 gctgtagaga cgggctgtag agacgggctg tagagatggg ctgtagagat gggctgtaga
     3541 gatgggcttc agcccgccga tccaagcaat ccgaccgaaa ccggccgcgc cgccaatccc
     3601 ccgaaaccta tgccccgcca atcctgccac tcttcgtcat tcccgccgct ttcgtcattc
     3661 ccgcgaaagc gggaatccag cccctaaacg cggcaggaat ctatcggaaa aaacagaaac
     3721 ccccgccacc gtcattcccg cgcaggcggg aatccagacc ccaaacgcgg caggaatcta
     3781 tcggaaaaaa ccgacccccc gccgccgtca ttcccgcgca ggcgggaatc cagaccccaa
     3841 acgcggcagg aatctatcgg aaacggctga aaccgaacgg actggattcc cgcctgcgcg
     3901 ggaatgacgg cggcaggggt ttcgggattc ccgccttcgc gggaatgacg gaaagtggcg
     3961 ggaataacga aaggcgggaa tgaccgcgca aaaagccgct gcccccttcg gacggcaccg
     4021 gcaacaaaaa accgcacggc cgaaaccgcg cgggaaagga tagtcgggcg cgcccgataa
     4081 gcagcggccg cagggttaag cccttgcacc tgtttgcatc gaagacaaag gcggttcggg
     4141 aaataagggc gcaatatcaa aagttttgcg ttgcctgatt tgccacaaat cataaccggc
     4201 ctgcatattc agccaaaaat caggcgaagt attgagcagt tggcttaaac gaaccgccat
     4261 ctcgggcgta atcgaagatt gggcgttgac aatgcgcgat aaggcaaccc ggctgacacc
     4321 caggcgtttc gctatatcgg taatgcttgc accgttaata tactctttta aaacaaggcc
     4381 cgggtgggca ggattgtgca ttttcatatt attcctagtg ataatcctga taatcgacaa
     4441 cttcggcatt gccgtcgttg aaacgaaaag tcaaacgcca atttccgttg actttaaccg
     4501 cccaatgtcc cgacaaactg cccttgaggg gatgaagatt ccaactcggc acagccatat
     4561 ccgaaggcgt ttgcgcggca ttcagcgcgg ttagaagcag attcagtttg acggaatgcc
     4621 cggcttggat gcccgatagc gagcctgttt taaaaaagcg ttcaagccct ttgtgtttga
     4681 aagaaacaat catcatccca ccccgtctgt atcgccaaag gatacaaaac ttttatttaa
     4741 ttgtaaagcg acgatataca aaaaaggaaa ccccgaaatg accatctatt tcaaaaacgg
     4801 cttttacgac gacacattgg gcagcatccc cgaaggcgcg gttgccgtcc gcgccgaaga
     4861 atacgccgcc cttttggcag gacagacgca gggcgggcag attgccgcag attccgacgg
     4921 ccgccccgtt ttaaccccgc cgcgcccgtc cgaataccac gaatgggacg gcaagaaatg
     4981 ggaaatcggc gaagccgctg ccgccgcccg tttcgccgaa caaaaaaccg ccacggcatt
     5041 ccgcctcgcg gcaaaggcgg acgaactcaa aaacagcctc ttggcgggct atccccaagt
     5101 ggaaatcgac agcttttaca ggcaggaaaa agaagccctc gcgcggcagg cggacaacaa
     5161 cgccccgacc ccgatgctgg cgcaaatcgc cgccgcaagg ggcgtggaat tggacgtttt
     5221 gattgaaaaa gttgtcgaaa aatccgcccg cctggccgtt gccgccggcg cgattatcgg
     5281 aaagcggcag cagctcgaag acaaattgaa caccatcgaa accgcgccag gattggacgc
     5341 gctggaaaag gaaatcgaag aatggacgct aaacatcggc tgaaaaaata cgtttaccac
     5401 ctgttggtag ccatcgacca actgttcaac gccctaaccg gcggcgcggc ggacgaaacc
     5461 ctctcaagcc gcacctatcg cggcgcgcgg ctcgcccaaa agcccaaaac ccgctggaag
     5521 gttttataca ccctgatcaa cggcgtattt ttcgaccgcc aacactgccg gcaggcgtat
     5581 atcagcgaac tgaaaggcag gcagcacgac gcgcggttca accaaagccg cgccgccggg
     5641 gaaaagggga cgcgatgaac tacttcggca tggtagagtt tctgcgcctg atggcaatag
     5701 tgcggccgcc atttgtcttc ttctccggca cccgcagcga actgcctgct tatcttgacc
     5761 tcgtggcaga actgcgcctg accggatggg agcgttttgc aggcagccaa accttgactg
     5821 tgagcagcac atcaacagca attcaagcta cgacgaccac ctgatttata agttctgacc
     5881 gcaagtagcg tactactttt aaaggcataa gataatcccc gtttaacaga ccattaaacg
     5941 gggataaatt tgtgcaaaag ctaatacaat ttcctacgct tcggcggtgc aaaagctgcc
     6001 gccaattcgt gcaaaagctg ccgccgcctt acattccagt gcaatgccgt ctgaaacttc
     6061 gctaatctcg ggttgccgcg cgctgtgttg ttcttcggta ctcagcaaaa agccgtacag
     6121 acgctccaat cgggcgcggt aggcggtgaa tttgttgtag aacatccgga aaaaagatag
     6181 cgcgttttgc agtcgcgcaa aggcttggac ggtctgctgg atgtcgccga ttttgatttg
     6241 tccggcaaac aggcgcggcg cttgcaaaat aatggggaag agtttgatgc cgttggtgaa
     6301 catatcgtta aagccgctta agcagacgct ttgccgcgcg atgcgccaac ggttgcggat
     6361 aatggcttta aagcggtcgg aaagccggcc gtgttcgtgt tgttcgccgt tgtaaaacgc
     6421 tacgctttcg gcgtggtcgc gtacgcggat gagggaataa cggtagtcgc cgttgagttt
     6481 ttcgttttca tagttgtaac gaatcaaggg gttgcctatc cacatcgcga taaaggtcgc
     6541 caaaatcaca aaaataaaga cgagccaaac aatgccgcgc ggaatgtcga agccgaacac
     6601 ggtcaggatg ccagccaagc cccacaaaac aacggcaaat tccaaagagg taacgaccga
     6661 attgaccatg ccgcgtacaa attcgatggt cgaagcgatg aaatcctgcg cgtcctgttg
     6721 gatacgttgg tcgatgttgt ccggcgcgtg gcggcgcatt tgcaggcggt agtagttttt
     6781 gtcggcaagc cagcgcgtcg tcagcacttc attgagctgc tccgaccatt taatcgccaa
     6841 gccttgatcg aggaagtcgt tgacgacatt gttaaacgcc cgtatcagca ccacgcccgc
     6901 gttcatcgcg gcaaacatcc aaaacgcgga ggcgttcaaa tcctgcatcg agtcgtaaag
     6961 tcctttggac ataaaggtac tcaacacatt cagccgtact tcggtcaaca gcagcgtaac
     7021 cattaccgta atcagcagca agactttgac cgtgcttttc ggtgtcaggc aaagccaaag
     7081 cggtgtggaa taaagctcgg tttgccattt ctgcatggga aatttcttac ggtatcaatg
     7141 ccgtctgaaa aagacgggta cagttgattt tttgatgaag tttggggaag ttttgccggt
     7201 cagggtacat tgcgt
//
LOCUS       DAWKST010000105         7204 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.105, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000105 DAWKST010000000
VERSION     DAWKST010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.105"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(63..147)
                     /locus_tag="WJL87_001462"
     tRNA            complement(63..147)
                     /locus_tag="WJL87_001462"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Leu,seq:caa)
     gene            424..1077
                     /locus_tag="WJL87_001463"
     CDS             424..1077
                     /locus_tag="WJL87_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ7799882.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQVASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGDQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            1156..1809
                     /locus_tag="WJL87_001464"
     CDS             1156..1809
                     /locus_tag="WJL87_001464"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ7799883.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            1913..2812
                     /locus_tag="WJL87_001465"
     CDS             1913..2812
                     /locus_tag="WJL87_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ7799884.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            2809..4098
                     /gene="hisD"
                     /locus_tag="WJL87_001466"
     CDS             2809..4098
                     /gene="hisD"
                     /locus_tag="WJL87_001466"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ7799885.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            4144..5241
                     /locus_tag="WJL87_001467"
     CDS             4144..5241
                     /locus_tag="WJL87_001467"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ7799886.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            5238..6155
                     /gene="hisB"
                     /locus_tag="WJL87_001468"
     CDS             5238..6155
                     /gene="hisB"
                     /locus_tag="WJL87_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ7799887.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            6250..7119
                     /locus_tag="WJL87_001469"
     CDS             6250..7119
                     /locus_tag="WJL87_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ7799888.1"
                     /translation="MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
BASE COUNT         1880 a         2124 c         1724 g         1476 t
ORIGIN      
        1 tagttttatc tatttcatta aacagcaata gacaaaaaaa ataaccgctc taaaagcggt
       61 tgtggtgccc agggtcggac tcgaaccgac acaccttgcg gcgggggatt ttgagtcccc
      121 tgcgtctacc aatttcgcca cctgggctgg tgaagaagtc gtcattataa tggcttttga
      181 aattctgtaa accttttttt tgaaattatt ttatctgttt ttattttatt tttgatttta
      241 aatagaattt ttattatttt aatcttactg ttctttccgc tccaaagatt ctgtatgatt
      301 cggcaattcc tgccgtgcag acaacgtaaa aaaatactac attaaatctg ccaaacgcgt
      361 taagatggaa atattcaaat tccgtacgaa tcaggttttg ctatttattc ttgggagatt
      421 gtcatgtttt ccgtaccgcg ttcctttttg ccgggcgttt tcgtacttgc cgcgcttgcc
      481 gcctgcaaac ctcaagacaa cagtgcggcg caagtcgctt cttcaagtgc atccgcgtcg
      541 gctgcggaaa atgcggcaaa gccgcaaacg cgcggtacgg atatgcgtaa ggaagacatc
      601 ggcggcgatt tcacgctgac cgacggcgaa ggcaagcctt tcagcctgag cgatttgaaa
      661 ggcaaggtcg tgattctgtc tttcggcttt acgcactgtc ccgatgtctg cccgacagag
      721 cttttgacgt acagcgacac gttgaagcag ttgggcgggc aggctaagga cgtgaaagtg
      781 gtgttcgtca gcatcgatcc ggaacgcgac acgcctgaaa tcatcggcaa gtatgccaaa
      841 cagttcaatc cggactttat cggtctgacg gcaacgggcg accaaaacct gccggtcatc
      901 aagcagcaat accgcgtggt ttctgccaaa gtcaatcaaa aagacgacag cgaaaactat
      961 ttggtcgacc actcttccgg tgcgtatctc atcgacaaaa acggtgaggt tgccattttc
     1021 tcgccttacg gaagcgagcc ggaaacgatt gctgccgatg taaggaccct gctctgataa
     1081 aaccgtatgc cgtctgcacc gtcggcgcct gttcagacgg cattattgtt tcaaccgaca
     1141 aaggacatcc acaccatgca ggataatgct ttgacaatcg ccttatccaa ggggcgcatt
     1201 tttgaggaga cgctgccgct gcttgccgct gccggcattg ttccgactga agagcctgaa
     1261 aaatcgcgca agctgattat cgggacgaac catgaaaaca tccgccttgt cattgtccgc
     1321 gcaaccgatg tgccgactta tgtccgctac ggcgcggcgg acttcggcat tgcgggcaaa
     1381 gacgtgctga tcgaacacgg cggcacgggg ctttaccggc ctttggattt ggagattgcc
     1441 aagtgccgca tgatggttgc tgtgcgtaaa gggtttgatt acgaagcagc ttcgcaaccc
     1501 ggatgccgtc tgaagattgc cacgaagtat cctgaaatcg cggcatctca ttttgccggc
     1561 aagggtgtcc atgtggacat tatcaaactg tacggctcga tggaacttgc gccgctggtc
     1621 ggcttgagcg atgcgattgt ggacttggtt tcgacgggca acaccttgaa ggcaaacggc
     1681 ttggaagcag tcgaacacat cgtcgacatt tccagccgcc tggtggtcaa caaggctgct
     1741 ttgaaaacga aatacgcgct gctggagccg attattcagg cgttcggcgg cgcagtgaag
     1801 gcgaagtaag catccatttg aataaagatg cgttttcaga cgaccctatc cgttcccgcc
     1861 gacaggtcgt ctgaaaatat caccggcagt aaactgtata ggagaagtta aaatggttgc
     1921 aaaaataaaa aaattctcag attcaaccct ttccgttttg aataacggcg agcgtcggtt
     1981 ttatgtctat tgtctgaccg acctgaaaaa agacaaaatc ctctacatcg gcaaaggctg
     2041 cggtaatcgt atcttcgagc atgaatgggt tgctagtcgt tcacaagacc cagtctccgg
     2101 cgagattatc gatcggaaac tcaaagccat ctccaaatgc aagaaactcg gtcgctatat
     2161 catcagctat catctgactg aagtcgaagc actcgccgcc gaatctgcct taattcattt
     2221 tgttaaatct gtcttgggta aaaaactcaa aaataaaatt gccgggcatg gtccgggtgg
     2281 tattagcgta gaagaactag atcgccgctt tggattctct tctctcccac ttaacgagat
     2341 taaccccgac gggctgattc tcgccatcaa aatccacaat gctttcgatt tagatactga
     2401 cgaagaatta gactaccttt tcgacaacca agacgatgcc aacctcaaat cgcgtacgtt
     2461 gggcaactgg gttatcggta aagatgttgc ttcaaaagtg aaatacgtta tcggcgttca
     2521 caccggtctg caaaacgctg ttgtcagtgc atacgaagtg gacggttttg aaacaatggt
     2581 tgaggaaacc aaaaacggta gaaaacaatc ccgttaccgt ttccgcacta cctctcgtag
     2641 cgaagaggta ttagccaaac tcggtctgca acaaaaatgc ctgcccgaat tgaagtttgg
     2701 tagcggggga gaaaaagcgt atatcagacc caaaacagag acagaaactg aacaagagaa
     2761 tattcagacg acccccaatc caaaaataaa aaaggaaaaa accaaatcat gaaaaaactc
     2821 aacacccaat cgcccgattt ccaagccgga ctcaaagccc tgctggcttt tgaaaccgcg
     2881 caaaaccccg aaaccgaacg catcgtcgcc gacatttgcg ccgacgtgca aaagcgcggc
     2941 gatgcggctt tgattgaata caccaacaaa ttcgatcaga caaacgctaa aagcatcgat
     3001 gatttaatac tcacgcaagc cgatttgaac gcggcgttcg agcgcattcc gaacgacgtt
     3061 cagacggcat tgcagaccgc cgcccgccgt gtcgaaagct accaccaacg ccaaaaaatg
     3121 gaatcgtgga gctacaccga tgaagacggc acgctgttgg gacaacagat taccccgctt
     3181 gaccgcgtcg gcatttacgt ccccggcggc aaggcggcat atccgagttc cgtcatcatg
     3241 aacgccatgc ccgcccacgt cgcaggcgtg aaagaaatca tcatggtcgt gccgacaccg
     3301 aaaggcgaac gcaacgacat cgtgcttgcc gccgcatacg tcgccggcgt aaccaaagtc
     3361 ttcaccgtcg gcggcgcgca ggcggttgcc gccctcgcct acggcacgga aaccatcccc
     3421 caagtcgata aaatcaccgg tccgggcaac gccttcgtcg ccgccgccaa acgccgcgtg
     3481 ttcggcgtgg tcggcatcga catggtggcg gggccgtctg aaatcctggt catcgccgac
     3541 ggcacgacac ctgccgattg ggtggcgatg gatttgttca gccaggccga acacgacgaa
     3601 attgcccaag ccatcctcat cggcacgtcg caagcgtatc tcgacgaagt agaagccgct
     3661 atggaccgcc tgatcgaaac tatgccgcgc cgcgacatca tcgaagcctc gctcggcaac
     3721 aggggcgcga tgatactcgc caaagacttg gacgaagcct gcgaaatcgc caactacatt
     3781 tcccccgaac acttggaact gtcagtcgaa aatccgcagg aatgggcgaa aaaaatccgc
     3841 cacgccggtg cgattttcat gggacgctac accggcgaaa gcctcggcga ctactgcgcc
     3901 ggtccaaacc atgtattgcc caccagccga accgcccgct tttcctcgcc tttggggaca
     3961 tatgatttcc aaaaacgctc cagcctgatt caggtttcgg aacagggcgc gcaaaaatta
     4021 ggcgaaaccg ccagcgtgct ggcacacggc gaaagcctga ccgcccacgc ccgcgcggca
     4081 gagttccgta tgaaataatg ccgaaacggc gtacaggcat attccaacca ttaaggaaac
     4141 acgatgaaat ccgtccgctc cttcatccgc gacgacatac aagctatgtc ggcatatcag
     4201 attgccgacg ttccgcccgg ctttgccaaa ctcgattcga tggaaagtcc cgtccaccct
     4261 tttgccggac atgaaacgct gttgcaggaa tggcaggcac ggcttgccgc cgcgcccatc
     4321 catctttacc ccaatccctc cggcagcggt ttacaggaag cattacgttc ggcgttcgac
     4381 attcccgact gcgccgacat cgcgctgggc aacggctcgg acgagctgat acagttcatc
     4441 acgatgctga ccgccaaacc gggcgcggca atgttggcag ccgaacccag tttcgtcatg
     4501 taccgccaca acgccgcgct gtacggcatg gattatgtcg gcgttccact gaacggagat
     4561 ttcaccctca acctgcccgc cgtcctcgaa gccgtcagga agcaccgccc tgccctgacc
     4621 tttatcgcct accccaacaa ccccaccggc gtatgtttca cgcgtgccga aatcgaagcc
     4681 gtcatcgaag cttcagacgg catcgtcgtc gtcgacgaag cctacggcgc attcaacggc
     4741 gacagcttcc tgccgcaggc gggcagcatt cccaacctca tcgtcatgcg taccgtcagc
     4801 aaaatcggtt ttgccggact gcgtatcggc tatgcggcag gctgtcccga agtcatcggc
     4861 gaactgcaaa aaatcctgcc gccctacaat atgaaccaac tgagcctgac caccgccaaa
     4921 ctcgccctgc aacactacgg catcatctct gccaacatcg acagcctgaa aaacgaacgc
     4981 gaacggatgt tcgccgaatt gggcaaaata tgccgtctga acacctttcc aagtcaggca
     5041 aacttcatta ccatacgcgt acccgatgcc gatttgttgt ttgacacgct caaacaaaac
     5101 cgcatcttgg ttaaaaaact gcatggcgcg cacccgcttt tggaacactg cctgcgcatt
     5161 accgtaggca gccccgcaca aaacgatgcc gttctcaaca tcattcgcca actttactgc
     5221 caaccaacgg atttcctatg aatttgacta aaacacaacg ccaactgcac aactttctga
     5281 ccctcgccca agaagcaggt tcgctgtcca agctcgccaa actctgcggc taccgtaccc
     5341 ccgtcgcact ctacaaactc aaacaacgcc ttgaaaagca ggcagaagac ccagatgcac
     5401 gcggcatccg tcccagcctg atggcaaaac tcgaaaaaca caccggcaaa cccaaaggct
     5461 ggctcgacag aaaacaccgc gaacgcactg tccccgaaac cgccgcagaa agcaccggaa
     5521 ctgccgaaac ccaaattgcc gaaaccgcat ctgctgccgg ctgccgcagc gttaccgtca
     5581 accgcaatac ctgcgaaacc caaatcaccg tctccatcaa cctcgacggc agcggcaaaa
     5641 gcaggctgga taccggcgta cccttcctcg aacacatgat cgatcaaatc gcccgccacg
     5701 gcatgattga catcgacatc agctgcaaag gcgacctgca catcgacgac caccacaccg
     5761 ccgaagacat cggcatcaca ctcggacaag caatccggca ggcactcggc gacaaaaaag
     5821 gcatccgccg ttacggacat tcctacgtcc cgctcgacga agccctcagc cgcgtcgtca
     5881 tcgacctttc cggccgcccc ggactcgtgt acaacatcga atttacccgc gcactaatcg
     5941 gacgtttcga tgtcgatttg tttgaagaat ttttccacgg catcgtcaac cacagtatga
     6001 tgaccctgca catcgacaac ctcagcggca aaaacgccca ccatcaggcg gaaaccgtat
     6061 tcaaagcctt cgggcgcgcc ctgcgtatgg cagtcgaaca cgacccgcgc atggcaggac
     6121 agaccccctc gaccaaaggc acgctgaccg cataaaaaac cataccgtct gaaacacccg
     6181 caggcttttc agacggtatc ggaacagata agattacact acactacaaa cagaaaagga
     6241 gtaaacatca tgtccgcaaa cgaatacaca caaatcggct ggataggctt agggcaaatg
     6301 ggtctgccta tggtaacgcg gctcttggac ggcggcatcg aagtcggcgt atacaaccgc
     6361 tcgcccgaca aaactgcccc catctccgcc aaaggcgcaa aagtttacgg caacaccgcc
     6421 gaactcgtcc gcgactatcc cgtcattttc ctgatggttt ccgactatgc cgccgtgtgc
     6481 gacatcctga acggagtccg cgacggattg gccggcaaaa tcatcgtcaa catgagcacc
     6541 atctccccga ccgaaaacct cgccgtcaaa gcacttgtcg aagccgcagg cggacagttt
     6601 gccgaagcac ccgtttccgg atcggtcggg cccgccacca acggcacgct gctgattctg
     6661 ttcggcggca gcgaagccgt tttaaacccg ctgcaaaaaa tattttccct cgtcggcaaa
     6721 aaaaccttcc atttcggcga tgtcggcaaa ggttcgggcg cgaaactcgt cttgaactcg
     6781 ctcttgggca ttttcggcga agcgtacagc gaagcgatgc tgatggcgcg gcagttcggc
     6841 atcgataccg acaccatcgt cgaagccatc ggcggctcgg caatggactc gcccatgttc
     6901 caaaccaaaa aatccctgtg ggcaaaccgc gaattcccac ccgccttcgc cctcaaacac
     6961 gcctccaaag acctcaacct cgccgtcaaa gagcttgaac aggcaggcaa caccctgccc
     7021 gccgtcgaaa ccgttgctgc cagctaccgc aaagcagtcg aagccggcta cggcgaacag
     7081 gacgtttccg gcgtttacct gaaattggca gaacactgat tgccttttcc aaacacaatg
     7141 ccgtctgaac atatttcaga cggcattttt atcaccccac gcttaaaatc agtcccgatt
     7201 atga
//
LOCUS       DAWKST010000106         7087 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.106, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000106 DAWKST010000000
VERSION     DAWKST010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.106"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(66..425)
                     /gene="rnpB"
                     /locus_tag="WJL87_001470"
     ncRNA           complement(66..425)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WJL87_001470"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(504..1607)
                     /gene="prfB"
                     /locus_tag="WJL87_001471"
     CDS             complement(504..1607)
                     /gene="prfB"
                     /locus_tag="WJL87_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ7799889.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1710..2603)
                     /locus_tag="WJL87_001472"
     CDS             complement(1710..2603)
                     /locus_tag="WJL87_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ7799890.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIQL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2945..3394
                     /locus_tag="WJL87_001473"
     CDS             2945..3394
                     /locus_tag="WJL87_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ7799891.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3468..3776
                     /locus_tag="WJL87_001474"
     CDS             3468..3776
                     /locus_tag="WJL87_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799892.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3892..4734
                     /locus_tag="WJL87_001475"
     CDS             3892..4734
                     /locus_tag="WJL87_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ7799893.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            4724..5470
                     /locus_tag="WJL87_001476"
     CDS             4724..5470
                     /locus_tag="WJL87_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ7799894.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLLDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5492..6079
                     /locus_tag="WJL87_001477"
     CDS             5492..6079
                     /locus_tag="WJL87_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ7799895.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6085..6264
                     /locus_tag="WJL87_001478"
     CDS             6085..6264
                     /locus_tag="WJL87_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ7799896.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            6496..6936
                     /locus_tag="WJL87_001479"
     CDS             6496..6936
                     /locus_tag="WJL87_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799897.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1806 a         1885 c         1615 g         1781 t
ORIGIN      
        1 caacctaaat aaaaatgccg tctgaaccat atttcaggtt tcagacggca tttgcgtgtc
       61 ggatgcacac cggacaggcg gtaagccggg ttctgtctcg gacagtcatt cctctaggca
      121 taccgttacc agtatgctca agcaacctac ccgaacgctc ggcgggcagc gtcattgcgt
      181 tctgtttggt cttgctccga atggggtttg gcctgccgca tattgttacc aaatgcgcgg
      241 tgcgccctta ccgcaccttt tcacccttgc ctgtgctgcc aaagcagcca tcggcggttt
      301 tgctttctgt tccactttcc gtcgcgttac cgcgcccggc cgttaaccgg cattctaccc
      361 tgcggagccc ggactttcct ccccgtatgc cttacgcgat acgcggcgac tgtctgctcg
      421 tcccgtgtgc ggcgcggatt ataacacgaa acgcaaaaat gccgtctgaa acggtacagg
      481 tttcagacgg catacagcct aaactacacg ccctgtttca ggctggcttc gataaagccg
      541 tccaaatcgc catccaatac ggctttggtg ttgccgactt cgtagcctgt acgcaagtct
      601 ttgatacgtg aggaatccaa aacatacgaa cggatttggc taccccaacc tacatcggat
      661 ttaccttctt ccaacgcctg tttctcttca ttgcgtttgc gcatttccaa ttcatacagt
      721 ttggacttca acatttccat cgcggcggct ttgttggcgt gttgcgaacg gtcgttttga
      781 cattgcacca caatccccgt cggctcgtgg gtaatacgca cggcggagtc ggttttgttg
      841 atgtgctgac cgcccgcgcc cgatgcgcga taggtgtcga tacgcaaatc ggcggggttg
      901 atttcgattt cgatggaatc atcgatttca gggtaaacga acacggaggc aaacgaagta
      961 tggcgtttgt tgttcgagtc aaacggtgaa tagcgcacca agcggtgaac gccagtttcg
     1021 gtacgcagca aaccataggc gtattcgcct tccactcgga tagtcgcgcg gttgatgccc
     1081 gcgatttcgc cgtcgtcttc ttcgaggatt tcgattttga agcctttgcg ctcggcgtag
     1141 cggctgtaca tgcggaacag catacccgcc cagtcttccg cttccgtacc gcccgcgcct
     1201 gcggtgatgt cgataaagca gttgttcggg tcggcgggct ggttgaacat ccgtttgaac
     1261 tccaaatccg ccatctgttt ttccagcccc gccacatctt cctgcacggc ggcaaaacct
     1321 tcttcgtcgt tttcttcgac ggtcatttca atcagcatgc ggttgtcttc gatgcccgaa
     1381 gcgatgttgt cgagcgtcaa cacgatgcct tcgaggattt tgcgctcttt gccgatttct
     1441 tgggcgcgtt tcgggtcgtt ccaaagttcg gggtcttcgg aaagaccgat aacttcttcc
     1501 aatcggtctt tcttaccctg ataatccata taaactcgga tgtcttcgct gcgcttttcc
     1561 aaatcattca gggtattgtt gagctggttg attacttcgg cttccatgat tcttttgttc
     1621 tttcaaaatt ttaggggcgt attgtacggg attcgggtat ttttttctat ggataaagcc
     1681 ttctagaaac acgttcggac ggcatagcgt caataacggt atgccgccag tttgcgtttg
     1741 atttcaggca atgcggcacg tgctgcctcc tcacccaact ggatggcacg ttttttctga
     1801 tcgaatccgc cgactgcacc caaatccaaa acctgcggtt tgataaccac atccgcctgc
     1861 cccaactcat tttgcaacgc ggaaacgctc attacgttca gcgtctgatc gagataagag
     1921 aagaagcctt ggctgatgtt tttgcccgga cgggcggaaa tatcgacggc aatcacgaaa
     1981 ttcgccccct gccgccgggc ggcactgacg ggcacgggct gcgacagacc gccgtcaaca
     2041 tatgtatgcc tgccgatgat aacgggttgg aacacattgg gaatggcggc ggaagcgcgc
     2101 acagcctgcc cggcattccc ttgattgaaa gcgacggcct tgccggtttc aaaatcagta
     2161 gcaacagcgg caaatttgat gggaaactgc tgaatctgcc tgccgccgac ttttcggttg
     2221 atgtaatttt gcagcttttc gcctttgata aaaccactgg tggacaaggt taaatcgact
     2281 aaatcggttt tgcctaaaat ttcggcttcc aattcgaggc ggtcgggcga catacccgat
     2341 gcaaaaaggc tgccgacaat cgaacctgcc gatgtgccgg taaccacctt cacaggaata
     2401 ccgttttctt tcaaaacctt aataatacct acatgggcaa atcctttaga tgcgccgcca
     2461 ccgagtgcca aaccgaccac tgcggcgggt ttggcggttt gcaccggctt gcgggcggca
     2521 ttatttcccg ccgtgccgca ggcggcaagc aacgcggcgg cggcgattgc caaaagcggt
     2581 ctgatttttg aaaacgttac catattttcc attcctttat atatcgcacc ccgtcaaaaa
     2641 gagggattgc ttttcttaac aacccccttt gacagccaag caaatggagg ctttgttaag
     2701 tcatcatcaa aattaatatt tctttttttc ctttacggaa attatatttg aaggcatact
     2761 atccaaggtg ggaattatct cacaacaccg ccgttatcca aatatcccgc ctttttccct
     2821 tcctttccat caaaatactt tctttttata ttcattaact tgttaaatca ttggctgccg
     2881 ggtgtcagtt tttccgacaa aatccgtcta atggggtatc aacagaacca aaacaggaac
     2941 acttatgaaa atcggaacaa cttggcagac ggcatccgct atgctggttt tgcgtctgtt
     3001 tgccgcatat gaatttttgg aatcgggttt gcaaaaatgg aacggggaga attggttttc
     3061 tgaaatcaac gatcaattcc cattcccgtt caacttgctg ccggacgcgt taaactggaa
     3121 tctcgctatg tatgcggagc ttttactgcc cgtactgttg cttttgggtt tggcaacgcg
     3181 tctgtcggca ttagggctga tggtcgttac cgccgtcgct tgggctgcgg ttcacgccgg
     3241 ttcgggttac aatgtctgcg acaacggtta taaaatggct ttaatttata tcgtggtatt
     3301 aatcccgctg cttttccagg gtgcgggcgg atggtcgctg gatacgctgc tgaaaaaacg
     3361 gttttgcccc caatgccgtc tgaagcaaga ttgattcagt cgtggaatct gactttaaac
     3421 attccaacct tatctcgtta acttgatatt ctgaaaagga aatagaaatg aacaaaaaca
     3481 ttgctgccgc actcgccggt gctttatccc tgtctttggc cgccggtgca gttgctgcca
     3541 acaaaccggc aagcaacgca acaggcgttc ataaatccgc ccatggctct tgcggcgcgt
     3601 ccaaatctgc cgaaggttcg tgcggcgcgg ctggttctaa agcaggcgaa ggcaaatgcg
     3661 gcgagggcaa atgcggtgcg accgtaaaaa aaacccacaa acacaccaaa gcatctaaag
     3721 ccaaggccaa atctgccgaa ggcaaatgcg gcgaaggcaa atgcggttct aaataatccc
     3781 accccttcaa accaagccgc gtttttcagt aaaatgcggc ttttttaacg gcaaacaaag
     3841 attttttaac aagcacatca ttcttttgtg ccatccgaac cgggtaaaaa tatgattcaa
     3901 cacgcaggct tgggctaccg ccgcgacttg gcggaagact ttctctcgct ttccgaaaac
     3961 agcccgatat gctttatcga agccgcaccg gaaaactggc tgaaaatggg cggctgggcg
     4021 cgcaaacagt ttgaccgtgt ggcggaacgg ctgccgctgg cgttgcacgg attgtctatg
     4081 tcgctgggcg ggcaagcacc gctggatact gatttgatag acggcatcaa agaaatgatg
     4141 cgccgttacg attgcacgtt tttctccgac catttgagct actgccacga cggcggtcat
     4201 ctttacgatt tgttgccgct gccctttacc gaggaaatgg tgcatcatac ggcgcggcgt
     4261 atccgcgaag tgcaagaccg tttgggctgc cgcatcgccg tggaaaacac gtcctactat
     4321 ctgcattccc cgcttgccga gatgaacgag gtcgagttcc tcaacgccgt cgcacgtgag
     4381 gccgattgcg gcattcatct ggatgtgaac aatatctacg tcaacgccgt caatcacggc
     4441 ctgttgtcgc cggaggcttt tttgaaaaac gttgacgcag atcgcgtgtg ctatatccat
     4501 attgccggac atgacgtgga aacgccggaa ttgttgattg atacacatgg cgcggcagtt
     4561 ttgccgactg tttgggactt gctcgaactt gcctatgcca agctgccgac gattccgccc
     4621 accctgttgg aacgcgattt taatttcccg ccttttgccg aactcgaagc cgaagtcgcc
     4681 aaaatcgccg attatcaaac gcgtgccgga aaggaatacc gccgtgcagc ctgaaacctc
     4741 cgcccaatac cagcaccgtt tcgcccaagc catacgcggg ggcgaagccg cagacggtct
     4801 gccgcaagac cgactgaacg tctatatccg cctgatacgc aacaatatcc acagctttat
     4861 cgaccgctgc tataccgaaa cgcggcaata ctttgacagc gaagaatgga gccgtctgaa
     4921 agaaggtttc gtccgcgacg cgcacgccca aacgccctat tttcaagaaa tccccggcga
     4981 gttcctacaa tattgccaaa gcctgccgct tttagacggc attttggcac tgatggattt
     5041 tgaatatacc caattgctgg cagaagttgc tcaaattccg gatattcccg acattcatta
     5101 ttcaaatgac agcaaataca caccttcccc tgcggccttt atccggcaat atcgatatga
     5161 tgttaccgat gatttgcatg aagcggaaac agccttgtta atatggcgaa acgccgaaga
     5221 tgatgtgatg taccaaacat tggacggctt cgatatgatg ctgctagaaa taatgggttc
     5281 ctccgcgctt tcgtttgaca ccctcgccca aacccttgtc gaatttatgc ctgaggacga
     5341 taattggaaa aatattttgc ttgggaaatg gtcaggctgg actgaacaaa ggattatcat
     5401 cccctccttg tccgccatat ccgaaaatat ggaaggcaat tccccgggcc aaaaccatct
     5461 atccgcataa aattaccttg ttcccgatac tatgccgcta cccgacctga ccgatgccga
     5521 attaatagag tcgcgtaaac tgcttctgca ttttgcgcgg cttcagttgc ccgaccaccc
     5581 tgatttggct gaagatttag tgcaggaaac attgctgtcc gcatacagcg caggcgacag
     5641 ttttcaaggc agggcacttg tcaacagctg gctttttgcc atattgaaaa acaaaattat
     5701 tgacgcatta cgtcaaatcg gaaggcagag gaaagtcttt accacactgg atgacgagct
     5761 actggatgaa gcatttgaaa gccatttttc ccaaaacggg cattggacgc cggaagggca
     5821 gccgcaacat tggaacactc cggaaaaatc attaaacaac aacgaattcc aaaaaattct
     5881 gcaaagctgc ctatacaacc tgcctgaaaa caccgcacgg gtatttaccc tgaaggaaat
     5941 actcggtttt tcatccgacg aaatacaaca aatgtgcggt atcagcacgt ccaactacca
     6001 caccattatg caccgcgccc gagaatcatt gcgccaatgc ctgcaaatca aatggttcaa
     6061 ccaagaaaac ccgaagtaaa cgttatgaaa aaatgccgcg atatcgccct gcttctttcc
     6121 aaacatcagg accgggaaac caccccgggc gagaagattt ccatatacac acacctgctg
     6181 ttctgtccgt attgccgtga atataaaaga caacttcaaa ccatcaaaag atcactggca
     6241 aaaacaacca gaacttcaaa ataaatgccg tctgaaaagg cttcagacgg cataagctga
     6301 cggaaacaaa tcaaaccgat ttactgttat cggcagttca tccataatac acgcttcaaa
     6361 agcagcatat tcctccatac ggaatatata aatacgcaaa acacgaaggc tgcatcaatt
     6421 tgctttattt gccttatttc acagacggcg ctacccctcc cgcccaaccc gttctttctg
     6481 aatgagcaga tttcaatgat taaggaaacc ctaatgcgcc caatcttcct atctttcgtt
     6541 ttactcccta ttttgataac cgcctgcagc acaccggaca agtctgcccg atgggaaaat
     6601 atcggcacaa tctcaaacgg caatattcat acatatatca ataaagacag cgtgagaaaa
     6661 aacggaaatc tgatgatttt ccaagataaa aaagttgtta ccaatctaaa acaagaacgt
     6721 tttgccaaca cccccgcata caagactgcc attgccgagt gggaaatcca ctgcaacaac
     6781 aaaacatacc gcttaagttc gctacagttg tttgatacaa aaaacacgga aatttccaca
     6841 caaaactaca cagcctcttc cctccgcccg atgagcatcc tgtccgggac attaaccgaa
     6901 aaacaatatg aaaccgtatg cggaaaaaaa ctctgattgc aacttataca caaacttacc
     6961 cacaaacctt atcataaaaa tgccgtctga aatactgaaa tatcagcatt tcagacggca
     7021 ttttgccatt ccctgaaaat tatccacaaa gttatccaca ttatttttta aaaccggctt
     7081 ccatccg
//
LOCUS       DAWKST010000107         7007 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.107, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000107 DAWKST010000000
VERSION     DAWKST010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7007
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.107"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(75..1496)
                     /locus_tag="WJL87_001480"
     CDS             complement(75..1496)
                     /locus_tag="WJL87_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ7799898.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINTMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(1854..2213)
                     /locus_tag="WJL87_001481"
     CDS             complement(1854..2213)
                     /locus_tag="WJL87_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ7799899.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2210..2815)
                     /locus_tag="WJL87_001482"
     CDS             complement(2210..2815)
                     /locus_tag="WJL87_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ7799900.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVIENGLEGIVQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2862..3344)
                     /locus_tag="WJL87_001483"
     CDS             complement(2862..3344)
                     /locus_tag="WJL87_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ7799901.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3548..3645
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3856..4956
                     /gene="gcvT"
                     /locus_tag="WJL87_001484"
     CDS             3856..4956
                     /gene="gcvT"
                     /locus_tag="WJL87_001484"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ7799902.1"
                     /translation="MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVHNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLGKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEADVRVLKLPFVRNGQKQFD"
     gene            5119..5505
                     /gene="gcvH"
                     /locus_tag="WJL87_001485"
     CDS             5119..5505
                     /gene="gcvH"
                     /locus_tag="WJL87_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ7799903.1"
                     /translation="MSSNIPAELKYVASHEWLRLEEDGIITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDPYGAGW
                     FFKLKPANVADYDVLLTAEQYAGEVD"
     gene            5657..6904
                     /locus_tag="WJL87_001486"
     CDS             5657..6904
                     /locus_tag="WJL87_001486"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ7799904.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAACLPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEISPYLYTLGMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHEYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIRALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSIQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
BASE COUNT         1783 a         1677 c         1974 g         1573 t
ORIGIN      
        1 tgtcggaagg ggggatattg atttgattat gccggaattt aaaatgccgt ctgaatgttc
       61 agacggcata gcgtttacag cagtttgaaa acgaaaaaga taagggtatg tacgatgaag
      121 acgggtgtca ggaaggcgac cgaccacatc atatagccga agaaagtcgg catcggtacg
      181 ccgcgctgtt cggcaatggc cttgaccatg aagttcggtg cgttgccgat gtaggtcagt
      241 gcgcccatga ataccgaacc catagaaacc gccagcagcg aatgaaacag ggtacccgtc
      301 atcaaggctt gggcatcgcc gcccgccata ttgaaaaaaa cgagataagt gggcgcgtta
      361 tccaagaatg ccgacaatat gccgctcatc caaaaataca tcgtattaat cggatgacct
      421 gccgtatcgt gaaccagcga taccaccccg cccagcgcgc ctgcctcgcc tgctttcaga
      481 atgctcagga cgggaaagat ggtgatgaag atgccgagga agagtttgcc cacttcggcg
      541 atgggttcaa agttgaattc gttgcctgcg cggacttgtt tgggcgtgat tgccatagat
      601 acggcggtca atgcaatcag gatgacatcg cggacgaggt tttgcagggc gtaacggctg
      661 ccgaggattt caaatcccgg gtgttcgggt ttccaaaggc cggacattag aaccgcgccg
      721 accacgcccg aaagcaggag gaagttccat ttgccgaaga tggcgatttt ttcgggtttt
      781 tcctgttgtg ccggcgtatc ttgtgcaatg ctttcctgtt tgaagaaacg gttgtcgatg
      841 aaatagaagg cggtcaacag gacagcggtg ctgatcagga cgggggcgaa catatgtttg
      901 accgtccaca tgaaatctac gcctttgagg aagccgagga agagtggggg gtcgcccaaa
      961 ggggtcagac cgccgccgat gtttgcaacc aggaaaatga agaagatgac gatgtgcacg
     1021 cggcgggtac ggttttggtt ggctttcagc agcggacgaa tcatcagcat tgctgcgccg
     1081 gtcgttccca tgatagaggc aagtgccgta ccgacggcaa gcagggcggt gttgagcttg
     1141 ggtgtgccgt tcaagtcgcc ccaaaccaaa atgccgcctg aaatggtgta cagggcaagc
     1201 agcagcagga tgaaggggat gtattcttca acgagtgcgt gtgcgacggt atggataccg
     1261 gcggacgcgc caaaaaccaa actgaacggg atgaggaaga gcaatgtcca aaaggcggtg
     1321 attttgccgt aatggtgatg ccaggtatgc gaaaaaaaca agggacccaa tgcgatagac
     1381 agcaaaatca gggcaaaggg caggctccac agcaggttta ggtttgcgcc gtccaaatct
     1441 gcggcgtaaa ccgatgcggg gaaaagcatt agtgaaaaca ggggtaggtg gcgcatcgtg
     1501 tttcctcgat tcaagcactg ccttgcgcgg cgcgtgggag tgatacaggc accgtgccgc
     1561 ccggacatag gcggactgtg ctgcctgttt gtttttgaaa aaagttttcc gattgattgt
     1621 aaagtaaata atcaggataa accagtttcc caaaccggaa ggcggcggga aggcggattg
     1681 ctgtgcttgg gaatatatgt tctttttgat aaataatttt atttaaaaca aataaatata
     1741 tgtctttaat aaataatcta tcgaaaacga aaaatgaatt tattttaaca tatatttgca
     1801 atgaaacagg tttgcccccc gtttgtttgc ccttatccct ttcagtacgg cattcaagat
     1861 tcgggcctgc gccacatcca tatggcgaca agggaacaaa aaaccgatga aaccgccccg
     1921 acccaccagc gttggggaaa ctgccaaaac attatcaggc aggatgcggt catcatactg
     1981 atggcgaata ttttggcttt gcgcggcact gcgccgtttt gttcccagtt atgaaccatc
     2041 ggaccgaaat agcggtgccg gtgcagccag cggtgaaagc gcggggatgc ctttgcccag
     2101 caggcggcgg agagcagtac gaacggcgtg gtcggcaaca gcggcaaaaa aatgccgatg
     2161 atacccaaca gcagggagat gcagccgcag gcaattaaaa gataacgtat cattttgaaa
     2221 tatttttctt attgtgcgga taagggcagg atgtgatacc gagttttgcc cagccttcat
     2281 gtcccatttt ttccagcagg gcgatattgc gttcgaatat tgccgaagcg tcgggaaagg
     2341 cttgtgcggc tttggcaatg ctgtcttcgc ggatgaggtg cagcgtcgga tagggagaac
     2401 ggttggtgta gttgccgatg tcgtcgggtt cggtattctg aaagcggaat tggggatgga
     2461 acggtgcgat ttggacgatg ccttccaagc cgttttcgat aacggcggca tcggcaatat
     2521 cgagcatatc gttgaatacg tcgaaatcgg ggaatagggt cgggtgaacc agcagggtgg
     2581 tttccagttc ggtggcgggt gtattgccca gtcgctgcag ttcttcgtcc aagtcttcca
     2641 aaaaactgtc aaggtgtttg gcttcgctga ttgcgatgcg gacaaggttt ttaacgtggg
     2701 gggctttggc aaagggacac aggttcagac cgatgacggc tttttccaac cattgtccgg
     2761 tgtgttcggc aacagcatct ttattttcgg aagtattgat attcattatt gtcatgtaaa
     2821 tgtgtttgca gattgcacgt gcgggaaaat cgggaagggc actattcctt cagcaggtgg
     2881 ttgagcggca gggaggtggt gtgtttgatt tcttttaaaa caaagctcga ttgcgcatct
     2941 tgtacgccgt ggtgggacag gagcgtatcc aaaacaaaat gggaaaacgc gttcatatcg
     3001 gtaaaaaacg cctgaagcag gtagtcggtt tcccctgtca gggcgaagca gctcaagact
     3061 tcaggccatt ttcgaaccga tgcggcaaag tcttcccgcg cgtcttttgc tttgcggatg
     3121 gaaacgcgga taaatgcctg aagccccagg ttgacggatt ccggagacag cagcgcggca
     3181 tattggcgga cgatgccggc atcttccaac tgcttcagac ggcgcaggca cggagaaggc
     3241 gaaagtgcga cacgttcgga cagttcgaca ttggtcagcc tgccgttttc ctggagaacc
     3301 tgtaagattt taatatcggt tttgtctaaa gtgagttggg gcatatttgc gttccgtttt
     3361 aaggaattcg gattgtctgt ccgtatgttt gcggcaatcc gcacagatgg agaccatatt
     3421 aacatataaa aagttatacc gtcatccggg acaaattttg ttttcggaaa atcatgtgaa
     3481 aacagaggcg gtcggtttgc atctctttta agacggcttg cccaaaccgc cgattcaaga
     3541 cataatcggg aaatgtgcag gagagtgtta cacccaacta caatgtaacc accgaaggcg
     3601 cagacaccct taaatcgctc aggtatcagg gactgcacat tgaaacaaac aatctggaga
     3661 gcggcgttgg aataacgtcc accgaagggg agaaggccgt ctgaaccacc attcagacaa
     3721 ccgcgcaaag cagtgagcag actggtttgc catcatgcgg atacggccga aaatctcagg
     3781 ttcaaggaca gatagggtca tccgcgcaca ggtgcgcggg tggcatctga acaaaaaatc
     3841 cggagaaact tgagaatgat tactctgaaa accaccccat ttcatcaagc ccatcaagat
     3901 gcaggcgcga agccggtaga ttttgccggc tgggagctgc ccatccatta tggttcgcaa
     3961 atcgccgaac acgaagccgt gcgcaccgac gccggtatgt ttgacgtatc ccatatgctt
     4021 gttaccgacg tagcaggcgc aaatgccaaa gcctttttcc gcaaattgat tgccaacgat
     4081 gtcgccaagc tcgcttttgt cggcaaagcc ctttattccg ctttgctcaa cgacaacggc
     4141 ggcgtgattg acgacttgat cgtttaccgc accaatgaag ccgaaaccca ataccgcatc
     4201 gtgtccaacg gcgcgactcg cgaaaaagat acggcacaat tccacaaagt cggacaagag
     4261 ttcggtgttt ccttcaatcc gcgctacgac ctcggcatgc tcgccgtaca aggccctaaa
     4321 gccattgaaa aactcctgac cgtcaaaccc gaatgggcag atgtcgtcca taacctcaaa
     4381 ccgttccaag gcgcggattt gggcaacgac tggtttgtcg cccgcaccgg ctacaccggc
     4441 gaagacggcg tcgaagtcat cctgcccggc accgaagccg tcgcattctt caaagccctg
     4501 caagcagcag gcgtacaacc ttgcggtctc ggcgcgcgcg acaccctgcg catggaagcc
     4561 ggcatgaacc tctacggcaa cgatatggac gacgatacca gcccgctcga agcaggcatg
     4621 ggttggaccg ttgatttgaa agacgaaagc cgcgattttg tcggcaaagc tgccttgttg
     4681 gcattgaaag aaaaaggcgt tgccgtcaaa caggtcggtt tgctgctcgg caaaggcggc
     4741 atcctgcgcg cgcatatgga agtgttgacc gacaaaggca aaggcgaaac caccagcggc
     4801 gtattctctc caagtctgaa acaatccatc gccatcgccc gcgtaccgaa agattttgac
     4861 ggcgataccg ccaaagtgct gattcgcggc aaggaagcgg acgtgcgcgt actgaagctg
     4921 ccgttcgtcc gcaacggtca aaaacagttt gattaatatc ggttcagacg gcattttcat
     4981 ttcatatgcc gtctgaaagc aggttttaat tgtcgtccga tacggacgtt tgtagaaagc
     5041 attgaacaag gcatctgtgg atattgattc atgcagatgc cgtctgaaaa taacccccta
     5101 tcaatggagt atcaaaccat gagcagcaac atcccggcag aactgaaata cgttgccagc
     5161 cacgaatggc tgcgccttga agaagacggt atcatcaccg tcggtattac ccaccacgcg
     5221 caagagctgt tgggcgacat cgtgtttgtc gagctgcctg aagtcggcgc aaaccttgcc
     5281 gccgaagaac aagccggtgt ggttgagtct gtaaaagccg cgtccgacgt gtacgcaccg
     5341 attgcaggtg aagtcgttgc cgtcaacgaa gatttgccaa gcgctccgga aactgccaac
     5401 agcgatcctt acggtgcagg ctggttcttc aaactcaaac cggcaaacgt tgccgattac
     5461 gacgttctgc tgactgccga acaatacgcg ggcgaagtgg attaaaccgc ccggctgccc
     5521 gatggcaacc gccggacaaa cggaaactgc accttcagac ggcatttttg cggtcggagg
     5581 tgcagttttt tgtccgtgtt ttaaggaagc agttaggcta taataacggt ctatattcat
     5641 ctttaccgat tttttcatgc aacttaccgc tgtcggactc aatcatcaaa ccgcaccttt
     5701 aagcatacgg gaaaagctgg cgtttgccgc ggcctgcctg cccgaagccg tccgcaatct
     5761 tgcccgaagc aatgcggcaa cggaggcggt aatcctttct acctgcaacc gtaccgagct
     5821 ttactgtgta ggtgattcgg aagaaatcat ccgttggctc gcagactatc acagccttcc
     5881 catagaagaa atcagcccct acctttatac tttggggatg caggagactg tgcgccatgc
     5941 tttccgcgtc gcctgcggct tggattcgat ggtgttgggc gagccgcaga ttttaggaca
     6001 gattaaggat gcggtcaggg ttgctcaaga gcaggaaagt atgggtaaga aactcaatgc
     6061 cctgttccaa aaaacctttt ctgttgctaa agaggtccgt accgatactg ccgtcggcga
     6121 aaactcggtt tccatggctt ccgcttccgt caagttggca gagcagattt tccccgacat
     6181 cggcgatttg aatgtcttgt ttatcggtgc gggtgagatg attgagctgg ttgccactta
     6241 ttttgccgcc aaaagtcccc ggctgatgac ggttgccaac cggacgctgg cgcgtgcaca
     6301 ggagttgtgc gacaagctcg gtgtcaacgc cgaaccgtgc ctgctgtccg atctgcctgc
     6361 cattttgcat gagtacgacg tggtggtttc ttcaacggca agccagttgc ccattgtcgg
     6421 caaaggtatg gtggagcgcg cattgaaaca aaggcagagt atgccgttgt ttatgcttga
     6481 cttggccgtg ccgcgagaca ttgaggcgga agtcggagat ttgaacgatg cctatcttta
     6541 tacggtggac gatatggtca atatcgtcca aagcggcaag gaggcaaggc agaaggccgc
     6601 cgccgccgcc gaaacgctgg tgtccgagaa ggttgccgaa tttgtcaggc agcagcaggg
     6661 caggcagagt gtcccgttaa tcagggcatt gagggatgag ggagagaaag cgcgcaaaca
     6721 ggtcttggaa aatgcgatga aacagcttgc caaaggcgca acggcagaag aggttttgga
     6781 aaggctgtcg atccaactga ccaacaagct gctgcattcg ccgacccaaa ccttgaataa
     6841 ggcgggggaa gaagataaag atttggttca cgccgtcgcg cagatttatc atttggacaa
     6901 ataacggtgc gccggaaaag gatgccgtct gaaggggttt cagacggcat tttttgtgca
     6961 ggccggcaac atcgtgaaat cccacattat atcgatgtaa tcacaaa
//
LOCUS       DAWKST010000108         6971 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.108, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000108 DAWKST010000000
VERSION     DAWKST010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6971
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.108"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            337..636
                     /locus_tag="WJL87_001487"
     CDS             337..636
                     /locus_tag="WJL87_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ7799905.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFFFAALTVLKLKGYQAGLYTLLIALAVAVFGFGMPAGMAVSSLPPQPH"
     gene            576..812
                     /locus_tag="WJL87_001488"
     CDS             576..812
                     /locus_tag="WJL87_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ7799906.1"
                     /translation="MRLRDAGGYGGFFPAAAAALTQRPYATLDLTAHYKIGKSTRIGL
                     DFENVFNKRYRPMPDIHVYGTPRSLTATVKHIVD"
     gene            complement(1004..2407)
                     /gene="mtrE"
                     /locus_tag="WJL87_001489"
     CDS             complement(1004..2407)
                     /gene="mtrE"
                     /locus_tag="WJL87_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ7799907.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANGSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPEDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(2462..5665)
                     /gene="mtrD"
                     /locus_tag="WJL87_001490"
     CDS             complement(2462..5665)
                     /gene="mtrD"
                     /locus_tag="WJL87_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ7799908.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            complement(5677..6915)
                     /gene="mtrC"
                     /locus_tag="WJL87_001491"
     CDS             complement(5677..6915)
                     /gene="mtrC"
                     /locus_tag="WJL87_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ7799909.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEANLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPAVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFIVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGITGAKKVTPKEWAPSENQA
                     AAPQSGVQTASEAKTASEAE"
BASE COUNT         1626 a         1951 c         1817 g         1577 t
ORIGIN      
        1 atcaaacatc aaattggact gaccaatcag ggcggattct aatgacacgc cgttcccgcc
       61 gtcaacggca tttacctcgc accgcccccg aaacacaaga aaaaactaca ccaaactaca
      121 atttttgttt atacaaatat ttgttttgac aggatttaaa cgaaagctcc gattcaaatc
      181 tgccgaaccg cccaaaaata tatagtggat taacaaaaat caggacaagg cgacgaagcc
      241 gcagacagta cagatagtac ggtaaggcga ggcaacgccg tactggttta aatttaatcc
      301 attctattga cctgaatatt aaagtttcgc aaagtaatgc aacgttgctt taattggttt
      361 gaccactatt gccgacgatt agaaaaatat tttcggagat gttcaattat ggaaacttgg
      421 gttcaaaact acacggcaat cggcggcagc ctgtatctga ctgccgccgc cgcactctta
      481 cccatcgtct ttttctttgc cgcgctgacc gtcctgaagc tgaaaggcta tcaggcgggg
      541 ctttatacgc tgctgattgc gcttgccgtt gccgtattcg gcttcgggat gccggcgggt
      601 atggcggttt cttccctgcc gccgcagccg cactgaccca acgcccctac gccacgctgg
      661 atttgaccgc gcattacaaa atcggcaaat ccacccgcat cggtttggac tttgaaaacg
      721 tgttcaacaa acgctaccgc cctatgcccg acattcacgt ttacggcacg ccgcgcagcc
      781 tgaccgcaac cgtcaaacat atagtggatt aacaaaaatc aggacaaggc gacgaagccg
      841 cagacagtac aaatagtaca acttggtgct tcagcacctt agagaatcgt tctctttgag
      901 ctaaggcgag ccaacgctgt actggttttt gttaatccac tataaagaaa gaaatgccgt
      961 ctgaaacctt atcgtttcag acggcatttt attgcccgac cggttatttg tcggtttggg
     1021 tatcccgttt caatccgccg ccgagtgcct tgtacaaatc ggcaaggttt tcggcgcggg
     1081 tcagttgtgc cgacaaagcc gcaccttccg ccgaatagct gctgcgttcc gcatcgagca
     1141 agtcgagcgc gccggatacg ccgtgtttgt aacgcagacc gaccaaacgc aacgcttctt
     1201 tagaggcgcg gctttgtttg cttaaagcgt cataggcttt atccaactgc tcgcgcgccg
     1261 ccaatgcgtt tgccacgtct tgaaatgcgg attggacggc ggattcatag gcaacgattt
     1321 gtgcctgttg gcgcagcttg gctacatcaa ggttcgcctt gttcgtaccc caggtaaaaa
     1381 tcggcagggt aatagacggc gcgaacgacc aaacgcccgt gccgcttttg aacaacccac
     1441 ccaattcggc agaacccgta ccgacggttc cggtcaggcg gatggatggg aaaaaggcgg
     1501 cgcgtgccgc accgatattg gcgtttgcct gtttgagcgc gtgttcggca gcacggatat
     1561 cgggacggtc gagcaatact tcggaactca aaccggccgg cagtttttca acaaaaaact
     1621 gcttgtccag cggcaaaccg gcaggcaggt cttcgggtat cggttggtta atcaaggttg
     1681 ccaaggcatt gcgcgcctgt tcgcggctgc gcgcggcatg ggcataatcg gctttggcag
     1741 attcgattag ggcttcctgc tgacgtaggg cgacggcgga aatcacgcct gccttgtaac
     1801 gtaattcgga cagcttgtag gtttcctcgc gcgttttcaa aacacgttgc gccaaagaca
     1861 tcgcttcttc ggcgtaacgt tcgttgaaat aggctttggc aacggtggca atcaggctca
     1921 aatgtgccgc atcgcggttg gcggtgctgg cgaaatagcc ttgcagtgcc gcctcgctgc
     1981 tgctgcgtac acgcccgaac agatcgagtt cgtaagatgc cgcacccagt ccgactttgt
     2041 agctgctgct tacattgccg ccgctcaagc tgccttggcg cgagccgttc gcattggcgg
     2101 caagcgtggg caggaggttg ttgcgctcaa tcatgtattg tttgcggtag atttcgctgt
     2161 tcaatacggc ggtacgcaaa ctggtattgc gctcgagtgc gatgtcgatc agcttttgca
     2221 ggcgcgggtc ggcaaaatag tcgtgccaac ccaaatcgac ggcgcggatg ccgctgtcgg
     2281 cggtatcgtt tttgaacgtt tcggcaactt cgactttggg ctgctcgtat tggggaatca
     2341 tggtgcaggc ggataatgcg aaggctgctg caacagaagt caaggtggtt ttcaatgtag
     2401 tatccataaa aaagtcctga tgccgtctga aaacccgtgg gcgttcagac ggcatggttg
     2461 cttaatgttg gctgtcgtcc gaaccggtga tgcccgcttc ggcagcgtgt tttactgcca
     2521 tttcgtgttc gtgcgcggtt tctttgaaga atttgcgcac caccacatag aaaagcggaa
     2581 caaggaacac cgacaagagc gtgccgatga gcatccccca gaatacggtt gtaccaatgg
     2641 cgcgctggct ggcagaactt gcaccgccgg caatatacag gggaaccacg cccaaaataa
     2701 aggcaaacga ggtcatgata atcggacgga aacgcaggcg ggctgcttcc aaagcggctt
     2761 caaccgcgct tttcccttgc gcttgaaggt ctttggcaaa ttcgataatc aaaatcgcat
     2821 ttttcgcact caaacccatc acggtaacga aaccgacttg aaagtagatg tcgttggcga
     2881 acgagggaac gctgcccaac agtccttcaa acaggttgcg cccggttacg cccgcagccg
     2941 caccgatcaa acccaacgga atcacaagga tgaccgccag cggaatcgac cagctttcat
     3001 aaagcgcggc aagtaccaaa aatacggctg caaccgccaa accgtacaaa atcagggttt
     3061 gcgagccgcc ttttgcctct tcgcgcgact gtccgcccca ttcgaagctg taaccgccgc
     3121 ccaattcgtc aaccattttt tgaaccgccg ccatagcctg cccggtggaa acgccggttg
     3181 caggcgaagc ggacagcttc atcgaaggat aaccgttgaa gcgtacgctc tgttccgtac
     3241 cgttttccca agaaacagta gcaatggtgg aaagcggtac ggcgacgccg gatttgttcg
     3301 gcacggtcag gttcaaaata tcggcaggct gcatacgggc atcctcgtcg gcctgcacca
     3361 tcacgcgttg cagacggcct tggttcggga agtcgctgac ataagacgaa ctcagcgcgc
     3421 ttgccaatgc ggtgcggatg tcggcaaacg aaatgccttg cgccgccgcg gcggcacggt
     3481 tgatgtcgat tttcaactgc ggcgagtctt ccaaaccgcc agcacggacg gtgctggggt
     3541 caaacaaacc gctggcacgc attttctgaa tcaactcgtt gcgcttcgcc agcaatgcgg
     3601 tatggccggt attattgcgg tcttgcaggt tgatgctcag gcccgaacca ttgcccaact
     3661 ccagaatcgg aggcgggacg acggcgatgc caaaaccgtc tttaagcgtc cccatcatca
     3721 tacccgtcag cttgccggca atcgcaacgg catcgctgcc gggcgcggta cgctcgttcc
     3781 aatctttcaa tatggcaaaa cccatcgcca tattctgacc gctgcccgaa aagctgaagc
     3841 cggaaacggt aatgatgttt tctatttcag gaatgctttt cgccagttgg gtaacttgcg
     3901 ccaaagtcgc attggtgcgc tcttgggtcg ctcctgcagg cagttgcacg ctgaccatga
     3961 cgaagccttg gtcttcggtc ggcaggaatg aagtcggcag gcgcataaac aggaacacgc
     4021 ccacaaccgc caagccgata tagacaacca tcatgcggaa agtcttacgc agcactttgg
     4081 caacccggcc ttcgtaaccg tgcgtccaac tgttgaattt cttgttaaac cagccgaaga
     4141 aacctttttt ctcttcgtga tgccctttcg ggattgtctt caacattgtg gcacacaaag
     4201 caggggtaag ggtcagcgca aggaaggcgg agaatgcgat tgatgacgcc atcgtcaggg
     4261 caaactgttt gtaaatattg cccgtcgccc cgctgaacat cgccaacggt acgaacacgg
     4321 aaatcagaac ggcggtaata ccgatgaccg cgcccgaaat ctgacccatc gcttttttgg
     4381 tcgcttcttt gggcggcaag ccttcacccg ccataatgcg ctcgacgttt tcaaccacca
     4441 caatcgcgtc atcgaccacg atgccgatga ccaaaaccat cgcaaacatg gtcagtacgt
     4501 taatcgacat gcccatataa gagatgaagg cgaaaccgcc caacagcgaa atcggtacga
     4561 cgatggtcgg aatcagcgta taacggatgt tttgcaggaa gagatacatt acgacaaaca
     4621 ccagcaccat cgcttcgatt aaagtatgaa tcactttttc aatcgaaatt tcgacgaatt
     4681 tggaagtatc gtaaggggtt ttccagctca taccctgagg aaagtatttt tccaacgtcg
     4741 ccatgcgttc tttaaccgcc tttgccgtcg ccatcgcatt gccgctgttg gacagcatca
     4801 ccgccatacc ggtggtattt acaccgttca gacgggttga ggaagaatag tcttccatac
     4861 ccagtccgac ccttgccaca tccttcaggt aaacattaga accgtcggta ttggcgcgga
     4921 ggatgacgtt gccgaattct tctgccgtac ccaactgccc ttgcgccgtt acggtagccg
     4981 taaccgtctg tccgcgaacg gcgggaagcg aaccgataga acccgctgaa atctggacgt
     5041 tctgggcgga cagcgcactg ccgatatcgg caaacgacaa attgtagttt tgcagtttct
     5101 taggatcaac ccaaatccgc atcgcgcgtt gcgcgccgaa cagacgtacc tgccccacgc
     5161 cttcgatacg ctgcaactcg ggaacgatat tacgctgcgc gtagtcgttc atctcttcgg
     5221 ttgactgcac atccgacgaa agcatcacaa tcatcaggaa attggaacgc gccttggata
     5281 cggttacgcc gtattgctgg acggttgccg gcagcgtgct caatacttcg gaaagcttgt
     5341 tctgcacttc cacctgcgcc agattctcgt cggtatcggg cgtaaaggtc aggctcacgc
     5401 tgccgctgcc gctcgaatcg gcggaagtgg acatataatc caaaccttcc acgccgttca
     5461 tattccgctc gatcacggaa agcacgctgt cttccattac ctgcgcggac gcacccggat
     5521 aagtggcgtg caaagtaatg gtcggggcgg cgacagacgg atattgcgaa acaggcaggc
     5581 ttttgatgcc gaaaataccc gccgcaataa tgaaaatcga aataacccac gcaaaaatag
     5641 ggcggtcgat aaaaaattta gccatcgatg ccttccttat tccgcttcag aagcagtttt
     5701 ggcttcagat gccgtctgaa cgccggattg aggcgcggcg gcttggtttt cagacggcgc
     5761 ccattctttg ggcgttacct ttttcgcacc cgttataccg gcgatactga tgccttccac
     5821 aaccaccttg tccccgtcct tcagacccga cgtaacaatc caattcgtac cctgctgttg
     5881 cgcgaccgtt acctcgcggg gttccatacc gccttgggca ttcacaatca tcacggtatc
     5941 tttcgcaccg cgcgttactg cctgctgtgg aacgatgaat gcattatcca ccgccacttg
     6001 gtccatcagc acgcgcacat acaggccggg catcaggata ttctgatcgt tcggtacggc
     6061 ggcgcgcagg gtaatctgac cggtcgattc gttgacggcc ggatcggcaa acagcaggcg
     6121 gcctttttca gggtaaactg tgccgtcgtc aaatttgatg ccgaccgcaa tcacaccatc
     6181 cgccgccagc agtttgcctt cggctatctg acggcgcagt ttcatcactt cggatgcaga
     6241 ctgggtaacg ttcacataca tcggattggt ttggcggatg gttgccagca cggtcgtatc
     6301 gcccgcattc aacagcgtac cttcggaaac tttggactga ccgataaagc cggaaatcgg
     6361 cgcggtaatg cgcgaacggt tcagattaat gccggcagat ttgattgccg cctgtgctgc
     6421 tttgacacct gcctcggcag aacgtttcgc cgttaccgca gcatcgtatt cctgccggct
     6481 gacggcttcg gcggcaacca aaggcttgta tcgcgccaaa tccgcatccg ctttggcaag
     6541 cgttgcctga gccgttgcca gttgcgcgcg cgcgctttcc agatttgctt cataagtgga
     6601 actgtcgatc tgatacagcg gctgtccggc acggacataa ctgccttctt ggaacaggcg
     6661 tttttggata atgccgccaa cttgcgcacg gacatcggcg gtacgcagcg attccaaacg
     6721 tcccggcaac tcgacggtca acgcaacggt ttgcggatgg acggttacga caccgacgac
     6781 gggagccggt gcttcccgac ccgcaggctg cccgccctgc gccgcgtctc cgcctttacc
     6841 gcaagacgac agtaccaatg caacggcggc agccaacgcg gccgcacgca tcgccttaaa
     6901 agcataaaaa gccattattt atcctatctg tctggtttga tgtaaagggt tttgccaatc
     6961 aacaggcatt c
//
LOCUS       DAWKST010000109         6877 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.109, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000109 DAWKST010000000
VERSION     DAWKST010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.109"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            98..814
                     /locus_tag="WJL87_001492"
     CDS             98..814
                     /locus_tag="WJL87_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ7799910.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            963..1865
                     /locus_tag="WJL87_001493"
     CDS             963..1865
                     /locus_tag="WJL87_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ7799911.1"
                     /translation="MDSLMTLLSVLVPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFAFGKLMRDIWMPSESAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASTDGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSTLG"
     gene            1908..2681
                     /locus_tag="WJL87_001494"
     CDS             1908..2681
                     /locus_tag="WJL87_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ7799912.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EEAGSLKRHHGGASSGGNLPEGLPADRQTRCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAVVSELVRRRGSLRVITNNIHAASIASARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2793..4481
                     /locus_tag="WJL87_001495"
     CDS             2793..4481
                     /locus_tag="WJL87_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ7799913.1"
                     /translation="MLNKDQFADNHFIRTFIEEDLKSGKHETIQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFETGRTQSRRAAFHPNHEEMGEREVPISQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTSVFNRTVTLKDSWQPK"
     gene            4574..5530
                     /gene="gshB"
                     /locus_tag="WJL87_001496"
     CDS             4574..5530
                     /gene="gshB"
                     /locus_tag="WJL87_001496"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ7799914.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQAMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
     gene            5842..6225
                     /locus_tag="WJL87_001497"
     CDS             5842..6225
                     /locus_tag="WJL87_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ7799915.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            6340..6813
                     /locus_tag="WJL87_001498"
     CDS             6340..6813
                     /locus_tag="WJL87_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ7799916.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMSAMDHGHHHGEAHQH"
BASE COUNT         1626 a         1831 c         1897 g         1523 t
ORIGIN      
        1 aatttgtgca atgctttaat tcctctgaca cgatttgaaa cacagccgcc ttaaaacatc
       61 ttaaaaaatt tgaaagcgta aaaatacgga aaacatcatg aactttgaaa acgacgacat
      121 tatccatgcg ccgaccacgt cttccctgat tctcgaagag cggcacgatt cggagctgtt
      181 ccgtgtttac gccctgattt tggacggcat taccgatcag gtgctgctgc ccggcaaaaa
      241 gctgaccgaa tccgaacttt gccgtcagat ggtgtgttcg cgcaacaccg tccgcggcgc
      301 gctgtcgctt ttggcgcacg acaagattgt cgatttgcaa cccaacaggg gcgcgttcgt
      361 ccacgttccc gatttgaaag aaatgcagga tgtgttcaat gcgcgcatcg aaatggagac
      421 gatgattttg aatatcctcg cagatttgcc ggatttggaa acgcgcctca agccgcttta
      481 tgcgatgata aggcgcgaag aagaggcttc cggcaggggc gaccgcgtcg gctggaaccg
      541 cctgtccaat gccttccacg tcgaactggc gcgcctggtg ggcaacgatg tgctgttcga
      601 cattatgaac acgctgtgcg cgcggtcttc cctgattgtc gccgtggcgg gcgtgcatcg
      661 cgaggaaaaa cacgccatca atacgcatac gcattccgaa caccgcgaaa tcctcgacct
      721 gctgctggcg ggcaggcgca accgcgtggt caaaatcctg cgccgccatt tgggcaactg
      781 tatggaacgc ttggaaaaga ctttggaaga ttgaatgccg gagcggataa gccgcctgaa
      841 ccttcaggcg gttttttaat ggcgaacctg atgccgtctg aaatatggat gcgggtatct
      901 gcaattttca gacggcaatt tttaaaccgc atatatcatg cggcaataaa ggaggtcagg
      961 agatggacag cctgatgaca ttgctttcgg tattggtacc gatgttcgcc ggatttttta
     1021 tccgtgtgcc caagccttac ctgcccgctt tggacaaggt gctgtcggtt ttggtgtatg
     1081 ccgtgctgct gctgatcggc gtatcgttgt cgcgcgtgga ggatttgggt tcgcggttgg
     1141 acgatatggc gttgacggtt ctgtggctgt ttgtttgtac ggtcggggcg aacctgcttg
     1201 ctttggcagt gttgggaaag ttattcccgt ggcggataaa ggggaaaggg aagggcgttt
     1261 cggtcggcgt gtcgggcagt gtggggcagc tcggatgcgt gctgctcgga tttgcattcg
     1321 gcaaactgat gcgcgatatt tggatgccgt ctgaaagcgc gggcatgtat tgtctgatgc
     1381 tgctggtgtt cctcatcggc gtacagctca aaagcagcgg cgtatcgttg cggcaggttt
     1441 tggtcaaccg caggggtatt cggttgtcgg tctggtttat gctttcatct ctttcgggcg
     1501 ggctgctgtt tgccgcatcg acagacggtg tgtcgtggac gaaaggtttg gcgatggctt
     1561 ccggcttcgg ttggtattcc ctctcgggtt tggtcatgac cgaggcttac ggcgcggtat
     1621 ggggcagcat catgctgctg aacgatttgg cacgagagct gtttgcactg gcatttatcc
     1681 cgctgctgat gaagcgtttt ccggatgcgg cggtgggggt tggcggtgcg accagtatgg
     1741 attttacatt gcccgtgatt cagggtgcgg gcggtttgga agtcgtgccg gtagcggtca
     1801 gcttcggcgt ggtggtcaat attgccgccc cgtttctgat ggtggtgttt tccacgctgg
     1861 gctgaacacg gtaaaatcgg catcccgatg caaggaggca gaaaacgatg aaaccgaaaa
     1921 tccaaaggca cggagagatt ttaagccttg tccgccggca tcagtttatg tcggtggacg
     1981 agcttgccgc cgcattggac gttaccccgc agacgatacg ccgcgacatc cgcgagttgg
     2041 aggaggccgg cagcctgaaa cgccatcacg gcggcgcatc ttcgggcgga aacttgccgg
     2101 agggcctgcc cgccgaccgc caaacccggt gtcaaaacga aaaaaacgcc attgcccggc
     2161 tgattgcgga acacatcccc gacggttcgt cgctgtttgt cagtatcggc acgacgatgg
     2221 aggccgtggt gtcggagctg gtcaggcggc gcggcagcct gagggtgatt accaacaata
     2281 tccacgccgc ctcgattgct tcggcgcgga cggattatac ggtcattatc acttctggag
     2341 tcgtccgtcc tttggacggc ggtattaccg gcgtggcaac cgtcgatttt atcaaccagt
     2401 tcaaagtcga ttatgccgtg atgagtacgc acggcgtgga aagcgacggt tcgcttttgg
     2461 acgacgacta caaagaagtc agcgtgatgc aggcgatgat tgccaatgcg cgcgtccgct
     2521 tcctcggcgt ggaccacagt aaattccgca gcaacgcgct ggtcaggctc ggcgacatta
     2581 cggcgtttga caaagtattt accgaccggc tgcccgatac cgcgatgcag aagatgctga
     2641 aagaggcggg ggtggaatgc ctgattgccg atgccgtctg aacgctatgt caaagcgcgc
     2701 aagtcgggta caataaacac atcatcaaac cgcttcagac ggcataagga accgccccaa
     2761 tgccgtctga agccatctgt tcaaagaaaa ctatgctcaa taaagaccaa tttgcggaca
     2821 accattttat ccgcaccttc atcgaagaag acctcaaaag cggcaaacac gaaaccatcc
     2881 aaacccgttt tccgccggag cccaacggct acctgcacat cggacacgcc aaatccatct
     2941 gcctgaactt cggtttggcg tatatttacg acggcttgtg caacctgcgt tttgacgata
     3001 ccaaccccga aaaagaaaac gacgaatacg tcaacgccat caaagaagac gttgagtggc
     3061 tcggtttcca ttgggcgggc gagccgcgtt tcgcttccaa ctatttcgac cagctttatg
     3121 actacgccgt cggtttggtt aaagacggca aagcgtatgt cgatgatttg acgccagaag
     3181 aaatgcgcga ataccgcggt acgctgaccg aagcaggcaa aaacagccct tatcgcgacc
     3241 gcagtatcga agaaaacctc gacctgttca cgcgcatgaa aaacggtgag ttccccgacg
     3301 gcagcaaaac cctgcgcctg aaaatcgaca tggcatccgg caacatcaat atgcgcgatc
     3361 cggtcatcta ccgcatccgc cgcgcccatc accacaacac cggcgacaaa tggtgcatct
     3421 atccgatgta cgactacacg cattgtattt ccgatgccat cgaaggcatc acgcattcct
     3481 tgtgtacgct cgaatttgaa gcccatcgtc cgctgtatga ctgggtgttg gacaacatcc
     3541 ccgcgccgca cgccacacgt ccgcgccaat acgagttttc acgcttggag cttttgtaca
     3601 ccattacctc caaacggaaa ttgaatcagt tggttgtgga aaaacacgtt tccggctggg
     3661 acgatccgcg tatgccgacc atttccggta tgcgccgacg cggctacacg cccgaagggc
     3721 tgcgcctgtt tgccaaacgc gccggtattt ccaaatctga aaacatcgtc gatatgagcg
     3781 tgttggaagg cgcgattcgc gaagagttgg aaaattccgc cccacgcctg atggcggtgt
     3841 tgaacccgct caaagtgacc ctgaccaact ttgaaaccgg cagaacccaa agccgccgtg
     3901 ccgcgttcca tccgaaccac gaggaaatgg gcgagcgcga agtacctatt tcacaaacca
     3961 tctatattga agccgacgac tttgccgaaa atccgcccaa aggcttcaaa cgcctgattc
     4021 ccggcggcga agtacgcttg cgccacggct atgtcatcaa gtgcgatgaa gtcgtcaaag
     4081 acgaagcagg caatgtggtt gaactcaaat gcagcatcga ccacgacacc ttgggcaaaa
     4141 atccagaagg ccgtaaagtt aaaggcgtga ttcattgggt ttccgccgaa cacgccgccg
     4201 aaatcaaagt ccgtctgtac gaccgcctct ttaccgtcga gcgtcccgat gccgtgcgcg
     4261 gcgaagacgg cgaatacctg ccgttcaccg atttcctcaa tccggaatcc gtcaaggaaa
     4321 tcaccgccta cgccgaacct gccgccaaag atttgccggc agaaagccgt tggcagttcg
     4381 agcgtatcgg ctacttcgta accgaccgta aagaccacgg caaagacacg tcggtgttta
     4441 accgcacggt cacgctgaaa gattcttggc agcctaagta aaaccccatc cttgccgtct
     4501 gaatattgtt cggacggcat ttcccctttg cccgcgaaat gcggcacatt cggcacaccg
     4561 gaaaggaaat atgatgaaag tcctctttat cgccgacccg atggcaagtt tcaaaaccta
     4621 caaagacacc acctacgcca tgatgcgcga gatggcaaaa cgcggttggc ggctgtttca
     4681 taccttgagc ggggaattgt cggttaaaaa cggtctggtg gttgctgatg cgtctgcttt
     4741 tgagtttctc ggcgcacaag acgaccacga caaaaactgg tttgccgccg ccgacaaagt
     4801 tcagacggca ttgaaagcat ttgatgccgt gattatgcgt accgatccgc cgttcgatat
     4861 gcaatacctc tacgccacac agcttctgac gctggcggag cagcagggcg cgaaggtctt
     4921 taacagcgga caggcgatgc gtgatttcaa tgaaaaactg gcgattttga acttcagccg
     4981 ctttaccgcg cccacgctgg taacgacccg ttccgccgat gtccgcgcat ttttgaaaga
     5041 acacggcgac atcatcatta aaccgctgga cggtatgggc ggtatgggta ttttccgcct
     5101 gaccgaaaaa gatcccaaca tcggcagcat cctcgaaacc ctgatgcggc ttgattcccg
     5161 caccattatg gcgcaacgct acattcccga aatcgtacac ggtgacaaac gcatcctgat
     5221 tatcggcggc gaagtcgtcc cctatgcttt ggcgcgtatc ccgcaaaacg gcgaaacacg
     5281 cggcaacctt gcggcaggcg gacgcggcgt ggcgcaggaa ttggacggac gcgaccgcga
     5341 aattgccgaa acgctcgccc ccgaactcaa acggcgcggc atcctgctgg ccggtttgga
     5401 cgttatcggc agcaacctga ccgaagtcaa cgtaaccagc ccgaccggat tccaagaaat
     5461 tatgaaacaa aaaggtttcg acgtggcggc aatgtttgcc gatgccgttg ccgcgtggtc
     5521 ggtacgttaa accgatgccg tttgaaaggc ttttgcttca caaccgtttg gtttgttcgg
     5581 gcaggcaggg ttttctctca gccggcaggg atgcggttaa tgccgtacgg acaccccgcc
     5641 gccccgtttt cagacggtat atatagtgga ttaacaaaaa tcaggacaag gcggcgaagc
     5701 tgcagacagt acaaatagta cggaaccgat tcactcggtg cttcagcacc ttagagaatc
     5761 gttctctttg agctaaggcg gggcaacgcc gtacaggttt ttgttaatcc actatattga
     5821 gaacattttg aaaggatacc gatggaacct tcctcctacg cggcagaaaa aaaaggcaaa
     5881 ggcggcatca ggcgcgtcat taacgcattc ggctattcga tagacggcat cgccgccgcc
     5941 taccgttacg aagcggcatt ccgtcaggtt ttgtggctga acgtactgct ggtgtgcgcg
     6001 gcattttttt gggtttccga aaccgccgtc cgcctgccgt tgattatcgc gtcttttgtg
     6061 tcggtcattg tcgaactgtt caacaccgcc gtcgaagctg ccgtcgatca tacttcgacc
     6121 gaaaaacacg agctggccaa acgcgccaaa gacgcaggtt ctgccgcaca attgttcgcg
     6181 atgctgatgt tggcggcggt ttggctgtct gccctgttcg ggtaaaacgc ttgcagcagg
     6241 atttgtaatc ctttaggatt agtatgtggt atttattatt cacttaatcc actataagat
     6301 taagcacatt ccccgtctga tattataagg agcagaaaga tgaaaaaatt attggcggcc
     6361 gtgatgatgg caggtttggc aggcgcggtt tccgccgccg gagtccacgt tgaggacggc
     6421 tgggcgcgca ccaccgtcga aggtatgaaa ataggcggcg cgttcatgaa aatccacaac
     6481 gacgaagcca aacaagactt tttgctcggc ggaagcagcc ccgttgccga ccgcgtcgaa
     6541 gtgcataccc acatcaacga caacggcgtg atgcggatgc gcgaagtcaa aggcggcgtg
     6601 cctttggagg cgaaatccgt taccgaactc aagcccggca gctatcacgt gatgtttatg
     6661 ggtttgaaaa aacaattaaa agagggcgac aagattcccg ttaccctgaa atttaaaaac
     6721 gccaaagcgc aaaccgtcca actggaagtc aaaatcgcgc cgatgtcggc aatggaccac
     6781 ggtcatcacc acggcgaagc gcatcagcac taatctgctg aaaatatttg aaatgccgtc
     6841 tgaaaaagcc cgggctttca gacggcattt ttatgac
//
LOCUS       DAWKST010000110         6823 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.110, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000110 DAWKST010000000
VERSION     DAWKST010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6823
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.110"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            120..1025
                     /gene="glyQ"
                     /locus_tag="WJL87_001499"
     CDS             120..1025
                     /gene="glyQ"
                     /locus_tag="WJL87_001499"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ7799917.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTLDGNTVTYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            1102..1512
                     /locus_tag="WJL87_001500"
     CDS             1102..1512
                     /locus_tag="WJL87_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HEZ7799918.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQTLIFGVQGSLHQAE
                     DDAEAFDRFMDELIAYLAANGLEMAGGGDCGDFSCLFQKSGSRGLGETEKKAIISWLA
                     GREDVATLHATPLVDGFYGDDGWENYSETYKQAD"
     gene            1533..3596
                     /locus_tag="WJL87_001501"
     CDS             1533..3596
                     /locus_tag="WJL87_001501"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ7799919.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAMKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGGLLEDIINQAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSDGEIIIENADSYAEQMREQGKVVA
                     SFAERKAAIQTALEGQAHRLNATVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLANVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDNLPNGKVA
                     TAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAALGEVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            3660..4700
                     /locus_tag="WJL87_001502"
     CDS             3660..4700
                     /locus_tag="WJL87_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ7799920.1"
                     /translation="MQPLVSVLICAYNVEKYFAQSLAAVVNQTWRNLDILIVDDGSTD
                     GTLAIAKDFQKRDSRIRILAQPRNSGLIPSLNIGLDELAKSGMGEYIARTDADDIAAP
                     DWIEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHEHGKIWKKPTRHEDIAAFF
                     PFGNPIHNNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSKLGRLAYYPEALVKYRL
                     HANQVSSKHSVRQHEIAQGIQKTARNDFLQSMGFKTRFDSLEYRQTKAAAYELLEKDL
                     PEEDFERARRFLYQCFKRADTPPARAWLDFAADGRMRRLFTLRQYFSILHRLIKNRRQ
                     ARSDSAGKEQEI"
     gene            4700..5539
                     /locus_tag="WJL87_001503"
     CDS             4700..5539
                     /locus_tag="WJL87_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ7799921.1"
                     /translation="MQNHVISLSSAAERRAHIAETFGSRGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSSVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGEGAEKFLD
                     EDAWLQERFDKDSAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISK
                     KAMRFFLDRFAALPSEGLHPVDWMMFGNPDDREGMPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQRRDSPANTFKHRLIRALTKISREREKRRQRREQLIGKIIV
                     PFQ"
     gene            5551..5646
                     /locus_tag="WJL87_001504"
     CDS             5551..5646
                     /locus_tag="WJL87_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HEZ7799922.1"
                     /translation="MDIVFAADGRMRRLFTLRQYFGILRRLLKNR"
     gene            5688..6494
                     /locus_tag="WJL87_001505"
     CDS             5688..6494
                     /locus_tag="WJL87_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ7799923.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSSVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGEGAEKFLD
                     EDAWLQERFDKDSAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISK
                     KAMRFFLDRFAVLPSERLKAVDWMLFSSFLDKGGMTVCQLTPALCIQSETLPSQLKNG
                     RQESYRNRRSPKVLLKRALGKIGREIERARERKRQKKLEKHLGRHVVPFE"
BASE COUNT         1714 a         1888 c         1796 g         1425 t
ORIGIN      
        1 aatttaatcc actatatcat gtatgccgtg ttttcaaatc agtctgtctt tgagataaac
       61 ctatccaaac agcaatggaa acagtaaaat aaacccttta ttttatcgaa tcataagcca
      121 tgcttacctt ccaacaaatc atcttcaaac tgcaaacctt ctgggcagac aaaggctgca
      181 ccgtcatcca acccttcgac atggaagtcg gtgccggcac atcccatccc gccacctgcc
      241 tgcgcgcgct cgggcccgag ccttggtttg ccgcctacgt ccaacccagc cgccgcccca
      301 aagacggccg ctacggcgac aaccccaacc gcctgcaaca ctattaccaa ttccaagtcg
      361 ccctcaagcc cgctcccgct aatatccaag acctctatct cgactctctg cgcgaattgg
      421 gcatcgatcc caaagtccac gacatccgct ttgtcgaaga cgactgggaa aaccccaccc
      481 tcggcgcgtg gggtttgggc tgggaagtct ggctcaacgg catggaagta acccagttta
      541 cctatttcca acaagtcggc ggcatcgact gcacgcccgt actcggcgaa atcacctacg
      601 gcatcgaacg cttggcgatg tacttgcaag gtgtagaaaa cgtctacgac ctcgtttggg
      661 caaaaacgct cgacggcaac accgtcacct acggcgacgt gtaccaccaa aacgaagtcg
      721 agcaatccac ctacaacttc gaatacagcg atgccgactg gctgctgcgc cagttcaacg
      781 actacgaagc gcaagccaaa cgcctgcttg ccgaagaaaa cgccgccctc gccctgcctg
      841 cctacgagct tgtcctcaaa gcggggcata cgttcaacct tttagacgca cgcggcgcga
      901 tttccgtaac tgagcgggca acttatattg gacgtattcg tgcattgagc cgcgccgtgg
      961 cgcaaaaata tgttgaaagc cgcgaaaaac tgggcttccc tttaatgaag gcaaacgccg
     1021 cctaaggggg cgacgatttt ttacgttcgg gcggcacgat aaggcagccg tccggaagcc
     1081 attatatttt ttaggaaaat catgcctgtc tatttccgca ccccaagcag gaaacgcctg
     1141 cacaaaatga accgccgcca gcataaaaag ctgcatttgg gcgagtttca aacattgatt
     1201 ttcggcgttc aaggcagttt gcaccaagct gaggacgatg ccgaagcgtt cgaccgcttt
     1261 atggacgagc tgattgcgta tcttgcggca aacggcttgg aaatggcggg cgggggcgat
     1321 tgcggcgatt tttcgtgcct gtttcaaaaa tccggctccc gaggcttggg cgaaaccgag
     1381 aaaaaagcca ttatctcttg gctggcaggt cgcgaagatg tcgccacttt gcacgccacc
     1441 ccgctggttg acggttttta cggggatgac ggctgggaaa attacagcga aacctataaa
     1501 caggcagatt aaccaaattc aaagaaagcc gaatgatgac ccaaaccctt ttaatcgaac
     1561 tccttaccga agaactcccg cccaaagctc tgaataatct gggcaaccat tttgccgccg
     1621 ccgttgccga aggcttggaa aaagcgcaac tggttgacgg tgcagccgaa tttacggctt
     1681 atgcctcgcc gcgccgtttg gcggttcaag tcaaaaacgt caaagccgtt caggccgacc
     1741 aaaaaatcgt gaaaaaaggc cctgccgtgg cgaatgccat gaaagacggt gcgccgacca
     1801 aggctttgga aggtttcgca cgcggtgcgg gcgcgaaaat cgaagatttg accatcgtcc
     1861 acgacggcaa gcaagacgtg tacgcctacg aatacgtcca aaccggcaaa ccgttgggcg
     1921 gacttttgga agacattatc aatcaagccg ttaagaaact gccgattcca aaagtgatgc
     1981 gttggggcag cagtacattt accttcgtgc gccccgtcca cgggctgatt gtgctgcacg
     2041 gcggcgacat cgtaaacgtc agcgttttgg gtctgcaaag cagcaatcaa accttgggac
     2101 accgcttcct ctccgacggc gaaatcatca ttgaaaacgc cgacagctac gccgaacaaa
     2161 tgcgcgaaca aggcaaagtc gtcgcttcgt ttgccgagcg caaagccgcg attcagacgg
     2221 cattggaagg gcaggcacac cgtctgaacg cgaccgttgc cgccgatgaa gcattgttgg
     2281 acgaagttac cgcgttggtc gaatggcctg tggtattgga agccggtttt gaagaacact
     2341 tcctcgccgt gccgcaagaa tgcttgattc tgaccatgca gcaaaaccaa aaatacttcc
     2401 cgctgctcga ccaaaacggc aagctgatga accgcttcct gctggtctcc aacctgcaaa
     2461 ccgaagatcc gtcgcacatc atccgaggca acgaacgcgt tttgcgcgcg cgcctgtctg
     2521 atgccgaatt cttctacaag caagaccaaa aggcgacttt ggaaagccgc ctgcccaagc
     2581 tggcaaacgt ggtttatcac aacaaaatcg gctcgcaagc cgaacgcatc gaacgtttgc
     2641 aaagcattgc cgcccatatc gccaaagcat tgggcgcgga tgccgcggca gccgaacgcg
     2701 ccgcgcgttt ggcgaaagcc gacttggtga ccgaaatggt cggcgagttc cccgaactgc
     2761 aaggtacgat gggcaaatac tatgcccgtt tggacggcga aaccgaagaa atcgccgaag
     2821 ccatcgagca acactatcaa ccgcgttttg ccggtgataa cttgccgaac ggcaaagtcg
     2881 ccactgccgt ggcactggcg gacaagctgg aaaccttggt cggcatttgg ggcatcggtc
     2941 tgattccgac cggcgacaaa gacccctacg ccctgcgccg cgccgccttg ggtattttgc
     3001 ggatgctgat gcagtatggt ttggacgtga acgaactgat tcagacggca ttcgacagct
     3061 tccccaaagg tttgctcaac gaaaaaacgc cgtctgaaac cgccgacttt atgcaggcgc
     3121 gccttgccgt gttgctgcaa aacgattatc cgcaagacat cgttgccgcc gtactcgcca
     3181 aacagccgcg ccgtttggac gatttgaccg ccaaactgca ggctgtcgcc gcgttcaaac
     3241 aactgcccga agccgccgcg ctcgccgccg ccaacaaacg cgtgcaaaac ctgctgaaaa
     3301 aagccgatgc cgcgttgggc gaagtcaatg aaagcctgtt gcaacaggac gaagaaaaag
     3361 ccctctttgc cgctgcgcaa ggcttgcagc cgaaaatcgc cgccgccgtc gccgaaggca
     3421 atttccaaac cgccttgtcc gaactggctt ccgtcaagcc gcaagtcgat gccttctttg
     3481 acggcgtgat ggtgatggcg gaagatgccg ccgtaaaaca aaaccgcctg aacctgctga
     3541 accgcttggc agagcagatg aacgcggtgg ccgacatcgc gcttttgggc gagtaaccgt
     3601 tgtatagtcc aaatgccgtc tgaagccttc agacggcatc aaattatcgg gagaataaat
     3661 tgcagccttt agtcagcgta ttgatttgcg cctacaacgt agaaaaatat ttcgcccaat
     3721 cattagccgc cgtcgtgaat cagacttggc gaaacttgga tattttgatt gtcgatgacg
     3781 gctcgacaga cggtacgctt gccattgcca aggattttca aaagcgggac agccgtatcc
     3841 gcatcctcgc gcagccgcgc aattcgggcc tgattccttc tttaaacatc gggctggacg
     3901 aattggcaaa gtcaggaatg ggggaatata ttgcacgcac cgatgccgac gatattgccg
     3961 cccccgactg gattgaaaaa atcgtgggcg agatggaaaa agaccgcagc atcattgcca
     4021 tgggtgcgtg gctggaagtt ttgtcggaag aaaaggacgg caaccggctg gcgcggcacc
     4081 acgaacacgg caaaatttgg aaaaagccga cccggcacga agacatcgcc gcctttttcc
     4141 ctttcggcaa ccctatacac aacaacacga tgattatgcg gcgcagcgtc attgacggcg
     4201 gtttgcgtta caacaccgag cgggattggg cggaagatta ccaattttgg tacgatgtca
     4261 gcaaattggg caggctggct tattatcccg aagccttggt caaataccgc cttcatgcca
     4321 atcaggtttc atccaaacac agcgtccgcc aacacgaaat cgcgcagggc atccaaaaaa
     4381 ccgccagaaa cgatttcttg cagtctatgg gttttaaaac ccggttcgac agccttgaat
     4441 accgccaaac aaaagcagcg gcgtatgaac tgctggagaa ggatttgccg gaagaagatt
     4501 ttgaacgcgc ccgccggttt ttgtaccaat gcttcaaacg ggcggacacg ccgcccgctc
     4561 gtgcgtggct ggattttgcg gcagacggca ggatgcggcg gctgtttacc ttgaggcaat
     4621 acttcagcat tttgcaccgg ctgattaaaa accgccggca ggcgcggtcg gattcggcag
     4681 ggaaagaaca ggagatttaa tgcaaaacca cgttatcagc ctaagctccg ccgcagaacg
     4741 cagggcgcac attgccgaaa ccttcggcag tcgcggcatc ccgtttcagt ttttcgacgc
     4801 actgatgccg tctgaaaggc tggaacaggc gatggcggaa ctcgtccccg gcttgtcggc
     4861 gcacccctat ttgagcagtg tggaaaaagc ctgctttatg agccacgccg tattgtggaa
     4921 acaggcattg gacgaaggtc tgccgtatat cgccgtattt gaggacgacg ttttactcgg
     4981 cgaaggcgcg gagaaattcc ttgacgaaga cgcttggctg caagagcgtt ttgacaagga
     5041 ttccgccttt atcgtccgct tggaaacgat gtttatgcac gtcctgacct cgccctccgg
     5101 cgtggcggac tactgcgggc gtgccttccc gcttttggaa agcgaacact gggggacggc
     5161 gggctatatc atttccaaaa aagcgatgcg gtttttcctg gacaggtttg cagccctgcc
     5221 gtctgaaggg ctgcaccccg tcgattggat gatgttcggc aaccctgacg acagggaagg
     5281 aatgccggtt tgccagctca atcccgcctt gtgcgcccaa gagctgcatt atgccaagtt
     5341 tcacgaccaa aacagcgcat tgggcagcct gatcgaacac gaccgcctcc tgaaccgcaa
     5401 acagcaaagg cgcgattccc ccgccaacac attcaaacac cgcctgatcc gcgccttgac
     5461 caaaatcagc agggaaaggg aaaaacgccg gcaaaggcgc gaacagttaa tcggcaaaat
     5521 tattgtgcct ttccaataaa aggagaaaag atggacatcg tatttgcggc agacggcagg
     5581 atgcggcggc tgtttacctt gaggcaatac ttcggcattt tgcgccgatt gctgaaaaac
     5641 cgttgaaaaa cgccgcttta tccaacagac aaaaaacagg ataaattatg caaaaccacg
     5701 ttatcagctt ggcttccgcc gcagaacgca gggcgcacat tgccgatacc ttcggcaggc
     5761 acggcatccc gtttcagttt ttcgacgcac tgatgccgtc tgaaaggctg gaacaggcaa
     5821 tggcggaact cgtccccggc ttgtcggcgc acccctattt gagcagtgtg gaaaaagcct
     5881 gctttatgag ccacgccgta ttgtggaaac aggcattgga cgaaggtctg ccgtatatcg
     5941 ccgtatttga ggacgacgtt ttactcggcg aaggcgcgga gaaattcctt gacgaagacg
     6001 cttggctgca agagcgtttt gacaaggatt ccgcctttat cgtccgcttg gaaacgatgt
     6061 ttatgcacgt cctgacctcg ccctccggcg tggcggacta ctgcgggcgt gccttcccgc
     6121 ttttggaaag cgaacactgg gggacggcgg gctatatcat ttccaaaaaa gcgatgcgtt
     6181 tctttttgga caggtttgcc gttttgccgt ccgaacgcct gaaggcggtg gattggatgc
     6241 tgttcagcag ttttctcgac aaagggggga tgacggtttg ccaactgacc cccgccttgt
     6301 gcatccaaag cgaaaccctg cccagccaac tgaaaaacgg caggcaggaa tcgtaccgca
     6361 accgccgctc cccgaaggtt ttgttgaaac gcgctttggg aaaaatcggc agggaaatcg
     6421 aaagggcgag ggaacggaaa cgtcagaaaa aattggaaaa acacctcggg cggcacgtcg
     6481 tcccgtttga ataggttttg gcctttcctt atttggcggg tgtgcgctgt ccctaaattt
     6541 atgtcgggga atccggtttg ttcggtttca cttgttttcg ggtaagccct aattcgtgat
     6601 tcccgcgaaa acagaaagcc aaaaacagaa acctgaaatc tcgtcattcc cgcgcaggcg
     6661 ggaatctagg tctttaaact tcggtttttt ccgataaatt cttgccgcat taaaattcta
     6721 gattcccgtc tgcgcgggaa tgacggcggg gggatttctg tttttccgat aaattcctaa
     6781 aacttaaaat tccgttattc ccgcgaaaac agaaaaccaa aaa
//
LOCUS       DAWKST010000111         6683 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.111, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000111 DAWKST010000000
VERSION     DAWKST010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.111"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(605..712)
                     /locus_tag="WJL87_001506"
                     /pseudo
     CDS             complement(605..712)
                     /locus_tag="WJL87_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1085..1753)
                     /locus_tag="WJL87_001507"
     CDS             complement(1085..1753)
                     /locus_tag="WJL87_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ7799924.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCHLFGITNIIGTQLESGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVDEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            complement(1750..2418)
                     /gene="hda"
                     /locus_tag="WJL87_001508"
     CDS             complement(1750..2418)
                     /gene="hda"
                     /locus_tag="WJL87_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ7799925.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            complement(2523..3140)
                     /locus_tag="WJL87_001509"
     CDS             complement(2523..3140)
                     /locus_tag="WJL87_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ7799926.1"
                     /translation="MVWPEYSPQENVALFRHNGEILLSGINIKLKSGDSLLIRGPSGC
                     GKTSLLCALAGLWPFGSSGKVSRPPHQDILFLPQRPYTAQGSLRDAVCYPDIDKQHPE
                     LAEAMNTCRLGYLIDKLDKTADWQHKLSPGELQRVAFVRALLSKPKIVLLDEATAALD
                     EPAEAALYRALKQKLPDSIIISIGHRSTLNTFHNMHLNVGNVACD"
     gene            complement(3495..4394)
                     /locus_tag="WJL87_001510"
     CDS             complement(3495..4394)
                     /locus_tag="WJL87_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEZ7799927.1"
                     /translation="MPALTVTWMIYTKYLVYQIYLVIFCGVFMERIDLALNLVEERSR
                     LGYSRKNFAEQTGCSAESLRLYESGQVNIPADFLLAAVQLGVDIQFVFTGIHSANLNA
                     VSDDLNKKLDDAIGKSNVENQINGNVSNAVIASSGSVVHQINTQNHVIRTRVDSKPGK
                     EHITLEQASKLQQLVKQVAAAEEIAKRSPKSIRAIWASLNAHCKVPSYKLIALSDYDK
                     AETYLRKWLGRLSNTATSKNNDPDWRKKKYAYIKLNVKQLELEDWLKSYLEKNFSVES
                     LTELSDEDLQKTYAAVSTKKRKK"
     gene            4438..4689
                     /locus_tag="WJL87_001511"
     CDS             4438..4689
                     /locus_tag="WJL87_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7799928.1"
                     /translation="MQANEIYKSLEEKNISARMLASALGVTNQSVSEVIRNGRGSKRI
                     AEAIAKVIQKDLVEVFPHYKKTDCRDKKIAELKEMLGVG"
     gene            4691..6670
                     /locus_tag="WJL87_001512"
     CDS             4691..6670
                     /locus_tag="WJL87_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7799929.1"
                     /translation="MEYMDLKSLLNFGLPNMPRTVIGLRKKAQRDGWQTRRRQGKGGG
                     VEYALPAEIRAAIMKRQSDELAEKMPKTLPQVRPGTAMSAQALAEAAKLLNEKQRSVA
                     DARCAVVAAVLGIKYQYDCSAKAAVAQFLGLLAEGKLDAVTLGNLEKANDRSRSAKVG
                     ERTLDGWISAYLKAENATERLVALAPKTTKAVKPIESYGWLPMFMQFHNIPSAPKLAH
                     SYRRFVQWAEAENMPVNDVPNLSMVRRVWDKLPLIMQERGRKTGAAYKSLLPYVKRDW
                     GALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAESCVAV
                     SDALRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRLGIRHETGIAGNPQGRGI
                     IERWWKDNLIEMARQYETFAGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQQKYLK
                     KLPGWSRFIADVVKCIDEYNNRPHGELPRHPDGGHYSPKAYREMRLEQDGIAPDMLSA
                     QELATMFMPQEVRKVQRGWLDLFNNSYFSVELAEYHKDEVRVSYDLSDASAVNVFDMD
                     GKFITKAQANGNTREAFPTARIDQLAEKRRKGKIKRAENAIKLANAEVNPALEQAAVW
                     DELGHLGGNVIEAEYAVLPKTGTDDGIFLFEADRS"
BASE COUNT         1699 a         1343 c         1837 g         1804 t
ORIGIN      
        1 gtcgccttgt cctgattttt gttaatccac tatacgtcct agattcccac tttcgtggga
       61 atgacgggat gtgggttttt gtgcggattt gaaccggtaa gggtggtgtg ggattggtgg
      121 tttgcttagg atcttttgga ttgtattttg tatatacatt tacttgttga taaaagataa
      181 aataaaatta gaaactaaaa gtgagaaaaa attaataata atagggatgt ataaatgtaa
      241 agactccgtt tcatagctaa ggttatctga atatatggaa aaaaagtaaa agtccataaa
      301 actaaaatat atagataatg ctaatgataa tagaattatc actttcctag agtaaggata
      361 atattttttt atcgtaaaaa aatataaaat aggataaata attgccatat agatagattt
      421 tgtaacaaaa ttaaaaataa ataataaaaa taatgtaata aagtatattt tttggctatg
      481 aaataaaatt gtacataatt gaacgagcag atcaaaaaat gaactacata taacaataaa
      541 taataacgta tttaccatac taaatttaat aggtctcatt atcatattta ataaccactt
      601 catagtatag tggattaaat ttaaaccagt acagcgttgc ctcgccttgc cgtactatct
      661 gtactgtctg cggctttgtc gccttgtcct gatttaaatt taatccacta taaattttaa
      721 tccactataa atgcagagtg ggtggaaaca ctcactttat ggtttgctac gctctgctca
      781 attagcaacc cgataaccca atatggataa tagggtaatt aatccaatct aatttgtcag
      841 catccgttaa tttattgcaa aataaagtat tgaattatgt cgggtgcaaa tgatgaaata
      901 taagtttccg tgcggacgga tcaagattcc cactttcgtg ggaatgacgg tggaaagatt
      961 gttgtttttc ccgatgaatt cctgtgtttt ttgtttttcc ggataaattc ctgtggcttt
     1021 gagttttttg gatttcagcc tcaatgccgt ctgaacgccg aatcgggctt cagacggcat
     1081 tgcgtcattt gaaattcaaa accggccagc ctttttcttt ggcttctttt tccagctcgg
     1141 catcggggtt gacggcgacg ggttcgtcga cgaggcgcag cagcggcagg tcgtttttgg
     1201 agtcgctgta aaaataggtt ttgccgtagc tttcgagcgt ttcgccgcgt tcggcgagcc
     1261 attggttcag gcgggtgatt ttaccttctt tgaggctggg cgtgccgatg taattgccgg
     1321 tgtagcggcc gtcggaaccg gattcgagtt gtgtgccgat gatgttggta atgccgaaaa
     1381 ggtggcagac gggggtgatg atgaactcgt tggttgagga aatcacaagg gtttcgtcgc
     1441 ctgccatttg gtggctctgc accagcatac gctgcatagg cgagatgtgg gggatgatgt
     1501 attccgccat aaattcgcgg tgaaattccg ccagctcttc tttgctgtaa cgggcgagcg
     1561 gggcgaggtg gaatttgagg aatgcgtcga tgtcgaggca gccgttttgg tagtcgcggt
     1621 agaatttttc gttttgcgct tcggtttcgg cagcgtcaac caagcctttt ttgatgaggt
     1681 attgcggcca ggcgtggtcg gaatcggtgt tgatgagggt gttgtcgagg tcgaagatgg
     1741 cgaggttttt cattgggttt cctgttgttt caaaagctgg cgcaaaagcg gcagggtgat
     1801 gcgtttgccc atcgtgacgg cgtagttgtc cagcgtgtcg agcatcatca tcaggctgtc
     1861 catatcgcgc cgccagtgtt tgagcaggta ttcgaaaatt tcggaatcaa cggttacttg
     1921 gcgtgccgcc gccatactgg cgagcgcgtc gattttttct tggtcggtca ggggtttgac
     1981 ttcgtaaacg aggcagtaag ccatacgcgt ccgcaaatct tcgcggatga cgagctgctg
     2041 gggcgtgtat tccgaaccga gcagcaaaaa gcctttgccg ctgttgcgga agcggttgaa
     2101 gatggaaaaa agcagggctt gttcttcgtt gcccagcttt tcgacttgat cgacggcgag
     2161 gtattccgcc tcgaacgcgg catcggtcag cggcatggag gcggcatcga tataggcggc
     2221 gtttttgccg gcttcgagcg cctgtgcgac ccacgcctgc aaaagatggc ttttgcccgc
     2281 gccttcttcg ccccaaacat aaatgaattg tccgtgtttg tgtcggagga catagaccag
     2341 ttccgcgttt tccgtgccga ggaatttgtc gaaactcgga tagtcgtgtg cggcaaagtc
     2401 gaaaataagc tggttcacgg ttcggcattc cgaggggtgg taaacgggtt tattgtacgt
     2461 tgttttcgcg cgcctttcca atttgaacga tgccgtctga aaacggcttc agacggcatc
     2521 gttcagtcgc aggcaacgtt gccgacattg aggtgcatat tgtggaacgt gttgagcgtg
     2581 cttcggtgtc cgatgctgat gatgatgctg tcgggcagtt tttgctttaa ggcgcggtag
     2641 agtgcggctt cggccggttc gtccaaggcg gcggtggctt cgtcgagcag gacgattttg
     2701 ggcttggaaa gcagggcgcg gacgaaggcg acgcgttgca gttcgccagg ggagagtttg
     2761 tgctgccagt cggctgtttt gtccaatttg tcaatcagat aacccaagcg gcaggtgttc
     2821 atggcttcgg ctaactcggg atgctgtttg tcgatgtccg gataacaaac cgcgtcgcgt
     2881 aggctgcctt gcgccgtgta cgggcgttgc ggcaggaaga ggatgtcttg atgcggcgga
     2941 cggctgactt tgccgctgct gccgaacggc caaagcccag ccagcgcgca cagcaacgaa
     3001 gttttgccgc aaccgctcgg gccgcgtatc agcagggaat cgccgctttt gagtttgatg
     3061 ttgatgccgc tcaacaggat ttcgccgttg tggcggaaca gagcgacgtt ttcctgtggg
     3121 gaatactccg gccataccac tcaacacggc ctatgatggc gatgtcgtct tgggtattac
     3181 tcaaatctat ttcaaacggt gcgtaacgtg gattttcaga cgttacaagc agtttgcccg
     3241 gtatacgttg cacacgtttg acaaagaggt cattgcctat acgcaagaca tataggccgt
     3301 cgcgcggctc ggtttcggcg tggttgatta gaatgtttat aaaaggcttt aactagaaac
     3361 tgaacatgga acttgtataa tagttccatg tttgaaaaaa tcttttaata tcatacatct
     3421 aaaaaaacaa agcatctgaa gaccataagc taaacatgaa acttgaattt aaaggcggag
     3481 tttcatgttt aagcctattt tttccgtttt ttagtcgata ctgctgcata tgttttttgc
     3541 aaatcttcat cacttaattc agtcaggctt tctactgaga aatttttttc gaggtaactt
     3601 ttcaaccaat cttcaagctc aagctgtttt acatttaatt tgatataagc gtatttcttt
     3661 tttcgccagt cgggatcgtt gttctttgat gttgctgtat tgctcaacct acccagccat
     3721 tttctgagat aagtttctgc tttgtcgtaa tcgctcaatg cgattagttt gtaacttgga
     3781 actttacagt gagcattcag actagcccaa attgcacgga ttgattttgg cgaccgtttg
     3841 gcaatctctt ctgctgcggc aacttgttta acaagttgtt gcagctttga agcttgctca
     3901 agcgttatat gttccttgcc gggttttgaa tcgacacggg ttctgatgac gtgattttgg
     3961 gtattaattt gatgaacaac cgaaccagat gaggcgataa cagcgttact gacgttgccg
     4021 ttaatctgat tctcaacgtt tgatttgcca atagcgtcgt ccaatttttt attaaggtcg
     4081 tctgaaacag catttaaatt tgccgaatga atgcctgtaa agacaaattg aatatccacc
     4141 cctaattgga cagcagcaag taaaaagtcc gcaggaatgt tgacctgccc gctttcatac
     4201 agtctcaaac tttctgcact acatccagtt tgctcagcaa aattctttcg agagtatcca
     4261 agtcggcttc tctcttcaac taaattcaat gctaaatcaa tgcgttccat aaaaacaccg
     4321 caaaatatta ccaaatatat ttggtatacc aaatattttg tgtatatcat ccaagtaaca
     4381 gttaaggcag gcattaaagc ctgtaaggta agtaatggat tctatcatgg aggcattatg
     4441 caagcgaacg aaatctataa atccttagaa gaaaaaaata tatcggcaag aatgttggct
     4501 tcggctttag gggtaactaa ccaatctgtt tctgaggtca taagaaatgg acgaggaagc
     4561 aaaagaattg cggaggcaat cgcgaaagtg attcaaaaag atttagttga agtttttccg
     4621 cattacaaga aaacagattg tcgagacaaa aagatagctg aattgaaaga gatgctcgga
     4681 gtgggttgaa atggaatata tggatctcaa aagcctgctt aattttgggt tgccaaatat
     4741 gccaagaaca gtaatagggc tgagaaaaaa agcgcaaagg gatggttggc aaacgagaag
     4801 gcgtcaaggt aagggtggtg gtgttgaata tgccctccct gccgaaatcc gagcagccat
     4861 catgaaacgg cagtcggacg agctggcgga gaagatgccg aaaaccctgc cccaagtcag
     4921 accggggacg gcgatgtcgg ctcaggcact ggctgaagcg gccaagctgt tgaacgagaa
     4981 acaacggtcg gtggcggatg cgcgatgtgc ggtggtggcg gcggtgttgg ggattaaata
     5041 ccaatacgat tgctctgcca aggctgcggt ggctcagttt ttgggcttgc tggcagaagg
     5101 taaattggac gcggtcacgc ttgggaactt ggaaaaggcc aatgaccgca gccggtcggc
     5161 gaaggttggc gaacgtactt tagacggctg gatttctgct tatttgaaag cggaaaacgc
     5221 gacggagcgg ttggttgctt tggctccgaa gacgacgaag gcggtcaagc cgattgaaag
     5281 ttacggatgg ttgccgatgt ttatgcagtt tcacaatatt ccgtccgcgc caaagctggc
     5341 gcacagctac cgccggtttg tgcagtgggc ggaagcggaa aatatgccgg tcaatgatgt
     5401 gcctaacttg agtatggtgc ggcgcgtttg ggacaagctg ccgctgatta tgcaggagcg
     5461 cggcaggaaa acgggggcgg cttataaatc gctgctgcct tatgtgaaac gtgattgggg
     5521 ggctttgaag ccgaacgatg tttggatcgg cgacggccac agctttaagg cgaaggtggc
     5581 tcatccggta cacggcaggc cgtttaagcc ggaagtgacg gtgattattg atggttgtac
     5641 gcggtttgtg gtgggttttt cggtgtcgtt ggctgaaagt tgtgtggcgg tatcggacgc
     5701 tctgcgtatc ggggtcaagc actttggttt gccgattatc tactactcgg ataacggtgg
     5761 cggccaaacc ggcaagacga tagaccatga aatcacgggt attacgtccc gattgggtat
     5821 ccggcatgaa acgggtatcg cgggcaaccc gcaaggccga ggcatcatcg agcgatggtg
     5881 gaaagacaat ctgattgaga tggcgcgcca gtatgagacg tttgcgggcg cggggatgga
     5941 cagcagtacc aagaacctga tgtaccgcaa gatggaaagt gcgtttaacg ctttggaaaa
     6001 aggcaaggat ttgacggagg aacaacagaa atatttgaaa aaactgccgg gctggtcgcg
     6061 ttttatcgcg gatgtggtca agtgtatcga cgaatacaac aaccgcccgc acggcgagct
     6121 gccccgacat ccggacggcg ggcattattc gccgaaggct tatcgggaaa tgaggctgga
     6181 acaggacggt atcgcgccgg atatgttgtc ggcgcaagag ctggcgacga tgtttatgcc
     6241 gcaagaggtg cgaaaggtac agcgcggttg gctggatttg ttcaacaact cttatttttc
     6301 ggtggagctg gcggagtatc acaaagacga ggtacgggtc agctacgatt tgagcgatgc
     6361 gtcggcggtc aatgtgtttg atatggacgg caagtttatc actaaggcgc aggccaacgg
     6421 caatacccgc gaggctttcc cgacggctcg tatcgaccaa ctggcggaaa aacgccgaaa
     6481 aggcaaaatc aagcgggcgg aaaatgcaat caagctcgca aatgcagaag tcaatcctgc
     6541 tttggaacag gctgcggttt gggacgagct gggacatttg ggcggaaacg tcatcgaggc
     6601 ggagtatgcg gtattgccga aaacgggcac ggacgacggg attttcttgt ttgaggcgga
     6661 tagaagttaa aacggtttta aac
//
LOCUS       DAWKST010000112         6682 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.112, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000112 DAWKST010000000
VERSION     DAWKST010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6682
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.112"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..410)
                     /locus_tag="WJL87_001513"
     CDS             complement(<1..410)
                     /locus_tag="WJL87_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799930.1"
                     /translation="MKADIQTELTQALLSHEKVWANEEKTILAKNILLDLVEKTDPAI
                     IGLLLGNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSVNNSYTKYANRIGLTDGNRFL
                     KDSSDIVLDFPFKDCVLNGGQSSEEGEEIYFKHS"
     gene            complement(701..1633)
                     /gene="truB"
                     /locus_tag="WJL87_001514"
     CDS             complement(701..1633)
                     /gene="truB"
                     /locus_tag="WJL87_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ7799931.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHT
                     GVLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIIATARAD
                     ISLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKPRDITVYAIDI
                     AEFDAPKAVISVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLNETERDSLLLPCDVLVAHFPQTVLNDYAVHMLQCGQRPRFEEDLPSDTPVRVYT
                     ENGRFVGLAEYQKEICRLKALRLMNTAVKNTGCV"
     gene            complement(1666..2037)
                     /gene="rbfA"
                     /locus_tag="WJL87_001515"
     CDS             complement(1666..2037)
                     /gene="rbfA"
                     /locus_tag="WJL87_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ7799932.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKTPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            2228..3472
                     /gene="clpX"
                     /locus_tag="WJL87_001516"
     CDS             2228..3472
                     /gene="clpX"
                     /locus_tag="WJL87_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ7799933.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKGDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLKGL
                     KKVVVGKAVIEEGRELELVFEP"
     gene            complement(3575..4771)
                     /locus_tag="WJL87_001517"
     CDS             complement(3575..4771)
                     /locus_tag="WJL87_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEZ7799934.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTKGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGIVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINTPETLNHVREQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCVL
                     DEAYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLNDEDMAEGLRRLEHALTEFA
                     ATSDNP"
     gene            5175..5675
                     /locus_tag="WJL87_001518"
     CDS             5175..5675
                     /locus_tag="WJL87_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799935.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHPLPL"
     gene            5760..6314
                     /locus_tag="WJL87_001519"
     CDS             5760..6314
                     /locus_tag="WJL87_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799936.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSAHPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
BASE COUNT         1616 a         1746 c         1754 g         1566 t
ORIGIN      
        1 ttactgtgtt taaaataaat ctcttcgcct tcttcgctgc tttgcccgcc attcaatacg
       61 caatctttaa acggaaaatc caacacaata tccgaactgt ctttcaaaaa gcggttgccg
      121 tccgtcaaac caatgcggtt ggcgtatttt gtgtaggaat tattgacgct gtgtttgtcc
      181 aagaaaaaac gaaaatcctg caatttaaac accaaaacac cattcacttc cacaaaaaaa
      241 tggcgtttta actcatcatt ccccaataac aaaccgataa tggccgggtc ggttttttcc
      301 accaaatcca acagaatgtt tttggctaaa atggtttttt cttcgttcgc ccatactttt
      361 tcgtgtgata gcagggcttg ggttaattcg gtttgaatgt ctgccttcat cgttataaat
      421 cctcatcagt cattttaatg tgccattctt gtgtaacggc aaatggtttt gcaaataaat
      481 actaataaaa tcaatcattt gttatctttg ctgtttccgg gggcgttcgc aaagttgcaa
      541 taagcggggt gggtgtccga gtgtgtcgtg attttgcgga aatatagtgg attaacaaaa
      601 atcaggacaa ggcgacgaag ccgcggacag tacaaatagt acggcaaggc gaggcaacgc
      661 tgtactggtt taaatttaat ccactatatc aacacattat tcaaacacag cccgtatttt
      721 taaccgccgt gttcatcagg cgcaaggctt tcagacggca tatttctttt tgatattccg
      781 ccagtccgac aaagcggccg ttttccgtgt aaacgcgtac cggcgtatcg gaaggcaggt
      841 cttcctcgaa acgcggccgt tgcccgcatt ggagcatatg gacggcataa tcgtttaaaa
      901 cggtttgggg aaaatgggca accaatacgt cgcagggcag cagcaagctg tcgcgttctg
      961 tttcgttcaa atttgccaag gcttcgagcg tgtggctttg ggcgatggta aagccggcgg
     1021 tttcggtgcg gcgcagggcg gtcaggtggg cgaacgtgcc gatgtgtttg gcgatgtctt
     1081 cgctgagggt gcggatatag gtgcctttgc tgcaacgcac gcttatcacg gctttgggtg
     1141 cgtcaaattc ggcaatatcg atggcgtaaa cggtaatatc gcgcggcttg cgttcgatga
     1201 cgatgccttt gcgggcgtat tcgtacagcg gtttgccttc gtgcttgagc gcggaaaaca
     1261 ttggcggcac ttggcggatg ttgcctgtca gtgcttggca ggccgtctga aattcggcta
     1321 aggaaatatc ggcgcgggcg gtggcaatga tttcgccttc ggcatcaccc gtgctgctgg
     1381 cttcgccgag tttcagcgtg gcggtgtagg ctttgtcggc atccagcagg tattgggcga
     1441 acttggtcgc ttcaccgaag cagacgggca aaagtccggt tgccaaaggg tcgagcacgc
     1501 cggtatgtcc ggctttttcg gcatgaaaca aacgccgcgc tttttgcagc gcggtgttgc
     1561 tggaaaggcc ttcgggtttg tccaagagca aaacaccgtt gacggcacgt ttttggggtt
     1621 tgcctgtatt catggttcga tgttcggacg gcatgggcat atccgtcagt cttcaaccgg
     1681 tttttccgcc gctacttggt cgataagggc ggacaggttc aaaccgcgtt ccaaagattc
     1741 gtcgtatttg aaatgcagtt cgggcgtttt gaacagcttg atgcgtttgg ccaattcgct
     1801 gcggaggtgt ccgcgcgcgt gttccagcac ttcttccgta atttcgcgcg cgtcttggtt
     1861 taaaatggtg tagaacacgg tggcgtggct gtaatcgcgg gtaacttcga cttcgttgac
     1921 ggtaatgaag ccggcgcgcg ggtctttcag tccggtacgg acgagttcgg caagctcgcg
     1981 catgatttgt tctttgacac ggtcttggcg ggcgtagccg cgttggggtt ttctcatgaa
     2041 tatcctcaga tttcagattt ggtttcagac ggcatttttg ccgccggggc ggttgcgaaa
     2101 gggcgtatta tagcctaatc ggcgggattt taaggtaata cattgataag aacggatata
     2161 gttattatac ttggcaaccc gaacggcacg tgccgttttc tgtaacgcat acattgtaaa
     2221 gaaagccatg tccaacgaaa accgtacctg ttccttttgc ggaaaatcca aatcacacgt
     2281 caaacattta attgagggcg aaaacgcctt tatctgtgac gaatgcgtct caaactgtat
     2341 tgaaatattg catgaagacg gaaatgacgg cacaccttcg gaaagtgccg gaggggagcc
     2401 ggaagaatcc ggcaagctgc ccacgcccgc cgaaatcgtc gccaacctca acgaccacgt
     2461 tatcggtcag gaacaagcca agaaagcctt ggcggtgtcg gtgtacaacc attacaaacg
     2521 cctgcgccac ccgaaagccg gagctaatgt cgaattgtcg aaatccaaca tcctgcttat
     2581 cggcccgacc ggatcgggta aaaccctgct ggcgcaatct ttggcgcgta aactggacgt
     2641 accgttcgtg atggcggatg caaccacgct gactgaagca ggttatgtcg gcgaagatgt
     2701 cgagcaaatc atcaccaaac tgttgggcaa atgtgatttc gatgttgaaa aagcccagcg
     2761 cggcatcgtc tatatcgacg aaatcgataa aatttcacgc aaaggcgaca atccgtccat
     2821 cacgcgcgac gtgtccggcg aaggtgtgca acaagccttg ctgaaactga ttgaaggtac
     2881 ggtggcaagt gttccgcccc aaggcggccg caagcatccg aatcaggaat ttatcaacgt
     2941 cgataccacc aacatcctgt ttatctgcgg cggtgcgttt gcaggcttgg aaaaagtcat
     3001 ccgccagcgc accgagaaag gcggtatcgg tttcggtgcg tccgttcaca gcaaggacga
     3061 aaatgccgac attaccaagc tgttcggcat cgtcgaaccg gaagatttaa tcaaattcgg
     3121 tctgattccg gaattaatcg gacgtttgcc cgtgattgca acgttagaag aactggatga
     3181 ggacgcgctg attaatattt tgaccgagcc gaaaaatgct ttggtcaagc agtatcaagc
     3241 cttgttcggt atggaaaatg tcgaactgga gtttgaagaa ggcgcattgc gttccatcgc
     3301 gcgtcaggca atggagcgca aaaccggcgc gcgcggcctg cgttccatcg tcgaacgctg
     3361 cctgttggat acgatgtacc gcctgcccga tttgaaaggc ttgaaaaaag tggtggtcgg
     3421 taaggcagtc atcgaagagg gcagggaact ggaattggtg ttcgagccct gatggcaaac
     3481 aaaaccgctt cggaacgtcg aaccacgttt ccgaagcggt tttttgtttt tgatgccgtc
     3541 tgaagccttc ccgccttcag acggcatttg agttttacgg attgtctgat gtcgcggcaa
     3601 attccgtcag cgcgtgttcc aaacgtcgca aaccttccgc catatcctca tcgttcaaca
     3661 gtagcgaagg cgcgaaacgc aatacgtccg cacccgcaac caaaatcatc acgccgtgtt
     3721 tcaaggcggc ggcggtgatt tcggatgcgc gtccgcgata ggcttcgtcc aacacgcagc
     3781 cgagtagcag ccccatcccg cgaacttgtg agaacaagcc cgttttcctg cccaaatcca
     3841 gcaatgccgt ctgaagtttc tgcccctgtt cacggacatg gtttaaagtt tcgggcgtat
     3901 tgatgatgtc gaatgcgcgg ctgccgaccg cacacgccat cgggttgccg ccgaaagtcg
     3961 agccgtgcgt gcccggttgg aaggcggcgg caatcttttc tgtcgccagc atcgcgccga
     4021 tcggaaagcc gcagcccaag gcttttgccg aactcaaaat atcgggaaca atgccgtaat
     4081 gttcataggc aaacagcctg cccgtatgcc ccatcccggt ttgaacttcg tccaaaatca
     4141 acaacgcatt gtgccggtcg cacagacggc gcgcggtttg caaatattcc gcagtggcgg
     4201 gcaggatgcc gctttcgccc tgtatcggct cgatgatgac cgcgcaggtc tgttcgccga
     4261 cggcagcttc cagcgcggca atatcgttga acggaacgtg cgtaatgcct tgcggcaggg
     4321 gtgcataatc cttgctgtat ttcggctgac cgccgacgga cacggtaaac agcgtgcgtc
     4381 cgtgaaaact gttgatacag gcgacgattt cgctttttcc tccgccgaaa cggtcgcggg
     4441 cgtatttcct cgccagcttc aacgccgcct cattcgcttc cgcgcccgag ttgcagaaaa
     4501 aaaccttgtc ggcaaaactg tttgcaacca atttttgcgc caattcctgc gccggacgcg
     4561 tcgtatagat attggaaatg tgccacagct tgtgcatctg cgcgtttaaa gcatcgacaa
     4621 gggcagggtg gcagtgtccc agcgcattga cggcgatacc gcctgaaaaa tcaatatatt
     4681 cacgcccttt cgtatcccaa acgcggctgc ctgaagcgcg ttcgggaatc atcggggcaa
     4741 aggcgaaatt gggggtcaga tagttttgca taattgctcc tgattgcggt tttgctcggg
     4801 attatgcacc gtttttcaaa gcagataaag ggaaattgcc gacaatatcg ggcagcggta
     4861 aaaataaacc gaaatatttt caatcggata gatgaaaaat caaaaaaatt tcgataaaat
     4921 gcttgaggcg cggcagcgtt ttgatttaga atctccccaa tatttttatc ttcagacggc
     4981 atttcatcat gcaaggcaaa aaatttacaa ccgcttattg ctattggtac cacacagacg
     5041 tgcggtactt ttttgcgctt gcttgaaatc catcccatac gcaaaacagg ccgctcgaaa
     5101 acggggcggc ttttttgtta ccttcacaca ggtcgcccga ttcggcaaca cacgacagaa
     5161 tcaggaaaat gaccatgaat gcaatgcccc aaaccgccgc acaaaacgaa accctgcaca
     5221 cttgtagcat tattgtcgat gatgcacagg atttttcccc catgcttctc gatgcagggc
     5281 gggacgacac acttatcagc gaatcgatga ttccgcaaat ccgcaccgtt gccgccctga
     5341 tagccgccga acggcacgac ttcagccgca gcagccccgc cgagtttacc gatgccgccg
     5401 atttttttgc cgcacggatt ctcgtattgg gcgtgcgacg cttccatctg gacgtatcgc
     5461 tgcttcagat tttgaaaacc gccaacagcc gcgcccgccg ctttgccgaa aaacaccgcc
     5521 tgtttttcac acccgcacaa gccgaattga gcctgaacaa acacaaaaac cgccgcctgc
     5581 tgaccgtaga aaccgaacac gaagtcgaaa acaaaggcaa cccagtcgcc aacagcctcg
     5641 catttgtccg caaattgcac cccctgcccc tgtaaacatc acgatttgcc ggcagaatgc
     5701 ttgccaaaat tcccgacggc gatacaataa aaccgatttt ccacccaaac aggacacaca
     5761 tgaaaaaaat catcgcctcc gcgcttatcg caacattcgc actcgccgcc tgccaagacg
     5821 acacgcaggc gcggctcgaa cagcagcaga aacagattga agccctgcaa cagcagctcg
     5881 cacagcaggc agacgatacg gtttaccaac tgactcccga agcagtcaaa gacaccattc
     5941 ctgccgaagc acaggcaaac ggcaacaacg ggcaacccgt taccggtaaa gacgggcagc
     6001 agtatattta cgaccaatcg acaggaagct ggctgctgca aagcctagtc ggcgcggcgg
     6061 caggcgcgtt tatcggcaac gcgctggcaa acaaattcac acgggcaggc aaccaagaca
     6121 gtcccgtcgc ccggcgcgcg cgtgcagcct accatcagtc cgcacatccc aatgcgcgca
     6181 ccagcaggga tttgaacacg cgcagcctcc gtgcaaaaca acaggcggcg caggcgcagc
     6241 gttaccgccc gacaacgcgc ccgcccgcca attaccgccg ccccgccatg cgcggtttcg
     6301 gcagaaggcg gtaaatccgg cgtgccaatg ccgtctgaag ggctttcaga cggcattttt
     6361 gcatttgtta ggggcattgt tatgttgccg tttgattttt agacggcatt ttgtttccaa
     6421 gcgtttgatg tcgggatggc aattctgatt tacgtgcgtt ggcagggcgg cattgtcatg
     6481 ccgccgttgg gttttcagac ggcatcatat cgaaccgtca gaccataggg cgagcattct
     6541 tgcccaccgt tgcaagggcg ggattgggtg ggcaggaatg cccaccctgc atctgaaacc
     6601 cttccgtgtc attcccgtgc aggaaggaat ctagatattt gaatttacgg aaattttcaa
     6661 tcgttgccaa agcggaaaag gg
//
LOCUS       DAWKST010000113         6609 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.113, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000113 DAWKST010000000
VERSION     DAWKST010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.113"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            14..583
                     /locus_tag="WJL87_001520"
                     /pseudo
     CDS             14..583
                     /locus_tag="WJL87_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691695.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            815..1315
                     /locus_tag="WJL87_001521"
     CDS             815..1315
                     /locus_tag="WJL87_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799937.1"
                     /translation="MVFVNPLYYGREAGWFVWLAAAMCGLWLLRALTAKLPQQRYAAL
                     FITLFFAAVLVFGRHDSMYWYPVLVNLMMLAVFGGSLFAGQTVIEKLARLQQPDLPEK
                     AVRYTRRVTQVWCVFFIANGTLAALLARLGRYDWWAVYTGVIAYVLMGMPFAGEWLYR
                     KLVLKV"
     gene            1322..1837
                     /locus_tag="WJL87_001522"
     CDS             1322..1837
                     /locus_tag="WJL87_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEZ7799938.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPYPYNQLSEEERIAYQAECGGDF
                     CIIRYPDQTDRFIRAVLLIPIIGHEETLEYGVWVSVSEKSFNDYQSHFHDNPENVVYF
                     GMICNRLPPYETDTFGLHCNVVTQPDGQRPLLQLHQGSEHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            1857..3335
                     /gene="ubiD"
                     /locus_tag="WJL87_001523"
     CDS             1857..3335
                     /gene="ubiD"
                     /locus_tag="WJL87_001523"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ7799939.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRIAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPVKPDGTRYGYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGENVDLYKLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWEELGL"
     gene            complement(3400..5514)
                     /locus_tag="WJL87_001524"
     CDS             complement(3400..5514)
                     /locus_tag="WJL87_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ7799940.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDQTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSDGIGTPKISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTAGGEYAQWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKGHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            5605..6498
                     /locus_tag="WJL87_001525"
     CDS             5605..6498
                     /locus_tag="WJL87_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEZ7799941.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADERRGRCRRLAEQTASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLSHTKDHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
BASE COUNT         1407 a         1618 c         2010 g         1574 t
ORIGIN      
        1 aatttaatcc actatagttt atgcctcgca cgacggggaa atggcgcgca gtttcgattt
       61 gtggttggaa ctgttgaaat cgcaaaccgt gtcgccgact tcgttcggtt tgtcggtgca
      121 caatgcaacg gcggggtaat ggtcgatatt gaggcgggat atgagcgagc agacggcatt
      181 ggccgtgtgt gccgacggtg tggaaacggc attggcggaa gcggcatcac tgttggaaga
      241 gggatgcggg tcggttttgg ttttggcggc ggacgatccg ctgccggaag gatatgccgt
      301 atcggcaacg cgcgcgccgt ttgcttatgc tttggcgatg gttctgacca aagggacgcg
      361 ttacagcctg actttatctg cttcggacga tatgccgtct gaagccggta tgctgcccga
      421 ggcatattgg agcggtttgg aatgggtgcg ctttctgctg aacggcagtc gggaatgccg
      481 ccgggtatat cgcaatcgtg aatggctgtg gcagcccgca agctgcaggc tgaagatttc
      541 cgcaatgtcc gcaccgtcaa cgacgtggta caggcggtat tgaagattca ggcgggctaa
      601 tcgggtattt ctaattattt ttcagacggc atgtttttat cggatgccgt ctgaaaacat
      661 ttgtgcggat tcaaaatgaa acccggtttt ttcggacata tttttctgac cgtattaagc
      721 attgcctatc ccgctttgtg gtatagtgga ttaacaaaaa tcaggacaag gcgacgaagc
      781 cgcagacagt acggcaaggc gaggcaacgc tgtactggtt tttgttaatc cactatatta
      841 cggcagggag gcgggctggt ttgtctggct ggcggcggcg atgtgcgggt tgtggctgct
      901 gcgcgccctg actgccaaac tgccccaaca gcgttatgcg gctttgttta ttacactgtt
      961 ttttgccgca gtattggtgt tcgggcggca tgattcgatg tattggtatc ccgtcctcgt
     1021 caatctgatg atgttggcgg tgttcggcgg cagcctgttt gccgggcaga cggtgattga
     1081 aaaacttgcg cggctgcaac agcccgacct gcctgaaaaa gctgtgcgct atacccgccg
     1141 ggtaacgcag gtttggtgcg tattctttat tgccaacggg acgcttgccg ctttgttggc
     1201 acggttgggg cgatatgatt ggtgggcggt ttataccggc gtgattgcct atgtattgat
     1261 ggggatgccg tttgccggcg aatggcttta ccgcaaactc gttttgaaag tctgaaacct
     1321 tatgtacact tgcacttcct gcggcgaaaa acatgaagat atgcccgcaa tcggctttac
     1381 cgccccctat ccctataatc aactcagtga agaagaacgc attgcttacc aagctgaatg
     1441 cggcggcgat ttctgcatca tccgctatcc cgaccaaacc gaccgtttta tccgcgcggt
     1501 gctgcttatt ccgattatcg ggcatgagga aacgctggaa tacggcgtgt gggtgtccgt
     1561 cagcgaaaag agttttaacg actatcaaag ccactttcac gacaatccgg aaaatgtcgt
     1621 ttatttcggg atgatctgca accggctgcc cccttatgaa accgacacct tcggtttgca
     1681 ttgcaatgtc gttacccaac ccgatggtca acgtcctttg ttgcaactcc atcaaggcag
     1741 cgaacatccg ctggtacgcg acttttatca cggcatagaa tatgccgaag cgcaggcaag
     1801 gataggggcg gtatccgaat cggattgctc aggataaaag gacaaaaagg atgaatatga
     1861 aatacaaaga cctgcgcgac ttcatcgcca tgctcgagca gcagggcaaa ctcaaacgca
     1921 tcgcgcaccc cgtttccccg catttggaaa tgaccgaaat cgccgaccgc gtgctgcgcg
     1981 ccgaagggcc ggcgttgctg tttgaaaacc cggttaagcc cgacggtacg cgctacggtt
     2041 atcctgtgtt ggcaaacctg ttcggcacgc ccgaacgtgt ggcgatgggc atgggcgcgg
     2101 acagcgtgtc caagctgcgt gaaatcgggc agacgctggc gtatttgaaa gaacccgaac
     2161 cgcccaaagg catcaaagat gcgttttcca aactgccgct actgaaagac atttggagca
     2221 tggcgccgaa cgtggtgaaa aacgcgccgt gtcaggaaat cgtgtgggaa ggcgaaaacg
     2281 ttgatttgta taaacttccg attcagcatt gctggccgga agacgttgcg ccgctggtaa
     2341 catggggctt gaccgtaacg cgcgggccgc acaaaaaacg ccaaaatctc ggcatttacc
     2401 gccaacaact catcggcaaa aacaagctga ttatgcgctg gctgtcgcat cgcggcggcg
     2461 cgttggatta tcaggagttc cgcaaactca atcccgatac gccgtatccc gtcgccgtcg
     2521 tgctcggttg cgaccctgcc actattctcg gagcggtaac gcctgttccc gatactttga
     2581 gcgaatacca gtttgccgga ctgctgcgcg gttcgcggac ggagctggtg aaatgtatcg
     2641 gcaacgattt gcaagtgcct gcccgtgctg aaattgtatt ggaaggcgtg attcatccga
     2701 acgaaaccgc gttggaaggc ccatacggcg accacaccgg ctattacaac gagcaggatc
     2761 atttccctgt atttacggtc gagcgcatca ccatgcgcga aaacccgatt taccattcga
     2821 cctacacagg caaacctccc gatgaacccg ctgttttggg cgtggcattg aacgaagtgt
     2881 tcgtaccgct cttgcaaaag cagttccccg aaatcaccga tttttacctg ccgcccgaag
     2941 gctgctccta ccgcatggcg gtggtgagca tgaaaaaaca gtacgccgga cacgccaagc
     3001 gcgtgatgat gggctgctgg tcgttcctgc gccagtttat gtacaccaaa ttcatcatcg
     3061 tggtggatga cgatgtgaac gtacgcgact ggaaagaagt catctgggcg gtcaccacgc
     3121 gcatggaccc cgtgcgcgac actgttttgg tagaaaacac gcccatcgat tatctcgact
     3181 tcgccagccc cgtcagcgga ctcggcggca aaatgggttt ggatgcgacc aacaaatggc
     3241 cgggcgaaac cgaccgcgaa tggggacgcg tcatcaaaaa agaccctgcg gttacggcta
     3301 agattgatgg gatttgggag gaattggggt tgtaggtttt acggtcatgc cgtctgaagc
     3361 gcgggggtgt ttcagacggc atttttgtac gcaggatgtt tacggttctt taggtttgag
     3421 ccactgccat tgttccagca gggtgtcttc gaggattttc tgtttctctt tgcgcgaatt
     3481 gatgccgccg gtcagcctgc cgtaatcgac ggtaatggac ggcttgtcca ccgtaccggt
     3541 gattttcagg ggaatcggtt tgtttttcgg atggacggcg ttgcggataa ggacatcttc
     3601 agacaattcc tgcgtatcca gattggtata gccgttgctg gtaacataga ggctgtcgga
     3661 gaagagttcg gtatcgatgt ggcggctgat gccgtctgaa atttcgctgt tgagcgtgaa
     3721 tcggtggaag ggtgtgctgg gtgcggcatt gtcggcagtt ttgcccgaaa tgccgttttt
     3781 caggatattg tccatgtcga taccgtgcca tgcgccattg gaaatattca gcgacaggct
     3841 gccttgaagc gagcggataa gctcttttcg ggtttcgccg cccgcggtca ggtcgatgac
     3901 cgcgtcgccg ttgccgctga agctgtggaa gccgaacagg tcttgcagca gcggttggat
     3961 ttggatgttg cttgcattct gttgcaggcg gtaagtggcg ggacgggtgt tggcgatgct
     4021 gatgccgcct tcggtatggc cgccgtaaag ccctgacttg aaacggctga gcgcgatatg
     4081 gcctttgtcg gcgtggaggt aggtttccat atcgtccagt tgcaggccgg gaagttggac
     4141 ttttccgatt ttcaggtgcg cctcgatgtc gccggacagc ttggcgaggg tgtcgggaaa
     4201 tattttgccg ttttgttgcc gcacgtcgtc aagatagggg gtcaggttca atttttgcag
     4261 tgcgacggcg gcttccagat gcggtgcgtc ttcatgtgtg tatctgaatt tcgcggcaac
     4321 ggtttggcgg tcgaatgtgc cgtttaattc ggcattccaa ttttgcagat tcggtacgga
     4381 cagcgaaccg tcgagccggc tgatgaaacg gggttgcggc aggcggttga cggtatcctg
     4441 aagggtcgat acatacaggc gcggcgcgtc cagccctttg ttttccgtcc atacgagcgg
     4501 cgaattgagg gagaagttgg tctggtggcg cggtgttttg aagctgccgg agatttcggc
     4561 gttgccgatg ttggcgatgc cggagtgcag gttggctttg tcgagtttga acgaaccgtc
     4621 ccattgggca tattcgccgc cggcggtaaa tgcgccgttg acggtttcaa ttttaatgct
     4681 gttgttcctg agtgccagcg tagggatttg ggcggtcagg tggaggttgc ggaaggaagt
     4741 gtctgcacgc aggccgaggc cggcggcatc cgctccgccg gcgttgaagc ggacagaagg
     4801 gctgccggtg gtggaaatgg taatgccgtg tccgtccagc gaagtggaag cttcaaaatg
     4861 gaacggtgag attttgggcg tgccgatgcc gtctgaaagg aacagccccc tgcttttcca
     4921 cgggacggac agctttcccc aaaccagtat gcccgaactt tcaaacggct gccccgacga
     4981 atcgggggat tgcaggttga ggttgatttc cttcaggata agctgttcct gcaggaaatt
     5041 gaggcggacg gtgctgtttt cgacgataat gcggttgact gaggcttggc gtttttggct
     5101 gtcgatcagg tcttggatgt tccaaacacc tttcccgtcg cgcgtcaggg caagttccgc
     5161 actcgaaacc acccattttt caatctgtat ctgatccgac cacaggtttt tccagctcaa
     5221 tccgattttg gtttcttgga cggaaacggc agtctggtcg ccgccgggtt cggtaatggt
     5281 caggtttttc aggatgacgg tcggccgggg caggagcctg cgctgaatgt ccgcatcaaa
     5341 cgagattttc cggtgtgtgt gtgcaatgct ttgttggagg cggctgcgga tgttttcagg
     5401 cgtgaaggtg cgatatacgg aagcgtgcag cccgactgcc gccaaaagca gtatcgtcag
     5461 cacggccacc gcatatttca gacggtattt gtggaaaacc gataataaat ccatagcccg
     5521 aacaatctgt aaaacacgcc acagtataac acggcttgca aaaatcctat tttgggcagg
     5581 ccgtttcttg tgtcaggagg aaatatgatt attgtcatga gcaggcgtgc cgccgaagcg
     5641 gacattgccg gcgttgtcgc ctttatccgc agcagaggtc tgcgagaaca catttctcac
     5701 ggcgacgagc gtaccgtcat cggcgcaatc ggcgacgata gggttttgag cgtcagggag
     5761 gtgcagacgc tgccggaggt cgagaaagcc gtccgcattt tggatacatg gaaaaccgtc
     5821 agtcgggaaa atcgggcgga agacagccgc gttgccgcca aaggcgtggc tttcggcggc
     5881 ggggaaaccg tccgtatcgc cgcagaaccg tcagtatggc gcaatgcgga tgcagtgttt
     5941 ctggatccgt ttttcacttc ggcaaactta tacgacactt caagtgcgga tgaaaggcgg
     6001 ggacggtgca gacggttggc ggaacagacc gcatcggcgc atggcgcagg gaaacccgta
     6061 ttggtgcgcg tccgcaacgt ccgccatgtc gaagcggctt tgaatgcggg ggcggacatc
     6121 ctgtatttgg gcggcgggct gatgtccgat cttgccgtat tgaatgaagc cggaagcctg
     6181 aacattccct tggtgctgtg taaagacaag caccattctg ccgaagattg gttgaatgcc
     6241 gcagaatatg tcgtctcgcg cggcaaccgc cacctgattc tgggcgagtc gggcgtgttg
     6301 agccatacca aagaccatcc ctaccgtttg gatgtcgaat ccatcgtgaa agtccgccaa
     6361 atcagccatc tgcccgtgat tgcgaatatt acgggattat ggaaccgcga tatgccgcag
     6421 gaaatcctgt acggcttggc aaaggcggca ggcgcgtgcg gaatcgtcgg aacctgtttt
     6481 gaaaagtcgg gcggatagca ggcataccgg cagattcaaa ggcggagcag acattcggac
     6541 aacatgccgt ctgccgcccg aatagcggtg ccgtctgaag cgcacaagac ggtttcagac
     6601 ggcatataa
//
LOCUS       DAWKST010000114         6483 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.114, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000114 DAWKST010000000
VERSION     DAWKST010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6483
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.114"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            13..339
                     /locus_tag="WJL87_001526"
     CDS             13..339
                     /locus_tag="WJL87_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ7799942.1"
                     /translation="MGEKIKDGKNQKTVLSREEEQKICNTFTHKQAVEDFSVVIGYDE
                     IKAKNHSLSAGQYFEVKIDYVDISADEFAQKMAGFSADLDKLFAESAELEKEIKDRLA
                     MLKFNS"
     gene            350..487
                     /locus_tag="WJL87_001527"
     CDS             350..487
                     /locus_tag="WJL87_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ7799943.1"
                     /translation="MKPVKLKMPSESLSDGILFGLENTYAVLSLKERIQKTKATGIYR
                     K"
     gene            complement(647..1984)
                     /gene="ribB"
                     /locus_tag="WJL87_001528"
     CDS             complement(647..1984)
                     /gene="ribB"
                     /locus_tag="WJL87_001528"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ7799944.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPADIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHNLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPAADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERVQQAESGVVILLHRTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILVGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(2209..3221)
                     /locus_tag="WJL87_001529"
                     /pseudo
     CDS             complement(2209..3221)
                     /locus_tag="WJL87_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(3389..3982)
                     /gene="ribA"
                     /locus_tag="WJL87_001530"
     CDS             complement(3389..3982)
                     /gene="ribA"
                     /locus_tag="WJL87_001530"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ7799945.1"
                     /translation="MSEMLNHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(3975..4652)
                     /locus_tag="WJL87_001531"
     CDS             complement(3975..4652)
                     /locus_tag="WJL87_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799946.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGTASIDRSV
                     LWRSDEGWKLADYLSCDNVREDALKRLFMALDSVFSRSTGVQSAAVYALMPSENQAFQ
                     LICLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECADEAAQAEWVALALALSEPLKLLLGMSAG
                     KDGSEDV"
     gene            4806..4890
                     /locus_tag="WJL87_001532"
     tRNA            4806..4890
                     /locus_tag="WJL87_001532"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4840..4842,aa:Leu,seq:tag)
     gene            5411..6445
                     /gene="purM"
                     /locus_tag="WJL87_001533"
     CDS             5411..6445
                     /gene="purM"
                     /locus_tag="WJL87_001533"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ7799947.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGELDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQDLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
BASE COUNT         1605 a         1591 c         1692 g         1595 t
ORIGIN      
        1 gacgcatcgg gcttgggcga aaaaatcaaa gacggcaaaa accaaaaaac cgtactttct
       61 cgcgaagaag aacaaaaaat ctgcaatact ttcacgcaca aacaagcagt ggaagatttc
      121 agcgtggtga tcggctacga tgaaatcaaa gcgaagaatc acagcttgtc ggcggggcag
      181 tatttcgagg taaagattga ttatgtggat atttccgccg acgaatttgc gcaaaaaatg
      241 gcgggatttt cagcggattt ggataaactt ttcgccgaat ctgccgaatt ggaaaaagag
      301 attaaggata gattggcgat gttgaaattc aattcataaa taccgatgaa tgaaaccggt
      361 caaacttaaa atgccgtctg aaagcctttc agacggcatt ttgtttggtt tggaaaatac
      421 ttatgccgtt ctttccctga aagagagaat ccaaaaaacc aaagccacag gaatttatcg
      481 gaaatgacaa aaacccgacg aaccggattc ccgcctgcgc gggaatgacg aattagaagt
      541 tacccgaaat ttgcaaaaac aaaatccaac ccaacagacc gggttttcgt ttgcacggaa
      601 ataatgcaat aaataaagca aatataaagt atttgaattt actatattat ttttccgctt
      661 cttcaaagcc gacgacttcc aaaccgaagc cggtcagacc ggtgaaagat gagggctgcc
      721 cgaggacgcg cagttttttg acgttgaggc cgacgaggat ttgtgcgccg atgccgtagc
      781 ttttgctgtc ccatttgtag gcttggtttg cgcctttggg tagggtgcgg tcgagcaggg
      841 atgcgccgtc ttcggtgcgg tgcagaagga tgacgacgcc gctttctgcc tgttggacgc
      901 gctcgagtgc tttgggcaat gaccatgaat ggcgcgggtt ggcttgaatg aagtccatca
      961 cactgaaggg ttcgtggacg cggacgaggg tttcggtgtc ggcggcgggc gtgcctttga
     1021 cgagggcgag gtgggtttcg ccggagagtt tgtcgacgta aacgtgttgt tggaactcgc
     1081 cccacggggt ttgtacaggc gcattgccca tgtcttcgag caggctttcg gtacggctgc
     1141 ggtattcgat gaggtcggca atggtgccga ttttaaggtt gtgttcttcg gcaaacttca
     1201 tcagctcggg catacgcgcc atggtgccgt cgtcgttgat gatttcacag atgacggcgg
     1261 caggaatcag cccgttcatt tgcgccaagt cgacgccggc ttcggtgtgt ccggcgcgga
     1321 cgagtacgcc gcctttttgg gcgcgaagcg ggaagatatg accgggttgg acgatgtctg
     1381 cgggtttggc ggtcggggaa acggcggttt gaatagtcag ggcgcggtcg gcggcggaaa
     1441 tgccggtggt aatgccgtgt gcggcttcga tggagacggt aaagttggtg ccgtattgcg
     1501 cgccgttttt ttgggtcatc atcggcagcc cgagtttttc gaccatttcg ccgtccatcg
     1561 gcaggcagac caagccgcgc gcgtgtttga tcatgaagtt gatggcttcg ggcgtgacga
     1621 attgcgccgc catcagcagg tcgccttcgt tttctcggtc ttcggcatcg gtgatgatga
     1681 ccattttgcc ggctttgatg tcggcaagga tttcggggat gggggaaata tgggacatgg
     1741 tgtgttttcc tgtgtgttcg ggcgggcggg cggtgtgttc ggtatcgagc cagaaagcgg
     1801 gcatttttgc cgcctgttcg attttacggg cttttttctc gccgaaggat ttgtttttca
     1861 aaagggcgga gagttcgcct gtgttgaggg caacggcttc ggcaaaacgg gtttgcaaac
     1921 cgccgtagtg ttctgctatc cattgccgca ggttgcggcg gcgcagaccg gctgtgtcgg
     1981 tcatcgtttc atcctttgta gaaatcggat gtagcggatt gtaatcgcgt atttaccaaa
     2041 aagcaaatct gtttttttcg ccaaaccgaa ttatttgctt tttggtaaac agatgccgtc
     2101 tgaagccgga ttcgtggctt cagacggcat tgccgcaatc cttgccggcc gggggctgtt
     2161 tgtcccaact tcccgcgcac ttctgatttg gggtttgccg gaagcttgtt ccgaaaaaaa
     2221 tttctgataa acggccttga ttgccgggta cagcataggc ggtacgagca ggcgcaatat
     2281 cggcaatcct atgatgaatg cggtcttccc gataaaaacc ggacggattg ccttcgcgcc
     2341 gaatatcttt ctgcattttt ctttaaatga aaaatatttc cggtagatga ggataatgtt
     2401 tttggaaaaa taaagcctta ccaatataga cctcagatag ccgatattgt cgaagacaaa
     2461 tttttgcaaa ccgccgtaga tgggggaatt tttgttttcg actaaaaaat ccacaccccg
     2521 gtcgagatgc gtcagcacat cgctgaaatt tttgtatttg atgttgtggc tgatagagcc
     2581 gccgagaacg cggtactggt aaaaagggtt atcgtaaaag gcaaaagtct tgataaaacg
     2641 cgccaattgc aaactgtacg ggaaatcctc gtgaatgtat ccttttggga aaaacagatt
     2701 gtttttgatg attatttccc gcctgacgat ttttgtccac gcgttggcga tatagtaccg
     2761 cccctccacc agcgtttcaa aatggcggac aaaatcatta tccgaaaagt ccgccccttt
     2821 ggggatgtcg cggtaattga aggacgaggg atgcaggatc aaatcaacct ttttgtctgc
     2881 aagttgttgt aaatcaaaga gaaccccccc ccccgcgcgt tttttgaacg attggtgtcg
     2941 gcccaatagt cgtcgctgtc caaaaagatt aggtaatcgc cttttgccgc ccggataccg
     3001 gcgttgcggg catccgacag cccgccgttt tcttgatgaa tcacttttat atgcggatat
     3061 ttgcctgcat attcgtcgca aatcttcccg cagccgtccg gcgaaccgtc atcgaccaaa
     3121 atcatttcat aatcggcaaa attttcggca agcacggaat ccacgcagca gcggaggtat
     3181 ttttccacat tgtaaatagg gacgatgatg gagaaaatca taaatatcaa tccattatat
     3241 taagatgttt gcgcgtatgc ctcaaacccg cacccgcaat gcgtttgcat ccgcaccctg
     3301 taactttata aaacttattg ctttatcaag gtatggaaac ctgtttcccg aaaggcggcg
     3361 caggatgccc gttccctgca tctttgcctt attccgacat cagatgcccc agcttgtctg
     3421 ctttggtttg gaggtagcgt ttgttttcaa ggttttcccc gacgtgcagg gggatgcgtt
     3481 cgaccacgtt aatccccgca tctttcaggg tttggatttt ttcggggttg ttggtcagca
     3541 gtttgaccga gcggatgccc agatattcgt agatagattg cgccaaacgg aaatcgcggg
     3601 catcgacggg cagcccgagt gccaaattgg cttcaaccgt atccatacct tggtcttgca
     3661 gatgataggc gcggattttg ttaatcagcc cgatgccgcg tccttcctga cgcagataga
     3721 cgatgatgcc gcgcccctct gtctgtaccg ccctcatggc cgcttcaagt tgaggtccgc
     3781 agtcgcattt tctcgagaac agcgcatcgc ccgtcaggca ttcggagtgg atgcgcgtca
     3841 gaaccggatt gccgtctgaa aaattaccga cggtcagcgc gacgtgttcc tgcccgtttg
     3901 cctcttcaaa gccgtgcatc gtaaatacgc cccattcggt cggcaggcgg caggatgcca
     3961 catggttcaa catttcagac atcttcactc ccatcttttc cggcagacat acccaacagc
     4021 agtttcaaag gttcggacaa agccaaggca agagccaccc attccgcctg cgctgcctca
     4081 tccgcacact ctgcgcattc aaattccaca tgaaccacgc ccaacacacc gccactttcc
     4141 gtgcagaccg gaatggaaat ttgcgccgcc gaagcatgat tgcgttctcc cgaaagctcc
     4201 cccaaatcca accaacggcg tacatccgag gcaacattca tccaaccgct ttgcgccgaa
     4261 cggcaagcca gcgaaacctt gcctgccgct tcatccaaat cccacaggtt ttccaaaacc
     4321 tcgccctgtc gggacaggca tatcagttgg aaagcctggt tttcagatgg catcaaggca
     4381 tagaccgccg cactctgcac gcctgtcgag cgcgaaaaca ccgaatccaa agccatgaaa
     4441 agccgtttca gtgcatcttc gcggacattg tcgcacgaca ggtaatcggc aagtttccaa
     4501 ccctcatcac tgcgccacaa aaccgaacgg tctatcgaag ccgtccccat atccattacc
     4561 gtctgcgccg tcagatacgc cgcccgaacc tcgtcaagcg gcagcttcaa accctgagtc
     4621 agcagaaaat ccttaatcaa agcagcaggc atacccaaat tccgtcaata aaaacaaaaa
     4681 accgcccgat tccgatgtca ggcggacgaa aaacagattt taccgcccca aaccccaagc
     4741 catcaacagc cccgccgaaa accttgcaca aaaaaacaaa atctgtataa tcgtccaatt
     4801 cccatgcgga cgtggcgaaa ttggtagacg caccagattt aggttctggc gccgagaggt
     4861 gtgagagttc gagtctctcc gtccgcacca ataatgataa ataaattata tagttaatgc
     4921 ttatcgggac aaattagtgc cacaaaacat aagtcgtctg cttttcagcg ggcggctttt
     4981 ttatctgcct atatgtttca acacgcagaa cggtacataa agtgccgcca tttgttccgc
     5041 accgaatcgg aaacgggtgc ttccgcccgc aatcagattc accgccccta atgccggggt
     5101 ttcaaccgga aagaagaaaa tattgatttt gatgatggca tcaaaaaacc ccttccgctc
     5161 gtctgccaaa aggaatggtt aagaaaagca atgcgtcttt atgatggaac gcgatatata
     5221 aggaattgag ggaaagggca ggcgggtttc tgcccgttaa tcctgcggac ggtcccgcca
     5281 aacccgacac gcgcgggcat atggcggact gtttacaata ttgtctaaaa atctgaacct
     5341 gcaaacgtga aataaggtag aatacgcccc gttattttac cgtccaatcc ccatttacca
     5401 aggaaaaacg atgagtactt cattgagtta ccgcgatgca ggtgtcgata tcgacgcagg
     5461 cgaccaactg gtcgaaaaca tcaaaccgtt tgccaaacgc accatgcgtc cggaagtatt
     5521 gggggatttg ggcggttttg gcgcattggt cgaaatcggc aagaaatata aaaatcccgt
     5581 attggtcagc ggtacggacg gcgtgggtac caagctcaaa cttgcctttg attgggataa
     5641 acacgatacg gtgggcatcg accttgttgc aatgagtgtc aacgatattt tggttcaagg
     5701 agcagaacct ctgtttttct tggattattt cgcctgcggc gaattggatg ttccgcgcgc
     5761 aaccgatgtc atcaaaggca ttgcacaagg ttgcgaagaa tccggctgcg ccctgattgg
     5821 cggggaaact gccgaaatgc cgggtatgta tcccgtcgga gaatacgatt tggcgggttt
     5881 tgccgtcggc gtagtggaaa aagagaatgt cattaccggc cgcagcatcg gcgcgggcga
     5941 tgtggtattg ggtttggctt ccaacggcgc acattcaaac ggctattccc ttatccgtaa
     6001 aatcatcgaa cgcgacaatc ccgatttgga tgccgagttt gataatggca aaaccttgcg
     6061 tgaggctgtt attgcgccga cccgtctgta tgtgaaaccg attcttgccg ctttggaaaa
     6121 atttaccatt aaaggtatgg cacacattac cggcggcggc attaccgaaa acgtgccgcg
     6181 cgtgttgcct gaaaacacgg ttgcacaaat cgatgctaaa tcgtgggaat tgcccaagct
     6241 cttccaatgg cttcaaaagg cgggcaatgt ggaaacccaa gaaatgtacc gaacctttaa
     6301 ctgcggcatc ggcatggtcg ttattgttgc tgccgaagat gccgatgcgg ttcaggatct
     6361 cttgggtgaa caaggtgaaa cagtttaccg tttaggtttg atccgtgagc gtcagggaga
     6421 cgagcatcaa acccaggttg cctgattgct tctatagcga aatgccgtct caaacatggg
     6481 ttt
//
LOCUS       DAWKST010000115         6417 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.115, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000115 DAWKST010000000
VERSION     DAWKST010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.115"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            175..1233
                     /gene="alr"
                     /locus_tag="WJL87_001534"
     CDS             175..1233
                     /gene="alr"
                     /locus_tag="WJL87_001534"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ7799948.1"
                     /translation="MRPLNVQIRLGNLRHNYQILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLIRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(1297..2652)
                     /locus_tag="WJL87_001535"
     CDS             complement(1297..2652)
                     /locus_tag="WJL87_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ7799949.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAASKADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINVGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(2668..2940)
                     /locus_tag="WJL87_001536"
     CDS             complement(2668..2940)
                     /locus_tag="WJL87_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ7799950.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(3066..3803)
                     /locus_tag="WJL87_001537"
     CDS             complement(3066..3803)
                     /locus_tag="WJL87_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ7799951.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGNIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHQRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(4084..4983)
                     /gene="prmB"
                     /locus_tag="WJL87_001538"
     CDS             complement(4084..4983)
                     /gene="prmB"
                     /locus_tag="WJL87_001538"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ7799952.1"
                     /translation="MFNQAAQELTTIRDILRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGNGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            5128..5722
                     /locus_tag="WJL87_001539"
                     /pseudo
     CDS             5128..5722
                     /locus_tag="WJL87_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5834..>6417
                     /locus_tag="WJL87_001540"
     CDS             5834..>6417
                     /locus_tag="WJL87_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEZ7799953.1"
                     /translation="MTKLYAEIAKMETQDDDTVKVWGYASSEEIDSDGEVIAAAAMKA
                     AIPDYMKFGAGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVSKVKTGVYKGFS
                     IGGSVTARDDLNKSQITGLKLTEISLVERPANPDAVSTCFKADKGAETVNNDTEHNAT
                     YFSHFPSKQKSTDGGRCPFLYRFPYFLSAGRAGFA"
BASE COUNT         1718 a         1620 c         1500 g         1579 t
ORIGIN      
        1 tttgggaaag cgattatatc aggaaaagca aaccgccttc ctacctgaaa actgctgctt
       61 cggcttgaag acacaagggt ctttaatatt ttaaaagcct tgccgttgga ttataattcc
      121 ctaattgatt tcttaatttt gctaataaac acttgtttgg taaggaatga atttatgcgt
      181 cctttgaacg tgcagatcag gttgggcaac cttaggcaca attatcagat tttaaaggaa
      241 atgcacggag gcaagctgtt ggcggtagtg aaggccgacg catacggaca cggtgcggtc
      301 agatgtgctt tcgcgctggc agacttggca gacggctttg ccgtggcgac aatcgatgaa
      361 ggaatcaggc tgcgggagag cggcattacc catccgattg tccttttgga aggcgtattt
      421 gaagcatcag agtacgaagc ggtcgaacaa tactcgcttt ggccggcagt cggaaaccaa
      481 tggcagcttg aggctttgct gatccgccat tggaaaaaac ccgtcaaagt ctggttgaaa
      541 atggattcgg ggatgcaccg taccggtttt ttccctcatg attacgcttc ggcatatgcg
      601 gcattgaaac agtcggaata tgtggacagt attgtcaaat tctcgcattt ctcctgtgcg
      661 gacgaacccg aaagcggtat gacggaaata cagatggaag cattcgattt gggtacggaa
      721 gggctggaag gcgaagaaag ccttgccaat tcggcggcta ttttgaatgt tcccgaagca
      781 cgcagggatt gggggcgcgc gggcttggcg ttatacggca tttccccgtt cggaggcagc
      841 gatgacaggc tgaaacccgt gatgaggctt tcaacccgta tttttggcga acgcgtttta
      901 cagccgcact cccctatcgg ttatggcgca acattttata ccagcaaatc tacgcgcgtc
      961 ggtctgattg cctgcggtta tgcggacggt tatccgcgcc gcgccccaag caattccccc
     1021 gtcgctgtcg acggcaaatt gacccgggtc atcggcaggg tctctatgga tatgatgacc
     1081 atcgagctgg acgcttcgca agaaggtttg ggacacgagg tcgaactgtg gggcgatacg
     1141 gtcaacatca atactgttgc cgaagcggcc ggaaccatcc cttacgaatt gatgtgcaat
     1201 atcaaacgtg caaaattcac ttatatcgag taatcaagtc caaacaaaaa tgccgtctga
     1261 agcctttcag acggcatttc cccatcaaaa ccgcaatcag tttttcatcg attgaaccgg
     1321 tgcgggaatc ctgccgcccc ggttgacgaa cacttcgcat gagccttctt ttaccggcat
     1381 tacaggcgcg tagcccaaca ggccgccgaa ctcgacgctg tcgccgacgg tttttccggt
     1441 taccggaata atgcgcacgg cagtggtttt gctgttgatc atgccgatgg cggcttcgtc
     1501 ggcgatgatg ccggaaatgg tgtgtgcggg cgtatcgcca gggacggcaa tcatgtccag
     1561 cccgaccgag cagacggcgg tcatcgcttc gagtttatcc aacgtcagca cgcctgcttc
     1621 ggcggcggca atcatacctt cgtcttcgga aacggggata aacgcgccac tcaaaccgcc
     1681 aaccgcgctc gaagccatca tgccgccctt tttcacggca tcgttcagca atgccaaagc
     1741 tgctgttgtg ccgtgcgtac cgcagacgct caaacccatt tcttcaagaa tgcgcgccac
     1801 cgagtcgccg acggcagggg tcggtgccag cgacaagtcg agaataccaa acgggatatt
     1861 cagcattttt gaggcttcgc ggccgatgag ttcgcccacg cgggtaattt tgaaggcggt
     1921 tttcttcaca acttcggcaa cttcggtcaa tgtcgttgca tccgaatttt ccaacgcggc
     1981 ttttacgaca cctgggccgg atacgccgac attaatcaca gcatccgctt cgcccgatcc
     2041 gtgaaacgca cccgccataa acggattgtc ttccaccgca ttgcagaaca cgacgatttt
     2101 cgcgcagccg aaaccttcgg gcgtaatttc agccgtgcgt ttgatggttt cgcctgccag
     2161 cttgaccgca tccatattga taccggcgcg cgtactgccg acattgatgg agctgcacac
     2221 gatgtcggtg gtcttcatcg cttcgggaat ggaacggatt aacacctcgt ctgaaggcga
     2281 catgcctttt tgcaccaacg cggaaaagcc gccgataaag gacacgccga tggctttggc
     2341 agccttatcc aaagtttgcg ccacgctgac gtaagaatcg gctttgctcg ccgccgcgat
     2401 ttgggcaatc ggcgtaacgg aaatgcgctg attcacaatc ggcacgccgt atttggcaga
     2461 cagatatttt gccgtcgcca ccaagtcttt gccgaccgtg gtgattttgt tgtaaatgtt
     2521 ttggtttaac acgtcgatgt cggtgctgat gcagtcgtgc aaatcaatgc cgatggtaat
     2581 ggtgcgcacg tcaaaattct ggtcggccac cattttgacg gtttctaaaa tctcgccgga
     2641 ttgaatactc atggctctcc ctccgactta aatacggtgc atggcttgga aaatttcttc
     2701 gttttgcata cggatatcaa gcgcgagttt tttgctctct tccgcaaaca aatccaaaac
     2761 ctcctgccga gatttggggc atttcgaggt atccaccaag ataatcatgg taaaaaaatc
     2821 gtccatcagc tgttggctga tattaaggat gttgattcgg ttttccgcca aaattttgga
     2881 aacatcgtac acgatgccga cgcggtcttt gccgatgacg gtgatgactg aattgttcac
     2941 aggcttactc cttgcagata tccgttaaag tccgaaatta taccaccgtt ggattttgaa
     3001 gaaatattgt caacaatata tacatacaaa atgccgtctg aaactatttc agacagcatc
     3061 aagattcagg gttcgattaa ataaccatcc ttatcccact gggttttcct gaccaacttg
     3121 tcatcctgat aaacagcctc gctcttttta gaaccatctt cataccactc caaaaccacc
     3181 ccgttgcgtt gatggtggcg gatagacagt tccgagagta atcggccgct ttcatcccaa
     3241 gtcagaattt tggcaggctc atcgttgacc ataaccattt ccgttttgat attgccgtcg
     3301 gcataccatt gcttccatac gccgtttgcc ttattctgtt taaactggat ttcgctttcc
     3361 ttgccgccgt tacggtaata gcggtacccc gtaccttcac tcaaaccatt tttataaggc
     3421 ataacggcag attttttacc gttcggatac cagttgaccc actccccgtc cggcttaccc
     3481 ttgctgaagc cccccgccat ttttttctga ccattaaaat gccacaaaat caacataccg
     3541 ttttgcaggg taggcacaaa agatttgatt tgcgttgaag caacgatata aggttcggaa
     3601 tatttcttca tcgacggata ataaaaatcc tgcgcgtgcg caataccctc cgccacacta
     3661 tattgcctga tataagcggc agaagacatc gtcgccgtca gtttcccgtt ctgattaaaa
     3721 taaacagaat aggtctgcgc cggcaaagcg gccgaaaaac ccaacaggac agttgaaaat
     3781 acaatccgag ataatttttt cattgcaata gcgatataaa aacaaggctg tgttttagta
     3841 atcttttgat ttcaattatt tgcaagggaa aagacaatta ttttccggtt aggaataaac
     3901 ctattctatt gaatatattg aagccaagta cgcctatcaa cactatatta aaacactgcc
     3961 aaaaacaatt aacttataaa caatatggta aggatttctc tgccaagcat caaacccgag
     4021 acaacgtatc gtaaaaatgc cgtctgaaaa caaatcgtct tcagacggca tttccccttc
     4081 aactcactct tcacccaata actgctcgcg cgtcaagagg aaaacaaaac cgtcgccccc
     4141 gctggtttcc aaccaagtaa aaggcaactc cggatacgct gcttccaata catccctgtt
     4201 atgcccgatt tccaccagca atacaccttt gggattcaga aactttgccg cattcagaag
     4261 aatctgcctg gtggcatcca acccgtccgc cccgctgccc aatgccaatt ccggttcgtg
     4321 caaatactct tcaggcaata actcaaccga ttccgcatcc acataaggcg gattggaaac
     4381 aatcaaatca taagtgcctt ccaatccttc aaacaaatcc gtatgaataa gccggatgcg
     4441 ttcttccaaa ccataatctt cgacattaat ccctgccact tccaaagcat ccaagctcac
     4501 atcaaccgca tcaatttggg catcaggata atgatgcgcc atctgaatgg caaggcaacc
     4561 gcttccggtg caaagatcca aagcattatg caccagctca tcgtattcta tccaaggacg
     4621 aagtccgtta cccaacaatt cataaataaa agaacgaggt atgattacgc gctcatccac
     4681 atagaaatca aactctccct gccatgcctg gtgtgtcaaa taagcggctg gaatgtgttc
     4741 gacagcacga cgctcaataa ccgccagcac ttcctctttt tcagcttcca agagttttgc
     4801 atcaagatat ggggcaagca tatccaaagg caaattcaaa gtatgcagaa tcaaataagc
     4861 cgcttcatca tgcgcattat ctgttccatg accaaaaaag agccctgcct cattaaaacg
     4921 gctgactgca aaacgtaaaa tatcgcggat agtcgtcaat tcttgtgctg cctgattaaa
     4981 cataatatga accattctgc gtatagatac ttttaattat aacagaaaca acaagcaaac
     5041 cttttcatat cgccaaataa ccacccaatc tacccataca actacataaa tgcccgcgcg
     5101 aaaaccatcg cccgaacgga aacgacaatg gccgacggta tgggcaatct gattggctgg
     5161 ggaaaaaacg gggcttgttg tcggtaagca gtggataacc gcaaaagacg acaaggtgtc
     5221 cgatgtctgc aatgccaacg gcgagatggg cgtaatcggg ctttacgagc ctttctcaca
     5281 cggcgcattg acgatacccg gtcatccgaa ctgccgatgc gaggttgttt ccgtatcggg
     5341 tggcgaattg ggggaatttg ccgaaaaaaa ggagcttcgt aaagcggcta tgcagtatgc
     5401 gcgggataac tttatcggca aaagctatgt caatgaaaac agcgggcatg aactgaaggt
     5461 aacttggcaa ggtgtgaaac acgctgcgtc aaaggcaaat caggcggaat tatccatcat
     5521 gacaaaactt gatgacttat tgcgctacgc aaaatatgag ggttcttatt cggataggaa
     5581 aggtcatcct aatattattg cagcacataa gtatcgtgcc gttgccaagg ttgggaataa
     5641 gtctttaaat atcggtgtga ttgtaaggga atttccagac gaccataaac attacgacca
     5701 tttcatcttg aaggatgaat aaagcccttt tgcagtgtcg ttctggagcg gatagcgtta
     5761 aggcaagtac acttccagcc ttgaaaaagg gctttaaatt cagcatgcca tttatacagg
     5821 caggagtaaa cccatgacaa agttatacgc agaaatcgcc aagatggaga cgcaggacga
     5881 cgacacggtc aaggtttggg gttacgcttc aagcgaggaa atcgattcgg acggcgaagt
     5941 catcgcggcg gcagctatga aggcggcgat tcccgattat atgaagtttg gcgcggggcg
     6001 cgagatgcac ggctcaaacg ctgcgggaac ggcaattgaa atcaacgttg aagacgacgg
     6061 cataaccttt ttcggtgctc atatcatcga ccccgttgtc gtgtcgaaag tcaaaacagg
     6121 cgtttacaaa ggcttttcca tcggcggcag cgttaccgcc cgcgatgatt tgaacaagtc
     6181 gcaaatcacg ggcttgaagc tgacggaaat cagccttgtc gagcgccctg ccaatcccga
     6241 tgcggtgtct acctgcttta aggcggacaa aggtgcggaa acggtaaaca acgatacaga
     6301 acataatgct acatatttta gccatttccc ttccaaacaa aaaagcaccg acggcggccg
     6361 atgccccttc ctttacaggt tcccctattt tttatccgcg ggcagagccg gttttgc
//
LOCUS       DAWKST010000116         6333 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.116, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000116 DAWKST010000000
VERSION     DAWKST010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.116"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            47..1210
                     /locus_tag="WJL87_001541"
     CDS             47..1210
                     /locus_tag="WJL87_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ7799954.1"
                     /translation="MHINFSMEYKEFNENYLYKKPFIFKKALDVSSISWKEINELYQR
                     ADPTDWQFKFRKGEIIPKEAYVESFNDVGRIRHRFNKTAVYQYLQDGATMVYNRIDNE
                     PFVDSIAKQIAQFAQAQTVVSGYLAFGSSPSYRNHWDTRDVFAVQLIGKKHWTVSAPN
                     FDMPLYMQQAKDMPHITPPTTVDMEVILEAGDILYIPRGWWHNPMPMNCETFHLAIGT
                     FPPNGYNYMEWLMKKIPDIQSIRQNFIDWEHDQKNIDNAAQAVTEMMKNQENYQAFIQ
                     DFLGNQRVNTAFNMQIFGNLDNDRLPENSTIKLNSLDNRTIKQGYIIANGIKTNLDND
                     SQTILQWIADKHSVKLTQLYEFCQNQNINLEKVEKLVFDLTMIDVLECLTDER"
     gene            1217..1423
                     /locus_tag="WJL87_001542"
     CDS             1217..1423
                     /locus_tag="WJL87_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799955.1"
                     /translation="MFTFLNKNIFIKTIVLGCFYSFLFFSFFVSGKSFGYSFEIFNMD
                     TSALAFLSVYMGFFISVAVHIYDI"
     gene            1447..1782
                     /locus_tag="WJL87_001543"
     CDS             1447..1782
                     /locus_tag="WJL87_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ7799956.1"
                     /translation="MKELTINDLTLVSGGFEVGRDYIEIAPVVGTAGGVASSVRAASM
                     TGEAGAILTRSGVTGLRAGVVAIPLLAYNGTVWLNRNTKLQDNLGRLIDKVTGLDQSG
                     NDYGDGTSY"
     gene            1817..3922
                     /locus_tag="WJL87_001544"
     CDS             1817..3922
                     /locus_tag="WJL87_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEZ7799957.1"
                     /translation="MSIYLSRLSFGFSRRLLVVLQTEAAECGLACLVSVLGFHGFYTD
                     LRHLRARFSLSLKGATLADLVRFANSMNLTARAVRLDLDELVNLRLPCILHWDLNHFV
                     VLHEVHRNHIVIMNPASGWQKVRMEEVSRCFTGIALELFPNTQFEEKHETRKIRILPM
                     LRGVVGLKRSLFQLLLLAAVLQVFALASPFFMQWVIDHAIVSADRDLLLTLALGFGLL
                     MIVQQLVSLLQTWAGMYLSTSLNIQWKANVFRRLMDLPVSYFTKRHLGDVVSRFGSVD
                     SIQSTLTSTFFVLVLNSIMAVFTLGLMYLYSPSLTAVVLVVLLIYICIRWVAYYPLRH
                     ATEENIVHAAKQSSYFMETIRGIQTVKLFDKNAQRHAAWLNLFVDTINTGLTTQKLSA
                     MFGFANSLLFGIANILIVYFGAVSVLDGVFTVGALMALMAFMSYKSQFESKAGSLIDQ
                     FVQIKMLGLHAERLADIVLEKTENEQNNDFRLPKNLEHFDIKIENLSFRYAENEPYIL
                     QDFSLIIKQGTAIAFAGHSGCGKSTLIQILTGSLKPESGHVLLGENDIHAFPPAFIRK
                     WSASVMQDDVLFAGSIAENISFFDDTPNMEKIAFCAQMANIHHEIVAMPMAYETLIGD
                     MGSALSGGQKQRIVLARALYREPKILFLDEASSHLDINNERVINDNLRQLNITKIMVA
                     HRQETLNTADSVVYLDKVA"
     gene            3924..5186
                     /locus_tag="WJL87_001545"
     CDS             3924..5186
                     /locus_tag="WJL87_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ7799958.1"
                     /translation="MTLFRPEVFQAKKNRWTGQIVLVRPFSLQFLTFFAVSLAAILVA
                     FLIFGSYTNKTTVTGQLLPTTGVVRVYSQDMGVIAHQHVMNGDFVKKGDVLFTLSTSR
                     NDNNGSIQARLLAEAELKKSLSEQEIIMKKRVHAAEKTAQENTVHRFQNQMQHVRNQI
                     IMQEKRIAISEKMLEKQRYLAKMDAISELEKNSYEIALLELKAGLAAYQREADNLARE
                     ITVQQSNLKNLPEQQATEISQLERAVSVYQQEILDYQQRNEQTIRATISGYVSSINTE
                     IGQQVDTNKLLMSIVPKESELLANLYVPSRAIGFVKPNDKVILRYQAYPYQKFGHAEG
                     HVISIAQTALGAQEWTNLGNIFTQTAQVNEPVYLIKVKLDNQHIRIYGTEKKLQIGMI
                     LEADILHENKRLYEWILDPLYQVMGKIS"
     gene            complement(5456..6295)
                     /locus_tag="WJL87_001546"
     CDS             complement(5456..6295)
                     /locus_tag="WJL87_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799959.1"
                     /translation="MGNFAKVSNCEFWDKINYNRAAQAAEQRAKQAQETPKEPPKQEE
                     HFIAKLPNDKLMTIYRLFQKQLDDMRGLERDIERGDWYINARYRMNENRNKIRALEDD
                     NKRLHHDYWAVQDQGENRFFGMGKIGAKLVKENEKQQHINERHGANVREIRRLKTENE
                     KLEGQYSDKLAADVVKVRDRFNEELDNVRREIGKRPMEEFRNVFLQAAQEAQTTIGQK
                     AVQIELEKFPLNGSPSAKRIAFYFGAQRIREVEQKEREDQRKEQERQARRMERDDGHG
                     IGM"
BASE COUNT         1849 a         1164 c         1306 g         2014 t
ORIGIN      
        1 aagtaaactt aattttcatt attaataatg aaaataagga ttatttgtgc atattaactt
       61 ttcaatggaa tataaagaat ttaatgaaaa ttatttatat aaaaagccgt ttatttttaa
      121 aaaagcttta gacgtaagtt ctatctcatg gaaagaaatt aatgagttgt atcaacgtgc
      181 agaccctact gattggcaat ttaaatttcg caaaggggaa ataatcccca aagaagccta
      241 tgttgaatca ttcaatgatg tgggtagaat acgtcatcgg tttaataaaa cagccgtata
      301 tcagtatctg caagatggtg caacaatggt ttataaccgt attgataatg agccatttgt
      361 tgacagtatt gctaaacaaa ttgcccagtt tgctcaagca caaactgttg ttagtggtta
      421 tttggcgttt ggtagttctc catcatatcg taatcattgg gatactcgtg atgtgtttgc
      481 ggttcaattg attggcaaaa aacactggac agtttctgct ccaaattttg atatgccatt
      541 gtatatgcag caagcaaaag atatgccgca cattactccg cctacaacag tagatatgga
      601 agtcatttta gaagcaggag atattttata tatcccgcgt ggttggtggc ataatcctat
      661 gcctatgaat tgtgaaacat tccatttagc tattggaact tttccgccaa atggttataa
      721 ttatatggaa tggttgatga aaaaaatacc tgatatccag agtattcgtc aaaatttcat
      781 agactgggaa cacgaccaaa aaaatataga taatgcggct caggcagtta ctgaaatgat
      841 gaagaaccaa gaaaattatc aagcattcat acaagatttt ttgggaaatc aacgcgtaaa
      901 tactgcattt aatatgcaaa tttttggaaa tctggataat gacagattac ctgaaaatag
      961 tacaattaaa ctcaattcgt tggataaccg tacaattaaa caaggatata tcatagcgaa
     1021 tggcatcaaa accaacttgg acaatgatag ccaaacgatt ttgcaatgga ttgctgacaa
     1081 acatagtgtt aagttaactc aactgtatga attttgccaa aatcaaaaca ttaatttgga
     1141 aaaagtggaa aaattagtgt ttgatttgac aatgatcgat gtattggaat gtttaactga
     1201 tgaaagataa attattatgt ttactttttt aaataaaaat atatttatca aaacaattgt
     1261 acttggctgt ttttattcct ttttattttt tagttttttt gtatctggta agtcatttgg
     1321 ttattcattt gaaattttta atatggatac aagtgcgctt gcttttttaa gtgtatatat
     1381 gggctttttt ataagcgtgg ctgtgcatat ttacgatatt taatacaatt gaaaggataa
     1441 ataattatga aagaattaac tattaatgat ttaaccctag tttcgggtgg atttgaagtt
     1501 ggtcgtgatt atattgaaat agctccagtt gtaggtacag caggtggcgt tgcctcttct
     1561 gtgagagcag cttcaatgac tggagaagct ggagcaattc ttactcgttc aggagtaaca
     1621 ggattacgtg ctggagtggt agctattcca ttgttagcct acaatggtac tgtatggctt
     1681 aatagaaata ccaaattaca agataattta ggtagattaa tagataaagt aactggcctg
     1741 gatcaatcag gcaatgatta tggcgatggg acaagttatt aatctcataa tactttaaaa
     1801 tcaaaaaaga acaccaatgt caatatatct atctcgccta tcattcggct tttctcgccg
     1861 tttactagtt gtcttgcaaa cggaagcagc agaatgtgga ttggcgtgtt tggtatcggt
     1921 attggggttt catggttttt atacggattt gcgccattta agggcgcgtt tttctttgtc
     1981 tcttaaaggc gcaacattgg cagatttggt acggtttgct aatagcatga atttaactgc
     2041 tcgagctgtt cggttggatt tagatgaatt ggtaaatttg cgcttaccct gtattttaca
     2101 ttgggattta aatcattttg tggtgttgca tgaagttcat cgtaatcaca ttgtgattat
     2161 gaaccctgct tcgggttggc aaaaagtgcg tatggaagaa gtctcgcgct gttttacagg
     2221 catagcgttg gagctattcc ctaacacgca atttgaagag aaacacgaaa cgcgtaaaat
     2281 tcgcatacta ccgatgttgc gcggtgtggt tggattaaaa aggtctttat ttcagcttct
     2341 gctgctggcg gcagtattac aggtgtttgc tttggcaagc ccttttttta tgcaatgggt
     2401 aatagatcat gccattgtat cggcagaccg tgatttattg ctaacattgg cattgggttt
     2461 tggtttgttg atgattgtgc agcagttggt gtcattgttg caaacttggg caggtatgta
     2521 tttgtctact tcattgaata tccaatggaa agccaacgta ttccgccgct taatggattt
     2581 acctgtttct tatttcacta aacgacactt gggcgatgtg gtttcacgtt ttggctcggt
     2641 ggatagtatt caaagtacgc tcacttctac tttctttgtg ctggtactca acagcattat
     2701 ggcagtattt acattagggt taatgtattt atacagccct agtttaacgg ctgttgtatt
     2761 ggttgtttta ctgatttata tctgtattcg ttgggtggca tattatccgt tacgccatgc
     2821 aaccgaagaa aatattgttc atgctgccaa gcaaagcagt tatttcatgg aaaccatacg
     2881 cggtattcaa accgttaaat tatttgataa aaatgcacaa cgtcatgcgg cttggctgaa
     2941 cttatttgtt gatacaatca acacaggttt aacgacacaa aaactttccg caatgttcgg
     3001 ttttgcgaac agccttttgt ttggtatcgc taatatttta attgtgtatt ttggtgcggt
     3061 ttctgtgttg gacggtgttt ttaccgttgg ggcgttaatg gcgttaatgg cgtttatgtc
     3121 ctacaaaagt cagtttgaga gtaaagcagg ttcattgatt gaccagtttg tacaaatcaa
     3181 aatgctagga ttgcacgcgg aacgcttggc ggatattgtg ctagaaaaaa cggaaaacga
     3241 gcagaacaac gatttcaggc tgcctaaaaa tttagaacac tttgatatta aaatagaaaa
     3301 tctatcattt cgctatgctg aaaatgaacc atacattttg caagatttca gtttaattat
     3361 caaacaagga acagccattg cttttgctgg acattcaggc tgtgggaaat ctacgctcat
     3421 tcaaattctg acaggcagtc tgaaacctga aagcggtcac gtgttactag gcgagaatga
     3481 tattcatgcg tttccacctg catttatccg caaatggagc gcaagtgtaa tgcaagatga
     3541 tgtgttattt gctggctcaa tcgcagaaaa catcagtttt tttgatgata cgcccaatat
     3601 ggaaaaaatc gcattttgcg ctcaaatggc gaatattcat catgaaattg tcgctatgcc
     3661 tatggcgtat gaaaccttaa ttggtgacat gggaagcgca ttgagcggtg ggcaaaagca
     3721 gcgtattgtt ctagctcgtg ctttatatcg tgaacccaaa attttgtttt tagacgaagc
     3781 cagtagccat ttggacatca acaatgaacg tgtgattaac gataatctac gccaactcaa
     3841 cattaccaaa atcatggtcg cgcatcgtca ggaaacttta aatactgctg atagcgtagt
     3901 ttatttagac aaggtagcct gaaatgacat tatttcgccc cgaagtattc caagctaaaa
     3961 aaaatcgctg gacagggcaa attgttttag tgcgtccttt ttccttgcag tttttaacat
     4021 ttttcgcggt ctcattagcg gctattttag ttgctttttt aatttttggg agctatacca
     4081 ataaaaccac cgtaacagga caacttttgc cgacaacagg ggtagtacgc gtatattcgc
     4141 aggatatggg ggttattgct catcaacacg ttatgaatgg cgattttgtg aaaaaaggcg
     4201 atgtgttatt tacattatct acttcacgaa atgacaataa tggcagcata caagcacgat
     4261 tactcgccga agctgaatta aaaaaatcac tatcagaaca agaaattatt atgaaaaaac
     4321 gtgttcatgc agcagaaaaa accgcacaag aaaacacggt acaccgtttt cagaaccaaa
     4381 tgcaacacgt tagaaatcaa attatcatgc aagaaaaacg tattgcgatt tctgaaaaaa
     4441 tgttagaaaa acagcgttat ttagccaaaa tggacgcaat ttctgaatta gaaaaaaata
     4501 gttatgaaat tgctttattg gagctgaaag caggtttagc cgcttaccaa cgagaagcag
     4561 ataatcttgc tcgggaaata actgtacagc aaagcaatct aaaaaatctg cctgaacagc
     4621 aagctactga aatcagtcaa ttagaacgcg cagtatctgt ctatcaacaa gaaatattgg
     4681 attatcaaca acgtaacgaa caaactattc gcgcaaccat atcaggctat gtcagttcta
     4741 ttaacacgga aattggtcag caggtagata ctaacaaact attgatgagt atcgtgccga
     4801 aagaaagtga attactggct aatttatatg tccctagtcg cgcaatcggt tttgtaaaac
     4861 caaatgacaa ggtaattttg cgttatcaag catatcctta tcaaaaattc ggacacgcag
     4921 aaggacacgt catttctatt gctcaaacgg cactaggggc gcaagaatgg acaaatttag
     4981 gcaatatttt tactcaaaca gcacaagtga atgagcctgt ttatttaatc aaagtaaaac
     5041 tggataatca gcatattcgt atatatggca cggagaagaa attgcaaatt ggtatgatac
     5101 tagaagccga tattttgcat gagaataaac ggttatatga atggatactt gaccctttgt
     5161 atcaagtaat gggaaaaata tcatagctga attgctcaat acattggagc gtatgagtgc
     5221 ccctaattat caagcaaaac caatgtccga tgacttccaa tttgttgtta agcacaaata
     5281 cacagtgttt tatgtaataa tgaagacaga gaagtgatac aatttttcgc tccatttttg
     5341 cttcctgaat tgctgcaatt gtatctgcgt tgtatgcgag aagtaaatat tttcgatgca
     5401 tttgctgact ttgtaccata atacgtcttc caaccagtat gataggctgg tttttctaca
     5461 tgccgatgcc gtggccgtcg tcccgctcca tacgccgcgc ctgccgctcc tgctctttgc
     5521 gctggtcttc ccgctccttc tgctctactt cccttatccg ctgcgcgccg aaataaaacg
     5581 cgattcgctt ggccgaaggg ctgccgttca gggggaattt ttccagttcg atttgaaccg
     5641 ccttctgccc tattgtcgtc tgtgcttctt gggctgcctg caaaaatacg ttcctgaatt
     5701 cttccatcgg ccgtttgccg atttccctgc ggacattgtc cagttcttcg ttaaacctgt
     5761 cgcgtacttt gaccacgtcg gcggccaact tgtcgctata ctgcccttcc agtttttcgt
     5821 tttctgtttt cagccgccgg atttctcgca cgttcgctcc atgccgttcg ttgatgtgtt
     5881 gctgcttctc gttttcctta accagttttg cgccgatttt acccatgccg aaaaagcggt
     5941 tttcgccttg gtcttggact gcccaatagt cgtgatgcag ccgtttgttg tcgtcttcca
     6001 gcgcccttat tttgtttcgg ttttcgttca tgcggtatcg cgcattgatg taccaatctc
     6061 cgcgttctat atcgcgctcc aagccgcgca tgtcgtccag ttgtttttga aacagcctgt
     6121 atatcgtcat tagcttgtcg ttcggcagct tggcgataaa gtgctcttcc tgtttcggcg
     6181 gctctttcgg tgtttcctgc gcctgctttg cccgctgctc ggcggcttgg gcagcccgat
     6241 tataattaat tttatcccag aattcacaat ttgagacctt tgcaaaattc cccaaaatcc
     6301 cctaaattcc caccaagaca tttaggggat ttc
//
LOCUS       DAWKST010000117         6203 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.117, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000117 DAWKST010000000
VERSION     DAWKST010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.117"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            272..718
                     /gene="yciA"
                     /locus_tag="WJL87_001547"
     CDS             272..718
                     /gene="yciA"
                     /locus_tag="WJL87_001547"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ7799960.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIAQGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVEVWVKTL
                     MNDCVTEDRHLVTEAVFTYVAIDAEGNPRPIPKEGNPILAGLLPTP"
     gene            complement(769..1590)
                     /locus_tag="WJL87_001548"
     CDS             complement(769..1590)
                     /locus_tag="WJL87_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ7799961.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPNMVARGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDEKAAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1709..2167
                     /locus_tag="WJL87_001549"
     CDS             1709..2167
                     /locus_tag="WJL87_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ7799962.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAAAFADSSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2332..3447)
                     /gene="lst"
                     /locus_tag="WJL87_001550"
     CDS             complement(2332..3447)
                     /gene="lst"
                     /locus_tag="WJL87_001550"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ7799963.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGKRNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKTGDETGGTVRILLGSPDKEMKEISEKAAKDFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKN"
     gene            3548..3652
                     /locus_tag="WJL87_001551"
                     /pseudo
     CDS             3548..3652
                     /locus_tag="WJL87_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(3650..3820)
                     /locus_tag="WJL87_001552"
     CDS             complement(3650..3820)
                     /locus_tag="WJL87_001552"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799964.1"
                     /translation="MAIMAYFGGFVRALFDLGVFERGGVKGRGKWFSRVWRRSEVLCL
                     MCGGIFSVKLIL"
     gene            3880..6105
                     /locus_tag="WJL87_001553"
     CDS             3880..6105
                     /locus_tag="WJL87_001553"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ7799965.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTSDIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVIVPEATSVSIVFTDKQGNKKELREPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGDKLAAAGVNVNNGFGNLLANLDKLDADTRTAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAEGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNGKSPKRKAGELDNRGSHFYLTLYWAQELAEQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAVDIGGYYAANPEKAAQVMRPSVTFNQALNA
                     L"
BASE COUNT         1661 a         1813 c         1388 g         1341 t
ORIGIN      
        1 tgacgcggcg ggaatctatc ggaaatgact gaacccccga gattctagat tcccactttc
       61 gtgggaatga cggttcagtt gcttagggta ggatttggcg ggattggcgg actgaagccc
      121 actctacagc cccgccctac aatacgcctt gcgaatctgt cccacgcccg cccttgtatg
      181 tctcaaatgg gttaaactac gcacttcccg acttcccgtt tcgggcaggg gtgcaggttc
      241 gatttatctt tcaataaaac aaggaaactt tatgcagcac gaagaaggca accgccaacg
      301 ccctcaaggc gaactgctcc tgcgtaccgt cgctatgccg cgcgatacca atcccaacca
      361 agacattttc ggcggctgga ttatgtcgca gatggatttg ggcggcggca tattggcagc
      421 ggaaatcgca caaggacgca tcgttaccgt cgccgttcag gaaatgaact tcatccgccc
      481 ggtcaaggtc ggcaacgtcg tctgctgcta cgggcattgc gtccgcgtgg gcaatacttc
      541 cctccagctt aaagtcgaag tctgggtgaa aactttgatg aacgattgcg ttaccgaaga
      601 ccgccacctc gtaaccgaag ccgtgttcac ttatgtcgcc atcgatgcgg aaggcaatcc
      661 gcgcccgatt ccgaaagaag gcaaccccat attggcgggc ttattgccta ctccgtaaaa
      721 atgcccgtaa aaatgccgtc tgaaactgtc ttcagacggc attccgcatc atttgaacca
      781 agatctgatg cgggaaaaca gcgacatact ctgtttttca aagtcttccc gcacttccgg
      841 cacggctttt tttatcacct tcattcctgc aaaagtctgc gccacgggca taatttcaat
      901 cgtgttcaca ttcatatgcg ccggccggcg gtacagccac aatgcggttt ccgcaatatc
      961 ttcggggcgg ataaattcca caccctcata cacgcctgct gccttctcgt catcgccttt
     1021 gaagcgcacg ttggaaaact ccgtattgcc gcacaaaccc ggctcgatat tggtaacgcg
     1081 gatgttctta tccgccaact ccgcgcgcaa attcaggctg aactggcgca caaacgcctt
     1141 ggtcgccccg taaacgttgc tgccggcata agcataattg cctgcaatcg aacccaaatt
     1201 catcacataa ccgccgcccc gagccaccat attcggcaaa atcttgcgcg ttagaaacgt
     1261 caaacccaaa acattggttt gaatcattgt ttcccaatct tcaaaatccg ccttgtccgc
     1321 cgtgtccaga cccaaagcca gcccggcatt gttgatgagg cagtcgatgt cggaaaattc
     1381 atcagggatg ccgtttaagg cgttttccac cgactcgcgg cgcgacacgt ccatttccaa
     1441 agggtaaaac aaagcaccca attcatccgc caaggcctga agcctgtccg cacgacgcgc
     1501 cgcaccgata acgcggtatc ccgccccgac aaacgcgcgg cacatcgctt cgccgaatcc
     1561 tgccgaagca ccggtaatta aaacagccat tgtttttcct ttctttctcg tttcccaaac
     1621 ctgtttcagg tatcataata cccttcagac ggcatcgcgc cggctgaaaa tgacaacaac
     1681 cataccacca cgacaggaca cattcaatat gaaaacccaa atcagccttg ctgctgccgc
     1741 catcacactg ctcctttccg cctgcggagg cagcggaata ccttcccaac ccaaaggcga
     1801 aatttccgaa aaccgcaccg ccgcgttcaa atctatgatg cccgacttct cgcgtatggg
     1861 caaaatggtc aaaggcgaag aaccttacga tgtcgaaaaa ttcaaacagg cggcagcggc
     1921 gtttgccgac agcagcaaaa aaccgtttac gctttttgag tccgacccgc aaggcaacgg
     1981 gcgcgcactg cccgccgttt ggtcggatgg tgcaaaattt gaagccgaaa aaacaaaatt
     2041 cgccgccgcc gtcgaaaaac tcaacgccgc cgcccaaacc ggcaaactgg acgaaatcaa
     2101 agccgcctac ggcgaaaccg gcgcaagctg caaatcctgc cacgacagct tccgcgcgcc
     2161 cgaataacat ccaaaacaaa acaatgccgt ctgaagactt cagacggcat tttctttgcc
     2221 atttcagcac acgcaaagca atcagaaatt atcgtcaatc caaatgaaaa aacgctatcc
     2281 ttatttttac cccacgcact ccgccatttt ctattttgat ttgctatgcg attaattttt
     2341 atcgtcaaat gtcaaaatcg ggatgccgga ttgggtaaac agggcataca ccggcttgag
     2401 ccaataatct tctggaaggg aagccggttt caatgcgtaa acgtgcacat tgggaaaatc
     2461 cttcatcgtc aacgccgcgc cgctgaaaaa ggtataaatt tcatacctcg tatgcgggtt
     2521 tttcttaatt tcgcgcaaaa tatagtcttc gatgacatag ggcgaattta atgtggttac
     2581 gccggaaagc ccgtaggttt ggcgggggtg cggcgcgaca tattgtatgt tgaaatcttt
     2641 tgccgccttt tccgaaattt ccttcatctc tttgtcgggt gaacccaaaa gtatccgcac
     2701 cgtgccgccc gtttcgtccc ccgtcttcag ttcggacgca tcgaacagcg gcaggtaagt
     2761 catcttgcgg cggccgtcgt ccataatgtt tttcaaaccc ttgaatatcg tgtaatgctc
     2821 gtcggaagca ttgcgggttt tggcgatgct ccaatctccg atcatcatcc gggcaaaatt
     2881 ccgcttgatc gtcccgttta cagaaaactc atcgcccaaa tagctgctgc tttgaattaa
     2941 attgcctgtc ccgtcgtcaa aggttttgat ttccgcatcc gggtaagtgc tcaaaaaggc
     3001 ggcaatgctg actttttcca aacttgccaa ataaatccgc ttgactttcg gcagcagcat
     3061 cgactttacc ttcagctccg ccatcgtcgg aatgaaatta aacgatttgt tcaaaccgta
     3121 gggcaggtgg aaaaaatacg cccgctccgc cttatccttt atctgattga aataataatc
     3181 gtatttttca ttcctgtttt cagacatcag caccacataa aaccgctccc ccggatgctg
     3241 cgccataatc ctttccgcca ccttcatctg caatatggta tagcagaaaa tcagattgac
     3301 cggttccccc tcttcattga agagtttgtc cttcagcagg gaaaccgcat tccttttccc
     3361 ctgatttacc cggtcaaatg tataaaatat cccgaaacaa aaaacaatca aacacaacac
     3421 ggtcaaacaa gcctttttca agcccatatc cctaaaactc cattccgaca aattgaacat
     3481 acgccccgag tgtcccggga cggaaaaatg cggaatttta gcagatattc cgacagttac
     3541 cgaatattat agtggattaa atttaaatca ggacaaggcg acgaagccgc agacagtaca
     3601 agcagtacga caaggcgagg caacgctgta ctggtttaaa tttaaaccac tataaaatca
     3661 atttcaccga aaatatgccg ccgcacatca ggcacagcac ctccgaccgc cgccaaacgc
     3721 gggaaaacca tttccccctt cctttcacgc cgccgcgttc aaacacgcca agatcgaaca
     3781 aagcgcgaac aaagccgcca aaatatgcca taatagccac gcacattccc cgcgcaatgt
     3841 gttccaaaag aaaaaaccaa cctacaagag aataatccta tgactcaaaa atccaccatt
     3901 gtttataccc ataccgacga agcacccgcg ctggcgaccc aatcgctgct gccgattgtg
     3961 caggcttttg cccgccacgc cgatattgat gtcaaaacca gcgacatttc tctctccggc
     4021 cgtattttgg cggcgtttcc cgaatacctg accgaagcgc aacgcgtacc tgatgcgctt
     4081 gccgaattgg gcgaactggt gaaacaaccc gatgcaaacg taatcaaact gccgaacatc
     4141 agcgcgtccg taccgcaact gactgccgcg attaaagaat tgcaatctaa aggctttgcc
     4201 gttcccgact atcccgccga cccgcaaacc gatgaagaaa aagccgtacg cgaacgctac
     4261 gaccgcatca aaggcagcgc ggtaaaccct gtcctgcgtg aaggcaactc cgaccgtcgc
     4321 gcccctaaag cagtgaaaaa ctttgcgaaa aaaaatccgc acagcatggg cgcatggacc
     4381 aaagactcca aaacccacgt tgccaccatg caaagcggcg acttttttca taacgaacaa
     4441 tctgttatcg tacctgaagc gacttccgta tccatcgtat tcaccgacaa acaaggcaac
     4501 aaaaaagagc tgcgcgagcc cgtcgccctg aaagccggcg aaatcatcga cgcgaccgtg
     4561 atgagtaaaa aagccctgct cgccttcctt gccgaacaag taaaagatgc gaaagcaaaa
     4621 ggcgtgttgt tctcgctgca catgaaagcc actatgatga aagtgtccga cccgattatc
     4681 ttcggacacg ccgtcaaagt attcttcgcg cctgtttttg aaaaattcgg cgacaaactg
     4741 gctgccgcag gtgtcaacgt caacaacggc ttcggcaacc tgcttgccaa tctggacaaa
     4801 ctggatgcgg acacccgcac cgccgtcgaa gccgaaatcg ccgccgttta cgctgccaac
     4861 cccgatttgg cgatggttga ttccgacaaa ggcatcacca acctgcacgt tcccagcgat
     4921 gtcatcgtcg atgcctctat gcctgcgatg attcgcaatt ccggccgtat gtgggacaaa
     4981 aacggcaaag cgcaagacac caaagccgtg attcccgaca gcagctatgc cggcgtttac
     5041 caagcaacca tcgacttctg ccgcgaacac ggcgcatttg acccgacaac catgggtact
     5101 gtgcccaacg tcggactgat ggcgcaagcg gcggaagaat acggctcgca caacaaaacc
     5161 tttgaaatcg aagccgacgg tcaggttcaa gtcattgatg cggaaggaaa agtcctgatg
     5221 cagcacgacg ttgaagcagg cgacatctgg cgtatgtgcc aaaccaaaga cgctccggtc
     5281 aaagactggg tgcaactcgc cgtcaaccgc gcccgcctga gcaacacgcc tgccgtgttc
     5341 tggctcgatg aaaaccgtcc gcacgacaag agcctgctcg ccaaggttaa agcctacctt
     5401 gccgaactgg ataccaatgg cctcgacatc cgcgtcctcg ctcctgaaga agccgccaag
     5461 ttcagcttgg gtcgtctgaa aaacggcgaa gacaccatct ccgtaaccgg taatgtcttg
     5521 cgcgactacc tgaccgactt gttcccaatt ttggaactcg gcacaagcgc gaaaatgctg
     5581 tctatcgttc cattaatgaa cggcggcggt atgtttgaaa ccggcgcggg cggttctgca
     5641 ccgaaacacg ttcaacagtt cctcgaagaa aaccacctgc gttgggactc tttgggcgaa
     5701 ttcctcgcac tcgccgtatc gtttgaacat ttggcgcaaa aaaccggcaa tgccaaagcc
     5761 caagtcctcg ctgacacttt ggatgccgcc accgaaaaac tgctgttgaa cggcaaatcg
     5821 cctaaacgta aagccggcga actcgacaac cggggcagcc atttctacct taccctctac
     5881 tgggcgcaag aattggcgga gcaagacaaa gatgccgaac tgaaagccgc atttacgcca
     5941 ttggcagccg ctttgaccgc cgacgaagcg aaaatcgttg ccgagctttc cgccgtacaa
     6001 ggcaaagcgg tcgacatcgg cggctactac gcagccaatc ctgaaaaagc ggcacaagtg
     6061 atgcgtccga gcgtaacctt taatcaggca ctaaacgcct tataagcaca aaggtaaaaa
     6121 tgccgtctga acattcgtcg ttcagacggc attagttatc cctatctgcc tgattatgat
     6181 taaacgccgc acccattgga tat
//
LOCUS       DAWKST010000118         6158 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.118, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000118 DAWKST010000000
VERSION     DAWKST010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6158
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.118"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(294..728)
                     /locus_tag="WJL87_001554"
     CDS             complement(294..728)
                     /locus_tag="WJL87_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ7799966.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(731..1393)
                     /gene="exbB"
                     /locus_tag="WJL87_001555"
     CDS             complement(731..1393)
                     /gene="exbB"
                     /locus_tag="WJL87_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ7799967.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1459..2295)
                     /locus_tag="WJL87_001556"
     CDS             complement(1459..2295)
                     /locus_tag="WJL87_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ7799968.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPEPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPSNASAKADSEQGNGE
                     ETGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMENDE
                     QGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVKFE
                     LN"
     gene            2550..3188
                     /locus_tag="WJL87_001557"
     CDS             2550..3188
                     /locus_tag="WJL87_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ7799969.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3640..4440)
                     /locus_tag="WJL87_001558"
     CDS             complement(3640..4440)
                     /locus_tag="WJL87_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ7799970.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(5419..6060)
                     /locus_tag="WJL87_001559"
     CDS             complement(5419..6060)
                     /locus_tag="WJL87_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ7799971.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIEWPQKVMGYLMMMSEKSGVPLYFDRAL"
BASE COUNT         1646 a         1542 c         1265 g         1705 t
ORIGIN      
        1 gtactatttg tactgtctac ggcttcgttg ccttgtcctg atttttgtta atccactata
       61 aaaattagaa atgcacattt tcattattct cgcgcaggca ggactccaga cttacccatt
      121 tcagtaatgt ttgaaaataa aagaaaaatc agatgtttgt attcccgtct gcgcagaaat
      181 ggagacggcg ctctgtcgtc tcatttttgt tttaatcaac tatatatagc tgattaaaca
      241 taagaaatgc cgtctgaaag actttcagac ggcattcgtt caagcgtcga actttattgc
      301 gccttggttt cggttacaaa accgattttg gtgattcctg cctgacgggc ggcttctaaa
      361 gctttgttta cataatcgta ttccaccgcc ttgtctgccg caatcgccac aatcacgttt
      421 tcattctgct ccttggcggc tttcagacgg ctttccactt ccccgatttc cactttgctt
      481 gcagaatccc cgccgacata atagccgccg ttcgcatcaa tcgtcaggcg cagggggtct
      541 ttaggctgtt tgtcctgctt gtttgtctgc tcggacgcgg tcggcagttc caaagggatg
      601 gaatgcgtca gcaccggcat agtaatcata aacacaatca gcaacaccag catcacgtcc
      661 accaacggcg taacgttgat gtcggacatc ggagaatcgt cgccggaatt catcgaacca
      721 aatgccataa tcagctatcc ttttgattaa gcaggcggac gtgcaaatcg tgcgccatcg
      781 catccaaatc ctgggtcagt atttttgtgc cgcgattgag gaagttgtat gccaacaccg
      841 ccggaatcgc cacgaacaaa cccgccgccg tcgccaccag tgcctcgcca atcgggccgg
      901 caaccgccgc aatactcatc tgcccgcttt gcccgatatt gatcagggcg tggtaaatcc
      961 cccaaaccgt gccgaacagc ccgataaacg gcgcggtcgc gccgatggag gcaagcgcgg
     1021 tcatcccgta atcaaaccgg cgcataatct gcgccatact gttgcggatt tgaatgacca
     1081 aatactcgtt caacggcaaa gcctgcgcca gttcggacgc ttcgtttcgg cggtagttgc
     1141 ggtaagactg caatgcctct tgcgccagtt tggacaaagg cgcatcgacg gcgcgcactt
     1201 tttcgaccgc gtcgttcagc gacaaagtat cgcgcatatg ccgtttgacg gcggcattcc
     1261 ctttgcgcgc ccgatacagc ttgatgcagc gcaagacaac caaacaccac gttacgatac
     1321 tcatcaacag catcaacaca aacacaccga tcaggacggg atcgcccgat tcaaatacta
     1381 atttcaaatt cataatgatt ccaacactga aaaaaccaat caaacatcca agctgccgca
     1441 aaccgctgcg gcaaccgcct aattcaattc aaacttgacg gggactttaa actccgtcca
     1501 ggcattggct tgaaaatgcc cgttttgcgc cgccttgcgt gccgcattgt ccaaccgaga
     1561 aaaaccactg cttttcacga ttttaacgga ctcgacatga ccgcccggag aaaccaaaac
     1621 gctcaaaaca accgtaccct gctcgtcatt ctccatagaa agcgtgggat aagccgggcg
     1681 cggaatgctg ccgttggcgc gtaaaggatt gcctttgctg ctgccggctc cttccccgtg
     1741 ttcgcctttg acaccgccgc tacctttacc gctgccttct ccgcgccccg ttccgtctcc
     1801 tttggtaccg gtttcttccc cattaccctg ctcgctgtct gctttggcag aagcattgct
     1861 gggatgttcg gcaggttttt cagacggctt ctcgaccggt ttttccgccg gtttcgggac
     1921 aggcttcgct tccggcttag gctctggttt cggtttttct tcgggtttcg gcttttcttc
     1981 aggtttcggc tcttccttag gctgctgaat atccgcatcc gcctttttcg taaccaccgg
     2041 cttcaaaacc ggcttgggcg gctcgacagg tttgggcggc tcaggctcag gttgcggctc
     2101 aggcgcagca ggcgcacctg cgccttcggg tgcaccgtcc cctccgccaa aatcacccaa
     2161 atcgacaaat tcgataacat ttcctgactc tatcacgggc agcttgtgcg cctgccagag
     2221 caatgccacc attgccaaat gcagcagtgc gacggaaaac acgactgcgg gggttaaaat
     2281 tcgttcttta tccataattc gggcataata atagcaacaa ttcctatttg caacctattt
     2341 ttacaatttt tggtcatatg aatgtctgtt ccgttcacag gcaaaccgtg tttaaacgct
     2401 gtattacagc aaatcatcag ataacgggcc ggcagaaaaa atgattccgt ctgatttctt
     2461 attccaataa aatcaggtta gatgatatat tgccgcttct gtctgtcagc cgtttcgggc
     2521 tgcacaccac atctgttcaa aggaaaacca tgtttcaaaa ttttgatttg ggcgtgtttc
     2581 tgcttgccgt cctgcccgtg ctgctcgcca ttaccgtcag ggaggtggcg cgcggctata
     2641 cggcgcgcta ctggggagac aacactgccg aacaatacgg caggctgaca ctgaaccccc
     2701 tgccccatat cgatttggtc ggcacaatca tcgtaccgct gcttactttg atgttcacgc
     2761 ccttcctgtt cggctgggcg cgtccgattc ctatcgattc gcgcaacttc cgcaacccgc
     2821 gccttgcctg gcgttgcgtt gccgcgtccg gcccgctgtc gaatctggcg atggctgttc
     2881 tgtggggcgt ggttttggtg ctgactccgt atgtcggcgg ggcgtatcag atgccgttgg
     2941 ctcaaatggc aaactacggt attctgatca atgcgattct gttcgcgctc aacatcatcc
     3001 ccatcctgcc ttgggacggc ggcattttca tcgacacctt cctgtcggcg aaatattcgc
     3061 aagcgttccg caaaatcgaa ccttatggga cgtggattat cctgctgctt atgctgaccg
     3121 gggttttggg tgcgtttatt gcaccgattg tgcggctggt gattgcgttt gtgcagatgt
     3181 tcgtctgact ggctttcaga cggcataaac gctccagaaa acgcggcagg acatattgcc
     3241 ctgccgcgtt ttcctgtagt gtaatcttat ttttttcatc attattagaa ccaagttgca
     3301 tgataatacc tttcattaac tgaaacactg attaagaaac tccagtctgt ctaatgatga
     3361 ggttttcaca tcgccaaaac ttgccaatca aatgctggat ttattgccgt ctgagatttg
     3421 gtcaaatcca aaggcgacat tcttagaccc tgtgtgtaaa tcaggggtat ttttgcgtga
     3481 aatcgtcaaa cgcttggatg aaggcttgac caatcaaata ccagataaac aaactcgcat
     3541 taaccacatt ttaaaaaatc aagtttttgg aagtactgcc acgtatgtag gtagctttga
     3601 ccgatatttg cataaaaact cctttgctgg tgaaaggaat tattttgcca attttaaaat
     3661 atttctagca ccaaatagta caatgacaaa gacaatcatg ccaatgatta aatcaggata
     3721 gctagaatga gtcaataacg tcaatgctcc cgccgctatc acaccgatat tgatgataat
     3781 gtcattggat gtaaaaatca tgctggcttt gatatggatt tctttatttt gatttttgct
     3841 cagtagatat aagcacagcc agtttgcaat caatgccaaa aatgccgtgc caatcatcag
     3901 ttgataattg ggcagctgct cagcaccgat aaaacgccta atcacttcta tcaccccaaa
     3961 taacgccaat attatctgcg ttatccccgc caaaaatgcc acacgttttt tatacgccag
     4021 cgtcatacca atggctgata gcgccaatat atagacaaag ctgtccgcca gcatatctag
     4081 actatcagca atcagcccca tagaattagc aaaaatacca accgaacact ctatgataaa
     4141 aaacacaaag ttaatcatga gcacttgata taataatctt ttttctaagt gctcatcagg
     4201 cttgttaaac actatcttat caacaatcac ttcggtggaa atgatatgac tatcaaaatt
     4261 aagcggttca agtacttgta aaatcgttgt atcttgatta tcgtgataga cggttaagca
     4321 ccgcccagca atatcaaact gtaattcata aatatcagac acatctttta aacgcatgcg
     4381 aatgagctgt tcttcggacg ggcagtccat tttggtaatg ttaaaaatgg tctttttcat
     4441 ctatttagtt ccttgttttg atcaggttgg ctcaaataaa tctgtgttta tattgctgct
     4501 tggtaatttt tggatggttt gagtaaattg attaggttaa aatttacctt tggaagtacc
     4561 gccacgcata atagtttaga tatgtttata atctctggat aaaaaaacgt aataagtgtt
     4621 tgctggataa caaagtccaa accaatagca gataaaataa ggcatccacc ccctttcttc
     4681 attaaggata agagatatcg agaaacaaat cataactaaa cagaaaaaat tttggagata
     4741 aagccatttc attcccctat tcaagaatct ggccaagata ggtattttgt atactctaga
     4801 aaagaaaggc atttccaagg gaaacatatc agataaaaac ttttgtttat tttttactat
     4861 agatagaacc ctatttctca agataaatcc aaatgcaatg ccaattatca ttattaataa
     4921 acaaaggata gtataactat ggataacctt cattaaacaa aaagaagaaa tggcagataa
     4981 attacatacg atatagtgga atataaaata tttacggtct aaaccttgtt cagttgcaat
     5041 ttttttatac ttaccttgcg caaaaaaatc aaagaaatcc attaaactat ctttcacatt
     5101 agaaatttaa aagctaaata atacaacaaa caatgtgaag tactattcat ggtttattta
     5161 aaaaataata ctattctgaa cattatttag atacagaaat taacaaatta gaactaaaca
     5221 agcttttaaa tactttaatt ttattggaaa gctataaaag gaactataac tttacacact
     5281 agtcacttct ttttaagagg caaaagggat tgggaaggtc gtcttggaga taagcactgg
     5341 tatttcggcc aatggtaaat agagtttacc tcaaataggg tagaacctcc ttcatctgtc
     5401 agttaataac agccactttt acaatgccct gtcaaaataa agcggcacgc ccgatttttc
     5461 actcatcatc atcaaataac ccatcacctt ttgaggccat tcgataccgc gcaccacggt
     5521 cagattcctc aaaacgggga aaaccaaaat atcctccatg ccgattccgc cgttgatgcc
     5581 gtctgaagca ccgtccatca aattctccaa ctcttgcaaa tctgcgttta tgcgttcgag
     5641 atattgggcg gttttattca aattggcgga aaagctgccg atgcttttct cttttttgtc
     5701 tgtaaaatat tttaccgctt ccggcgttgc aaattcgggc agcccgattt tgatcacgcg
     5761 cggctgcacc agtttgtcgt tgtatccgcc caccttgtcc agccacgccc gtatctcggg
     5821 gcggacttcg tctttcagac ggtcttcgcg gtcgaaatgc cgcacaatgt ccaaactctc
     5881 gcccataaac gaaccgtctt ctttttgcag gacgggcact tgtttcgcac cgatcatacc
     5941 gatcggcgtt gcctcgtcgt cgtttgccag cacggcttct tcaacgtccg cgccaaacag
     6001 cccggcagcc atccgcgcac gcacacaaaa cgggcaatgg tcgtaaatat acagtttcat
     6061 caaaatattc ctcgtcaacc tgtcggtacc ggctacctta acaccccgcg ccacccgaaa
     6121 caagtttatc ttcccgccta tgcaccgtaa ataaataa
//
LOCUS       DAWKST010000119         6118 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.119, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000119 DAWKST010000000
VERSION     DAWKST010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.119"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(152..2080)
                     /gene="dnaK"
                     /locus_tag="WJL87_001560"
     CDS             complement(152..2080)
                     /gene="dnaK"
                     /locus_tag="WJL87_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ7799972.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDHIIAEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTSD
                     IDDVILVGGQSRMPKVQEAVRDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKTEALGAASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            complement(2288..2560)
                     /locus_tag="WJL87_001561"
     CDS             complement(2288..2560)
                     /locus_tag="WJL87_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799973.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2746..3432
                     /locus_tag="WJL87_001562"
     CDS             2746..3432
                     /locus_tag="WJL87_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7799974.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRNTKNLSVISVKGDSMEGVLNDGDSILVN
                     HSENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNHPADDVEII
                     GRVEWFGRTI"
     gene            3564..3902
                     /gene="erpA"
                     /locus_tag="WJL87_001563"
     CDS             3564..3902
                     /gene="erpA"
                     /locus_tag="WJL87_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ7799975.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            3919..4065
                     /locus_tag="WJL87_001564"
     CDS             3919..4065
                     /locus_tag="WJL87_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799976.1"
                     /translation="MPSERSDGIFTFGKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            4062..4526
                     /locus_tag="WJL87_001565"
     CDS             4062..4526
                     /locus_tag="WJL87_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ7799977.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPKVDCGLIRECLRENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWEKRAELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     GDGEIPEYLELHSEREDDISAPSQAEPPPRNIKPLVFGLLIAFLGYLLGKIF"
     gene            4561..6072
                     /gene="ubiB"
                     /locus_tag="WJL87_001566"
     CDS             4561..6072
                     /gene="ubiB"
                     /locus_tag="WJL87_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ7799978.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPVASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAVWVERLFADGKRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
BASE COUNT         1378 a         1359 c         1644 g         1737 t
ORIGIN      
        1 aaaaatttcc agagaaccga tacaacagtt ggaacttggg tttgggaata ttacggtaga
       61 tgaacttgga acctctgtta tgctatggtc ttttatctca attgaaaaaa gcgcgaatca
      121 aacggttcgc gcttttttca gacggtatta attatttttt gtcgtctttt acttcttcaa
      181 agtcggcatc tacgacatca tcgtctttct ttgcagaagc attggcttgt tcgctttcgc
      241 ctgcttgggc ttcagcttgt gcttgagcgt aaaccatttc ccccagtttt tggctggctg
      301 cgcccagcgc ctcggttttg gcatcgatag cggctttgtc gtcgccttta actgcttctt
      361 cggcttcttt cagcgcggct tcgatttttt ctttctcggc tgcgtcgagt ttgtcgccgt
      421 agtcggccaa agattttttc acagagtgaa tcagggcttc agcttggttg cgggaagcga
      481 ccaattcagt cagtttttta tcttcctcgg cattggcttc ggcatctttc accatgcgtt
      541 cgatttcttc ttcgctcaaa cctgaagaac cttggatggt gatgttggct gctttgccag
      601 tgcctttgtc tttggcggaa acgtgcagga tgccgttggc gtcgatgtcg aaggtgactt
      661 cgatttgcgg cataccgcgc ggtgcgggtg cgatgtcgcc caagttgaac tgacccaaag
      721 atttgttggc agaagcgcgt tcgcgttcgc cttgcagtac gtggatggtt actgcgcttt
      781 ggttgtcttc ggcggtagag aacacttgcg acgctttagt cgggatagtt gtgttctttt
      841 gaatcagctt ggtcatcacg ccgcccatgg tttcgatacc caaggacaga ggggttacgt
      901 ccagcagcaa tacgtcgctg cggccgccgc tcaatacttc gccttggatc gctgcgccta
      961 cggcaacggc ttcgtcaggg ttcacgtctt tgcgcggttc tttgccgaag aaatctctaa
     1021 cggcttcttg tactttcggc atacgggact gaccgccgac caaaattacg tcgtcgatgt
     1081 cgctggtgct caagccggca tctttcaatg cggtgcggca aggctcgata gagcgggcaa
     1141 tcaggtcttc aaccaggctt tcgaatttgg cgcgggtaat tttcatcgcc aagtgtttcg
     1201 ggccggttgc gtccatggtg atgtacggca ggttaatttc ggtttgctgg ccgctggaca
     1261 attcgatttt ggctttttcg gcagcttctt tcaggcgttg tagagccatc acgtcttgtt
     1321 tcaaatcaat gccttgttct tttttgaact cggcgatgat gtggtcgatg aggcgttggt
     1381 cgaagtcttc accgcccaag aaggtatcgc cgttggttgc caatacttcg aattgtttgt
     1441 cgccgtcgag gttggcgatt tcgatgatgg aaatatcgaa agtaccgccg cccaagtcat
     1501 atacggctac tttgcggtct ttgttgtcgc ctttgtccat accgaatgcc aaagcggctg
     1561 cggtcggctc gttgatgatg cgtttcacgt ccaaaccggc gatacggcct gcgtctttgg
     1621 tggcttgacg ttggctgtcg ttgaagtagg cagggacggt aatcacggct tcggttactt
     1681 tttcgcccaa gtaagcttcg gcggcttctt tcattttacg caggacttct gcggaaattt
     1741 gaggaggaga cagctctttg ccttgtgctt ttacccatgc gtcgccgttg ttggctttga
     1801 tgatttcgaa aggcatagat tcgatgtcgc gttggacttc tttgtcttca aatttgtggc
     1861 cgatcaaacg tttggcggcg taaatagtgt ttttggcgtt ggttaccgct tggcgtttgg
     1921 caggcgcacc gacgaggatt tcgccgccgt ccaaataagc gataacggac ggcgtggtgc
     1981 gtgcgccttc tgcgttttcg atcactttgg tttgaccgtt ttcggaaatg gccaaacaag
     2041 agttggttgt acctaagtcg ataccgatta cttttgccat gtggataatc ctatttgatt
     2101 ttgcttattt tgagaaatat gttggaacat tttgtcccga tgggctgtaa atagggcggg
     2161 cggcgggctg tttcaagcta cagcatggct ataagtatat aactttatga atatattggt
     2221 tttatatttg atttaataca tttggctcca atgcattcaa gcataatgtt tcaaatggca
     2281 ggcaggttta ttcatagacg atgccggcga gcatttcctg ttcgttcaag ttgccgtact
     2341 ctttttccca gtcgtgtgaa gactcgatga tgtcgcattc tttggaaagg gagacttgtt
     2401 ctgcatccat atctttggcg ttcagtatgt tgaattgttc gcatagggat gcggataaag
     2461 tgatgtcagg ctgtttggct tcagaacggt tttcttggaa ggcaaagcag aatgcggtaa
     2521 atgccgcagc atagataaga tatttgccgg ttttcttcat tttctatcct ttttctgtca
     2581 actcaggatt aaacctatgg aaaatcttaa aaattatgta ttgagtaaga aaaatcataa
     2641 tttaaattta gtttatcata gttgttccgt ttttgggata gctaaggtaa aatatatttc
     2701 atgtttactt tagatgattg aatgaagggg agcggaagga tatttatgga aacctttaaa
     2761 gacagactgg tttttttatg gaaaagcgaa gcgaagcagg caaaaatcgc atccgatatt
     2821 gaaatgacga ttgcgggctt cagcaggata tggaatgaag gcggtctgcc gaagtctgaa
     2881 acattaaaaa aaatcaagca gttgaagggg tgtagtatcg attggctgct gaccggggag
     2941 ggtaatccgt ttccggatga agccccaaaa aaatcccttg cttacgatac cttgggaaat
     3001 gaagtcgata cggacgagtt tgtctttgtg ccgagatatg atattcgggc ggctgcggga
     3061 tacgggcagt ttgtcgatca tgaggaaccg gtatttacaa tggcgttcag acggcattgg
     3121 attgagaatt atgttacccg caatacgaaa aacctgtctg taatttccgt caagggggat
     3181 tcgatggagg gggttttgaa tgacggcgat tcgattttgg tcaaccatag cgagaatacg
     3241 ccgagggacg gactgtatgt gttgcggatt aatgaaaatc tgctggttaa acgtttacag
     3301 attgtaccgg gcgggataat caacgttatt tccgccaacg aggcttaccc tgcttttgaa
     3361 attaatttga atcatcctgc cgatgatgtg gaaatcatcg ggcgggtcga gtggttcggc
     3421 aggacgattt gagtttgggg cttgaaatcg ggagcggtca aacttatctg taaggcagtt
     3481 ccttttcagg gaacaagccc ccgcttgcct ttgaaggggg tttgagagag aatgcagaaa
     3541 atattatatt aaggaataac accatgtcgg atgaaagccc tattattttt actgacagct
     3601 gctgtgccaa agttgccgat ttgattgccg aagaaaacaa tcccgatttg aaattgcggg
     3661 tttttgtcaa tggcggcggc tgttcgggtt tccagtacgg atttactttt gatgaaatca
     3721 aaaacgacga cgattttgaa attgagaaaa acggtttggt ctttttggtc gatccgatga
     3781 gctatcaata tctggtcggc gcggaaatcg actatacgga aagtttgcag ggttcgcaat
     3841 tcgtcatccg caatccgaat gcggaaacaa cctgcggttg cggatcgtcg ttttccgtat
     3901 gaccgcttgg tttgtgtgat gccgtctgaa cgttcagacg gcatttttac ttttggaaaa
     3961 tatattatcg ggatgaattc acatataatc cgattgtttg aagatgaatc gggtttcccg
     4021 aaaggaacgg gcggaacggt atcaggcgta tttgttccct tatgattgag atgagtaaag
     4081 attaccgaaa cgatttgtac gatgtatatg tttcctaccc gcccaaagtg gattgcgggc
     4141 ttatccggga gtgtctcagg gagaatctcg gcgaagaaaa ggcggaagga ttgattgagt
     4201 ccctcgattc caaaccgcaa gtgctggttg aggaaaaatg tacctgggaa aagcgggcag
     4261 agttgcacga ttatttcagc tatttgggtt tggatattat tactcggaga tatatggagt
     4321 tagaaacggt cgtgccgccg gaggaagggg agggcgaagg agaaggcggg gatggggaaa
     4381 tacccgaata tcttgaactt catagcgaac gggaagatga tatttccgca ccttcgcaag
     4441 ccgaaccgcc gccccgcaat atcaaaccgc tggttttcgg gctgctgata gcctttttgg
     4501 gctatctgct cggtaagatt ttttgattgt ccgataaatg ctgtattcgg gattttatat
     4561 atgaaatggt tgaaacgcct gacggttatt gtcgggactt tttaccgcta tcggctggca
     4621 ggtctgtgcg cttcgctgat gggtagcggt tggatatgcg ctctgctgaa aatgatgccg
     4681 cagtcgtcca aattgaaaaa cgaaccgcct gccgtccgtc tgcgccttgc cttggaaagc
     4741 ctggggccga ttttcatcaa gttcgggcag gttttgtcca cacgccctga tttgattccg
     4801 cacgattacg ccgtcgaact ggcaaagctg caagacaaag tgccgccttt tgacgcgcgg
     4861 ctttcgcgtg aacaaatcga aaaatcgttg ggtcagtcca tcgaaaagct gtatgcggaa
     4921 tttgaaaccg agcccgtcgc cagcgcgtcc atcgcccaag tacacaaagc ccgcctgcat
     4981 tcgggcgaac gagtggctgt caaagttttg cgccccaacc ttttgcccct tatcgaacag
     5041 gatttgtcgc tgatgcgttt cggcgcggtt tgggtggagc gtctgtttgc cgacggcaag
     5101 cgtctgaagc cgctcgaagt ggtggcggag ttcgacaaat acctgcacga cgagttggat
     5161 ttgatgcgcg aagccgccaa tgccggacag ctcgggcgca atttccaaaa cagcaatatg
     5221 ctgattgtgc ccaaggtgtt ttacgactac tgcaccagcg acgtgctgac aatcgaatgg
     5281 atggacggca cgccggttgc cgacatcgcc aaactcaaag cagacggcat cgatttgcac
     5341 aaactcgccg attacggcgt ggaaatcttt ttcacgcagg tcttccgcga cggctttttc
     5401 cacgcggata tgcaccccgg caatattttg gttgccgccg acaaccgcta catcgccctc
     5461 gatttcggca tcgtcggcac gctgaccgat tacgacaaac gttatctcgc catcaatttc
     5521 ctcgcctttt tcaaccgcga ttaccggcgc gtcgccaccg cccacatcga atcgggctgg
     5581 gtgcctgccg acacgcgtgc ggaagaattg gaagccgccg tccgcgccgt gtgcgaaccg
     5641 gtgttcaaca aaccgatttc gcagatttcc ttcggcttgg tgctgatgcg cctgtttgaa
     5701 gtcagccgcc gcttcaatgt cgaaatccaa ccgcagcttg tgctgctgca aaaaacgctg
     5761 ctcaacatcg aaggcttggg ccgccagctc gatcccgatt tggacttgtg gaaaaccgcc
     5821 aaaccgtttt tggtgaaatg gatgaacggg caggtcggcc ctaaagccct ttggcgcaac
     5881 ctcaaaaacg aagcccccga ctgggcgcaa atcatccctt cattgccgcg caaaatcagt
     5941 gcgttgattg atgaaaaccg ccagcaggaa atgcgtgatg cctatatcca tttggtcaaa
     6001 gtgcagcagc ggcaaagcct gtggctggct gtgattgcgg ttgttttgct gctgattttg
     6061 cttttgaaat aggctttgtc cgaatcatcg cccgactccg cccgtttata aggaaatc
//
LOCUS       DAWKST010000120         6106 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.120, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000120 DAWKST010000000
VERSION     DAWKST010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.120"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(108..809)
                     /locus_tag="WJL87_001567"
     CDS             complement(108..809)
                     /locus_tag="WJL87_001567"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ7799979.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGKFAAYEGELAGK
                     RMVLALSGIGKVNAAVATAWIIREFAPDCVINTGSAGGLGKGLKVGDVVIGTETAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDDLLVGKAKRAARTFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            995..2107
                     /locus_tag="WJL87_001568"
     CDS             995..2107
                     /locus_tag="WJL87_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ7799980.1"
                     /translation="MTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRA
                     ITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDI
                     SLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            2141..3118
                     /locus_tag="WJL87_001569"
     CDS             2141..3118
                     /locus_tag="WJL87_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ7799981.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFVAKA
                     LLCETPAQGCKPCGECMSCHLFGQGSHPDFYEITPLADEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHTADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLDVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TVSGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            3122..3472
                     /locus_tag="WJL87_001570"
     CDS             3122..3472
                     /locus_tag="WJL87_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEZ7799982.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTDDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGSTISGSRPTFTM"
     gene            3477..4256
                     /locus_tag="WJL87_001571"
     CDS             3477..4256
                     /locus_tag="WJL87_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ7799983.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     AEVSAIAEVYEHIYCTIGVHPDSKEAEEFSIAEMVAAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRAV"
     gene            4282..4496
                     /locus_tag="WJL87_001572"
                     /pseudo
     CDS             4282..4496
                     /locus_tag="WJL87_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
     gene            4597..4908
                     /gene="grxD"
                     /locus_tag="WJL87_001573"
     CDS             4597..4908
                     /gene="grxD"
                     /locus_tag="WJL87_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ7799984.1"
                     /translation="MASIHDQIKEIVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            5014..5640
                     /gene="upp"
                     /locus_tag="WJL87_001574"
     CDS             5014..5640
                     /gene="upp"
                     /locus_tag="WJL87_001574"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ7799985.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            5663..5983
                     /locus_tag="WJL87_001575"
     CDS             5663..5983
                     /locus_tag="WJL87_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ7799986.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
BASE COUNT         1516 a         1574 c         1605 g         1411 t
ORIGIN      
        1 aaatgccgtc tgaacaaaca gcggtgcgtc cgaatatcgg gcgcaacgtg gaaatagccc
       61 gatattctaa cggaaaaccg ctgttttggc atgatttgat aaaggtttta taaagatttg
      121 acgatttctg ccaccatttt tgcagaactt gccgccgccg ttttcaaaaa ttcgtcaaag
      181 ctgatgtctg ccttttcgtc cgccgaatcg gaaaccgcgc ggatgatgac gaaaggcgtt
      241 tccaactgat gacaggtttg ggcgattgcc gccgcttcca tttctaccgc cttaacttcg
      301 gggaagtgct tgcggatttc cgccacgcct tcgctgctgt ggacgaagcg gtcgccgctg
      361 acaatcagac cttgttctac cgccgcgcct tcaaacgtcc gcgccgcccg ttttgccttg
      421 ccgaccaaga ggtcgtctga aacgaatacg gcgggcagtt gcggcacttg tccccaaaca
      481 tagccgaatg cggttacatc gacatcgtgg tgtgcggttt ccgtgccaat caccacgtcg
      541 ccgactttca aacccttgcc caagccgccc gcgctgccgg tgttgataac gcagtccggt
      601 gcgaattcac ggataatcca agccgttgca accgccgcgt tgaccttgcc gatgccgctc
      661 aatgcaagca ccatgcgttt tcccgctaat tcgccttcat aggcggcaaa cttgccgaaa
      721 gagacggctt tgacattttc catcatctcg cgcaaaagct cgatttcttg ttccattgcg
      781 ccgataacgg ctactgtttt caaagacata ttgctgacct gttgtgaatt tcggatagaa
      841 tgcctgatta tacacgctaa cacggcagga ttgagtggag gtggtttgtc cgtgccgtct
      901 gaaacggttt cagacggcac ggcgggtttt tggtagaatg ggaaggtaca gattgtttga
      961 agattagggg acgaggatgt ttaccgatga aaatatgacc gcaaaggaag aactgttcgc
     1021 atggctgcgc catatgaaca aaaacaaagg ttccgacctg tttgtgacga cccatttccc
     1081 gcccgcgatg aagttggacg gcaaaatcac ccgcatcacg gacgaaccgc tgacggcgga
     1141 aaaatgtatg gaaatcgcct tttcgattat gagtgcgaag caggcggaag aattttcatc
     1201 gaccaacgag tgcaacttcg ccatcagcct gccggacacc agccgcttcc gcgtcaatgc
     1261 gatgatacag cgcggcgcga cggcgttggt attccgcgcg attaccagca agattcccaa
     1321 gtttgaaagc ctgaacctgc cgcctgtctt gaaggatgtc gcgctgaaaa aacgcgggct
     1381 ggttattttt gtcggcggca ccggctcggg caaatcgact tcgcttgcct cgcttatcga
     1441 ctaccgcaat gaaaattcgt tcggacacat catcaccatc gaagacccga tcgagtttgt
     1501 ccacgaacac aaaaactgca tcatcaccca gcgcgaggtc ggcgtggata cggaaaactg
     1561 gatggcggcg ttgaaaaaca cgctgcgtca ggcgccggat gtgattctga tcggcgaaat
     1621 ccgcgaccgc gaaacaatgg actacgccat tgcctttgcc gaaacggggc atttgtgtat
     1681 ggcgacgctg cacgccaaca gcaccaatca ggcactcgac cgcatcatca actttttccc
     1741 cgaggagcgg cgcgaacaat tgctgacgga tttgtcgctc aaccttcagg cgtttatttc
     1801 gcaacgcctc gttccgcgag acggcggcaa gggcagggtg gcggcagtcg aggtgctgct
     1861 caattcgccc ctgatttcgg agttgattca caacggcaac atccatgaaa tcaaagaagt
     1921 gatgaaaaaa tccactaccc tgggtatgca gaccttcgac caacaccttt accaattgta
     1981 tgaaaaaggc gatatttccc tgcaagaagc attgaaaaat gccgattccg cacacgattt
     2041 gcgtttggcg gtacagttgc gcagccgccg cgcgcaaagt tccagccccg atttggaact
     2101 gctctgatgg cggtatggat ttccggacgg atggtttgaa atgatttatc cgtggcataa
     2161 tgagcaatgg cggcagattg cggaacattg ggagcgtcgt cccaatgcat ggctgtttgc
     2221 cggcaaaaaa gatacgggga aaactacatt tgcccgcttt gtggcgaagg cactgttgtg
     2281 cgaaacccct gcacagggct gcaaaccctg tggcgaatgt atgtcctgcc atctgtttgg
     2341 acagggaagc catcccgatt tttacgaaat cacgcctctg gcggacgagc ccgaaaacgg
     2401 acgcaaactg ttgcagatca aaatcgatgc cgtcagggaa atcatcgata atgtgtacct
     2461 gacttcggta cggggcggtt tgcgcgtgat cctgattcat cctgcggaaa gtatgaatgt
     2521 ccaagccgcc aacagtttgt tgaaagtgtt ggaagagccg ccgccacaag tggtcttttt
     2581 gctggtcagc cacacggcgg acaaggtttt accgaccatt aaaagccgct gccggaagat
     2641 ggttttgccc gcgccttccc atgaagaggc attggcatat ctgcgtgaaa ggggtgtggc
     2701 ggaacctgag gaacgtctgg ctttccattc cggagcaccg ctgtttgatg aggcggacgg
     2761 tgtccgtgcg ttgcggatta aactgttgga tattttggca gaaccaaggt tgttgaagat
     2821 tttggattac gccgcgcttt tcgataagga gaaacttccg ctcgacgtat ttgtcgggtg
     2881 gatgcagaag tggctggtcg atttgggatt gtgcctgcaa cacatgaaac ccgtctatta
     2941 tcccgcttat gaagacaggc tgcttcagac ggtatccggt ttccgcccgc gcaatgtatt
     3001 tgcggcggag gatatgctca aacagcttgc cccctacggg tttcatactt taaatgttaa
     3061 aatgcagatc gagcatctgc tcatcaacta tttggaattg aagaaagaga acgggtgaat
     3121 tatgtcagac ggacaaaata ttccggcaaa aatgatgtcg ttgcagctga aagacatgaa
     3181 tctgctgtac agctcctata tgccgttttt ggagcatggc ggtctgtttg tgcagaccga
     3241 cgacgtattt tccatcgggg acgatattct gcttgccgta gaaatcctca acttccccaa
     3301 actgttcctg ccgaccaagg tcgcctggat taatcctgcg cgtacttcct ccaaacccaa
     3361 aggggtgggg ctggcattca caaaacacga aaactgcctg aaagtcaaag accagatcga
     3421 agtcgaactg ggcagcacaa tcagcggcag cagacctacg tttaccatgt aacgccatgc
     3481 atatcatcga ttcgcactgc cacctcaatt ttgaaggttt gaaagaacgc ctgcccgaag
     3541 ttttgtccaa catggaagca aacggcgtgg ggcaggcgct cgccatcagc gtcagcaggg
     3601 aaagttttgc cgaagtatcc gctatcgccg aagtttatga acatatttac tgcacaatag
     3661 gcgtacatcc cgacagcaag gaagccgaag aattttctat tgcggaaatg gttgctgcag
     3721 ccgcccatcc gaaagtggtc ggcattggcg agacgggctt ggattattac tggtgcaaag
     3781 gcgatttgtc ctggcaacac aaacgctttg cagaccacat cgaagcagcc aatcaaaccg
     3841 gactgcccgt tatcgtccat acgcgtgatg cggcggcgga caccttgtct atcctgaaag
     3901 aatgccgggt taattcgggc gttatccact gtttttccga agacatcggt tttgcccgtg
     3961 cagcaatgga tttggggctt tatatttctt tctcgggaat cgttaccttt aaaaacgcac
     4021 ccttggttca ggaggcggca aaatacgttc cggacgaccg cattttggtg gaaaccgatg
     4081 cgccgttcct cgcccccgtt cccaaacgcg gcaggcagaa cgaaccggct tttgtgcgcc
     4141 ataccgccga acatatcgcc aaattgcgga accaaacatt ggaacaggtt gcggcatata
     4201 cgacggaaaa cttttaccgg ctgtttaaaa aagtacccga tatgcgggcc gtctgaccct
     4261 gtaccgacga taaggaaaac catgaaggca attcatccgt atgcatgtcc gcgctgctgc
     4321 cggctgcctg ccaacacgtt tcggacaggc atggcaaatt ccgcttccaa attctgcatt
     4381 gccaaaggcg gcagacggga agcaaaaaag acgaaagcgg cggcggatat gccctgtgcc
     4441 atttgccgga cagcaggatt gtcgaggagt gggaatattt ccgttcacaa cattgatact
     4501 gcgcgatata cggcaaatat tgtgggaagt ttccgctttt gcgtataatg cgccctacct
     4561 gacaaatttt gtcaacttta tcaaaaggaa taagcgatgg cttccatcca cgaccaaatt
     4621 aaagaaatag taacgacaca ccgcgtcgta ttgtttatga aaggtacgaa gcagtttccg
     4681 caatgcggtt tctcttcccg cgccgtgcaa atcctgaacg cggcgggctg caccgattac
     4741 gttaccgtca acgtattgga aaatcccgaa gtacgccaag gcattaagga atacagcgat
     4801 tggccgacca tcccccaact ttatgtgaac ggcgagtttg tcggcggttc ggacatcctg
     4861 atggaaatgt atgaggcagg cgagctgcaa gagctgctga aagcctgatg gattcggcaa
     4921 tgccgtctga acgtgtttca gacggcattt tcttttccgg caaatcaaaa aaaagtataa
     4981 tggcgcgtct caaaatcaca ctggaacacc gcgatgaacg ttaatgttat caaccatccg
     5041 ctcgtccgcc acaaattaac cctgatgagg gaggcggatt gcagcaccta caaattccgg
     5101 acgcttgcca tcgagctggc gcgcctgatg gcatacgagg caagccgtga ttttgaaatc
     5161 gaaaaatacc ttatcgacgg atggtgcggt cagattgaag gcgatcgcat caagggcaaa
     5221 acattgaccg tcgttcccat cctgcgtgca ggtttgggta tgcttgacgg cgtactcgac
     5281 ctgattccga ctgccaaaat cagcgtagtc ggactgcagc gcgacgagga aacgctgaaa
     5341 cccgtttcct acttcgagaa attcgtggac agcatggacg aacgtcctgc cctgattatc
     5401 gacccgatgc tggcgacggg cggttcgatg gttgccacca tcgacctttt gaaagccaag
     5461 ggctgcaaaa acatcaaggc actggtgctg gttgccgcgc ccgagggtgt gaaggcggtc
     5521 aacgacgcgc atcccgacgt tacgatttac accgccgcgc tcgacagcca cttgaacgag
     5581 aacggctaca tcatccccgg cttgggcgat gcgggcgaca agattttcgg cacgcgctaa
     5641 ctgactgatt ttcggagttg atatgaattt tcaagactat ctcgccacat ttccttcaat
     5701 cgaccatctg ggcggtttgg acgttcagga tgccgacggc aaaacggttc accacattcc
     5761 cgccgttcag ggtaagctcg gttcgctcaa gctgtacaat gctttggcag aacgttttga
     5821 cggaaaattg gataaagaag cggcagaaca gggtttgata tggtttgccg aacacgttgc
     5881 cgacgcgcgc gcccatcccg gcaagcatcc caatatcgac ctgcttgaaa aagttgtgca
     5941 aagcggcgaa accttgctgc tcaagccgct tgccgcgcaa taattttcga ccatgccgtc
     6001 tgaaatccgt ttcagacggc attttatcgg aaagaagacc gtaaaacggg cattttcttt
     6061 tctatttcag gatacgggca atgatgtttc aacacacagg acgata
//
LOCUS       DAWKST010000121         6076 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.121, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000121 DAWKST010000000
VERSION     DAWKST010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.121"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            66..260
                     /locus_tag="WJL87_001576"
     CDS             66..260
                     /locus_tag="WJL87_001576"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799987.1"
                     /translation="MENAAASPAGRFRRHSRRRKCPTLKAQTHTKPYACTCNNPTDTA
                     VFSKPFASFTHPPRDAAATI"
     gene            431..955
                     /gene="nspA"
                     /locus_tag="WJL87_001577"
     CDS             431..955
                     /gene="nspA"
                     /locus_tag="WJL87_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ7799988.1"
                     /translation="MKKALATLIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSIGLGVLTGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSVGVRVKF"
     gene            1091..1480
                     /locus_tag="WJL87_001578"
     CDS             1091..1480
                     /locus_tag="WJL87_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ7799989.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            1558..2388
                     /locus_tag="WJL87_001579"
     CDS             1558..2388
                     /locus_tag="WJL87_001579"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ7799990.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRITKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLKGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            2403..3860
                     /locus_tag="WJL87_001580"
     CDS             2403..3860
                     /locus_tag="WJL87_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ7799991.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(3901..4290)
                     /locus_tag="WJL87_001581"
     CDS             complement(3901..4290)
                     /locus_tag="WJL87_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799992.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKGIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLL"
     gene            complement(4472..4873)
                     /locus_tag="WJL87_001582"
     CDS             complement(4472..4873)
                     /locus_tag="WJL87_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799993.1"
                     /translation="MKIKPLQFSNNSRRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFGF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            complement(5290..5619)
                     /locus_tag="WJL87_001583"
     CDS             complement(5290..5619)
                     /locus_tag="WJL87_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799994.1"
                     /translation="MTDKSKTEKLISSDDKQSVIDGILDMVFNSKAYEVPWISEKLME
                     LSKNKDLDIAGLSLTCFGHLARLHSNIGDYDKVISLLHSKQDDPELQGRAEDALEDIS
                     LFLSENH"
     gene            complement(5645..>6076)
                     /locus_tag="WJL87_001584"
     CDS             complement(5645..>6076)
                     /locus_tag="WJL87_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pretoxin"
                     /protein_id="HEZ7799995.1"
                     /translation="AKQTVQNITFKQQPLKAYNLTVADWHTYFVKGSQAETEGVWVHN
                     DCPYDKGNQRYKDASYHGKNDNSVKSRAPTNGQAALDNSVQVKSTSPRRVGVDKANNE
                     IVVLDKTQTFNNGFAEYHGHVRSWQDLHTDQKNALKKAGLD"
BASE COUNT         1498 a         1762 c         1265 g         1551 t
ORIGIN      
        1 cgcaaacaaa tcggcggccg cccgggaaaa cccgccccga acgcgtccgg aaaatatgct
       61 tatcgatgga aaacgcagcc gcatcccccg ccgggcgttt cagacgacac agccgccgcc
      121 ggaaatgtcc gacgcttaag gcacagacgc acacaaaacc gtatgcctgc acctgcaaca
      181 atccgacaga taccgctgtt ttttccaaac cgtttgcaag tttcacccat ccgccgcgtg
      241 atgccgccgc caccatttaa aggcaacgcg cgggttaacg gctttgccgt cggcaaagca
      301 gccggatacc gctacgtatc ttgaagtatt gaaaatatta cgatgcaaaa agaaaattta
      361 agtataatac agcaggattc tttaacggat tcttaacaat ttttctaact gaccataaag
      421 gaaccaaaat atgaaaaaag cacttgccac actgattgcc ctcgctctcc cggccgccgc
      481 actggcggaa ggcgcatccg gcttttacgt ccaagccgat gccgcacacg caaaagcctc
      541 aagctcttta ggttctgcca aaggcttcag cccgcgcatc tccgcaggct accgcatcaa
      601 cgacctccgc ttcgccgtcg attacacgcg ctacaaaaac tataaagccc catccaccga
      661 tttcaaactt tacagcatcg gcgcgtccgc catttacgac ttcgacaccc aatcgcccgt
      721 caaaccgtat ctcggcgcgc gcttgagcct caaccgcgcc tccgtcgact tgggcggcag
      781 cgacagcttc agccaaacct ccatcggcct cggcgtattg acgggcgtaa gctatgccgt
      841 taccccgaat gtcgatttgg atgccggcta ccgctacaac tacatcggca aagtcaacac
      901 tgtcaaaaac gtccgttccg gcgaactgtc cgtcggcgtg cgcgtcaaat tctgatatgc
      961 gccttattct gcaaaccgcc gagccttcgg cggttttgtt ttctgccacc gcaactacac
     1021 aagccggcgg ttttgtacga taatcccgaa tgctgcggct tctgccgccc tattttttga
     1081 ggaatccgaa atgtccaaaa ccatcatcca caccgacaaa gcccccgccg ccatcggcgc
     1141 gtacagccaa gccgtccgag caggcgacac cgtttacatg agcggtcaaa tccccctcga
     1201 tcccgccacg atgaccgtcg tcggcaacgg cgatttccgc gccgaagcgc gccaagtgtt
     1261 ccaaaacctg caagccgtcg ccgaagcggc aggcggcacg ctggccgaca tcgtcaaact
     1321 caacgcctac ctgaccgact tgggcaattt tgccgtcttc aacgaagtca tggcggaatt
     1381 tatcgccgag cccttccccg cccgcgccgc cgtcggcgtt gcctcgctgc ccaaaggcgt
     1441 gcaggtcgaa gccgaagccg tcctcgtttt gaacgcataa ttccacgttc ggactgcatg
     1501 atatttcaaa accatgccgt ccgaaccgca acactttccc acttcacaac atctgcaatg
     1561 gcacattacg caatcggcga catacaaggc tgtttcgacg aactgaccgc gctgctcggc
     1621 aaaatcggtt tcaaccacgg cacggacacc ctctggctga cgggcgacat cgtcaaccgc
     1681 ggcccgaaat ccctcgaaac gctgcaattc tgcatccggc acgaaaacag cgtgcaaatc
     1741 gtcctcggca accacgacct gcacctgctc gctgtcggct gcggcgaagg tgcgcccaaa
     1801 cgcagcgaca caatcgaacc catactcaaa caccccgacg gaaaaaaaat gctcgactgg
     1861 ctgcgtgcgc aaccgctgtt gatacgcgag ggcagccgcg tgatggtaca cgccggcatc
     1921 ctgccgcaat ggcgcataac caaagccgaa tcgctcgcta gagaagccga agccgaactg
     1981 cgcggcaaaa aatacgtcaa attcttctcc aaaatgtacg gcaacaaacc ggccgcgtgg
     2041 gacgaaggtt tgaaaggcta tgcccgcctg cgcttcatcg tcaacgcctt cacgcggatg
     2101 cgcgccctga cctttaaaaa cgaactggat ttcgactaca aatccacagt gaaaaaaatg
     2161 ccgccttacc tgcgcccgtg gttcaaagcc cccgaccggc aaaacctcga ccgcaccatc
     2221 atcttcggac actggtcctc gctgggctac acgaatgccg acaacgtcat ctcgctggac
     2281 accggcgcgc tgtggggcgg acagctgacc gccgtcaacc tcgaaacgga agaaattacc
     2341 caagtccaag ccgccggcgg catagactgg aaaagcttcg caaaataacg gaccgcccca
     2401 ctatgcacaa aatcctgccc atcgcccacg tcctctcccg actgggtatg ctgttttcct
     2461 ttatcctgct gatacccgcc gccctctcct acgccttttc ggacggcgcg tacaccgcct
     2521 tcgccaccac cgcgaccgtt accctttccg gctcgtgcat cgtccggctc gccaccctcc
     2581 ggttcaggcg cgaactgcgc ccgcgcgacg gctttaccct cgtcctgatg ttgtggctgg
     2641 cgtttgccgc tatggctgcg atgccgatgt acctgtattt cccgaatatg ggctttaccg
     2701 acgcattttt tgaatcgatg tcgggactga ccaccaccgg cgcgaccgtc atcccccacg
     2761 tcgacgggct cgccccctcc gtcaactttt ggcggcatat gctcaactgg ctgggcggga
     2821 tgggcatcat cgtccttgcc gtcgccatcc tgcctatgct cggcgtaggc ggcacgcagt
     2881 tattcaaagc cgaaatcccc ggcattgaca aagaaagcaa aatgtcgccg cgcatttccc
     2941 aagtggcgaa aaaactttgg ttcggctaca ccctgatcac catcctcgcg gcagcctgcc
     3001 tgcattttgc cgggatgggc tggttcgatg ccgtctgtca cgcgatggcg accctctcgc
     3061 tgggcgggtt ttccacccac gatgccagca tagcttatta caactccccc ctcatcgaag
     3121 cggtcatcat cgttttcacc attgtcggcg gcatcaattt tgccaaccat ttcgccgccc
     3181 tcaacagccg cagcctcaaa acctattgga aagacgaaga atgccggacg atgctgctgc
     3241 tgctctccgg cagcatcctt gccgccgccc tgtacctgtg gcacaccggc tattacgccg
     3301 gttttaccga atccctgcgc tacaccgcct tcaacttcgt ctccatcgga ctggcaaacg
     3361 ggctggccaa caccgacttc gcacaatggc cgctcctgat ttccctgtgg atgtttttcc
     3421 tcgccaacat cctcgccaac tccggctcga ccggcggcgg catcaaaacc atacgcgccc
     3481 tcgtcctgtt caaattcagc ctgcgcgaaa tgatggtgct gctgcacccc aaagccgtcc
     3541 gcaccgtcaa aatcagcggc aaggccatac ccgaccgcct cgcgctgacc gttatgtcct
     3601 tcatcttcat ctactttatg accgtcgtcc tcttcagctt cctgctgatg gcgagcggta
     3661 tggaatttac caccgccttc accgccgtca tcgcctgcat caccaacgcc ggccccggcc
     3721 tgggcgaagt cgggcccgcc ggcaattacg ccggtttgga cgtgatgcag aaatggatct
     3781 gcgttaccgc catgctcttg ggcaggctgg aaatcttcac cgtcttcatc ctcttcaccc
     3841 ccgcctactg gaagaaataa aaaatgccgt ctgaaaactt ttcagacggc atcttgtcct
     3901 ttaaagaagc ggaagctcta caaaaggaaa gaaatgctga aacctctgat aaatttcagg
     3961 atatttctct aaggctttta atgctttttc ttttgaaata ggcggatcat agacatctat
     4021 cccccttaag aaggcaatgc ccgttaatgc atttttttga tcatttgaaa tgtaaccata
     4081 attaacgact ccaattacat cataaatttc ccctataccc ttatcaaaat caatcaaatc
     4141 aaaaatatac aatggaattg tatcaatagg agtatctctg acaactatat ccaaccataa
     4201 tttaaattca gaaagattaa tagcatcata gaacatacag actattgcaa atcctaaatc
     4261 ttcacaattt tctttactaa ttttccacat aattatttac ctttatccat tgcttcccaa
     4321 ccgatacatc cggtatttta gcttctcctt tgccgtcaaa cactcctttg gttacctgct
     4381 ttgtgtcaaa aataccgtct gaaagccgaa tatcgtttca gacggcattt tgattgttgg
     4441 gtttttaatc agagctttgt ttgataattt gttattttga ataaaataca ggttctgtcg
     4501 agctgataac ttctattaca gaatcgctaa aaaagaaaat gtaagcctta taatatttct
     4561 tttcaaaaaa accaaaactt tcttctttca accagtccat ataactgtca tctattatct
     4621 cgaaaaaata atttatagca ggatttctat cccaatcatt tttttcttta ttttcaaata
     4681 aatattctga aaattttact ttataagatt catctgttat ttgaaaatga attatattgt
     4741 caataaatat ttcaatattt aattcataat ttcggtcgga tgccaaaatg ttcacatctt
     4801 gttcggtaac acaaattttg tctacgtaaa atctgcgact attgttagaa aattgtaatg
     4861 gtttaatctt cacaatcgtt ttccatatct aatttttgtt ttcttattgc cctgccagat
     4921 aaagcaggtc tgcctccgta ctgtctatcc tcacatttac tttcctgcca tcggctttta
     4981 tctccccggt tttaccgttt ttgccaactt tgcggctttg gcggcggcaa tgttgaaggc
     5041 ggcttcgacg gtttcggcgg cattggggtt gacccgtact catccgcaaa cggcaacacg
     5101 acggacggtg tttgcttttc agccgtctgc cgttttgacc aaggtgctgc cgtggaagga
     5161 gcaagtgtag gatttttgaa gaactcagtt ctcaatatcc tatttcaaac gaaggaaatg
     5221 ccgtctgaaa attaaacacg gtttcagacg gcattttgat tgttgggttt tcaacccgac
     5281 ctacggttcc taatgatttt cagataaaaa taaagaaata tcttctaacg catcttcagc
     5341 cctaccttga agctctggat catcttgctt tgaatgtagt aaagaaataa ctttatcgta
     5401 atcaccgata tttgaatgta gcctggcgag atgtccgaaa caggttagcg ataatccggc
     5461 aatatccaag tctttatttt tcgataattc catcaatttc tcagaaatcc acggtacttc
     5521 atatgctttg gaattaaata ccatatcaag aatgccatct ataacacttt gtttatcatc
     5581 agaagaaatc aacttttctg ttttactttt atcagtcatt ttttaatttt tccttttgaa
     5641 ttaactaatc caatcctgct ttttttaaag catttttctg atcggtatgc aaatcttgcc
     5701 aacttctgac atgcccgtga tattccgcaa aaccgttatt aaaagtttga gttttgtcta
     5761 atacaacgat ttcattattg gctttatcaa ccccaactct tcgaggagaa gttgatttaa
     5821 cttgaacgga attatcaaga gctgcttgtc cgtttgttgg tgctctactt ttcacagaat
     5881 tatcattttt gccatgataa gaagcgtctt tatatcgttg gttgccttta tcatacggac
     5941 aatcattatg aacccaaacc ccttccgttt ccgcctgact gcccttgacg aagtaggtat
     6001 gccaatcggc gacggtcaga ttgtaggctt tgagcggctg ctgtttgaag gtaatgtttt
     6061 gaaccgtctg ttttgc
//
LOCUS       DAWKST010000122         5976 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.122, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000122 DAWKST010000000
VERSION     DAWKST010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.122"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            965..1201
                     /locus_tag="WJL87_001585"
     CDS             965..1201
                     /locus_tag="WJL87_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ7799996.1"
                     /translation="MKELHTSELVEVSGGKFHISAQGGGNLGKKDMVAVGKIGASYSP
                     NNSGVEFSVSKQFGYVQGFGVQFSKPTFGFSKKW"
     gene            2241..2378
                     /locus_tag="WJL87_001586"
     CDS             2241..2378
                     /locus_tag="WJL87_001586"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7799997.1"
                     /translation="MNTETIYATVFCILAATISGLLVKFNVIKIETSINSKFMLLGIS
                     I"
     gene            2423..3688
                     /locus_tag="WJL87_001587"
     CDS             2423..3688
                     /locus_tag="WJL87_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ7799998.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLPTMGVVRVYSSDIGTITHKFVEDGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQIRLAEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRSRLESLKREQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSGYISTINV
                     DIGQQVEPSKLLLSIVPEQTELVANLYIPSKAVGFIKPKDKVVLRYQAYPYQKFGHAT
                     GEIISVARTALGKQELSGLGIIFTNPTLLNEPAYLVKVKLEKQTIKAYGENKPLQIGM
                     ILEADILHERKKLYEWVLDPLYSISGKIN"
     gene            3691..5778
                     /locus_tag="WJL87_001588"
     CDS             3691..5778
                     /locus_tag="WJL87_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEZ7799999.1"
                     /translation="MDYLSRLSFGFNKKLPVILQTEVAECGLACLTSILSYYGFHTDL
                     RTLRQKYTLSLKGANLADIMRFGNEMNLTPRALRLELDELSNLQLPCILHWNLNHFVV
                     LCSISKDSIVIMDPAVGMRKIKMDEVSQKFTGIALELFPNTHFEEKKETKKIKILSLL
                     RGISGLKRSLIQMLILAISLEVFALVSPFFMQWVIDHVIVTADKNLLLTLTLGFGLLA
                     ILQQLISLLQAWVGMHLSTTLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSIDH
                     IQETLTSTFFVLVLNSLMAIFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLRNA
                     TEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLSAL
                     FEFSNKLLFSMENIIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYIQI
                     KMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFENI
                     NLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGLSG
                     IVRQDDVLFAGSIGENISFFDESPNMELIEQCAKMAQIHDDILKMPMGYETLIGDMGN
                     ILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAHRQ
                     ETIQSADKILNLG"
BASE COUNT         1978 a         1020 c         1067 g         1911 t
ORIGIN      
        1 aaaattccat gtttgtgtac tgaaatgctt caaaacacca aaccaagttt cgttttctaa
       61 aatacgaaac cattactgct gcctaaattt ttttggattg ctaaattatg gcagtatgat
      121 tttggatttt aaattgaaag gcaagaaaaa tgtcaaaaaa tgatgtagtt aaagtaattg
      181 gtatattccc cctattgtcc gaacaataga gcagacttcc cggcaggtca aattgtttta
      241 aaacacggcg aacacaaaaa ggttaatcac ggctttggtg tgattcacat tttggcagaa
      301 cacaaagcag atttaacgcg gtttggatta agccacgatg aaacaggtgt aaccctttta
      361 tgtggaaatg atttttcaat caggcgcaaa aatttttagt gaatttagtg atgtaagggg
      421 caatcatcgt cctatgattg tctattctcg gattgggaca gtcgttttag aacgcaaaga
      481 agttgagggg gaaagtgtgt attcagttgt taccgcgttt gggcgaacaa gccctcgtgg
      541 tacacaaatt ggtacaatgc cataaaaaga caaaagcacc caataatttc aggtgctttg
      601 tgtagtagcc gcagcctctc cttagcttga tagggtcgga taccaatctg acgattgagc
      661 tactgaaatt ggttagcgac aaccaacctg cccacatcag aacgcccgtt cgctggtttg
      721 tacgtcctga aaaagctctt gcattaagtt aatcataatg ggaaatttaa attttttcaa
      781 tgcttactta aacaaaagcc ccactccacc attaggagtt tctttttcag tatacaagta
      841 aatattttta aaatattgat ttaatttaaa ataaaatact tgcaaaaaaa aagtattaaa
      901 ttaaacttga gaaaggttaa ttctgattta catttccaac catacttctt tacaggagaa
      961 aatcatgaaa gagttacaca cctctgaatt agttgaagtg tcaggtggca aattccatat
     1021 ctctgcacag ggtggcggca acctaggtaa aaaagatatg gttgctgttg gtaaaattgg
     1081 tgcttcctat tcccctaaca atagtggagt agagttttct gttagcaagc aatttggata
     1141 tgtacaaggt tttggtgtac agttttcgaa acctactttt ggttttagta aaaaatggta
     1201 agattttttg ttttatcctt tctgacatta ataaatctat gctcattaag cgcatgcaat
     1261 agccacttta caggaaatat caatccatta ggtactcaca ataaagttgc taatcccagt
     1321 tgtgccaata gtgccaatag tcatatcaga caacccagta gtaaaaacta tgatccaact
     1381 gaatatagtg cttggttaca gcatatgcat gattgcaaat aatgagtaac gatgaaaatt
     1441 tacttttttc tcaaccacac ttaacaaagg tgaatattat gcaagttttg actttgaatg
     1501 aaatttaaca agtttctggt gctgcttgta actggcgtga tttctcaaaa aataccattg
     1561 gtagtgcatt aggtggagca gctggtgggg caattgttgg ttcatttgca ggtggtattg
     1621 gtgctattcc aggtgcgaaa ttcggagcta ttggtggtgc aatcactggt gctgtacaat
     1681 atggaagcac ttgttggtgg taatattcct taataaaact agggtatttt gatattttct
     1741 attcaaaata ccctagtttt tcataagaac ttaaatacaa aaaggaacaa ataatgaaaa
     1801 aatatagtga ttattttaaa tatttaatct ttttttgatt ttactcccaa caaattatct
     1861 cgtatctcat tatgtggtac aaacctcaat gagtatgtta agcattttaa gttcttctat
     1921 aataacaact tgcctttttt ttgtttttac aaatttatcc caatcaaaga aacataaata
     1981 agtacacatg tctaacaatc actcattttt cagaccagaa gtctttgtag ctcaacggaa
     2041 caagtggaca ggaccagtag gctgggttgg cgcaattgga gctggtattt tctctgctgc
     2101 tggcggatac aatatcggtc gtggcatgat gaagccataa gataattaca tcattaagga
     2161 aaaggtaatt tcagttacag caatatgtat tgaagttacc tttttctatt tagattgaac
     2221 aattttgaaa gagaaaaatt atgaatactg aaaccattta cgccactgtc ttttgcattt
     2281 tagctgcaac catttctgga ttattggtta aatttaatgt aattaaaata gaaacatcaa
     2341 tcaatagcaa atttatgtta ttaggcataa gtatttaatt attggtgttt ttctatccat
     2401 tttttttaag aaataataat aaatgtccca cttattccga aaagaagtct ttgtagccca
     2461 acaaaataag tggacaggtc aggttatctt gacccgtcca ttctcttttt tatttctgac
     2521 tttttgcgct tttctcattg ctctgtgtat cattatcttt ttgatttttg gtagctatac
     2581 caataaaaca accgttgaag gtcaattact tccaactatg ggggtggttc gtgtttactc
     2641 ttccgatatc ggcacgatta cgcataaatt tgttgaagat ggtaactttg tcaaagctgg
     2701 cgaaccattg ttcaaacttt ccacatcgcg ttttggcgaa aaaggaaacg tacaagccaa
     2761 attggcagca gaagccaacc ttaaaaaaac tttggcatta caagaattgg aacgtttaaa
     2821 gcgcattcat caaaatgagc aaaaaaatgt tcataacaac attcatcgtt taaacaatca
     2881 attagagaat attaaacagc aaattacagg gcaaaatcgt caaattcgtt tagcggaaaa
     2941 aacccttaac aagaacaagt ttttagccag tcaaggcgca gtatcccaac aagataagat
     3001 gaccgccgaa agccatttat tggaacaacg ctcacgtttg gagagcctaa aacgtgaaca
     3061 aaataatgca atcagggaac ttgatgaaca gaaaatcaca ttaagcagcc tgcctgaacg
     3121 ccataaaacc gaattgagcc aactcaaccg tgcgattacg gaaatgaacc aagaaatttt
     3181 ggattttgat ttgaaatccg aacaaaccat acgagctagt aaatcaggtt atatttcaac
     3241 aattaatgtt gatatagggc aacaagttga accgtctaaa ttgctgttaa gcattgtccc
     3301 tgaacaaact gaattggtcg ccaatcttta catacccagt aaagctgttg gttttattaa
     3361 accgaaagat aaagttgttt tacgttacca agcgtaccct taccaaaaat ttggacatgc
     3421 cacaggagaa attatttcag ttgccagaac tgctctcggt aaacaagagc tatcaggttt
     3481 aggtatcatt ttcactaacc caaccttatt aaatgaacct gcctatcttg tgaaagttaa
     3541 attggaaaaa caaacgatta aagcatacgg agaaaacaag ccgcttcaaa ttggcatgat
     3601 tttagaagca gatattctcc atgaacgaaa aaaattgtac gaatgggtac ttgacccact
     3661 ttacagcatt tcaggaaaaa tcaattaaaa atggattatt tatcaagact gtcctttgga
     3721 tttaacaaaa agctacctgt cattctgcaa acagaagttg ctgaatgtgg tttagcatgc
     3781 ctgacatcca tcttgtccta ttatggcttt cacactgatt taagaacgtt acgccaaaaa
     3841 tacaccctgt cattaaaggg cgcaaatctt gcagacatca tgagatttgg caatgaaatg
     3901 aatttaacgc cacgagcttt gcgtttagag ttagatgagc tgtcaaattt acaactaccc
     3961 tgcattctcc attggaactt aaaccatttt gttgtacttt gttccatttc caaagacagt
     4021 atcgtcatta tggaccctgc tgtcggtatg cgaaaaatca aaatggacga agtttcacaa
     4081 aaattcacag ggattgccct agaattattc cccaataccc attttgaaga gaaaaaagaa
     4141 acaaagaaaa tcaaaatatt atctctatta agggggattt caggcttaaa acgctcttta
     4201 attcaaatgc ttatattagc tatttctttg gaagtctttg cattggttag tccattcttt
     4261 atgcaatggg taatagacca tgtcattgta actgctgata aaaatttatt actgaccctt
     4321 actttgggat ttggtttact ggctatcctg caacagttaa ttagcctgtt acaagcatgg
     4381 gtaggtatgc acctatctac aactcttaat ttacaatgga aagccaatat atttaaaagg
     4441 ttacttgact tacctaatga ctatttcagt aaacgacatt taggagatgt gatttcaaga
     4501 tttggttcaa tagatcatat ccaagaaaca ctaacttcta ctttttttgt tttagtttta
     4561 aatagcttaa tggctatttt tactttcgtg ttaatgacaa tttacagcac tcaattatcg
     4621 ctgattgttc ttttaacact tgttttgtac atactaattc gttggcttgc atattaccca
     4681 ttaagaaatg caacagaaga aaatattgtt catgaagcca aacaaaactc atatttcatg
     4741 gaaaccattc gtggtatcca atcagttaaa ttatttgata aacattatca aagacatggc
     4801 acttggatga gcctatttgt gaatacagtc aataccaagc tgacaacaga taaactctct
     4861 gctttatttg aattttcaaa taaactgttg tttagcatgg aaaatattat cataatttat
     4921 cttggtgcaa gcgcaatttt agatggttca tttacagtcg gtgttctgat ggcttttttg
     4981 gcttataaag ggcaatttga aagcagaaca gcttctctcg ttgaccaata catccaaatc
     5041 aaaatgttag ggcttcatgc tgaacgtttg gctgacatta ctttaaatga aacagaaact
     5101 gaaattatta agtataatca tatacctaaa ttagataatg aacaactggt tcttaaagtt
     5161 gaaaacgtct cattcagata tgctgataat gagccatatc tttttgaaaa cattaatttg
     5221 gaatttaaag ataatgaagc agttgtttta acaggacaat ctggtcgtgg gaagtccact
     5281 ttgttaaaca ttttaacagg tagcctaaaa cctgaaactg gtacagttag tattaatggg
     5341 catgatatat atcaagtttc tccatccttt attaggggat tgagcgggat tgttcgccaa
     5401 gatgatgtcc tttttgcagg ttctattggg gaaaatattt cattttttga tgaaagccca
     5461 aatatggagc tcattgaaca atgtgcaaaa atggcacaaa tacatgacga tatacttaaa
     5521 atgccaatgg gctatgagac cttgattggc gatatgggaa atatcttatc aggtggacaa
     5581 aagcagagag ttatcttggc tcgtgcattg tataaacgac ccaaaattct atttttagac
     5641 gaagcaagta gccatttaga tgtagaaaat gaacaaaaaa ttaaccataa cctaaaaagt
     5701 cttggtatta tgaaaataat ggttgcacac cgccaagaaa caattcaatc ggcagataaa
     5761 attctgaatt taggttgaac agaacaagac ttcatttttc tttaacaaaa agtgaagtct
     5821 tttttcaaat aatttaatag aatacatgaa aatagcggtt taacgttcca tttcccaatc
     5881 atcacgactg gctttgtgtt ttggcgattt ttcagtttcc tttttctgtt gaatttgttg
     5941 ttttttctgc tcttgttccc atttttgggc taattt
//
LOCUS       DAWKST010000123         5933 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.123, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000123 DAWKST010000000
VERSION     DAWKST010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.123"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            59..1597
                     /gene="murJ"
                     /locus_tag="WJL87_001589"
     CDS             59..1597
                     /gene="murJ"
                     /locus_tag="WJL87_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ7800000.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPS
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLASGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFYLLRRHGIYQPGKGWAAFLAKMLL
                     SLAVMGGGLWAAQACLPFEWVRAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
     gene            1730..2686
                     /locus_tag="WJL87_001590"
     CDS             1730..2686
                     /locus_tag="WJL87_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEZ7800001.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRPAPAYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSGSHYAVELMLAVRDRGWNGAVVHFRSCGGIA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGERGENALPQAAA
                     VISAPVDAEAAGRRFDSGITRLLYTRYFLRTLIPKARSLHGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAANDPFLPPEALPTPDEVSEAV
                     TLFQPAHGGHAGFVGSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            2737..3519
                     /gene="trpC"
                     /locus_tag="WJL87_001591"
     CDS             2737..3519
                     /gene="trpC"
                     /locus_tag="WJL87_001591"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ7800002.1"
                     /translation="MTDILNKILTAKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIGS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQEQLERFEAVA
                     HELGMTVLLELHDESELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMQSRGVHTFLIGETFMRADNIEAEVGKLF"
     gene            3585..5885
                     /gene="recQ"
                     /locus_tag="WJL87_001592"
     CDS             3585..5885
                     /gene="recQ"
                     /locus_tag="WJL87_001592"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ7800003.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGGSLTVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADEAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGM
                     LAERYPNVPRIALTATADAATRADIKHYLHLDDAPEFISSFDRPNIYYQVIEKNNGKK
                     QLLEFIRKEMAGQSGIVYCLSRKKVEDVAQFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEESEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGAELSDKEWRSVIRQCISLGYLTVNIARYQALQLTEAAKKVLKGETEVMLRPLK
                     RGKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QNLNGLHDIYGLGEAKTERFGHGILEVCRNAAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLADTPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDETAKHKRRLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLERNAV"
BASE COUNT         1294 a         1763 c         1618 g         1258 t
ORIGIN      
        1 tatcaaagtc tgatcctgcc gccctaaagg gcggggtttc aaccgaaaag gaaatacgat
       61 gaatatgctg ggagctttgg caaaagtcgg cagcctgacg atggtgtcgc gcgttttggg
      121 atttgtgcgc gatacggtca ttgcgcgcgc attcggcgcg ggcatggcga cggatgcgtt
      181 ttttgtcgcg ttcaaactgc ccaacctgct tcgccgcgtg tttgcggagg gggcgtttgc
      241 ccaagcgttt gtgccgattt tggcggaata taaggaaacg cgttctaaag aggcgacgga
      301 ggcttttatc cgccatgtgg cggggatgct gtcgtttgtg ctggtcatcg ttaccgcgct
      361 gggcatactt gccgcgcctt gggtgattta tgtttccgca cccggttttg ccaaagatgc
      421 cgacaaattt cagctctcca tcgatttgct gcggattacg ttcccctata tcttattgat
      481 ttcactttcc tcttttgtcg gctcggtact caattcctat cataaattca gcattccggc
      541 gtttacgccc acgtttctga acgtgtcgtt tatcgtattc gcgctgtttt tcgtgccata
      601 tttcgatcct cccgttaccg cactggcatg ggcggttttt gtcggcggca ttttacaact
      661 cggcttccaa ctgccctggc tggcgaaact gggttttttg aaactgccca aactgaattt
      721 caaagatgcg gcggtcaacc gcgtcatgaa acaaatggca cctgcgattt tgggcgtgag
      781 cgtggcgcag atttctttgg tgatcaacac gattttcgcg tcttatctgc aatcgggcag
      841 cgtttcatgg atgtattacg ccgaccgcat gatggagctg cccagcggcg tgctgggggc
      901 ggcactcggt acgattttgc tgccgacttt gtccaaacac tcggcaaacc aagatacgga
      961 acagttttcc gccctgctcg actggggttt gcgcctgtgt atgctgctga cgctgccggc
     1021 ggcggtcgga ctggcggtgt tgtcgttccc gctggtggca accttgttca tgtaccgcga
     1081 attcacgctg tttgacgcgc agatgacgca acatgcgctg attgcctatt ctttcggttt
     1141 aatcggtttg atcatgatta aagtgttggc atccggcttt tatgcgcggc aaaacatcaa
     1201 aacgcccgtc aaaatcgcca tcttcacgct catctgcacg cagttgatga accttgcctt
     1261 tatcggccca ctgaaacacg tcggactttc gcttgccatc ggtctgggcg cgtgtatcaa
     1321 tgccggattg ttgttttacc tgttgcgcag acacggtatt taccaacctg gcaagggttg
     1381 ggcggcgttc ttagcaaaaa tgctgctctc gctcgccgta atgggcggcg gactgtgggc
     1441 ggcgcaggct tgcctgccgt ttgaatgggt acgcgccggc ggaatgcgga aagcggggca
     1501 gctctgcatc ctgattgccg tcggcggcgg actgtatttc gcatctctgg cggctttggg
     1561 cttccgtccg cgccatttca aacgggtgga aagctgaccg atgccgatat cctttttcgg
     1621 aaaacgcttt ttccgcgcct atattcgggc gcgcatcccc cacgtccgca ccagccggac
     1681 gggctgccgc atcatcaatg ccgtctgaaa acaagaaaac cgatacctta tgattttaac
     1741 accgccggac acgccctttt tcctccgcaa cggcaatgcc gacacgattg ccgccaaatt
     1801 cctgcaacgc cccgcacctg cataccgccg cgagctgctt ccagacagca cgggtaaaac
     1861 caaagtcgcc tacgactttt cagacggcat ttcgcccgac gcaccgctgg tcgtgctgtt
     1921 tcacggtttg gaaggaagca gtggcagcca ttacgcggtc gaactgatgc tcgcggtacg
     1981 cgatcgcggt tggaacggcg cagtcgtcca tttccgcagc tgcggcggca ttgccaacac
     2041 cgctccggtg ttctaccact tgggcgatac cgccgaaatc gcctttactt tggacacgct
     2101 tgccgcgcgt taccgtgaaa tatacgccgt cggcgtatcg ctgggcggca acgcgctggc
     2161 aaaatatttg ggcgaacggg gcgaaaacgc gctgccgcaa gccgctgccg tcatctccgc
     2221 acccgtcgat gcagaggcgg caggcagacg cttcgacagc ggcatcacgc ggctgctcta
     2281 cacgcgctac ttcctccgca ccctgatacc caaagcacgg tcgctccacg gttttcagac
     2341 ggcatttgcc gcagggtgca aaacactggg cgagtttgac gaccgcttca ccgcaccgct
     2401 gcacggcttt gccgaccggc acgactacta ccgccaaact tcctgcaaac cgctgctcaa
     2461 acacgttgcc aaaccgctgc tcctgctcaa tgccgccaac gaccccttcc tgccgcccga
     2521 agcgctgccg acaccggacg aagtgtccga agccgttacc ctgttccagc cggcacacgg
     2581 cggacacgcc ggctttgtcg gcagcaccgg cggcaggctg cacctgcaat ggctgccgca
     2641 gaccgtcctg tcctatttcg acagcttccg cacaaacagg cggtaacggt ttgatgctaa
     2701 tattccccct ttccccggac aaatacggaa cacgacatga ccgacatcct caataaaatc
     2761 cttaccgcca aggcacagga agttgccgct caaaaagccg ccgtcaacgc cgaacacatc
     2821 cgcgcacttg ccgcagaagc cgcgcccgtc cgcagcttca tcggttcgat acgcggcaaa
     2881 caccgcctaa acctgcccgc cgtcattgcc gaaatcaaaa aggcaagccc gagcaaaggc
     2941 ctgatccgtc cggacttccg accggccgag attgcacgcg cctatgaaaa cgccggggcg
     3001 gcgtgtttgt ccgtactgac cgacgaaccc tatttccaag gttcgcccga atacctcaaa
     3061 caggcgcgcg aagccgtatc gctgcccgtg ctgcgcaaag acttcatcat cgacgaatat
     3121 caggtttatc aggcgcgcgc atggggggcg gatgccgtcc tgctgattgc cgcagcactg
     3181 gaacaggaac aattggaacg ctttgaagcg gtggcgcacg aattgggcat gaccgtcctg
     3241 ctcgagctgc acgatgaaag cgaattggaa aaatgccgca acctgaccac gccgttgtgg
     3301 ggtgtaaaca accgcaacct gaggactttt gaagtctccc tcgaccaaac cctgtcgctg
     3361 ctgcccgcgc tggaaggcaa aaccgtcgtt accgaaagcg gcattacagg caaggcggat
     3421 gtggaattta tgcaatcgcg cggcgtgcat accttcctga tcggcgaaac gtttatgcgt
     3481 gccgacaata ttgaggcgga agtaggcaaa ctcttctaaa tcccgatttc agacggcatt
     3541 ccctttcttt aaccgacaca ccctttaccg gacacggaac catcatgcac cgaccgaccg
     3601 ccaaacagat tctgcacgaa gtatttggtt atcccgaatt tcgcggcagg caggaggctg
     3661 tcatcaatac tttggcaggc ggcgggagtt tgacggtgct gatgccgacg ggcgggggca
     3721 agtctttgtg ttaccagatt cccgcgctga tgcgcgaagg cgtggcggtt gtcgtatcgc
     3781 cgctgattgc gctgatgaac gaccaagtgg ccagcctgca tgtggccggg attgaagcag
     3841 cggcagtcaa cagcggcaca tcggcagatg aggcgcgcga gattgccgac aagcttgccc
     3901 aaggccgtct gaaactgctt tatgtcgcgc cggaacgctt ggttaccgat cgctttttgc
     3961 gttttctcga ccaacaaacc gtcagcctgt ttgccattga tgaggcgcat tgcgtcagcc
     4021 agtggggaca cgatttccgc cccgaatacc aacagctcgg catgcttgcc gaacgctatc
     4081 cgaacgtccc gcgcatcgcc ctgaccgcca ccgccgatgc cgccacgcgc gccgacatca
     4141 agcattatct gcacttggac gatgcgcccg aatttatttc cagcttcgac cgcccgaata
     4201 tttattatca ggttatcgaa aaaaacaacg gcaaaaaaca actgcttgaa tttatccgca
     4261 aagaaatggc ggggcaaagc ggcattgtgt attgcctaag ccgcaaaaag gttgaagatg
     4321 tggcgcagtt tttgcgtgaa aacggattaa acgcgattcc gtatcatgct ggtttgagca
     4381 tggacgtgcg cgaggaaaac caacgccgct ttacgcatga agacaatatt atcgtggtgg
     4441 cgaccgtggc gttcggcatg ggcatagaca aacccgacgt gcgctttgtc gcccatctcg
     4501 atatgcccca gagtgtcgaa catttctatc aggaatcggg gcgtgccggc agggacgggc
     4561 tgcctgccgc gagctggctg tgttacggct tgaacgattg ggtgttgctg cgcgaacgga
     4621 ttgccgaagg caacagcgat gaggtgcaaa agcaaatcga aatgcaaaaa ctcgatgcca
     4681 tgctttccgt ctgcgaaacc gccgcctgcc gccgcgtact gttgctcaaa cattttggcg
     4741 aagaatccga accctgcggc cattgcgaca actgcctgca tccgcctgtg cggtttgacg
     4801 gcacggtgtt ggtacaaaaa ttactcagct gcgtgtaccg cgccggacaa cgttttgccg
     4861 ccggttacat caccaacctt ttgcgcggta aaagcgacga ttggatacgc ggcaaccggc
     4921 acgaacaact gtctaccttc ggcatcggtg cggagttgtc cgacaaagaa tggcgcagcg
     4981 tcatccgcca gtgcatcagc ctcggctacc tcaccgtcaa cattgcccga tatcaggcat
     5041 tgcaactgac cgaagccgcc aaaaaagtcc tcaaaggcga aaccgaagtg atgctgcgtc
     5101 cgctcaagcg cggcaagccc gccgcccgca ccctcaaaga caactggctg cgtaccgaac
     5161 gcgaagaacg cctgtggcag gcattgcgcg tttggcgtat gaaacaggca gaagccgaag
     5221 gcatccccgc ctatatgatt ttcggcgaca aaaccctgcg cgaccttgtc gaaaaaatgc
     5281 cgcaaaacct caacgggctg cacgacatct acggcttggg cgaagccaaa accgaacgtt
     5341 tcggacacgg catactcgaa gtctgccgca acgccgccgg cttcagccgc gacgcggtca
     5401 tccgtccgca aaccgaacgc gaacaacaac tgcgtcaaaa actcgaggcc tggcggtatg
     5461 aacaggcaag ggcggaaaac tgcgccctgc atgccgtcct ctccgacgaa agccttgccg
     5521 atatgcttgc cgatacgccc gaaaccgaaa ccgacctcga aggcgtacac ggcttgggca
     5581 gcgtacgcgc cgccaaatac ggacgggaca tcctcgccgt ctgccgtccg ttttcagacg
     5641 gcatcgatga aaccgccaaa cacaaacgcc gcctgatgcg cgccctgatc caatggtgca
     5701 acgaaacggc aaaacacgaa cagtccgaac cctaccgcat tctcagcaaa gccgccctac
     5761 gcgccattgc cgccaaacag ccggaaggtt tggcggagct tgccgccgta tatggcgtag
     5821 gcgaagaaaa agccgcacgt tacggtgcgg cggtgttggc ggtattggaa cgaaatgccg
     5881 tctgaaaacc gttaccaagt ttcagacggc attgcctcta tttaaaaatt cct
//
LOCUS       DAWKST010000124         5917 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.124, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000124 DAWKST010000000
VERSION     DAWKST010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.124"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            310..1257
                     /locus_tag="WJL87_001593"
     CDS             310..1257
                     /locus_tag="WJL87_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ7800004.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMTYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAA
                     GMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVALHNGIGYLLGFFAAKWTGLPYDAQKTLAIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(1409..2533)
                     /gene="rluD"
                     /locus_tag="WJL87_001594"
     CDS             complement(1409..2533)
                     /gene="rluD"
                     /locus_tag="WJL87_001594"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ7800005.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTLWIKEGAVIVNDKPSQPKDKMIGGEQIRVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWRGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLTHSYIECSLETGRTHQIRVHMREANHPLAGDPVYG
                     NPRHPCSDPVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            2532..3335
                     /locus_tag="WJL87_001595"
     CDS             2532..3335
                     /locus_tag="WJL87_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ7800006.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(3393..5612)
                     /locus_tag="WJL87_001596"
     CDS             complement(3393..5612)
                     /locus_tag="WJL87_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ7800007.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTAVRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVAAAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRIRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         1432 a         1865 c         1427 g         1193 t
ORIGIN      
        1 acggcttcgt cgccttgtcc tgatttttgt taatccacta tatgtttcaa caggggatgg
       61 cacatcaagc accgcactgc gtgttgcccg aacgagacta ccctccgatt agattctatc
      121 gctataaaca gacgggtttc aaccgaaaag gaatggggat aaagtccatt tctgacacct
      181 ctcgggcgat gccgtctgaa aaccaatctc cactttcaga cggcattgtt taggcacggt
      241 cattttgcaa attaaggtta aggcgtgtat gcttgccttc ccttaaaaac aaacacgcaa
      301 cgcattatta tgaatatcct cagtaaaatc agcagcttta tcggaaaaac attttccctc
      361 tgggccgcgc tctttgccgc cgccgctttt ttcgcgcccg acaccttcaa atgggcgggg
      421 ccttatattc cttggctgtt gggcattatt atgttcggta tgggtttgac gctcaaacct
      481 tccgacttcg atattttgtt caaacatccc aaagccgtca tcatcggcgt aatcgcacaa
      541 ttcgccatta tgccggcaac cgcctggctg ctgtccaaac tgttgaacct gcctgccgaa
      601 atcgcggtcg gcgtgatttt ggtcggctgc tgcccgggcg gtacggcttc caatgtgatg
      661 acctatctgg cgcgtggcaa tgtggctttg tcggttgccg ttacgtctgt ttccaccctg
      721 atttccccat tgctgactcc cgccatcttc ctgatgcttg ccggtgaaat gctggaaatc
      781 caagcggccg gtatgttgat gtccatcgtc aaaatggttt tgctccccat tgttttgggt
      841 ttgattgtcc ataaggtttt gggcagtaaa accgaaaagc tgaccgatgc gctaccgctg
      901 gtttccgttg ccgccatcgt gctgattatc ggcgcggttg tcggggcaag caaaggcaag
      961 attatggaaa gcggcctgct gatttttgcc gttgtcgcac tccacaacgg catcggctac
     1021 ctgctcggct tctttgccgc caaatggacc ggcctgcctt atgatgcaca aaaaacgctg
     1081 gccatcgaag tcggtatgca aaactcgggg ctggccgccg cgcttgccgc cgcacacttt
     1141 gccgctgcgc cggtcgttgc cgttccgggc gcattgttca gcgtgtggca caatatctcc
     1201 ggctcgctgc tggcaactta ttgggcggcc aaagccggta aacataaaaa accctaaacg
     1261 cctttcatca taatgccgtc tgaacggggc aaacgaaacc ggcggtttct gcccgacccc
     1321 ttcagacgcc tttgtttgaa atcggcgggg acgattatcc ggctgctccg attcggctac
     1381 ctgcgttcgc cccgcctttc aagccacctc actccctcac ataaaccact tcgacatcgt
     1441 agtcgtcttc gttccaatcg tcatcgtcgt ccgcgccgaa tttgtcctgc cattcctctt
     1501 cattgctcaa agacgaatcc aaaccggctt caagacggag gacggacaac aaatgatata
     1561 tgtcgtccgg aatcggtgcc tcaaacgaaa cggtttcgcc gctttccgga tgggtgaaac
     1621 tcaagcggta ggcgtgcaat gcctgacgcg cgcccaaact tttaacggct tctttcaccg
     1681 ggtcgctgca cggatggcgc gggttgccgt acacgggatc gccggcaagc ggatggttgg
     1741 cctcgcgcat atggacgcgg atttggtgcg tcctgcccgt ttcgagcgag cattcgatat
     1801 agctgtgggt aagatagcgt tccaacactt tgacgtgggt aacggctggt ttgccgccga
     1861 atttgacgac tgccattttc aggcggttgt gcggatcgcg tccgatttgg gtttcgattt
     1921 taccgtcgaa ggggacgatg ccgttggcga cggcgcggta gatgcgtttg accgtgcgct
     1981 cttgaagctg cctcacgagg gaattttgcg ccggcagggt tttggcaacc accatcagcc
     2041 cgctggtttc tttgtccaaa cggtgtacga tgcctgcacg cggtacttgg ctcaattcgg
     2101 gacagtgcgc caacaggccg ttgagcagcg tccccctcca gttgcctgcc gccggatgca
     2161 ccaccagtcc ggccggtttg ttgacgacaa tgacggtatc gtcttcgtaa acaatatcca
     2221 aagccatagg ctctggaaca aacgccagat tttcctcact cggacggacg gttacacgga
     2281 tttgctcacc gcctatcatt ttgtctttgg gttgcgaagg tttatcgttt acaataaccg
     2341 cgccctcttt aatccataat gtcaggcggc tgcgcgaata gtcgggcaga agtttcgcca
     2401 acaccgcatc caaccgcccg ccggcaagct cgagcggaac ggtcagaccg atacggtttt
     2461 cagtttcgga agctgacgca aagtctgaat cgtcgctata atcggattca ttatcaaagg
     2521 aagtattctg catgaaaaaa attcttttaa cggtttcatt aggtttggca ctgagtgcct
     2581 gtgccactca aggtacggtc gataaagatg cccagattac ccaagattgg agtgtggaga
     2641 agctctatgc cgaagcccag gacgaattga acagcagcaa ttatacgcgg gctgtcaagt
     2701 tatacgaaat cttggaatcg cgcttcccca ccagccgcca tgcccggcag tcgcaactgg
     2761 ataccgcata cgcctattat aaagacgatg aaaaagacaa agctctggcg gcaatcgaac
     2821 gcttccgccg ccttcacccg caacacccga atatggatta cgcgctatac ctgcgcggtt
     2881 tggtactgtt caacgaagat cagtctttct tgaacaagct ggcctcgcaa gactggtccg
     2941 accgcgaccc gaaagccaac cgcgaagcgt accaggcgtt tgcggaactc gtccaacgct
     3001 tccccaacag caaatacgcc gccgatgcga ccgcacgtat ggtcaaactg gtcgatgcac
     3061 tgggcggcaa tgaaatgtcg gtggcgcgct actacatgaa acgcggtgca tatatcgccg
     3121 ccgccaaccg cgcccaaaaa atcatcggca gctaccagaa tacgcgttat gtcgaagaat
     3181 cgctcgccat attggaactt gcctaccaaa aactcggcaa accacagctt gccgccgata
     3241 cgcgccgcgt gttggaaacc aacttcccga aaagcccgtt tttgacgcac gcttggcagc
     3301 ccgacgatat gccctggtgg cgttactggc attaagccca tcttcgtaat gccgtctgaa
     3361 gccgtttcag acggcatttt atgtttcaaa cctcattcaa acggtttttt ctgccaatag
     3421 aatttatacc ctctcaaacg ttgagccttc acgccgccgc gccccaagcc gaattgcagc
     3481 gcgcccgaca aatcggtacg caacaatttg atgccgtgtg cacggatacg gttttgcacc
     3541 gcttcggtcg gatgtttgta ggcgttggca taaccgcttg aagcaacggc atattcgggc
     3601 gagacggcat tgaggaacac gcccgacgag gacgtattgc tgccgtgatg tcccaaaacc
     3661 aaaacctggc tgtacaggtt gcctccatac ttgccgacca ggctttcctc gcccttcgta
     3721 tccaaatcgc ccgttaccag cagtgccgca ccgcccgcca caacacgcaa aacacaactt
     3781 ttcccattat catcgatgtt tttgcgttca gacggcatca aaaactcaaa atccacgccg
     3841 tcccattgcc aacgctgttc cgcacaatgc cgcgccccct cataaaattc cggctgcccg
     3901 gcataaatcc cgccgttcgg tatcttgccg actgcctgaa aaccgccgtc gtggtcgctg
     3961 tcgtgatgcg acagaaccag cttgtccaaa cggcggacac ccgccgcatt caaactcggc
     4021 acaatcccca tctgcgccgc cgctgccgtt ccggtgtcaa acaaaagatg atgatttgcc
     4081 gtctgaaccg acaccgacaa accctgcccc gcatcccaaa ccgtaaccgc aacctcattt
     4141 tccggcacgc cgggtgaacg gtaaaacaca aaccctgcca acagcaacac cgcccacgga
     4201 cgcaaaccca agccgcgcgg cagcagcaac agcaaagcgg cacacaccgc caacaccaac
     4261 agcggcaaag gtgcggcggc aacggcaaac tcgggcgaca catcggcaag ccacaccaaa
     4321 aaccgcaaag tatattccgc caaaaatgcc cccaactgtt gcaaaggcgc aaacggcacg
     4381 accgaaccca gcaacgccag cggcgtcaat acccaagaaa accacggaat cgccaccgca
     4441 ttgaccaaag ggctgattaa aggcagcgaa gcaaacagat aaccgagcaa aaccagcgac
     4501 aacaccgaag ccgcccactg cccgcgcacg gcggtttgcc gtttcccttc gtgcaaacgc
     4561 cccgaacacg cccatatcag ggccgccacc aaaccgaaag acagccaagt ccccacaccc
     4621 aagaccgccg acgggtcgaa cagcagcacc gccgccaacg cctgccacca cgtcgcccac
     4681 gccgacaatc ttcccctgcg ccaagcccac gcaaacgccg ccaacatcaa aacgctgcgc
     4741 tgcgtcggca cggaaaaacc ggcaagcagc gcgtaaaaca gcgcgcctgc acaccccgcc
     4801 gccaaaaccc acgcgcgcgg ccgggcagga aggcgcgggg aacaggcaag cagccgcttc
     4861 gccagccacg caaacattac cgccaccatc gtaacgtgca aacccgaaat actgaccaaa
     4921 tgcgtcagcc ccaacggccg gaacgcctgc cacaattcgg ggcgcaatgc cgactgttcg
     4981 cccacgctca acgcgcgcat cagcccgatg ccgtctgaaa gcccgccgtc cgcatccgcc
     5041 tgctgccaat tacggctgat gcggctgcgc caaaccgcaa tcccccaacc gcttccgcca
     5101 tgcagcaaaa ccctgtccgc accgaccgtc cccgcgccgc ctatcccgtt ggataatgcc
     5161 cacgcctcac ggttcaaacc gcgcaggttc aattcgccga cgacggggtg cacacgtgcc
     5221 gttatccgcc atctgctccc gaccgcccat tcgcgccgtt tgtagtccga cagcagcaaa
     5281 tcaaacgtcc gaccaccgct gtccacagcc tttgccgcaa actgcacgcg ccgcccgtcc
     5341 gacctcggca tatccgccac ttccaccgtc aacggcacac ctgaaaccgc ctccgcccgc
     5401 cattgcgaag acagtgccgc ttccgttctg aatacgccgt aagccgcgcc cgccaacacg
     5461 cacagcatca gaccggcaaa cgcaaaacgc cttgcaaaca cagcaaaaac cgcaaaagcc
     5521 gccagccaaa acagccaatg cggcacgacc ggcagcgcaa acgaagccgc cgcgccgaca
     5581 acccaacacg gcaaaaacca acgacggaac acgtctcccc accccagata tttcaaaaat
     5641 aaacggataa taaagcaaat ccgaaaaacc gtcataacaa gatttgaaaa tgatatagtg
     5701 gtttaaattt aaatcaggac aaggcgacga agccgcagac agtacaagca gtacgacaag
     5761 gcgaggcaac gctgtactgg tttttgttaa accactataa gaatgcaaac gaaaagcctc
     5821 agtattatcc gagacaacgg caaactttcc atcgtcattc ccgcacaagc gggaatccaa
     5881 acttgtccgc acggaaactt atcggataaa acggttt
//
LOCUS       DAWKST010000125         5746 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.125, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000125 DAWKST010000000
VERSION     DAWKST010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.125"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(238..2370)
                     /locus_tag="WJL87_001597"
     CDS             complement(238..2370)
                     /locus_tag="WJL87_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEZ7800008.1"
                     /translation="MSIVSAPLPALSALIILAHYHGIAANPADIQHEFCTSAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPIKRLAMATLPALVWCDDGNHFILAKTDGEGEHAQFLIQ
                     DLVTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
     gene            complement(3170..3823)
                     /locus_tag="WJL87_001598"
     CDS             complement(3170..3823)
                     /locus_tag="WJL87_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ7800009.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAVLFY
                     RKIRMVISHYPALAADEVFEGSVELDESYFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVDNARSETLLPVIKRKIMPDSIVYTDSLSSYDKLHVSGFIHHRINHSKEFADRQ
                     NHINGIENFWNQAKRALRKYNEIDRKSFPLFLKECKFRFNFGTPSRQLKILRDWCGI"
     gene            4160..4594
                     /locus_tag="WJL87_001599"
     CDS             4160..4594
                     /locus_tag="WJL87_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800010.1"
                     /translation="MDNKKEFINNLTNRYMWIYPLVLNILFLPFYQSYQSFFIALGCL
                     FALVRKMQSLDFKLQNHIVLLNIKSAWADKKVFLIRIVVSWLAVMEIWMCFISESSTW
                     VCGAFCLNSEILEKIFRGFGYSGSLYFLFILMIDLNKLRESI"
     gene            4870..5589
                     /locus_tag="WJL87_001600"
     CDS             4870..5589
                     /locus_tag="WJL87_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800011.1"
                     /translation="MKNKNYLLVFIVLHIALIVINIVFGYFVFLFDFFAFLFFANVFL
                     AVNLLFLEKNIKNKLLFLLPISIIIWMVIHISMINIKFYKFEHQIKEQNISSITGVIK
                     PHDSYNYVYDSNGYAKLKDNHRYGRVIRETPYIDVVASDVKNKSIRLSLVCGIHSYAP
                     CANFIKFAKKPVKIYFYNQPQGDFIDNVIFEINDGNKSLYLLDKYKTFFLIENSVCIV
                     LIILYLKFNLLLYRTYFNELE"
BASE COUNT         1757 a         1231 c         1180 g         1578 t
ORIGIN      
        1 aagaaggtgc tcatgagaaa tcccctaaat gtcttggtgg gaatttaggg gattttgggg
       61 aattttgcaa aggtctctag atgagtgaaa aagaagtgca ggctgcctaa aaagacagaa
      121 aaagtctttc cggcagcctg cactttggtt tcatttcagt cagtaaaccc agtaaacgac
      181 ggtctgaaaa cgcagaacgt tacgaaaaaa gcagcctaca cgcccatccc ccgccttcta
      241 cccgttctgt aaatcataca gatagcggta atatccgttc ggcttcgcca gcaattcctg
      301 ctgtgttccc gcttccacaa tcctgccttt atccatggca atgatccggt gtgccgtttt
      361 aacagtggac agacggtggg cgataatcag caccgtccgg ttggcgcaaa tggcctgcat
      421 gttctgcata atcgctcgtt cactttcata atccagcgcg ctggtggctt catcaaaaat
      481 cagaatgcgc ggattggtga ttaacgcgcg ggcaatcgca atacgctgcc gctgtccgcc
      541 cgacaagccg gccccttgtt cgcccaccac ggtgccgtag ccttccggca gctccataat
      601 aaactcgtgt gcgcccgcca gtttggctgc ttcgataatg cgttccagcg gcatacccgt
      661 atccgtcagc gcgatattgt cgcgtatgct gcggttgagc agcacattct cctgcaagac
      721 cacgccgacc tgccgccgca gccaggcagg agcggccaaa gccaaatcgt tgccgtccac
      781 caacacccgt ccctgctccg gtacatacag acgctgcacc aatttggtga gtgtggattt
      841 gcccgacccc gaacgtccca caatccccag cacttccccc gcccgaatcc gcaggttcaa
      901 atcctgcaaa atcagcctgc cgtccgcctt atagcggaaa tcgacatgtt cgaacgtaat
      961 ctccccccgg atatcgggca aagccaaatg cgaagacgca ttctcggtcg gcgcattcag
     1021 aatatccccc aaacgcgcca ccgaaatccc cacctgctgg aaatcctgcc acaactgcgc
     1081 caaacggata acaggcgccg ccacctgtcc cgagagcata ttaaacgcaa tcagctgccc
     1141 caccgtcagc ttgccctcaa ttaccagccg tgcgccaatc cacaacgtcg ccaccgtcac
     1201 cagcttctga atcagctgca ccccctgctg gccgaccacc gccaacttcg ttacccgaaa
     1261 tcccgaagcc acataagccg ccaactgatt gtcccaacgc tgcgtcatct gcggctccac
     1321 cgccatcgcc tttaccgtac ccaccgcagt gatgctttct actaaaaacg actggttgtc
     1381 tgcattgcgc gcgaacttat cgttcagacg cgtccgcagt atcggactga taaatgccga
     1441 ccaaaacgca taggcaggca acgaagccaa taccacccaa gtcagagtgg agctgtaata
     1501 ccacatcacc gccagaaaga taaacgaaaa cgccaaatcc aacaccgaag tcagcgcctg
     1561 accggtcaag aaattgcgaa tctgctccaa ttcccgcacc cgagccaccg tatcacccac
     1621 tcgtctgtgc tcgaaatagg ataaaggcag ggaaagcaga tgccggaaca aacgcgcgcc
     1681 caattccaca tcaatacgtg aagtcgtatg tgcaaacaga tacgtccgca aaccgcccaa
     1741 cacaatctca aacagcgaca ccaccaacaa agccaccgac accacatcca aagtagagaa
     1801 tccccgatgt accagcacct tgtccatcac cacttggaaa aacagaggcg taatcagcgc
     1861 aaacagctgc aacaccaccg acaccaccaa tacttcaaaa aacaaccggc ggtatttgat
     1921 taccgccgga ataaaccagg taaagtcaaa ctttgccaaa ctgcccaata ccgaagcgcg
     1981 ggaagcaacc aatatcagtt tgcccgaata tctgttagaa aattcggcaa aagacaatac
     2041 cgcagactta ttcgtaacca aatcctgtat caaaaattgg gcatgctcac cctcaccgtc
     2101 tgttttggcc aaaatgaaat ggttgccgtc atcacaccat accaatgcgg gtaaagtcgc
     2161 catagccaaa cgtttaatag gctggcggac tacctttgcc ttcaatccca aagatttggc
     2221 ggctaacagc cattgcgttt catttaaatc gctctgtgcg gaagtacaaa attcatgctg
     2281 tatatcggca ggattggcgg caatgccgtg gtaatgggcg aggatgatga gggcggaaag
     2341 ggcggggagc ggtgcggata cgatagacat aaataaaata tagttagatt ggatgtggat
     2401 aacggctggc tggaaaagga atatattaag tagaaagaaa tatataaata aaacagcaga
     2461 acgcattgta aggatatata tgggaattgt aaagagaaag tatggaaaag ttctcgtttc
     2521 aggaaggtaa aacggcttag gaatcgagtt agatgaggat gcctcgcacc tctcgtgcct
     2581 cctgcatacc gttaaggcac agggttaagg tgcaggctgc tccgaactct gttgcggtcg
     2641 ggtaatgtta ttttttgtgt ttcaggcagc ctgaaatatc tgtatatttt tgttttaaat
     2701 agattttaaa gattgataac tgttcttgac gatttttcaa gaaaggagta aatttcaaga
     2761 aaggagtaaa gtgacttatt atcaatgaca agcaacgcgc gaagtgacaa ggaaaactat
     2821 ctacttaaat tctaaggagg cttcgaatat cataaaccaa tcagaaacat agagataaaa
     2881 attatgtaca aatataatcc tcttatacaa tttattgcac agttgattat gtcttatgga
     2941 gcaagcgtag ggtgggcact tgctgcccac gcgtttcata tttcaaggca gcctgaaact
     3001 gtgtgggcat aatgcctacc ctacatccca aaaaaacaag cgcagcctgc gtgtgtaggg
     3061 tgcgaacttt cggcaggcag acacgcggtt ttatagtttc aagctgagtt gggatgctta
     3121 agataaagta caaaacctta aaaaataagg gggctgtact agattagccc taaattccac
     3181 accaatcccg caaaattttt agctgtcggg acggtgtgcc gaagttaaac cgaaatttgc
     3241 attctttcaa gaacagcggg aaagatttac gatcaatttc gttgtatttg cgcaaggcgc
     3301 gtttcgcctg attccaaaag ttttcaatgc cgttaatgtg gttctgacgg tctgcaaact
     3361 ccttagaatg gttgatgcgg tgatggataa aaccgcttac gtgcagcttg tcatagctgc
     3421 tcagactatc ggtataaaca atactgtccg gcatgatttt ccttttgatg acagggagta
     3481 acgtttcaga cctggcatta tctacaacaa cggtatagac ccgtccgttg cgtttcagaa
     3541 tgccgaagac aaccactttt cctgccgcac cgcgaccgcg tctgccttta cgccgtccgc
     3601 cgaaatagct ttcgtccaac tcgacagagc cctcaaaaac ctcatcggca gccaaggccg
     3661 gataatggct gatgaccata cggattttgc ggtagaacag gactgccgaa ttgggatgga
     3721 tacccaaaat atcggcagca gaacgggcgg taacttcgag tacaaaaaaa cggagcagct
     3781 ctttctgtac ttttttcttt aatttgcagt gtgttatctt catatttcgg gggtaacata
     3841 tctgctaatc tagtacagcc tcaaacaaaa aagagaaatt ttaatttcgc taaatcgcat
     3901 aaagattaat caagagtatc attaaatgat atgagtgagc atctataatg ccaagaagag
     3961 ttgttaagac ataacgatta ttgaaataga ttgtaaaata gatacttaga tagtctgaaa
     4021 aacggatttg tgaaactttt tattacgcgc catcatttga aaatgaaact taaaaaacac
     4081 ttatcataat aaatattttc tttacgttgt ttgctaataa actcagtgca atatcagcgc
     4141 aatattttat ggaaatttta tggataacaa aaaagaattt attaataatt taacaaatag
     4201 gtatatgtgg atctatccat tggtcttaaa tattctattt ctaccttttt accagtccta
     4261 ccaatctttt tttattgcgc ttggttgttt gtttgcactg gttagaaaaa tgcaaagctt
     4321 agattttaaa ttacaaaatc atattgtatt gttaaatata aaaagtgctt gggcagataa
     4381 aaaagtattt ttgattagga tagtagtgtc atggttggca gtaatggaaa tatggatgtg
     4441 ttttatttcg gaatcatcaa cgtgggtatg cggtgctttt tgtttaaata gtgaaatatt
     4501 ggaaaaaatt tttcgtggct ttggttattc tggtagttta tattttttat ttatattgat
     4561 gattgatctc aataagttaa gagagagtat ttgaatttca ttttttgttt gacttaaact
     4621 caaggagagt aacaatgatt ggtagtggtg atactaaaca atgcaaaaaa ttttctgcgt
     4681 gtgatggaaa ataccacgtc tacgatcccc tcgccctaga cttggacggc gacggcatag
     4741 aaacagtcac cgccaaaggc ttttcaggca gcctgaagac tgagagagtg aatacgatga
     4801 gtatacactc tatgccacta aattgatatt cactaaatca taccagctat attttattta
     4861 atgagacata tgaaaaataa aaattattta ctagtattta tagttttaca tatagccttg
     4921 atagtaatta atatagtgtt tggttatttt gtttttctat ttgatttttt tgcgtttttg
     4981 ttttttgcaa acgtctttct tgctgtaaat ttattatttt tagaaaaaaa cataaaaaac
     5041 aaattattgt ttttattgcc gatttctatt attatatgga tggtaattca tattagtatg
     5101 ataaatataa aattttataa atttgagcat caaataaagg aacaaaatat atcctcgatt
     5161 actggggtga taaaaccaca tgatagttat aattatgttt atgactcaaa tggatatgct
     5221 aaattaaaag ataatcatag atatggtagg gtaattagag aaacacctta tattgatgta
     5281 gttgcatctg atgttaaaaa taaatccata agattaagct tggtttgtgg tattcattca
     5341 tatgctccat gtgccaattt tataaaattt gcaaaaaaac ctgttaaaat ttatttttat
     5401 aatcaacctc aaggagattt tatagataat gtaatatttg aaattaatga tggaaacaaa
     5461 agtttgtact tgttagataa gtataaaaca ttttttctta ttgaaaacag tgtttgtatc
     5521 gtattaatta ttttatattt aaaatttaat ttgcttttat ataggactta cttcaatgag
     5581 ttggaatagt tttggtaatt ttatgagcgc acgctcatcc gcgttagcag aatttggaaa
     5641 tatggttgct aatttagttt ctgcaaaaaa tgagaaagat atctcgaaac gtaatgaata
     5701 ttacaaacaa gctggttata gtgcattatt agcatttggt aatttg
//
LOCUS       DAWKST010000126         5720 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.126, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000126 DAWKST010000000
VERSION     DAWKST010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.126"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            34..1389
                     /locus_tag="WJL87_001601"
     CDS             34..1389
                     /locus_tag="WJL87_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ7800012.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHNLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLFYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKSY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLIITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWFELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMMGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1393..2421
                     /locus_tag="WJL87_001602"
     CDS             1393..2421
                     /locus_tag="WJL87_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ7800013.1"
                     /translation="MSDTPATPATPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGG
                     IVQEFSVLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRV
                     EANEGRNALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDE
                     DNRLTVSYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVS
                     DDLEVFDFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIY
                     SDRNGLVIFTAAPQDFARHDAGVYDALATEAQTLPDSLNWPEFGNIRLNKGDTREATI
                     AYGIESLS"
     gene            2655..4913
                     /locus_tag="WJL87_001603"
     CDS             2655..4913
                     /locus_tag="WJL87_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ7800014.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFDVCKFLSVAQKELALIRWEAVSIDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDDRSYIATQTVTNPFGVEQFIVNAEQ
                     TFAGSFKALGGSQTDWQVSNSFEAEVGGTPKTFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDEGYLAKEGLE
                     VLVEVSRFWADRVHFSKRNGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYRRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPSAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDI
                     TLNGSHTAALEK"
     gene            4926..5591
                     /gene="pgmB"
                     /locus_tag="WJL87_001604"
     CDS             4926..5591
                     /gene="pgmB"
                     /locus_tag="WJL87_001604"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ7800015.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGISIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVSEAEFAELTRRKNDNYVEMIQAVKPEDVYPGILPLLET
                     LKANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRRCIGIEDAAAGVAAIKASGALPIGVGKAEDLGSDIPLVSGTAELTYAYLQNVWAQ
                     SGR"
BASE COUNT         1288 a         1657 c         1551 g         1224 t
ORIGIN      
        1 ctatactttt caaatcaaaa aaggatttac cttatgtcgg aatatacgcc tcaaacagca
       61 aaacaaggtt tgcccgcgct ggcaaaaagc acgatttgga tgctcagttt cggctttctc
      121 ggcgttcaga cggcctttac cctgcaaagc tcgcaaatga gccgcatttt tcaaacgcta
      181 ggcgcagacc cgcacaattt gggctggttt ttcatcctgc cgccgctggc ggggatgctg
      241 gtgcagccga ttgtcggcca ttactccgac cgcacttgga agccgcgttt aggcggccgc
      301 cgtctgccgt atctgtttta tggcacgctg attgcggtta ttgtgatgat tttgatgccg
      361 aactcgggca gcttcggttt cggctatgcg tcgctggcgg ctttgtcgtt cggcgcgctg
      421 atgattgcgc tgttggacgt gtcgtcgaat atggcgatgc agccgtttaa gatgatggtc
      481 ggcgatatgg tcaacgagga gcagaaaagc tacgcctacg ggattcaaag tttcttagcg
      541 aatacgggcg cggtcgtggc ggcgattctg ccgtttgtgt tcgcgtatat cggtttggcg
      601 aacaccgccg agaaaggcgt tgtgccacaa accgtggtcg tagcattcta tgtgggcgcg
      661 gcattactga ttattaccag tgcgttcaca atcttcaaag tcaaagaata cgacccggaa
      721 acctatgccc gttaccacgg catcgatgtc gccgcgaatc aggaaaaagc caactggttc
      781 gaacttttaa aaacggcacc taaagcgttt tggacggtta ctctggtaca gtttttctgc
      841 tggttcgcct tccaatatat gtggacttac tcggcaggcg cgattgcgga aaacgtctgg
      901 cacaccactg atgcgtcttc cgtaggttat caggaggcgg gcaactggta cggcgttttg
      961 gcggcggtgc agtcggttgc ggcggtgatt tgttcgtttg tattggcgaa agtgccgaat
     1021 aaataccata aggcgggtta tttcggctgt ttggctttgg gcgcgctcgg ctttttctcc
     1081 gttttcttca tcggcaacca atacgcgctg gtgttgtctt ataccttaat cggcatcgct
     1141 tgggcgggca ttatcactta tccgctgacg attgtgacca acgccttgtc gggcaagcat
     1201 atgggcactt acttgggcct gtttaacggc tctatctgta tgccgcaaat cgtcgcttcg
     1261 ctgttgagtt tcgtgctttt cccgatgatg ggcggcttgc aggccactat gttcttggta
     1321 gggggcgtcg tcctgctgct gggcgcgttt tccgtgttcc tgattaaaga aacacacggc
     1381 ggggtttgag cgatgagcga tacccccgct acccccgcta cccccgctac ccgcgatttc
     1441 ggcctgatcg acgggcgtgc cgtaaccggc tatgtgctgt ccaatcggcg tggtacgcgt
     1501 gtctgcgtgc tggacttggg cgggattgtg caggaatttt ccgttttggc agacggcgtg
     1561 cgcgaaaacc tcgtggtgtc gttcgacgat gcggcttcct atgcggacaa tccgtttcag
     1621 attaacaagc agatagggcg cgtggccgga cgcatccgcg gtgcggcgtt cgacatcaac
     1681 ggcaggactt accgcgtgga ggccaacgaa ggcaggaacg cgctgcacgg cggttcgcac
     1741 gggctggccg ttacccgttt caacgcggtg gcggcagacg gccgttcggt ggtgctgcgc
     1801 agccgcctgc aacagtcggc cgacggttat cccaacgatt tggatttgga tatttcctac
     1861 cgcttggacg aggacaaccg gcttaccgtt agctatcgcg ccaccgcgct cggcgacacg
     1921 gtgttcgacc cgacgctgca catttactgg cggctggacg cgggcctgca cgatgcggtt
     1981 ctgcatattc cgcagggcgg acatattccg gccgatgccg aaaaactgcc cgtctcaacg
     2041 gtttcagacg acctcgaagt atttgatttc agccggccca agccgctgga tgccgccgtt
     2101 gccgccctgc gccgcgaaac gggtcgggcc ggttttgacg acgcttaccg cgtgccgtcc
     2161 gatataggcc gtcccgccgc tgtgttgcaa gccggacgcc gccgtcgtat cagcatatac
     2221 agcgaccgca acggcttggt catctttacc gccgccccgc aggatttcgc gcggcacgat
     2281 gcgggcgttt acgacgcgct ggcgaccgag gcgcagacgc tgcccgacag cctgaattgg
     2341 cccgagttcg gcaatattcg tctgaacaag ggtgatacca gggaggcgac gattgcttac
     2401 ggcatcgaat ccctttctta ggagcttcct aacaccggtt gcagacggcc tttttatagt
     2461 ggattaacaa aaaccggtac ggcgttgcct cggcttagct caaagagaac gattctctaa
     2521 ggtgctgaag caccaagtga atcggttccg tactatttgt actgtttgcg gcttcgtcgc
     2581 cttgtcctga tttttgttaa tccactataa gatttcacca ttccctcaaa tcaatccaaa
     2641 caggagcttc ataaatgtac acaagaatta tggaaatcag cccttggacg ctgcgttcgg
     2701 caaagctgga aaaagaacac aaacggctgc aagagagcct gaccagtttg ggcaacggct
     2761 atatgggtat gcgcggcagc tttgaggaaa cctactccgc cgacagccac ttgggcacct
     2821 acatcgccgg cgtgtggttc cccgacaaaa cccgcgtcgg ctggtggaaa aacggctatc
     2881 ccaaatattt cggcaaagcc atcaacgcgc tcaatttcag caaagtcaaa atctttgtcg
     2941 atggtcaaga ggtggacttg gcgaaaaacg atgttgccgg cttctccgtc gaactcgata
     3001 tgcagcacgg cgtgttgcgc cgctcgttca ccgtattcgg cgtgcgtttc gatgtgtgca
     3061 aattcctgtc cgtcgcacaa aaagagctgg ctctcatccg ctgggaagcc gtatctatcg
     3121 acggcaaaac ccaccaagtc cgcatcgatt ccatcatcga tgccgacgtg aaaaacgaag
     3181 attccaacta cgaagaaaaa ttctggcaag tattggacaa aggcgtttca gacgaccgct
     3241 cctacattgc cacccaaacc gtcaccaatc ccttcggcgt ggaacaattt atcgtcaacg
     3301 ccgagcaaac tttcgccggc agcttcaaag ccctcggcgg cagccaaacc gactggcagg
     3361 tctccaattc tttcgaggcc gaagtcggcg gcacgcccaa aacctttgaa aaacgggtta
     3421 ttgttaccac cagccgcgat tatcagggct tggaagcagt gaaagccgca ggccgtgcct
     3481 tgtcggaaaa agtcgcaggc gttgcgtttg aaaccttgct ggacgcgcac aaagcaggct
     3541 ggctgcaccg ttgggaaatc gccgacgtgg tcatcgaagg cagcgacgaa gcgcagcaag
     3601 gcatccgttt caatctgttc caactgttct ccacctacta cggcgaagat gcgcgcctga
     3661 acatcggccc caaaggcttt accggcgaaa aatacggcgg cgcgacctat tgggataccg
     3721 aagcctatgc cgtaccgctc tacctcgcac tggcggaacc cgaagttacc cgcaacctgc
     3781 tgcaataccg ccgcaaccaa ctgccgcagg cgcagcacaa cgcgcgcgaa cagggcttgg
     3841 cgggcgcact ctatccgatg gtaacgttta cgggtatcga gtgccacaac gaatgggaaa
     3901 tcaccttcga ggaaatccac cgcaacggcg cgattcctta cgccatctac aactacacca
     3961 actacaccgg cgacgagggc tatcttgcca aagaaggctt ggaagtcttg gtcgaagtgt
     4021 cccgcttctg ggcggaccgc gtccacttct ccaaacgcaa cggcaaatac atgattcacg
     4081 gcgtaaccgg cccgaacgaa tacgaaaaca acatcaacaa caactggtac accaacaccc
     4141 tcgccgcatg ggtattggac tacacccgcg aagccttggc gaaatacccg cgtccggatt
     4201 tgaacgtgcg tgccgccgag ttggaaaaat gggcggacat cagcgcgaat atgtaccgtc
     4261 cgcatgacga agaactcggc gtattcgtgc aacacgacgg cttcctcgac aaagacatcc
     4321 gccccgtgtc cgcgctttcg cccgacgatt tgccgctcaa ccagaaatgg tcgtgggaca
     4381 aaatcctgcg ttcgcccttt atcaaacagg cggacgtatt gcaaggcatc tatttcttcg
     4441 gcgaccgttt caatatcgac gaaaaacgcc gcaatttcga cttctacgaa ccgatgaccg
     4501 tgcacgaaag ctcgttgtcg ccgtgtatcc actctattct cgccgccgaa ctgggcaaag
     4561 aagaaaaagc cgtggaaatg taccggcgca ccgcacgcct ggacttggac aactacaaca
     4621 acgacaccga agacggcctg cacattactt ctatgactgg ctcgtggctc gccatcgtcc
     4681 aaggtttcgc ccaaatgaaa acttggggcg gtaaactcag cttcgcaccg ttcctgccga
     4741 gcgcatggac aggctacgcc ttccatatca actaccgagg ccgtctgatt aaagtcgccg
     4801 tcggcaaaga aaacgtcgtc ttcaccctgc tcaaaggcga gccgctcgat ttgcaggtgt
     4861 acggcaaaga catcacactc aacggcagcc ataccgccgc gttggaaaaa taaggagggc
     4921 gcaaaatgac gtttaccgca gtgctcttcg atctcgacgg cgtcatcacc gacaccgccg
     4981 aataccacta ccgcgcgtgg aaaaaactcg ccgaagaact gggcatcagc atcgaccgca
     5041 agtttaacga gcagctcaaa ggcgtgtcgc gcgacgattc gctcaaacgc atcctcgcgc
     5101 acggcggcaa aaccgtcagc gaagccgagt tcgccgaact gacccgccgt aaaaacgaca
     5161 actacgtcga gatgattcag gcagtcaaac ccgaagacgt gtatcccggc attttgcccc
     5221 tgctggaaac attgaaagca aacggcaaaa aaatcgccct cgcgtccgcc agtaaaaacg
     5281 gcccgttcct gctcgaacgc atggggctga cccacttctt cgacaccgtc gccgaccctg
     5341 ccgccgtcgc gcattccaaa cccgcccccg acatcttcct cgccgccgcc gagggcgtgg
     5401 gcgcggacat ccgccgctgc atcggcattg aagacgctgc cgcaggtgtc gccgccatca
     5461 aagcctccgg cgccttaccc atcggtgtgg gcaaagccga agacttgggt agcgacatcc
     5521 cgctggtgtc cggcaccgcc gagctgacct acgcctacct gcaaaacgtg tgggcacagt
     5581 cgggcaggta aaacgcgtca gataaagtgt taaggaagta aaaggccgtc tgaacagtgt
     5641 ttcagacggc ctgagacctt tgcaaaattc ctgtccctcc cgacagccga aacccaaaca
     5701 caggatttcg gctgttttcg
//
LOCUS       DAWKST010000127         5694 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.127, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000127 DAWKST010000000
VERSION     DAWKST010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.127"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(81..842)
                     /locus_tag="WJL87_001605"
     CDS             complement(81..842)
                     /locus_tag="WJL87_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800016.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKVVPTYAPLSRPSETGTDASVP
                     KLDDASRNRVLKAYLKKWQEEHNAAVADCGEEIETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRIRFDTKKDSVEFETGQIEGGENADEAESGTNEPAVSENGENDAEETLMPV
                     QLNILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            942..1193
                     /locus_tag="WJL87_001606"
     CDS             942..1193
                     /locus_tag="WJL87_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800017.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPADTAEVA
                     LESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            1245..2051
                     /locus_tag="WJL87_001607"
     CDS             1245..2051
                     /locus_tag="WJL87_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ7800018.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDEQAAK"
     gene            2358..3881
                     /locus_tag="WJL87_001608"
     CDS             2358..3881
                     /locus_tag="WJL87_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ7800019.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGGKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKSIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTGLLGMIGKSERGVATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPRKWQAKIGIIPVES"
     gene            3979..5391
                     /gene="trkA"
                     /locus_tag="WJL87_001609"
     CDS             3979..5391
                     /gene="trkA"
                     /locus_tag="WJL87_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ7800020.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
BASE COUNT         1434 a         1716 c         1385 g         1159 t
ORIGIN      
        1 ttttgcgcct gttccatgag gctttcaagt cggatgagaa tgcaaatgcc gtctgaaacg
       61 gctttcagac ggcatggcaa tcagcgtttg tattttaact cgtacttgat gtcgttgagg
      121 attttgcgga catcgtgttc caacacgtct tcgactaccg cccccgcctg ctcgtgcagc
      181 atctgctgga gctgataggt gaaaaccgcc atctgctttt gcaccgccgt tcggatgatg
      241 ccgttgacgg tatcggtcag atgggggcgc aggcgtttga tcagccgttc ggtcagctcc
      301 tgttcggaca ggcagaacac ttcgcgccgg ttgacggctt tcgggttcag gatattgagt
      361 tggacgggca tcaacgtttc ttccgcatcg ttttccccgt tttccgaaac cgccggctca
      421 ttcgtgccgg attctgcctc gtcggcgttt tccccgcctt caatctgtcc ggtttcaaat
      481 tcgacactgt cttttttggt atcaaaccgg attctccgcc gcgattcgat gtgtttttcc
      541 gaaaccgaca tttgcaggga agcctgcgcg ttgagccagt tttcctgaag gacgatcatc
      601 gggtcggttt cgatttcttc cccgcaatcg gcaacggcgg cattgtgctc ctcctgccat
      661 ttcttcagat acgcctttaa cacacggttt cggcttgcat cgtccagttt cggcacagac
      721 gcgtccgttc cggtttcaga agggcgggac agcggcgcgt aagtcggcac taccttcata
      781 cggcgcgtct gacgcaggtt ttccaaacgt ttttcccaat tcggctcttt attcgcatcc
      841 attttcggct tccggtcctt aatctttgca agcagacaaa cccgcgccca aagcgcggtt
      901 tgatataatg gcgcatttta acagattcgc gaggatacat catgggcagc atcgaacagc
      961 gtttggaata tctggaagag gcgaacgacg tgctgcgtat gcagaaccac gtcctgtcca
     1021 ccgccttcaa agccttaatc cgcgcccttc ccgccgacac cgccgaagtc gcgctcgagt
     1081 ccatccagct tgcttttgag gacgccttgg cagaattgag ctatgaggac agcccgcata
     1141 cggatttgtt ccacgacgtt acttatgcgt ttttccgtga aaaagaacgt taattttatg
     1201 ttaaactgat tttttaggct ttttgattac cgaaaggaat tttgatgaat atgaaaaaat
     1261 ggattgccgc cgcccttgcc tgttccgcac tcgcgctgtc tgcctgcggc ggtcagggca
     1321 aagatgccgc cgcgcccgcc gcaaaccccg acaaagtgta ccgcgtggct tccaacgccg
     1381 agtttgcccc ctttgaatct ttagactcga aaggcaatgt cgaaggtttc gatgtggatt
     1441 tgatgaacgc gatggcgaag gcgggcaatt ttaaaatcga attcaaacac cagccgtggg
     1501 acagcctttt ccccgccttg aacaacggcg atgcggacgt tgtgatgtcg ggcgtaacca
     1561 ttaccgacga ccgcaaacag tctatggact tcagcgaccc gtattttgaa atcacccaag
     1621 tcgtcctcgt tccgaaaggc aaaaaaatat cttcttccga cgatttgaaa aacatgaaca
     1681 aagtcggcgt ggtaaccggc tacacgggcg atttctccgt atccaaactc ttgggcaacg
     1741 acaatccgaa aatcgcgcgc tttgaaaacg ttcccctgat tatcaaagaa ctggaaaacg
     1801 gcggcttgga ttccgtggtc agcgacagcg cggtcatcgc caattatgtg aaaaacaatc
     1861 cgaccaaagg gatggacttc gttaccctgc ccgacttcac caccgaacac tacggcatcg
     1921 cggtacgcaa aggcgacgaa gcaaccgtca aaatgctgaa cgatgcgttg gaaaaagtac
     1981 gcgaaagcgg cgaatacgac aagatttacg ccaaatattt tgcaaaagaa gacgaacagg
     2041 ccgcaaaata agcccgccca tccgaacaca atgccgtctg aagccctttc agacggcatt
     2101 gttcatcaat cggcctacaa tgaactgcct gctgatttct ccctaccgca aagcaacagg
     2161 caaagattac aaatatcaaa atccgagtaa aacagtattt tattaaaaca aattgataat
     2221 caagagatta gaattatgta ttgtctttac cgtacaaacg ctggcactat ttcaacctga
     2281 taaaaaacag ccttcaaaaa ggttgcttaa aacagcagca gacacttacc gccacaacct
     2341 tgaaaaggaa cacaatcatg accgtcatca aacaggaaga ctttatccaa agcatttgcg
     2401 atgccttcca attcatcagc tactaccatc cgaaagacta catcgacgcg ctttataagg
     2461 cgtggcagaa ggaagaaaac cccgccgcca aagacgcgat gacgcagatt ttggtcaaca
     2521 gccgcatgtg tgccgaaaac aaccgcccca tctgccaaga taccggtatc gcgaccgtct
     2581 tcctcaaagt cggtatggat gtgcagtggg atgcggacat gagcgtcgaa gaaatggtta
     2641 acgaaggcgt gcgccgcgcc tacacttggg aaggcaacac cctgcgcgcc tctgtcctcg
     2701 ccgatccggc cggcaaacgt caaaacacca aagacaacac ccccgccgtc atccatatga
     2761 gcatcgtgcc gggcggtaaa gtcgaagtaa cctgcgcggc aaaaggcggc ggctctgaaa
     2821 acaaatccaa actcgccatg ctcaatcctt ccgacaacat cgtcgattgg gtattgaaaa
     2881 gcatcccaac catgggcgca ggctggtgtc ctcccggcat cttgggcatc ggcatcggcg
     2941 gcacgcccga aaaagccgtg ctgatggcaa aagagtccct gatgagccac atcgacattc
     3001 aagagttgca ggaaaaagca gcgtcaggtg cagaattgtc caccaccgaa gccctgcgcc
     3061 tcgaactctt tgaaaaagtc aacgcgctag gcatcggcgc gcaaggcttg ggcggtctga
     3121 ccaccgtgtt ggacgtgaaa atcctcgatt acccgaccca cgccgcctcc aaaccgattg
     3181 ccatgattcc gaactgcgcc gccacccgcc acgtcgaatt tgaattggac ggctcaggcc
     3241 ctgtcgaact cacgccgccg cgcgtcgaag actggcccga tttgacttac agccccgaca
     3301 acggcaaacg cgtcgatgtc gacaagctga ccaaagaaga agtggcaagc tggaaaaccg
     3361 gcgacgtatt gctgttgaac ggcaaaatcc tcaccggccg cgatgccgca cacaaacgcc
     3421 tcgtcgatat gctcaacaaa ggcgaagaat tgcccgtcga tttcaccaac cgcctgattt
     3481 actacgtcgg ccccgtcgat ccggtcggcg atgaagtcgt cggtccggca ggtccgacca
     3541 cagccacccg catggacaaa ttcacccgcc aaatgctcga acaaaccggc ctcttgggca
     3601 tgatcggcaa atccgagcgc ggcgtggcca cctgcgaagc catcgccgac aacaaagccg
     3661 tgtacctcat ggcagtcggc ggcgcggcgt atctcgtggc aaaagccatc aaatcttcca
     3721 aagtcttggc gttccccgaa ttgggcatgg aagccattta cgaatttgaa gtcaaagaca
     3781 tgcccgtaac cgtcgccgta gatagcaaag gcgaatccat ccacgccacc gccccgcgca
     3841 aatggcaggc gaaaatcggc atcatccccg tcgaatcttg aggcgccatg ccgtctgaac
     3901 acaaaatctg ccttcagacg gcatttccgc ccccggttgc ggtacaatcc accatttcat
     3961 cactcggcga cccacaccgt gaaaatcctc attttaggca acggacaggt aggttctacc
     4021 gtcgcacaaa accttgccgc catacccaac aacgacgtaa ccgttatcga catcgacgaa
     4081 aaagcattgc aggaaacagg cagccgcctc gatgtccaaa ccgttttcgg caacggcgca
     4141 tcccccttca cattagaacg cgccggcgcg gaagatgccg acttgctgct cgcgctctcc
     4201 cgcagcgacg aaaccaacat cgtcgcctgc aaagttgccg ccgacctgtt caacatcccc
     4261 ggccgcatcg cgcgcgtccg ttccagcgaa tacctcgaat acctcagccc caagctcgaa
     4321 aacaacgaaa acggcagcct ttccatattc ggcataaccg aaaccatcag ccccgaacag
     4381 ctcgttaccg aacagcttgc cggcctgata gactgcccgg gcgcattgca ggttttacgt
     4441 tttgcagacg accgcgtgcg gatggtcatc atacaggcgc ggcgcggcgg actgcttgtc
     4501 ggacgcagca ttgccgacat cgcccaagat ttgcccgacg gggccgactg ccaaatctgc
     4561 gccgtttacc gcaacaaccg cctcatcgtc cccgcgccgc aaaccgtcat catcgaaggc
     4621 gacgaaatcc tatttgccgc cgccgccgaa aacatcggcg cggtcatacc cgaattgcgc
     4681 cccaaagaaa ccagcacccg ccgcatcatg attgccggcg gcggcaacat cggctaccgt
     4741 ctcgccaagc agctcgaaca cgcatacaac gtcaaaatca tcgaatgccg gccgcgccgt
     4801 gccgaatgga tagccgaaaa cctcgacaac accctcgtcc tgcaaggttc ggcaaccgac
     4861 gaaaccctgc tcgacaacga atacatcgac gaaatcgacg tattctgcgc cctgaccaac
     4921 gacgacgaaa gcaacattat gtccgccctt ttggcgaaaa acctcggcgc gaagcgcgtc
     4981 atcggcatcg tcaaccgctc aagctacgtc gatttgctcg aaggcaacaa aatcgacatc
     5041 gtcgtctccc cccacctcat caccatcggc tcgatactcg cccacatccg gcgcggcgac
     5101 atcgttgccg tccaccccat ccggcgcggc acggcggaag ccatcgaagt cgtcgcacac
     5161 ggcgacaaaa aaacttccgc catcatcggc aggcgcatca gcggcatcaa atggcccgaa
     5221 ggctgccaca ttgccgccgt cgtccgcgcc ggaaccggcg aaaccattat gggacaccat
     5281 accgaaaccg tcatccaaga cggcgaccac atcatctttt tcgtctcgcg ccggcgcatc
     5341 ctgaacgaac tggaaaaact catccaggtc aaaatgggct ttttcggata aaccgcccca
     5401 ttccggacat attgccgcca agcggtatgg aagcagaata atggtaggtg ggcttcagac
     5461 ggcatccgcc ctccccgtca ttcccgcgta agcgggaatc cagaccttgg gatagcggca
     5521 atattcaaag gttataaaag acccgtcatt cccgcgcagg cgggaatcca gaccttggga
     5581 tagcggcaat attcaaaggt tagctgaagc tttagagatt ctagattccc gtttccacgg
     5641 gaatgacgaa aggttgcggg aatccaaacc attgggcaac agcaatattc aaag
//
LOCUS       DAWKST010000128         5618 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.128, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000128 DAWKST010000000
VERSION     DAWKST010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.128"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(98..1231)
                     /gene="nrdB"
                     /locus_tag="WJL87_001610"
     CDS             complement(98..1231)
                     /gene="nrdB"
                     /locus_tag="WJL87_001610"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ7800021.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGMHNVGGKPITVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(1445..2425)
                     /locus_tag="WJL87_001611"
     CDS             complement(1445..2425)
                     /locus_tag="WJL87_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEZ7800022.1"
                     /translation="MTAARAGLVRFNYLKVKQAVQSKQIKGFKLWKSYDEQLEILRSR
                     GLQIDDEKKALGYLRTIGYYRLSGYFYSFRQFAPESSKSRLDEFITDTRFEDVKSLYM
                     FDKKLRQLALDALERIEVALRVNIAHTLGRHDPLAYKDSKYSATGFDHAEWLDRYNRL
                     VEREKKTGFVKHHLEHYADLPVWVACEVWDFGTMSMLYKGMLNKDKDSIAKLYRLKDG
                     KHLQTHLHAFNFIRNVSAHHSRLWNRDITFKASLKGLSDAEWKSLPINKPFVYFCLMK
                     RMLDVICPRTEWGNRLLALLDEFPDVANEAVSLRDMGLENNPRDWRLWQG"
     gene            complement(2391..4670)
                     /gene="nrdA"
                     /locus_tag="WJL87_001612"
     CDS             complement(2391..4670)
                     /gene="nrdA"
                     /locus_tag="WJL87_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ7800023.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKEARLGYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRAEIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKDAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPNKFEGSKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQADIQDDGCAGGACKI"
     gene            5199..5510
                     /locus_tag="WJL87_001613"
     CDS             5199..5510
                     /locus_tag="WJL87_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HEZ7800024.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYAEQKIQCRAAYEAFDDFDGGSFR
                     R"
BASE COUNT         1407 a         1364 c         1309 g         1538 t
ORIGIN      
        1 gaacaataac cgatcctacc cgctgttttt cccatcatac aacatacaaa tgccgtctga
       61 aacatccggc ttcagacggc attttgtcaa aaaacgttta caactcaaaa tcgcccaaat
      121 catccgtatt cacttcagaa tcgatctgac cgatcaaata agaggatatt tccacttcct
      181 gcggcgcgac ctgtacgttg tcggacgaca gccacgcatt aatccacgga atcgggtttt
      241 gatttgcgcc ttcaaatccg gccggcaacc ccaccgcctg catacgcaga ttggtaatat
      301 attcgacgta ttgggataag atttctttgt tcaaaccgat catcgaaccg tccttaaaca
      361 aatatgccgc ccattctttt tcctgttccg ccgctttttt gaagagttga aaacattcgt
      421 cctgcaactc ggcggcaatt tctgccattt cagaatcatc aacacccgaa cgcatcagat
      481 taagcatatg ctgcgtgccg gtcaggtgca gggcttcgtc gcgggcaatc agtttgatga
      541 ttttggcgtt gccttccatc aactcgcgct cggcaaaagc aaacgagcag gcgaatgaaa
      601 cgtagaaacg gatggcttcc aacacgttga cgcacatcag gcagagatag agttttttct
      661 tcaacccgcg caaagacaca gtaatgggtt ttccgccgac attatgcatc ccttcgccca
      721 acaggttgta atactgggtg tattcgatta agtcatcgta atagcaggca atgtcttcgg
      781 cgcgggcggt aatgtattcg ttttcgacaa tatcatcaaa cacgaccgac ggatcattca
      841 caatattgcg gatgatgtgg gtatagctgc gcgagtgtat ggtttcgctg aagctccacg
      901 tttcaatcca cgtttccagc tcgggaatcg aaaccaaagg cagcaaggca acattcggac
      961 tgcgcccttg gatggaatcg agcagtgttt ggtatttcag attgctgatg aaaatatgtt
     1021 tttcgtgttc aggcaggttg gcgtagtcga tacggtcgcg cgacacgtcg atttcttccg
     1081 gccgccaaaa aaacgacaat tgtttttcaa tcagtttttc aaatacctcg tatttctgct
     1141 ggtcataacg ggcgacattc acaggctgac cgaaaaacat cggctcattc agcgcgtcgt
     1201 ttttggtttt gggaaaggtg ctgtatgaca tgactaggtg ttcgcaggac atggttttct
     1261 caggtttatt taaatttagg aatttggatt tcagacagcc ttatatgttt taagtaaggt
     1321 catctgaatg tatctgtaca ttgaattctt gccttaaata tcgctgttag ttataatctt
     1381 ccccaacaac tcccccaact ttgccaacct acgcggttgg agcaacaagg ttgggggctt
     1441 tcttttatcc ctgccacaat ctccagtctc gcggattatt ttccaaaccc atatcgcgca
     1501 aacttacagc ttcattcgcg acatcgggaa actcatccag taaagcaaga agacggtttc
     1561 cccattcggt tcgcggacaa ataacatcca acatccgttt catcaggcaa aaatacacaa
     1621 acggtttatt gataggcaat gatttccatt ctgcatcaga caaacctttc aagctggctt
     1681 taaaagtaat atccctattc cataatcgac tgtgatgtgc cgaaacgttg cggataaagt
     1741 taaatgcgtg cagatgtgtt tgcagatgct taccgtcttt caaacgatac aatttggcaa
     1801 tagagtcttt atctttattc aacatccctt tatacagcat agacatcgtg ccgaaatccc
     1861 atacttcgca ggctacccag acaggtaagt cggcataatg ttccaaatga tgtttaacaa
     1921 aaccggtttt cttttcgcgc tcgaccagcc tgttatacct atccagccat tccgcatggt
     1981 cgaaacctgt ggcagaatat ttgctatctt tgtaagccaa tgggtcatgc cgtcctaatg
     2041 tatgggcaat atttacccga agcgctactt caattctttc cagtgcatct aatgccaatt
     2101 gccgcaattt cttatcgaac atataaaggc ttttgacatc ttcaaaacga gtatctgtaa
     2161 taaactcatc caacctgctc ttagaacttt caggagcaaa ttgtcggaaa gagtaaaaat
     2221 aaccgcttaa tcggtaataa ccgatggtgc gtaaatatcc taatgccttt ttctcatcat
     2281 caatctgcaa tcctctactt cttagaatct ccaactgctc atcgtaagat ttccaaagct
     2341 tgaagccttt tatctgcttg ctttgcacag cctgtttcac tttcaaatag ttaaatctta
     2401 caagccccgc ccgcgcagcc gtcatcctga atatcggctt gcgtatcgtc cgcgccgtcg
     2461 cgggtgttgt ggtagtacag ggttttgacg ccgtatttgt aagcggtcaa taagtctttg
     2521 agcatttgtt tcatggaaac tttgctgcct tcgaatttgt tcgggtcgta ggcggtattg
     2581 gcggaaatcg attgatcgac gaatttttgc attacgccga caagtttcaa gtagccttcg
     2641 ttgccgggaa gctgccacag ggtttcgtag gcgtctttca gggtttcaaa ttccggtacg
     2701 acttgtttca aaataccgtc ttttgatgct ttgaccgtta ccaatccgcg cggcggctcg
     2761 ataccgttgg tggcgttggc gatttgcgaa ctggtttcag acggcatgag tgcggttaga
     2821 gtagagttac gcaggccgta tttgacgatt tcggcacgca ggctttccca gtcgtaatgc
     2881 aaaggctcgc cgcagacggc atccaaatct tttttgtagg tgtcgatggg cagtttgcct
     2941 tgcgaataaa cggtttggtt aaagagcgta cacgcaccgt attctttggc aaggtttgcc
     3001 gatgctttga gcaggtaata ctgtatggct tcaaaggtac ggtgggtcag accgagcgcg
     3061 gagccgtcgc tgtagcggac accgtttttc gccagataat aagcatagtt aatcacgccg
     3121 atgccgagcg aacggcggcc catagtagag gtacgcgcgg cttctaccgg atatccctga
     3181 taatctaaaa gtgcatcgag cgcacgaacg gtcaagtcgg caagcccttc caattcgtcc
     3241 aagctgttta atgcgcccaa gttaaaggca gacagtgtac acagggcgat ttcgccgttc
     3301 ggatcgttga tattgtccag cggtttggtc ggcagggcga tttccataca caagttggac
     3361 tgatgaacag gcgcgacgcg cggatcgaac gggctgtgcg tattgcagtg atcgacgttt
     3421 tgaatgtaga tgcgcccggt tccggcacgc tcctgcatca gcgtggaaaa caggtcggca
     3481 gccggaatga tgcgcttgcg gatatcaggg tcttgctcgt atttcgtata gagccgctca
     3541 aattcgtctt ggtcggcaaa aaacgcttcg tacaatcccg gaacctcgtt gggcgaaaac
     3601 agcgtaatgt tgccgccctt aatcaggcgg gtgtacagca ggcggttgat ttgcacgccg
     3661 taatcaagct gacggatacg gttgtcttcc acaccgcggt tgtttttcaa caccagcagg
     3721 ctttcggctt cgatatgcca caaggggtag aacaaggttg ccgcgccgcc gcgcacgccg
     3781 ccttgcgaac aggatttgac cgccgcctga aacattttaa agaagggaat gcagccggta
     3841 tgccgcgctt cgccgccccg gatttcgctg tccaaaccgc ggatacgtcc ggcattgatg
     3901 ccgatgcccg cacgctggga aacgtatttc acaatcgcgc tggtagtggc attgatggaa
     3961 tccaaactat cgtcgcattc aatcagcaca cagcttgaga actggcgcgt aggcgtacgc
     4021 acgccgctca taatcggagt cggcagcgat actttaaatg tagaaacggc atcgtaaaac
     4081 cgtttgacgt aacccaagcg cgcctctttc gggtatttgc tgaaaaggca catcgccacc
     4141 aaaacatata aaaactgcgg cgtttcgtaa atttggcggg taacgcggtt ctgtaccaga
     4201 tatttgcctt cgagctgttt gacagcggca taggaaaagg acatatcgcg ttcgtggtcg
     4261 atataggcgt tcagttcgtc aaattcttcg cggctgtaat cctcaaggat atgcctgtcg
     4321 tattttccgg catcggtaag ttttttaacg tggtcgtaaa ggtgcggcgg ctcgtactcg
     4381 ccgtaggcta ttttacgaag atggaaaatc gccaaacgcg cggcaaggta ttggtagtcc
     4441 ggggtatctt ccgaaattaa atcggcagcg gctttgatga tggtttcgtg gatgtcgtcg
     4501 gtgcggatgc cgttgtagaa ctggatgtgc gatttcaact cgacctgaga tacggaaaca
     4561 ttttccaatc cgtccgccgc ccaagtgacg acacggtgaa tcttatccaa atcaatggct
     4621 tctaatcttc cgtctcgttt ggttactttc aaatcagtcg gtgtattcat cgcttcctct
     4681 tccactcttg atattcaaga cacagtcttt tcaaataaat taaggcagac aatatagtgg
     4741 atttttggca ttttctccag tcttgacaac ggttgtattt ttcagtttca ggcaatgcgc
     4801 gataaaaccc gcctgtcgta tttttcctat aatatttgtt ttatctgata attctttacc
     4861 gataaaaaac gggtaaattt tttgcctttt gaccggctcc ggctacaagg cggtgaaata
     4921 aggattttcc gacgaaaaag gaaagcttcc tgttttctgc ccctgcaaat tgttaaattt
     4981 tgcaaagtat gattttgccg cgccgccgcc gacaaattcc attttcttac cgattggaat
     5041 ttattattga gattaatgtg ttatttgaat ctgcatatca aacggcaagt tttgtcggct
     5101 gaatgagtct gaaactgccg acaatttgcc cgttccatcc ttccatccta tactggaaag
     5161 aatgacaaac tgaaaggatc gtcatgtctg tcaaaaagat gcttgccata ctgttgtctg
     5221 caatattggg actggtatcg acaactgccg ctgccggtac gtcagaaccc gcccaccgcg
     5281 ataccaaaca tatccgcaag gcaaacaagc agatgctgca ccccgaatgc aggaaatatt
     5341 tggaacgccg tgccgcgtgg taccgatcgc aaggcaacgt gcaggaattg cgcgaaaaca
     5401 aaaaggcgcg caaagcattc cgctccctgc cttatgcgga acagaaaatc caatgccggg
     5461 cggcttatga ggctttcgat gatttcgacg gcggcagttt ccgccgttaa tcccatataa
     5521 aatatgccgt ctgaacacag gttcagacgg cattttccat agatacaaca aactatcctt
     5581 acggcatttc gtttgaggtg ctcccgttgc cgcagcct
//
LOCUS       DAWKST010000129         5586 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.129, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000129 DAWKST010000000
VERSION     DAWKST010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.129"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            72..998
                     /gene="fabD"
                     /locus_tag="WJL87_001614"
     CDS             72..998
                     /gene="fabD"
                     /locus_tag="WJL87_001614"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ7800025.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEHAIVKNTFAEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LNFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVKQICAEAAQGEVVEAVNFN
                     SPGQIVIAGNAAAVGRAMAAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQVAAFIEAH"
     gene            1136..3001
                     /gene="msbA"
                     /locus_tag="WJL87_001615"
     CDS             1136..3001
                     /gene="msbA"
                     /locus_tag="WJL87_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ7800026.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAINRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDGFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDASPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            3101..4666
                     /gene="guaA"
                     /locus_tag="WJL87_001616"
     CDS             3101..4666
                     /gene="guaA"
                     /locus_tag="WJL87_001616"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ7800027.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDGEPNTLDVWMSHGDKVSKLPDGFAVIGNTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPSWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMEKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            4728..5474
                     /gene="fabG"
                     /locus_tag="WJL87_001617"
     CDS             4728..5474
                     /gene="fabG"
                     /locus_tag="WJL87_001617"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ7800028.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         1348 a         1790 c         1370 g         1078 t
ORIGIN      
        1 ttttgtcccg cctgaatttt tcgtaaaact cgggcagaat acctgattat ccaaccaaac
       61 aaaggaatac tatgtctttt gccttctttt ttcccggaca aggttcccaa agcctcggta
      121 tgatgaacgg ctttgccgaa cacgccatcg tcaaaaacac ctttgccgaa gcctccgcca
      181 tattggggca ggacttgtgg gcgatgatta acggcagcga tgccgaaatc atcggacaaa
      241 ccgtcaatac ccagcccatc atgctcgccg ccggcgttgc cgtttaccgc gcctatttag
      301 aagcgggcgg caaaacgcct gccgccgttg ccggacacag cctcggcgaa tacaccgcac
      361 tcgttgccgc cggcgcattg aattttgccg acgcggtcaa actcgtgcgc ctgcgcgccg
      421 aactgatgca gtccgccgta ccgcaaggcg tgggcgcaat ggcggcgatt ctcggcttgg
      481 aagatgagca ggtaaaacaa atttgcgccg aagccgccca aggcgaagtg gtcgaagccg
      541 tcaacttcaa ctcacccgga caaatcgtga ttgcaggcaa tgccgccgcc gtcggacgcg
      601 ccatggctgc cgccaaagaa gccggtgcca aacgcgccct gccgctgccc gtgtccgtac
      661 cttcccattg cagcctgatg aaacccgccg ccgacaaact cgccgaagcc ctcaaaaccg
      721 tcgaaatcaa gcagccgcaa atccgcgtca tccacaacgc cgacgttgcc gcctacgatg
      781 atgccgacaa aatcaaagac gcgctcgtcc gccagcttta cagccccgta cgctggacgg
      841 aaaccgtcaa cgcccttgtt tcagacggca ttgccgaatc cgccgaatgc ggcccgggca
      901 aagtgttggc gggcttggca aaacgcatca acaaagccgc cgcgtgcagc gcactgaccg
      961 atgccggaca ggttgccgcc tttatcgaag cgcactgact tcgttctgca aaaagcagcc
     1021 tgccctcttc aggctgcttt tcatgtccga acggcggcag ccccatattt acgctataat
     1081 ccatcccgac caaaccaccg acagcggctg ccgttgcagt tcccgcccta ccgatatgat
     1141 agaaaaactg actttcggac tgtttaaaaa agaagacgcg cgcagcttta tgcgcctgat
     1201 ggcgtacgtc cgcccctaca aaatccgcat cgttgccgcc ctgattgcca ttttcggcgt
     1261 tgccgccacc gaaagctacc ttgccgcctt catcgccccc ctgattaacc acggcttttc
     1321 cgcacctgcc gcgccgcccg agctgtctgc cgccgccggc atcatttcca ccctgcaaaa
     1381 ctggcgcgaa cagtttacct atatggtttg ggggacggaa aacaaaatct ggaccgtccc
     1441 gctctttctc atcattctcg tcgtcatccg tggcatctgc cgctttacca gcacctatct
     1501 gatgacttgg gtctccgtga tgaccatcag caaaatccgc aaagatatgt ttgccaaaat
     1561 gctgaccctt tcctcccgct accatcagga aacgccgtcc ggcaccgtac tgatgaatat
     1621 gctcaacctg accgaacagt cggtcagcaa cgccagcgac atcttcaccg tcctcacgcg
     1681 cgacacgatg atcgttaccg gcctgaccat cgtcctgctt tacctcaact ggcagctcag
     1741 ccttatcgtc gtcctgatgt tccccctgct ctccctgctc tcgcgctact accgcgaccg
     1801 tctgaaacac gtcatttccg actcgcaaaa aagcataggc acgatgaaca acgtgattgc
     1861 cgaaacccat cagggacacc gcgtcgtcaa gctgttcaac gggcaggcgc aggcggcaaa
     1921 ccggttcgac gcgatcaacc gcaccatcgt ccgcctcagc aaaaaaatca cgcaggcaac
     1981 ggcggcacat tccccgttca gcgaactaat cgcctcgatc gccctcgccg tcgtcatctt
     2041 catcgccctg tggcaaagcc aaaacggcta caccaccatc ggcgaattta tggcattcat
     2101 cgtcgcgatg ctgcaaatgt acgcccccat caaaagcctt gccaacatta gcatccctat
     2161 gcagacgatg ttcctcgccg ccgacggcgt atgtgcattt ctcgacaccc cgcccgaaca
     2221 ggacaaaggc acgctcgcac cgcagcgtgt cgaagggcgc atcagcttcc gcaacgtcga
     2281 tgtcgaatac cgttcggacg gcatcaaagc cctcgacggc ttcaacctcg acatcagaca
     2341 aggcgaacgc gtcgccctgg tcggacgttc cggcagcggc aaatccaccg tcgtcaacct
     2401 gctgccccgc tttgtcgaac cgtctgccgg caacatctgc atagacggta tcgacatcgc
     2461 cgacatcaaa ctcgactgcc tgcgcgccca atttgccctc gtctcccaag acgtattcct
     2521 gtttgacgac accctgtttg aaaacgtccg atatagccgt cccgacgcgg gcgaagccga
     2581 agtcctatcc gccctccaag ccgctaacct gcaaagcctg atcgacgcat caccgctcgg
     2641 actgcaccag cccatcggat cgaacggcag caacttatcc ggcggacagc ggcaacgcgt
     2701 tgccattgcc cgcgccattt tgaaagacgc gccgatatta ttattggacg aagccaccag
     2761 cgcattggac aacgaatccg aacgcctcgt ccaacaggcg ctcgaacgcc tgatggaaaa
     2821 ccgcaccggc atcatcgtcg cccaccgcct gaccaccatc gaaggggccg accgcatcat
     2881 cgtaatggac gacggcaaaa tcatcgaaca aggcacacac gaacaactga tgtcccaaaa
     2941 cggctactac acgatgttac gcaatatctc aaacaaagat gccgccgtcc ggacggcata
     3001 aacaaaatgc cgtctgaaat ggtacaatcg ccccgaccct ttcagacggc atcatatccg
     3061 ccgacccatc cgattatctt caatcaccgt aaaacccatt atgacccaag acaaaatcct
     3121 catcctcgac ttcggttctc aagttaccca gctgattgcc cgccgcgtgc gcgaagccca
     3181 cgtttactgc gaactgcatt ccttcgatat gcctttggac gaaatcaaag ccttcaaccc
     3241 caaaggcatc atcctttccg gcggccctaa ttccgtttac gaatccgact atcaggccga
     3301 taccggtatt tttgatttgg gcattccggt tttgggcatc tgttacggca tgcagtttat
     3361 ggcgcaccac ttgggcggcg aagtgcagcc cggcaaccag cgcgaattcg gttatgcgca
     3421 agttaaaacc atagacagcg agctgacacg cggcattcaa gatggtgagc caaacacact
     3481 cgacgtatgg atgagccacg gcgacaaagt gtccaaactg cccgacggtt tcgccgtcat
     3541 cggcaacacc ccgtcctgcc cgattgccat gatggaaaac gccgaaaaac aattctacgg
     3601 catccagttc caccccgaag ttacccacac caaacaaggc cgcgccctgt tgaaccgctt
     3661 tgtcttggat atttgcggcg cgcaacctag ctggacgatg ccgaactaca tcgaagaagc
     3721 cgttgccaaa atccgcgaac aggtcggcag cgacgaagtg attttgggtt tgtccggcgg
     3781 cgtggactct tccgtagccg ccgcgctgat tcaccgcgcc atcggagacc aactgacctg
     3841 cgtgttcgtc gatcacggcc tcttgcgctt gaacgaaggc aaaatggtga tggatatgtt
     3901 cgcccgcaat ttgggtgtga aagtgattca cgtcgatgcc gaagggcagt ttatggagaa
     3961 actcgccggc gttaccgatc ccgagaaaaa acgcaaaatc atcggtgcgg aatttatcga
     4021 agtatttgat gctgaagaaa aaaaactcac caacgccaaa tggctggcgc aaggcacgat
     4081 ttaccctgac gtaatcgaat ccgcaggcgc gaaaaccaaa aaagcccacg ccatcaaatc
     4141 ccaccacaat gtcggcggcc tgcctgaaaa catgaagctc aagctgcttg agccgttgcg
     4201 cgatttgttc aaagacgaag tgcgcgaact gggcgtggct ttgggtctgc cgcgcgaaat
     4261 ggtgtaccgc catcccttcc ccggtcccgg tttgggcgtg cgcatcttgg gcgaagtgaa
     4321 aaaagaatac gccgacttat tgcgtcaagc agacgatatt tttattcaag aattacgcaa
     4381 caccaccgac gaaaacggca cgtcttggta cgacctgacc agccaagcct tcgccgtgtt
     4441 cctgcctgtg aaatccgtcg gcgtaatggg cgacggacgc acttacgact acgtcgtcgc
     4501 actgcgcgca gtcatcacca gcgactttat gaccgcacat tgggctgagt tgccttactc
     4561 actgctcgga cgcgtgtcca accgcatcat caacgaagtc aaaggcatca accgcgtggt
     4621 gtacgatgtc agcggcaaac cgcctgccac catcgagtgg gaataaccag caaacagggc
     4681 tgcaccgtcc ggcgcagtct ttcgattatc ggaaaaaagg aaaaaatatg agcacacaag
     4741 atttaagcgg caaaatcgct ttggtaacag gcgcctcgcg cggtatcggt gcagcaattg
     4801 ccgacacact ggcggcagcc ggtgccaaag tcatcggtac ggcgaccagt gagagcggtg
     4861 cggcggcgat tagcgagcgg ttggcgcaat ggggcggcga aggccgcgta ttaaattccg
     4921 ccgaacccga aaccgtcgaa aacctgattg ccgacatcga aaaaacgttc ggcaaactcg
     4981 atattctggt caacaacgcc ggcatcaccc gcgacaacct cctgatgcgt atgaaagaag
     5041 aagagtggga cgacatcatg caggtcaacc tcaaatccgt ctttcgcgcc tccaaagccg
     5101 tcttgcgcgg catgatgaag cagcgcgccg gccgtatcat caacatcaca tccgtcgtcg
     5161 gcgtgatggg caatgccggt caaaccaact atgccgcggc aaaagcaggc ttaatcggtt
     5221 tctccaaatc catggcgcgc gaagtcggca gccggggcat taccgtcaac tgcgtcgccc
     5281 ccggctttat cgataccgac atgacccgcg ccctgccgga agaaacccgc caaaccttta
     5341 ccgcccaaac cgccttgggc agattcggcg acgcgcaaga cattgccgat gcagtcttgt
     5401 tcttggcttc cgatcaggca aaatacatta ccggtcaaac gctgcacgtc aacggcggca
     5461 tgctgatgcc ctgacgggca gccgtaccga caatgccgtc tgaagccctt tcagacggca
     5521 tttgcattct caggcaaaat gaacacacgc cacaccccgc cctgcccctg cggatcaggc
     5581 acaagc
//
LOCUS       DAWKST010000130         5534 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.130, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000130 DAWKST010000000
VERSION     DAWKST010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.130"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..77)
                     /locus_tag="WJL87_001618"
     CDS             complement(<1..77)
                     /locus_tag="WJL87_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ7800029.1"
                     /translation="MRKSRLSQYKQNKLIELFVAGVTART"
     gene            complement(383..1642)
                     /gene="rho"
                     /locus_tag="WJL87_001619"
     CDS             complement(383..1642)
                     /gene="rho"
                     /locus_tag="WJL87_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ7800030.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            complement(1862..4246)
                     /gene="ppsA"
                     /locus_tag="WJL87_001620"
     CDS             complement(1862..4246)
                     /gene="ppsA"
                     /locus_tag="WJL87_001620"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ7800031.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGNRNLRRFAINGDKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4656..5477
                     /locus_tag="WJL87_001621"
     CDS             4656..5477
                     /locus_tag="WJL87_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ7800032.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
BASE COUNT         1159 a         1292 c         1625 g         1458 t
ORIGIN      
        1 gttcttgcag ttacacctgc gacaaacagt tcaatgagtt tattttgttt atactggctt
       61 agacgacttt ttctcatagg ggcaactcta acttaatttg aatttcccta gttatctagg
      121 acagccccaa atttatacaa aaatgagcgc ggttcggcgc aaccttgaat caagttcccg
      181 catcggtttt cattgccggt acggatgcgt tcaagccggt tttgcaaagg ccgcgctttc
      241 ggcaagcgga cacggacact gccgacggtt gcgccgttaa cggggggagg gaggagctgc
      301 gccgaccgtg tgaatgaaag tgccgtctga aacccgattt tcaggcttca gacggcattt
      361 cgcattaatg cgggcggcgc gtttatttgc cgcgcatcag ttcaaagaaa tcgtcgttgt
      421 ttttagaggc tttgattttc ccgtttaaga actcggtagc ttcgatttca tccatcgggt
      481 gcaggaactt gcgtaagagc cacatacgtt gcaactggtc gttcggcaca agcagctctt
      541 cgcggcgcgt gccggatttg ttgatgttga tggcggggaa gaggcgtttt tccgccatac
      601 ggcgatcaag gtgcaattcc atattgccgg tgcctttgaa ttcttcgtaa atcacatcgt
      661 ccatacggct gccggtttca accaatgcgg tggcgataat ggtcagcgaa ccgccttctt
      721 ccacgttgcg cgccgcaccg aagaaacgtt tggggcggtg cagcgcgttg gcatcgacac
      781 cgccggtcag gattttgccc gaggtgggga cgacggtatt gtaggcgcgg gcaaggcggg
      841 taatcgaatc cagcaggatg accacgtctt ttttgtgttc caccatacgc ttggcttttt
      901 caagcaccat ttcggcaact tggacatggc gttgagccgg ctcgtcaaag gtggaggaaa
      961 ccacttcgcc acggacggag cggctcattt cggttacttc ttcgggacgt tcgtcaatca
     1021 agaggacgat gagttcgact tcgggatagt ttgcggtaac ggcgtgggca atgttttgca
     1081 gcatcacggt tttaccgctt ttgggcgggg caaccaagag ggcgcgctga cctttgccga
     1141 tgggggaaat caggtcgatg gcgcgtccgg tcaggttttc ttcggacttt aagtcgcgtt
     1201 ccagcttcaa ctgttcggtc ggaaacagcg gggtcaggtt ttcaaacagg attttatggc
     1261 ggcatacttc cgggtggtcg ccgttgatgg tatcaagcct gaccagggca aaatagcgtt
     1321 cgttgtcttt tgggacgcgg acgctgcctt cgatggtgtc gcccgtatgc aggttgaagc
     1381 ggcggatttg ggtgggcgag acatagatgt cgtcggggcc ggcaagatag gacgtgtccg
     1441 cgctgcggag gaagccgaag ccgtcgggca ggatttcaag cgtgccggag caggtgaaac
     1501 cctcgccttt tttcatcatc tggcggacga tggcaaatac gaggtcttgt ttgcggaatc
     1561 ggttggcgtt ttcgatgccg tgttcttccg ccaattctaa gagtttggaa atgtgcaggg
     1621 tttgtaattc ggagacgtgc ataataatga tgtattttga agaggaaaaa gacaggcaga
     1681 tgccgtctga aagaagaagc tgaccgttgc cggttgctcg gggaaggggg aattgtaggc
     1741 agtcggcgcg tgggtgtcaa atattatcgc ggacggggca tcggcaggaa atgccgtctg
     1801 agcggagctg cttggaaaaa aatacccccg cgcttttcag gctcgggggt atgggcattg
     1861 attatttgtt caattcattc gccaaatata gccaagtttc gatgacggta tccgggttca
     1921 gggaaacgct ttcaatgcct tcctcaacca gccatttggc gaagtccgga tggtcggacg
     1981 ggccttgacc gcagatgccg acatatttgt tctgcttgcg gcaggcggag atggcaagat
     2041 gcagcatcac tttgacggca gggttgcgtt cgtcaaacga ttcggatacc aagccgctgt
     2101 cgcggtcgag accgagggtc agttgggtca tgtcgttcga gccgatggag aagccgtcga
     2161 agtattgcag gaattgttcc gccaataccg cgttgctcgg cagctcgcac atcataatca
     2221 ggcgcaggcc gtttttgccg cgttccaagc cgttttcttt cagggctttg acaacggctt
     2281 cggcttcgcc caaagtgcgg acgaacggaa tcatgatttc aacgttggtc agacccattt
     2341 cgtcacgaac gcgtttcaag gctttgcatt ccaaggcgaa acagtctttg aagttgtcgg
     2401 cgacataacg cgccgcgccc cggaagccca acatcgggtt ttcttcatgc ggttcgtata
     2461 cgttgccgcc gactaggttg gcgtattcgt tggatttgaa gtcggacata cggacgatgg
     2521 ttttacgcgg ataaaccgat gcggccaatg tcgccacgcc ttcggcgatt ttatcgacgt
     2581 agaagtcgac aggggacgcg taaccggcga tacggcgggt aatttccgct tttaattcgt
     2641 cgtcttgttt gtcaaattcc aacaaggctt tcgggtggat accgatttgg cggttgatga
     2701 taaattccat acgcgccaag ccgatgcctt cgctgggcag gttggcgaag ctgaatgcga
     2761 gttcggggtt gccgacgttc atcatgactt tgacgggtgc tttgggcata ttgtccaagg
     2821 cgacatcggt aatctgtacg tccaacaggc cggcatagat gaagccggta tcgccttcgg
     2881 cacaggatac ggtaacttct tgaccgtttt tcagcaattc ggttgcattg ccgcagccga
     2941 caacggcagg aatgcccagt tcgcgcgcga tgatggcggc gtggcaggta cggccgccgc
     3001 ggttggtcac gatggcggaa gcacgtttca tcaccggttc ccaatctggg tcggtcatgt
     3061 cggtaacgag tacgtcgccg gcttcgacgg aatccatctc ggaagcatct ttaatcaggc
     3121 gcaccttgcc ctgaccgact ttctgaccga tggcgcggcc ttcgcataat acggttttgt
     3181 cgccgttgat ggcgaagcgg cgcaggttgc ggttgccctc ttcttgggat tttacggttt
     3241 cgggacgggc ttgcaggatg tagagtttgc cgtccaagcc gtcgcgtccc cattcgatat
     3301 ccatcgggcg gccgtagtgt ttttcgatgg tcagtgcgta atgcgccaac tcggtgattt
     3361 cttcgtcggt aatggagaag cggttgcggt cttcttcggg gacatcgacg ttggttaccg
     3421 atttgccggc ttctgctttg tcggtaaaaa tcattttgat gtgttttgaa cccatggttt
     3481 tacgcaggat ggcgggtttg cctgctttca gtgtgggttt gaatacatag aattcgtcgg
     3541 ggttgaccgc gccttggacg acgttttcac ctagaccgta agaggaggta acaaagacga
     3601 cttgatcgta gccggattcg gtgtcgagag tgaacatcac gccggatgcg ccgctgtcgg
     3661 aacgcaccat gcgttgaacg ccggcggaaa gggcgacgat atcgtgttcg aagcctttgt
     3721 ggacgcggta ggaaatggcg cggtcgttgt acagggaggc gaatacatgg tgcatcgctt
     3781 ctttaacgtt ttccaatccg ttgatgttca agaaggtttc ctgttggccg gcaaatgatg
     3841 cgtcgggcag gtcttcggcg gttgcggaag agcgtacggc aacggaaatg tccgcaccgc
     3901 cggcatcggc aaccattttg ttccatgccg cttcgatttc ggcatcgagc tgttcgggga
     3961 agggcgtgtc caaaatccat tggcggattt ctttgccgac gcgtgccagt tcggcaacgt
     4021 cttcgacatc taattttgcc agtgcggcgg aaatgcgttc gctcagaccg ttgtgtgcga
     4081 ggaatgcgcg gtaggcatcg gctgtggtgg caaagccgcc ggggacgcga acgccttttt
     4141 cggtcagctg actgatcatt tcgcccagcg aggcgttttt accgcccacg cgttcaacat
     4201 ctgtcatacg caggttttca aaccagatta cgtagttgtc ggccatttgt gtgtccaatc
     4261 caaaatatgt taaaaaagaa acaaatccgc gtgcttattt taagcgattc gttcctctgc
     4321 tgtcatgtgt tttatccgtt ttaaaatcat gatgccgtct gaaaaattgc ggtttcggcg
     4381 tgtgtagcgg tttgaaactt gcagccggta tacttctttt tttgggtatt ttctttgtaa
     4441 aacaggtggt ttgaataggt taatgttttt tctgtttgat tttttgttta ttttttaaaa
     4501 ttttctgcca aaaaatactt tatataaata attttatttc aaaattatat tgtgtctgtt
     4561 tgggtgtaat ccgaggtagg cgtgctgcgg gtgctttcct tgtgtctgcc gctgctgtta
     4621 tgatgatatt tttattctgt atttaaggat ggaagatgag cagtccgcgc catgtgtttt
     4681 acatttccga ccgtaccggt ctgactgccg agaatatcgg cgaggctttg ctgaaccagt
     4741 ttggcaatct gtcgttcaaa cgccatacgc atccgtttgt cgatacgccg gaaaaggcgc
     4801 gcgcggtggt ggagaaggtc aatcggagcc ggcaggaaaa cggtcagcgt ccgattgcgt
     4861 ttgtcagtgt ggttgatgac gaaatccgtc ggattatcaa aggggcggat gcttttcaga
     4921 ttaatttctt tgagactttt ttgggactgt tggagaaaga actcaatacc gaagccacgg
     4981 catccgagca ggggcatcac agtatcggta atacgaagcg ttacgatgcg cgtatggagg
     5041 cggtaaattt ctcattgaac cacgatgacg gggtcagcga taagaacctt caggaagcgg
     5101 atgtaatctt gatgggtgta tcgcgttcgg gcaaaacgcc gacctgcctt tacctcgccc
     5161 tgcaatacgg catccgtgcg gcaaactatc cgctgattcc tgacgatttg gaatcggccg
     5221 atctgccgcg tatggtcaag ccttataggg ataagctgtt cgggttgacc atccagccgg
     5281 aacgtttgca ggccatccgc caagagcgcc gcccgaattc aacttatgcc aaaatcgata
     5341 catgccgcag cgaggtggca gacgcgcaga gtatgttcag acggcatggg attccgtttg
     5401 cgaatacgac ggataaatca gtggaagagc ttgccgtaca catccttcag gcgtgcaagc
     5461 tcaaacgcag gttttgacgg gctttgattc ggtttgaagg cggaactgcc gtctgaaatc
     5521 aggtttcaga cggc
//
LOCUS       DAWKST010000131         5534 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.131, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000131 DAWKST010000000
VERSION     DAWKST010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.131"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            208..1641
                     /gene="lpdA"
                     /locus_tag="WJL87_001622"
     CDS             208..1641
                     /gene="lpdA"
                     /locus_tag="WJL87_001622"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ7800033.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            1736..2023
                     /locus_tag="WJL87_001623"
     CDS             1736..2023
                     /locus_tag="WJL87_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800034.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            2122..3288
                     /gene="sucC"
                     /locus_tag="WJL87_001624"
     CDS             2122..3288
                     /gene="sucC"
                     /locus_tag="WJL87_001624"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ7800035.1"
                     /translation="MNLHEYQAKELLASYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVQGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            3299..4189
                     /gene="sucD"
                     /locus_tag="WJL87_001625"
     CDS             3299..4189
                     /gene="sucD"
                     /locus_tag="WJL87_001625"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ7800036.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPSPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(4491..4829)
                     /locus_tag="WJL87_001626"
     CDS             complement(4491..4829)
                     /locus_tag="WJL87_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7800037.1"
                     /translation="MDVQKQYPFETVAVCVLPNHIHAIWTLPPDDADYSLLRRLIKTK
                     FSAYSPHTKNLSAGKQRRNERGIWQRRFYEHTVRDETDLQRCADYIHSNPIKQKMSRA
                     KTESCKNIIN"
     gene            complement(5159..>5534)
                     /locus_tag="WJL87_001627"
     CDS             complement(5159..>5534)
                     /locus_tag="WJL87_001627"
                     /inference="COORDINATES: protein motif:HMM:NF024685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEZ7800038.1"
                     /translation="SNIIFTYLDYAQARVESAIFYEVLKSLALTHTFLFIGCGINDPD
                     IAIIFEDIRFAHGDDLPEHYMTIPKEEVDTDIMNLVSSMRNIHFCEYDSTDGHSQLTK
                     ALIDLRYAVEERRNEIAKNQKW"
BASE COUNT         1494 a         1294 c         1395 g         1351 t
ORIGIN      
        1 ttagatgtag cgtaatgtag tatcgtgcta caataggctc aacgaacgat tgaggccgtc
       61 tgaaacattt gattcgaatg aatcggcaga tatggacttt cagacggctt tttcttaaaa
      121 ccatcaaaac gcagtcattc aaaataaaaa agaaacgaaa agtatcgttt ttattttgag
      181 atactgctaa aagcaaagga tgacacgatg tctcaatatg atgtagtagt gattggtgca
      241 ggtccgggcg gatacgttgc cgccatccgt gccgcacaac tgggtttcaa aactgcctgt
      301 gtcgatgcag gcgttaacaa agcaggcaat gcccctgcct tgggcggtac ttgcttgaac
      361 gtaggttgta tcccttctaa agccctgttg caatccagcg aacatttcca cgctgcgcaa
      421 cacgattttg ccgaacacgg tatcactgtc ggtgacgtaa aattcgacgc ggccaaaatg
      481 attgagcgca aagatgccat cgtgaccaaa ctgaccggcg gcgtgaaatt cctgttccaa
      541 aaaaataaag taaccagtct gttcggtacg gcttcctttg ccggtaaaaa tggcgatgct
      601 taccaaatcg aagtcgataa caaaggagag aaaaccgtta tcgaagccaa acacgtcatc
      661 gtagcgaccg gttccgtacc gcgtccgctg ccacaagtcg ctatcgacaa tgtgaacgta
      721 ttggacaacg aaggcgcatt gaacctgact gaagtacctg ccaaactcgg cgtgatcggt
      781 tccggtgtga ttggtttgga aatgggttcc gtatggaacc gcgtgggtgc ggaagttacc
      841 attcttgaag ccgcgccgac cttcctggct gccgccgacc aacaaatcgc caaagaagcc
      901 ttcaaatact tcaccaaaga gcaaggtctg agcatcgaat tgggcgtgaa aatcggcgac
      961 atcaagtctg aaggcaaagg tgtttccgtt gcttacgaaa ctgctgctgg cgaagccaaa
     1021 accgaagtat tcgacaaact gatcgttgcc atcggccgta ttccaaacac caaaggcctg
     1081 aacgcggaag ccgtaggctt ggaaaaagac gagcgcggct ttatcaaagt agatggcgaa
     1141 tgccgtacca acctgcctaa cgtatgggca atcggcgacg tggttcgcgg cccgatgttg
     1201 gcacacaaag ccagcgacga aggcgttgcc gttgccgaac gcattgccgg tcaaaaaccg
     1261 catatcgact tcaacaacgt accgttcgtg atttacaccg atcctgaaat cgcttgggtg
     1321 ggtaaaaccg aagagcagct caaagccgaa ggcgtggagt acaaaaaagg tacttcaggt
     1381 tttggtgcga atggtcgcgc attggcaatg ggcaaagcca aaggtacggt taaagtgttg
     1441 gcagacgcca aaaccgaccg catcttgggc gtacacatga ttggtccggt tgtcagcgaa
     1501 ttggttaccg aaggcgtgac tgcgctcgaa ttcttcgcca gcagcgaaga catcgcccgc
     1561 attatccatg cccacccaac cttgtccgaa gtggttcacg aagctgcatt ggcggccgac
     1621 aaacgcgctt tgcacggttg atagacatta aggccttcga caaattgaat gttccgagag
     1681 ctccgttttc tgatttataa ttccgtcaga caaacaaaca gcatttacat tcgttatgaa
     1741 caaagaaata gtcggtattt tctttatacc gatgggcatc atcagcatgt gtatggccgc
     1801 attgtggcag atgtatgtga tgatgaccga aacttatacg ctcaaccgtt tcaaagataa
     1861 agaattggtt tggcgcgtgg cattgttgtt tatcagtttc agccttgccg tttatctgct
     1921 ctgtccgaat tcgcgtaaaa aaggcatcgt cttttttatt ctcgggggag gcggtgcagc
     1981 catgtatctg ctggcgcgga tgtggttgcc tttcagcaag tgaaacgacg attttccgac
     2041 cgccgaaagg tagtctgaaa cgcacgggct tgccatttgg aggcaaactc ggggcattcc
     2101 actaatctaa aggagaaatc catgaattta cacgagtatc aggctaaaga actgctggct
     2161 agctacggtt tgcccgtaca aggcggtatt ttggcacaca acggcgaaga agccgctgca
     2221 gcttacgaca aattgggcgg caaattcgct gttgtcaaag cacaagtaca cgccggcggc
     2281 cgcggtaaag cgggcggcgt aaaagtcgtt aaaagccgcg aagaagctaa agaagtggct
     2341 gaaagcctga ttggcaccaa cttggtaact taccaaacgg atgccaacgg ccaacctgtc
     2401 aacagcgttt tggtttgtga agatatgtat ccggttcaaa ccgagctgta cttgggcgca
     2461 gtggttgacc gttctacccg ccgcgttaca ttcatggctt ctacagaagg tggcgtagaa
     2521 atcgaaaaag ttgctgccga aactccagaa aaaatcttca aagtaaccgt tgatccgctg
     2581 gtcggcctgc aaccttgcca agctcgcgaa gttgccttcc aactgggctt gaaagacaaa
     2641 caaatcaacg agttcgtcaa actgatgacc ggcgcgtaca aagcgtttgt cgaaaacgac
     2701 ttcgccctgt ttgaagtcaa cccgctggca gttcgcgaaa acggcgcact ggcttgcgtg
     2761 gacggcaaaa tcggcatcga cagcaacgcg ctctaccgcc tgccaaaaat cgctgaattg
     2821 cgcgacaaat ctcaagaaaa cgaacgtgaa ctgaaagctt ctgaattcga cctgaactat
     2881 gttgccctgg aaggcaacat cggctgtatg gttaacggtg ccggtttggc gatggccacc
     2941 atggacatca tcaaactgaa aggcggccaa cctgccaact tcttggacgt tggcggcggc
     3001 gcaaccaaag accgcgtggt tgaagcgttc aaactgattc tggaagacaa atccgttcaa
     3061 ggcgtattga tcaacatctt cggcggtatc gtacgttgcg acatgattgc ggaagccatc
     3121 gtggcagccg ttaaagaaat caacgtcgac gttcctgtcg ttgttcgttt ggaaggcaac
     3181 aacgccgaac tcggcgcgaa aatcctgaac gaatcaggtc tgaaactgac ttctgcagac
     3241 ggcctgaatg acgcagccga aaaaattgtt gcagccgtaa acgcctaagg agaaaagaat
     3301 gagcgtattg attaataaag acaccaaagt attggttcaa ggtttcaccg gtaaaaacgg
     3361 tactttccac tccgaacaag ctctggctta cggcactaaa gttgtcggcg gcgttacccc
     3421 gggcaaaggc ggtcaaaccc acctgaacct gcctgtattc aacaccatga aagaagcggt
     3481 taaagaaacc ggcgcggatg catctgtaat ttacgttccc tctccgtttg tgttggattc
     3541 tatcgttgaa gccgttgatt caggcgtagg cttggtcgtt gtgattaccg aaggcgtacc
     3601 gactctggac atgctcaaag ccaaacgtta tttggaaacc aacggcaacg gcacacgttt
     3661 ggtgggccct aactgcccgg gcgtgattac cccaggcgag tgcaaaatcg gtattatgcc
     3721 gggccacatc cacactccgg gccgcatcgg tattatttcc cgttccggta cattgactta
     3781 cgaagctgtg gcacaaacca ccaaactggg cttgggtcaa tcaacctgta tcggtatcgg
     3841 cggcgacccg attcccggta tgaaccaaat cgacgcactg aaacttttcc aagaagaccc
     3901 ggataccgac gccatcatca tgatcggtga aatcggcggt actgcggaag aagaagcagc
     3961 cgaatacatc caatccaacg taaccaaacc tgttgtaggt tacattgctg gtgttaccgc
     4021 tcctaaaggc aaacgtatgg gccacgccgg tgcgattatc tccggcggta aaggtactgc
     4081 cgaggaaaaa ttcgccgctt tcgaaaaagc cggtatcgcc tacacccgca gccctgccga
     4141 gttgggcact accatgctgg aagtgttgaa ggcaaaaggt ttggcataat caggtttgac
     4201 aactgattaa acatcaaatg ccgtctgaaa ccggaaatcg ggtttcagac ggcattttgt
     4261 ttgtcatttc aaaaagaggc agcctcaaca tacccacatt attttttccc ctttgaggca
     4321 gtcagaatat ttatcggtca aacaggagta aacccgtaaa ttgcctcttg cactttattc
     4381 acctgagctc aacccgattg agaaagtgtg ggcgaatatt aagcggtatc tgcaaaccgt
     4441 tttgtctgat tacgcccgat ttgacgatgc actactgtcc tattttgatt ttaattgatt
     4501 atatttttgc aggattctgt tttagcacgc gacatttttt gtttgatagg attggaatgg
     4561 atgtagtccg cgcaacgctg caaatccgtt tcgtcgcgca cggtgtgttc gtaaaaacgc
     4621 cgttgccaaa taccgcgttc gttccgccgc tgtttgccgg cggataagtt tttagtatga
     4681 ggggaatatg cggagaattt ggttttaatc agccgccgga gtagggaata atccgcatcg
     4741 tcgggcggca gcgtccaaat ggcgtgaatg tggtttggca gcacgcatac ggcgacggtt
     4801 tcaaagggat attgtttttg cacatccata taagccgccc gcgaagtcga tatgtccgac
     4861 aagcaggtgc gatttcgggt cggcgggttt gacagtgaaa aagaatgtgc cgccggcaat
     4921 gaagtttctg cgataacgcg ccataggatt tttgtttgtg gttggggtgt tggagaagga
     4981 atagtttgtt gtttaaggtt ttaactttgt tggagctgat gttttcagac gacattggag
     5041 gccatctgaa atgttgaaac ggcaaaccgt caatcaagca atcgcgtgcg tgcgtaccgc
     5101 acacacccta cgcatggggt ttagatttta tacaggctac ggcttgcttt tcgatcgatt
     5161 accatttttg atttttagca atttcatttc gtcgttcttc tactgcataa cgaagatcaa
     5221 tcaacgcttt agtcaattga ctgtgtccat cagtagaatc atattcacaa aaatgtatgt
     5281 ttctcatgga gctgactaga ttcattatat cagtatcaac ttcttccttg gggattgtca
     5341 tgtaatgctc aggcaaatca tctccgtgtg cgaatcgaat gtcttcaaaa ataatcgcta
     5401 tatctgggtc attaatgcca cagccaatga ataaaaaagt atgggtaagt gcaagtgatt
     5461 ttaatacttc ataaaagatg gcagattcaa ctcgtgcttg agcataatct aagtaggtaa
     5521 aaattatatt agaa
//
LOCUS       DAWKST010000132         5525 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.132, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000132 DAWKST010000000
VERSION     DAWKST010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.132"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..489)
                     /locus_tag="WJL87_001628"
     CDS             complement(<1..489)
                     /locus_tag="WJL87_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800039.1"
                     /translation="MSSTISWDVSLIDALARRQAVVMLGAGVSRNSTNEHGMRPKTWK
                     DFLETCANQLDKPNVVKSLIDKNDFLTACELIKRSMARTKFDKLIKDEFNAGFVPAEI
                     HEEIFKLDQAIIISPNFDSIYETYARQKTNGSILVKSYKDKDIAKYIRGGDNRLIIKS
                     HGS"
     gene            complement(500..1501)
                     /locus_tag="WJL87_001629"
     CDS             complement(500..1501)
                     /locus_tag="WJL87_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ7800040.1"
                     /translation="MKSIGFFNNKGGVGKTTLLCNLAASLSIHFSKKVLVIDADPQCN
                     ASAYLLPENMLEDILMNDTESSIDKFYEPIRKGQGFPEKNPKIVRSDRFQIDLIVGNP
                     KLAIREDFIATDWNDTKNGEARGFQTTFAMAELFSRMSDYDFILIDMGPSLGALNRSI
                     LLSSDYFLMPISADIFSMMAISNIQISFDGWKEDLINALERYNKREGKKFTISNDIPF
                     EWKLDFLGYVRQQYKAKYSGGERQPVAAFERIINQQDEELKKLCLSFKHEDINSRKIG
                     EIHTLSSVIPLSQQAHAPIFELAASDGIVGGHYSRVAEAEEYFKKIAERFLENIGID"
     gene            complement(1580..4432)
                     /gene="uvrA"
                     /locus_tag="WJL87_001630"
     CDS             complement(1580..4432)
                     /gene="uvrA"
                     /locus_tag="WJL87_001630"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ7800041.1"
                     /translation="MCNHHPQHSHDNDTIRIRGARTHNLKNVDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHKLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMESGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLATGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPEKVKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVSGEPLHEVSAWPLTKTHQ
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCENSVTGQYLSGKKSIAVPSERTPVNPERMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIDRLKGKGNSIVIIEHNLDVIKTADYIVDLGP
                     EGGDGGGRIIAEGSPEQVAKVKGSYTGKYLEVMLKY"
     gene            4587..5384
                     /locus_tag="WJL87_001631"
     CDS             4587..5384
                     /locus_tag="WJL87_001631"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ7800042.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVSTVLARLPLTAP
                     QIESEPESEPALQTAPVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
BASE COUNT         1269 a         1250 c         1426 g         1580 t
ORIGIN      
        1 tgaaccgtga gattttataa ttaacctatt atctccgccc cgaatatact tagcgatatc
       61 tttatctttg tatgatttaa caagaataga gccgtttgtt ttttgtcttg catatgtttc
      121 ataaattgaa tcaaaatttg ggcttattat tatggcttga tcaagtttaa atatctcttc
      181 atgtatttca gcaggtacaa agccagcatt aaattcatct ttaattaatt tatcaaattt
      241 tgttctagcc atagatcgtt taattagctc gcaagctgtt aaaaaatcat ttttatcaat
      301 gagtgattta accacattgg gtttatcaag ttgattcgca caagtttcca agaaatcttt
      361 ccatgttttc ggtctcatgc catgttcatt tgttgaattt cttgatacac ctgcaccgag
      421 cataacaaca gcttgtctac gagctaaagc atctatgaga gacacatccc aacttatagt
      481 agatgacata atttcctctt taatcaatac ctatattttc caaaaatcgc tcagcgattt
      541 ttttaaaata ctcttctgcc tccgcaactc ttgaataatg tccccctaca ataccatcag
      601 atgcagctag ttcaaatata ggagcatgtg cctgctgtga tagtggtata acactactta
      661 atgtatgtat ttctccaatt tttctgctat tgatatcttc atgtttaaat gataggcata
      721 gtttttttaa ttcttcgtct tgttgattaa taattctttc aaaagctgca acaggctgtc
      781 tctccccacc tgagtatttg gctttgtatt gttgtctaac atatcctaag aaatctaatt
      841 tccattcaaa tggaatatca ttggagatgg taaatttttt accttctcgt ttattatacc
      901 tttcgagtgc atttatcaaa tcctctttcc atccatcaaa agaaatttgt atatttgaga
      961 ttgccatcat actaaaaata tcagcagaaa tcggcattaa aaaataatca gagcttagta
     1021 aaattgacct atttaaggca cctagtgatg gtcccatatc aatcaaaata aaatcgtagt
     1081 cactcattct gctaaatagc tcagccattg caaaagttgt ttgaaatcca cgagcctctc
     1141 cattttttgt gtcattccag tctgttgcga taaaatcttc cctgattgct aactttggat
     1201 ttccaactat taaatcaatt tggaaacgat cacttcttac aattttggga tttttttcag
     1261 gaaagccttg gccttttcta ataggctcat aaaatttatc aatactactt tcagtatcgt
     1321 tcattaatat atcttcaagc atattttctg gtagtaaata agctgaagca ttacactgtg
     1381 gatccgcatc aattactaaa acttttttac taaagtggat agataatgat gctgctaaat
     1441 tgcacaatag agtggttttg cccactcctc ctttgttatt aaaaaaacca atgcttttca
     1501 tgggatttta actttccaga aaaatgaaat cttgtaatta aatatttaat atttatagta
     1561 tatttggata gtatattgac taatatttta acataacttc caaatacttc ccggtataac
     1621 tccccttaac cttcgccacc tgctcgggac tgccttccgc aataatcctc cccccgccat
     1681 caccaccttc cggccctaag tccacaatat aatcagccgt ttttataacg tcaagattat
     1741 gctcgataat cactatcgag ttgcctttgc ctttcagacg gtctatgact tccagcagca
     1801 gggcgatgtc ggcgaagtgt aggccggtgg tgggttcgtc gaggatgtag agcgttctgc
     1861 cggtgtcgcg tttggagagt tccaaggcga gtttgacgcg ctgggcttcg ccgccggaga
     1921 gggtggtggc ggattggccg agacggatgt agcctaagcc tacgtccatc aaggtttgca
     1981 gtttgcgcga tacggtgggg acggcgtcga aaaattcgcg ggcttcttca acggtcatgt
     2041 cgaggacttg gctgatgttt ttgcctttgt attggatttc gagcgtttcg cggttgtagc
     2101 gtttgccgtg gcagacttcg cagggaacat acacgtcggg caggaagtgc atttcgactt
     2161 tgatgacgcc gtcgccttgg caggcttcgc agcggccgcc tttgacgttg aaggagaatc
     2221 tgccgacatt gtagccgcgt tcgcgcgaga ggggtacgcc ggcgaagagt tcgcggatgg
     2281 gggtgaacag gccggtgtag gtggcgggat tggagcgtgg ggtgcggccg atgggggatt
     2341 ggtcgacgtt gatgactttg tcgaggtgtt cgaggccgcg gatgtcgtcg tatggggcgg
     2401 gttcttcttg ggcgcggttg agttcgcggg cagtgatttt ggcgagggtg tcgttaatca
     2461 gggtggattt gccgctgccg gatacgccgg tgatgcaggt aattaaaccg agcggcagtt
     2521 cgagggtaac atttttgagg ttgttgccgc gcgcgccttt gaggacgagc attcgttcgg
     2581 gattgacggg cgtgcgttca gacggcacgg caatggattt tttgccgctg agatattgtc
     2641 cggtaacgga gttttcgcat ttggcgacgt tttcgggcgt gtcggcaatc aatacgttgc
     2701 cgccgtgttc gcctgcgccc gggcccatat cgacgacgaa atcggcttcg cggatggcgt
     2761 cttcgtcgtg ttcgaccacg atgacgctgt tgcccaaatc gcgcaggcgt ttgagggtgg
     2821 caagcaggcg gtcgttgtcg cgctggtgca ggccgatgga gggttcgtcc aaaacgtaca
     2881 tcacgccggt caggccgctg ccgatttggc tggcgaggcg gatgcgctgg gcttcgccgc
     2941 cggaaagggt ttcggcggaa cgggagagat tcaggtaatc cagcccgacg ttaatcagga
     3001 agccgaggcg ttcggtgatt tctttgagga ttttttcggc gatttgtttt ttgttgccgt
     3061 ctaaatccag tgtttcaaag aattggtggg ttttggtgag cggccaggcg gaaacttcgt
     3121 gcaacggctc accgctgacg taaacgtagc gggcttcttt gcgcaaacgt gcgccgccgc
     3181 agctcgggca ggcgcggtgg ttttggtatt cgcgcagttt ttcgcgcacg gtttcgctgt
     3241 cggtttcgcg gtagcggcgt tcgagattgg ggatgatgcc ttcgaaggcg tggctgcggt
     3301 tgaaggtggt gccgcgttcg gataggtagg tgaaatcaat gacttccttg cccgagccgt
     3361 gcagcacgac ttttttgact ttttcaggca gcgtttccca agcagcctgc acatcgaaac
     3421 cgtaatgccg cgccagcgac tgaatcattt ggaaatagaa ctggttgcgc ttatcccagc
     3481 cgtcaatcgc gcccgttgcc agcgacaatt cgggatgcgc gaccactttt tcgggatcga
     3541 agaagttggt gttgcccaag ccgtcgcaag ttgggcagga gcccatcggg ttgttgaacg
     3601 aaaacaggcg cggctctaat tcgggcaggc tgtatgaaca tacgggacag gcgaaacgcg
     3661 cggaaaacca atgttcttcg ccgctttcca tttccatcgc cagcgcgcgc tcgttgccgt
     3721 ggcgcagtgc ggtttcaaaa ctttccgcca gccgctgctt gatgtccgcc ttcactttca
     3781 cgcggtcgat gaccacgtcg atattgtgct tgatgttttt ctccagcttc ggcacttcgt
     3841 caagctgata aacttcgccg tccacgcgca cacgggcaaa gccttgcgcc tgcaagtcgg
     3901 caaagaagtc gacaaactcg cccttacgct ctcgtaccgc cggcgccaga atcatcacgc
     3961 gcgtgtcttc cggcagtttt aagacggcat caaccatttg agatacggtt tggctggata
     4021 gcggcagctt gtgttcgggg cagtagggtg tgccgacgcg tgcgtacaaa aggcgcagat
     4081 agtcgtggat ttccgtaacc gtgccgacgg tggaacgcgg attgtggctg gtggatttct
     4141 gctcgatgga aatcgcgggc gacaggcctt cgatcaaatc gacatcgggt ttgtccatca
     4201 tctgcaaaaa ctgccgcgca taggcggaca ggctctcgac ataacgccgc tgtccttcgg
     4261 catacagcgt gtcgaacgcc agcgatgatt tgccgctgcc tgacaatcct gttaccacca
     4321 ccagcttgtg gcgcggaatg tctaaatcga cgtttttcaa attatgcgtg cgcgcgccgc
     4381 gtatgcggat ggtgtcgttg tcgtgcgaat gttggggatg atggttgcac ataatggatg
     4441 ccgcctgaaa aataaaggaa aaccgatatt gtagcacttt atcaaataac gtcttaagca
     4501 ggattcagac ggcatttgtc ggcggagtac ggcagattcc gctataatgt cggcaatttt
     4561 aacccgcttg aacaaaagga tgacaaatga accgtcttta cccccacccg attatcgccc
     4621 gtgagggctg gccgattatt ggcggcggtt tggctttgag cctgctggtg tcgatgtgtt
     4681 gcggctggtg gtctttgccg ttttgggtgt ttaccgtatt tgccctgcag tttttccgcg
     4741 accctgcgcg tgagattccg caaaatcctg aagcagtgtt gagtccggtt gacggccgta
     4801 tcgtggtggt cgagcgcgca cgcgatccgt atcgtgatgt cgatgctttg aaaatcagta
     4861 tttttatgaa cgtgttcaac gtgcattcgc aaaaatcgcc tgccgattgt acggtaacga
     4921 aagtggtcta taacaaaggc aaattcgtga atgcggattt ggacaaagcc agcacggaaa
     4981 acgaacgtaa tgcggtgttg gcgactacgg cttccggtcg tgaaattact tttgttcaag
     5041 tggccggttt ggtggcgcgc cgtattttgt gctacaccca agcaggtgcg aaactgtccc
     5101 gcggcgaacg ttatggcttt atccgcttcg gttcgcgcgt ggatatgtat ctgcctgtcg
     5161 atgcgcaggc gcaagtggcg attggcgata aagtaactgg cgttagcact gtattggcgc
     5221 gtttgccgct gactgcgccg caaatcgaat ccgagcctga gtctgagcct gctttacaaa
     5281 ctgctccggt tgaaacagcg gcaaacccat ctgccgaaca acggcaaatc gaggcagcgg
     5341 cggctaagat tcaggcggct gtgcaagatg tgttgaaaga ttaattttgc gaactgaaat
     5401 agaaaatatc agtaccatca ttcacacgaa tgaggaagtt tggttttttg aatttttgct
     5461 aatgttcaca ccgttatgtt cacgaaaatg ggaatctgga aatttaacgt tacggtgatt
     5521 tatca
//
LOCUS       DAWKST010000133         5498 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.133, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000133 DAWKST010000000
VERSION     DAWKST010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.133"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..151)
                     /locus_tag="WJL87_001632"
     CDS             complement(<1..151)
                     /locus_tag="WJL87_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEZ7800043.1"
                     /translation="MLKTLTFAALHFSTAFSITYVLTGSIGVSGAVALVEPLINTVVF
                     YSGLTK"
     gene            273..1226
                     /locus_tag="WJL87_001633"
     CDS             273..1226
                     /locus_tag="WJL87_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ7800044.1"
                     /translation="MNQKKVVVLDADTLPGRTFHFDFPHELAVYGTTDAAETAGRVRD
                     AHIIITNKVMISADIIAHAPQLELIAVSATGVNNVDIGAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHAPDVREGYVSFEDAVQTADVLSLHCPLNAQTENMIGEN
                     ELRQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTEEPPRGGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(1304..3358)
                     /gene="metG"
                     /locus_tag="WJL87_001634"
     CDS             complement(1304..3358)
                     /gene="metG"
                     /locus_tag="WJL87_001634"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ7800045.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECY
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTDFGIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQAE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFESRLKDVADSALLAKLTAQSEAIAECYESREYAKALRD
                     IMALADIVNEYVDANKPWELAKQEGQDARLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AARFLNLDAITWANTRVTLGEHAINKYEHLMQRVEQKQVDDLIEANKQSIQTTPAPVE
                     DSKYEKVAEQAGFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIAA
                     FYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGDK
                     VG"
     gene            complement(3464..5302)
                     /gene="glmS"
                     /locus_tag="WJL87_001635"
     CDS             complement(3464..5302)
                     /gene="glmS"
                     /locus_tag="WJL87_001635"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ7800046.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGNIK
                     RVRRVGRVQLMEDAAREKHLNGSLGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEDLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSEEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGKLSPVVHTIPVQLLSYHVALARGTDVDKPRNLAKSVTVE"
BASE COUNT         1117 a         1332 c         1674 g         1375 t
ORIGIN      
        1 tttttgttaa tccactatag aaaacgacgg tgttgattag aggctcgacc agcgcgaccg
       61 cccccgatac gcctatactg cccgtcagta cataggttat actgaaggcg gtgctgaaat
      121 gcagtgcggc aaaagtcagg gttttaagca tcatcatctc tcggattgga cattgacgga
      181 gggatgataa agattatcat aaggctgtgc ggtttaaatt tgctatttgc tgttagtgta
      241 aataaatcat tttctaaata gggataaaaa ttatgaatca gaagaaagta gtcgtattgg
      301 atgcggatac tttacccggt cggacttttc acttcgattt cccacacgag cttgcggttt
      361 acggtacgac agatgctgcg gaaacggcag gacgggtgcg cgacgcgcat attatcatca
      421 ccaataaagt gatgatttca gcggatatca ttgcacacgc tccgcaactt gaattgattg
      481 ccgtcagtgc gaccggtgtg aacaatgtcg atattggggc ggcgaaggcg gcgggcgttg
      541 cggtatgcaa tgtccgcgcc tacggaaacg aatcggttgc ggaacacgcc tttatgctga
      601 tgattgcgtt gatgcggaat ttgcctgcct atcagcgtga tgttgcggca ggattatggg
      661 agaagtcgcc gtttttctgc cattacggcg cgccgattcg ggatttgaac ggtaaaacgc
      721 tggcggtttt cggacgtggc aatatcggac ggacgcttgc cggatacgcg caggcattcg
      781 gtatgggggt ggtgtttgcc gaacacaaac acgcgcccga cgtgcgcgaa ggctatgttt
      841 cctttgaaga tgcggtacag actgccgacg tgttgtcgct gcactgtccg ctaaacgccc
      901 aaactgaaaa tatgataggc gaaaacgaat tgcggcagat gaagcctggc gcggttttaa
      961 tcaattgtgg gcgcggcggg ctggtggatg aaaacgcgct gcttgccgca ctcaaatacg
     1021 ggcagatcgg tggggcgggt gtcgatgttt tgacggagga gccgccaagg ggcggcaatc
     1081 ccttgctgaa tgcacgcctg cccaatctga ttgttacgcc gcataccgcg tgggcgagcc
     1141 gcgaggcttt ggacaggctg tttgatatat tgttggcgaa cattcacgcc tttgtgaaag
     1201 gcgaggcaca aaatcgggtg gtttgagctt gtcgggatta tgggaaaaca atgccgtctg
     1261 aaggtctcaa gggtccagac ggcattgttt ttgatatttc tacttaaccg actttgtcgc
     1321 ccggttgcgc gcctgcgtcg acatcaagca gtttcagttt cccgtctgcc gtggcggcac
     1381 tcaaaatcat gccttcggat acaccgaatt tcgccatttt gcgcggggcg aagttggcga
     1441 cggcaatgac cattcttccg tttaactcgg cagggttggg gtaaaacgcg gcgatgccgg
     1501 agaagatgat acgtttttca aaaccgaaat cgaggtcgaa tttcaggagt ttggtgctgc
     1561 cttcgacggc ttcgcagttc aatactttgg cgacgcgcat atcgattttc ataaagtcgt
     1621 cgaaacccgc ctgttcggcg actttttcgt atttgctgtc ttcaacaggc gcgggggttg
     1681 tctgaatact ttgtttgttg gcttcgatta aatcgtccac ttgtttttgc tccactcgtt
     1741 gcattaaatg ttcgtatttg ttgatggcgt gttcgcccaa ggttaccctt gtgttcgccc
     1801 aagtaatcgc gtccagattg aggaaacgcg cggcgttggc ggcggtttgc ggcaacacgg
     1861 gggcgaggta ggcggtcaac atggtgaagg cgttgatgag ctcgctgcat acttcgtgca
     1921 ggcgtgcgtc ttggccttct tgtttcgcca gttcccaagg tttgttggca tcaacatatt
     1981 cgttgacaat gtctgccaat gccatgatgt cgcgcagggc tttggcgtat tcgcggcttt
     2041 cgtagcattc ggcaatggct tcgctttgcg cagtcagttt tgccagcaac gcgctgtcgg
     2101 caacgtcttt cagacggctt tcaaagcgtt tggcgataaa acctgacgcg cgggcggcga
     2161 tgttgacgta tttgcctacg aggtcgctgt tgacgcgcga gataaagtct tgcaggttca
     2221 aatcgatgtc ttcgattttg ctgttgagtt tggcggcgat gtagtagcgc atccactcgg
     2281 ggtttaaacc ttgttccaga taggatttag cggtgatgaa ggtgccgcgc gatttggaca
     2341 ttttttgtcc gtccacggtc aaaaagccgt gtgcgtacac gccggtcggg gcgcggtggc
     2401 cggagaagtg cagcatggca ggccagaaca gggcgtggaa atagaggatg tctttgccga
     2461 tgaagtggta catctcggtt tggctgtcgg ctttgaagta ttcgtcaaaa tcgacgccga
     2521 tgcggtcgca caggttttta aacgacgcca tgtagccgac gggcgcgtcc agccagacgt
     2581 agaagtattt gcccggcgcg tcggggattt caaagccgaa atacggcgcg tcgcgggaaa
     2641 tgtcccagtc ggacagcgtg gtttcttcgc cttcgcccag ccattctttc attttgttga
     2701 gggcttcggc ttgcagatgg ggcttgccgt cgtgcgggtt gttgccggaa gtccatgctt
     2761 tgaggaagtc ggcgcattcg cccagtttga agaagaagtg ttcggattcg cgcaattcgg
     2821 gtttcgcgcc ggaaacggcg gaatacgggt tgatcagttc ggtcggggaa taggtcgtgc
     2881 cgcagacttc gcagttgtcg ccgtattggt cttgggcgtg gcatttcggg cattcgcctt
     2941 tgacgaagcg gtcgggcagg aacatttgtt tttcggggtc gaaaagctgc tcgatgacgc
     3001 ggctctcaat cttgccgttg gctttcagcg cgcggtaaat gtcttgggaa aactgtttgt
     3061 tttcagggga atgggtgctg taataattgt cgtaaccgat gccgaaatcg gtaaagtcgg
     3121 cgaggtgctc ttcgcgcact ttggcaatca tgtcttcggg cgcgatgcct tgtttttgcg
     3181 cggcaagcat tacgggcgtg ccgtgagtgt cgtcggcgca gcagtagtag cattcgtggc
     3241 cgcgcagttt ttgaaagcgc acccaaacgt cggtttggat gtgttcgacc atatggccga
     3301 ggtggatgct gccgttggca tagggcaggg cggaggtaac gaggattttg cgtgtcatgg
     3361 tttatgcttt tggggataga tggtaaaggg gaattatacc gtaaatgccg tctgaagcct
     3421 gaaaaatcag gcttcagacg gcatgttcgc aaacaatccg gacttattcg accgtaacgg
     3481 atttcgccag gttgcgcggt ttgtctacat cggtgccgcg tgcgagggcg acatggtagg
     3541 aaaggagctg cacggggata gtatgcacga cgggggacag tttgccgacg tggcgcggtg
     3601 cgcggataac gtgcacacct tcggtggcat taaaattgct gtcgaggtcg gcaaagacga
     3661 aaagttcgcc gccgcgcgcg ccgacttcct gcatattggc tttgactttg tccaacaggc
     3721 tgtcgttggg tgcgatgacg acgacgggca tgttttcgtc caccaaggca agcggcccgt
     3781 gcttcagttc gccggcagga taggcttcgg cgtggatgta ggtgatttcc ttcagtttta
     3841 acgcgccttc gagggcaatc gggtaatgga tgccgcgccc caaaaacagc gcgctggttt
     3901 tcttggcaaa ctgttgcgcc caggcggcaa tttgcggttc gaggtttaaa gcgtgctgca
     3961 cgctgccggg aagctggcgg agttcttcgg tgtaacgtgc ttcgtcttct tcggaaacca
     4021 aaccgcgcac tttcgccagc gttaccgcca aaccgaacag cgcaaccagc tgcgtggtaa
     4081 atgctttggt tgaggcgacg ccgatttccg caccggcacg ggtataaagc acgaggctgc
     4141 tttcgcgcgg cagggcggat tccatcacgt tgcagatgga gaggctgtgg cggtgtccca
     4201 aggattttgc gtatttcaac gcctccatcg tgtccagcgt ttcgccggat tgggaaatgg
     4261 taatgaccag ttggtccgga tcggcaatca cgctgcggta tcggtattcg ctggcgattt
     4321 cgacatcggc cgggattttg gcgatggatt ccaaccaata tttggcggtc agcgcggcgt
     4381 aataggacgt gccgcaggca aggattttga cgctgcggat gctttcaaac acgcttttgg
     4441 cgtttttgcc gaagttttcg gggatgaagc cgccgtcgag gaaaacctcc gccgtgtctg
     4501 caatcgcgcg cggctgctca tggatttcct tttgcataaa gtggctgtac ggtcccagtt
     4561 ccaaagaggc gagcgagagt tcggatacct tgactttgcg ttcggcaggc aggccgtttt
     4621 tatcggtcag ccttttgatg ccgtctgaag ccagcagcgc gatgtcgccg tcttcgaggt
     4681 acgccacgcg gcgcgtaaag gcgatgacgg cggatacgtc cgaagcgata aaggtttcat
     4741 cgtcgcccaa agcgaccaaa agcgggcagc ccatacgcgc cacgactaat tcatcaggct
     4801 tgtcttgggc aataaccgcg atggcgtatg cgccgtggaa acgtttgacc gcttcctgca
     4861 cggcttcaaa cagcctgccg ccgttttgcg cgtattcgtg attgatgctg tgggcgatga
     4921 cttcggtatc cgtttgcgat tcaaaacggt atcccaaatc ttccaaacgt ttgcgttcgc
     4981 tttcaaagtt ttcgatgatg ccgttgtgca cgaccgcaat cataccgccg ctgatgtgcg
     5041 ggtgggcgtt cggctcggta acgccgccgt gtgtcgccca gcgcgtatgc ccgatgccca
     5101 aactgccgtt caaatgcttt tcacgcgccg catcttccat taactgcacg cgcccgacac
     5161 ggcgcacgcg cttgatgttg ccgtcggtgt tgacggcaat gcctgatgaa tcataacccc
     5221 ggtattcgag gcgtttgaga ccgtcggtca gaaaatcgac gacattgtga tgggcgcgga
     5281 tggcgccgac gataccgcac ataactgttc cttagtatcc ggttgaaaaa aaaacaggcg
     5341 cggacggctc ccgtgccgca ccttccgctt cggattataa accgcctccc gcgccggaaa
     5401 acagcaaaat gccgtctgaa ggcttgggct tgctcaaaaa agggagggat ttccccgcct
     5461 gaccgggatg ggcgttcaga cggcattgcc tgctgccg
//
LOCUS       DAWKST010000134         5450 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.134, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000134 DAWKST010000000
VERSION     DAWKST010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.134"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            10..789
                     /gene="rsmA"
                     /locus_tag="WJL87_001636"
     CDS             10..789
                     /gene="rsmA"
                     /locus_tag="WJL87_001636"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEZ7800047.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            962..1690
                     /locus_tag="WJL87_001637"
     CDS             962..1690
                     /locus_tag="WJL87_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ7800048.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            1763..2122
                     /locus_tag="WJL87_001638"
     CDS             1763..2122
                     /locus_tag="WJL87_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800049.1"
                     /translation="MYFVDRTAVVLKPTARFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDINKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEAELNITPVFDNMM"
     gene            2127..2567
                     /locus_tag="WJL87_001639"
     CDS             2127..2567
                     /locus_tag="WJL87_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ7800050.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASLAVCLTWFYGRMMWFGLAA
                     LVASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKDKTAATLAGSSMVTPYALFLQ
                     WDTGGKTVSQCIIPDMTDKESYRRLKVWVLWRQPKKTAETDTPS"
     gene            2599..3873
                     /gene="folC"
                     /locus_tag="WJL87_001640"
     CDS             2599..3873
                     /gene="folC"
                     /locus_tag="WJL87_001640"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ7800051.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGFKVGTLTSPHLLRYNERIAVNAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNALAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPASLVAHAEAIGAKLLMVQRDF
                     EFHALENIQWNYRFHPQHSDDPTRNRNALPFPALRGAYQLSNAACTLTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHGIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            3886..4884
                     /gene="ftsN"
                     /locus_tag="WJL87_001641"
     CDS             3886..4884
                     /gene="ftsN"
                     /locus_tag="WJL87_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ7800052.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETNGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKETKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            4877..5374
                     /locus_tag="WJL87_001642"
     CDS             4877..5374
                     /locus_tag="WJL87_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ7800053.1"
                     /translation="MNNLPVADLLVFAIIAACIVLSAMRGVIAEAGSMAAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGILIITLLVMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHSGGTAE
                     TPEDD"
BASE COUNT         1441 a         1530 c         1408 g         1071 t
ORIGIN      
        1 ggaaacacga tgaaagaaca caaagcccgc aagcgtttcg ggcagaattt tttgcaggac
       61 acgcggatta tcagcgatat tgtcaacgcc gtgcgcccgc aggcggatga tgtcgtgatt
      121 gaaatcggtc cgggtttggc ggcgattacc gaacctttgg cgaaaaagct gaaccgcctg
      181 cacgttgtcg aaatcgaccg cgacatcgta tgccgtctga aaacgctgcc gtttgcggat
      241 aaactggtga ttcacgaagg cgatgtattg cagtttgatt tcaacggcat cgcaggcaaa
      301 aagaaaatcg tcggcaacct cccgtacaac atttccacac cgcttttgtt caagctggcg
      361 gaggtggcgg acgatgtcgt cgatatgcac tttatgctgc aaaaagaagt ggtcgagcgt
      421 atggttgccg cgccgaaaag caacgactac ggccgcttgg gcgtgatgct gcaatatttt
      481 ttcgatatgg aaatgctgat tgacgtaccg cccgaatcgt tcgaccctgc gccgaaagtg
      541 gattccgccg tcgtccgtat gattccggtg aaacaccgca tcggcaaggc ggacgatttc
      601 gagcatttcg ccaaactggt gaaactcgcc ttccaccaac gccgcaaaac catacgcaac
      661 aacctgaaag agcttgcagg cgacgatgat ttgcaggctg tcggcatcaa tccgcaagac
      721 cgtgccgaac acatcgcgcc ggaaaaatac gtagcgttga gcaattatct ggcgggcaag
      781 gccgtctgaa aagggagtaa gccgatgaag aaatacctgc ttttacccct gatactgccg
      841 cttgcagcct gcctgaccaa tccgaacgac cgtttttttg acggcagacg ctacacgctt
      901 catcatatgg agctcccgta acgacaacct gcaacggcaa atgccgtctg aaagaaaaga
      961 catgatcaaa ttcaaaaacg tacacaaaca tttcaaagac ctgcacgtca tcaacggcgt
     1021 gaacttggaa atcaaaaaag gcgaagtggt cgtcgtctgc gggccttcgg gcagcggcaa
     1081 atccacactc atccgcaccg tcaaccagct tgaaagcatc gaaagcggcg agatttgggt
     1141 ggacggtgtc aacgttgccg acccaaaaac cgatttgaac aaaatccgcg aagaagtcgg
     1201 ctttgtgttt caaagtttca acctctaccc gcacctgacc gtgttggaaa acattaccct
     1261 cgcaccgatg aaggttaagg ggcaaaatgc ggaacaggcg gagaaaaagg caatggagct
     1321 tttggaacgc gtcggactgg cacacaaaaa agacgccttc ccttcccaac tttccggcgg
     1381 ccagcagcaa cgcgtggcga ttgcacgcgg tttggcgatg gaaccgcgcg taatgttgtt
     1441 tgacgaaccg acctccgcac tcgaccccga aatggtcggc gaagtgttga aagtgatgaa
     1501 agacttggca gaaagcggca tgaccatgat gtgcgtaacc cacgaaatgg ggtttgcccg
     1561 cgaagttgcc gaccgcgtga ttttcgtcga taaagggcaa atcctcgaag acgaaacgcc
     1621 ggaagcgttt tttaccaacc cgaaacacga acgcgccaag cagttcctgc aacaggtcat
     1681 gacccattga tgctatgccg tctgaaaacc gaacgttcag acagcatatc ccgacaccgc
     1741 aagatgattg agaaagaaaa ccatgtattt tgttgaccgc accgccgtcg tcctcaagcc
     1801 gaccgcgcgc tttttggaat ggctcaaaag caccgacgaa aatatgcccg acctgaccat
     1861 agagcagctt cgtgccaact gttccgtctt cctcgttccg cagttcgacg aaccggaagc
     1921 cgtcgtttcc tacttcgacg aacgctaccg ccagattttc gaggcggagc tggcaggttg
     1981 ggacatcaat aaggataaat ggccgcaaga tatggggctg aaggcgtttt gggaattttt
     2041 cgacatcgaa atccacgata tggtgttgga catggaagaa gcggaattga acattacccc
     2101 cgtgttcgac aacatgatgt aaggctgtgc gcgcctttca gacggcattg aggccgtccc
     2161 gcatcctgaa catcctgacc gtatcgctcc actcggcttc ccttgccgtc tgcctgacct
     2221 ggttttacgg ccggatgatg tggttcggtt tggcggcttt ggtggcaagt tacgcatact
     2281 cgctcaggat aacgaatctg aaacaccgcc atgccataac cgccattacc atagaccgcg
     2341 acggacaggc ggaaatcgta tccggcaaag acaaaacagc ggcaacactt gcgggcagca
     2401 gtatggttac gccttatgcc ttattcttac aatgggacac cggcggcaaa accgtcagcc
     2461 aatgcatcat acccgacatg acggacaaag aatcctaccg gcggctgaaa gtctgggtgc
     2521 tttggcggca gccgaaaaaa accgccgaaa ccgatacacc aagctgattt cagacggcat
     2581 ttgaacacgg aacactcaat gaaaacactg caagactggc tctcccattt ggaaaccgcc
     2641 cacagcggtg gcttgatcga tatgggcttg gaacgcgtag gcgaagtgaa aaaacggatg
     2701 aagctcacgc cgcaatgccc cgtcgtcgtt gtcgcgggaa caaacggcaa aggttcggtc
     2761 tgcgcctacc tgacacagat ttacaaacag gcaggcttta aagtcggtac gctgaccagc
     2821 ccgcatttat tgcgttacaa cgaacgcatc gccgtcaacg ccgaacccgt ttcggacgat
     2881 acgattattg cctctttcga gcgcatcgaa gcggcgcgcg gcgaaatatc gctgacttat
     2941 tttgaattca atgccttggc ggccgtcgac atcttcatgc gcgaacaggt tgacgtgatg
     3001 atattggaag tcggcttggg cggacgcttg gacgcggtca acgcatttga cggcgattgc
     3061 gcggtggtta ccagcgtgga tttggaccat caggcgtttt tgggcgatac ggtcgagcag
     3121 gtcggctttg aaaaagcagg cgtgttccgc agcggcaaac ccgccatctg cggtcaaaac
     3181 cccgcgcccg catcattggt cgcacacgcc gaagccatag gcgcgaaact gctgatggtg
     3241 cagcgcgatt tcgagtttca cgcgctggaa aacatccaat ggaactaccg cttccatccg
     3301 caacattcag acgaccccac gcgcaaccgc aatgccctgc ccttccccgc attgcgtggc
     3361 gcataccagc tttccaacgc cgcctgcacg ttgaccgtat tggaatgctt gaacgacaaa
     3421 ttgcctgtgg acatcggcgc catcaaacgc ggattgctgc tggttgaaaa tcccggccgc
     3481 ttccaagtcc tgcccggccg tccgctgacc gttttggatg tcggacacaa tccccacgct
     3541 gcccgcgccc tgcgccgcag cctgattaat ttggcgtttg cgcaaaaacg caccgccgta
     3601 ttcagtatgc tgtccgacaa agacatagac ggcgtgttgg aaatcgttaa agaccagttt
     3661 gacgagtggt atatcgcacc gttggatgta ccgcgcggca tgacgctcga gagcctgcaa
     3721 aacaaactgc acgaacacgg catcgaaaac atacagacat tcgccgccgt ccgcgaagca
     3781 taccgggcgg cagcctcaaa agcgggcgag aacgacagaa tcgtcgtttt cggctcattt
     3841 cataccgttg ccgatgtcat gtcggtactt taaaaggaaa cttctatgtc cgaaaacaaa
     3901 caaaacgaag tcctgagcgg ttacgaacaa ctcaaacggc gcaaccgccg ccgcctcgta
     3961 acggcaagtt gcctggttgc cgcctcctgc atcctgctgg cagccgccct cagttccggc
     4021 cctgccgaac agactgccgg cgaaacaaac ggcgtagaaa acaaagcggc aggtgcggca
     4081 caaacccctg ccttgaaatc cgccgccgac aaaccgcagg acttggcagg cgaagacaag
     4141 ccttctgccg ccgacagcga aatcagcgag cctgaaaacg taggcgcgcc gctggtgctg
     4201 attaacgacc gcctcgaaga cagcaacatc aaaggtttgg aagcatccga gaaactgcaa
     4261 caggcagaaa ccgccaaaac cgcaccgaag caggcaaaac aacgcgctgc cgaaaaagtg
     4321 ccggcaactg ccgacagtac ggatacggta gcggttgaaa aaccgaaacg cactgccgaa
     4381 acaaaaccgc aaaaagcgga acgcactgcc aaagccaagc ccaaagccaa agaaaccaaa
     4441 accgccgaaa aagttgccga caaaccgaaa actgccgccg aaaaaaccaa accggatacg
     4501 gcaaaatccg acagcgcggt aaaagaaacg aaaaaagccg acaaggctga aagcaaaaaa
     4561 acagccgaaa aagaccgttc ggacggcaaa aaacacgaaa cggcacaaaa aaccgacaaa
     4621 gcggacaaga ccaaaaccgc cgagaaggaa aaatccggta aaaaagccgc cattcaggca
     4681 ggttatgccg aaaaagaacg cgccttaagc ctccagcgca aaatgaaagc ggcgggtatc
     4741 gattcgacca tcaccgaaat tatgaccgac aacggcaaag tttaccgcgt caaatcaagc
     4801 aactataaaa acgcaaggga tgccgaacgc gatttgaaca aattgcgcgt acacggtatc
     4861 gccggtcagg taacgaatga ataacctccc cgttgccgac ctcctcgtct tcgccatcat
     4921 cgccgcctgc atcgtgctat ccgcgatgcg cggcgtgatt gcggaggcag gttcgatggc
     4981 ggcatgggtg gtttccttct tttttgccaa actctttgcc gcacccttcg ccgacctcgc
     5041 ctttgcctcg ttccaacccc gcctgtttgc attggctctg tcgttcattt ccctgttcgt
     5101 cattgcctgt ctgatccaga aaatgctccg ttcgctgctg accggcgcag tttcggcggt
     5161 cggtttgggc tttgccaacc gcattttggg cggcgtattc ggtgcattga aaggcatttt
     5221 gattattacc ctgctggtca tgctcgcttc aaaaaccgac ctgcccgata ccgaagaatg
     5281 gcggcaatct tacacactgc cgtttttcgt atcgctttcc gaagccgtgt tgaaccatag
     5341 cggcggcacg gcggaaactc cggaagacga ttgattgccc tgctggcgtt tcagacggca
     5401 ttctctttta tgccgtctga aatgtttttt cctttcctca gccatatcac
//
LOCUS       DAWKST010000135         5401 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.135, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000135 DAWKST010000000
VERSION     DAWKST010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.135"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(90..1886)
                     /locus_tag="WJL87_001643"
     CDS             complement(90..1886)
                     /locus_tag="WJL87_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEZ7800054.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVSTFVVTADEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLAAKNIRIEHPNDLLDQVWTSRPAIPAETVFIH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDNAVLFTEQCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVCLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFVSLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLIDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2344..3597
                     /locus_tag="WJL87_001644"
     CDS             2344..3597
                     /locus_tag="WJL87_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ7800055.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3752..5263
                     /gene="lysS"
                     /locus_tag="WJL87_001645"
     CDS             3752..5263
                     /gene="lysS"
                     /locus_tag="WJL87_001645"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ7800056.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSDIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIIDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
BASE COUNT         1173 a         1372 c         1590 g         1266 t
ORIGIN      
        1 cttgcggcaa gatgaaaacg gtgcgggatg ttttgggaaa ccaatgccgt ctgaagggct
       61 ttcagacggc attttttgcg ccgtgctgtt taacgcgcca gcggttcggt gcgtttgatc
      121 agccacgctt ttgccgcgcc ttcggtcagc ggctcgaggc ggcggcggac ttcggcgtgg
      181 tagcggttga cccagtcgat ttcgccgtcg gtcatgaggg cggtgtctat caggcgggtg
      241 tcgatggggc agagggtcag ggtttcaaaa cagaggaagc tgccgaattc ggtttcttga
      301 ggggcggcga cggcttggtt ggcggcaagg ttttcaatgc ggatgcccca ttttcccggg
      361 cggtagagtc cgggttcgat ggaggtaacc atgccttttt tcatggcggt ttcgggcgtg
      421 gcgggggcgg cgaaggcgat gcgctgcggg ccttcgtgga cgttgaggaa atagcctacg
      481 ccgtgtccgg tgccgtggcc gtagtcgcat tgcgcctgcc acaggggttt gcggcaaatc
      541 gcatcaatca gcggcgaggg gatgttttct gggaacacgg cttcggcaag cgcgatatgg
      601 gctttgagaa cgagggtgtt gtcgcgtttt tgttcggcgg tgggtgtgcc gacagggacg
      661 acgcgggtga tgtcggtcgt gccgcctttg tattgcgcgc cggagtcgat gagcagcagc
      721 ccgttgccgc tgatggtgct gtggctttcg ggcgttgcgc tgtaatgcgg cagggcgccg
      781 ttggcgttga agcctgcgat ggtgtcgaaa ctcaatgaca caaaacccgg gcgcacgctg
      841 cggtggcggt aaagcatggt gtccacgtcg atttcggtca ggctgccgcc gttgccgatg
      901 atgtcttcaa actcggcgaa gaaaccgcac aacgccgcgc cgtcgtgttc catcgcttcg
      961 cggatgcggg cgatgtcggc ttcggatttg caggatttga acagcgtgga cgggttgatg
     1021 ccttcgataa ggcacacgct ttcgggcagg cgcacaagcg tgctgacggc ggttttgttc
     1081 ggttcgataa gcagcgcgcc gccgatttgc gcgagtttgt cggcaacttg ggcataaggt
     1141 tcgaccgtga tgcctgcggt ttgcagcgcg gcggcggctt cggcgttcag acggcattgt
     1201 tcggtaaaca ggacggcgtt gtctttgcca atcagcagga aggacacgaa aacagggttg
     1261 aaaggcacgt cgctgccgcg caggttggtc agccaggcga tgtcgtcaag cgaggaaacc
     1321 aagtggtaat ccgcgccttt ttccgccatc acggcgcgca cgcgggcgag tttttcggcg
     1381 gcggtttcgg agacgtaggc ggggtcgtgg atgaacacgg tttcggcagg aatggccggg
     1441 cggcttgtcc acacttggtc aagcagatcg tttgggtgtt cgatgcggat gtttttggcg
     1501 gcgagcgatt gcgccaaagt gcgtttgccg gtgagcgaga ccatatcgga agggatgccg
     1561 acggcggcgt tttcgggcag gtttgccgcg agccattcgt tatacggcgg cacttgcccg
     1621 cttttttgca gttcgatgcc gctgcccgca agctgcttgg cggcttgttc ccaatagcgg
     1681 ctgtccaccc atacgcccgc ttcgtcggcg gtaacgacga atgtgctgac cgagccggta
     1741 aagcccgata attcgcggcg cgcctgccaa tgctcgggca ggtattcgga caggtggggg
     1801 tcggcggaag ggatgacgag tgcatccaag ccttgcgcct tcatggcttc gcgcaatgcg
     1861 gacaggtaat tcgatacggt attcataaca gttcctccaa tcgggttttg cggcttcaga
     1921 cggcatggac ggaaatctga aatgccgtct gaaaaacata accatcttat caaatcgcca
     1981 gcctgctgca aaacggacgg gctaatcgga tggtgcgtgt ttgcgcgaaa aaagcgatgt
     2041 aaggttgttt gttgtaaaat tttaggcata aaatgtcgtt tcatgccttt ttttggaagt
     2101 aatacggaaa atatagtcaa gtttgagcat gaagttctaa tggtgtgtgt tggcaggtat
     2161 ttggattacg ttacaatacg tgttttcatc aaaatttccc taccgagcgg tatcctgttt
     2221 ttgaagacgg taggatttgg gtttgtttgg gggcaatccc gcccaaattg gagcaataga
     2281 tagggctgtg tccgaatatg cggctctgtg tgttgaaaca cggttaaaaa tacggaagac
     2341 tttatgtccg aacaacatat ttcgacttgg aaaagtaaaa tcaacgcact ggggccgggg
     2401 attatgatgg cttcggcggc ggtcggcggt tcgcacctga ttgcctcgac gcaggcgggt
     2461 gcgctttacg gctggcagat cgcgctcatc atcatcctga ccaacctctt caaatacccg
     2521 tttttccgct tcagcgcgca ttacacgctg gacacgggca agagcctgat tgaaggttat
     2581 gccgagaaaa gccgcgttta tttgtgggta ttcctgattt tgtgcatcct ctccgccacg
     2641 attaacgcgg gcgcggtcgc cattgtaacc gccgccatcg tcaaaatggc gattccctcg
     2701 ctgatgtttg atgccggcac ggttgccgcc ttgattatgg catcctgcct gattattttg
     2761 gtgagcggac gttaccgcgc tttggatcgc gtttccaaaa tcatcatcgt tactttgagt
     2821 atcgccacgc ttgccgccgc cggcatcgct atgtcgcgcg gtatgcagat gcagcccgat
     2881 tttatcgagc cgacaccgtg gacgcttgcc ggtttgggct tcctgatcgc gctgatgggc
     2941 tggatgcccg cgccgattga aatttccgcc atcaattctt tgtgggtaac cgaaaaacaa
     3001 cgcatcaatc cttccgaata ccgcgacggg atttttgatt tcaacgtcgg ttatatcgcc
     3061 agtgcggttt tggctttggt tttccttgca ctgggtgcgt ttgtgcaata cggcaatggc
     3121 gaagcagtgc agatggcggg cggcaaatat atcgggcaat tgatcaatat gtacgccgtt
     3181 accatcggcg gctggtcgcg cccgctggtg gcgtttatcg cgtttgcctg tatgtacggc
     3241 acgacgatta ccgttgtgga cggctatgcc cgtgccattg ccgaacccgt gcgcctgctg
     3301 cgcggaaaag acaaaacggg caacgccgaa ttctttgcct ggaatatttg ggtggcgggc
     3361 agcggtttgg cggtgatttt ttggtttgac ggcgtaatgg cgaatctgct caaatttgcg
     3421 atgattgccg cttttgtgtc cgcccctgtg tttgcctggc tgaattaccg tttggtcaaa
     3481 ggtgatgaaa aacacaaact cacatcaggt atgaatgccc ttgcattggc aggcttgatt
     3541 tatctgaccg gttttaccgt tttgttctta ttgaatttgg cgggaatgtt caaatgataa
     3601 cgatgccgtc tgaaaccgca aaccgctttc agacggcatt gtcgcgttta tggaaaaaaa
     3661 tgccgacatc gcgtaaaata tgcgcaaatt ttgtgattcg gtcaggtcgt ctgaaacaga
     3721 ttgcgcctga tttatttttt cggaaaaccc tatgagcgaa caaaaccatc cgcaaaccga
     3781 gccgcagttg gacgaaaacc aaatcatcgc cctgcgccgc gaaaaactgc acaacatccg
     3841 ccaacagcgc aacgcctatc ccaacgactt caaacgcgac agcttcgccg ccgatttgca
     3901 cgcccaatac ggcgaaatcg gcaaagaaga actcgatccg caaggcattc ccgtcaaagt
     3961 ggccggccgc atgatgctga agcgtcaaat gggcaaggcg agctttgcca ccattcaaga
     4021 cgtgtccggg caaatccagc tttatctgaa caacaaaggc gtgagccaag aagttttgga
     4081 cgacttcaac cattgggatt tgggcgacat cgtcggcgcg gaaggcactt tgttcaaaac
     4141 caaccacggc gaactgaccg tacgcgtgtc cgacatccgc ctgttgtcca aatccttgcg
     4201 tccgctgccg gacaaacaca aaggcttgag cgatcaggaa accaaatacc gccaacgcta
     4261 tgtcgatttg attgccaacg aagaatcgcg caataccttt atcaaacgca gccaaatcat
     4321 ccaatccgtg cgtaatttta tggtgggcga gcattatctc gaagtcgaaa ccccgatgat
     4381 gcacccgatt cccggcggcg cgacggcaaa acccttcgtt actcatcaca atgccttaga
     4441 tattccgctt tacctgcgta tcgcgcctga gctgtatttg aaacgcctgg ttgtcggcgg
     4501 cttggaacgc gtgtttgaaa tcaaccgcag cttccgcaac gaaggcatgt ccgtgcgcca
     4561 caaccccgaa tttacgatga ttgaatttta cgaagccttc tccgactacg aacgtatgat
     4621 gcagatggcg gaagacatca tccgcaacgc atcgcgcaca gtaaacggta cggcaaacat
     4681 cacttacaat ggcaaagaag tcgatttgga aagcccgttc gagcgtctga ccatcctcga
     4741 agccatcaaa aaatacaatc cgcactacac cgacgagcag ttgaacgatg cggaatggct
     4801 gaaaaaagaa atcgtcaaac acggtgaaag cctgccgccg tccccgggca tcggcagcct
     4861 gcaactcgcg ctgtttgaag gttgcgccga gggcaagctg tggaacccga ctttcatcat
     4921 cgattacccg gtcgaagttt ccccgttggc gcgcgcttcg gataccaaac aaggtctgac
     4981 cgaacgtttc gaattgttcg ttgtcggccg cgaactggca aacggctatt ccgagttgaa
     5041 cgaccccgaa gaccaagccg aacgcttcaa agcgcaagtg gcgcaaaaag acgcgggcga
     5101 cgacgaagcg atgcactacg atgccgacta catccgcgcg atggagttcg gtctgccgcc
     5161 gactggcggt tgcggtatcg gcatcgaccg cttggtaatg ttgctgaccg attcgcaaac
     5221 catccgtgac gtgattctgt tcccgcagat gcgccccgag taatcataaa aacagttgaa
     5281 atggcaatgc cgtctgaacc cgattggatt cagacggcat tttgtatggc aggattgata
     5341 gtgaattaac aaaaatcagg acaaggcggc gagccgcaga cggtacaaat agtacggaac
     5401 c
//
LOCUS       DAWKST010000136         5392 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.136, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000136 DAWKST010000000
VERSION     DAWKST010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.136"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            82..1047
                     /locus_tag="WJL87_001646"
     CDS             82..1047
                     /locus_tag="WJL87_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7800057.1"
                     /translation="MLRLTALTLCTVLALGACSPKDSDSAPQAKEQAVSAAQSEGASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKKAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGVPAVDESIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLNNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            1065..1214
                     /locus_tag="WJL87_001647"
     CDS             1065..1214
                     /locus_tag="WJL87_001647"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800058.1"
                     /translation="MPSETRMQTASCVSDGIARYGGFKQGFPLRRFGLPCLNLLRLNA
                     FPFCL"
     gene            1211..2179
                     /locus_tag="WJL87_001648"
     CDS             1211..2179
                     /locus_tag="WJL87_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ7800059.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFVAY
                     EFEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFIGLVVPNIISRLIGDRLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            2169..3143
                     /locus_tag="WJL87_001649"
     CDS             2169..3143
                     /locus_tag="WJL87_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ7800060.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCVLFMTLNVKGDWDFVL
                     QLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYASLPLTGKFGFELVVMMGGSLLLFYTLIKQGGRDLSRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAVVWRERYRLDVYLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVKHSVRLP
                     MTVCIGGILLVGGQTVFEHLLGMQAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            3187..3813
                     /locus_tag="WJL87_001650"
     CDS             3187..3813
                     /locus_tag="WJL87_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800061.1"
                     /translation="MTQEHFPSFFNQAPTITVQDALAEFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAFMQGARDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRHLLSFGVGEINGTLTLRRKDNGKTVAVSLNAALQPFAPE
                     MRELMPKAVGGSASADELKQFGELWQERVRAFLIDQADNPEFVTVSEI"
     gene            3893..4651
                     /locus_tag="WJL87_001651"
     CDS             3893..4651
                     /locus_tag="WJL87_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7800062.1"
                     /translation="MITIRNVSFHIGTCPILDNISLDIPEGGITALIGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLAGTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTREHKRTTVAVLHDINQAAAYADHVVAMKNGKVALTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
     gene            4947..>5392
                     /locus_tag="WJL87_001652"
     CDS             4947..>5392
                     /locus_tag="WJL87_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ7800063.1"
                     /translation="MKHFPSKVLTTAILATFCSGALAATNDDDVKKAATVAIAAAYNN
                     GQEINGFKAGETIYDIDEDGTITKKDATAADVEADDFKGLGLKKVVTNLTKTVNENKQ
                     NVDAKVKAAESEIEKLTTKLADTDAALDATTNALNKLGENITTFAE"
BASE COUNT         1236 a         1530 c         1384 g         1242 t
ORIGIN      
        1 ttatcattta tcctttctag agccgttccg gtttgtccga ccggcggctt tgccccaata
       61 tccccatttt ggagacaccc tatgttgcgt ttgaccgcat tgaccctctg caccgtcctc
      121 gccttgggcg cgtgttcgcc gaaagattcc gactctgccc cacaagccaa agaacaggca
      181 gtttccgccg cacaatccga aggcgcgtcc gttaccgtca aaaccgcgcg cggcgatgtt
      241 caaataccgc aaaaccccga acgcatcgcc gtttacgatt tgggtatgct cgacaccttg
      301 agcaaactgg gcgtgaaaac cggtttgtcc gtcgataaaa accgcctgcc gtatttagag
      361 gaatatttca aaacgaccaa acctgccgga actttgttcg agccggatta cgaaacgctc
      421 aacgcttaca aaccgcagct catcatcatc ggcagccgcg cagccaaagc gtttgacaaa
      481 ttgaacgaaa tcgcgccgac catcgaaatg accgccgata ccgccaacct caaagaaagt
      541 gccaaagagc gtatcgacgc gctggcgcaa atcttcggca aaaaggcgga agccgacaag
      601 ctgaaggcgg aaatcgacgc gtcttttgaa gccgcgaaaa ccgccgcgca aggcaaaggc
      661 aaaggtttgg tgattttggt caacggcggc aagatgtccg ccttcggccc gtcttcacgc
      721 ttgggcggct ggctgcacaa agacatcggc gttcccgctg tcgatgaatc aattaaagaa
      781 ggcagccacg gtcagcctat cagctttgaa tacctgaaag agaaaaatcc cgactggctg
      841 tttgtccttg accgcagcgc ggccatcggc gaagaaggtc aggctgcgaa agacgtgttg
      901 aataatccgc tggttgccga aacaaccgcc tggaaaaaag gacaggtcgt gtacctcgtt
      961 cccgaaactt atttggcggc cggtggcgcg caagagctgc tgaatgcaag caaacaggtt
     1021 gcggatgctt ttaacgcggc aaaataatga aacggcggca ttcgatgccg tctgaaacac
     1081 ggatgcaaac cgcctcctgt gtttcagacg gcattgcccg atacggaggc ttcaaacaag
     1141 gctttccgct ccgacggttc ggactgcctt gtttgaatct tctacgcctt aacgcttttc
     1201 ccttctgttt atgactgcca aacctttttc cctcaacctg accaacctcc tgctgctggc
     1261 ggtattgttt gccgtcagcc tgtcggtggg cgttgccgat ttccgctggt cggatgtgtt
     1321 ttcgctgtcc gacagccagc aggttatgtt catcagccgc ctgccgcgca cgtttgcgat
     1381 tgtgctgacg ggcgcgtcga tggcggtggc ggggatgatt atgcagattc tgatgcgcaa
     1441 ccgttttgtc gagccttcta tggcgggcgc gggtcagagt gcggctttgg gtttgcttct
     1501 gatgtccctg ctgctgcctg ccgcgccgct gccggtcaaa atgtcggttg ccgccgttgc
     1561 tgcgctaatc ggaatgctgg tgtttatgct gctgatccgc cgtctgccgc cgacggcgca
     1621 gctgatggtg ccgctggtgg ggattatttt cggcggcgtg gttgaggcgg tggcgacgtt
     1681 tgtcgcgtat gagtttgaga tgctgcaaat gttgggcgtg tggcagcagg gcgatttttc
     1741 gagcgtgctg ctggggcggt acgagctgct ttggattacg ggcggtttgg cggtgtttgc
     1801 ctatctgatt gccgaccggc tgacgatttt ggggctgggc gagacggtca gcgtgaattt
     1861 gggtttgaac cggacggcgg tgttgtggtc gggtttgatt attgtggctt tgattacgtc
     1921 gctggttatc gttacggtcg gcaatattcc gtttatcggg ctggtcgtgc cgaacatcat
     1981 cagccgcctg ataggcgaca ggctgcgcca aagcctgcct gcggtcgctt tgttgggtgc
     2041 gtctttggtt ttattgtgcg acattatcgg acgcgtgatt gtgtttccgt ttgaaattcc
     2101 ggtctccacg gtttttggtg tgttgggtac ggcgttgttt ttatggcttt tattaaggaa
     2161 acctgctcat gccgtctgaa aaaaatatcg gttttatggc aggaagcagc cgtccgttgt
     2221 gggttgcctt tgcgctgttg ctggtttcct gcgtcctgtt tatgacgctc aacgtcaaag
     2281 gcgattggga ctttgtcttg caactgcggc tgaccaaact tgccgcgctg ctgatggtcg
     2341 cctatgcggt cggcgtgtcc acgcaactct tccaaacgct gaccaataat ccgattctga
     2401 ccccttcaat tttgggtttc gattcgctgt atgtgttttt gcagaccttg ctggtgttta
     2461 cgttcggcgg cgtgggctat gcttccctgc cgttgacggg caaattcggc tttgaactgg
     2521 tcgtcatgat gggcggctcg ctgctgctgt tctacacgct catcaaacag ggcggacgcg
     2581 atttgtcgcg catgatttta atcggcgtga ttttcgggat tttgttccgc agcctgtcgt
     2641 cgctgctttc gcgcatgatc gatcccgaag aatttaccgc cgcgcaggcg aatatgtttg
     2701 ccggattcaa taccgtccac agcgagcttt tgggcatagg cgcgctgatt ctgctcgtca
     2761 gcgcggcggt cgtttggcgc gaacgctacc gcttggacgt ttaccttttg gggcgtgacc
     2821 aagccgtcaa tttgggcatc agctacacgc gcaacacctt atggatactg ctttggattg
     2881 ccgcattggt ggcgacggcg accgccgtgg tcggccccgt aagctttttc gggcttctcg
     2941 ccgcctcgct tgccaaccac ttttccccgt cggtcaaaca ttccgtccgc ctgccgatga
     3001 cggtttgtat cggcggcatc ctcttggtcg gcggacagac cgtgttcgaa cacctgctcg
     3061 gtatgcaggc agtgttgagc gtagtagtag aatttgccgg cggactcgtt ttcctctatc
     3121 tcgttttaaa acacaaaaaa tgacggatgc cgtctgaacg gccgccgccc cgaaaggaca
     3181 aaccatatga cacaagaaca tttcccatca ttcttcaacc aagccccgac cattaccgtc
     3241 caagacgcat tggccgaatt cctcggcgcg gccgaaaacg gcatcctcac ttaccgctac
     3301 gccgatgccg tgcgcctgtg cggacattcc tgcccgaccg tcgcgggcgc gtacctgatg
     3361 gttatcaaag gtctgaaagc actttacggc gaagagctgc ccgaacgcgg cggcatcgaa
     3421 gcctttatgc agggcgcgcg cgacgaaggc acggtcggcg taaccgcgtc cgtcgtccaa
     3481 ctcctcaccg gcgcagcccc cgaaaccggc tttggcggca tcggaatgca gggacgcttt
     3541 gcccgccgcc acctcttatc ctttggtgta ggcgaaatca acggcacact gaccctgcgc
     3601 cgcaaagaca acggcaaaac cgtcgccgtc agcctcaacg ccgccctgca accctttgca
     3661 cccgaaatgc gcgaacttat gcccaaagcc gtcggcggca gcgcaagcgc cgacgaactt
     3721 aaacaattcg gcgaactctg gcaagaacgc gtccgtgcct tcctgattga ccaagccgac
     3781 aaccccgaat ttgttaccgt cagcgaaatc taaccataca ggaataaaat tcagacggcc
     3841 tcatcattta aaggccgtct gaaaatccaa ttccccatat tcgaatccgc ctatgattac
     3901 catccgcaac gtcagcttcc acatcggcac ttgccccatt ctggacaaca tcagcctcga
     3961 cattcccgaa ggcggcatta ccgcgctgat cggcccgaac ggcgcaggca agtccaccct
     4021 gttttccttt atggcgcggc tgcgcccgct tgaaagcggc agcatcgcct accgcggcaa
     4081 aaaccttgcc ggcactccca ccgccgaact cgcccgcacg ctctccatcc tcacccaaga
     4141 aaacagcatc atgagccgca tcaccgtgcg cgacctgctg atgttcggcc gttaccccta
     4201 ccatcaaggc agaccgactg ccgaatgccg ccgtatcgtt aacggtgcaa tcgaagaatt
     4261 ccacctgcaa gacctctccg accgctatct gaccgaactc tccggcggcc aacgccaacg
     4321 cgccatgatt gcgatggtgt tctgccaaag caccgactac gtccttttgg acgaaccgct
     4381 gaacaacctc gatatgtatc acgcccgctc gctcatgcag attctgcaaa agctcacgcg
     4441 cgaacacaaa cgcaccaccg tcgccgtatt gcacgacatt aaccaggccg ccgcttacgc
     4501 cgaccacgtc gtcgccatga aaaacggcaa agtcgccctg accggcacgc ccgaagaagt
     4561 gttcaccgta cacaacatca aagaactgtt cgacatggac gtggacgtac tcgactacga
     4621 aggcaaaaaa ctcatcgtcc accatatctg aacccgaaaa aaatgccgtc tgaacattca
     4681 gacggcaccc catatcctga caaaattaag acacgacacc ggcagaattg acatcagcat
     4741 aatatgcaca tattaacaga tattaatgcc gaactaccta actgcaagaa ttaaataaat
     4801 aaataaataa ataaataaat tgcgacaatg tattgtatat atgcctcctt tcatatatac
     4861 tttaatatgt aaacaaactt ggtggggata aaatacttac aaaagatttc cgccccattt
     4921 tttatccact cacaaaggta atgagcatga aacactttcc atccaaagta ctgaccacag
     4981 ccatccttgc cactttctgt agcggcgcac tggcagccac aaacgacgac gatgttaaaa
     5041 aagctgccac tgtggccatt gctgctgcct acaacaatgg ccaagaaatc aacggtttca
     5101 aagctggaga gaccatctac gacattgatg aagacggcac aattaccaaa aaagacgcaa
     5161 ctgcagccga tgttgaagcc gacgacttta aaggtctggg tctgaaaaaa gtcgtgacta
     5221 acctgaccaa aaccgtcaat gaaaacaaac aaaacgtcga tgccaaagta aaagctgcag
     5281 aatctgaaat agaaaagtta acaaccaagt tagcagacac tgatgccgct ctggatgcaa
     5341 ccaccaacgc cttgaataaa ttgggagaaa atataacgac atttgctgaa ga
//
LOCUS       DAWKST010000137         5377 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.137, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000137 DAWKST010000000
VERSION     DAWKST010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.137"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(107..1030)
                     /gene="era"
                     /locus_tag="WJL87_001653"
     CDS             complement(107..1030)
                     /gene="era"
                     /locus_tag="WJL87_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ7800064.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            complement(1077..1796)
                     /gene="rnc"
                     /locus_tag="WJL87_001654"
     CDS             complement(1077..1796)
                     /gene="rnc"
                     /locus_tag="WJL87_001654"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ7800065.1"
                     /translation="MKDDVLKQQAHAAIQKRLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDTFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRCADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVVSCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            1963..2286
                     /locus_tag="WJL87_001655"
     CDS             1963..2286
                     /locus_tag="WJL87_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ7800066.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADIAKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            2334..2810
                     /locus_tag="WJL87_001656"
     CDS             2334..2810
                     /locus_tag="WJL87_001656"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ7800067.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            2888..3313
                     /gene="nusB"
                     /locus_tag="WJL87_001657"
     CDS             2888..3313
                     /gene="nusB"
                     /locus_tag="WJL87_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ7800068.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMSDFAKADE
                     ELFNKLFFGTQTNAAEYIRQIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(3681..4715)
                     /gene="pyrC"
                     /locus_tag="WJL87_001658"
     CDS             complement(3681..4715)
                     /gene="pyrC"
                     /locus_tag="WJL87_001658"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ7800069.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAVLPEGSAFEPLMTLYLTDQATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQDILFLVHGEVTDPEIDIFDREAAFIERVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAASVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENTDTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(4775..5368)
                     /locus_tag="WJL87_001659"
     CDS             complement(4775..5368)
                     /locus_tag="WJL87_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800070.1"
                     /translation="MRMSRLMLLLSAFVLAACNAQNRQSGVRHQDFARVAVDLFRSAC
                     VLTQGSFESVSKFAAVGHFVPVGKEDLSHLPPTVAEPDVQALWTLERDGGIYYLSLTR
                     DSCSVKAERADSAALLEHFDGLVRQPPKNANIELRAEQSVQASFEIRQVSYAWREAGS
                     SEETVLTAQTADSPDLPVQAVLNLTHRSHNGKPLILP"
BASE COUNT         1252 a         1608 c         1323 g         1194 t
ORIGIN      
        1 tgcacagata atatcaaccc gtttttaaca aagatattcc cgattgaaac aaaatgccgt
       61 ctgaaacctg ttcggacggc atttgcgtaa agttcagcaa gaaaaactac aaacccagct
      121 cgcgcaggaa gcggatgtcg tccgcccaac cggatttgac tttgacccag accttcaaaa
      181 atactttggt atcaaacagt ttttccatat ccaaccgcgc ttcggtggaa attttcttca
      241 aacgctcccc gcctttgccg attaaaatcg ccttttggct ttccttatcg accaaaacgg
      301 cgatatagat gcggttcaaa ccgtcttcct cttcaaactg ctccacttcg acgttcatcg
      361 cataaggcaa ttcctcgccc aaatagcgga acaatttttc acgcacgatt tccatcgcta
      421 aaaaacgcgc cgatttgtcc gtaaccatat cttcgggata catcggcacg ctttcgggca
      481 gatacggctt gagcagctcc aacaggttgg caatccgcaa tccgtgtttc gcactgaccg
      541 cctccgccgc cgcaaattca aattcggcgc gcacttgggc aacaaacgcc tccagcgcgt
      601 aacggtcttt cgccttgtcc ttgtcgattt tgttgaccac taaaatgacc ggcgtgtgct
      661 tgggcagttg tttcaacacg acacggtcgg catcggtaaa gcgcatcgct tccacgacga
      721 aaaccaccac atccacgccg ccaagcgcct cggtaacgtt ttgattcagc ctgtcgttga
      781 gcgcgttgcg gtggtcggtt tgaaagccgg gcgtatcgac aaatacgaac tgcgcggtat
      841 cgtcggtata aatccccgtt acgcggttgc gcgtcgtctg cgcctttttg ctggtgatgc
      901 tgattttctg accgatgaga tggttcatca gcgttgattt gcccacgttc ggacgaccga
      961 caatcgccac gaagccgcaa cggtatccgc cggcggcgcg ttcccctgca aggaaggttt
     1021 caatatccat attcgctttc atatccacat ttcagacggc atattcacgc gccgcctcat
     1081 ttctttttct tcttcagcgg cagcttctct tccagccatt tcaaagcctc tttcgccgcc
     1141 tcctgttccg ccgccttgcg gctcgtccct ttggcacggc acacgaaacc cagttcgccc
     1201 aaatcgcagg aaaccacaaa catactgtcg ttggcatggc cgatttgctc ttcgatacgg
     1261 tatttcggca aagcaaagcg gcgtgcctgc aacgcctcct gcaaagcagt tttgccgtct
     1321 tttgcctgat tttggaaatc ggcacaccgg acgcggtcgg caaacaaatg gcgcaccacc
     1381 ttttccgccg tgttgaaatc ggcatcgaag ctgaccgccg caaacatcgc ctccatcgcg
     1441 tccgccagta tcgaaggccg tctgaagccg ccgctcttca actcgcccgc ccccaagtac
     1501 agaccgtcgc cgacattcat ttccgccgcc atttccgcca gcacgccctc attgaccaga
     1561 cttgcccgca accgcgacaa ctcgccctcg gtcaacttcg gaaacgtgtc aaacagcatc
     1621 cgcgccaccg tataattcaa aatcgaatca ccgacaaact cgaaccgctc gttgtgcttc
     1681 gcatgatggc tcctgtgcgt caaagcctgc cgcaaaagcg aaatatcgcg gaacgcgtag
     1741 cccagtctct tctgtatcgc cgcgtgtgcc tgctgtttca aaacatcgtc tttcattacc
     1801 gtcttccgca aaaaacagcc cgaatgcgtt tccatactcc aaaaatacag aaacacctta
     1861 aggctgcaaa cggcgcacat tgtaccacca aaccgccgcc gtctgccgac aacgcacgaa
     1921 tcgtggtaaa atccgtaagt tttcccccaa cgtattcccg taatgaacgc atttgcacaa
     1981 gcactttcct cggcactcga ccgctgtatc gccaccaaca ccgtcgccaa acaaaaccgg
     2041 cccgtcggct tcctctaccg cgaagccccc gtttttgaaa acgacagcgg ctggcgtttc
     2101 ttcagcggcg acgagaccga cgaatacacc gacgatccgg acaacttcag catcgtcagc
     2161 ctcgccgaca tcgccaaaac caaccctgaa accgccgcac tgctctccca acccgaaggc
     2221 agcgcatggg agcttgccga agacggcaca ttccaaaccg tcgccgactg gcagccgcaa
     2281 gactgaaacc ttcagacggc attccgaaac ctccgaatac caaaggacac ccaatgaaca
     2341 ccatcgcccc caacctcgac ggcaaacacc tccgcatcgg catcgtacag gcacgcttca
     2401 ccaacgaaat cggcagcgaa atgctcaaag tctgctgccg caccctccaa gaattgggcg
     2461 tggcagacga aaacattacc gtcgccaccg tacccggcgc gcttgaaatc cccatcgcgc
     2521 tgatgaactt tgcctcttcc gaaaagtttg acgcactgat tgccatcggc gtcgtcatcc
     2581 gtggcgaaac ctaccatttc gagctggttt ccaacgaatc cggagcaggc gtcagccgcg
     2641 tcgcactcga ctacaatatc ccgattgcca atgccgtcct aaccaccgaa aacgacgcgc
     2701 aggcaatcga acggattgaa gaaaaagcct cggatgccgc caaagtcgcc gtcgaatgcg
     2761 ccaacctcgt caacctgctg ctcgaagaac agtttgaaga cgaagaataa caaacagggc
     2821 gtacaccaat acgccctttc cgtttcagac ggcataccgt gccgcccaac cgtttcaagg
     2881 aaaaatcatg aaaacagccc gccgccgttc ccgcgagctt gccgtacaag ccgtttacca
     2941 atcccttatc aaccgcaccg ccgcgcccga aattgctaaa aacatccgcg aaatgtccga
     3001 ctttgccaag gcagacgaag aattgttcaa caaacttttc ttcggcacgc aaaccaatgc
     3061 ggcagagtat atccgacaaa tccgcccgct acttgacagg gacgaaaaag acctcaaccc
     3121 catcgaacgc gccgttttgc tgaccgcctg ccacgagctg tccgccatgc ccgaaacgcc
     3181 ctaccccgtc attatcaacg aagccatcga agttaccaaa accttcggcg gcacggacgg
     3241 gcacaaattc gtcaacggca tcctcgacaa actcgccgcc caaatccgcc ccgacgagcc
     3301 caaacgccgt tgatgcatca caaatttaaa gaaagttcta tgtccttatt tctgctttct
     3361 ttgtttcgtc attcccacgc aggtgggaat ttggtttttt gaattttggg tatttcaaat
     3421 aaattctgaa acttcaatct cgtcattccc gcgcaggcgg gaatccggtt ttttgagttg
     3481 ctgccgtttt tgataaactc tggtcgcatc gaatttctgg attcctgcct tcgtgggaat
     3541 ggcgaaattt gaaaattgtg ggaatttatc ggaaataacc gaaacgtaac aaactcaaac
     3601 gccgttgacg tatcaatcag tcagcaacac aaatgccgtc tgaaagccct cccttcagac
     3661 ggcattttgt ttccagccca tcaatactgc accgtccagc cgatttcgcc gcccgcgcgc
     3721 atcgggacaa gttcgccgtc gccgtaggga acacttgcgg gaacggtttg gctttgtttg
     3781 acgagggtga tcgtgtcggt attttcagga atgccgtaga accttgcgcc gttttttgag
     3841 gcgaaggctt cgagtttgtc caacgcgcct gctttttcaa atacttcggc gtaaagctcg
     3901 atagcggtca tcgcactgaa cataccggcg cagccgcagg cgttttcttt ggcggatttg
     3961 gcgtgcggcg cggagtcggt gccgaggaag aatttatgcg ccttctcgcc ggtaacggcg
     4021 gcgaccaatg cctgacggtg ggtttcgcgc ttgaggacgg gcaggcagaa atgatggggg
     4081 cgcacgccgc cgaccaagag gtcgttgcgg ttgagcagga ggtgttgcgg ggtcacagag
     4141 gcggcaacgt tgtcgcctgc ttccaaaacc aggcgggcgg cttcggcggt ggtaatgtgt
     4201 tcaaacacga ctttaagatt cggcacttgc gccaaaacgg gtttcatcac gcgctcgata
     4261 aaggcggctt cgcggtcgaa aatgtcgatt tcggggtcgg ttacttcgcc gtgaacgagg
     4321 aacaggatgt cctgttttgc catttcttcc aacacgggga tgagcttgaa taggtcggtt
     4381 acgccggaat cggaattggt cgtcgcgccg gcggggtaga gtttgaaggc gacgatgccg
     4441 gcggctttgg cttcgcgcac cagctcgggc gtggcttggt cggtcaggta aagcgtcatc
     4501 agcggctcaa acgcgctgcc ttcgggcaac accgccataa tgcgcgcttt gtaggcaagc
     4561 gcgtcggcta cactgacgac gggcggtttg aggttgggca taatgacggc gcgccccatt
     4621 tggcgggcgg tataaggggc aacggctttg agcgcgtcgc cgtcgcgcag gtgcaggtgc
     4681 atatcgtcgg ggcggataat ggtcagggtt tgcatggttt tcctttgttt tatttgaatg
     4741 ttcagacggc atgccgactt tatgccgtct gaagttaggg gagaattagg ggtttcccgt
     4801 tgtgggaacg gtgcgtcagg ttcaaaactg cctgtacggg taaatccggg gaatcggcgg
     4861 tttgtgcggt caatacggtt tcttcggagc tgcctgcttc ccgccaggca tagctgactt
     4921 ggcggatttc aaatgatgcc tgcaccgact gttccgccct caattctata ttggcatttt
     4981 tcggcggctg tcggacaagt ccgtcaaaat gttccaacag cgcggcactg tctgcccgct
     5041 cggctttcac actgcaactg tcgcgcgtca ggctcaaata ataaatgccg ccatcgcgtt
     5101 caagcgtcca caacgcctgt acatcgggtt ctgcaacagt gggcggcaga tgggacaggt
     5161 cttcctttcc gacagggaca aaatgtccga cagcggcaaa cttggaaacc gattcaaaac
     5221 tgccttgagt caggacgcag gcactccgaa acaaatcgac ggctacacgc gcgaaatcct
     5281 gatgccgtac accggattgc cggttttggg cattacacgc agcaagcaca aatgccgaca
     5341 gcaataacat caatcgggac atccgcattt cagacgg
//
LOCUS       DAWKST010000138         5341 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.138, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000138 DAWKST010000000
VERSION     DAWKST010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.138"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(103..2937)
                     /gene="lbpA"
                     /locus_tag="WJL87_001660"
                     /gene_synonym="tbpA"
     CDS             complement(103..2937)
                     /gene="lbpA"
                     /locus_tag="WJL87_001660"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HEZ7800071.1"
                     /translation="MNRRHTFPLTLTALAIATAFPAYAAQAGVAAPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTHDIQSKDKTGQFFLVEGENTL
                     KPVAKLAGYGIYLNRQLNRWVKERIEQNKPLSAEEEAMVREAQARHENLSAQSYTGGG
                     RILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAEKF
                     RDKSGVYDGDDFRDGLYFVPNIEEWKGDTNLVKGIGLKYSRTKFIDEHHRRRRMGLLY
                     RYENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSS
                     DRFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASNAISRPEQLSHNAARISEST
                     GFDDNNQDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYF
                     DFSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSLSYRASSGFRTPSFQ
                     ELFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVAD
                     HKTKLPNQAGQLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNR
                     IKPKSVSNRPDLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELA
                     YLAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAE
                     STANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(2934..5075)
                     /locus_tag="WJL87_001661"
     CDS             complement(2934..5075)
                     /locus_tag="WJL87_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ7800072.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTSPPAKPSIETTPVNQPAVGAAMRLPRRNIATFDKNGNEIPDSKQAEEYLPLKEE
                     DILFLDGTPQEQADKLKKKINERYPDVKVITSTNEDAKYNYRFVRAGYVFTRGGGYDN
                     EKEKTPDGKEFVNRFSYDGFVYYSGEHPSQSLPRTETVQYSGNWQFVTDAKRWRTQND
                     GGGINLGFSHFYGNDIGATSYAARDVDDREKHPAKYTVDFGNKTLTGELIKNQYVKPN
                     EQQKPLIIYNITAKLDGNRFTGSASVNPKLKTNYSDKEYLFFHADADQRLEGGFFGDN
                     GEELAGRFISNDNSVFGVFAGKQKTDASNASDTKPALSSGKHTKILDSLKISVDEASD
                     NNARPFAISPMPDFGHPDKLLVEGREIPLVNKEQTIELADGRKMIVRACCDFLTYVKL
                     GRIKTERPASKPKAEDKGEDEEGAGVDNGEEGEDEAVEDEGSEEDEVSEGDNGENEEE
                     IAEEETEEEVEEPEEEVEEPEEELPAEEGNGGSGSILPTPEASKGRDIDLFLKGIRTA
                     EADIPKNGTAHYTGTWEARIGVSDSGTSIQKDSYANQGAKAEFTVDFEAKTVSGMLTE
                     KNDTTPAFYIEKGVIDGNGFHALAHTRENGIDLSGQGSTNPKNFKADNLLVTGGFYGP
                     QAAELGGNIIDSDRKFGAVFGAKKDDKEATR"
BASE COUNT         1139 a         1435 c         1254 g         1513 t
ORIGIN      
        1 ggaaatcttg tttatatcga atcaaaaaaa cctgcacctt aatcagttgg cggtttagtc
       61 cgacttttgg ggtgcagatc aagctttcag acggtatttc ctttaaaact tcatttcgag
      121 cgcgaggctg aagttcctgc ccggtgcggc ataccttcca tagttgctgt cgccgccgtg
      181 ccggtttgcc gtgctttccg cagtctggcg caaggattcc caagtaacgt agcggtagtt
      241 gccgatattg tagatagccg ccctcaaggt cagccgtttt ttcagattca gataggcgga
      301 aacgtctgcc gtcgaccaag aagacgacgc tctttttgtc gaatatcgtt tttgatcgcc
      361 tgccagataa gcaagctcgt cagggttttt ccctttggaa taggtcagca taatgtttgc
      421 gccccatttc ccctctggct ggtcgtatcc gaaccccaaa acataacgcg acggctgtac
      481 cgcatccaaa gcatagctgc ggagggacaa gtccggccgg ttggataccg atttcggttt
      541 gatgcggttg tatgccaatg tggtgtacaa accttcgggc agtttgccgt acacaccgtt
      601 ccagtcgatt tttcccaata tattaacgcc ttggagcgac atattttggg cattgtaata
      661 atcgcgtata tcaatctctg tcaattgtcc tgcctgattc ggcaatttgg ttttgtgatc
      721 ggcaacggca atcatatcgg tataacggtt gcggaagctg ctgatttcca aaaagccgaa
      781 atcgcccttc cactgcaaac cgatttcgcg gttggctgcc ttttccgatt tcagggcggg
      841 acgctgccag cctttcggat aatcgtgata aatgtctatc ccgaaaagtt cttggaatga
      901 gggcgttctg aagccgctgg aggcgcggta agacaaagaa aaatgccggt tcggtttgaa
      961 cacgatgccg ctgttccacg aacggtcaac ataccgtccg ctgcggacga gttcttccga
     1021 cgtggtgaag tttttccggt cgtacctgcc acccaagctg aaatcaaaat atttgccgat
     1081 tgaaaaacgg tcgttcaaag aaatgtggat attgctgccg ttgatttttc ttggcacgca
     1141 tttgcgggaa cgcagggttt cgatgtagcc gcagaccgac ccttcgacga cttcgggctt
     1201 acccaaacgg tacttatctt gattgttatc atcgaatccc gtggattccg aaatccttgc
     1261 cgcattgtgg gaaagctgtt cagggcggga aatcgcgttg gaagcatcgt aaccgaagcc
     1321 caaagtcaga tggtgtttcg tccatttgtt tttcagcgat ttctcaaacg aggcattcaa
     1381 aacattgtgc tgttcgcggt agtggaaacg gtcgctgctg tcgtaggaat acggtttgtc
     1441 cgccgacgcg cggcaggatt tgtccacagc aggatacacg gcgcaattca gcttcagcgt
     1501 gttgttgtcg gttgccacgc cctgtttgtc aaacgacaac accgccttat ccgcccagtt
     1561 gtcagaatac gcttcgtttt cataacggta cagcaaaccc atacggcggc ggcggtgatg
     1621 ttcgtcaata aatttggtgc gggaatattt caaacctatg cccttgacca aatttgtatc
     1681 gcccttccac tcttctatat tcggcacaaa atacaagccg tcgcggaaat cgtcgccgtc
     1741 gtacaccccg ctcttgtctc taaacttttc cgcctcgtcc gtaccgtaat actgtttttc
     1801 cgtcatatcg cggatgtcgt aacgctgttt ggtatcctca aacacgccgc cgacataatg
     1861 cctgccgccg aagcggtagc ccagcttggc aagccaagag ccgctgcggt aatccatcgg
     1921 atcgggcaat atcctgccgc cgcccgtgta cgattgggcg gacaggtttt cgtggcgcgc
     1981 ctgcgcctcc cgcaccatcg cttcttcttc agcacttaaa ggcttatttt gttcaatacg
     2041 ttcttttacc cagcggttaa gctggcggtt caaatatatc ccgtagcccg ccaattttgc
     2101 cacgggcttg agtgtattct cgccctctac taagaaaaat tgccctgttt tatctttgct
     2161 ttgaatatcg tgtgtctggc ggaacgcatc caaacggttt atgccgtatg ccaccccgtc
     2221 cgcaatatcg ccgtgcgggc gcgtttcccg cccttggcgt tcggtacgga ttagcagccc
     2281 ttcccaaccg tctttgctga accccgcgcc gagcgacttc ataaattggc ggtttttact
     2341 gccgtaggcg gtttttgcct gtatccccca gcttttgccg tctgaaatca ggtctgccgc
     2401 ctctttggtg cggaaagcga ccgcgccgcc gagtgcgccg ctgccgtgat cggatgaacc
     2461 ggcacctttg tcgatttcca ccgtgctgat gttttcatat tcgatttcgt tgattgcgcc
     2521 gctgccgccg cgtccgccgt atccgctcaa cgatccctgc acggtaaacg cctgtatttg
     2581 ggcaacgccg tcaaccgaaa ccgccacacg gtttttatcc acgccgcgta tcgaataacc
     2641 gccgctcgcg ccgttgccct gttcgacaac cgccacgccc ggatcgtagc gcgtcaggtc
     2701 gcggataccg agtacctgtt ctttgttcaa cgtttccgac gttttgacga ttttgcccaa
     2761 accggtcgcc tctttcgatc gccgtcccac tttggcggca cggacggtaa cctctttcag
     2821 ggattgggtc tgcgcggcat caggtgccgc cacccccgct tgggcggcat aagccggaaa
     2881 agcggttgca atggccaagg cggtcagggt aagtgggaat gtatgccttc tgttcatcgt
     2941 gttgcctcct tgtcatcttt tttcgcccca aataccgcac cgaatttccg gtcgctgtcg
     3001 ataatattac cgcccaattc tgccgcctgc gggccataaa agccgcctgt tacaagaaga
     3061 ttgtcggctt tgaagttctt cgggttagtc gaaccctgcc cagaaaggtc aataccgttc
     3121 tcccgagtat gcgccaaagc gtggaaaccg ttaccgtcaa tcacaccttt ttcaatataa
     3181 aaagcggggg ttgtatcatt tttttctgtc agcattccgg acaccgtctt cgcttcgaaa
     3241 tcaacggtaa attctgcttt tgccccttga ttcgcatagc tatccttttg aatggacgta
     3301 ccactatccg atacgccgat acgcgcttcc caagtgccgg tataatgcgc cgttccgttt
     3361 tttggaatgt cggcttccgc cgtgcggata cctttcagga aaaggtcgat gtccctgcct
     3421 ttagaggctt ccggagtggg caggatgctg cctgaaccgc cgttgccttc ttctgccggc
     3481 aattcttctt cgggttcttc aacttcttct tcgggttctt caacttcttc ttcggtttct
     3541 tcttcggcga tttcttcttc gttttcaccg ttatcacctt cggaaacttc gtcttcttcg
     3601 ctgccttcgt cttctacggc ttcgtcttcg ccttcttcgc cgttatcaac gcctgcaccc
     3661 tcttcatcct cccctttatc ttccgccttt ggtttacttg ccgggcgttc ggtttttatc
     3721 cgtccgagtt tcacataggt cagaaaatcg caacaagcac ggacgatcat tttcctgccg
     3781 tcggcaagct cgatggtttg ttctttgttt accaaaggaa tttcacgccc ttcgacaaga
     3841 agtttgtcag gatgaccaaa atcgggcata ggggaaatgg caaacgggcg ggcattgtta
     3901 tcacttgcct cgtcaacgga aatttttaga gaatccaaga ttttggtgtg ttttccagac
     3961 gacagggcag gttttgtatc tgatgcgttt gatgcgtctg ttttttgttt gcctgcgaat
     4021 acgccgaata cgctgttgtc gttgctgata aaccgtccgg caagctcttc tccgttatcg
     4081 ccgaaaaaac cgccctcaag ccgctgatcg gcatcggcat ggaaaaacaa atattccttg
     4141 tccgaatagt ttgtcttcaa tttaggattg acactggcac tgccggtaaa gcggttgccg
     4201 tccaattttg cagtaatgtt gtaaatgatc agcggttttt gctgctcatt gggtttgaca
     4261 tattggtttt taatcagctc gcccgtcagg gttttgttac cgaaatctac cgtatatttg
     4321 gcaggatgtt tttccctgtc gtcgacatcc ctagcggcat aagaggttgc accgatatcg
     4381 ttgccgtaaa agtgactaaa ccccaaattt ataccgccac catcattttg tgtccgccaa
     4441 cgcttggcat cggtaacaaa ctgccaatta ccggaatatt gcacggtttc agtacgcggc
     4501 agggattgag acggatgttc tcctgaataa tatacaaaac cgtcataact aaatcggtta
     4561 acaaactcct taccatcagg agtcttttct ttttcattat cgtatcctcc ccccctggta
     4621 aacacatagc ccgcacggac aaaccgataa ttatattttg catcttcatt tgtcgatgtg
     4681 ataaccttca catcaggata ccgttcgttg attttctttt taagtttgtc ggcctgttct
     4741 tgcggcgtac cgtctaaaaa caggatatcc tcctctttga gcggcagata ctcctctgcc
     4801 tgcttactat cgggaatttc attaccattt ttatcaaaag ttgcgatatt ccgccttggc
     4861 agccgcattg ccgcaccgac ggcaggttga ttgaccggcg tggtttctat agaaggtttg
     4921 gcagggggcg aagtgggaac gtccttagac ttgaaagtga cggggtacgc ggtcggcgtt
     4981 gattcgacaa caggctgcac gccgaaattg ccgccaatac aagatgccaa aagtaagggc
     5041 aacaagacaa tgccgccata attcggttta cacatcccta cttttcctcc tatttgatta
     5101 ataataatta tcattatatt aatatgtaca gataatatca agccgttttt atagtgaatt
     5161 aacaaaaatc aggacaaggc gacgagccgc agacagtaca gatacatccc gtcattccca
     5221 cgaacctaca tcccgtcatt cccacgaacc tgcaccacgt cattcccacg aaagtgggaa
     5281 tccagttcgt tcggtttcgc ttgttttaag tttcgggtaa cttctacttc gtcattccca
     5341 c
//
LOCUS       DAWKST010000139         5231 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.139, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000139 DAWKST010000000
VERSION     DAWKST010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.139"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            96..179
                     /locus_tag="WJL87_001662"
                     /pseudo
     CDS             96..179
                     /locus_tag="WJL87_001662"
                     /inference="COORDINATES: protein motif:HMM:NF026862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            176..418
                     /locus_tag="WJL87_001663"
     CDS             176..418
                     /locus_tag="WJL87_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HEZ7800073.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            496..741
                     /locus_tag="WJL87_001664"
     CDS             496..741
                     /locus_tag="WJL87_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ7800074.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
     gene            744..1133
                     /locus_tag="WJL87_001665"
     CDS             744..1133
                     /locus_tag="WJL87_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800075.1"
                     /translation="MWKIIKEDSDDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDGGIADIDNIVGFVSSYSLSKSKKNALTGIAFLRGIDVYDPPISKEK
                     ALKALKKHPEIYQKFQHFFPFVELPPL"
     gene            1351..1623
                     /locus_tag="WJL87_001666"
     CDS             1351..1623
                     /locus_tag="WJL87_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800076.1"
                     /translation="MPIDNIPFYIFDLIDLKEGVGDIYNILDFVPNSDLSKDQDDALT
                     GIAFLRGIDVYDPPVSKEKALKALKKHPEIYQRFQHFFPFVELPPL"
     gene            1745..2686
                     /gene="mafA"
                     /locus_tag="WJL87_001667"
     CDS             1745..2686
                     /gene="mafA"
                     /locus_tag="WJL87_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ7800077.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYQNNPESATQYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALTRNSGRKGERSAGLSVNGTGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWMGPYSVGKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            2690..4216
                     /gene="mafB"
                     /locus_tag="WJL87_001668"
     CDS             2690..4216
                     /gene="mafB"
                     /locus_tag="WJL87_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ7800078.1"
                     /translation="MKPLRRLTNLLAACAVAAAAFGQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGQINVIQDYTHQMGNLLIQQAAIQGNLGYTVRFSGHG
                     HEEHAPFDNHAADSASKEQGNVDEGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTRSIKLNPTDTRSIRQRISDNYSNLGSNFSDRADEAN
                     RKMFEHNAKLDRWGNSMEFVNGIAAGALNPFISAGEALGIGDILYGTRYAIDKATMRN
                     IAPLPAEGKFAAIGGLGSVAGFEKNTREAVDRWIQENPNAAETVEALVNVLPFAKVKN
                     LTKAAKPGKAAVSGDFSAAYNTRTTRKVTTETEGLNRIRQNQKNSNIHEKNYGRDNPN
                     HINVLSGNSIQHILYGDEAGGGHLFPGKPGKTTFPQHWSASKITHEISDIVTSPKTQW
                     YAQTGTGGKYIAKGRPARWVSYETRDGIRIRTVYEPATGKVVTAFPDRTSNPKYNPVK
                     "
     gene            4231..4461
                     /locus_tag="WJL87_001669"
     CDS             4231..4461
                     /locus_tag="WJL87_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ7800079.1"
                     /translation="MKTLDERIKNLGKSLEDRIDANLIDAALEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
     gene            4610..>5231
                     /locus_tag="WJL87_001670"
     CDS             4610..>5231
                     /locus_tag="WJL87_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hint domain-/BECR-fold containing toxin"
                     /protein_id="HEZ7800080.1"
                     /translation="MVKTADGYKAIAHIQAGDRVLSKDEASGETGYKPVTARYGNPYQ
                     ETVYIEISDGIGNSQTLISNRIHPFYSDGKWIKAEDLKAGSRLYSESGKTQTVRNTVV
                     KPKPLKAYNLTVADWHTYFVKGNRAETEGVWVHNECPPRKTPSTPIYGNDSEAYAAAK
                     ELGYRKIKERTRNDAAIFKKGKSYISRDVDSHNGGAWKEASSPEKLN"
BASE COUNT         1669 a         1284 c         1104 g         1174 t
ORIGIN      
        1 actgtgattg aggaataagc ataattagct taatgaatag aatcagcgat atagattgga
       61 ctgcaaatcc acgcttatac gctgtgccat gattaacagg aagagagcaa cttggatggg
      121 ctagtaggga tggtaagcat ttaaatatat caattgatgg aaagattaca cacaaatgac
      181 taatttaaaa ttagatttct actctgaagt tattataaaa gattcttgcc ctaatgattt
      241 gttagaaaat ggtgagacta ttaaaggaaa aaaaggagtg gtattaggta taagcgaaga
      301 agatggtata atctatggct atactatttt actttttgat ataaaatatt gtatatatat
      361 agataagaaa tatattatac cgactggaaa aaaattctct cgcgatgatt tttattagct
      421 aatttaagta atttaagcaa gttttttaga tactgaaact ggtaattatg tttatgtaag
      481 cataaggagt tcagattgaa ggattctaca gggcaaatga ggctggcaac caaggatttg
      541 gcggaagcca ttaaacgagg agaagtacgt agttctgctt ttacaacaaa gcaactaaag
      601 gcaatcgaaa aaggagaatc taaaataccg gattacactt ggcatcatca tcaagacact
      661 ggaaggatgc aattgattaa tgaagacttg catcatgata ccggccatat cggttggaga
      721 gcgatgagta aaggaaagta actatgtgga aaatcataaa agaggatagt gatgatttag
      781 gatttgcaat taaatgctta ttctctcagt ctattgattt aaatgaattc aagttatgga
      841 ttgaacaagt aatacgcgat atgcccatcg aggacatccc tttttatatt tttgacttgg
      901 cggattttga tgggggaatt gccgatattg acaatattgt aggttttgtt tcgagttaca
      961 gcctatcaaa atcgaaaaaa aatgccttga caggcattgc cttcttaagg gggatagatg
     1021 tctatgatcc gcctatttca aaagaaaaag cattaaaagc cttaaagaaa catcctgaaa
     1081 tttatcagaa gtttcagcat ttctttccgt ttgtagaact gcccccgctt taaacagtca
     1141 aaatgccgtc tgaaatgata ttcggctttc agacggcatt tttgacacaa agcaggtaac
     1201 caaaagagcg tttgacggca aaggaagata attatgtgaa aaattacgaa agagcaaggt
     1261 caagatttaa gttttgcaac atattgctta ttatcggcag caattaattt gcaagaattt
     1321 aaattatggt tggagaaggt tgtcttagat atgcctattg ataatatacc tttttatatt
     1381 tttgatttaa ttgatcttaa agaaggagta ggagatattt ataatatttt ggattttgtt
     1441 ccgaattcag acttatctaa agaccaagat gacgcattaa caggcattgc cttcttaagg
     1501 gggatagatg tctatgaccc tcccgtttca aaagaaaaag cattaaaagc cttaaagaaa
     1561 catcctgaaa tttatcagag gtttcagcat ttctttccgt ttgtagaact gcccccgctt
     1621 taaacagtca aaataccgtc tgaaagccat ttccgccgct cagacggcat tttcgcccct
     1681 tttgtttaca aacccttaaa atccatttac actcaaactc gttcaaaaca aaacaaaccc
     1741 tgccatgaaa accctgctcc tcctcatccc cctcgtccta accgcctgcg gcacactgac
     1801 cggcataccc gcccacggcg gcggcaaacg ctttgccgtc gaacaagaac tcgtcgccgc
     1861 atcgtcccgc gccgccgtca aagaaatgga tttgtccgcc ctgaaaggac gcaaagccgc
     1921 cctttacgtc tccgttatgg gcgaccaagg ttcgggcaac ataagcggcg gacgctactc
     1981 tatcgacgca ctgatacgcg gcggctacca aaacaatccc gaaagtgcca cccaatacag
     2041 ctaccccgcc tacgacacta ccgccaccac caaatccgac gcgctctcca gcgtaaccac
     2101 ttccacatcg ctcttaaacg cccccgccgc cgccctgact agaaacagcg gacgcaaagg
     2161 cgaacgctcc gccggactgt ccgtcaacgg cacgggcgac taccgcaacg aaaccctgct
     2221 cgccaacccc cgcgacgttt ctttcctgac caacctcatc caaaccgtct tctacctgcg
     2281 cggcatcgaa gtcgtaccgc ccgaatacgc cgacaccgac gtattcgtaa ccgtcgacgt
     2341 attcggcacc gtccgcagcc gcaccgaact gcacctctac aacgccgaaa cccttaaagc
     2401 ccaaaccaag ctcgaatatt tcgccgttga ccgcgacagc cggaaactgc tgattgcccc
     2461 taaaaccgcc gcctacgaat cccaatacca agaacaatac gccctctgga tgggacctta
     2521 cagcgtcggc aaaaccgtca aagcctcaga ccgcctgatg gtcgatttct ccgacattac
     2581 cccctacggc gacacaaccg cccaaaaccg tcccgacttc aaacaaaaca acggtaaaaa
     2641 ccccgatgtc ggcaacgaag tcatccgccg ccgcaaagga ggataaaccg tgaaaccgct
     2701 gcgcagactg acaaacctcc ttgccgcctg cgccgtagcg gccgccgcct tcggacagcc
     2761 cgccctcgca gcggacttgg cgcaagaccc gttcattacc gataacgccc aacggcagca
     2821 ctacgaaccc ggcggcaaat accacctctt cggcgacccg cgcggcagcg tctccgaccg
     2881 caccggtcaa atcaacgtca tccaagacta tacccaccag atgggcaacc tgctcatcca
     2941 acaggcggca atccaaggca atcttggtta caccgtccgc ttttccggac acggacacga
     3001 agaacacgcc cccttcgaca accacgccgc cgacagcgca agcaaagaac aaggcaacgt
     3061 tgacgaaggc tttaccgtgt accggctcaa ctgggaagga cacgaacatc atcccgccga
     3121 tgcctacgac ggcccgaagg gcggcaatta ccccaaacct acgggcgcac gagacgaata
     3181 cacctatcac gtcaacggca cagcccgcag tatcaaactc aatccgaccg acacccgcag
     3241 catcaaactc aatccgaccg acacccgcag catccggcaa cgcatatccg acaattacag
     3301 caacctcggc agcaatttct ccgaccgcgc cgatgaagcc aacagaaaaa tgttcgagca
     3361 caatgccaag ctcgaccgct ggggcaacag catggagttt gtcaacggta ttgccgccgg
     3421 cgcgctcaac ccctttatca gcgcgggcga agccttgggc ataggcgaca tactgtacgg
     3481 aacgcgctat gccatagaca aagccacaat gcgcaacatc gcccccttac ccgccgaggg
     3541 caaattcgcc gccatcggcg gcttgggcag cgtggcgggc tttgaaaaaa atacgcgtga
     3601 agccgttgac cggtggatac aggaaaaccc caatgccgcc gaaaccgtcg aagccctggt
     3661 caacgtcctg ccgtttgcca aagtcaaaaa cctgacaaag gcggcaaaac cggggaaggc
     3721 tgcggttagc ggggattttt ctgctgcata caatacaaga acaactagaa aagttactac
     3781 agaaacagag gggttaaata gaatcagaca gaaccagaaa aatagtaata tacatgagaa
     3841 aaattatgga agagataatc ctaatcatat taatgtttta tctggaaatt ctatacaaca
     3901 tatactgtat ggagatgaag caggaggtgg gcatcttttt cctggcaaac ctggtaagac
     3961 aacattcccc caacattggt cagccagtaa aataactcat gaaattagtg atatcgttac
     4021 atccccaaaa acgcaatggt atgcacagac tggaacaggc ggcaaatata ttgctaaagg
     4081 aagaccagcc aggtgggtat catatgaaac gagagatgga attcgtatca gaacagttta
     4141 tgaacctgca acaggaaaag tggtaactgc attccccgat agaacctcta atcccaaata
     4201 taaccctgta aaataatgag tatttgaaag atgaaaacat tagacgaacg aataaaaaat
     4261 ttaggaaaat ctcttgaaga tagaattgac gctaatctaa ttgatgccgc actagagtac
     4321 attacttttt cagaacgttt gctagctttt gaaacgctat gtgattatat agaagatttc
     4381 aatatccagc ttacggaaaa agaatcccaa gaaatctctt ttattaataa ggaatttgga
     4441 attgaaagta catcagatta ataaatatta ggtcggttta aaaacccaac aatcaaaaag
     4501 tcgtctgaaa ctgtgtttaa tttacagacg acctttcctt catttgaagc aggatattga
     4561 gaactgagtt cttcaaaaat cctacacctg ctccttccac ggcagcacct tggtcaaaac
     4621 ggcagacggc tacaaagcca ttgcccatat tcaagccgga gaccgcgtcc tttccaagga
     4681 cgaggcaagc ggagaaacgg gatacaaacc cgttaccgcc cgatacggca atccctatca
     4741 agaaaccgtt tacattgaaa tttcagacgg catcggcaac agtcaaaccc tgatttccaa
     4801 ccgcatccat ccgttttatt cggacggcaa atggattaag gcggaagatt taaaagcggg
     4861 aagcaggctg tattccgaaa gcggcaaaac ccaaacagtc cgcaacaccg ttgtcaaacc
     4921 aaaaccgctc aaagcctaca atctgaccgt tgccgattgg catacttact ttgtcaaggg
     4981 caatcgggcg gaaacggaag gggtttgggt tcataatgag tgtccgccaa gaaaaacacc
     5041 atctaccccc atatatggga atgattcaga agcatatgca gcagcaaagg aattgggata
     5101 tagaaaaata aaagaaagaa cgagaaatga tgctgctatt tttaaaaaag gaaagagtta
     5161 tatatcacgc gatgtagatt cacataatgg aggtgcttgg aaagaggcat cttcacccga
     5221 aaaactgaac a
//
LOCUS       DAWKST010000140         5188 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.140, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000140 DAWKST010000000
VERSION     DAWKST010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.140"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(959..1204)
                     /locus_tag="WJL87_001671"
     CDS             complement(959..1204)
                     /locus_tag="WJL87_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800081.1"
                     /translation="MLKSIEFNSHIRNRLAAYLKGRGLDFQTAMREEKGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            1316..1744
                     /locus_tag="WJL87_001672"
     CDS             1316..1744
                     /locus_tag="WJL87_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ7800082.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            1756..2232
                     /locus_tag="WJL87_001673"
     CDS             1756..2232
                     /locus_tag="WJL87_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ7800083.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GQRAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSKHPFSSWKNAV"
     gene            complement(2582..3070)
                     /locus_tag="WJL87_001674"
     CDS             complement(2582..3070)
                     /locus_tag="WJL87_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ7800084.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLHVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPAIDRTHWKEAERTERRVSSKGTRYAFVHYL
                     RY"
     gene            complement(3138..4193)
                     /gene="aroG"
                     /locus_tag="WJL87_001675"
     CDS             complement(3138..4193)
                     /gene="aroG"
                     /locus_tag="WJL87_001675"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ7800085.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGSNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAG"
     gene            complement(4355..4582)
                     /locus_tag="WJL87_001676"
     CDS             complement(4355..4582)
                     /locus_tag="WJL87_001676"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800086.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCSVPD
                     CRGNGKTDVPCGFVRMNGGYCFYQSVSFSLE"
     gene            4805..5113
                     /locus_tag="WJL87_001677"
     CDS             4805..5113
                     /locus_tag="WJL87_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800087.1"
                     /translation="MTPWADAYADLRGKTKVMTTQMCASRDVSKSAKGWSVGIGLNVG
                     KQLTDSVGLEFDPYYRHKTIYKPREIVLDGDKTKMGRSKSNEYGFRVAATFYSQLKSK
                     "
BASE COUNT         1304 a         1290 c         1226 g         1368 t
ORIGIN      
        1 cagtgtcatg agaaattttc ctttgtcaag tgttaaaatt ataatgatta tatactacat
       61 tatactacat cgcaatgaaa aatcaggaaa agcaaaaaac cctccgccgt cattcccgcg
      121 aaagcgggaa tctagaattt taatgccgca agaatttatc aaggatggtc ggggtttaaa
      181 ggtctggatt cccgcctgcg cgggaatgac ggtttagaaa tcacctgaaa cgagaaaaag
      241 aaccgaaacc ggacaagtcg gattcccgca cgggcgggaa ggacggaatt tcagattgtt
      301 gtttttgatt ttctgttttg tgggaataac gggattttgg atgatgtgca tttatcggga
      361 aaactaaaaa ccctccgccg tcattcccgt gaaagcggga atctagaatt tcaatgccgc
      421 aagaatttat cggggatgtc cggggtttaa aggtctggat tcccgcctgc acgggaatga
      481 cggtttagaa atcacctgaa acgagaaaaa gaaccgaaac cagataagtc ggattcccgc
      541 acgggaggga aggacgggat ttcaggttac tgtttttgat tttctgtttt gtgggaataa
      601 cgggattttg gatgatgtgc attttcggga aaactaaaaa ccctccgccg tcattcccgc
      661 gaaagcggga atctagaatt ttaatgcggc aaaaatttat cggaaaaaac cgaagtttaa
      721 agacctagat tcccgcctgc gcgggaatga cggtttagaa atcacccgaa acgagaaaaa
      781 gaaccgaaac cgaacaagcc ggattaccgc ctgcgcggga atgacggctc aaaagttacg
      841 aaacgaaaaa taaccgaaac cagacaagtc ggattcccgc gcgggcggga atgacgataa
      901 gcgtaatttc aatctgccat attgtctttt cacttgcctg aaaacagatt tcagccacct
      961 atccctgcat ccggcggctg atgtagtcgg caatcaaatc ccgcttttcc caaaaaaact
     1021 tctgcatttt ttgttcggaa tacagtttgc ggaccagagt gggcaaaccg ctgtggacga
     1081 tggcggcgat ttctttgttg cctttttcct cccgcatcgc cgtctgaaaa tccaaacccc
     1141 tgcctttcag atatgctgca aggcggttgc ggatgtggga attgaattcg atggatttga
     1201 gcattttgcc tccgttttgc ggtaaggatt tattcgattt gtttatccgc ctgattttat
     1261 cacgtttccc aagcggtttg cctgtacaat accgcttttc ggcagggtgt gggcgatgat
     1321 gttttcttgg ttcaagctgt ttcacttgtt ttttgtcatt tcgtggtttg cagggctgtt
     1381 ttacctgccg aggattttcg tcaatatggc gatgattgat gtgccgcgcg gcaatcccga
     1441 gtatgtgcgt ctgtcgggca tggcggtgcg gctgtaccgt tttatgtcgc cgttgggctt
     1501 cggcgcggtc gtgttcggcg cggcgatacc gtttgccgcc ggctggtggg gcagcggctg
     1561 ggtacacgtc aaactgtgtt tgggcttgat gctcttggct taccagttgt attgcggcgt
     1621 gctgctgcgc cgttttcagg attacagcaa tgctttttca caccgctggt accgcgtgtt
     1681 caacgaaatc cccgtgctgc tgatggttgc cgcgctgtat ctggtcgtgt tcaaaccgtt
     1741 ttgacggagc atcttatgcc gatagaaatc gaacgccgtt ttttgattgg aaacgacaac
     1801 tggcggcaat acgccgacga accgctgctg ttgaggcagg gttatctgtc tgttgaaaaa
     1861 gaacgcaccg tccgtgtccg cattgccgga caacgggcgt ggctgacgct gaaaggctat
     1921 atttcggaaa tcagccgcag tgagtttgaa tacgaaatcc cgcttgccga tgcggaaaag
     1981 atgatggaaa cgatgtgccc gtttaaaatg gaaaaatggc gttatccggt cagatggggc
     2041 ggcagcctgt ttgaaatcga tgtttttctt ggcgataatg cgcctttggt cgtcgcggaa
     2101 atcgagttgc ccgacgagaa tgccgatttt gaccgtccgg actggctggg acgggaaatc
     2161 actgccgacg gtatgtttac caatgcttat ttgagcaaac atccgttctc aagctggaaa
     2221 aatgccgtct gaagcggctt cagacggcat tctccgacaa ccatcgcaaa tccaaagaca
     2281 gcctctatat ccttatttct gctttctttg ttccattatt cccaataaat ccccataacc
     2341 taaaatctcg tcattcccgc tcaggcggga atccggttct ttaaacttcg gttttttccg
     2401 ataaattgct ttagcttttc gtttccggat tcccgctttt gcgggaatga cgatggaaga
     2461 ttgttgttat ttcggataaa ttaccctaac tttaaaatcc cggattccca tcttcggggg
     2521 aataacaata ttttctattt cagcccatca tttgcggggg attttataga gagtttatat
     2581 ttcaatatct caaataatgc acaaaagcat agcgcgtgcc tttgctgctg acacggcgtt
     2641 ccgtccgctc tgcttctttc caatgcgtcc ggtctattgc ggggaaaaat gcatctcctt
     2701 ccacagacaa atccacttcg gttatccgca aatcggtcgc caatggcatc gcttgtccgt
     2761 atatctgcgc gccgcccata atgacggctt cttccgcgcc tgcgcacaat gccaatgccg
     2821 cctccaaact tgccgccgtt tccgcgcctg ccgcgcaata atccgcctgc cggctgatga
     2881 cgatgttcct ccgtccgggc aggggtttga cgtgcaggga ttcccacgtt ttccgcccca
     2941 taatgacggg tttgcccaag gtataggctt tgaaaaatgc gaaatcttcg ggaatgtgcc
     3001 aaggcatcgc attgcccgcc ccgatgcaca ggttttccgc acacgccgca attagggtta
     3061 ttttgagcat atttcctcca caaaaatgcc gtctgaacgc ttcagacggc attttatgac
     3121 attccgtgtc aaaaatctca accggcgcgc gccatacgtt ttttgtttgc acctgccaac
     3181 aatgccaaca gttcttcagt cgcgccccaa ccgatacacg catcggtaat gcttttgccg
     3241 tacacttccg gcttatcctg tctgccttcg accaaatggc tttccaccat cacgcccata
     3301 atattgctcc cgtcctgttc caattgggca gcaatatctt gtgccacttc catctgccgg
     3361 gtgtaatcct tgcggctgtt ggcgtggctg caatctatca tcagcttatc ggttacccct
     3421 gccgcacgca gttgttccgc cgcctcgctg acgtgtcccg catcataatt cggctctttt
     3481 ccgccgcgca aaatgacatg acagtcggga ttgccgccgg tatggacaat ggcggaatgt
     3541 ccggccttgg ttacagacaa gaaatgatgc gaatggctcg ccgcaccgat tgcgtcgatg
     3601 gcaatcttca aattgccgtc cgtaccgttt ttaaagccga cggggcagga cagcccgctt
     3661 gccaattcgc ggtgaacttg gctttcggtc gtccgcgcac cgattgcccc ccaagaaatc
     3721 aagtccgcat aatattgcgg cgtaatcata tccaaaaact cggtagaggc aggcataccc
     3781 atattgttca gcgacaacaa caggctgcgc gcctgacgca aaccgaaatt gatatcgaac
     3841 gtaccgtcca aatgcgggtc gttaatcaaa cctttccaac ccaccgtcgt cctcggcttc
     3901 tcgaaataaa cgcgcatcac aatcaaaagc tcgttttcat actgcttgcg gagtttcaac
     3961 aaacgctccg catattccaa cgccgctttc ggatcgtgaa tcgaacacgg cccgataata
     4021 accaacagcc gcttgtccct gccgtgaacc aaatcggaaa tttcctgacg ggtgcggtga
     4081 accaagcccg aagcctcttt ggaaatcggc agctcgtaaa gatgggcaat cggcggcagc
     4141 agttctttaa cttctttaat cttaatatcg tcagtggggt aatggtgtgt catcttgcat
     4201 ctttcctgat ggttttttat acgcggctta ggttacatcg tttaccttgc acaaacaagt
     4261 gcaacatatt actacaaagc tattttattt caaaataact gatatatctt ttttcataga
     4321 ttttaaattt catattttta atattttagg aattttattc caaagaaaaa gaaacagatt
     4381 gataaaaaca atacccgcca ttcattcgga caaaaccgca cggaacatcc gttttcccat
     4441 ttccacgaca atccggtaca gaacaccccg ctgccgattc cgcctgcaca tccggcaaaa
     4501 tttcatacgc aaggaaaatg ttgaaaacaa aaagaactaa ttgcttgaaa aaagatttaa
     4561 tgggaatgcg ggtatttccc acactttctc catttattta tcctcattat aaaaacatga
     4621 aaaaaaatcc tgcttgcact ctcacttgca gccgtgtccg ccgttacggt agccgatatt
     4681 cctgagtcta acggcaaggg caaagccatc atcggcgata aggaaaaaca ccgttaccaa
     4741 gcatgaaaac cttgttttga aagtctatgg cgaaatccgc caagatttgg gggacggatg
     4801 atccctgacc ccttgggcgg atgcatatgc agatttgcgc ggcaaaacca aagtgatgac
     4861 gacccagatg tgtgcttccc gcgatgtcag caaaagcgcc aaaggttgga gtgtcggtat
     4921 cggtctgaat gtaggcaaac agttgaccga cagcgtcggt ctcgagtttg atccatacta
     4981 ccgtcacaaa acaatctaca aaccccgtga gattgtcttg gacggtgaca aaaccaaaat
     5041 gggccgctcc aaatccaacg agtacggctt ccgcgtagcc gcaacgttct atagtcaatt
     5101 aaaatcaaaa taggacagta gtgcatcgtc aaatcgggcg taatcagaca aaacggtttg
     5161 cagatgcaga acgggcggta acttccag
//
LOCUS       DAWKST010000141         5150 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.141, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000141 DAWKST010000000
VERSION     DAWKST010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.141"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            397..5103
                     /gene="iga"
                     /locus_tag="WJL87_001678"
     CDS             397..5103
                     /gene="iga"
                     /locus_tag="WJL87_001678"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HEZ7800088.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADKIKQRDNAGTIKGNGEHHWNITFG
                     TNSKIGSTAVRLAGNEKDANNGQNVTFEDNGTLVLDQNINQGAGGLFFKGDYTVKGIN
                     NDITWLGAGIDVTDGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVI
                     LNQQADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFE
                     HIRNVDEGARIVNHNTSHASTITLTGKSLITNPNSLSVHSIQNDYDEDDYSYYYRPRR
                     PIPQGKDLYYKNYRYYALKSGGSVNAPMPENGVTENNDWVFMGYTQEEAKKNAMNHKN
                     NQRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGKISVTQGNVLLS
                     GRPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKAAEIAVNQSASFSSGRNV
                     SNITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALNSFGATQINGN
                     VNLNQNAALVLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLSLADSHIHLNN
                     ASDAQSANQYHTLKINHLSGNGHFHYLTHLAENLGDKVLVKESASGHYQLHVQDKTGE
                     PNQEGLNLFDASSVQDRSRLSVSLANNHVDLGALRYTIKTENGITRLYNPYAENRRRV
                     KPVPSPATNTASQAQTDSAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKRQQAE
                     AERKSAELAKQKAEAEREARELATRQKAEQERSSAELARRHEKEREAAELSAKQKVEA
                     EREAQALAVRRKAEAEEAKRQAAELARQQEEARKAAELAAKQKAETERKAAEIAEQKA
                     EAEREAAELAKQKAEEEGRQAAQSQPKRRNRRAIPPELSSDATTRALPRIARNSNPDA
                     SDYEEIPLDALEDEDVSESVDTSDKQPQDNTELHEKVETVSLQPRAAQPRAQAAAQPQ
                     AQAAAQPQAQAVAQADAVSTNTNSALSDAMASTQSILLDTGASLTRHIAQKSRADAEK
                     NSVWMSNIGYGRDYASAQYRRFSSKRTQTQIGIDRSLSENMQIGGVLTYSDSQHTFDQ
                     ASGKNTFVQANLYGKYYLNDAWYVAGDIGAGSLRSRLQTQQKANFNRTSIQTGLTLGN
                     TLKINQFEIVPSAGIRYSRLSSADYKLGNDSVKVSSMSVKTLTAGLDFAYRFKVGNLT
                     VKPLLSAAYFANYGKGGVNVGGNSFAYKADNQQKYSAGAALLYRNVTLNVNGSITKGK
                     QLEKQKSGQIKIQIRF"
BASE COUNT         1738 a         1207 c         1118 g         1087 t
ORIGIN      
        1 acggaaacct gcaccgcgtc attcccacga acccacatcc cgtcattacc acgaaagcgg
       61 gaatctagga cgcagggtta agaaaaccta catcccgtca ttccctcaaa aacagaaaac
      121 caaaatcaga aacctaaaat cccgtcattc ccgcaaaagc gggaatccaa aaacaaaaag
      181 ccgcagaaat ttaacggaag aaccgaaccc cgaaaatcgg cttcccgcct tcgcttgaat
      241 aacagaacaa aggaagcgga aataaaaaca tcgggattct ttttttaaat tggcaataaa
      301 acaccaaaat taatgattca cacaacaaaa aatagtcaac accgccaaac cggcgtaaaa
      361 tacaaaacat tatcaccctt aaaacggtaa aaccttatga aaaccaaacg ttttaaaatt
      421 aacgccatat ccttatccat ctttcttgcc tatgccctta cgccatactc agaagcggca
      481 ttggtcagag acgatgtcga ttatcaaata ttccgtgact ttgcagaaaa caaaggcaaa
      541 ttttttgtcg gcgcaaccga tttatcagtg aaaaacaaac aaggtcaaaa catcggcaac
      601 gcattatcca acgtaccgat gattgatttt agcgttgcag atgtcaacag acgtacatta
      661 accgtcatcg atccccagta tgccgtcagc gtcaaacatg taaaaggaga cgaaatctct
      721 tattacgggc atcacaatgg gcacttagat gttagcaatg atgaaaacga atatcgttca
      781 gttgcacaaa atgactacga accaaataaa aattggcatc acggtaatca aggtcgtctt
      841 gaagactaca acatggcacg ccttaataaa ttcgtaacag aagtcgcacc tattgcacca
      901 accagtgcag gcggcggcgt tgaaacctat aaagataaaa accgtttttc tgagtttgtg
      961 cgagttggag caggtacgca atttgaatat aacagccgct acaatatgac agagctttca
     1021 cgagcttatc gttatgccat tgcaggtacg ccttatcaag acgtgaatgt cacatcgaat
     1081 ctaaatcaag aaggcttgat tggcttcggt gataattcaa aacatcattc cccagaaaaa
     1141 ctcaaagaag ttttatctca aaacgcattg actaactatg ctgtgttagg cgatagcggt
     1201 tcaccattat ttgcttatga taaacaagaa aaacgctggg tctttttagg tgcttacgat
     1261 tattgggcag gctatcaaaa aaactcttgg caagaatgga atatctataa aaaagaattt
     1321 gcagataaaa tcaaacaacg cgataacgcc ggcaccatca aaggtaatgg agaacatcat
     1381 tggaacatta cttttggcac aaacagcaag attggttcta cagcggtaag gcttgccggc
     1441 aatgaaaagg atgcaaacaa cggacagaat gttacctttg aagacaacgg cactttggta
     1501 ttggatcaaa acatcaacca aggcgcgggc ggtctgtttt tcaaaggcga ttacacagtc
     1561 aaaggtataa ataatgacat cacttggcta ggtgcgggga ttgatgttac cgacggcaaa
     1621 aaagtcgttt ggcaagtcaa aaatccgaat ggcgacagat tggcaaaaat cggcaaaggc
     1681 acattagaaa taaacggcac aggcgttaac caagggcaat taaaagtcgg cgacggtacg
     1741 gttattctga atcaacaagc cgatgccgac aaaaaagtcc aggctttctc ccaagtcggc
     1801 attgtcagcg gacgcggtac attggtatta aatagttcaa atcagattaa tcccgataac
     1861 ctatatttcg gtttccgtgg cggtcgtttg gatgccaatg gcaatgactt gacttttgaa
     1921 cacatccgca acgtggatga aggcgcgcgc attgtcaacc acaacacaag ccacgcctcc
     1981 acaatcacgc taacgggtaa atctttaatt accaatccga acagcctttc ggtacatagc
     2041 atacaaaatg actatgacga agatgattat agttattact accgaccaag gcgcccgatt
     2101 ccgcaaggca aagaccttta ttacaaaaac tatcgttatt acgccctaaa atccggcggc
     2161 agcgtgaacg caccgatgcc cgagaacggt gtaacagaaa ataacgactg ggtctttatg
     2221 ggatatacgc aagaagaagc caagaaaaac gcgatgaacc acaaaaacaa tcagcgtatt
     2281 agcggtttta gcggtttctt tggcgaagaa aacggaaaag gacataacgg tgcattaaac
     2341 cttaatttca acggcaaaag cgcgcaaaac cgtttcttgt taacaggcgg cactaattta
     2401 aacggaaaaa taagcgtaac tcaaggcaat gtcttgttat caggtcgtcc aacaccacac
     2461 gcaagagatt ttgtgaacaa atcttcagcc cgaaaagacg cacatttctc caaaaacaat
     2521 gaagtcgtat ttgaagacga ctggataaac cgcacattca aagccgcaga aattgcggtt
     2581 aatcaatccg catcattctc ttccggtaga aatgtatcca acattaccgc caacatcacc
     2641 gcgacagaca acgccaaagt aaatttgggt tacaaaaacg gcgatgaggt ttgcgtgcgc
     2701 tcggactata ccggttatgt tacctgcaac acaggcaact tatccgataa ggctttaaac
     2761 agctttggag cgacacagat taacggcaat gtgaatctga accagaatgc ggcattggtc
     2821 ttgggcaagg ctgcattatg ggggcaaatt caagggcaag gaaacagccg tgtcagccta
     2881 aaccaacata gcaaatggca tttgactggc gacagccaag tacacaatct gtcattggcg
     2941 gatagccata ttcatttgaa caatgcctcc gatgcgcaaa gtgcaaatca ataccacacg
     3001 ctcaaaatca atcatttatc cggtaacggg cattttcatt atctgacgca cttggcggaa
     3061 aatcttgggg ataaagtgct tgtgaaggaa tccgcatccg gtcattatca gctccatgtt
     3121 caagataaaa caggcgaacc caatcaggaa gggctgaatc tctttgatgc atcatccgta
     3181 caagaccgct cccgcctttc tgtttccttg gcgaataacc atgtagattt aggcgcattg
     3241 cgttatacaa tcaaaacaga aaacggtatt acccgattgt acaatcctta tgccgagaac
     3301 cgccgccgag tcaaaccggt cccgtctcct gccacaaaca cggcttctca agcacaaacg
     3361 gacagtgcac aaattgccaa gcctcaaaat atcgtcgtcg caccgcctag cccgcaggca
     3421 aatcaagccg aagaagcaaa acgccaacaa gcaaaagcgg agcaagtgaa gcgtcagcaa
     3481 gcagaagcag agcgtaaatc ggcagagtta gcaaaacaaa aagcagaagc cgaacgcgaa
     3541 gcaagagagc tggcgacccg ccaaaaagca gaacaagaac gcagcagcgc cgaacttgca
     3601 cgtcgacatg aaaaagaacg cgaagcggca gagttgtcgg caaaacaaaa agtcgaagcc
     3661 gaacgtgagg ctcaagcatt ggcggtacgc cgaaaagcag aagccgaaga ggcaaaacgc
     3721 caagctgcag agcttgcacg ccaacaagaa gaagcacgaa aagctgcaga gttggctgct
     3781 aagcaaaaag cggaaactga acgcaaagca gcagaaattg ccgagcaaaa agcggaagcc
     3841 gaacgtgaag ccgccgaact tgccaagcaa aaggcggaag aagaaggtcg ccaagcagca
     3901 caatctcaac ccaaacgtcg taaccgcagg gctataccac cggaactgtc atctgatgca
     3961 acaacccgag ctttgcctag gatagcgaga aattcaaatc ctgatgccag cgattatgaa
     4021 gaaatcccct tggatgcgtt ggaagatgaa gatgtatccg aatcggttga cacatcagac
     4081 aaacagcctc aagacaatac ggaacttcat gaaaaagttg agacagtgag tttgcaacca
     4141 agagccgcgc agccgcgagc tcaagccgcc gcgcagccgc aagcccaagc cgccgcgcag
     4201 ccgcaagccc aagccgtcgc gcaagccgat gcagtcagca ccaataccaa ctcggcttta
     4261 tctgacgcaa tggcaagcac gcaatctatc ttgttggata caggtgcttc attaacacgg
     4321 cacattgcac aaaaatcacg tgctgatgcc gaaaaaaaca gcgtttggat gtcgaacatc
     4381 ggttatggcc gtgattatgc ttccgcacaa tatcgccggt ttagttcgaa acgcacgcaa
     4441 acacaaatcg gcattgaccg cagcttgtcc gaaaatatgc agataggcgg cgtattgact
     4501 tactctgaca gtcagcatac ttttgatcag gcgagcggca aaaatacttt tgtgcaagcc
     4561 aacctttatg gtaagtatta tttaaatgat gcttggtatg tggccggcga tattggtgca
     4621 ggcagcttga gaagccggtt acaaacacag caaaaagcaa actttaaccg aacaagtatc
     4681 caaaccggcc ttactttggg caatacgctg aaaatcaatc aattcgagat tgtccccagt
     4741 gcgggaatcc gttacagccg cctgtcatct gcagattaca agttgggtaa cgacagtgtt
     4801 aaagtaagtt ctatgtcagt gaaaacactg acggccggac tggattttgc ttatcggttt
     4861 aaagtcggca accttaccgt aaaacccttg ttatctgcag cttactttgc caattatggc
     4921 aaaggcggcg tgaatgtggg cggtaattcc ttcgcctata aagcagataa tcaacagaaa
     4981 tattcagcag gtgccgcgtt actgtaccgt aatgttacat taaacgtaaa tggcagtatt
     5041 acaaaaggaa aacaattgga aaaacaaaaa tccggacaaa ttaaaataca gattcgtttc
     5101 taaaatacta aattcatagc aaaataaaat gccgtctgaa ctcaggcttc
//
LOCUS       DAWKST010000142         5052 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.142, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000142 DAWKST010000000
VERSION     DAWKST010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.142"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            61..1164
                     /gene="mnmA"
                     /locus_tag="WJL87_001679"
     CDS             61..1164
                     /gene="mnmA"
                     /locus_tag="WJL87_001679"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ7800089.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDEETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            1235..2905
                     /locus_tag="WJL87_001680"
     CDS             1235..2905
                     /locus_tag="WJL87_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ7800090.1"
                     /translation="MEKIWLDSYEKGVSAEIDITQYNSISDVFRQSVEKFARLPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMMPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGTTAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVVKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIILITNPRDMKGFIGELKKQRINVFIGVNTLFNAMVNRPDFAEVDFSELRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLIVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVRKDPS
                     LTKEELIAFCRTELTAYKVPKDIEFRDELPKSNVGKILRRELRQSTGK"
     gene            3017..4651
                     /locus_tag="WJL87_001681"
     CDS             3017..4651
                     /locus_tag="WJL87_001681"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEZ7800091.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSEHIRERHRHRYEVNNNYVPTLEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
     gene            4879..5052
                     /locus_tag="WJL87_001682"
                     /pseudo
     CDS             4879..5052
                     /locus_tag="WJL87_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         1331 a         1390 c         1251 g         1080 t
ORIGIN      
        1 gcctttaaaa tgtgttaaaa tctgccttta aaaaccgccg tttccaacca tattctacgc
       61 atgaatacga cagcaaaccc ttccaatatc atcgtcgggc tctccggcgg tgtcgattct
      121 tccgtaaccg ccgccctgct caagcagcag ggttatcaag tgcgcggtgt gttcatgcag
      181 aactgggaag acgacgacaa cgacgaatat tgcagcatca aacaggattc gttcgatgcc
      241 atcgccgttg ccgatattat cggcatcgat atcgacatcg ttaatttcgc cgcgcaatat
      301 aaagacaaag tctttgctta ttttcttcag gaatacagtg cggggcgcac gccgaatccg
      361 gatgtgttgt gcaacgccga aatcaaattc aaatgctttt tggactacgc cgtagggcag
      421 ggcgcggata ccatcgccac cggccactat gcgcgcaaag aagttcgcaa cggcgtgcat
      481 tacctgctca aaggtttgga tcgaaacaaa gaccaaagct atttcctcta ccgcctcaag
      541 cctttccaac tcgagcgcgc gatttttccg ttgggcggtt tggaaaaacc tgaagtccgc
      601 cgccttgccg ccgaatttaa tttgccgacc gccgccaaaa aagacagcac gggcatctgc
      661 ttcatcggcg aacgcccgtt tcgcgagttt ctgcaaaaat acctgccgac cgacaacggt
      721 aaaatggtta cgcccgaagg gaaaaccgtc ggcgaacacg tcgggctgat gttctacacg
      781 ctcggacagc gcaaaggttt gggcatcggc ggcgcgggcg aaccgtggtt tgttgcggct
      841 aaagatttga cgaaaaacga actgattgtc gtgcaaggac acgaccatcc gctgctctat
      901 acccgcagcc ttgtgatgaa cgatttgagt ttcacgctgc ccgaacgccc gaaggcagga
      961 cgctatacct gcaaaacgcg ttaccgtatg gcggacgcgc cttgtgagtt gcgctatttg
     1021 gatgaagaaa ccgccgagct ggtgtttgac gaaccgcaat gggcggttac gccgggccag
     1081 tctgccgtgc tgtacgacgg cgatatctgt ttgggcggcg gcatcatcca aacgaccgac
     1141 aaacccgtca tcatcacgcg ataaaggtaa tgccgtctga aacggttttc agacggcatt
     1201 gttccgctca attccacttt aaagaccgat acctatggaa aaaatctggt tagacagtta
     1261 cgagaagggc gtcagtgccg aaatcgatat cacgcaatac aattccatca gcgatgtatt
     1321 ccgccaaagc gtggaaaaat ttgccaggct gcccgctttt caaaatatgg gcaaaacgct
     1381 gacttatgcc gaaaccggca aactggcgac tgatttcgcc tcttatctgc aaaacgtcct
     1441 caagctgccg cgcggcgaac gtgttgccat tatgatgccg aacgtattgc aatacccgat
     1501 agcccttttc ggcattttgc aggcaggttt ggtggcggtg aacaccaatc cgctctatac
     1561 gccgcgcgag ctggagcatc agctgaaaga cagcggcacg accgccatca tcgttttgga
     1621 aaatttcgcc aacacgctgg agctggtgct gccgcgcacg cagatcaaac atgtcatcgt
     1681 cgcctccgtc ggcgaaatgt tcgggctgct taaaggttcg ctgatcaatt tcatcatccg
     1741 aaaaatcaag aaaatggttc ccgaataccg tattcgggaa accgtttcct ttcagacggc
     1801 attgaaagag ggggcgaagc acgttttcca acctgtcgca ttaaaccgcg aagataccgc
     1861 gctgttgcaa tacacgggcg gcacgacagg tgttgtcaaa ggggcggtgc tgagtcacgg
     1921 caacatctgt gccaatatgc ttcaggcaaa agaatggatt aaaaaccagt tgcgcgaggg
     1981 caaagaaacc gttatcgctg ccctgccgct gtaccatatt ttcgccttaa ccgtgaatct
     2041 gatgattttt gccaatgccg gctcaaaaat catcctgatt accaacccgc gtgatatgaa
     2101 aggctttatc ggagaactga aaaaacagcg gattaatgta tttatcggcg taaacacact
     2161 gtttaacgcg atggttaacc gtcctgattt tgccgaagtc gatttttcag aattgcggct
     2221 gactttgggc ggcggtatgg cgacccaaaa agccgttgcc gaaaaatgga aaaaaattac
     2281 cggtacgcct attgtcgaag cctacggtct gaccgaagcc agcccaggcg tgtgctgcaa
     2341 ccccttaaac atcgaatcat acagcggcag catcggtttg cccgtctcgt ccaccgaagt
     2401 cgaactgcgc gacgcaaacg gcaaagaagt ccccgtcggg cagccgggcg aattgtgggt
     2461 aaaaggccct caagtgatgc aaggctactg gaaccgtccc gaagaaaccg ctaaagccat
     2521 agacgcgcgc ggtttcttgg aaaccggcga tattgccgtc atggacgaaa aaggctggtt
     2581 gaagctggtt gaccgtaaga aagacctcat tgttgtttcc ggattcaatg tttatccgaa
     2641 cgaaatcgaa gaagtcatcg cgcatcacgg caaagtgatg gaagttgcct gtatcggcgt
     2701 tcccgacgaa aaaaccggcg aggcactcaa agtattcgtc gtcagaaaag acccgtcttt
     2761 gacgaaagaa gaacttatcg ccttttgccg caccgaatta accgcatata aagtaccgaa
     2821 agatatcgaa ttccgcgacg agttgcccaa gtccaacgtc ggcaaaatcc tgcgccgcga
     2881 gttgcggcaa agtaccggga aataaagaaa agatgccgtc tgaaaacagc cgtccaccgt
     2941 tcagacggca ttcgtccgtt tgccagaacc gcgcgcttca cgttaaaatc acgcattcca
     3001 atatgggtat tccatcatga ccaaattcat tttcgtaacc ggcggcgttg tctcctcact
     3061 gggtaaaggt atcgccgccg cttctattgc cgccatcctc gaatcgcgcg gcttgaacgt
     3121 taccatgctc aagctcgatc cttatatcaa cgtcgatccc ggcacgatga gcccgttcca
     3181 acacggcgaa gtgttcgtaa ccgacgacgg cgcggaaacc gacctcgact tgggacacta
     3241 cgaacgcttt atcgattcca cgatgacccg ccgcaacagc ttcagcacgg ggcaggtgta
     3301 cgaaaacgtc atcgccaaag aacgacgggg cgactatttg ggcggcacgg ttcaagtcat
     3361 tccgcacatt accgacgaaa tcaaacgccg catccacgaa ggcgcggcag gttacgatgt
     3421 ggcgattgtc gaaatcggcg gtacagtcgg cgatatcgaa tctttgccat tcttggaagc
     3481 catccgccag atgcgaagcc agttgggacg caacaacacc ctgttcgccc acttgagcta
     3541 cgtcccctac atcgccgctg caggcgaaat caaaaccaag ccgacccagc acaccgttaa
     3601 agaaatgttg agcatcggtt tgcaacccga catcctgatt tgccgtatgg acaggacaat
     3661 gcctgcggac gaacgccgca aaatcgcctt gttctgcaac gtggaagaac gcgcgattgt
     3721 cggcagctac gatgtggaca gcatctacga atgccccgaa atgctgcacg accaaggcat
     3781 cgacaacatc attaccgagc agttgcagct taatgtgcag caggcggatt tgaccgcgtg
     3841 gaaaaaaatc gtccacgcca tccaaaaccc gaaacacacc gtcaaaatcg ccatggtcgg
     3901 caaatacgtc gatttgaccg agtcctacaa atcgctgatc gaagccttga aacacgcagg
     3961 cattcacacc gaaaccgacg tacaaattac ctttgttgac agcgaaagca tcgagaaaaa
     4021 caacggcgac gtttccatgc ttaaagacat ggatgccatc ctcgttcccg gcggttttgg
     4081 ttcgcgcggc gtggaaggca aaatcgccgc tgtgcgctat gcccgcgaaa acaacgtgcc
     4141 atacttgggc atctgcctcg gtatgcagat tgcgctgatc gaatacgccc gcgacgtggc
     4201 aggtttgaaa ggtgcgaatt ccaccgagtt cgacctcaaa tgcgccgccc ccgtcgttgc
     4261 cctgattgac gaatggcaaa ctgccgacgg cagcgtcgaa acccgcgacg aatccgccga
     4321 tttgggcggc accatgcgtt tgggcgcgca agaagtcgaa ttgaaagcag gcagcctcgc
     4381 cgccaaaatc tacggcagcg aacacatccg cgaacgccac cgccaccgct acgaagtcaa
     4441 caacaactac gttcctacgc tggaacaggc aggtttggtc atcggcggcg tatccgccgg
     4501 acgcgaacgc ttggtcgaaa ccatcgaact gccgaaccat ccttggttct tcgcctgcca
     4561 gttccacccc gagtttacct ccaatccgcg caaagggcat cctttgttca ccgcgtttgt
     4621 caaagctgcg ttgaacaata aaaaagcctg ataaagcgtt acttgatgat caaaatgccg
     4681 tctgaaagcc tgaatgaaga ctttcagacg gcatttttgc aaatcgggga tcataaaagc
     4741 catcaattta atttttatgg ttttatctaa tattgaccaa ctgaatattg ttaagcgggg
     4801 cgtgaaactc aagttgagac ctttgcaaaa ttccccaaaa tcccctaaat tcccaccaag
     4861 acatttagga gatttctcat gagcaccttc tttcagcaaa ccgcacaagc catgattgcc
     4921 aaacacatcg accgcttccc actattgaag ttggaccagg tgattgattg gcagccgatc
     4981 gaacagtacc tgaatcgtca aagaacccat ttaccttaga gaccaccgcg gccgtcccgc
     5041 ctatccgctg ct
//
LOCUS       DAWKST010000143         5013 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.143, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000143 DAWKST010000000
VERSION     DAWKST010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.143"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(244..2520)
                     /locus_tag="WJL87_001683"
     CDS             complement(244..2520)
                     /locus_tag="WJL87_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ7800092.1"
                     /translation="MAQTTLKPIVLSILLINTPLLAQAHETEQSVDLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDSALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGAAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDDAHAHAHNGKPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYHHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLGQKSSALSAIPETVKQPMLIDNNVRHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIQYDKALIDRENYYNQPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVQGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGIHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            2942..3532
                     /locus_tag="WJL87_001684"
     CDS             2942..3532
                     /locus_tag="WJL87_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ7800093.1"
                     /translation="MLLFIDNYDSFTYNIVQYFTELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIDRNTMPECLEVTAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLVEFQNFKPQKI"
     gene            3596..4654
                     /gene="trpD"
                     /locus_tag="WJL87_001685"
     CDS             3596..4654
                     /gene="trpD"
                     /locus_tag="WJL87_001685"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ7800094.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVVEQMGANLNLTPEQVAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLEGREGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(4806..>5013)
                     /locus_tag="WJL87_001686"
     CDS             complement(4806..>5013)
                     /locus_tag="WJL87_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ7800095.1"
                     /translation="TVVVPNAQSATLLPIIRKKVKPDGIVYTDTFRSYDVLDISEFSH
                     LRKFNGIPKEHLGLHLKKCQWHFK"
BASE COUNT         1142 a         1149 c         1426 g         1296 t
ORIGIN      
        1 ctgaaaccga atgaattgga ttcccgcctg cgtgggaatg atgggatctt gggtttttgc
       61 ttttgatttt tctgcttttg cgagaatgac ggcgtgaaag caagaatgat gaaacaaaaa
      121 aaatgggaat gatggcatag tggtttgttc tttgtctttg ccatatttcc taacaaattg
      181 attaaaaaga aaaaaggttt tcagaatgcc gtctgaaaac cttttttgtt tgcctgtccg
      241 attttaaaac ttcacgttca cgccgccggt aaagctgcgg cccatttgcg gcgtatcaga
      301 gaggaagctg ctgtgggcgt aaacggattg gttgagcagg ttgtcggctt tgacgtacca
      361 attccactcg ccatagcgcg tattgcggcg gtagtttgcg ccgaggttga gcatatggtg
      421 tccgggcgtg cgcgtttcgt agcgggcgag tttgttttgg gcgaacacgc ggtagtagtc
      481 caaattggca tcgatacggt cggtcagcga ggctttcagg tggatgccga ggcgcgcagc
      541 cggaacgcga ggcgcgtttt ggtcgtcctg tgcaatgaaa ggacggttgc cgtaagcatc
      601 ttccctgccg ggtagggatg gcaggttttt cagacggcct tgtacatagt cgccggaaac
      661 gccgatgcgg tagcgcggtg tgggcttgaa gtagatttcg ccttctgcac cgtagaagtc
      721 cgcaccggat tggttgtagc gcacgagctt catttcgctg tcgtcttcga tggatttggg
      781 gccgcgtccg tcgtttaagg tttgggcgta aatgtagttg ccgaaacggt tgcggtagag
      841 tgccagattg tattgccagc ggtcgccttc gtagcccagc gcgagttcga tgttgttgga
      901 acgctcttta ttgaggtgtt tgttgccgac ttcaaaagtg ttggtggcga cgtgcttgcc
      961 gtgtgcgtac agctcttgcg ttgacggcag gcgttcctga tgggaggcgg tcagactgag
     1021 tttgtgttgt ggcgtgaaat accagttgcc cgaaagtgcg aacgagcggg cggtttggcg
     1081 gtgcgcgccg aggtcgggca ggggttggtt gtagtagttt tcccgatcaa tcaatgcttt
     1141 gtcgtactgg atggaggctt tttgtttttc cacgcgtacg ccgccttcaa gcgtgaagtt
     1201 gtcccagttt gcctgttcta caccgaaaaa gctgtaatgg cgtacattgt tgtcaatcaa
     1261 catcggttgt ttgacggttt cgggaatggc ggaaagcgcg ctggattttt gtcccaaata
     1321 ttgcacgccc cagctgcctt tcagacggcc tatgggttgg tggcgcaact cgatacgggc
     1381 gttttgcgtt tggttgttaa aaaagttttc gactgcatcg cctgcttttt cgtcgtggtg
     1441 gtagtcgttg cggttcaggt gtacgcgcag ggcttcaaaa ccggggaatg gctgcttcca
     1501 ttcggcgcgg agttcgtagc gtttgttgcg caggtctatc caaggtttgc cgttgtgggc
     1561 atgggcgtgt gcatcatcgt cgtcgtgaaa gccgcagctc aagcccggat tgtcgtaatc
     1621 gatgtcttct tcggtcaaca ggtgcggata aagctgcaaa tagcgtttgt taatcaaact
     1681 cttttgccag atgatgtcgg cgtggcaatc atcgtattcg tggctgtggg caggcagacc
     1741 atattggtcg cgacggtcgc tgtatgctgc gccgataaag cctttttcgc caacccaaga
     1801 cagcccgatg ctgcccgttt gcgaatcggc gtggctgtcg ggcaggcgtt tcagattgcg
     1861 gtaacgcggt acggcgtaat cgcccgattt gcggtacaag ccttccgtgt gcaatacaaa
     1921 gtttttgccc aaaccgatat tgatgccgcc ggacgtgagt ttttccagat tgccgctgct
     1981 caaacgcaat ccgagttcgc ccgatacgcc gttttcaggc attttttcgg ggattttgcc
     2041 atcggcaaca tcgaccagcc ccgccacatt gcccgagctg tacaagagcg taaccggacc
     2101 gcgcaggatt tcgacctgtt gcgacaaggc gctgtccacc atgattgcat ggtctggaga
     2161 gaagtccgcc atgtcgcccg tttcgccgtg atggttcaac actttaatcc gcctgcctgt
     2221 ttgaccgcga atgacgggag cagacgcgcc gccgccgtat tgcgaagcgt ggatgcccgg
     2281 tacgccgtct aaagcgtcgc ccaagttgac ggctttttgg cgcaaggtat cgccggagat
     2341 gattttgtcg gaggcggtcg aagtgtgcaa cagccccgac gtggcgcgcg gacggctttt
     2401 gccgacgacg ctgaccgttt ccaaatccac cgattgctca gtttcatgcg cttgggcgag
     2461 gaggggtgtg ttgattaaaa gaattgataa aacaatgggt ttgagtgtag tttgtgccat
     2521 tttggcttct cgtcgcattt caaaagtttg ttattatata acattgcatt ccttatatca
     2581 taagattttg agaacactca gagggcatag gcaaaagttt ttcaaatgaa acggttgcgg
     2641 catcgggcgg tgtccatttg tatccgccgt ccttcggggg cgcggttgat gttgacgcag
     2701 attccgctgt gtttgcccat tatgttcggc tgcgggataa ccaaaattta tgagtgcata
     2761 aaaacggcac gttcccgaac ggtcgaaaag gtggcaaaat ggcgtacttg tcagaacgcg
     2821 aggctctgcg ccaggttcac gagggcgcat cgggcacgct aagatataag agggtatgga
     2881 tgggggtatc ggaaatgcag ttaataacaa acaaattata aatcaatagg ttaatcacaa
     2941 aatgcttttg tttatcgaca attacgacag ttttacttac aacatcgtcc agtatttcac
     3001 tgaattgggg caggaagttg ccgtgcgccg caacgatgat attacgttgg aggaaatcga
     3061 ggcattgaat ccgcaatatc tcgttatcgg ccccggcccg tgttcgccca aagaagcggg
     3121 gatttccgtg gcggcgatgc gccatttcgc cggccggctg ccgattatgg gcgtgtgcct
     3181 cgggcatcag acgataggcg aggcgttcgg cggcaggata gtccgcgcca aaacgctgat
     3241 gcacggtaag gtgtcgcccg tgtcccattc gggcaagggt atgtttaagg gtttgcccaa
     3301 tccggttacc tgtactcgtt atcacagcct cgttatcgat cggaatacga tgcccgaatg
     3361 tttggaagta acggcttgga ctgaggacgg cgagattatg ggtgtgcgcc ataaggaata
     3421 tgccgtcgag ggcgtgcagt tccaccccga agctctgctg accgaacacg gacatgatat
     3481 gttaaacaat tttttagttg aatttcaaaa cttcaaaccg caaaaaatct gacgtgatgc
     3541 cgtctgaatc ccttcagacg gcattttcgt ccgaatattg aacggaggac aaaaaatgat
     3601 tacaccgcaa caggctatcg aacgattaat cagcaataat gagttgtttt acgatgaaat
     3661 gaccgacttg atgcgccaaa tgatgagcgg aaaagtgccg cccgaacaaa ttgcggcgat
     3721 tttgacagga ttgcgtatca aggttgaaac cgtttccgaa attaccgcag ctgcagccgt
     3781 catgcgtgag tttgcgacaa aagtgccgct ggagaatgca gaggggctgg tcgatatcgt
     3841 cggtacgggc ggggatggcg cgaaaacctt caatatttcg acgacttcga tgtttgttgc
     3901 cgcagcggca ggcgcgaagg ttgccaaaca cggaggccgg tcggtctctt cctccagcgg
     3961 tgcggctgac gtggtggagc agatgggcgc aaacctcaac ctgactcccg aacaggttgc
     4021 ccaaagtatc aggcagaccg gcatcgggtt tatgttcgcg cccaatcacc acagtgccat
     4081 gcgccatgtc gcccctgtac gccgttcgct cggtttccga agtattttca acatattggg
     4141 tccgttaacg aatcctgcgg gcgcgccgaa ccagcttttg ggcgtgttcc acaccgattt
     4201 gtgtggcatt ttgtcgcggg tcttgcaaca acttggttca aaacacgttt tggttgtttg
     4261 cggggagggc ggtttggatg aaattacact gacgggcaaa acacgcgttg ccgagctcaa
     4321 agacggaaaa atcagcgaat acgacatccg cccagaagat ttcggtatcg aaacccgccg
     4381 caatttggat gaaatcaaag ttgccaatac tcaggaatct ttgttgaaaa tgaatgaggt
     4441 gctggaagga agagaagggg ctgcgcgcga tatcgtattg ctcaacaccg ccgccgccct
     4501 gtatgccgga aatgtcgctg cttcgctttc agacggcata tctgccgcac gggaagccat
     4561 cgattcaggc agggccaaat cgaaaaaaga ggagtttgtc ggttttcaac cacaacaaag
     4621 atgccatttt cttggaaaga tggagcttgg gtgatgccgc catgattatg gaacttttgt
     4681 ggcaaaacat aagcacttca cgaagagaac ttaccaaact gtttttatat aaaaacttgg
     4741 ggctgtacta gataaccaga ccaaattccc attaactaat tgtcttaaaa tctgaatttg
     4801 agattctatt taaaatgcca ttggcatttc tttaaatgca gccccaaatg ctctttggga
     4861 atgccgttaa acttacgtaa atggctaaat tcactaatat caagcacatc ataactacga
     4921 aaggtatccg tgtatacaat gccatcaggc ttaactttct ttcggataat tggcaacaat
     4981 gtcgctgatt gcgcattagg aacaacgacg gta
//
LOCUS       DAWKST010000144         4912 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.144, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000144 DAWKST010000000
VERSION     DAWKST010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.144"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(614..1150)
                     /locus_tag="WJL87_001687"
     CDS             complement(614..1150)
                     /locus_tag="WJL87_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ7800096.1"
                     /translation="MDLREVKLGGETIYEGGFVSISKDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDEGKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(1184..1540)
                     /gene="folB"
                     /locus_tag="WJL87_001688"
     CDS             complement(1184..1540)
                     /gene="folB"
                     /locus_tag="WJL87_001688"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ7800097.1"
                     /translation="MDKIFLHGMKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            1596..2198
                     /gene="plsY"
                     /locus_tag="WJL87_001689"
     CDS             1596..2198
                     /gene="plsY"
                     /locus_tag="WJL87_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ7800098.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAVLTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHLSWIWATLLIALLVLFRHKSNISNLLKGKEGKIGEKR"
     gene            2286..3131
                     /locus_tag="WJL87_001690"
     CDS             2286..3131
                     /locus_tag="WJL87_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ7800099.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAKNLPTELNGFIAHIQKILPSPKLYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKELISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            3377..4351
                     /locus_tag="WJL87_001691"
     CDS             3377..4351
                     /locus_tag="WJL87_001691"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ7800100.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPSKPGKLRLMYEANPMA
                     LILEQAGASASNARQDILGIRPESLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(4510..4728)
                     /locus_tag="WJL87_001692"
     CDS             complement(4510..4728)
                     /locus_tag="WJL87_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ7800101.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT         1273 a         1435 c         1016 g         1188 t
ORIGIN      
        1 aagtatccat atattctatc tttaattatt tcattatttt cttcattttc ttcatctaaa
       61 aatatacatc tttttaatag ttccccatta aattcataaa catcgtaata agctttaaaa
      121 atagatatca tatcatttcc ttatcttcca taaatcggct tcggttgttt aaataccgcc
      181 cgtgccaatt accgttctca aattcaaatt gggcttttag gtctataaca aagaaaaagc
      241 cttggtagga taaacctacc aaggcttttt cttaaaaaaa cgctctatta ccgtgcctaa
      301 aaatgcaaaa aagtaaccgt cataaccctt ttgccaaaat caatatcatc atccgatgaa
      361 tgaattgacg gtatataaga aagaattaat aagctcataa accggtaaga aaaatcagta
      421 acaaagatat tcattattaa ccattcttaa aaatgatcat gttcgtttgg gtaattatca
      481 tacccttttc cctcttatta ctaaagcacg aatgacttta aaggacgcgc ccagagacaa
      541 gccgaaaagg catatcccat ccgccaaaca aaatgcccgc cgacttactc cggcgggcag
      601 ctccaacacc ctgtcaatcc ttcatcagcc agtattgcaa accgattaat gtcttgccgt
      661 ctttaatttc atcgtttgcc aatgcctgac ggacttcctc cttcgacatc aatacggttt
      721 ccgtaatctc gtcttcgtca ttggcaagcg tactgcccaa gcgtacgcct tccgcctcga
      781 acagatacat tttttcgttg caaaaaccga ccgccgtata aaaactgtac aacaggcgta
      841 cactgtcggc aacataaggc gtttcctccg ccaattctcg cagcgcacac gctgccatat
      901 cctcgcccgc cacatccagc ttgcccgcag gaagttccaa tgtcgcctga tttgccgcat
      961 accgccactg ccgtaccaaa accactttcc cctcgtccgt aaccgccaac acgcatgccg
     1021 caccgggatg acggatgacg atacgctgcc cttcgttacc gttgggcaac ctgaccttat
     1081 ccttgctgat gctgacgaaa ccgccctcgt aaatggtttc gccgcctaat tttacttccc
     1141 tcaaatccac ttcatatctc ctttccgttt cctattctgc cgttcaatct tcacgcttgc
     1201 cgcgctcgat ttccacgcca acctcgcgca cgccttccaa aatacccggc ttgacgattt
     1261 tcacgcgcac ccacaccgcg ccgaaatatc ccaaaaccaa atcggcaata tattccgcca
     1321 acgcttccaa aagcaggaaa tcctgttctt tcagatgtcg gcgcagcgtt tcgcatacct
     1381 cggcataatg caccgtattg gcaatatcgt cgtccgaacc cgctttctcg ggaacgccga
     1441 tgtccaaatc gacaatcagg gtctgcaacc gttcgcgttc ccagccgtac acgccgataa
     1501 gcgtatctgc cttcatgccg tgcaaaaaga ttttatccat aatccgcccc gttttttgct
     1561 aaaataaccg caccattcta agtaaagtac cgcaaatgtt caacataccg gctgttgctg
     1621 tttcctatct gatcggctca ctttctttcg ccgtcatcgt gtccaagtat tacggcatgg
     1681 acgacccgcg cacctacgga tcgggcaatc cgggggcaac caatgtttta cgcagcggca
     1741 aaaaaaaggc ggccgtgctg acgctcttgg gcgatgccgc caaaggtttg gttgccgttt
     1801 tgcttgcacg cgtgcttcaa gaaccgctcg gtttatccga cagcgcaatc gctgccgtcg
     1861 cactcgccgc gctggtcggg catatgtggc cggtgttttt cggatttaag ggcggcaaag
     1921 gcgtggcaac ggcattgggc gtgcttttgg cactctctcc cgcaactgcc ttggtctgcg
     1981 cgttgatttg gcttgttatg gcattcggct tcaaggtgtc ctcccttgcc gcattaaccg
     2041 ccacaatcgc cgcccccctt gctgcgctgt tttttatgcc ccacctttct tggatttggg
     2101 cgacgctcct tatcgccctg ctggtgttgt tccgccacaa aagcaatatc agcaacctgc
     2161 ttaaaggcaa agaaggcaaa atcggcgaaa aacgctgatt cggcatttgt tttccattac
     2221 gtcatgccgt ctgaaaccat atttccgcgt tcagacggca tttccgcaca aacggctttc
     2281 ataccatgct cagttacaga cacgcattcc acgccggcaa ccacgccgat atgctcaaac
     2341 acttcacctt gtttttagtg ctgcaatatt tcaaccgcaa agacaagccc tactggtaca
     2401 tcgacacgca cggcggcgcg ggtgtgtaca atctcgaagg cagcgaggca caaaaagtcg
     2461 gcgaataccg gcaaggcatc gccctacttc gacaagcaaa aaacctgcca accgagttaa
     2521 acggctttat cgcgcacata caaaaaatcc tgccctcgcc caaactttac tgcggttctc
     2581 cgtggctggc gcaatcgctg acccgtgtcg gcgacaaatt gcgcctgttt gagctgcacc
     2641 ccaccgactt tgtccatctg caaaacaata tgggcgaagc aggattgggc aaacgcggac
     2701 aagtgttgcg cgaagacggt tacaaagagc tgatttccct gctgccgccc ccgccgcgcc
     2761 gcgccaccgt cctcatcgac ccgccctatg aggaaaaaca ggactaccgg cgtgtaacgg
     2821 aaacgctgaa agccgcttta aaacggtttg agtccggctg ttatctcatt tggtatccct
     2881 gcctcagccg cgaagaaagc cgcaagctgc ccgaagaatt gaaaaaactc ctgcccgaca
     2941 actaccttca cgccgaactt cacgtccacg cgccgaaagc cgacggtttc ggtatgcacg
     3001 gcagcggaat gttcgtcatc aatccgccct atctgttggc ggaacaattg gcggcaaacc
     3061 tccccgccct gacgcggctg ctggcgcaag acgaaggcgc gcgctacctt ttggacagca
     3121 aaatccgctg accccgatgc cgtctgaaac ccttcagaca acatctcccg tcaaataaac
     3181 aaaaccggac gatataccgg cagtcggcac ttttaagaat aaatatcccc tcatccgcct
     3241 gccgcgttcc gacttgcccc atgccgtttt tttacactac aatcccctag actatcctgc
     3301 aaaacgcacc aaaggaatac gccacaataa cctgccgcct ttcagacggc acaaaccttt
     3361 ccgaaacgag caaactatgg acacactgac ccgattcctc cccgaacacc tgcaacaaaa
     3421 ccagctgccc gaagcactcg gcggcgtttt actgtccgtt gtttccgcct gcaccgaaat
     3481 caacgccaaa gtccgcctcg gcgcactggc cggcgtattg ggtatggcgg gcacgggcaa
     3541 tattcagggc gaagaccaaa aaaaactgga tgttatcgcc aacaacatca tgattgacac
     3601 actcaaagcc aaccctgccg ttgccggttt ggcaagcgag gaagaagact ctttcgtaag
     3661 tgccggtgaa aacggacgct atctcgtcct attcgaccct ttagacggat cgtccaatat
     3721 tgatgtcaac atttccgtcg gtacaatttt ctccatcctt gaaaaacccg aaggcgcatt
     3781 ggcaaccgaa tcattccttc aaacgggcag acagcagctt gccgctggtt acgttcttta
     3841 cgggcctcaa acccagctcg tattcacatt cggacacggc gtatatatgt tcacgctcaa
     3901 tgccgaaaac gaatttgtgc tgacaaaaga aaaaccgaaa gtacccgaaa gtaccaaaga
     3961 atttgccatc aatatgtcca accgccgcca ctggctgccc cccgtccaac aatacatcga
     4021 cgagctcttg gcgggcgaaa ccggcacgcg cggcaaaaac tacaatatgc gctgggtagc
     4081 cagcatggtt gccgaaatcc accgcatact gatgcgcggg ggcgtgttca tgtatccgca
     4141 agacaaacgc gacccgtcca aacccggcaa actgcgcctg atgtatgaag ccaatcctat
     4201 ggccctgata ctcgaacaag ccggcgcatc ggcaagcaac gcccgtcagg acatactcgg
     4261 catccgtccc gaaagcctgc accagcgcgt cgccgtcatt atgggcagca gcgaagaagt
     4321 ggattacctc aaccgcctgc attcaaaata aaaggcagcc tgaccgataa agaaaggcgg
     4381 cagcgcgcat ccgacgctgc cgccgatatt tcgtttatag ccttaaaaag atagaaaacc
     4441 acctttccga acggcaaaaa tgccgtctga aaagttttca gacggcattt taaaaattaa
     4501 agcccgaatt tatttgcccg ctgctttttg caggatggcg tacaactcgt ctttcagttt
     4561 caatttggct tttttcagct catcgatggt ttccgcaccg ctggtaaccg gattgttgac
     4621 cagaccggta attttatcgt ccagctcgtt gtgttcgtcg aacagacggg cgaagcggga
     4681 attttcctgt ttcaatttgg aaatcaaatc acggtattct gggaacatat cgctttcctc
     4741 tcttggttta cggttaaaaa gtcaaactga atctttacat taattataac aaagataata
     4801 tttttatcca gtgttttatc ggatacggca aaattttctt ttaacaccgc cgtccggttg
     4861 ataaaaacgt tgacaatatc acgctttacg cataaatttt cagcctttcc ta
//
LOCUS       DAWKST010000145         4857 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.145, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000145 DAWKST010000000
VERSION     DAWKST010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.145"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(210..932)
                     /gene="rmpM"
                     /locus_tag="WJL87_001693"
     CDS             complement(210..932)
                     /gene="rmpM"
                     /locus_tag="WJL87_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ7800102.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPVVVVEQAPQYVDETISLSAKTL
                     FGFDKDSLRAEAQDNLKVLAQRLGQTNIQSVRVEGHTDFMGSDKYNQALSERRAYVVA
                     NNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPDRR
                     VDVKIRSIVTRQVVPAHNHHQH"
     gene            1296..2246
                     /locus_tag="WJL87_001694"
     CDS             1296..2246
                     /locus_tag="WJL87_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ7800103.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2359..3093)
                     /gene="fnr"
                     /locus_tag="WJL87_001695"
     CDS             complement(2359..3093)
                     /gene="fnr"
                     /locus_tag="WJL87_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ7800104.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTYFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3298..4719
                     /gene="hemN"
                     /locus_tag="WJL87_001696"
     CDS             3298..4719
                     /gene="hemN"
                     /locus_tag="WJL87_001696"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ7800105.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTASV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLVGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
BASE COUNT         1241 a         1329 c         1173 g         1114 t
ORIGIN      
        1 gcggcaatgg gacacaaacc tgaatctgaa tccgaacctg caccccgatt ctgacgatag
       61 ccgtttcaga aaaccaccca aaaaaacaaa ccccgcaaaa gcgggggttt gtttcacatc
      121 atcgatgccg accgacgttt cacgaatatt gcaacagtac ctggtacaaa atccactaac
      181 ctcatgcatc ggcaagatat tgcctagcct tagtgttggt gatgattgtg tgccggcaca
      241 acctgacggg ttacgatgct gcggattttc acatccacac ggcggtcagg ttcgatacat
      301 gcaatcagag cctcacgttt tttggcttta gagactttcg cacccagttt ggcaacttcg
      361 gcttcacaaa cttgagtcat ttgcgcttga gattcgccca agccgacagc agaaattcta
      421 gaaacaggta cgccgttgct gaccaggttg tttgccacta cgtatgcgcg gcgttcggac
      481 agggcctgat tgtatttgtc agaacccata aagtcggtat ggccttcgac gcggacagat
      541 tggatattgg tttgacccag gcgttgcgcc aatactttca ggttgtcttg agcttcggcg
      601 cgcaatgaat ccttatcgaa accgaacagg gttttggcag acagggaaat ggtttcatca
      661 acatattgcg gagcctgctc cacaacgacg acaggagcgg gttcgggttc tggctcgggt
      721 tcgggggcag caaccgcatc gccgcattct acgcgacctt ggcttgcttt atcaaagtag
      781 gcgtttttcc agcattcgcc atagttgttg cgtacgattt cgttcgactg gccgcttacg
      841 gtgtaaccct gaacggacgc ctcgcccgca acagcagtgc cggaagcgag caatgcaacg
      901 aataatgcgc ttaatttcag ctgtttggtc attttattcc ctcattaaat ttgtacagca
      961 gatacagaag caactgctga attgccaaca tacaggccgc tgtagaaaca tggcggcaaa
     1021 tcggatattt cagtctccac tataaagaag cagggggagg actgtcaata ctcaacgcct
     1081 gaaaacaacc gtaaaacagc ctgtttcctg atagtctatc atgccgaaaa atcaattccg
     1141 tgtcattact ttagggggat tttttccata tcgcacgatt gccgttgcac aaaaacaaca
     1201 aacacaacgg caaagcccca taccgtaccg gcaagaaaat atgataaatt ataaacaatg
     1261 ttcagccacc cgacacagac ccacaccgac ccgccatgaa attacaacaa ttgaaatacg
     1321 ccttagaagt ttaccagcac aacctgaacg tttccgaagc ggccgaagcc ttgtttactt
     1381 cgcaacccgg catctccaaa caaatcaaat tgctggaaga agaaatcggc attcagattt
     1441 ttatccgcag cggcaagcgc gtggtttcgg tctcgcagcc gggcaaggtg gttttggata
     1501 ttgcggaacg tattttacgc gatgtacaga acattaaaaa tatcggcagc gagtttaccg
     1561 gacaggacag cggttcgctg acggttgcca cgacgcatac tcaggcacgc tatgccctac
     1621 ccttgattgt tgccgatttt gtgaaacgct atccgaaagt caacctgacc atcaaacagg
     1681 ggagccctgc cgccatcgca cgtatggtta cctcaggcga ggcggacttg gcgattgtta
     1741 cggaacggat agacgaccat cccgaactgg gaaaacttcc ctgctatgac tggactcatg
     1801 cggttatcgt accgaacgac caccccttgc tcgaatgcag aaaccccctc cgcattgaag
     1861 atttggcgag gtttccgctg attacttatg aatttgcatt caatgcgggc agcagcatcg
     1921 cgcgggcatt ttccaaagcc cgtttggaac agccggatgt cgcattggcg gcggcggata
     1981 cggacgtatt gaagacttat gtgcgcttgg gtttgggcgt gggactgatg gcgaaaatgg
     2041 cgtacaaccc ggatacggac ggcgatttgc agcttgtgga tgcggcacac ctgttcgagc
     2101 cgtcgccgac gtggattgct ttgcgcagcg atacttattt gcgcggatat gcctacgact
     2161 ttatccaagc gtttgcgccg cacctgacac gcgagaaggt ggataggatt ctgtacacgc
     2221 ccatcagcga ggatttttcg atttaggcgg ctgccggttt tcagacggca ctttgcggca
     2281 gatacaacaa acaggacaga tgttttcgtc tgccctgtgt ttattgagaa tgccgtctga
     2341 aatgttcgta cgggttaatc aaatggcgtg cgaacaaccg gataccatct tcttcaacac
     2401 ctgcagattg aggatcttga tgtgtttgtg ttcgacggaa atcaatcctt cctgatgaaa
     2461 tttagataat gtgcggctga cggtttcaag tttcagcccg agataactgc cgatttcttc
     2521 gcgggacatt cttaagatga agtcgttggc agcaaaacct cgggaataaa ggcgttggga
     2581 aaggttcaac agaaaggcgg caatccgctc ttcggcgcgc atattgccca acagcagcat
     2641 aacaccttgg tcgcgcacga tttcacgact catcatgcgg aaaaagtagg tacgcaggct
     2701 ggggatattc tgccccagtt cttcgatgtg ggtaaacggc agttcgcaca cttcgctgtc
     2761 ttccaaggcg accgcgtcgc aactgtgcac atgggaacag atgccgtcca tgccgatgag
     2821 ttcgcccgac ataaagaaac ccgttacctg atcgcggccg tcctgactgg cgacggttgt
     2881 tttgaagaag cccgaacgga tggcaaagag cgaggtaaag gcttcgccga cacagaacag
     2941 gtattcgccc tttttcaggc ggcggctttg acggatgacg gcatcgagtt ggctgagctc
     3001 gttgggcagc agcccgacag gcaggcagag ttcccgcaaa gaacaggaag aacacagcgt
     3061 tttcatctga tgtgtagtat tatgcgaagc cataccgtac ctttttgtgc gctttgcccc
     3121 atcatgatta tagtggatta aatttaaacc agtacggcgt tgcctcgcct tgtccttatt
     3181 taaatttaat ccactatatg tgcttattga cacatatcaa gacaggttta tcatactgtg
     3241 gcattctacc aaactatcca aaatttgact aacatcataa aaccgcacac acacaccatg
     3301 aaaatcattc agatacagaa caatcacaat gtaaacgatg accgccccga gtttgaccgc
     3361 gcgctgattg ccagcctgcc cgccagcggc ccgcgctaca cttcctaccc taccgccgac
     3421 cgtttccatg acggtttccg cgaaggcgaa tatatcaaag ctttacattt gcgcggtatg
     3481 ggcgcgttaa acaaaccgct ttccctttac attcacattc cgttctgcaa caccatctgc
     3541 tactactgcg gctgcaacaa aatcatcacc aaagacaaaa gccgcgccga tgcctacatc
     3601 gaatatcttg aaaaagaaat ggaactgctc gctccacatc tgaacggacg gcaccagctt
     3661 gcccaactgc acttcggcgg cggcacgccg acctttttga gcgacgaaca gatcgaacgt
     3721 gtcttccgca tgatacgcaa acatttcgag ttaatcccca ccggcgaata ctccatcgaa
     3781 atcgacccgc gcaaagtcag ccgcgacacc gtcctcatgc tcggcagact cggcttcaac
     3841 cgcatgagca tcggcattca ggatttcgac cccaaagtgc aggcagcggt caaccgcatc
     3901 caaagttacg aagaaaccaa agaagtcatc gatgcggcgc gcgaagcggg gttcaaatcc
     3961 gtcagcgtcg atttgattta cggcctgccg caccagactt cggaaagcat caaaaccacc
     4021 atcgataccg ttttgtcgct cgatcccgac cgcctcgccc tttatcacta cgcccacctg
     4081 ccgcacgtgt tcaaaccgca acgccgcatc gataccgcct ccgtccccga cagcgaagag
     4141 aagctcgata tgctgcaata ctgcgtccaa accctaaccg aacgcggcta cgtcttcatc
     4201 ggcatggatc atttcgccaa acctgacgac gaactctcca tcgccctcaa agaaggcttc
     4261 ctccagcgca acttccaagg ctattcgacc tacgcggatt gcgatttggt cgccatcggc
     4321 gtgtcgtcca tcggcaaaat cggcagcacc tattcccaaa acgaacgcga catcgatgcc
     4381 tactatgccg ccatcgacga aggcagactg cccatcatgc gcggttatca gctcaatcag
     4441 gacgacatcc tgcgccgcaa tatcattcag gatttgatgt gccgcttcgc gctcgactac
     4501 cggatttacg aaagtatgtt cggcatcccg ttcgaccgct acttcaaaga cgaactggcg
     4561 gatttggaaa aactcgtcgg tttgggattg gtgcgcctga acagccacgg gctgaccgtt
     4621 accccgaaag gacgcttcct catccgcaac atcgccatgg tgttcgacta ccacctgcgc
     4681 cacaaggaaa ccaaggcgaa atattcgcaa acggtgtgat tgcgtctaac gtacaaatgc
     4741 cgtctgaaag gctttttcag acggcatttt gctgccggca ggataagtgt tttcaagaac
     4801 aggcggcggc atatcataac gttccgcacc tttgtgtccg accgttccga aaccaag
//
LOCUS       DAWKST010000146         4847 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.146, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000146 DAWKST010000000
VERSION     DAWKST010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.146"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(113..325)
                     /locus_tag="WJL87_001697"
     CDS             complement(113..325)
                     /locus_tag="WJL87_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7800106.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWIEKTNAV"
     gene            complement(337..618)
                     /locus_tag="WJL87_001698"
     CDS             complement(337..618)
                     /locus_tag="WJL87_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800107.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(655..918)
                     /locus_tag="WJL87_001699"
     CDS             complement(655..918)
                     /locus_tag="WJL87_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800108.1"
                     /translation="MADILPSDKRRQPPKTKGNPMNIIEQEMKHSSLRKVDAEIAKII
                     ADAHKINAESVKIAQESRWYPMIAATGLVTAIAAVLALIFKFA"
     gene            complement(1012..1563)
                     /locus_tag="WJL87_001700"
                     /pseudo
     CDS             complement(1012..1563)
                     /locus_tag="WJL87_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1542..1853
                     /locus_tag="WJL87_001701"
     CDS             1542..1853
                     /locus_tag="WJL87_001701"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800109.1"
                     /translation="MTSPVSPSIRAASIFPLARSISTACRTDINRLPLRLIASTKTAE
                     INLASSGLSSVLPASLRRLRVGLTLTASSLRYSVARLMPNSCACCLRYAERAPRSIAS
                     A"
     gene            complement(1875..2750)
                     /locus_tag="WJL87_001702"
     CDS             complement(1875..2750)
                     /locus_tag="WJL87_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ7800110.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELAEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHGISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(2795..3085)
                     /locus_tag="WJL87_001703"
     CDS             complement(2795..3085)
                     /locus_tag="WJL87_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ7800111.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(3097..3276)
                     /locus_tag="WJL87_001704"
     CDS             complement(3097..3276)
                     /locus_tag="WJL87_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ7800112.1"
                     /translation="MYGYTVEKLKENFAQNGQTLAQWARENGFKPRDVYLVVGGQRKG
                     NYGKGHEIAKKLGLK"
     gene            3390..3767
                     /locus_tag="WJL87_001705"
     CDS             3390..3767
                     /locus_tag="WJL87_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7800113.1"
                     /translation="MISKSLFGNRLKEKRKFLGLTQAQAAEKAGIERETWGKYERGVF
                     MPSGDVLLSFLNMGIDVSSLFAAEQGIRPSENSEISKEESELLGYYRQASDNGKFVIL
                     SVAKGAEKKAAENTQVSATHKTA"
     gene            3796..4041
                     /locus_tag="WJL87_001706"
     CDS             3796..4041
                     /locus_tag="WJL87_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800114.1"
                     /translation="MFDFEQQIKWGERAEEIVKEAATQNNIEIPEPLASALAKAVKVH
                     YLSQAGVFSLVEAYADTVNPTEKEVDYQAIGKELFEK"
     gene            4275..>4847
                     /locus_tag="WJL87_001707"
     CDS             4275..>4847
                     /locus_tag="WJL87_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800115.1"
                     /translation="MAKKTTKTIHYLRAQQNHSLFDLEVALRRVLSAAPTVQDTQIEQ
                     YNQLTQIMHRTLDPKDVKGNSIGGLLIHIGSGTKDEHIRTMSNRPVQQDDHGGTQAPP
                     SGYSFLRKEAFLYIVGHHVIFCGHGFLSASTVASYLSLLSNKLRENNPDIVQFNIEFK
                     AVGNCDKLSLIQQHGVKSILLDASAYQLSRN"
BASE COUNT         1072 a         1148 c         1256 g         1371 t
ORIGIN      
        1 tgagtttatt ttggggcggc ggcgggtcgg gggcaagcgg cgtggggctt ttaggtttgg
       61 gggtgggtaa aaaatgccgt acccggcggg caggcttcgc actctgaagg tttcagacgg
      121 cgtttgtttt ttctatccaa tcgaggaact gccgccattt ttccagcggc atatcggccc
      181 gacgggtttc ggtatcgggt tcggcttccc agcggcctac ctgtatgtag tgcttcaccc
      241 cgacgatttg cgccaactcg gcctgtgtca gtttgcagcg gttgcgaagg gtgcgaaggt
      301 tgtaaggcgt gtagccgagt tccatgtcgt tgcggtttat tttgtttcgc atattttttt
      361 gactgccctg cggcaggttt cggtaaggac ggcggcaaat cgggcttcgt ctgccggttt
      421 gccgtcgaaa aaaataaaat cgtaaagggt gatgccgttt tcggaatggc ggtaatgccc
      481 gcccgggcat tcgccgtctg tctgcctgtg gattttggca atcaggcggg gatagcggca
      541 atgggtaatg aaacggcttg cgccgtcttg cccgataatc cattcggggt agcggttgaa
      601 tagtgcggct ctgttcattt tgttgaggga taaagcccct cgcggggctt gtggtcaggc
      661 aaatttgaat atcagtgcca acacggcggc gatggcggta accagcccgg tggccgctat
      721 catgggatac cagcgggact cttgggctat ttttacggat tcggcgttga ttttgtgcgc
      781 gtctgcaatg attttggcga tttcggcatc tacttttctc agactggagt gtttcatttc
      841 ttgttcgatt atgttcattg gatttccttt cgtttttggc ggttgccgcc gcttgtcgga
      901 tggcaggatg tctgccatgt gtgtacatta atacctttta ggttttattg caagtgtttt
      961 gggcaggatg ccgtctgaat ttttttcagg cggttttttg ttttgcgggg ttttggcggc
     1021 ggcttcgtat gcttggcggt ggcggcggct gtaccattcg gcgagttcgc gccgctcttg
     1081 gaggcggtag ctgtcggcgg tcaggtagtg gtgcaggctt gagaagccgg cgcgttggag
     1141 ggcggcgcgg ctgatgtggc cgagggcgag gtctgttgtg agggtgtctt ttccggcggt
     1201 caggctgatg ttggtgaaga ggtggcggaa tccgtgcatt gtgtgtttgg atttgccggg
     1261 ggtgctgccg tcatagccca gtcgtcggat ggcgttgtgg gcgaatttga tgctgatgtg
     1321 gtcgggatgg ggggcgggtt tgcgccgtgg gcggatgcct gggaaaaggt ggatgttgtc
     1381 gccggtctgt gtgtgcagct ctcggagtat ttctaccgcc cagtccgaca gtgggacggt
     1441 aaaggggtgt ttggtcttca tgtcggcggc ggggatgtgc cataaccggg cggtgaggtc
     1501 gatgtcttgc cagcgggcgg aaagcagtgc ggacggacgg gatgacttcc ccggtttccc
     1561 catcaatccg cgccgcctcg atttttccgt tggcgcgcag catttccacc gcctgccgca
     1621 ccgacattaa ccgcttgccg ttgcgcctca tcgcctccac caaaaccgcc gaaatcaatt
     1681 tggcttcttc cggtttaagc tccgtcttgc ccgcatcgct gcgccgtttg cgcgtcggct
     1741 tgacgctgac cgcctccagc ttgcggtata gcgtggcaag gctgatgccc aattcctgcg
     1801 cctgctgctt aagatatgca gagcgtgcgc cgcgttccat tgcttccgcc tgattctcga
     1861 ctgccttaag acgctcaatc attgccggat tcatcgcctt ctcccgtttc accgcccaac
     1921 cattccggca cattgtctgt cggtgcttca gtcggcaggg catagctttc gcgcagttgc
     1981 tcgcagtcca aaataatttg attgagcgtg ccgaccatct ttgcctgatg gctgatcccg
     2041 tgtgcctcac tgtgcgcatt aagttggtcg aacaaatctt tcagacggct cacttgactg
     2101 cggataccga cctcaaggct tgttaactgc atcgccaact cgctgcctac gtcttccgcc
     2161 ttcggctctc tgacaacggt ttgcttctta gccagcttct cggccagctc atcaattttg
     2221 gctgttttgg ttttcatcac ttcgtctttg gcggcgaggt tttcgcggct ttcgcgcagg
     2281 gcgacgcgca gctcgcgcac cgtcattcgg tccacatcgt caaaggtcat gccgttgact
     2341 tcttcccctt cggccaaacc caccagcgta acgtcttctt cgaccagcag ctcaagcagc
     2401 ttcgacttgc ccaaatccat cagcttcggc gcggctttct gcatttgcgg ggtcgcaaag
     2461 cggcgagtgg ctgacatcag acgtgatgtt tctgcgatac ccagcccaaa ttggcttttc
     2521 acaatttcca taaaccgtcc gtgttccgta tgctctttta aaataatcag cgcacgtccc
     2581 agttcgaaca tgccttccat cgtctgccgt actgcttgac gaccgcgttc aacccaacgc
     2641 tcttcgctat aagtctcgcc gttaccccac tgctccatca ccagcacact gcgcatcgct
     2701 gcctgattgc ttactttgtc ggttgcgata atttcaattt ctgtattcat tttttatctc
     2761 caaagtttcc gacgtcggaa actttcaaaa tccgttaatc cacatcgacc cgcttgccga
     2821 tttcggcaat cttgctttgc agccgttcat gctgctgcct gaaccgctct gcgatttgca
     2881 gggttttgat gccgtaggcg tagttgccgt tttcaagttt gatgaccaat cccgaggcaa
     2941 ccaaatcatc aatatccctg ctgacttgcg atggcgtcag ccccagtccg accgataaat
     3001 ccttattgct cagaccgata atcggatgct cgtcaagcgc gataaagacc ctcaatagcc
     3061 gttgtaccct tttactttct gccatccgca tcctccttat ttcagtccca gcttcttggc
     3121 aatttcatgc cccttgccgt aattgccttt gcgctgtccg ccgaccacca gatacacatc
     3181 gcgcggctta aagccgttct ctcttgccca ctgcgccagc gtttggccgt tttgggcaaa
     3241 attttctttt agtttttcaa cagtatagcc atagatagcc ctttcccatt gtgataaaat
     3301 agtgaactat ttatgcacta gaaattaatt agtgcattac caatgcgtga attatgagat
     3361 tttaaaatct cttttgcaag gattattttg tgatttctaa atctcttttt ggtaatcgtt
     3421 tgaaagaaaa aagaaagttt ttagggctta cccaagcaca agctgctgaa aaagcaggta
     3481 ttgagcgcga aacatggggc aaatacgaac gtggcgtatt tatgcctagt ggagatgtat
     3541 tgctctcttt tttgaatatg ggaatagatg tgagcagctt atttgctgct gaacagggaa
     3601 ttaggccgtc tgaaaattct gaaattagta aggaagagtc agagttatta gggtattacc
     3661 gccaagccag cgataacggc aaatttgtca ttctcagtgt ggcaaaaggc gcagagaaaa
     3721 aagcagccga aaatactcaa gtttcagcca cgcataaaac agcgtgattt ttaaatccaa
     3781 aatggagaca gtcttatgtt tgactttgaa caacaaatca aatggggtga gcgagcggaa
     3841 gaaatcgtta aagaggcagc cacccagaac aatatcgaaa ttcccgaacc tttggcttcg
     3901 gcattagcta aggcggtaaa agtgcattat ctaagccaag ctggtgtgtt ttctttggta
     3961 gaagcctatg cagacacggt aaatccaact gaaaaagaag tagattacca agcaatcggc
     4021 aaagagctat ttgaaaaata aaaaatgccg tctgaaattt tcagacggca tttaggacac
     4081 tccgacagtc gggctgctcc ggtgttctga aacggatatt aagcaattga agcggccgtg
     4141 cattttaaaa cgcattaaaa actaccccga catacaggtc atcgggcagg tggttcaggt
     4201 gttgaaagat ttgaattgat gccgtctgaa tttatttttt cgacacagat attaaaagag
     4261 aaaggactgt acaaatggca aaaaaaacta ctaaaactat tcattattta cgtgcgcagc
     4321 aaaaccatag cttgttcgat ttagaggtag cattaagaag ggttttgtca gctgctccta
     4381 ctgttcaaga tacacaaatt gaacaataca atcagcttac ccaaatcatg catagaactt
     4441 tggatcctaa agatgtaaaa ggcaattcaa ttggtggatt actgatacac attggcagcg
     4501 gtacaaaaga tgaacatatc cgcactatga gcaatagacc tgtacagcaa gatgatcatg
     4561 gtggaacaca agctcctcca agtggatatt cctttttgcg taaagaggct tttttgtata
     4621 ttgttggaca ccatgtaatt ttttgcggac atggtttttt gtcagcatca acggttgctt
     4681 cttatttaag tttacttagc aataaattga gagagaacaa tccggatatt gttcaattta
     4741 atattgaatt caaagcagta ggcaattgcg acaagttgtc attgatacaa caacatgggg
     4801 taaaaagtat tcttttagat gcttcggcat atcaattatc cagaaac
//
LOCUS       DAWKST010000147         4836 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.147, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000147 DAWKST010000000
VERSION     DAWKST010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.147"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            95..901
                     /locus_tag="WJL87_001708"
     CDS             95..901
                     /locus_tag="WJL87_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ7800116.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
     gene            complement(959..2224)
                     /locus_tag="WJL87_001709"
     CDS             complement(959..2224)
                     /locus_tag="WJL87_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ7800117.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDGTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGVTLNPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEAIIKEFQTKGFITNE
                     AGYGKEITNAELLALEVDVLAPCALENQLTSENAGKVRATIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            2631..3341
                     /locus_tag="WJL87_001710"
     CDS             2631..3341
                     /locus_tag="WJL87_001710"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ7800118.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPKTE
                     AGLALLKSLGIPLAVVTNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKDEE"
     gene            3414..3875
                     /gene="recX"
                     /locus_tag="WJL87_001711"
     CDS             3414..3875
                     /gene="recX"
                     /locus_tag="WJL87_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ7800119.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IDVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            3949..4110
                     /locus_tag="WJL87_001712"
     CDS             3949..4110
                     /locus_tag="WJL87_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800120.1"
                     /translation="MSEQPEKNHNPLLEDERKNPVYRMGQAVAGFMLVVWVAVLALVF
                     FLVFRFWLS"
     gene            4263..4745
                     /locus_tag="WJL87_001713"
     CDS             4263..4745
                     /locus_tag="WJL87_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ7800121.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
BASE COUNT         1271 a         1417 c         1101 g         1047 t
ORIGIN      
        1 agacagtttt cagacagcaa atccgtcttc acacgatacc tattttgtta taacataaca
       61 aaatctttga cccacacgag acaaaggctg caccatgaag aaaacattga cactgctcgc
      121 cgtttccgcc ctatttgcca catccgccca cgcccaccgc gtctgggtcg aaaccgccca
      181 cacgcacggc ggcgaatacc ttaaagccga cttgggctac ggcgaatttc ccgaactcga
      241 acccatcgcc aaagaccgcc tgcacatctt cagcaaaccg atgcagctgg ttaccgaaaa
      301 aggcaaggaa aacatgattc aacgcggcac atacaactac cagtaccgaa gcaaccgtcc
      361 cgttaaggac ggcagttacc tcgtcatcgc cgaatatcag cctactttct ggtcaaaaaa
      421 caaagcaggc tggaaacagg cgggcatcaa agaaatgcct gacgcaagct attgcgaaca
      481 aacccgaatg ttcggcaaaa acatcgtcaa cgtcggacac gaaagcgcgg acaccgccat
      541 catcaccaaa ccggtcggac aaaacttgga aatcgtcccg ctggacaatc ccgccaacat
      601 tcacgtaggc gaacgcttca aagtccgcgt tctgttccgt ggcgaaccgc tgcccaatgc
      661 caccgttacc gccacctttg acggcttcga caccagcgac cgcagcaaaa cgcacaaaac
      721 cgaagcacag gctttctccg acagcacaga cgacaaaggc gaagtggaca tcatcccctt
      781 gcgccaaggc ttctggaaag ccaatgtcga acacaaagcc gacttccccg atcaaagcgt
      841 gtgccaaaaa caggcgaact actcgacttt aaccttccaa atcggccatt cgcaccatta
      901 atcccgcccg cacaaaaatg ccgtctgaag gcttcagacg gcattttttg ttcaaacatc
      961 aataccaacc gcgcagtttc atcgcttttt caacacggcg gatactcatc atgtaagacg
     1021 cggttcgcaa atcgacatca tactcttgcg ccaagttcca tatatcgcgg aacgcacggc
     1081 gcaggacgac ggtttctttt tcctgaactt cgtcaaactc ccaataatag ccttgcagat
     1141 tttgtaccca ttcaaaataa gataccacca cgccgccgca gttcgccaaa atatcgggca
     1201 cgaccaatac gccgttttga cgcaggataa cgtcggcttc gggcgtagtc gggccgttcg
     1261 cgccttcgac cacgattgtc gcgcggactt taccggcatt ttcagaagtc agttggtttt
     1321 ccagtgcgca aggggcaagt acgtccactt ccagagccaa aagttcggcg ttggtaattt
     1381 ccttgccgta gccggcttca ttggtgatga aacctttggt ttggaattct ttgatgattg
     1441 cttccatatc caagccgttt tcgttgtaaa tggcaacgtc aacagtagaa accgcaacaa
     1501 ctttcgcgcc ggattgatgc gcgtaataac ctgtgtggta accgacatta ccgaagcctt
     1561 ggatggcgta agtggcaccc ttcacgtctt tgcccagttt ttccaaggct tggacggcgg
     1621 cgaggttcac gccgtaaccg gtagcctcgg tacgcgccaa agagccgccg aactcaaccg
     1681 gtttaccggt aaatacgccc ggtgcagaat gtttcaccac gttttcatag gcatcaacca
     1741 tccaagacat gattttgccg ttggtgttca catcaggtgc gggaatatcg attttctcgc
     1801 cgatcagcgg agaaatggct tcggaataag cgcgggcgat gcgttccagt tccgcctcgg
     1861 aataatcgcg cggattcaag gtaacgccgc ctttgccgcc gccgtaagga atacccgcaa
     1921 cgcagcattt gatggtcatc caaatcgaca gggctttgac ttcgtccaga ttcactccgg
     1981 gatggaagcg cacgccgcct ttataggggc cgacggcgtt gttgtgttgc gaacggtagc
     2041 ccgtgaaggt tttgaccgtg ccgtcgtcga gtttgacggg gaaattgact tccaacacgc
     2101 gggtcggact cttcaggatt tcataaacgg ccggatcggt tttcagccgg tcgcaggcgg
     2161 ttttgacctg tttgcgcgcg atttcaaacg gattgagggt ttcttttgca agggcttcag
     2221 acattttgct tccttttcac aaaaagattt tcaggggcgt gcatttcgct cattactata
     2281 accaatttcc cgacaaaatg taatagcgtg tagtcggcat aagcccgatt tgattaatct
     2341 atatatgatt ttatttttca agccgcacgg aatcagcccg gactccgcct gaaaaagcag
     2401 cgcgcgcgtc cgaaaaataa atgtcaaatt aaataaagat ataggcatcc ttttattttt
     2461 taaaaattta attggaacat cgctgggatt tcaaaccctt gcccgattcc gccccgaaat
     2521 ccgaaaacac cgcctgcaaa acctgttgcg attgttaaca atctatacat tagaagccct
     2581 gtgcaaacga tgttaaaata aaccctttca acccgacagg aaatcagatt atgaatgcag
     2641 ccatcgaaca cgtccaagcc gtcgccttcg atttggacgg cacactgtgc gattccgtcc
     2701 ccgaccttgc cgccgccgca gaagcgatgt tggagcaact cggtatgaaa ccgctgcctg
     2761 caaaagtggt cgaaagctat gtgggcgacg gcatcggcaa actggttcac cgcgtcctca
     2821 ccaacgaccg cgaccgcgaa gccgattccg aactgtggga aaaaggtttc gtatcctata
     2881 tgaaatacta ccgcgaccat ttgagcgtct tcacccgccc ctatcccaaa accgaagccg
     2941 ggctggcatt gctcaaatct ttaggcatcc cgctcgccgt cgttaccaac aaaaacgaaa
     3001 tccttgccgt cgaactgttg aaacagctcg gacttgccga ctacttcagc ctgatactcg
     3061 gcggcgacag cctgcccgag aaaaaaccca gccccctgcc gctgcggcat gccgccgaag
     3121 ttttgggtat cgatgttgca aacatggtta tggtcggcga ctcgcgcaac gacatcatcg
     3181 ccgccaaagc cgccggctgc ctgagcgtcg gcgttacctt cggttacggc gatatgacgc
     3241 tgctctcgca agacgatgcg acccgccccg actggattat cggctcgctg cccgaaattt
     3301 acgaaaacct gcaaccgcag aaaaataaag acgaagaata aaacaatctg ttcagacggc
     3361 atcggaatcc gtttcccatg ccgtctgaat ctttccgtgc cgaaaccgcc gccatgaaac
     3421 cgcaaaaatc cctacgcgcc cgcgcgatgg acatcctctc gcgccaagaa gtcagccgca
     3481 tcggtctgaa acgcaaactt gcaccgcacg ccgaaagcga agaagagttg gaaaacgtgt
     3541 taaacgaatt tgccgaacgc aactggcagt cggatttgcg ctatgccgaa gcctatatcc
     3601 gcagcaaaag ccgcaaacac ggttcattga ggctgaaaca ggctttggcg caacagggca
     3661 tagatgaaga aaccagccgc aacctgcttc ccgaccgctc aagcgaaaaa caggccgcca
     3721 tagacgtgtt gcgtaaaaaa ttcaaacacc ccgccgccga ccttaaagaa aaacaaaaac
     3781 aggcgcgttt cctcgcctat cgcggattcg atgccgatac cgttcagacg gcattgaaac
     3841 acgcttggga cgaaaattgg gaagacggct gctgaccttc cttcttgaat ctttttgcac
     3901 ggcaacataa ccttacgccc gtttccaact tttccgattg agaacaaaat gtccgaacaa
     3961 cccgaaaaaa atcataaccc cctccttgag gacgaacgca agaaccccgt gtaccgcatg
     4021 gggcaggcag ttgccggatt catgctcgtc gtttgggtcg ccgtattggc actcgtgttt
     4081 ttcctggtct tccgtttttg gctttcctaa acaaaatgcc gtctgaaggc ttcagacggc
     4141 atcggcggtc catttcggca gactatccca tcatagcttt ttttagcttg aattccactt
     4201 ccccattccc taaaatttcc ccgcacccat ttcaaaacac cctttcttaa aacaggtaca
     4261 ccatgacaca acaacgccaa ctgccttccc acgaactcat tatgtccgaa ctgatgatgc
     4321 cggacaccgc caatttcagc ggcaacgtac acggcggcga actcctgctc ctgctcgacc
     4381 aagtcgccta ttcctgcgcc agccgttaca gcggcaatta ttgcgttacc ctgtcggttg
     4441 acaaagtcct gtttaaagaa cccatccaca tcggcgattt ggttaccttt tacgccgctg
     4501 tcaactacac gggacgcacc tcgatggaaa tcggcatccg tgtcgaagca caaaacatcc
     4561 gcaccggcga aatccgccac accaacagct gctacttcac gatggttgcg gtcaaagacg
     4621 gcaaacccgt ccctgtccct ccgctggaaa tcctgaccga ccgccaacgc tgccgctacg
     4681 aaaaagccaa aaaacgcaga gacatcagcc tgcaagcctc tgaagacatg tcctgcggct
     4741 gctgacggcg gactatgccg tctgaaagac aggcacatcg cgccatccgt ttccattgca
     4801 aacggatgaa atcaagcaaa tatagtggat taaatt
//
LOCUS       DAWKST010000148         4835 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.148, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000148 DAWKST010000000
VERSION     DAWKST010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.148"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            245..982
                     /locus_tag="WJL87_001714"
     CDS             245..982
                     /locus_tag="WJL87_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEZ7800122.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
     gene            985..1671
                     /locus_tag="WJL87_001715"
     CDS             985..1671
                     /locus_tag="WJL87_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ7800123.1"
                     /translation="MAGLTFEQTVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPVAIVRKVLLNEARTGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            1830..2693
                     /locus_tag="WJL87_001716"
     CDS             1830..2693
                     /locus_tag="WJL87_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800124.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAEKKE
                     IVFGTTVGDFGDMVKEQIQPELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARALVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(2756..3325)
                     /locus_tag="WJL87_001717"
     CDS             complement(2756..3325)
                     /locus_tag="WJL87_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ7800125.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSTEWQGMAD"
     gene            3483..4343
                     /locus_tag="WJL87_001718"
     CDS             3483..4343
                     /locus_tag="WJL87_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ7800126.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGKIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            4479..4817
                     /locus_tag="WJL87_001719"
     CDS             4479..4817
                     /locus_tag="WJL87_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800127.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRSNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
BASE COUNT         1206 a         1450 c         1183 g          996 t
ORIGIN      
        1 tttcaattat ttgccgtttt ggtgcgaacc gctgcctttg cccgtttcag acggcattgt
       61 ccgaaatggt tgcctgcttc ctgccttatt gacaaaaaaa tgctttcccg ataatatcct
      121 acgaaaatta acctgccgat ttgacacagc ttgcgggcat aacagctaaa gcgttccgac
      181 aattccagct ttatcttccg cgcccgttgt gtccgacatc gggctttgtt gtatgggaaa
      241 gacaatgatt attttggaca aggtttccaa gcattaccaa acgcgcgaca agacccgttt
      301 tgccgctgtc gagccgacca gcctcgaaat ccgcgacggt gaaatcttcg ggctgatggg
      361 ttattcgggt gcaggcaaat ccaccctgtt gcgcctgatt aacctgttgg aacgtcccga
      421 cagcggcaag gtcaacgtct gcggacaaga gctgaccgcg ctcgatgccg ccgcattgcg
      481 tcaggctcgg cagaatatcg gcatggtgtt tcagcagttc aacctgttga acaaccgcac
      541 cgtcgccggc aacgtcgcct tcccattgga aatcgccgga tggccgtctg aaaaaatcaa
      601 agcgcgcgtt gccgaatgtc tcgaaatcgt cggactgacc gaacgcgccg gccactatcc
      661 cgcccagctt tcaggcgggc agaaacagcg tgtcggcatc gcccgcgcac tcgcgcccaa
      721 accccaagtc atcctcgccg acgaacccac ttccgccctc gaccccgcca ccacgcgcag
      781 cgtcttggaa tgtttggaag acatcaacaa acgattcaac gtaaccatcg tcatcgtaac
      841 ccacgaaatg agcgttatca gacgactgtg cgaccgcgcc gccctgttgg ataaaggcaa
      901 agtcgtcgaa atcgtcgaag tacgcggcaa ccaaatccac gcccgatccg acattgggcg
      961 cgaactgatt cgggaggact gaatatggca ggcttaacat ttgaacaaac cgtttccaac
     1021 atcatcggta tgaaaggcga aatcgtccgc gcattgggcg aaaccttcgt aatggtcggt
     1081 ttgtccacca cattcgccgt cgttttcggc acgttgctgg gcgtgttgct cttcgtaacc
     1141 tccagccgcc aactgcatta caacaagccg gtaaatttcc tgctcgacaa cctcgtcaac
     1201 ctcatgcgcg ccttcccctt cgtcattttg atgattgcga tgatacccgc cacacgcgcc
     1261 attgtcggca gcaccatcgg tccggttgcc gcctcgctgg tattgagcgt gtccggtctg
     1321 ttttacttcg cccgactggt ggaacaaaac ctgcgcgaag tccccaaagg cgtgattgaa
     1381 gccgccgccg cgatgggtgc gccgccggtt gccatcgtcc gcaaagtcct cttaaacgaa
     1441 gcgcgcacgg gcatggtttc cagcattacc gtgcttgcca tcgggctttt gtcatacagc
     1501 gcggcggcag ggatgatagg cggcggcggc ttgggcgacc tcgccatccg ctacggctac
     1561 taccgctacc aaaccgaagt catcgtcttc atcgtcgccc tcctcgtgct gctggtcatc
     1621 ctgattcaaa gcaccggcaa cgcgttggcg cggaaactcg acaaacgtta aacccgaatg
     1681 ccgtctgaac gccaaaaccc ccaccgctat ccgaaaaatg ctataaaatc cccctgttcg
     1741 cggcaaatgc cgtctgaacg ccgaatctgg acggcaggac tccctgcccg tcatttttgt
     1801 ttgaaactgc cacaacatca ggagaaaata tgaaaacctt cttcaaaacc ctttccgccg
     1861 ccgcactcgc gctcatcctc gccgcctgcg gcggtcaaaa agacagcgcg cccgccgcat
     1921 ccgcttctgc cgccgccgac aacggcgcgg agaaaaaaga aatcgtcttc ggcacgaccg
     1981 tcggcgactt cggcgatatg gtcaaagaac aaatccaacc cgagctggag aaaaaaggct
     2041 acaccgtcaa actggtcgag tttaccgact atgtgcgccc gaatctggca ttggctgagg
     2101 gcgagttgga catcaacgtc ttccaacaca aaccctatct tgacgacttc aaaaaagaac
     2161 acaatctgga catcaccgaa gtcttccaag tgccgaccgc gcctttggga ctgtacccgg
     2221 gcaagctgaa atcgctggaa gaagtcaaag acggcagcac cgtatccgcg cccaacgacc
     2281 cgtccaactt cgcacgcgcc ttagtgatgc tcgacgaact gggttggatc aaactcaaag
     2341 acggcatcaa tccgctgacc gcatccaaag cggacattgc cgaaaacctg aaaaacatca
     2401 aaatcgtcga gcttgaagcc gcgcaactgc cgcgcagccg tgccgacgtg gattttgccg
     2461 tcgtcaacgg caactacgcc ataagcagcg gcatgaagct gaccgaagcc ctgttccaag
     2521 aaccgagctt tgcctatgtc aactggtctg ccgtcaaaac cgccgacaaa gacagccaat
     2581 ggcttaaaga cgtaaccgag gcctataact ccgacgcgtt caaagcctac gcgcacaaac
     2641 gcttcgaggg ctacaaatcc cctgccgcat ggaatgaagg cgcagccaaa taaggcagtc
     2701 gtataaaatg atgccgtctg aaccgcattc cgtgttcaga cggcattttt gtcctttaat
     2761 ccgccattcc ctgccattcc gtcgaatccg gtgtatcgat tccgaacagc gacaaagcgt
     2821 gtgcaacact gtgcgccact atgtcgtccg ccgtctgcgg tttgcggtac atcgcaggaa
     2881 cagggggaaa caccacgccg cccatttccg ttacccgctt catattgtcc aaatgggcaa
     2941 ggttcagcgg cgtttcgcgc accatcagca ccagccgccg cctttccttc aaaaccacat
     3001 ccgccgcacg cgtcaagagg ttgtcgccga agccgtgcgc gaccgaggca agtgtccgca
     3061 tcgaacaggg ggcgaccagc atcccgtccg ttttaaacgt accgctggca atgcacgccc
     3121 cgatattgcc gatcggatgc acgaagtccg ccaaggcata tacctcatct ctcgcataag
     3181 ccgtttccga agcgcgcgcc atctccgcac ctttcgatac cacaaggtgc gtttcgatat
     3241 cttgcgcgcg taaaagttcc aaagccttca cgccgtattg gaaaccgctc gccccactga
     3301 tgccgattat caaacgccgt accatcatcc gcctttccca taaaaccgcc tgcaacggca
     3361 aaccggctat tatagtgaaa aaacagaaat ccgataaata cggatacaaa ttgtcggcaa
     3421 cacccaatat ccgataaaat atccgattta acatcctatc tgaataggca cgggagggcg
     3481 gtatggcaaa agtaaaaggc ggattggggc gcggtttgga ttcgctgctc gccaacggcg
     3541 cagacaacag cagcggcgac cgattgacca cggttgcggt taaagatatc cggcccggcc
     3601 gctatcaggc gcgtgttcaa atcgatgacg aagccttgca ggagctggcg gattcgatta
     3661 aggcgcaagg cgtgatacag cccgtcatcg tgcgcgaaca cggactgtcc cgatatgaac
     3721 tgattgcggg ggagcggcgt tggcgtgccg cacagattgc cggcctgacc gaaatccctg
     3781 ccgttatcaa aaccatcagc gacgaaaccg cattggcaat gggtttgatc gaaaacctcc
     3841 agcgcgaaaa cctcaacccc atcgaagaag cacaaggctt gaaacgcctt gccgacgagt
     3901 tcggactgac ccacgaaacc atcgcccaag ccgtcggtaa aagccgaagc gcgatttcca
     3961 acagcctgcg ccttttaagc ctgcccgaac ccgtgcagga aatgctttac caacgccgcc
     4021 tcgaaatggg gcacgcccgc gcactgctga ccctgcccgt cgtcgaacag ctcgaattgg
     4081 cgcaaaaggc cgtcaaaaac ggctggtcgg tgcgcgaagt cgaacgccgc agccaagccg
     4141 cccttcaaaa caaacgtccc gagcccaaaa agactgccgc cgccgacatc ggccgcctga
     4201 atgatttgct gactgaaaaa ctgggtgtca acgccgaaat caaaaccgcc aaccataaaa
     4261 aaggcaaaat catcctgcat ttcgacacgc ccgaaacctt cgaccatatc ttgaagcaat
     4321 tgggcataga ttaccgacct taattttgcg ggatataccg tctgaaatat agagaatagc
     4381 tttccagatt ttaagtggga aatataattc tattgacatt ttttctgctt cacgtaagaa
     4441 tcgttttcct gttttcattt ttaattttcg aggaaattat gaacacacgc atcatcgttt
     4501 cggctgcgtt cgttgcgttg gcattagcag gttgcggctc aatcaataat gtaaccgttt
     4561 ccgaccagaa acttcaggaa cgtgccgcgt ttgccttggg cgtcagtcca aatgccgtaa
     4621 aaatcagcaa ccgcagcaat gaaggcatac gcatcaactt taccgcaact gtgggtaagc
     4681 gcgtgagcca atgctatgtt accagtgtaa tcagcacaat cggcgttacc acttccgatg
     4741 caatttgttt gggaggcgga acgcacaaag gcaaaagtca atgcaatgct ttgcttaaag
     4801 cggcaggccg ttgctaatcc tttattcgga aaagg
//
LOCUS       DAWKST010000149         4777 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.149, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000149 DAWKST010000000
VERSION     DAWKST010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.149"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            297..995
                     /locus_tag="WJL87_001720"
     CDS             297..995
                     /locus_tag="WJL87_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ7800128.1"
                     /translation="MKSKHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLTLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            complement(1052..2056)
                     /locus_tag="WJL87_001721"
     CDS             complement(1052..2056)
                     /locus_tag="WJL87_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ7800129.1"
                     /translation="MQRQTELKNWLQTIYPERDFDLSFAAADADFRRYFRATFSDGSS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGEAAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            2126..4534
                     /locus_tag="WJL87_001722"
     CDS             2126..4534
                     /locus_tag="WJL87_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ7800130.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVRGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPSVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GATFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEMLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            4531..>4777
                     /locus_tag="WJL87_001723"
     CDS             4531..>4777
                     /locus_tag="WJL87_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800131.1"
                     /translation="MTVGNPAGAPFPHKTAFHRQPQTRHQRQDSNDKERMMKIKALMI
                     AAALLAAADVHAAPQKAKTASAKAAKAAKAAKAAKAAK"
BASE COUNT         1159 a         1457 c         1255 g          906 t
ORIGIN      
        1 ttgaaagggg tgtaaccctt ccgaaccagg gcaacgcata gggcggtgct ttatgtgccg
       61 tcctgtgtgt tggaacatca ttagttcccc aacagccgta tccgtctggg gcgacggcgc
      121 gtattgtacg acagaaaccg cttcagacgg catcgggttt gatgccgtcc gaagcggttt
      181 ccccaaacga aaacgtcctt ttccggaggc aaactgtatc aacacgcgga aatgcaataa
      241 aatagccgga ttccgattga tttccaacat ctgtttccaa catcacggag agccgtatga
      301 aatccaaaca cctcgccctc ggcgttgccg ccctgttcgc acttgccgcg tgcgacagca
      361 aagtccaaac cagcgtcccc gccgacagcg cgcctgccgc ttcggcagcc gccgccccgg
      421 cagggctggt cgaagggcaa aactatactg tccttgccaa cccgattccc caacagcagg
      481 caggcaaagt cgaagtcctt gagtttttcg gctatttctg tccgcactgc gcccacctcg
      541 aacctgtttt aagcaaacac gccaagtctt ttaaagacga tatgtacctg cgtaccgaac
      601 acgtcgtctg gcagaaagaa atgctgacgc tcgcacgcct cgccgccgcc gtcgatatgg
      661 ctgccgccga cagcaaagat gtggcgaaca gccatatttt cgatgcgatg gtcaaccaaa
      721 aaatcaagct gcaagagccg gaagtcctca aaaaatggct gggcgaacaa accgcctttg
      781 acggcaaaaa agtccttgcc gcttacgaat ctcccgaaag ccaggcgcgc gccgacaaaa
      841 tgcaggagct gaccgaaacc tttcaaatcg acggtacgcc cacggttatc gtcggcggca
      901 aatataaagt cgaatttgcc gactgggagt ccggtatgaa caccatcgac cttttggcgg
      961 acaaagtacg cgaagaacaa aaagccgcgc agtaagcccg tttgaaaaat gccgtctgaa
     1021 acttggtttt cagacggcat tttgattggg tttaaaacgt aaagcccgtt tccagttctt
     1081 catcgccgac cagttcgacc aagagcgcgt agagcggggc gagttcggca taacggcgcg
     1141 atacgcggcg caggtaattg aggaaacgcg ggatttcggg acggtatttg tctttgccgt
     1201 cacggtagta caggcgtgcg aagatgcctg caaccttcaa gtgccgctgc acgcccatcc
     1261 attcgaacca gcggtaaaac tcgtcaaacg cttcggggac gggcaagccg gcagcccgcg
     1321 ccttttccca gtagcggata accaagtcca agacaaattc ttcttcccat tcgataaagg
     1381 catcgcgcaa cagcgacacc aaatcgtagg aaatcgggcc gtaaagcgcg tcttggaagt
     1441 ctaaaacgcc cggtctgccg cgcgtcagca tcaggttgcg gacgataaag tcgcggtgca
     1501 catagacttt gggctgcgcc aacaggggcg gcagcagcgt atcgacggtt tgctgccaaa
     1561 gttggcgttg tttgaatgtt aattcgcgcc ccaattcttt tgcgacaaac cattccggga
     1621 acaggttgat ttcgcgcagc atcgtttcac ggtcatattc gggcaaaacc ccttcacggc
     1681 ttgccttctg caattcgacc agctcgccga ttgcctccaa aagcagggct ttgtgcgccg
     1741 cttcgccctg ctcctgaagc atcgcggtca aaaacgtcgt attgcccaag tcgttcaata
     1801 ccacaaaccc caaatccgtg tccgcgtgca atacctgcgg tacattgacc atgtcaaaca
     1861 gtttctgcac tttcaaataa ggtgcgacac tcatcttgtc gggcggtgca tccatgcaga
     1921 cgacactgct gccgtctgaa aacgttgcac ggaaatagcg gcggaaatca gcatccgccg
     1981 ccgcgaagga cagatcgaag tcccgttcgg gatagatggt ctgaagccaa tttttcagtt
     2041 cggtttgccg ttgcataaca gtactaaagc atttcaggtt acaataaacg ctattctaac
     2101 tggcaaaccg acttgagggg cgattttggc tcgtttattt tcactcaaac cactggtgct
     2161 ggcattgggc ttctgtttcg gcacgcattg cgccgccgcc gatgccgttg cggcggagga
     2221 aacggacaat ccgaccgccg gaggaagcgt tcggagcgtg tccgaaccca tacagcctac
     2281 cagcctgagc ctcggttcga cctgcctgtt ttgcagtaac gaaagcggca gccccgagag
     2341 aaccgaagcc gccgtccgag gcagcggcga agcatccatc cccgaagact atacgcgcat
     2401 tgttgccgac aaggtggaag gacagtcgca ggtgcaggtg cgtgccgaag gcaacgtcgt
     2461 cgtcgaacgc aaccggacga ccctcaatac cgattgggcg gattacgacc agtcgggcga
     2521 caccgttacc gcaggcgacc ggttcgccct ccaacaggac ggtacgctga ttcggggcga
     2581 aaccctgacc tacaatctcg agcagcagac cggggaagcg cacaacgtcc gtatggaaac
     2641 cgaacacggc ggacggcggc tgcaaagcgt cagccgcacc gccgaaatgt tgggcgaagg
     2701 gcattacaaa ctgacggaaa cccaattcaa cacctgttcc gccggcgatg ccggctggta
     2761 tgtcaaggcc gcttccgtcg aagccgatcg ggaaaaaggc ataggcgttg ccaaacacgc
     2821 cgccttcgtg ttcggcggcg ttcccatttt ctacacccct tgggcggact tcccgcttga
     2881 cggcaaccgc aaaagcggcc tgcttgttcc ctcactgtcc gccggttcgg acggcgtttc
     2941 cctttccgtt ccctattatt tcaaccttgc ccccaatctc gatgccacgt tcgcgcccag
     3001 cgtgatcggc gaacgcggcg cggtctttga cgggcaggta cgctacctgc ggccggatta
     3061 tgccggccag tccgacctga cctggctgcc gcacgacaag aaaagcggca ggaataaccg
     3121 ctatcaggcg aaatggcagc atcggcacga catttccgac acgcttcagg cgggtgtcga
     3181 tttcaaccaa gtctccgaca gcggctacta ccgcgacttt tacggcaaca aagaaatcgc
     3241 cggcaacgtc aacctcaacc gccgtgtatg gctggattat ggcggcaggg cggcgggcgg
     3301 cagcctgaat gccggccttt cggttctgaa ataccagacg ctggcaaacc aaagcggcta
     3361 taaagacaaa ccgtatgccc tgatgccgcg cctttccgcc gattggcgca aaaacaccgg
     3421 cagggcgcaa atcggcgtgt ccgcacaatt tacccgcttc agccacgaca gccgccaaga
     3481 cggcagccgc ctcgtcgtct atcccgacat caaatgggat ttcagcaaca gctggggtta
     3541 cgtccgtccc aaactcggac tgcacgccac ctattacagc ctcaaccgct tcggcagcca
     3601 agaagcccga cgcgtcagcc gcactctgcc cattgtcaac atcgacagcg gcgcaacttt
     3661 tgagcggaat acgcggatgt tcggcggcgg agtcctgcaa accctcgagc cgcgcctgtt
     3721 ctacaactat attcctgcca aatcccaaaa cgacctgccc aatttcgatt cgtcggaaag
     3781 cagcttcggc tacgggcagc tctttcgcga aaacctctat tacggcaacg acaggattaa
     3841 caccgcaaac agcctttccg ccgccgtgca aagccgtatt ttggacggcg cgacggggga
     3901 agagcgtttc cgcgccggca tcggtcagaa attctatttc aaggatgatg cggtgatgct
     3961 tgacggcagc gtcggcaaaa aaccgcgcaa ccgttccgac tgggtggcat ttgcctccgg
     4021 cagcatcggc agccgcttca tcctcgacag cagcatccac tacaaccaaa acgacaaacg
     4081 cgccgagaac tacgccgtcg gtgcaagcta ccgtcccgca cagggcaaag tgctgaacgc
     4141 ccgctacaaa tacgggcgca acgaaaaaat ctacctgaag tccgacggtt cctattttta
     4201 cgacaaactc agccagctcg acctgtccgc acaatggccg ctgacgcgca acctgtcggc
     4261 cgtcgtccgt tacaactacg gttttgaagc caaaaaaccg atagaaatgc tggcgggtgc
     4321 ggaatacaaa agcagttgcg gctgctgggg cgcgggcgtg tacgcccaac gctacgttac
     4381 cggcgaaaac acctacaaaa acgctgtctt tttctcactt cagttgaaag acctcagcag
     4441 tgtcggcaga aaccccgcag acaggatgga tgtcgccgtt cccggctata tccccgccca
     4501 ctctctttcc gccggacgca acaaacggcc ctgaccgtcg gaaacccggc aggagcaccg
     4561 ttcccgcaca agacggcatt ccaccgacaa ccccaaaccc gccatcaaag gcaggattca
     4621 aacgataagg aaagaatgat gaaaatcaaa gccctgatga ttgccgccgc attgctggca
     4681 gcagccgatg tccacgccgc accgcaaaag gcaaaaaccg catccgccaa agctgccaaa
     4741 gctgccaaag ctgccaaagc tgccaaagct gccaaag
//
LOCUS       DAWKST010000150         4776 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.150, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000150 DAWKST010000000
VERSION     DAWKST010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.150"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            214..993
                     /gene="pgeF"
                     /locus_tag="WJL87_001724"
     CDS             214..993
                     /gene="pgeF"
                     /locus_tag="WJL87_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ7800132.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDNPEAVRRNREIVQQQVGLPVAYLNQIHSTIVVNAAEALDGTPDADAS
                     VDDTGKVACAVMTADCLPVLFCDRAGTTVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            1045..1524
                     /locus_tag="WJL87_001725"
     CDS             1045..1524
                     /locus_tag="WJL87_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800133.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            1526..2524
                     /locus_tag="WJL87_001726"
     CDS             1526..2524
                     /locus_tag="WJL87_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ7800134.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPSKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIG
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHTVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVLRVCRLLDGLREEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEEGDAWTVFKRLVVSLAE"
     gene            2645..3064
                     /locus_tag="WJL87_001727"
     CDS             2645..3064
                     /locus_tag="WJL87_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800135.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAIIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            3140..3655
                     /locus_tag="WJL87_001728"
     CDS             3140..3655
                     /locus_tag="WJL87_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ7800136.1"
                     /translation="MMILKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLYLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(3739..4623)
                     /locus_tag="WJL87_001729"
     CDS             complement(3739..4623)
                     /locus_tag="WJL87_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEZ7800137.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDLRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDAENGSQSLELNRDLVGQLAEINKDLR
                     KHHDLAKLGVADILRFPGVLASQRENTEELAKSITELTEKALKDFTAARKREGKKLGE
                     HLLQRLEAMEEIIDALSELFPTLLETHKEKIRARLVEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIATECTQAS
                     VELKVLIEQMREQVQNIE"
BASE COUNT         1065 a         1262 c         1337 g         1112 t
ORIGIN      
        1 gccgtactgg tttttgttaa tccactatat aaagctcgga gaaggtatat aagggcatca
       61 ttcccgcacc tgcaaaaatc cgaacccgaa cgtttgaaaa caatcccgaa tctccgaatt
      121 cccgcctgtg cgggaatgac gaaaaaacaa acatccattt gccccgaagg cagttaatca
      181 accctttccg ccacacacct attccaatat ccaatgaaaa ccatcacaga aaccctaaat
      241 cttgccccga aaggcaaaaa cttcctgacc gccgattggc ccgcgcccgc caatgtgaaa
      301 accctgatta ccacgcgcaa cggcggcgtg agccaaggtg cgtatcagag tttgaacctc
      361 ggtacgcacg tcggcgacaa tcccgaagcc gtgcgccgca accgcgaaat cgtgcaacag
      421 caggtcggat tgcccgttgc ctacctcaat caaatccaca gcaccattgt cgtcaatgct
      481 gccgaagcat tggacggcac gcccgatgcg gacgcttccg tggacgacac gggcaaggtt
      541 gcctgtgccg tgatgaccgc agactgcctg cctgttttgt tttgcgacag ggcgggtacg
      601 acggttgccg ccgcacacgc gggctggcgc ggtttggcgg gcggcgtatt gcaaaacacc
      661 attgcagcga tgaaggttcc gcctgtcgaa atgatggcgt atctcggccc cgccatcagt
      721 gcggatgcgt ttgaagtcgg acaggacgtg tttgatgcgt tctgcacgcc catgcccgaa
      781 gccgccgccg catttgaagg cataggcggc ggcaaattcc ttgccgacct ttacgcgctc
      841 gcccgcctga ttctgaagcg cgaaggcgtg ggcggcgtgt acggcggcac gcattgtacg
      901 gttttggaac gggatacttt cttttcctac cgccgcgacg gagcgacagg gcgtatggcg
      961 agcctgattt ggctggacgg caatgccgtc tgaacacgcc gctgatataa tctaccgact
     1021 ttgtgctttt gagaaaggca agccatgaac aaactgtttc ttactgccgc agtgctgatg
     1081 ctgggcgcgt gcggtttcca cctgaaaggt gcagacggca tttctccgcc gctgacctac
     1141 cggagctggc acatcgaagg cggacaggca ttgcagtttc ctttggaaac cgcgctgtat
     1201 caggcttcgg gcagggtgga cgatgctgcc ggcgcgcaga tgaccctgcg tatagacagc
     1261 gtttcccaaa acaaggaaac ctacaccgtt acccgtgcgg cagtcatcaa cgaatatctt
     1321 ttgatattga cggttgaagc gcaggtattg aaacgcggcg agccggtcgg caaaccgatg
     1381 accgtgtccg tccgccgcgt ccttgcttat gccgacaacg agatcttggg caaacaggaa
     1441 gaggaagcgg cattgtgggc ggaaatgcgg caggatgccg ccgaacagat tgtccgccgc
     1501 ctgacctttc tgaaggcgga atgacgtggc ggcacatatc ggacgcattg atacggacgc
     1561 gcctttgaaa cccctgtacg tcatccacgg cgaggaagaa ctgttgcgta tcgaggcatt
     1621 ggacgcattg agggcggcgg cgaagaaaca aggttacctt aatcgggaag tttatacggc
     1681 agacaatgct ttcgattgga acgagctgct gcaaaccgca ggcagtgcgg gtctgtttgc
     1741 cgatttgaag ctgttggaac tgcatatccc taacggcaag cccagcaaaa ccggcggcga
     1801 ggcgttgcag gattttgccg cccgcctgcc ggaagatacg gtaacgctgg ttttactgcc
     1861 caaactggag aaaacccagc tccagtccaa atggtttgcc gcattggcgg caaaggggga
     1921 agtgtgggaa gccaaaccgg tcggcgcggc ggctttgccc caatggatac gcggacggct
     1981 ggacaaaatc ggtttgggta tcggggcaga cgcattggca ctgtttgccg agcgcgtgga
     2041 aggcaatctg ttggcggcac gtcaggaaat cgacaagctc gcgctgctgt atccgaaagg
     2101 gcataccgtc aatatcgatg aggcgcaaac cgccgttgcc aacgtcgccc gcttcgacgc
     2161 gttccaactg gcaggcgcgt ggatgaaggg cgatgtcctg cgcgtatgca ggcttttgga
     2221 cggattgcgg gaagagggcg aagaaccggt gctgttgctg tgggcggttg ccgaagacgt
     2281 gcggacgctg atccggcttg ctgccgccct gaagcagggg cagagcatcc aatccgtccg
     2341 caacagcctt aggctttggg gcgacaagca gacgctcgca ccgcttgcag tcaagcggat
     2401 ttccgtcgtc cgcctgcttg acgcgctcaa aacctgtgcc caaatcgacc gaatcatcaa
     2461 aggcgcggaa gagggcgacg catggacggt attcaaacgg cttgtcgtgt cgctggcgga
     2521 ataaagcggt aatccccaaa atccgaaaat actgtaaaat accgttaatc ctgaaaagta
     2581 ttcaccaatc cgtccgaaaa catttcagac ggcacgacca cctcaataaa ggaacattaa
     2641 ccctatggac aataagacca aactgcgctt gggcggcctg attttactga ccaccgccgt
     2701 tttaagcctc attatcgtat tgattgtcga ttcctggccg cttgccatcc tgcttgccgc
     2761 catcatcgtc gccgccgctg cgggcggttt tgtttggaca tcccgccgac agcaacgcca
     2821 gtttatcgaa cgcctgaaaa aattcgacat cgatcccgaa aaaggcagaa tcaacgaggc
     2881 aaacctgcgc cgtatgtacc acagcggcgg acaacaccag aaagatgcga ttaccctgat
     2941 ctgcctgtcg caaaaatgtt cggtggacga ggcgcacgct atgttcaaaa aacgcccgac
     3001 acgtcaggaa atcaatcaaa tggcggcaaa acagtcgcgc ggtcagaaac gtccgcaccg
     3061 ttaaccgccg caaggcatct ttgcataaat gccgtctgaa gcctgttggc gtttcagacg
     3121 gcatattctg attgaaaaga tgatgatact gaaaaccgcc ccgctcaaac gccgctttgc
     3181 cgccatgctg tatgagatgc tgctggtcgg tgcggcaacc tgtttggcag cattgtttgc
     3241 cggtattgcc gccatttttc tgaatcccgt ttctatcgcg gtttctgcat tggtaacgag
     3301 tatcctgata atgggatcat ggtggcttta ttttcgcgcc aactggcacg gtcaggggca
     3361 gaccttggcg atgaggacat ggaaaatcgg cttgtgcgac cttaacggca tacagccgtc
     3421 tttgtacctg ttgcgcctgc gctttatttg ggcgtgcata ttcatcgtct tcattcctat
     3481 gcttgcttat gccggattgc gccacttcct cggcattccg cccaagggcg cggccggcgc
     3541 ggcattgatt tggctgattt taccgtgggg gttcgcactg ctgaatcccg accggcagtt
     3601 tctgtatgat tttcttgcag gaacaagatt ggtggcggtc aaaggaaagc cttaagcctt
     3661 tataccgcaa aggtttcaaa ctgaaaaaat gccgtctgaa agggctttca gacggcattt
     3721 gcttatcggg gaaaccgatt attcgatatt ctgcacttgt tcgcgcatct gttcgatcag
     3781 gactttcagc tcgaccgagg cttgggtgca ttcggtagcg atggatttgc tgcccaaagt
     3841 gttggcttcg cggtttaatt cctgcatcag gaagtccagc cgtttgccgc tgctgccttt
     3901 gtgttcggta acgatacggc gcacttcggc aatgtgggtg cgtaggcggc tgaactcttc
     3961 gtcgatgtcg gatttttgga taaagagggc aaattcctgt tgcaggcggt cgttgtcgat
     4021 gctgccgacc gcttcgacga ggcgggcgcg gattttttct ttatgtgttt ccaacagggt
     4081 aggaaagagt tcgcttaatg catctatgat ttcttccata gcctcaaggc gttgcagcag
     4141 gtgctcgcct aatttcttac cttcccgctt gcgtgcggca gtaaagtctt ttaacgcttt
     4201 ttcggtcagt tcggtaatgc tttttgccaa ttcttccgta ttttcccttt ggcttgccaa
     4261 tacgccgggg aaacgcagga tgtcggcaac gcccagtttt gccaaatcgt gatgcttgcg
     4321 gaggtctttg ttgatttcgg caagctgtcc gaccaagtcg cgattcagtt ccaaggactg
     4381 actgccgttt tccgcatctt gaatttggat tttgcattcg actttgccgc gtgcgatatg
     4441 ggatgaaatt ttctcgcgga taccgctttc caaatagcgc aaatcgtcgg gcatcctgat
     4501 ttgaatatct agaaaacggt ggttgaccgc acgcaggtcg agattgatgc gtttgctgcc
     4561 gcactctgcc gccgcgttgg caaatccggt catgctgtgg atgtggatat ttccgctgct
     4621 catgtcgttc tccgaagccc gttaaaatgg aatcaatata tcacatctgt atggcggcaa
     4681 gcgttttcgg gtgtgaaaaa ttgaagattt tgcagcggca gattggaatc acgcgctttt
     4741 gttgctgcaa ggaagggaaa tgtatagtgg attaac
//
LOCUS       DAWKST010000151         4555 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.151, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000151 DAWKST010000000
VERSION     DAWKST010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.151"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            291..1412
                     /gene="dnaJ"
                     /locus_tag="WJL87_001730"
     CDS             291..1412
                     /gene="dnaJ"
                     /locus_tag="WJL87_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ7800138.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1608..3626
                     /locus_tag="WJL87_001731"
     CDS             1608..3626
                     /locus_tag="WJL87_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ7800139.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPAAERAEQDLSPKAMIFWVLAMMFVLGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIAADLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMIIFARRVLKAGR"
     gene            complement(3670..4227)
                     /gene="rfbC"
                     /locus_tag="WJL87_001732"
     CDS             complement(3670..4227)
                     /gene="rfbC"
                     /locus_tag="WJL87_001732"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ7800140.1"
                     /translation="MNIIDTAIPDVKLLEPQVFGDARGFFMETFRDEWFKTQVCERTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMRKDSPTFGKWVGEILS
                     AENKRQLWVPEGFAHGFYVLSDEAEFVYKCTDYYNPEAEHSLIWNDPTVGIDWPLQGE
                     PNLSPKDLAGKTWAQAEKLRLTLSR"
     gene            complement(4281..>4555)
                     /locus_tag="WJL87_001733"
     CDS             complement(4281..>4555)
                     /locus_tag="WJL87_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HEZ7800141.1"
                     /translation="DLNRMYLEDGSLSVQILGRGFAWLDTGTHESLHEAASFVQTVQN
                     IQNLHIACLEEIAWRNGWLSDEKLEELARPMAKNQYGQYLLRLLKK"
BASE COUNT          953 a         1037 c         1349 g         1216 t
ORIGIN      
        1 gctgcccgcg gataaaaaat aaaataaggg gaagtctgca gccgcatcaa atgccgtctg
       61 aacatgcgtt cgggcggcgt ttttataaca aaaacacttc atggcggttg gttttatgcc
      121 tgtctaagtt tttgtgtcgt gcatacctga agatttcaga cggcatcggt ttatgctgtc
      181 tgaaaagtgt attccgtttc agtttgtaag ctatggcagt ctgtttgtct tgtgttttgc
      241 gcaattgccc ttattttgag ccgtgatttt attttgaatt agatgaaaaa atgagtaatc
      301 aagattttta tgcgacgctg ggtgtggcaa gaacagctac cgatgatgag attaaaaaag
      361 cctaccggaa attggcaatg aaataccatc ccgaccgcaa tcctgacaat aaagaggcgg
      421 aagagaagtt taaagaagta caaaaggcgt atgaaacttt gtccgacaag gaaaagcgcg
      481 ctatgtacga ccagtatggt catgcggcgt ttgaaggcgg cggacagggg ggcttcggag
      541 ggtttggcgg atttggcggt gcgcagggtt ttgactttgg ggatattttc agccaaatgt
      601 ttggaggcgg ttcggggcgc gcccagcctg attatcaggg tgaggacgtt caagtcggta
      661 tcgaaatcac gcttgaagaa gccgcaaaag gtgtgaagaa acgcatcaat attccgactt
      721 atgaagcgtg tgatgtctgt aacggcagtg gcgcgaaacc ggggacatcc ccggaaacct
      781 gcccgacttg caaaggttcg ggtacggtgc acatccagca ggcgattttc cgtatgcagc
      841 agacttgtcc gacctgccac ggtgcgggca aacacattaa agaaccttgc gtcaaatgcc
      901 gtggcgcggg gcggaataag gcggtcaaga cggtggaagt caatattccc gccggtatcg
      961 atgacgggca gcgtatccgt ttgagcggcg aaggcgggcc gggtatgcac ggtgcgcctg
     1021 ccggcgactt gtatgtaacc gtccgcattc gggcgcataa gattttccaa cgcgacggtc
     1081 tggacttgca ttgcgaactg ccgatcagtt ttgccacggc tgctttgggc ggggagttgg
     1141 aagtgccgac cttggacgga aaggtcaagc tcaccgtccc caaagaaacc caaaccggca
     1201 ggaggatgcg cgtgaagggt aagggtgtca aatctttacg cagcagcgcg accggcgatt
     1261 tgtactgcca tattgttgtc gaaacgcctg tcaatttgac cgaccgtcaa aaagagcttt
     1321 tggaagaatt tgagcggatt tctaccggct tggaaaacca aacaccgcgc aagaaatcgt
     1381 ttttagacaa gctgcgcgat ttgtttgatt gattttaagg ttcggaaaca agcagccgta
     1441 tcggggaatc tccctgatac ggctgttttt gtttgtttaa aaatagtttt tattttcaat
     1501 ggggtatggg gcagggtggg ataactgttt ttaactgttc tttttaaaac ttgacatcat
     1561 ggcgcgatgc caacaatatg tgaacgtctg ttgtcaaagg aagaataatg aataaatctt
     1621 tatccgggtc ggtagaagaa taccgcgaac tgacgctccg aggcatgata ctcggtgcat
     1681 tgatcactgt aatttttact gcgtccaacg tttacctcgg tttgaaagtc gggctgacct
     1741 ttgcctcgtc gattccggcg gcggtgattt cgatggcggt tttaaagttt ttcaaaggca
     1801 gcaatatttt ggaaaacaac atggtgcaga cccaagcttc ggcggcgggt acgctttcga
     1861 ccatcatctt cgtcctgccc ggtttgctga tggcgggcta ctggagcggt ttcccgttct
     1921 ggcagacgac gcttttatgt attgccggcg ggattttggg ggtgattttc accattccgc
     1981 tgcgttacgc gatggtggtg aaaagcgatt tgccttatcc ggaaggtgtg gcggctgctg
     2041 aaattttgaa agtgggcggt catgaagaag gggataaccg tcagggcggc agcggcatca
     2101 aagagctggc ggccggcggt gcgttggcgg gattgatgag cttttgcgcc ggaggtctgc
     2161 gcgtgattgc cgacagcgcg agttattggt ttaaaagcgg tacggcgatt ttccagctgc
     2221 cgatgggctt ttcactggca ttgttgggcg cgggctattt ggtcggactg acgggcggta
     2281 tcgccatcct gttgggcatt tcgattgctt ggggcattgc cgtgccgtat ttctcctcac
     2341 acattccgca accttccgat atggaaatgg cggcgtttgc gatgaagctg tggaaggaga
     2401 aagtgcgttt tatcggtgcg gggactattg gcattgcggc ggtttggacg ctgttgatgc
     2461 tgctcaagcc gatggtggaa ggcatgaaga tgtcgttcaa gagttttggc ggcggtgcgc
     2521 ccgctgcgga acgcgccgaa caggatttgt cgcctaaggc tatgattttt tgggtgctgg
     2581 cgatgatgtt tgttttaggc gtgtcgtttt accactttat cggcgattcg cacattacgg
     2641 gcggcatggc ttggcttttg gtggtcgttt gcacgctttt ggcttccgtc atcggctttt
     2701 tggtcgccgc cgcctgcggt tatatggcag gtttggtcgg ctcgtcttcc agcccgattt
     2761 ccggcgtggg catcgtgtcc gtcgtcgtta tttcactggt tttgctgctg gtaggcgaat
     2821 ccggaggttt gttggcggat gaggctaacc gcaaattttt gctggcactg actttgtttt
     2881 gcggctcggc agtaatctgc gtggcttcga tttccaatga caacctgcaa gacttgaaaa
     2941 ccggctacct gctcaaagcc acgccttggc ggcagcaagt cgccctgatt atcggctgta
     3001 tcgttggtgc gctggttatt tcgcccgtgt tggaactgct ttacgaagcc tacggcttta
     3061 ccggcgcaat gccgcgcgaa ggcatggatg cggcgcaggc tttggcagcc cctcaagcga
     3121 ctttgatgac gaccatcgcg tcgggcattt tcgcccacaa ccttgaatgg gtctatatct
     3181 ttaccggcat cgcgattggc gcggtgttga ttgccgccga tttggtgttg aaaaaatcat
     3241 caggcggcaa acttgccctg cccgtccttg cggtcggtat gggtatttat ctgccgccgt
     3301 ccgtcaatat gcccatcgtg gcaggcgcgg tgttggcggc ggtgttgaaa cacatcatcg
     3361 gtaaaaaagc ggaaaaccgt gaaggccgtc tgaaaaacgc cgagcgcatc ggaaccttgt
     3421 tctccgccgg tctgattgtc ggtgaaagtc tgattggtgt gattatggcg tttattattg
     3481 ccttctccgt gaccaacggc ggctcggacg cgccgctcgc gttgaatctg caaaactggg
     3541 atgccgccgc ttcttggctg ggcttggcgt tcttcgttac cggaatgatt atttttgcac
     3601 ggcgcgtact gaaggcgggt aggtagcctg tcggagaaaa tgccgtctga aacgttcaga
     3661 cggcattttt tatcgggaaa gtgtaaggcg gagcttttcg gcttgcgccc acgttttgcc
     3721 ggcaaggtct ttgggcgaca ggttcggctc gccttgtagc ggccagtcga tgccgacggt
     3781 cggatcattc caaattagtg agtgttcggc ttcagggttg taatagtctg tgcatttata
     3841 gacgaactcg gcttcatcgc tcagtacata gaagccgtgt gcgaaacctt cgggtaccca
     3901 cagttggcgt ttgttttctg cggacagaat ctcgcctacc catttgccga aagtggggga
     3961 gtctttacgc atatcgacgg ccacgtcgaa tacttcgccg acaactacgc gtacgagttt
     4021 gccttgtgtg ttttcggttt ggtagtgcag gccgcgcaat acgcctttgc cggatttgga
     4081 gtggttttcc tgcacgaagg tgcgttcgca gacttgggtt ttaaaccact cgtcgcggaa
     4141 ggtttccata aaaaagccgc gcgcgtcgcc gaagacttgg ggctcaagca gttttacgtc
     4201 aggaatggcg gtatcaatga tgttcatctt tttatctttc atctaaaggc cgtctgaaaa
     4261 gtttcagacg gcctcaaaca ttattttttc aacaggcgca gcaaatattg gccgtattgg
     4321 tttttcgcca tcgggcgcgc caattcttcc agtttttcat cggaaagcca accgttgcgc
     4381 caagcgattt cttccaggca ggcgatgtgc aggttttgga tgttttgcac ggtttggacg
     4441 aacgaagccg cctcgtgcag gctttcgtgc gtaccggtgt ccagccacgc gaaaccgcgt
     4501 cccaatattt gaacggagag cgagccgtct tccaaataca tccggttgag gtcgg
//
LOCUS       DAWKST010000152         4533 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.152, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000152 DAWKST010000000
VERSION     DAWKST010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.152"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(238..345)
                     /locus_tag="WJL87_001734"
                     /pseudo
     CDS             complement(238..345)
                     /locus_tag="WJL87_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(391..1119)
                     /gene="pdxJ"
                     /locus_tag="WJL87_001735"
     CDS             complement(391..1119)
                     /gene="pdxJ"
                     /locus_tag="WJL87_001735"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ7800142.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(1143..1934)
                     /gene="recO"
                     /locus_tag="WJL87_001736"
     CDS             complement(1143..1934)
                     /gene="recO"
                     /locus_tag="WJL87_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ7800143.1"
                     /translation="MSEHRVNHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSASWYGSQELKTLHRAEWIGGWPQPQGRALFSGLYVNELMLK
                     LTVREDPLPELYDALAEVMEAVCCKAAYIDDLRRFEWRLLNLLGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCEDADALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            complement(1968..3095)
                     /gene="pheA"
                     /locus_tag="WJL87_001737"
     CDS             complement(1968..3095)
                     /gene="pheA"
                     /locus_tag="WJL87_001737"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ7800144.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(3134..4366)
                     /locus_tag="WJL87_001738"
     CDS             complement(3134..4366)
                     /locus_tag="WJL87_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEZ7800145.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLTVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVASMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQHFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRITAWRL
                     KIGVHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENGQSEYL"
BASE COUNT         1014 a         1306 c         1242 g          971 t
ORIGIN      
        1 acataatcat cgcctcaata tttaatatcc caaaatatag atttaaaacc atcgcctttg
       61 tggtttgtca gcattcctaa ttgagataga aagtcaaata atttcttgtt tctccggctg
      121 ccatcaagat ggtttagatt aagacctcga acaatacaat acccaatact ctgtactgta
      181 ttgtgaattt cttctatcaa actattattg tttgcagata aaatatactt ttgattcata
      241 tagtgaatta aatttaaacc agtacggcgt tggcttgcct tgccgtacta tttgtactgt
      301 ctgcggcttc gccgccttgt cctgatttaa atttaatcca ctataacata tccctgtcgt
      361 gaaatgctgt ctgaaagggt ttgcctgccc ttacggcagc agccttgccc tgaacatcgc
      421 ctccttcatt tggcgcacgg cttcgggcag tccgaggaag agggcttggg caatcagcga
      481 atgcccgatg ttcagttcgc ggatggcgag gatttgggcg atgggggtaa cgttgtgtat
      541 ggtcagtccg tgtccggcgt tgacgaccaa gcccaaatcg ccggcaaaat gcgcgccgtt
      601 ttgcagacgc tcgaattgct tgagttgttc ggcgtggctg cgcgcgtcgg catacgcgcc
      661 tgtgtgcagc tcgacaacgg gcgcgccgac atcataggcg gcttggattt gcctgtcgtc
      721 ggcatcgata aagagagaga ctcggatgcc tgcgtcggtc aggattttgg tgaacccggc
      781 gattttttcc tgttgcgcca atacgtccaa gccgccttcg gtcgtgattt cctgacgttt
      841 ttcaggcacg atgcacacgt cttccggcat cactttaagc gcgttttcga gcatttcttc
      901 cgtcaacgcc atttcaaggt tcaggcgcgt gcggatggcg tttttgacgg caaacacatc
      961 cgcgtctttg atgtggcggc ggtcttcgcg caggtgcatg gtaatcaaat ccgcaccgtg
     1021 cgtttcggca accagtgccg cctccacggg gctgggataa gtcgtgccgc gcgcattgcg
     1081 gacggtagcg atgtggtcga tgttgacacc taaaagcata atctttcctt ttatttctgc
     1141 cttcagacgg catttgaagc cgtgccgtcc gaagtcggga cggtttcccg ggcggtttct
     1201 ttgcggtcaa actgccgtat ctgttccaac acctgccgcg atttcagccc ctcgggcaac
     1261 aggtggcgga taaaaagccg tgtgattttc aatgcctgtt gcaggctttc ggcggtgcgg
     1321 aaactgcctt cgcgcaaatc gatcaggctt tgcccttcga caattgcgcc ggcttcgtgc
     1381 gaaagcgcgt ctgcgtcctc gcatacgggc atgaccgcct cttcgggcat cagccggtag
     1441 gttttgtccg ccaaaatatc gccgcccgtg ccgtcggcgt gcaaatcggg ggcaacgccc
     1501 aacaggttca gcagccgcca ctcgaaacgg cgcaagtcgt cgatataagc ggctttgcag
     1561 cacaccgcct ccatcacttc cgccaacgcg tcgtataact cgggcaacgg atcttcgcgg
     1621 acggtcaact tcagcatcaa ctcgttcaca tacaatccgc tgaacaaagc cctaccctgc
     1681 ggctgcggcc acccgcctat ccattcggcg cgatgtaggg tcttcaactc ttgcgaacca
     1741 taccaagacg cactcaccgg cacaaacggc accaacacgc cgcgcaattc gctctgccgt
     1801 ttgcgcgcgc ttcttgccaa aagtgccaca cgcccgtaac ggcggctgaa cgcttcgacc
     1861 cgcaggctgc tttcgcgcca gggcgaggaa gccaacagga agacgggttc gtggttgact
     1921 cggtgttcgg acatcatgcc tgaaatgccg tctgaacgct gccgccgcta caaaacggcg
     1981 gtcgggtacg aaccgatgac tttgacgaac gaagcgcgtt cgcccaagcg ttccaatgcc
     2041 gtctgaatct gcgcgtcccg gcggtgtcct tcgatgtcga tgaagaacag gtattcccac
     2101 aaaacggatt tactcggacg gctctcaaac ttggtcatgg aaatacccga ctcagtcagc
     2161 ggttgcagca gcgaggcaac cgcgcctgcc cggttgggcg cggaaacggc cagcgaagtc
     2221 ttgtcgctgc cgcttgcacc ggtttcgtga tgtcccatca ccaagaagcg cgtggtgttg
     2281 ttcggttcgt cttcgatgca ctcggcaacc atatcgagtc cgtagatttc cgccgccgtg
     2341 cgtccggcga tggcggcaac cgtaccgtcg tccgattcgg caaccagcct tgcggcttcg
     2401 gcattgctgg acacggcaat ccgttcggcg ttgggcaggt gtctgcccaa ccagtcgttg
     2461 cactgcgcca acgcctgcgc gtgggaaaag actttggcaa tgccttcggt gctgccgttg
     2521 tttttacgca aaaggttgtg gtggatgcgc aaaacgactt cgccgcacgc ctgcaacgcg
     2581 gtaacggcaa gcaaatccaa cgtgcgcccg accgagcctt cggtcgagtt ttccacgggc
     2641 gcgaccagat aatccgcctg acgggtttca acctgcttga agcagttgtc tatggtcgga
     2701 cacgccatgg tgtgcgcggc gtgtccgaaa tgcttgatcg ccgcctgctg ggtaaacgtg
     2761 ccctgcggcc ccagataggc gatggtcagc gggcgttcta cggcgaggca ctcgctcatc
     2821 acttcccgaa acaggcgtgc taccgattcg tcgggcagcg ggcctttgtt caaatcctga
     2881 atgcggcgca acacggcgac ttcgcgttcg gggcggtaca ctgcgcccgt gcctttcagc
     2941 tcgccgatgg cgtgggcgtg ttgcgcacgt tcgttgagca ggcgcaggat ttcggcatcg
     3001 atggtgtcga tggcgttgcg gtggggaagg aggagttcgt cgatagtttg ggacataatt
     3061 tcgcctgaac agtaatttat cgtgcattct aacatcaaat gccgtctgaa acggtttcag
     3121 acggcatttt ccgttaaagg tattcgcttt gcccgttttc tttccacgca cgatgcgagc
     3181 agagccacag aagcgcaatg ccgcaagagg tggacgcggt catcgttgcc gccatcacgg
     3241 ttgccgaacc gtcgtgcaag aaggtcgccg ccatacccac ccccgcgccg attaaagatt
     3301 ggaatacacc caatacggcg tttgcgctgc cgccctcttc tttgaaatag gacataaaac
     3361 acgcctgcgt gtttgcaccg accaagccct gcgtaccgac ggaaaacatc acgcacgcga
     3421 ccagcagcca aaacgggggc aacccgaaaa acagcacggc ggcgagttgg gacaggttgg
     3481 cggcaaactg gacgacaatc ccccacagca ggatgctttg cggatgcacg ccgattttga
     3541 gccgccacgc ggtaatgcgg ttgaaaaaca tcatcgtgat gatgttgagt gcaaacgccc
     3601 aagcgtattg atgaggcgta acacggtaga gctgctggta cacgaaggaa gattcggtca
     3661 gaaaggcgaa catcgaaccg aagctgaatg cctgaaaaaa cagataaccc atcgcggcac
     3721 gggttttcaa tacgcgcttg aaccgccccg ccaccagccc gaacacgtcc cgtccgattt
     3781 tgccgccgac ggcaggcttg ggcaggaaat gctgcaccaa accgagcagc accagcgaat
     3841 acgccgccag aaaaacaaaa atcgcctgcc agccacccaa gccctgcaac aatgcgccga
     3901 ccatggatgc gaccagcggc acaaccatca aaatgatgcc gataagggca aacatctggg
     3961 cggctttgcg tccggaataa taatcgcgca ccattgcgcc gacgatgacc acagtcatgc
     4021 ccgcaccgaa tgcctgcacg acgcgcaggt tgaggagctg ttcggcactc gaagcaaata
     4081 cgatggcggc aacggcaagg cagtagacgg tcagtccggt cagcgcgacg ggtttgcgcc
     4141 ctttgatgtc ggacaccgaa ccgccgacca cctgtccgaa cgccgtgccg aacataaaca
     4201 aactcaggct ctgttcgatg cggtggacat ccgcgttcag cgactgcgcc atttcgggaa
     4261 tcgcgggcag gtaggcatcg atggaaaacg gcatcagcgt aaccagcatc gccatcaaaa
     4321 ccgccatcag tttttcgctc atttcaggat aatgggcaga aggcataaat tctcgtccga
     4381 ttcgcaaaca aaccgcccgg cggcggaaaa cacaatacgc ggaaacggaa aattttaaca
     4441 caccggcaca aacaggcggc aacaaaagcc ccggcaaaac cggacgaacc ggaacgatat
     4501 agtggattaa caaaaaccag tacagcgttg cct
//
LOCUS       DAWKST010000153         4530 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.153, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000153 DAWKST010000000
VERSION     DAWKST010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.153"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(58..1851)
                     /locus_tag="WJL87_001739"
     CDS             complement(58..1851)
                     /locus_tag="WJL87_001739"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ7800146.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIVKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFLHESGIHQDGVLKHRET
                     YEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            2097..3206
                     /gene="proB"
                     /locus_tag="WJL87_001740"
     CDS             2097..3206
                     /gene="proB"
                     /locus_tag="WJL87_001740"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ7800147.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIAAESGVPVYICSSLKPDALAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            3219..4481
                     /locus_tag="WJL87_001741"
     CDS             3219..4481
                     /locus_tag="WJL87_001741"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ7800148.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAAAADI
                     LAANRQDLEAAAGKIPESMTDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYVDKDADLEKAIRIVRNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIEAHGTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
BASE COUNT         1038 a         1241 c         1258 g          993 t
ORIGIN      
        1 gccgcaatgc cgtctgaagc ctttttatgc ttcagacggc atcgtctgtt ttcacgctca
       61 aatcgtaccg ctgccttgcg ctttcggttt ggcggcacta aattccagtt tgctcaaagc
      121 ggaaaggtag gctttggcag tggcaaccaa aacgtcggta tccgcgccct gaccgttgac
      181 gacgcggttg ccgcgtgcca gacggacgct ggtttcgcct tggctttccg taccttgcgt
      241 gacggcgttg acggaataaa tctgcaaagt cgcgccgctt tgcgccacgc tttcaatcgc
      301 tttgaaaatt gcatcaaccg gaccggaacc ggttgcggaa gcgcgttttt cttcgccttt
      361 gatgctgaac acgatgtcgg cgcgcggctc ttcgccggtt tcggtgctga ttttttggga
      421 gatgaatttg tagctctcgg cattcatgct gcccatttcg tcggatacca gtgcgtgcag
      481 gtcttcatcg aagatttcgc gttttttgtc ggcgagttct ttgaagcggg caaacgcggc
      541 gttcagtgcc tcttcgcttt ccaactcgat gcccaaatcc gccagcttgg ttttgaaggc
      601 gttgcggccg gacaatttgc ccaagctcaa acggtttgtt gcccagccga ccgattcggc
      661 ggacataatc tcgtaagttt cgcggtgttt cagcaccccg tcctgatgga tgcccgattc
      721 atgcaaaaag gcattggcac cgacaatggc tttgttgggc tgcacgggat agcccgtaat
      781 ggtggacacc agtttggacg aaggcacgat ttgcgtggta tcgatgccgg tttccaagcc
      841 gaacaagtcg tggcgcactt tcaacgccat cacgatttct tcaaccgaag cattgcctgc
      901 acgttcgccc aagccgttga cagtacattc cacctgacgc gcaccgcctt ttaatgcggc
      961 aagcgaattg gcaaccgcca agcccaaatc gttgtggcag tgtgccgacc aaacgacttt
     1021 gccgccgttg ggcgttttga caatcagttc gcggaaaaat tcttcggttt tgtacgggat
     1081 ggaatagccg acggtatcgg gaatattgat ggtggtcgcg cccgcttcaa ttaccgcgcc
     1141 gcagatttcg gcaaggaaat cgatttccga acgcaacgcg tcttcgcagg aaaattccac
     1201 atcgtcggtg tattcacgag cgattttcac cgctttgacc gccgcctcaa tcacctgctt
     1261 cggcttcatc ttcaatttgt actccatatg gatggggctg gtggcgatga aggtgtggat
     1321 gcgttttttc ggcgcgggcg caacggcctc acccgcctga cggatgtccc gctcgatggc
     1381 acgggacaat gaacagaccg ttgatttggt aatggttttc gcaatcgcat tgaccgcctc
     1441 gaaatcgccc gggctggcag cggcaaaacc cgcttcgatg atgtccacac ccaatttttc
     1501 cagctggcgg gcgacgcgga ttttttcctc tttggtcata gcggcgccgg gcgattgttc
     1561 gccgtcgcgc agggtggtgt cgaaaatgat aacgcggttg gcctgtgtca tttctgttct
     1621 ctccagagat tcgttttttt gtaaaaaagc attcaaatcc agcggcctgc cttgcgattc
     1681 ggcaagcgcg gtcagctttt gcagttgcgc caaaggcagc gacccgcgcg tgcgccattt
     1741 atagatggcg gcggtcgtcg cggcattttc gggatcgtgc tgtttcaacg cttcggcaag
     1801 cgcgttcacg ccgccgaaat aagcaaccaa gcggtctata tctaattgca tggtagtcct
     1861 tgtctaaaat aaatcagcct acagcagaaa tatggacagg attatacgca ttttggacaa
     1921 aacggcaata taaatttgaa aaaatctcaa taaaccgcca tagattttat taattttata
     1981 cattttgtcc attttattgc agacgcggcg tgattgttga caattgcggc agaacagggc
     2041 aaattcggcg gcacaaggtg gcaacgagac aggacaaacc aacaaggacg gaaacaatga
     2101 aatacaaaag aatcgtattt aaagtcggca catcttcgat tacccattcg gacggcagtc
     2161 tctcgcgcgg caaaatccaa accatcaccc gccagcttgc cgcattgcat catgcgggac
     2221 acgagctggt cttggtgtct tccggcgcgg ttgcggcagg gttcggtgcg ctgggtttca
     2281 aaaaacgtcc ggtcaaaatc gccgacaaac aggcttccgc cgccgtcggg caggggctgc
     2341 tgatggaaga atatacggca aacctgtctt cagacggcat cgtgtccgca caaatcctgc
     2401 tcagccgcgc cgactttgcc gacaaacgcc gctaccaaaa tgccggcggc gcactttccg
     2461 tgctgctgca acgccgcgcc gtccccatca tcaatgaaaa cgatacggtt tcggttgagg
     2521 aattgaaaat cggcgacaac gacacattga gtgcgcaagt ggcggcgatg atacaggcag
     2581 acctcttggt gctgctgacc gacatagacg gtctttacac gggcaacccg aacagcaatc
     2641 ccgatgccgt acggctggac aaaatcgaac acatcaacca tgaaatcatc gaaatggcgg
     2701 gcggatcggg ttcggcaaac ggcacgggcg gtatgctgac caaaatcaaa gcggcaacca
     2761 tcgccgccga atccggcgta ccggtgtata tctgttcctc gctcaaaccc gatgcacttg
     2821 ccgaagctgc cgaacatcag gcggacggct cgtttttcgt cccccgtgcc aaaggtttgc
     2881 ggacgcagaa gcaatggctg gcgttctatt ccgaaagccg gggcagcgtt tatgtggacg
     2941 aaggtgcgga acacgctttg tccgaacagg ggaaaagcct gctgatgtcg ggcattgccg
     3001 gaatcgaagg gcatttttcc cgtatggaca ccgtaaccgt gtacagcaag gcaaccaaac
     3061 agcccctggg caaagggcgc gtcctgttcg gttctgccgc cgccgaagac ctgctcaaat
     3121 cgcgtaaggc gaaaggcgtg ttcatccatc gggacgactg gatttccatc acgcccgaaa
     3181 tacgcctgct tctgaccgaa ttttagaaaa ggaaacccat gtcaaacaca caaaaacagc
     3241 ttgcccttgc caaagcggca aaaaaatccg tcaacacggc ggatacggaa gaaaaaaacc
     3301 gcgcgctgct tgctatggcg gacagtctgg aagcggcggc ggcagatatt ttggcggcaa
     3361 accgtcaaga cttggaagcc gcggcaggca aaattcccga aagcatgacc gaccgccttt
     3421 tgttggacgg caaacgcatt tgcgcgatgg cggacggcat tagggcggtt gccgcactgc
     3481 ccgaccccgt gggcgaaata ctggaaacct cgactctgcc caacggcttg gaaatcgtca
     3541 aaaagcgtgt ggcgatgggc gttatcggca ttatttacga aagccgcccg aacgttacgt
     3601 ccgatgcggc ggctttggca ctgaaaagcg gcagcgcggt cgtactccgc agcggcaaag
     3661 atgcattcca atccgcacgc gccatcgttg ccgccctgaa aacggggttg gcacaaaccc
     3721 gcatcgaccc cgacgcgctc cagttgattg aagacaccgg gcgtgagggc agttacgaaa
     3781 tgatgagggc gaaagattat ctagacctgc tgattccgcg cggcggggcg gggctgatac
     3841 gggcggtggt tgagaatgcc gtcgtgccgg tcattgaaac ggggacgggc attgtccaca
     3901 tctacgtcga taaagatgcg gacttagaaa aggcaatccg cattgtccgc aacgccaaaa
     3961 ccagccgtcc gtccgtgtgc aactcgatgg aagtgctgct ggtgcatgaa gacattgctg
     4021 ccgacttcct gcccaagctc gaacggctgt tggttcgcga ccgtatagaa gccggactgc
     4081 cgcccgtccg cttccgtttg gatccgcagg cggcgcggca tatcggcggc gaagcggcgg
     4141 gtgcagacga tttcgatacc gagtttttag actacatcct cgctgtgaaa accgtcgcat
     4201 cggtcgaaga ggcggtcggg cacatcgaag cccacggcac acaccattca gacggcatcg
     4261 ttaccgaaaa ccgccacgct gcggactatt tcacgaccca tatcgattct gccgccgtgt
     4321 atgtcaacgc gtccacgcgc tttaccgacg gcggcgaatt cggcttgggt tgcgaaatgg
     4381 gcatctccac gcaaaaactc cacgcccggg gcccgatggg tttgaaagag ctgacgagtt
     4441 acaaatacat cgtacaaggc acgggacagg ttagggaata atccgaattc aaacgtctga
     4501 tatagtggat taactttaaa ccgatacggc
//
LOCUS       DAWKST010000154         4512 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.154, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000154 DAWKST010000000
VERSION     DAWKST010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.154"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(1006..1776)
                     /gene="xth"
                     /locus_tag="WJL87_001742"
     CDS             complement(1006..1776)
                     /gene="xth"
                     /locus_tag="WJL87_001742"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ7800149.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMARHGKLVLLGDFNIAPADADCYDPE
                     KWYEKIHCSTVERQWFQNLLDLGLTDSLRHIHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVTSEMAAVLKDVRVDLDTRALERPSDHAPVTAEFDW"
     gene            complement(1836..2117)
                     /locus_tag="WJL87_001743"
     CDS             complement(1836..2117)
                     /locus_tag="WJL87_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ7800150.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSGHLSRLRVEGLVDFTRYHRIIEYRLISEDAATILRTIRDLENKRAV"
     gene            2174..2452
                     /locus_tag="WJL87_001744"
     CDS             2174..2452
                     /locus_tag="WJL87_001744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800151.1"
                     /translation="MIVIIFINLIINQIFMDDCTGLYVPFPTFFANTRKGGLSDGIKL
                     PPLPCLTLLPNGIIRIVQKMPYNRRTSCPADASVPSGKQNTCLKCCFF"
     gene            complement(2585..3466)
                     /gene="nadC"
                     /locus_tag="WJL87_001745"
     CDS             complement(2585..3466)
                     /gene="nadC"
                     /locus_tag="WJL87_001745"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ7800152.1"
                     /translation="MPSENTLFPLPDTLLRPMVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQVVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGISTATARAVAEVAKYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCDSIAQAVRQAKQAVGPLTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFLA"
     gene            complement(3541..4479)
                     /locus_tag="WJL87_001746"
     CDS             complement(3541..4479)
                     /locus_tag="WJL87_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800153.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVGRLRIWANSADTEEVRQKRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIEEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
BASE COUNT         1040 a         1128 c         1200 g         1144 t
ORIGIN      
        1 atagtggatt aacaaaaatc aggacaaggc gacgaagccg cagacagtac aaatagtacg
       61 gcaacgctgt actggtttaa atttaatcca ctatattttg taatagctgc cattatgcct
      121 actaggatat ataccttcta tataatcctt atttaaataa aaacccttat aaaaatctgt
      181 gttatctgtc gggattgaaa aaaagatttt atctttctca tcaggtttat atttgctatc
      241 aatatcaaaa agagaataag gattacaatg agttcgacat actaggtaaa aatttatatc
      301 ttggatcatg aaatattcga tatttatcag gatcgaaaga tgctaaaaat tcatcaatat
      361 tggtactgga tattttgtca tcactaataa acatcgctag agatgggcga ctttgatttt
      421 ccatttgttg attagtaaag gatataaata ctaacttgcc attgtcaaac cacatagatt
      481 tatatagtgg attaaattta aaccagtacg tcgttgcctc gccttgccgt actatttgta
      541 ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactatagt tattgccttt
      601 gggagaatca attctgacag cacataacat aggttcatct ttacagtcgt tatattgggt
      661 ttcatcaaac tcaactgcat agcacaataa aggcaatagg aaaaaaagga gattaattta
      721 ttactataca taaggcagcc tgactattca ttctaaaata aatccggttt ttaatatttg
      781 attctgctcc ggattatata cataataaat tttactgctc caaaatctga tattaaccct
      841 atggatttca taaatttttc agaaaaatag gctgtgcttg aacaatccgc cgactccggc
      901 tgtttgtcag ggaaaaggca attattttcg gttagtcagc agcctatcct attgaatcac
      961 ttaaagatat ggatgtttgt caacgccata tcaaaacacg gtcttttacc aatcgaattc
     1021 tgccgtcacc ggcgcgtggt cgctcggacg ctctaaagcg cgtgtatcca aatcgacacg
     1081 gacatccttc aaaacggccg ccatttcgga tgtaaccaat atatggtcga tacgcaggcc
     1141 cagtttgcgc tggaacatcg cgccgcgata gtcgaaccat gtgtaaaacg caccttcggg
     1201 atggatatgg cgcaaactgt cggtcaatcc caaatccagc aggttttgaa accactgccg
     1261 ttcgacggtc gaacagtgga ttttttcata ccatttttca gggtcgtaac agtccgcatc
     1321 ggcaggcgcg atattgaaat cgcccaacag caccagtttg ccgtggcgtg ccatttcatc
     1381 gcggacaaat tcggttaacg cggcaaacca ttgttcttta tatttgaatt tcgggctgtc
     1441 gagggcttcg ccgttgacgc aatagacatt gatgacgcgc acgccgccga cggttgccgc
     1501 aatcacgcgc cgttgcggat cgtccggcag cgcgggcagc ccgatatgca cgtcttccgg
     1561 cgcgttgcgg ctgacgattg ccacgccgtt gtaggttttc tgcccgctcc aaacacagtg
     1621 ccagcccatc atctgcaagg cggcagccgg aaatttgtcc tgatcgagtt tgagttcctg
     1681 caaaaccaaa atatcgggcg gattgtcggc aagcaggttt tgcacctgcg gcagccgcac
     1741 attgagcgaa ttgacgttcc aagtggtgat tttcataaca tttccgaaaa atgccgtctg
     1801 aaacgttcag acggcaaaag gaaaggattt taacactata ccgcgcgttt gttttccaaa
     1861 tcgcggattg tccgcagaat cgttgccgcg tcttcggaaa tcaggcggta ttcgataatg
     1921 cggtggtaac gcgtaaagtc caccagacct tccacgcgca gtcggctcaa atggccggaa
     1981 acggcggtgg ccggcaggga taaggatttt gccagttcgg cgatattgcg ttcgctgtcc
     2041 aacagttgga tcagtatcgc catacgttcg ggatgcgcca taagtttgag tatggtgtgg
     2101 agcggtattg tgttcatcgg tattcctaaa acctttgccg cccgccaagc ggattgacgg
     2161 gcgacagata tggatgattg tgattatctt tataaatctt attataaatc aaatatttat
     2221 ggatgattgt acaggattgt atgttccttt tcccactttt tttgcaaaca cgcgtaaagg
     2281 cgggctttca gacggcataa aactgccgcc cctaccctgc ctcaccctgt tgccgaacgg
     2341 aatcatccgc attgttcaaa agatgcctta caaccgccgc acgtcttgcc ctgccgatgc
     2401 ctccgtgcct tcgggcaaac aaaatacctg cctgaaatgc tgttttttct aaagcatcac
     2461 gaccttcacg aaaaatgccg tctgaagaat ctttcagacg gcatttcctc cttgaggcaa
     2521 taaccggatt acggttaggc ggttccttgt ctgacatatc agacaactgc cgacacccta
     2581 aaactcatgc caagaaatcc aaacctatgt ccaacgagcg gctgctgtgg gtcagatagc
     2641 cgagggcgat gccgtccacg ccggtttgcg ccacgcgctt caggcggtcg aagccgatgc
     2701 cgcccgatgc ttcgcaatag acggtgtggg ggtgggcggt ttgcgtgtgg cagcggtttg
     2761 ccgcttcttt cagggtttcg tcgtccatgt tgtccagcaa aatccgttcc gcgcccgctg
     2821 cgatggcttc gtccagttgt gccaacgtgt ccacttcgat ttctacgcag gtcaacggtc
     2881 cgaccgcctg ttttgcctgc cgaacggctt gggcgatgct gtcgcaatag gcgaggtggt
     2941 tgtctttgat gagtacggca tcgtccaaac ccatgcggtg gttcacgccg ccgcccgccc
     3001 tgacggcgta tttttgcagg acgcgcagca gggggatggt tttgcggctg cacacgatgt
     3061 ctgtaccgta tttggcgact tcggcaacgg cacgcgcggt ggcggtggag atgccgctca
     3121 agtgcgtgag gtagttgagc gcggtgcgtt cggcggcgag cagcgcgcgg gcgttgcctt
     3181 cgacggcggc aagcgtctga cctgcgcgga cgacttgccc gtctcggatt tcggcttgga
     3241 agcggacgga cggatccatc gtctgaaagg cgaggcgcgc caagtccatg ccggcgataa
     3301 caccgtcttc gcggctgacg aggaaaagtt tggcggtttt atcgggcgcg atgacggcgg
     3361 cggacgtaat gtcgccgcgc ctgcccaaat cttcgctcaa ggcttgttcc accatggggc
     3421 gcaacagggt gtcgggcagt gggaagaggg tgttttcaga cggcatgggg tttcctttgt
     3481 tgaaaaagat tgatgttgca ccgatgggca aaacttcgtt tgatgtcgtc tgaaaacggg
     3541 ctaacggctg cccagcggca atccgatgtc cgaaatcagc ctgtcgggca ggacgtcgtc
     3601 gcggaagcgg caaagctgcg cgggacggcc tttgctgccc gatacgccgg tatccgacgg
     3661 ctcgataagg ttttgctgct gaatctggcg gcggaagttt tgcttgtgca gcaatctgcc
     3721 gctgatggct tcgacgctgt tttgcagttg cagcaacgtg aattcgggcg gcatcagttc
     3781 gaaaatcacg gggcggtatt tgattttggc gcgcaggcgg gacagggcgg tcgccagtac
     3841 gcggcggtgg tcgtggcgca tgggctgccc cgtaagcgcg aagtcgaagt ttgcctgcgg
     3901 ctcggcggct tcctctatca ggccgctttc atacaacatt tcatagcgtt gcaaaacgta
     3961 ttcttccgac cagttttccg gttcgacgcc ccaacacaaa tgaatgcgct tgagccgctt
     4021 ttggcggact tcctccgtgt ccgccgagtt tgcccaaatg cgcaggcggc cgacgacggc
     4081 gtcgcgctgc ccgccgtcgg tgcgcaagtc ttcccacggg aaatagccgt agcagtcctg
     4141 ccatttcgca tcgggatgca ggatgctgtc ggctgcctcg cgcaccagcc ccaaatagct
     4201 gacgtacaag acgggcatgc cgtgttcgtt gcggcggtgg gtatcgacaa aggtgtaaag
     4261 ctgttccaca tagcccatag gctgcgaagt ttgcttggca acccacagct tcacgcccgc
     4321 ctgcagggaa ttgcgcagag gggagagcgg gccgttgggc aggagcgtgc cttgggcgac
     4381 ggtcaacacc cgcaggccgc cgtcggtaac ggcaatcaat acgggaacca gctcgacgat
     4441 gctttgcggc ggggcttcgg cttcggggta ggcatccatg ctcggctttc tgcgggtgga
     4501 aaaggcttta tt
//
LOCUS       DAWKST010000155         4506 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.155, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000155 DAWKST010000000
VERSION     DAWKST010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.155"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(49..3081)
                     /locus_tag="WJL87_001747"
     CDS             complement(49..3081)
                     /locus_tag="WJL87_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEZ7800154.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVSVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPNASDGYSDSGNGTEEAETEAAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNGAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKATVSAEARISR
                     LIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDETADIHIEEPAAPDAWVVE
                     PPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPSGFEQAQRSRIAETDHLADDVLNGGWQ
                     EETAAIADDGSEGAAERSSGQYLSETEAFGHDSRAVCPFEDVPSERPSCRVSDTEADE
                     GAFQSEETDAVYEHLPTTDLLLPPLFNPGATQTEEELLENSITIEEKLAEFKVKVKVV
                     DSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGLELPN
                     PKRQMIRLSEIFNSPEFAESKSKLTLALGQDIIGQPVVTDLGKAPHLLVAGTTGSGKS
                     VGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWC
                     VNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDNPEPLEKLPFIVV
                     VVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRI
                     AFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRVVEYL
                     KQFGEPDYVDDILSGGGSEELPGIGRSGDGETDPMYDEAVSVVLKTRKASISGVQRTL
                     RIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3246..3926
                     /locus_tag="WJL87_001748"
     CDS             3246..3926
                     /locus_tag="WJL87_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEZ7800155.1"
                     /translation="MIFQTVWFSDVVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAAFWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALAVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            3955..4314
                     /locus_tag="WJL87_001749"
     CDS             3955..4314
                     /locus_tag="WJL87_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ7800156.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPAAMSPATGNLFANWTGA
                     LLIGIFAETINHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGAAVK"
BASE COUNT          927 a         1318 c         1137 g         1124 t
ORIGIN      
        1 aaatgccgtc tgaaacagtc ttcagacggc atttccattt gcaaaaaatc aagcattgtc
       61 caaggggacg agaatcgtac ggttgccgtt gtgttccggt gcggacacaa tgccttccgc
      121 ctccatctgg tcaatcagac gcgcggcgcg gttgtagccg atgcgcaagg tgcgctgtac
      181 gcccgaaata ctggctttgc gcgttttcag gacaacggat acggcttcat catacatcgg
      241 atcggtttcg ccgtcgccgc tgcgcccgat gccgggcagc tcttcgctgc cgccgccgct
      301 caaaatatcg tcaacatagt ccggctcgcc aaactgtttc aaatattcga ccacgcggtg
      361 cacctcttca tccgaggcaa acgcgccgtg aacgcgctgc ggataggcag tacccggcgg
      421 caggaacagc atatcgccct gaccgagcag gttttccgcg cccatttggt cgagaatcgt
      481 gcggctgtcg attttgctgg acacttggaa cgcgatacgc gtcgggatgt tcgccttaat
      541 caaccctgta atcacatcga cgctggggcg ttgtgtggca agaatcagat ggatgcctgc
      601 cgcgcgggct ttttgggcga ggcgggcaat cagttcttcg attttcttgc ctgccgtcat
      661 catcaggtcg gcaaactcat caaccacgac cacgataaac ggcaattttt ccaaaggttc
      721 gggattgtcg ggcgtgaggc tgaacgggtt gccgattttc tccccccttg ctgcggcttc
      781 ggcgattttt tgattgaaac ccgcaagatt gcgcacgccc ataaagctca tcaggcggta
      841 gcgtttttcc atttcgttaa cacaccagtt cagcgcgttt gccgccagct tcatatcggt
      901 aacgacaggg gcgagcaggt gcgggatgcc ttcgtaaatg ctcaattcca gcattttcgg
      961 atcgatcata atcatacgca cgtcttccgg cgcggctttg aaaagcatag acagaatcat
     1021 cgcgttgaca cccaccgatt tgcccgaacc ggtcgtgccg gcaaccaaca aatgcggcgc
     1081 ttttcccaag tcggttacga cgggctgtcc gatgatgtcc tgaccgagcg cgagcgtcag
     1141 cttggatttg gattcggcaa actcgggtga attgaagatt tcgctcaggc gtatcatttg
     1201 gcgtttcggg ttcggaagtt ccaaacccat acaggttttg ccggggatgg tttcgacaac
     1261 gcggatggaa gccacgccga gcgaacgcgc caagtctttt tccagattca gaacggaatt
     1321 gccgcgcacg ccgacatcgg gttcgatttc ataacgcgta atcacggggc cggaataaga
     1381 atcgacaacc ttgaccttga ctttgaactc cgccaatttt tcttcgatgg tgatgctgtt
     1441 ttccaacagt tcttcttcgg tttgcgtcgc cccgggattg aacagcggcg gcagaagcag
     1501 gtcggttgtc ggcaggtgtt cgtataccgc atcggtttct tccgattgga acgccccttc
     1561 atccgcttcc gtatccgata cccggcagga cgggcgttca gacggcacat cttcaaacgg
     1621 acaaaccgcc cgactgtcat gcccgaacgc ttcggtttcc gacagatatt gcccgcttga
     1681 ccgctctgcc gcaccctcac tgccgtcatc cgcaatagcg gcggtttcct cctgccaacc
     1741 tccattcaaa acatcatcgg caagatggtc ggtttcggca atgcggctgc gttgcgcctg
     1801 ctcgaatcct gacggcggtt cataggtacg gttgtagatt tccgatacgg gaggcggcgg
     1861 cggaatatcg attgcgggca tgggaacttt cggcacttcg ggtggttcga ccacccaagc
     1921 atcgggcgcg gcaggttctt caatatggat atcggcagtt tcatcataaa ccggaccgcc
     1981 gtatcccaca gacgaaacgg tttccgtgaa aacattttcg gtttcagacg gcatttcgac
     2041 atcccgtttc ccgacaaccg tccgactttc cggaatcagg cgcgaaatcc gcgcctccgc
     2101 agaaaccgtt gccttgttcg gttctttcaa attgacggaa acgcggcggc gggcatcgcg
     2161 gacggctgtg ccgtctgaaa cggactgtcc ttgggaaaca tccggggatt ttgcctccgc
     2221 ctgccctgca tgacggcgga acggcgcatt ccgtgcattt tcggctttga ctgcagaaaa
     2281 ggcagacgga tgctgcttct caaaatacgg ctcaaatccg taatccgcag actcggaaaa
     2341 cgcttctttg tccgcatcga acatacggga atacgtgcgt tgaagcacag ggtcgtccaa
     2401 accgataatc tgcagccctt ccatcggggt atcggacacg cggactttgg ggaccgctgt
     2461 ttcgtttttc tcaaatgcat cgatatagcg ttttttcgtt tcccttaaag ccgcgccgtt
     2521 taaggcacgc gttgcttctt ccaaagtgat ttccttaaaa accggacgga cgggcgaaat
     2581 ttcgctttca gacggcatca acccttcagc aatactccgg tcgaatggga tgcggcggtt
     2641 gtcgattacg gcagttgcaa tgtcttccgt atcggcagcc tcttcctccg cagcttctgc
     2701 tgcttccgtt tccgcttctt ccgtcccgtt tccactgtct gaatatccgt cggatgcgtt
     2761 cggaacatcc gcacggtttg cagaagcagg cggaacaggc ctatccggtt cggattcgtg
     2821 cgccagatag tttcggcaga aacggacgac actgacgaac agccaataca ctgccgtttt
     2881 gacggcatgg aaaagcatca gggcaagttc gggaaaatcg ggcataccgt ctttgatttc
     2941 aggcagctcc gcctgttttt cccctttctt ttcctgccac gcagaaacct cgcgcatcca
     3001 ctcgcgttcg gattgtgcgc ggacaaaaaa caggccggca agcgcaagca acaaaataac
     3061 gatcaaaact atccaaaaca tatgccgttt cccgcaagac aaacgattaa acgggtttat
     3121 tttaacgtgt ttccatccca aaaaaaacaa ccagccggca attctgccga aaccgattcg
     3181 gctttcggtt tgcccccgcc gacagtataa tagccgtccc ttcccttaat ctttgcggca
     3241 ttgccatgat tttccaaaca gtctggtttt cagatgtggt actgtcggtt tcgtggattg
     3301 tccttatcct gatactggca gcttccgcgc cgtccgcatt ccgctcgctt gcccgctacc
     3361 gttccgccct tccactgtgt accgtaattt tttccgcagc atggtgtctg aacgcctctg
     3421 ccggcggcgg acagcttgcc cagatgaact accatctgct cgccgtcaat ctggttgcac
     3481 tgatggtggg cacttccgcc gccttttggc ttgccgcgct gctgatgctg ccctactgcc
     3541 tgctgtttgc cggttctgcc ggcgcatatc cgccgaacgc tttggtgctg attctgcccg
     3601 cgcttgccgt caaccgcctg tcgcgtatgc tggtcaaccg cctgccgccc aatattttca
     3661 tctttatttt cgtcaacggc tttctcgctt ccgccgccgg cattctgttg accgggctgg
     3721 tgctgaccgg tattttggat gctgcaaacg cttttccgtc cgaaatattg tggacgaccg
     3781 ccctgcccgt ctttattctg ctggcgtggg cggaagcttt tctcagcggc atttcaactg
     3841 ccatatttgt tgcactgaag ccccattgga tcaacacttt cgacgacaac cgctatctga
     3901 agtccgaccg cggcatatgg cggtagcctt cagacggcat agacaggcaa aaccatgctt
     3961 tccaacatca tccccctttc catcggtgcg gcactcggtg caacggcgcg ttggctgctc
     4021 aacctcgccg ttcctgccgc catgtctccc gccacgggca acctgtttgc caactggacg
     4081 ggcgcgctgc tcatcggcat ctttgccgaa accatcaatc acccgcaatg gaagctgctg
     4141 ctgattaccg gttttctcgg cagcctgacc acgctttccg gattttcact ggaaaccgta
     4201 accctgctcc aatcgaatcg tcctgcttca gcacttgcca atattttcct acacacggca
     4261 ggttcgctgc tgctgacttg gctcgggctg aagatagggg cggcagtcaa ataaccgcaa
     4321 caaattgctt cctttggttt tgacacaaac tatacaagtc ctaacatctt aatcatggcg
     4381 cagcatataa gcgtggattt gcagcccaat ctatactttc tgattctatt cattaagcca
     4441 ttatgcttat tcctcaatca cggtttccac caccgaatga atacttttag gcaattgcaa
     4501 aaaagc
//
LOCUS       DAWKST010000156         4495 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.156, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000156 DAWKST010000000
VERSION     DAWKST010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.156"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(104..1042)
                     /locus_tag="WJL87_001750"
     CDS             complement(104..1042)
                     /locus_tag="WJL87_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7800157.1"
                     /translation="MLELNGLCKRFGNKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIWLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAERLAMAALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVARLMGLPNTDDDRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMPHAGEISGNDTVRIHIEDREIVRFR"
     gene            complement(1051..1926)
                     /locus_tag="WJL87_001751"
     CDS             complement(1051..1926)
                     /locus_tag="WJL87_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ7800158.1"
                     /translation="MPTVRFTESVSKHDLDALFEWAKASYGAESCWKTLYLNGLPLGN
                     LSPEWAERVKKDWEAGCSESSDGIFLNADGWSDMGGRLQHLTRTWNKAGLLHGWRNEC
                     FDLTDGGGNILFSLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGRRSPHKAVDPDK
                     LDNTAAGGVSSGELPSETVCRESSEEAGLDKTLFPLIRPVSQLHSLRPVSWGVHNEIL
                     YVFDAVLPETFLPENQDGEVACFEKMDIGSLVATMLSGNMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(2108..2184)
                     /locus_tag="WJL87_001752"
     tRNA            complement(2108..2184)
                     /locus_tag="WJL87_001752"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2148..2150),aa:Met,seq:cat)
     regulatory      2334..2433
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2524..4425
                     /gene="thiC"
                     /locus_tag="WJL87_001753"
     CDS             2524..4425
                     /gene="thiC"
                     /locus_tag="WJL87_001753"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ7800159.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGAEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLNE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKSGGNVTQLHYARRGIITPE
                     MEFVAIRERLKLDELSQKSEYAKLLKQHAGQSFGANIPTHPDQITPEFVRREIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTADRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTSKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1086 a         1293 c         1184 g          932 t
ORIGIN      
        1 gttgtttcgg ataaattttg cagccctgat aaaaaatatg gctgctttgg taaaaaaatg
       61 ccgtctgaaa ggttttcaga cggcattttg tttttaagaa gcatcaacgg aagcggacga
      121 tttccctgtc ttcgatatgg atgcgtaccg tatcgtttcc cgatatttca ccggcgtgcg
      181 gcatatcgag gttcagccac aggatgccgt gttccggatg gaggacggac aggctgaacg
      241 attcgggcaa acaggtacgg gataatacgc ggcactccat gccgtcttgg tcgaaacgca
      301 ccgcatgttg cggaatatgg cggtcatcgt cggtattggg caaacccatc agtcgggcga
      361 cctgcacgcc ggcaggcgtt tgaaccaagg tttcgggcgt accgcattga aggattttcc
      421 cctcgtgcat gacggcgatt tcgtccgccg ccgtgcaggc ctcttcgggc gaatgcgtca
      481 ccaaaacggc agggatgccg cccttgcgga tacgttcggc agtcatgcgg cgcagccggt
      541 cgcgcaaatg cgtgtccaaa ctggaaaacg attcgtccaa cagcagcagg gaagggcgga
      601 caaccaaagc gcgcgccaac gccaaccgtt gcttctcgcc tccggaaagt ttttcaggct
      661 tgcggtgcgc ctcgttttcc agtccgactt cggcaagtgc cgccatggca aggcgttcgg
      721 cttcggcttt cggcattttt tgcattttca aaccgaatgc cgcattttcc agcgcactca
      781 tatggggaaa cagcgcgtaa tcttgaaaca tcagagaaat acggcgtttt tcgggcggca
      841 tacgggtaat gttttccccg ttcagccata tttccccacc gtccggccgg acaatccccg
      901 caatcatatt cagcagggtg gattttccgc atcccgaccg ccccaaaacg gcgagtattt
      961 tgccgcgccc gacagtcagg cagatgttgt cggcgacggt tttattgccg aagcgtttgc
     1021 agagtccgtt cagttcaagc atggcgcatc ctataaacgt atgccgtcca gccactcgga
     1081 cagcggatgg gcggcatcaa tcagaccgta acggcaaaac gcgtccagcg taaccagttg
     1141 cgcgtcgtgc atcatgtttc ccgacaacat ggtggcaacc aagctgccga tgtccatttt
     1201 ctcaaaacac gcaacctcac cgtcctgatt ttcaggtagg aaggtttcgg gcaggacggc
     1261 atcgaataca tacaggattt cattgtgcac accccagctg acggggcgca ggctgtgcag
     1321 ctgcgatacc gggcggatga gcggaaacag cgttttatcc aaaccggctt cttcgctgct
     1381 ttcgcgacac acggtttcag acggcaattc accgctggaa acaccgccgg cggcagtatt
     1441 gtcgagtttg tcgggatcga ctgctttgtg cggactgcgc ctgcctatcc agaaatgcca
     1501 tcggccgtcc gattcggtca gaccgttgag atggacggcg cggctgagta gtccgaacgg
     1561 gcggaaagcg gcgcgttcaa gtgagaacaa gatattgccg ccgccgtcgg tcaggtcgaa
     1621 acactcgttg cgccatccgt gaagcagccc cgccttgttc catgtgcggg tgaggtgctg
     1681 caagcggccg cccatatctg accagccgtc cgcattcaga aaaatgccgt ctgaagactc
     1741 cgagcagcct gcctcccagt cttttttgac gcgctccgcc cattccggcg acaggttgcc
     1801 caaaggcagg ccgttcagat acagtgtttt ccagcaactt tctgcaccgt aacttgcttt
     1861 tgcccactcg aacagggcat caaggtcgtg tttgctgacg gattcggtaa aacggacggt
     1921 cggcatagtc ggctttcttg aagacaatgc ggaagattgt agccaaactg cctgaatttg
     1981 caaccgcaaa cagacggcgg gctgccgttc cgatattgcg gcaggaagga aaatccggca
     2041 aaaaacggag gcggcaggaa acaggcaaaa caaaacccct cggcattgaa ccgaggggtt
     2101 tgatatttgg ttgcgggagc aggattcgaa cctacgacct tcgggttatg agcccgacga
     2161 gctaccatgc tgctccatcc cgcgtcagaa gatgaaacta tacggcagat tttttatgtt
     2221 gtcaaacatt atttccgcga aaaatcataa aattttgccg tccggcggtt tatgcctgat
     2281 ttgaaatatc attttcttta caaaagttca tgttcgtgat ttaattttgg ttaacatcga
     2341 aacaggggtg ctgcctgatg ttcaggcggc tgagaaatac cctttacacc cgatcgggat
     2401 aatacctgcg tggggagttt tcacggattc tgtctttcag acagcattgg ttttcaaatg
     2461 ccgtctgaaa acgcaaaacg ctcctgtttc tttattctaa acgagaaaac aggagcattt
     2521 tttatgacta cgccaaaaaa aaccgccaaa acttccggca acgaagcgcg cgaacttgcc
     2581 gacttgagcg aagacatcgg tatccgcttc aaatatccga actcggaacg cgtgtatctg
     2641 caaggcagcc gcgacgacat ccgcgtgcct ttgcgtgaaa tccgtcagga cgacacctat
     2701 acggcgcaag gcgcggaagc caacccgccg attcccgtct atgacaccag cggcgtgtac
     2761 ggcgatccag cggcacacat cgacctgaaa caaggcctgc cgcacatccg caccgcatgg
     2821 ctgaacgaac gcggtgatac cgaaatcctg cccaagctct ccagcgaata cggcatcgaa
     2881 cgcgcacacg atccgaaaac cgcccatctg cgcttcaacc aaatcacccg cccgcgccgc
     2941 gcgaaaagcg gcggcaacgt tacccagctt cactacgcgc gccgcggcat catcacgccc
     3001 gaaatggagt ttgtcgccat acgcgaacgt ttaaaattag acgaattgtc ccaaaaatcg
     3061 gaatacgcca aactcttgaa acagcacgca gggcaaagtt tcggcgcgaa tatcccgacc
     3121 catcccgacc aaatcacgcc tgaattcgtg cgccgagaaa ttgccgccgg acgcgcgatt
     3181 atccccgcca acatcaacca ccccgaactc gaaccgatga ttatcggccg caacttccgc
     3241 gtcaaaatca acggcaactt gggcaattcc gccgtcactt ccagcctgac cgaagaagtc
     3301 gaaaaaatgg tgtggtcgct gcgttggggc gcggacacga ttatggattt gtccaccggc
     3361 gcgcacatcc atgaaacgcg cgaatggatt atccgcaacg cgcccgtccc catcggcacc
     3421 gtgccgattt accaagcgtt ggaaaaaacc ggcggcattg ccgaagattt gacttgggat
     3481 ttgttccgcg acaccttaat cgagcaggcg gaacaaggcg tggactattt caccatacac
     3541 gcaggcgtgt tgctgcgtta tgtgccgatg actgccgacc gcctcaccgg catcgtatcg
     3601 cgcggcggtt cgattatggc gaaatggtgt ttggcacatc atcgggaaaa cttcctttac
     3661 acgcatttcg acgaaatctg cgaaattatg aaggcgtatg acgtatcgtt cagcctcggc
     3721 gacggtttgc gtcccggctg cattgccgat gccaacgacg aatcccaatt cgccgagctg
     3781 cacaccttgg gcgaattgac ctccaaagcg tggaaacacg acgtacaggt gatgatcgaa
     3841 ggtcccggcc atgtgccgct gcaacgcgtc aaagaaaata tgaccgaaga gttgcaacac
     3901 tgttttgaag caccgtttta caccctcggc ccgctcgtta ccgacatcgc ccccggctac
     3961 gaccacatca cctcgggcat aggcgctgcc aatatcggct ggtacggcac ggcgatgctc
     4021 tgttacgtta cccccaaaga acatttggga ctgcccgaca aagaagacgt gcgtaccggc
     4081 atcatcacct acaaactcgc cgcccacgcc gccgacctcg ccaaaggctg gccgggcgca
     4141 cagttgcgcg acaacgccct gagcaaggcg cgtttcgagt tccgctggcg cgaccagttc
     4201 cgcctaagcc tcgaccccga acgcgccgaa agcttccacg acgaaaccct gcccgccgaa
     4261 ggcgcgaaaa tcgcccactt ctgttcgatg tgcggcccca aattctgctc gatgaaaatc
     4321 acgcaggaag tgcgcgacta cgccgacaag caaaaagccc aacggcaggg catggaggaa
     4381 aaagcggtcg agttcgtcaa aaaaggagcg aagatttaca gctaaaacgt caagcaaaaa
     4441 atgccgtctg aaacccggaa aaaaggcttc agacggtatt ctttcgcttg tgaaa
//
LOCUS       DAWKST010000157         4488 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.157, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000157 DAWKST010000000
VERSION     DAWKST010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.157"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            114..422
                     /locus_tag="WJL87_001754"
     CDS             114..422
                     /locus_tag="WJL87_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800160.1"
                     /translation="MEAQSEIHINLEVLLNGNSRASIEAQSLMQEQAEEIIDDETVNQ
                     GFSITTQQGEVIQPSDVKLSKSVRIGRYDEANSLLPDSAFTAISEYFLELQSRNLTEQ
                     "
     gene            419..901
                     /locus_tag="WJL87_001755"
     CDS             419..901
                     /locus_tag="WJL87_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800161.1"
                     /translation="MKKYLPLLRFLLIACISAFLAWKGQPFVHGNEKAVDLIINVFAI
                     LAGFLIAIMTLFSDMRFDEDANWRQIQIREGVQEQRYIKHSLLFYTYLAVLVCVFIVI
                     LLAHKEEYKNGPAIFWLERSYLFLACISIFYSVFLPGNLIKSRKEEFKKLMEKKKPKI
                     "
     gene            991..1494
                     /locus_tag="WJL87_001756"
     CDS             991..1494
                     /locus_tag="WJL87_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ7800162.1"
                     /translation="MSDEFNRFIERVLSHEGGYVNHPQDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAIGIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAAG
                     VPDDGIIGEISLKAINSLPENDLLLRFNAERLVFYTKLGTFTSFGKGWVRRVAQNLIH
                     ASADNTD"
     gene            1606..1944
                     /locus_tag="WJL87_001757"
     CDS             1606..1944
                     /locus_tag="WJL87_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800163.1"
                     /translation="MLDASPPQERGSRRETRSQKKASQITAFFRWLGGLVSNPATGKI
                     SHTKLWTNVAAASMTYKFSQTADAPEWLWWAYGALVGGYALIKRGIAAVPQLAEIKKS
                     ANAEEWSGND"
     gene            1937..2347
                     /locus_tag="WJL87_001758"
     CDS             1937..2347
                     /locus_tag="WJL87_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ7800164.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLTAEMAALKKPDALIHGKEVRQKERNSSQG"
     gene            2301..3101
                     /locus_tag="WJL87_001759"
     CDS             2301..3101
                     /locus_tag="WJL87_001759"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ7800165.1"
                     /translation="MGKKCGRKNATRRRVKAMPSVELLLEIVGLPRGTFYYQLAVQSA
                     EDKYADLKQHIHDIYRRHKGRYGYRRIAAAILHAGTPVNHKKVSRLMAKTGLKAVIRR
                     RKYRSFKGEVGKIAPNILRRCFHAEKPNEKWVTDVTEFNVGGEKIYLSPIMDLFNGEI
                     VSYRIQIRPTFDLAGEILKGAPEKPGSSEKPILHSDQGWQYQMFFYSGLNLNQDKAMK
                     PQTVQIVRQGEATPYWFKFNPLYNNDRIKLKLKGLSLVQYRIQSLKAA"
     gene            complement(3420..3782)
                     /gene="ssrA"
                     /locus_tag="WJL87_001760"
     tmRNA           complement(3420..3782)
                     /gene="ssrA"
                     /locus_tag="WJL87_001760"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(3845..4450)
                     /locus_tag="WJL87_001761"
     CDS             complement(3845..4450)
                     /locus_tag="WJL87_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEZ7800166.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QLSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
BASE COUNT         1194 a          996 c         1107 g         1191 t
ORIGIN      
        1 ttgcacttga aatccgaatc caagacagac tgtatcaaga tagccgttca accattgcta
       61 aagcattagg gaaagttggt agtgtattta caagcgaatt gagtgatgag gaattggaag
      121 cacaatccga aattcatatc aatttagaag tcttgctaaa cggtaattcg agagccagta
      181 ttgaagcgca atcgctgatg caggagcagg cagaggaaat tattgatgat gaaacggtta
      241 atcaaggatt ttccattacc acgcaacaag gtgaagtgat tcagccctct gatgtaaaac
      301 tgtctaaatc tgttagaata ggacgatacg atgaagccaa ctcgttactc cctgactcgg
      361 catttacagc aatcagtgaa tatttcctag aactccaaag cagaaactta actgaacaat
      421 gaaaaaatat ctgcccttac tacgtttttt gttgattgct tgtatatcag catttttagc
      481 atggaaaggg caaccttttg ttcatgggaa cgagaaggcc gttgatttaa ttattaacgt
      541 atttgcaatc ttagccggtt ttttaattgc cattatgaca ttgtttagtg atatgaggtt
      601 tgatgaagat gcaaattggc gacaaattca aattcgtgaa ggcgtacaag aacaacggta
      661 tataaaacat tccttacttt tttacacata tcttgccgta ttagtttgtg tttttattgt
      721 catcttgttg gctcataaag aagaatacaa aaatggacca gccatttttt ggcttgaacg
      781 gagctattta tttttagcct gtatttccat tttttattct gtattcttac ctgggaattt
      841 aattaaaagc cgaaaagaag agtttaaaaa attaatggaa aagaagaaac ccaaaatcta
      901 gaacgagttt taaccccctt taaaatccca ttcagacgac cttttctaaa atccctgtat
      961 tgatttttaa ttcaatacag ggatttttcc atgtcagacg aattcaaccg atttatcgaa
     1021 cgcgtcctct cccacgaggg cggttacgtc aaccaccctc aagaccccgg cggcgaaacc
     1081 aattggggca tcactaagcg caccgcccag gcaaacggct acaacggctc catgcgtgcc
     1141 atgacgcgtg aacaggctat cggcatttac cgaaaggcgt tttgggagcg ttaccgcgcc
     1201 gaccaaatgc cggaagcggt cgcgttccaa ttttttgatg cctgcgtcaa ccacggttac
     1261 ggcaatgccg cccgtatgct ccaacgcgcc gcaggcgtgc cggacgatgg aatcatcggc
     1321 gaaatcagcc tcaaagccat caattccctc cccgaaaacg accttttatt gcggttcaac
     1381 gccgagcgtc tggtctttta taccaagctg ggtacgttca cctctttcgg caagggctgg
     1441 gtacgccgtg tggcgcaaaa cctgattcac gcgtctgcag ataacactga ttaaagggag
     1501 acaaaccatg tcaaaaaagt cactcatcgc cctaatgacc gcagccatgc agcccgattt
     1561 cagccacagc gacctaggca ttcgctacgc catgccgact cagggatgtt ggacgcaagc
     1621 ccaccgcaag agcggggtag ccgccgcgaa acgcgcagcc aaaaaaaagc gtcgcaaata
     1681 actgcctttt tccgatggct gggcggcttg gtctctaatc cggccacagg gaaaatcagc
     1741 cataccaaac tatggacaaa cgtggcagcc gcttctatga cctataagtt ctctcaaaca
     1801 gcagatgcgc cggaatggct ctggtgggct tatggcgcat tggtcggcgg gtatgcatta
     1861 atcaaacgcg gcatcgccgc cgtaccgcag ttggcagaaa tcaaaaagtc cgcgaatgcg
     1921 gaagaatgga gcggcaatga ttgaatttgt ccgagccaaa aaacggctgc tttgggcatt
     1981 tgtgcttttg cttgtgtgga cgtgcggtta ccgatacgcc gccgacaagg ctgaagcgga
     2041 acaaaccgcc ctgattgcca cctatcggca ttcttctatg gttgcggcgg aacaatatgc
     2101 cttgcagctt aaaaaagcgc aggacgaaag gcagcggtgg tacgactttt cccaaaaaca
     2161 aggaagaaag cccgtgaaaa aacagtatcc gccgcaaacg aaaaaagccg gctatctgaa
     2221 aaccaaggaa gaactgcttg cggaattggc ttgccttaca gcggaaatgg ctgccctaaa
     2281 aaagcccgat gccttaatcc atgggaaaga agtgcggcag aaagaacgca actcgtcgca
     2341 gggttaaggc aatgccatcc gttgaactgc tgttggagat tgtcggactg ccacgcggca
     2401 ccttctatta ccaattggcc gtccaatcgg cagaagacaa atatgccgat ttgaaacagc
     2461 atatccatga tatttatcga cgacataagg gaagatacgg ctaccggagg attgcagcag
     2521 ccatccttca tgcaggaaca ccggtcaatc acaagaaagt cagccgtctg atggcgaaga
     2581 cggggctgaa ggcagtgata cggcggcgca aataccgctc gttcaaagga gaagtcggca
     2641 aaattgcgcc gaatatcctg cgacgctgtt tccatgcaga aaagccgaat gagaaatggg
     2701 taacggacgt taccgagttc aatgtaggcg gagaaaagat atacctttct ccgattatgg
     2761 atttgtttaa cggggaaatc gtcagttacc gtattcaaat ccgcccgact ttcgatttgg
     2821 ccggcgagat actgaaaggt gcgccggaga aaccgggatc gtctgaaaag ccgatactgc
     2881 attcggatca aggttggcaa tatcagatgt ttttttatag tggattaaat ttaaatcagg
     2941 acaaggcgat gaagccgcag acagtacaaa tagtacggca aggcgaggca acgccgtact
     3001 ggtttaaatt taatccacta tacaacaacg atagaatcaa gttgaaatta aaagggctga
     3061 gccttgttca gtacagaatt cagtccctga aagccgcttg attaaactgt ccgacttttt
     3121 ggggtcagtt cggcttcggc atttttttat ccgtttgggg gtaacttgtt tggaaagctg
     3181 caagcttata aataaaggat tacatttaag ttttgggtaa cctttttaaa aaaatgcgtg
     3241 atgacttttg catttttaag gcgttttttg gggtaattcg tgaaaagtta ccccaaaagt
     3301 taccccataa atggcgaaaa ctcaagcata cgccagcata ttcaaggcaa caaaaaagcc
     3361 ttgaaactgt tgaagttcaa ggcttttttg tgttgcagga tgctgctgaa aatagggtat
     3421 ggtggaggcg gggggaatcg aacccccgtc cgaaagtcct ctacaaagcg ttctacatac
     3481 ttagttgtgt ctatttgaaa atcttatttc catcatgccg accaacaggc cttttggaaa
     3541 ccagttacct taaatctcat ttcctgccaa gtaacccggt aggaaaccag tcaatgtaag
     3601 atgacgttgc ggtggctttc gccacacagc ccattgaccg actgctgcaa cggctagcct
     3661 taagcggcta aagcgtaagt ttcgtcgttt gcgactattt gaattcagtg ttttacggga
     3721 atctgagacc ccggtatgcc cgcatctgct tcgcaacccc cgtcgaaacc aaggtcgccc
     3781 ccaaaaatgg tttgcaaatt atacggatat tgtgcggtgc tgccaagtct gtcggagaaa
     3841 tttgtcagtc ttgctgcctt aatttgcgtt tgagcaggat gcggacgcag ccgtcgttgc
     3901 cttcccgggg ttcggcgtag gcgagtacgt cggggtgttg catcagccag tttcgggtca
     3961 tatttttcag aacgggtttg tagcctttgg aacctaatcc gctgccgtgg atgatttcgc
     4021 cgcatacgcc gcgtttttgg gtgaatgcga tgaattcgtt gaggactttt tgggcttctt
     4081 cctgtgtgta gccgtgcagg tcgacatcgg taacgacggg atagtatccg tttttcaggc
     4141 gttggatgtc gttttttccc tgtccgtttt tgctgaagct ggcgggcggg tcgttgtatg
     4201 tgctgccgat gtagaagtag ttttcttcgt cggcgcggtt gtctttggga cggactttga
     4261 tgggggtttt gtcgggcggc gcataatatt gctgccggtt ttttaatggg gagagttgtc
     4321 cgactgcttg tgaaaaatcg aaatcctgtt cttgtttttg cttgtttttt tcggctgcct
     4381 gtttttctgc cgcttctttt tgggcttgtt tgcccagttg tttgaggatg ttttggaagt
     4441 cggtattcat attttttcct gttatttgtc cgatggctgt ttcgggcg
//
LOCUS       DAWKST010000158         4482 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.158, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000158 DAWKST010000000
VERSION     DAWKST010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.158"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            126..1973
                     /locus_tag="WJL87_001762"
     CDS             126..1973
                     /locus_tag="WJL87_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ7800167.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTHAPSSKTLADNIETAGLSAS
                     VPTRPAEPEGKTLADYSSYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKSL
                     GARKQWTLFAQEYAKLEPAGRAQEVECYADSSRNGYTRAAELVKNTGKLPSGCTKLLE
                     QAAASGLLDGNNAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIGK
                     EARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQSLNVPAALDYYGKVADRRQLT
                     DDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKLY
                     KQAAATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAIKRALVLFRNSRT
                     AGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTLR
                     YISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVMGAQSGVGAQGLMQVMPATAREIA
                     GKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADIPL
                     EGAVYAETIPFSETRDYVKKVMTNAAYYASLFGAPHIPLKQRMGIVPAR"
     gene            2262..2474
                     /locus_tag="WJL87_001763"
     CDS             2262..2474
                     /locus_tag="WJL87_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ7800168.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            2606..3052
                     /locus_tag="WJL87_001764"
     CDS             2606..3052
                     /locus_tag="WJL87_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ7800169.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLVDKENAEIEVLHRYLPQMLSAGEIRTA
                     VEAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(3377..3769)
                     /locus_tag="WJL87_001765"
     CDS             complement(3377..3769)
                     /locus_tag="WJL87_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ7800170.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(3840..4445)
                     /locus_tag="WJL87_001766"
     CDS             complement(3840..4445)
                     /locus_tag="WJL87_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ7800171.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
BASE COUNT         1084 a         1251 c         1237 g          910 t
ORIGIN      
        1 attatgtcgg aaacattcca aaggcggtgc agtttcggca tataattcgg gcaaacgcct
       61 gttcagacgg cattttgtct tttccaaccc tgaccgttca gggttccgat tcttaaggaa
      121 atccgatgta cctaccctct atgaagcatt ccctgccgct gctggcggcc ctggtgcttg
      181 ccgcgtgttc ttcgacacac gcgcccagca gcaagaccct ggcagacaat atagaaactg
      241 ccggtctttc ggcaagcgtt cccacccgcc ctgccgaacc cgaaggaaaa acgctggcag
      301 attacagcag ctacccgtcc gcactggatg cagtgaaaca gaaaaacgat gccgccgtcg
      361 ccgcctattt ggaaaacgcc ggcgacagtg cgatggcgga aaatgtccgc aacgagtggc
      421 tgaagtcttt gggcgcacgc aaacagtgga cgctgtttgc acaggaatac gccaaactcg
      481 aaccggcagg gcgcgcccaa gaagtcgaat gctacgccga ttcgagccgc aacggctata
      541 cgcgtgccgc cgaactggtc aaaaatacgg gcaaactgcc ttcgggctgc accaaactgt
      601 tggaacaggc agccgcatcc ggcttgttgg acggcaacaa tgcctggagg cgcgttcgcg
      661 gactgctggc cggccgccaa accacagatg cacgcaacct tgccgccgca ttgggcagcc
      721 cgtttgacgg cggtacacaa ggttcgcgcg aatatgccct gttgaacgtc atcggcaaag
      781 aggcgcgtaa atcgccgaat gccgccgccc tgctgtccga aatggaaagc ggtttaagcc
      841 tcgaacaacg cagtttcgcg tggggcgtat tggggcatta tcagtcgcaa agcctcaatg
      901 tgcctgccgc cttggactat tacggcaagg ttgccgaccg ccgccaactg accgacgacc
      961 aaatcgagtg gtacgcccgc gccgccttgc gcgcccgacg ttgggacgag ctggcctccg
     1021 ttatctcgca tatgcccgaa aaactgcaaa aaagcccgac ctggctctac tggctggcac
     1081 gcagtcgcgc cgcaacgggc aacacgcaag aggcggaaaa actttacaaa caggcagcag
     1141 cgacgggcag gaatttttat gcggtgctgg cgggggaaga gttggggcgc aaaatcgata
     1201 cgcgcaacaa tgtgcccgat gccggcaaaa acagcgtcct ccgtatggcg gaagacggcg
     1261 cgattaagcg cgcgctggtg ctgttccgaa acagccgaac cgccggcgat gcgaaaatgc
     1321 gccgtcaggc tcaggcggaa tggcgttttg ccacacgcgg ctttgacgaa gacaagctgc
     1381 tgaccgccgc gcaaaccgcg ttcgaccacg gtttttacga tatggcggtc aacagcgcgg
     1441 aacgtaccga ccgcaaactc aactacacat tgcgctatat ttcgccgttt aaagacacgg
     1501 taatccgcca cgcgcaaaat gttaatgtcg atccagcgtg ggtttacggg ctgattcgtc
     1561 aggaaagccg cttcgttatg ggcgcgcaat ccggcgtagg cgcgcagggg ctgatgcagg
     1621 ttatgcctgc caccgcgcgc gaaatcgccg gcaaaatcgg tatggatgcc gcacaacttt
     1681 acaccgccga cggtaatatc cgtatgggga cgtggtatat ggcggacacc aaacgccgcc
     1741 tgcaaaacaa cgaagtcctc gccaccgcag gctataacgc cggccccgga agggcgcgcc
     1801 gatggcaggc ggatatcccc cttgagggtg cggtatatgc cgaaaccatc ccgttttccg
     1861 aaacgcgaga ctatgtcaaa aaagtgatga ccaatgccgc ctactacgcc agcctcttcg
     1921 gtgcgccgca catcccgctc aaacagcgta tgggcattgt ccccgcacgc tgacgtatcg
     1981 atgccgtctg aaacccgccc ggtctttcag acggcatttt tatcccgaac ggcattgacg
     2041 gcgaaccata aatataagac aatccggaaa ttgtttttcc tgctttttca agcagcttga
     2101 cacggcacaa gccgacccgt taggaggtga tgtttccgtc acggcgcgta tcccgccgcc
     2161 gcaaggcaca gcgatacggt aaaccttcaa caccgtctgc cctacccttt ccaccgatat
     2221 gatgggcaga tgcaacaacc gaatttatta aaggaaataa aatgcctgca atccgcgtaa
     2281 aagagaatga accatttgaa gtcgctatgc gccgtttcaa acgcgccgta gaaaaaaccg
     2341 gcctgctgac cgagctgcgc gcccgcgaag cctacgaaaa accgactacc gaacgcaaac
     2401 gcaaaaaagc ggcagccgta aaacgcctgc aaaaacgcct gcgcagccaa caactgccgc
     2461 ccaaaatgta ctaaacgttc aagtacagat tacaggtcag ccctgtgata tgaggacaca
     2521 ccgcaagacc tgctctgcgg tgtgttttgc ttttcagacg gcatcgaaac ccgccgtttc
     2581 catccgacat cccagcgagg acattatgag cctgaaaatc cgccttaccg aagacatgaa
     2641 aaccgcgatg cgcgccaaag accaagtttc cctcggcacc atccgcctca tcaacgccgc
     2701 cgtcaaacag tttgaagtgg acgaacgcac cgaagccgac gatgccaaaa tcaccgccat
     2761 cctgaccaaa atggtcaaac agcgcaaaga cagcgcgaaa atctacactg aagccggccg
     2821 ccaggatttg gtagacaaag aaaacgccga aatcgaggta ctgcaccgct accttcccca
     2881 aatgctctct gccggcgaaa tccgtactgc ggtcgaagcc gccgttgccg aaaccggcgc
     2941 ggcaggtatg gcggatatgg gcaaagtgat gggcgtattg aaaacccgac ttgccggcaa
     3001 agccgatatg ggcgaagtca acaaaatcct gaaagccgta ctgaccgcct gattgcccga
     3061 atatcggaca aaatgccgtc tgaagcccgt atcgcaggtt cagacggcat tttcatggtt
     3121 tgatgatatt tataaaaatt ttgcaacacg ctcatgcaca gcaactgccg ctgctcgttg
     3181 gcgttgccgc tctatcgctt ggttagctgc accataattg cactctaatg ctgatgcaat
     3241 aacgtcccaa tatcgaattg gcaggggcaa cacggtttcg atacaccgaa acgggttttg
     3301 ccgataaacg gattccgttt gcgccccatc ggacaaaatg ccgtctgaaa cacgattccg
     3361 ttcagacggc atagatttat ttgaccaatt tcaagccttt tttggcgggc cggggcgcgg
     3421 tttcggcaga ggtgttttca ggcggcgtgt cggggcgata cgcttccaat tcaaacccca
     3481 taccttctcc ggtctcccgt gcgaaaaggc tgaggacgtg tccgacaggt atccatatat
     3541 cgtgcgcctg tccgccgaag cgggcggaaa aactgatcca atcgttgtcg atttgaaggt
     3601 tttgcgtggc ggtcgcgccg atgttgagca taatttcgtt gtcgcggaca tactgcatag
     3661 gaacgcgcgt gtgttcgttg acccagacaa ggatgtgcgg tgtgaggctg ttgtcgctgc
     3721 accattcgca gagggcgcgg aggatgtagg gtttggtgga agtgggcata atggttccgt
     3781 gttgtacgcc aaaataggaa aatgcctgca aaacagtggg ttttgcaagc atttcggact
     3841 tatttgcgca tggctttttc ggcgggtgtc agtgcttcga taaaggcttc gcgctggaag
     3901 atgcgctcgg cgtatttgag cagcggcgcg gcacttttgc ccagtttgac atcgtagtgg
     3961 tcgagccgcc acagcagcgg agcaagggcg acatcaatca tagaaaaatc ttcgccgagg
     4021 atgtatttgc ttttgctgaa cgaaggggca agcatggtca gaccgttgcc gatggcttcg
     4081 cgcgcttttg cctgttcctt gttggtggcg gcggggtttt ccaaaacttg gacgtggttg
     4141 aacaattctt tttccatacg gtacagcacc agccggcccc tgccgcgcat aacgggatca
     4201 ccgggcataa gctgcggatg ggggaagcgt tcgtcgatgt attcgttgat gatgttggac
     4261 tcgtgcagca ccaaatcgcg ctcgaccagt acggggacct ggttgtacgg gttcatgacg
     4321 gcgaggtctt cgggtttgtt gtaaatatcg acgtctttga tttcaaaatc catacctttt
     4381 tcgtacaaaa cgaagcggca gcggtggctg aaggggcagg taatgccgga atagagggtc
     4441 atcataataa ttgtcgctcc tgtgtgatgc ctacaaaacg gc
//
LOCUS       DAWKST010000159         4455 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.159, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000159 DAWKST010000000
VERSION     DAWKST010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.159"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            153..1058
                     /locus_tag="WJL87_001767"
     CDS             153..1058
                     /locus_tag="WJL87_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ7800172.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFRQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFV
                     SGLVSGLMLALSMVFVRKTHEIETVALFPSMMILSLGGAVSLVVPALLMDGGALYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQSEP"
     gene            1104..1253
                     /locus_tag="WJL87_001768"
     CDS             1104..1253
                     /locus_tag="WJL87_001768"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800173.1"
                     /translation="MNWQAVKTGKTKHASNGAERKMPSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            complement(1472..2491)
                     /gene="lepB"
                     /locus_tag="WJL87_001769"
     CDS             complement(1472..2491)
                     /gene="lepB"
                     /locus_tag="WJL87_001769"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ7800174.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNIFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRDKILTVNGKPTSDIPNGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(2634..4427)
                     /gene="lepA"
                     /locus_tag="WJL87_001770"
     CDS             complement(2634..4427)
                     /gene="lepA"
                     /locus_tag="WJL87_001770"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ7800175.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
BASE COUNT          978 a         1127 c         1215 g         1135 t
ORIGIN      
        1 gggttttatc cggcaggacg gttttttgcc ccaacggaaa atagtcggcc tgcccgtaaa
       61 atcagccgtt tgtccgggtg cagccggggc tttgggcttc agacggcata ttttcggaat
      121 ggcggcattc ttgccgtcgg cgcggcagcc gtatggggaa gggaggggat attgtggtcg
      181 gtaacggcaa aaaatatgcc gcaccattgc tggtgctggg ttgcgtggtg ttcggtctgg
      241 gcagtctgat tgtcagatcc gtccccgtcg gttcgtatgc aatcgcattt tggcggttgc
      301 tgatttcggt gttcgtattt tggtttttag cacggttttt caggcaaaaa ttcccaaaaa
      361 acaggaaaac cgtccgatat gccctgacgg cgggcgtgtt tctcgctttc gatttggcgt
      421 tgtggcacga aagcatacac gcggtcgggc cgggtatttc caccctgctc aacagcctgc
      481 aaatcttttt cttgtcggca atcggtgttt tctttttcgg cgagcgtttg agcgggctga
      541 aaaaggcagg cttaatatcg gcagttgccg gcgtggcgat gattgccggt gcggaattcg
      601 gctacaacgg taatgcggtt tggggattcg tcagcggttt ggtatcggga ctgatgctcg
      661 ccctgtcgat ggtgttcgtc cgcaaaaccc atgaaatcga gacggtggcg cttttccctt
      721 caatgatgat tttgagtttg ggcggcgcgg tatcgctggt tgttccggca ttgctgatgg
      781 atggcggcgc gctttatccg acgacttgga aagatgcggg tttggtgctt gtgtacggcg
      841 tggtgatgca gtgcttcgcg tgggcgatgg ttgcctatgc gattccgctg ctttcgctgt
      901 cgctgacggg gctgctgctt ttgtccgaac cggttgccgc cctgttcatc gattatttcg
      961 ggttgggcaa accgattgaa ggcgtgcagt gggcaggggt ggcgctgacg ctttcggcaa
     1021 tttacctcgg ttcgctgaaa cagcagtccg agccttgact ccgtccggca tcagattttc
     1081 tgccgtggca aacctgcgta cgattgaact ggcaggcagt caaaaccggc aagaccaaac
     1141 acgccagcaa cggcgcggag cggaaaatgc cgtctgaagc cctttcagac ggcatcgggg
     1201 cgggcagaat caaaaaagcc gcctccgctg gaatggcaat gcaactaaaa taatgtccgt
     1261 atttccatat gccgcccaca ccgtcagtcg gacgaaatgc cctttattca atgggatggg
     1321 tttccacaca aacggaaata tagtggatta aatttaatcc actatattat ccccgaggct
     1381 gccggcaggg aaacaaggcg tttgatgata aaaatgccgt ctgaaacagg gttcggatgg
     1441 catttttgat gttccggcgt aattttccgg cctaacggat tgccgtaccg gccctgccga
     1501 aatcgccgaa gttcatcaaa atgaacattg ccttgccgac aaccagcttg tcatccacaa
     1561 atccccagta gcgcgaatcg gcactgttgt cgcggttgtc gcccatagcg aaatagcgtc
     1621 cttcgggaac tttgcacacg aaaccgctgc cgtcgtcggc atattggcag tgttccaaac
     1681 cgctttgctc tatggaatat ccgttttcag acataatatc ggaggtatat ttgcccaata
     1741 cgggcaggga aacggccggc tgtccttctt ttttcagaat attgaaggat ttgccgtcca
     1801 aaccgctgcg gaacatatcc gtgttgtgga tttcggaagg gtcggtatcg tcgggataac
     1861 ggtatgtgcc gttaggaata tcggaagtgg gtttgccatt taccgtcaaa atcttatccc
     1921 gatattcgac cacatcgccc ggaatgccga caatacgctt gatgtaggtc atctccggct
     1981 gcagaggata attaaaaaca acgacatcgc cccgttcgat tttgcctgta ggaataaata
     2041 tattgtttaa aacgggtacg cgcaggccgt aggaaaattt gccgaccaaa atgaaatcgc
     2101 ccttaatcag gcccgggcgc atcgagctgg acgggatttg gaacggttcg gcaataaacg
     2161 accggatgag gaacaatacc aaaacggtag ggaagaaact gccgaaataa tcgccgaagt
     2221 ggctgctttc cgagatttcg ggatgagtct tcaggcggta tttgtatacc ccccaagccg
     2281 taccgcacaa tacaacgaaa atcaggaaaa cggcggtaaa gctcataaac agggacaaag
     2341 cggcaaacac gccgaccgcc gtcaggatat aggcgtattc cagaccggaa ctccattcac
     2401 cgttttcctg ccgctttttg tcgcttttga aataaaggat gatgccggca agcagcgcgg
     2461 cagccgcgcc cgacattagc attgtgttca ttgttgttcc ttaatgctta aaaacccgcc
     2521 tgtccgtgca accgttttaa ggcggcaaat tgcaaaattt gtttgcgggc gcgtgcccct
     2581 gaaatcaggg cggtttgagg ggtgttcccg acgcgccgcc ctgtgtgcca gagttatttg
     2641 tcgctcacct gcaaaatcgc caagaacgcg ctttgcggaa tttccacatt gcccacttgt
     2701 ttcatacggc gtttacctgc cttttgtttt tcaagcagtt ttttcttacg cgtaatatcg
     2761 ccgccgtaac atttcgccaa aacgttttta cgcagggctt tgacgttttc gcgggcgata
     2821 atctggctgc cgatggcggc ttggacggcg atgtcgaaca tttggcgcgg aatcagctcg
     2881 cgcatttttg atgccagctc gcggcctcgg tgaaccgcgc tttgacggtg cacaatcagg
     2941 cttaatgcat cgactttttc gccgttgacc ataatatcca gcttaatcaa atcagacggt
     3001 tggaactctt tgaaatggta gtccaacgaa gcataaccgc gcgaagtgga tttgagtttg
     3061 tcaaagaagt ccatgacgac ttcgttcatg ggcaaatcgt aagtcagcat cacttggcgg
     3121 cccatgtact gcatattgac ctgaacgccg cgcttttggt tacacaaagt catgacgttg
     3181 ccgacgtatt cctgcggcac aaggatggtc gcggtaataa tcggctcgag tatggtttcg
     3241 atgctgccga tgtcgggcag tttggacggg ttttcaactt cgattttttc gccgcttttc
     3301 aacacgactt cgtaaaccac cgtcggcgcg gtggtaatca aatccatatc gaactcgcgc
     3361 tccaagcgtt cctgcacgat ttccaagtgc aacagaccca agaagccgca acggaagccg
     3421 aagcccaatg cctgggaaac ttcaggctcg aatttcaacg aagcgtcgtt aagctgcaat
     3481 ttttccaaag cgtcgcgcaa ggcttcgtag tcgtggcttt ccacgggata aagtccggcg
     3541 aatacttggc tttgtacttc ttggaagccg ggcagcggct cagtggcagg gttggcaacc
     3601 agtgtaaccg tatcgccgac tttcgcctga cccaattctt ttacgccggt aatcaaaaag
     3661 cccacttcgc cggctttgag ttcttgtttt tgaaccgatt tcggtgtgaa tacgcccagc
     3721 tgctcgacct gcgtttccgc cttggtgctc ataaagcgca ctttgtcttt cagcttgatg
     3781 gtaccgtttt tcactcgaat cagcatgacc acgccgacgt agttgtcaaa ccacgaatcg
     3841 acaatcacgg cttgcagcgg cgcgttttca tcgccggtcg gcgcaggaat tttggcaacg
     3901 atttcttcca aaacgtcttc cacgccgatg ccgcttttgg cggaacactg caccgcgccg
     3961 accgcatcga tgccgatgat gtcttcaatt tcctgctcca cgcgttcggg gtcggcggcg
     4021 ggcaggtcga ttttgttcaa aacaggcaca acctccacgc ccaaatcaat cgcggtgtaa
     4081 cagttcgcca cggtttgcgc ttccacgccc tgcgacgcgt caacgaccaa aagcgcgcct
     4141 tcgcaggcgg acagcgaacg ggaaacttcg taagagaagt cgacgtgtcc cggcgtgtca
     4201 attaggttga gttgatacac ctgcccatcg cgtgctttgt agttgagcgc ggcggtttgc
     4261 gctttaatgg taatgccgcg ctctttttcg atgtccatgg aatcgagcac ctgcgtactc
     4321 atttcgcgca aatccaagcc gccgcaatat tggatgaagc ggtcggcaag cgtcgatttg
     4381 ccgtggtcga tatgggcaat gatggagaaa tttcggatat ttttcattgg attgttttta
     4441 atgctgaacg gtagg
//
LOCUS       DAWKST010000160         4445 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.160, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000160 DAWKST010000000
VERSION     DAWKST010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.160"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(63..1205)
                     /locus_tag="WJL87_001771"
     CDS             complement(63..1205)
                     /locus_tag="WJL87_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ7800176.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQVFDGGVSCRGGL
                     EQVYAANLHSRTAGRILLRLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKIEAKR
                     ANVKSLQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAARRA
                     PGMMRRFGFEKLQNFDKTLWSDLRRRAEAQVKPAFAPISGSDSDRRIIQTALANARSA
                     GVDDIVSFSVADAQSVRPNGVGGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WQVGMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(1254..1676)
                     /locus_tag="WJL87_001772"
     CDS             complement(1254..1676)
                     /locus_tag="WJL87_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800177.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1669..2478)
                     /locus_tag="WJL87_001773"
     CDS             complement(1669..2478)
                     /locus_tag="WJL87_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ7800178.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPGQWTGEPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMMVQTREQWRQAQEKGADVVVWRVCNDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2560..2937)
                     /locus_tag="WJL87_001774"
     CDS             complement(2560..2937)
                     /locus_tag="WJL87_001774"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ7800179.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(3137..3904)
                     /locus_tag="WJL87_001775"
     CDS             complement(3137..3904)
                     /locus_tag="WJL87_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ7800180.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFNMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSIFGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(3914..>4445)
                     /locus_tag="WJL87_001776"
     CDS             complement(3914..>4445)
                     /locus_tag="WJL87_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ7800181.1"
                     /translation="DITFSEDVGECPLHSDSSFSENPESYLVMYVVKSANDGGNSLFL
                     SSSDIVNQLSKTETGKKHLKTLTGNLYPFKAPASFDKKQGVRWGNILSVNTQMIRFRS
                     DCIYKGIEENRNKVSKEMVLALDYLVKIIKNASDIQEFSAQDDGLIIIDNVNGLHAGT
                     DYTDKNRHYIRARITV"
BASE COUNT         1095 a         1115 c         1085 g         1150 t
ORIGIN      
        1 tcggccggat attaaaaaaa gccgtctgaa catttttcag acggcttttt tgtacacttt
       61 cctcaacggt tcgatccctg caccatatca atcaggaaca ggcggcagtc gaggttgccg
      121 ttataaagcg gaattttccg cttgggcgac aggcgcatga atttgggcat ttccctgtcg
      181 ccggtaaaca taccgacctg ccagccggca tagtgttttt tcaaccacgt ccccaactgc
      241 ggatacagtg cctgcaaagc gcggacttcc tcaaggcgca caccgtaggg cggattggac
      301 accataatgc cgcccacgcc gttcggacgg acggattgcg cgtcggcaac gctgaaggaa
      361 acgatgtcgt ccaccccggc actgcgtgcg ttggccaatg ccgtctgaat gatgcggcgg
      421 tcgctgtcgc tgcccgaaat cggtgcaaaa gccggtttaa cctgcgcttc ggcacggcgg
      481 cgcaaatccg accacagcgt tttatcgaaa ttttgcagtt tttcaaaacc gaaacggcgc
      541 atcatacccg gcgcgcggcg ggcggcaatc caagcggctt cgatagcaat cgtgccgctg
      601 ccgcaaaacg ggtcttgaaa cggctgcgtg ccgtcgtagc ctgccgagag cagcaatccg
      661 gcggcaaggt tttcgcgcag cggggcttcg ccggtatcga gacggtagcc gcgtttgaac
      721 agggcttcgc ccgaagtgtc gatgaagatt tcgacattgc gttcgtccaa aaaggcgtgg
      781 atgcgaacat cgggcgcggc tttgtccacg ctcggacgtg cgtcgtaaat gtcgcggaaa
      841 gcgtcgcaga cggcatcttt gacggtcagt ccgacaaatt ggaggctttt gacgttggca
      901 cgctttgcct cgattttgac tttgaacgtc tgctgcaagg taaaccagtt gaaccagttg
      961 atgtttttgg caagtttgta aatgtcgcgc tcggttcggt atgtgccttt ggtcaggcgt
     1021 agcaggatgc ggccggcggt acgcgagtgc aggttggcgg cgtaaacctg ttccaatccg
     1081 ccccggcagg aaacgccgcc gtcaaacact tgtacatcgg tacagccgag gctttcgagt
     1141 tcttgagata aaacggtctc caagccgcgc gggcaggtgg cgaaaagtgt atataaagta
     1201 ttcataacgt gtcctttccg gacggatgtt ccggcaatgg cggattgaaa ggtttattcg
     1261 gcggtaaatg ccgtctgaaa tgtccgactg ccgattgtaa tgtccgccgt aaaatcgcgc
     1321 ctgccttcga cacagatggg caggcggatg cgtactgcct gccaagtccc cgacggttgc
     1381 cgctcgaaca tatagcggtt gaaacccata tccatatttt tcatactgaa gctgatgctg
     1441 acctgttccg tgccggcggg cgcgtgttcg atataaatat caaacggttt tttggttgaa
     1501 acggcggcgg cgcggacgcg gcttccgtcc ggcagcgtgc aaccctcggt caaatcgcat
     1561 tgcgccgcca cagcttgcgg ctgctgcgcc tgccaccatt gcaacaaaac gagcttgacg
     1621 gcggcaaagg caatcagcag cagtgcggca agcagtaatt tacggttttt attcatgatt
     1681 cttccctatt gtttcatccc ttaccaccac gtgcgccccc aatccgagcc atagttttcc
     1741 ataacgtgca accgtctgtc cgtttgctgc aagtacgagc ggcacggata cgccctgccg
     1801 cagggcttct gccgcctctt gtgcctgaac atcattgcac acgcgccaca cgacaacatc
     1861 cgcgcccttt tcctgcgcct gccgccattg ttcgcgggtc tgcaccatca tccacacgct
     1921 gtcggcgtgt tcgggtctgt cctgccattg cgccgcctcc atcaaaacgt tcgcaccgct
     1981 tccctctgcc gaacccaaag gcaggacgcg gtacgcgccc atactgttct ccccgtgcaa
     2041 acccgttttc aggctgccgt acaaacggcg cggaacggac agcgaacgca aaagcgcgcc
     2101 gttggcgggc agcatggggg caacggtaaa atcacccgcc ttctgccaag accattcctg
     2161 cccttcgcgg gattgcggtt cgcccgtcca ttggccgggg ttgacccata ggaatttcag
     2221 gcagacgcgg gcgtgttcgt aggaatggat tttggtcagc caaggcgtgg cggcaaggat
     2281 gcggatgccg agttcttctt caaactcgcg ttgcaaggct tggaagtcgg tttcgcccgc
     2341 ttcgaccttg ccgccggcaa attcccaata tccggcatag ggtttgcctt cggggcgcga
     2401 gctgagcagg tagttgccgt ctgaatcgag caggatgccg gcaacgacgc ggataagggg
     2461 tcgggtgtct tgaatcatgg cgggtacgcg gttggtcggt cggagtgcaa atgagggacg
     2521 agcaatgccg tcaacgggtt tgccgcattt cagacggcat tatttttcgg caacgacaaa
     2581 cgccaatacg gtgtcttctt cgtcgctcat gctgaggctg acgcggctga tgccttgttc
     2641 ctccagccat ttggacaggg cggggccgta gaaaaattcg ggcttgccca atgcgtcatg
     2701 cccgatgccg atgttgcgga aggaaaccgc gccgcgtatg cccgtgccga cggctttggc
     2761 gaaggcttct ttggcggcaa agcgtttggc gaggtagttg acgggtttgc ccgcttgcgg
     2821 aaattcaagc agctcttccg gagtgaggat gcgcccggca aacgcctgtc cgaatttttt
     2881 gtttaagcgg atgatgcgct tgagggaaac aatgtctgtg ccgatgccgt aaatcatatt
     2941 tgcgctcctt cgcccttggt tgcgggtagt gatgaatgtg atgatgaatg ccgtcaattt
     3001 cttggggcgt taaaatggcg tgaatatcca cttttggggt tttgtcggta acaattttca
     3061 ctgtaacctt ttgcatttga aattctaaaa catcgtgcca attatttaat aaggagttaa
     3121 ggcatttaat aatgaattaa agtttgatta aacccgaatg cccgattttc agagccaata
     3181 ccaataaaac aataaaaatt aacattttaa aaaaatttgg gctaatctta gtccttaacc
     3241 gaattccaac atacaatcca ataacagaca atacggacag taaaaatatt aaaccgtatt
     3301 cactcttatt taataaccaa tactggtctc ttagcatata tatttgaaca attttcgcca
     3361 aaagatagca tagattgctt gattttacga tacgattttt attttctgtt tcgctaagca
     3421 aaaatattaa caatatggga gacatggcat tggttgaacc gccgatgatg cctgccaaaa
     3481 acccaaaaag aaccatattc ttattattgg caactacttg aatatttttt gcttttgcac
     3541 atacatttaa aataccattg acagaataat acaatgtaat gattgccatc agtaaaagca
     3601 gccaagacac tggaagtatc aaaagcaact tcaccccaaa aatgctgcca acgacgctgc
     3661 cgatagcaag caatttatag gtttttaaat aataaacaat ctcttgccaa aaaccttttt
     3721 tgttattgct gcatagaacc aacaagctca ttaacaggct tggtaatgcc accaaggcaa
     3781 caaccttaga caatggcatg ttaaaagcca atgcggttgt accgagcatc ggaaatccca
     3841 tgcctgtaat tccgtgcagt attgcggcag caacaaaaac gatagattgc attatttctt
     3901 gcataaccgt cctttataca gtaattcttg ctctaatata atgcctgttt ttatccgtat
     3961 aatcagttcc ggcatgcaag ccattgacat tgtcaataat aatcaaacca tcatcttgtg
     4021 cagaaaattc ttgaatatca ctcgcatttt ttataatctt tacaagataa tcaagtgcaa
     4081 gtaccatttc ctttgatact ttatttctat tttcttcaat acctttatag atacaatcac
     4141 ttctaaatct aatcatttga gtattgaccg ataagatatt accccatctc acaccttgtt
     4201 ttttatcaaa tgatgctggt gctttaaatg gatataaatt gcccgttaat gtttttaagt
     4261 gttttttacc ggtttctgtt ttagataact gattgacaat atctgatgaa cttaaaaata
     4321 gggaattacc tccatcattg gctgatttta ctacatacat aaccaaataa ctttccgggt
     4381 tttcactaaa agatgaatca ctatgaagtg gacattctcc aacatcttcc gagaaagtta
     4441 tatca
//
LOCUS       DAWKST010000161         4369 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.161, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000161 DAWKST010000000
VERSION     DAWKST010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.161"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(35..1414)
                     /gene="norM"
                     /locus_tag="WJL87_001777"
     CDS             complement(35..1414)
                     /gene="norM"
                     /locus_tag="WJL87_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ7800182.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFIDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGTVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVLWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            1749..2900
                     /locus_tag="WJL87_001778"
     CDS             1749..2900
                     /locus_tag="WJL87_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ7800183.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGTVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALDELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPTSERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            3004..4302
                     /locus_tag="WJL87_001779"
     CDS             3004..4302
                     /locus_tag="WJL87_001779"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ7800184.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAILAYYNVQLQHLHNAEPVKAEDVMAVIEKVALRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVETCKPIYETMPGWRESTFGVKSYDA
                     LPANAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
BASE COUNT         1045 a         1196 c         1249 g          879 t
ORIGIN      
        1 tgttaatcca ctatattttt aatcggcgga gttttcagac agccttatgc gatctgacca
       61 tctccctact gcacaattcc aagcaccaca ccaaggcgat ggcggcgatg gtgagcgagg
      121 caatcaatgc cgtccagaag ccgtaaatgc ccatatcgaa acggtaggcg agcaggtagc
      181 ccggcagcag accgcagccc caaaaggcgg cggcgtggat gaacatcggc acctttgtaa
      241 ctttgtagcc gcgcaaggcg taggaggcga tacattgggt gaagtctgcc ggttggaaca
      301 agccggcgaa cagtaagacg gtggcggcga tgcttaaaac cgccggatca ttgttgtaca
      361 tacttaccag cggcgaacgg aataatacca aggaaagcac ggtaatcacg gcgagcatcc
      421 atcctaacac cagtgacacg cccgaaatat aacgcgcccg cgaaaattcg cgccgcccaa
      481 gcgaaaagcc gatgcgcacc gtccccgccg agccgacgct ttgcggaatc atatagagaa
      541 tccccgacaa actgatgccg acctgctgcg ccgccacata atcctcgccg aaaggcgcaa
      601 tcaaaaacac gataaacgaa aacgcgctgg cttccaaaaa ataagacagc ccgatgggtg
      661 cgccgatttt ccaaatctgt ttgaacaccg cccaatccgg tttgccgaat ttcgccgtca
      721 gtccgaatgg gcggaagaaa ttttccttgg cgatataaat ccacaatgcc agcgcgctga
      781 accacaacac tgccatcgtc gccagtccgc agcctgcgcc gcccaaagcg ggcataccga
      841 atttgccgta aacgaaaata tagttcagcg gcacgttcaa cacaaacgcc gcaaagctga
      901 ccaacataat caggcgcggg cggttcaggc tggaggcgta ggcgtgcagt gcgcggtgta
      961 ccattgccgc cggcatcgcc aagctggtga acagcatata ctgcgccatt gtgccttcca
     1021 cataatcgct caaggtcagc cagttgcgga acggcgtaat cgccgcccac atcaataccg
     1081 tgccgaacac gcccaaaaac agcccgaacc aaatcccctg ccgccccgtt tcgcccactt
     1141 cgtcggtttt acccgcgccg taaagctggg caatcatcgg gttcagcgcc gccataatgc
     1201 ccataaaggt aatataaacc gtggcaaacg cgctgctgcc caaagccacc gccgccaagt
     1261 cttccttgcc tgcaccaccc gccatcacag tatcgataaa accgatgccc acctgcgcga
     1321 cctgcgccaa cagcatgggc agggcaaggg cggtcagcag gcggacttct ttcaggaaga
     1381 cggaaaagga aaagcggttg aggtcgagca gcataagtgt tcaatcaaca aaaatgccgt
     1441 ctgaagcaga aaacggcagc aggaagtgat gagaaataat ggtgcacatt atatcgtaaa
     1501 aaaatgctgt gccgtcagac ggcggataca gggtataaaa gtatattcag attgtgtgta
     1561 ttttatggta aagtttggtt ttaacgactt gacggcattg agccgtcgga caggggctgt
     1621 tcggattctg aatcggaagg aagcatcgcc gttttgacag cggcgtgatg cgttgcggca
     1681 aagatgccgt ctgattgcgg atcgggcagc cttttgtatt tacatgataa aatagaaggc
     1741 agattctcat gcagacatgg cagcttccgg aacatatcgc cgacgtactg cccacgaacg
     1801 cgcggcagct tgaaagcgcg agggagcagt tgttggcact gttccgcgta cacggttatg
     1861 aactggtaca gcctccgctg atggagtacg cacattccct gctgacgcat atcgatgcgg
     1921 ggctttccct gaaaaccatt ttggtaacgg acaggctcag cggcaggcag ttgggcatac
     1981 gcgccgacat cacgccgcag gtggcgcgta tcgatgccca tcttttatcc gccaaccaag
     2041 ggattaatcg gttgtgttat gccggtccgg tgttgcacgc gcagcccgac ggtctgctga
     2101 atatgcgcga acccttgcag gcaggggcgg aaatgtacgg ttttgccgac atccgtggcg
     2161 acatcgagct gatagacctg atgctgaaaa gcatgaaaat tgccgatatg ggcaaagtgc
     2221 tgctttcgct ggggcatatc ggcatatttc gcgccttgtc cgatgcggcg catttggatg
     2281 cggggcagtc tgcaacgctg cttgccttga tgcaggataa agataccggg acggtcgaag
     2341 cgcaggtcaa ggcttggaag ctggacggca tgtgggcaaa agcattctcg ctgctgccgc
     2401 gcctgtacgg cgggcgtgaa gtgttgtccg acgcgcgcgg acggttgccg gatttgtcgg
     2461 cggtcggcgg cgcgttagac gaattgcagg cggtgtgcga cgcattcccc gattgtgaaa
     2521 tccatatcga cttgtccgag ctgcgtgtcg acaattacca cacgggcttg ctgtatgccg
     2581 cctatgccgc cgatttccac gacgcggtcg cgcgcggcgg gcgttatgac ggattgggcg
     2641 gatatttcgg cagggcgcgc ccggcaacgg gattcagttt cgacttgcgc agctttatcg
     2701 ggcgtttgcc taccagcgaa cggcagcccg ccgtgttggt cgatgcggaa gatgccgaag
     2761 cggcgcgcga agcagtcgaa gccttgcgtg aacaagggca gtgtgtcgtg attgactacg
     2821 gtatcggaca caatgtttcg gaagagcttg caggccgtct gaaaaagacg gacggcgttt
     2881 ggcaggtcgt gaaacgctaa atacccgttc atggcggatg aaaggcaaat cgtggtgggg
     2941 cgcaaagccg caccggtttg gggatttccg caataatttt taatatcgat aggttatatg
     3001 gctatggcta aaaatgttgt agtaatcggc gcacagtggg gcgacgaggg taaaggtaaa
     3061 atcgttgact ggctggcgga agaagccggc ggcgtggtgc gcttccaagg cggccacaat
     3121 gcgggtcata ctttggtcgt cggcggcaaa aaaaccattt tgcgcctgat tccgagcggc
     3181 atcctgcatg aaagtctgga ctgcttcatc ggttcgggcg ttgtcgtctc ccccgaagcc
     3241 ctgttgggcg aaatcgacga gttaaacgct gcaggcgtga aaaacgtcga aggccgtctg
     3301 aaaatcgcgc cgacctgccc gctgatcctg ccttaccaca tcgcgctcga ccaagcccgc
     3361 gaagcatcgc gcggcaaagg caaaatcggc acgaccggcc gcggcatcgg ccctgcctac
     3421 gaagacaaag tggcacgccg cgccattcgc gccgccgatt tgctgcatcc tgaaaaactg
     3481 cgtgaaaaac tggatgccat ccttgcctat tacaatgtcc aactgcaaca tctgcacaat
     3541 gccgagccgg ttaaagcgga agacgtgatg gcggttatcg aaaaagtcgc gctgcgcatt
     3601 gcgccgatga ttaccgacgt gtcccgcgtg ttgaacgaga aaaacaaaaa cggcgaaaaa
     3661 ctgctgtttg aaggcgcgca aggtgcgttg ttggacatcg actacggcac ttatcccttc
     3721 gttacctcgt ccaactgtct ggcgggagcc gcttccgcag gcgcgggcgt aggccctcaa
     3781 atgctggatt atgttttggg catcgtcaaa gcctatacca cgcgcgtcgg ttcaggcccg
     3841 ttcccgaccg aattgttcga cgaagtaggc gcaggtttgg cggaacgcgg acacgaattc
     3901 ggctcggtaa ccggccgcgc acgccgctgc ggctggtttg atgccgccgc cctgaaacgc
     3961 tccatccaaa tcaacggcat ttccggtatg tgcattacca aactcgatgt aatggacggc
     4021 gttgaaacca tcaatatctg cgtcggctac gaattgcccg acggcggcaa aaccgatatc
     4081 ctgccttgcg gttccgatgc ggtggaaacc tgcaagccga tttacgaaac catgcccggc
     4141 tggcgtgaat ccactttcgg cgtgaaaagc tacgacgcat tacctgccaa tgccaaagca
     4201 tatttgaaac ggattgaaga agtctgcggt gcgccggtcg ccatcgtctc caccggcccc
     4261 gaccgagaag aaacgattgt gctgcatcat ccgttcgcat aaggttttgc agtaaaaatg
     4321 ccgtctgaag cccctccgag ttcagacggc atttttgctg ccgttttat
//
LOCUS       DAWKST010000162         4361 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.162, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000162 DAWKST010000000
VERSION     DAWKST010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.162"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(69..2045)
                     /locus_tag="WJL87_001780"
     CDS             complement(69..2045)
                     /locus_tag="WJL87_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ7800185.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVLTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2194..3033
                     /locus_tag="WJL87_001781"
     CDS             2194..3033
                     /locus_tag="WJL87_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ7800186.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GETVIRDISALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQDAAQN
                     TAS"
     gene            3232..4263
                     /gene="gap"
                     /locus_tag="WJL87_001782"
     CDS             3232..4263
                     /gene="gap"
                     /locus_tag="WJL87_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ7800187.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKIHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAARDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
BASE COUNT         1103 a         1465 c         1015 g          778 t
ORIGIN      
        1 tgcgatttgc gaaagcgtta ttaaaccaaa taaccggaaa tggcgcaagc cttatttcgg
       61 catttcattt atattccctg caaactccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgccttcc aatgtaaacg aggcatccgg gttttcaatc
      181 cggccggtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc gtatgcttct gcagccaaag gaaaggcgtg acggacggtt
      301 tcgccgtttt cgccgaatcc tgcctcatca tcactaccga caaacagtcc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccgata cgacgcgccg
      421 ttgcctaaca tcgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ctatatcggt aaacgttttc acataatcgt cgttggtggc gcgcacatat
      541 acgggcatat tgggatacat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg tcagcaccac cattttcgcc cgtcccagac cggcggcttc caatacttcc
      661 ctgcgtttcg catcgccgaa cgacaccggt tcgcccgcac ttctggcaac ctgcacccgc
      721 gcaatgtcca agtcgagcgc gaaatacgga atatcctctt gggcaaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatggt cggacttgct catggtttct
      841 accagcatac tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caaacgcccg
      901 accagcgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagaaccgcc
      961 gccgtcgccg cctgttccca ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccctgcgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgtt tcattttgaa ggcaatggca aacacaacca gtgccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccag ccgccgatca atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa aaagccgagc aaaatatcgc ggaacgggcg gatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcagcatgc cggcaacgaa tgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgcccgcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaatt ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg acacggcaaa cgcccaattg aacgggacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaac gccgacctgc aaaccaccca gcccgaacac cagccgcctc
     1801 attgccctca gcttgggcag cgaaaactcc aagcctatac tgaacatcag gaacacaatc
     1861 ccgatttcgc ccaaataatc cgtcgcatgg cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaaa acaaccacaa taggggcaag cgaaaattcg
     2041 ttcataggcc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgcattataa caaaacaccg cgcaccatcc gaaacgggcg cggcatacaa ttctgctaaa
     2161 atacgccctt tcgattttga gccgcacacc gacatgaccg ccacagccgc cgccatagac
     2221 cgcctgcttc cccaaaccca atgccgcgaa tgcggctacg acggctgcct gccctatgca
     2281 caggcagtcg caacaggcga agcgtacaac ctctgcgccc cgggcggaga aaccgtcatt
     2341 cgggacattt ccgccctgct cggcaaaccc cttgtcgcac ctgccaaaac ccaagccaaa
     2401 gcactcgccc ggatagacga aaccgcctgt atcggctgca ccgcctgcat ccgcgcctgc
     2461 cctgccgatg ccattatggg cgcgggcaaa cttatgcaca ccgtcatcgc cgacgaatgc
     2521 accggctgcg gactctgcgt cgccccctgc cccgtcgact gcatccatat gcagcccgtt
     2581 gccgacaccg tcctgccccg cgcgcgtcgc ttcagcctgt ccgccgacag ccgttttgcc
     2641 gccgccgaac acgcgcgcgc gcgctacctc aaacgcaacg aacgcaaaca gcgcgaagcc
     2701 gacgaacgca aggccatgct tgccgaacgc gaagccgccg tccgcaacgc gcgtccgcaa
     2761 acacccgaca caccgaaaaa accggcgttt aaccctgccg acctcatcgc caaagccatg
     2821 gcaaaagcgc aaacccaaca agaccgcctc gccgccgccg acaaccgcaa agactatcag
     2881 gcgaaacaga tagccgaagc ccgcgaacgc gccgagttgc gccgcgccca acgcgatatg
     2941 aaatacggca gcgacagcga aaaagccgcc gccctcgaat acctcaaaca atacaaagcc
     3001 aagcaggatg ccgcacaaaa caccgcctcc tgacccttcc ctgatatgcc gtctgaagcc
     3061 gcttcagacg gcattttatc aagctctccg tccgccgcac ccgtgccgtc cgcatcttac
     3121 cgcccaccct tccggccgcg ccgttttcaa taaaatatta attacacgcc actacaaatt
     3181 tgctataatc cgccccgaaa atctacccaa ccctcaacaa aggaacgaac catgggcatc
     3241 aaagtcgcca tcaacggcta cggacgcatc ggccgccagg ttttgcgcgc catctacgat
     3301 tatcagattc aagaccaact ccaaatcgtc gccgtcaacg ccagcggtag ccttgaaacc
     3361 aacgcccacc tcaccaaatt cgacaccgtg cacggacgct ttgaagccga cgtatcccac
     3421 gacggcggca acctcatcgt caacggcgac aaaatcccct tcttctcgac ccgcaaccct
     3481 gccgaactgc cgtggaaaga actcggtgtc gatttggtca tggaatgcac cggcgcgttt
     3541 accagcaaag aaaaagccaa aatccacctc gaaagcggcg cgaaaaaagt cctcatttcc
     3601 gcaccgggcg gcgacgatgt cgatgcaacc gtcgtgtacg gcgtgaacga cagcgtcctg
     3661 accgccgaca tgaccgtcgt ttccaacgct tcctgcacca ccaactgcct ctcgccggtt
     3721 gccaaagtgt tgagcgaaag cgtcggcatc gtcaaaggcg cgatgaccac catccacgcg
     3781 ctgaccaacg accaaaccgt taccgacgtg cgccacaaag acctgcgccg cgcccgcagc
     3841 ggcgtggaaa acatgattcc gaccaaaacc ggcgcggcaa aagccgtcgg tttggtactg
     3901 cccgaattga aaggcaggct cgacgggctt gccatccgcg tgccgaccgt caacgtatca
     3961 ttggtagatt tgagcttcca agccgcgcgc gacacgacag tcgaagaaat caacgcactg
     4021 atgaaagccg cctcggaagc aggcgcgctc aaaggcgttt taggctacaa caccctgccc
     4081 ttggtttcca tggacttcaa ccacactacc gaagccagcc acttcgacgc aacactgacc
     4141 aaagtcgttg acggcaacat gattaaagtc ttcgcatggt atgacaacga atggggcttc
     4201 agctgccaaa tgctgaacac cgcacgccgt atgttcggac ttgaagtgcg cccgctcaaa
     4261 taagcaataa accgtcaaac aaaatgccgt ctgaaacccg atgttttcaa gttccagacg
     4321 gcatttttca ccgtgatttt acccggctgt cgtcatttcc a
//
LOCUS       DAWKST010000163         4260 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.163, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000163 DAWKST010000000
VERSION     DAWKST010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.163"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            537..2264
                     /gene="ilvB"
                     /locus_tag="WJL87_001783"
     CDS             537..2264
                     /gene="ilvB"
                     /locus_tag="WJL87_001783"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ7800188.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKVDIPIVGDVKNVLSEMVALW
                     QKQEFMPSEDALGKWWKTIEEWRSQDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADEKA"
     gene            2275..2766
                     /gene="ilvN"
                     /locus_tag="WJL87_001784"
     CDS             2275..2766
                     /gene="ilvN"
                     /locus_tag="WJL87_001784"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ7800189.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            2833..3126
                     /locus_tag="WJL87_001785"
     CDS             2833..3126
                     /locus_tag="WJL87_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ7800190.1"
                     /translation="MSNIKIVALVTVKPEYTETLVAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAA"
     gene            3206..4219
                     /gene="ilvC"
                     /locus_tag="WJL87_001786"
     CDS             3206..4219
                     /gene="ilvC"
                     /locus_tag="WJL87_001786"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ7800191.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVTANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
BASE COUNT         1105 a         1014 c         1077 g         1064 t
ORIGIN      
        1 tcaatatatt aagtcgttgt tagccctctt ggttctgtca atgccgtcca atacagtccg
       61 gtgcaatgag ccattttcag gaagtatggt tgggaatatt tttgatatat caaaaataaa
      121 atcaagttca atcaatatcc cgagtcttta ttgggatgaa accctgtaaa ttttacaatt
      181 cttgatatcg aagaagggtt tttcacatct gcccctccta cacaaactgt caacaatttc
      241 catctttcaa aaaaggtact cctgatttta ttcttttaaa atcaatcaat aatttctctg
      301 ctttcaaaaa atattaaaag atgaaagatt tttcttgatt gaactgtcgg tttgcgttat
      361 cgtttctact ctttttcaca aactctgtgt tcctttccaa ttgattggat aatccgtctg
      421 ttgccgtgtt cccccaatgc gcgtacccta aatcgctgct tggtggaatt gcattacagg
      481 tgctgtggca aagcggtttg tccctattgg tttgaaaccg tataaaagag gtcgttatgc
      541 agttatcagg cgcgcaaatc atagtgcaga gtctcaaagc cgaaggtgtc gagtacgttt
      601 tcggttatcc cggcggtgcg gttatcgaaa tctacgatgc ccttttccaa ctcaataaat
      661 tcaagcacat tctgacccgt cacgagcagg cggcagtaca cgcggcagat gcgtatgcgc
      721 gcgtcagcgg taaggtgggc gtggcattgg ttacatccgg cccgggcgtt accaatgcac
      781 tgaccggtat tgctactgcc tatacggatt cgattccgat ggtggtcatc agcgggcagg
      841 taggcaattc cctgattggt acggatgcgt tccaagaagt tgatacggtg ggtattaccc
      901 gtccgtgcgt caaacacaat ttcctggtta cggacatcaa tgagttggcg gaaaccatta
      961 aaaaggcgtt ccaaattgcc gcaagcggcc gaccggggcc cgtggtggtt gatgtcccga
     1021 aagatgttac gcaggcgatg gcgaaattca gctatcctca ggaagacatt tttatccgtt
     1081 cgtatcaacc ggttgttcaa gggcatatcg gtcagattaa aaaggccgtg cagatgttgg
     1141 catctgccaa acgtccggtc gtctattttg gcggcggcgt ggtgttgggt aatgcttctg
     1201 aagagctgac ccgatttgtc cgaatgacgg gtgctccgtg cacgggtacg ctgatgggct
     1261 tgggcgctta tccttccggc gaccgccaat tcctcggtat gctcggtatg cacggtactt
     1321 acgaggcaaa ccttgccatg cagaatgcgg atgtcgttct tgccgtaggt gcgcgttttg
     1381 acgaccgtgt ggtttccgta ccgtccaaat ttttcgagaa ggcgaagaag gttatccata
     1441 tcgatgtcga tccttccagc atcgccaaac gcgtgaaggt ggacattccg attgtcggcg
     1501 acgtgaaaaa tgttttgtct gagatggttg cgctgtggca aaaacaagag ttcatgccgt
     1561 ctgaagatgc tttgggcaaa tggtggaaaa ccatagagga atggcgttcc caagattgct
     1621 tgtggtttga caacggcagc gaaattatca agccacaata tgtgattcag aagcttgccg
     1681 agattaccgg caattcggca atcatcacat cggatgtagg gcagcatcaa atgtttgcgg
     1741 ctcaatatta tcccttcgaa cgtccgcgcc aatggctcaa ttccggcggt ttgggtacga
     1801 tgggcgtggg tctgccttat gcgattggtg caaaacttgc cgccccggat caagacgtat
     1861 tctgtattac cggcgacggc tcgattcaga tgaacatcca agagttgtcc acctgtttcc
     1921 aatatcggat tccggttaac gtcattacgc tgaacaacgg ttatctcggt atggtacgcc
     1981 agtggcagga aatatattac ggcggccgag agtcggaaac ttatttcgat tctttgcccg
     2041 atttcgtcaa acttgccgag gcatacggcc atatcggtat ccgcgtggac aagaagtctg
     2101 atgtggaagg tgcgttgttg gaagcattga accaaaaaga caggctggtg tttatcgact
     2161 tcctgaccga ccagaaacag aatgtgatgc ccatggtcgg caacggcaaa ggtttggacg
     2221 aaatggtact tccgccgcat atgcgtgcgg acgaaaaggc gtaaggagag gcaaatgcga
     2281 catatcttat ctgttctgat tgaaaacgaa tcaggtgcga tgagccgcgt ggtcggtttg
     2341 ttctctgcac gcgattacaa tatcgattct ttggcggttg cgccgaccga agacaaaacc
     2401 ctgtcccgta tgaccatcgt tacccacggc gacgagcagg ttatcgaaca aatcaccaag
     2461 caactcaata aattgattga agtgattaaa gtggtcgatt tgaacgaaag ccgttttgtc
     2521 gaacgcgaac tgatgttggt aaaagtccgt gccgccggca aagaccgcga cgaatttttg
     2581 cgtttgaccg aaatctaccg gggcagcatc atcgacgtaa ccgaccgcag ctatacgatt
     2641 gaaattaccg gctcgaccga caagctggat tcctttttgg aaacggtcgg acgcgcccaa
     2701 attttggaaa ccgtacgtac aggcgcggcc ggcatcggtc gcggtgagcg tattttgaaa
     2761 atttaacgcc gtaacctttc agacggcacg gtatttgaat gccgtctgaa aaacgaacgg
     2821 caggagagat ttatgtcgaa cattaaaatt gtcgcactgg ttaccgtcaa accggaatac
     2881 acagaaacac tggtagcaca gtttaaagaa ctggtcaaag ccagccgtgc agaagagggc
     2941 aacatcagct acgatctcca tcaggaaatc ggtaaaccga accgttttgt tttcgtggaa
     3001 aattggaaat cccaagcagc tattgacgaa cataacgcca gcgcgcattt ccaagccttc
     3061 gtccagtccg tcgacggcaa aaccgaagcg ttggaaatcg tattgatgaa tgaagttgcc
     3121 gcttaagcgg cacactctgt ttaacccgtc tgaagccgct caacatttta aggcttcgac
     3181 aaccatttac cttaaaggaa atcaaatgca agtctattac gataaagatg ccgatctgtc
     3241 cctaatcaaa ggcaaaaccg ttgccatcat cggttacggt tcgcaaggtc atgcccatgc
     3301 tgccaacctg aaagattcgg gtgtaaacgt ggtgatcggt ctgcgccaag gttcttcttg
     3361 gaaaaaagcc gaagcagccg gccatgtcgt caaaaccgtt gctgaagcga ccaaagaagc
     3421 cgatgtcgtt atgctgctgc tgcctgacga aaccatgcct gccgtctatc acgccgaagt
     3481 tacagccaat ttgaaagaag gcgcaacgct ggcatttgca cacggcttca acgtgcacta
     3541 caaccaaatc gttccgcgtg ccgacttgga cgtgattatg gttgccccca aaggtccggg
     3601 tcataccgta cgcagtgaat acaaacgcgg cggcggcgtg ccttctctga ttgccgttta
     3661 ccaagacaat tccggcaaag ccaaagacat cgccctgtct tatgcggctg ccaacggcgg
     3721 caccaaaggc ggtgtgattg aaaccacttt ccgcgaagaa accgaaaccg atctgtttgg
     3781 cgaacaagcc gtattgtgcg gcggcgtggt cgagttgatc aaggcgggtt ttgaaaccct
     3841 gaccgaagcc ggttacgcgc ctgaaatggc ttacttcgaa tgtctgcacg aaatgaaact
     3901 gattgttgac ctgattttcg aaggcggtat tgccaatatg aactactcca tttccaacaa
     3961 tgcggagtac ggcgaatacg ttaccggccc tgaagtggtc aatgcttcca gcaaagaagc
     4021 catgcgcaat gccctgaaac gcattcaaac cggcgaatac gcaaaaatgt ttatccaaga
     4081 gggtaatgtc aactacgcgt ctatgactgc ccgccgccgt ctgaatgccg accaccaagt
     4141 tgaaaaagtc ggcgcacaac tgcgtgccat gatgccttgg attactgcca acaaattggt
     4201 tgaccaagac aaaaactgat tgttttcaaa cgggactgcc tacacatcgt gtaggcagtt
//
LOCUS       DAWKST010000164         4210 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.164, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000164 DAWKST010000000
VERSION     DAWKST010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.164"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(83..1129)
                     /gene="recA"
                     /locus_tag="WJL87_001787"
     CDS             complement(83..1129)
                     /gene="recA"
                     /locus_tag="WJL87_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ7800192.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEIADEIDAKIRALNGIEMHIT
                     EGTQDETDGERPEE"
     gene            1383..2147
                     /locus_tag="WJL87_001788"
     CDS             1383..2147
                     /locus_tag="WJL87_001788"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ7800193.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLEQIKNMPFD
                     IAEFRADFLECAGSIGEILHHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFKLL
                     DALIESRLPDIIDIELFSGETAVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLKAKELAAKPIVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRATLDCLENGAD"
     gene            2167..4182
                     /gene="rep"
                     /locus_tag="WJL87_001789"
     CDS             2167..4182
                     /gene="rep"
                     /locus_tag="WJL87_001789"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ7800194.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKYLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLHKSQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNIWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSKLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVSDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDSMKKGNPSD"
BASE COUNT         1003 a         1148 c         1150 g          909 t
ORIGIN      
        1 aaactcaaat caagccgttc ggaggctgct caaaaaaaac ggtacttcgc aacagaagta
       61 ccgtttatcg ggatttcagg ttttattctt cggggcgttc gccgtcggtt tcgtcctgcg
      121 tcccttcggt gatgtgcatt tctatgccgt tgagggcgcg gatttttgcg tcgatttcgt
      181 cggcaatttc gggattttcc ttcagccaga cgcggacgtt gtctttgccc tgaccgattt
      241 tcgcgccgtt gtagctgtac cacgcgccgg atttgttgat gatgtcgttt ttcacgccga
      301 tgtcgatcaa ttcgccttcc caactgatgc cttctccgta gaggatgtca aactctgcct
      361 gacggaacgg cggcgcgact ttgtttttga tgactttgac gcgggtttcg ttgcccaata
      421 cttcttcacc ttttttgatg gatccggtgc ggcggatgtc gaggcggacg gaagaataga
      481 atttcagcgc gttgccgccg gtcgtggtct cggggctgcc gaacattacg ccgatcttca
      541 tccggatttg gttgatgaac acaaccagcg tgttggtttt tttgatgtgt ccggtcagtt
      601 tgcgcaaagc ctggctcatc aggcgcgcct gcagtccgac atggctgtcc cccatatcgc
      661 cttcgatttc ggctttgggg acgagtgcgg ctacggaatc gacgactacc atatctatgc
      721 cgcccgaacg gacgagtgtg tcgcagattt ccaaagcctg ttcgccggta tcgggctggg
      781 acaggtaaag ctcttcgact tttacgccga gtttgcgggc gtaaacggga tcgaaggcgt
      841 gttcggcatc gacaaaggcg cacacgccgc cgtttttctg gcattgggcg acggcttcga
      901 ggcagagggt ggttttgccg gaggattctg ggccgaagat ttcgacgatg cgcccgcgcg
      961 gcagaccgcc gactccgagg gcgaggtcta atccgagcga tccggtggaa atgacttcga
     1021 ggttttcttc ctgctggctg ccgtccattt tcatgatggc gcctttgccg aaactttttt
     1081 cgatttgcgc cagtgcggcg gcaagtgctt tgcttttgtc gtctgacatt ggggttactc
     1141 cggaacaaat gcggtatgtg ggatgcggcg caacacgggc tgcggcgcgg gatgtgtatc
     1201 gttttcccga tgtgcgggct atcggtaatg ctgcttcacg aggttgccat tatcgcatat
     1261 ttccttgctt gccgatatgc gccaggacgc ggcggcttgt gccggaatgg aatctggatg
     1321 ccgtctaaaa ggcggccggc tttgttataa tggcggctgt tttttctgtg tgtgcctgtt
     1381 ttatgtgttc ctgccttgtt gtcaaaaata ccgttatcgg aagcggacgc accaaaatcg
     1441 ccgtgccgct tgtcgcccgc gatgccgccg aactttccgc cgtacttgag caaatcaaaa
     1501 atatgccctt cgatattgcg gagttccgcg ccgacttttt ggaatgcgcg ggcagtatcg
     1561 gcgaaatatt gcaccacacg cagaccgtcc gcgacgcgct gcccgacaag ccgctgctgt
     1621 ttacgttcag acggcatggc gaaggcggct cgttcccgtg ttcggacgat tattatttta
     1681 aactgctcga cgcgctgatc gaaagtcgcc tgcccgacat tatcgatatc gagctgtttt
     1741 ccggcgaaac cgccgtccgg cgcgctgtgg cgaatgctca aaaaaacggc atcgccgccc
     1801 tgctctgcaa tcatgagttt caccgcacgc cgccgcaaga agaaatcgta tgccgtctga
     1861 aacagatgga ggactgcggc gcggacatct gcaaaattgc ggtgatgccg caaagcgcgg
     1921 aagatgtgct gactttgctt tccgccacgc tcaaagcgaa agagcttgcc gccaaaccga
     1981 ttgttacgat gtcgatgggg cagacggggg cggtcagccg gcttgccgga caggtgttcg
     2041 gctcaagcat cacgttcggt tcgggaacgc aaaactccgc gccggggcaa atcggcgtat
     2101 ccgccctccg tgcgacactc gactgcctcg aaaacggcgc agactgattt cagacagcat
     2161 caaaacatga tgaaactcaa tccccaacag ctcgaagccg tccgctacct cggcggccca
     2221 ctgctcgtcc ttgccggtgc aggcagcggc aaaaccggcg tgattactca aaaaatcaag
     2281 tatttgattg tcaatgtcgg ctatctgccg cataccgttg ccgcgattac ctttaccaat
     2341 aaagccgccg cggaaatgca ggagcgcgtc gccaaaatgc tgcacaaatc gcaaacgcgc
     2401 gggctgacga tttgcacgtt ccactctttg ggcatgaaga ttctgcgcga agaggcgaac
     2461 catatcggtt acaaaaaaaa cttctccatt ctcgattcta ccgacagcgc gaaaatcatc
     2521 ggcgaactct taggcggtac gggcaaagaa gccgtattca aggcgcagca ccagatttcc
     2581 ttgtggaaaa acgatttaaa aacgcctgaa gatgtcgttc agacggcatc gaacatttgg
     2641 gaacaacaaa ccgcacgcgt gtatgcgagc tatcaggaaa ccttacaaag ctatcaggca
     2701 gtggacttcg acgacttgat ccgcctgcct gccgtgctgt tgcagcaaaa cagcgaagtg
     2761 cgcaacaaat ggcagcggcg gctgcgttat ctgctggttg acgaatgcca agatacgaat
     2821 acctgccaat ttacgttgat gaaacttctg accggcgcag aaggtatgtt taccgccgtc
     2881 ggcgacgacg accagtccat ctacgcatgg cgcggcgcga acatggaaaa cctgcgcaaa
     2941 atgcaggaaa actatccgca gatgaaggtc atcaaactgg agcaaaacta ccgctccacc
     3001 gcgcggattc tcaaaatcgc caacaaagtc atcgagaaca accccaagct gttcaccaaa
     3061 aaactttggt cgaaattggg cgaaggcgag cccgtcaaag tcgttgcctg ccaaaacgag
     3121 cagcacgaag ccgactgggt cgtcagccaa atcgtcaaac aaaaactcat cggcggcgac
     3181 aaaacccaat atgccgattt cgccgtgttg taccgcggaa agcatcaggc gaggattttc
     3241 gaggaagcat tgcgcggcgc gcgcatcccc taccgtctct ccggcggaca aagctttttc
     3301 gacaaagccg aaatcaaaga cgtgttgtct tatgtgcggc tgcttgccaa ccccaatgac
     3361 gatcccgcct ttctgcgtgc cgttaccacg cccaaacgcg gcatcggcga tgtcacgctg
     3421 ggcaagctca acacttacgc gcacgaacac gaatgcagcc tgtatgaagc cgcacaaaac
     3481 gaagaagccc ttgccacgct gaacaatacc aaccgccaac acctgcaagc ctttatggat
     3541 atgttcggca gctaccgcgc caaagcggaa accggcgaag caggcgagtt catcaacagc
     3601 ctgctcgaag aaatcgacta tgaaaaccat ttgatgcaaa acgaagaagg caaggccggc
     3661 gaaatcaaat ggcgcaatgt cagcgatttg gtatcatggt ttgcgcgaaa aggcggggaa
     3721 gacggcaaaa acatcatcga actcgcccaa accgtcgcct tgatgacgct tttggaagga
     3781 aaagacgaag aagaaaccga tgccgtctcg ctatccacgc tacacgccgc caaaggtttg
     3841 gagtatccgt atgttttcct tgtcggttgc gaagaaggcg ttttgccgca caacgacagt
     3901 atcgaagagg gcaacgtcga agaagaacgc cgcctgatgt acgtcggcat cacccgcgcc
     3961 aaacgccaac tcacgctgac ccactgcgtc aaacgcaaaa aacaaggcac atggcagttc
     4021 cccgaaccca gccgattcat agacgaaatg ccgcaggaag atttgaaaat cctggggcgc
     4081 aaaggcggcg agccgattgt cagcaaggaa gaaggcagac gcaaccttgc cgatataatc
     4141 ggaaggctcg acagcatgaa aaaaggcaat ccgtcggatt aaaccaagat agggaaacga
     4201 agccggagac
//
LOCUS       DAWKST010000165         4192 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.165, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000165 DAWKST010000000
VERSION     DAWKST010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.165"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(79..315)
                     /locus_tag="WJL87_001790"
                     /pseudo
     CDS             complement(79..315)
                     /locus_tag="WJL87_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
     gene            511..969
                     /locus_tag="WJL87_001791"
                     /pseudo
     CDS             511..969
                     /locus_tag="WJL87_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            869..1237
                     /locus_tag="WJL87_001792"
     CDS             869..1237
                     /locus_tag="WJL87_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ7800195.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDDLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLIGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            1306..2844
                     /gene="lnt"
                     /locus_tag="WJL87_001793"
     CDS             1306..2844
                     /gene="lnt"
                     /locus_tag="WJL87_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ7800196.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            3108..3971
                     /gene="rpoH"
                     /locus_tag="WJL87_001794"
     CDS             3108..3971
                     /gene="rpoH"
                     /locus_tag="WJL87_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ7800197.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
BASE COUNT         1020 a         1295 c          967 g          910 t
ORIGIN      
        1 cgaccttgcc gtcgggcagg atgtaccacg ccaaatcccc gcctgaagca cggtgtcgga
       61 ctgcatttcg attcggctat acgccttttc acgcaggctg taattgaatt tccacaaacc
      121 gccgaacact gccggcggac gcgcgaccag catcaggaac gccgcaatcg ccaaggcttc
      181 cgccgccccg tccaacacgc cggcgggaga aaccagcagc aaaggcattc ccaaacgtgc
      241 gggtaaagtg gtcgaaacca cgctcagaaa caacagtgcg ccacccggca ggaacagact
      301 gcccatccaa tccacgctaa cgattctccc ttaaaattca ttaaattaaa atcgaaagac
      361 tgtaacacgg cattttcttt tcacgaaact ttacttcacg tccgccacag cgttcagacg
      421 gcattttcct gtttcatgcc gcctgaaacc gcccaaatcc gcgttctcct atataatgtc
      481 ggactttgcg ccgcatttca ggattcttta atggcatttg cctcactatt taccctgccg
      541 gacgacatca ccgccgtttt ggacgatgtt gccctgatga ccaaagccgc cgcaaagaaa
      601 accgccggcg tggtcggcga cgatttggca cccaatgcca accgtgttac gggcgtatcg
      661 gcagaacgcg agttgccgat tgtttggtcg atggcgaaag gttctttttc caacaagccg
      721 gttttggtgc ctgcggccct gctgctttcc gccttcctgc cgcaactcat tacgccgctt
      781 ttgacggcag gcggcattta tctgtgcttt gaaggcgtag aaaagctgct gcacaagttt
      841 ctgcaccgac atgacgcgca tgctcgtaat ggcggcaatc ggcatcggca tgactgtttt
      901 ggtgtacgga atcgttgccg tcatcgtcaa actcgacgac ttaggcatgc tgctgatgcg
      961 ccgcccacaa accttttcgc gcagtttggg gcaaatgctg actgccttta tgccttgctt
     1021 tatgcgcggt ttgagcgtgg tcggcacgct tgccatgttt cttatcggcg gcgtactggt
     1081 cgcccacaac ctcggccttc tgcacgattt cctgcacgcg caacactggg acgctggctg
     1141 ggcggagtac ttcgccaacc tcgttgtcgg gctgctttcc ggttcgattg cctgcgcccc
     1201 cgccttgccg ctgatgaatc gtttcggcag gcattgattt ctttttcaaa taccgatgcc
     1261 gtttgaaaga tgttcagacg gtatcttccg aacagacaga tgaatatggt ttccaaactg
     1321 gacaaatact ggcagcaccc cgccctctac tggcctttgc tcatcctttt tgccgccgcc
     1381 acccccttta ccttcgcacc ctactaccac ttttggctga tgcccttgat tttcggtgcc
     1441 ttcgtccgcc tcatcgaact gcgtccgcgt tttgctgtct cttccgccta cctgttcggc
     1501 ctgaccgcat acacgacaca gttctactgg atacacaccg ccctgcacga cgtttccggc
     1561 ctgcccgacc tctatgccgt accgctgacc ttcctactcc ccgcctacct tgccctttat
     1621 ccggcactgt gtttctggct gtggaaaaaa tttaccctgc ctcggggcat aaaaatcggt
     1681 ttggtactgc ccatcctgtg gacactgacc gagtttgccc gcgaacgttt cctgaccgga
     1741 ttcggctggg gcgcaatcgg ctactcccaa atcaccccgg acagcccgct cgccggcttt
     1801 gccccattgg gcggcatcca catggttaca ctggcaaccg cctttctcgg tgtctggctg
     1861 gttttggcga gtgataacac cgcacgttcg ggcaaacgcc tgcttccaat tatcctgatt
     1921 gccgccctgc ttgccgcagg ctacaccgcc cgacaaaccg acttcacccg ccccgacggc
     1981 agccgcagca ccgtcgccct gcttcaaggc aacatcgacc aaaccctcaa atggcgtgaa
     2041 gaccaagtca tcccgaccat acagaaatat tacgaacagg tcggcaaaac cactgccgac
     2101 atcgtcatcc tgcccgaaac cgccatcccc gttatgcgcc aaaacctgcc ggaaaacata
     2161 ctggcaaaat ttgccgaaca ggcgcaaaac aacggcagcg cgctcgccgt cggcatcagc
     2221 caatacactt cggacggcaa cggttacgaa aacgccgtca tcaacctgac cggttatcag
     2281 gaaaacaatc aggacggcat cccctactac gccaaaaacc acctcgtccc cttcggcgaa
     2341 tacaaaccgc tgcccttcct gaccacgccg ctttacaaaa tgatgaatat gcccctttcc
     2401 gacttccgca aaggcggcgg taagcaatcc gccctgctga tgaaaaacca aaaaatcgcc
     2461 ttcaacatct gttacgaaga cggattcggc gacgaactga ttgccgccgc caaagatgcc
     2521 acactgcttg ccaatgccag caatatggcg tggtacggaa aatccaacgc catgtaccag
     2581 cacctccaac aatcgcaggc gcgggctatg gaactcggac gctatatggt ccgtgccacc
     2641 aacaccggcg caaccgccat catctccccc aaaggcaaca tcatcgccca agcccaaccc
     2701 gatacggaaa ccgtattgga aggacacatc aaaggctatg tcggcgaaac gccctatatg
     2761 aaaaccggca gttcatggtg gttgatgggc atattgaccc tagccgcact gattcttttc
     2821 atcttccgaa acaaagaaca ctgatacctg cacagcccgt ccggacgggc tgtctttttt
     2881 caaaacggct atttacatac aattacgtat gtaatataat tctgaaatcg aattgagcca
     2941 tcaggtttac aaaatcaatc aaacctgcct tacaaaattt tcctgtcttt caaaccttag
     3001 cgcatatctc ctgccaaaca tacaaaatac aagccaagca gtgatatact gcatcccaac
     3061 acagcaaacc gccgatacgc agtttcagcc gaaaggaaat gccccaaatg aataacgctt
     3121 tcgcattacc cgccatccaa agcggcaacg gcagcctcga acaatacatc cacaccgtca
     3181 acagcatccc gatgctgtcc caggaagaag aaacccgcct cgccgaacgc cggataaagg
     3241 gcgacctcaa cgccgccaaa caactcatcc tgtcccacct gcgcgtcgtc gtttccatcg
     3301 cgcgcggcta tgacggctac gggctgaatc aggcagacct gattcaggaa ggcaatatcg
     3361 gactgatgaa ggcggtcaaa cgctacgagc ccggtagagg cgcgcgtctg ttttcatttg
     3421 ccgtacactg gattaaagcc gaaatccacg agtttatttt gagaaactgg cgtttggttc
     3481 gcgttgccac caccaaaccg caacgcaagc tgtttttcaa tttacgcagt atgcgtaaaa
     3541 acctcaacgc cctgtctccc aaagaggcac aagacatcgc cgacgatttg ggtgtcaaac
     3601 tttccgaagt tctggaaatg gaacagcgca tgacgggaca cgacatcgcc atcatggcag
     3661 acaacagcga cgacgaggac agtttcgccc ccatcgactg gcttgccgac cacgattccg
     3721 aaccgagccg gcaactatcg aaacaggcgc attacgccct gcaaacagaa ggtttacaaa
     3781 acgctttggc gcaactggat gacaggagcc gccgtattgt ggaaagccgc tggcttcaag
     3841 acgacggcgg gctgacgctg caccagcttg ctgcagaata cggcgtctct gccgagcgca
     3901 tccgccagat tgaagcaaaa gccatgcaga aactgcgtgg tttcctgacc gaagaagccg
     3961 aagcggtttg aaatatacgc aataaaatac ccgacaaatt gttttgtcgg gtattttatt
     4021 tggtaaacat caatgcggtt tgtgcgtcgt gctgtacacg attttatccg tccacgccgt
     4081 tgcaattgcc gaaatcaaaa agcagacatg caccagacac tgccgcatca gctgcttttc
     4141 cgacagattg gcggaattga tatagtggat taacaaaaat caggacaagg cg
//
LOCUS       DAWKST010000166         4183 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.166, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000166 DAWKST010000000
VERSION     DAWKST010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.166"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            230..1150
                     /gene="ribF"
                     /locus_tag="WJL87_001795"
     CDS             230..1150
                     /gene="ribF"
                     /locus_tag="WJL87_001795"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ7800198.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADT
                     RGLPVVTVVFEPQPKEFFALRTGRMPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSDCFQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRQIEADMEAAKCW"
     gene            1291..4080
                     /gene="ileS"
                     /locus_tag="WJL87_001796"
     CDS             1291..4080
                     /gene="ileS"
                     /locus_tag="WJL87_001796"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ7800199.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQVACQKKDFIRLGVLGDWDKPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVGYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGDHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYISETPRVAGMSVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKGELLSAEDCEHYDKLSDTMDVWFDSGSTHYSVVKQREELDWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDQNGRKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYAVVLARQLQERLAGDYYPRYTFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGREGETRHYA"
BASE COUNT         1021 a         1170 c         1112 g          880 t
ORIGIN      
        1 tttacctggt taaaaaagaa cgattttcac tgatgttgca tcaggtttgg ggcgatgttt
       61 caacacatag caccgcgcct gctgcgcgct ttatgcgttt ggcgcgttcg gctgcgggaa
      121 atttgcctac ttttcccgcg tcgggcgggc gtaacgggcg gcacactgtc tataaaccgc
      181 aataccgttt acaatgaccg cctgtttcac cacatacccg aacataacaa tgagaatcag
      241 gctggggcgg cacaacgcgc ccgactttcc gcacggggcc gccgtaacca taggcaattt
      301 tgacggcgta cacctcggac acaaacacat cctccaaaaa ctccgcctcg aagccgatac
      361 gcgcggatta cccgtcgtga ccgtcgtttt cgaaccccaa cccaaagaat ttttcgcact
      421 ccgcaccggc aggatgccac cgtgtcggat cagccccctg cgcaccaagc tcgaattatt
      481 ggaaggcaca ggctgtgtcg atgccgtctg ggttttgcgt ttcgatcaaa atttttccga
      541 aatatccgcg caagggttta tcgaccgcct gctgcgtcaa accttgaata cgcgttattt
      601 gctcgtcggc gacgatttcc gtttcggtgc ggggcgggaa ggctgttttg aacttttggc
      661 acaacagccc gatatgcaga ccgagcgtac gccttccgtc atcgtcgaag acatccgcac
      721 cagcagtacc gccgtgcgac aagccctttc agacggcaac cttgcctatg cgaaaaaact
      781 tttgggacac gactacgtct tgagcggcag ggtggtgcac ggcagaaaac tcggacgcac
      841 cttaaacgcc ccgactgcca acatccgcct gccccgccac cgttatgcac tcggcggcgt
      901 gttcgtcgtc gaagcggacg gcgcgttcgg cacgcggcgc ggcgtggcga gcttcggctt
      961 caatcccacc gttgatagcg actgttttca aaagctggaa gtccacctgt tcgactttca
     1021 aggcgatttg tacggacaac ggttgaacgt ccgcttcctg cacaaactgc gcgatgagga
     1081 aaagtttgac ggtatggaag aactgaaaag gcagattgaa gccgatatgg aagccgcaaa
     1141 gtgttggtag aaaaacctta tacaaaccat ccgattgggc tacaatcaac cttttaactg
     1201 ttcagacggc ataggggttt cccgttgtga aatactgttt gaggcgcaat gccgtctgaa
     1261 ccgaaatatt gtaacaatag agattaaaaa atgaccgatt acagcaaaac cgtaaacctg
     1321 ctcgaaagcc cgtttccgat gcgcggcaat cttgccaagc gcgagcctgc atggctgaaa
     1381 agctggtacg agcaaaaacg ctaccaaaaa ctgcgcgaaa tcgccaaagg tcgtccgaag
     1441 ttcatcctgc acgacggccc gccgtatgcc aacggcgaca tccacatcgg tcacgccgtc
     1501 aacaaaattc tcaaagacat cattatccgc agcaaaaccc aagccggttt tgacgcgcct
     1561 tatgtgccgg gttgggactg tcacggtttg cctatcgaag tgatggtaga aaagctgcac
     1621 ggcaaagaca tgcccaaagc gcgtttccgc gaattgtgcc gcgaatatgc cgccgaacaa
     1681 gttgcctgcc agaaaaaaga cttcatccgt ctgggcgtgt tgggcgactg ggacaagccc
     1741 tacctgacca tggattttaa aaccgaagcc gataccgtgc gtatgctcgg cgaaatctac
     1801 aaatccggct atctctaccg cggcgcgaaa ccggttcagt tctgcttgga ctgcggctct
     1861 tcattggccg aagccgaagt ggaatacaaa gacaaagtat cgcctgcgat tgacgtgggc
     1921 tatccgttta aagacaccgc tgcgcttgcc gctgcattcg gtttggcagg catcgagggc
     1981 aaagcgtttg ccgtcatctg gacgaccacg ccttggacgc tgccagcgag ccaagccgtg
     2041 tctgccggtg cggacgtggt gtatcaatta atcgatacgc ccaaaggcaa attggtgctg
     2101 gcgaaagatt tggcagaaga cgcgctcaaa cgttacggtt tttcagacgg cattgctatt
     2161 cttgccgaaa ccaccggcga caagctggaa aacctgcaca tgaatcatcc gttcctcgaa
     2221 cgcgatattc ccatgctcaa cggcgaccac gttaccaccg acgccggtac cggtttggtg
     2281 catactgcgc ctgctcatgg tttggaagac tacgccgtct gcaataaata cggcatcgag
     2341 ctttacaacc ccgtcaacgc cgaaggcaaa tacatcagtg aaacgccgcg cgttgccggc
     2401 atgagcgttt gggaggccaa tcccgttatc ctgcaatggt tggaagaaac cggcaacctc
     2461 ttggcaagca gcaaaatcga acacagctac gcgcactgct ggcgtcacaa aacgccgttg
     2521 atttaccgtg cgaccggcca atggtttgtc ggtatggata aagcaggtgc cgatggtaaa
     2581 accctgcgcg ataaagccat caaagccgtt gatgacactg aatttttccc atcatggggt
     2641 cgtgcgcgtt tggaagccat gattgaaggc cgccccgact gggtggtttc gcgccaacgc
     2701 tattggggca cgccgatgac tttctttgtt cacaaagaaa cgggcgagct gcatccgaac
     2761 tctgccgaac ttttggaaaa agtcgcgcaa cgcatcgaag aaaaaggcat cgaggcttgg
     2821 ttctccctcg ataagggcga attgttgagc gcggaagatt gcgaacatta cgacaaactc
     2881 tccgacacga tggacgtatg gttcgactcc ggttcgaccc attattccgt tgtgaaacag
     2941 cgcgaagaat tggactggcc tgctgacttg tacctcgaag gcagcgatca acaccggggc
     3001 tggtttcagt cgtccatgct gaccggctgc gcctcatcaa tgggtcgcgc gccgtacaaa
     3061 cagctgctga cccatggttt cgtggtggat caaaacgggc gcaaaatgtc gaaatccatc
     3121 ggcaacgtcg tcgcgccgca agaagtgtat aacgagttcg gcgcggacat cctgcgcctg
     3181 tgggcggcat ccaccgatta cagcggcgaa ttggcgattt ccaaagaaat cctcaagcgc
     3241 gtaaccgaaa gctaccgccg tatccgcaac accttgagct tcctgtttgc caacctcagc
     3301 gacttcaatc ctattgaaga tgccgtgcaa caggcagaca tggtggaaat cgaccgctac
     3361 gccgtggtat tggcacgtca gctgcaagag cgtctggcag gcgattacta tccgcgctat
     3421 accttccact ttgctgtaaa agacattgtt tctttctgct cggaagactt gggcgcgttc
     3481 tacctcgata tcctgaaaga ccgcctctac accaccaaag ccgacagccg cgcccgccgc
     3541 agcgcgcaaa ccgccctgta ccacatcacg cgcagtttgg ttctcttgat tgcgccgatt
     3601 ttgtgcttta ccggcgaaga agcgtgggac atcatcggcg gcggcgaaga agacagcgtc
     3661 ctcttccaca cctggcacga gttcccgacc atcaacgaaa aagccgaagc cgaactggtg
     3721 aaaaaatgga cggcaatccg cgaagcccgc gaagcggtaa ccgccgccat cgagcctttg
     3781 cgcgccgaca aaaccgtcgg ttcgtccttg caagccgaag ccgaaatcac tgcgccggaa
     3841 gaaatggccg gctacctgaa cgctttgggc gaagaattgc gctttgcctt gctggtgtct
     3901 aaagcagaag tgaaagtagg cagcgaactt gccgttgccg ctaaagccag tgatggtgaa
     3961 aaatgcgaac gctgctggca ctacacccgc gatgtgggcg cggttgcagg ctatgaaacc
     4021 gtctgcaaac gctgtgcaga gaatgtcggc agagaaggcg aaacgcgcca ttacgcctga
     4081 taaagtttga gcaaatgccg tctgaaaccg ccgaacagca tttcagacgg cattttttgt
     4141 gccgcaattt gtctttataa cggcggaggg gtttcaagat tgc
//
LOCUS       DAWKST010000167         4128 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.167, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000167 DAWKST010000000
VERSION     DAWKST010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.167"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            43..675
                     /locus_tag="WJL87_001797"
     CDS             43..675
                     /locus_tag="WJL87_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ7800200.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAVSLFAISDDEDEIVAYLSEHGLQTA"
     gene            747..2117
                     /gene="purB"
                     /locus_tag="WJL87_001798"
     CDS             747..2117
                     /gene="purB"
                     /locus_tag="WJL87_001798"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ7800201.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIVEVPPFSAGTLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKILQAQEFLGKINGAVGNYNAHMA
                     AYPDVDWETHCHNFVEIGLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLAEKLPVSRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKVFIGSLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            2198..4066
                     /locus_tag="WJL87_001799"
     CDS             2198..4066
                     /locus_tag="WJL87_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ7800202.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVIIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTATSFLPSRFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTASGKRQLLSLLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDTTLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFQARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLVKDIPNV
                     HWVDAQKYLPKNTVEIHRRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSRGGALQ"
BASE COUNT          936 a         1113 c         1072 g         1007 t
ORIGIN      
        1 tttttgacgg tgtacaatcg ctgtttttga acggaggatg gaatggagaa tacaaaccgt
       61 gtgccggagc aggcacgtta tgatgccgaa cgcaggcagg cagacgaagc attggcgggc
      121 gtgtttccgg cagtcagtat cttcggcagc gcgcgcacgc cgcagaatca tgcggattat
      181 gcgttcgcct gccgtctggc gcggcggctg tcggattcgg gcattgccgt catttcgggc
      241 ggcgggccgg ggattatgga ggcggcaaac aagggcgcgt ttgcagggaa gtcggtttcg
      301 gtggggctga acatcgtttt gccgcacgag cagaaaccga atccgtatca ggacatcgcc
      361 ttgcggtttt cccgttttgc cgaacgcaag gcggtgtttt tccgctattc ccaagcatat
      421 gtcgtgatgc cgggcggctt cgggacgctg gacgaattgt ttgaaatcct gaccttggtg
      481 cagacgggca aagtgccgcc gcgtccgatt gttttggtcg gaaaggcgtt ttggtcgggc
      541 ttggcggagt ggataaacgc gcagcttttg gcgcgcgggc tgatttccga aggggcggtc
      601 tctttgtttg ccatatcgga cgatgaagac gaaatcgttg cgtatctgtc ggaacacggg
      661 cttcagacgg catagcgtcc tgagagtgat gtataattgc aaacaattta acaatttttg
      721 atgtctttcc cgaacaggat gccgaaatga tcaaccccat cgcctcgctt tcccctttag
      781 acggccgtta tgcccaatcc gttgaagcat tgcgcccgat tttttcagag tacggcctga
      841 tgaaggcgcg cgtcaaagtc gaattaaact ggctcaaagc cctggccgcc gagccgaaga
      901 ttgtcgaagt gccgcccttc agtgccggaa cgcttgccga aatcgacacg gtgattgaaa
      961 acttttcatt ggaagacgcg gccgccgtca aagccatcga agccacgacc aatcatgatg
     1021 tcaaagccat tgaatattgg ctgaaaaaac gttttgccga agtgccggaa gtcgccgccg
     1081 tgagtgagtt catccacttc gcctgcacca gcgaagacat caacaacctg tcccacgctt
     1141 taatgctgca agaagcgcgt gaggctgttt tgctgccgaa gctggccgaa atcatcgaaa
     1201 aactgaccgc tatggcgcac gaccttgccg ccgtcccgat gatgagccgc acccacggcc
     1261 agcccgccac gcccacaaca ctgggcaaag aaaccgccaa cgtcgtgtac cgcctgcaac
     1321 gccagttcaa aatccttcag gcgcaagaat tcctcggcaa aatcaacggt gcggtcggca
     1381 actacaatgc ccatatggcc gcttatcccg atgtggattg ggaaacccac tgccacaact
     1441 tcgttgaaat cggcctcggt ttgaccttca acccctatac catccaaatc gagccgcacg
     1501 actatatggc tgaattcttc caaaccctca gccgcatcaa cacgattctc atcgacttta
     1561 accgcgacgt ttggggttat atttcattgg gttacttcaa acaaaaagtc aaagcgggcg
     1621 aagtcggctc ttccacgatg ccgcacaaag tcaaccccat cgattttgaa aactccgaag
     1681 gcaacctcgg tatggcaaac gccgtattgg gttttttggc ggaaaaactg cccgtctcac
     1741 gctggcagcg cgatttgacc gacagcaccg tcttacgcaa tatgggcgtg ggcgtgggct
     1801 atgccgtgtt gggctttgcc gcccacctgc gcggtctgaa caagctcgaa cccaaccctg
     1861 ccgcgcttgc cgccgatttg gatgccactt gggagctgct cgccgagccg attcaaaccg
     1921 tgatgcgtcg ttacggcgtg gccaatcctt acgaaaagct caaagacctg acgcgcggca
     1981 aaggcggcat tacgcccgaa gtgctgaaag tcttcatcgg atcgttggaa attccggcag
     2041 aagccaaagc aaaattgctt gagctgactc ctgcactgta tgtgggcaag gctgaagcgt
     2101 tggcgaaacg gatttgagcg tttactgaaa ccgatgccgt ctgaacgcgc gttcagacgg
     2161 catttttaag ataacgggac atacgggggc gatatttatg caagctgtcc gatacaggcc
     2221 tgaaattgac ggattgcggg ccgttgccgt gctatccgtc attattttcc acctgaataa
     2281 ccgctggctg cccggagggt ttttgggggt ggacattttc tttgtcatct cgggattcct
     2341 cattaccggc atcattcttt ctgaaataca gaacggttct ttttctttcc gggattttta
     2401 tacccgcagg attaagcgga tttatcctgc ttttatcgcg gccgtgtcgc tggcttcggt
     2461 gattgcctct caaatcttcc tttacgaaga tttcaaccaa atgcggaaaa ccgtggagct
     2521 ttctgcggtt ttcttgtcca atatttatct ggggtttcag caggggtatt tcgatttgag
     2581 tgccgacgag aaccccgtac tgcatatctg gtctttggcg gtagaggaac agtattacct
     2641 cctgtatcct cttttgctga tattttgctg caaaaaaacc aaatcgctac gggtgctgcg
     2701 taacatcagc atcatcctat ttctgatttt gactgccaca tcgtttttgc caagcaggtt
     2761 ttataccgac atcctcaacc aacccaatac ttattacctt tcgacactga ggtttcccga
     2821 gctgttggca ggttcgctgc tggcggttta cgggcaaacg caaaacggca gacggcaaac
     2881 agcaagcgga aaacggcagt tgctttcatt actctgcttc ggcgcattgc ttgcctgcct
     2941 gttcgtgatt gacaaacaca atccgtttat cccgggaatg accctgctcc ttccctgcct
     3001 gctgacggca ctgcttatcc ggagtatgca atacgggaca cttccgaccc gcatcctgtc
     3061 ggcaagcccc atcgtatttg tcggcaaaat ctcttattcc ctatacctgt accattggat
     3121 ttttattgcc ttcgcccatt acattacagg cgacaaacag ctcggactgc ctgccgtatc
     3181 ggcggttgcc gcattgacgg ccggattttc cctgttgagc tattatttga ttgaacagcc
     3241 gcttagaaaa cggaagatga ccttcaaaaa ggcatttttc tgcctctatc tcgccccgtc
     3301 cctgatactt gtcggttaca acctgtacgc aagggggata ttgaaacagg aacacctccg
     3361 cccgttgccc ggcgcgcccc ttgctgcgga aaatcatttt ccggaaaccg tcctgaccct
     3421 cggcgactcg cacgccggac acctgcgggg ttttctggat tatgtcggca gccgggaagg
     3481 gtggaaagcc aaaatcctgt ccctcgattc ggagtgtttg gtttgggtgg atacgacact
     3541 ggcagacaac ccgttatgtc gaaaataccg ggatgaagtt gaaaaagccg aagccgtttt
     3601 cattgcccaa ttctatgatt tgaggatggg cggccagccc gtgccgagat ttgaagcgca
     3661 atccttccta atacccgggt tccaagcccg attcagggaa accgtcaaaa ggatagccgc
     3721 cgtcaaaccc gtctatgttt ttgcaaacaa cacatcaatc agccgttcgc ccctgaggga
     3781 ggaaaaattg aaaagatttg ccgcaaacca atatctccgc cccattcagg ctatgggcga
     3841 catcggcaag agcaatcagg cggtctttga tttggttaaa gatatcccca atgtgcattg
     3901 ggtggacgca cagaaatacc tgcctaaaaa cacggtcgaa atacacagac gctatcttta
     3961 cggcgaccaa gaccacctga cctatttcgg ttcttattat atggggcggg aatttcacaa
     4021 acacgaacgc ctgcttaaat cttcccgcgg cggcgcattg cagtagcctg ccttcttgtc
     4081 ggatattgcc tttggcagcc tatgccgctg tttgcccttc ggggcggc
//
LOCUS       DAWKST010000168         4111 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.168, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000168 DAWKST010000000
VERSION     DAWKST010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.168"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(113..769)
                     /locus_tag="WJL87_001800"
     CDS             complement(113..769)
                     /locus_tag="WJL87_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ7800203.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKTGGDMLQWHTRVG
                     LLILFLLIFRLCWGIWGSDTARFSHFVRGWSGIRDYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRLFKKKNLVFPMITGFKYIEGKTSIHFAGKAALAAALSVVALVAAAILLLS
                     "
     gene            834..1724
                     /locus_tag="WJL87_001801"
     CDS             834..1724
                     /locus_tag="WJL87_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ7800204.1"
                     /translation="MFPVFHLSGESRRRMLQTALRFPYVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDALPFAVIAGFPEIMLDTADGLSKRQD
                     INAWVVGCGLGTGRAAVGTLAGILTEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAIFGATVVLKGHKTLVASPDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITESM"
     gene            1994..3805
                     /gene="typA"
                     /locus_tag="WJL87_001802"
     CDS             1994..3805
                     /gene="typA"
                     /locus_tag="WJL87_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ7800205.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNVRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
BASE COUNT          991 a         1072 c         1194 g          854 t
ORIGIN      
        1 gtttgggacg gaaaaacggc ctgaagccgt ttcgggcatt cagaccgttt gcgcggagag
       61 gggatgccgt ccgaagggcg aaaagggctt cagacggcat tgatgtcggg tttcaggaca
      121 ggagcaggat ggcggctgcg acaagcgcga caaccgataa tgcggcggca agcgcggctt
      181 tgccggcaaa gtgtatggaa gttttgcctt cgatatattt gaagccggtt atcatcggga
      241 agacgaggtt tttctttttg aataggcggt atgcagcgac ggcggcgata tggatggcgg
      301 aaaaaacggc gagcagcttg aaaaagttga ggtggatttt ccgcatcagg ctgcccgtat
      361 gctcggaaac caaatggttg aggtagccgt tggtgctgaa ggtgttttca tcggcggcaa
      421 aaagccccgt gccgacttga aatgagacgg cggccaaaag cgcgacgacc atcagtgcgc
      481 ccaaggggtt gtgtccgggc tggatatgtt cgggaatacc gtttttcaga taatccctga
      541 tacccgacca tccgcggacg aaatgggaaa aacgggcggt atcactgccc caaatgcccc
      601 agcagaggcg gaatatgagc aggaaaagga taagcagccc gacgcgcgtg tgccattgca
      661 gcatatcgcc gccggttttc gcgctatacc acataaaggg tagggatgcg gcaagcagcc
      721 aatggaaaag gcgggtgggg aagtcccaga ctttggtttt gtttttcata atcggtttcc
      781 ggcggtagaa tcggtttgtt ttcgagcctt aattttaacc gattggaggg gcaatgtttc
      841 ccgtttttca tctttcaggc gagagccgcc gccggatgct tcagacggca ttgcgttttc
      901 cctatgtttt caaagcccgt gcggaagatt cgcacaaagg gactttcggc acgctcgccg
      961 tagtcggcgg atcggcaggg atgagcggtg cgcccgtatt ggcggcatcg gcggcgatgt
     1021 atctcggctg cggcaaggtg tgggcgggtt tcaatcagga tgcgctacct tttgccgtta
     1081 ttgccggttt tcccgagatt atgctggata cggcagacgg tttgagcaag cgtcaagata
     1141 taaacgcctg ggttgtcggt tgtggattgg gtacaggtag ggcggcggtc ggaacgcttg
     1201 ccggaatttt gacggaacac acggacaagc ccgtcctttt ggatgcggac gcgctgaaca
     1261 tattatcaac cgatgccgaa acccgaaatc tggcgcgcgg gtgtaaaaac ctgattttaa
     1321 cgccacaccc cgccgaagcc gcgcgcctgc ttggaacgac ggttgcacag gttcaggcgg
     1381 atcggacggc ggcagtgagg aagatagggg caattttcgg cgcaaccgtg gttttaaagg
     1441 ggcacaaaac attggttgcc tcacccgata cggaaatcta tgtcaacgaa agcggcaacg
     1501 cgggattggc aacggcgggc agtggcgacg tattgggcgg catcatcggc agtctgctcg
     1561 cacagggcgt gccggttttt gaagccgcct gcgcgggcgc gtggctgcac ggcgcggcgg
     1621 cggatgtcat aaaagaatcg gcaggcattg cggcagggct gttggcaggg gaaatcgccc
     1681 cggcggcaag gtggctgcgt aaccggataa ctgaaagtat gtaagaagat atagtggatt
     1741 aacaaaaacc agtacggcgt tgcctcgcct tagctcaaag agaacgattc tctaaggtgc
     1801 tgaagcacta agtgaatcgg ttccgtacta tccgtactgt ctgcggcttc gccgccttgt
     1861 cctgattttt gttaatccac tataccgtct gaaaggcaag ggcttcagac ggcatcttca
     1921 tttcccaaat actgtccggt aaagcgtggt ataatgcgta gttatctctt acagtttttg
     1981 aaaaacatta attatgaaac aaatccgcaa catcgccatc atcgcccacg tcgaccacgg
     2041 caaaaccaca ttggtcgacc aactgctgcg ccaatccggc acattccgcg ccaaccagca
     2101 ggttgacgag cgcgtgatgg acagcaacga ccttgaaaaa gaacgcggca tcaccatcct
     2161 cgccaaaaac accgccatcg attacgaagg ctaccacatc aatatcgtcg acacgccggg
     2221 acacgccgac ttcggcggcg aagtggagcg cgttttgggg atggtggact gcgtcgtctt
     2281 gttggtggac gcgcaggaag gcccgatgcc gcaaacccgt ttcgtgacca aaaaagcctt
     2341 ggctttgggg ctgaaaccga ttgtcgtcat caacaaaatc gacaagccgt ccgctcgtcc
     2401 gagctgggtc atcgaccaaa ctttcgagct gttcgacaac ttgggcgcga ccgacgagca
     2461 gttggatttc ccgattgttt acgcttcagg gttgagcggt ttcgccaaat tggaagaaac
     2521 cgacgagagc aacgacatgc gtccgctgtt cgatactatc ttaaaatata cgcctgcacc
     2581 gagcggcagc gcggacgaaa cgctgcaact gcaaatttcc caactcgact acgacaacta
     2641 caccggccgc ctcggtatcg gtcgtatctt gaacggacgc atcaaacccg gccaaaccgt
     2701 tgccgtcatg aaccacgatc agcaaatcgc ccaaggccgc atcaaccagc ttttgggttt
     2761 caaaggcttg gaacgcgtgc cgcttgaaga agccgaagcc ggcgacattg tgattatttc
     2821 cggtatcgaa gacatcggta tcggtgtaac cattaccgac aaagacaacc ccaaaggcct
     2881 gccgatgttg agcgtggacg aaccgacgct gacgatggac tttatggtca acaccagccc
     2941 gctggcgggc acggaaggca aattcgtaac cagccgccaa atccgcgacc gcctgcaaaa
     3001 agaattgctg accaacgtcg ccctgcgcgt ggaagatacc gccgatgccg acgtgttccg
     3061 cgtatccggg cgcggcgagc tgcacctgac cattttgctg gaaaacatgc gccgcgaagg
     3121 ctacgaactc gccgtcggca aaccgcgcgt cgtgtaccgc gacatcgacg gtcaaaaatg
     3181 cgaaccgtat gaaaacctga ccgtggacgt acccgacgac aaccaaggcg cggtaatgga
     3241 agaactcggc cgccgccgtg gcgaactgac taatatggaa agcgacggca acggacgcac
     3301 ccgcctcgaa taccatattc cagcgcgcgg cttgatcggt ttccaaggcg aatttatgac
     3361 cctgacgcgc ggggtcgggc tgatgagcca cgtgttcgac gattacgcgc ccgtcaaacc
     3421 cgatatgccc ggccgccaca acggcgtgct ggtgtcccaa gagcagggcg aggcagtcgc
     3481 ttacgccttg tggaatctgg aagaccgcgg tcgtatgttc gtatcaccca acgacaaaat
     3541 ctacgaaggt atgattatcg gcatccacag ccgcgacaac gatttggtgg tcaacccgct
     3601 caaaggcaaa aaacttacca acgtccgtgc cagcggtacc gacgaagccg ttcgcctgac
     3661 cacgccgatt aagctgacgc tggaaggtgc ggtcgagttt atcgacgatg atgagctggt
     3721 agaaatcacg ccgcaatcca tccgtctgcg caagcgttac ttgagcgaat tggaacgccg
     3781 ccgccatttc aaaaagctag attgatgttt actggattaa tgtttaaatg atacgcgatg
     3841 ccgtctgaaa aatttcagac ggcatttttt attcggacgg gctttgcggc ttcttgaagc
     3901 tgtttcagac ggcgtttttc ctacccaatc aagaaactgc cgccattttt ccagcggtat
     3961 atcgccccgt cgcgtttcgg tatcgggttc ggcttcccgg cagcatgtcg aacaatgccg
     4021 tttgaaggaa tacgcctttt gagtccttac aagcttagga agaggatata gtggattaac
     4081 aaaaatcagg acaaggcgac gaagccgcag a
//
LOCUS       DAWKST010000169         4050 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.169, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000169 DAWKST010000000
VERSION     DAWKST010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.169"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(154..1050)
                     /gene="argB"
                     /locus_tag="WJL87_001803"
     CDS             complement(154..1050)
                     /gene="argB"
                     /locus_tag="WJL87_001803"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ7800206.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRVPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1220..2350
                     /locus_tag="WJL87_001804"
     CDS             1220..2350
                     /locus_tag="WJL87_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ7800207.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFDNAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDHQDATARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGVNLNREWENPTVEKSPEVFFVRERMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANVTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAVLSAILNVAGDLR"
     gene            2423..3274
                     /locus_tag="WJL87_001805"
     CDS             2423..3274
                     /locus_tag="WJL87_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ7800208.1"
                     /translation="MIIHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3357..4019)
                     /locus_tag="WJL87_001806"
     CDS             complement(3357..4019)
                     /locus_tag="WJL87_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ7800209.1"
                     /translation="MDSQKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEDNGTIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
BASE COUNT          878 a         1188 c          993 g          991 t
ORIGIN      
        1 ccagattccc gccttcgcgg gaatgacgga ttttaggttt ctgttttggt ttctgttttc
       61 gcgggaataa caacttagaa attgcccgaa accccaaaaa cagaaaccgg acaaacgggt
      121 tttccgccaa agccggcatt ttccgacttt gcttcaggca tcttccccac cgcccaaaat
      181 catcgaaccg ataccggcat cggtaaagat ttccagcaaa agcgcgttgg gcaccctgcc
      241 gtcgatgata tgcgtggctt tcacgccgtt gacggcggct tcgaccgcag aagcgatttt
      301 cggcagcata ccgccataca gcgtgccgtc ggcaatcagt tcatcaatcc gtttcggcgt
      361 gagtttggtc agcagattgc ccgttttgtc catcacaccg gcgatattcg tcatcatcaa
      421 gagtttttcg gcgttcaatt cttccgccaa tttgcctgct accaaatcgg cgttgatgtt
      481 gaacgcttcg cctttttcac ctacgccgac gggggcgacg acgggaatgc agccacgttc
      541 tatcagccct ttaaccaaac cggtatcgat gctttccacc gtaccgacct gtccgatgtc
      601 cacgccattc tgttcgggcg tatcgatcaa aagtttcttc gccttaatga aatgatcgtc
      661 gcgtccgctc acgccgaccg cgtgtccgcc atatgtgtta atcatcgaca cgatttcttt
      721 attgacatgc ccgcccaaca ccatttcgac aatatccatc gcctctttgt cggtaacgcg
      781 cattccttgg acaaactcac cctttttgcc gactttttca agcatcgcat tgatctgcgg
      841 cccgccgccg tgaacgatga cgggatgaat gccgaccagc ttcagcagca cgacatcgcg
      901 ggcaaaccct tctttcaagg caggttcggt catcgcgttg ccgccgtatt tgatgacggc
      961 gaccgaaccg gaaaaccggc ggatgtaagg cagcgcttcg gcaaggatac gcgccttgtc
     1021 ggcggcggaa ataatgtttt cagactccat aacggctccc tattgttgac aatttcggct
     1081 tatgttaacg ccaaacgccg tctgaaacaa tcttcagacg gcatccggca gactttcggg
     1141 caggttgaag ccttgcgata cgggcggtaa aatcgcggtt tatccggttc gagaaaccgg
     1201 cgacagcaag gactttccca tgattaaaat cagcacccgc ttcgatgccg gctcggtcgt
     1261 cgtcaaagac ctgaccgatc cttccaacat ccgcctcgcc ctgcgtcccg acaacgcctc
     1321 cgattttgca caatggttct acttccggct gcaaggcgcg gcatatcaaa actgcatcat
     1381 gcattttgac aatgcggcag aagccgccta cccgaaaggc tgggaaggct atcaggcgtg
     1441 tgcctcatac gaccgccgca actggttccg cgtaccgact tcctacgaaa acggcgtact
     1501 gaccgtcaac cataccccgc tgtccaatag cgtgtactac gcctatttcg aaccctattc
     1561 cgaagaacag cacctcaacc tcctcggcga cgcgcaaggc agcggcctgt gccgcatcga
     1621 cgacttgggc agcaccgtgc aagggcgcga catcaatctg ctgaccatag gcaaccaggt
     1681 cgaaagcgat ttgaaaatct ggattaccgc ccgccagcat ccaggcgaaa ccatggcgga
     1741 atggtttgtc gaggggctgc tcggcagact gctcgaccat caggatgcaa ccgcgcgcac
     1801 cctgctcgat cgcgccacgt tctatatcgt ccccaacatg aaccccgacg gttcggcact
     1861 gggcaacctt cgcaccaacg ccgccggtgt gaacctcaac cgcgaatggg aaaatccgac
     1921 tgtggaaaaa agccccgaag tgttcttcgt gcgtgaaaga atgctggaaa ccggcgtgga
     1981 tttgtttttg gatatccacg gcgacgaagg gttgcccttt gtctttgttg caggaactga
     2041 aggcgtgccg aactacaatc cgcgcatttc cgcgttggaa acacagttca aaaccgccct
     2101 tttgaacgcc agccccgatt tccaagatga atacggttac gaaaaagatg cgcccggtca
     2161 ggcaaacgtg accttggcaa caaactgggt cggcaaccgt ttcaactgcc ttgcctatac
     2221 gttggaaatg ccgttcaaag acaatgccaa cctgcccgac gacgacttcg ggtggaacgg
     2281 tcaacgttcg ttgcgcttgg gcgaagcggt gctttccgcc attttgaacg tcgccggcga
     2341 tttgcgttga cactccccac tgaaaaatgc cgtctgaaag cccttcccct tttcagacgg
     2401 catttgaaaa cgaaagcctg ttatgattat ccatccccaa ttcgaccccg tccttatcag
     2461 tatcggcccg cttgccgtcc gctggtatgc cctaagctac atcctcggat ttattctttt
     2521 tacctttctc ggcagaaggc gcatcgcgca aggcttgtcc gtttttacca aagaatcgct
     2581 cgacgacttc ctcacttggg gcattttggg cgtaattttg ggcgggcgtt tgggttacgt
     2641 cctgttttac aagttttccg actacctcgc ccatccgctt gatattttca aggtatggga
     2701 aggcggaatg tcgttccacg gcggcttttt gggtgtagtt attgccatat ggttgttcgg
     2761 ccgcaaacac ggcatcggct tcctcaaact gatggacacg gtcgcaccgc tcgttccgct
     2821 gggtctcgct tcgggacgta tcggcaactt catcaacggc gaactttggg gacgcgttac
     2881 cgacatcaac gcattttggg caatgggctt cccgcaggcg cgttacgaag atgccgaagc
     2941 cgccgcgcac aatccgcttt gggcagaatg gctgcaacaa tacggtatgc tgccgcgtca
     3001 tccctcgcag ctttatcagt ttgcacttga aggcatctgc ctgttcgccg tcgtttggct
     3061 gttctctaaa aaacagcggc cgaccggaca agtcgcctca ctcttcctcg gcggctacgg
     3121 catattccgc ttcattgccg aatttgcacg ccaacccgac gactatctcg ggctgctgac
     3181 cttggggctg tcgatggggc aatggttgag cgtcccgatg attgttttgg gtatcgtcgg
     3241 ctttgtccgg ttcggcatga aaaaacagca ctgaccgcct gtgaaaaata ccgtctgaaa
     3301 catggaaatc cggtttcaga cggcattttt acgtttggtt catggaaaca aacgcttcaa
     3361 ttcttaccgc aacacgcctt gtattttctg ccgctgccgc acggacaggg atcgttcctg
     3421 cctgtttttt cgccttccct gcggacggtt tgcggcttgt tgatgaccgc ctgccagtag
     3481 cggtagatat ccgccaatgc ataaggcaac tcggattcca gttccgccag ctcgccttct
     3541 gtgaattgca gacggatagt gccgttgtcc tcttcgtcgt aaataccgcc caatgccatg
     3601 atgggataaa acaactcttc aaacgcttca tcatcgacgg cttcaaacca atcggtcggc
     3661 acaatatcca aaccgtaaag ataagcattg caccatgtgt aaaaatcgct gccgccgtct
     3721 tcgttttcat acagccacaa atcgggcagt tttttatccg acatcgcggc ggttgtttcc
     3781 atcgccattg ccaaaaccag ccgttcgatt tcggaacgtt cggcggcggt aaattgcgat
     3841 tcgtcgccca acacttcggg cagccagtcg agcggtgtca atttgtccgg cccgctcaac
     3901 agcgccgtca taaaaccttg aacctcgtcg caacgcatcg tgttgccttg ttcgcttttg
     3961 gcatccaaca attcgctcaa ccgccgtttg gatgcttcgg taaatttttg ggaatccatc
     4021 attttccttt tcaaatgggt tttgcgccct
//
LOCUS       DAWKST010000170         3925 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.170, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000170 DAWKST010000000
VERSION     DAWKST010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.170"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..331)
                     /locus_tag="WJL87_001807"
     CDS             complement(<1..331)
                     /locus_tag="WJL87_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ7800210.1"
                     /translation="MFSLYREDSLISRLNRDGYLTSPSADFLELLSLCRDIHALTDGA
                     FDPTVQALWKLYADYFAARPDAETPPPQNSVKEALKRVGFGKVSFDDREIRFAEKGMG
                     LSLNGIAQ"
     gene            complement(312..593)
                     /locus_tag="WJL87_001808"
     CDS             complement(312..593)
                     /locus_tag="WJL87_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nosX protein required for nitrous oxide
                     reduction"
                     /protein_id="HEZ7800211.1"
                     /translation="MGRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADDEKRNKDEKRN
                     ENVFFWKGVALGSGAELRLFGVDDRQAADLVNKVLAEVARLEKIVQPLP"
     gene            complement(602..1396)
                     /locus_tag="WJL87_001809"
     CDS             complement(602..1396)
                     /locus_tag="WJL87_001809"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ7800212.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            1780..3114
                     /gene="gdhA"
                     /locus_tag="WJL87_001810"
     CDS             1780..3114
                     /gene="gdhA"
                     /locus_tag="WJL87_001810"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ7800213.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            3256..3630
                     /locus_tag="WJL87_001811"
     CDS             3256..3630
                     /locus_tag="WJL87_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800214.1"
                     /translation="MIMDQYYMELKNKLSNRPILLDNTNDFLFVLVNTVKAMIENTDK
                     SQLSELDKILDGVTSQELKLAYDFCQGKFGQAGFSYRRHPNYFYLSSLIATFPEFELS
                     KADRDYLKGIINFDNYLLYELD"
     gene            3674..>3925
                     /locus_tag="WJL87_001812"
     CDS             3674..>3925
                     /locus_tag="WJL87_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HEZ7800215.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQ"
BASE COUNT         1068 a         1014 c          924 g          919 t
ORIGIN      
        1 cttgcgcgat gccgttcaag gacaaaccca tacccttttc ggcaaaacgg atttcacggt
       61 cgtcaaacga gactttgccg aagccgaccc gtttcagggc ttccttcacg ctgttttgag
      121 gaggcggcgt ttccgcatcg ggacgggcgg caaaataatc ggcatacagt ttccacaacg
      181 cctgcactgt cggatcgaac gcgccgtccg tcagcgcgtg aatatcgcgg cacaggctca
      241 acagttccaa aaaatccgcc gacggcgaag tcagataacc gtccctgttc aggcggctga
      301 tcaggctgtc ttcacggtaa aggctgaaca attttttcca aacgcgccac ttccgccaaa
      361 accttgttga ccaaatccgc cgcctgtctg tcgtccacac cgaacaggcg cagctccgca
      421 ccggaaccca gcgcgacacc tttccagaag aacacatttt cattgcgttt ttcatcttta
      481 ttgcgttttt catcatcggc ggcaaaagga ttcggcagga aagaaaccgc cgcgcccgcc
      541 gccgcaacgg cggcaactgt caaaaaacgc ctgcgcccga aatgcctgcc cataccgcct
      601 cctaaaccga cactgccgcc ttgatgtgcg gatgggggtc gtaaccttcc aactcgaaat
      661 cttcaaactt gaaggaaaac aaatctttga cttcaggatt gattttcatc actggcaagg
      721 cgcgcggttc gcgttccaac tgcaatgcgg cctgctcgaa atggttgcgg tacaaatgcg
      781 cgtcgccaaa cgtatggaca aactcgcccg cctccaatcc gcacacttgc gccatcatca
      841 tggtcaacaa tgcgtagctg gcaatattaa acggcacacc aaggaaaata tctgcactac
      901 gctggtaaag ctggcaggac agtttgccgt cggcaacgta aaactgaaac agcgcgtggc
      961 agggcggcaa ggccatttcg tcgaccaaag ccggattcca cgccgataca atcaggcggc
     1021 gcgagtcggg gtttttctta atttgttcca ccacattggc gatttggtcg atatgcctgc
     1081 cgtcgggcgc gggccagtta cgccactggt agccgtaaac cgggcccaag tcgccgtttt
     1141 cgtccgccca ctcgtcccaa atggaaacat tgttgtcctt taggtatttg atattggtat
     1201 cgcctttgag aaaccaaagc agctcgtgga taatcgaacg cagatgcagc tttttggtcg
     1261 tcagcagcgg aaaacctttg cccaagtcaa aacgcatctg ataaccgaat acggagcgcg
     1321 tgcccgtacc ggtgcggtct gatttgtccg taccgttgtc gaggacgtgg cgcatcaagt
     1381 ccaaataggc tttcatagca gtctttcatc aaattaaacg gcgcatattg taacatttcc
     1441 ggataatgcc caaaacacgg atacagacag gcaggattgt tggcaatttc agtccttttc
     1501 cacagtaaaa cccggtggga aaacaaaatt accttgattg gaatcaaaaa atctagttta
     1561 attacttaga ataaaatttc aataatattt ttatttacga aattaattta tgattattga
     1621 ttaattatcg gcaacaaaca tcaaacatcg aaaacatgga aaaaataatg tcaacaattt
     1681 ttgccaaatc gggcttggca tcagaaaaaa ataggtttat attcccacct acaaatttgt
     1741 tttcccatta gtacactatc aaccaaaagg agtatccgaa tgactgacct gaacaccctg
     1801 tttgccaacc tcaaacaacg caatcccaat caggagccgt tccatcaggc ggttgaagaa
     1861 gtcttcatga gtctcgatcc gtttttggca aaaaatccga aatacaccca gcaaagcctg
     1921 ctggaacgca tcgtcgaacc cgaacgcgtc gtcatgttcc gcgtaacctg gcaggacgat
     1981 aaagggcaag tccaagtcaa ccggggctac cgcgtgcaaa tgagttccgc catcggtcct
     2041 tacaaaggcg gcctgcgctt ccatccgacc gtcgatttgg gcgtattgaa attcctcgct
     2101 tttgaacaag tgttcaaaaa cgccttgacc accctgccta tgggcggcgg caaaggcggt
     2161 tccgacttcg accccaaagg caaatccgat gccgaagtaa tgcgcttctg ccaagccttt
     2221 atgaccgaac tctaccgcca catcggcgcg gacaccgacg ttccggccgg cgacatcggc
     2281 gtaggcggac gcgaaatcgg ctatctgttc ggacaataca aaaaaatccg caacgagttt
     2341 tcttccgtcc tgaccggcaa aggtttggaa tggggcggca gcctcatccg tcccgaagcg
     2401 accggctacg gctgcgtcta tttcgcccaa gcgatgctgc aaacccgcaa cgatagtttt
     2461 gaaggcaaac gcgtcctgat ttccggttcc ggcaacgtgg cgcaatacgc cgccgaaaaa
     2521 gccatccaac tgggcgcgaa agtgctgacc gtttccgact ccaacggctt cgtcctcttc
     2581 cccgacagcg gtatgaccga agcgcaactc gccgccttga tcgaattgaa agaagtccgc
     2641 cgcgaacgcg ttgccaccta cgccaaagag caaggtctgc aatactttga aaaccaaaaa
     2701 ccgtggggcg tcgccgccga aatcgccctg ccctgcgcga cccagaacga attggacgaa
     2761 gaagccgcca aaaccctgtt ggcaaacggc tgctacgtcg ttgccgaagg tgcgaatatg
     2821 ccgtcgactt tgggcgcggt cgagcaattt atcaaagccg gcatcctcta cgccccggga
     2881 aaagcctcca atgccggcgg cgtagcaact tcaggtttgg aaatgagcca aaacgccatc
     2941 cgcctgtctt ggactcgtga agaagtcgac caacgcctgt tcggcatcat gcaaagcatc
     3001 cacgaatcct gtctgaaata cggcaaagtc ggcgacacag taaactacgt caatggtgcg
     3061 aacattgccg gtttcgtcaa agttgccgat gcgatgctgg cgcaaggctt ctaagcaaac
     3121 gccgccgctc cgcaaacaaa atgccgtccg aaccgcaaat gctgttcaga cggcatttca
     3181 ttatccgccc gttcgaatcg ggtgatacta tttgtatcca gaattgttgg atagtttggg
     3241 agtggagagg aagaaatgat tatggaccaa tactatatgg agttaaagaa taagttatct
     3301 aacagaccaa tcctactgga taatactaat gattttcttt ttgttttagt aaatacagtg
     3361 aaagctatga tagaaaatac tgataaaagt cagctttcag agttagataa aattttagac
     3421 ggtgtcacaa gtcaagaatt gaaacttgcc tatgattttt gtcaagggaa attcggtcaa
     3481 gcaggttttt cttatcgtag gcacccgaat tatttttatt tgtccagttt aattgcgact
     3541 tttccagaat ttgagttgtc taaagcagac cgagattacc tcaaaggaat cataaatttt
     3601 gataactacc ttttatatga gttagattag tctttaatca cctcaaaacc ctatttattc
     3661 aaaaggagaa aaaatgattg agctgaaatt agtagatgag agcagttttc aggcagtgct
     3721 ggatttgaaa atatctgaag ctgatgaacg agcacgtttt gtggctccaa atgtacgctc
     3781 tttagctgac gcatggctct accgggaaaa tgaagatgtg tttccgaggg caatctattg
     3841 ggataagcaa gtggttggct ttctcctgct ggaaatagac aaggatgaag cagaatactt
     3901 tatctggcgg ataatgattg gtcag
//
LOCUS       DAWKST010000171         3921 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.171, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000171 DAWKST010000000
VERSION     DAWKST010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.171"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            155..1672
                     /locus_tag="WJL87_001813"
     CDS             155..1672
                     /locus_tag="WJL87_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ7800216.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNRNANNAAPQYCRQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGVAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1731..3512)
                     /locus_tag="WJL87_001814"
     CDS             complement(1731..3512)
                     /locus_tag="WJL87_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ7800217.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHAVLFADYGFGLPLTGVESERGGNLALHWFADCADTDAASWLARHSDGLPAGIST
                     PQSSVSEADYLDHIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVKLYQRLRQPVPYAV
                     LSHLPDAQGQSAWTLCFSPELFLKIGSDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGTVCVPEPFKVSRFGSVWQMTSTIQAQALPHTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLEAEARGLYTGSIGYLNPCSGGLGFEGTFNV
                     VIRTLSLTPLSDGIYQGVYGVGSGIVIDSDPAAEYRECGWKARFLNELRPDFGIFETL
                     RVENGRCALLDRHLCRLKTSAQALNLPLPDGCENQIKQYIARLPDGAFRIKALLASDG
                     ISLSRAVLNRLTDKQRVIISPTILPAQNYLRRFKTTCRTVFDQAWQTAETQGAFDSLF
                     FNSDGILLEGGRSNVFVKYQGQWLTPSLDLDILNGIMRQAVLDEPQKYLQTNQVIETH
                     ITQKTLQEAEEIRLSNALRGVFAAALA"
BASE COUNT          825 a          875 c         1282 g          939 t
ORIGIN      
        1 tataaatgtg aaatccgccc tttgaaaatc gggcggcggt tttttgcctg cccgctttca
       61 ggcggcaaag ccggttttta cgggcttttg cctgtttttc ggatggtttg acgtgcttcg
      121 gcggcgtgtt tgccggaaag gtaaatgaca gggtatgttg tatttcagat acggcttttt
      181 ggttgtttgg tgtgcggcag gtgtttctgc cgcctatggg gcggatgcgc ccgcgatttt
      241 ggatgacaag gcattgttgc aggtgcagcg gtcggtgtcg gataagtggg cggaatcaga
      301 ttggaaagtt gaaaatgatg ccccgcgcgt ggttgacggg gattttttgt tggcacatcc
      361 gaaaatgttg gaacatagtt tgcgcgacgc gctcaacggc aatcaggcgg atttaatcgc
      421 ttcgttggcg gatttgtatg ccaagctgcc ggattatgac gcggttttgt acggcagggc
      481 gcgggctttg ctggcgaaat tggcgggaag gccggcggag gcggtggcgc ggtatcggga
      541 actgcacggg gaaaatgcgg cagacgagcg gattttgctg gatttggcgg cggcggagtt
      601 tgacgatttc cgactgaagt cggcagaaag gcattttgcg gaggcggcaa aattggattt
      661 gccggcaccg gttttggaaa atgtggggcg ttttcggaaa aaaacggagg ggctgacggg
      721 ctggcgtttt tcgggcggca tcagtccggc ggtcaataga aatgccaata atgccgcgcc
      781 gcagtattgc cggcaaaacg gaggccggca gatatgcagt gtcagccggg cggagcgggc
      841 ggcagggttg aattatgaaa tcgaggcgga aaaactgacg gcgttggcag ataatcatta
      901 tttgttgttc cgttccaata tcggcggcac gagctattat ttcagtaaaa aatcagctta
      961 tgacgacggg ttcggcaggg cgtatttggg ttggcagtat aaaaatgcac ggcagacggc
     1021 ggggattttg ccgttttatc aggtgcagtt gtcgggcagc gacggctttg atgcgaaaac
     1081 aaaacgggta aacaaccgcc gcctgccgcc gtatatgctg gcgcacggag tcggcgtgca
     1141 gctgtcccat acttaccgac caaacccggg atggcaattt tcggtcgcgc tggaacatta
     1201 ccgccaacgc taccgcgaac aggatagggc ggaatacaat aacggcaggc aggacgggtt
     1261 ttatgtttcg tcggcaaaac gtttgggcga atcggcaact gtgttcggcg gctggcagtt
     1321 tgtgcggttt gtgccgaaac gcgaaacggt gggcggcgcg gtcaataatg ccgcctaccg
     1381 gcgcaacggt gtttatgccg gctgggcgca ggagtggcgg cagttgggcg gtttgaatag
     1441 tcgggtttcc gcgtcttatg cccgccgcaa ctataagggc gttgcggctt tctcgacaga
     1501 ggcgcaacgc aaccgcgaat ggaatgtctc gctggctttg agccacgaca agttgtcgta
     1561 caaaggtatc gtgcccgcgt tgaattatcg tttcggcagg acggaaagta atgtgccgta
     1621 tgcgaaacgc cgcaacagcg aggtgtttgt gtcggcggat tggcggtttt gaatggtggg
     1681 ataatgccgt ccgaactttg gaaacaggtt cggacggcat ttttgcgcgt tcaggcaagg
     1741 gcggcggcaa atacgccgcg caaggcgttg gagaggcgga tttcttcggc ttcttgcagt
     1801 gttttttgtg tgatgtgtgt ttcgattact tgatttgttt gcaaatattt ttgcggttcg
     1861 tccaatacgg cttggcgcat tatgccgttt aaaatgtcta aatctaaaga gggcgtaagc
     1921 cattgtcctt ggtatttgac gaacacgttg cttctgccgc cttcgagcag gatgccgtct
     1981 gaattgaaaa acaggctgtc gaacgcgcct tgtgtttcgg cggtttgcca cgcttggtcg
     2041 aagacggtgc ggcaggtggt tttgaagcgg cgcaggtagt tttgtgcggg caggatggtc
     2101 ggcgaaatga tgacgcgttg tttgtcggtc agacggttta aaacggcgcg ggacaggctg
     2161 atgccgtctg aagcgagcag ggctttgatg cggaacgcgc cgtcgggcaa gcgtgcgatg
     2221 tattgtttga tttgattttc gcaaccgtcg ggcaggggca ggttgagggc ttgggcggag
     2281 gttttcagac ggcataggtg gcggtcgagc agggcgcagc gtccgttttc cacgcgcagg
     2341 gtttcaaaaa tgccgaagtc ggggcgcaat tcgttgagga aacgggcttt ccagccgcat
     2401 tcccgatatt cggcggcagg gtcgctgtcg atgacgatgc cggaaccgac accgtacacg
     2461 ccttgataaa tgccgtctga aagcggcgtg agcgacaagg tgcggataac gacgttgaac
     2521 gtgccttcaa accccaagcc gccggaacac gggttcaaat agccgatgct gcccgtataa
     2581 agtccgcgcg cttcggcttc gagcgattcg ataatctgca tactcatttt tttgggcgcg
     2641 ccggtgatgc tgccgcaggg gaaggcggcg cggaggatgt cggcgaacga ggtgtgcggc
     2701 aaggcttggg cttggatggt gctggtcatc tgccaaacgc tgccgaaacg cgatacttta
     2761 aacggttcgg gtacgcatac tgtgccggtt tgggcgattt tgccgaggtc gttacgcagc
     2821 aaatcgacaa tcatcacgtt ttcggcgcgg tttttcgggt ctgcttgcaa ctcggcggcg
     2881 cggcgttcgt cttgtccgtc gcccaaaatc ggcgcggtgc ctttcatcgg ttcggtgctg
     2941 atggtgccgt ccgaaccgat tttgaggaag agttcgggcg agaaacacag cgtccacgcg
     3001 gattgccctt gtgcatcggg caggtgggac aaaacggcat aggggacggg ctggcgcagg
     3061 cgttggtaaa gtttgacggg attgccgtag gcttgcaggt gcaggcgggt agtgtagttg
     3121 atttgatagg tgtcgccgcg tcggatggct tcgtggattt ggcggatatg gtcgaggtaa
     3181 tcggcttcgg atacggagga ttgcggcgtg gaaatgccgg cggggaggcc gtctgagtgt
     3241 cgggcaagcc agcttgcggc atcggtgtcg gcgcagtcgg caaaccagtg cagggcaagg
     3301 ttgccgccgc gttcggactc gacccccgtc agcggcaaac cgaatccgta gtctgcaaac
     3361 aacacggcat gcagcccttt ttgccagccc gattgcagcg cgccgtccaa agcatcgagt
     3421 tcttcgggac ggaaaaaacg gctttccaca tgattttgat agcgttttgc gcggccgctt
     3481 acggcatcgt caaacagggc gaaataaggc atggcaattc ggggcaaatg ttttgattat
     3541 acgctctttt tacacatatt ttcagacgat tcggcaaata tcggcaaaat gtaattttat
     3601 gtagagaaag tggggggcga aggtgtaaaa ttgtgaaaac aaattccgat ttccaacctg
     3661 aaatacaata aggagacctt tatgacagac caccagctgc aaccgtttga aaacgtagaa
     3721 ttaggcgaaa agcaagatca gctccaagta tagtggatta acaaaaacca gtacagcgtt
     3781 gcctcgcctt ggctcaaaga gaacgattct ctaaggtgct gaagcaccaa gtgaatcggt
     3841 tccgtactat ctgtactgtc tgcgtcttcg tcgccttgtc ctgatttttg ttaatccgct
     3901 ataaagaccg tcgggcatct g
//
LOCUS       DAWKST010000172         3919 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.172, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000172 DAWKST010000000
VERSION     DAWKST010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.172"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            128..2713
                     /gene="acnB"
                     /locus_tag="WJL87_001815"
     CDS             128..2713
                     /gene="acnB"
                     /locus_tag="WJL87_001815"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ7800218.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFTSRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIADGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            2855..3850
                     /locus_tag="WJL87_001816"
     CDS             2855..3850
                     /locus_tag="WJL87_001816"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ7800219.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDG
                     IEYRGFAQETVEELTKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPASIVFDQAENRMHTIKAVMVAALGD"
BASE COUNT         1002 a         1140 c          992 g          785 t
ORIGIN      
        1 attacctgtc ataacagttc catttttgtt aaaactagtc tatgatagcg gtacaaatat
       61 tgtttacaat atttaacgca aatcatttgc aacccgacaa aagaaaaaca gaaaaaggaa
      121 caaagagatg ttagaagcct atcgtaaagc cgccgccgag cgcgccgccc tcggcattcc
      181 cgccctccct ttgaacgcgc agcaaaccgc cgatttggtt gagctgctga aaagcccgcc
      241 cgcaggcgaa ggcgagttct tggtcgaact gcttgcccac cgtgttccgc ccggtgtgga
      301 cgatgccgcc aaagtcaaag cctcattcct ggctgccgtt gccgaaggca gcgcgtccag
      361 cccgctgatc tcccccgaat atgcgaccga actcttaggt acgatgctcg gcggttacaa
      421 tatccacgcc ttaatcgaac tcttggacga cgacaaactc gcgcccattg ccgccaaagg
      481 cttgaaacat acgcttctga tgttcgattc cttccacgac gttcaggaaa aagccgaaaa
      541 aggcaacaaa tacgcgcaag aagttttgca atcttgggca gatgccgaat ggtttacctc
      601 acgcgccaaa gttcccgaaa aaatcaccgt taccgttttc aaagttgacg gcgaaaccaa
      661 tacagacgac ctctcccccg cgcccgacgc gtggagtcgt cccgatattc cgctgcacgc
      721 gctggccatg ctgaaaaacc cgcgcgacgg catcacgccc gacaaaccgg gcgaagtcgg
      781 tccgattaaa ttgttggaag aactcaaagc caaaggccat ccggttgctt acgtcggcga
      841 cgtggtcggt actggttctt cacgcaaatc cgcgaccaac tccgtcattt ggcataccgg
      901 cgaagacatt ccgttcgtgc cgaacaaacg cttcggcggc gtgtgcttgg gcggcaaaat
      961 cgcgccgatt ttcttcaata cccaagaaga ctccggcgcg ctgccgattg aagtcgatgt
     1021 ttccgctcta aaaatgggcg atgtcgtcga tatcctgcct tatgaaggca aaatcgtgaa
     1081 aaacggcgag actgttgccg agtttgaatt gaaatcacaa gtattgctgg acgaagtgca
     1141 agccggcggc cgtatcaacc tgattatcgg ccgaggtctg accgccaaag cgcgcgaagc
     1201 cctgaaactg cctgcctcta ctgcattccg cctgccgcaa gcgcctgccg aaagcaaagc
     1261 cggtttcacc ttggcgcaaa aaatggtcgg ccgcgcctgc ggtcttcccg aaggacaagg
     1321 cgtgcgtccg ggtacttact gcgaaccgcg tatgacgacg gtcggctcgc aagacacgac
     1381 cggcccgatg acccgcgacg agttgaaaga cttggcttgt ttgggcttct ccgccgatat
     1441 ggtgatgcag tctttctgcc acaccgccgc ctatccgaaa cctgtcgatg taaaaaccca
     1501 taaagaactg cccgccttta tttccacccg tggcggcgtg tccctgcgtc cgggcgacgg
     1561 cgtcatccac tcgtggctca accgcctgct gctgcccgat accgtcggca ccggcggcga
     1621 cagccacacc cgtttcccca tcggtatttc cttccccgcc ggctctggct tggttgcctt
     1681 tgccgccgca acaggcgtaa tgccgctcga tatgcccgag tctgtattgg tacgcttcag
     1741 cggcaaactg caaccgggtg taaccctgcg cgatttggtg aacgccattc cgctgtacgc
     1801 aatcaaacaa ggtttgctga ccgttgccaa agccggtaag aaaaacatct tctccggccg
     1861 catcctcgaa atcgaaggcc tgcctgattt gaaagtggaa caagcctttg aattgaccga
     1921 cgcatccgcc gaacgctccg ccgccggctg taccgtgaag ctcaacaaag agccgattat
     1981 cgagtacatg aaatccaacg tcgtgttgat gaaaaacatg attgccgacg gctatcaaga
     2041 cccgcgtact ttggaacgcc gcatcaaagc catggaaaaa tggctggcaa atcccgagtt
     2101 gctcgaagcg gataaagatg ccgaatacgc cgccgtgatt gaaatcaaca tggacgacat
     2161 caaagagccg attatcgcct gcccgaacga cccggacgac gtgtgcttca tgtccgaacg
     2221 ctccggcacc aaaatcgacg aagtattcat cggttcgtgt atgaccaaca tcggccactt
     2281 ccgcgccgcc tccaaacttt tggaaggcaa ggcagacacc cccgtccgcc tgtggattgc
     2341 gccgccgacc aaaatggacg cgaaacaatt gtccgacgaa ggacactacg gcgtactcgg
     2401 acgtgccggc gcgcgtatgg aaatgccggg ttgctcctta tgtatgggta atcaggcgca
     2461 agtacgcgaa ggtgcgaccg ttatgtccac ctccacccgc aactttccga accgtttggg
     2521 taaaaacacc tttgtttacc tcggttcggc ggaattggca gcgatttgct ccaaactggg
     2581 taaaatcccg accgttgaag aatatcaagc caatatcggc atcatcaacg aacagggcga
     2641 taaaatctac cgctatatga acttcaacga aatcgacagc tacaacgaag tagccgagac
     2701 cgtgaacgtt taatccccgt catccgtatg aagtaaggga ttgaccgcaa tgccgtctga
     2761 acaaccttca gacggcattg caacattccg ctaacccttc tttccgcaaa cgctgcaaat
     2821 acggcgttca cgcccccaca taaaggaaac gacagtgaac ctgaaaaacc gccattttct
     2881 gaaactttta gacttcacgc cagaagaaat caccgcctac ctcgaccttg ccgccgaatt
     2941 gaaagccgcc aaaaaagcag ggcgcgagat tcagcggatg aaagggaaaa acatcgccct
     3001 gatttttgaa aaaacctcta ctcggacgcg ctgcgcgttt gaagtcgccg cgcgcgatca
     3061 aggcgcggga gtgacttatt tagagccgtc cgccagccaa atcgggcata aggaaagcat
     3121 caaagacacc gcccgcgtgt tgggcaggat gtacgacggc atcgaatatc gcggcttcgc
     3181 tcaggaaact gtcgaagaat tgacgaaata cgcgggcgta cccgtgttca acgggctgac
     3241 caacgagttc catcccacac aaatgcttgc cgacgcactg actatgcgcg aacacagcgg
     3301 caaacctttg aaccaaaccg cgtttgccta cgtcggcgac gcgcgttaca acatgggcaa
     3361 ttccctgctg attttagggg caaaattggg gatggacgtg cgtatcggcg caccgcaaag
     3421 cctgtggccg tctgaaggca ttattgccgc cgcacacgcc gccgccaaag aaaccggcgc
     3481 aaaaattacc ctgaccgaaa acgcgcatga agccgtgaag aatgttgatt ttattcatac
     3541 cgatgtgtgg gtcagcatgg gcgagccgaa agaagtctgg caggaacgca tcgatttgct
     3601 gaaagattac cgcgttacgc ccgaactgat ggcggcatcg ggcaatccgc aagtcaaatt
     3661 catgcactgc ctgcccgcct tccacaaccg cgaaaccaaa gtcggcgaat ggatttacga
     3721 aaccttcggg ctgaacggtg tggaagttac agaagaagta ttcgaaagcc ccgccagcat
     3781 cgtgttcgat caggcggaaa accgtatgca cacgattaaa gcggtaatgg tcgcggcttt
     3841 gggcgactga cagaactgtg cctgtttaaa ttcatccgca acacagatac cgtctgaaca
     3901 cgatgttcag acggtatcc
//
LOCUS       DAWKST010000173         3898 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.173, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000173 DAWKST010000000
VERSION     DAWKST010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.173"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(267..2699)
                     /gene="hupB"
                     /locus_tag="WJL87_001817"
     CDS             complement(267..2699)
                     /gene="hupB"
                     /locus_tag="WJL87_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HEZ7800220.1"
                     /translation="MPIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDIEIKNDGYVYNPTDTGGPSKYLTYVATGVARSQPDPQEWVNKSTLFKL
                     GYNFNDQNRIGWIFEDSRTDRFTNELSNLWTGTTTSAATGDYRHRQDVSYRRRSGVEY
                     KNELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDLRGINGEVYHSFRHIRQNTAQWT
                     ADFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFAKLYAPKILASNQAKITMLIENR
                     SKYKFAYWNNAFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAH
                     AGFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPNLKAEKAK
                     NWELGLAGSGKAGNFKLSGFKTKYRDFIELTYMGVSSDDKNNPRYAPLSDGTALVSSP
                     VWQNQNRSAAWVKGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGKATQNNGKETPINAL
                     SPWTAVYSLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLF
                     DLSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVDNKTHAGIQRFTSP
                     GRSYNFTIEAKF"
     gene            complement(2730..3773)
                     /locus_tag="WJL87_001818"
     CDS             complement(2730..3773)
                     /locus_tag="WJL87_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein"
                     /protein_id="HEZ7800221.1"
                     /translation="MLPPLPPVPGGGVAEPHVPVSIPTATPLTGEVKLSSDNSKIENI
                     NTANTETHPPRRTRRSLYASPQNTSSGISIQQREVEKIYFGVKSPEKSFIFQTPGGAQ
                     YALSSYADPIVPSYSSPDFKIPDRHAGQRLADGSRIFICCSDSGATSYAEITKQDYMK
                     FGAWIGPNGEIDLFAGGFPIGKTPPPAFSWGSPTTETALSKGKITYQVWGIRVRNGQF
                     VTSSYTPPKGSSWSGYQNTPVLSFITANFNSNKLAGEIRGNSDYGPDVEIKEAQIDGL
                     SFSGDATSGGKTGKLEGKFFGKFNGYRDTETSIGGKITFDGDRSLDTVFGGVSYKKEL
                     ENNTDTSTTHLTK"
BASE COUNT          729 a          902 c         1218 g         1049 t
ORIGIN      
        1 ggtgcaggtt tctgtgcgga tagcttcgtc attcccgcgc aggcgggaat ccaggttcgt
       61 tgagtttcag tcatttccga taaattcttg cagctttgcg tttctagatt cccacttccg
      121 tgggaatgac ggagagtcgc gggaatgacg gagagttgcg ggaatgggct tggtacaagc
      181 ctgaaagggg gcggcggtgc gaccgtaaaa aatgccgtct gaaacccaaa tcatcgggtt
      241 tcagacggca ttggcacatc aggagcttag aacttcgctt cgatggtgaa attgtagctc
      301 ctgcccggcg aggtaaagcg ttggatgccg gcgtgggttt tgttgtcaac gcggttgacc
      361 gtgccgaact cgcggatgct gcgtaaagat tcccaagtgt agtactgctt gttggtaatg
      421 ttgtacgcgg cggcgcggag cgtaacctgt ttgccgatgt tgaggtaggc ggaaaggtcg
      481 aacagcgtat aggccttgct gtgtttggca taaggccacg ggttgttcaa gtcgtcgttg
      541 ctgtggacgg tgtcggacgg ctttttggcg gcggtgcgcg tggcgtaggc gttgatgccc
      601 caacgtttgg aaggagcgtc gtagcccagg ctgtaaaccg ccgtccacgg cgaaagcgcg
      661 ttgatgggcg tttctttgcc gttgttttgc gttgccttgc ccttgatgta gctgacgttg
      721 aggccggtgt gcagcccttt gggcaaaccg atactgtcga ggttccacgt gccgttaaac
      781 tctatgcctt tcacccaggc ggcggagcgg ttttggtttt gccaaacggg cgagctgacc
      841 aatgccgtac cgtctgaaag cggggcgtat ctggggttgt ttttatcgtc tgacgaaacg
      901 cccatatacg tcaattcgat aaagtcgcgg tatttggttt tgaagcccga gagcttgaag
      961 ttgcccgctt tgccgctgcc cgccagaccc aattcccagt ttttggcttt ttccgctttc
     1021 aggtttgggt tggctttcaa gtagaaatcg gggtgtggga acagcagcca ggtttcgtcc
     1081 gaagtcggtg cgcggaagcc ggtgctgtat tttgccaaca agtgcagatg cttggtaaac
     1141 cgccagtcga accccgtgcc gtagctgaag cccgcgtgcg cgcgttccga acccaaatgg
     1201 ggaatctgcc cccggatggc ggtggtgtat ttcggatcgt ctttcgcgct gctgctgttt
     1261 ttgtcgtagc gtatgcccgc gttcaggcgg aagcggtcgt tgccgcccaa gtgaaacgca
     1321 ttgttccaat aggcaaattt atatttcgac cggttttcga tcagcattgt gattttggct
     1381 tggttggaag cgaggatttt gggggcgtac agttttgcga aatagctgta atccgagttg
     1441 gcattgtccc ctctgccgcc gcccaagccg tattgcgccg cccaaacggc tttggaaaag
     1501 tcgagttgtt tttcaaaatc ggcagtccat tgcgcggtgt tttggcggat atgccggaac
     1561 gaatggtaaa cctcgccgtt gatgccgcgc aaatcgtaat ttttcgggat gtcccaagtc
     1621 caagtgttca tatcgatgcg ctgcttgtcg tagcgcagct tgaggctgtc ccacgggccg
     1681 tgttccaatt cgtttttgta ttcgacaccg gagcggcggc ggtagctcac gtcttggcgg
     1741 tggcggtagt cgcccgttgc ggcagacgtg gtcgtacccg tccacaaatt agacagctcg
     1801 ttggtaaaac ggtcggtgcg cgagtcttca aaaatccagc cgatacggtt ttgatcgttg
     1861 aagttgtagc ccagcttgaa cagggtgctt ttgtttaccc attcttgcgg gtcgggttgg
     1921 gagcgcgcaa cccctgtggc tacataggtc aggtacttgc tgggtccgcc tgtatcggtc
     1981 gggttataga catatccgtc gtttttaatt tcgatatcgc cctcggtcga gcggtttttg
     2041 gtttctttgc cgaagcggcg ggtataaacc aataaggcat ccaaaccaaa gagcctgccg
     2101 gcggcggtga tgctgctgaa tttttggctg tttttgccga cgctgccgcc ctttatcccc
     2161 aaatggtagg gcttgtcttc ggaaacataa tcgcttgcgg atttggtttg gtaattgact
     2221 gcgccgccca atgcgccgct gccggatttg agcgagtccg cgcctttggt gatggttact
     2281 tcggaaaagt tttccggctc ggaagtgttg cggttggcgt tgaagttgcc gtacgcgccg
     2341 aacaattctt ggaaggcttc ggaagagcgg ctttccgcct gcgccagccc gtcaacgttg
     2401 atggcgacgc ggtctttgtc cacgccgcgt atggtaaagc cgttagaacc cgccctgccg
     2461 ccttcgacga cggaaatgcc ggggtcgtag cgcaccaggt cgtgttcgtc gttgaccaag
     2521 agcttgtcta aagttttgcg gcggattttt tcttcgccga gtttttgggt tttgtgcgtg
     2581 ccggtaacgg tgatttcgtt cagcgtttgg gcggactgcg gcgcggggtc tgccgcaaag
     2641 gcgggaaacg cttgggcgat ggcggcggca gccaatacgg gtttgaaggg gatgggcata
     2701 agaaaacctt tttaaaagcg gattgattat tattttgtga gatgggtggt acttgtatca
     2761 gtgttgtttt caagttcttt tttataactc acaccgccga atacggtatc gagggagcgg
     2821 tcgccgtcaa aggtgatttt gccgcctatg ctggtttcag tgtcccggta gccgttgaat
     2881 tttccaaaaa acttgccttc caatttgccg gttttgccgc cggaggtggc atcgccggaa
     2941 aaggataatc cgtcaatctg tgcttcttta atttccacat ccggcccgta atcgctgttg
     3001 ccgcggattt cgcctgccag tttgttgctg ttgaaattgg cggtgataaa ggaaaggacg
     3061 ggggtgtttt gataaccact ccaagagctg cctttgggcg gcgtataaga ggaagtaaca
     3121 aattgcccgt ttctgacgcg gatgccccaa acctgataag tgattttgcc ttttgacaat
     3181 gcggtttcgg tcgtggggct gccccaggag aacgcaggag gcggcgtttt gccgatgggg
     3241 aagccgccgg cgaagaggtc gatttcgccg ttggggccta tccacgcccc gaatttcata
     3301 taatcttgtt tggtaatttc cgcatacgaa gtagccccgg agtcgctgca gcagataaag
     3361 atgcggctgc cgtcggcaag ccgctgccct gcgtggcggt cgggaatttt gaagtcgggg
     3421 gaggagtagc tggggacgat ggggtcggcg taggatgaaa gggcgtattg cgcgccgccg
     3481 ggtgtttgga agatgaatga tttttctggt gatttaacac cgaaatagat tttttctact
     3541 tcccgctgct gaatggaaat accggaggat gtgttttgcg gggaggcata gagggagcgt
     3601 ctggtgcggc gcggcggatg tgtttcagtg ttggcggtgt tgatgttttc gattttgctg
     3661 ttatcgcttg ataactttac ctcaccggtc agcggcgtgg cggtggggat ggacacgggg
     3721 acgtgcggtt cggctacccc cccccccggc acaggcggca agggcggcag caagcggcag
     3781 taaggagagg gctttgtatt tcatcgcata ctcaatcttg atgatgtgtt aataatagtt
     3841 ttgattatca gtttaaatgt tatatgatgt caactggttt tgtgggtcgg tgcaaaaa
//
LOCUS       DAWKST010000174         3888 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.174, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000174 DAWKST010000000
VERSION     DAWKST010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.174"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            572..982
                     /locus_tag="WJL87_001819"
     CDS             572..982
                     /locus_tag="WJL87_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ7800222.1"
                     /translation="MNHIARQFAILLGEGLKPLGIAPAQFPILLELWQKDGLSQQELV
                     ERADLKQATIANTLARMERDGLITREPNPDDARSRLIMLTEQARALQQQSTEIAQAIN
                     QTALSDLSANEQKLFLEMAAKIVARQQEMITGEK"
     gene            complement(1097..1648)
                     /locus_tag="WJL87_001820"
                     /pseudo
     CDS             complement(1097..1648)
                     /locus_tag="WJL87_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520560.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(1689..2348)
                     /gene="ung"
                     /locus_tag="WJL87_001821"
     CDS             complement(1689..2348)
                     /gene="ung"
                     /locus_tag="WJL87_001821"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ7800223.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPAADVFNA
                     FRLTAFDRVKAVILGQDPYHGAGQAHGLAFSVRQGIRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKGRLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            2429..2836
                     /locus_tag="WJL87_001822"
     CDS             2429..2836
                     /locus_tag="WJL87_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ7800224.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDAGQYVEILRHTGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIIANP"
     gene            2851..3798
                     /locus_tag="WJL87_001823"
     CDS             2851..3798
                     /locus_tag="WJL87_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ7800225.1"
                     /translation="MEFFIILLVAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
BASE COUNT          956 a          958 c         1001 g          973 t
ORIGIN      
        1 atcgaatgcc cggatgcgtg attattttgc cgaaaacgat actgccgaaa acggtatccg
       61 gacggcttga tttgagtttt ggtatttttg cccgacgggg tgaaaaatac agttgctacg
      121 cgccaaacag acaatatcag cagcaggatt ccgactgctt tgtgcgaatt gattagaaat
      181 ttcaggtttt cgtcgatatt ccacgcaatg acggtagcga acataaacag gtagcaagct
      241 gctattgtcc aatgcaggaa gcgggttgtt gagccgtagc tttgcaaagt gtcgggtttc
      301 atgatgcttt ctcttgataa agggttagtc ggatttcggt atggcttaca gataggaaaa
      361 ccttctttaa agtattgaag ttcataagtg ttcttgaaca tttaagatag gtcgtctgaa
      421 agtgtttaga tagcaagcta ttaagctgca ataagtatag cgtgctatgt attaagatag
      481 caagctatat ttgtgatata atccgcaaca atttgatttg aaagaaaaca aagtggacga
      541 agttttcaaa actgaatcgg caggctattt gatgaaccac attgcccgcc aatttgccat
      601 tttattgggc gaaggtttaa agcctttggg catcgccccc gcgcaatttc cgatattgct
      661 ggaactgtgg caaaaagacg gtttgagcca gcaggaattg gtggaacgcg ccgatttgaa
      721 gcaggcaacg attgccaaca ctttggcgcg gatggagcgc gacggactga ttacgcgcga
      781 accgaatccc gacgatgcgc gcagccgcct gattatgctg acggagcaag ctcgcgcctt
      841 gcagcagcag tccacggaaa ttgcccaagc catcaaccaa acggctttgt cggatttgtc
      901 ggcaaacgaa caaaagttgt ttttggaaat ggcggcaaaa attgtggcgc gacagcaaga
      961 gatgattacg ggcgagaagt gagggggccg atggcgcaac ttggaatttt aaagtgcagg
     1021 ctgcttttga ggtcgtctga aagcagcctg cgctttgaaa acaaggtgca ggctgcttag
     1081 ggtaatgtgt cgtttatcaa tccagtttca ggatttctcc gtcttccgga acgttcacac
     1141 ggctttcaat gccttgaccg cgtataaatt tacgcatatc ggtgcggctg acggcggtat
     1201 gattcacggt atccatgtgt acggtaatgc ctgaaatcag cgcgtagccc gtgttcataa
     1261 tcagataatc gggtttgtag cggttcaatg ctttgtttac atctgccgtc cacacagtat
     1321 cacccataat ataagcggtt ttgtgtccgc tgctttggaa tactacaccc attgcatcac
     1381 ctaagatttc ggctagctgc gggttggcat acatggcttc tgtaccatgt acgccgccgg
     1441 ttttgctgat ggttacgccg ttgaagacag tgctgccgtt caacacgcgt acatcagtaa
     1501 atccttggct gcggattttt gccgcgtcgg cttggtgctg cacatatacg ggcaggtttt
     1561 tcggaatgga acgtgcggcg gtttcgcccc aatggtcttc gtgggtatgg gtaacgataa
     1621 ctgcatccac accgtagatt gactggttcc ataagtgcag tttaagcaac ttggcattca
     1681 gacggcattc acagcttcca gtttatcgga tcgataccgt gccggctcaa atagctgttt
     1741 gcctgtgaaa aatggcggca gccgaaaaaa ccgcgatatg ccgacagagg agacggatgc
     1801 ggtgcggtca atatcaaatg attttgactg tctatcagcc tccctttttg ttgtgcatac
     1861 ccaccccaca acatgaagac aaggtgcttg cggtgtgtcg caagctgcct gatgacggta
     1921 tcggtaaagc gttcccagcc taaaagggcg tgcgaatgcg cctgtcctgc acgcaccgtc
     1981 aaaaccgtgt tcagaagcaa tacgccctgc tccgcccacg ctgtcagaca gccgtgcgcg
     2041 ggaatggaaa agccttcgat gtcggtttcc aactccttgt agatattgag taaagacggc
     2101 ggtatgcgga taccctgccg gacggaaaat gccaaaccgt gcgcctgccc tgccccgtga
     2161 tacggatctt gtccgagaat gacggctttg acccggtcga acgctgtcag gcggaatgcg
     2221 ttgaacacat ccgccgccgg cggatagatg atttgtcccg acaaacgttc ctgcctgact
     2281 gcatttaaaa tttcctgaaa atacggctgc tgtttttcgc cgccgagtgc atcgtgccaa
     2341 gtgtccatat ctgccctatc ctttcaaatt cggctatact gctgatattg cgttattttg
     2401 tcaaacgacg cgctttgtgg ggaaatgtat gactgtatgg tttgttgccg ctgttgccgt
     2461 cttaatcatc gaattattga cgggaacggt ttatcttttg gttgtcagcg cggctttggc
     2521 gggttcgggc attgcttacg ggctgaccgg cagtacgcct gccgccgtct tgaccgccgc
     2581 tctgctttcc gcgctgggta tttggttcgt acacgccaaa accgccgtgg gaaaagttga
     2641 aacggattca tatcaggatt tggatgccgg acaatatgtc gaaatcctcc gacacacagg
     2701 cggcaaccgt tacgaagttt tttatcgcgg tacgcactgg caggctcaaa atacggggca
     2761 agaagagctt gaaccaggaa ctcgcgccct aatcgtccgc aaggaaggca accttcttat
     2821 catcgcaaac ccttaacact cggaggaatt atggaatttt tcattatctt gttggtagcc
     2881 gttgccgttt tcggcttcaa atcctttgtc gtcatcccac agcaggaagt ccacgttgtc
     2941 gaaaggcttg gacgtttcca tcgcgccttg acggccggtt tgaatatttt gattcccttt
     3001 atcgaccgcg tcgcctaccg tcattcgctg aaagaaatcc ctttagacgt acccagccag
     3061 gtctgcatca cgcgcgacaa cacccagctg acggttgacg gcatcatcta tttccaagta
     3121 accgatccca aactcgcctc atacggttcg agcaactaca ttatggcgat tacccagctt
     3181 gcccaaacga cgctgcgttc cgttatcggg cgtatggagt tggacaaaac gtttgaagaa
     3241 cgcgacgaaa tcaacagcac cgtcgtctcc gccctcgatg aagccgccgg agcttggggc
     3301 gtgaaggttt tgcgttatga gattaaagac ttggttccgc cgcaagaaat ccttcgctca
     3361 atgcaggcgc aaattactgc cgaacgcgaa aaacgcgccc gtatcgccga atccgaaggt
     3421 cgtaaaatcg aacaaatcaa ccttgccagt ggtcagcgcg aagccgaaat ccaacaatcc
     3481 gaaggcgagg ctcaggctgc ggtcaatgcg tcaaatgccg aaaaaatcgc ccgcatcaac
     3541 cgcgccaaag gtgaagcgga atccttgcgc cttgttgccg aagccaatgc cgaagccatc
     3601 cgtcaaattg ccgccgccct tcaaacccaa ggcggcgcgg atgcggtcaa tctgaagatt
     3661 gcggaacaat acgtcgctgc gttcaacaat cttgccaaag aaagcaatac gctgattatg
     3721 cccgccaatg ttgccgacat cggcagcctg atttctgccg gtatgaaaat tatcgacagc
     3781 agcaaaaccg ccaaataagc cgtgatgaaa atgccgtctg aagctctgtt ttcggggttc
     3841 agacggcatt tttatttaaa cccgggcaag gttgtattcc gcttcaaa
//
LOCUS       DAWKST010000175         3861 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.175, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000175 DAWKST010000000
VERSION     DAWKST010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.175"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="WJL87_001824"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="WJL87_001824"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ7800226.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLERFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="WJL87_001825"
     CDS             1439..1924
                     /locus_tag="WJL87_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ7800227.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WJL87_001826"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WJL87_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ7800228.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKITGQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVGTLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGTKPKRIKYKPVTR"
BASE COUNT          784 a          944 c         1079 g         1054 t
ORIGIN      
        1 gaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cttcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 ccgtcagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt
      301 ggcgtgcagc gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataag
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgccga tgtcaaacct
      661 gaggacttgg cttggaaggt tttggccgtc aatatttcag atatggcggc gatgggcgcg
      721 ataccgcgtt gggtgttgtt aagtgcggct ttgcccgaat tggatgaggt atggctggaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcattgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgccgaat gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccgt ttgcccgggc ggcgcaggat gtttcagacg gcctcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaggttttag atgccgacgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacga ccgttgtgtt ttttggcttt
     1501 tggtttcggc agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact
     1561 gcctttggca tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg
     1621 tatcgtgctg tttatgtggg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccat ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgt ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgc gttaccggtt tgtatttgat gcgtttcggt ttcgtgcctt cttcgccgag
     2041 tcggcgtttc ttgtcggctt cgtattcctg atagttgccg tcaaagaaca cccatttgga
     2101 gtcgccttcg caagccaaga tatgcgtagc aatacggtcg aggaaccagc ggtcgtgcga
     2161 aatcaccatc acgctgccgg caaattccag caatgcgtct tccaacgcgc gcagggttcc
     2221 cacgtcgagg tcgttggacg gttcgtccag cagcaacaca ttgccaccgc ccaacaaggt
     2281 ttttgccaag tgcaaacgtc cgcgttcgcc gccggaaagc tgccccgtga ttttgctttg
     2341 gtcgctgcct ttgaaattga agcgtcccaa atattggcgg gcggggattt caaactgccc
     2401 gacctgtaaa atatcgcgac cttcggcaat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaagctcat tttcacggtt tgcccgattt tcacttcacc
     2521 agaatcgggc tgctctttgc ccgcaatcat tttaaacagt gtcgatttac ccgcgccgtt
     2581 cggaccgatg atgccgacaa tcgcgcccgc aggcactttg aagctcaaat cgtcaatcag
     2641 cactttgtcg ccgaacgatt tggaaacatt cacaaattca atcacttcgt tacccaaacg
     2701 ctcggcgacg ggaatgaaga tttcctgcgt ttcattgcgt ttttggtatt cgtagttgct
     2761 catttcttca aaacgagcca aacgcgcttt ggacttggct tggcggcctt tggcattttg
     2821 gcgcacccat tccaattcct gcttcatcgc cttcacgcgc gcggcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accatggccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgtgttacc gcaacgactg taccggggaa gcgcacgaga aattgctcca gccactcgac
     3061 agattccgcg tccaagtggt tggtcggctc gtccagcaaa agcatatcgg gtttgctcaa
     3121 caagagtttg cacaaggcaa cgcggcgttt ttcaccgccg gacaaattat cgattttggc
     3181 atcccattcc ggcaggcgca gcgcgtcggc ggcgatttcc aattcgtgtt ccgcaccgcc
     3241 gcccgtggac gaacctgccg caataatcgc ttccaagcgg ccctgctctt ctgccaacgc
     3301 gtcaaaatcc gcatcaggat tggcgtactc ggcatacact tcttccaaac gtttctgcgc
     3361 ggcagccact tcgcccaaac cgctttccac ttcctcgcgc acggtttttt caggatcaag
     3421 ctcaggctct tgcggcaggt agccgatttt aatgccgccc atcggcacgg cttcgccctc
     3481 aaattcctta tccacacccg ccataatccg cagcacggtg gacttgcccg cgccgttcaa
     3541 accgagcagg ccgattttcg cgccggggaa gaaagaaagg gaaatatctt taatgatggt
     3601 tttctgcggc ggcacaacct tgcttacgcg cagcatagaa tagacgtatt gttgggacat
     3661 ggttttctcg ttttcatcaa acaaatttca gacggccatt ttaaccgata atttgattta
     3721 agccagttta tccgcgaacc ggtattgcca aaatcgggca ggattcataa aatccgccta
     3781 tccctttgaa attatacaga caaaaaaaat aataatgata ggagatcgcc gcaccggcaa
     3841 ccatttcgga ttttccaaag c
//
LOCUS       DAWKST010000176         3855 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.176, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000176 DAWKST010000000
VERSION     DAWKST010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.176"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(88..906)
                     /locus_tag="WJL87_001827"
     CDS             complement(88..906)
                     /locus_tag="WJL87_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ7800229.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTGLSDRLPLSVLTGSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPEPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FAPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDISRNFSL"
     gene            complement(973..2223)
                     /locus_tag="WJL87_001828"
     CDS             complement(973..2223)
                     /locus_tag="WJL87_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ7800230.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAVRIWPS
                     HTYTRLTLESTTALKYQHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRNIR
                     AGQNTPDTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDPDDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            complement(2220..2681)
                     /gene="tsaE"
                     /locus_tag="WJL87_001829"
     CDS             complement(2220..2681)
                     /gene="tsaE"
                     /locus_tag="WJL87_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ7800231.1"
                     /translation="MSDFPPVSRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGGGRKCLLTAHTERGRESLPL"
     gene            2871..3683
                     /locus_tag="WJL87_001830"
     CDS             2871..3683
                     /locus_tag="WJL87_001830"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ7800232.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRNNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT          849 a          967 c         1137 g          902 t
ORIGIN      
        1 cactataacc gaaggtaagg gaggggtgtg tccactgtgc cgccgcattt gccgcgtgcg
       61 gaagatacgg ataacaaccc cttggaatta aagggagaag ttgcgggaaa tatccatatc
      121 aggctgggga ccgtacttat ttacggtatt ttcgtatgcc gaaataaaat atttcttgaa
      181 taaaccgtaa tcgccggttt cgtaataggc tacgacagcc tcggcatagg cgggataact
      241 gtcgggggcg aatacgcaag gaaaaaaacc ggagcgcatc agcgacaagg tcatgcagtt
      301 tctggcagta cgtttattgc aatctttaaa atattgaaga tacgccaaat tattatggag
      361 ataaacggcg cggtcgaaag gattttctat tttgggagct tcttgaagca accattttaa
      421 ttccgtatcc aaactttgcg ggttagataa aggggtgtag tctgtcccgc ttatggtaac
      481 gctgtcgcgg cggactacgc cgccggagcc tttttccagt aagttttcgg aaatcaggct
      541 gtgggtggtt tttaagaaat ccaaccagtc aaacggctcg gggctgtcca aaccggacag
      601 caggtgccgg taactttccc tcaggttgat taacattacg gcatccgaat ataacttccc
      661 gcctgcggtt tgcccgagtt taagcaatgc ctgcgtatcg tattgattat aagtgtttcc
      721 ttccaatttg gcagaactat atacaaaatc gataccgatt tcttcaatat tacgcacgga
      781 gcccgttaaa acagataaag gaagccgatc ggacaatccg gtcaggtatt cttgttcgcc
      841 ggctgtaaaa atggggcgga tatttaaaaa atcctgttgg tatctcaatt tatccatacc
      901 tttcatcata aaaacgcggt ttggggcgga agtaggttta tgcctgcctt catccgacta
      961 ccgccttttc aatcaacccc gcttcaatac ggatgtattg atgtagcgtt ggacacccga
     1021 ggcaatggat tgggcgcact gccggcggaa ggattcgctg cccagcaact tctcttcagc
     1081 agggttggac aggaaggcgg tttcgaccag gatagacggc atatcgggtg cgcgcaaaac
     1141 ggcgaaattg gcttcgtcca ccctgccttt gtgcaagtgg ttgagcctgc ccaattcttc
     1201 aagcaccagt ttgccgagtt tgcggctgtc gcgcagcgtg gcggtttggg tcatatcgag
     1261 cagggcggta tcgacgttgc ggttgccgct ggtcggtacg ccgccgaccg cgtcggcatt
     1321 gttttgcgtc tgttccaaga atttggcggc ggagctggtc gcgcctttgg tgttcagcat
     1381 atagaccccc gttccgcgcg cggaggggct ggtgaaagcg tcggcgtgga tggagacgaa
     1441 tacgtccgcc cgccgtgctc gccctttggc gacacgcacg cccaatggga tgaacacgtc
     1501 ttcgttgcgc gtcataaaga cgttgtaacc caaggcttcc aattgttttt tggtttcgcg
     1561 ggcgatggag aggacgacgt gtttttcctg caaaccgccc gggctgacgg cgccggggtc
     1621 ttcgccgccg tgtccggggt cgatcataat gacgggtctg cgcccgtttt tgccgcgttc
     1681 ggtttgtgcg ggagtgtttt gagcgaggtc ggcttcaggg gagccgcgac gggttttgtt
     1741 caggctgccg ttaagcagcg ccatcatcgg atcgtcggga tccatccctt gcggatagag
     1801 atcgacgacg aggcggtttt taaagccgcc gacggggggg agggaaaaaa cttgcgcgta
     1861 ggaaggctgt ttcaaatcga tgacgaggcg gacggtgtcg ggcgtgttct gaccggcgcg
     1921 tatattgcgg ataaaggggt cgtccgccat aaccttctga gacagtccgt gcaatacggt
     1981 attgatgttc gcgttttgta tgtcgacgac caacctgccc gggttgtcga gcgtgaagtg
     2041 ttggtatttg agcgcggttg tgctttccag cgtcaggcgg gtgtaggtgt gcgacggcca
     2101 tatccgtacg gcggtaaact gcggggcgcg taccgttttg gcaacggcgg atgcgatggg
     2161 gcttagggcg aacagtgtgc cggcggtgcg gcggatgatt tgtcttcgtg tcagtttggt
     2221 catagcggca ggctttcgcg tcctcgttcg gtatgggcgg tcagcaggca ttttctgccg
     2281 ccgccgtcgt gtgtcaatgt tgcggtgatg tcggcgggcg gggtaaattc gcctccctgt
     2341 tgcggccatt cgatcaggca gacgctgttt gcggcaaaca gttcgtcaag ccccgcgtct
     2401 tcccattctt cggggaacga gaagcggtag aggtcgaaat ggtgcagggt gaagcgttcc
     2461 agcggataag attcgacgat ggcgtaggtc gggcttttga ctgcgccctg atgacccaat
     2521 ccgcgcagga tgccgcgtgt cagcgtggtt ttgcccgcac ccaaatcccc ttcgagataa
     2581 atgaccagcg gtgcgtttaa acgggaagac cacgccgcac ccaaatcgag tgtggcggct
     2641 tcgtcggcaa ggaatcggga aacgggagga aaatcagaca taggggcttt acaactttta
     2701 tgtggcggat cggaggggca ggcggtcggc aaaccgtgcg cccggcggca gcattgccgt
     2761 acattataac agccaaatcc gcatcctgct ttcagacggc aacggctgtc aagaaaaagc
     2821 ggcgcgtgta caatacgcgg attgtatgtt taggacggat tggaaaaaga atggaaaata
     2881 tcggcaggca gcgacccatc ggcgtttttg actcgggaat cggcggtttg accaatgtgc
     2941 gagcgctgat ggaacggctg ccgatggaga acatcattta tttcggcgac acggcgcgcg
     3001 tgccttacgg gacgaaatct aaggcgacca tcgaaaattt ctcgatgcag attgtcgatt
     3061 ttttattgga acacgatgtc aaggcgatgg ttatcgcgtg caatacgatt gcggcggtgg
     3121 cggggcagaa aatccgtcaa aaaaccggca atatgcccgt tttggacgtg atttccgccg
     3181 gcgcgaaagc cgcgctggca acgacgcgca acaataaaat cggcattatc gccaccaata
     3241 cgacagtcaa cagcaacgcc tatgcgcgcg ccatccacag gaacaacccc gatacgctcg
     3301 tccgcacgca ggccgcgccg ctgctcgtcc ctttggtgga ggaaggttgg ttggaacatg
     3361 aagttacccg cctgaccgta tgcgaatacc tcaaaccatt gcttgcggac ggcatcgata
     3421 cgctggtgtt gggctgcacg cactttccct tgctcaaacc cttaatcggc agggaggcgc
     3481 acaatgtcgc actggtcgat tccgcgatta cgactgccga agaaaccgca cgcgtccttg
     3541 cgcaggaggg attactcaat accgacaaca acaatcccga ctaccgtttt tatgtcagcg
     3601 atattccttt gaaattcaga accatcggcg agcgttttct gggcaggacg atggagcaga
     3661 ttgaaatggt gtctttgggt tagcggcagg tacggacgga aaccgtcccc gtccgcgcca
     3721 tccgcatacg cacccgcaac gccctgccgt attaaacgcc ccgtcgccgc aaatgccgtc
     3781 tgaaaggctt cagacggcat tttttacaca attcccaccg tttcccatcc ttctcgatac
     3841 actgtaatct cgaaa
//
LOCUS       DAWKST010000177         3793 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.177, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000177 DAWKST010000000
VERSION     DAWKST010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.177"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(371..1411)
                     /gene="queA"
                     /locus_tag="WJL87_001831"
     CDS             complement(371..1411)
                     /gene="queA"
                     /locus_tag="WJL87_001831"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ7800233.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPMQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDSHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVEKIE
                     EHKMHSEWFEVPSETVAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVADRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMILGRNEGVVR"
     gene            1528..1785
                     /locus_tag="WJL87_001832"
     CDS             1528..1785
                     /locus_tag="WJL87_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ7800234.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPKHRQPLKEQKWICAN"
     gene            1767..2222
                     /locus_tag="WJL87_001833"
     CDS             1767..2222
                     /locus_tag="WJL87_001833"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ7800235.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKVGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            2334..3695
                     /gene="accC"
                     /locus_tag="WJL87_001834"
     CDS             2334..3695
                     /gene="accC"
                     /locus_tag="WJL87_001834"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ7800236.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIEAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIASGLPLQYKQKDIKIEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFNDPGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
BASE COUNT          935 a         1146 c          912 g          800 t
ORIGIN      
        1 aatttatctt ataaacaaac aaaggcagcc tgaaagattg aatggtctgc acccctaagg
       61 ttggactaac caaccgacta aggtgcagat tattttttgt tgctttttca gcttttcgtt
      121 gggttagata ttcttgccca ctgttttcag gcagccttga atacaaaaaa atggcgtatg
      181 taatatgttt atacgaccaa aacggaatga attttaacgt attgcgcgtc atcaacaatg
      241 actgagtttc tcgcctctcg cgcctgaatc tatgtcatat acagttcctt tcattcaaat
      301 caacaaaaga atgccgtccg aacgtccgtt cagacggcac ttgtcttccc acaatagact
      361 tgaggctgtt ctaacgtacc accccttcgt tccgccccaa aatcatcgca tcgccgtagc
      421 tgaagaaacg gtattcacgt tcgaccgcat aacgatacgc ggcgcggata tgacccatac
      481 ccgaaaacgc gctgacaagc atcagcagcg tcgatttcgg cagatggaaa ttggtaacca
      541 gcctgtcggc aacattaaaa cggtagcccg gcgtgatgaa aatatcggtg tcgccctgcc
      601 ccgctttcag acgacccgtc gcacgcgcgg cagactcgag ggcgcgcata gaagtcgtgc
      661 cgaccgccca gactttgttc ccccgggctt ttgccgcctc aacggcggcg acggtttcag
      721 acggcacttc aaaccattcg ctgtgcattt tgtgttcttc gattttttcc acacgcacgg
      781 gttggaatgt ccccgcaccg acgtgcaggg ttacttctgc ggttactgcg cctttgtctt
      841 tcaggcggcg caaaagttct tccgtaaaat gcagacccgc cgtcggcgcg gcgaccgcac
      901 cctgatattt ggcataaacg gtttgataac ggctgtcgtc atccgcatcg gcggcacgtt
      961 cgatataagg cggcagaggc aaatggccgt tctgttccaa aagttcgtaa acggtttgac
     1021 cgccttcaaa acgcaggcag aacagttcgc cctcacgccc gaccatcacg gcacggatgc
     1081 cgccttcaaa caccagcccc ataccgggct tgggcgattt ggacgaacgg atgtgcgcca
     1141 gtgcggtatg actgtctaaa acacgctcga tcagggcttc gatcctgccg ccgctgtctt
     1201 tttgaccaaa cagccgcgcc ttcattacct tggtgttgtt gaacaccaaa acgtcgcctg
     1261 cctcgacata atcgggcaaa tcgccgaata cccggtcttg catgggcata tccggcagcg
     1321 caaccaaaag gcggctgctg ccgcgcactt cgggcggatg ctgggcaatc agcttttcgg
     1381 gcagggtaaa atcaaaatct gaaatatcca tttttacact ctcgttcggg caagccgcca
     1441 ttatacgcac tttagccctt tttcagacgg catctttgtc cgaaaaacca acagattaga
     1501 ataaacactc ttaacctgga acatcttgtg cgcaaaatca aacttcctgc acatttcccc
     1561 caaaaaccgc cgttttttga tattttactg gacatttacc gacaacttcg ggaaaataaa
     1621 cacattctca cggtcgtttt ccaccacagg aaaaccgtat ccgaacacca ttccgcccgg
     1681 tttgcgccgt tgccgcaagc cggctgtttt ctgaaaaacc aacgcaacaa cccgccgaaa
     1741 caccggcagc ctttaaagga acagaaatgg atttgcgcaa attaaaaaaa ctgattgatt
     1801 tggttgaaga atcgggtatc gccgaaatcg aagtaaccga aggcgaggaa aaagtccgca
     1861 tcacccgaac catcgccgcc gcacccgttt acgccgcgcc cgtacctgcc gccgcgccgg
     1921 ccgtaacgcc tgccgccgca cccgttgcgg catccgcgcc tgccgccgca cctgccgccc
     1981 gcgatttgtc cgacgcgcaa aaatcaccta tggtcggcac gttctaccgc gcacccggcc
     2041 cgaatgccgc gccttttgtc gaagtcggcc aacaggttaa agtcggcgac acgctgtgca
     2101 tcatcgaagc gatgaagctg atgaacgaaa tcgaagccga aaaatccggc acggtcaaag
     2161 aaattttggt cgaaaacggt acgcccgtcg aattcggcga accgctcttc atcatcggat
     2221 aatcctgttt tcagacggca taaacttccg atgccgtctg aaatgctttc ccccttcagc
     2281 gttcccgcac ccttttttac ggacgggttg ccggaaccgc aggaaaggtc atcatgctga
     2341 aaaaagtttt aatcgccaac cgaggcgaaa tcgcattacg cgtactccgt gcctgccgcg
     2401 aaatgggcat tgccaccgtc gccgtgcatt ccgaggccga caaagacagc ctgcacgtca
     2461 aactcgccga cgaatccgtg tgcatcggcc ctgccgcttc cgcgcaaagc tacctcaacg
     2521 tccccgccat tatcgccgcc gccgaagtaa gctgcgcgga cgctgtccac ccgggctacg
     2581 gtttccttgc cgaaaacgcc gatttcgccg aacaggtcga gcagtccggc tttaccttta
     2641 tcggcccgaa acccgacacc atccgcctga tgggcgacaa agtatccgcc aaacacgcca
     2701 tgatcgaagc gggcgtgcct tgtgttcccg gctctgacgg cgcattgccc gacgacggcg
     2761 aagaaatcct caaaatcgcc gataaagtcg gttatcctgt cattatcaaa gcctctggcg
     2821 gcggcggcgg tcgcggtatg cgcgtggtcg agaaaaaaga agacctcctc caatctgtcg
     2881 aaatgaccaa ggcagaagca ggcgcggcat tcggcaaccc gatggtttac atggaacgct
     2941 atttgcaacg tccgcgccac gtcgaaatcc aagtgattgc cgacgaacac ggcaacgccg
     3001 tctaccttgc cgaacgcgac tgttcgctac aacgccgcca ccaaaaagtc atcgaggaag
     3061 caccggctcc gttcatcact gaaaaagaac gcgccaaaat cggcaacgcc tgtgccgacg
     3121 cctgcaaacg catcggctat cgtggcgcag gtacgtttga attcctgtac gaagacggcg
     3181 aatttttctt tatcgagatg aatacgcgcg ttcaggttga gcatccggtt accgagctca
     3241 tcaccggcgt ggacattgtg caggagcaac tccgcatcgc atccggcctg ccgctgcaat
     3301 acaagcagaa agacatcaaa atcgaaggcc acgcgtttga gtgccgtatc aacgccgaag
     3361 acccatacaa cttcatcccc agcccgggcc cgattgaaag ctgccacctg cccggcggct
     3421 tcggtatccg cgtcgacagc cacatttacc aaggctaccg catcccgcct tactacgaca
     3481 gcctgatcgg caaaatctgc gtacacggca aaacgcgtga acaggcaatg gcgaaaatgc
     3541 gcgtcgcact cgccgagctg gcggtaaccg gcatcaaaac caatacgccg cttcaccgcg
     3601 acttgttcaa cgacccaggt ttccaaaaag gcggcgtcag catccactat ttggaacact
     3661 ggctggaaga tcgcaaagcc aaacaggaca aataagccgc cgccgatatg ccgtctgaaa
     3721 cagtttgtcc gttttcagac ggcatttccc ttgccccgcg ccgtctgaaa ccgatttcga
     3781 tatagtggat taa
//
LOCUS       DAWKST010000178         3708 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.178, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000178 DAWKST010000000
VERSION     DAWKST010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.178"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            374..3580
                     /gene="nalP"
                     /locus_tag="WJL87_001835"
     CDS             374..3580
                     /gene="nalP"
                     /locus_tag="WJL87_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
                     /protein_id="HEZ7800237.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGGGGTSAPDFNAG
                     GTGIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPPNLH
                     TGDFTNPNDAYKNLINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGYN
                     ENYKNYTAYMRKEAPEDGGGKDIEASFDDEAVIETEAKPTDIRHVKEIGHIDVVSHII
                     GGRSVDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFGT
                     TSRAGTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNMLF
                     IFSASNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLSA
                     SYEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIGA
                     VGVDSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKGG
                     SQLQLHGNNTYTGKTIIEGGSLVLYGNNKSDMRVETKGALIYNGAASGGSLNSDGIVY
                     LADTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAMTGGKLYMSAGGKGAGY
                     LNRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRGN
                     AARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMPG
                     IRPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLDH
                     NATGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGHSTWS
                     ENSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGTL
                     MQLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSITEGTLVGLA
                     GLKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVAG
                     LGADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT          998 a         1011 c          990 g          709 t
ORIGIN      
        1 cggagccgca gacagtacaa atagtacgga accgattcac ttgttaaaga atcgttctct
       61 ttgagctaag gcgaggcaac gccgtactgg tttttgttca tccactataa ctagggaaat
      121 tcaaattaag ttagaattat ccctatgaga aaaagccgtc taagccggta taaacaaaat
      181 aaactcattg agctatttgt cgaaagttca aatttccatt ttaaaacaat tagtaaaatc
      241 gagtttatcc tagttgtcca agacaacccc tataataata taattcaaaa tataaaaacg
      301 ggttacatct aaacattacg gaatttttat tccctcgcct gaattctatt gtcagattca
      361 aggagacctc atcatgcgaa cgaccccaac cttccctaca aaaactttca aaccggctgc
      421 catggcgtta gctgttgcaa caacactttc tgcctgctta ggcggcggcg gaggcggcac
      481 ttctgcgccc gacttcaatg caggcggtac cggtatcggc agcaacagca gggcaacaac
      541 agcgaaatca gcagcagtat cttacgccgg tatcaagaac gaaatgtgca aagacagaag
      601 catgctctgt gccggtcggg atgacgttgc ggttacagac agggatgcca aaatcaatgc
      661 cccccccccg aatctgcata ccggagactt tacaaaccca aatgacgcat acaagaattt
      721 gatcaacctc aaacctgcaa ttgaagcagg ctatacagga cgcggggtag aggtaggtat
      781 cgtcgacaca ggcgaatccg tcggcagcat atcctttccc gaactgtatg gcagaaaaga
      841 acacggctat aacgaaaatt acaaaaacta tacggcgtat atgcggaagg aagcgcctga
      901 agacggaggc ggtaaagaca ttgaagcttc tttcgacgat gaggccgtta tagagactga
      961 agcaaagccg acggatatcc gccacgtaaa agaaatcgga cacatcgatg tggtctccca
     1021 tattattggc gggcgttccg tggacggcag acctgcaggc ggtattgcgc ccgatgcgac
     1081 gctacacata atgaatacgc atgatggaac caagaacgaa ataatgtctg cagccatccg
     1141 caatgcatgg gtcaagctgg gcgaacgtgg cgtgcgcatc gtcaataaca gttttggaac
     1201 aacatcgagg gcaggcactg ccgaccattt ccaaatagcc aattcggagg agcagtaccg
     1261 ccaagcgttg ctcgcctatt ccggcggtga taaaacagac gagggtatcc gcctgatgca
     1321 acagagcgat tacggcaact tgtcctacca catccgtaat aaaaacatgc ttttcatttt
     1381 ttcggcaagc aatgacgcac aagctcagcc caacacactg accctattgc cattttatga
     1441 aaaagacgct caaaaaggca ttatcacagt tgcaggcgta gaccgcagtg gagaaaagtt
     1501 caatggctcc aaccattgcg gaattactgc catgtggtgc ctatcggcat cctatgaagc
     1561 aagcgtccgt ttcacccgta caaacccgat tcaaattgcc ggaacatcct tttccgcacc
     1621 catcgtaacc ggcacggcgg ctctgctgct gcagaaatac ccgtggatga gcaacgacaa
     1681 cctgcgtacc acgctgctga caacggctca ggacatcggt gcagtcggcg tggacagcaa
     1741 gttcggctgg ggactgctgg atgcgggtaa ggccatgaac ggacccgcgt cctttccgtt
     1801 cggcgacttt accgccgata cgaaaggtac atccgatatt gcctactcct tccgtaacga
     1861 catttcaggc acgggcggcc tgatcaaaaa aggcggcagc caactgcaac tgcacggcaa
     1921 caacacctat acgggcaaaa ccattatcga aggcggttcg ctggtgttgt acggcaacaa
     1981 caaatcggat atgcgcgtcg aaaccaaagg tgcgctgatt tataacgggg cggcatccgg
     2041 cggcagcctg aacagcgacg gcattgtcta tctggcagat accgaccgat ccggcgcaaa
     2101 cgaaaccgtg cacatcaaag gcgatctgca gctgggcggc gaaggtacgc tgtacacacg
     2161 tttgggcaaa ctgctgaaag tggacggtac ggcgatgacc ggcggcaagc tgtacatgtc
     2221 ggcaggcggt aagggggcag gctatctcaa ccgtaccgga caacgtgttc ccttcctgag
     2281 tgccgccaaa atcgggcggg attattcttt cttcacaaac atcgaaaccg acggcggtct
     2341 gctggcttcc ctcgacagcg tcgaaaaaac agcgggcagt gaaggcgaca cgctgtccta
     2401 ttatgtccgt cgcggcaatg cggcacggac tgcttcggca gcggcacatt ccgcgcccgc
     2461 cggtctgaaa cacgccgtag aacagggcgg cagcaatctg gaaaacctga tggtcgaact
     2521 ggatgcctcc gaatcatccg caacacccga gacggttgaa actgcggccg ccgaccgcac
     2581 agatatgccg ggcatccgcc cctacggcgc aactttccgc gcagcggcag ccgtacagca
     2641 tgcgaatgcc gccgacggtg tacgcatctt caacagtctc gccgctaccg tctatgccga
     2701 cagtaccgcc gcccatgccg atatgcaggg acgccggctg aaagccgtat cggacgggtt
     2761 ggaccacaac gctacgggtc tgcgcgtcat cgcgcaaacc caacaggacg gtggaacgtg
     2821 ggaacagggc ggtgttgaag gcaaaatgcg cggcagtacc caaaccgtcg gcattgccgc
     2881 gaaaaccggc gaaaatacga cagcagccgc cacactgggc atgggacaca gcacatggag
     2941 cgaaaacagt gcaaatgcaa aaaccgacag cattagtctg tttgcaggca tacggcacga
     3001 tgcgggcgat atcggctatc tcaaaggcct gttctcctac ggacgctaca aaaacagcat
     3061 cagccgcagc accggtgcgg acgaacatgc ggaaggcagc gtcaacggca cgctgatgca
     3121 gctgggcgca ctgggcggtg tcaacgttcc gtttgccgca acgggagatt tgacggtcga
     3181 aggcggtctg cgctacgacc tgctcaaaca ggatgcattc gccgaaaaag gcagtgcttt
     3241 gggctggagc ggcaacagca tcactgaagg cacactggtc ggactcgcgg gtctgaagct
     3301 gtcgcaaccc ttgagcgata aagccgtcct gtttgcaacg gcgggcgtgg aacgcgacct
     3361 gaacggacgc gactacacgg taacgggcgg ctttaccggc gcgactgcag caaccggcaa
     3421 gacgggggca cgcaatatgc cgcacacccg cctggttgcc ggtctgggcg cggatgtcga
     3481 attcggcaac ggctggaacg gcttggcacg ttacagctac gccggttcca aacagtacgg
     3541 caaccacagc ggacgagtcg gcgtaggcta ccggttctga cggacaggaa gcagacagcc
     3601 gcaaagatca cggtctttgc ggctgtttct tatgaaaaga aaaccctatt ccaattgcct
     3661 gcttctattg tttcaagact tcttccaaag attcggcatc aatcagat
//
LOCUS       DAWKST010000179         3656 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.179, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000179 DAWKST010000000
VERSION     DAWKST010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.179"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            446..1654
                     /locus_tag="WJL87_001836"
     CDS             446..1654
                     /locus_tag="WJL87_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ7800238.1"
                     /translation="MSEAEYFSHFISNGNGKLLEIPQRRGRQDGVFIDWLSFTLHEDS
                     LLKVSGCPLVSDAEYMFVLSKKLEEILGFGITSRCKSKGNKFYDSMFRLGSEEVDYGE
                     VHYGGQRNTVLIELKGVGCNIANPGWELRLKQFLEDSLRPRITRVDLALDFFDGEYTP
                     EQALLDHDNGFFDNSNMRPKSEMVGTAWRREDGSGKTFYVGRKKNSRFVRVYEKGRQL
                     GDKESKWVRFEIQFNHGDMEIPLDILINQGSYFSGAFPICQKFKNMPNPERFDYRKKV
                     ANLTFQHKLRYAKNAVGKLINFMFDMGFDSDEIVRYLKADLGYPKGLEPEKYSLAGLK
                     ESLKFGFIHEQPDVDLEVELEELGIIKFKQSDKFDPDKRLFDPHHDVESERQYQLYLD
                     RMYDLHANQN"
     gene            1675..1986
                     /locus_tag="WJL87_001837"
     CDS             1675..1986
                     /locus_tag="WJL87_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800239.1"
                     /translation="MFNQTQTVTYPATFLGAKKFKGEIDGSNIDTCSVLVATPLPAQS
                     GNAVGFTAAQMKFGDSKNFSKLENLKYPCEVMVTVEMTSTGKGMVPSLIDFQVAEKPK
                     G"
     gene            1990..2190
                     /locus_tag="WJL87_001838"
     CDS             1990..2190
                     /locus_tag="WJL87_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800240.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEIGGGFQIFQFFVKSE"
     gene            2417..2647
                     /locus_tag="WJL87_001839"
     CDS             2417..2647
                     /locus_tag="WJL87_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800241.1"
                     /translation="MNIVKKYAVKAALAAGIFTPAIVMADTFDASAIGTQVANVIMGF
                     VSMVSAVGMAAITVILAIQGFKMAWSMIKSVR"
     gene            2675..2974
                     /locus_tag="WJL87_001840"
     CDS             2675..2974
                     /locus_tag="WJL87_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800242.1"
                     /translation="MGYRVGINCFDTRLQADDYLLSSLPPTVTQDGKIIRPERVGEKW
                     VLNGKPVMLSYPKCSNFEQIKQGSYVGSTVLILFVVIYGFRLLINFLKDIGKVGA"
     gene            3093..3413
                     /locus_tag="WJL87_001841"
     CDS             3093..3413
                     /locus_tag="WJL87_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ7800243.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMALIASKEFEEFVRNVKE
                     YDVIECFSYFLNKRVTHNIWKIYFSDESNIFIRKSEENGKISHEFIYSEFSDSNTDFN
                     VLFS"
     gene            3397..>3656
                     /locus_tag="WJL87_001842"
     CDS             3397..>3656
                     /locus_tag="WJL87_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ7800244.1"
                     /translation="MCCSLSFAEPARIDDRIIKFRPSKSKFFESPGYRKINNEFSKFT
                     EAANVEHIPTGAKARINAKITASVSRAGVLAGVGKLARLGAK"
BASE COUNT         1125 a          622 c          838 g         1071 t
ORIGIN      
        1 atatgatgcg ctctatcaaa ggggcgcatt acttttctta acattcccct ttgacagcca
       61 agtgaaaggg gcttttttat gtcagtagca aatgtaatat tttcttgttc ctattggaga
      121 atatttaaaa aatcagatta ttgcgtttta tgtttctatc agttcaggca tggtgaaccg
      181 cataaactcg ctgaacaaga aaatttttca aagctttatc aggcgttcga ttatatagat
      241 tcggttggct cgaattttcc ggtaattatc acaacagacg gttgtggtct ttcttcttga
      301 tctttaacag tttgtcagga ttgggctttc ggtcgttgac cgttggacgc gctttagcgc
      361 ggcagacggg aaacggctga aagccccccc cctgactaac agggggggag cgaaataaaa
      421 caaatcccta aaggtactga acaaaatgag tgaagcagaa tatttttccc atttcatatc
      481 taacggcaat gggaagttat tagaaattcc gcaacgtagg ggcaggcagg acggcgtttt
      541 tatagattgg ttgtcattca cactgcacga agattccttg ctgaaagttt ccggatgccc
      601 cttagtttcc gatgccgaat atatgtttgt tttaagcaaa aaattggagg aaatattagg
      661 gtttggcatc acgagcagat gcaaatcgaa gggcaataaa ttttacgatt cgatgtttag
      721 gttgggatcg gaagaagttg actacggcga agtccattac ggaggtcagc gaaatacggt
      781 tttaatcgaa ttgaaaggtg taggttgcaa cattgcaaat ccaggttggg aattgaggct
      841 taagcagttt ttggaagatt cattgaggcc gaggataacg cgggtagatt tggcacttga
      901 tttttttgat ggggagtaca cgccggaaca ggcacttttg gatcacgata acggtttttt
      961 cgataacagt aacatgaggc cgaaatctga aatggttgga acggcttggc ggagagagga
     1021 cgggagcggc aagacatttt atgtaggtcg caagaaaaat tctcgttttg tgcgtgttta
     1081 tgagaaaggc aggcagctag gcgataaaga aagcaagtgg gtaaggttcg aaattcagtt
     1141 taatcatgga gatatggaaa tacctttgga tattctgata aatcaaggtt cttacttttc
     1201 aggcgctttc ccgatttgtc agaaatttaa aaatatgccg aatccggaaa ggttcgatta
     1261 ccgtaaaaaa gtggctaatt taacttttca gcataaattg agatacgcaa aaaacgcggt
     1321 cggcaaactg attaatttca tgtttgatat gggttttgat agtgatgaaa ttgtcagata
     1381 tctgaaggca gatttggggt atcccaaagg gctagaacct gaaaaatatt cgttggccgg
     1441 attgaaggaa tctttgaaat tcggctttat ccacgaacaa ccggatgtag atttagaggt
     1501 tgaattggaa gaactcggaa ttatcaaatt taagcaatca gataaattcg atccggataa
     1561 aaggcttttc gatccacatc acgatgtaga aagtgagagg caatatcagc tttatctcga
     1621 cagaatgtat gatcttcatg caaatcaaaa ttaacctaaa aaggaaaaat taatatgttt
     1681 aatcaaactc aaactgtaac ttatcccgca acttttttag gagctaaaaa attcaaaggc
     1741 gaaattgatg gctctaatat cgacacttgt tccgtattgg ttgcaacacc tttgccggca
     1801 cagtcgggaa atgctgttgg attcacggca gcacaaatga agttcgggga cagtaagaat
     1861 ttctcaaaat tagagaatct caaatacccg tgcgaagtta tggtaacggt tgaaatgact
     1921 tcgacaggta agggcatggt tccttcatta attgattttc aggtggcaga aaagccgaaa
     1981 ggttgattta tgaaatttga agaacgtttc atagttcaag acttggaaac gcatgacttt
     2041 atttatcccg atcctttcgg tgatgtgggg tttactcaaa atattaaatc agcaggtcaa
     2101 tttgaaagct acgaagatgc gttgaattca ggcataaatg aaataggcgg aggattccag
     2161 atatttcagt tcttcgtaaa atcggaataa aagaaaaaca ggctcggcgg gcggtctgtc
     2221 aacctttcac aaagcccgca aaaaggatta aaaatgaata aagcaacttt aattatcgga
     2281 ttttttgttt gcctcattct tttcattttc tcaattctct atttcataaa ttaaaaacaa
     2341 aattcgggag caggcttcgc ccggatttaa acctaagaag ccgtattact ttaaatatca
     2401 aaaaaggaaa aaaacgatga acatcgttaa aaaatacgct gtaaaagcag cattggcagc
     2461 cggtatcttc acaccggcca ttgttatggc agataccttt gatgcatccg cgattggtac
     2521 gcaagtagcg aatgtaatca tgggtttcgt gtcaatggtt tccgccgtgg gtatggcggc
     2581 cattaccgtg attcttgcaa tccaaggctt caaaatggct tggagcatga ttaaatctgt
     2641 cagataaaca gagtgaagaa aaaggggcgt ataaatgggc tatcgtgtcg gcataaattg
     2701 ttttgataca agattgcagg cagacgacta tttattgtcg tcccttcctc ctactgttac
     2761 ccaggacgga aaaatcatca ggccggaaag ggtgggcgaa aaatgggtct tgaacggaaa
     2821 gccggtaatg ttgtcctatc cgaaatgttc caattttgag cagatcaaac agggttctta
     2881 tgtcggttcg acggttttaa ttctgtttgt cgttatttac ggcttcaggc tgctgattaa
     2941 tttcttaaaa gacataggca aagtaggggc gtgatgatgt ttgtcgattt ttggtttttg
     3001 ctcggatttt tcctggcttt gtctgtcgct ttgatattta tatgacgtgt tttaaaatca
     3061 ggctttcaaa acaacctttg aaagacagaa acatgaacaa gccgtttatc acgcaggcgc
     3121 agttggcact ttataaatat cagccgtcca gcaagtattt tgggcagtcg atggcattaa
     3181 ttgcgtctaa ggaatttgaa gagtttgtaa gaaatgtaaa agaatacgac gtaatagaat
     3241 gtttctctta ttttttaaat aagagggtaa cgcataatat ttggaaaatt tatttttctg
     3301 atgagtctaa tatttttatt aggaagtcag aagaaaatgg aaaaatttcg catgaattta
     3361 tttactcgga attttctgat agcaacaccg attttaatgt gttgttctct tagttttgca
     3421 gaaccagcaa gaatagatga tcgaataata aaatttaggc catctaaatc aaagtttttt
     3481 gaatctccag gatatagaaa aatcaataat gaattttcta aattcacaga agcggcaaat
     3541 gtcgaacata tccccacggg cgcaaaagcc cgaatcaacg ccaaaataac cgccagcgtt
     3601 tcccgcgctg gcgtattggc gggggtcggc aagcttgccc gcttaggcgc gaaatt
//
LOCUS       DAWKST010000180         3448 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.180, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000180 DAWKST010000000
VERSION     DAWKST010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.180"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            43..720
                     /locus_tag="WJL87_001843"
     CDS             43..720
                     /locus_tag="WJL87_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEZ7800245.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKDGTPNNKCDSGKKGQGMLAFGDKNGNK
                     TYDGDTADVLLRSVVLNDDINNKRINYAFNHIAFGQTQPTADRVVWTFNQNGTFGYTK
                     DQHLTSKSSFFYSDGYIQIVLTDARAVSDADKKFRSAVVLINSSGRVEVCPRGDRRTR
                     RTMCQYK"
     gene            744..1364
                     /gene="pilV"
                     /locus_tag="WJL87_001844"
     CDS             744..1364
                     /gene="pilV"
                     /locus_tag="WJL87_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEZ7800246.1"
                     /translation="MKNNDCFRLKDSQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNHTLPHTLSAVDGDFA
                     IDAMKTKGQLAEAQLKRFSYELKNALPDAAAIHYTVCKDSSGNAPTLSGSTFSQNCDG
                     SANGDTLIKVLWVNDSAGDSDIARTNLETNGDNIVYTYQARVGGRE"
     gene            1361..2299
                     /locus_tag="WJL87_001845"
     CDS             1361..2299
                     /locus_tag="WJL87_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEZ7800247.1"
                     /translation="MRRKMLNVPKGNYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLSEQQDLRNAATLIVRDARMAGSFGCFNMSEHPAIDVISDTTQQNSP
                     FSLKRNGIDKLIPIAESSNINYQNFFQFGSALIFQYGIDDVNASTATTVVSSCAVISK
                     PGKQIPTLEDAKKELKIPQDKEQNGNIARQRHVVNAYAVGRIAGEEGLFRFQLDDKGK
                     WGNPQLLVKKVRRMKVRYIYVFGCPEDDDAGKEETFKYTDKFDSSTNAVTPAGVEVLL
                     SSGTDTKIAASSDNYIYAYRIDATIRGGNVCANRTL"
     gene            2278..2877
                     /locus_tag="WJL87_001846"
     CDS             2278..2877
                     /locus_tag="WJL87_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ7800248.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCEKGLCTAVNMRT
                     NTNNGNEEAFDNIVVQGKPTVEAVKRSCPAKSTGLCIDNKGMEYKKGTRSVSKMPRYI
                     IEYLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            2882..3355
                     /locus_tag="WJL87_001847"
     CDS             2882..3355
                     /locus_tag="WJL87_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ7800249.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNTVKQLILKNPLDDNQTIKSKLEIFVSGYKMNPKIAKKYSVSVRFVDKEKSRA
                     YSLVGVPKAGTGYTLSVWMNSVGDGYKCRDAASARAYSETLSADAGCEAFSNRKK"
BASE COUNT          988 a          686 c          874 g          900 t
ORIGIN      
        1 aatttaatcc actatacatt accagttaac gttctattgc ttatgtgtac acgaaaacaa
       61 caaggtttca cgctaacaga gctgctcatc gtgatggtca ttgcagccat tatggcgatg
      121 atagccctcc ccaatatgag ccaatggatt gcatcccgcc gcattgccag tcacgcggag
      181 cggattgcca accttttgcg tttctccagg ggcgaagccg tccggctcaa tctccctgtc
      241 tatatctgtc ctgttcaagt taaaaaagac ggtacgccca acaataaatg tgactccggc
      301 aagaaggggc agggaatgtt ggcttttggc gataagaatg gcaataagac atatgacggc
      361 gatacggcgg atgttcttct ccgcagtgtg gtattgaatg atgatatcaa taataagcgg
      421 attaattatg ccttcaacca tatcgctttc ggtcagactc agccgaccgc tgaccgtgta
      481 gtttggacat tcaatcaaaa cgggacgttc ggttatacga aagaccagca tcttacaagc
      541 aaatccagct ttttttattc cgacggttat atccaaatcg tgctgacaga tgcgagagca
      601 gtttcagatg ccgataagaa attccgttcg gcggtggttt tgattaacag cagcggcagg
      661 gtcgaagttt gccctagggg tgatcggcgt actcggcgta ctatgtgcca gtataaataa
      721 cagtttcagt tttaaaaatg aatatgaaga ataatgattg cttccgcctg aaagattccc
      781 agtccggtat ggcgctgata gaagtcttgg tcgctatgct cgttctgacc atcggtattt
      841 tggcactatt gtctgttcag ttgcggacag tcgcttccgt cagggaggca gagacgcaaa
      901 ccatcgtcag ccaaatcacg caaaacctga tggaaggaat gttgatgaat ccgaccattg
      961 attcggacag caacaagaaa aactataatc tttacatggg aaaccataca ctaccgcata
     1021 cactatcagc tgtggatggc gattttgcga ttgatgccat gaaaactaag gggcaattgg
     1081 cagaggcaca attgaagaga tttagttatg agctgaaaaa tgccttgccg gatgcggcag
     1141 ccatccatta caccgtctgc aaggattcgt cgggtaatgc gccgacattg tccggcagta
     1201 ctttttctca aaattgcgat ggtagtgcaa atggggatac tttgattaaa gtattgtggg
     1261 taaatgattc ggcaggggat tcggatatcg cccgtacgaa tcttgagacg aacggcgaca
     1321 atatcgtata tacctatcag gcaagggtcg gaggtcggga atgagacgta aaatgctaaa
     1381 cgtaccaaaa ggcaattatg atggtatgaa gggttttacc attattgaat ttttggttgc
     1441 gggcatgctc agtatgattg tcctgatggc ggtcggatcg agttacttca catcccggaa
     1501 attaaatgat gcggcaaacg agcgtctttc cgagcaacag gatttgcgga atgcggcaac
     1561 attgattgtc cgcgatgcaa gaatggcggg gagcttcggt tgtttcaata tgtccgagca
     1621 tcctgcaatt gatgttattt ccgatacgac gcaacaaaat tctccttttt ccttaaaaag
     1681 gaacggtata gataaactta ttcccatagc ggaatcttca aatatcaatt atcagaattt
     1741 tttccagttt ggtagcgcat tgatttttca atacggaatc gatgatgtta atgcaagcac
     1801 cgcgactacc gtcgtcagca gctgtgccgt aatatcgaaa ccgggcaagc aaatccctac
     1861 tttagaagat gcaaaaaaag aattgaagat tccgcaggat aaggagcaaa atggcaatat
     1921 agcgcgtcaa aggcatgtgg tcaatgccta tgcggtcggc aggattgccg gtgaggaagg
     1981 tttgttccgc ttccaattgg atgataaggg caagtggggt aatcctcagt tgctcgtgaa
     2041 aaaggttaga cgtatgaaag tgcggtatat ctatgttttc ggctgtcctg aagatgacga
     2101 tgccggcaaa gaggaaacat tcaaatatac ggataaattc gacagctcca caaatgctgt
     2161 tacgcccgcc ggggtggagg ttttattgag tagcggtact gataccaaga ttgccgcttc
     2221 ttcagacaat tatatttatg cttaccgtat cgatgcgaca atacgcgggg gaaatgtatg
     2281 cgcaaacaga acactttgac gggaatcccg acttctgacg gacagagggg gtttgcactg
     2341 tttatcgtgc tgatggtgat gatcgtcgtg gcttttttgg ttgtaactgc cgcgcagtct
     2401 tacaataccg agcagcggat cagtgccaac gaatcagaca ggaaattggc tttgtctttg
     2461 gccgaggcgg ctttgcggga aggcgaactt caggttttgg atttggaata tgatacggac
     2521 agtaaggtta catttagcga aaactgtgaa aaaggtctgt gtaccgcagt gaatatgcgg
     2581 acaaatacaa ataatggtaa tgaagaggct tttgacaata tcgtggtgca aggcaagccc
     2641 accgttgagg cggtgaagcg ttcttgccct gcaaaatcta caggcctgtg cattgacaat
     2701 aaagggatgg aatataagaa aggcacgaga agcgtcagca aaatgccacg ttatattatc
     2761 gaatatttgg gcgtgaagaa cggagaaaat gtttatcggg ttactgccaa ggcttggggt
     2821 aagaatgcca ataccgtggt cgtccttcaa tcttatgtaa gcaataatga tgagtaataa
     2881 aatggaacaa aaagggttta cattgattga gatgatgata gtcgtcgcga tactcggcat
     2941 catcagcgtc attgccatac cttcttatca aagttatatt gaaaaaggct atcagtccca
     3001 gctttatacg gagatggtcg gtatcaacaa tactgtcaaa cagcttattt tgaaaaatcc
     3061 cctggacgat aatcagacca tcaagagcaa actggaaata tttgtctcag gctataagat
     3121 gaatccgaaa attgccaaaa aatatagtgt ttcggtaagg tttgtcgata aggaaaaatc
     3181 aagggcatac agcttggtcg gcgttccgaa ggcggggacg ggttatactt tgtcggtatg
     3241 gatgaacagc gtgggcgacg gatacaaatg ccgtgatgcc gcttctgccc gagcctattc
     3301 ggagacttta tccgcagatg ccggctgcga agctttctct aatcgtaaaa aatagggctg
     3361 ttttgccaat gccgtctgaa gattaatttt cagacggcat ttttatgggt atagtggatt
     3421 aacaaaaatc aggacaaggc gacgaagc
//
LOCUS       DAWKST010000181         3427 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.181, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000181 DAWKST010000000
VERSION     DAWKST010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.181"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            314..3079
                     /locus_tag="WJL87_001848"
     CDS             314..3079
                     /locus_tag="WJL87_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ7800250.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQQYFVQTGGLKFNSNSGKWER
                     DLQRQFWKTSYYKQYKDPQKLKKYIEDQDKDWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNSYTNLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYSSEGEFKRAFGENSPTYKKHCNPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNFRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADGLCNGKYGGTSKSVLTNFARGRTFL
                     MTMSYKF"
     gene            3122..3364
                     /locus_tag="WJL87_001849"
     CDS             3122..3364
                     /locus_tag="WJL87_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800251.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPQKRESRHSMLRQFIEND"
BASE COUNT          974 a          846 c          828 g          779 t
ORIGIN      
        1 gcctgcgcgg gaatgacggc tgcagatgcc cgacggtctt tatagcggat taataaaaat
       61 caggacaagg cggcgagcca cagacagtac aaacagtacg gaaccgattc acttggtgct
      121 tcagcacctt agagaatcgt tctctttgag ctaaagcgag acaacgctgt actggttttt
      181 gttaatccac tataaatatc caattgaaat cttcagacgg tatatcaaat ttacactttt
      241 ttaatgttta tgccgcctga aaaaaatgct agtatatttc ctaattgtct gactgtttat
      301 tgttgaggaa aatatgagat cttctttccg gttgaagccg atttgttttt accttatggg
      361 tgttatgcta tatcatcata gttatgccga agatgcaggg cgcgcgggca gcgaggcgca
      421 gatacaggtt ttggaagatg tgcacgtcaa ggcgaagcgc gtaccgaaag acaaaaaagt
      481 gtttaccgat gcgcgtgccg tatcgacccg tcaggatata ttcaaatcca gcgaaaacct
      541 cgacaacatc gtacgcagca tccccggtgc gtttacacag caagataaaa gctcgggcat
      601 tgtgtctttg aatattcgcg gcgacagcgg gttcgggcgg gtcaatacga tggtggacgg
      661 catcacgcag accttttatt cgacttctac cgatgcgggc agggcaggcg gttcatctca
      721 attcggtgca tctgtcgaca gcaattttat tgccggactg gatgtcgtca aaggcagctt
      781 cagcggctcg gcaggcatca acagccttgc cggttcggcg aatctgcgga ctttaggcgt
      841 ggatgacgtc gttcagggca ataataccta cggcctgctg ctaaaaggtc tgaccggcac
      901 caattcaacc aaaggtaatg cgatggcggc gataggtgcg cgcaaatggc tggaaagcgg
      961 agcatctgtc ggtgtgcttt acgggcacag caggcgcagc gtggcgcaaa attaccgcgt
     1021 gggcggcggc gggcagcaca tcggaaattt tggcgcggaa tatctggaac ggcgcaagca
     1081 gcaatatttt gtacaaacag gcgggttgaa attcaattcc aacagcggaa aatgggaacg
     1141 cgatttgcaa aggcaattct ggaaaaccag ttactataaa caatacaaag acccccagaa
     1201 actgaaaaaa tacatcgaag accaagataa agactggcgg gaaaacctgg cgccgcaata
     1261 cgacatcacc cccatcgatc cgtccagcct gaagcagcag tcggcaggca atctgtttaa
     1321 attggaatac gacggcgtat tcaataaata cacggcgcaa tttcgcgatt taaacaccaa
     1381 aatcggcagc cgcaaaatca tcaaccgcaa ttatcaattc aattacggtt tgtctttgaa
     1441 ctcgtatacc aacctcaatc tgaccgcagc ctacaattcg ggcaggcaga aatatccgaa
     1501 agggtcgaag tttacaggct gggggctttt aaaggatttt gaaacctaca acaacgcaaa
     1561 aatcctcgac ctcaacaaca ccgccacctt ccggctgccc cgcgaaaccg agttgcaaac
     1621 cactttgggc ttcaattatt tccacaacga atacggcaaa aaccgctttc ctgaagaatt
     1681 ggggctgttt ttcgacggtc ctgatcagga caacgggctt tattcctatt tggggcggtt
     1741 taagggcgat aaagggctgc tgccccaaaa atcaaccatt gtccaaccgg ccggcagcca
     1801 atatttcaac acgttctact tcgatgccgc gctcaaaaaa gacatttacc gcttaaacta
     1861 cagcaccaat accgtcggct accgtttcgg cggcgaatat acgggctatt acagctcgga
     1921 aggcgaattt aagcgggcat tcggagaaaa ctcgccgaca tacaagaaac attgcaaccc
     1981 gagctgcggg ctttatgaac ccgtattgaa aaaatacggc aaaaagcgcg ccaacaacca
     2041 ttcggtcagc attagtgcgg acttcggcga ttatttcatg ccgttcgcca gctattcgcg
     2101 cacacaccgt atgcccaaca tccaagaaat gtatttttcc caaatcggcg actccggcgt
     2161 tcacaccgcc ttaaaaccag agcgcgcaaa cacttggcaa tttggcttca atacctataa
     2221 aaaaggattg ttaaaacaag atgatacatt aggattaaaa ctggtcggct accgcagccg
     2281 catcgacaac tacatccaca acgtttacgg gaaatggtgg gatttgaacg gggatattcc
     2341 gagctgggtc agcagcaccg ggcttgccta caccatccaa catcgcaatt tcaaagacaa
     2401 agtgcacaaa cacggttttg agttggagct gaattacgat tatgggcgtt ttttcaccaa
     2461 cctttcttac gcctatcaaa aaagcacgca accgaccaac ttcagcgatg cgagcgaatc
     2521 gcccaacaat gcgtccaaag aagaccaact caaacaaggt tatgggttga gcagggtttc
     2581 cgccctgccg cgagattacg gacgtttgga agtcggtacg cgctggttgg gcaacaaact
     2641 gactttgggc ggcgcgatgc gctatttcgg caagagcatc cgcgcgacgg ctgaagaacg
     2701 ctatatcgac ggcaccaacg ggggaaatac cagcaatttc cggcaactgg gcaagcgttc
     2761 catcaaacaa accgaaactc ttgcccgcca gcctttgatt tttgattttt acgccgctta
     2821 cgagccgaag aaaaacctta ttttccgcgc cgaagtcaaa aatctgttcg acaggcgtta
     2881 tatcgatccg ctcgatgcgg gcaatgatgc ggcaacgcag cgttattaca gctcgttcga
     2941 cccgaaagac aaggacgaag acgtaacgtg taatgctgat ggcttgtgca acggcaaata
     3001 cggcggcaca agcaaaagcg tattgaccaa ttttgcacgc ggacgcacct ttttgatgac
     3061 gatgagctac aagttttaaa ggcagcccgc gttttgtgga aaaccgcaaa aaaaagccaa
     3121 aatgccgtct gaaagccctt cagacggcat ttgtttcccc aaacgcatca tcctgccgca
     3181 agcctatgcc aatccgtttt atcgcatcgg caactcaaag aaaaatccat ttcattccca
     3241 cgcagggaag ccggtttttg atttcggtta tttttggttg tttcgggtaa tttatgagtc
     3301 gtcattcccg caaaagcggg aatctagaca ttcaatgcta aggcaattta tcgaaaatga
     3361 ctgaaactca aaaaactgga ttcccacttt cgtgggaatg acgcggtgca ggtttccgta
     3421 tggatgg
//
LOCUS       DAWKST010000182         3378 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.182, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000182 DAWKST010000000
VERSION     DAWKST010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.182"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(214..1278)
                     /locus_tag="WJL87_001850"
     CDS             complement(214..1278)
                     /locus_tag="WJL87_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ7800252.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLCALTRFIRENRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIIH
                     NGIDTDRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTENIASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIVEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAAH
                     QCVAARFTINHTADKLLDAI"
     gene            complement(1279..2037)
                     /locus_tag="WJL87_001851"
     CDS             complement(1279..2037)
                     /locus_tag="WJL87_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ7800253.1"
                     /translation="MKKVSVLIVAKNEANHIRECIESCRFDKEVIVIDDHSTDNTAEI
                     AEGLGAKVFQRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            complement(2112..2187)
                     /locus_tag="WJL87_001852"
     tRNA            complement(2112..2187)
                     /locus_tag="WJL87_001852"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2152..2154),aa:Ile,seq:cat)
     gene            complement(2241..3378)
                     /locus_tag="WJL87_001853"
                     /pseudo
     CDS             complement(2241..3378)
                     /locus_tag="WJL87_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
BASE COUNT          765 a          822 c          899 g          892 t
ORIGIN      
        1 tctactatac attgtaacaa agcacttgat gcggcagcac aagccgggga agacgctgca
       61 aaactttgcc aataggctgc gtaatatcgg gggatattgg gattttctta tttttaagga
      121 tggattcggg gtgtttcttt ccaatgaacg aatataattt ggcggtttgt tgcatcaaag
      181 gctttcagac ggcatatgcc gtctgaaagc agtttatatt gcatccaata atttgtcggc
      241 ggtatggttg atggtaaaac gggcggcgac gcattgatga gctgccgttg ccagtgcgtt
      301 ttttgcttcg gggtttaaga cgaggcgttc gatttcgtcc gccaaagatt caggcgtaag
      361 cctatccagc agaatccccg attgatgatg ttcgacaatt tccttttgcg cccccaaagt
      421 attggaaatc accgccgttc ggcagtacat cgcctcgcat aatgacaaac cgaatgcctc
      481 cgggacgagg ctgggcaaaa ccacgacatc gctttggcgg taaaacgaag caatgttttc
      541 ggtaaaccct tcaaaagaaa caaatggttc tgctcctgaa gcagatacgt cccgcttcag
      601 gcggcacata taatcaggat gtccgtgccc tgccaatttg agtctgattt gaggatattt
      661 ccgatgcaat atcacacagg cttcaattag gttttccaat cctttttccg gactgatcct
      721 gccggcgtag gcgacggtaa aaaaacggct gtcgggtttt tcttgagagg gagggaaccg
      781 atcggtatcg ataccgttat gaataatccg gtatttttct ttaaagggat tgtcggcggt
      841 ttgcacatcg taaaccagac gggaaacgca aataaagcgg tctgtgtttt tctgtatcag
      901 gcggtggtaa aaatcggttt tgttggcgac gacattatgt ttgacaaata tcaggcgcac
      961 cccggtcagt tttttcaaaa gtatggataa ggcggcaatt ttgccggtgt gtatcatcag
     1021 gtgggaaatg cggttttccc ggataaagcg ggtaagcgcg cagagcgaaa acagcccgtt
     1081 gagcgtgtga aggcgtgtcg tgaaaacgga agaaacttcg gaaaagcgtc tgtgcatcaa
     1141 ctcattattt ttattgacgg cggtaaacat tgtgcagccc cgaagcccca atgcttttga
     1201 aacatcatag acatattgtt cgcccccacc ccaaatcttg gccgatacga tatttaatat
     1261 cctgaattct ttttccattt aaaattttcc gccagatttg tacagataat atagtttgac
     1321 atatttaatc atcgtgtaat agctgtggtt gacggacata atccaaccca tttttccatc
     1381 aagaaaccct ttgttcagaa tataaatttt gaaaaaaccc caaatcggac ggaggataat
     1441 gtccctaacg aaacgcacgg gctttccctg ctctcggtat ttctcggctg agatggaagt
     1501 atatttgttg aacttgttga aatattgttc ccagttgtcg tacgtataat ggtacataaa
     1561 atgtttcaga cggcgtttgg ggtagggggt ttgtacggtt tcgtgtactt tgccttgcac
     1621 cgaactgtct ttcttcggca tcaaacggca tacgctgtcg ggacgcatcg cgccgtgtgt
     1681 ggcgggatgg ttggggaaaa ggttgcggcg ctcgacaaaa taggcggcat aatcgccggt
     1741 ttggacaatt tttgagattt catcagatag ttccggcgtg cagcgttcgt ctgcatcaat
     1801 caggaaaacc cattctccgc ctgcctgttc gatggcaaat gttttttgcg ctccgaaatc
     1861 cccgttcaaa tgccgttgga agacttttgc acccaaaccc tcggcaattt cggcagtatt
     1921 gtcggtgctg tggtcgtcga taacgataac ttctttatcg aaacggcaac tttcaataca
     1981 ttcccgaatg tggtttgctt cgtttttggc aacaatcaat acgctgactt ttttcattat
     2041 caaggtttct caaagcattt tggtttcaca gggcggcaaa aaagcccaaa catacgtccg
     2101 ggcttgggaa ttggtgggtc cggtggggtt cgaacccacg accaagggat tatgagtccc
     2161 ctgctctaac ccctgagcta cagacccgtc aagagtaggc gcgtattgta tagcatagcg
     2221 gtgggattga caaacttatt tcatccgatg attaagacgc agttgcttcc cccgaaggca
     2281 aacgatgagc tggcggcaat gcgtttttcg gtttcccagc ggctgccgct gccggtcagg
     2341 ttgatggcgg gcaggttggg gtcgttctgc ccgtcccaaa gccggggcgg aagttttcct
     2401 tcgggattgc tttgccggtt ggcaatgccc cacgcgaacg cggcttcgat tgcgtccgcc
     2461 gcgcccagcg tgtgtccggt ttgcggcttg gtggacgtgc agggcgtatt gttgccgaaa
     2521 accgctgcaa cggcgcggct ttccatattg tcgttgtggt gcgtcccggt gccgtgcaga
     2581 ttaatccagc cgatgtcttc gggcgcaaga cctgcgtgtt gcaatgccgt ctgaaaggca
     2641 aggattgcgc cttacgcgtc ggggcggggc gtggaaatat ggtaggcatc gctgttggct
     2701 ccgtagccga cgagttcggc gtaaattttc gcaccgcgcc gtttggcgtg ttccaattct
     2761 tccagcacga aaatccccgc gccttcgccg atgaccagcc cgtcgcggtt cgcgtcgtat
     2821 gggcgcgggg ttttttccgg ttcgccgttg cggcggctgg cggcataaag cgagtcgaaa
     2881 acatacactt cggacgggaa aaattcttcg cctccgcccg ccagcatcat atcggtcaga
     2941 ccgtatttga tggcttcgta ggcataacct atgccttggc tgccggacga acacgcgctc
     3001 gatgtcggga tgatgcgtcc tttcagcccg aaaaagatgc cgatattggc ggcggcggtg
     3061 tgcggcatca tacgcacata ggtgttggca ctgaagttgc gcgacatgcc ggtcagcaac
     3121 aattcgccta catcgccgat gtctttggtg ctgccgctgg aagagccgca ggcaacgccc
     3181 atgcgtccgt cagtaatgct ttcgtctccg agcaaaccgg catccgtcaa agcctgctcc
     3241 gccgcatcga cgcacaaata cgacacacgc cccatacttc tgagctgctt gcgcgtccaa
     3301 tgtttcggcg gcgcgtaatt ttcaatcggc gcacccagtt gcgcttccaa ttcggggaaa
     3361 cgttcgtgcc aatccact
//
LOCUS       DAWKST010000183         3355 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.183, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000183 DAWKST010000000
VERSION     DAWKST010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.183"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            44..811
                     /locus_tag="WJL87_001854"
     CDS             44..811
                     /locus_tag="WJL87_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ7800254.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEQTSFNNLEPMTGFE
                     HTVTFDFQGIKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINLIEISVYYKKTDQGW
                     VLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERLLTYGVKKMPSAYPE
                     YEAYEDKRHIPENPYFHEFYYIKKGENPAIITHRNYHRYGENDYSTSVGSCINGFTVR
                     YYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNSSKK"
     gene            828..1103
                     /locus_tag="WJL87_001855"
     CDS             828..1103
                     /locus_tag="WJL87_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800255.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKTRLMCISVFWIVVFNL"
     gene            complement(1190..2323)
                     /locus_tag="WJL87_001856"
     CDS             complement(1190..2323)
                     /locus_tag="WJL87_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800256.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEVPE
                     NPNAFVAKLARLFRNADRAVVIVKESMRTEESLAETVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGEEVWLDYYIGEGGLVAVSLLQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHRENYETTGEYRVVWQPDGSVFDAAGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDYVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHAQQTWYLDGGRI
                     IREEKQGDRLPDFPLNLENLEKEVRRYAEAAARRSGGRRDLSH"
     gene            complement(2443..2937)
                     /locus_tag="WJL87_001857"
     CDS             complement(2443..2937)
                     /locus_tag="WJL87_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800257.1"
                     /translation="MENRFKKIVKDILSFAQFVSEAENKIPNQSSRYYDCWWELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKHLFAEEVPENNIVCDISHFLHFADWA
                     EADIPNQSRLYAEYWWEMEMFKMSVLEDWERSGKPLDWKKWQRQYRSEAFELVKKLNR
                     SKQE"
     gene            complement(3021..>3355)
                     /locus_tag="WJL87_001858"
     CDS             complement(3021..>3355)
                     /locus_tag="WJL87_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ7800258.1"
                     /translation="EYDENSFIGKWYDDGVWDDEEYWKLENALIEVRRKYPYPMDIPR
                     DIVIGIGTIIEFLMVPNWKLFTIKSSPWLPKSVKIDERYERFRVMLRYIFTEIDIVNV
                     RFDYYNKK"
BASE COUNT          950 a          782 c          622 g         1001 t
ORIGIN      
        1 ctaccatgaa tatattcttt aactaatttc taagcttgaa attatgagac catatgctac
       61 taccatttat caacttttta ttttgtttat tgggagtgtt tttactatga cctcatgtga
      121 acctgttaat gaacaaacca gtttcaacaa tctcgagcca atgacaggat ttgaacatac
      181 ggttacattt gattttcagg gcatcaaaat ggttatccct tatggctatc ttgcacggta
      241 tacgcaagac aatgccacaa aatggctttc cgacacgccc gggcaggatg cttactccat
      301 taatttgata gagattagcg tctattacaa aaaaaccgac caaggctggg ttcttgagcc
      361 atacaaccag caaaacaaag cgcactttat ccaatttcta cgcgacggtt tggatagcgt
      421 ggacgatatt gttatccgaa aagatgcgtg tagtttaagc acgactatgg gagaaagatt
      481 gcttacttac ggggttaaaa aaatgccatc tgcctatcct gaatacgagg cttatgaaga
      541 taaaagacat attcctgaaa atccatattt tcatgaattt tactatatta aaaaaggaga
      601 aaatccggcg attattactc atcggaacta tcataggtat ggagagaacg attacagcac
      661 tagcgtaggt tcctgtatta acggtttcac ggtacggtat tacccgttta ttcgggaaaa
      721 gcagcagctc acacagcagg agttggtagg ttatcaccaa caagtagagc aattggtaca
      781 gagttttgta aacaattcaa gtaaaaaata atttaaagga tcttattatg aatgagggtg
      841 aagttgtttt aacaccagaa caaatccaaa ccttgcgtgg ttatgctttc cgtggcgata
      901 cctatggcgg ttggcgttat ttggctaatt tgggtgaccg ttatgcggat gatgctgctg
      961 caattgtcgg taaggatgca aacttaaatg gtttgaattt atggatgaaa aaaggtgtgg
     1021 aaaacctatg ggatgatacg gtcggtaaaa agacccgttt aatgtgtatt tccgtttttt
     1081 ggattgttgt tttcaatttg tagcgaatcg gattcggcat atacggcatt gcaaaaagcg
     1141 tttgactctc caatgccgtc tgaaaaccgg tttcagacgg catttgcgtt cagtgagaaa
     1201 ggtcgcgcct gccgcccgaa cgtctcgccg cagcctctgc ataacggcgc acctcttttt
     1261 ccaaattttc caagttcaaa ggaaaatcag gcagtctgtc tccctgtttc tcttcgcgga
     1321 taatccgccc gccatccaaa taccacgtct gttgcgcatg ataggtctgt atatccgccg
     1381 ttacgccatc cgctttcaat gctaccgtcg aagattgtgc aataaaaaga tttccgtttt
     1441 tcaaataata ttcgaaactc tggcgttttt ttccattgtc gaaactccaa tagactttct
     1501 gcggcagacc atccgcatca tagccgacca caagactgtt tgccttcatc ccccggggca
     1561 tcaattcccg catattctga taaaacacat aatcgcgcga gtccgatgca atccggttgc
     1621 tctcttcgcg gaagtcccaa accttctgct catcattcgc gacatcccgg tatttcgcca
     1681 aatatacctg ggccatctga taacacccga ggcaatgctc ataaacatct tccccgattt
     1741 tcccgcgccc tgccgcatca aataccgaac cgtccggttg ccaaacaacc cgatattctc
     1801 ctgtcgtttc ataattttcc ctgtgagccg ttccgccgta cacatttaca gaaaacggac
     1861 gatcgttccg atacagatat tcggcattaa caaacgcttc cggcgagcgt tgcaaaagcg
     1921 aaaccgcaac caaaccgccc tcgccgatat agtaatccag ccaaacctct tccccatgtt
     1981 cctgctccgt tacgtgaaac catttcgcct tttctttcaa acgactgagc cggatagcga
     2041 gcgcgagata atccttctcc gactgcaacg gaccgtcatc cacagtttcg gcaagacttt
     2101 cctccgtcct catcgattcc ttcacgatga caaccgccct gtcggcattt cggaacaggc
     2161 gggcaagttt cgccacaaaa gcattcggat tttcaggtac ttcagttgcc gtatcgctca
     2221 aaaaccaacg cggattaatc tcataggcaa tacccgttcc cagccaaaag gcaaatacaa
     2281 gtgcaaaaaa tgacaacagt accggtttga attttttaaa catatttatt tttcgtttaa
     2341 cagaatatat cgattatatc agacgagctt tgattgtttg atttttaatt taatctacgg
     2401 ttgctgccgt actttaaaaa cccttatgca ggctgcggct tgttactctt gtttgcttcg
     2461 gttcaatttt ttaactaatt caaatgcttc agatcgatat tgcctttgcc attttttcca
     2521 atctaacggt tttcccgagc gttcccaatc ttctaaaacc gacattttga acatttccat
     2581 ttcccaccaa tattcggcat ataaacgtga ttgattggga atgtcggctt ctgcccaatc
     2641 ggcaaaatgc aaaaaatgag aaatatcgca aactatatta ttttcaggaa cttcttcggc
     2701 aaatagatgc ttgactgact gtaacgcttc ggtctgatat ttttctgtcc atttctccca
     2761 tttttcaggc tttcccgata tttcccaatc ttccaatgcg gaagcattca agatttccaa
     2821 ttcccaccag caatcgtaat aacgagagga ttggttagga attttatttt ccgcttcgga
     2881 aacaaattgg gcaaaactaa gaatatcctt aacgattttt ttaaatctat tttccataaa
     2941 taattgccgc ccctccaatg aggaaaatgt cgtcaaaaat caaacacggt ttcagacggc
     3001 attttgattg ccgggttttg ctattttttg ttgtaataat caaatcgcac gttgactatg
     3061 tctatctcgg taaaaatata acggagcatt actctgaacc tttcataacg ctcatcaatt
     3121 ttgacacttt taggcaacca aggggaagat ttaattgtaa aaagtttcca atttggaacc
     3181 attaaaaact caataatggt accgattcca atcacgatat cccttggtat atccatcgga
     3241 taaggatatt ttctcctaac ctcgattaaa gcattctcca atttccaata ttcttcatca
     3301 tcccacaccc cgtcatcata ccatttgcca ataaatgaat tttcgtcata ctctt
//
LOCUS       DAWKST010000184         3343 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.184, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000184 DAWKST010000000
VERSION     DAWKST010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.184"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            60..689
                     /locus_tag="WJL87_001859"
     CDS             60..689
                     /locus_tag="WJL87_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ7800259.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLKDLGEEPYKFDKELPYE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALGLSPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            864..3242
                     /gene="hmbR"
                     /locus_tag="WJL87_001860"
     CDS             864..3242
                     /gene="hmbR"
                     /locus_tag="WJL87_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent hemoglobin receptor HmbR"
                     /protein_id="HEZ7800260.1"
                     /translation="MKPLQMLPIAALVGSIFGNPVFAADEAATETTPVKAEVKAVRVK
                     GQRNAPAAVERVNLNRIKQEMIRDNKDLVRYSTDVGLSDSGRHQKGFAVRGVEGNRVG
                     VSIDGVNLPDSEENSLYARYGNFNSSRLSIDPELVRNIDIVKGADSFNTGSGALGGGV
                     NYQTLQGRDLLLPERQFGVMMKNGYSTRNREWTNTLGFGVSNDRVDAALLYSQRRGHE
                     TESAGKRGYPVEGAGSGANIRGSARGIPDPSQHKYHSFLGKIAYQINDNHRIGASLNG
                     QQGHNYTVEESYNLLASYWREADDVNRRRNTNLFYEWTPESDRLSMVKADVDYQKTKV
                     SAVNYKGSFPIEDSSTLTRNYNQKDLDEIYNRSMDTRFKRITLRLDSHPLQLGGGRHR
                     LSFKTFASRRDFENLNRDDYYFSGRVVRTTSSIQHPVKTTNYGFSLSDQIQWNDVFSS
                     RAGIRYDHTKMTPQELNAECHACDKTPPAANTYKGWSGFVGLAAQLNQAWRVGYDITS
                     GYRVPNASEVYFTYNHGSGNWLPNPNLKAERSTTHTLSLQGRSEKGTLDANLYQSNYR
                     NFLSEEQKLTTSGDVSCTQMNYYYGMCSNPYSEKLEWQMQNIDKARIRGIELTGRLNV
                     DKVASFVPEGWKLFGSLGYAKSKLSGDNSLLSTQPLKVIAGIDYESPSEKWGVFSRLT
                     YLGAKKVKDAQYTVYENKGWGTPLQKKVKDYPWLNKSAYVFDMYGFYKPVKNLTLRAG
                     VYNVFNRKYTTWDSLRGLYSYSTTNSVDRDGKGLDRYRAPSRNYAVSLEWKF"
BASE COUNT          898 a          876 c          828 g          741 t
ORIGIN      
        1 gggcaagtgc ggattttgcc cgccccgcat ccttatccct taccgacaaa ggaaaactta
       61 tgagtgaaac cgaaaatcaa gccctgacgt ttgccaaacg tctgaaggcg gataccacgg
      121 cggttcacga cagcgtggat aacctcgtta tgtctgtcca accgtttgtc agcaaagaaa
      181 actacatcaa atttttgaaa ctccaatccg ttttccacaa ggccgtcgac cacatctata
      241 aagatgccga attaaacaaa gccattcccg agctggaata catggcgcga tacgatgccg
      301 taacgcaaga cttgaaagat ttgggcgaag agccttacaa attcgacaaa gaattgccat
      361 acgaaactgg caacaaagca atcggctggc tttattgcgc cgaaggatcc aatttgggcg
      421 cggcgttttt gttcaaacac gcccaaaaac tcgattacaa cggcgaacac ggcgcgcgcc
      481 acctcgctcc gcatcccgac gggcgcggca aacactggcg cgccttcgtc gagcatctga
      541 acgcattggg cttaagcccc gaagccgaag ccgaagccat ccaaggcgcg cgcgaagcct
      601 ttgcattcta caaagtcgtg ttgcgcgaaa ccttcggctt ggcagccgat gccgaagccc
      661 ccgaaggtat gatgccgcac aggcactaaa aaataatcga accaaataaa caaggtctcg
      721 gcatagctgt ttgcagggac ctttaattac acggcgcggc tttgtttaca tggattactg
      781 tcttattaaa tattaatgat tatcataaaa tctattattc gctaaccgat ggatgaacaa
      841 tccatacatc ttgagttgat aatatgaaac cattacaaat gctccctatc gccgcgctgg
      901 tcggcagtat tttcggcaat ccggtctttg cggcagatga agctgcaact gaaaccacac
      961 ccgttaaggc agaggtaaaa gcagtgcgcg ttaaaggcca gcgcaatgcg cctgcggctg
     1021 tggaacgcgt caaccttaac cgtatcaaac aagaaatgat acgcgacaac aaagacttgg
     1081 tgcgctattc caccgatgtc ggcttgagcg acagcggccg ccatcaaaaa ggctttgctg
     1141 ttcgcggcgt ggaaggcaac cgtgtcggcg tgagcataga cggcgtaaac ctgcctgatt
     1201 ccgaagaaaa ctcgctgtac gcccgttatg gcaacttcaa cagctcgcgt ctgtctatcg
     1261 accccgaact cgtgcgcaac atcgacatcg taaaaggggc ggactctttc aataccggca
     1321 gcggcgcctt gggcggcggt gtgaattacc aaaccctgca aggacgtgac ttactgttgc
     1381 ctgaacggca gttcggcgtg atgatgaaaa acggttacag cacgcgtaac cgtgaatgga
     1441 caaataccct cggtttcggc gtgagcaacg accgcgtgga tgccgctttg ctgtattcgc
     1501 aacggcgcgg ccatgaaact gaaagcgcag gcaagcgtgg ttatccggta gagggtgctg
     1561 gtagcggagc gaatatccgt ggttctgcgc gcggtattcc tgatccgtcc caacacaaat
     1621 accacagctt cttgggtaag attgcttatc aaatcaacga caaccaccgc atcggcgcat
     1681 cgctcaacgg tcagcagggg cataattaca cggttgaaga gtcttacaac ctgcttgctt
     1741 cttattggcg tgaagctgac gatgtcaaca gacggcgtaa caccaacctc ttttacgaat
     1801 ggacgccgga atccgaccgg ttgtctatgg taaaagcgga tgtcgattat caaaaaacca
     1861 aagtatctgc ggtcaactac aaaggttcgt tcccgataga ggattcttcc accttgacac
     1921 gtaactacaa tcaaaaggac ttggatgaaa tctacaaccg cagtatggat acccgcttca
     1981 aacgcattac cctgcgtttg gacagccatc cgttgcaact cggggggggg cgacaccgcc
     2041 tgtcgtttaa aactttcgcc agccgccgtg attttgaaaa cctaaaccgc gacgattatt
     2101 acttcagcgg ccgtgttgtt cgaaccacca gcagtatcca gcatccggtg aaaaccacca
     2161 actacggttt ctcactgtct gaccaaattc aatggaacga cgtgttcagt agccgcgcag
     2221 gtatccgtta cgatcatacc aaaatgacgc ctcaggaatt gaatgccgag tgtcatgctt
     2281 gtgacaaaac accgcctgca gccaacactt ataaaggctg gagcggtttt gtcggcttgg
     2341 cggcgcaact gaatcaggct tggcgtgtcg gttacgacat tacttccggc taccgtgtcc
     2401 ccaatgcgtc cgaagtgtat ttcacttaca accacggttc gggtaattgg ctgcccaatc
     2461 ccaacctgaa agccgagcgc agcaccaccc acaccctctc tctgcaaggc cgcagcgaaa
     2521 aaggtacttt ggatgccaac ctgtatcaaa gcaattaccg caatttcctg tctgaagagc
     2581 agaagctgac caccagcggc gatgtcagct gtactcagat gaattactac tacggtatgt
     2641 gtagcaatcc ttattccgaa aaactggaat ggcagatgca aaatatcgac aaggccagaa
     2701 tccgcggtat cgagctgacg ggccgtctga atgtggacaa agtagcgtct tttgttcctg
     2761 agggctggaa actgttcggc tcgctgggtt atgcgaaaag caaactgtcg ggcgacaaca
     2821 gcctgctgtc cacccagccg ttgaaagtga ttgccggtat cgactatgaa agtccgagcg
     2881 aaaaatgggg cgtgttctcc cgcctgacct atctgggcgc gaaaaaggtc aaagacgcgc
     2941 aatacaccgt ttatgaaaac aagggctggg gtacgccttt gcagaaaaag gtaaaagatt
     3001 acccgtggct gaacaagtcg gcttatgtgt tcgatatgta cggcttctac aaaccggtga
     3061 aaaacctgac tttgcgtgca ggcgtatata atgtgttcaa ccgcaaatac accacttggg
     3121 attccctgcg cggcctgtat agctacagca ccaccaactc ggtcgaccgc gatggcaaag
     3181 gcttagaccg ctaccgcgcc ccaagccgta attacgccgt atcgctggaa tggaagtttt
     3241 aatctggtat tattgaatta atcgccttgt tgaaaattaa agccgtccga attgtgttca
     3301 agaactcatt cggacggttt tttaccgtaa atctgtgtga tgg
//
LOCUS       DAWKST010000185         3332 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.185, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000185 DAWKST010000000
VERSION     DAWKST010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.185"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..408
                     /gene="mafB"
                     /locus_tag="WJL87_001861"
     CDS             <1..408
                     /gene="mafB"
                     /locus_tag="WJL87_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ7800261.1"
                     /translation="GDFSDSYKHNTASRLSQSVDGEMFQTRNVDFKAKSIGTKIHDGA
                     QGKHISGHRNYIEGKSTLNQNINPQELLNGIHSGAYPVISKGARGNPVVDFGYPIGSD
                     GKSGLSTNFGTIHSGKNGVHIVPANPKTIKKVQ"
     gene            411..728
                     /locus_tag="WJL87_001862"
     CDS             411..728
                     /locus_tag="WJL87_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800262.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEERRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            862..1569
                     /locus_tag="WJL87_001863"
     CDS             862..1569
                     /locus_tag="WJL87_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ7800263.1"
                     /translation="MVKTADGYKAIAHIQAGDRVFAKDEASGKTGYKPVTARYGNPYR
                     ETVYIEISDGIGNNQTLISNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGDKAETEGVWVHNECPYGEKYRTEVGSYTNTHESGRT
                     YSGKGTRQRSQISGAREARVNNDPHIATDFTPAKNNREAFKDESRRIDAHGGSKSTNN
                     YNRIESPGKKYRKQDGD"
     gene            1572..1895
                     /locus_tag="WJL87_001864"
     CDS             1572..1895
                     /locus_tag="WJL87_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEZ7800264.1"
                     /translation="MIVDEKSTSDVLKQYQELGFDLTKSMIIEFFIEGSQKNLQSIES
                     QIISYRQDFQISIEQNEFGEMWTCYCSVNIIPSLENILAIETTLFDIAQKNDCNYEGF
                     GSYGN"
     gene            2128..2400
                     /locus_tag="WJL87_001865"
     CDS             2128..2400
                     /locus_tag="WJL87_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800265.1"
                     /translation="MMSVKELFKVILDENKDFPIRTIHRTPMGILPKPVALSIVQYEN
                     DPGFYLFYLDETGQEQTDTYHDTLDSAFEQAEFEFGISKEEWMQSP"
     gene            2521..3066
                     /locus_tag="WJL87_001866"
     CDS             2521..3066
                     /locus_tag="WJL87_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ7800266.1"
                     /translation="MIIVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFE
                     QFLLDNEVGNLSDMNRKPFTW"
     gene            3169..3332
                     /locus_tag="WJL87_001867"
                     /pseudo
     CDS             3169..3332
                     /locus_tag="WJL87_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1110 a          610 c          691 g          921 t
ORIGIN      
        1 ggggatttct cagactccta caagcataac actgcttcaa gattatctca gtctgtagat
       61 ggagaaatgt ttcaaacccg caatgttgat tttaaagcaa aatctattgg gactaaaatt
      121 catgatggag ctcaagggaa acatatttca ggacatagaa actacattga aggtaagagt
      181 actttaaatc aaaacattaa tcctcaagaa ttgttgaacg gaatacattc aggtgcttat
      241 ccagttattt ctaaaggagc aagaggaaat cctgttgttg attttgggta tcctataggc
      301 agcgatggga aatcaggatt aagtaccaat tttggtacga ttcattcagg taaaaatgga
      361 gttcacattg ttccggctaa ccctaaaacc attaaaaagg tgcaatagtt atgaatatat
      421 taccaagctg gctgcgagtc ggtatgaata tagcaatgct gggcatgata cactcagata
      481 tcaggttaat taccgtagat tacgaggaaa gaagaaggtt tttaaaaatc aaaaattatt
      541 tatcaagaga agccatcaca gaagaccatg aagatatgga atatttgatt acagagttat
      601 ggtctatgtg tggagaatat tttgatgaag ctgactttga atgtatttat tctaatcatt
      661 cttctatgga gttaaaccaa ataaatggtg cagtattcag gagaaaggaa ttaatttcgc
      721 aagcgtaggt taaaaaacca acaatcacaa tgtcttctga aaccgtgttt aattttcaga
      781 cggcatttcc ttcatttgaa ataggatatt gagaactgag ttcttcaaaa atcctacacc
      841 tgctccttcc acggcagcac cttggtcaaa acggcagacg gctacaaagc cattgcccat
      901 attcaagccg gcgaccgcgt cttcgccaag gacgaggcaa gcggaaaaac gggatacaaa
      961 cccgttaccg cccgatacgg caatccgtat cgggaaaccg tttacattga aatttcagac
     1021 ggcatcggca acaaccaaac cctgatttcc aataaaatcc acccgtttta cagtcaagga
     1081 aaatggatac aggcaggtcg tctgaaaaaa ggcgacaccc tgctttccga aagcggtgca
     1141 aaacagacgg ttcaaaacat taccctcaaa cagcagccgc tcaaagccta caacctgacc
     1201 gttgccgact ggcatactta cttcgtcaag ggtgataagg cggaaacgga aggggtttgg
     1261 gttcataatg agtgtccgta tggagaaaaa tataggacag aagttggttc ttatactaat
     1321 actcatgaaa gtggtcgaac ttattctgga aagggcacaa gacaacgttc ccaaatctct
     1381 ggagcaagag aagctagggt taataatgac cctcatatag ctacagattt tactcctgcc
     1441 aaaaataatc gagaggcatt caaagatgaa tctagaagaa ttgacgctca cggtggttcc
     1501 aaaagtacca ataattacaa tcgaattgaa agcccgggga aaaaatatcg aaaacaagat
     1561 ggagattaaa aatgattgtt gacgaaaaat ctacttcaga tgtactgaaa caatatcaag
     1621 aactagggtt tgatttgaca aaatcgatga taatagagtt ttttatagag ggttctcaaa
     1681 agaatctgca atcaatagaa agtcaaatca tttcttatcg gcaagatttt caaatcagta
     1741 ttgagcaaaa tgaattcgga gaaatgtgga cttgctattg ttccgtcaat attatccctt
     1801 ctcttgaaaa cattttagct attgaaacta cgttatttga tattgcacaa aaaaatgatt
     1861 gtaattatga aggttttggt tcgtatggga actaacaaga agtgtaggtt gggttgaaaa
     1921 acacaacaat caaaaggtcg tctgaaacca tgtttaattt tcagatggct tttccttcat
     1981 ttaaaacagg atattgagat ctaagttctt taaaaatcct acacctgctc cttccacggc
     2041 agcaccttgg tcaaaacggc agacggctac aaagccaatg gacgtgtaat agaaaatagt
     2101 gcggtaacaa ttattcggga ttgaagaatg atgagtgtaa aagaattatt caaagtcatc
     2161 ttagatgaaa ataaagattt tcctattaga acgattcatc gtaccccgat ggggatactt
     2221 cccaaacctg ttgctttaag tattgttcaa tatgaaaatg acccgggatt ttatttgttt
     2281 tatttggatg agaccggtca ggaacaaacg gatacttatc atgacacatt agattcggca
     2341 tttgaacagg ctgaatttga atttggaatc agcaaagagg aatggatgca aagtccttag
     2401 acggcaaaat caagatgccg ctttattttt cagacggcat ttccgtcaat aaaaaagtcg
     2461 gtattggagc ccaaaacagc ctacgtcttg ccgggatagg gatgaaatct ttaagaaaaa
     2521 atgattatag ttgaacattt agaacattat ttgggtgaaa tcgaaagcgg tataaaatgc
     2581 ttggatcgac gttatcatct cagcgtttcc gtcttcccat cacaaccata taaaggggta
     2641 actacttttt ctactttagg cttgaatcga tatgacttga actataagag taggtttgaa
     2701 ttaattttta cctgttctga agaatggaat aaagaaaata ttgcagcttt tctatcagga
     2761 gtggctgaat atttgattga caatagacaa cctattttaa gaggtgaaat tattcaatta
     2821 cccagggtaa ttatcgaagg tagcaaaatg gatgcactgt atgtttccgc cccattttat
     2881 ttcgacgatg attttcaggt ctgttatggc gaacactaca atatcgtttt ccctttgctt
     2941 gtcccgttgt ataaacagga agcagaattg gtggaaaaaa agggttggaa tgcttttgag
     3001 cagttcttgc tggataatga agttggcaac ctttcggata tgaatcggaa accgtttact
     3061 tggtaaggat tttcttaaat ttaatcaaag tgccgtctga aaccgtgttt aattttcagg
     3121 cgaccttccc cttattttaa acaggatatt gggaactaag ttcttcaaaa atcctacacc
     3181 tgctccttcc acggcagcac cttggtcaaa acggcagacg gctacaaagc cattgcccgt
     3241 atccgaaccg gcgaccgcgt cttcgccaag gacgaggcaa gcggaaaaac gggatacaaa
     3301 cccgttaccg cccgatacgg caatccgtat ca
//
LOCUS       DAWKST010000186         3311 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.186, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000186 DAWKST010000000
VERSION     DAWKST010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.186"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(316..618)
                     /locus_tag="WJL87_001868"
     CDS             complement(316..618)
                     /locus_tag="WJL87_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800267.1"
                     /translation="MGYRVGMNCFDTRLQADDYLLSSLPPTVTEEGKIIRPERVGDKW
                     ILNGKPVTLSYPECSNFEQIKQGSYVGSTVLILFVVIFGFRLLINFLKDMGKVGTD"
     gene            complement(646..876)
                     /locus_tag="WJL87_001869"
     CDS             complement(646..876)
                     /locus_tag="WJL87_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800268.1"
                     /translation="MNIVKKYAVKALLAAGIFTPAIVMADGFDAAAIGTQVANVIMGF
                     VAMVSAVGMAAITVILAIQGFKMAWSMIKSVK"
     gene            complement(1114..1314)
                     /locus_tag="WJL87_001870"
     CDS             complement(1114..1314)
                     /locus_tag="WJL87_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800269.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEMGGGFQIFQFFVKSE"
     gene            complement(1318..1629)
                     /locus_tag="WJL87_001871"
     CDS             complement(1318..1629)
                     /locus_tag="WJL87_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800270.1"
                     /translation="MFNQNQTVTYPATFLGAKKFKGEIDGSNIDTCSVLVATPLPAQS
                     GNAVGFTAAQMKFGDSKNFSRLENLKYPCEVMVTVEMTSTGKGMVPSLIDFQVAEKPK
                     G"
     gene            complement(1641..2855)
                     /locus_tag="WJL87_001872"
     CDS             complement(1641..2855)
                     /locus_tag="WJL87_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ7800271.1"
                     /translation="MSEVEYFSHFISDGKGKLLEIPQRRGKQDGVFVDWISFTFHEDT
                     LLKVSGCPLFSDAEYMYVLSRKLEEILGFGITRKCKSRGNKFYESMYRLGSDDVDYGE
                     VHFGGQRNTVLVELKGTGCSVASPGWELRLKQFLDDSIRTRITRIDLALDFFDGEYTP
                     DQALLDHDNGFFDNSNQRPKSETIGTAWRNEDGSGKTFYVGRKKNSRFVRVYEKGRQL
                     GDKESKWVRFEIQFNYGDIEIPLDILINQGSYFCGAFPICRKFKNMPVPERFDQRKKT
                     LNLTFEHKLHYAKNAVGKLVNFMIEMGFDNSEIVESLKADSGFPKGLEPEKYALEMLR
                     DGLKHGFIHEQPDIDLEIELDELGVIAFKNSDKFDREKRLFSPDYDAEKERKYQDYLD
                     EVYHQNVNYDYF"
BASE COUNT         1046 a          775 c          505 g          985 t
ORIGIN      
        1 tgtagaagaa tcagggaatt gaatttctat aaaatctcta tcataacgat gaaagaaatt
       61 cctaaaaata tcaaatgaaa acgaagtttt aaaaacccct tttctaatca attccctata
      121 ataaattaac gcttgtggta aatctctata aatatgtgaa tcagtttgaa atttaactaa
      181 atgatattca gaaataaaca taattcggac cttttaaaac actctaaaaa gccgattcta
      241 ccaaccgtta ccaaaacagc caagcaacag acaaagccaa gaagaaaccg agaagaaacc
      301 aaaaatcaat aatcatcaat cagtaccaac cttgcccatg tcttttaaga aattaatcag
      361 aagcctgaaa ccgaaaatga ctacgaacag aattagaacc gtcgaaccga cataagaacc
      421 ttgctttatc tgctcaaaat tggaacattc cggataagac aacgtaaccg gctttccgtt
      481 caaaatccat ttatcgccca ccctttccgg cctgatgatt tttccttctt ctgtaacagt
      541 aggaggaaga gacgacaata aatagtcgtc tgcctgcaat cttgtatcaa aacagttcat
      601 gccgacacga taacccattc acgcgcccct ctttcttcac tgccgttatt tgacagattt
      661 aatcatgctc caagccattt tgaagccttg gattgccaaa atgacggtaa tggccgccat
      721 acccacggcg gaaaccatcg caacgaaacc catgattaca ttcgccactt gcgtaccaat
      781 cgcggctgca tcaaagccat ctgccataac aatagccggt gtgaagatac cagctgccaa
      841 caatgctttt acagcatatt ttttaacgat gttcatcgtt tttttccttt ttttgatatt
      901 taaaataaga cgacttcttg acttgcttca tccggacgaa gtcttttccg aatctcgttt
      961 ttagccgata aaatagagga ttgcgaaaag aaaaagaaac atacagacca cccagccgat
     1021 aattagtgtt gcaaggttca ttttcatgat atttttcctt tgttgcgggc tttgtgaaag
     1081 gttaacagac cgcccgccga gcctgttttt cttttattcc gattttacga agaactgaaa
     1141 tatctggaat cctccgccca tttcatttat gcctgaattc aatgcatctt cgtaactttc
     1201 aaattgacct gctgatttaa tattttgagt aaaccccaca tcaccgaacg gatcgggata
     1261 aataaagtca tgcgtttcta aatcttgaac tatgaaacgt tcttcaaatt tcataaatca
     1321 acctttcggc ttttccgcca cctgaaaatc aattaatgaa ggaaccatgc ctttaccagt
     1381 cgaagtcatt tcaaccgtta ccataacttc gcacggatat ttgagattct ctaaccttga
     1441 gaaattctta ctatccccga acttcatttg tgctgccgta aatccaacag cattacccga
     1501 ctgtgccggt aaaggtgttg caaccaatac ggaacaagtg tcaatgttag agccatcaat
     1561 ttcacctttg aattttttgg ctcccaaaaa agtcgcagga taagttacag tttggttttg
     1621 attaaacatt ttgatttcct ttaaaaataa tcataattta cattttgatg ataaacttca
     1681 tctagataat cctgatattt cctttctttc tcggcatcat aatcaggact aaaaagcctt
     1741 ttttccctat cgaatttgtc agaattttta aaagcaataa cccccaattc atcaagttca
     1801 atttccaaat caatatccgg ctgttcatga ataaaaccgt gtttcaaacc gtcccttaac
     1861 atttccagag catatttttc aggttctaat cctttgggaa atcccgaatc tgcctttaaa
     1921 gattccacaa tttcgctatt atcaaaaccc atttcaatca tgaaattgac cagttttcca
     1981 accgcgtttt tcgcgtaatg caatttatgc tcgaaagtta aattaagcgt tttctttctc
     2041 tgatcaaacc tttcgggaac cggcatattt ttaaattttc tacaaattgg aaaagctcca
     2101 cagaaatacg aaccctgatt tattaaaata tccaagggta tttctatatc tccataatta
     2161 aactggatct cgaaccttac ccatttgctt tctttatctc caagctgcct gcctttctca
     2221 taaacacgaa caaaacgaga atttttcttg cgacctacat aaaatgtctt gccgctcccg
     2281 tcctcattcc gccaagccgt accgatcgtt tcagatttcg gcctttgatt gctgttatca
     2341 aaaaaaccat tatcgtgatc taacaacgcc tgatccggcg tgtactctcc atcaaaaaaa
     2401 tcaagtgcta ggtcaattcg cgttattctt gtccttatcg aatcatcgag aaactgcttt
     2461 agcctcaact cccaacccgg acttgcaacg ctgcaaccag tacctttcaa ctcaactaaa
     2521 acagtattgc gctgacctcc gaaatgcacc tctccataat caacatcatc cgaacctaac
     2581 ctatacatgg attcatagaa tttgttgccc cttgatttgc atttgcgcgt tatgccaaaa
     2641 cctagaattt cttccagctt tctgcttaat acatacatgt attcagcatc agaaaataaa
     2701 gggcaaccgg aaactttcag taaagtatct tcatggaatg tgaatgaaat ccaatcaaca
     2761 aaaaccccgt cttgcttacc tcttcgctgc ggaatttcta aaagcttccc ttttccgtcc
     2821 gatataaagt gtgagaaata ttcaacttca ctcattcgtt cactactctt ggaattggtt
     2881 tttaatttcg ctccccccct gttagtcaag ggggggcttt cagccgtttc ccgtcttccg
     2941 cgctaaagcg cgtacgacgg tcaacgaccg aaagccaaat cctgacaaac ttttaaagat
     3001 caacaaaaaa gaccacaacc atccgttgtg ataatcactg gaaaattcga accaaccgaa
     3061 tctatataat cgaacgcctg ataaagcttt gaaaaattct cttgattaat gagtttatgc
     3121 ggttcgccat gtctgaactg ataaaaacac aaaacacaag aatctgattt tttaaatatt
     3181 ctccaataag aacaggaaaa tattacattt actgctgaca taaaaaagcc cctttcactt
     3241 ggctgtcaaa ggggaatgtt aagaaaatta atgcgcccct ttgatagagc gcatcatata
     3301 aggcgggaat c
//
LOCUS       DAWKST010000187         3284 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.187, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000187 DAWKST010000000
VERSION     DAWKST010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.187"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(96..320)
                     /locus_tag="WJL87_001873"
     CDS             complement(96..320)
                     /locus_tag="WJL87_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800272.1"
                     /translation="MVFWLQRLKYPLLLWIADMLLYRLLGGAEIECGRCPVPPMTDWQ
                     HFLPAMGTVSAWVAVIWAYLIIESEKNGRY"
     gene            423..2573
                     /gene="dinG"
                     /locus_tag="WJL87_001874"
     CDS             423..2573
                     /gene="dinG"
                     /locus_tag="WJL87_001874"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ7800273.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQNNAQQNLLGFEAPEVLWD
                     SKPKPEELKLLHDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSLGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAVAVRSLLKHVIGLNDALS
                     AARREKEQDGALLDRLTGDFGIFIARIEQISAVWDLLSTVPLEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAIIEWLPKLISPGEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRVKTQRYGQQLLAGLPPFKRIG"
     gene            2609..3223
                     /locus_tag="WJL87_001875"
     CDS             2609..3223
                     /locus_tag="WJL87_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEZ7800274.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGAPVAGAASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIGDIATAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGF"
BASE COUNT          822 a         1078 c          806 g          578 t
ORIGIN      
        1 gcacatcctg tcgccggtat tccctaagcg gtacggcaac ctaaaacaaa tgccgtctga
       61 acttccgttt cagacggcat ttttatacgg actactcaat atcttccgtt tttttcactt
      121 tcaataatca gatatgccca aatcaccgcc acccaagccg acaccgttcc catcgccggc
      181 aaaaaatgct gccaatccgt catcggcggc acagggcaac ggccgcattc gatttccgcg
      241 ccgcccaaca accggtacag caacatatcc gcaatccaaa gcaacaacgg gtatttcaaa
      301 cgctgcaacc aaaaaaccaa ccacgtccgc acgattcacc tcacccccca ttccgacaac
      361 gatattgtac cgcttccgtg caatgttaaa ataagcccac ttctaccgcc gtacaaaacg
      421 ccatgctcac cgatttagaa aaaaacgcca tccgcgacca ttaccaaaac atcggcaaaa
      481 acctgcccgg tttccgtccg cgcgcttcgc agcgggaaat gattgcggcg gttgccaacg
      541 ctttttcgcg gacgttggcg cgcgaagaag gcggcgagcc gcccaagcgc gagggcgaga
      601 gcatcgccgt aatcgaaggg ccgaccggcg tgggcaaatc gcttgcctac ctgctggcgg
      661 gcggcatcat ggcgcagacg cgcggcaagc ggctgattgt gagcagcgca acggttgcct
      721 tacaggagca gttggttgac cgcgacctgc cgtttctggt cgaaaaaagc ggtttggaac
      781 tgaccttcgc gcttgccaaa gggcgcggcc gctatctctg cccttacaaa ctctaccaac
      841 tgacgcaaaa caacgcccag caaaacctgc ttggttttga agcaccggaa gtattgtggg
      901 acagcaaacc caagcccgaa gaattgaagc tgctgcacga cattgccgac gaattttccg
      961 cccgacggtt caacggcgac cgcgacactt ggccggaaaa aatcgatgac gcgatttggc
     1021 tcaaagttac caacgaccgc cacggctgcc tgaaaaccgc ctgtcccaac cgtccggaat
     1081 gtccgtttta cctggcacgc gatgtcttgg aaaccgtcga tgtcgtcgtt gccaaccacg
     1141 accttctgct tgccgacatc agtatgggcg gcggcgtgat tctgcccgcg cccgaaaaca
     1201 gtttctattg catagacgaa gcgcaccacc tgcccaaaaa agccctcagc cgttttgccg
     1261 ccgaacattc gtggaatatt gccgtttgga cgctggaaaa actgccgcag ctgaccggca
     1321 agattgccgc gcttaccgat aaagccgaac ttgccaacct tgccgatgaa gccgccgcat
     1381 ccttgctcga cagcctgcac gaatggcagt tccatttggc ggaagagccg tctttgagct
     1441 tggggccgtc tgaaaacgac aggcgaacca acagcgaacc gacttggctg tgggaagacg
     1501 gcaaaatccc cgaaggcctc gaaaccaccg tttccaacac cgccgttgct gtgcgcagcc
     1561 tgctcaaaca cgttatcggg ctgaacgatg cgctttctgc cgcacgccgc gaaaaagaac
     1621 aggacggcgc gctcctcgac cgcctgaccg gcgatttcgg catcttcatc gcccgtatcg
     1681 aacaaatcag cgcggtttgg gatttgctct ccaccgtccc cctcgaaggc gaagagccgc
     1741 tggcaaaatg gataacccgc cgcgccgacg acaaaaacga ctacattttc aacgccagcc
     1801 ccatcagcag cgcgtcccac ctcgccaaca gcctgtggcg gcgcgcggca ggcgcggtat
     1861 tgacctccgc caccctgcaa tccttgggca actttaacct gatgctgcgc caaaccgggc
     1921 tgcaatggct gcccgagacc accaccctcg ccctcaaaag cccctttgac tttgaaaaac
     1981 agggcgaact ctacatcccc cccatatacg ccagccccaa agaccccgaa gcccacaccg
     2041 ccgccatcat cgagtggctg cccaagctca tttcgcccgg cgaagccatc ggcacgctcg
     2101 tcttgttttc ctcgcgcaaa caaatgcagg atgtcgccct gcgcctgccc ggagactacc
     2161 tgccgctctt gctcgtacaa ggcgaattac ccaaagccgt tctcctgcaa aaacaccacc
     2221 gggccataga agaaggcaaa gccagcatca tcttcggact cgacagcttt gccgaaggac
     2281 tcgacctgcc cggcaccgcc tgcgtgcaag tcatcatcgc caaacttccc ttcgccatgc
     2341 ccgacaaccc catcgaaaaa acccaaaacc gctggataga acagcgcggt ggcaaccctt
     2401 tcatcgaaat caccgtcccc gaagccggca tcaaactcat ccaggccgtc ggccgcctca
     2461 tccgcaccga acaagactac ggccgcgtaa ccatcctcga caaccgcgtc aaaacacagc
     2521 ggtacggcca acaattatta gccggcctgc caccgtttaa aaggataggg taaacctacc
     2581 gccctgcccg caacagaaag gaaaaatcat gaacttcacc cgactgctca accaagtctt
     2641 aagcacggtt caaaaaaaag gcaacacctt ctccgacagc ccgctcaatt cattcggcgg
     2701 aggcgcgccg gttgccggtg ccgcgtccat gctgctgaac ggtaaaaacc gcaaaaccat
     2761 taccaaaatc ggttcgaccg ccgctttggg ctacctcgcc taccggggct atcagatgtg
     2821 gcagcaaaac aaagggcggg caaccgtaac acaaagcgat ttccaacctg ccggagaaac
     2881 tgaagaaaca tacagccgca ccgtattgcg caccatgata gccgccgccg cttcagacgg
     2941 catgatagac gaagccgaac gccggactat cgaacaggaa agcggcacag accccgaaac
     3001 tgccgcatgg ctcgccgccg aataccgcct gcccgcaagc atcggggaca tcgccaccgc
     3061 cgtcggcaac gatgaggcgt tggcggcgga ggcctatctg gcggcaaggt tggtctgtgc
     3121 cgatttgtcg cggaaagaaa ccgtcttcct cgcccgcctg tcgcaggctt tgaaactgga
     3181 tgacaatctg gtggagagtt tggaaaggca attggggttt tgatgacaca gccagggagg
     3241 gaaaatcatc aaaaaacggt tattccgacc ccggcctcaa aacg
//
LOCUS       DAWKST010000188         3253 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.188, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000188 DAWKST010000000
VERSION     DAWKST010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.188"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(12..1307)
                     /gene="tyrS"
                     /locus_tag="WJL87_001876"
     CDS             complement(12..1307)
                     /gene="tyrS"
                     /locus_tag="WJL87_001876"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ7800275.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKISLYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     SQLTPFLSFEGGNAAIMANNADWFGKMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLYQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAIEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLNGEHKRFGKYTILRRGKRNHALLVWK"
     gene            complement(1370..3247)
                     /locus_tag="WJL87_001877"
     CDS             complement(1370..3247)
                     /locus_tag="WJL87_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ7800276.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKNWLPGGFLGVDIFFV
                     ISGFLMTMILHREMSGGGRFSLKAFYIRRIKRILPAFFAVLAATLIGGFFLFTKDDFF
                     LLWKSALTALGFASNLYFARGKDYFDPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVA
                     RKSPRVQFGFLAALCALSLAASFMPSAFDKYYLPHLRACEMLVGSLTAVWMRYRQQRN
                     LAVGRCCAAAGALFSACILSACLFAYSKQTAYFPGPAALIPCLAVAALIYFNHYEHPL
                     KKFFQWKITVAAGLISYSLYLWHWPILAFMRYIGPDNLPPYSTAAAIVLILLLSLISY
                     HCIEKPFKKWKGSFAQSVLWIYALPMLILGAGSFFAMRLPFMAQYDRLGLTRSNTSCH
                     NNTGKQCLWGDTEKQPELLVLGDSHADHYKTFFDAVGKKEKWSATMVSADACAYVEGY
                     ASRVFQNWAACRAVYRYAEEHLPLYSKVVLAMRWGSQMPENSRSLAYDAGFFQKFDRM
                     LHKLSSEKQAVYLMADNLASSYNVQRAYILSSRIPGYRQTLRPDDESTLKANARIREL
                     AAKYPNVYIIDAAAYIPANFQIGGLPVYSDKDHINPYGGTELAKRFSEKQRFLDTRHN
                     H"
BASE COUNT          764 a          783 c          963 g          743 t
ORIGIN      
        1 cggcaatcgg attatttcca aaccaaaagc gcgtggttgc gtttgccgcg ccgaaggatg
       61 gtgtatttgc cgaaacgttt gtgttcgccg ttcagcaggc aggcatcgtc ggggcgttcg
      121 gcggcgtggt tggggttgtt ggcttcggca ggtttgccgt tgagcaaaac cgctttgctg
      181 ttcacaaagc cgcgcgcttc tttattggag gatgccaaac cggttttgac caaggcttcg
      241 acgacattga tgccgtctga aacttcaaat gcaggcaggc cgtcgagggc gagctgctcg
      301 aagtcgcttt cggtcaggct gctttggtct tcggcaaaca ggctttcgga aatgcgctgc
      361 gcggcggcaa gggcttcttc gccgtgaatc aggcgggtca tttcttcggc gaggatgcgt
      421 tgcgcttcgg gcttgctgcc gctggcttta tctttcgctt cgatagcgtc gatttcttcg
      481 atggacagga aggtaaagta tttcaggaat ttatacacat cggcatcggc gactttcagc
      541 cagaattggt agaactgata gggcgaggtt tttttcgcgt tcagccacac cgcgccgcct
      601 tcggttttgc cgaatttggt gccgtctgat ttggtaacca aaggcagagt cagtccgaac
      661 acttgttttt ggtacaggcg gcgggtcagg tcgataccgg cagtgatgtt accccattgg
      721 tcggagccgc cgatttccaa aaccgcgccg tggcgtttgt tcaactcggc gaagtcgtaa
      781 ccttgcagca gggaataggc gaactcggtg aaggaaatgc ctgcgccgtc gcggtcgatg
      841 cgctgtttga cggattcttt gttcagcatg gcgttgacgg agaaatgctt gccgatgtcg
      901 cgcaggaagt cgaggcagtt cattttgccg aaccagtcag cgttgttcgc cataatggcg
      961 gcgtttccgc cttcaaagct caagaaaggg gttaattggc tgcggatgct ttccacccag
     1021 ccggcaacgg tttcggcgga attcaagctg cgttcggcgg ctttgaagct ggggtcgccg
     1081 atcataccgg tcgcaccgcc caccagtgca atcggcgtat gccccgcctg ttggaagcgg
     1141 cgcaatgcca atacgggcag caggtgtccg atgtgcaggc tgtcggcggt cgggtcgaag
     1201 ccgcaataaa gggaaatttt ttgttcgttc aacaaagcgt ctaaggcttc gatgtcggtg
     1261 gtttgcgcga ttaggccgcg cgattgcagg tcttggatga cgctcatcgg tctctttcaa
     1321 aaaaaattag cggttttgca aaccgccgat tgtaacaaat ttaagcgaat caatggttat
     1381 ggcgcgtatc gagaaaccgt tgtttttcgg aaaaacgctt cgccaattcc gtgccgccgt
     1441 aagggttgat gtggtctttg tccgagtaaa ccggcaatcc gccgatttgg aaatttgcgg
     1501 ggatataggc ggcggcatca ataatataga cgttggggta tttggctgcc aattccctga
     1561 tgcgtgcatt ggctttcaga gtgctttcgt cgtccgggcg cagggtttgg cggtaacccg
     1621 gtatgcgtga agacaagata taggcgcgtt gtacgttgta agacgaggca aggttgtccg
     1681 ccatcaggta aacggcttgt ttttcggatg agagtttgtg cagcatacgg tcgaattttt
     1741 ggaaaaaacc ggcatcgtag gcaagggagc ggctgttttc gggcatttgg ctgccccagc
     1801 gcatcgccaa aaccactttt gaatacaggg gcaggtgttc ttcggcatag cggtaaacgg
     1861 cgcggcaggc ggcccagttt tggaacacac gggacgcgta gccttccaca taggcgcaag
     1921 cgtcggcgga aaccatagtg gcggaccatt tttctttttt gcccacggca tcgaagaatg
     1981 ttttgtaatg gtcggcgtgg gagtcgccca aaaccagcag ctccggctgt ttttccgtat
     2041 ccccccacag gcattgtttg ccggtattgt tgtggcagga ggtgttggaa cgcgtcagcc
     2101 ccaagcggtc gtattgcgcc ataaacggca gcctcatcgc gaaaaacgag cccgccccca
     2161 aaatgagcat aggcaaggca taaatccata aaacggattg tgcgaacgaa cctttccatt
     2221 ttttaaacgg cttttcgatg cagtgataag aaatcaggga aagcagcaat atcaggacga
     2281 ttgctgccgc tgttgaataa ggcggcaggt tgtccgggcc gatatagcgc ataaaggcca
     2341 atatcggcca atgccacaga taaagcgaat aggaaatcaa accggcggca acagtgattt
     2401 tccattggaa aaatttttta agcgggtgtt cgtaatgatt gaaataaatc agcgcggcaa
     2461 cagccagaca gggaatcaaa gcggcgggtc ccgggaaata ggcggtttgt ttcgaatagg
     2521 caaacaggca ggcggacagt atgcacgcgg aaaacaatgc gccggcggcg gcacagcatc
     2581 tgccaacggc gagattccgc tgttgccggt accgcatcca cacggcggtc agcgatccga
     2641 ccagcatttc gcaggcgcgc aggtggggca ggtaatattt atcgaacgcg gaaggcataa
     2701 aggaagcggc aaggcttaag gcgcacaatg cggcgaggaa gccgaactgt acgcgcgggc
     2761 ttttgcgggc gacaagcagc agcaatatcg gaaagacaaa gtaaaattgt tcttcgaccg
     2821 acaaagacca aatgtgcagc aagggctttt cttcctgcgc gggatcgaaa taatccttcc
     2881 cccttgcaaa atacaggttg gaggcgaaac ccaaggcggt cagcgcggat ttccacaaaa
     2941 gaaagaaatc atctttggtg aataaaaaga agccgcctat cagcgttgcc gccaatacgg
     3001 cgaaaaatgc gggcaaaatc cgtttgatgc ggcggatata aaatgccttc agggaaaacc
     3061 tccccccccc cgacatttcg cggtgaagaa tcatcgtcat caaaaagcct gaaatcacga
     3121 aaaatatatc gacaccgaga aacccgcccg gcagccaatt tttttcgata tggaacacga
     3181 tgacggacaa gacggcggcg gcgcgcaatg tgtcgatgtc cgggcggtag ggtaaggctt
     3241 ggctcataat gtt
//
LOCUS       DAWKST010000189         3248 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.189, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000189 DAWKST010000000
VERSION     DAWKST010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.189"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            1058..3184
                     /locus_tag="WJL87_001878"
     CDS             1058..3184
                     /locus_tag="WJL87_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ7800277.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNAKVVLDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQHMTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGYTNQYGANK
                     VLKSGLDKRSYLAKIGTTFGDGDHRIVLSHMQDQHRGERFIREEFGWQTFTRPDNTLA
                     KNTPSYRETTQSNTNLEYTGKNLGFVEKLNANAYVLEKKRYSADDKDSGYAGNVEGPN
                     HTRITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNGEFKSKEKEQATKEKDE
                     KKVHSYSLTNPTKTDTGAYIEAIHEINGFTLTGGLRYDRFKVKTHDGKTVSSSNLNPS
                     FGVIWQPHEYWSFSASHNYASRSPRLYDALQTHGKRGIISIADGTKAERARNTEIGFN
                     YNDGTFAANGSYFWQTIKDALANPQNRHDSVAVREAVNAGYIKNHGYELGASYRTGGL
                     TAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYRFQNPNLEIGWRGRYV
                     QKAVGSILVAGQKNREGKLENVVRQGFGVNDVFANWKPLGKDTLNVNLSVNNVFNKFY
                     YPHSQRWNNTLPGVGRDVRLGVNYKF"
BASE COUNT          876 a          895 c          746 g          731 t
ORIGIN      
        1 caaaagtggg aatctagaaa tgaaaagcag caggaattta tcggaaatga ccgaaactga
       61 acggactgga ttcccgcttt cgcgggaatg acggcgacag ggttgctgtt atagtggatg
      121 aacaaaaacc agtacgtcgt tgcctcgcct tagctcaaag agaacgattc tctaaggtgc
      181 tcaagcacca agtgaatcgg ttccgtccta tccgtactgt ctgcggcttc gccgccttgt
      241 cctgatttcc gttcgttttc ggttattccc gataaattac cgccgtttct catcatttct
      301 ttaacccttc gtcattcccg cgcaggcggg aatctagttt ttttgagttc cagttgtttc
      361 tgataaattc ttgcagcttt gagttcctag attcccactt ttgcgggaag gacgcggaaa
      421 ggttgctttt cgtcattccc gcgcaggctt cgtcattccc gcgcaggcgg gaatccagtc
      481 tgttcggttt cggttttttc cgatagattc ctgccgcgtt aggggttcta gattcccgct
      541 ttcgcgggaa ggacgcggaa aggttgcttt tcgtcattcc cgcgcaggcc tcgtcattcc
      601 cgcacaggct tcgtcattcc cgcgcaggcg ggaatccagt ctgttcggtt tcagtcattt
      661 ccgatagatt cctgccgcgt taggggttct agattcccgc tttcgcggga atgacgcgga
      721 aaggttgctt ttcgtcattc ccgcatttgt taatccacta tatttccgcc gttttttata
      781 gtggactaac aaaaaccagt acagcgttgc ctcgccttgc cgtactatct gtactgtctg
      841 cggcttcgtc gccttatcct gatttttgtt agtccactat acatttccga caaaacctgt
      901 caacaaaaaa caacacttcg caaataaaaa cgataatcag ctttacacaa atcccccctc
      961 tcgttaatat aaacaaaaat aattattatt tttcttatcc tgccaaacct taacggtttg
     1021 gatttacttc ccttcataca ctcaaaagga cgaacaaatg aacgccccgt ttttccgtct
     1081 cagcctgctc tcgctcacac ttgcagcagg ttttgcccac gcggcagaaa ataatgccaa
     1141 ggtcgtactg gataccgtta ccgtaaaagg cgaccgccaa ggcagcaaaa tccgtaccaa
     1201 catcgttacg cttcaacaaa aagacgaaag caccgcaacc gatatgcgcg aactcttaaa
     1261 agaagagccg tccatcgatt tcggcggcgg caacggcacg tcccaacata tgaccttgcg
     1321 cggtatgggt cagaactctg tcgacatcaa ggtggacaac gcctattccg acagccaaat
     1381 cctttaccac caaggcagat ttattgtcga tcccgctttg gttaaagtcg tttccgtaca
     1441 aaaaggcgcg ggttccgcct ctgccggtat cggcgcgacc aacggcgcga ttatcgccaa
     1501 aaccgtcgat gcccaagacc tgctcaaagg cttggataaa aactggggcg tgcgcctcaa
     1561 cagcggcttt gctagcaacg aaggcgtaag ctacggcgca agcgtattcg gaaaagaggg
     1621 caacttcgac ggcttgttct cttacaaccg caacgatgaa aaagattacg aagcaggtaa
     1681 aggctacacc aaccaatacg gcgcaaacaa agtcttaaaa agcggattgg acaaacgcag
     1741 ctacctcgcc aaaatcggaa caaccttcgg cgatggtgat caccgcattg tcttaagcca
     1801 tatgcaggat caacaccgtg gcgaacgctt catccgagaa gaattcggtt ggcagacctt
     1861 tacccgtccg gacaacacgc tagctaaaaa tactccctca taccgcgaaa ccactcaatc
     1921 caacaccaat ttggagtaca cgggtaaaaa cctgggcttt gtcgaaaaac tgaacgccaa
     1981 cgcctatgta ttggaaaaga aacgttattc cgccgatgac aaagatagcg gctacgcagg
     2041 caatgtagaa ggccctaacc atacccgaat caccactcgg ggtatgaatt tcaactttga
     2101 cagccgcctt gccgaacaaa ccctgctgaa atacggtatc aactaccgcc atcaggaaat
     2161 caaaccgcaa gcgtttttga acggcgaatt taaaagtaaa gaaaaagaac aggcaactaa
     2221 ggaaaaggat gaaaagaaag tccattccta cagcctgacc aacccgacca aaacggatac
     2281 cggcgcgtat atcgaagcca ttcacgaaat taacggcttt accctgaccg gcgggctgcg
     2341 ttacgaccgc ttcaaggtaa aaacccacga cggcaaaacc gtttcaagca gcaaccttaa
     2401 cccgagtttc ggtgtgattt ggcagccgca cgaatactgg agcttcagcg cgagccacaa
     2461 ctacgccagc cgcagcccgc gcctgtatga cgcgctgcaa acccacggca aacgcggcat
     2521 catctcgatt gccgacggca cgaaagccga acgcgcacga aataccgaaa tcggcttcaa
     2581 ctacaacgac ggcacgtttg ccgcaaacgg cagctacttc tggcagacca tcaaagacgc
     2641 gcttgccaat ccgcaaaacc gccacgactc tgtcgccgtc cgtgaagccg tcaatgccgg
     2701 ttacatcaaa aaccacggtt acgaattggg cgcgtcctac cgcaccggcg gcctgactgc
     2761 caaagtcggc gtaagccaca gcaaaccgcg cttttacgat acgcacaaag acaagctgtt
     2821 gagcgcgaat cctgaatttg gcgcacaagt cggccgcact tggacggctt cccttgccta
     2881 ccgcttccaa aatccgaatc tggaaatcgg ctggcgcggc cgttatgttc aaaaagccgt
     2941 gggttcgata ttggtggcag gtcaaaaaaa ccgcgaaggc aaattggaaa acgttgtacg
     3001 ccaaggtttc ggtgtgaacg atgtcttcgc caactggaaa ccgctgggca aagacacgct
     3061 caatgtcaat ctttcggtta acaacgtgtt caacaagttc tactatccgc acagccaacg
     3121 ctggaacaat accctgccgg gcgtgggacg tgatgtgcgc ttgggcgtga actacaagtt
     3181 ctaaaacgca tatccagaaa aaatgccgtc tgaaagcctt tcaggcggca tctgttctga
     3241 taatttga
//
LOCUS       DAWKST010000190         3232 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.190, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000190 DAWKST010000000
VERSION     DAWKST010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.190"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(119..1219)
                     /locus_tag="WJL87_001879"
     CDS             complement(119..1219)
                     /locus_tag="WJL87_001879"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ7800278.1"
                     /translation="METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGADELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAERTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDNRE
                     TRAVLNIGGIANISVLPPDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFARPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVF
                     DAVSHAAADTRQMYICGGGIRNPVLMADLAECFGTRVSLHSTAELNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            1544..3118
                     /locus_tag="WJL87_001880"
     CDS             1544..3118
                     /locus_tag="WJL87_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ7800279.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
BASE COUNT          665 a          804 c          953 g          810 t
ORIGIN      
        1 gcggggtttg tttgatgttg aacggatttt gagtgtaaag ggattttaag ggtttgtaaa
       61 caaaaggggc gaaaatgccg tctgagcggc ggaaatggct ttcagacggt atttgcgctc
      121 aataataata tcccgcgccc agaatacacg gtttggatgc gccggttgct ttgtgcgggc
      181 tgccgggaat gcggttaatc caacacgccg ccaaccacgc aaatgcggct gcctccaccc
      241 attgaggatc gaggttcagt tcggcggtgc tgtgcaggga aacgcgtgtg ccgaaacatt
      301 ctgccaaatc cgccattaaa acaggattgc ggatgccgcc gccgcaaata tacatttgac
      361 gggtatctgc cgctgcgtgt gagacggcgt cgaaaacggt ttgcgcggta aaacgggaaa
      421 gcgtccgcaa tacgtcgtat cggttttcgc cgccgtcaag gtaggtttcg agccaattta
      481 gggcaaacag ttcgcgcccc gtgcttttgg ggtggggtcg tgcgaaatac gggtgggcga
      541 gcagcctgtc gagcagttgc ggcaatatgt tgccttgtgc cgcctttgca ccgtttttgt
      601 cgtaaggaag ctgccagtgt gcctgcgtcc acgcgtccat cagcatattg cccggccctg
      661 tgtcgaagcc gaaggcgggt gcgtcggggg ggagtacgct gatgttggca atcccgccga
      721 tgttcagtac cgcgcgtgtt tccctgttgt cgcggaacag ggcttcgtga aaggcgggga
      781 cgagtggcgc gccttgtccg ccggccgcaa ggtcgcggct gcggaagtcg ccgacggtaa
      841 aaatccgcgt ccgttccgcc agcagcggca aatcggcaag ctgtatgctg taaccgtgtt
      901 ccggcgcgtg tcggacggtt tgcccgtggc agccgagggc ggtaatgtcg gatggtgcga
      961 ggttttgact gcacagcagt tcggcggcgg tttgcgcgta caggcggctg agttcttgcg
     1021 acaaaatcct gctgcggtgc agttcgtctg cgcctgtgtc ctgcaaatcc agcaattggc
     1081 ggcgcagcct gtcgggatag ggggtaaagg cgtgcccttc cgcgcccagc catttgccgc
     1141 cgtccatccg tatcagtacg gcatccgccc cgtccatgct ggttcccgac atgatgccga
     1201 tgtaaagctg tgtttccatc atcactccca aactggtgca aaacgccatt ttaacgtgta
     1261 ttgacgctcg tataccgatt tgccgccgca gtgtaaataa agtgtaaata aatgtttcaa
     1321 gacggatgga aaaatattat aatgcgcccg caacatccag tagtagaagt gtcatacaaa
     1381 ccgtttccgg cagcagtttt gcattcggtc aggtttgggg gtatttggat gcggttcaga
     1441 aggaaggatg cgtctgccat atcccgaaac ggcagttcga ccggaggcag cagtacagtg
     1501 tcggcaacac tcatgatttc caccacatta aaggaagatt gccatggctc aaatccaaat
     1561 gagcgcaaat gttaaaacca tcaacgccgt ctttgccgcc atgctggtag gtacagtcgg
     1621 ctattttatt tattggggct tgggttatac ccattacaat tacgccgcct tattcattat
     1681 tgccacgatg ttcggcgtgt ttatggcgtt caacatcggc ggcaacgatg ttgccaattc
     1741 tttcggcacc agcgtcggtg cgggtacgct gaccatcccg caggctttgc tgattgcggc
     1801 ggtatttgag gtcagcggcg cggtcatcgc gggcggcgag gtaaccaata ccatacgcaa
     1861 aggcatcgtc gatttgaagg gtgttgattt cgaacccata cagtttgtgt ttattatgat
     1921 gtccgcgctt ttggcggcgg cgttgtggct gttgtttgcc tcgaaaaaag ggcttccggt
     1981 atctaccacc cattccatta tcggcggcat tgtcggcagc gcggtatgta tggcggtaat
     2041 gaacgatgcc gcatcgggcg atttgatacg ttggggcaag ctgggcggta ttggtgtttc
     2101 ttgggtattg tcgcccgtgt tgggcggcgc ggtgtcctat ttcctgtttt cgcgcgtcaa
     2161 gaaaaacgtc ttagattaca acgcttgggc ggaaggcacg ctcaagggca tcaagcagga
     2221 aaaaaaggcc tataaagaac ggcaccgcct gtttttcgag ggtttgtccg aagccgaaaa
     2281 agtcgagtac gccaccaaaa tggcgcacga cgcgcaaatt tacgacgaac ccgaattcga
     2341 tccgcaagag ctgcaatcgg agtattaccg cggtctttat gcgttcgaca accgtaaaaa
     2401 caatgtcgat tcctacaaag cactgcattc ttggattccc tttatcgctt cgttcggcgc
     2461 gatgatgatt tccgctatgc tgattttcaa gggcttgaaa aacctgcatt tggggatgag
     2521 caacgtcaac agcttcctga ccatctttat gataggcgcg gcggtgtgga tggggacgtt
     2581 tgtgtttgcc aaaagcctca agcgtaaaga cttgggcaaa tcgacctttc agatgttttc
     2641 atggatgcag gtctttaccg cctgcggctt cgcattcagc cacggggcga acgatatcgc
     2701 caacgccatc ggtccgtttg ccgcgattat ggatgttttg cgtaccaaca gcgttgccgc
     2761 gcaaaatgtc gtccccccga ttgcgatgct gactttcggc atcgcgctga ttgtcggttt
     2821 gtggtttgtc ggtaaagagg tgattaaaac cgtcggtacg agtttggcgg aaatgcaccc
     2881 cgcctcgggt tttaccgccg aactgtccgc cgcatccgtc gtgatgggcg cgtcgctgat
     2941 ggggctgccc gtgtccagta cgcatatctt ggtcggcgcg gtactcggca tcggtctggt
     3001 caaccgcaat gccaactgga aactgatgaa gcccatcggt ttggcgtggg tcattaccct
     3061 gcctgccgcc gccgtattgt cggttgtctg ctacttggtt ttacaggcag tattctgatt
     3121 gtaaaatact gatgccgtct gaacccgtgt tcagacggca ttttgttgat ggaatgtgcg
     3181 ggcttgtgcc ttatgcacaa tctgttctgt cgggatatgc cgtttggtat ag
//
LOCUS       DAWKST010000191         3141 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.191, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000191 DAWKST010000000
VERSION     DAWKST010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.191"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(311..787)
                     /gene="greA"
                     /locus_tag="WJL87_001881"
     CDS             complement(311..787)
                     /gene="greA"
                     /locus_tag="WJL87_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ7800280.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            1182..2483
                     /gene="aroA"
                     /locus_tag="WJL87_001882"
     CDS             1182..2483
                     /gene="aroA"
                     /locus_tag="WJL87_001882"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ7800281.1"
                     /translation="MTESVRLPVARLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVQIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLADALRIAGADVEYLGKEHYPPLHIGKRQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVAN
                     ENYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKERAVQSFDLDANHIPDAAMTLAIVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPKTLTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFDVFSSLTETTE"
     gene            complement(2526..3059)
                     /locus_tag="WJL87_001883"
     CDS             complement(2526..3059)
                     /locus_tag="WJL87_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ7800282.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGTHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          665 a          836 c          853 g          787 t
ORIGIN      
        1 attttaggtt tctgattttg gttttctgtt tttgagggaa tgacgggatg caggttttct
       61 taaccctgcg tcctagattc ccgcttttgc gggaatgacg gcgacagggt tgctgttata
      121 gtggatgaac aaaaaccagt acggggttgt ctcgccttag ctcaaagaga acgattctct
      181 aaggtgctga agcaccaagt gaatcggttt cgtactatct gtactgtctg cggcttcgtc
      241 gccttgtcct gatttttgtt aatccactat aatttcctgc gtgtgtcggg tgtatcgaaa
      301 tcaagccgaa tcaaatatat cggacttcga taatgtcgta ttcgcgcacg ccgcccgggg
      361 cttggacttc cgccgtatcg ccctcttcct tgccgattaa ggcgcgggca atcggtgagc
      421 cgacatagat tttgccctgt ttgatgtcgg cttcgtcttc gccgacaatt tgataggtaa
      481 cgtgttcttc cgtttccaaa tcttccagcg taaccgtcgt accgaacacg attttgcctt
      541 cggcgtggat ttcggtcgga ttgatgatgt gggcaacgga aagtttgtgt tccagctcgg
      601 aaatgcggcc ctcgataaag ccttggcgtt ctttggcggc ttcgtattcg gcgttttcgg
      661 acaaatcgcc gtgcgaacgg gcttcggcaa tcgcttcgat cacttcggga cgcgccacgc
      721 ttttgagctg ctgcaattcc tgtttcagca attccgcacc gcgtacggtc agggggattt
      781 tttgcatcgg tcttttttct ccatattccg gcacaccgtt ttgcggcagc aagcataccg
      841 cgtaccgtct tgttttgtgc gtccggatat taaaataaaa atacaagccg cccggaaaat
      901 cggcggcttg tctgtcgttg aacagcggct attctaccaa attctatgaa attggcaatc
      961 gtgccgtgcc gccgacaaac gcgccatgtc cgcaacaaaa gctgaaaata tgccgtcaaa
     1021 gaaattttag aaacaaaaaa tttaaaaata atcaattttc ggcataaaaa accacattta
     1081 cggactttaa aaccgaaaat gccaagcctg agatttttca tacagcattt gcaccagtat
     1141 aatgcaggct gtttttatct ttataatatt gacgttttgc catgaccgaa tccgtccgcc
     1201 tccccgtcgc ccgtctcaaa ccttccaccg tcgccctgcc cggctccaaa agcatcagca
     1261 accgcaccct gctgcttgcc gccttgtccg acaatgcttg cgaaatccat tccctgctca
     1321 aatccgacga taccgaccgt atgctcgaag cactcgataa actcggcgtt caaatcgaat
     1381 atcttgccga agaccgtctg aaagtgcacg gcacaggcgg acgcttcccc aaccgcactg
     1441 ccgatttgtt tttgggcaac gcgggcacgg cgttccgtcc gctgactgcc gcacttgccg
     1501 ttttgggcgg cgattatcat ctgcacggcg tgcctcgtat gcacgaacgc cccatcggcg
     1561 atttggccga tgcgctgcgg attgccggcg ccgatgtcga atatctcggc aaggaacact
     1621 atccgccgct tcatatcggc aaacgtcaag acaacggcga acgcgtgatt ccgattaaag
     1681 gcaatgtgtc cagccagttt ctgaccgccc ttttaatggc gttgccgctg accgggcagg
     1741 cgtttgaaat ccgtatggtc ggcgaattga tttccaagcc ctatatcgac attactttaa
     1801 aactgatggc gcaattcggc gtacaggttg ccaatgaaaa ctaccgcgtc ttcaaaattc
     1861 ccgccgatgc gcactaccac gcgcccgaac acttgcacgt cgaaggcgat gcctccagcg
     1921 cgtcctactt cctcgcagcc ggtttgattg ctgccacgcc cgtgcgcgtg accggcatcg
     1981 gcgcaaacag catacagggc gatgtcgcct ttgcccgcga gctggaaaaa atcggggcgg
     2041 acgtggtttg gggcgaaaac ttcgtcgaag tttcacgccc gaaggaacgt gccgtccaat
     2101 cctttgattt ggatgcgaac catatccccg atgccgccat gaccctcgcc atcgtcgcgc
     2161 ttgctacagg gcaaacctgc acgctgcgca acatcggttc gtggcgcgtc aaagaaaccg
     2221 accgcatcgc cgcgatggca aacgagttgc gcaaactcgg ggcaaaagtc gtcgaagaag
     2281 ccgaagcaat tcacatcacc ccgcccaaaa cgctgacacc cgacgccgtc atcgacacgt
     2341 acgacgacca ccgcatggcg atgtgtttct cgctggtttc gctgttgggc gtacccgtcg
     2401 tcatcaacga tccgaaatgc acccataaaa ccttcccgac ttatttcgac gtgttctctt
     2461 cactgaccga aacaacggaa taaggcggca ttttgccgcg attccggcgc ggcgcggcgg
     2521 gcggctcatt ctgtaaagaa agtatgtgcg ccgaggtagt ttttggcgta aaacggtgtg
     2581 gagagttttt cggttttgat ggttttgccg ctgctggggg catggatgaa ttcgccgttg
     2641 ccgatataga gtccgacgtg tgagtagcgg tgtgtgccgc cggtgttgaa gaatacgagg
     2701 tcgccggcct taaggcggct gtcggggatt ttgcggcttg ccgccgccat gtcgcgggcg
     2761 gtgcgcggca gcttgacgtt gagggcgttt ttgtaaacga attgaatcat gccgctgcaa
     2821 tcgaagccgg ttgcggtgct gctgccgccc catttgtagg gcgtgccgat gagtccgagg
     2881 ctgtggagca tgagttcctg cgagccttgt gtgcggtcga tgtggctgat gcggacggct
     2941 tggatttgcc ggactgtctg tttgggtttc ggttggcggt gtttgccgga ggtcgtgccg
     3001 catgaggcga ggagcagtgc gctgagacag aggaaaaggg ttttgtcggg gggaaacatg
     3061 gtttttcctt tgcgggttcg gatatccgtc tgaaggtgtt tcagacggta tagtggatta
     3121 acaaaaatca ggacaaggcg a
//
LOCUS       DAWKST010000192         3108 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.192, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000192 DAWKST010000000
VERSION     DAWKST010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.192"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            25..567
                     /locus_tag="WJL87_001884"
     CDS             25..567
                     /locus_tag="WJL87_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ7800283.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVATLLNNFYGQKLTEEDVL
                     KKLDKEQMPASFEDMRRIMPDLGFEAKGYALSFEQLAQLQIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKGRDAGGDKAFFT
                     RSPKRQTEFAVGQVKWWRAY"
     gene            697..2358
                     /locus_tag="WJL87_001885"
     CDS             697..2358
                     /locus_tag="WJL87_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ7800284.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENLGVEIYPGFAAAEVLYHKDGSVKGIATGNM
                     GVGKNGEPTDSFQPGMELWARQTLFAEGCRGSLSKQIIERFKLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGFQSLPKLSFKGGVLVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVENFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGSPRLQINAANCVHCKTCDIKDPTQNITWICPESASGPNYGGM"
     gene            complement(2492..2986)
                     /locus_tag="WJL87_001886"
     CDS             complement(2492..2986)
                     /locus_tag="WJL87_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ7800285.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDT
                     YIFK"
     gene            complement(2983..>3108)
                     /locus_tag="WJL87_001887"
     CDS             complement(2983..>3108)
                     /locus_tag="WJL87_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ7800286.1"
                     /translation="AGMAGLSGQISLTVPVLLTAQVLWVIIPLVLAAGIFRKRQI"
BASE COUNT          774 a          776 c          841 g          717 t
ORIGIN      
        1 cctgttgctt atggaaaaat caaagtgcag agttggaaag agaggcggga tttcaatatt
       61 gtaaagcagg atttggattt ttcctgtggg gcggcttcgg tggcgacgct tttgaacaat
      121 ttttacgggc aaaagctgac ggaagaggac gtgttaaaaa aactggataa ggagcagatg
      181 cccgcgtcgt ttgaggatat gcggcgcatt atgcctgatt tgggttttga agcgaaaggc
      241 tatgccctgt ctttcgagca gcttgcacag ttgcaaatcc ccgtcatcgt gtatctgaaa
      301 taccgcaagg atgatcattt ctcggtattg cgcggtatag acggcaatac ggttttgctt
      361 gccgacccgt cgctgggtca tgtttcaatg agcagggcgc agtttttgga tgcttggcaa
      421 acccgtgagg gaaatttggc aggtaagatt ttggcggttg tgccgaaagg gagggatgcc
      481 ggcggggata aggcgttttt cacacgcagt ccgaaacgtc agacggagtt tgcggtcgga
      541 caggtaaaat ggtggcgtgc ttattgaaat ttcgacaaag gtcgtctgaa aaccgaaaat
      601 atggttttca gacgaccttt gttgtatttg gtaactatat gttctcgttg tataattacg
      661 gatttgcaat tcaataataa atacacagga accgccatga cagaatccat cacccgcgac
      721 agtatgcaat acgatgtcgt gattgtcggc gcaggcccat cgggtttgtc cgccgccatc
      781 aaactcaagc agcttgccga acaaaatggg cgcgaaatca gcgtttgcgt ggtggagaaa
      841 ggttcggagg cgggtgcaca ttcgctggcg ggcgcggtta tcgatccgat tgcgctgaat
      901 gagttgattc ccgactggaa agaaaaaggc gcgccgctga cgcgtacggt aacgcaggac
      961 aaagtgttgt tcctgactga gaaaaaagcg ttcaatctgc ctattacccc aaatttcgac
     1021 aatcacggca actacatcgt cagcttgggc gaagtcgtgc gctggttggc ggagcaggcg
     1081 gaaaatttgg gcgtggaaat ttatccgggc tttgccgctg ccgaagtgct gtatcacaaa
     1141 gacggttcgg tcaaaggcat tgcaaccggc aatatgggcg tgggcaaaaa cggcgagccg
     1201 accgattcat tccagcccgg catggagctt tgggcacggc aaaccctgtt tgccgagggc
     1261 tgccgaggtt cgctttccaa acaaatcatc gaacgcttca aactcgacca aaacagccag
     1321 ccgcaaactt acggcttggg cattaaagaa gtttgggaag tgccgtccga acagcatcag
     1381 cccggtttgg tggtgcacag cgcaggctgg ccgctggaca gcaaaaccta cggcggcgcg
     1441 tttgtttacc atttcgacga caacaaagtt gccgtcggct tcgtggtcgg tttggactat
     1501 caaaatcctt atctgtcgcc gtttgaagag ttccaacgtt tcaaaaccca tcccgaaatc
     1561 cgcaaaacct ttgaaggcgg ccgccgcatt gcttacggtg cgcgttcgct gattgaaggc
     1621 ggttttcaaa gtttgccgaa gctctcgttc aaaggcggcg ttttggtcgg cgatgcggcg
     1681 ggtttcctca atatgccgcg catcaaaggc attcataccg cgatgaaatc cgccatgctc
     1741 gccgccgaag ccgtgttccc cttgttggaa aaccttgaag aagtggaaaa cttcgacagc
     1801 ggcaaagagg cggcggatta tcagcaacgt tttgaacaaa gctggctgta tcaagagctt
     1861 tacgccgcgc gcaatgtccg tccgtcattc aaatggggcg tttacctcgg ctcaatctac
     1921 accggcatcg atcagatgat tttcagaggc aaagccccgt ggaccttgaa acatcacggc
     1981 aaagacaacg agcagcttaa aaaagccgcc gcgtgcaagc cgattgatta tccgaaaccc
     2041 gacggcgtat tgaccttcga ccgcttgagc agcgtcttcc ttgccaatct cgcgcacgaa
     2101 gaaaaccagc ccgaccattt ggtgctgaac aatccgcaaa cgatgataga cgtgaactac
     2161 aaagaatacg cctcacccga aacgcgctat tgtccggccg gcgtgtatga aatcgtcgaa
     2221 gaaaacggca gcccgcgcct gcaaatcaac gcggcaaact gcgtgcactg caaaacctgc
     2281 gacatcaaag acccgacgca aaacatcact tggatttgcc ccgaaagcgc gagcgggccg
     2341 aattacggcg ggatgtaaac cttattccgc cgttacgccc ggtcaggaat aaggaatggg
     2401 gcagttgttc aaacctgccc cgtttgaacc tatgacaaaa aatgccgtct gaaccttggc
     2461 ttcaacgttc agacggcatt tttgtttgga tttacttgaa aatataggta tcaggcatat
     2521 cgtcgaaacc gacaacctta ccgcctttat cctttgcaaa tttctcagcc tgctctttgt
     2581 tgccgaacgg cagcgcgtct tccgcaccca taccgccgat aaagccgctg tcgatgacgt
     2641 aaaaggcttt tttcgcatcc atccactccg tgtcggcatt gggattcgtc caatcggtaa
     2701 cattgcccat atcggtaacg taaatcacgc ggatgccttt gggttcttcg ggcagcttgg
     2761 tatagccgaa catctgcttg atggtggaga accaaacggg ctgatcgggt ttgccgttca
     2821 agaaaatctg ggctttgggg ccgttgtgtt cggtcaggtt catactgcaa tagtgtccga
     2881 ccgaacggtc gctaatctgc cggggtaaag gcggcggccc ctcttccgcc tgccggcagg
     2941 cacttaaggc ggaaacggca acaattgcca acagggtttt cttcatattt gtcgctttct
     3001 aaaaattccg gctgccaaaa caagcggaat gataacccat aaaacctgag cggtcagcaa
     3061 aacggggacg gtcaggctga tttgtccgct caaacccgcc atacccgc
//
LOCUS       DAWKST010000193         3106 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.193, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000193 DAWKST010000000
VERSION     DAWKST010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.193"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..211)
                     /locus_tag="WJL87_001888"
     CDS             complement(<1..211)
                     /locus_tag="WJL87_001888"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800287.1"
                     /translation="MLPTRKTARKIQARHIALPFSGKPQFYRPQERLSKQAQATPPGH
                     FRFHRLSVVRIMQQHHQRITLFGRQQ"
     gene            271..894
                     /locus_tag="WJL87_001889"
     CDS             271..894
                     /locus_tag="WJL87_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ7800288.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1005..2387
                     /locus_tag="WJL87_001890"
     CDS             1005..2387
                     /locus_tag="WJL87_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7800289.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLIAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFLLTGILTVASIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNQEGLYRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2391..2915
                     /locus_tag="WJL87_001891"
     CDS             2391..2915
                     /locus_tag="WJL87_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ7800290.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          622 a          840 c          938 g          706 t
ORIGIN      
        1 cctgctgccg cccgaaaagc gtgatgcgct gatggtgctg ctgcataatc cggacgacgg
       61 ataaccggtg aaaacggaaa tgcccgggcg gtgttgcctg tgcctgtttg gacaagcgtt
      121 cttgagggcg gtagaattga ggtttgcccg aaaagggcag ggcgatatgc cgcgcttgga
      181 ttttgcgcgc ggtttttcgc gttggaagca atcattgtct gtttgaatat ttaatgccgt
      241 ctgaaacgtc cggacgcgcc cccgttccct atgaaaaaac cgaccgatac cctacccgtt
      301 aatctgcaac gccgccgcct gttgtgtgcc gccggtgcgt tgttgctcag tcctctggcg
      361 cacgccggcg cgcaacgtga ggaaacgctt gccgacgatg tggcttccgt gatgaggagt
      421 tctgtcggca gcgtcaatcc gccgaggctg gtgtttgaca atccgaaaga gggcgaacgt
      481 tggttgtctg ccatgtcggc acgtttggca aggttcgtcc ccgaggagga ggagcggcgc
      541 aggctgctgg tcaatatcca gtacgaaagc agccgggccg gtttggatac gcagattgtg
      601 ttggggctga ttgaggtgga aagcgcgttc cgccagtatg caatcagcgg tgtcggcgcg
      661 cgcggcctga tgcaggttat gccgttttgg aaaaactaca tcggcaaacc ggcgcacaac
      721 ctgttcgaca tccgcaccaa cctgcgttac ggctgtacca tcctgcgcca ttaccggaat
      781 cttgaaaaag gcaacatcgt ccgcgcgctt gcccgcttta acggcagctt gggcagcaat
      841 aaatatccga acgccgtttt gggcgcgtgg cgcaaccgct ggcagtggcg ttgattttga
      901 acccgcgccg caaccgaaat acggcgaatc ctgtataatc cgaaaatctg ttcactggaa
      961 gttcagacgg cattgcaact gttgatgccg tctgaaaaaa tatgatggca agagacaacc
     1021 gcatccaaat gtttccgcac gaatggcgcg ccagtacgac gctttccggc gtgtacgcgc
     1081 tgcgtatgct gggtatgttc ctcgtgctgc ccgtattggc ggtgtatgcc gcctcgctgc
     1141 ccggcgcgga aggcaacaaa acgctggtcg ggctggcaat gggcatttac gggctgacac
     1201 aggctctgct gcaactgcct ttgggcatcg cttccgacaa gttcgggcgc aagaaaacca
     1261 tttatgtcgg gctgattgtg tttgcagcgg gcagcttcct tgccgccgcc gccgatacgc
     1321 tgcccatgct gatcgccgca cgcgccatac agggtgcggg agcggttagt gcggcggtta
     1381 ccgcgctgct ggcggatttg acgcgcgacg gcgtacgtac ccgcgcaatg gcgatgatcg
     1441 gtttgagtat cggtctgacg ttttcggtca gcctcgtcgt tgcccctgtg attgccgacg
     1501 cggtgggcgt acgcggcttg tttctgctga ccggtatttt gaccgttgcc agtatcggcg
     1561 tggtggcgtg gatgactccc gacccggagg tttccaagct gcacgaagat acgcaggcgc
     1621 agccttcgcg cataggcgaa gttttgaaaa accgtaggct gctgacgctc gatttcggca
     1681 ttttcgccct gcacgccgcg caaatggcat tgtttaccgc gctgcctttc gcgatgaccc
     1741 agctcggttt ggaaaaaata cagcattgga aagtctatct gccctcgacc attacgggct
     1801 tggtggtgat ggttccgctg attatcgtcg gcgagacgcg caacaagctc aaacaggttt
     1861 ttgttttggg tatcgtctgt attgcggcgg cgcagctcgg tttgctgtcc ggtatgcgct
     1921 cggtaggctt gattaccgct tatttggttg tttactttat cggttttaat gtgttggaag
     1981 cgagcctgcc gtctatggtt tccaaaatcg cgccgtccga cctgaagggt acggcgatgg
     2041 gcgtgtacaa cacgatgcag tcgctcggac tgtttgccgg cggcgcggca ggcggtttgc
     2101 tgtttcaaaa atacggcttt tccggcgtgt ttgccttttg cagtatattg atgctgctgt
     2161 ggctggtaat tgccgtttta tcgcctgcgc ccaagcccgt caaaaacctc agttaccctg
     2221 tcggcggcgt gtggcagggc aatcaggaag ggttataccg cgccttgtcg gagcttgagg
     2281 gtgtggaaga catcggtttc agtttcgacg ggcagaccgt ctatctcaaa gtgttgcaga
     2341 agggtttcga tcaggctgcc gctgaaaaaa tcatcacagg agtttaaaaa atgtcattga
     2401 acaaagtcat cctcatcggc cgcctcggac gcgatcccga agtgcgctat atgcccaacg
     2461 gcgaggcggt ttgtaatttc agcgtcgcca ccagcgaaac ttggaacgac cgcaacggcc
     2521 aacgtgtaga gcgtactgag tggcacaaca tcaccatgta ccgcaaactg gcggaaattg
     2581 ccgggcaata cctcaaaaaa ggcgggctgg tttatctgga aggcagaatc caaagccgca
     2641 aataccaagg caaagacggc atcgaacgca ccgcttacga tattgtcgcc aacgaaatga
     2701 aaatgttggg cgggcgcaat gaaaacagcg gcggtgcgcc ttacgaggaa ggttacggtc
     2761 agagtcagga ggcttaccaa cgccccgcgc agcaaagccg gcagcccgcc tccgacgcgc
     2821 cgtcccatcc ccaagaagca ccagccgcgc cgcgccgcca acccgtgcct gccgccgccc
     2881 cggtcgagga cattgacgac gatatcccgt tctgaatttt acggccggac atcctcgcgg
     2941 agggaaatca taaaggacgg agaaacctta aaccttacgg ggcagggttt ctcttttttg
     3001 catttgggcc ggcttgttgc cgccctttgc gttgggcgga acgttgcgat tgcgccgttc
     3061 gggcgttttg aggcagtgat gcctgccgca cgtccccgct gttttc
//
LOCUS       DAWKST010000194         3084 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.194, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000194 DAWKST010000000
VERSION     DAWKST010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.194"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..260)
                     /locus_tag="WJL87_001892"
     CDS             complement(<1..260)
                     /locus_tag="WJL87_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800291.1"
                     /translation="MNMENYFSNLNLDIRTHKLGTFTDQKVTPDVLCAVAECIIEYIE
                     NIGEIFSINDIRYSDYAEYIATAVFKKPSIENAGSEYNKFFS"
     gene            complement(272..2860)
                     /gene="dcm"
                     /locus_tag="WJL87_001893"
     CDS             complement(272..2860)
                     /gene="dcm"
                     /locus_tag="WJL87_001893"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ7800292.1"
                     /translation="MKNQYSKSLHGVFYLAFYDKIHAFFAEQLFQLIINMNKKPTYIS
                     LFSSAGVGCYGFKTNGFECVATNELLEKRLKIQKYNQKCRFDSGYIAGDITLPETQAK
                     LFAEIRRWNTEIDVVIATPPCQGMSVANHKKNNEMARNSLVVESIKIVREVQPKFFIF
                     ENVKAFLKTPCTDTDYQEKPIEEAIWSNLGGHYNILSNVLNFKDYGANSSRTRTLVIG
                     VRKDIHHITPYDIFPKQTPPKTLRTLLADLPPLMQMGEISDDDIYHSYREFDRRMLPW
                     IAQLKEGESAFQNTDSARIPHQIKNGEIIYNQNKNGDKYARWFLDREGPCIHTRNDIL
                     ASQNTIHPTENRVFSVRELMRMMSIPPSFKWTAQDEKQLNALSFDEKKTFLKKEELNI
                     RHCIGESVPTAVLANVAQNIQAALQQSELSMTEIQNLIKRENLSLADNLYRFIEQHFD
                     AYPFSRLLQIAEYANAARSETAAYFTRIDIAFGLVNALPDFKKKKNLRILEPSVGIGN
                     FIPLLAKKYADKEKVIFDLVDIDSNSLNLLSLLLKKLNLGSKFEFNIIHQDFLTATLP
                     ENYDLVIGNPPYGKVKASDKNLALYRKNATNKDTQNIFSFFIDKALTLSKTVALVVPK
                     SVINAPEFQITRQQLITANINRIIDYGEKAFKGVKIETIAFIADQTDKDNQHITIESH
                     IQETLIQNCKNYFFSDNFPYWLIYRNSEFDQIAQKLEFNVFQSFRDRQITKAHTQNSG
                     NVRVLKSRNIGNNEIIDIEGYDSYVDSVAPFSVGKFINRKNVVMIPNLTYKPRAAFLP
                     ENSIADGSVALLTMKKHEKILTQRDLAYYATPEFEQFYRVARNYGSRSMNIDNHSVFF
                     FGLLKD"
BASE COUNT          899 a          570 c          607 g         1008 t
ORIGIN      
        1 tgtgaaaaaa atttattgta ttcgctaccg gcattttcaa tggatggttt tttaaatacg
       61 gcggtggcga tatattcggc ataatctgaa taacgaatat cgttaatgct aaatatttct
      121 ccaatatttt caatatattc gatgatacat tctgctacgg cacataatac atccggggtt
      181 actttttgat cggtaaatgt gccgagttta tgtgttctga tgtctaaatt taagttagaa
      241 aaataatttt ccatattcat tttttactcc tctaatcttt tagcaatccg aaaaaaaaca
      301 cagaatgatt gtctatattc attgaacgag aaccataatt acgggcaaca cggtaaaact
      361 gctcaaattc gggagttgca tagtatgcga gatctcgttg ggttaggatt ttttcgtgtt
      421 ttttcattgt cagtagggca accgagccat cagcaatact gttttcaggc aaaaatgctg
      481 cccgaggttt gtaagtcaga ttgggaatca tcacgacatt tttacggtta ataaatttgc
      541 cgacagaaaa aggggcaact gaatccacat aactatcata accctcaata tcaataatct
      601 cgttgttacc gatattacgg gattttaaaa cccgaacatt gccactattc tgtgtatgcg
      661 ctttggtaat ttggcgatct cggaatgatt gaaatacatt aaattccaat ttttgtgcaa
      721 tttgatcaaa ttcactatta cgataaatca accaataggg aaaattatca gaaaaaaaat
      781 agtttttaca gttttgtatc aacgtttctt ggatatggct ttctatcgta atatgttgat
      841 tgtctttgtc ggtttgatcg gcaataaagg caatcgtctc tatttttacg cctttaaatg
      901 ctttctcgcc gtaatcaata atgcggttga tattggctgt gataagttgt tgtcgggtta
      961 tttgaaactc gggggcgtta atcacacttt tgggaacaac caaagccacg gttttactga
     1021 gcgtcagagc cttatcaata aaaaatgaaa aaatattttg cgtatcttta ttggtagcat
     1081 ttttacggta tagtgccaaa tttttatcac ttgccttaac tttgccgtat ggtggattgc
     1141 cgatgactag atcatagttt tcaggcagcg ttgcggttaa aaaatcctga tgaataatat
     1201 tgaactcaaa tttactgcct aaatttaatt ttttcagcag caatgacagg agatttaaac
     1261 tgtttgagtc aatatccacc aaatcaaaaa taactttttc tttgtctgca tatttttttg
     1321 ccagtagtgg aatgaaatta ccaatgccga cagagggttc taaaatccgt aagttttttt
     1381 tctttttgaa atcaggaaga gcattaacca gtccgaaagc aatatcaata cgggtaaaat
     1441 aagcagccgt ttcgctacgg gcagcattag cgtattcagc aatttgcaaa agccgactga
     1501 atggatatgc gtcaaaatgt tgctcaataa atcggtagag attatcggca agggataaat
     1561 tttctcgttt gattaaattt tgaatttccg tcattgacag ttcggactgt tgcaaggctg
     1621 cctgaatgtt ttgtgcgaca ttggccaaaa cagcggttgg cacagattca ccgatacaat
     1681 ggcggatatt cagctcttct tttttcaaaa aggttttttt ctcatcaaat gacagtgcat
     1741 taagctgttt ttcatcttgt gccgtccatt taaatgatgg cggaattgac atcatacgca
     1801 tgagttcacg cacggaaaat acacggtttt cagtgggatg gatggtgttt tggctggcta
     1861 aaatgtcatt acgggtatgg atgcacggac cctcacgatc taaaaaccaa cgggcgtatt
     1921 tgtctccatt tttgttttga ttgtagataa tttcaccatt tttaatttga tggggaatgc
     1981 gtgcagaatc agtattttgg aaagctgact cgccttcttt aagctgtgca atccacggca
     2041 acatacggcg atcaaattca cgatagctat ggtaaatatc atcatcgcta atttcgccca
     2101 tttgcattaa aggcggcaga tcagccagca atgtacgcaa ggtttttggc ggtgtttgct
     2161 tggggaaaat atcgtacggc gtaatgtgat gaatatcttt tcgcacgcca atcaccagcg
     2221 ttctggtacg actggaattt gcaccataat ctttaaaatt tagtacattg gatagaatgt
     2281 tgtaatgtcc gcccaaattt gaccaaatgg cttcctcaat cggcttttct tgataatcgg
     2341 tatcggtgca gggtgttttt aaaaaggctt tgacgttttc aaaaataaaa aacttgggct
     2401 gaacttcccg aacgattttg attgattcca cgaccaaaga atttcttgcc atttcattat
     2461 tttttttatg gttggcaacc gacattcctt ggcaaggcgg tgtggcaatc acaacatcaa
     2521 tttcagtatt ccagcgtcga atttcggcaa acaatttggc ttgggtttca ggcagggtaa
     2581 tatcgccggc aatataacct gaatcaaagc ggcatttttg attatatttt tggattttta
     2641 ggcgtttttc taaaagttcg ttggttgcta cgcattcaaa accattggtt ttaaaaccat
     2701 agcaaccaac gcctgcactg ctaaataaac tgatgtaggt gggttttttg ttcatattta
     2761 tgatgagttg aaacaactgc tctgcaaaaa atgcgtgtat tttatcatag aaggcaaggt
     2821 aaaacacgcc atgcaatgat ttactatatt gatttttcat caaaaaagtg ctgtttgaac
     2881 agatatacag cggtgttttt tgcaatgcgt ttcaaacttg aggtacgttt ttaatagaaa
     2941 caccgtctga acgccaacct gagacctttg caaaattccc acaaatcccc caattcccac
     3001 caagacattt aggggatttc tcatgagcac tttcttccgg caaaccgccc aagccatgat
     3061 tgccaaacac atcgaccgct tccc
//
LOCUS       DAWKST010000195         2967 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.195, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000195 DAWKST010000000
VERSION     DAWKST010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.195"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            121..1665
                     /gene="purF"
                     /locus_tag="WJL87_001894"
     CDS             121..1665
                     /gene="purF"
                     /locus_tag="WJL87_001894"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ7800293.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIVTAEGGTFHM
                     HKGKGMVREVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTDSEGRKSYAVASESVA
                     FNALTYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANERSAAEIAAEIGADGIVFQNLSDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            1765..2256
                     /gene="greB"
                     /locus_tag="WJL87_001895"
     CDS             1765..2256
                     /gene="greB"
                     /locus_tag="WJL87_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ7800294.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            2315..2941
                     /locus_tag="WJL87_001896"
     CDS             2315..2941
                     /locus_tag="WJL87_001896"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ7800295.1"
                     /translation="MRKIRTKICGITTPEDASAAVAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDTFCRQFDRPYIK
                     AIRVQTASDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
BASE COUNT          713 a          908 c          775 g          571 t
ORIGIN      
        1 tattctccat aaacccacct atctgtcctt cgctcaaata ttgtcaacaa ttcaggcaaa
       61 aaaccaccgt ttgctttata attccgtttt acccttaaac ctcgaatcgg agaagacgat
      121 atgtgcggcg tattaggttt ggtcagtcat gaacccgtga accagcttct gtacgacggt
      181 ttgcagatgt tgcagcacag gggacaggat gcggcgggca ttgtaacggc cgaaggcggt
      241 accttccata tgcacaaagg caaagggatg gtgcgcgaag tgttccgcac gcgcaatatg
      301 cgtgatttga ccggcaacgc cggcatcgcc cacgtccgtt atcccacggc gggcaacgcc
      361 ggcagcagcg cggaggcgca gcctttctac gtcagctcgc cgttcggcat cgttttggcg
      421 cacaacggca acctcaccaa cactgccgaa ctttatgaaa acgtgtgcaa caaacaccta
      481 cgccacgtca acaccagctc cgattccgaa gtcttgctca acgtattcgc gcacgaattg
      541 cgccgcgaag tctctaaaaa cgccgacccg caccggctca atgccgacaa tattttcaac
      601 gccgttgccg aagtccaccg cctggtgcgc ggcgcatacg gcgtggttgc catgattgcg
      661 ggctacggta tgcttgcctt ccgcgaccct tacggcatcc gcccgctggt attgggttcg
      721 caaaccgaca gcgaaggcag aaaatcctat gccgtcgcct ccgaatccgt cgccttcaac
      781 gcgcttacct acgatttgga acgcgacatc cggccgggcg aagcggtatt tgtcggcttt
      841 gacggcacaa tgattgcccg ccaatgcagc gaccgtgcca aactcagccc ctgccttttt
      901 gaatatgttt attttgcccg ccccgactct gtgattgacg gcgtatccgt ttaccaatcg
      961 cgcttggata tgggcgtgtc gctggcggaa aaaatcaaac gcgagctgcc cgtggacggc
     1021 atcgatgtcg tgatgcccat ccccgacacc agccgcccca gcgcgatgga gcttgccgtc
     1081 catctcgaca aaccctaccg cgagggtttg attaaaaacc gctatatcgg ccgcaccttt
     1141 attatgcccg gacaggcgac gcgcaaaaaa tccgtgcgcc aaaaactcag cccgatggaa
     1201 accgaatttg caggcaaaag cgtgttgctg gtggacgact ccatcgtgcg cgggacgacc
     1261 agccgcgaaa tcgtcgaaat ggtacgcgcg gcgggcgcgc gcaaagtcta tatcgcctcc
     1321 gccgcgcccg aagtgcgcta tcccaatgtg tacggcattg atatgcccac gcgcgaagag
     1381 ttgattgcca acgagcgcag cgcggcggaa atcgccgccg aaatcggcgc agacggcatc
     1441 gtgttccaaa atttgagcga tttggaagcc gtcgtcaaag cactcaatcc gaaaatcgaa
     1501 tccttcgatt cgtcctgttt caacggcatt tatcagaccg gcgacatcga tgatgcctac
     1561 ctcgaccgcc tgtctgccga gaaatccggc tgcggcggtc tgaaaatcca cccgagcagg
     1621 atggaacaca gcatcagcat cagcgatgcg ggcgacgaag aataaggctt cccaaaccgc
     1681 gatgccgtct gaaatccgaa cgccgggttt tcagacggca tttcccatat cggcatcaca
     1741 tttcccgttt catccgcaca caatatgagt accgaaacca aaaactacat tacgcccgcc
     1801 ggatggcagg cattgaaaga cgagctttat caactggtca acaaagaacg ccccgaaatc
     1861 gtccaaatcg tcaactgggc ggcaggcaac ggcgaccgca gcgaaaacgg cgactatctt
     1921 tacggcaaac gccgtatgcg cgaaatcgac cgccgcatcc gtttcctgac caagcgtttg
     1981 gagtccgccg tcgtcgtcga tcccgaattg cgcgaagcga ccgaccaagt atttttcggc
     2041 gcgaccgtcg gcctgttgcg cggcgacggg cgcgagcaaa ccgtcaaaat cgtcggcatc
     2101 gatgaaatcg ataccgcgca aaacaaaatt tcttggattt cgccgttggc gcgttgcctg
     2161 attaaagcgc gcgaaggcga cgaagtggtt ttgaacacgc cggaagggcg tgaggaaatt
     2221 gaaatccttt ctgtcgaata catcaaaatc gactgatcgg gaatgccgtc tgaaacggtg
     2281 caaacgcatc gtttcccaaa taaaggaata ccgaatgagg aaaatccgca ccaaaatctg
     2341 cggcatcacc acaccggaag acgcatcggc cgccgtagcg gcaggtgcag atgccgtcgg
     2401 gctggtcttt ttccaaggca gcagccgggc cgtcgatatt gcccgcgcca aaaaaatcac
     2461 cgccgcactg ccgccgtttg tcagcgttgt cgcccttttc gtcaacgaaa gcgcgcaaaa
     2521 catccgccgc atccttgccg aagtgccgat acacatcatc caattccacg gcgacgaaga
     2581 cgacacattc tgccgccagt tcgaccgccc ctatatcaag gccattcgtg ttcagacggc
     2641 atcagacatc cgaaacgccg ccgaccgctt ccccgacgct caggcactgc tgttcgatgc
     2701 ctaccaccct tcggaatacg gcggcaccgg acaccgcttc gactggacgc tgctggcgga
     2761 atattcgggc aaaccgtggg tgcttgccgg cgggctgacc cctgaaaacg tcggcgaagc
     2821 cgtccgcatc accggagcgg aagcggtcga tgtatccggc ggcgtggaag cgtctaaagg
     2881 caaaaaagac ccagccaaag tcgccgcctt tatcgcaacc gccaaccgcc tatcccgtta
     2941 aagcaacaaa aattgccgcc ggaatga
//
LOCUS       DAWKST010000196         2834 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.196, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000196 DAWKST010000000
VERSION     DAWKST010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.196"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..316
                     /locus_tag="WJL87_001897"
     CDS             <1..316
                     /locus_tag="WJL87_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ7800296.1"
                     /translation="PTVPSKYTNVKKSSVRIGKHAILGTNVTVLPGVTIGDGVAVGAG
                     AIVRKNLEPWTIYVGVDCKPLKRRSQDKILELEKHLVAEFNLDLCPIIPSPNLPKPKE
                     TR"
     gene            313..876
                     /gene="gmhB"
                     /locus_tag="WJL87_001898"
     CDS             313..876
                     /gene="gmhB"
                     /locus_tag="WJL87_001898"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ7800297.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENTAPQAD"
     gene            905..1648
                     /locus_tag="WJL87_001899"
     CDS             905..1648
                     /locus_tag="WJL87_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ7800298.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     GKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            1645..2337
                     /locus_tag="WJL87_001900"
     CDS             1645..2337
                     /locus_tag="WJL87_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ7800299.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHGRQLALTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPASSSLTSAKTFWGV
                     CRKTTGIRFNWRLVGAPEYVADYVCIHELCHLAHADHSRAFWALTRRFAPYTPEAKQW
                     LKIHGRELFALG"
     gene            2448..2633
                     /locus_tag="WJL87_001901"
     CDS             2448..2633
                     /locus_tag="WJL87_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800300.1"
                     /translation="MPEQARARPIQTAMTLCRRFGAGWRHTRRLPRFISHRPSETRNP
                     PGPTCGMKRLSWREVFY"
BASE COUNT          710 a          897 c          681 g          546 t
ORIGIN      
        1 tccaacagtc ccatctaaat atactaatgt taaaaaatct agtgttagaa ttggaaaaca
       61 tgcaatttta ggaacaaatg taacagtatt accaggcgtt actattggtg atggggttgc
      121 tgttggagca ggggctatag tgagaaaaaa tcttgagccg tggactattt atgtcggggt
      181 agattgtaaa ccactgaagc gcagatcaca agataaaata ttggagttag agaaacatct
      241 tgtagcagaa tttaatctag atttatgtcc tataatccca tccccgaacc tgcccaaacc
      301 aaaggaaacg cgatgaaact catcattctc gaccgcgacg gcgtcatcaa tcaggaccgc
      361 gacgacttcg tcaaatccgt tgacgagtgg atacctgtcg aaggcagcat ggatgcggtg
      421 gcatttctga cgcaggcagg ctacaccgtc gccgttgcca ccaaccaatc cggcatcggg
      481 cgcaaatatt ttaccgttca aaaccttacc gaaatgcacg ccaaaatgca ccgcctcgtc
      541 cgtcaggcag gcggcgaaat caacggcatc tggttctgcc cgcacaccga tgccgacaac
      601 tgcaactgcc gcaagcccaa accgggtatg attgaagaca tcatcggacg cttcaacgcc
      661 caagcctcgg aaacctggct ggtcggcgac agcctgcgcg atttgcaggc aatcgatgcc
      721 gtcggcggaa aacccgcgct ggttctgacc ggaaaaggca aaaaaacgct ctcccaacac
      781 ggacacgaat tacccgaaca cacacaggtt ttcgatacct tgctcgattt ctcacaatac
      841 atcatgcagg aaaacaccgc accgcaagcc gactgaacat accgcattcc gacaaggcaa
      901 aaccatgctc atcatccgca acctgattta ctggctgata ctctgttcca ccctgatttt
      961 cctctttccc tttatgctgc tcgcctcgcc tttccgagac ggggcgcaca agatggcgcg
     1021 ggtctgggtc aaaatcctca acctctcgct caaacacatc gtcgggctca aataccgcat
     1081 catcggcgcg gaaaacatcc ccgaccgccc cgccgtcatc tgcgccaaac accaaagcgg
     1141 ctgggaaacg ctcgcccttc aggacatttt tccgccgcag gtttacgttg ccaaacgcga
     1201 gttgttcaaa atcccctttt tcggctgggg cttgaaactg gtcaaaacca taggcataga
     1261 ccgcaacaac cgccgcgaag ccaacgagca gctcataaaa caggggttgg cgcgcaaaaa
     1321 cgaaggctat tggattacca ttttccccga aggcacgcgc cttgcgcctg gaaaacgcgg
     1381 caaatacaaa ctcggcggcg cgcgcatggc gaaaatgttt gagatggaca tcgtccccgt
     1441 cgccctcaac agcggcgaat tttggccgaa aaactccttt ctgaaatatc cgggggaaat
     1501 caccgtcgtc atctgtccga ccatcccgca cgcaagcggc agcgaagccg aattgatggg
     1561 aaaatgcgaa cacctcatcg aaacgcagca gccgctcatt tccggcgcag gcccgtttgc
     1621 cgccaaaatg ccgtctgaaa ccgcatgacc gcctttgtcc acaccctttc agacggcatg
     1681 gaactgaccg tcgaaatcaa gcgccgtgcc aagaaaaacc tgattatccg ccccgccggc
     1741 acacataccg tccgcatcag cgtcccaccc tgcttctccg tctccgctct aaaccgctgg
     1801 ctgtatgaaa acgaagccgt cctgcggcaa acactggcga aaacaccgcc gccgcaaact
     1861 gccgaaaacc ggctgcccga atccatcctc ttccacggca gacagcttgc cctcaccgcc
     1921 catcaagaca cgcaaatcct gctgatgccg tctgaaatcc gtgttcccga aggcgcaccc
     1981 gaaaaacagc ttgcgctgct gcgggacttt ttggaacggc aggcgcacag ttacctgatt
     2041 ccccgcctcg aacgccacgc ccgcaccaca caactgttcc ccgcctcctc ctcgctgacc
     2101 tctgccaaaa ccttctgggg cgtgtgccgc aaaaccacag gcatacgctt caactggcgg
     2161 ctggtcggcg caccggaata cgttgccgat tatgtctgca tacacgaact ctgccacctc
     2221 gcccacgccg accacagccg cgcgttctgg gcactgaccc gccgcttcgc accctacacg
     2281 cccgaagcga aacagtggct caaaatccac ggcagggaac ttttcgcctt aggctgacgc
     2341 ggcccgggca ggcatccgcc aattcagacc tcctcccaaa cggacatccg gaaagcagca
     2401 tcacagccgg cgacacatac cgctgccgcc gctttcagac ggcatccgtg ccggaacagg
     2461 cacgcgcccg cccgattcaa accgcgatga cgctttgccg ccggttcggg gcaggatggc
     2521 ggcacacacg ccgtctgccg cgtttcattt cacaccgccc ttccgaaacc cgaaacccgc
     2581 ccggtccgac gtgcggtatg aaacgcttaa gctggcgcga agtcttttac tgatttgccc
     2641 gcgaaaatgc cgtctgaaag gttttcggac ggcatttttt tgcgtttccc aggatggcgg
     2701 cggattcgta aaaggcggtc agggtggatt gtaggatggg ttgagacctg ccgaatccgc
     2761 cgcatctgcc aaatctaccg ccgtcattcc tacgaaagtg ggaatctaga acgcgaggtt
     2821 aagaaaacct gcat
//
LOCUS       DAWKST010000197         2749 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.197, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000197 DAWKST010000000
VERSION     DAWKST010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.197"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            517..801
                     /locus_tag="WJL87_001902"
     CDS             517..801
                     /locus_tag="WJL87_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ7800301.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
     gene            1361..>2749
                     /locus_tag="WJL87_001903"
     CDS             1361..>2749
                     /locus_tag="WJL87_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ7800302.1"
                     /translation="MNGQKLPEVKNGNQGNQRQATFTTNFSRAKKNTTFTFNNTDDVV
                     AQKSSTVVFGTATYLPPYGKVSGFDENGLKERGDAVGWIGTTHPGLIGYSYQGNTCSS
                     GDCPELSYKTQFTFGHQGLKKKTGGKLDIDADKSRDNSPIYKLSDNSWLGVSFNLSSE
                     STAESKQNKKLVSSFSENVTQSNGTQGQNKDKNLVYNTDNQRHNNNNRVNQNSSHAVA
                     FYLNAKLHLLDKKQINNIAQGVTVDLGTLKTRIEPTDEWKNKRHLTFVVNQWEFKDAG
                     SVSVKLKLPEVKAGRCINKPNPNNHTKAPSPALTAPALWFGPVQNGKAEMYSASVSTY
                     PDSSSSRIFLQNLKRKTDPGRPGRYSLADLSASDIQSKEPTFTSRQTIIRLDKGVHQI
                     KLQGNEVANFNGNDGKNDTFGIVSEGSFMPDTSEWKKVLLPWTVRGFSNDNEFAKFNK
                     ESDKYSQRYRIRD"
BASE COUNT          826 a          608 c          682 g          633 t
ORIGIN      
        1 agaccttaga ataacagcaa tattcaaaga ttatctgaaa gtccgagatt ctagattccc
       61 actttcgtgg gaatgacggg atgtaggttc gcgggaatga cgggatttga gattgaggca
      121 tttatcggaa aaaacagaaa ccgctccgcc gtcattcccg cgcaggcggg aatctagacc
      181 ttagaacaac agtaatattc aaaggttagc tgaagcttta gagattctag attcccactt
      241 tcgtgggaat gacgggattt gagattgcag catttatcgg aaaaaacaga aaccgctccg
      301 ccgtcattcc cgcgcaggcg ggaatctagg tttgtcggtg cggaaactta tcgggtaaaa
      361 cggtttcttt agattttgcg ttctagattc ccactttcgc gggaatgacg aagagttgcg
      421 ggaatgatgg aaagctatgg gaataacgaa gggttaaagt aatcacggga tggtgttcgc
      481 gggaatataa atttaaataa ttcaaaaggg tgttatatgc agcctgcggt ttatatttta
      541 gcaagccaac gtaatggcac gttatacatt ggcgttacat ctgatttggt gcaacgtatt
      601 taccaacata gggaacattt gattgaggga tttacatcac ggtacaacgt tactatgctg
      661 gtttggtatg aactgcatcc tacgatggag agcgcaatta ctcgggaaaa acagttgaag
      721 aaatggaaca gggcttggaa attgcaactg attgaagaaa ataatgtttc ttggcaggat
      781 ttatggtttg atattattta gcccgtcatt cccgcgcagg cgggaatcta gaacgtaaaa
      841 tctaaagaaa ccgttttacc cgataagttt ctgtgccgac agacctagat tcccactttc
      901 gtgggaatga cggaatgtag gttcgtggga atgacgcggt gcaggtttcc gtgcggatgg
      961 attcgtcatt cccgcgcagg cgggaatcca gaccttggga taacagcaat attcaaaggt
     1021 tataaaagac ccgtcattcc cgcgcaggcg ggaatccaga ccgttgggca tctgcagcgg
     1081 tttgctaaaa aacgctttac cgtgatcagt gtgcaaagtt aaaatgggga ggtaagcttt
     1141 tcaaccagca attcggcggg cgcggaatcg ggcggtttac cgaaccccgg cgttcgcggc
     1201 gcgccgcccc gtcccgtgaa ggcaaactca aggaataaaa gatgaataaa acttggaaac
     1261 ggcaggtttt ccgccatacc gcgctttata ccgccatatt gatgttttcc cataccggcg
     1321 ggggggggcg caggcgcaaa tgtattcata cagtattgta atgaacggac aaaagctgcc
     1381 cgaagtaaag aatgggaacc aaggcaatca acgccaagct acctttacga caaattttag
     1441 tagagccaaa aaaaacacta ctttcacatt caataatacc gatgatgttg ttgctcaaaa
     1501 aagcagcact gtcgtttttg gtacagccac ctacctgccg ccctacggca aggtttctgg
     1561 ttttgatgaa aacgggctga aagaacgcgg cgatgccgtt ggttggattg gtacgaccca
     1621 cccggggttg ataggctaca gctaccaagg taacacatgc agcagcggag actgtcctga
     1681 acttagctat aaaacccaat ttaccttcgg tcatcaaggg ttgaaaaaaa agacaggcgg
     1741 caagctggat atagacgcag acaaaagccg cgacaattcg cccatttaca aattgtcgga
     1801 taattcttgg ttgggcgtgt ctttcaattt gagcagcgag agcaccgccg aatcaaagca
     1861 aaacaagaaa ttggtatctt cttttagcga gaacgtgacg cagagcaatg gtacgcaagg
     1921 acaaaacaaa gacaaaaacc tcgtgtataa cacggacaat caacgtcata ataataataa
     1981 tcgtgtcaat cagaacagca gccacgccgt cgccttttat ctgaacgcca agctgcacct
     2041 gctggataaa aaacaaatca acaatatcgc gcaaggcgta acagttgatt tgggtacctt
     2101 gaaaacgcgc atcgagccga cggacgagtg gaagaataag aggcatctca ctttcgttgt
     2161 taatcagtgg gagttcaaag atgccggatc agtcagcgtc aaactcaaat tgccggaagt
     2221 caaagcaggc cgctgcatca acaaaccgaa ccccaataat cataccaaag ccccttcgcc
     2281 ggcactgact gcccccgcgc tgtggttcgg acctgtgcaa aatggtaagg cggagatgta
     2341 ttccgcttcg gtttctacct accccgacag ttcgagcagc cgcatcttcc ttcaaaatct
     2401 gaaaagaaaa accgaccccg gcagacccgg ccgctattcc ctcgcagact tgagtgcgtc
     2461 ggatattcaa agtaaagagc cgactttcac aagtcggcaa acaatcatcc gattggataa
     2521 aggcgtacat cagatcaaac ttcaaggcaa tgaggtcgcc aattttaatg gaaatgacgg
     2581 caaaaacgac actttcggca ttgttagtga agggagcttc atgcctgata ccagcgagtg
     2641 gaaaaaagtg ctgctgcctt ggacggttcg gggttttagt aatgacaatg aatttgcaaa
     2701 attcaacaaa gaatcagaca aatacagcca aagataccgc atccgcgac
//
LOCUS       DAWKST010000198         2661 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.198, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000198 DAWKST010000000
VERSION     DAWKST010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.198"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            21..935
                     /locus_tag="WJL87_001904"
     CDS             21..935
                     /locus_tag="WJL87_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ7800303.1"
                     /translation="MKQINQALQQKLAEFKEKSGMSQTQLARGIGTSPASVSMYLNGT
                     YAEKGGNYETIEPKIEAFLEMQDSKAKREELVLGFVSTKTTRRIVEAMRNAHEGGEIS
                     VIYGQAGLGKTQAVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGHLIVVDEAENLPLRALEIIRRLHDETGCGLVLSGMPRLVANLRGKYG
                     ELVQLHSRVAGALNLGDTMPDAELEQIARATLPEADDETIAELVKHSNGNTRRMSKLM
                     RGSVRVANKNGIRMQAGIVKKYSTLIIR"
     gene            1025..1291
                     /locus_tag="WJL87_001905"
     CDS             1025..1291
                     /locus_tag="WJL87_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800304.1"
                     /translation="MQVLKKVDWKMFVARSFWQCVPVGLTAGVWCFVAGMALHSCAKE
                     PEPVAKEPTKVEAMERQADLEVKVWEGQYESMSVEEKMKGIVYE"
     gene            1400..1573
                     /locus_tag="WJL87_001906"
     CDS             1400..1573
                     /locus_tag="WJL87_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800305.1"
                     /translation="MSFGRRNTDWQAWGQHRRRATARMAQKSREREIEEYQAQFEDKD
                     GKGRLKTEGNKDE"
     gene            1566..1733
                     /locus_tag="WJL87_001907"
     CDS             1566..1733
                     /locus_tag="WJL87_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800306.1"
                     /translation="MNEKDLIEWLEDRGELMVMKKDGEGFVIAARAPDGMWKTAEAET
                     LAQAITLWEEV"
     gene            1797..2024
                     /locus_tag="WJL87_001908"
     CDS             1797..2024
                     /locus_tag="WJL87_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800307.1"
                     /translation="MKVYVFKISNENGKLKIELPEIPMGKQIDEVDLIAGLTTEFIAS
                     MLRDAQKDRRKFVIDASNQLAAIQTYQKIFN"
     gene            2039..2557
                     /locus_tag="WJL87_001909"
     CDS             2039..2557
                     /locus_tag="WJL87_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HEZ7800308.1"
                     /translation="MAKTRIKQPAIEAAQDKTEVTAFIRQIGDLQREVKRLETEAGDK
                     KAAIEEEYAAKAAPMCAEIMSLTERVAAYCEAHKDELTENGKTKTVDFTTGLIKWRIR
                     PPSVKVTGVAAVLAWLSEKSAFAEFVRTKKEIDKDTILNQKECFSDGQVPGIKIVSGV
                     EDFVIEPSEQIL"
BASE COUNT          795 a          516 c          772 g          578 t
ORIGIN      
        1 cttttaaaag gactaaaaaa atgaaacaaa tcaatcaagc attgcaacaa aaactggctg
       61 aatttaaaga aaaatcgggt atgagccaaa cccaactggc acgcggtatc ggtacttcgc
      121 cggcatccgt cagtatgtat ctgaacggta cttatgcgga aaaaggcggc aattatgaaa
      181 ccatcgagcc aaaaatcgag gcgtttttgg agatgcagga cagcaaagcg aaacgcgaag
      241 agctggtgtt gggttttgta tcgactaaga cgacacgccg aatcgtggaa gcgatgcgca
      301 atgcgcacga aggcggcgag atttccgtta tctacggtca ggcgggtttg ggcaagacgc
      361 aggcggtcaa aaactactgc gagaaaaacc ctgcagccat cttgattgag gctaatccga
      421 gctttacggc tttggtcttg atgcgcaagt tggcgacggc ggcgaaggta tcggcgatgg
      481 gcagcttgaa tgatttgttt gaatcggtat cggacagatt gcgtgattca ggccatctga
      541 ttgtggtcga tgaagcggaa aacctgccat tacgcgccct tgagattatc cgccgactgc
      601 acgatgagac tggctgcggt ttggtgttga gcggtatgcc ccgactggtg gcgaatttgc
      661 gcggtaagta tggcgagttg gtacagcttc acagtcgtgt ggcgggtgcg ctgaatctag
      721 gtgatacgat gcccgacgca gagttggaac agattgcgcg ggcaacccta cccgaagccg
      781 atgatgaaac gattgcggaa ctggttaagc atagcaatgg taatacacgg cgtatgagca
      841 agttgatgcg cggatcagta cgggtagcga ataaaaacgg tattcgaatg caagcgggaa
      901 tcgtgaaaaa atattcgaca ttgattatcc gataggccgt ctgaaacggt aagtctctga
      961 cagggctata tattttttac cctatgattt taataagtta ttgttttcta aaggaaaact
     1021 caaaatgcaa gttttgaaga aagttgattg gaagatgttt gtggcacgct ctttttggca
     1081 gtgtgtgccg gttggtttga cggcgggcgt gtggtgtttt gtggcgggaa tggcgttgca
     1141 ttcctgcgcg aaagaacccg aaccggttgc gaaagagccg acgaaggttg aggcgatgga
     1201 aagacaggcg gatttggagg tcaaggtatg ggaagggcaa tacgagtcaa tgagcgtcga
     1261 ggaaaaaatg aaagggattg tttatgagtg ataagccaat tttattgagc cctgcggcga
     1321 aaaaagaggc tttggcacgg gcggtaaagg aaatccgcgc gaaatatggc gataaggcga
     1381 ttgtgaaagg atgtgtgaaa tgagtttcgg acgacgtaat acggattggc aggcttgggg
     1441 acaacaccgc aggcgtgcga cggcgcgaat ggcgcaaaaa agccgggagc gcgaaatcga
     1501 ggaatatcag gcgcagtttg aagataagga cggcaaaggt cgtctgaaaa cggaaggaaa
     1561 caaagatgaa tgaaaaagat ttaatcgaat ggctcgaaga ccgtggcgag ctgatggtca
     1621 tgaagaagga cggcgaaggc tttgtgattg cagcgcgtgc gccggacggg atgtggaaga
     1681 ctgccgaggc ggaaactttg gctcaggcga taactttatg ggaggaagtg tgatgtttaa
     1741 gtttttgaga aaccgcgcga aagttgaagc aattttaaac aagaaaggaa agtgaaatga
     1801 aagtgtatgt ttttaaaatt agtaatgaaa acggtaagtt aaagatagaa ctccccgaaa
     1861 tcccaatggg taagcaaatt gacgaagttg atttgattgc tgggctaacc acggaattta
     1921 ttgcaagtat gttacgtgat gcccaaaaag atcgtcgcaa attcgtaatc gacgcatcaa
     1981 atcaattggc tgcaatccag acatatcaaa aaatcttcaa ttaaggaagg aagagaaaat
     2041 ggctaaaacc cgaatcaaac agcccgccat cgaagcggca caagacaaaa cggaagttac
     2101 tgcatttatc cgccaaatcg gcgacttgca gcgcgaagtc aaacgcctgg aaaccgaagc
     2161 cggagacaaa aaagcggcta tcgaagaaga atatgccgcc aaagccgcgc cgatgtgtgc
     2221 cgaaatcatg agcctgaccg aacgtgtggc cgcctactgc gaagcgcata aggacgagct
     2281 gacggaaaat ggtaaaacca aaaccgtgga ctttaccacc ggcctgatta aatggcgcat
     2341 ccgtccgcca tctgtcaagg taacgggcgt ggccgccgtc ctggcgtggc tctcggaaaa
     2401 atccgccttt gccgagtttg tccgcactaa aaaggaaatc gacaaagaca ccatcctgaa
     2461 tcaaaaagag tgtttttcag acggccaagt tccggggatt aagattgtga gcggcgtgga
     2521 ggattttgtg attgagccga gcgaacaaat tctataaaat caacagcctg atttcaattc
     2581 agtgtgttga aacgtgattg agcctacgga gcaggagctt gcctagccgg ttcaaatagg
     2641 ctttaaccca tgatttaaag g
//
LOCUS       DAWKST010000199         2655 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.199, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000199 DAWKST010000000
VERSION     DAWKST010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.199"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..1074
                     /locus_tag="WJL87_001910"
     CDS             <1..1074
                     /locus_tag="WJL87_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800309.1"
                     /translation="KVFTNIKGLKIPHTYIETDAKKLPKSTDEQLSAHDMYEWIKKPE
                     NIGSIVIVDEAQDVWPARSAGSKIPENVQWLNTHRHQGIDIFVLTQGSKLLDQNLRTL
                     VRKHYHIASNKMGMRTLLEWKICADDPVKMASSAFSSIYTLDKKVYDLYESAEVHTVN
                     KVKRSKWFYTLPVIILLIPVFVGLSYKMLSSYGKKQEEPAAQESAATEHQAVFQDKTE
                     GEPVNNGNLTADMFVPTLSEKPESKPIYNGVRQVRTFEYIAGCVEGGRTGCTCYSHQG
                     TALKEITKEMCKDYARNGLPFNPYKEESQGRDVQQSEQHHSDRPQVATLGGKPWQNLM
                     YDNWQERGKPFEGIGGGVVGSAN"
     gene            1289..2245
                     /locus_tag="WJL87_001911"
     CDS             1289..2245
                     /locus_tag="WJL87_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ7800310.1"
                     /translation="MNIIGLDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKAYIGMEATGIYYEKAADMLSSYYTVYVINPLKIKDYGKSRFNRTKTDKADSN
                     LIADYIKRHQDTLIPYQIPKNKALQKLINLKNQLQQHQKQIKNRLHSTEEDFIRNIHQ
                     DLIDTIQDKMEQVKIAISEQIKKQTDNNHYRNLQTIPSIGKDTASVLYAQLTEKHFKT
                     ANQFVSYAGLSPAIIQSGTSVRGRGRLSRYGNRRLKSTLYMPALCAYRFNAFPKLINN
                     LKKAGKPKMVIIVAIMRKLAKLAYYIVKTGQPYDAERHRLNQ"
BASE COUNT          938 a          552 c          571 g          594 t
ORIGIN      
        1 aaagtattta cgaacatcaa aggcttgaag ataccgcaca cctacataga aacggacgcg
       61 aaaaagctgc cgaaatcgac agatgagcag ctttcggcgc atgatatgta cgaatggata
      121 aagaagcccg aaaatatcgg gtctattgtc attgtagatg aagctcaaga cgtatggccg
      181 gcacgctcgg caggttcaaa aatccctgaa aatgtccaat ggctgaatac gcacagacat
      241 cagggcattg atatatttgt tttgactcaa ggctctaagc ttctagatca aaatcttaga
      301 acgcttgtac ggaaacatta ccacatcgct tcaaacaaga tgggtatgcg tacgctttta
      361 gaatggaaaa tatgcgcgga cgatcccgta aaaatggcat caagcgcatt ctccagtatc
      421 tatacactgg ataaaaaagt ttatgacttg tacgaatcag cggaagttca taccgtaaat
      481 aaggtcaagc ggtcaaaatg gttttatact ctgccagtaa taatattgct gattcccgtt
      541 tttgtcggcc tgtcctataa aatgttaagt agttatggaa aaaaacagga agaacccgca
      601 gcacaagaat cggcggcaac agaacatcag gcagtatttc aggataaaac agaaggcgag
      661 ccggtaaaca acggtaacct taccgcagat atgtttgttc cgacattgtc cgaaaaaccc
      721 gaaagcaagc cgatttataa cggtgtaagg caggtaagaa cctttgaata tatagcaggc
      781 tgtgtagaag gcggaagaac cggatgcaca tgctattcgc atcaagggac ggcattgaaa
      841 gaaattacaa aggaaatgtg caaggattac gcaagaaacg gattgccgtt taacccatat
      901 aaagaagaaa gccaagggcg ggatgtccag caaagtgagc agcaccattc ggacagaccg
      961 caagttgcca cgttgggcgg aaagccgtgg caaaatctta tgtatgataa ttggcaggag
     1021 cgcggaaaac cgtttgaagg aatcggcggg ggcgtggtcg gatcggcaaa ctgaagaaaa
     1081 cggcaagaga gaaaaaagac ccgtaaaccg tttgaatata gacggcttac gggtctttgt
     1141 ttcgcgcaaa gcaagggcta aggcagtcag gcagcaaatc ccgcaatgta ttaaaacaga
     1201 cgcgtagaaa tgccggctgc ctttatccat cctcaaaatt gaatatcatc ctagccgtat
     1261 caaggctgta taaataagga aaataccaat gaatataatc gggctggaca tctcaaagga
     1321 taccatagac gcaacattgc ataaaacaaa cggaagtatc cattacatta aatttaagaa
     1381 taatgatgat ggattaaaac agtttagatt gtggataaag ggaaacagaa tcagaaaagc
     1441 ctatatcggc atggaggcaa caggcatcta ttacgaaaag gcagcagata tgctttcttc
     1501 ctactatacc gtttacgtta tcaatccctt aaaaatcaag gactacggga aaagcaggtt
     1561 taaccgtacc aaaaccgaca aagcagattc aaacctgata gcagattaca taaaaaggca
     1621 tcaagataca ttgataccgt atcagatacc caaaaacaaa gcactgcaaa aactgattaa
     1681 ccttaaaaat caattacagc aacatcagaa gcaaattaaa aaccgtcttc atagcactga
     1741 agaagacttc ataaggaaca tacatcaaga cttgatagat accatacagg acaagatgga
     1801 acaggtaaaa atagccatat ccgaacaaat caaaaaacaa acggataata accattaccg
     1861 caatcttcaa accatcccga gcataggcaa agacaccgca tcagttcttt atgcgcaact
     1921 tacagaaaaa cattttaaaa ccgcaaacca gtttgtatcc tatgccggat taagtcccgc
     1981 catcatacaa tcagggacaa gcgtaagagg tcggggcaga ttgagccgat acggaaacag
     2041 acgattaaaa agtacgctgt atatgcccgc cctttgtgct taccgtttta acgcatttcc
     2101 gaaattaata aataatctga aaaaagcggg taagccaaag atggtaatca tcgttgccat
     2161 catgcgcaaa ctggcgaagc tcgcctatta cattgttaaa accggccagc cttacgatgc
     2221 ggaaagacac cgattgaatc aataaaattc aacaaaatta aacggttacg cgaatatatt
     2281 tgtgtaaccg tgcatttgca tatcgtaaat aaacgtaaat aaaaataaca atataaatca
     2341 gtatattgca actttgtttt ttattttgtg ttgacgggca acatatcatc tgcgcgggaa
     2401 tgacgaatcc atccgcacgg aaacctgcac cacgtcattc ccactaacct acattccgtc
     2461 attcccacga aagtgggaat ccaggacgaa aaatctccag aaaccgtttt acccgataag
     2521 tttccgcacc gacaggtctg gattcccgcc tgcgcgggaa tgacgatgca ggtatttctg
     2581 acgattcccg gctatgatgt tgaggcagaa atcgaaaaat tcgtttggat ggatgctgtg
     2641 atttggcaga tgccg
//
LOCUS       DAWKST010000200         2637 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.200, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000200 DAWKST010000000
VERSION     DAWKST010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.200"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..105
                     /locus_tag="WJL87_001912"
     CDS             <1..105
                     /locus_tag="WJL87_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ7800311.1"
                     /translation="KVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            118..981
                     /locus_tag="WJL87_001913"
     CDS             118..981
                     /locus_tag="WJL87_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HEZ7800312.1"
                     /translation="MENVETHSQNHRKRLIGTFFLVGLENLLMLVYPVFGGWAINAVI
                     AGQAWQALLYALVVLLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAVT
                     ARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLPR
                     FAAISENLYFRLKNSLERDNHFIRKGDERQLDRHYGLLARLRVLISNREAFGYLCVGT
                     AMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQRI
                     EWSKRNIKAGT"
     gene            complement(1032..2084)
                     /gene="sohB"
                     /locus_tag="WJL87_001914"
     CDS             complement(1032..2084)
                     /gene="sohB"
                     /locus_tag="WJL87_001914"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ7800313.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSSETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(2188..2277)
                     /locus_tag="WJL87_001915"
     tRNA            complement(2188..2277)
                     /locus_tag="WJL87_001915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2241..2243),aa:Leu,seq:taa)
     gene            complement(2380..2453)
                     /locus_tag="WJL87_001916"
     tRNA            complement(2380..2453)
                     /locus_tag="WJL87_001916"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2419..2421),aa:Cys,seq:gca)
     gene            complement(2479..2554)
                     /locus_tag="WJL87_001917"
     tRNA            complement(2479..2554)
                     /locus_tag="WJL87_001917"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2519..2521),aa:Gly,seq:gcc)
BASE COUNT          622 a          708 c          627 g          680 t
ORIGIN      
        1 aaagtatttg ccacattcgg cgacaagcgc acaacccggc tgtttgccga tttggatgca
       61 ctggctgaag tgatggaaaa aacaatctcg gaaaataaaa aatagggggg caaatatgtg
      121 gaaaatgttg aaacacatag ccaaaaccac cgcaagcgat tgattggcac attttttctg
      181 gtcggactgg aaaacctttt gatgctggtg tatccggtgt ttggcggctg ggcgattaat
      241 gccgtgattg cggggcaggc gtggcaggcg ttgctgtacg ctttggttgt gcttttgatg
      301 tggctggtcg gtgcggcgcg gcggattgcc gatacgcgca cgtttacgcg gatttatacc
      361 gaaatcgccg tgccggttgt gttggaacag cggcagcggc aagtcccgca ttcggcggta
      421 actgcgcggg ttgccctgtc gcgtgagttt gtcagctttt ttgaagaaca cctgccgatt
      481 gccgcgacat ccgtcgtatc catattcggc gcgtgcatca tgctgctggt gctggaattt
      541 tgggtcggcg tgtcggcggt gggcatactt gcgttgtttt tatggctttt gccacgtttt
      601 gccgccatca gcgaaaacct gtatttccgc ctgaagaaca gcttggaacg cgacaaccac
      661 tttatccgaa aaggcgacga gcggcagctg gaccgccatt acggactgct tgcgcgcctg
      721 cgtgtgctga tttccaaccg cgaagccttc ggctatctct gcgtcggcac ggcgatgggt
      781 attttgttcg gctttgcttt tgtgatgatg acgctcaaag gctacagcag cgcggggcat
      841 gtctattcgg tcggcactta tctgtggatg tttgccatga gtttggacga cgtgccgcga
      901 ttggtcgaac aatattccaa tttgaaagac atcggacaac ggatagagtg gtcgaaacgg
      961 aacatcaaag ccggaacttg aaaaatgccg tctgaacacg cttcagacgg catttccatc
     1021 cgttcggcaa actacatcac atccgcccgc cggttgacaa gtttggcaaa caacttttca
     1081 acagaagctt ccgcctgcaa accaatgcgc tggatcaggc tttgcttctc ctgatatttc
     1141 acttcgataa cctgtttgtt ttcaaacgct ttcaacaaca aatcatcact ggtcgaaatc
     1201 tcgtcaatca agttcaacgc caacgcctgc cgaccgaacc aatgctcgcc cgttgccact
     1261 tcctcaatat ccaattgagg gcggttctcg ctgacaaact gcttgaacaa ctgatgcgtt
     1321 tcctccagtt cctgtcggaa tttctgtttg cccttttccg tattttcacc cataaaagta
     1381 accgtgcgct taaattcgcc cgccgtcatc acatccacat caatatcatg ttttttcaac
     1441 aggcggtgga tattcggtac ttccgctaca acacccaccg aaccgacaat cgcaaacgga
     1501 gcggaaacaa ttttatccgc cacacacgcc atcatataac caccgctcgc cgccacctta
     1561 tcgacggcga cggtcagcgg aatattgcgt tcgcgcaaac gcctaagctg cgaagccgcc
     1621 aaaccgtaac cgtgaaccac gccgcccgga ctttccaatc taagcagaac ctcatcttca
     1681 ggcttggcaa tcaaaagcac cgccgtaatc tcatgacgca aggattctac ggcgtgtgca
     1741 tacaaatcgc cgtcaaaatc caacacaaaa aggcgggatt tttgcgtttc ggaagatttc
     1801 tccccaccct ccttcaaacg ctttttctct gctttggctt ccgccttttc ctttttcttt
     1861 tcctcttttt cctgatgttt tgcctcttcc ccgcttaaaa agaatgcttc aaacgattgc
     1921 cgctgttttt tataattttc cgaaaaatcc gtcagtacga cactgccgct ttccgactgt
     1981 ttcttactct gtacgatagc caacacaatc agcgcaattg cgccgaacac ggtaagcagt
     2041 tcgagcagga aaataccgta attcagtaaa atttctttcc acattgattg gatttcctct
     2101 tgttcaggca tgaacatgtc aatattgtcc atcaccgtcc gacagataaa aaaataaccg
     2161 cttggagcgg cattgtcatt ttcagcttgg tgcccggagc cggaatcgaa ccggcacggg
     2221 atgtttagtc ccgacggatt ttaagtccgt tgtgtctacc tatttcacca cccgggcatt
     2281 tgtgaaaggt ggaggcgggg gcgcggattt taaccggcct atatgaagat tgcactcctc
     2341 atagcataaa cactctgcca ccccgccgta gtacgataat ggaggcgaga gtcggaatcg
     2401 aaccggcgta gacggatttg caatccgctg cataaccact ttgctatctc gccctaaaac
     2461 tggcttatct aaaaaacttg gagcgggaaa cgagtctcga actcgcgacc tcaaccttgg
     2521 caaggttgcg ctctaccaac tgagctattc ccgcgcgttc aaacatatcg gtttttggag
     2581 cggaaaacga gtctcgaact cgcgacctca accttggcaa ggttgcgctc taccaac
//
LOCUS       DAWKST010000201         2578 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.201, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000201 DAWKST010000000
VERSION     DAWKST010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.201"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            74..703
                     /locus_tag="WJL87_001918"
     CDS             74..703
                     /locus_tag="WJL87_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ7800314.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGVYILIENNSFDMLWTVSGQEKIL"
     gene            849..2510
                     /locus_tag="WJL87_001919"
     CDS             849..2510
                     /locus_tag="WJL87_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ7800315.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPVAHFLNPTAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDDHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIDIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNADASQGWQTVWQGARPRNKDSKFALIRKIGENI"
BASE COUNT          506 a          659 c          670 g          743 t
ORIGIN      
        1 cggggttgaa gttgacatct acaaagttta atgtttgaat acattaaaaa aatatatttg
       61 aatgagatgt tttatgattc aaaatgttgt tacttcaata atcctgtatt ctgggacagc
      121 cgtagactta cttattatcc taatgttatt ttttgccaaa agaaaaagca gaaaagacat
      181 cattaacatc tatttaggac aatttctagg ctctgttagc ctgatattgc taagtttgct
      241 ttttgcattt gtcttagatt atattcctag taaagagatt ttaggtttgc tcggcttgat
      301 tccaattttc ctaggcatca aagttttgct gttaggagat tctgatggag aggctattgc
      361 caaagagggt ttgcgcaaag ataataaaaa cctgattttt ctagtcgcta tgattacttt
      421 tgcaagttgt ggtgctgaca atattggtgt ctttgtccca tattttacta ccttaaattt
      481 agcgaatttg atagtggctt tacttacctt tctagtcatg atttatctct tggttttttc
      541 tgcccaaaaa ttagcacaag tcccttctgt tggagaaact ttggaaaaat atagcagatg
      601 gtttgttgct gttgtttatt taggattggg ggtatatatc ctgattgaaa acaacagttt
      661 tgatatgcta tggactgtgt cgggccagga aaaaatatta tgaaaaagat agtatctgca
      721 aagactgcca tgtgcagtct tttttgttgc cggtcttttt gtgtctgatg ccgtctgaag
      781 cagtctctgc acgacctttg tgcgaatatt tgctacactt ggcaatccgt ccatttcttc
      841 tcacgcttat gctgacctac actccgcccg atgcccgccc gcccgccaaa acccacgaaa
      901 agccgtggct gctgctgttg atggcgtttg cctggttgtg gcccggcgtg ttttcccacg
      961 atttgtggaa tcctgccgaa cctgccgtct ataccgccgt cgaagcactg gcaggcagcc
     1021 ccaccccttt ggttgcccat ctgttcggtc aaaccgattt cggcataccg cccgtgtatc
     1081 tttgggttgc cgccgcgttc aaacatttgc tgtcgccgtg ggcagccgac ccgtatgatg
     1141 ccgcacgctt tgccggcgtg tttttcgccg ttgtcggact gacttcctgc ggctttgccg
     1201 gtttcaactt tttgggcaga caccacgggc gcagcgtcgt cctgattctc atcggctgta
     1261 tcgggctgat tccagttgcc catttcctca accccactgc cgccgccttt gccgccgccg
     1321 gactggtgct gcacggttat tctttggctc gccggcgcgt gattgccgcc tcttttctgc
     1381 tcggtacggg ctggacgctg atgtcgttgg cagcagctta tccggcagca tttgccctga
     1441 tgctgccctt gcccgtactg atgtttttcc gtccgtggca aagcaggcgt ttgatgttga
     1501 cggcagtcgc ctcacttgcc tttgccctgc cgcttatgac cgtttacccg ctgctcttgg
     1561 caaaaacgca gcccgcgctg ttcgcgcaat ggctcgacga tcacgttttc ggtacgttcg
     1621 gcggcgtgcg gcacgttcag acggcattca gtttgtttta ctatctgaaa aacctgcttt
     1681 ggtttgcact gcccgcgctg ccgctggcgg tttggacggt ttgccgcacg cgcctgtttt
     1741 cgaccgactg ggggattttg ggcgtcgtct ggatgcttgc cgttttggtg ctgcttgccg
     1801 tcaatccgca gcgttttcag gataacctcg tctggctgct tccgccgctt gccctgttcg
     1861 gcgcggcgca actggacagc ctgaggcgcg gcgcggcggc gtttgtcaac tggttcggca
     1921 ttatggcgtt cggactgttt gccgtgttcc tgtggacggg ctttttcgcc atgaattacg
     1981 gctggcccgc caagcttgcc gaacgcgccg cctacttcag cccgtattac gttcccgaca
     2041 tcgatcccat tccgatggcg gttgccgtac tgttcacacc cttgtggctg tgggcgatta
     2101 cccgcaaaaa catacgcggc aggcaggcgg ttaccaactg ggcggcaggc gttaccctga
     2161 cctgggcttt gctgatgacg ctgttcctgc cgtggctgga cgcggcgaaa agccacgcgc
     2221 cggtcgtccg gagtatggag gcatcgcttt ccccggaatt aaaacgggag ctttcagacg
     2281 gcatcgagtg tatcgacata ggcggcggcg acctgcacac gcggattgtt tggacgcagt
     2341 acggcacatt gccgcaccgc gtcggcgatg tacaatgccg ctaccgtatc gtccgcttgc
     2401 cccaaaatgc ggatgcgtcg caaggctggc agacggtttg gcagggtgcg cgcccgcgca
     2461 acaaagacag taagttcgca ctgatacgga aaatcgggga aaatatataa aaaacaacag
     2521 attgagccga atttctggat taagtgccgg aaatcgcgta taattgcgcg attaaacc
//
LOCUS       DAWKST010000202         2558 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.202, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000202 DAWKST010000000
VERSION     DAWKST010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.202"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            68..847
                     /locus_tag="WJL87_001920"
     CDS             68..847
                     /locus_tag="WJL87_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ7800316.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALTEHPLVGEYGHSFRFLPVTTRAANPSGLSGKRIPELLKNNSIEQALHTKFTPES
                     TRFMICGNPDMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(1057..2469)
                     /locus_tag="WJL87_001921"
     CDS             complement(1057..2469)
                     /locus_tag="WJL87_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ7800317.1"
                     /translation="MLFEFKENKMIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVVGLTGISDGMVADAPVFAEIAGELF
                     SVLKGCVLVAHNSRFDYTFLKHEFHRVGIGFSSPVLCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDGMGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFP
                     ALGSLHAYKIKAELVGRYHSGCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVT
                     DNPPTGLFANKKAARRALSSWAETYGLCPAAAGILSDGYTEDEPCPVYVSDRCDKACV
                     RSDEQVLAFAHKLPVLDWGKMHEVEITETDPLTGEKVVLHGMGGALEMDDGLWYFDKD
                     LPDAFKAKFKTDRKNIKEIG"
BASE COUNT          712 a          784 c          475 g          587 t
ORIGIN      
        1 accgtcaatg cggaaaaaaa cacccaaatt ggctataatc ccgacaaaca cactcaagga
       61 caacaacatg gcagcctcgc ccgaagcaaa attcactgaa gaaaagattt tgtgggtcaa
      121 acaccacacg ccgaaactca tcactttcgc catcagccgt cccgaatcct accgttttaa
      181 agcgggacag ttctcccgac tcggtttcta cgaaggggaa ggctttattt ggcgtgccta
      241 ctccatcgtt tccgcagaat atgccgacac gctcgaatat tttgccgtac tcatccaaga
      301 cggccccatg tctgcccgtt tcgccaaaat gcaacagggc gacaccatcc tgctcgataa
      361 aaatgccacc ggcttcctgc ttcccgaacg cttccccgac ggcaaggatt tggtgatgct
      421 ctgcaccggc tccggcatcg cccccttcct ttccattctc gaacaacctg aaatccggca
      481 gcgtttcgat accgtcaacc tgatacattc cgtatctttt cccgaagaat tgattttcaa
      541 cgaccggctc gccgcattga ccgaacatcc cctggtaggc gaatacggac actcgttccg
      601 tttcctccct gttaccaccc gtgccgccaa cccctcgggc ttaagcggaa aacgcattcc
      661 ggaactctta aaaaacaaca gcatcgaaca ggcgctgcat accaagttca ccccggaatc
      721 cacacggttt atgatttgcg gtaacccgga catggtcaaa gacactttcc aaacgctgct
      781 tgacatgggt tacgccatgc accgcaaccg cattcccggt caaatcatga tggaaaacgg
      841 cttctaaaaa ccaccctgct tgtccgatgc cttcggatgg acggtcaaac cgacacggca
      901 cgaaaaccgc gtcggcaaaa atgccgtctg aaaagttcag acggcatctt cggacacatt
      961 acctgcaaac ggtaacacat tccaacccaa accgattagg caatcagtac gaacgactgt
     1021 ttacatactt gccggctttc accaaccggt atcgatttaa ccgatttcct taatattttt
     1081 cctgtccgtt ttaaacttcg ccttaaacgc atccggtaaa tctttatcga aataccaaag
     1141 cccgtcatcc atttctaatg cgccgcccat tccatgcaga acaacttttt cccccgttaa
     1201 aggatcggtt tcggtaattt ccacctcatg catttttccc caatccaaga caggcagctt
     1261 gtgtgcaaac gccaaaacct gttcgtcaga gcggacgcac gccttatcac atcggtctga
     1321 aacatatacg ggacaaggct catcctctgt ataaccgtcc gacaggatac cggctgcggc
     1381 aggacacaat ccgtatgttt ccgcccacga cgacaaagcc cgtctcgctg cctttttatt
     1441 ggcaaataat ccggtaggcg gattatccgt cacaccgttt ttcaaagccg ctgttttcgc
     1501 attcaacatg ccgtctgaac ctttttcaaa cctgacggtc gtaaatgttt taagcagatt
     1561 tttggcagat acataacaac ccgaatgata acgtccgacc aattccgctt taatcttata
     1621 tgcatgcagg ctgcccaatg caggaaaaaa acggacttcc tccgtattgc accaatcaaa
     1681 cggggctttt ccggaatcca ataaagccga aacctcgcta tatacccgtt caaacgtacc
     1741 gatgtaattt acttttcctc cgccgtcgaa acaggccagc acccccatac cgtcaggcaa
     1801 accgtacaac tgttccctca accgttcggg cagcgcggca ggcaacggtt tcggattcat
     1861 caaacggaaa cactgcctga tccatgcctc gaccccgtgt tccgacagac tgtattccaa
     1921 ataatcgcac aatgccgata catccgccat cgcacgatgc ctgtcttcca caacaatccc
     1981 caacctttcg atgatactgt ccaggctgtg cttgtaaaat tgcggataca gacaccggga
     2041 cagctgcaca ctgcacaaaa caggcgatga aaatccgata cccacacgat gaaactcatg
     2101 ctttaaaaac gtatagtcga aacggctgtt atgtgcaacc agcacacaac ccttcaatac
     2161 cgaaaacaac tcgccggcaa tctctgcaaa aacaggcgca tcggcaacca tgccgtctga
     2221 aatccccgtc agccccacca caaactgcgg aatcggtttt tgaggattaa ccaaccactc
     2281 atgcctcacc acccttccct gctcaaactt gaccaaagcc acttcggtta ccctgtcttc
     2341 atacagattg ccgcccgtcg attccaaatc aaccacggca acaggcattc caaaccgtaa
     2401 aaataccttt tccagcaagg gccagcgaga agcaacaatc attttattct ctttaaattc
     2461 aaacaacaaa ccaatatttt acacttttaa ggcatttcat ccaacaaaac aattgacaga
     2521 atccgatgat taccctaaaa ttcgaatctt tcttgcag
//
LOCUS       DAWKST010000203         2497 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.203, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000203 DAWKST010000000
VERSION     DAWKST010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.203"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            168..791
                     /gene="lolA"
                     /locus_tag="WJL87_001922"
     CDS             168..791
                     /gene="lolA"
                     /locus_tag="WJL87_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ7800318.1"
                     /translation="MMKPHNLFQFLAVCSLTVSVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            1064..2203
                     /locus_tag="WJL87_001923"
     CDS             1064..2203
                     /locus_tag="WJL87_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7800319.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQAPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYSNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            2336..>2497
                     /locus_tag="WJL87_001924"
     CDS             2336..>2497
                     /locus_tag="WJL87_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7800320.1"
                     /translation="MDLSIVVPIYNVESYLEACLSSIEPILSNENVELILVNDGSKDG
                     SEDICYKYID"
BASE COUNT          675 a          734 c          620 g          468 t
ORIGIN      
        1 gcggcacaca attaaagggc agcgtggcgc gccgcctttt ccggtgggca aaaaatcagc
       61 cctcggaaaa cgcggtttgc aaaatgcaaa ccgaccgtaa cgccacccgt atgattgttt
      121 tgctgcgccg atactttacg ccacacccat cccgacaagg aaaaataatg atgaaaccgc
      181 acaacctgtt ccaattcctc gccgtttgct ccctgaccgt ctccgtcgct tccgcacagg
      241 cgggcgcggt ggacgcgctc aagcaattca acaacgatgc cgacggtatc agcggcagct
      301 tcacccaaac cgtccaaagc aaaaagaaaa cccaaaccgc gcacggcacg ttcaaaatcc
      361 tgcgcccggg cctcttcaaa tgggaataca cttcgcccta caaacagact attgtcggcg
      421 acggtcaaac cgtttggctc tacgatgtcg atttggcaca agtgaccaag tcgtcccaag
      481 accaggccat aggcggcagc cccgccgcca tcctgtcgaa caaaaccgcc ctcgaaagca
      541 gctacacgct gaaagaggac ggttcgtcca acggcatcga ttatgtgctg gcaacgccca
      601 aacgcaacaa cgccggctac caatacatcc gcatcggctt caaaggcggc aacctcgccg
      661 ccatgcagct taaagacagc ttcggcaacc aaacctccat cagtttcggc ggtttgaata
      721 ccaatcccca actctcgcgc ggcgcgttca agtttacccc gcccaaaggc gtggacgtgt
      781 tgagcaactg atgccgtccg ccccgatgcc gtctgaaagc cgccgaggct tcagacggca
      841 ttttcacgca ggcggaacaa tgtcccacat taccgcccga tcgggcatcg gaacggcaaa
      901 cccgtgaaaa ttaacggttg cgccccggct gtttttgccg tttaatgcaa accttgctgc
      961 accaagggcc aagaaagccg accggccgcc cccacagctt ccgatgcagg cggcccggcc
     1021 gtccctgcaa tgttttttat ttttgaacga aaggtcgaaa accatgaaaa aaacactggt
     1081 ggcggcggca atcctgagcc tcgccttgac tgcgtgcggc ggcggaagcg ataccgccgc
     1141 ccaagccccc tccgccaagc ctgaagccga acaatcgggc aaactcaata tctacaactg
     1201 gtcggattat gtcgatcccg aaaccgtcgc cgcctttgaa aaagaaaccg gcatcaagac
     1261 gcgttccgat tattacgaca gcaacgaaac actggaggca aaagtcctga ccggcaaatc
     1321 cggctacgac ctgaccgcgc cgtccatcgc caacgtcggc cggcaaatca aagcgggcgc
     1381 gtatcagaaa atcgacaagg cgcaaatccc ccattacagc aacatcgata aagatttgct
     1441 gaaaatgatg gaagccgtcg atccgggcaa cgaatacgcc gtcccctatt tctggggcat
     1501 caacaccttg gcaatcaaca cgcggcaggt gcaaaaggca ttgggtacgg acaagctgcc
     1561 cgaaaacgaa tgggatttgg tgttcaaacc cgaatacacc gccaaactca aatcctgcgg
     1621 catcagctat ttcgacagcg caatcgaaca gattcccttg gcgttgcact atttgggcaa
     1681 agatcccaac agtgagaatc ccgaagacat caaagccgcc gtcgatatga tgaaagccgt
     1741 ccggggcgac gtgaaacgct tcagctcttc cggctacatc gacgatatgg cggcgggcaa
     1801 cctgtgtgcc gccatcggtt acggcggcga tttgaacatt gccaaaaccc gtgccgaaga
     1861 agccgcaaac ggcgtggaaa tcaaagtatt gaccccgaaa accggcgtgg gcgtgtgggt
     1921 ggattccttt atgattccgc gcgacgcgca aaacgtcgcc aacgcccacc gctatatcga
     1981 ctacacgctc cggcccgagg tggcggcgaa aaacggcagc ttcgttacct acgcgcccgc
     2041 cagccgtccc gcgcgcgagc tgatggatga aaaatacacc tccgacgcat cgattttccc
     2101 gaacaaagaa ctgatggaaa aaagtttcat cgtatcgccc aaatccgcag aatccgccaa
     2161 actgggcgtg aagctgtggc aagggctcaa agcgggcaaa taaccgcaac ccctgccgtc
     2221 tgaaaccttt cgggcggcag gaaacggcgc gtccgttatc aaacaggggg gcgtttcccc
     2281 tcctgccggt tatgattggg ttaagattaa aatgatttag taaaatgaga aagatatgga
     2341 tttaagtatc gtagttccca tttataatgt cgaaagttat ttggaagcgt gtttaagttc
     2401 catagaacct atattaagta atgaaaatgt cgaacttatc cttgtgaatg acgggtcaaa
     2461 agacggaagt gaagatatat gttataaata tatagat
//
LOCUS       DAWKST010000204         2476 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.204, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000204 DAWKST010000000
VERSION     DAWKST010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.204"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(13..819)
                     /locus_tag="WJL87_001925"
     CDS             complement(13..819)
                     /locus_tag="WJL87_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ7800321.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRSF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1039..2409
                     /gene="ffh"
                     /locus_tag="WJL87_001926"
     CDS             1039..2409
                     /gene="ffh"
                     /locus_tag="WJL87_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ7800322.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVAGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTAGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMKGMK
                     GMFPGL"
BASE COUNT          681 a          626 c          644 g          525 t
ORIGIN      
        1 tgttggctct tcttatcttt tcagaatgat ttccaatacg aacttgctgc ccatataggc
       61 aatcataagg ctgacaaatc cgatgatggt ccacacggcg gcttttttac cgcgccatgc
      121 ggtcatgctg tgcttgagca gcagtccgcc gtaaatcagc catgacaata tgccgaatac
      181 ggttttatgg gtaaaggtca tgggtttgcc gaatacggct tcggcgaaga atgtgccgct
      241 gacgacggaa taggtcagca ggatgaaacc tgcccacatg gcctggaaca tgagtttttc
      301 caaactgagc agcgacggca ggaatcctgc gagcttggag aagctcctgc ggtgcaggct
      361 ccgattcagc agcagggtca aaacggacaa taatgttgcg atgccgaaca gcccgtatgc
      421 aagcagcgaa gttccgatat gcagcataaa gggaaggtcg gtaatttcat atcccgagaa
      481 ttttccagga aaaaccaaac ctgacagcag catcagtgcg gcgcaaggat acagcagcaa
      541 ctgcactccg cgcagcggat aaaagaagct gccggcaaaa taaataaaca gcatcatcca
      601 aacaatcagg ctgccggaat acccgaagcc cataatgatg attttgtctt gaatgaccgg
      661 cataagcagt gccgcgccgt ggacggtcaa tgccgcgccc aaaaccggca attccgtctt
      721 ccacgggtaa tcccggccgc gcccctgctg ttggcagtgc catgcaaatg cacccaatcc
      781 tgcgtaaacc gccgtcaaaa agatgaaaac tgtcggcatg gtggactttc tctatctata
      841 ctgttgcgcc gtctgcggcc gcttatgaaa tattggaact tttaacgttg gaattgtaaa
      901 atccccgttt cgggcaagcc ttgacggatt tgccgatatg ctgtccggca cacaagccgc
      961 atcaaattat tttgatttta ttttaacaaa gaatgcccct gatggggcaa gctattctta
     1021 ttcagaccaa ggaccagtat gttagacaat ttaaccggcc gcttcagcaa tgtcttcaaa
     1081 aacatccggg ggcaggccaa actgaccgaa gacaatatta aagaggcctt gcgcgaagtc
     1141 cgcctcgccc tgcttgaggc ggatgtcgcc ctgcctgtcg tcaaagagtt cgtcaacaac
     1201 gtcaaagaaa aggccctcgg tcaggaagtg gctggcagcc tgacgccgga tcaggcattt
     1261 atcggcgtgg tcaacaaagc cctgaccgaa ctgatgggca gggaaaacaa aacgctggat
     1321 ttgtcggttg cgccgcccgc cgtcgtgttg atggcaggtt tgcagggcgc gggcaagacg
     1381 acgaccgccg gcaaactcgc ccgcctgctg aaaaacgatc agaagaaaaa ggttttggtc
     1441 gtatctgccg acgtttaccg ccctgccgcg attgaacaac tgcgcctgtt ggccgaacag
     1501 gtcggcgtgg actttttccc atccgatacc aaccaaaaac cggttgaaat tgcaactgcc
     1561 gccgtcgatt acgccaaaaa acatttttac gatgttttga tggtcgatac cgccggccgt
     1621 ctggcaatcg atgaagagat gatgaacgaa atcaaagccc ttcacgcggc ggttaacccg
     1681 gtggaaactt tgttcgtcat cgatgcgatg ctgggtcagg atgcggtgaa cactgctcag
     1741 gcatttaatg aagccctgcc gctgaccgga gtcgtattga ccaagatgga cggcgactcg
     1801 cgcggcggtg cggcattgtc cgtacgccat gtaaccggca aaccgattaa atttatcggt
     1861 gtcggcgaaa aaatcaacgg cctcgaacct ttccaccccg accgtcttgc cagccgtatc
     1921 ctcggcatgg gcgacgtatt gaccctgatt gaagacgttc aaaaaggtat tgatgaagaa
     1981 gccgccgcca aaatggcgaa aaagctgcaa aaaggcaaag gcttcgacct caacgacttt
     2041 aaagaacaaa tccagcaaat gcgcaatatg ggcggtttgg aaaacctgat gtcgaaaatg
     2101 ccgggcgaac tgggtcaaat atcgaaacaa atccccgaag gtacggctga aaaagcgatg
     2161 ggtaaagtag aagccatcat caactcgatg acccctaaag aacgcgccaa ccctgccctg
     2221 ctcaaagcca gccgcaaacg ccgtattgca atgggtgcgg gcacgaccgt acaggaagtg
     2281 aacaaattgc tcaaacagtt tgaacaaatg caacaaatga tgaagatgtt cagcggcaac
     2341 ggcttgggca aactgatgcg tatggcgaaa ggaatgaagg gaatgaaagg aatgttcccg
     2401 ggtttgtaag ccgatttgac cgaaaacgcc gtctaaaatt tcagacggcg tttttgtttt
     2461 atattctgat ttatag
//
LOCUS       DAWKST010000205         2441 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.205, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000205 DAWKST010000000
VERSION     DAWKST010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.205"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..380)
                     /locus_tag="WJL87_001927"
     CDS             complement(<1..380)
                     /locus_tag="WJL87_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800323.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGKWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMY"
     gene            complement(438..803)
                     /locus_tag="WJL87_001928"
     CDS             complement(438..803)
                     /locus_tag="WJL87_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800324.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(785..1330)
                     /locus_tag="WJL87_001929"
     CDS             complement(785..1330)
                     /locus_tag="WJL87_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800325.1"
                     /translation="MKKQITAAVMMLSMIAPAMANSMDNQIFENQVFHTRADAPMQLA
                     ELSQKEMKETERAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIPG
                     GVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQ
                     GIGRHRPWESKSTDRSWKNRF"
     gene            complement(1493..1765)
                     /locus_tag="WJL87_001930"
     CDS             complement(1493..1765)
                     /locus_tag="WJL87_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800326.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYIIGIINVIIG
                     YVLIKNILVSVSDGGGIKYVAIYLSNLFFWTVLMYVLVKRLSKKPS"
BASE COUNT          772 a          549 c          359 g          761 t
ORIGIN      
        1 ctatacatct caatacgata aatatttctt aagtcaaaat gcaagcctga ccgtacctta
       61 actgtcaaaa ttttattatt ttttattgat tttaaaacaa tttctgcaaa attctcttcg
      121 ctttctctct tttttagaag cacataagaa aaaataaaac ttccccgatt aaattcataa
      181 atatgtttca accatttgcc tcctctttct gtaagacaag attcagtttc attcttcctt
      241 attgtataaa tatttccttc acaaaatctg aaataaatcc ataaatccat cttatccata
      301 attaaagaaa aagtttcacc tcgagatttt gtcaacaatt cgcaaggttg cgatgttgca
      361 atcaaatagc cgaaagacat tttttacctc atacatggtc gaaatcagtt tctgttagtt
      421 cagaatccat tttttcgtca acaactgaat ccgcattttt gaattaacgt tttcatcagc
      481 tgccgtttat ctaaaccggc aggttcagtt tcaaaataag ccttatatga agactgtaag
      541 catttcagaa aaagatcatc agaagacata tctgccgaat caaatacaac tgttttgatt
      601 ttggtactta cccaaaaccc tttttgctct ttttctacta tacggaaatt cagaatattt
      661 ccaaccgaat caaaagcacg gtaaacatca tccatcaaat cctgcggctc tattttcttt
      721 tccaattccg acaatccttg aaatatatcc aaagacacat cttcaaatag aaaaaaagga
      781 ggagttagaa gcggtttttc catgatctgt ccgtagattt tgattcccaa gggcgatgac
      841 gaccaattcc ctgtccaggc aaagtcttgc ccgtattatc cgtaactcga cgatgataat
      901 ggggaaattt tccaatagga tgacctgttc tattaccgaa aggggctatc cgcatattat
      961 tgccgatttt aatctcacgt ccatatttag caaaggaaac aacctttcct gcggcgccta
     1021 caccaccagg aattgcgcct aatccgccag caatagcaac atctctaaca gaagctggtc
     1081 tgcctgtcgt tgcataacta aaaccatgct gtgtccacat accaatggca gcaccaccca
     1141 agatagccaa tggaagaaac gccctctcag tctccttcat ctccttttga gaaagttccg
     1201 ccaactgcat cggcgcatct gcccgcgtat ggaatacttg gttttcaaat atctgattgt
     1261 ccatgctgtt tgccattgcg ggggcaatca tagacagcat cattacggct gcggtaattt
     1321 gttttttcat aataactcct ttggattaca aggttggaaa atcaaagccc tgcttagaac
     1381 gtatgttgca cacccaattt caataggtaa atcagattgc aaatccagca atttgaatat
     1441 tgtcattgtt ccgtgcaaaa gggattttta ttgatgagtt gtgtactggg tttcagcttg
     1501 gctttttaga taatcttttc acaagaacat acatcaatac tgtccaaaaa aaaagatttg
     1561 ataaatatat tgcaacatat tttattccac ctccatccga aacagaaacc aatatatttt
     1621 taattaatac atagccgatt attacattta ttattcctat aatatataag gatcttgcca
     1681 aaattttttt tgattttatt ttaggaatat ctcttatcca tgctaaaata cagcccaatg
     1741 tcgaaaagaa aataacagca aacactacct tacctcctct cacttccaac caaaccgata
     1801 gcaggtttgg ttggaagttg gtttatttat tatttaacgg cgaatgtcag tgttcttacc
     1861 cgtagaacct gcataaccag ttatgccacc agcaacagtc cccactgcta cacctcgcat
     1921 accattaatc agaattcttc ctattttttc gaatccacta tttcttccaa agatgcagca
     1981 aacgcaggtt gagcctagac ccaatctacg tttgccgccg catctgatcc ctattgtttc
     2041 ttatccttac atcttcctgc cttgtcaatc aaataaagac agaagactat acaaaaactg
     2101 accgacatac tgaaaatacc tatccccttc catgcaatct ccgcaccatt gacccaatag
     2161 atcagactga gaaacaacaa agccacagta taaatcaacg taaaaaatat tgcgtaaagc
     2221 acaataaagg gaacttttat ctcacggttg ttttttataa tatattcagc aacttgattt
     2281 ccgaatatac ctgataaaaa atatagtatt agatcatcca tttcgtttat cttctatgtt
     2341 ttcccattgc ggcgctagaa gactgattta atgctgcacc atttgcacga ataactgcat
     2401 taggaatagc atttccccct ttccaagcag aacgggcaaa a
//
LOCUS       DAWKST010000206         2440 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.206, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000206 DAWKST010000000
VERSION     DAWKST010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.206"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(425..2305)
                     /locus_tag="WJL87_001931"
     CDS             complement(425..2305)
                     /locus_tag="WJL87_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEZ7800327.1"
                     /translation="MNIFYEESGQFKIAAIIQKNDATYQVDTQHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFSAEAIAEEYFGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKISAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLALPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLDNGTKRVGIHIAAPS
                     LAVKPGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGAHRPAVSIYFDVDSE
                     FNTGEPTCKIEAVNISENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFAIAL
                     QKARGKYEPDRAPQYDYSIELDEEGNVSIVRRERGSPIDTLVSEMMILANSTWAQMLD
                     ENDLPGLFRVQPAGKVRMSTKSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLFQQSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
BASE COUNT          496 a          590 c          722 g          632 t
ORIGIN      
        1 atttccacct gcgcgggaat gacgattcag aagttgcccg aaacctaaaa aactgaaacc
       61 gaacgagccg gatttccgct ttcgcgggaa tgacggcgta taagttcccg tgcggacaga
      121 cctatattcc cacctgcgcg ggaatgacga ttcagaagtt acacgaaact caaaaaaaac
      181 gaaaccgaac gaaccggatt cccgctttta cgggaatgac ggcgcataag ttcccgtgcg
      241 gacagaccta gattcccacc tgcgtgggaa tgacgggatt ttagattgcg ggtatttatc
      301 gggaacggcg gcttggaagt tcattgaaac ggaaaaacaa cggaaaccca aaaaaccgga
      361 ttcccgactg tgggaatgat gagattcagg tttctgtttt tgccggagtt tgccgtatcg
      421 ggcttcagac ggcattgcct gccgttgtac ccgcgggtgc gactgccttg atgtagttga
      481 gcgagacaaa ctgcttctcg gcatccaatt cggtgatttt gaacaatgcc tgtgatttgg
      541 gcagcgcgtc aaacggaata ccggtcgcgc gcgttaccag cggcaggcct tcaatgcgga
      601 cgaggtcttc tttgaggatg gtggccgtca gctcgctcgt gccttgctgt tgcagataga
      661 cgaggctcca gtaggcttcc atttggcgtt ggaagtcggc gtaggctgta taggcggcat
      721 caaagtcgcg cagcgcggcg aagagttcgg catcgctttg ttggaaaagc ggctcggcgg
      781 tgtcgtctat caggctgatc agttgctttt ggttgatgta gtcggcggcg cggcgcagcg
      841 gcgaggtaaa ccagccgtaa tgctgcacgc ccattccgat gtgcggctcg gatttggtgc
      901 tcatacgcac tttgcctgcc ggttgcacgc ggaaaaggcc gggcaggtcg ttttcatcca
      961 acatttgcgc ccaagtgctg ttggcaagaa tcatcatctc gctgaccagc gtatcgatgg
     1021 gcgaaccgcg ttcgcggcgg acgatggata cgttgccttc ctcatccaat tcgatgctgt
     1081 aatcgtattg cggcgcacgg tcgggctcgt atttgccgcg tgctttttgc agggcgatgg
     1141 cgaattgata gaaccaaatc aggtcttgat ggtgggcgaa catcatttcg cccgcttcgt
     1201 ccaagcccgc ttcggcgttg aaatgcggtt cgatggcttg gatacgcagg ttttcggaga
     1261 tgttgaccgc ttcgattttg caggtcggtt cgccggtgtt gaactcgctg tccacatcga
     1321 aataaatgct gacggcaggg cggtgtgcgc ctgcatcaag gctgaacgcg gcaatccagt
     1381 tttcgggcag catcgtgatt ttgccgccgg ggaaataaac cgtgctcaag cgttccatga
     1441 tgtttttttc cattttgtcg cccggtttaa cggcaagtga cggcgcggcg atgtggatgc
     1501 cgacacgctt cgtgccgttg tccaagtcgg tcaggcttaa agcgtcgtcc acttcggtgg
     1561 tcgattcgtc gtcaatggaa aaggcggtaa cgtcggcctt gggcaggtcg ggcatttcgg
     1621 gaagggcaag gtcggggaag cctgtgcctt taggaaagta tttgatttca aacccgtctt
     1681 gcaggtattg cggaatggac gtaatgccac ccgttttttt cgccaattcg taggcggaga
     1741 ttttcagtgc gtcggcggct ttggtaaagg ctttgtaggt cagcgactgt ttgtcgggcg
     1801 cgtgcaggat ggttttcaaa tccgccgcga tttcagacgg catctcgccc cgttttaagg
     1861 cttccgccca agcgtcgatt tgcgcgtctt gctgtttttt gcgttcgatg gcggcaagcg
     1921 cttgtttcaa agtttcttcg ggcgcggctt tgaacacgcc tttggctttt ttgtagaaat
     1981 acatcggcgc ggcgtaaagc gcaatcaagg tcgccgccag ctcggttttg gtcggcgcat
     2041 gaccgaaata ttcttcggcg atggcttcgg cggaaaattc ttcttcgccg catacttccc
     2101 acaataaatc ggtgtcgatg tccgccgcct gtgcctgcgc gttttccaaa aacgccgcca
     2161 tatcgccgtc aaactcggca aagacattgt ttgccttcac tttggtgcgc ttgccgtgtt
     2221 gggtatcgac ttggtaggta gcgtcgtttt tttggatgat ggcggcgatt ttgaattggc
     2281 cggactcttc gtaaaaaata ttcatttttc ggatttttct gtggaaactc aagcgggcga
     2341 ttttagcaga ttaccgaaaa tgccgtctga aaaaaggttg ggagagggtt ggcgcgtctt
     2401 tgcggtgctt gcgttatagt ggattaacaa aaaccagtac
//
LOCUS       DAWKST010000207         2432 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.207, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000207 DAWKST010000000
VERSION     DAWKST010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.207"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(227..1861)
                     /gene="groL"
                     /locus_tag="WJL87_001932"
     CDS             complement(227..1861)
                     /gene="groL"
                     /locus_tag="WJL87_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ7800328.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGQVISEEVGLSLEKATLEDLGQAKRIEIGKENTTIID
                     GFGDAAQIEGRVAEIRQQIEVATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEDKPAMPDMGGMGGMGGMM"
     gene            complement(1954..2244)
                     /locus_tag="WJL87_001933"
     CDS             complement(1954..2244)
                     /locus_tag="WJL87_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ7800329.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
BASE COUNT          491 a          656 c          629 g          656 t
ORIGIN      
        1 ttcgcgggaa tgacggcgga aaggttgctg tttttccgat aaattcctgc cgctcttcgt
       61 ttttgggatg gcgggaaata aaacaaaagc gcgcgtatca aaaaataaca atgcaaagaa
      121 cggtgttggc aattttaagg tttgcgcctt agccgcactt attcgcaaaa aaaccgcacg
      181 gcgttgaccg tgcggcttgt tgtctgaagg cttcagacgg cattgcttac atcatgccgc
      241 ccataccacc catgccgccc atatcaggca tagccggttt gtcttcaggg atttcagcaa
      301 tcatgcagtc tgtggtcagc atcaggccgg cgatagacgc ggcgtgttgc agcgcggaac
      361 gggttacttt ggcggggtcg agtacgccca tttcgatcat gtcgccgtat tcgccgctgc
      421 cagcgttgta accatagttg cctttgcctt ccaacacttt gttcacaacc acgctgggtt
      481 cgccgcctgc gttggcaacg atttggcgca gcggagactc aacggcgcgc aagacgattt
      541 gtacgcctgc gtcttggtct gcattgccgg tgtgcaggtt ttccagagcg gcacgggcgc
      601 gcaacagggc tacgccgccg cctgcgacca cgccttcttc aacggcggca cgggtcgcgt
      661 gcagcgcgtc ttctacgcgg tctttcttct ctttcatttc cacttcggtt gcggcaccga
      721 ctttaatcac cgccacaccg ccggccaatt tggccacgcg ctcttgcagt ttttcacgat
      781 cgtagtcgct ggtggccact tcgatttgct gacggatttc cgccacacgg ccttcgattt
      841 gggcggcatc accgaagcca tcaataatgg tggtgttttc tttgccgatt tcaatgcgtt
      901 tggcttgacc caaatcttcc aaagtggctt tttccaaaga caagcccact tcttccgaaa
      961 tcacttggcc accggtcaaa atggcgatgt cttgcaacat ggctttgcga cggtcgccaa
     1021 agcccggtgc tttcacggcc acggttttca ggatgccgcg gatgttgttc accaccaaag
     1081 tcgccaaggc ttcgccttcc acgtcttcag cgataatcaa gagcggacgg ctggctttgg
     1141 ccacttgttc caaaacaggc agcaggtcgc ggatattgct gatttttttg tcgaacagca
     1201 atacaaacgg attgtccaag ccggcgattt gtttttccgc atcgttgatg aagtaaggag
     1261 acaggtagcc gcggtcgaat tgcatacctt caaccacgtc cagctcgttt tccaaagatt
     1321 tgccgtcttc aacggtaatc acgccttctt tgccgacttt ttccatcgct tcggcgataa
     1381 tcgcgccgac ttgttcgtcg gagttggcgg aaatagagcc gacttgggcg atttctttag
     1441 aagtatcgca aggtttggcg atgtttttca gctcgtcaac caaagcggca acggctttgt
     1501 cgataccgcg tttcaggtcg gtcggattca taccggcggt aacgtatttc ataccttcgg
     1561 caacgatgga ttgtgccaat acggtggcgg tagtcgtacc gtcgcccgcc acgtcgttgg
     1621 ttttggacgc gacttctttc accatttgcg cgcccatatt ttcaaacttg tctttcagtt
     1681 cgatttcttt ggcgacggtt acgccgtctt tggtgatgtg cgggccgccg aaagcgcggt
     1741 caacgactac gttgcggcct ttggggccca aggttacgcg cacggcgttt gccaaaatgt
     1801 tcacgccgtt taccattttt tggcggactt cattgccgaa ctgtacgtct tttgctgcca
     1861 tttcaattct ccaaaaatca ttaaaactgt ctgataaaac cgtttatgcc gtctgaaggc
     1921 ggtttgccgt ttcagacggc atcgtgtccg tatttatttt tcaacgatgc cgaaaatatc
     1981 ttcttcacgc attaccaaca gctcttcgcc gtcggctttg acggtttggc cgctgtattt
     2041 gccgaaaatg attttgtcgc cgactttgac atccagcgga cggcgcgcgc cgtctttgcc
     2101 gattttgccc gcgcccacgg cgatgacttc gcccatatcg ggtttttcgg cggccgcacc
     2161 cggcaaaacg atgcccgatg cggttttctc ttcagcttcc aagcgtttga cgacaacgcg
     2221 gtcgtgtaaa ggacggatgg tcatatttat gctccgataa aatgatttga aaacaatcat
     2281 ctgcccgaac ggttcggaca gattgaagtg gaaaccgggc tgccgcctgc cggcccgccc
     2341 gtataagtcg gcaaaattag ggtgtgtgcg ggtaaattca agtgaggcgg aaaaaaattt
     2401 atttccgccg ttttttatag tggactaaat tt
//
LOCUS       DAWKST010000208         2410 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.208, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000208 DAWKST010000000
VERSION     DAWKST010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.208"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(4..624)
                     /locus_tag="WJL87_001934"
     CDS             complement(4..624)
                     /locus_tag="WJL87_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7800330.1"
                     /translation="MTTHHVELRNVTKRFGAQKAVNQVDLVLKAGESVGLAGYNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIIVD"
     gene            complement(682..1914)
                     /locus_tag="WJL87_001935"
                     /pseudo
     CDS             complement(682..1914)
                     /locus_tag="WJL87_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712464.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            complement(1917..2342)
                     /locus_tag="WJL87_001936"
                     /pseudo
     CDS             complement(1917..2342)
                     /locus_tag="WJL87_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
BASE COUNT          520 a          626 c          660 g          604 t
ORIGIN      
        1 aatttaatcc acgataatgc ggtcggcgtg tccgtccaac tcggcaaggg cgtgggtgct
       61 gagcaatacg gtcgcgccgc gcccgttgag ttcgcgcacg acttcgtaaa acatttgtcg
      121 tgatgcaggg tcgaggccgg ttgtcggttc gtcaaacagc aggactttgg gctcgcccag
      181 cagggcttgt gccaaggcaa ggcgttggcg catcccttta gaataagtgc cgacgcggcg
      241 gtgtgccgcc tgtgaaatgc cgacgcgctc aagcagcccc cggttctgcg tgagcggctg
      301 ttttttaagt ttggcataaa aatccagcgt ttcgatgccg gtcagcgaag ggtgcagcgc
      361 aacggtttcg ggcaggtagc cgatttggct gcgaagccgc gcccccgctt tgctgccggt
      421 acgttcgccc aaaagcatca cttcgccttc ggtcggggta atcagcccga gtatcagctt
      481 cataatggtg gacttgcccg cgccgttgta tccggcaagc ccgacgcttt ctcctgcctt
      541 caaaaccaaa tcgacttggt tgacggcttt ttgcgccccg aaccgtttgg tgacattcct
      601 caattcgaca tgatgtgtgg tcatggttgt tccttttgtg ttttcagacg gcatttggat
      661 ccggatgccg tctgaagcag actagttcaa agaaccgttt tccgccctgc cccattccga
      721 ctgccgtgtt tcggcttctt tgagcaactc gtccttcatt gcctgatagc gggtttgaat
      781 tttggggaca taaggcttca tcagcggttt gctgtccacc acgccgccgg gcagaacggc
      841 gggaaactgc gcctgcgccc atttgacgat gctgattgcg agactgttca tcaagaggcg
      901 caatacgggc gcgcgccaga tgatttggtc gatgatgccg ttgggccggt acgcgctgtc
      961 gccgaagccg tcgccgttca aatcgaacgc actgttgtcg ctccaatagt tgccgtgtcc
     1021 gccctcgctc cagtcgagaa agcgcgtgct gacgtatttg acctggcttt cgttgttgat
     1081 aaaggaattg tcgtgcaggg acgtgccttc gatggcggcg gtaaagtgta tgccgatttg
     1141 gcagttttca aaatgattgg cggacagttt atcgtagttg gcattgtagg caaaaacgca
     1201 cttgcccgct ttgttgataa tgttgtcgtg aatatcggaa tagttgacat agttgagcat
     1261 gatgccttgg tcgcggctgc cgacggcgat attgtcaaac actttgagcc gctcggaaaa
     1321 catcagcaca tagcccatat tgttgcccac ggaaatattg ccgctgattt cgctgtcgtt
     1381 ggtgtacata tagtggacgg cgaaacgcaa atcgctgaag cggttgtttt tataggtgtt
     1441 gtgcgtgctg gtattggaaa aaatgccgtc gcggcctttg gaaatatcgt tgccgacgac
     1501 ctgcgcgccg ggcgcgttcc aaacggtaac gccgttgcct cgctcgttca cgcgcaaagt
     1561 cgcatcgcca acaattttgt tctcgcgcac catcgcatcg gcagaaccat gcagatatac
     1621 gccgaccgaa ttatccaaaa tattgttgtg ttcaatcagg gcgcgcgggg cggtttcttc
     1681 gagataaata ccggcatcca ttgcaggcag gctcataccg gaacgggtaa cggtcaggtt
     1741 gcggagcgtt acgtccggcg catgcacagc tatcgtacgt ccgctcctgt ctccttcgat
     1801 ggttgcagaa cggtcagcag gcccttcaat cgtaatcggt ttgtcgatat aaagtttggt
     1861 ttgatatacg ccggaagcca gtttgagcgt atcgcccgca tgggcgcggg caaatgcggg
     1921 ataccgatca gcataatcgg ttcgtgatga tcgaccagtt tcgctccggc tatcgtgccg
     1981 tcatttgcca aagccatcag cgtatcgatc ggtttgttcg aataaccgcg cgtattgacc
     2041 gcatcggtcg tgatatagac caagcccaac tgctcatcgc ctttgtaaac gcgggcaacc
     2101 ataggcttgc cttccggctt gccgtaacgg tccgcacccg gaaaaatttc cgaaggctgt
     2161 attttcgcca gaaaatcagg cagacgctcc gcataagcgg gcagtgccgc cgtcaaaaag
     2221 acaaaggcgg taaaaaacac tgttatccgc gcccgaaacg cggacatacg tttgattgaa
     2281 aaacaggaca tgactatccc cgatataccg atactcttta atattgatat tatatgaatc
     2341 atctatcggc gattttttga tgcaaattaa atttacaaat cttaaaacca atccgcatac
     2401 gggataatat
//
LOCUS       DAWKST010000209         2376 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.209, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000209 DAWKST010000000
VERSION     DAWKST010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.209"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..332
                     /locus_tag="WJL87_001937"
     CDS             <1..332
                     /locus_tag="WJL87_001937"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800331.1"
                     /translation="NPHHRHSRAGGNPKTQRNRNLSEKTETPPTVIPAKAGIQQHNAA
                     GIYRKKQKPPPTVIPAKAGIQQPKNHRNLSAKTETPTAVIPAKAGIQTRRHGNLPNKT
                     VSLDSTS"
     gene            477..2246
                     /locus_tag="WJL87_001938"
     CDS             477..2246
                     /locus_tag="WJL87_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HEZ7800332.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVATAVLATLLS
                     ATVQANATDTDEDEELESVVRSALVLQFMIDKEGNGEIESTGDIGWSIYYDDHNTLHG
                     ATVTLKAGDNLKIKQSGKDFTYSLKKELKDLTSVETEKLSFGANGNKVNITSDTKGLN
                     FAKETAGTNGDPTVHLNGIGSTLTDTLAGSSASHVDAGNQSTHYTRAASIKDVLNAGW
                     NIKGVKTGSTTGQSENVDFVRTYDTVEFLSADTKTTTVNVESKDNGKRTEVKIGAKTS
                     VIKEKDGKLVTGKGKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTTTANGQTGQAD
                     KFETVTSGTKVTFASGNGTTATVSKDDQGNITVKYDVNVGDALNVNQLQNSGWNLDSK
                     AVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEITRNGKNIDIATSMTPQFSSVSLG
                     AGADAPTLSVDDEGALNVGSKDANKPVRITNVAPGVKEGDVTNVAQLKGVAQNLNNRI
                     DNVDGNARAGIAQAIATAGLAQAYLPGKSMMAIGGGTYRGEAGYAIGYSSISDTGNWV
                     IKGTASGNSRGHFGTSASVGYQW"
BASE COUNT          729 a          592 c          583 g          472 t
ORIGIN      
        1 gaaaccctca ccaccgtcat tcccgcgcag gcgggaatcc aaaaacgcaa cgcaacagga
       61 atttatcaga aaaaacagaa accccaccga ccgtcattcc cgcaaaagcg ggaatccagc
      121 aacacaacgc agcaggaatt tatcggaaaa aacagaaacc cccaccgacc gtcattcccg
      181 caaaagcggg aatccagcaa ccgaaaaacc acaggaatct atcagcaaaa acagaaaccc
      241 ccaccgccgt cattcccgca aaagcgggaa tccagacccg tcggcacgga aacttaccga
      301 ataaaacagt ttccttagat tccacgtcct agattcccgc cttcgcggga atgacgagat
      361 tttaagttgg gggaatttat cagaaaaccc ccaaccccca aaaaccgggc ggatgccgca
      421 ccatccgccc ccaaaccccg atttaaccat tcaaacaaac caaaagaaaa aacaaaatga
      481 acaaaatata ccgcatcatt tggaatagtg ccctcaatgc ctgggtagtc gtatccgagc
      541 tcacacgcaa ccacaccaaa cgcgcctccg caaccgtggc gaccgccgta ttggcgacac
      601 tgctgtccgc aacggttcag gcgaatgcta ccgataccga tgaagatgaa gagttagaat
      661 ccgtagtacg ctctgctctg gtgttgcaat tcatgatcga taaagaaggc aatggagaaa
      721 tcgaatctac aggagatata ggttggagta tatattacga cgatcacaac actctacacg
      781 gcgcaaccgt taccctcaaa gccggcgaca acctgaaaat caaacaaagc ggcaaagact
      841 tcacctactc gctgaaaaaa gagctgaaag acctgaccag tgttgaaact gaaaaattat
      901 cgtttggcgc aaacggtaat aaagtcaaca tcacaagcga caccaaaggc ttgaattttg
      961 cgaaagaaac ggctgggacg aacggcgacc ccacggttca tctgaacggt atcggttcga
     1021 ctttgaccga tacgcttgcg ggttcttctg cttctcacgt tgatgcgggt aaccaaagta
     1081 cacattacac tcgtgcagca agtattaagg atgtgttgaa tgcgggttgg aatattaagg
     1141 gtgttaaaac tggctcaaca actggtcaat cagaaaatgt cgatttcgtc cgcacttacg
     1201 acacagtcga gttcttgagc gcagatacga aaacaacgac tgttaatgtg gaaagcaaag
     1261 acaacggcaa gagaaccgaa gttaaaatcg gtgcgaagac ttctgttatt aaagaaaaag
     1321 acggtaagtt ggttactggt aaaggcaaag gcgagaatgg ttcttctaca gacgaaggcg
     1381 aaggcttagt gactgcaaaa gaagtgattg atgcagtaaa caaggctggt tggagaatga
     1441 aaacaacaac cgctaatggt caaacaggtc aagctgacaa gtttgaaacc gttacatcag
     1501 gcacaaaagt aacctttgct agtggtaatg gtacaactgc gactgtaagt aaagatgatc
     1561 aaggcaacat cactgttaag tatgatgtaa atgtcggcga tgccctaaac gtcaatcagc
     1621 tgcaaaacag cggttggaat ttggattcca aagcggttgc aggttcttcg ggcaaagtca
     1681 tcagcggcaa tgtttcgccg agcaagggaa agatggatga aaccgtcaac attaatgccg
     1741 gcaacaacat cgagattacc cgcaacggca aaaatatcga catcgccact tcgatgaccc
     1801 cgcaattttc cagcgtttcg ctcggcgcgg gggcggatgc gcccacttta agcgtggatg
     1861 acgagggcgc gttgaatgtc ggcagcaagg atgccaacaa acccgtccgc attaccaatg
     1921 tcgccccggg cgttaaagag ggggatgtta caaacgtcgc acaacttaaa ggtgtggcgc
     1981 aaaacttgaa caaccgcatc gacaatgtgg acggcaacgc gcgcgcgggt atcgcccaag
     2041 cgattgcaac cgcaggtttg gctcaggcct atttgcccgg caagagtatg atggcgatcg
     2101 gcggcggtac ttatcgcggc gaagccggtt acgccatcgg ctactcgagc atttctgaca
     2161 ctgggaattg ggttatcaag ggcacggctt ccggcaattc gcgcggtcat ttcggtactt
     2221 ccgcatctgt cggttatcag tggtaagggc tttttcgcct gtctgctgtt ggggcaggcg
     2281 gaaggtttga agggaagggt ggcgatttgc cgcctgagac ctttgcaaaa ttccccaaaa
     2341 tcccctaaat tcccaccaag acatttaggg gatttt
//
LOCUS       DAWKST010000210         2338 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.210, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000210 DAWKST010000000
VERSION     DAWKST010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.210"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            43..2256
                     /locus_tag="WJL87_001939"
     CDS             43..2256
                     /locus_tag="WJL87_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ7800333.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPDNDKKLVLAARSLA
                     EAHYPADAVTPYGEPLSDHFLGAAQMVDELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNVPDNPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLASLGDVKGVLSVTRL"
BASE COUNT          598 a          755 c          566 g          419 t
ORIGIN      
        1 ctgttacaat aaactcgttt ttatcggaac gaaagaccca tcatgaccgc catcagcccg
       61 attcaagaca cgcaaagcgc gactctgcaa gaattgcgcg aatggttcga cagctactgc
      121 gccgcgctgc cggacaacga taaaaaactt gtcttagccg cccgttcgct ggcggaagca
      181 cattaccccg ccgatgccgt cacgccgtat ggcgagccgt tgtccgacca cttcctcggc
      241 gcggcacaaa tggtcgacga actcgacctg ctgcccgatg ccgtcgccgc caccctgctt
      301 gccgacatcg gacgctacgt ccccgattgg aatctattgg tttccgaacg ctgcaacagc
      361 accgtcgccg agctggtcaa aggtgtggac gaagtgcaga aactcaccca cttcgcccgg
      421 gtggacagcc tcgccacgcc agaagaacgc gcccagcagg cagaaactat gcggaaaatg
      481 ctgctggcga tggttaccga catccgcgtc gtgttaatca aactggcgat gcgtacgcgc
      541 accctgcaat tcttaagcaa cgttcccgac aatcctgaaa agcgcgccgt tgctaaagaa
      601 accctcgaca tttttgcccc actcgccaac cgcttgggcg tgtggcaact caaatggcag
      661 ctcgaagatt tgggcttccg tcatcaagag ccggaaaaat accgcgaaat cgccctgctt
      721 ttggacgaaa aacgcaccga gcgcctcgaa tacatcgaaa acttcctcaa tatcctgcgt
      781 acggaactca aaaaatacaa tatccacttt gaagtcgccg gccgcccgaa acacatctac
      841 tccatttaca aaaaaatggt gaagaaaaaa ctcagcttcg acggcctctt tgacatccgc
      901 gccgtgcgga ttctggttga taccgtcccc gagtgttaca ccacgctggg cattgtccac
      961 agcctttggc agcctatccc cggcgagttc gacgactaca tcgccaaccc gaaaggcaac
     1021 ggctataaaa gtttgcacac cgtcatcgtc ggtcccgaag acaaaggtgt ggaagtgcaa
     1081 atccgcacct tcgatatgca ccaattcaac gaattcggtg tcgccgccca ctggcgttac
     1141 aaagaaggcg gcaaaggcga ttccgcctac gagcaaaaaa tcgcctggtt gcgccaactt
     1201 ttggactggc gcgaaaatat ggcggaaagc ggcaaggaag acctcgccgc cgccttcaaa
     1261 accgagcttt tcaacgacac gatttatgtc ctgaccccgc acggcaaagt cctctccctg
     1321 cccacgggcg cgacccccat cgacttcgcc tacgccctgc acagcagcat cggcgaccgt
     1381 tgccgcggtg cgaaagtcga agggcagatt gtgccgctgt ccaccccgct cgaaaacgga
     1441 cagcgcgtcg aaatcattac cgccaaagaa gggcatcctt ccgtcaactg gctttacgaa
     1501 ggctgggtca aatccaacaa ggcaatcggc aaaatccgcg cctacatccg ccagcaaaac
     1561 gccgacaccg tgcgcgaaga aggccgcgtc caactcgaca aacagcttgc caaactcacg
     1621 cccaaaccca acctgcaaga gcttgccgaa aatctcggct acaaaaagcc agaagacctc
     1681 tacaccgccg tcggacaagg cgaaatttcc aaccgcgcca tccaaaaagc ctgcggcacg
     1741 ctgaacgaac cgccgcccgt acccgtcagc gaaaccacca tcgtcaaaca gtccaaaatc
     1801 aaaaaaggcg gcaaaaacgg cgtgctcatc gacggcgaag acggtctgat gaccacgctt
     1861 gccaaatgct gcaaacccgc gccgcccgac gacattgtcg gcttcgttac ccgcgatcgc
     1921 ggcatttcgg tacaccgcaa aacctgcccc tctttccgac acctcgccga acacgcgccc
     1981 gaaaaagtac tggacgcaag ttgggcggcg ttgcaggaag gacaagtgtt cgccgtcgat
     2041 atcgaaatcc gcgcccaaga ccgctccggg cttttgcgcg acgtatccga cgcgctcgcc
     2101 cgccacaaac tcaacgttac cgccgtgcaa acccagtccc gcgacttgga agccagtatg
     2161 cggttcacgc tcgaagtcaa acaggtcaac gacctcccgc gcgtcctcgc cagcctcggc
     2221 gacgtcaaag gcgtattgag cgttacccgg ctttaaatac aaaaatgccg tctgaaagcc
     2281 gtataacgct tcagacggca ttttgattga cggggtttgc tattttttgt tgcatagt
//
LOCUS       DAWKST010000211         2334 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.211, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000211 DAWKST010000000
VERSION     DAWKST010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.211"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(80..1489)
                     /gene="dacB"
                     /locus_tag="WJL87_001940"
     CDS             complement(80..1489)
                     /gene="dacB"
                     /locus_tag="WJL87_001940"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ7800334.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGISDTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDIADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVAKNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1654..2220
                     /locus_tag="WJL87_001941"
     CDS             1654..2220
                     /locus_tag="WJL87_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ7800335.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTVFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          538 a          565 c          668 g          563 t
ORIGIN      
        1 ttaaaacaat tagtaaaacc gagtttatcc tagttatcta ggacagcccc ttaaatttaa
       61 tccactatat tttcctgttt caggctcggc gttctttgca catcagtttc gcatccagcc
      121 agccgtcgcc gccggagatg atgtttttgg caacgaagtt gtccaagtcg ggcagcaggg
      181 aaacggcgcg gccgctgttg atgatgacga ccaccgccat cggtttgtcg cccagccaat
      241 aacctgcaag ggcgcggaca ttgttgagcg tgccggtttt taagcgcaac agcccgccgc
      301 tttgtttgaa gcggttgcgt aaagttccgt ctgtgccggc gatgggcagc gtgtcgatga
      361 aatcttgtgc aaacgggctg aaataagccg tttccaacat ttgcgccatc attctcgccg
      421 ttaccctttc ttttctggac agacctgaac cgttttccaa aaccaaatcc gcaatatcga
      481 tgcccgacac ggcaagttcg cgccggacgg cagacgccgc ctgttcggaa acggcgggca
      541 gtttgccgtc gccgccgagt ttgaggaaga cggaacgcgc aattagattg tccgaacgct
      601 tgttcatgtc cgtcaaaatt tccttcatcg gctttgagtg tgcaacggca agcgtctgcg
      661 cgccttccgg cgtgtcggat atgccgatgc cgtctgaaat ccgtccgccg ccgagcagcc
      721 agtggttggt aaaactttgc cggatcagtt cgtcaagcgc gaacatccgg acaccgacag
      781 gcttgcccaa acagctctcg ggaatattgc cgcgcaattt cagcgtattg tccgaaaaag
      841 atgcacgcat cagttttttg atcgaagggc aggcagcttg ggaggcggta attttcaagt
      901 tgttttgggc gaaaatatgc ggcaaaggcg gatcggtgag gatgtcggta ctgtcggcgg
      961 cattgcgttc ggcgcgcacc ataaccatac cggcagacag catagttgga ttggggggcg
     1021 tcataaacgg cgaaccgctg tcggcttcga aatcgtcggg gctgccgact tcgccccaca
     1081 ggctgtggtc gagcatcagg tgtcccgtga tattgcgtat gccttgttcg cgcaactggc
     1141 gttggacggc aagcaggttt tcctgattga aaacggggtc gccgctgccc gcccaataca
     1201 ggtttccgtc aagcgtgccg tcgtttaccg taccgttgct tttaaactcg gtcgcccagc
     1261 ggtaattgct gccgaaggtt ttgaaggcgg caaacgcggt aacgagtttc attgtggagg
     1321 cggggttgac ggggacatcc gagcggtggt caatgatgac ttttccgctg tcaagctctt
     1381 ggacatatac ggcgatttcg ttttgcggaa tgcgacctgt atcgagcgcg tgtgcggcga
     1441 gggaggcgag aagcagcagc agggaggccg ctgttttggg gaaattcata ggtaaatctg
     1501 ttacataaaa aaagcaatta taaggcaaag ccggataagt gggaacgctg tggcggcggg
     1561 ccggcttgtt tgcaggggaa atcacatata taataatcgc tatcattatg aaatgattga
     1621 gacgcacaaa cttaatattc aggaggaatg attatggcta agaaaatcag tattttggtg
     1681 ggcagcctgc gccgtgcttc ttttgcgcgc aaagtggcat tgaatgcggc ggagatgttc
     1741 cccgaaggct ggcaggcgga aatcgtcgaa atcggacatc tgccgcttta caatttcgat
     1801 tatgacgacc ctgcggtgga agatgtgccg ctgcccgaaa gctacacggt tttccgcgaa
     1861 acgattaagg cttcggacgg cattttgttc gttacgtccg aaaataaccg caccattccc
     1921 gcctgtttga aaaatgcggt ggacatcggc tcgaaaccga atgccgacgt ggcgtggaaa
     1981 aacaaaccgg ccggcatcat cagccattcc gtcggcaaga tgggcggtta cagttcgcaa
     2041 aaaaacctgc gccttgcgct gtcttatttc gatatgcccg taaccggaca gccggaagtg
     2101 tttttgggca attcgccgac actgtttgat gaaaacggca aattgatcga ctcggcaagg
     2161 gattttgttc agtcatatat taatcagttt gtcggtttga ttgaaagaaa tgccaaataa
     2221 acaaacaagt cgaaaggccg caaaaccggt tcgggttttg cggctttttt atatgggctg
     2281 ggcagcgtca aggtttgccg tttgccggat aatgcgcggc aaatgaccgg tgtg
//
LOCUS       DAWKST010000212         2329 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.212, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000212 DAWKST010000000
VERSION     DAWKST010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.212"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(213..326)
                     /locus_tag="WJL87_001942"
                     /pseudo
     CDS             complement(213..326)
                     /locus_tag="WJL87_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            466..541
                     /locus_tag="WJL87_001943"
     tRNA            466..541
                     /locus_tag="WJL87_001943"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:500..502,aa:Glu,seq:ttc)
     gene            926..1091
                     /locus_tag="WJL87_001944"
                     /pseudo
     CDS             926..1091
                     /locus_tag="WJL87_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
     gene            complement(1141..1947)
                     /gene="thiD"
                     /locus_tag="WJL87_001945"
     CDS             complement(1141..1947)
                     /gene="thiD"
                     /locus_tag="WJL87_001945"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ7800336.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGSDVCKAVRTAKSYITAAISNPLEIGAGHGPVNHWAYRD"
BASE COUNT          552 a          593 c          603 g          581 t
ORIGIN      
        1 aagttgcact tcaaatgcga atccgccatt atctgaaagt ttgaggttct agattcccgc
       61 tttcgcggga atgacgaaaa gtggcgggaa tgacgaagtt tcagacggca tcgcccgcct
      121 gttttgatat ggcggcaccc cccccccgac aaaaaaaaac aatccggaac gcatctgacc
      181 gttccggctt gttttcaggc gaatccgccg catcagaaca tactgcgcac gcccatattg
      241 acctgccaag tctggcgcat cgtgtgcatc gaagaccttt gcgcctcaaa ataaagctgc
      301 cttccgttgt cggcattacc acgcaaaaaa atgaattgct tgatattcca atgtttttta
      361 tatgttttta tattgtgatg cgatcagaca aacgcccccc tgacatttgt ttagacggca
      421 tcgtattgct aaatttctat aagtatgtat aatgtccgtt tccacgcgcc catcgtctag
      481 aggcctagga cactgccctt tcacggcggc aaccggggtt cgaatccccg tgggcgtgcc
      541 aattcaaaaa cctgcttgtt tcaagcaggt ttttttatta tgaatcgtca ttcccgcacc
      601 cttcgtcatt cccgcgaaag cgggaatctg gagcgcagga ttaaagaaac cgttttatcc
      661 gataagcttc cgtgccgaca ggtctggatt cccgcctgcg cgggaaggac ggcagagggt
      721 ggacgatgcc gtctaaagcc tgacaaggca tttgatgccg tctgaaactt cgtcattccc
      781 gcacccttcg tcattcccgc aaaagcggga atctagagcg tagggttgaa gaaaccgttt
      841 tatccaataa gtttccgtgc cgacaggtct ggattcccac tttcgtggga atgacgagtc
      901 ttttataacc tgagaccttt gcaaaatgcc tttccctttt aaatttaaag aggggggagc
      961 tgaaggatta ttatcgggat tgggagctgg tcgaatataa agaagaattg ggcgcgatgc
     1021 acgccaaaga cgaaaacggc aatccgatac ggtttaaatt tgtaaccatg ctggcgaaaa
     1081 agcctgaata atcctcaaaa atgccgtctg aacattttgt ttcagacggc atttttacgt
     1141 ctagtcccga tacgcccaat gattgaccgg gccgtgtccc gcgccgattt ccaaagggtt
     1201 tgagattgcc gccgtaatgt aggacttggc agtccgtacg gctttgcaaa cgtccgagcc
     1261 ttttgccaac tcggcggtga tgcaggcgga aaacgtgcag cccgtgccgt gcgtgtgggc
     1321 ggttggaaag cgcgggctgt ccaattccag cgtttcattt tgcgtaaaca gccaatccgt
     1381 gcagcgtccg cttgtgctgc cgttcaaatg tccgcctttg ataacgacat ttttgacacc
     1441 gtaatcaagc aggatttttg ccgcacgttc cgcatcttta cggttttcga tatgcacgcc
     1501 ggtcagggct tccgcctcgg gcaggttggg ggtcaataca tccgtatcgg gaagcagcag
     1561 gcgcgtcagt gccgcaacgg cggaatcctg caacagcggc gcaccgcctt tggcaatcat
     1621 cacagggtcg agtacgcgcc tgccgaagtc gcagtgtttc agcttgtcgg caacgcattc
     1681 gatgatttcc gccgtgccga gcattcctat tttgtaggcg cggatgtcaa agtcttccct
     1741 gattgcttgg atttgtgcgg tgatggtttc ggtcgggacg agatgaaccg ccgacacgcc
     1801 caaggtattt tgcgcggtaa cggcggtgat gacgcacgtt ccgaacacgc cgcgcatctg
     1861 aaacgttttc aaatccgcct gaatgcccgc accgccgccc gaatccgaac cggcgatggt
     1921 aagcgtttgc acaaaagagc ttttcatttg ttcctcctta atattgtgtc cgataaaaag
     1981 ggctaatgcc tgtaaaaaag cacggcgatg ccgtctgaaa accgcagcgc ggggtttgtg
     2041 gtgcattata tgccgtattg caggacaagg cacagggcag ggcggcgggg tgcatcgtag
     2101 caactgtatt tttgcccgac ggggtgaaaa atacagttgc tacacccact gataatgaaa
     2161 aaagagagca atgccgtctg aaccgcgtgt tgcggcgagc ataaaccggc acgctttatt
     2221 cgtcattccc acaaaaacag aaacctaaaa ttccgtcatt cctacgaaag cgggaatcca
     2281 gtgtgttcgg tttcagttgt ttttgggttt cgggtgattt ccaaatcga
//
LOCUS       DAWKST010000213         2309 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.213, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000213 DAWKST010000000
VERSION     DAWKST010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.213"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..569)
                     /locus_tag="WJL87_001946"
     CDS             complement(<1..569)
                     /locus_tag="WJL87_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ7800337.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTSKGQGKPAGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKR"
     gene            731..2137
                     /gene="dnaB"
                     /locus_tag="WJL87_001947"
     CDS             731..2137
                     /gene="dnaB"
                     /locus_tag="WJL87_001947"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ7800338.1"
                     /translation="MNDYTAMPSEDREIGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          564 a          596 c          621 g          528 t
ORIGIN      
        1 cgtttggcga cttcgtccca gttgacgatt tcccaaaaac ctttcaggta gttggggcgg
       61 ctgttgcggt agtcgatgta gtaggcgtgt tcccacacgt cgcaggtcag cagcggcgtg
      121 ttttcagtgg tcagcggcgt agcggcgttg gaagtagaaa ccaaatccaa tccgccggca
      181 ggggttttta ccagccacgc ccaaccggag ccgaaagtac cggccgcgca ggcattgaac
      241 gcttcttgga atttctcgaa gctgccccat ttcgcgtcga tggcggcggc cagttcgccg
      301 gcaggtttgc cttgaccttt ggacgtgaaa cccagccagt agaaggtgtg gttccaagtt
      361 tgtgccgcgt tgttgaacac gccgcccgaa gattttttca caatctcttc caaaggcagg
      421 ttttcaaatt cggtgccttt gatttgattg ttcaggttgg tgatgtaggt ttgatggtgt
      481 ttgccgtagt ggaattccaa agtctctttg ctcagatgcg gggacaatgc gtccagttca
      541 taaggcagtt gcggcagctt atgttccatt ttgtgctcct aatattgttt taaatgttgt
      601 attttggcag tgtgctgcaa atagctcagc agcgcacata ttttacctgt tttgagggat
      661 ggaaaccaat taaacctgct ttacgctata atagaagatt gcaatttcgg cacgacagat
      721 aggatgtacc atgaacgatt acacagccat gccgtctgaa gaccgtgaga tcggcgcatt
      781 gtcgctgcct ccacattcga tggaggcgga acaatccgtt ttgggcgggt tgatgctgga
      841 aaatccggca tgggacagga ttgccgatgt ggtttcggga gaggatttct accggcatga
      901 acaccgcctg attttccgat ccattgccaa attgattaat gagagccgtc ccgccgatgt
      961 gattacggtt caggaagatt tgcagcggaa cgaagaattg gaagcggcag gcggattcga
     1021 atatctgatt acgctggcgc aaaacacccc gtctgccgcc aacatccgcc gctacgccga
     1081 aatcgtgcgc gagcgttcca tcatgcgcca actcgccgaa gtggggacgg aaatcgcccg
     1141 cagcgcatac aatccgcaag gcagggacgc ggggcagctt ttggacgagg cggaaaacaa
     1201 agtattccaa atcgccgaaa gcacggccaa atccaagcag ggctttttgg agatgcccga
     1261 tttgctgaaa gaagtcgtac agcgcatcga tatgctctac tcgcgcgaca atcccgatga
     1321 agttaccggc gtgccgacgg ggttcatcga cctcgacaaa aaaacctcgg gtctgcaacc
     1381 cggcgacctg attatcgtcg ccggtcgtcc ttctatgggt aagaccgcct tttccatcaa
     1441 tattgccgaa cacgttgccg tagaaggcaa gctgcccgtt gccgtctttt cgatggagat
     1501 gggcggtgcg caactggtca tgcgtatgct cggctcggtc ggacggctgg atcaaagcgt
     1561 tttgaaaacc ggcaggctcg aagacgaaca ctggggtcgc ctgaacgaag cagtcgtcaa
     1621 actctccgac gcgcccgtgt acatcgacga gaccccgggt ttgactgcgc tcgaactgcg
     1681 cgcccgcgcc cgccgtctcg cccgtcaatt taacaataag ctgggattga tcgtcatcga
     1741 ctacctgcaa ctgatggcgg gatccggccg ttccgacaac cgagcttcag agctgggcga
     1801 aatttcacgt tcgctcaaag cgttggcgaa agaattgcaa gtccccatca tcgccctgtc
     1861 gcaactgagc cgcacggtcg aatcgcgtac cgacaaacgc cccatgatgt ccgaccttcg
     1921 cgagtccggc gcaatcgagc aggatgccga cctgattatg ttcatgtacc gcgacgaata
     1981 ctacaaccag gactcaccca tgaaaggcct tgccgaatgt atcatcggca aacaccgcaa
     2041 cggtcccgtc ggtaaaatct tcctcacatg gacgggacaa ttcaccaaat tcgacaatgc
     2101 tgcctatatt cccgaggagg caaagataga ggattaaatg gctatataaa aatttattag
     2161 gcgaaatcag gcaaaatcgt ttaaaatcat gctgagagat tgccctaaaa aataaaacgc
     2221 ggtcttgagg catttttgca ttcagcccgc atataattga aaatatagtg gattaacaaa
     2281 aatcaggaca aggcaacgaa gccgcagac
//
LOCUS       DAWKST010000214         2155 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.214, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000214 DAWKST010000000
VERSION     DAWKST010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.214"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            46..756
                     /locus_tag="WJL87_001948"
     CDS             46..756
                     /locus_tag="WJL87_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ7800339.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFGDADYFKVPSNGEAAHS
                     EKAFDAEKIKAFLHERGIASCPTVLKAVRQHGNASDKFLKVVETVDLAAVLAKIPECR
                     HICTTGGKATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKK
                     AAAYRAFFEMAGLCEKQL"
     gene            941..2143
                     /gene="trpB"
                     /locus_tag="WJL87_001949"
     CDS             941..2143
                     /gene="trpB"
                     /locus_tag="WJL87_001949"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ7800340.1"
                     /translation="MKNYHAPDEKGFFGEHGGLFVSETLIPALQELADAYKAAKNDPE
                     FWEAFRHDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVESGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
BASE COUNT          504 a          559 c          633 g          459 t
ORIGIN      
        1 tccgtttgat gtcggcattg taaccgaaaa cggcagggcg tgataatgct gtttgaaggc
       61 ttgccgtgtt tggcggtttg gtgcaaaaac cggctgtctg ccgttttgcc tgttggagga
      121 ttgaacgtgt ctgaaaatct gcttgaaatc gaaacccatc ccttcgattc cgtgttgccg
      181 ccgaaggctg cggtcatgat gatggggacg tttccgccca aggaagacaa acgcgcgatg
      241 cagtttcatt atccgaattt ccaaaacgat atgtggcgcg tttacgggct ggtgtttttc
      301 ggcgatgcgg attatttcaa agtgccgtcc aacggggagg ctgcgcactc tgaaaaagcg
      361 tttgatgccg agaaaatcaa ggcgtttttg cacgaacggg ggattgcgtc ctgtccgacc
      421 gttttgaagg cggtacgtca gcacggcaat gcgtccgaca agtttttaaa ggtagttgaa
      481 accgtcgatt tggcggcggt gttggcaaaa atacccgagt gccgccatat ttgtacgaca
      541 ggcggcaagg cgacggaaat cctgctcgat attcaaggtg gcggcatcaa aatgccgaaa
      601 acgggcgaaa ccgtgccgtt tccgtttgcc ggacgggatt tgacgctgac gcgcctgcct
      661 tcgacttcgc gtgcctatcc tttgagtttg gcgaaaaagg cggcggcgta tcgggcgttt
      721 ttcgaaatgg cgggtttgtg tgaaaaacag ttataattgc cgacaatttc ccgttcggac
      781 ggcatgtttg aaaaatatag tggattaaca aaaatcagga caaggcggcg aagccgcaga
      841 cagtacaaat agtacggcaa ggcggggcaa cgccgtaccg gtttttgtta atccactata
      901 aattgccgtc tgaaaatttg aagcacaagg acgaaatctg atgaagaact accacgcgcc
      961 cgacgagaag ggctttttcg gcgaacacgg cgggctgttc gtgtccgaga ccctgattcc
     1021 ggctttgcaa gagctggcgg atgcctataa ggcagcgaaa aacgatcctg aattttggga
     1081 agcgttccgc catgatttga aacattatgt cggcaggccc agccccgttt accacgccgc
     1141 gcggttgtcc gaacatctgg gcggtgcgca aatctggttg aagcgcgaag acttgaacca
     1201 caccggcgcg cacaaagtca acaacaccat cggtcaggca ctgttggcaa aacgcatggg
     1261 taaaaaacgc gtcatcgccg aaaccggcgc gggtcagcac ggcgtggcga gtgccaccgt
     1321 tgccgcacgc ttcggtatga cttgcgacgt gtatatgggc gcggacgaca tccaacgcca
     1381 aatgcccaac gtgttccgta tgaaattatt gggtgcgaac gtggttggtg tagaaagcgg
     1441 cagccgcacg ctgaaagacg cgatgaacga agccatgcgc gaatgggtcg cccgcgtgga
     1501 cgacacgttc tacatcatcg gtaccgccgc cggccccgcg ccgtatcccg aaatggtgcg
     1561 cgatttccaa tgcgtgattg gcaacgaagc taaagcgcag atgcaggaag ccatcggcag
     1621 acagcccgac gttgccgttg cctgcgtggg cggcggatcg aacgccatcg gtttgttcca
     1681 cccgtatatc ggcgaagaaa acgtgcgcct cgtcggcgtg gaggctggcg gtttgggcgt
     1741 gaacaccccc gatcacgccg cgccgattac ttcgggcgca ccgattggcg tattgcacgg
     1801 tttccgcagc tatctgatgc aggacgaaaa cggtcaggtt ttgggtacgc actctgtttc
     1861 cgcaggcttg gattaccccg gcatcggccc ggaacacagc catctgcacg acatcaagcg
     1921 cgtcgaatac actgttgcca aagacgacga agcactcgaa gcctttgact tgctctgccg
     1981 cttcgagggc atcatccccg cgctcgaatc cagccacgcc gttgcttggg cggtgaaaaa
     2041 cgcgccgaaa atgggcaaag accaagtgat tctggtcaac ctatccggtc gtggcgacaa
     2101 agacatcaat accgtcgcga agctcaaagg gattaaactg taacctcgtc cgtct
//
LOCUS       DAWKST010000215         2148 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.215, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000215 DAWKST010000000
VERSION     DAWKST010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.215"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            27..572
                     /locus_tag="WJL87_001950"
     CDS             27..572
                     /locus_tag="WJL87_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ7800341.1"
                     /translation="MGKTVTLTSGHSNTDPGAVNGSDREADLAQDMCNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAARV"
     gene            569..775
                     /locus_tag="WJL87_001951"
     CDS             569..775
                     /locus_tag="WJL87_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800342.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAARKRKNRR"
     gene            778..942
                     /locus_tag="WJL87_001952"
     CDS             778..942
                     /locus_tag="WJL87_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800343.1"
                     /translation="MGQVAFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYQEML
                     KRHLQTKSVE"
     gene            977..1267
                     /locus_tag="WJL87_001953"
                     /pseudo
     CDS             977..1267
                     /locus_tag="WJL87_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1164..1469
                     /locus_tag="WJL87_001954"
     CDS             1164..1469
                     /locus_tag="WJL87_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800344.1"
                     /translation="MSLLKTVKMQATVVLTALAITACSSTSAPSATEIKVVEKAVMPT
                     PPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQNQAWRDWAGNHSRKVGN"
     gene            complement(1514..1684)
                     /locus_tag="WJL87_001955"
     CDS             complement(1514..1684)
                     /locus_tag="WJL87_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEZ7800345.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            1697..1915
                     /locus_tag="WJL87_001956"
     CDS             1697..1915
                     /locus_tag="WJL87_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800346.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            1912..>2148
                     /locus_tag="WJL87_001957"
     CDS             1912..>2148
                     /locus_tag="WJL87_001957"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800347.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFCVAFSATAPADVIGILRKYYS"
BASE COUNT          575 a          484 c          603 g          486 t
ORIGIN      
        1 caatcaatat tttttggaga tgatgaatgg gcaaaaccgt aaccttaacc tctggacaca
       61 gcaacaccga cccgggtgcg gtcaacggca gcgaccgtga ggcggacttg gcgcaggata
      121 tgtgcaatat cgtggctgct attttgcgcg atgactacgg tttgactgtt aaaaccgacg
      181 gcacgggcaa aggcaatatg ccgctgcgtg aagcggtcaa gctgattcgc ggctcggatg
      241 tggcgattga gtttcacacc aacgctgccg tcagcaaagc ggcgacaggc atcgaagcct
      301 tgagtaccgt taaaaacaaa cgctggtgtc aggtgttgag caaagccgtt gccaagaaaa
      361 ccggctggaa actgcgcggc gaagacggct ttaaacccga caatgcgggc cagcattcgc
      421 gcctggctta tgcacaagcc ggcggcattg tgtttgagcc ttttttcatc agcaacgaca
      481 ctgatttggc cttgtttaag acgactaaat ggggcatctg ccgcgcgatt gcggacgcga
      541 ttgcgatgga attgggggcg gcaagagtat gaatattatt ggtaaattga aagaagctgc
      601 ttcctatttt cttacaaaat tgattggaga aaatcctagt aatgagcagg taaaccgcgc
      661 acttatacag atgccaaatg ttcgtccgat acacacctat ccacgcccaa atttaagaaa
      721 ctcaggcgtg gcagccgcaa aacgggcggc gcgtaaacgc aagaatcgtc gttaatcatg
      781 ggacaggttg cgttttacga aaagatgatt gggctgtggt cggccaaaag ccgtgaggca
      841 agcgaacagg cagacttggc ggcgtttgaa tttgcggagg gcgaactggc caattatcag
      901 gaaatgctga aacggcacct gcaaaccaaa agtgtggaat agcaatgcgt attttggata
      961 tttttaaaaa cccagcgaca ggcaggaaaa agacaaagcg caagccctgc tgttgtcggc
     1021 tcaaaactac gcccgcgaac tggaacaggc gcgcgcggaa gcgaaaaaat atgaagtcaa
     1081 ggcgcacgcc gtcggcatgg ctttggcgaa aaaacaggcg gaagtcagcc gtctgaaaac
     1141 ggaaaataaa aaggaaatcg aaaatgtcct tactcaagac cgtaaaaatg caggcgacgg
     1201 ttgtattgac ggctttggcc atcacggctt gcagctctac aagcgcgccc tcggctacgg
     1261 aaattaaggt tgtcgaaaag gcggtcatgc cgacaccgcc tgccgcattg atggtcgcgc
     1321 cggtacgccc aaatccgccg aaagacggca agacggccac gctgttggaa cacgccgccg
     1381 agtttggcgg ctatgtggcg gagttggaaa atcaaaatca ggcttggcgc gactgggcgg
     1441 gcaatcactc ccgcaaagtc ggaaactgac aaaaaagccc gcgtagggcg cgggctgagg
     1501 gtgaaagcgg attttatacc tcttttacag gggtagcggc ggtagtgctt ttcagcaaat
     1561 cgactgcgtg ctgacagttt tgcttgctgg tgtagccttc gccctgagcg atgatttcat
     1621 ggttggctgc tttcaaacgc caacggtatt cgccttttgc gtctttatag atttcaaaat
     1681 acataaggtt tctcctatga atgagtacac gttttcttac cgctttaacg gcaagtcctg
     1741 gtcattgagc atttgggcgg acaaccctga agaagccagg gcgaaatttc gggctgcacg
     1801 agaaaatgcg cactatgacg gcgaagttgt agcaaaggtt tatacatttg taaatatttc
     1861 gtgggttaag aaattgtaca agcggacaaa atatttaatg ggtatcaaag aatgacctac
     1921 cgtgaattag ttgaacgtca gttggctgtg cgccatgccg atttggaatt gggcttaagc
     1981 cgcgcacgcg agcaagagcc gtttgtcatt catgtttccg atctgttgga taaggcaggc
     2041 attgagtacg cggtacgcat ggataaggat tttcagacga ccttttgtgt ggcgttttct
     2101 gcgaccgccc ctgctgatgt gattggtatt ttacggaaat attactca
//
LOCUS       DAWKST010000216         2108 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.216, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000216 DAWKST010000000
VERSION     DAWKST010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.216"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(42..365)
                     /locus_tag="WJL87_001958"
     CDS             complement(42..365)
                     /locus_tag="WJL87_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ7800348.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKARLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(353..592)
                     /locus_tag="WJL87_001959"
     CDS             complement(353..592)
                     /locus_tag="WJL87_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HEZ7800349.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(715..1512)
                     /gene="truA"
                     /locus_tag="WJL87_001960"
     CDS             complement(715..1512)
                     /gene="truA"
                     /locus_tag="WJL87_001960"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ7800350.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQI
                     AGEAVSTTVAGRTDTGVHATAQVVHFDTTAARPQQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRRAAAYLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSSGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
BASE COUNT          485 a          495 c          589 g          539 t
ORIGIN      
        1 ttaatccact ataatgaaca caatcaattc agactattca atcaggcaaa catctcctgc
       61 aatactgcaa acagtttttc agccgtactg ttgtctaaat ttccaagatg cttgaccaat
      121 ctggctttat ccacagccct aatctgttcg ggcaaaagca aaccgtcttt atcctgaaag
      181 cggacattga cgcggaacgg ggcaggacgg cttccgctcg tcataggaac gatcagcaca
      241 gtcttgagat agttgtgtat ttcaggagga gatactacga cacaaggacg tgtctttttg
      301 atttcgcttc ctacggtcgg gtctaaggag accagataga ttccgccgcg tactaccata
      361 tccattcttt atccgcttcg ttttcaattt cggaaaaaaa atgctcctgc tcggtttcga
      421 caagcattgc ggcagcttct gcccatcccc tgcgaacggt aggacagctt aaaatgatat
      481 tgcccttttc aactgtaaca gccaagctgt ctactgcccc tatttgaccc aataatgatt
      541 tgggcagaat cacgccttgc gagtttccca ttttgcgtat gttgagaatc atatacgtac
      601 catattcacc agtttatgta ataacaatgt tagtaccttg atgaggtagt gtcaacattg
      661 aaaaagattg cctgacagtt tgtccgattt caaaatctcc gcgacaagaa tgttttaaag
      721 ccattcgggg atttgggggc ggatgatgcc gtatgcctcg ggatagtcga cgccggtcag
      781 gtaaagcccg tcgggcataa aggtcggtgg ggctttgagg cggctgcgtt cttgaatcag
      841 tgcggcgaag ccttcgacgc tgagtctgcc gctgccgaca taaacgagcg cgcccatgat
      901 gttgcgtacc atgtggtgca aaaaggcgtt gccgtgcaaa tcgaggcgga cgagtcctga
      961 gctttgggta aggtcggcgc ggtagatggt tttgacgggg gattttgctt ggcattcggc
     1021 ggcgcggaag ctggagaagt cttgttcgcc gatcaaatag gcggcagccc gacgcatcgg
     1081 ttcgatgtcg agtttgaggt gtgtccagcc tgccctgttt ttgagcaggg gggaacggac
     1141 gggtgcggat tcgagcaggt agcggtagtg ccgtccgtat gcgtcaaatc gtgcatgaaa
     1201 ttcgggggcg acctgtcggg cgtgcaaaac ggcaatgcct tcgggcaggt gggcatttac
     1261 gccgcgcacc catgcctgtt ggggacgggc ggcagttgtg tcgaagtgga cgacttgggc
     1321 ggtggcatgc acgccggtgt cggtcctgcc ggcaacggtg gtggaaaccg cttcccctgc
     1381 tatttgggcg agcgcggttt ccaatgccgc ctgaacggtc ggtacgccgt cagcctgttt
     1441 ctgccagccg taaaagcggc tgccatcata ggatagggtt attgcccagc gttgtttttg
     1501 tgcggtatcc atcggatttg ggattcggat aaatgttcag actgcattgt atcgcagatt
     1561 ttgcagggaa acggcaaatg cccggggcgc gcggcgttgt ttggggagtt ggggggggga
     1621 tgcagttgct acgaatcgct atcctgtgaa ttcaccctgt caggagtgcc cgaatcgtca
     1681 ttcccgcgca gtcgggaatc taggacgtaa aatctaaaga aaccatttta tagtggatta
     1741 acaaaaacca gtacggcgtt gcctcgcctt agctcaaaga gaatgattct ctaaggtgct
     1801 gaagcacgag tgaatcggtt ccgtattatt tgtactgtct gcggcttcgt cgccttgtcc
     1861 tgatttttgt taatccacta tatccgataa gtttccgcac cgacaaaact agattcccac
     1921 tttcgtggga atgacgggat gcaggttcgt gggaatgacg cgaacagaaa cctcaaatcc
     1981 cgtcattccc gcgcaggcgg gaatctagac cttagaacaa cagcaatatt caaagattat
     2041 ctgaaagtct gggattctgg attcccactt tcgtgggaat gacggaatgc aggttcgtag
     2101 gcatgacg
//
LOCUS       DAWKST010000217         2056 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.217, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000217 DAWKST010000000
VERSION     DAWKST010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.217"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(83..1162)
                     /gene="aroB"
                     /locus_tag="WJL87_001961"
     CDS             complement(83..1162)
                     /gene="aroB"
                     /locus_tag="WJL87_001961"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ7800351.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIITNET
                     VAPLYLHTLQTALDAAGVSHFSIILPDGEAYKNWQTLNLIFDGLMQHRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFIQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPARELSAGMAEVIKYGTLGDISFFEWLEQHMPELMALERAPLIQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
     gene            complement(1242..1754)
                     /locus_tag="WJL87_001962"
     CDS             complement(1242..1754)
                     /locus_tag="WJL87_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ7800352.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPEILLERTRCDNSRPLLQVADPLEKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
BASE COUNT          404 a          493 c          657 g          502 t
ORIGIN      
        1 ggattcccgc ttttacggag aaagatgatg gaaaatcatc atcgtgcgtc aaacccgcgc
       61 ggcagcacat cggcagagga aatcagagat acggttgcag ggtgcggcgg aggatgtccg
      121 tgtcggtaat ttcggtgatg ttggcttcgc ccagccggtt cagaccgata aagcgcatga
      181 tgccgccgct gactttttta tcgtggctca tatgttcgag ccatttttca aaggcaaaca
      241 cgggcggtgc ggacggcagt ccggcggctt cgagcagggc ggcgagccgc gcggtatctg
      301 cggcggaggt tttgcccagt tgttcggaca aacgcgccgc caacacgcag ccggcggcga
      361 tggcttctcc atgcagccaa gtgccgtaac ccatctcggt ttcaatggcg tgtccgaagg
      421 tgtgtccgag gttgagccat gcgcgtatgc cctgttcggt ttcgtcttgg gcgacgatgt
      481 ctgccttcat ttggcaacag cggtacacgg cttggatgag gggcgcgcgt tcgagtgcca
      541 tcagttcggg catatgctgt tccagccatt caaaaaaact aatgtcgccg agcgtgccgt
      601 atttgatgac ttccgccata ccggcggaca gttcgcgggc gggcagggtg tgcagcgtgt
      661 ccaagtcggc aagcaccgcc tgcggctggt agaacgcgcc gatcatattt ttgccgagcg
      721 ggtggttgat ggcggttttg ccgccgacgg aggaatcgac ctgactcaac agtgtggtcg
      781 gtatttggat gaacggagcc ccacgctggt aggtggcagc ggcaaagccg accatgtcgc
      841 cgataacgcc gccgcccagc gcgattaatg tggttttgcg ttcggcgcgg tgttgcatca
      901 gaccgtcaaa gatgaggttg agcgtctgcc agtttttgta cgcctcgccg tcgggcagga
      961 tgatgctgaa atgggacacg cctgccgcgt ccaatgccgt ctgaagcgtg tggagataaa
     1021 gcggggcgac ggtttcgtta gtgatgatgg cggcgcgttt gcccaaatgc ggtttgagca
     1081 ggctgcctgc ctgcggcagc agcccgttgc cgataaagat ggggtagctg tgggacgggg
     1141 tgtgtacggt cagtgttttc attgttgttc cttaaagttt gaaccgccgg cccgccgggc
     1201 ggggcgcggt ttggtttttt gggcggggcg catatgccgg tttatcggga taagcgctta
     1261 agcagggttt gcacggtttc ccggcagttt gccgattcta cggtaaagtc ggcagtttgg
     1321 cggtaaacgg ggtcgcgtgc ggcgtagagt tcgcgcaatt tctccaaagg atcggcaact
     1381 tgtaaaaggg ggcggctgtt gtcgcagcgc gtgcgttcga gcaggatttc gggcggggcg
     1441 tgcagataga cgaccgtgcc gcttttgcgg ataagggcgc ggttttcttc ttttaacacc
     1501 gcgccgccgc cggtggacag gacgatatgg ggcaggataa ccagcttttt gagtatggcg
     1561 gtttcgcgcg aacggaatcc ctgttcgcct tccatttcaa atatggtggg gatgggaacg
     1621 ccggcggctg cggcgatttc gtgatcgctg tcgtaaaaac ggtaatccag ccgctgcgcc
     1681 atttgccggc ccagcgtggt tttgcccgcg cccatcagtc cgatgaggat gagtttgccg
     1741 ttaaagtttt tcatcacaat tccttaatgt ttgacacccc gcctttcggg gcgacagggt
     1801 tcgggcttgt cgggttacgg cggaatttta tacgaaatcg gcagggcggc ggtacgtttg
     1861 gaaaaataac ccgaccatcc cgaacctttc tgattttaag gaaaaataaa agaaatcagg
     1921 gaggtttttt atttcaggct gtgttttgac aatccgttga tttcacttat ttgtcaggaa
     1981 aaggcaatta tctttgctta ggtaaacaat tatccaattg aatatattga agataatatg
     2041 tttatcaata ctatag
//
LOCUS       DAWKST010000218         2038 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.218, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000218 DAWKST010000000
VERSION     DAWKST010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.218"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            305..1762
                     /locus_tag="WJL87_001963"
     CDS             305..1762
                     /locus_tag="WJL87_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7800353.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGITLIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFIPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKCYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          470 a          599 c          464 g          505 t
ORIGIN      
        1 ctataatgcg cccttcggcg tggcggatat ataaggaagt gattttccat ctaagtaaaa
       61 accgccctat cggataagcc cttaacagaa aaggctttac ccgcgccgta tcggaacaca
      121 tcctctaaaa tacaatccgt tgaattgaaa aaaatataaa aacatccgcc cgcgaaaaac
      181 ggcagcgcgt cgtttgacaa agaatgaaaa tatcggttaa aaaccgattt tcatacaaaa
      241 aacaccgctg ccgtccgcat ccgtttcaga cggtattgag agaaaatctt ttaggagaac
      301 ctttatgtcc cggcatcccg cccccaccgg agaaaaaaca ttcttcggcc accccttcca
      361 gctttccacc ctcttccata tcgaattgtg ggaacgtttt tcattttacg gaatgcaggg
      421 catcctgctg atttacctct actacaccgc cgacaaaggc ggcttgggca tagacaaaac
      481 cctcgccggc ggcattgtcg gcgcatacag cggcagcgtg tacctgtcca ccattttggg
      541 ggcgtggttt gccgaccgag tatggggtgc ggaaaaaacc ctcttcctct cgggcatcgt
      601 cgtgatgctc ggacacatcg tccttgccgc cgccccgggc ctgtacggtc ttttaatcgg
      661 gctgatattc atcgcattgg gcagcggcgg tgtgaaatct acggccagtt ctatggtggg
      721 cgcattatac gaacaggacg aaatgcgccc gctgcgcgat gcgggatttt ccattttcta
      781 catcgccatc aacatcggcg gcttcctggg cccgctgctg accggcctgc tgcaggaaaa
      841 catcggtttc cattatggtt tcggcgcggc ggcggtcggt atggcattcg gcttgtggcg
      901 ttattccttg ggacgtaaaa acctgcccca ccccaccgtc ccccatccgc tttcaaaagg
      961 acagggcaaa actgcggccg ccgtcggcat caccctcatc gccgcacttg caaccgccat
     1021 caaaaccggg cttgtcaacc tcgacaattt ctccggcatc ctcttatcta ccgtcatcct
     1081 tgccgtcatt gcctatttcg cccgcctgct gaccaacccc cgcgtcagtt ccgacaacaa
     1141 acggcacatc atcgcctaca tcccgctttt cctgaccatc tgtatgtttt gggccgtttg
     1201 gtttcagatt tacaccgtag caaccgtcta tttcgacgaa accgtcaacc gcaccatcgg
     1261 ttcgtttacc gtgcccgtcg cctggaaaga ttctatgcaa agcctgtggg tcatcctgtt
     1321 ttccggactt atggcggcaa tgtggacaaa aatggggcgc aaacagccca aaaccccgct
     1381 gaaattcgct atggcggtat ttgttaccgg cgcgtcgttt ttgggattca tcccctttat
     1441 ttccgccggc acgccgatgc ctattgcggt tttcgcactg attgtccttg ccatcacgat
     1501 aggcgaactg atgatttccc cgattgcgct gtccatctcc accaaaatcg caccgccttt
     1561 attcaaaacc caaatggtcg cccttaattt ccttgccttt tcattaggct tcactttggg
     1621 cggcgtattg tttgaaaaat gctatcaggc gggcgacgaa atcggcttct atcggctgct
     1681 gttctacatc ggcgcagcca caggcttcct gctgctcctg ctcgtcccca aattgaacaa
     1741 aatgctcgaa ggcacagact aagtcccgcc ccgatgccgt ctgaaccctt cagacggcat
     1801 ttttccgcat aatgaaacca aaccgtttcc acccgacagg acaggctccc cccaaccgga
     1861 aggcagcctg ccgattgtca tttaaatatc tgactgattc cgtctgtttg caagggaaat
     1921 agccgtttat ttccgcttgg gcggaaaccg gttctattga gtaaaaggca ttttatccga
     1981 ctaaattagt gctgcatcaa cggaatatat agtggattaa caaaaatcag gacaaggc
//
LOCUS       DAWKST010000219         1995 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.219, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000219 DAWKST010000000
VERSION     DAWKST010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.219"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..96)
                     /locus_tag="WJL87_001964"
     CDS             complement(<1..96)
                     /locus_tag="WJL87_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ7800354.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVG"
     gene            357..1829
                     /gene="pyk"
                     /locus_tag="WJL87_001965"
     CDS             357..1829
                     /gene="pyk"
                     /locus_tag="WJL87_001965"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ7800355.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     LLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQVEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
BASE COUNT          520 a          567 c          514 g          394 t
ORIGIN      
        1 gcctactaac tctgctgctg ttcttgcagt tacacctgcg acaaacagtt caatgagttt
       61 attttgttta taccggctta gacgactttt tctcataggg ataattctaa cttaatttga
      121 atttccctag ttatctagga cagtcccaaa aataaattta gcctaagtca taaaaccgtg
      181 taattttgca ccaaaaaagg cggtttgcag gtaaaatatc aaactattaa tttaaatcat
      241 atttttacag aatattccgc cgcgttcatc aaatggacat caaaccggtt ccaaatttct
      301 gtaattttgt aacaaaatac cgcaaaacac ccgattgaga ccaaaaggac tttcatatga
      361 accagacaag ccgcgatctg acccgcatca gccacaacac caaaatcgtc gccacccttg
      421 ggccgggcag caacaacgtc gaactgttgg aagatatgat ccgcgtcggc ggcctgaacg
      481 tcgtccgctt caacttcagc cacggcacgc ccgaattcca tcaggaaaac gcccgcatcg
      541 tgcgcgaagc ggcaaaacgc gccggacagg aaatcgccat cattgccgac ctgcaagggc
      601 cgaaaatccg cgtgggcaaa atcgccggcg gcggcatcga attgaacaaa ggcgaaacgc
      661 tgctactgga tgccgcgctt gaaggcgaag gcacgcgcga ggcggtcggt ttggactacc
      721 gcgacctgcc cgatgacgtt gccgcaggcg atgtcttgtg gctggacgac ggcctgctga
      781 ccctgaccgt ggaatccgtc gaaggcagca ggattatcac aagggtggaa aacagccacg
      841 tcctgaaaag caacaagggc atcaacaaac gcggcggcgg tctgtccgca ggcgcgttga
      901 ccgaaaaaga cttccgcgac ctgaaaaccg cgattgccat cggttgcgac tacctcgcca
      961 tcagctttgt gaaatccgcc gaagatttgc atatcgcgcg cgccaaagtc gaagaagaaa
     1021 tgaaaggcag cacggccgtg cgccccggtt tggtttccaa aatcgaacgc gtagaagcga
     1081 ttgaaaactt ggacgaaatc atcctcgccg gcgacggcat tatggttgcg cgcggcgact
     1141 tggcggtcga agtcggacac gccgccgtcc ccgccctgca aaaacggatg atccgccgcg
     1201 cccgcgagtt gcgccgcttc agcattacgg cgacgcaaat gatggaatcg atgattacca
     1261 accccgtacc gacccgcgcg gaagttagcg atgtggcaaa cgcggtattg gacggtaccg
     1321 atgcggtgat gtgttccgcc gaaaccgccg tcggcgcgta tccgtttgaa accgtcagcc
     1381 aaatggcgat tatctgcgcg gctgcggaaa aagagcagga ttcgctcaac ggcgttgccg
     1441 aacaggtcga gtatcccgaa gcggtcagca ccaacctggc ggttgccggc ggtgcggtca
     1501 gcgtggcgcg cgcggttcac gccaaagcca tcgtcgccct gaccgaaagc ggttcgaccg
     1561 cctttgaaat cagccgccac aacatcaccc tgccgatttt cgcgctgacc ccgagcgttt
     1621 ccgcccaacg ccgtatggcg atgtaccggg gcgtgcgccc gctgattttg gcaaccagca
     1681 ccgaccacga cacggcgtta aacgaagtcg aaaccatgct ggtggaacac aacatcctgc
     1741 attccggcga ccaatacatc attaccagcg gctcgcaaat gcgcgaatcc ggttcgacca
     1801 acacactgga agtgctgcgc gtcaaataat ccgccctgag tggaaaatgc cgtctgaagt
     1861 cgatgcccga ggcttcagac ggcattttat ttgcattccg tcaggtaaaa ccgaaaaaat
     1921 tgtgcaaatc cggcaacatc agataaaatt gagtacctat actaaagcga aacaaggcat
     1981 ttccgactgc ctttt
//
LOCUS       DAWKST010000220         1979 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.220, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000220 DAWKST010000000
VERSION     DAWKST010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.220"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..92)
                     /locus_tag="WJL87_001966"
     CDS             complement(<1..92)
                     /locus_tag="WJL87_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin family protein"
                     /protein_id="HEZ7800356.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMANDEMFKP"
     gene            complement(93..377)
                     /locus_tag="WJL87_001967"
     CDS             complement(93..377)
                     /locus_tag="WJL87_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HEZ7800357.1"
                     /translation="MKLLAALIPLLMSVAGRILTALGLMAVTYSGVDRLVAHFQQAIT
                     SSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            complement(834..>1979)
                     /locus_tag="WJL87_001968"
     CDS             complement(834..>1979)
                     /locus_tag="WJL87_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ7800358.1"
                     /translation="PETDKFVKGYEYSNCLWYEDERRINRTYGCYGVDSSIMRLMYDD
                     SRFPEVKELMESQMERLARPYWEKLRHRPDMSYFKNYNFKRCYFGLNGGDCLVAKGDD
                     GRTFISFSLQGNSKYKEEMDAKKLEEILSLKVDANPDKYIKATGYPGYSEKVEVAPGT
                     KVNMGPVTDRNGNPVQVVATFGRDSQGNTTVDVQVIPRPDLTPGSAEAPNAQPLPEVS
                     PAENPANNPDPNENPGTRPNPEPDPDLNPDANPDTDGQPGTSPDSPAVPDRPNGRDGK
                     DGGLLCKFFPDILACDRLPESNPAEDLNLPSETVNVEFKKSGIFQDSAQCPAPVTFTI
                     TVLDSSKQFAFSFENACTIAERLRYMLLALAWAVAAFFCIRTVSREV"
BASE COUNT          447 a          505 c          490 g          537 t
ORIGIN      
        1 ggcttaaaca tttcatcatt cgccatcatg gaaaccattt ttaatgtttt ccctgaaccg
       61 ggcgtgccgg ttatcaaaca gatctctgcc atttattttt tcttcccgat tgaggttgct
      121 agttttgtca tttgtttgaa tgacagaata aaggcgatcg cgccaaacag gatattaaga
      181 acggtgccgc caccgcttat ataaaagagc tgcaacatcg cttgaggcgc gcccgttatg
      241 ctactggtta tcgcctgctg aaaatgggct accaatctat ctacacccga ataagtaaca
      301 gccatcaagc ctaatgcagt caatatacgg cctgccacgc tcatcaaaag cggaatcaat
      361 gcggccaaca atttcatttg ctatcccttt cttaaaaggc acggttgcct cattaaacaa
      421 atgtttctta atctgaaaga ttttgcgccg cattcgcggc gcggcggcgc tgtggggaca
      481 cccccctcgc gcttatcgcc aatccccccg cgcttctcgg ctatttgcga cattcgtcgc
      541 aaagtgcgct acttcgcctg cctttcggcc aaagtttccg aagctgaacg ctttgcgggg
      601 gactagtccc ccacaccccc tagtctcact tgcgacgccg cggaggcagg gggaacggcg
      661 caaaaggcgc gcgccttacc acctgccctt gcggcagagt gtgttctttt ggcggggcgg
      721 caaggggtat ccaaaaagat ttataaagac gataaagccg tctttacaaa tctttctgga
      781 cgtcctcccc ctgccttggt acaagttact gaagcccggc ggtgctgcgc ctgctagact
      841 tcacgagata ctgtgcggat acaaaaaaag gcggcaaccg cccaagcaag ggcgagaagc
      901 atgtacctta gccgttcggc tatggtacat gcgttctcaa agctgaacgc gaactgcttg
      961 cttgaatcaa gcacggttat agtgaacgta acgggggcgg gacactgtgc ggaatcttga
     1021 aagattccgg atttcttaaa ctctacattg acggtttcag acggcagatt taaatcttct
     1081 gccggattgg actcgggcag cctgtcgcaa gctagaatgt cggggaagaa tttgcacaaa
     1141 agcccgccat ctttgccgtc cctgccgttt gggcggtccg gaacggccgg ggaatcgggg
     1201 cttgttccgg gctgtccgtc cgtatcggga tttgcatcgg gattcaaatc ggggtcgggt
     1261 tcgggattgg gacgcgtgcc ggggttctca ttggggtccg ggttgtttgc ggggttttca
     1321 gcgggcgata cttcgggcag cggctgtgcg ttcggcgctt ccgcgcttcc gggtgtgagg
     1381 tcgggacgcg gaattacttg aacatccact gtggtgttgc cttgcgaatc cctgccgaat
     1441 gttgcgacaa cctgaacggg attcccgttc ctgtccgtga cgggtcccat attcactttt
     1501 gttccgggtg cgacttctac tttttcggaa taaccgggat atccggttgc ctttatgtat
     1561 ttgtcgggat tggcatcgac tttcaacgat aaaatctctt ccagcttttt ggcatccatt
     1621 tcttctttgt attttgaatt tccttgaagt gagaaactga taaaagttct accatcatca
     1681 cctttagcaa ctaaacaatc tccgccgttc aatccgaaat aacaacgttt aaaattgtag
     1741 tttttaaaat aagacatatc aggacgatgc cttaactttt cccaatacgg acgggcaagc
     1801 ctttccattt ggctttccat caattctttg acttcgggga atctgctgtc atcgtacata
     1861 aggcgcataa tagaactgtc aacgccgtag cagccatagg ttctattaat acgtctttcg
     1921 tcttcgtacc aaaggcaatt actatattcg taacctttta caaatttgtc ggtttcggg
//
LOCUS       DAWKST010000221         1951 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.221, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000221 DAWKST010000000
VERSION     DAWKST010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.221"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(498..1580)
                     /locus_tag="WJL87_001969"
     CDS             complement(498..1580)
                     /locus_tag="WJL87_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ7800359.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVEVSRVKDAGTYKA
                     QGGKSKTATQIADFGSKIGFKGQEDLGNGMKAIWQLEQKASIAGTNSGWGNRQSFIGL
                     KGGFGTVRAGNLNTVLKDSGDNVNAWESGSNTEDVLGLGTIGRVESREISVRYDSPVF
                     AGFSGSVQYVPRDNANDVDKYKHTKSSRESYHAGLKYENAGFFGQYAGSFAKYADLNT
                     DAERVAVNTANAHPVKDYQVHRVVAGYDANDLYVSVAGQYEAAKNNEVGSTKGKKHEQ
                     TQVAATAAYRFGNVTPRVSYAHGFKAKVNGVKDANYQYDQVIVGADYDFSKRTSALVS
                     AGWLKQGKGAGKVEQTASMVGLRHKF"
BASE COUNT          464 a          431 c          498 g          558 t
ORIGIN      
        1 ttagcttgtg ggtatttacc ggaaaaaaca gaaaccgctc cgccgtcatt cccgcgcagg
       61 cgggaatcta ggtctgtcgg cacggaaact tatcaggtaa aacggtttct tgagattttg
      121 cgtcctggat tcccactttc gtgggaatga cgggatgcag gttcgtggga atgacggaat
      181 gtaggttcat gggaatgacg cggtgcaggt ttccgtacgg atgaatttgt cattcccgcg
      241 caggcgggaa tctaggtctg tcggtgcgga aacttatcgg gtaaaacggt ttcttgagat
      301 tttgcgttct agattcccac tttcgtggga atgacggaat gtaggttcgt gggaatgacg
      361 gcggagaagt ttctgctttc ccgataaatg cccactctcg tgggaatgaa ggctgccgca
      421 ttaaatagca aacgaatcat ttcaaataaa aaagcctgtc tgacgacagg ctttttgttg
      481 ataccaatct tttcagatta gaatttgtga cgcagaccaa ccatgctggc agtttgttcg
      541 acttttcccg cgcctttacc ttgtttcaac caaccggcag aaaccagagc ggaagtgcgt
      601 ttggagaagt cgtagtcggc accaacgata acttggtcgt attggtaatt tgcgtctttc
      661 acgccattca ctttagcttt gaagccgtgg gcgtaagaaa cgcgaggcgt tacgttgcca
      721 aaacggtaag cggcagtagc ggcaacttga gtttgctcgt gttttttacc cttggtagaa
      781 ccaacctcgt tgtttttagc agcttcatac tgaccggcaa cagaaacgta caggtcattg
      841 gcatcgtaac cggcaactac gcggtgtact tggtaatcct taacaggatg ggcatttgca
      901 gtatttactg caacacgttc tgcatcagtg ttcaaatcag catatttggc aaaagaacct
      961 gcgtattgac cgaagaaacc ggcattttcg tatttcagac cggcgtggta agactcacgg
     1021 ctggacttcg tatgtttgta tttatccaca tcattcgcat tatcgcgcgg aacgtattgt
     1081 acgctgccgc tgaagcctgc aaatacggga gagtcgtagc gtacggagat ttcacggctt
     1141 tctacacgac cgatagtacc cagtcccagt acatcttcgg tgttagaacc agattcccat
     1201 gcattgacgt tgtcgccgct gtctttcaat acggtgttca gattaccggc gcggacggta
     1261 ccgaagccgc ctttcaagcc gatgaaggac tggcggttac cccagccgct gttagtgccg
     1321 gcgatggagg ctttttgttc caactgccaa atggctttca tgccgttgcc gaggtcttct
     1381 tgacctttga aaccgatttt agaaccgaag tcggcaattt gggttgcagt tttagatttt
     1441 ccgccttgag ctttatatgt accagcatct tttacgcgag aaacttctac gcctgcttta
     1501 attgtaccgt acaaggtaac gtcagccatt gctgcaacag gcagggcagc cagtgtcagg
     1561 gcaatcaggg attttttcat tgctgtattc cttttcatag ttaagaaatt taagccagcc
     1621 gggctttcca agccgcttag ctttgcattt accgccgacg ttttcggcga ttccatgtct
     1681 gaaactatag atgttcctgt ctgaaaaaac aaacttctgc ctgtttttgt gtggaattcg
     1741 atgtgtattt tgaagggcgg atagagatat tgtgggtatt ttttgtttta taacatatgg
     1801 ttatttaaat tttttaagat ttgcattttt acaacactta ctcgggaggg tattggaggg
     1861 cattgcaaac cgggggttat aaagacggca aaaaacgcgc acgtggtttc tttcggaacg
     1921 ggcggatgca aaacccgacc ctgcggcagg a
//
LOCUS       DAWKST010000222         1863 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.222, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000222 DAWKST010000000
VERSION     DAWKST010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.222"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..92)
                     /locus_tag="WJL87_001970"
     CDS             complement(<1..92)
                     /locus_tag="WJL87_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin family protein"
                     /protein_id="HEZ7800360.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMANDEMFKP"
     gene            complement(93..377)
                     /locus_tag="WJL87_001971"
     CDS             complement(93..377)
                     /locus_tag="WJL87_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HEZ7800361.1"
                     /translation="MKLLAALIPLLMSVVGRILTALGLMAVTYSGVDRLVAHFQQAIT
                     HSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQITKLATSIGKKK"
     gene            complement(680..>1863)
                     /locus_tag="WJL87_001972"
     CDS             complement(680..>1863)
                     /locus_tag="WJL87_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ7800362.1"
                     /translation="IQARGYQYDPETDKFVKGYEYSNCLWYEDERRINRTYGCYGVDS
                     SIMRLMSDYSRFPEVKELMESQMYRLARPFWNWHKEELNKLSSLDWNNFVLNRCTFDW
                     NGGDCVVNKGDDYRNGANFSLIRNSKYKEEMDAKKLEEILSLKVDANPDKYIKATGYP
                     GYSEKVEVAPGTKVNMGPVTDRNGNPVQVVATFGRDSQGNTTVDVQVIPRPDLTPGSA
                     EAPNAQPLPEVSPAENPANNPAPNENPGTRPNPEPDPDLNPDANPDTDGQPGTSPDSP
                     AVPDRPNGRDGKDGGLLCKFFPDILACDRLPEPNPAEDLNLPSETVNVEFKKSGIFQD
                     SAQCPAPVTFTITVLDSSKQFAFSFENACTIAERLRYMLLALAWAVAAFFCIRTVSRE
                     V"
BASE COUNT          429 a          454 c          460 g          520 t
ORIGIN      
        1 ggcttaaaca tttcatcgtt tgccatcatg gaaaccattt ttaatgtttt ccctgaaccg
       61 ggcgtgccgg ttatcaaaca gatctctgcc atttattttt tcttcccgat tgaggttgct
      121 agttttgtta tttgtttgaa tgacagaata aaggcgatcg cgccgaaaag aatgtttaga
      181 acagtaccac cgccgcttat atagaaaagc tgtaacattg cttgaggtgc gcccgttatg
      241 ctatgggtta tcgcctgttg aaaatgggct accaatctat ccacccccga ataggtcacg
      301 gccatcaatc ccaatgcagt caatatacgg cctacgacgc tcatcaagag cggaatcaat
      361 gcggccaaca atttcatttg ctatcccttt cttaaaaggc acggttgcct cattaaaaaa
      421 atgtttctta atctgaaaga ttttgcgggg actagccccc acacccccag tctcacttgc
      481 gacgctgcgg gggcaggggg acggcgcaaa aagcgcgcgc cttaccacct gcccttgcag
      541 cagagtgtgt tcttttggcg gggcggcaag gggtatccaa aaagatttat aaagacgata
      601 aagccgtctt tacaaatctt tctggacgtc ctccccctgc cttggcacaa gttactgaag
      661 cccggcggtg ctgcgcctgc tagacttcac gggatactgt gcggatacaa aaaaaggcgg
      721 caaccgccca agcaagggcg agaagcatgt accttagccg ttcggctatg gtacatgcgt
      781 tctcaaagct gaacgcgaac tgcttgcttg aatcaagcac ggttatagtg aacgtaacgg
      841 gggcgggaca ctgtgcggaa tcttgaaaga ttccggattt cttaaactct acattgacgg
      901 tttcagacgg cagatttaaa tcttctgccg gattgggctc gggcagcctg tcgcaagcta
      961 gaatgtcggg gaagaatttg cacaaaagcc cgccatcttt gccgtccctg ccgtttgggc
     1021 ggtccggaac ggccggggaa tcggggcttg ttccgggctg tccgtccgta tcgggatttg
     1081 catcgggatt caaatcgggg tcgggttcgg gattgggacg cgtgccgggg ttctcattgg
     1141 gggccgggtt gtttgcgggg ttttcagcgg gcgatacttc gggcagcggc tgtgcgttcg
     1201 gcgcttccgc gcttccgggg gttaagtcgg gacgcggaat tacttgaaca tccactgtgg
     1261 tgttgccttg cgaatccctg ccgaatgttg cgacaacctg aacgggattc ccgttcctgt
     1321 ccgtgacggg tcccatattc acttttgttc cgggtgcgac ttctactttt tcggaataac
     1381 cgggatatcc ggttgccttt atgtatttgt cgggattggc atcgactttc aacgataaaa
     1441 tctcttccag ctttttggca tccatttctt ctttgtattt tgaattgcga ataagggaaa
     1501 aattagcccc atttctgtaa tcatcacctt tattgaccac acaatctccg ccgttccaat
     1561 caaatgtgca acgatttaaa acaaaattat tccaatccaa agaacttaat ttattcagtt
     1621 cttctttatg ccaattccaa aacggacgtg ccagcctata catttggctt tccatcaatt
     1681 ctttgacttc ggggaatctg ctgtaatcgg acataaggcg cataatcgaa ctgtcaacgc
     1741 cgtagcagcc ataggttcta ttaatacgtc tttcgtcttc ataccaaagg caattactat
     1801 attcgtagcc ttttacaaat ttgtcggttt cggggtcgta ttggtagcct cgtgcctgta
     1861 tgt
//
LOCUS       DAWKST010000223         1851 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.223, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000223 DAWKST010000000
VERSION     DAWKST010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.223"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..107)
                     /locus_tag="WJL87_001973"
     CDS             complement(<1..107)
                     /locus_tag="WJL87_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ7800363.1"
                     /translation="MSTFFRQTAQAMIAKHIDCFPLLKLDQVIDWQPIE"
     gene            148..576
                     /locus_tag="WJL87_001974"
     CDS             148..576
                     /locus_tag="WJL87_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ7800364.1"
                     /translation="MGNFAKVSGYTYNKSRYRNAAEVNAERLAKNSSADPYNFSNFTP
                     VHIFRFGTSFHIPNTGLTVGGGVSAQSGTSSLYNIRQGGYGLIDGFVRYELGKHAKLS
                     LIGTNLNGRTYFENNYNRTRGANNFYGEPRTVSMKLDWQF"
     gene            720..1778
                     /gene="dinB"
                     /locus_tag="WJL87_001975"
     CDS             720..1778
                     /gene="dinB"
                     /locus_tag="WJL87_001975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ7800365.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLIARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
BASE COUNT          439 a          496 c          508 g          408 t
ORIGIN      
        1 tgttcgattg gttgccaatc aatcacctgg tccaacttca atagtgggaa gcagtcgatg
       61 tgcttggcaa tcatggcttg ggcggtttgc cggaagaagg tgctcatgag aaatccccta
      121 aatgtcttgg tgggaattta ggggattttg gggaattttg caaaggtctc agggtatacc
      181 tacaataaga gcaggtatag gaatgcagcc gaagtcaacg ccgaacgcct tgccaaaaat
      241 tccagtgcag acccgtacaa cttcagcaat ttcacacccg tgcacatatt ccgtttcgga
      301 acgagcttcc atataccgaa tacggggctg accgtcggcg gcggcgtgtc cgcacaaagc
      361 ggcacaagca gcctgtacaa catccgtcag ggcggctacg ggctgataga cggtttcgtc
      421 cgttacgaat tgggcaaaca cgccaaattg agcctcatcg gtacgaactt aaacggacgc
      481 acttattttg agaacaacta caaccgtacg cgcggcgcaa acaacttcta tggagagccg
      541 cgcactgtca gcatgaaact ggattggcag ttttaattga aaaccgtagt tttgcggatg
      601 ccgtctgaaa cagggtatgt ttcagacggc atttttatgg aggcaggcct atcggggcgt
      661 atatttggaa tttgcccgat gccggcagta gaatatccct tttatcctca gaagagccga
      721 tgtcttcacg caaaattatc cacatcgaca tggacgcatt ctacgcatcg gtagagctgc
      781 gcgaacagcc gcatttgaaa gggcggccgg tggtcgtcgc gtgggagggc gcgcgttcgg
      841 tgatttgcgc cgcatcgtat gaggcacggc agttcgggct gcattccgcg atgtcggtgg
      901 caacggcgaa aaggctgtgt ccgcaagcgg tgtatgtgcc gccgcatttc gatttgtacc
      961 gtcaggtttc cgcgcagatt cacgccgtat tcaggcgtta taccgattta atcgaaccct
     1021 tgtcgctgga cgaagcctat cttgacgtta cccgtaattt caaaaacatc ccttacgcca
     1081 gcgaagttgc caaagaaatc cgtgccgcca tttttgcgga aacaggtttg actgcatccg
     1141 caggcatcgc gccgaacaaa tttctggcga aaatcgcgtc ggactggcgc aagccgaacg
     1201 ggcagtttgt gttgccgccg cacaaagtca tggcattttt ggaaaccctg cctttgggca
     1261 aaatccccgg cgtgggcaag gtaacgctga aaaaaatgca gtcgctgggt atgcggacgg
     1321 cgggcgactt gcgccgtttc gagcgcggcg aactcttaaa ccatttcgga cgctacggat
     1381 accgcctcta tgatttggtg cgcggtacgg atgaacgccc cgtcaaagcc gaacgcgaac
     1441 gcctccaaat ctccacagaa atcaccctgc ctgaagacct gccgctcgag caggctgccg
     1501 gacacctgcc ccatcttgct gaagacctgt ggcggcaaat cacgcgcaaa aacgtcgaag
     1561 cccaaagcgt aacgctcaag ctgaagacct acgatttccg catcatcacg cgcacactga
     1621 cttattcctc cgtattgccc gactgcgcag ctctgctgca ggctgcgcaa atgttgatag
     1681 cgcgcgtccc gccgcagacg gaagacgcgt tccgccttat cggtatcggc gtggggcatc
     1741 ttgtgccgaa aaaccagcag caggatttgt gggcgtaaac cgctttacgc gcgccgtcca
     1801 aaaaacatgc cgtctgaacg gttttcagac ggcatggctg ctttatagtg g
//
LOCUS       DAWKST010000224         1827 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.224, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000224 DAWKST010000000
VERSION     DAWKST010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.224"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            50..1219
                     /gene="metZ"
                     /locus_tag="WJL87_001976"
     CDS             50..1219
                     /gene="metZ"
                     /locus_tag="WJL87_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ7800366.1"
                     /translation="MSKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VVSSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVANLEALAELAHGIGAILVVDNSLLSPIGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQAAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAALIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            1305..1511
                     /locus_tag="WJL87_001977"
     CDS             1305..1511
                     /locus_tag="WJL87_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ7800367.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
BASE COUNT          437 a          488 c          474 g          428 t
ORIGIN      
        1 caacgggcgc acgctccgtt gccgaacgga taaaaccgag gaattaccga tgagcaaaaa
       61 actccatccg caaacgctcg ccatacgcgg cggcaaagaa caaaccgaat accgcgaaca
      121 caatcaggcc ttgttcctga ccagcagctt catgtgggac aacgcccaac acgccgccga
      181 tttgttttca aaaaaaatca aaggcttcac ttatacccgt accgccaacc cgaccacagc
      241 cgcctttgaa aaacgcatcg ccgctttgga aggtgcggaa cgcgcggttg ccacttcgac
      301 cggcatgtcc gcgattcagg cggcgttttt cacctttttg caggcgggcg accatgtggt
      361 ttccagccgc agcctgttcg gcacgaccgt cggctttatc aataacatcg ttaccaaatt
      421 cggcatcgaa gtgagccacg tgtcgccgac tgatataaac gaatggaaag ccgccgtcaa
      481 agccaatacc aaactgctgt ttttggaaac gccgtccaac cccttgggcg aagtggccaa
      541 cttggaggca ttggcggaat tggcgcacgg catcggcgcg attttggtgg tggacaacag
      601 cctgctgtcg cccatcggct ctcagccttt gaagcacggc gcagatattt ccgtttcttc
      661 cgcaaccaaa gccatagacg gacacggacg cgtgatgggc ggcgtgttgg cgggttcgga
      721 agaactgatg gcgcaggccg ccgtttactg caactcttgc gggctggcga tgtcgccgtt
      781 taacgcgtgg cagttgttga gcggcgtgga aaccctgtcg ctgcgtatgg aaaaacagtt
      841 cgacaacgcc ctgaaaatcg cacaatggct gcaagcgcag ccgcaggttc aagccgtgta
      901 ctacaccgga ttgcccgacc atccgcaggc ggcgcttatc cgcaaacagc aaaacggcgg
      961 cggcatcgtc atcggctttg aagtcgccga ccaagcagcc gcgtggaaag tcgtggacgg
     1021 cgtggaactc ttttcccgta ccgccaacct cggcgacgtg cgctcgacca tcacccatcc
     1081 gtggacaacc acgcacggca ggatgcagcc cgaagaaaaa ctcgccgcca acatccgccc
     1141 cggattggtg cgcctgtcgg tcggtttgga atatgttggc gatttgattg acgatttgaa
     1201 acaggcactc gcacgctgat tgcgtgtttc agacggcatt ggacagcaga tgccgtctga
     1261 aacccttatc gcgtatggca tacacatcaa caataggaaa aaatatggat gaagcggttt
     1321 ttgccgattg ggcgttgaaa atctgcctga ccggtttgat tatttttttg ggttttatcg
     1381 tttggaattt gggcaaggag tccaaagcgg gcaaattcgg catcgccatt ttgtttttgg
     1441 ttttgggctt gggcgtgttc ggttttgtat ttaaagaact gctaatcaag tttttggtat
     1501 tgcccaaata ggctgccgcc tgtcaaaatg ctgtctgaaa tcataaaaag taatctgcac
     1561 cctaaaagtt tgatttaacc gccaactgat taaggtgcgg atttttatga caaaataaaa
     1621 atcatctttc cgcagtcatt cccacggacg cgaaagtctg tctttttgaa tcccggataa
     1681 ttttacatcg tcattcccgc gcaggcggga atctagtctg ttcggtttcg gtcatttacg
     1741 ataaattcct attgcttttc atttcctgat tcccacttcc gtgggaatga cggcggagcg
     1801 gataaattct cgcaatctaa aacctcg
//
LOCUS       DAWKST010000225         1768 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.225, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000225 DAWKST010000000
VERSION     DAWKST010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.225"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            52..1560
                     /gene="nadB"
                     /locus_tag="WJL87_001978"
     CDS             52..1560
                     /gene="nadB"
                     /locus_tag="WJL87_001978"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEZ7800368.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASHH
                     AQGGIAAAWSREDDIEKHVADTLEAGAGLCNEAAVRTILSQGKPAIEWLLAQGVAFDR
                     NYDGLHLTREGGHTCRRIAHVADYTGEAVMQSLIAQIRRRPNIRVCERQMALDIQTES
                     GAACGLTVLDCRTQETHRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQAGERFMPHYDRRAELAPR
                     DIVARAIAAETAKQTQDFVSLDISHQPAAFVRQHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPSGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQTVPSKRSSENTSAEADIFSDGIQNTFSRPALQAFNQRHLGILRNDTGLRR
                     AIAQLRLWKQNQTEPHTVSEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
BASE COUNT          422 a          595 c          442 g          309 t
ORIGIN      
        1 aatttaatcc actataccgt ttcagacgac ctctcaaaca aggacaacac catgcaaacc
       61 gattgcgacg tattgattgc cggaaacggg ctggcggcac tgacgctcgc cctgtcgctg
      121 cctgaatcgt tccgcatcgt cattttgtgc aaaaaccggc tggacgacac cgccagccat
      181 catgcgcaag gcgggattgc ggcggcgtgg tcgagagagg acgacatcga aaaacacgtt
      241 gccgatactt tggaagcggg cgcaggtttg tgcaacgaag ccgccgtccg caccatcctg
      301 tcgcagggca aaccggcaat cgaatggctg ctggcgcagg gcgtggcgtt cgaccgaaat
      361 tatgacggtc tgcacctgac acgcgaaggc gggcacacct gccgccgaat cgcccacgtc
      421 gccgactaca cgggcgaagc cgtcatgcag agcctgattg cccaaatacg ccgccgcccg
      481 aacatccgcg tttgcgagcg gcagatggcg ttggacattc aaaccgaatc aggcgcggcg
      541 tgcggactga ccgtcctcga ctgccgaacg caagaaaccc accgcatccg cgcccgccat
      601 accgtactcg caggcggcgg cttggggcag atttacgccg ccaccaccac gccgcccgaa
      661 tgcacgggcg acgccatcgc tatggcgata cgcgcaggct gcgcagtcga aaacctcgaa
      721 tttatccaat tccaccctac aggcttggca aggccgtctg aaaacggccg caccttcctg
      781 atttccgaag ccgtgcgcgg cgaaggcggc atcctgacca accaagcggg cgaacggttt
      841 atgccgcatt acgaccgccg cgccgaactc gcaccgcgcg acatcgttgc ccgcgccatt
      901 gccgccgaaa ccgccaaaca aacgcaagac ttcgtctcgc tcgacatcag ccatcaaccc
      961 gcagcgttcg tccgccagca ttttccgtcc atccatcgac actgtttatc ccaatgcggc
     1021 ttggacatca cgcgccaagc catccccgtc cgccccgtgc aacactacac ctgcggcggc
     1081 atccaaaccg accccagcgg cagaacttcc ctgccgcagc tctacgcctt aggcgaaacc
     1141 gcctgcacag ggttgcacgg agccaaccgc ctcgccagca actccctact cgaatgcgtc
     1201 gttaccgcca ggcttgccgc ccaaaccatc gcggatggac aagcgttcca aaccgtaccg
     1261 tccaaaaggt cgtctgaaaa cacctccgcc gaagcagaca tcttttcaga cggcatccaa
     1321 aacacattca gccgccccgc cctgcaagcg ttcaaccaac gccatctcgg catcctccgc
     1381 aacgataccg gcctgcgccg cgccatcgcc caactgcggc tttggaagca aaaccaaacc
     1441 gaaccgcaca ccgtgtccga atacgaaaac cgcaacctgc tcgaatgcag cctcgccgtc
     1501 gcccaagccg catacaggcg gagacagaac atcggcgcgc attttaatag tgattgttaa
     1561 ggttggctgg ttagaaaaaa tcgttcaaat gaattttaat agcatggtta agagattggc
     1621 attcagtttt tagggttgcg taagttgggt tatcaatctg ttctaaaagc agaaaggccg
     1681 agacctttgc aaaattcccc aaaatcccct aaattcccac caagccattt aggggatttt
     1741 ccatgagcac cttcttccgg caaaccgc
//
LOCUS       DAWKST010000226         1747 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.226, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000226 DAWKST010000000
VERSION     DAWKST010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.226"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            35..922
                     /gene="prmA"
                     /locus_tag="WJL87_001979"
     CDS             35..922
                     /gene="prmA"
                     /locus_tag="WJL87_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ7800369.1"
                     /translation="MPYQQITINVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQIAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEEGWARLSGTKR"
     gene            940..1503
                     /gene="orn"
                     /locus_tag="WJL87_001980"
     CDS             940..1503
                     /gene="orn"
                     /locus_tag="WJL87_001980"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ7800370.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAVHQSDELLDNMDEWNTATHGRTGLTQRVRESLHTEAEVEQKLLDFMSEWVPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPSVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
BASE COUNT          468 a          538 c          433 g          308 t
ORIGIN      
        1 aattcaatcc actatatttc caagaaagcc cgttatgccc taccaacaaa tcaccatcaa
       61 cgtcaacgat gccgtcgccg aacgcctcgc cgacgcgctg atggaacacg gcgcactctc
      121 cgccgccatc gaagatgcct acgccggcac gcaaaacgaa caggcgattt tcggcgaacc
      181 cggtatgccc gccgaacaaa tctggcagca gagcaaagtc atcgccctgt tcggcgaaca
      241 cgacgaagcc gccgccatca tccaaatcgc cgcacaagaa tgcgggttaa aagacttggc
      301 atacaccggc gaaaccatcg aagaccaaga ctgggtgcgt ctcacgcaat cgcaattcga
      361 ccccatccga atttccgacc gcctgtggat taccccctct tggcacgaag cccccgaagg
      421 cactgccgtc aacctacgcc tcgatcccgg cctcgccttc ggcactggca gccacccaac
      481 cacgcgcctc tgcctcaaat ggctggatac gcaactcaaa aacggcgaaa gcgtcctcga
      541 ctacggctgc ggttcgggca tcctgaccat cgctgccctc aaactcggcg caggttctgc
      601 cgtcggcgtc gatattgacg aacaagccgt ccgcgccggc aaggacaacg ccgaacaaaa
      661 caacgtcgat gcacaattct tcctgcccga cggcctgcct caagggcaat tcgacgtagt
      721 tgtcgccaac atcctcgcca accctttacg tatgctcggc gaaatgctcg ccgcccgcac
      781 caaacaaggc ggccgcatcg tgttatccgg tttgttggac gaacaagccg aagaactcgg
      841 cggcatttac agccaatggt tcgatctcga cccggcggaa accgaagaag gctgggcgcg
      901 cttgagcggg acaaaacgct gaaacggaaa ggaaacaccg tgcaggacaa aaacaacctc
      961 tgctggctcg atatggaaat gacggggctt aatcccgaaa ccgaccgcat tatcgaagtc
     1021 gcgatgatta ttaccgactc ggatttgaat gtattggcgc aatccgaagt ttacgccgtc
     1081 caccaaagcg acgagctgct cgacaatatg gacgaatgga acaccgccac acacggtagg
     1141 acggggctga cacagcgcgt gcgcgaatcg ttgcataccg aagccgaagt cgaacagaaa
     1201 ctgctggact ttatgtcgga atgggtaccc ggacgcgcca cgccgatgtg cggcaactcc
     1261 atccaccaag accggcgttt tatggtcaaa tatatgccga aactggaaaa ctacttccac
     1321 taccgcaacc tcgacgtttc cacgctcaaa gagcttgcca aacgctggaa tccgtccgtt
     1381 gccaaaagcg tcgtcaaacg cggttcgcac aaggcattgg acgacatttt ggagagcatc
     1441 gaagaaatgc gccactaccg cgaacacttt ctgatttccg ccccgagagc cgaagcgcaa
     1501 taagaaacaa aaaatgccgt ctgaagcgca aatcctgcat ttcagacggc atttttacag
     1561 cagattgaaa tcaaaaatat acacgcccgt cattcccgcg caggcgggaa tccggaaggt
     1621 cgggcctgcc gttattttca atcattacag aaactgaaag gtctggattc ccgcctgcgc
     1681 gggaatgacg ggcgtgtgca ttcttatagt ggattaacaa aaatcaggac aaggcgacga
     1741 agccgca
//
LOCUS       DAWKST010000227         1732 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.227, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000227 DAWKST010000000
VERSION     DAWKST010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.227"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            356..1660
                     /locus_tag="WJL87_001981"
     CDS             356..1660
                     /locus_tag="WJL87_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ7800371.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDATAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          355 a          431 c          510 g          436 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggattggat gcgatatttc catattcaaa caagggattt gtttcacgca cagggcggca
      241 catcggcaaa atccccgcgc cgccccggtc cagcagggct tgcgtccctc ccggacaagc
      301 cccgaccccg cctttccgaa agacgggctc aaccattaag gaaactttaa tcaaaatgaa
      361 aaaacacata tgggcggcat ctttgctgcc ggcatcccta tcggcagaac ctttaaactg
      421 gtggaagcct tattccgccg tcaattcggg cgataccgcc tgggtgatga ctgcggctgc
      481 cttggtactg ttgatgacgc ttcccgggct ggctttattc tacggcggta tggtgcggaa
      541 aaaaaacctg ctctcgacga tgatgcacag cttttccatc gcgacattgg tgggcattct
      601 ttgggtcgcc gtcggctatt ctttagcgtt cacgccggga aatgccttta tcggcggttt
      661 ggggcgtgta tttttaagcg ggatgcagat agacgctacc gcacagatgc tgaccgtgtc
      721 gcccaatgcg ccgactgttc ccgaaccggt atttatgttt tttcagatga cgtttgccat
      781 tatttcgacc gccattatta ccggcgcgtt tgccgaacgg atgaaatatt cggcaatgat
      841 gctgttttcg ggcatatggt ttttattggt ttatgtgccg ggcgcgcatt gggtgtgggg
      901 cggcggcttt atgagcaagg gcggcgtatt ggattatgcc ggcggtacgg tggtgcacat
      961 caatgccggt atcgcgggac tcgtcgccgc cttggttttg ggcaggcgca taggctacgg
     1021 gcgcgaggcg atgcctccgc acaatatggc gatgacactg atcggcgcgg caatgttgtg
     1081 gttcggctgg ttcggcttta acgccggatc ggcgcttgcg gcagacgcgg cggcgggtat
     1141 ggcgatggcg gtaacgcagg tgtcggccgt attcggcgcg gcaggctggc ttgcctgcga
     1201 aaaaatagcg ggacacaaac cttccgcttt ggggctggct tccggcgcgg tttccggtct
     1261 ggtcggcatc acccctgccg ccggctttac cggcccgtcg ggcgcggccg ccatcggtat
     1321 attgactgcc gccgcgtgct ttgtgtccgt caccgtcgtc aaacacaaat tgcgttacga
     1381 tgattctttg gacgctttcg gcatacacgg attcggcggg ctggtgggcg gaatattgac
     1441 cggcatcttt ttcgacaacc gcattttcgg cggggatgcg gcagtttggc agcagttgtg
     1501 gatacaggta aaagacgggt tcgttatggc ggcatacagc gggctgatga gttgggcgat
     1561 tttgaaggtc gtggggaaaa tctgcggcgg cctgcgcgtc ggcaaggatg tcgaacgcga
     1621 aggtttggat ctgaatatcc acggcgaacg cgtggaataa gggcggttat gccgtctgaa
     1681 gcctgaaaat cgggtttcag acggcatttt tcacgtttgc cgccgatgga ta
//
LOCUS       DAWKST010000228         1724 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.228, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000228 DAWKST010000000
VERSION     DAWKST010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.228"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..365
                     /locus_tag="WJL87_001982"
     CDS             <1..365
                     /locus_tag="WJL87_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ7800372.1"
                     /translation="EYDENSFIGKWYDDGVWDDEEYWKLENDLIEVRKKYPYPMDIPR
                     DIVIGIGTIIDFLMVQNWKLFEIKASPWLPKSVKINERYERFRVMLRYIFTDLDVEDW
                     KFFYFPIQNSKGRLRRKV"
     gene            387..764
                     /locus_tag="WJL87_001983"
     CDS             387..764
                     /locus_tag="WJL87_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ7800373.1"
                     /translation="MCEFKDIIRNVPYFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRYVVIGIGTIIDFLMVPNWKLFEIKASPWLPDSVGINERYERLK
                     TMLRYIFTEKDIVNVRFDYYNKK"
     gene            800..1285
                     /locus_tag="WJL87_001984"
     CDS             800..1285
                     /locus_tag="WJL87_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pretoxin"
                     /protein_id="HEZ7800374.1"
                     /translation="MRLSDGIGNSQTLISNRIHSFYSGGKWIKTEDLKAGIRLLSESG
                     KTQTVRNIVVKPKPLKAYNLTVADWHTYFVKGNRAETEGVWVHNACPPKRTGSSKNEK
                     HGDDGRSQISAESRIAELKNKIIPGMPKNERLKIEQKIKNITKNANRKAKGEEHGRRG
                     R"
     gene            1266..1721
                     /locus_tag="WJL87_001985"
     CDS             1266..1721
                     /locus_tag="WJL87_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800375.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITQYKNILETFMYEPPDEF
                     GTEYYESPDSNLLISVEKNKIISIFCYQELYFMGVNIIGLNFEDFKQLFHHPKSYGVD
                     KYYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIIAGGKDNYSTEPYYDE"
BASE COUNT          598 a          275 c          359 g          492 t
ORIGIN      
        1 aggagtatga cgaaaattca tttattggca aatggtatga tgacggggtg tgggatgatg
       61 aagaatattg gaaattggag aatgatttaa ttgaggttag aaaaaaatat ccttatccga
      121 tggatatacc aagggacatc gtgattggaa tcggtaccat tattgatttt ttaatggttc
      181 aaaattggaa actttttgaa attaaagctt ctccttggtt gcctaaaagt gtcaaaatta
      241 atgagcgtta tgaaaggttc agggtaatgc tccgttatat ttttactgat ctagatgtag
      301 aggactggaa atttttttac tttccaatac aaaatagtaa aggtagattg agacgaaaag
      361 tttaattata ttggagatac attattatgt gtgagttcaa ggatattata agaaacgttc
      421 cttattttga ggggtatgac gaaaattcat ttattggcaa atggtatgat gacggggtgt
      481 gggatgatga agaatattgg aagttggaga atgatttaat cgaggttaga aaaaaatatc
      541 cttatccgat ggacatacca agatatgttg tcattggaat cggtaccatt attgatttct
      601 taatggttcc aaattggaaa ctttttgaaa ttaaagcttc cccttggcta cctgatagcg
      661 tgggaattaa cgaacgttat gaaaggctta aaacaatgct ccgttatatt tttaccgaga
      721 aagacatagt caacgtgcga tttgattatt acaacaaaaa atagcaaaac ccggcaatca
      781 aaatgccgtc tgaagcgata tccggctttc agacggcatc ggcaacagcc aaaccctgat
      841 ttccaaccgc atccactcgt tttattcggg cggcaaatgg attaagacgg aagatttgaa
      901 agcgggaatc aggctgttat ccgaaagcgg caaaacccaa accgtccgca acatcgttgt
      961 caaaccaaaa ccgctcaaag cctacaatct gaccgttgcc gattggcata cctactttgt
     1021 caaaggcaat cgggcggaaa cggaaggagt ttgggttcat aatgcgtgtc cgcccaaaag
     1081 aacaggaagc tccaagaatg aaaaacatgg agacgacggt cgaagtcaaa tatcagcgga
     1141 atcaagaatt gctgaattaa aaaataaaat tattcccgga atgcccaaaa atgaacgctt
     1201 aaagattgag caaaaaatca aaaatattac aaaaaatgcc aatcgaaaag caaaaggaga
     1261 agagcatggt cgacgcggtc gttaaaactc ccgaattttt accttttact tctgtaggaa
     1321 tattccgttt tggtgctgat ataacacagt acaaaaacat actggaaact tttatgtatg
     1381 aacctcctga tgagtttgga acagaatatt atgaatctcc cgactcaaat ttactcattt
     1441 ccgtcgaaaa aaacaaaatt atttcgatat tttgttatca ggagctatat tttatgggcg
     1501 ttaatataat aggtctgaat tttgaagatt ttaaacagct tttccatcac cctaaatcat
     1561 acggtgttga taaatattat ttatcaaatg aatcttatcc gacatatgtc tatgagtttg
     1621 atgaaatagg agtacaagct tgggaagcaa aagggaaaat tgttactatc attgccgggg
     1681 gcaaagacaa ttattcgaca gaaccttact acgatgaata acat
//
LOCUS       DAWKST010000229         1718 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.229, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000229 DAWKST010000000
VERSION     DAWKST010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.229"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            574..1389
                     /locus_tag="WJL87_001986"
     CDS             574..1389
                     /locus_tag="WJL87_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ7800376.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEKTDQKAVSAQQAKE
                     QTSFNNPEPMTGFEHTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDMPGQDAYSINL
                     IEISVYYKKTDQGWVLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERL
                     LTYGVKKMPSAYPEYEAYEDKRHIPENPYFHEFYYIKKGENPAIITHWNNRVNQAEED
                     NYSTSVGSCINGFTVQYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNSSKK"
     gene            1406..>1718
                     /locus_tag="WJL87_001987"
     CDS             1406..>1718
                     /locus_tag="WJL87_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800377.1"
                     /translation="MNEGEVVLTPEQIQTLRGYASRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKTRLEKFDRVALQHFRQYARLINQNNGR
                     LP"
BASE COUNT          574 a          287 c          349 g          508 t
ORIGIN      
        1 agccccaaaa atataccaaa aacagcaaaa caaattgtaa ggatacgtat aggctttgta
       61 aaggtaaatt gtgaaaaaag cagtttttta aacgaatgaa acggcttcgg gctgaaatat
      121 atgctgatgc cctgttcttc ccgtatttct cgtgtgttgt caaagtgcag gctgctttga
      181 aatcggtatt gccatctatg aaccaccact ttgctttatt tcagcgggct tgagatgtgt
      241 ataagaatat tgttttgaat aaatttaaag aaaatgataa tcgttattga cgatttttaa
      301 aggaaagcgt agagtgccaa ttctatgaag caatacggta agtaacaatg aaaatatcta
      361 ctgcttgggt atagagcata tttcacaacc cgtaactatt cttgcggaaa cagagaaaaa
      421 agtttctctt ctatcttgga taaatatatt taccctcagt ttagttaagt attggaattt
      481 atacctaagt agtaaaagtt agtaaattat ttttaactaa agagttagta tctaccataa
      541 tatattcttt aactaatttc taagcttgaa attatgagac catatgctac taccatttat
      601 caacttttta ttttgtttat tgggagtgtt tttactatga cctcatgtga acctgtgaat
      661 gaaaagacag atcaaaaagc agtaagtgcg caacaggcta aagaacaaac cagtttcaac
      721 aatcccgagc caatgacagg atttgaacat acggttacat ttgattttca gggcaccaaa
      781 atggttatcc cttatggcta tcttgcacgg tatacgcaag acaatgccac aaaatggctt
      841 tccgacatgc ccgggcagga tgcttactcc attaatttga tagagattag cgtctattac
      901 aaaaaaaccg accaaggctg ggttcttgag ccatacaacc agcaaaacaa agcacacttt
      961 atccaatttc tacgcgacgg tttggatagc gtggacgata ttgttatccg aaaagatgcg
     1021 tgtagtttaa gcacgactat gggagaaaga ttgcttactt acggggttaa aaaaatgcca
     1081 tctgcctatc ctgaatacga ggcttatgaa gataaaagac atattcctga aaatccatat
     1141 tttcatgaat tttactatat taaaaaagga gaaaatccgg cgattattac tcattggaat
     1201 aatcgagtaa accaggctga agaagataat tatagcacta gcgtaggttc ctgtattaac
     1261 ggtttcacgg tacagtatta cccgtttatt cgggaaaagc agcagctcac acagcaggag
     1321 ttggtaggtt atcaccaaca agtagagcaa ttggtacaga gttttgtaaa caattcaagt
     1381 aaaaaataat ttaaaggatc ttattatgaa tgagggtgaa gttgttttaa caccagaaca
     1441 aatccaaacc ttgcgtggtt atgcttcccg tggcgatacc tatggcggtt ggcgttattt
     1501 ggctaatttg ggtgaccgtt atgcggatga tgctgctgca attgtcggta aggatgcaaa
     1561 cttaaatggt ttgaatttat ggatgaaaaa aggtgtggaa aacctatggg atgatacggt
     1621 cggtaaaaag acccgtttag agaaatttga tcgggttgca ctgcaacatt tcaggcaata
     1681 tgcgcgtcta attaatcaaa ataatggtag attaccca
//
LOCUS       DAWKST010000230         1679 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.230, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000230 DAWKST010000000
VERSION     DAWKST010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.230"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(119..322)
                     /locus_tag="WJL87_001988"
     CDS             complement(119..322)
                     /locus_tag="WJL87_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEZ7800378.1"
                     /translation="MNGLKADRPSANKANLSIDDVIKQEEIKRISFDMSESLHRELKK
                     YAAENGVTIRVVLNKLVSDLLNK"
     gene            complement(365..772)
                     /locus_tag="WJL87_001989"
     CDS             complement(365..772)
                     /locus_tag="WJL87_001989"
                     /inference="COORDINATES: protein motif:HMM:NF025001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HEZ7800379.1"
                     /translation="MIDGAPQTADLAISAIKASDLVLIPVQPSPYDIWATADLVNLIK
                     QRIKLIDGNLKAAFLINRTIKGTKIGGEIRAALEEYELPVLSTVIVQRIIYPTSAITG
                     STVLDKEPNGEAAKEIQSLANEIINLINIDNHI"
     gene            complement(785..1009)
                     /locus_tag="WJL87_001990"
     CDS             complement(785..1009)
                     /locus_tag="WJL87_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ7800380.1"
                     /translation="MKVISVLNQKGGSGKTTVATHLARALQLAGNSILLVDSDPQGSS
                     RDWAAVHEEQPVPVIGIDSPTIDRDLKSVV"
     gene            complement(1356..>1679)
                     /locus_tag="WJL87_001991"
     CDS             complement(1356..>1679)
                     /locus_tag="WJL87_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7800381.1"
                     /translation="EGTTVYADKGYDSAENRQHLKEHQLLDGIMRKACRNRPLTETQT
                     KRNRYLSKIRYVVEQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMCLNLLKAANRLS
                     APAAA"
BASE COUNT          465 a          354 c          297 g          563 t
ORIGIN      
        1 atatgtcaag ttttgcaatg aaaagacgtt ctgtttttgg aagaaggtat tttgttattt
       61 ttagataaag ttggatattg ctttgacatt atggtcaaat acattctgcc gtgtgttatt
      121 atttattcaa taaatctgaa acgagtttat tgagcacgac gcgaatagtt acaccattct
      181 cagccgcgta tttcttcaat tctctatgca gagattcaga catatcaaaa ctgattctct
      241 tgatttcttc ttgcttgatc acatcatcaa tagataagtt tgctttgttt gcactaggac
      301 gatctgcttt taatccgttc ataattattt cctacaatat ttaaaaattt aaaataatat
      361 ttcattaaat atgattatct atatttatca aatttattat ctcattcgca agtgattgaa
      421 tttccttagc tgcttctcca tttggttctt tatccagtac agtgctacct gtgatagcag
      481 atgttggata aataatacgt tgcacaatca cggttgataa aacaggtaac tcatattctt
      541 ctaacgctgc tctaatttct ccgccaatct ttgttccttt aatagttcgg ttaattaaaa
      601 aagcagcctt taaatttcca tctatgagtt ttatccgctg cttgattaaa ttaaccaaat
      661 cagcggttgc ccaaatatcg tatggcgacg gttgaacagg gattaaaact aaatcagagg
      721 ctttaatcgc tgatatggct aaatcggctg tttgtggtgc gccatcaata ataacaaaat
      781 ctttttaaac aacgcttttt aaatcacgat caattgtcgg gctgtcaata ccaatcacag
      841 gaacaggttg ctcttcgtga acagcagccc agtccctact gcttccttgt ggatcgctat
      901 caaccaataa gatgctattt cctgctaatt gcaaggcgcg tgccaagtga gttgcgactg
      961 tggttttacc acttccaccc ttttgattta atacggaaat aactttcatg ttagctcctt
     1021 tgaatatcta cgctcattat gcttttaatt aaataatatt ttaatctatt atttaaataa
     1081 ataaaagaat atttacattt tgttttaaat tttagcaaga aaagctgtta aagagattac
     1141 tgtatcaaac tagacttttt tgagatatgt ggcagaaaat gagttgatat tgcgttaaag
     1201 tttaaattga gacctttgca aaaaagccct tcccccaaca gccgaaaccc aaacacaggt
     1261 tttcgtctat ttccgctcct aattactcct gattttaccc aaatgccccc ttaatcctcc
     1321 ccggacgccc cataatcagg catccgggcc gccttttagg cggcagcggg cgcacttagc
     1381 ctgttggccg ctttcaacag gttcaaacac atcgccttca gatggctttg cgcactcact
     1441 ttaatcagcc cgaaataggc tgcccgcgca tagcggaatt tgcggtgcag cgtaccgaag
     1501 ctttgttcga ccacataacg gatcttcgac aaatatcggt tacgtttggt ttgcgtttcc
     1561 gtcagcggac ggttgcggca ggctttgcgc ataatgccgt ccaacaactg atgttctttc
     1621 agatgttgcc ggttttccgc actgtcatag cctttatcgg catagacggt cgtaccttc
//
LOCUS       DAWKST010000231         1624 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.231, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000231 DAWKST010000000
VERSION     DAWKST010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.231"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(20..682)
                     /locus_tag="WJL87_001992"
     CDS             complement(20..682)
                     /locus_tag="WJL87_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ7800382.1"
                     /translation="MIHAVLFDLDGTLADTALDLGGALNTLLARHGLPAKSMDEIRTQ
                     ASHGAAGLIKLGAGITPDHPDYARWRTEYLEEYDRRYAQDTELFDGVNELIAELNQRG
                     IKWGIITNKPMRFTDKLVPKLGFIIPPAVVVSGDTCGEPKPSIKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYIAPEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(695..1522)
                     /locus_tag="WJL87_001993"
     CDS             complement(695..1522)
                     /locus_tag="WJL87_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ7800383.1"
                     /translation="MKLDIYALLERALLSGDPDEKGRLTDEAFAAVQNADGAEANAPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTTEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPITLFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKNTV"
BASE COUNT          306 a          433 c          500 g          385 t
ORIGIN      
        1 tttgtggggc ggatattttt cagggctgta ttttgtccag acattcgagc agatcgagtg
       61 gcgtgcggat gtggaaatcc gcctgccatg agccggtatc gtcttcggga gcgatgtagc
      121 cccattcggc gaggacggtt ttcatgccgg cgttgcggcc tgcttggatg tcgcgttcgg
      181 catcaccgac gtagagtgtg tgttgcgggt cggcgtggat ttgtccgcac gcatacagca
      241 tgggtttgat gctgggcttg ggttcgccgc aggtgtcgcc gctgacgacg acggcgggtg
      301 ggatgatgaa gccgagtttg gggacgagtt tgtcggtgaa gcgcatgggt ttgttggtga
      361 tgatgcccca tttgatgccg cgttggttga gttcggcaat gagttcgttc acgccgtcaa
      421 agagctcggt gtcttgggcg tagcggcggt cgtattcctc cagatattcg gtgcgccatc
      481 gggcatagtc ggggtggtcg ggggtgatgc ctgcgccgag tttgatcagt cctgccgcgc
      541 cgtggctggc ttgggtgcgg atctcgtcca tgctttttgc aggtagtccg tggcgagcga
      601 gcagggtgtt gagtgcgcct ccgaggtcga gggcggtgtc ggcaagtgtg ccgtcgaggt
      661 cgaagagtac ggcgtgtatc atgtgtgttc cttttcagac ggtatttttg ttctgtttca
      721 aaccctgctc gaaatcttcc agcatatcca attcaaagcg gctgaagccc gctttttcgc
      781 gcgcttcgat gttcacatag ccgcggaaga taaacatatc gtaacgggca atcagactgc
      841 ggaacagggt aatcggttcc aaaccgcgtt cgcggcagag gtgttgatac caatggttgc
      901 cgatggcgac atgacccact tcgtcgcggt aaatgatgtc caatacgccg caggtttccg
      961 aatcgccgcg ctgcgccacc ttcgcgcgta tccccggcgt aacgtccagc ccgcgcgctt
     1021 ccaacacgcg cggtaccaac gccatacgca acagcgggtc gtaggcggtt ttgtacgcca
     1081 tatcccacaa atggttgtgc gcctcgaaac tgccgtaatc gaagccgaaa gcgcgcaatc
     1141 tgtcgcgcat caggcggaaa tggtacacct cttccttcgc cactttcacc cagtcgcgga
     1201 caaactgaaa cggcagcgtg cggaaacggt atgccgcgtc caaagctaga ttgacggcgt
     1261 tgaattcgat atgcgcaatc gcgtgcagca tcgccgcata accttcggtt gtgttcattt
     1321 tgcgcggggt cagttgcgac ggcgcgacca aaacaggctt gtccggccgt cccgctcggg
     1381 ggaagtccgc cggcggcgcg tttgcttccg ccccgtccgc attttgaacg gcggcaaacg
     1441 cctcatccgt cagccgtcct ttttcatctg ggtcgcccga aagcagggcg cgttccagca
     1501 aagcataaat atcgagtttc atctcaagtc cgccgtgttc ggaaaacaaa tattatagcg
     1561 ttttaaaaaa acaagatgag gcatataatc tccgcgattc ggcattccgc gcccaaaccg
     1621 tcaa
//
LOCUS       DAWKST010000232         1609 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.232, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000232 DAWKST010000000
VERSION     DAWKST010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.232"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(79..771)
                     /locus_tag="WJL87_001994"
     CDS             complement(79..771)
                     /locus_tag="WJL87_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ7800384.1"
                     /translation="MFHAFILRIYTMIKNISKLINHAVLIGFIPNIVFAYPIFAENPA
                     SYQNIHVRSWKARRDSGIVKQDLDFSCGAASIATLLNNFYGRHYSEAEILDKMDKTQM
                     RASFDDMQRIMPELGFEAQGYALPFEQLVQLKIPVIVYLKYRKNNHFSVLNGINGETV
                     LLADPSLGHVSMSKSQFLSAWKTRDGEMEGKILAIVPKNTDFVRNQMFFNKNPVRQTR
                     FTVEQIQMRQKR"
     gene            complement(1077..>1609)
                     /locus_tag="WJL87_001995"
     CDS             complement(1077..>1609)
                     /locus_tag="WJL87_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800385.1"
                     /translation="DFILTVASQIAYADLPLSLEELLTDKGKFKLESSISYINTERNQ
                     SEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQAEMKETQGE
                     FVPIIAAAAFGGALGAWEYHGANLYNHGKLGTAQGAATAAGIGAATGVAAKQGWLLLP
                     EADWLEIWHGDRVFMH"
BASE COUNT          502 a          319 c          280 g          508 t
ORIGIN      
        1 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat ataaaataaa
       61 tgggcaaaaa tcggtttatt atcgtttttg ccgcatttgg atttgttcta ccgtaaaacg
      121 tgtttgacga acgggattct tattaaaaaa catctgattt ctaacaaaat cagtattttt
      181 tggcacgatg gctaaaattt ttccttccat ttcgccatca cgtgttttcc atgcgctcaa
      241 gaattgtgat ttgctcattg agacgtgccc cagcgatgga tcagccagca aaacagtttc
      301 tccgttaata ccgttcaata ccgaaaaatg gttgttttta cggtatttta aatacacaat
      361 tacaggaatt tttagttgta ccaactgttc aaatggcaaa gcataacctt gtgcttcaaa
      421 acccagttcg ggcattatgc gttgcatatc atcaaaagaa gcacgcattt gggttttatc
      481 cattttgtcc aagatttccg cttcagaata atgtctgcca taaaaattat tcagtaacgt
      541 ggcaatcgaa gccgcgccgc aagaaaaatc caaatcttgt tttactatgc cggaatctcg
      601 ccgtgctttc caactccgta catggatgtt ttggtaagaa gcggggtttt cagcaaatat
      661 agggtaagca aaaacaatat ttgggataaa gccaattaaa accgcatggt ttattaattt
      721 agaaatattt tttatcatag tatatatgcg tagaataaac gcatgaaaca taaaaaaata
      781 aaatcatatg caattttatt gcatgaataa atatgaataa aatagataat gatgggagta
      841 aatacgccat gtattttgga agtttaaatt tattaataat aaaataattt atcgtagcgc
      901 aaataaatcc caaaattgga acgattagaa aaaaaattaa tacacccatc atcatgcaaa
      961 ctctatatta ataaatagct aactaattct attgtggaaa ttagttagct aactaaaagt
     1021 tattaatgat tatttccgag aattgactgc attgttggca gcattggccc caaaacctag
     1081 tgcatgaata cccggtctcc atgccaaatt tccagccaat ccgcctccgg cagcagcagc
     1141 cagccctgtt ttgccgctac tcctgttgcc gcaccgattc ctgccgcagt agccgcgcct
     1201 tgcgcagttc ctaatttacc atgattatac aaattagcac catgatactc ccatgcacct
     1261 aatgcaccgc caaaagcagc ggctgcaata atgggaacaa attcaccttg tgtttctttc
     1321 atttcagcct gtgataattg aattgctttc acattttggc tgtcaaaaac ttggctgtct
     1381 aaattttgcg ccattacagg tgtaatcatc atagccatta cagttgcaat tttcgttgcg
     1441 ctggtttgca cataaatagg attagcaaat tcgctttgat tgcgttcagt gttgatgtag
     1501 ctaatactgc tttctagttt gaatttaccc ttgtcagtca ataattcttc caaacttaaa
     1561 ggtaagtcag cataagcaat ttggctggca acagtcaaaa taaaatcta
//
LOCUS       DAWKST010000233         1578 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.233, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000233 DAWKST010000000
VERSION     DAWKST010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.233"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            9..1460
                     /locus_tag="WJL87_001996"
     CDS             9..1460
                     /locus_tag="WJL87_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ7800386.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
BASE COUNT          341 a          375 c          392 g          470 t
ORIGIN      
        1 tgatttatat gctttctttc cttagcggct tattgagctt gggtatagaa gtcttgtggg
       61 taaggatgtt ttcgttcgca gcacagtccg tgcctcaggc attttcattt actcttgcct
      121 gttttctgac cggtatcgcc gtcggcgcgt attttggcaa acggatttgc cgcagccgct
      181 ttgttgatat tccctttatc gggcagtgct tcttgtgggc gggtattgcc gactttttga
      241 ttttgggtgc tgcgtggttg ttgacgggtt tttccggctt cgtccaccac gccggtatct
      301 tcattaccct gtctgccgtc gtcagagggt tgattttccc gctcgtacac catgtgggta
      361 cggatggcaa caaatccgga cgacaggttt ccaatgttta tttcgccaac gttgccggca
      421 gtgcattggg tccggtcctt atcggctttg tgatacttga tttcttgtcc acccaacaga
      481 tttacctgct catctgtttg atttctgctg ctgtcccttt gttttgtaca ctgttccaaa
      541 aaagtctccg actgaatgca gtgtcggtag cagtttccct aatgttcggc atcctcatgt
      601 tcctactgcc ggattctgtc tttcaaaata ttgctgaccg tccggatagg ctgattgaaa
      661 acaaacacgg cattgttgcg gtttaccata gagatggtga taaggttgtt tatggggcga
      721 atgtatacga cggcgcatac aataccgatg tattcaatag tgtcaacggc atcgaacgtg
      781 cctatctgct accctccctg aagtctggca tacgccgcat tttcgtcgtt ggattgagta
      841 caggttcgtg ggcgcgcgtc ttgtctgcca ttccggaaat gcagtcgatg attgttgcgg
      901 aaatcaatcc ggcataccgt agccttatcg cggacgagcc gcaaatcgcc ccgcttttgc
      961 aggacaaacg tgttgaaatt gtattggatg acggtaggaa atggctgcgt cgccatcctg
     1021 atgaaaaatt cgacctgatt ttgatgaata cgacttggta ctggcgtgcc tattccacca
     1081 acctgttgag tgcggaattt ttaaaacagg tgcaaagcca ccttaccccg gatggtattg
     1141 taatgtttaa taccacgcac agcccgcatg cttttgctac cgccgtacac agtattccct
     1201 atgcataccg ctatgggcat atggtagtcg gctcggcaac cccggtagtt ttccctaata
     1261 aagaactgct caagcaacgt ctctcccggt tgatttggcc ggaaagcggc aggcacgtat
     1321 ttgacagcag caccgtggat gctgcagcac aaaaggttgt ctctcgtatg ctgattcaga
     1381 tgacggaacc ttcggctggg gcggaagtca ttaccgacga taatatgatt gtagaataca
     1441 aatacggcag agggatttaa ccgtcttaaa gggtttcagg caacgcaggt tttaggtaac
     1501 gtcctgctag ttcaaaaaaa ccgcatcaca gcagtcggga caaaatggtt taaacatttt
     1561 gtcccgaatt cttattcc
//
LOCUS       DAWKST010000234         1574 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.234, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000234 DAWKST010000000
VERSION     DAWKST010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.234"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            41..139
                     /locus_tag="WJL87_001997"
     CDS             41..139
                     /locus_tag="WJL87_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HEZ7800387.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(210..1529)
                     /locus_tag="WJL87_001998"
                     /pseudo
     CDS             complement(210..1529)
                     /locus_tag="WJL87_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239425.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
BASE COUNT          288 a          430 c          507 g          349 t
ORIGIN      
        1 tcttgccgga tgctgcaaac aacgccggtt tgcattcctg atggcggtgg ttttcttaga
       61 cgaacgcccg aacacgcagg aatggatagg cttggggctg gttacggcgg gcgtgttgac
      121 gctggcactg aaacggtaaa gccgcaagaa ataaatgaaa tgccgtctaa aaaactgttt
      181 tcagacggca ttttcgtttc tgtccatcct cagcactcga ccacgcgcac ggatacgggg
      241 acggcttttt tccggagcgt ggcggcgagt ccggcaagcg aggtttcttt gtaggtcgcc
      301 ttcatatccc ggccggtttg cagcatggtt cggatgactt cgtcgagcga gacttttttg
      361 tccgtgccgt cttccaaaag cgcgagcgtg ccgagtttga gggctttttc ggcggcgatg
      421 ccgttgcgct cgatgcaggg gatttgcacc agtccgccga cggggtcgca agtcagcccc
      481 aaatggtgtt ccatcgccat ttcggcggcg ttttccactt gtttgggcgt gccgccgatg
      541 acttcggcgt atgcgcccgc cgccatcgaa cacgctacgc cgacttcgcc ctgacagccg
      601 acatccgcac cggaaatgga ggcgttggtc ttgtagagga tgccgattgc gcctgcggtg
      661 agcaggaagt tttcgacgcg ttcctgtgtg gcgtgcggat tgaacttgcg gaaatagtgc
      721 aatacggcgg gaatgatgcc tgccgcgccg ttggtcggtg cggtaacgac gcgtccgccg
      781 gcggcgtttt cttcgttgac cgccatggcg tacaccatcg gccagagctg ggtgttgacg
      841 atttcggttt cgcgcaggac tttgagcttg gcggcaagct gcggggcgcg gcggcggacg
      901 ttcaatcagc cgggcagttc gccgtccgca cccaagccgc gtttgatgca gccttccata
      961 acctcggcaa cggcagcggc gcggcggcgg atttcggctt cgccgcatcc ggcaagcgcg
     1021 gcttcgtttg ccaacacgac ttcggagatg tcgagccggt tcagacggca tcgggcaagc
     1081 agttcggcgc aactggtata gggataggga acggcttttt ccgtttccgc ctgccggtca
     1141 aaatcttctt cggtaacgac aaagccgccg ccgaccgaat aataaacctg ttcattcaat
     1201 accgtgccgt ctgaagcata ggcggtaaaa cgcaggctgt tggggtgttt gggcagcact
     1261 tgattgccga gtatgttcag gtcgcggtcg gggatgaagc ggatttcttg cccgttgagc
     1321 cggaggatgt gctgcgtgcg gatgcgttcg aggcgttcgg gaatgccggc aagcgggatg
     1381 tcgtgcggca ggctgccttc caaaccgagc atcagcgcgt caaatgtacc gtgtccgtat
     1441 ccggtcagtg cgagcgagcc gtaaatgtcg atgacgatgc gaacagcctg tgcatccaaa
     1501 cctgccgcaa aggcggcggc tgccttcatc gggccgaccg tatgcgaact ggaaggcccg
     1561 ataccgattt tgaa
//
LOCUS       DAWKST010000235         1568 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.235, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000235 DAWKST010000000
VERSION     DAWKST010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.235"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            59..1432
                     /locus_tag="WJL87_001999"
     CDS             59..1432
                     /locus_tag="WJL87_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ7800388.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGVNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPKQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPTQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFEAIKELTGNKLLIERIEGFEPRWWEQEGSKP
                     EKTETSEPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRREHQPCALL
                     QPRYGVK"
BASE COUNT          412 a          511 c          371 g          274 t
ORIGIN      
        1 cccatcctat cggtttcccg cttcagacag cataatgtct gaaagaaaga ctacaatcat
       61 gagtaatcca ttttcctctt taggtttggg tacggaactc gtttccgcac tgaccgcgca
      121 aggttacgaa aacccgacgc ccatccaagc cgccgccatt cccaaagcac tcgccggtca
      181 tgatttacta gccgccgccc aaaccggcac aggcaaaacc gccgccttta tgctgccctc
      241 gctcgaacgc ctcaaacgtt acgccaccgc cagcacctcg cccgcgatgc accccgtgcg
      301 tatgctcgtg ttgaccccga cgcgcgaact cgccgaccaa atcgaccaaa acgtgcaggg
      361 ctacattaaa aacctgccgc tgcggcatac cgtcctcttc ggcggtgtca acatggacaa
      421 acaaaccgcc gacctgcgcg ccggctgcga aattgtcgtc gccaccgtcg gacggctgct
      481 cgaccatgtg aaacagaaaa acatcagttt aaacaaagtc gaaatcgtcg ttctggacga
      541 agcagaccgc atgttggata tggggttcat cgacgacatc cgcaaaatca tgcagatgct
      601 gcctaaacaa cgccaaaccc tgctcttttc cgccactttc tccgcaccga tacgcaaact
      661 ggcgcaagac ttcatgaatg cgcccgaaac cgtcgaggtt gccgcccaaa acaccaccaa
      721 cgcaaatgtc gagcagcaca tcatcgcggt cgataccatt cagaagcgca acctgctcga
      781 acgcctgatt gtcgatctgc atatgaacca ggttatcgtc ttttgcaaaa ccaaacaaag
      841 cgttgaccgc gtaacgcgcg aactggtgcg ccgcaacctg tccgcacagg cgatacacgg
      901 cgaccgttct caacaaagtc ggctcgaaac actcaacgcc ttcaaagacg gcagcctgcg
      961 cgtcctcgtc gccaccgaca tcgccgcacg cggtctggat attgccgaac tgcccttcgt
     1021 catcaattac gaaatgccca cccagcccga agactacgtc caccgcatcg ggcgcacggg
     1081 gcgcgccggt gcggacggcg tggcgatttc cctgatggac gaatccgaac agaaaatgtt
     1141 cgaggccatc aaagaattga ccggcaacaa gctgctcatc gaacgcatcg aaggcttcga
     1201 gccccgatgg tgggaacagg aaggctcaaa accggaaaaa accgaaacaa gcgaaccgag
     1261 acaacgcaac cgctacgaat ctgccagggc tcagcgcgaa aaaaataccc gaccggaaac
     1321 cacggcaaac gatgcgggtg ccgcatgcgg aaaaatcgcc ggtcgcagcc gccgcagccg
     1381 tcgggaacac cagccgtgcg ccctgctcca accgcgttac ggcgtaaaat agccctgaaa
     1441 atcaaatgcc gtctgaacat ttccgtttca gacggcattt ttcaaaccgg actgacgcat
     1501 tgggcgcaac cgcccatacc ggataaattt cttccacaaa cagtttcaga cggcatttgc
     1561 tgcctgta
//
LOCUS       DAWKST010000236         1545 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.236, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000236 DAWKST010000000
VERSION     DAWKST010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.236"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            210..581
                     /locus_tag="WJL87_002000"
     CDS             210..581
                     /locus_tag="WJL87_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800389.1"
                     /translation="MNITLGRNHQINCDEKDLYKFIGYLANHPNDVNLVFEKNSVQGA
                     WGDEGRIQFFSSKAQNIFVPLGFKFTAGVGNIAYRLNCNELFEMLSQLDFVSGGKQNL
                     STIKANIPSQFHAEFDAGANM"
     gene            complement(645..1535)
                     /locus_tag="WJL87_002001"
     CDS             complement(645..1535)
                     /locus_tag="WJL87_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ7800390.1"
                     /translation="MLSYCGVLSEEKFSRYYRYGVQNNKILQYIANRERNALNFIQAF
                     SEKLLKDSGIYPKFADFFAQPNKNTFESMKTAFTDLVIQNTPKNTEVEVRRIFTKIIN
                     PLAYKHNTFGTRKGSISNTPITLDELYYNRLNWRDKGKEKSLTRKEAQTLFADSANAA
                     NLNYLVNKATKFVKTLHKTSEVQRFDPTEANQAHHIFMASEFPDLASLPENLICLTPN
                     QHFNLAHPSNKTTVIDKHYQRICLMAKLDSIEQDNRANTGNYDYHEFIHVLNTGFNTD
                     QFDVSMSYETLKHRILMFDF"
BASE COUNT          475 a          231 c          302 g          537 t
ORIGIN      
        1 gggtttcggc tgtcagcgaa aaggtgaaga gaggtacttt gcaaaggtct caacctgaat
       61 atgtcaacca aatcagcgac aagctcggag aattttttgg ccggacgttg aaataggagt
      121 ttcaattctt ttaattcagc aatagatact gataaaatga aaatcttgag gaatatatca
      181 ttaaaattaa tttgttaagg agataattaa tgaatattac attaggtcga aatcatcaaa
      241 tcaattgtga tgaaaaggac ctttacaagt ttatcggcta tttggcaaat catcctaatg
      301 atgtaaattt agtctttgag aaaaatagtg tgcaaggtgc ttggggtgat gagggacgta
      361 tacagttttt ctcaagtaaa gcacaaaata tttttgtgcc actaggattt aaatttacgg
      421 caggcgtagg taatattgca tataggctaa attgtaatga gctttttgaa atgctatctc
      481 agttagattt tgtttctggt ggaaaacaaa atctttcaac aataaaagca aatattccgt
      541 ctcaatttca tgcagaattt gatgctggtg cgaatatgta aattctaatt taaaacacga
      601 atccgcttca tgagattcgt gttttttatt tttaaaatat ttatttaaaa atcaaacatt
      661 aaaatacgat gcttcaatgt ttcgtaactc atagaaacat caaactgatc tgtattaaat
      721 cctgtattga gtacatgaat aaattcatgg taatcgtaat ttcctgtatt agcacggtta
      781 tcttgctcaa tactgtccaa tttcgccatc aggcaaatac gctggtaatg tttgtcaata
      841 acggtggttt tattgcttgg gtgtgctaaa ttgaaatgct ggtttggtgt caggcaaatc
      901 agattttcag gcagcgatgc taaatcagga aattcgcttg ccataaaaat atgatgtgcc
      961 tgattggctt ctgtgggatc aaaacgttga acttcgctgg ttttgtgtag cgttttgaca
     1021 aattttgtgg ctttattaac taaataattc aaatttgcgg catttgcact atcggcaaat
     1081 agagtttgag cttctttgcg agtgagcgat ttttctttgc ctttatctcg ccagtttagg
     1141 cgattgtaat aaagctcgtc taatgtaatg ggggtattgg aaatactccc ttttcttgta
     1201 ccaaaagtat tatgtttata agccaatgga ttaatgattt tggtaaaaat acggcgaact
     1261 tctacttcgg tatttttggg tgtgttttgg ataacaagat cggtaaacgc cgttttcata
     1321 ctttcaaacg tatttttatt aggctgggca aaaaaatcgg caaattttgg ataaatacca
     1381 ctgtctttta ataatttttc actaaatgct tggatgaaat ttagggcatt tctttcacga
     1441 ttggcaatat attgcaggat tttattattc tgtacgccgt accgatagta tctagaaaac
     1501 ttttcttcgc ttaatacgcc acaatagctc agcattttaa tcggt
//
LOCUS       DAWKST010000237         1528 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.237, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000237 DAWKST010000000
VERSION     DAWKST010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.237"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            180..509
                     /locus_tag="WJL87_002002"
     CDS             180..509
                     /locus_tag="WJL87_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7800391.1"
                     /translation="MGSLIIEDLQESFGKEAVKGKEITVHYTGWLEDGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            538..900
                     /locus_tag="WJL87_002003"
     CDS             538..900
                     /locus_tag="WJL87_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ7800392.1"
                     /translation="MASSNVNKEIKDKKLSLWAKRQDGSVKWFCGQPVTRNDTAKDDT
                     VAADTDTAKKIDTKHLPSTCRDDSSTGCIETPRADFKHFQKISRYRVLPESRHMAEKL
                     RHSRAGGNLGLSARKLIG"
     gene            947..1234
                     /locus_tag="WJL87_002004"
     CDS             947..1234
                     /locus_tag="WJL87_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ7800393.1"
                     /translation="MTGFNDAAGVASASDIKGKYVEKVEVKNGVVTAEMKSSGVNKEI
                     QGKKLSLWAKRQDGSVKWFCGQPVARNDKADTDKIDTKHLPSTCRDAASAD"
BASE COUNT          443 a          410 c          373 g          302 t
ORIGIN      
        1 aaatttaatc cactatattt ccatgaaaaa gcatggaacc tttacgaaaa aaataaaaca
       61 gtcaaacaaa cacagccgtt tcagctgcac cggagcagct gacgtttgag cggcatttga
      121 cttcgccgga cggtatgtta cccttgccgt tttaaccaac aagcaacgaa aggacaaata
      181 tgggcagcct gattattgaa gatttgcagg aaagcttcgg aaaagaagca gttaaaggca
      241 aagagattac cgtgcattac acaggttggc tggaagacgg caccaaattc gactccagcc
      301 tcgaccgccg ccagccgctg accatcacgc tcggcgtcgg acaagtcatc aaaggctggg
      361 acgaaggctt cggcggaatg aaggagggcg gcaaacgcaa gctgaccatc ccttcggaaa
      421 tgggctacgg cgcacacggc gccggcggcg tgattccccc gcacgccact ttgatatttg
      481 aagtcgagct gctgaaagtg tacgaataaa gctgcctggg caataccgtc tgacataatg
      541 gcttcaagca acgtaaacaa agaaatcaaa gacaaaaaac tctccctgtg ggccaagcgt
      601 caagacggtt cggtaaaatg gttctgcgga cagccggtta cgcgcaacga caccgccaaa
      661 gacgacaccg tcgccgccga caccgacacc gccaagaaga tcgacaccaa gcacctgccg
      721 tcaacctgcc gcgatgattc atctaccggt tgcatagaaa caccacgcgc cgacttcaaa
      781 cacttccaaa aaatcagccg ttaccgagta ttgcccgaat cacggcacat ggccgaaaaa
      841 cttcgtcatt cccgcgcagg cgggaatcta ggtctgtcgg cacggaaact tatcgggtaa
      901 aacggtttct tgagattttg cgttctagat tcccactttc gtgggaatga cgggatttaa
      961 tgatgccgcc ggcgtggcat ccgcctccga catcaaaggc aaatatgttg agaaagttga
     1021 agtcaaaaac ggcgtcgtta ccgccgaaat gaaatcaagc ggcgtaaaca aagaaatcca
     1081 aggcaaaaaa ctctccctgt gggccaagcg tcaagacggt tcggtaaaat ggttctgcgg
     1141 acagccggtt gcgcgcaacg acaaagccga caccgacaaa atcgacacca agcacctgcc
     1201 gtcaacctgc cgcgacgcag catctgccga ttaaggcaaa ttataccata aattttaaat
     1261 aaatcaagcg gtaaatgatt ttccacggcc actcggatta atccgggtgg cttccttttt
     1321 aaaggtttgc aaggcaagcg gggtcgtccg ttttggtgga aataatatat cgattgcgct
     1381 tcaaggctct gcgcgtacct cattgccgcc cgtttaaaca cggggtttat ctgacaggcg
     1441 cgtaatccgc cctctcattt accgaacaag cgaactggat tcccgcctac tcgggaatga
     1501 cgaagctatc cgtacggaaa cctgcacc
//
LOCUS       DAWKST010000238         1503 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.238, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000238 DAWKST010000000
VERSION     DAWKST010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.238"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            30..1430
                     /locus_tag="WJL87_002005"
     CDS             30..1430
                     /locus_tag="WJL87_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ7800394.1"
                     /translation="MTPSALKKTVLLLGTAFAAASVHASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLSKIDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNDRHSFGAGIIAQHTSAELRKYADWGIKSKAEILTAKPPKPNGVAEAAKIQAD
                     GHADVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADGAAAKAMWS
                     TMLAANGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFE
                     KEKTVVKGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMN
                     SLPDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARF
                     KNHADIIGLQYTYKFK"
BASE COUNT          394 a          482 c          361 g          266 t
ORIGIN      
        1 ccaccgtccg cctttttacg gaaaccgaaa tgaccccttc cgcactgaaa aaaaccgtcc
       61 tgctgctcgg cactgccttt gccgccgcat ccgtccacgc atccggctac cacttcggca
      121 cacagtcggt caacgcgcaa agtacggcaa atgccgccgc cgccgaagcc gccgatgcct
      181 caaccatttt ctacaatccg gccggtttga gcaaaatcga cagcagccaa atttccgtca
      241 acgccaacat cgtgctgccc agcattcatt atgaggcgga ttccgccacc gactttaccg
      301 ggcttcccgt ccaaggttcg aaaagcggca aaatcaccaa aaccacggtc gcgccccaca
      361 tctacggcgc atacaaagtc aacgacaatc tgaccgtggg cttgggcgtg tacgtcccct
      421 tcggctctgc caccgaatac gaaaaagatt ccgtgttgcg ccacaacatc aacaaactcg
      481 gtctgaccag catcgccgtc gaacctgtcg ccgcatggaa actcaacgac cgccattcct
      541 tcggcgcagg catcatcgcc caacatactt ccgccgaact gcgcaaatat gccgactggg
      601 ggattaagag taaagcagag atattgacgg caaaaccgcc caaacctaac ggtgtagccg
      661 aagctgcaaa aattcaggcc gacggacacg ccgatgtcaa aggcagcgat tggggcttcg
      721 gctaccaact ggcgtggatg tgggacatca acgaccgcgc gcgcgtgggc gtgaactacc
      781 gttccaaagt ctcgcacacg ctcaaaggcg atgccgaatg ggcggcagac ggcgcggcgg
      841 cgaaagcaat gtggagtacg atgcttgcag caaacggcta cacggcgaat gaaaaagccc
      901 gcgttaaaat cgttacgcct gagtctttgt ccgtacacgg tatgtacaaa gtgtccgata
      961 aagccgacct gttcggcgac gtaacttgga cgcgccacag ccgcttcgat aaggcggaac
     1021 tggtttttga aaaagaaaaa accgtcgtca aaggcaaatc cgaccgcacc accatcaccc
     1081 ccaactggcg caacacctac aaagtcggct tcggcggttc ttatcaaatc agcgaaccgc
     1141 tgcaactgcg cgccggcatc gcttttgaca aatcgcccgt ccgcaacgcc gactaccgca
     1201 tgaacagcct gcccgacggc aaccgcatct ggttctccgc cggtatgaaa taccatatcg
     1261 gcaaaaacca cgtcgtcgat gccgcctaca cccacatcca catcaacgac accagctacc
     1321 gcacggcgaa ggcaagcggc aacgatgtgg acagcaaagg cgcgtcttcc gcacgtttca
     1381 aaaaccacgc cgacatcatc ggcctgcaat acacctacaa attcaaataa acggcccgcc
     1441 gtttgattgt agaaatgccg tctgaaacag atttccgttt cagacggcat tgttttaggc
     1501 tcg
//
LOCUS       DAWKST010000239         1483 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.239, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000239 DAWKST010000000
VERSION     DAWKST010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.239"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..>1483
                     /locus_tag="WJL87_002006"
     CDS             <1..>1483
                     /locus_tag="WJL87_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ7800395.1"
                     /translation="DIVNSPIVAVGEYLATSANDGMVHIFKQSGGDKRSYNLKLSYIP
                     GTMPRKDIQNTESTLAKELRAFAEKGYVGDRYGVDGGFVLRQVEWKGQNRVFMFGAMG
                     FGGRGAYALDLTKADGSDPTKASLFDVKHDNNNGNNGNNSVKLGYTVGTPQIGKTHNG
                     KYAAFLASGYATKTIDDQQNKTALYVYDLESSGTLIKKIDVPGGKGGLSSPTLVDKDL
                     DGIVDIAYAGDRGGNMYRFDLSSDNPSSWTVRTIFSGNKPITSAPAISQLKDKRVVIF
                     GTGSDLSEDDVDKTDEQYIYGIFDDDTATTGPVNFSGTGGGLLEQVLTEENKTLFLTD
                     YKRSDGSGSKGWVVKLKDGQRVTVKPTVVLRTAFVTIRKYKDNGCGAETAILGINTAD
                     GGKLTKKSARPIVPADNTAVAQYSGHKQTTKGKSIPIGCMEKDNGIVCPNGYVYDKPV
                     NVRYLDEKKTDGFSTTADGDAGGSGTFKEGKKPARNNRCFSGKG"
BASE COUNT          412 a          382 c          401 g          288 t
ORIGIN      
        1 gcgacatcgt caacagcccc atcgtggcgg tcggcgagta tttggctact tccgccaacg
       61 acgggatggt gcatatcttc aaacaaagcg gcggggacaa gcgcagctac aatctgaagc
      121 tcagctacat ccctggaacg atgccgcgca aggatattca aaacaccgaa tccacccttg
      181 ccaaagagct gcgcgccttt gccgaaaaag gctatgtggg cgaccgctac ggcgtggacg
      241 gcggctttgt cttgcgccaa gtcgaatgga aagggcaaaa ccgcgtgttt atgttcggcg
      301 cgatgggctt tggcggcaga ggtgcatacg ccttggattt gaccaaagcc gacggcagtg
      361 acccgacaaa agcctctttg tttgatgtca aacatgacaa taataatggc aataacggca
      421 ataatagcgt gaaattaggc tacaccgtcg gcacgccgca aatcggcaaa acccacaacg
      481 gcaaatacgc cgccttcctc gcctccggtt atgcgactaa aaccattgac gaccaacaaa
      541 ataaaaccgc gctgtatgtg tatgatttgg aaagcagcgg tacgctgatt aaaaaaatcg
      601 atgtacccgg cggcaagggc gggctttcgt cccccacgct ggtggataaa gatttggacg
      661 gcattgtcga tatcgcctat gccggcgatc gcggcgggaa tatgtaccgc tttgatttga
      721 gcagcgacaa tccgtccagc tggactgtac gcactatttt cagcggcaac aaaccgatta
      781 cttccgcgcc cgccatttcc caactgaaag acaaacgcgt ggttatcttc ggcacgggca
      841 gcgatttgag tgaggatgat gttgataaaa cggacgaaca atatatttac ggtatctttg
      901 acgacgatac ggcgacgacg ggtcctgtaa acttcagcgg tacgggaggc gggctgctcg
      961 agcaagtgct tactgaggaa aataaaacct tattcctgac cgattacaag cgatccgacg
     1021 gctcgggcag caaagggtgg gtagtgaaat tgaaggacgg acagcgcgtt accgtcaaac
     1081 cgaccgtggt attgcgtacc gcctttgtaa ccatccgcaa atataaagac aacggctgcg
     1141 gcgcggaaac cgccattttg ggcatcaata ccgccgacgg cggcaagctg accaagaaaa
     1201 gcgcgcgccc gattgtgccg gccgacaata cggctgtcgc gcaatattcc ggccataagc
     1261 aaaccaccaa aggcaaatcc atccctatag gttgtatgga aaaagacaat ggaatcgtct
     1321 gcccgaacgg atatgtttac gacaaaccgg ttaatgtgcg ttatctggat gaaaagaaaa
     1381 cagacggatt ttcaacaacg gcagacggcg atgcgggcgg cagcggaaca ttcaaagaag
     1441 gtaaaaaacc cgcccgcaat aaccggtgct tctccggaaa agg
//
LOCUS       DAWKST010000240         1444 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.240, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000240 DAWKST010000000
VERSION     DAWKST010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.240"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            26..1363
                     /locus_tag="WJL87_002007"
     CDS             26..1363
                     /locus_tag="WJL87_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ7800396.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGQNAVDSFRALRPSTQWLWVGRMGVSACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGIWGEFKVFGKTIFDLWDYVISAVIMPVGALGVA
                     VFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFVNSLDIL"
BASE COUNT          259 a          347 c          391 g          447 t
ORIGIN      
        1 atcttcacat ttcttaataa attttatgag taaccatact tcttggtcgt ccaaaatcgg
       61 tttcgtgctt gctgcggcag gttcggccat cggtttgggc gcgatttgga aatttcctta
      121 tacggcaggc accaacggcg gcgcggtgtt tttcctgctg tttttgatat ttaccgtctt
      181 ggtcgccctg cccgtccagc ttgccgagtt ttatatcggg cgcacaggcg gtcaaaacgc
      241 cgtcgattcc ttcagggctc tgcgtccgag tacgcaatgg ctttgggtcg ggcgcatggg
      301 cgtttccgcc tgctttattt tgctgtcgtt ttacagcgtg gtcggcggat gggtattaaa
      361 ttatgtcgtc cacagtttca cgggggcggt tcataccggc gcggactttg aagccttgtt
      421 cggcgcgacg atttccaatc cggcaggttc gctgtcctat caggcactgt ttatgctgat
      481 tacggtctgg gtggtcaaag gcggcatttc agacggcatt gaaaaggcaa accgttatct
      541 gatgccgggg ctgtttatcc tctttattgc gctggcaatc cgttcgctga cgctgccggg
      601 tgcaatggag ggcgtgtctt tcctgctcaa accgaattgg tcgtacttta aagccgatac
      661 gatgattacg gctttaggcc aggcgttttt tgccttgagc atcggcgttt ccgctatgat
      721 tacctatgct tcgtatttgg gaaaagatca ggatatgttc cgctcggggc atacgattat
      781 gtggatgaac ctcttggttt cgctgttggc aggtttggtg atttttccgg cggtgttcgc
      841 cttcggtttt gaaccgagcc aggggccggg attgattttt atcgtattgc cggcagtgtt
      901 tatgaagatg ccgttcggta cggttttgtt tgcggtattt atgctgctgg tcgttttcgc
      961 cacgctgact tcggcgtttt cgatgttgga aacggtcatt gcctcaacca tccgccaaga
     1021 cgagcgcaaa cgcaaaaaac acacttggct tatcggcacg gctattttca ttgtcggcat
     1081 cccgtccgcg ctgtctttcg gcatctgggg tgagtttaag gttttcggca aaaccatttt
     1141 tgatttgtgg gactatgtta tttccgccgt cattatgcct gtcggcgcgt tgggcgttgc
     1201 cgtatttacc gcctggattc aggacaagca gtctgtgtta aaagatgccg gcgcgggcag
     1261 caccgtacca cgggcagtgc tgctgctgtg gctgaatacc ttgcgctacc tcgccccgat
     1321 tgccattatt attgttttcg tcaattcttt ggacatcctt taaaagccat ccaaacagca
     1381 aaaatgccgt ctgaaagcct ttcagacggc atttttgctt caggttcagc ctatttcgtt
     1441 caaa
//
LOCUS       DAWKST010000241         1427 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.241, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000241 DAWKST010000000
VERSION     DAWKST010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.241"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..372
                     /locus_tag="WJL87_002008"
     CDS             <1..372
                     /locus_tag="WJL87_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEZ7800397.1"
                     /translation="RLRRLVLIFVNPLYIIATTVPAQQAFLDAAELMQWSIETEGLGL
                     NVISHKILGKDHAQVEFEAYFRDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPAMKQP
                     CICGSGKKFKACCGKYLKPVA"
     gene            complement(590..1384)
                     /locus_tag="WJL87_002009"
     CDS             complement(590..1384)
                     /locus_tag="WJL87_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ7800398.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSHRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWAEYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTVRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
BASE COUNT          365 a          432 c          326 g          304 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aatccactat acatcatcgc tactaccgtt
       61 ccggcgcaac aggcattcct cgatgccgcc gaactgatgc aatggagtat agaaaccgaa
      121 gggctgggct tgaacgtcat ctcgcacaag atactcggca aagaccacgc ccaagtcgaa
      181 tttgaagcct acttccgaga cggacaacac cgatccgcgc atcacgaact gtccggcttc
      241 gtcaacatcg gcggacaatg gtattttatc gatccgaccg ttccgcatcc tgcgatgaaa
      301 caaccctgca tttgcggatc gggcaaaaaa tttaaagcct gctgcggcaa atatctgaaa
      361 cctgtcgcat aaaaatgccg tctgaacgtt cagacggcat ttttaacgcg caaaaaaaaa
      421 ccattcatac caagggtaag tatgaatggt caatacattg cgggaaaacg tcttacttgc
      481 tgcactgccg aaaagggaga aacggcagcg gtaatcagcg gaaaggattg cacccgaatt
      541 aatgttaaga aacgttaatt gtgaaaatat attaacaaac ctgttgaacc tattggtttt
      601 cccgtatcca cccgacccag cgttcaaaca gcttcggttc gagcgcggca acgaccgagc
      661 gtttgaacac gtgttcacca ctccaaaacc cgtcgccttc caaagtcgtc agcctgccgc
      721 ccgcctcctc gaaaatcaac gcacccgcag catagtccca cagcttctgc ccgccgtgga
      781 cataaacatc ataacgcccg cacgccagat aacaccagtc cagcgtgctg ctgcccatac
      841 tccgcaccgt accgaaaggt gcgagcgtac tcatgcggct ggaaagtttg cccgaacgca
      901 gatatttgat ttccacgccc gcgattgcct cattgagttt tttatccacg aggcgcaggg
      961 gcaggcgcgt cccgtttaaa aacgccccct gcccgcgttc ggcgtaaaaa cattcgccgc
     1021 tgaccgggtt gtagattacg cccaattcgg cgcgcccgtt gcggacaaac gccaccgaca
     1081 cggcaaaatg cggcagcccg ttgacaaaat tgttcgtccc gtctatcgga tcgacaatcc
     1141 acagcccctt ttctcccgaa tattccgccc acaaagccga ctgctcctgc cgcgacattt
     1201 cctcgcccaa catcgggcaa tcgatcagaa gcggcaacgc ggcggcaaaa gccgtctgcg
     1261 cggcaatgtc cgcctcgctc aacatcgaac cgtcttcctt gcggtgagac ggcgtattca
     1321 aaaaacgcgg cataatttcg gtttgcgcga tatggcgcac gactttctgc aaacggtgta
     1381 acacttccta ctgtcctcat attttgaact tgcggcacgc gaacgta
//
LOCUS       DAWKST010000242         1420 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.242, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000242 DAWKST010000000
VERSION     DAWKST010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.242"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            66..1226
                     /locus_tag="WJL87_002010"
     CDS             66..1226
                     /locus_tag="WJL87_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ7800399.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNIQLQLTEPL
                     QNIQQPQVTKRKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGGGASQW
                     GNRESFIGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFKRHDDMSVSV
                     RYDSPEFSGFSGSVQFVPAQNSKSAYTPAHFVQNKQNQPPTLVPAVVGKPGSDVYYAG
                     LNYKNGGFAGNYAFKYAKHANVGRDAFELFLLGSGSDEAKGTDPLKNHQVHRLTGGYE
                     EGGLNLALAAQLDLSENADKTKNSTTEIAATASYRFGNAVPRISYAHGFDFIERGKKG
                     ENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          353 a          359 c          365 g          343 t
ORIGIN      
        1 gaagacgtat cgggtgtttg cccgatgttt ttaggttttt atcaaattta caaaaggaag
       61 ccgatatgcg aaaaaaactt accgcgctcg tattgtccgc actgccgctt gcggccgttg
      121 ccgatgtcag cctgtacggc gaaatcaaag ccggcgtgga aggcaggaac atccagctgc
      181 agttgaccga accgctccaa aatattcaac aacctcaggt tactaagcgc aaaagccgca
      241 tcaggacgaa aatcagcgat ttcggctcgt ttatcggctt taaggggagc gaggatttgg
      301 gcgaagggct gaaggctgtt tggcagcttg agcaagacgt atccgttgcc ggcggcggcg
      361 cgtcccagtg gggcaacagg gaatccttta tcggcttggc aggcgaattc ggcacgctgc
      421 gcgccggtcg cgttgcaaat cagtttgacg atgccagcca agccattgat ccttgggaca
      481 gcaataatga tgtggcttcg caattgggta ttttcaaacg ccacgacgat atgtcggttt
      541 ccgtacgcta cgattccccc gaattttccg gttttagcgg cagcgtccaa ttcgttccgg
      601 cccaaaacag caagtccgcc tatacgccgg ctcattttgt tcagaataag caaaatcagc
      661 cgcctactct cgttccggct gttgtcggca agccggggtc ggatgtgtat tatgccggtc
      721 tgaattacaa aaatggcggt tttgccggga actatgcctt taaatacgcg aaacacgcca
      781 atgtgggccg tgatgctttt gagttgttct tgctcggcag cgggagtgat gaagccaaag
      841 gtaccgatcc cttgaaaaac catcaggtac accgcctgac gggcggctat gaggaaggcg
      901 gcttgaatct cgccttggcg gctcagttgg atttgtctga aaatgccgac aaaaccaaaa
      961 acagtacgac cgaaattgcc gcgactgctt cctaccgctt cggtaatgca gttccacgca
     1021 tcagctatgc ccatggtttc gactttatcg aacgcggtaa aaaaggcgaa aataccagct
     1081 acgatcaaat catcgccggc gttgattatg atttttccaa acgcacttcc gccatcgtgt
     1141 ctggcgcttg gctgaaacgc aataccggca tcggcaacta cactcaaatt aatgccgcct
     1201 ccgtcggttt gcgccacaaa ttctaaatat cggggcggtg aagcggatag ctttgttttt
     1261 gacggctcgc cttcattctt tgattgcaat ctgactgcca atctgcttca gccccaaaca
     1321 aaaatccgga tacggaagaa aaacggcaat aaagacagca aataccgtct gaaagatttt
     1381 cagacggtat ttcgcatttt cggcttggtt tgcacatata
//
LOCUS       DAWKST010000243         1387 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.243, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000243 DAWKST010000000
VERSION     DAWKST010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.243"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..>1387
                     /gene="frpC"
                     /locus_tag="WJL87_002011"
     CDS             <1..>1387
                     /gene="frpC"
                     /locus_tag="WJL87_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated protein FrpC"
                     /protein_id="HEZ7800400.1"
                     /translation="WVSADDGLLVRDLNGNGIIDNGAELFGDNTKLADGSFAKHGYAA
                     LAELDSNGDNIINAADAAFQSLRVWQDLNQDGISQANELRTLEELGIQSLDLAYKDVN
                     KNLGNGNTLAQQGSYTKTNGTTAKMGDLLLAADNLHSRFKDKVELTAEQAKAANLAGI
                     GRLRDLREAAALSGDLANMLKAYSAAETKEAQLALLDNLIHKWAETDSNWGKKSPMRL
                     STDWTQTANGGVALTPSQVTRLKQNALVSLSDKAKAAIDAARDRIAVLDAYTGQDSST
                     LYYMSEEDALNIVKVTNDTYDHLAKNIYQNLLFQTRLQPYLNQISFKMENDTFTLDFS
                     GLVQAFNHVKETNPQKAFVDLAEMLAYGELRSWYEGRRLMADYVEEAKKAGKFEDYQK
                     VLGQETVALLAKTSGTQADDILQNVGFGHNKNVSLYGNDGNDTLIGGAGNDYLEGGSG
                     SDTYVFGKGFGQ"
BASE COUNT          402 a          330 c          331 g          324 t
ORIGIN      
        1 tgggtttctg ccgatgacgg tttactcgtc cgcgatttga acggcaacgg catcatcgac
       61 aacggcgcgg aactcttcgg cgacaatacc aaactggcag acggttcttt tgccaaacac
      121 ggctacgcag ctttggccga attggattca aacggcgaca acatcatcaa cgcggcagac
      181 gccgcattcc aatccctgcg tgtatggcag gatctcaacc aggacggcat ttcccaagct
      241 aatgaattgc gtacccttga agaattgggt atccaatctt tggatctcgc ctataaagat
      301 gtaaataaaa atctcggcaa cggtaacact ttggctcagc aaggcagcta caccaaaaca
      361 aacggtacaa ccgcaaaaat gggggattta cttttagcag ccgacaacct gcacagccgc
      421 ttcaaagaca aagtggaact cactgccgaa caggcaaaag ccgccaatct tgcgggcatc
      481 ggccgtctgc gcgatttgcg cgaagctgcc gcattgtccg gcgatttggc caatatgctg
      541 aaagcttatt ctgccgccga aactaaagaa gcacagttgg cattgttaga taatttgatt
      601 cacaaatggg cggaaaccga ttcgaactgg ggcaaaaaat cgccaatgcg actttcaacc
      661 gattggacgc aaacggctaa cgggggtgtg gctcttaccc cgtctcaagt aacgcgacta
      721 aaacagaacg ctttagtttc tctttccgac aaagccaaag cagctatcga tgccgcccgc
      781 gaccgcattg ccgtgcttga tgcctacacg gggcaggatt ccagcacact ctattacatg
      841 agcgaagaag acgcgcttaa tatcgtcaaa gtaaccaacg atacatacga ccatctcgcc
      901 aaaaacatct accaaaacct gttgttccaa acccgtttgc agccatattt gaatcaaatc
      961 agtttcaaaa tggaaaatga tacgttcact ttggatttta gtggtcttgt tcaagcattt
     1021 aaccatgtca aagaaactaa tccgcaaaaa gcttttgtgg atttggccga gatgcttgca
     1081 tatggcgaac ttcgttcttg gtatgaaggt cgaagactaa tggccgatta tgtggaggag
     1141 gcaaaaaaag caggtaaatt tgaagattac cagaaagtgt tgggtcagga gaccgttgca
     1201 ttattagcta aaacatcggg tacgcaagca gatgatatcc tgcaaaatgt aggctttggt
     1261 cataataaaa atgtttcttt atatggtaat gacggcaacg acactctaat cggcggcgcc
     1321 ggtaatgact atttggaggg cggcagcggt tcggatactt atgtcttcgg caaaggcttc
     1381 ggtcagg
//
LOCUS       DAWKST010000244         1380 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.244, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000244 DAWKST010000000
VERSION     DAWKST010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.244"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(73..996)
                     /locus_tag="WJL87_002012"
     CDS             complement(73..996)
                     /locus_tag="WJL87_002012"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ7800401.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPAFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRNCG"
BASE COUNT          370 a          328 c          341 g          341 t
ORIGIN      
        1 ttttggtttt ctgtttttgt aggaatgatg aaaatttaag ttttaggaat ttaccggaaa
       61 aaacagaaag cgttatccac aatttctgat gttcagctcg tgaaatgcgt cgggcaaatc
      121 atcgctgtcg gcaaattcca cccggtcgta agccgtttcg tctgccaaaa ccgcgcgcaa
      181 gagtgcgttg ttgatggcgt gtcccgattt gtagccttca aacgcgccga caatcggatg
      241 tccgacgata tacaaatcac cgatggcatc aaggattttg tggcgcacaa actcatcggg
      301 atagcgcaag ccttcaggat tcaggacatc cgtgtcgtca atcacgatgg cgttgttcaa
      361 attgccgccc aaacccagat tgtgggcgcg catcatttcc acttcgtgca taaagccgaa
      421 ggtgcgcgcg cgcgcgattt cgtcgatgta ggatttgccg gcgaaatcga tttcaaaagt
      481 gggcgagctg cggttgaaag ccggatggtc gaattcgatg gtcagcgtta ccttaaagcc
      541 gtcatacggc gtaaagcgca cccatttgcc cgcttctttg atttcgacag gcttgaggat
      601 tttcaaaaaa cgcttttgcg ccttttgatc gaccacgccc gcatcttgca aaaggtaaat
      661 aaacggcagg ctggagccgt ccataatcgg gatttcgggt gcgttcagct caatcagcgc
      721 gttgtcgata ccgtaggcgg acagcgcgga cataatgtgt tcgatcgtgc cgacgcgcac
      781 gcctttgtcg gtaacgatgg tggaggaaag gcgggtatcg ttgatcaaat aaggggtcag
      841 cttgatttgt tcgcccatct cgccgtccaa atcggtacgg cggaaggaaa tcccgctgtt
      901 ttcaggcgcg gggtgcaggg tcagtgcgac ccgttcgccg gaatgcaggc cgactccggt
      961 aacgctgatg gatttcgcca aagttctttg cagcataaac cgcttcctta tcaatggggt
     1021 aagttgggga atgatacgat aaaaccggaa aaacaagcta tgtttttcca tagtatttgc
     1081 caatgtatcc gttttcaata cgtaagctgc ataaaaatgt atagtggatt aacaaaaatc
     1141 aagacaaggc gacgaacccg cctccctcct gaaaaacgca aaaaatgccg tccgaaaacc
     1201 tttcggacgg cattttcgcg tgaaccgtca ttcccacaag gacataaaac caaaacagaa
     1261 aaccaaaaac agaaacctaa aattcgtcat tcccacgaaa gtgggaatct agttcgttga
     1321 gtttcagtta tttagaataa attttgaaac ttaaatcccg tcattcccac gaacctacat
//
LOCUS       DAWKST010000245         1346 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.245, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000245 DAWKST010000000
VERSION     DAWKST010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.245"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..>1346
                     /locus_tag="WJL87_002013"
     CDS             <1..>1346
                     /locus_tag="WJL87_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ7800402.1"
                     /translation="KLSKQPEYAYLKQLQTAKNVDWKQVQLAYDKWDYKQEGLTGAGA
                     AIVVIIVTALTYGYGAAAAGSVTAAGSSTAAAATTTAAATTTVSTAAAMQTAALASLY
                     SQAAVAIINNKGDVGKALKDLGTSDTVKQIVTSALTAGALNQMGADIAQLNSKVRTEL
                     FSSTGNQTIANLGGRLATNLSNAGISAGINTAVNGGSLKDNLGNAALGALVNSFQGEA
                     ASKIKTTFSDDYVAKQFAHALAGCVSGLVQGKCKDGAIGAAVGEIVAESILGGRNPAT
                     LSDAEKHKVISYSKIIAGSVAALNGGDVNTAANAAEVAVVNNALNFDSTPTNAKKHQP
                     QKPDKTALEKIIQSIMPAHAAGAMTNPQDKDAAIWISNIRNGITGPIVITSYGVYAAG
                     WTAPLIGTAGKAAISTCMANPSGCTVMVTQAAEAGAGIATGAVTVGNAWEAPVGALS"
BASE COUNT          380 a          323 c          369 g          274 t
ORIGIN      
        1 aagctgtcca aacagcccga atacgcctac ctgaaacagc ttcagacggc caagaacgtc
       61 gattggaaac aggtgcagct ggcctacgac aaatgggact ataaacagga aggcttgacc
      121 ggagccggtg cagcgattgt ggtgattatt gtaaccgctc taacttatgg atacggagcg
      181 gctgcagcgg gtagcgtaac tgccgcagga agtagtacag ccgcagctgc aacaacgaca
      241 gcggcagcaa ctaccactgt ttctactgca gctgccatgc aaaccgcagc tttagcctcc
      301 ttgtatagcc aagcagctgt agccatcatc aataataaag gcgatgtagg caaagcatta
      361 aaagatctcg gcaccagtga tacggtcaag cagattgtta cttccgcact gacggcgggt
      421 gcattaaacc agatgggagc agatattgcc caattgaaca gcaaggtaag aaccgaactg
      481 ttcagcagta cgggcaatca aaccattgcc aaccttggag gcagattggc caccaacctc
      541 agcaatgcag gtatctcagc tggtatcaat accgccgtta acggcggcag cttgaaagac
      601 aacttaggca atgccgcatt gggagcattg gttaatagct tccaagggga agccgccagc
      661 aaaatcaaaa caaccttcag cgacgattat gttgccaaac agttcgccca cgctttggcg
      721 ggttgtgtta gcggactggt acagggaaaa tgtaaagatg gggcaattgg cgcagcagtt
      781 ggggaaatcg tagctgaatc catacttggc ggcagaaacc ctgctacact cagcgatgca
      841 gaaaagcata aagttatcag ttactcgaag attattgcgg gcagcgtggc ggcactcaac
      901 ggcggcgatg tgaatactgc ggcgaatgcg gctgaggtag ctgtggtgaa taatgctttg
      961 aattttgaca gcacccctac caatgcgaaa aagcatcaac cgcagaagcc cgacaaaacc
     1021 gcactggaaa aaatcatcca aagtattatg cctgcacatg cggcaggtgc gatgactaat
     1081 ccgcaggata aggatgctgc catttggata agcaatatcc gtaatggcat cacaggcccg
     1141 attgtgatta ccagctatgg agtttatgca gcaggttgga cagctccgct gatcggtaca
     1201 gctggtaaag cagctatcag cacctgtatg gctaatcctt ctggttgtac tgtcatggtt
     1261 acgcaggctg ctgaagcggg cgcgggaatc gccacgggtg cggtaacggt aggcaacgct
     1321 tgggaagctc cagtaggagc gttgtc
//
LOCUS       DAWKST010000246         1309 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.246, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000246 DAWKST010000000
VERSION     DAWKST010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.246"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            38..461
                     /locus_tag="WJL87_002014"
                     /pseudo
     CDS             38..461
                     /locus_tag="WJL87_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            567..1130
                     /gene="pgsA"
                     /locus_tag="WJL87_002015"
     CDS             567..1130
                     /gene="pgsA"
                     /locus_tag="WJL87_002015"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ7800403.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGTFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1210..1285
                     /locus_tag="WJL87_002016"
     tRNA            1210..1285
                     /locus_tag="WJL87_002016"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1243..1245,aa:Gly,seq:gcc)
BASE COUNT          317 a          312 c          318 g          362 t
ORIGIN      
        1 tcgctgggta tggtttgccg ccataagctg tctgctgttg tccgccgttt ttattgcacc
       61 ttacctgacg gcatttcacg aacaagaaaa aacattcgaa tatgcggaat tgaccgttac
      121 cgcccccaac cgcagcggac gggcaatcaa actggatgca gacgggcagc attaccggct
      181 ttcctgctat gggttcgaca gtttgtgcac aggcggcaat atcggcagaa ccatcagggc
      241 ggagcaggtt agagccgttt taagcgaaac cgtcggcaaa ggttttttaa acggcgttct
      301 gttggaatac cgcaacagtg gcagtgtcta taagcaataa agattttgcc cgcacggaag
      361 accgccttgt cgaagtgttg gcacaacctg ccgttttctg cctgaaactg ggtattttgc
      421 ttttattgcc cgccattttc ctgcgtttga aaagaatgtg aaaacagatt ggcggggcgg
      481 gggaatgccc atgcagcaat tttacgtttt gggttatgag gataaccgtt ataatcacaa
      541 ctttacagtc tgcacagaga aaaccgatgc cttggaatct ccctatcttc ctcacatggt
      601 tgagggtctt gctcattcct gtcatgacag ccctttttta cctgcctttt ccgtggttcg
      661 cggaggaaac gattaatctc accgccgccg tcatttttgc cgttgccgcc ttaaccgact
      721 ggtttgacgg atttttggca agactgtgga aacaaacttc cgatttcggc accttcctcg
      781 atcccgtcgc cgacaagctg atggttgccg tatcgctact tttactggtc aaactcgacc
      841 ggacctatgt tttgtttgcc atgatcatta tcggcaggga aattaccatt tccgcattgc
      901 gcgaatggat ggcgcaaatg ggcaaaagga acagcgttgc cgtcgccacc gtcggtaagt
      961 tcaaaaccgc cgcgcaaatg ctggcaattt tcctgctgct gctgaatatt cccgattttt
     1021 acggatttaa tcttgttgtt atcggcaatg tattgatgtt tatcgcatct ttgctgacag
     1081 tctggtcgat gctgtattat ctgaaaatgg cgtggaaaga aatcgcctga aaaaaacata
     1141 aaaatagctt gacggcaaaa acagaatcca taataattgc gtctcttcga tgttggagaa
     1201 tgaaatcggg cgggaatagc tcagttggta gagcgcaacc ttgccaaggt tgaggtcgcg
     1261 agttcgagac tcgtttcccg ctccaaaaat ttcattttcc cacaatcat
//
LOCUS       DAWKST010000247         1294 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.247, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000247 DAWKST010000000
VERSION     DAWKST010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.247"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..890
                     /locus_tag="WJL87_002017"
     CDS             <1..890
                     /locus_tag="WJL87_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ7800404.1"
                     /translation="TAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVSEAKA
                     NLPKDAQISESELSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNPALKNLSP
                     AQRRDFADNIIAEKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQH
                     ILIKADSENAAVGAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVM
                     VPAFEEAVHALKPGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAE
                     QATVNLLRDLHSGAYVDIR"
BASE COUNT          354 a          381 c          329 g          230 t
ORIGIN      
        1 ccacggcgca aaaagaagcc gcacccgcac aacagcaggg cggtatccgc ttttcagacg
       61 gcattgccgc cgttgccgac aacgaagtca tcacgcgccg ccggcttgcc gaagccgttt
      121 ccgaagccaa agccaacctg cccaaagacg cgcagataag cgaatccgag ctgtcccgac
      181 aggtgctgat gcagcttgtc aaccaatccc tgattgtaca ggcgggcaaa cgccgcaaca
      241 ttcaagcaag cgaagcggaa atcgatgccg tcgtcgcaaa aaatcccgcc ctcaaaaacc
      301 tcagccccgc ccaacgccgc gattttgccg acaacatcat tgccgaaaaa gtccgccagc
      361 aggcagtgat gcagaacagc cgcgtgagcg aagctgaaat cgatgccttc ctcgagcagg
      421 cgcaaaaaca aggcatcacc ctgcccgaag gcgcaccgtt gcgccaatac cgcgcccaac
      481 acatcctgat taaagctgac agcgaaaacg ccgccgtcgg cgcggaaagc accatccgca
      541 aaatctacgg agaggcccgc agcggcacag acttttccag cctggcgcgc caatattcgc
      601 aagacgcgag cgcgggcaac ggcggagatt tgggctggtt tgccgacggc gtgatggttc
      661 ccgcctttga agaagccgtc cacgcgctca aacccggaca ggtcggcgcg cccgtccgca
      721 cccaattcgg ctggcatatc atcaaattga acgaagtgcg cgatgccggc acacctcagg
      781 aacgtatccg caattccgtg cggcaataca tcttccaaca aaaagccgaa caggcaaccg
      841 tcaacctgtt gcgtgacctg cattccggcg cgtatgtcga catccgctaa ggcggtttga
      901 agcaaaaagc cataccgatc ggcaaaaatc cgggcggtat ggctttttgg atttcgagtt
      961 acttttacac cgtcattcat cattcccgcg aaagcgggaa tctagaaacg aaaagtaaca
     1021 ggaatttatc gggaatggct ggaatttaaa ggtctggatt cccgctttcg cgggaatgac
     1081 gggattttgg gttgtggtaa tttatcggaa aaacaaaaaa acctatgccg tcattcccga
     1141 gcaggcggga atccggttat ttaaaactgc agaaatttat ccgaagcaac aacaatcttt
     1201 ccatcgtcat tcccgcgtag gcgggaatct agcaacgaaa agtaacagga atttatcggg
     1261 aaaacagaaa cctctatgcc gtcattcccg cgaa
//
LOCUS       DAWKST010000248         1290 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.248, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000248 DAWKST010000000
VERSION     DAWKST010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.248"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            166..1278
                     /gene="nadA"
                     /locus_tag="WJL87_002018"
     CDS             166..1278
                     /gene="nadA"
                     /locus_tag="WJL87_002018"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ7800405.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETAGRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEADTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYICRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
BASE COUNT          299 a          333 c          387 g          271 t
ORIGIN      
        1 accaaagcgg tttgtcggaa cgaagcgggt cggttttacg gtatcgcaaa cctgttttca
       61 gacgaccttg cttattatac tcaatttgag cataatataa aaatatttca aaatataaac
      121 ggcaaatctt tatgccgtct gaaaaaccag ccataaggaa gtctcatgca aaccgccgcc
      181 cgccgctcgt tcgattacga tatgcccctc attcaaacgc cgacttccgc ctgccaaatc
      241 cgtcaggcgt gggcgaaggt tgccgacacg cccgaccgcg aaacggcagg ccgtctgaaa
      301 gacgaaatca aggctttgct gaaggagaaa aacgcggtct tggtggcgca ttattatgtt
      361 gatccgctga ttcaggattt ggctttggaa acgggcggat gcgtgggcga ttcgctggaa
      421 atggcgcgct ttggtgcgga acacgaagcc gatacattag tggtggcggg cgtgcgtttc
      481 atgggcgaga gcgcgaaaat cctctgccct gaaaaaacag tgctgatgcc tgatttggag
      541 gcggaatgtt ctttggattt gggttgcccg gaagaggcgt tttcggcgtt ttgcgaccaa
      601 caccccgacc gcacggtggt ggtgtacgcc aacacttccg ccgccgtgaa agcgcgtgcc
      661 gactgggtgg taacgtcttc agtggcgttg gaaatcgtgt cgtatctgaa atcgcgcggc
      721 gagaagctga tttggggacc cgaccgccac ctcggcgact acatctgccg cgaaacgggc
      781 gcggatatgc tgttgtggca gggttcgtgc atcgtccaca acgaatttaa agggcaggag
      841 ctggcggcgt tgaaagcgga acatcccgac gcggtggtgc tggttcatcc tgaatcgccg
      901 caaagcgtca tcgaactggg cgacgtggtc ggctcgacca gcaaactgct caaggccgcc
      961 gtatcgcgtc ctgaaaaaaa attcatcgtg gcgaccgatt tgggcatcct gcacgaaatg
     1021 caaaagcagg cgcccgacaa agaatttatc gccgcgccga cggcgggcaa cggtggaagc
     1081 tgtaaaagct gcgcgttctg cccgtggatg gcgatgaatt cgctgggcgg catcaaatac
     1141 gccctgacaa gcggacgtaa cgaaatcctg ttggacagga agctgggcga agccgccaaa
     1201 ctgcctttgc agcgtatgct cgacttcgcg gcaggactca aacgcgggga tgtgttcaac
     1261 ggcatgggtc ccgcctgatt tgccgtccat
//
LOCUS       DAWKST010000249         1277 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.249, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000249 DAWKST010000000
VERSION     DAWKST010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.249"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            57..782
                     /locus_tag="WJL87_002019"
     CDS             57..782
                     /locus_tag="WJL87_002019"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ7800406.1"
                     /translation="MPRFYLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAAQLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLADIRQPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          353 a          393 c          287 g          244 t
ORIGIN      
        1 gtccgtttcc atcacgccgc tgcgacagat tataaccgtc cgaaccgcca aaaactatgc
       61 cccgattcta cctgcccgaa aacctttccg tcggacaaac cgtcgccctg cccgacaaca
      121 tcgtccgcca cctcaacgtc ctacgcgtcc gccccaacga aaacatcacc ctcttcgacg
      181 gcaaaggcaa ggcacacgcc gcacagctga ccgttttgga aaaacgccgc gccgaagccg
      241 aaatcctgca cgaagacaca accgacaacg agtccccgct caacatcaca ctgatacaat
      301 ccatctcctc cggcgatcgc atggatttca ccctgcaaaa aagcgtcgaa ctcggcgtaa
      361 ccgccataca gcccgtcatc agcgaacgct gcatcgtccg cctcgacgga gaacgcgccg
      421 ccaaacgcct cgcacgctgg caggaaatcg tcatctccgc gtgcgaacaa agcggcagga
      481 acaccgttcc ccccgtactg cccatcatcg gctaccgtga agcactcgac aaaatgccgt
      541 ctgaaagcac caagctgatt atgagcatca accgcgcccg caaacttgcc gacatacgcc
      601 aaccgtccgg cgcaatcgtc tttatggtcg ggccagaagg cggctggaca gaacaggaag
      661 aacaacaggc atttgaagct ggctttcagg cggttacact cggcaaacgg attttacgca
      721 cagaaaccgc cccactcgcc gccctcgccg ccatgcagac gctttggggc gatttcgcat
      781 aaacagaaat gccgtctgaa acccgttcag acggcatttt gcagccgatt aagatagtag
      841 gttcaaataa gatttccccc gccgtcattc ccgcgaaagc gggaatctgg aaatttaatg
      901 ttgcggtgat ttatcggaaa tgactgaaac tcaacagact ggattcccgc ctgcgtggga
      961 atgacgagat tttaggtttc tgtttttggt tttctgttct cgcggaaata acgaaatttt
     1021 aagttttagg aatttgtcgg aaaaacagaa atcccccccg ccgtcattcc cgcaaaagcg
     1081 ggaatctaga aacgaaaaac tacagggatt tatccgaaac aacaaaccct ctccgccgtc
     1141 attcccgcgc aggcgggaat ctagaaattt aacgttgcgg tgatttatcg gaaatgactg
     1201 aaactcaacg aaccggattc ccactttcgc ggaaatgaca aagtggaagt tgcccgaaac
     1261 ttaaaacaag cgaaacc
//
LOCUS       DAWKST010000250         1219 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.250, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000250 DAWKST010000000
VERSION     DAWKST010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.250"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(32..490)
                     /locus_tag="WJL87_002020"
     CDS             complement(32..490)
                     /locus_tag="WJL87_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800407.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGTEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
BASE COUNT          434 a          223 c          129 g          433 t
ORIGIN      
        1 atcgattccg ttgtattttc gcaagacacg tttagtctag agtctgtata ttacatttat
       61 ttttagggtc tgctagccaa tttcttgttc ccttcattat tttatcttct gaaagaaaat
      121 tatttttttc catgctatta atattaatga tatgattttt atttaaaata atttttccat
      181 ataaaatttc cggtttgtaa tagcaagagt ctgaagcaaa tccttgcccg tcaatataag
      241 aaaaataaat gttatcatcc tccttataat attctaaaac caatccggat attattttat
      301 caaaattccc ttcagttcca tctttcatga ctaaaatata attagcaggc caacctttat
      361 cgtaaacata ttcaaactga tagttcccga aactctcgat atccgaacta aaaaagaaga
      421 aaagcaaatt aatagccaat acaaaaacaa ataagaacaa aaatagcaaa attttcaact
      481 tagttaacaa tatttacctc tcctttaaat tcaatcctga aaggtacccc ttaccctggg
      541 ctaccaatta tagtttccat atttctaaat attgttttta cattactttt ttctcccccc
      601 aaaggaattc attttaaaat catgcttttc aggtgctaat cgatacttac cattaacatc
      661 tttaatcaca gatatatttc catgtatagc ccaacgtgaa aaatctgagt attatataca
      721 gttatacctt actgaaacaa agctagctgt acatatctgt accatatttt tactacaaac
      781 aaaaaatatt tataattatt aaggataaga taaaaacccc caacgccaga ttaattctta
      841 ataatttcaa ataaaaaata tttgagattt tttcttttcc aaataatata ttgaaatatt
      901 taaagttaag gtagcttgat ttaagtagaa aatcaaaaaa accattaatt aatttttctt
      961 ctttaaatat tttatatgat attataaaat taaataacat tgcgaaaaca tatgaaatca
     1021 tcaaaatacc aaatatatag tgggaatgca ttattattta gctccttctt ttggctctga
     1081 caaattgaca gtccattgat atccaatgct tgcatttgca attgctgacg cacctgcccc
     1141 tgttttccct gttagtatca atggttcttt tcttgcaaga tgtgcacctg cacaaccacc
     1201 taatactaaa ccaccacct
//
LOCUS       DAWKST010000251         1219 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.251, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000251 DAWKST010000000
VERSION     DAWKST010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.251"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..429
                     /locus_tag="WJL87_002021"
     CDS             <1..429
                     /locus_tag="WJL87_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ7800408.1"
                     /translation="AIPTQTVKELDGLLQESKNIGAVNTRINIANSTTRYTPMRQTGQ
                     PVSAGFEHVLEGHFHRPIANNRSVFTISPNELKVILQSNKVVSSPVSMTPDGQYMRTV
                     DVGKVIGTTSIKEGGQPTTTIKVFTDKSGNLITTYPVKGN"
     gene            436..945
                     /locus_tag="WJL87_002022"
     CDS             436..945
                     /locus_tag="WJL87_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800409.1"
                     /translation="MSNFEKKYILELNDALSHLNHNSTSFDLLKVLISWLSNDIVIDK
                     FKILGYDFSKYIEMNPDDYPVEKSILNREEIIYLKNNIYRKISSGNFKFQYFVQYIRD
                     ILEYLFIEHIERVCPYCEWGEMQKLEEQNTHETVYLCTQCGCAFYNDNSQFLLKTPLT
                     IPMKRDEFK"
BASE COUNT          409 a          228 c          212 g          370 t
ORIGIN      
        1 gctataccaa cccagacagt taaagaactt gatggcttac tacaagaatc aaaaaatata
       61 ggtgctgtaa atacacgaat taatatagcg aatagtacta ctcgatatac accaatgaga
      121 caaacgggac aaccggtatc tgctggcttt gagcatgttc ttgaggggca cttccatagg
      181 cctattgcga ataaccgttc agtttttacc atctccccaa atgaattgaa ggttatactt
      241 caaagtaata aagtagtttc ttctcccgta tcgatgactc ctgatggcca atatatgcgg
      301 actgtcgatg taggaaaagt tattggtact acttctatta aagaaggtgg acaacccaca
      361 actacaatta aagtatttac agataagtca ggaaatttga ttactacata cccagtaaaa
      421 ggaaactaac taaatatgag taactttgaa aaaaaatata ttttagaatt aaatgatgct
      481 ttaagccatt taaatcataa ctctacctca tttgatttat tgaaagtttt gatttcatgg
      541 ttatcaaacg atattgtcat tgataaattt aaaattttag gttatgactt tagtaaatat
      601 atcgaaatga atcccgatga ctatccggtt gaaaaatcta tattgaatag agaggaaatt
      661 atttatctca aaaacaatat ttatcgtaaa atatcatcag gaaattttaa atttcaatac
      721 tttgtacaat atattagaga tattttagaa tatttattta ttgaacatat tgaaagagtc
      781 tgtccttact gcgaatgggg tgaaatgcaa aaattagaag aacaaaatac gcatgaaacg
      841 gtgtatctct gtactcaatg tggatgtgct ttttataacg ataattcaca atttttatta
      901 aaaacccctt taaccattcc aatgaaacgt gatgaattta aataaacaag ccgtagcctg
      961 catgaaccct aaaatccacg tgtagcgtgt gtgcgccagc acgcatgcgt tccatgattt
     1021 acggctcaat gccgtctgaa aagctcacaa tttttcagac ggcatttgtt atgcaagtaa
     1081 atattcagat tccctgtatg ctgtacagac gcgggagtgt taagcccccc ttgtttgaag
     1141 ctccgcggct cctgccgagc ttcaccgacc ccgttgtgcc caagctctct gctcccggcg
     1201 gctacattgt cgacatccc
//
LOCUS       DAWKST010000252         1202 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.252, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000252 DAWKST010000000
VERSION     DAWKST010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.252"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            388..543
                     /locus_tag="WJL87_002023"
     CDS             388..543
                     /locus_tag="WJL87_002023"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ7800410.1"
                     /translation="MSERKTKPSPKKGVVFPFIKKLLSINEINPNDPTIGFILLSRNN
                     PDTDYES"
     gene            complement(633..1082)
                     /locus_tag="WJL87_002024"
     CDS             complement(633..1082)
                     /locus_tag="WJL87_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ7800411.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYIEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT          400 a          262 c          178 g          362 t
ORIGIN      
        1 ttatgtctaa aggtgcaaac atgccttaaa ttaccatagt ggattaaatt taaatcagga
       61 caaggcgagg caacgctgta ctggtttaaa tttaatccac tatatattgc ataggcaaaa
      121 tacaagccta taacgaattg gaaacaaaat gccgtctgaa aacatcttca gacggcatta
      181 taaaatctgt tcaccttttc agatgagtaa tgtacaccct tatacaattt ttgctactat
      241 gccccataaa tccacggcta aagatatcct tattatgtcc tatgatttat cgaaacgact
      301 tgtaatcggc ttagcatcaa gtgccctatt cgacttatcc gaatcggata atatatttag
      361 aatggaaggg gcagaaacct ataggcaata tcagagagaa aaacaaaacc atcccctaaa
      421 aaaggcgttg tctttccatt tattaaaaaa cttctgtcaa tcaatgaaat aaacccaaac
      481 gacccaacga ttgggtttat tcttttatcc agaaacaatc cagatacaga ttacgagtca
      541 taactatagg cttaatatta cacgattttc attccatcaa ggcggaaaac cgcacaaata
      601 ctgaaacact atcgatcgat ttgtaaacaa gcctacttaa gtaacttgca gtccttatca
      661 tttcctttaa aataatccag cccgtcacta cacgaactgg cggacttctt gcaaataaag
      721 gttactagat tttcattcat cttaataata aaaggatttt tatctttatc tatggctacc
      781 gccttcaaca tgaatttact gtctaaggcc ccgcgcgcga ttccattcaa acggatacaa
      841 aagccttctg cctctttaat cggcaaactt ggccatttgg tagatgtttg tttaaatctc
      901 ccattctgca gataaaactt ttccataaaa tgtgcatttt ctaacaaggc tgcccgcact
      961 gcatttatct ttgctttctc aatataattg cgatagctcg gataaacaat taaagcaagt
     1021 acagacaata tcaagaccac tgatattaat tcaaccagcg taaacccccg attatcagtc
     1081 attactttac ttccaataag aacagattat tcaacatatt tctttgaaca gacttactat
     1141 cccattcaac agtatgcata tttcccactc tattttttag cggccggtat agccggtttg
     1201 gc
//
LOCUS       DAWKST010000253         1158 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.253, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000253 DAWKST010000000
VERSION     DAWKST010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.253"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(239..1093)
                     /locus_tag="WJL87_002025"
     CDS             complement(239..1093)
                     /locus_tag="WJL87_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ7800412.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGVGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT          326 a          345 c          301 g          186 t
ORIGIN      
        1 ctataaaatc ccgtcattcc cgcgcaggcg ggaatccgga catccaacgc tgcagcaatt
       61 tgtcggaaat aaccgaaact caaaaaaccg gatgcccgcc tgcgcgggaa tgacgatgca
      121 aagatggtcg ttatttcgga taaatatccg tcttttcgtt ccggataccc gctttcgcag
      181 gaatggagat ggaaagattt cagcctattc ggcaatcatc aaataccgct tcccaacttt
      241 atttttgatg cgacagccgg ccggcaacca aggtggcggc gatgacgaca aacacgccca
      301 aggcggacac gggcgacagg tgttcgccca aaatcaaaac cgccagcagc acgccgacga
      361 cgggttcgag cgaaatcaac agtcccgaca cgttggcagg aacacggctc atccccttgt
      421 tccacagcca ataggcgtac cagccgcacc ccacgcccaa atacagcagc gacaatacca
      481 ttccgacgct ccagtccacg gtataacttt gcgccaaagc aagcgaaaac ggcaggcaca
      541 tcaacgatgc ggcggcaatg gaaacagatg tgaatgccgg tgcgccgatg cgtgcaatca
      601 gcctttgcgt cggacgcata gcggcacaaa agcccgcgcc cgccaacaac accagcaggc
      661 agccgaacca gccgacttcg ccgccctctt ccgcaccgcc cgccatcagc agcgcgacac
      721 cggcaaatgc cgccgcgccg catatccagt ggtaggcacg cgctttgtcg ttgaagaaaa
      781 agtgtccgac aaacaccatc agcagcggct cgagtccgac aatgaccgat gcgctggcgg
      841 cggaagtgta tttcaacccg acaaactgaa gcagcagggt cagcacatag ttgacgaacg
      901 acacaatcag caacggcttc cattcctcac gcggaatctt gccgacatga cggcggcagg
      961 cgggcagtgc aggcagcgca gcaatcagca ggcgcacgcc gaccatcaat gcgggatcga
     1021 tgccgccata gacatatttg gcggcaataa acgagctgct ccagataatc agggcaagga
     1081 tttggtaaaa catagtgagt gataaagtca gatggtcata caaaagtata gcggattaac
     1141 aaaaatcagg acaaggcg
//
LOCUS       DAWKST010000254         1144 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.254, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000254 DAWKST010000000
VERSION     DAWKST010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.254"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            32..>1144
                     /gene="tuf"
                     /locus_tag="WJL87_002026"
     CDS             32..>1144
                     /gene="tuf"
                     /locus_tag="WJL87_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ7800413.1"
                     /translation="MGHVDHGKTTLTAALTTILAKKFGGAAKAYDQIDNAPEEKARGI
                     TINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREH
                     ILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSALK
                     ALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERG
                     IIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVL
                     AKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDVTGAVTLEEGVEMV
                     MPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGV"
BASE COUNT          296 a          328 c          300 g          220 t
ORIGIN      
        1 acgtagcaaa ccgcacgtaa acgttggcac catcggtcac gttgaccatg gtaaaaccac
       61 cctgactgcc gctttgacta ctattttggc taaaaaattc ggcggtgctg caaaagctta
      121 cgaccaaatc gacaacgcac ccgaagaaaa agcacgcggt attaccatta acacctcgca
      181 cgtggaatac gaaaccgaaa cccgccacta cgcacacgta gactgcccgg ggcacgccga
      241 ctacgttaaa aacatgatta ccggcgccgc acaaatggac ggtgcaatcc tggtatgttc
      301 cgcagccgac ggccctatgc cgcaaacccg cgaacacatc ctgctggccc gccaagtagg
      361 cgtaccttac atcatcgtgt tcatgaacaa atgcgacatg gtcgacgatg ccgagctgtt
      421 ggaactggtt gaaatggaaa tccgcgacct gctgtccagc tacgacttcc ccggcgacga
      481 ctgcccgatt gtacaaggtt ccgcactgaa agccttggaa ggcgatgccg cttacgaaga
      541 aaaaatcttc gaactggctg ccgcattgga cagctacatc ccgactcccg agcgtgccgt
      601 ggacaaaccg ttcctgctgc ctatcgaaga cgtgttctcc atttccggcc gcggtacagt
      661 agtaaccggc cgtgtagagc gcggtatcat ccacgttggt gacgagatcg aaatcgtcgg
      721 tctgaaagaa acccaaaaaa ccacctgtac cggcgttgaa atgttccgca aactgctgga
      781 cgaaggtcag gcgggcgaca acgtaggcgt attgctgcgc ggtaccaaac gtgaagacgt
      841 agagcgtggt caggtattgg ccaaaccggg tactatcact cctcacacca agttcaaagc
      901 agaagtgtac gtactgagca aagaagaggg cggccgccat accccgttct tcgccaacta
      961 ccgtccccaa ttctacttcc gtaccaccga cgtaaccggc gcggttactt tggaagaagg
     1021 tgtggaaatg gtaatgccgg gtgagaacgt aaccattacc gtagaactga ttgcgcctat
     1081 cgctatggaa gaaggcctgc gctttgcgat tcgcgaaggc ggccgtaccg tgggtgccgg
     1141 cgtg
//
LOCUS       DAWKST010000255         1142 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.255, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000255 DAWKST010000000
VERSION     DAWKST010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.255"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..570)
                     /gene="rfbA"
                     /locus_tag="WJL87_002027"
     CDS             complement(<1..570)
                     /gene="rfbA"
                     /locus_tag="WJL87_002027"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ7800414.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQIKPS"
     gene            complement(633..>1142)
                     /locus_tag="WJL87_002028"
     CDS             complement(633..>1142)
                     /locus_tag="WJL87_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEZ7800415.1"
                     /translation="CSNNYGPYHFPEKLIPLMILNALDGKPLPVYGDGMQIRDWLFVE
                     DHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTICALLEELAPEKPAGVARYEDLI
                     TFVQDRPGHDVRYAVDAAKIRRDLGWLPLETFESGLRKTVQWYLDNKTWWQNVLNGSY
                     RLERLGTGK"
BASE COUNT          254 a          284 c          313 g          291 t
ORIGIN      
        1 ggacggtttg atttgtttgg cgaactcgac ggcacggttg tcgtagaagt acaagccggt
       61 taccgcccaa tcggatttgg gccgttgcgg tttttcttcg atggaaacgg cgcggaagtt
      121 ttcgttaaat tcaaccacgc cgaaacgttc ggggtttttg acctgataag caaacacggt
      181 tgcgccgtgc gtttgcgctg ccgcctgttt caatgtttgc gtaaacgact gaccgtaaaa
      241 aatattgtcg cccaaaacca agcaaacatt gtcgttgccg ataaattctt cgccgatgat
      301 aaatgcctgt gccaagccgt ccggactggg ttgcacggca taactgatgg aaatgccgaa
      361 atcgctgccg tcgccaagca ggcgtttgaa agaggcgttg tcttcaggcg cggtaatcac
      421 caaaatatcg cggattcccg ccagcatcaa aaccgacaag gggtaataaa tcatcggttt
      481 gtcgtacacg ggcaggagct gtttggatac gccgcgcgtg atggggtaga ggcgcgtgcc
      541 gctgccgcct gccagtatga tgcctttcat ctttgttttc ttcctttgcg atgggttttc
      601 agacggcatt gcgtcgggat gccgtctgaa aactattttc cagtacctaa acgttccaaa
      661 cgatagctgc cgttcaatac attttgccac caggttttgt tgtccagata ccattgcacg
      721 gttttgcgga ggccggactc gaaggtttcc aaaggcagcc agcccaaatc ccgcctgatt
      781 ttggctgcgt cgacggcgta gcgtacgtca tggccggggc ggtcttgtac gaaagtaatc
      841 aaatcttcat aacgcgccac accggccggt ttttcgggag cgagttcttc cagcagggcg
      901 cagatggttt tgacgacttc aatattggct ttttcattgt ggccgccgat attgtaggtt
      961 tcgccgacaa caccttcggt aacaacctga tacagtgcgc gcgcgtggtc ttcgacaaac
     1021 agccagtcgc ggatttgcat accgtcgccg tacacaggca gcggtttgcc gtcaagcgcg
     1081 ttcagaatca tcaaaggaat gagtttttcc ggaaaatggt aaggaccgta gttgttggag
     1141 ca
//
LOCUS       DAWKST010000256         1110 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.256, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000256 DAWKST010000000
VERSION     DAWKST010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.256"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(172..609)
                     /gene="rnhA"
                     /locus_tag="WJL87_002029"
     CDS             complement(172..609)
                     /gene="rnhA"
                     /locus_tag="WJL87_002029"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ7800416.1"
                     /translation="MDTPVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDTLVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            722..>1110
                     /locus_tag="WJL87_002030"
     CDS             722..>1110
                     /locus_tag="WJL87_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HEZ7800417.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKAENLPEVLLSGYSSEEGEWVCL
                     NVLQGDVEVRTQDGAAEVWSAESGDCVFAPQQVFSVKLKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAARDTVPAGRALDMG"
BASE COUNT          199 a          258 c          359 g          294 t
ORIGIN      
        1 ctggattccc gcctgcgcgg gaatgacggt ggagcggttt cagttgtttc taaataaatt
       61 tttgcagcgt tgggttttta gattcccgcc tgcgcgggaa tgacggtgga ggacgatgcc
      121 gtctgaatcc cattagcggt ttcagacggc attttcttgt cggtgcgaca gtcaggaaaa
      181 ctgcgctgcg ccacggtttg ccaaatcgtc ggcgcgttcg ttttccgcgt gtcccgcgtg
      241 tcctttaacc caagtccaac tgacttggtg ctgcccgacc agagtgtcga gttctttcca
      301 taaatcgtcg tttttgacgg gctgtttggc ggcggttttc cagccgttgc gcttccaacc
      361 gtgtatccag ttttccatgc cgtttttgac gtattgcgag tcggtgcaga tgatgacggt
      421 gcagcggcgt ttgagcgatt tcagtccttc gatgacggcg gtcagctcca tgcggttgtt
      481 ggtggtttgc gcttcgccgc cgaaaagttc tttttcgtgg ctgccgtagc gcatcaacac
      541 gccccagccg cccgcgccgg gattgccttt gcacgcgccg tcggtgtaaa ggtaaacggg
      601 tgtgtccata ttgtgccttt gtgtggtgtc cggcgtgatt ataagcggtt tgcgcgccgt
      661 gccgtctgaa agattgcggc ggattgaaat aggcatataa tgtgcaactt tatttttgaa
      721 tatgaaagag cggatagtgg gtcaaagcgg cgagttgttt tgtttcgggc aaatgcccgt
      781 atggaaggcg gaaaacctgc cggaagtttt gttgtcgggc tattcgtccg aggaagggga
      841 atgggtctgc ctgaatgtgt tgcagggcga tgtcgaagtc cgcacgcagg acggggcggc
      901 ggaggtttgg tcggcagaaa gcggcgattg cgtgtttgcg ccgcagcagg tgttttcggt
      961 caaactgaaa acggacgatg ccgaaatccg tttgtcgctg tattgcgcgg cggcagacta
     1021 ttttcacaaa aaatacggga tgagtgccac gcattctgcg gtcgcggcgg cacgggatac
     1081 cgtaccggcg ggcagggcgt tggatatggg
//
LOCUS       DAWKST010000257         1100 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.257, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000257 DAWKST010000000
VERSION     DAWKST010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.257"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            430..>1100
                     /locus_tag="WJL87_002031"
     CDS             430..>1100
                     /locus_tag="WJL87_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ7800418.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCITARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLP"
BASE COUNT          329 a          263 c          235 g          273 t
ORIGIN      
        1 tttctgtttt tgattttctg ttttcgtagg aatgacggtt taaaagttac ccgaaacctc
       61 aaaaaaaagg ctgtgtttta atatagtgtt gatagacgta cttggcttcc atgtattcaa
      121 tcgtggaaat ctatatcttc gtcctcgccg aaatagtcta tgcccgatat acaattttga
      181 tacacaaact tggaaatatg ggtatcgtcg ccggagcgat agaatgcgga cagttatata
      241 tatacggttt ttttagggaa gcgggcagat gaagggcgga ttttgaatta accccatgca
      301 aattgacttt tgcaggcggt tttacctccc atatatttac aaaagccaaa tttttaaaca
      361 tatatccttg atatatacac ggcgtaaaca tatactggaa acatctttaa attttccgaa
      421 attttaaata tgagcaactg gaaacccgat attccctata acgatttacc acccctgccg
      481 ccaaaacagg atattgaaag caaaaccatc ctgaaacgtt gtataaccgc ccgtgcatcc
      541 cttgcccgtt taaagcaggc ggcagaactg ataccgaatc aagccatgct gattaacacc
      601 cttcctgtta tggaagcccg tgcaagttcg gaaattgaaa acatcgtaac cacaacggat
      661 aagctgtttc aatccctgca aatggatacg gaacggcaag accctgccac gaaagaagcc
      721 ctgcaatacc gcaccgccct gtttgcaggc tatgaatcac tgacgagccg ccctttatgc
      781 acacaaaccg ccatcatggt ctgcaacgcc atcaagcacc cctacgaaat ggccatccgc
      841 aaaacaggcg gcacagccct aaaaggaggc aacagcggaa atgttgtcta taccccgccc
      901 gaaggagaag aaaccatacg cggcaagctg gcaaattggg agcggtttat tcacgaaagc
      961 ggcgatttag acccgcttat catcatggcg gcggcacatt accaatttga agccatccat
     1021 ccgtttacgg acggcaacgg gcggacgggg cgcatattga acagcctgct attgattgaa
     1081 aaagggcttt tggatttgcc
//
LOCUS       DAWKST010000258         1070 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.258, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000258 DAWKST010000000
VERSION     DAWKST010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.258"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(122..550)
                     /locus_tag="WJL87_002032"
     CDS             complement(122..550)
                     /locus_tag="WJL87_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ7800419.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(531..965)
                     /locus_tag="WJL87_002033"
     CDS             complement(531..965)
                     /locus_tag="WJL87_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ7800420.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTDKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
BASE COUNT          278 a          318 c          191 g          283 t
ORIGIN      
        1 tttgaaaacc ccattaaacc gtctttaacc catcgtcccg aacggtagat gtttcacccc
       61 tgcaaaccaa aaaaaagacc gtctgaatac agacggccaa agcctggtca cacatcactg
      121 cctaaaataa agctgcctgc tgtgccgcag gtcggctcat ttcattgata atcgtatatc
      181 ccgttcgtga agagatgccg tatttagggc atagcttcgt catcgccata agcccgctct
      241 ccttatcaat atcgcgcaat ttgacaaact cctgataaaa cctatggttt ctcaactgta
      301 ttaacgcctt gccgcaccgt gggacataca attcctcgcc accatatacc tgcaacagct
      361 catgtgtttt cacttcgccg atggcttcga ccaaaattgc caaacgctcg gtgtccacct
      421 tgcccttacc aaatttaaac cgcgccccgc caatcgcctt gaccagctgt tccgtcgctg
      481 ccagtccgat gacatccaca atgtccaaca cggtatccgg caataaatgt tcaacttttt
      541 cgaaccccat catccccacc cgctttttcc gttttcctgt tttccgcaat ctgcaacgca
      601 gcaaccagtt tatgtagctg cgtatcgtct aaatattcga ccttatcctt accaaacatc
      661 cgccgcgcca ttgcgtgtgc atagttccaa tgtttgccgc cgacggtcag cagggcttcg
      721 actttgtcca gcattgccgc tgatgatgtc cgacgcagat gcggtttgcc gtgtgggtta
      781 ccttttgctt taggcttaaa tccgtgcgac cgcatatcag cgacaacaga ctcaagttca
      841 gaaacatcca tatccgcaca cgaccgctta tccgtcacac gctccaacac cgcgcgatag
      901 gtaccgtcat ccaagcccag ctccttttga gcaatcttaa ttttcgcaat caacgcccgg
      961 cgcattatct ctctaataca acatatagta taaattagcg cattttatac aataaaatac
     1021 aatatatatt attaagccga tgtttttttg cgaaacggac agacataaaa
//
LOCUS       DAWKST010000259         1032 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.259, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000259 DAWKST010000000
VERSION     DAWKST010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.259"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(299..685)
                     /locus_tag="WJL87_002034"
     CDS             complement(299..685)
                     /locus_tag="WJL87_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEZ7800421.1"
                     /translation="MRKILLDTHALLWWLLDDKKLGTSARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(682..927)
                     /locus_tag="WJL87_002035"
     CDS             complement(682..927)
                     /locus_tag="WJL87_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEZ7800422.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLTGIEKP
                     ARNAGRLKKFSHMGNTDISRILEDDNETAALFEESAL"
BASE COUNT          278 a          243 c          185 g          326 t
ORIGIN      
        1 cagccatata tattaagtac ctgctcttta tgcgcttttt taggaaagga agatcaaacg
       61 gttcaatagc gaagtcttgc ccaaatttga tggacaggat atagaataca tgcgtgcata
      121 ccacaccctg ccaaagttgg gagatgcact taaggttgca cttgtggtcg ccaactatgc
      181 ctatacacgc ggatttcccg cgtttatagg ccgcgacgga agtctggctt ttaagcgaag
      241 acaataaccg aaaagaccgc ctcggcggtc tttcttgtgt acacagtttt tgtatttgtc
      301 agcttgatgc attgacaact ctaattccat attgcggaat atattcatcg acagtcatca
      361 gttcaaagcc ttccgcttgg gtttgtgcaa tcaacatcct atcgaaaggg tctttgtgta
      421 tttccggaag gcttccagcc tgttttgcat gaaacagacc tataggcagc atttcaaaat
      481 cctcttcttg aagcacatca aaaaactctt ccggtaattt caacaacccc ttgttctgct
      541 tgatggaaat ttcccaaata cttgctgcac tgacaaagat cgcatttctc ggattttcta
      601 tcagtttgcg tgcagatgtc cccagtttct tgtcatccaa caaccaccac agcaaagcat
      661 gggtatcaag cagaatcttt ctcacagagc cgactcctca aataaagctg ccgtttcatt
      721 gtcatcctca agaatacgtg aaatatccgt atttcccata tgactgaatt ttttcaacct
      781 tcctgcattt cgtgccggtt tttcaatacc ggttagttgg acgcaaggct tacctgcttt
      841 cgcaataata acgatttccc ctgcttctgc tctttgaatc aattgactca aattggtttt
      901 tgcctgatga atatttgctt gaaacataac acttctcatg attagctaac ttaactaata
      961 tacatcatta ccaagatttt gggaatctca ttacatatat ttgattatat ccgccgtttt
     1021 attcacacct tg
//
LOCUS       DAWKST010000260         1019 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.260, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000260 DAWKST010000000
VERSION     DAWKST010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.260"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..100
                     /locus_tag="WJL87_002036"
     CDS             <1..100
                     /locus_tag="WJL87_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ7800423.1"
                     /translation="IGRDKLFIHPRLMELLRGEGNSFTSFQSLVKA"
     gene            130..1008
                     /locus_tag="WJL87_002037"
     CDS             130..1008
                     /locus_tag="WJL87_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ7800424.1"
                     /translation="MARRSKTFEEAAAEVEERFGHRGIKLVEFEGTAKPCVINCPKHG
                     NQTCSRYSNMFIGSSWGCPSCGNEQAAKAGIATLRKNHIALEMLKQAVTGMTKQERIT
                     TQAYNEMTKSVAGSNSIVLNDVQGDTTINNHHTHTHNHSDADGKALSMRLTPRPLLSD
                     RQAAAFARTGKLTGSFDLFASVVAPSQYTFAVAMPDTSMSPVIEKGDLLVVEPRMRPA
                     DEDIVLIELSDKRLVVAHLVIDIAGRMLIYQTGRPSEALDLPEGSVILGVVLEVKKRF
                     MSAAQARRRVDSDYRP"
BASE COUNT          264 a          260 c          276 g          219 t
ORIGIN      
        1 catcggcagg gacaaactat tcattcatcc gcgcctaatg gaactattgc ggggagaggg
       61 caacagcttt acctcattcc aatccctcgt taaagcatag ccaaaataat caataatccg
      121 gaggtcaata tggcaagaag gtcaaaaaca tttgaagaag ctgctgctga ggttgaggaa
      181 cgtttcggtc atcgtggcat taagttggtc gagtttgagg gtacagccaa gccgtgtgta
      241 atcaactgcc ctaaacatgg aaaccaaacc tgttcgaggt actccaatat gttcatagga
      301 agtagctggg gttgcccctc ttgtggtaat gagcaagctg caaaagccgg tatagcgacc
      361 cttaggaaga atcacatagc gttagaaatg ctgaaacagg ctgtaacagg tatgaccaag
      421 caagagcgca tcacgacgca agcctacaat gagatgacca aatccgtggc aggttcaaac
      481 agcatagtcc ttaacgatgt ccaaggcgat acgaccatca acaaccatca tacgcatacg
      541 cacaaccaca gcgatgccga cggcaaagca ctgtcgatga ggctcacacc acgccccctg
      601 ttgtcagacc gtcaggcggc ggctttcgcc cgtacaggca aactcacagg cagtttcgac
      661 ttgtttgctt cggtggtcgc cccctcgcag tacacgtttg ccgttgccat gcccgacacg
      721 tccatgtcgc cggttatcga aaagggggat ttgctggtgg tcgagccgcg tatgcgccct
      781 gcggacgaag acatcgtact gattgaactg tccgacaagc ggctggtcgt cgcgcacctt
      841 gttatcgata ttgcgggcag gatgctgatt tatcagacgg gcaggccgtc tgaagccctc
      901 gacctgcccg aaggcagcgt gattttaggt gtggtgctgg aggtcaaaaa acggtttatg
      961 tccgccgcac aggcaagaag gcgtgttgat tcggattacc gcccctgatg tgtggacgg
//
LOCUS       DAWKST010000261         1006 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.261, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000261 DAWKST010000000
VERSION     DAWKST010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.261"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..>1006
                     /locus_tag="WJL87_002038"
     CDS             <1..>1006
                     /locus_tag="WJL87_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-regulated protein frpC"
                     /protein_id="HEZ7800425.1"
                     /translation="IERSYYKAVTDNGVSSSAAIDLVINRSLPDMADGYWALGLGIEA
                     ERIHNEQAVNNPNGSERDNRKQLISALDKGFDGSFKEKHFTFLQSVMMDLTKLGVEYT
                     IDGWQKIGGWGNGIINDLYKSVVKREWTGIFEIVNNNIKQGNEAFKNEINSLVHDMKA
                     AGKEFGDDLNTQWNNLTQAAEIIYNDIVDNTSQGIEKGVKAIKELSEKMKNAASDLAD
                     GSAEKAKQVVEDLAQAAKEAYENAKSTAEKAAQAAREFFKGLPSFKDLAEKFRDLFPN
                     PEGWIDDGHQCLAPWVKETKKRNGKYHVYDPLALDLDGDGIETIATKGFAGALFDHRN
                     "
BASE COUNT          350 a          161 c          232 g          263 t
ORIGIN      
        1 aaattgagag aagttactat aaagccgtta ccgataatgg cgtttcttcc agtgcagcta
       61 ttgatttagt tattaatcgt tcacttccgg atatggcgga tggttattgg gcattaggtt
      121 tggggataga agccgaacgt atccacaatg agcaagcagt aaataatccg aacggtagcg
      181 aaagggataa tagaaagcag ttaatatctg ctttagataa aggatttgat ggatctttta
      241 aagagaagca ttttactttt ttacaatctg tgatgatgga tctaacaaag ttaggtgttg
      301 aatatacaat agatggttgg caaaaaattg gaggttgggg taatgggata atcaatgatt
      361 tatataaaag tgttgtaaaa agagagtgga ctggaatatt tgagatcgtt aataataaca
      421 tcaagcaagg aaatgaagct tttaaaaatg aaatcaatag cttggttcat gatatgaaag
      481 ctgctggcaa ggaatttgga gatgacttaa atacacagtg gaataatctc actcaggctg
      541 ccgaaataat ctataatgac atagtagaca atactagtca aggaatagaa aaaggtgtca
      601 aagccattaa agaattgtct gaaaaaatga aaaatgctgc ttccgatttg gctgacggtt
      661 cagcagagaa agctaaacaa gtagtggaag atttggctca agccgccaaa gaagcatacg
      721 aaaatgccaa atccacagcc gagaaggctg ctcaagcagc tcgagaattt tttaagggct
      781 tgcccagttt taaagatctg gccgaaaaat ttagagatct gttcccaaat ccggaaggct
      841 ggatcgatga tggtcaccaa tgtttagctc cttgggttaa agaaactaaa aaacgcaatg
      901 gcaaatatca tgtctacgac ccccttgccc tagacctaga tggcgacggt atagaaacca
      961 ttgccaccaa aggctttgca ggtgcattgt tcgaccaccg caatca
//
LOCUS       DAWKST010000262          905 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.262, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000262 DAWKST010000000
VERSION     DAWKST010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.262"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..215
                     /locus_tag="WJL87_002039"
     CDS             <1..215
                     /locus_tag="WJL87_002039"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800426.1"
                     /translation="DAGDVYCVYNIRLKQYTACQVTRVEEHGGKKTHATLLALDWQGN
                     KPLGAEELADLKPLYKDLKNNIGNIV"
     gene            546..>905
                     /locus_tag="WJL87_002040"
     CDS             546..>905
                     /locus_tag="WJL87_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800427.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDG
                     YDVYRQLRWQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLY"
BASE COUNT          282 a          147 c          211 g          265 t
ORIGIN      
        1 ctgatgccgg agacgtatat tgcgtctata acatcagact gaagcagtac actgcctgcc
       61 aggttacccg agttgaagaa cacggtggca aaaaaacaca tgcgaccctg ctggctttgg
      121 actggcaggg caacaaaccg cttggggcgg aggagctggc ggatttgaaa ccactttaca
      181 aagacttaaa gaataatatt ggaaatattg tatgaacaaa aaattaaact atatttttat
      241 gttggactgt ttagggttgg tgatattgtt tacttgtata atagctactt ttgaaagaga
      301 ttatggattt aaaattttta ctaattctaa gaggcctgaa ttttattatt ggattggaat
      361 gttttattat ggaattattt cttgctggtt tgattatcaa ttaatttcaa caaaggcgaa
      421 ttcgtataaa agaaaagtta aacaatataa aattttttca gtaatatttt cagttttgat
      481 atttatttct actatagtaa aactttaaat tttggagcaa aaatttatga gcgattcaat
      541 tgaatatgta ttgggaacgc ggtctgcaca tgtataaggc aagtgctgtc gtgccaacgg
      601 gatatgtacg ggttgggaat accgcgccgc tggtcggcga agacacgcaa cggtatgcct
      661 ctttttgggg cgacggctac gacgtgtacc gtcagttgag atggcagcag atacccgaaa
      721 aacagagaaa ggcattcaaa aaagccgcca aaagcaaaaa gaccgtgatg tttgccggac
      781 gggaatacgg catatccaaa cagaatttga gcgatgtttg ggatgatttt gaagacgcga
      841 tggaactgaa ggcgtttccc tgcctgtctt cgctgtttct gaccaagtgg cataaaaatc
      901 tatat
//
LOCUS       DAWKST010000263          904 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.263, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000263 DAWKST010000000
VERSION     DAWKST010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.263"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(87..719)
                     /gene="mtrR"
                     /locus_tag="WJL87_002041"
     CDS             complement(87..719)
                     /gene="mtrR"
                     /locus_tag="WJL87_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ7800428.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT          200 a          201 c          238 g          265 t
ORIGIN      
        1 tgtattttta gaagaaggct ttgtgtcatt ttgatgccgt ctgaaagggt ttgttcgttt
       61 cagacggcat tgttaccaag gcttgattat ttccggcgca ggtcgggatg gttttccaag
      121 ttgtccatca ttatcccgat gatgcgcggg gcggttttgc ccaaatcgaa acgttcgcag
      181 gaagagaacc accgccaaat cagcccgtcc aaggttgatt tgatgaagat gaccgctgtt
      241 tccttgtcca aatcgtcagc caaatcctga ttttccaccg cttcggtcaa aacggcggta
      301 attttctcgc gccagattgc ctgatgcttg cgggcaatgg cgataacggc ggcgttttgc
      361 tccgtgtgtt cgcattttaa aaacaggatg ttgtggaatt tgtagtagat gtcgttgctt
      421 tgcagccgct cgaaaaagtg cagcagcgtg tggcggaata ccgcccaaga ccctccttcg
      481 gcatcttcgg catcttgcgc gatgcagttt tcgatgtcgt cgcagatacg ttggaacagc
      541 gcgtcgaaca agtcttcctt atttttgaaa tgccaataga gcgcgccgcg cgttacgccg
      601 gcggcttggg cgatttcgtt gagcgaggtg cgcgcaatcc ctttgcggta aaaggtttcc
      661 aaggcggcaa gcatcaggtg ttctttggtt ttcaaggctt cggttttggt ttttctcata
      721 atggcgtttt cgtttcgggt cggtttgatg agggcggatt ataaaaaaga ctttgtaacc
      781 atgcaatcgt gtatgtataa tgaaacccat gaaattgaga ctacatctca actttgaaaa
      841 caggcataac ctgctttgca acccgttcga acattcgggt atcaaagcag gcggttttta
      901 tatc
//
LOCUS       DAWKST010000264          888 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.264, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000264 DAWKST010000000
VERSION     DAWKST010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.264"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(93..644)
                     /locus_tag="WJL87_002042"
     CDS             complement(93..644)
                     /locus_tag="WJL87_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800429.1"
                     /translation="MKVLNGWSDRKMWRVLNALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDLEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFQ
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
     gene            complement(698..888)
                     /locus_tag="WJL87_002043"
                     /pseudo
     CDS             complement(698..888)
                     /locus_tag="WJL87_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          249 a          307 c          164 g          168 t
ORIGIN      
        1 agcaaagaaa ccgccattag cactacaccc ccccaagctg cggctgccaa acacatcggc
       61 tgcttctcga cgcacccgct gaaaaatgcg tcctatccgt ccgccttgcc cgcaccccct
      121 gactttgccg ccctgcccga tgccgtctga agcctgaacc gccccaccag cccgatgacg
      181 cacgacaccg ccggaaaggg catacacaaa gccgtcagca cataacgcgc attccccgta
      241 ttgaccacgt ccgcgccctt cagcagcagc ggtagcgcgt tcgcccactc ccacaccgac
      301 ggcgcgctga acgccaatac cgccaccagc cccaacatct ggaacacccc gatttccaac
      361 acctgccgcc gccgcaccga acggttgagc aaaaacacaa ccgccaaccc gaaacagacc
      421 accgccgcca taccgccgtt ggcgacaatc tgcaaactgt tgaatgcaat atcgggcgta
      481 accgccaaca cgccttccaa atccggcacg gcacgctgca aatccaaacc ctgcaacaca
      541 ttcaacacaa aaccgtagat caaatcaaaa aacaccacgc ctatcggcaa agcattcaaa
      601 acccgccaca tcttcctgtc cgaccaaccg ttcaaaactt tcattttacc cgaccacgca
      661 agccgccgaa caaaaaataa ggggctgtcc tagataacta ggacaaactt gattttacta
      721 attgttttaa aatggaacaa gaacttttat ctcactgttg ttaaaacgtc gttcgcactc
      781 ctttaaatac agctcaaaat gcgctttggg aatgccgtta aacttgcgta aatgacgttt
      841 tgcctggttc caaaagttct caattccatt aatatggttt tgtcgttc
//
LOCUS       DAWKST010000265          867 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.265, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000265 DAWKST010000000
VERSION     DAWKST010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.265"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(48..827)
                     /locus_tag="WJL87_002044"
     CDS             complement(48..827)
                     /locus_tag="WJL87_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ7800430.1"
                     /translation="MKLVRPQKISDQVLSVLEGRIVEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
BASE COUNT          200 a          288 c          173 g          206 t
ORIGIN      
        1 ttcactatac cgatacatcc gaatatgcca gaccgtctaa acggtattca tacctcccaa
       61 tcctgcacgc gcttcaaatc gttttgcgcc aaagtatccg cgtggcggtt acggctctga
      121 tattccctgt ctttcatgat gctgcttgcc acataattca aatgtgcctt tgccgcctcc
      181 gaagcctcgc ccggccgacg gttcgatatc gcctcataca gcacgcggtg ctgcgccatc
      241 agtttcggac gcggatcttc ttcctgattc agataaataa ggctgctgcg cgtctgccgg
      301 tacagcattt tcaacaaacc gcccgacaaa tggctgaaca acaaattgtg cgccgcatcc
      361 gcaatcgtct gatggaagct gacatcggct tcgctctgat gttccagatt gccgctttcg
      421 cacgcctcct caaacttttc aagccaaaac ccaatccgct tcaaatcggc atccgtccgg
      481 cgttctgccg ccaatgccgc catacagccc tcgatgtggc agctgaaatc aaaaacatcc
      541 tgttcccaat tggaatgctt gcccaaaagc tcctgccaac tttgcaaaaa atcctgctgc
      601 ggcttgaccg aaacataata accgtctccc tgcctcgctt ccaaaacctg acgggcgacc
      661 aaaacattca atgccgacct gaccgacggg cgggacacgc cgaactcttc cgccaaaacg
      721 cgttcgggcg gaatcttgcc cccttccgcg taaacccctt ccacgatgcg gccttccaat
      781 accgacaata cctgatcgct gattttctga ggccttacca gtttcatcac tcctccttta
      841 taaagattcc ctgccgaacc cttccga
//
LOCUS       DAWKST010000266          861 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.266, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000266 DAWKST010000000
VERSION     DAWKST010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.266"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(5..625)
                     /locus_tag="WJL87_002045"
     CDS             complement(5..625)
                     /locus_tag="WJL87_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ7800431.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
BASE COUNT          292 a          187 c          196 g          186 t
ORIGIN      
        1 cggtttagtg ttcactatat ccatcctttc tgtaacgcag ccatactgaa gcagcaatca
       61 gaaggttaaa gcaggctgtc agcgcaatgg tttgggagag ggtaaaaaag acgtagaaaa
      121 attcggcggc ggcaagcgat ccgagtgccg cacccaaagt gttgaaaaaa tataaggtac
      181 cgatagactc gccaacatta tgtattttcc ggttaaaaaa acaggtcagc aagggcaagg
      241 tcgcgcccat cataaaggta ggaagcagca ataagaggaa attggcagca gcgatgatgg
      301 gcaaatcagc ctcaactaaa agatgcccca agccggaaat cagacccctg cttaccaaac
      361 cgaacagacc gatggatact tcagcgatgc aaaacagggg gatgatactt gaaggaaaac
      421 ggtcagcaat gcgtccaccg aaatacgcac ctacacccaa gccgaccata aatacagaaa
      481 taatgacagt aatcgaactc aaatcgatac ctatgtgact gaatagaagc ctctgccagc
      541 tgacctggta aatcagggcg cagaatccag aggcgaaaaa caccagcgac aatcctttca
      601 gggttttact tgcggtcgaa ttcatatttt ctattttcaa ataggcggaa ctataaatga
      661 aatgtatcaa tgtgtaaaca tttgtattaa ccgtaggaaa tcaccgtatt gctcaaactg
      721 caggccggga aaataaactc gtaagcccgc aaacggcgta tcactgttca aactgctgtg
      781 cggcttcacg gtgttataaa aataatgggg ctgtcctgga caactaagat aaactcgatt
      841 ttactaattg ttttaaaatg g
//
LOCUS       DAWKST010000267          840 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.267, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000267 DAWKST010000000
VERSION     DAWKST010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.267"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            114..>840
                     /locus_tag="WJL87_002046"
     CDS             114..>840
                     /locus_tag="WJL87_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ7800432.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYRIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDSRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAID"
BASE COUNT          241 a          199 c          182 g          218 t
ORIGIN      
        1 ggtttttgtt aatccactat aatacgcagg caaacccaag ccttgctggg ttacaggatg
       61 aaaccctgcc gtagcagacc gcatttccaa tccatattca aggtgtccgc cttatgaaaa
      121 tcgtttttat cacaacagtc gcatccagca tttacggttt ccgcgccccc gtcattaaaa
      181 aattaatcgg caaaaaccat caggtgtatg cctttgtatc ggagttttcc gataatgaat
      241 tggatattat cagggaaatg ggggttacac ccgttaccta ccgttcaaac cgcagcgggc
      301 tgaacccgtt ttccgatata aaatccacct tcctcatctt taaagaactc aaaaaaatat
      361 cgccggattt ggttttccct tatttcgcaa aacccgtgat tttcggcact tttgccgcaa
      421 aactggcagg cgtgcccaga atcgtcggga tgctggaagg tttgggattc gcatttaccc
      481 cgcagccgga aggcataccg ttaaaaacaa aaatcataaa ggggatcttg attgccttat
      541 accgcattgc cctgccgatg ttggaaagcc tgattgtatt aaaccccgac gacaaagacg
      601 aactgacgga caaatacggc atcaaaataa aaaacatcca tattttgggc ggaatcggtc
      661 tggattcgcg gcaatatcct tattccgagg cggatattcc cgatgaaaaa gaacccgtaa
      721 aattcctctt tatcggcaga tttctgaaag aaaaggggat tgatgatttt attcgggcgg
      781 cggaacaggt taaggacaaa taccccgata cggtttttac cgctttgggc gcaatcgaca
//
LOCUS       DAWKST010000268          805 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.268, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000268 DAWKST010000000
VERSION     DAWKST010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.268"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..743)
                     /locus_tag="WJL87_002047"
     CDS             complement(join(<1..524,524..743))
                     /locus_tag="WJL87_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242698.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ7800433.1"
                     /translation="MARTAITDNIWEQLQTTMKAHGCHQWKNDRTVMEAILWKLRTGA
                     PWRDIPIELGSWKTAYNRFNRWSKKGLWQKFFFDLRKEIDKEWVFIDGSYVRCHQHAS
                     GARRGFDRAIGQSRGGNTTKIHLCVDSHGNPLDFKVTGGNVHDGQVANDLIEVIQEAQ
                     YFIADKGYDSQEIRDKAIEHGMKAIIPKRKNAKKPNPDFDSYLYKLRHLVENAFARLK
                     QFRSIATRYEKLARNFKSMLYLACSIIHAK"
BASE COUNT          193 a          175 c          160 g          277 t
ORIGIN      
        1 cttagcatga ataatagagc aagccaagta aagcatcgat ttaaaattac gagctaattt
       61 ctcatagcga gtagcaatac tacgaaactg ctttaatcgt gcaaatgcat tctcgactaa
      121 gtggcgtaat ttataaaggt agctatcaaa atcagggtta ggtttcttgg catttttacg
      181 ctttggtata atagctttca taccgtgttc tatcgcttta tctctgattt cttgcgagtc
      241 ataccctttg tcagcgataa aatactgggc ttcttgtatg acttctatca agtcgtttgc
      301 aacttgaccg tcgtgcacgt tacccccagt gactttaaaa tcgagcggat ttccatgcga
      361 gtccacacat aggtgtattt ttgtcgtgtt tccgccacgg ctttgtccaa ttgctctatc
      421 gaaaccacgc cgagctccac ttgcatgttg atgacaccgt acataacttc cgtcgatgaa
      481 tacccattct ttgtcaattt cttttcgtag atcaaaaaaa aattctgcca caagcctttt
      541 ttagaccatc ggttaaagcg gttataagcg gttttccatg accctagctc aataggtatg
      601 tctcgccatg gtgcacctgt tcttagcttc catagtatgg cttccatcac ggtacggtcg
      661 ttcttccatt gatgacagcc gtgcgctttc atggttgttt gtaattgttc ccatatgttg
      721 tcagttattg cggttctcgc cattgtttgt gtttgcctat atttcgttgg aatatagggt
      781 taaaataggg ctcttttggg cttat
//
LOCUS       DAWKST010000269          773 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.269, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000269 DAWKST010000000
VERSION     DAWKST010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.269"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            89..607
                     /locus_tag="WJL87_002048"
     CDS             89..607
                     /locus_tag="WJL87_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800434.1"
                     /translation="MKQMLLAVGVVAVLAGCGKDAGGYEGYWREKSDKKEGVIAVKKE
                     KGNYFLNKINVFTGKEESLLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTAQAYLDARKALPSNQTYQQRQAAIEQLKRRFEAEFDELEK
                     EIKCNGKPTLLL"
BASE COUNT          228 a          157 c          221 g          167 t
ORIGIN      
        1 caactatagt ttttgcaaat caatattgac aagctgaaaa aaaaaaaaca atatactcgc
       61 tcggtcttaa tgttaacgga gtatggaaat gaaacaaatg cttttagccg tcggcgtggt
      121 ggcggtgttg gcgggctgcg gcaaggatgc cggcggttac gagggttatt ggcgcgaaaa
      181 gtcggacaaa aaagagggcg tgattgccgt caaaaaagaa aaaggcaatt acttccttaa
      241 taaaatcaac gtatttacag gcaaggagga gtctttgctt ttgtctgaaa aagacggcgc
      301 gctttcgata aacacgggga taggggaaat cccgatcaaa ctttccgacg acgggaaaga
      361 gctgtatgtc gaacgcaggc agtatgtcaa aaccgatgcg gcgatgaagg acaaaatcat
      421 cgcccatcag aaaaagtgcg gacaaacggc acaggcatac ctcgacgcgc gaaaggcgtt
      481 gccgtcaaac caaacgtatc agcagcgtca ggcggcgatc gagcagttga aacggcggtt
      541 tgaagccgag tttgacgaat tggaaaaaga aatcaaatgc aacggcaaac cgacattgtt
      601 gctttagcag aggacaaccg ggaggatgcc gccgtccgaa tcggacgtgc ggttttcgta
      661 ccggtacggg cgggcaggaa tgtccgcttt ttttgttcgg acgcgtttga atacccgttt
      721 gattccgtcc gtttgcaagg ggtatttccg ttcgggcgga aattatagtg gat
//
LOCUS       DAWKST010000270          754 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.270, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000270 DAWKST010000000
VERSION     DAWKST010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.270"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
BASE COUNT          190 a          188 c          200 g          176 t
ORIGIN      
        1 ccctgccgct ccctgtgcta cgcaacggtt gtgtgccttg ggtgggcgcg ggtggcggcc
       61 gggcggtttg ttgctatgat acgggagttc cgatatacaa gggtgggctt cagcccacca
      121 atcaacgacc gaaaaagaaa aaacgttggg ctgaagccca ctctacgcaa ttacgcaact
      181 gttatcgtgg ggatgacggg attttaggtt tttgtttttc tgtttttgcg ggaaggatgg
      241 gttttaggtt gcggtcattt atcggaaaag cagaaatcgc tccgccgtca ttcccgcgaa
      301 agcgggaatc tgaacacgtc cgtagggaaa cctatatccc gtcattccca cgaaagtggg
      361 aatctgaaca cgtccgtagg gaaacctata tcccgtcatt cccacgaaag tgggaatcta
      421 gaacctctga attttcagac aacctttgaa tattgccgcc gtcccgcgtt ctagattccc
      481 gcctgcgcgg gaatgacgat tcatcagttt cccgaaacgc ccgaaaaacc gaaaccgagc
      541 aaaccggatt cccgcctgcg cgggaatgac gatttataag tttcccgaaa cgcccaaaaa
      601 aaccgaaatc cgacaggcgg gatttttgct ttcgtgggga tgacgggatt ttaagttacg
      661 gtcatttatc ggaaaagtag aaatcgctcc gccgtcattc cagcgaaggc gggaatctga
      721 acacgtccgc acggaaacct atagtggatt aaca
//
LOCUS       DAWKST010000271          668 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.271, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000271 DAWKST010000000
VERSION     DAWKST010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.271"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(127..>668)
                     /locus_tag="WJL87_002049"
     CDS             complement(127..>668)
                     /locus_tag="WJL87_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ7800435.1"
                     /translation="SAVESRPAVPAAVQTPVKPAAQPPVQSAPQPAAPAAENKAVPAP
                     APQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGVDIAGNAGQPVLAAADGKVV
                     YAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDASRTQL
                     HFEVRQNGKPVNPNSYIAF"
BASE COUNT          151 a          191 c          186 g          140 t
ORIGIN      
        1 aaacacagtc aaacaaattt atatgcccca tcccttccga ataatttgaa aacacagccg
       61 ccaaaaacaa aaatgccgtc tgaaaacctt tcagacggca tttccaattt gatttcaggc
      121 agaaagtcag aacgcgatat agctgttcgg gttaaccggt ttgccgtttt gccgcacctc
      181 gaaatgaagc tgcgttctgg aagcgtcggt attgcccatc aaagcgacct gctgcccgcg
      241 tttgacctgc tggccttcgc cgaccagcaa tttttggttg tgcccgtatg cggtcaggaa
      301 ggaagaatta tgctggatga tgaccagatt gccgtatccc ctcaaaccgg aacctgcata
      361 aaccactttg ccgtcagccg ccgccaaaac gggctgtccc gcattaccgg caatatcgac
      421 acccttgttg ttgccgccga aatcggcaac cactttacct tgcgtcggac gctgccaaac
      481 aatgccgccg accgaacgcg tgccggaagg cgaagcggca ggagattgcg gggcgggcgc
      541 gggaaccgct ttattttccg cagcgggcgc ggcaggttgc ggcgcggact gcacaggcgg
      601 ttgcgcggcg ggtttcacag gggtttgcac ggcagccggt acggcgggcc tgctttctac
      661 ggctgagg
//
LOCUS       DAWKST010000272          652 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.272, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000272 DAWKST010000000
VERSION     DAWKST010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.272"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(51..536)
                     /gene="fxsA"
                     /locus_tag="WJL87_002050"
     CDS             complement(51..536)
                     /gene="fxsA"
                     /locus_tag="WJL87_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ7800436.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAATFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          193 a          211 c          119 g          129 t
ORIGIN      
        1 tcaaaccgga aaaatgccgt ctgaaccttc agacggcatt ccattcacat ttattcgtct
       61 tttttgtgtt cgatggcgtt tcgggaacga ttgccgccgt aaggctcttc aaccgtatat
      121 tccccctcga taatatcgtc atcgcgggaa aagccctctt ttctgcccga ttggttcatg
      181 ttgaaaaaat tttccgcacc tcctgcctgc aacactgcac ctcccttaaa cggcagcagc
      241 agcaataccg ccaacaccga ggatacgaat cccggactca tcagacacac cgccgccacc
      301 gtataacgga taggccacaa catctgataa acggataccc tcccgccgct tctcattgcc
      361 gcacccgcca ataaaagacc ggacagcccc gtatgcctga gcatcagcac accggcggca
      421 aaggttgccg ccatcagaaa cagcgtccaa ccgccgccca gccaatcggc aacccacaca
      481 atcgacataa tctccaaaaa cagcagcacc aaaaaaccga taccgaaaaa tctcattgac
      541 cgtcatcctt atatttaagt aaacagcaaa ccgcccgaac aagactccaa gcgacctgcc
      601 tgtaaatgat tacaaaacca tgtgcttcaa gccgaaacaa tgtgaaatca cg
//
LOCUS       DAWKST010000273          616 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.273, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000273 DAWKST010000000
VERSION     DAWKST010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.273"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..>616
                     /locus_tag="WJL87_002051"
     CDS             <1..>616
                     /locus_tag="WJL87_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS1655 family transposase"
                     /protein_id="HEZ7800437.1"
                     /translation="IYRYLRQDKSNGSTLWQHLRICSKPYRKRYGSTWTRGKVPNRVG
                     IENRPAIVDQKSRIGDWEADTIVGKGQKSALLTLVERVTRYTIICKLDSLKAEDTARA
                     AVRALKAHKDRVHTITMDNGKEFYQHTKITKALKAETYFCRPYHSWEKGLNENTNGLI
                     RQYFPKQTDFRNISDREIRRVQDELNHRPRKTLGYETPSVLFLNL"
BASE COUNT          198 a          168 c          136 g          114 t
ORIGIN      
        1 catttaccgc taccttcgcc aagacaaaag caacggcagc acgttgtggc aacatctcag
       61 aatatgcagc aaaccctacc gcaaacgcta cggcagcaca tggaccagag gcaaagtccc
      121 caaccgtgtc ggcatagaaa accgacccgc tatcgtcgac cagaaatccc gtatcggcga
      181 ttgggaagcc gacaccattg tcggcaaagg acagaaaagc gcattattga ccttggtcga
      241 acgcgttacc cgctacacca tcatctgcaa attggatagc ctcaaagccg aagacactgc
      301 ccgggcagct gttagggcat taaaggcaca taaagacagg gtgcacacca ttaccatgga
      361 taacggcaaa gagttctacc aacacaccaa aataaccaaa gcattgaaag cggagactta
      421 tttttgtcgc ccttaccatt cttgggagaa agggctgaat gagaacacca acggactcat
      481 ccggcaatac ttccccaaac aaaccgattt ccgtaacatc agtgatcggg agatacgcag
      541 ggttcaagat gagttgaacc accgaccaag aaaaacactt ggctacgaaa cgccaagtgt
      601 tttattcttg aatctg
//
LOCUS       DAWKST010000274          600 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.274, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000274 DAWKST010000000
VERSION     DAWKST010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.274"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..377
                     /locus_tag="WJL87_002052"
     CDS             <1..377
                     /locus_tag="WJL87_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7800438.1"
                     /translation="RLAARDIIRFPGFVNNVSEVIKEHHIFVLPSYYREGVPRSTQEA
                     MAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAEKMIYFIENREAVRLMGNASY
                     AIAKDKFDAEKVDLKLLDILKA"
BASE COUNT          147 a          148 c          146 g          159 t
ORIGIN      
        1 aacggcttgc cgcccgcgat attatccgtt tccccggttt tgtgaacaat gtttccgaag
       61 tgataaaaga acatcatata ttcgtattgc cgtcttatta tagggaaggc gttccccgaa
      121 gcactcagga ggcaatggcc gtcggcaggg cagtgattac gacggatgtc cccggatgca
      181 gggaaacggt cgccgacaag gtcaacggct tcctgatcga gccttggaat ccccgcatct
      241 tggccgaaaa aatgatttat tttatcgaaa acagggaagc cgtccgcctg atggggaatg
      301 caagttatgc gattgccaaa gataaattcg atgccgaaaa agtcgatttg aaattgctcg
      361 atattttgaa ggcgtaaaca aggctgcccg cttttgagtt tcggtcattt ctgataaacc
      421 cccgtcattc ccgccacacc cccgtcattc ccgcgaaagc gggaatccag gttcgttgag
      481 tttcggtcat ttccgataaa ttcttgcaac tttgagtttc tagattccca ctttcgtggg
      541 aatgacggcg gagggttgct gttttcccga caaattcccg ccatctaaaa tctcgttatc
//
LOCUS       DAWKST010000275          545 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.275, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000275 DAWKST010000000
VERSION     DAWKST010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.275"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(464..>545)
                     /locus_tag="WJL87_002053"
     CDS             complement(464..>545)
                     /locus_tag="WJL87_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ7800439.1"
                     /translation="IGRDKLFIHPRLMELLRGEGNSFTSF"
BASE COUNT          150 a           99 c          121 g          175 t
ORIGIN      
        1 taggtttcca tgcggacgcg ttcggattca cgactgcgcg gaaatgacgg gattttggtg
       61 tattccctaa aaaaataaaa aaacatttgc aactttgtta aaaataaagg ctgtgtttta
      121 acgatgtgtt gatatttaat tttagaaagg tagctattta atagttacct tttcttattt
      181 aaaaatagct ttctcaaatt ccatgaacgc ctcaatacga tatgcagatg ctctatcgaa
      241 attaagtttc aacattttgt ttattaaaca ttttatttta gccatttttc aatatacccc
      301 caaatatacc cccaatttgc acaagtcaaa attttaggca ggggttttgt tgctcccatg
      361 atacgcgggc ggatgccggc ttttcgcggg ggaaatacaa ggggtctcgg ttcgggtgtc
      421 aaaatccctg tttcgtgtta gtcatgtggg ggggaagagg gggttagaat gaagtaaagc
      481 tgttgccctc tccccgcaat agttccatta ggcgcggatg aatgaatagt ttgtccctgc
      541 cgatg
//
LOCUS       DAWKST010000276          540 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.276, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000276 DAWKST010000000
VERSION     DAWKST010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.276"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..248
                     /locus_tag="WJL87_002054"
     CDS             <1..248
                     /locus_tag="WJL87_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7800440.1"
                     /translation="DGIMRKACRNRPLTEAQTKRNRYLSKTRYVVEQSFGTLHRKFRY
                     ARAAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAAA"
     gene            431..>540
                     /locus_tag="WJL87_002055"
     CDS             431..>540
                     /locus_tag="WJL87_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800441.1"
                     /translation="MHLRHILPPTAEALMRSRYSAYVLHLIDYSGLTKTST"
BASE COUNT          138 a          137 c          149 g          116 t
ORIGIN      
        1 tggacggcat tatgcgcaaa gcctgccgca accgtccgct gacggaggca caaaccaaac
       61 gcaaccgata tttgtcgaag acccgttatg tggtcgaaca aagcttcggt acgctgcacc
      121 gtaagttccg ctatgctcgg gcagcctatt tcggactgat taaagtgagt gcgcaaagtc
      181 atctgaaagc gatgtgtttg aacctgttga aagccgccaa caggctaagt gcgcccgctg
      241 ccgcctaaaa agcgaccgga tgcctgatta tcgggtatcc agggaagatt aagggggtat
      301 ttgggtaaaa tcaggaggta tttggggcga aaacagccga aaacagccga aaacctgtgt
      361 ttgggtttcg gctgtcggga gggaaaggaa tgttgcaaag gtctcaagct atgtcggctg
      421 ctgccgccca ttgcaccttc gccatatcct accgcccacc gctgaagccc tcatgcgttc
      481 gcggtacagc gcgtatgtgc tgcaccttat cgactatagt ggattaacaa aaaccagtac
//
LOCUS       DAWKST010000277          534 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.277, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000277 DAWKST010000000
VERSION     DAWKST010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.277"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
BASE COUNT          142 a           87 c          163 g          142 t
ORIGIN      
        1 ccgtcggtgc ttttttgttt ggaagggaaa tggctggaaa aagacctgcg ctatatcggg
       61 gaatgaatcg acgcaaagaa aatgccgtct gaacatgcgt tcgggcggcg ttttgttggg
      121 ggggtatcgg agcggaacgt ctgaaaaagg gtttcaggcg gtctttgggc gtgtggtaac
      181 agtcgaaaac gtgataaggc tacctgaaaa gtttgggaga ttttcaggta gtctttggta
      241 ttgggcgcaa cagacgcagg tacagattag gcggtgtgcc gtaatcgtac gaatgccgat
      301 tcaacctaag cagacatcag tatttaggaa gtggatgttt gatggagcaa aggttgtacg
      361 aagggtggaa ggcaacctgt gggtgtttgg tatggtcgcg cttgaaaaaa cgtgttttaa
      421 gggacaaatg ccgtctgaaa atcggtttca gacggcattt tctgtttatt taaagcaaac
      481 aggaaaaggc agcaatattc tgcagtcttc ctattcacac aagcgtttta tagt
//
LOCUS       DAWKST010000278          529 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.278, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000278 DAWKST010000000
VERSION     DAWKST010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.278"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..476)
                     /locus_tag="WJL87_002056"
     CDS             complement(<1..476)
                     /locus_tag="WJL87_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ7800442.1"
                     /translation="MIASRLSGNISSAIQEHKDGKVNINRFQNILADLYSLGGLGSTL
                     IEKNGNMQSWGIPLAIAGDIIAATAIATGDTGTISTEEFYNFDNWKGFGYELFEDWSR
                     WVYDWLPDGWNLWKELDRNRSGQYHIYDPLALDLDGDGIETVAAKGFSGSLFDHNG"
BASE COUNT          137 a          128 c          104 g          160 t
ORIGIN      
        1 ttgccgttat ggtcgaagag gctgcctgaa aagcctttgg cggcgactgt ttctatgccg
       61 tcgccgtcta ggtctagggc gagggggtcg tagatgtggt attggcctga acggtttctg
      121 tccaattctt tccacagatt ccagccgtcg ggcagccagt cgtataccca acgagaccag
      181 tcttcaaata gctcataccc aaaacctttc cagttgtcaa aattataaaa ttcctctgta
      241 gatatcgtac cagtatctcc tgtggcaata gccgttgctg caattatatc tccagcaatt
      301 gctaatggaa tcccccaact ctgcatattt ccattcttct ctattaatgt acttcctaac
      361 cctcccaatg aatataaatc cgctaaaata ttttgaaaac ggttgatatt aactttacca
      421 tctttatgct cctgaatagc tgaagatata tttccagaga gacggcttgc aatcacagag
      481 gcatttgttc cgtttacaat atgcgatgac gtactacctg gtgcaatat
//
LOCUS       DAWKST010000279          515 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M29132_Neisseria
            meningitidis SAMN10276031-rid8982803.denovo.279, whole genome
            shotgun sequence.
ACCESSION   DAWKST010000279 DAWKST010000000
VERSION     DAWKST010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10276031
            Sequence Read Archive: SRR8200202
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676202.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/19/2022 00:30:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,058
            CDSs (total)                      :: 2,008
            Genes (coding)                    :: 1,942
            CDSs (with protein)               :: 1,942
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 39 of 66
            Pseudo Genes (internal stop)      :: 10 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10276031-rid8982803.denovo.279"
                     /isolate="M29132_Neisseria meningitidis"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2014"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(1..146)
                     /locus_tag="WJL87_002057"
                     /pseudo
     CDS             complement(1..146)
                     /locus_tag="WJL87_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
     gene            complement(169..354)
                     /locus_tag="WJL87_002058"
     CDS             complement(169..354)
                     /locus_tag="WJL87_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7800443.1"
                     /translation="MGVCLDACCSAAGIARQIGKGGEIHGTLAFSLLFLSLGHIGMVA
                     YHHIKGESVLPRMLGKS"
BASE COUNT          122 a          137 c          132 g          124 t
ORIGIN      
        1 tccactatat tttgatgcag acgtgtaccg atgtagcccg gctgggtttt caggaaatcg
       61 cgtgatttct gccaagcagc caaagtttcg gcttctttgc cctgcggcac ttcaaaggtg
      121 ttgatgagta cagcactgtt ctgcatgatt taaatttaat ccactatatt aagattttcc
      181 gagcattcga ggcagcacgg attcgccttt gatgtggtgg taggcaacca ttccaatgtg
      241 tcccaacgac aaaaacagca atgaaaatgc cagcgtgccg tgaatttctc cgcccttgcc
      301 gatttgacgg gcaatgcctg cggcggaaca gcaagcatca aggcatacgc ccaatgctca
      361 atttggtgac ggcggtgttg gcggggcggt ttttacgttg gcggattccc cacccttgta
      421 tatcggaact cctgtatcat agcaacaaac cgcccgaccg ccacccgcgc ccaccaaaag
      481 cagacaaccg ttgcgtagct cagggagcgg caggg
//
